LOCUS       DASWHZ010000001         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_739107, whole genome shotgun sequence.
ACCESSION   DASWHZ010000001 DASWHZ010000000
VERSION     DASWHZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739107"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VGA49_00005"
     CDS             complement(<1..800)
                     /locus_tag="VGA49_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664194.1"
                     /translation="MAEEEISEGGLKFGYWYASHRQSLKKWLIIFLIGLNLIFYGYSL
                     YKVIDLYVLQADSYQRQLQTLPLDLVNYRSFRAKDQPQSLQIIGISVLSTGQRTYDLL
                     ARVRNPNPKWQAEFDYQFFAQGLETKTYQGFLLPGDEKFLVDFALASDRGIVQPRLEF
                     RNLKWKKILQFEQVKADQINFLTENIKFIPARATELGEAVAVSQTTFDVTNLSPYNYW
                     QAGFYIVLFSGNRAVGINFISLDQFLSGDKRSVTVNWFETLPAITKIEV"
     gene            complement(1002..>1655)
                     /locus_tag="VGA49_00010"
     CDS             complement(1002..>1655)
                     /locus_tag="VGA49_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664195.1"
                     /translation="VPLTEDVMDGKQDGELRPKFTRATVSLYGGLGFNLGPDWFRFDV
                     DFLAGGFSPSQADELEDDFHRQFTRKPISPLDPWFTRHQKGFQGFETMTRASLDIRAL
                     GTRFHFWGYGLYNAEQHNRHFEARGTIAVWNDPDSLIQTVEVGGGFIYDRLRTPNMIR
                     LKSAGISSVFTVTDWCQVSCDLFYGQEDILAGDDNHNNRLKSFHGLTVVVGVSLIYW"
BASE COUNT          446 a          433 c          376 g          400 t
ORIGIN      
        1 acttcaattt tagtaatggc tggcagagtt tcaaaccagt taacggttac tgaccgcttg
       61 tctccggata aaaactgatc caggctgata aaattaatgc cgaccgcccg gttgccgcta
      121 aacaacacaa tgtagaaacc agcctgccag taattatagg ggcttaaatt ggttacatca
      181 aaagtcgtct ggctgacggc gaccgcctcg cccagttcag tcgccctggc cggaatgaat
      241 ttaatgtttt ccgtcaaaaa atttatctga tcggctttga cttgttcaaa ttgtaaaatc
      301 tttttccatt ttaaatttct aaactccagc cggggctgaa caatcccccg gtcgctggcc
      361 agggcgaaat caaccagaaa cttctcgtcg cccggcagta aaaatccctg atacgttttg
      421 gtttccagtc cctgggcgaa aaactgataa tcaaactcag cctgccattt gggattgggg
      481 ttgcggacgc gagccagtaa atcataggtt cgctggccgg ttgataaaac gctgatgccg
      541 atgatttgta aactttgcgg ctggtctttg gcccgaaaag accggtaatt aaccagatcc
      601 agcggcaaag tttgcaactg acgctgataa ctgtcagcct gtaaaacata gagatcaatc
      661 actttataca aactatagcc gtaaaaaata agattcaggc caattaaaaa aataatcagc
      721 catttcttca ggctttgacg atggcttgcg taccagtagc cgaattttaa gccgccttct
      781 gaaatttctt cttctgccat attaaattat tataccaaaa attgtccaaa tcaccaaccg
      841 caccgtcccg ccttggcggg acggtatccg gttgttgatc ccgccgttgg cgggatttat
      901 tttttgcccc cattcatcct tgcggcaagc cgcaaggtat tctgggcaaa aaataaaaaa
      961 agaggaacct gacagttcct cttgggtgaa gatctcctgc ctcaccagta aatcagactg
     1021 acgccaacga cgaccgtcag tccgtgaaaa gacttcagcc ggttgttgtg gttatcatca
     1081 ccggccaaaa tgtcttcctg accgtaaaac aggtcacagc tgacctgaca ccagtcggtc
     1141 acggtaaaaa cagacgagat gccggccgac ttgagccgaa tcatattcgg cgtccgcagg
     1201 cggtcataga tgaaaccacc gccgacttca accgtttgaa tgaggctgtc cggatcgttc
     1261 cagacggcaa tcgttcccct ggcctcaaaa tgtcggttat gctgttcagc attgtagaga
     1321 ccatagcccc aaaaatgaaa ccgggtgccc aaagctctga tatcaagcga ggcccgggtc
     1381 attgtttcaa acccctggaa ccccttttga tgcctggtga accagggatc aagcgggctg
     1441 atcggctttc tagtaaactg gcgatgaaaa tcatcctcca gttcgtcagc ctggcttggc
     1501 gaaaatccac cggccaaaaa atcaacgtca aacctgaacc agtccggccc gagattgaaa
     1561 ccaaggccgc cgtacaggct gacggtcgcc cgggtgaatt tgggccgcag ctcgccgtcc
     1621 tgtttgccgt ccatcacatc ttcagtcaaa ggaac
//
LOCUS       DASWHZ010000002         9686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3331819, whole genome shotgun sequence.
ACCESSION   DASWHZ010000002 DASWHZ010000000
VERSION     DASWHZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331819"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VGA49_00015"
     CDS             <1..750
                     /locus_tag="VGA49_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX9664196.1"
                     /translation="LSLIKEMPAGRKKIMTKTVKSLNRPEAYQFIRNEIKKGRQAFVI
                     CPLIDPSDNLGVKSVTEESARLKTKVFPDLKINLLHGQLKTDDKEKIMKDFLDKKFDL
                     LVATSVIEVGIDIPNATIMVIEGAERFGLAQLHQFRGRVGRSDHQSYCLLFPTDDSLA
                     ALERLRALENCQNGFELAEKDLELRGPGEIYGTKQSGLPELKIATLTDYQAMKLAMEE
                     AKTLLSDDPELKKHPLLKGKVDEFTKSVHLE"
     gene            complement(880..2229)
                     /gene="radA"
                     /locus_tag="VGA49_00020"
     CDS             complement(880..2229)
                     /gene="radA"
                     /locus_tag="VGA49_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9664197.1"
                     /translation="MNILYICEKCDAQFSKWSGRCLECGAWGSLTQANYSESKKSVSA
                     GEIVVKDLISFGEGNEANQKRIKTNISELDDVLGGGIVPGSLILLGGEPGIGKSTIVL
                     QILQNLSPITNPLYPGLYVSGEESAGQIKMRIDRLGLKSDNLKFLDETNIETIISTLE
                     KHKPALAIIDSIQTAYSAEVASPMGSVNQVRVCATKLLEAAKKSNVPVIVIGHVTKDG
                     QVAGPKTLEHLVDVVLYLEGDRYQSLRLLRTVKNRFGSTNQVGVFEMTNQGLAPVANP
                     SKIFLANKQDNISGSVIACVLEGSRPFLIEVQALLTKASFGYPQRKTNGFDLNRLQLL
                     TAVLIQRANLPLDKYDIYLNIAGGFKVTEPSADLAVCLAIASSLKNKPVASDLVCLGE
                     VGLGGEIRSVNQVEKRVEEISKLGFKQIVLPKQKLGDNKIKFSFVKNVGESLEMLEL"
     gene            complement(2640..3797)
                     /locus_tag="VGA49_00025"
     CDS             complement(2640..3797)
                     /locus_tag="VGA49_00025"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664198.1"
                     /translation="MFRNKKIILKILSVFFLVFVFGILLSGLVLAQETASIKLNVPFG
                     GLGTASNYAEYIAALYKFGVAAAGVVAVVMIMIGGIIWIVAAGNASKIGQAQNLITNA
                     IIGLIMILGSYVLLNTINPDLVKLRVPELETIIGKRTASTVNKCCVSESTGKEPFYNL
                     RADNCLPDEMLLTRTLPCIDFAKGPSDCAYPDLEKPGEEKCEKLSYNECQAKNGTFFD
                     CTNKYYVALCQVGYEVPEKGCWCGLKAGKAAPNKVISGYCCGSQEGEFWQTVPCSQAL
                     TKQPEGEVCGENPDCMDGLICNTGDKRCAKLGTENAFCGTDPDCGGGLVCHRNNRVCV
                     NRCAAKGESCSRGSSAQSYDICCEANSVDPNVYIECAGGSGTVWGICVLRR"
     gene            complement(3811..5064)
                     /locus_tag="VGA49_00030"
     CDS             complement(3811..5064)
                     /locus_tag="VGA49_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX9664199.1"
                     /translation="MNWRRILIIAGIVVIIGIVGFLVYYFVFRPPVAPIAPPPPTAFP
                     PINVPVGLPPAIEREPVAVSPEEAVQVVPPELLLPPEVPPPSIADFATGGLTRVSQVN
                     NIRTAGVTLASDGANLNFYNRDTGQFYQVTPDGEMKLLTDRIFRQVENIVWSPNKNET
                     ILEFPDGSNVLFNMATDQVITLPRHWEEFSFSGNGAKIAAKSLAADPEDRWLIVSNPD
                     GSSARLIEPLGDNADRVDVDWSPNNQVIATFTESIDFDRQELYFVGQNDENFKLTVLN
                     GRGFEGQWAPQGDRLLYSVYNSETDFNPQLWIVDARGENIGNNRKPLNIRTWADKCAF
                     SSDNINLICGVPTQLPTGAGIIPSVANDISDVIVRINTQTGSVALAALTDKDYTVKDP
                     VLSADESKLYFLNQFDQLIYSVNLK"
     gene            complement(5081..5605)
                     /locus_tag="VGA49_00035"
     CDS             complement(5081..5605)
                     /locus_tag="VGA49_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664200.1"
                     /translation="MAATATLPAAPAGGATAQQQATTRQFDRAKRNAKKKDQAEKPAE
                     AGGEISLGEKTNLAGQLVTARLLNSAWLAIPETFGLSLLYVNFHFVMAHIVRSSLFCM
                     LGQEWQFGGKMKYTPSSALMWVEIIILIAINIMMIAMLVVSLAMFILMILPAVAPGVA
                     LGILGSELLNLIGF"
     gene            complement(5612..8092)
                     /locus_tag="VGA49_00040"
     CDS             complement(5612..8092)
                     /locus_tag="VGA49_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX9664201.1"
                     /translation="MRTNITDKFTTHFKKTLVRASNFASANKLAEVQPEHVLYGLLTQ
                     RGSIGCEILTQIKLTSDKVRAKIVSRPVVSQAAGRTPETVISQETKQLIYKAVLTANL
                     YQHKYVGTEHLLASLLEIKNPVIRKIFSGYKIDFKTLESKILSLLKSTSKFPDLTETF
                     SALKEHQLAERGYETSRGSVLDAFTTDLTDKKIQKNIDPVIARELEIERLIQILSRRT
                     KNNPLLLGDPGVGKTAIVEGLAKRIINQEVPDILLNKKILSLDLGLIIAGTMYRGEFE
                     SRLKQLLEEIKHDPNVILFIDEIHNIIGTGSTSGTLDMANLLKPALARGEIRCIGATT
                     YEEYRKYIQKDIALERRFQPIKVAEPTVQETVSILDGLKQNYEQFHRVLIDQAALNSA
                     VNLSDRYIQDRFLPDKAIDLIDEACSRVKVKNQNSQLQRQINTAEKHLEEVKYRKQKA
                     VFNEDFKKAIHYKKQEEILVSELVALQKIQEKEADKFIGQVTAADIQGVISKMTGIPL
                     ENLKQREKQKILGLEDSLNQKIIGQREAVRLISQVIRRSRTGLCSPQRPIGSFMFLGP
                     SGVGKTHTAKTLAGALFENKNSLIRIDMSEFGEKFNISKLIGSPAGYVGYQESGTLTE
                     AVKRQPYSVVLFDEIEKAHRDVFNLLLQILDDGYITDAGGKRVDFKNTIIIMTSNLGS
                     ERFKNSAGLGFNGSSSSNHSLKDIKTDLQKELTGYFRPEFLNRIDQVVIFELLTSKDL
                     EAVAELQIKELISRLKQQKIEIQVSPAVFRFIGQKKTEEDLGARAIIKNIRELIENPL
                     SEFLLREEINEKEPIKISLKNKNIIFKN"
     gene            complement(8302..9183)
                     /locus_tag="VGA49_00045"
     CDS             complement(8302..9183)
                     /locus_tag="VGA49_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9664202.1"
                     /translation="MHEQILSNKRKTTFLILLFTIFVIALGWIFGELTGSGQLGIIIA
                     VIVSVVMTLFSYFSGDKVALLAAGAKEIKKDDNPYVYRLVENLSITAGLPVPKVHIIN
                     DPAPNAFATGRDPKHASVALTTGLIERLENEELEGVIAHELAHIQNYDIRLMMIVIVL
                     LGVVVLLSDWLIRISFYSGRDDRKGGAGAIIIIVGIILAIMSPIFAKLIQLAVSRKRE
                     YLADASGSLLTRYPEGLASALEKISVYHQPLRRANNATAHLYINNPFGTTKRFLSGLF
                     STHPSITERIKALRGMT"
     gene            complement(9369..>9686)
                     /locus_tag="VGA49_00050"
     CDS             complement(9369..>9686)
                     /locus_tag="VGA49_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9664203.1"
                     /translation="QAQGMAEKSKAENMLSDTLKSLFAVAENYPDLKATQNFIQLQDE
                     LVDTENKIQAARRFYNNNVRLFNTKLQVFPTNMIGRMLGFMAKEYFEVEDEKEREAVR
                     VKF"
BASE COUNT         2695 a         2312 c         1952 g         2727 t
ORIGIN      
        1 ttatctttaa tcaaagaaat gccggctggc cggaaaaaaa taatgaccaa aacagttaaa
       61 tcgctcaacc ggcctgaagc ttatcaattt atccgaaatg aaatcaaaaa aggccggcag
      121 gcctttgtca tctgtccgct gattgatccg tctgataacc tgggagtgaa atcagttact
      181 gaagaatccg cccggctgaa aacgaaagtt tttcctgatt taaaaatcaa tctgcttcac
      241 ggccagctca aaacggacga caaagaaaaa atcatgaaag attttctgga taaaaaattt
      301 gatttgctgg tggccacttc agtcattgaa gtcggaattg atattcccaa cgccacgatt
      361 atggtgattg aaggggcaga aagatttggc ctggcccagc ttcaccagtt ccgcggccgg
      421 gtcggccgga gcgatcacca gtcatattgt ttactatttc caactgacga cagcctggcc
      481 gctctggaac gtctgcgcgc tttggaaaac tgccaaaacg gctttgaact ggctgaaaaa
      541 gacctggaac ttcgcggccc gggcgaaatt tacggcacca aacagtccgg tctgcctgaa
      601 ctgaaaatcg ccactttaac agattaccag gcgatgaaac tggcgatgga ggaagctaaa
      661 actcttcttt cagacgaccc tgaattaaaa aaacatcctt tgctgaaagg aaaggtggat
      721 gaatttacaa agtcagtgca tttagaataa agtttatagt tattagtttt agcggccggt
      781 atagtctttg gttattagtt tttagatatg ttcataaacc aaagactaaa cctttaaacc
      841 atactggccg ccatgactaa aaaccaaaaa ctaaaagact tataattcca acatctccaa
      901 tgattccccc acattcttca caaaactaaa cttaatttta ttatccccta acttttgctt
      961 cggcaggaca atctgcttaa accccagctt agaaatctcc tccactcgtt tctcaacctg
     1021 attaaccgac ctgatctcgc ctcccaaacc aacttcgccc aaacaaacca gatctgaagc
     1081 cactggcttg ttttttaatg atgaagcaat cgccagacaa accgccaaat cggccgacgg
     1141 ttcagttacc ttaaagccgc cggcgatgtt gagataaata tcatatttat ccaaaggcag
     1201 gttggcccgc tggatcaaaa cggccgtcaa caactgcaaa cggttcaaat caaaaccatt
     1261 ggtctttctt tggggatagc caaaactagc tttagtcagc aaggcctgga cttcaattaa
     1321 aaaaggccgg gaaccttcca ggacgcaggc aatgactgat ccggaaatat tatcctgctt
     1381 gttggccaaa aatattttgg aaggattggc caccggcgcc aaaccctgat tggtcatttc
     1441 aaacactccg acctggttgg tggagccaaa acggttttta actgtccgca atagtcgtaa
     1501 cgactgatac cgatcgcctt ctaaatagag aacaacatct accaaatgct ccaaagtttt
     1561 gggtccggcc acctggccgt ctttagtgac atgcccaatg acaatcaccg gcacattgga
     1621 ttttttggcg gcttccaaaa gtttagtggc gcagactctg acctgattca cgctgcccat
     1681 gggcgaagcc acttccgctg aataagctgt ttgaatggaa tcaataatgg ctaaggctgg
     1741 tttatgcttt tccagcgttg aaataatcgt ttcaatattg gtttcgtcca aaaattttaa
     1801 attgtcagat ttcaggccca gccggtcaat ccgcatttta atctgcccgg ccgattcttc
     1861 cccagagaca tataaaccag ggtatagggg attggttata ggggatagat tttgtagaat
     1921 ctgtaaaacg attgtggact tgccgattcc cggctcgccg cccaataaaa tcaaacttcc
     1981 cggcacgatc ccgcctccta aaacatcatc caattcgctt atattcgttt taattcgctt
     2041 ttgattcgct tcatttcctt ccccaaaact tattaagtct ttcaccacaa tctcgccggc
     2101 tgaaactgat tttttagatt cgctataatt agcttgcgtt aaactacccc aggcgccgca
     2161 ttccagacaa cggccggacc atttggaaaa ctgggcgtcg catttttcgc agatgtagag
     2221 gatgttcata ttttattgac gacagagttt tgcttctatc cgctttcatt aacgatttaa
     2281 tattagctga ttatcagctt aaaataaaga acagcccctt gtctagggcc tgcccgttat
     2341 ttttattata aaacgttaat caattatttg aaaagttaac tctaaagatg acgatccgga
     2401 aataatatat tttgttttat tagaaaagga acaatttgtt tttcaagtat tctgaagatg
     2461 tacatagtct taattttagc agggttatta ttaaatttat tttaagttta gcagggttat
     2521 taagacttgt caaaactggg catcgcattt ttggcagagg tagaggatgt tcatagtatg
     2581 gccactttaa caaaaaacaa cccgtgggtc gatgaggctg attttttaat aaatgttatc
     2641 tacctccgta atacacaaat gccccaaact gtaccagaac cacctgcaca ttcaatatat
     2701 acattaggat ccactgagtt tgcttcgcaa cagatatcat atgattgagc ggatgaaccc
     2761 cgtgaacaac tctctccctt ggctgcacaa cgatttacac acactcggtt attacgatgg
     2821 caaaccaacc cccctccaca gtcagggtca gttccgcaaa aagcattttc tgtcccaagc
     2881 tttgcacatc ttttatcgcc agtattgcaa attaatccat ccatacagtc gggattttcg
     2941 ccacaaacct cgccttcagg ttgtttggta agagcttgac tgcatggaac cgtttgccaa
     3001 aactctcctt cctgtgaacc acaacaatac ccgcttataa ctttatttgg agctgcttta
     3061 ccagctttta atccgcacca acaccctttt tcaggcacct catacccgac ctgacaaaga
     3121 gccacataat atttatttgt gcagtcaaaa aatgttccat ttttagcttg acattcatta
     3181 tagcttaatt tttcacattt ttcttcacct ggtttttcaa gatcaggata agcgcaatca
     3241 gatggacctt tggcgaagtc tatacaagga agcgttcttg ttaaaagcat ttcatctgga
     3301 agacaattat ctgctcttaa attataaaat ggttccttgc cggtactttc actgacacaa
     3361 catttgttga ctgttgatgc tgttcgtttt ccaataatcg tctccagctc cggcactctc
     3421 aatttcacca aatccggatt gattgtgttt aataaaacat aagaccccaa aatcataatt
     3481 aagccaatga tggcgttggt aatcaaattc tgggcctggc caattttaga agcgttgccg
     3541 gcggccacga tccagataat tccgccaatc ataatcatca ccaccgccac gacgccggcg
     3601 gccgccaccc caaacttata aagcgccgcg atatactccg cgtaattgga agccgtgccc
     3661 agccccccaa aaggcacgtt tagtttaatg gaggccgtct cctgggccaa aaccaaaccg
     3721 ctcaataaaa ttccaaaaac aaaaaccaga aaaaaaactg ataaaatttt taaaattatt
     3781 tttttatttc taaacataaa aggcgtcagc ttattttaaa ttaacgctgt aaattaactg
     3841 atcaaactga ttaagaaaat acagcttcga ttcatccgct gataagaccg gatctttgac
     3901 cgtgtaatct ttatcggtca gcgcggccag ggcgactgat ccggtttggg tgttaattct
     3961 gacaatgaca tccgatatat cattggccac gctgggaatg ataccggcgc cagtcggcag
     4021 ttgagtcggc accccgcaga ttaaatttat attatcactg ctaaaggcgc acttatcagc
     4081 ccaggtccgg atatttaaag gtttgcggtt attgccaatg ttttctcccc tggcgtcaac
     4141 aatccacagc tggggattaa aatccgtttc agaattgtaa acactgtaca gcaggcggtc
     4201 tccctggggc gcccactgcc cctcaaagcc ccggccgttt aaaacagtca gtttaaaatt
     4261 ttcgtcgttc tgccccacaa aataaagttc ctgccggtca aaatcaatgc tttcggtgaa
     4321 agtggcgatc acctgattgt tgggcgacca gtcaacatca actctgtcgg cgttatcacc
     4381 caacggctca atcaaccggg cgctggagcc gtccggattg gaaacaatca gccagcggtc
     4441 ttccggatca gccgccaggc ttttggcggc tattttggcg ccgttgcccg aaaaagaaaa
     4501 ttcttcccag tgtcgcggca gggtaatcac ctgatcagtg gccatgttaa ataagacatt
     4561 ggacccgtcg ggaaattcca gaatggtttc atttttatta ggcgaccaga caatattttc
     4621 cacctgacgg aatatccggt cagtcagcag tttcatttcg ccgtcaggcg ttacctgata
     4681 aaactggccg gtatcgcgat tataaaaatt caaattagcg ccgtcactag ccagagtaac
     4741 gccggctgtc cggatgttat taacctgact gactctggtc agcccgccgg tggcaaagtc
     4801 agcgattgag ggcggtggga cttccggcgg caataataat tccggcggca cgacctggac
     4861 ggcctcttcc ggactgacag ccacgggctc ccgttcaatg gccggcggca ggccgaccgg
     4921 gacgttaata ggtggaaagg cggttggcgg cggcggcgcg attggcgcca ccggcggccg
     4981 gaaaacaaag taataaacca aaaagccgac aataccaatt atcacgacta tgccggctat
     5041 aattaaaatt cttctccaat tcataattta tttttatttt ctaaaatcca atcaaattta
     5101 acaattcact tcccaatatc cccaaagcca cgccgggcgc gactgccggc aaaatcatta
     5161 atatgaacat cgccagtgaa acgaccagca tggctatcat catgatattg atcgcgatca
     5221 aaataatgat ctctacccac attaaagcgc tgctgggggt gtatttcatc ttaccaccaa
     5281 actgccattc ctggccaagc atgcaaaaca aactgctcct gacaatatga gccatcacaa
     5341 aatgaaaatt aacataaagc aggctcaagc caaaagtttc cgggatagcc agccaggctg
     5401 aattaagcaa acgggcggtc accagctggc cggccaaatt agttttctcg cctaaactta
     5461 tttctccacc cgcttcagct ggtttttcag cctgatcttt ctttttggcg tttcttttcg
     5521 cgcgatcaaa ttgccgggtg gtcgcctgct gttgagcggt cgctccgccg gccggcgccg
     5581 ctggtaatgt ggctgtcgcg gccatagtct gttaattttt aaaaattatg tttttgtttt
     5641 tcaaactaat tttaatcggc tctttttcat tgatctcttc ccgaagcaaa aattcagaca
     5701 gcgggttttc aatcagctcc cggatatttt ttataatcgc ccgggcgcct aaatcttctt
     5761 ctgtcttttt ctggccaata aacctaaaaa ccgccgggct gacctgaatt tcaatttttt
     5821 gttgttttaa acggctgatc agttccttga tctgtaattc agccaccgct tcaagatctt
     5881 ttgaagttaa aagttcaaaa ataaccacct ggtcaatccg gtttaaaaac tccggccgaa
     5941 aatatccggt cagctctttt tgcaaatcag tcttaatatc tttcagactg tggttagaag
     6001 acgatgaacc attaaacccc aggccggctg aatttttaaa ccgctccgac cccagattgg
     6061 aagtcataat aataatcgtg tttttaaaat ctacccgctt gccgccggcg tcggttatat
     6121 aaccatcatc caaaatttga agcaatagat taaagacatc ccggtgggct ttttcaattt
     6181 catcaaacaa aacaactgaa tacggctggc gcttaaccgc ttctgtcaaa gtcccgcttt
     6241 cctgatagcc aacatagccg gctggcgaac cgatcagttt ggaaatgtta aacttctccc
     6301 caaactcgga catgtcaatc cggatcaatg aatttttatt ttcaaataga gcgccggcca
     6361 gggttttggc ggtatgagtc ttgccgactc cggacggccc cagaaacata aatgagccaa
     6421 tcggccgctg gggcgagcat aatccggtcc ggcttcggcg gataacctgg ctgattagtc
     6481 tgacggcctc cctctgtccg ataatttttt gattcaaact gtcttccagt cccaaaattt
     6541 tttgcttctc ccgctgtttt aaattttcca gcggaatacc ggtcatttta gaaataaccc
     6601 cctgaatatc agcagccgtc acctggccga taaatttatc ggcttctttt tcctgaattt
     6661 tttgcaaagc gaccagttca gaaactaaaa tttcctcctg ctttttataa tgaatagctt
     6721 ttttaaaatc ttcattaaaa accgcttttt gcttgcgata tttaacctct tccagatgtt
     6781 tttcagctgt attgatctga cgctgaagct ggctgttttg gtttttaact ttaacccgcg
     6841 aacaagcttc atcaatcaaa tctatcgctt tgtccggcaa aaacctgtcc tgaatgtaac
     6901 gatcagacag attgaccgcg gagttaagag cggcctgatc aattaaaacc cggtgaaact
     6961 gctcatagtt ttgtttgagg ccgtccaaaa ttgaaaccgt ttcctgaaca gtcggctcgg
     7021 ccactttaat cggctgaaac cgccgttcca gagcgatatc tttttggata tatttccggt
     7081 attcttcata agtcgtcgcg ccaatacagc gaatctctcc ccgggccaga gccggcttga
     7141 gcagattggc catgtccagc gtgccgctgg ttgagccggt cccgataatg ttatgaattt
     7201 catcaataaa caaaataaca tttggatcat gtttgatttc ttccaaaagc tgtttcagcc
     7261 ggctttcaaa ttctccccgg tacatcgtcc cggcaatgat cagccccaaa tccagcgaca
     7321 atattttttt attcagtaaa atgtcaggca cttcctgatt aatgatcctt ttggccagcc
     7381 cctccacaat ggccgtttta ccgactccgg gatcacccag cagtaaagga ttatttttgg
     7441 tccggcggga cagaatctga atcagtcttt ctatttccag ctcccgggca ataaccgggt
     7501 caatattttt ttgaattttc ttgtcagtca aatcagtcgt aaaagcatca agaacgctgc
     7561 cccgggaagt ttcgtagccc ctttcagcca gctggtgctc tttcagggcg ctgaaagttt
     7621 ccgtcagatc gggaaatttt gaagtgctct tcaataaact taaaattttt gattccaaag
     7681 ttttaaaatc aattttataa cctgaaaaaa ttttcctgat aaccggattt tttatttcca
     7741 gcaggctggc cagcagatgc tcggtgccga catatttatg ctggtataaa ttggccgtca
     7801 gaacagcttt ataaattaac tgcttagttt cctggctgat aactgtttcc ggcgtccggc
     7861 cagcggcctg actgacaacc ggccttgaaa ctatttttgc cctgaccttg tcgctggtca
     7921 gtttaatttg ggttaaaatt tcacagccaa tactccctct ctgcgtcagc aaaccataca
     7981 aaacatgttc cggctgaact tcagccagct tgttggcgct ggcaaaatta gacgcccgga
     8041 ctagcgtctt tttaaagtga gtcgtgaatt tgtcggtaat attcgttcgc ataataatcg
     8101 attctaatat actttaaaaa aatccaaaat tcaaaattct aagttccaaa taaattataa
     8161 attcaaattt tttaaattca aaaccttgaa taagggcgtt ttggatttcg aattttgctc
     8221 attagaattt atttaggatt tattattttg aatttggtgc tttgcaagga ttggcgcttg
     8281 gaatgactgc tggctaataa atcaagtcat tcctctaagt gccttaattc tttcggtaat
     8341 agaagggtgg gttgaaaaaa gtccgcttaa gaacctcttt gttgtgccga acggattgtt
     8401 gatgtacaaa tgagccgtgg cgttattggc ccgccttaaa ggctggtgat aaactgatat
     8461 tttttccaga gccgaggcca ggccttcagg gtagcgggtc agcaatgaac cggaagcgtc
     8521 agccagatat tctctttttc tggaaaccgc cagctgaatc agtttggcga aaattggcga
     8581 cataatagcc aaaataattc caactatgat aataatcgct ccggcgccgc ctttgcggtc
     8641 gtcccggcct gaataaaaac taatccggat cagccagtct gacagcaaaa caactactcc
     8701 cagcaagaca atcactatca tcatcagtct gatgtcgtaa ttttgaatat gcgccagctc
     8761 gtgagcaatg acgccttcca gctcttcatt ttccagccgt tcaattaagc cggtcgtcag
     8821 ggcgactgaa gcgtgttttg gatcgcggcc ggtggcaaaa gcgttgggcg ccggatcatt
     8881 gataatatga actttgggaa ctggcaggcc ggcggtaatg gaaagattct caaccagccg
     8941 gtaaacgtaa ggattatcat cttttttgat ctctttggcg ccggcggcca gcaaagcgac
     9001 tttatcgccg gagaaataac tgaataaagt catcacgact gaaacaatca ccgcgataat
     9061 aattcccagc tgacccgaac cggtcagctc gccaaaaatc cagccgaggg caatcacaaa
     9121 tatggtgaaa agcaaaatta aaaaagttgt tttgcgtttg ttggaaagaa tctgttcgtg
     9181 catctatgta tgaaataggt tttggttata aggtttgggt atggcggcca gtatagtttt
     9241 agggcttggg ttttggtaaa aaagcctaaa cctaaaacca aagaccatac tagctgccac
     9301 acctaaaacc aaaaccataa ccatacgggc cgctaaaact aatcacgaaa accaattgaa
     9361 tttcggtatt aaaactttac ccttactgcc tctctttcct tttcatcctc aacttcaaaa
     9421 tattctttgg ccataaagcc gagcatccgg ccaatcatgt ttgttggaaa aacctgaagt
     9481 ttagtgttaa acaaccggac attattatta taaaatctcc gggcggcctg aattttattt
     9541 tccgtatcaa ccagttcatc ctgaagctgg ataaagtttt gagtggcttt taagtcagga
     9601 taattttcag cgaccgcgaa cagtgatttc aaagtgtccg acagcatatt ttccgctttt
     9661 gatttttcag ccattccctg agcctg
//
LOCUS       DASWHZ010000003         7382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_11664, whole genome shotgun sequence.
ACCESSION   DASWHZ010000003 DASWHZ010000000
VERSION     DASWHZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11664"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..769
                     /gene="cyaB"
                     /locus_tag="VGA49_00055"
     CDS             203..769
                     /gene="cyaB"
                     /locus_tag="VGA49_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEX9664204.1"
                     /translation="MANNYIEIELKFPLKNREKTIEVLNQQAELKADNIFQKASYYLP
                     AHRDFLAHQYPYEWLRLRESDNKFFLTYKHFYPENVLVTDYCDEFETEVKDLNTMKKI
                     FQVLDFKEIAVVEKRRSIWNYKEAEITVDQVEGLGDYLEAEALKHFTDPKEGKKYLMD
                     ILRELNLDLGEEELKGYPYLLLKKWGKI"
     gene            959..2290
                     /locus_tag="VGA49_00060"
     CDS             959..2290
                     /locus_tag="VGA49_00060"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9664205.1"
                     /translation="MDTQKILDQVQRDNVKFIQLQFTDILGVVKSLTIPAKHLEDSLK
                     YGTWFDGSSIEGFARIQESDMFLKPDVATYAVIPWLESADGQTARFICDVYKPDGAPF
                     EGDPRYILKKVLAEAREMGFNYNTGPELEFFLFKKEFGKIKSLPHDTGGYFDLTTDEA
                     YEVRRDMTVALERFGINVEASHHEVAVGQHEIDFRYDNALRTADSATTLKFVLKAVAQ
                     KHGLRVTFMPKPIAGINGSGMHVHQSLFTTDSNNNAFYDESDQYKLSRIAYNFIAGQL
                     EHIKAMSSIIAPTVNSYKRLVPGYEAPVYILWARMNRSALIRIPRVSEGKYQSTRCEL
                     RCPDPSCNLYLAFAVMLKAGLDGVKKNLMPPKPVERDVYHFDQNQLAEHNIKTLPHSL
                     WQALKELKEDEVIQSALGPHLFERYIEAKTKEWDEFRLNVSQWELDRYLEI"
     gene            2336..2977
                     /locus_tag="VGA49_00065"
     CDS             2336..2977
                     /locus_tag="VGA49_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9664206.1"
                     /translation="MKNKFLLFGGLLILFLATTGQNGCLRRSVEPAPLAEPAGQADQN
                     GVSQQVPAPGADLTVPETRVVDDGQGEESVGSADQPAPSADETEPAEEAPVPAPPPDL
                     SPEPAPEPAPEPAPEPEPQPQVKSFNMTARQWEFTPSTITVNQGDTVKLTIQSIDVTH
                     GFSIPQFGVNATLQPGKTINVEFVADKKGAFTYFCTVFCGSGHPDMKGTLVVQ"
     gene            3056..4261
                     /locus_tag="VGA49_00070"
     CDS             3056..4261
                     /locus_tag="VGA49_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664207.1"
                     /translation="MRGETMSPPGAEFGELKSIEDIRKGLKQVLADVFEKPRKPGEEK
                     KFNPVYESAKFTALSNALMAKEPTAEAKALELEIQQGTEQFFKKGIDGRLADAEELSN
                     KGYPDEAKLAGDTADHYLESYRNMLDPAVHTKKGEKDQLLQSYESALKKFEEARKGTD
                     KFLKKNRPDSVSKRIDIELKKLEDVRPAKAPDQIQQCQKWIDDLRNNYGYPDKQYQTI
                     KRGVEADPQGVEQSKNWEETRQRLERTEKAIKAQKDLDQLRDKLGFGAETGPRTGGIA
                     AEVKKAIEAGDSDLMTKIRDRLQEKRGRLDAVKPENQAALSEEIDTAVREFEEASDLT
                     DRITSELNKIEEGKEMSIDDKLKKIDEIKKMWNLMLPLTNDRRQARNKLLWKVERVEE
                     NLRRELLRI"
     gene            4258..5439
                     /locus_tag="VGA49_00075"
     CDS             4258..5439
                     /locus_tag="VGA49_00075"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013195320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9664208.1"
                     /translation="MKFTKEFLMVIIAIILALIAFVIWRTFRAERIINLPTANIPVAQ
                     APGPGDLDQGPSLGESPPVADDFDDAPIAANLATNLEIPWALDFLPDGKILFTERPGR
                     VRLIDPPTGLSPEPILTIAEVAHQGEGGLLGLTVHPNFSQNQFVYVYYTYLNDGQLNN
                     KVVRFQFINDALVNPTVIIENIPGGGNHDGGRIKFGPDGMLYITTGDAGISRLAQEVD
                     STAGKILRLNDDGSIPADNPFQNSPVYSYGHRNPQGLAWDSQGRLWATEHGSQARDEV
                     NLIEPGKNYGWPNITGDQTAPGFVSPVIDSGAVTWAPGGAVFAGDIMYYTGLRGQSLY
                     RFKLKSAGPEILFSRQFGRLRAVELGPDGFLYLLTSNRDGRGLPTTEDDQIIIINPEK
                     L"
     gene            5530..5694
                     /locus_tag="VGA49_00080"
     CDS             5530..5694
                     /locus_tag="VGA49_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664209.1"
                     /translation="MGHHNCGCHSHERQHDHDQPKEKKEHVCACGHDHGHEKKEDTVC
                     ACGHDHCQCC"
     gene            5703..6695
                     /locus_tag="VGA49_00085"
     CDS             5703..6695
                     /locus_tag="VGA49_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9664210.1"
                     /translation="MANKKNNKNKFNINDLIVIGAGAAGLSAAIYAGRYLMKTVVFSK
                     EFGGETATAGKIWNYPGFKSIDGYELVKKMKDQAESLGAKIINSEVIKIKKKNYCFFV
                     TASDKKEYVANSVILAVGAERRKLGLPNEKELTGRGVHYCVTCDGPLFSGKTVGMVGG
                     GDSSVKGVNLLGQYVKKIYLIVREKEIMAEPINYKQMKRLGSQVEVLLETEVKKLFGR
                     KKLEKIVLSKARSGKKELKLDGLFIEIGAVPDVELAQSLGVQLDDHGYIKTDNLMKTN
                     VDGVFAAGDTVNLFGRFKQDITAAAMGAVAATSAYEDYKEHGELCYLHMKPTEK"
     gene            6864..>7382
                     /locus_tag="VGA49_00090"
     CDS             6864..>7382
                     /locus_tag="VGA49_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9664211.1"
                     /translation="MDYSLAIPAFIAGLITFLAPCTLPLVPAYLGFISGVSIEDLGDP
                     EKIKKVRGKIFLNGLFFVIGFSAIFVMFGVAAGLIGTLFGAYRIWLARLGGIFVIIFG
                     LFMLNVVKLPFLSKEVKVRIPSIFKKGKILNSFILGTVFGFGWTPCVGPILGSVLLLA
                     SSSATAFQGALML"
BASE COUNT         2126 a         1515 c         1843 g         1898 t
ORIGIN      
        1 tttttttatt taaagagttt tatttattcg tcctttttga tggcaaaaag taccaaaaac
       61 cactggggta gccgattcgc ccgtgtgcta tttttataat cagtttttgt cgcgggctca
      121 aggctacccc agacccctct ggtgcataga gcaaaaaatg tagtttggct ctttttaagt
      181 tattcgttac aatataataa atatggccaa taattatatt gaaattgaac tgaaatttcc
      241 gttaaaaaat cgggagaaga cgattgaggt tttgaatcag caggcggaat taaaagccga
      301 taatattttt caaaaagcca gctactatct gccggcccac cgggattttt tggcgcatca
      361 atatccttat gaatggttaa gacttcggga gtcggacaat aaatttttcc tgacctataa
      421 acatttttat ccggaaaatg ttctggtgac tgattattgc gacgagtttg aaaccgaagt
      481 taaagatcta aacaccatga aaaaaatttt tcaagtcctt gatttcaaag aaattgccgt
      541 cgttgaaaaa agacggagta tttggaacta taaagaggcg gaaattaccg tggatcaggt
      601 tgaaggcctg ggcgattatc tggaagctga agcgctgaaa cattttaccg accctaagga
      661 aggcaaaaaa tatttaatgg atattttaag ggaattaaat ttggacctgg gagaagagga
      721 gttgaaaggc tatccttatc tgcttttgaa aaagtggggg aaaatataaa ttgctaaatt
      781 gttagaaacg gctgaccggg accgcggttt gatgaccgcg ttatacgtgg tttactttga
      841 aagaaaatgc tgatacagta aactactggc ggatgtgtaa aaattaaaat aaacaccaaa
      901 atcaaaatga gaaaaaacaa ctatcatttt gattttttaa tttttaactt atatttatat
      961 ggatactcaa aaaattttag accaggtcca gcgggataat gttaaattta tccagctgca
     1021 gttcactgac attttggggg tggtcaaaag tctgacgatt cctgccaaac atctggaaga
     1081 ctctttaaag tacggcacct ggtttgacgg ctcgtcaatt gaggggtttg cccggattca
     1141 ggaaagcgat atgtttttaa agccggatgt cgccacttac gccgttatcc cctggctgga
     1201 atcagccgac ggtcagaccg cccgcttcat ctgcgatgtt tataaacctg acggcgcgcc
     1261 ttttgaaggc gatccccgtt atattttgaa aaaagttctg gctgaagccc gggagatggg
     1321 gtttaattac aataccggac ctgaacttga atttttctta tttaaaaaag aatttggcaa
     1381 aatcaaaagt ttgcctcatg ataccggcgg ttattttgac ctgacgaccg acgaagctta
     1441 tgaagtccgg cgggacatga cagtcgccct ggagcgcttt ggaattaatg ttgaagcgtc
     1501 ccaccatgaa gtggctgttg gccagcatga aattgatttt cggtatgata atgctttgcg
     1561 gacggctgac agcgccacta ctttgaaatt tgttttaaag gccgtcgctc aaaagcatgg
     1621 tttgcgcgtg acatttatgc ctaagccaat tgccgggatt aatggttcag ggatgcacgt
     1681 ccatcagagc ttatttacga ctgacagcaa caataacgct ttttatgacg agagcgatca
     1741 gtataagctg tcccggatcg cttataattt tattgccggc cagctggaac atattaaggc
     1801 gatgtcttca attatcgccc cgaccgttaa ttcttacaaa agattagtgc ccggctatga
     1861 agcgccagtc tatattctct gggcccggat gaaccggtcg gctttgataa gaattccgcg
     1921 ggtgtcagag ggaaaatatc agtcaacccg ctgtgagctg cgctgtcctg acccgagctg
     1981 taatttgtat ctggcttttg ccgtgatgct caaggccggt ctggacggag ttaaaaagaa
     2041 tttaatgccg cctaagccgg tggagcggga tgtttatcat tttgatcaaa accagctggc
     2101 cgaacacaac attaaaactt tgccccattc attatggcag gctttgaaag agctgaaaga
     2161 ggacgaagtg attcagtcag ccctgggccc gcatttgttt gaacgctaca ttgaagccaa
     2221 gacaaaggag tgggatgaat tcagattaaa tgtttcccag tgggagctgg atagatattt
     2281 ggagatctag ctggttatgg taaaatgaaa atagtaatca ttaatttaaa tttttatgaa
     2341 aaataaattt ttattgtttg gcggattgtt gattttattt ttggcgacta ccggacagaa
     2401 cggctgtttg aggcggtcag ttgagccggc cccgctggca gagccggcgg gacaagctga
     2461 tcaaaatggc gtcagccagc aggttccggc cccgggggct gatttaactg tgcctgaaac
     2521 gcgggtagta gatgacggtc aaggggaaga atcagtcggg tcagccgatc aacctgcccc
     2581 gtcggctgac gagacagagc cggcggaaga ggctccagtt ccagcgccac caccagatct
     2641 atcacctgaa ccggctcctg aaccagcccc tgaaccggct cccgaaccag agcctcagcc
     2701 ccaggttaaa tcatttaata tgaccgcccg gcaatgggag tttaccccgt caaccattac
     2761 agtcaatcag ggggacacgg ttaaattaac cattcagagc attgatgtta ctcacggttt
     2821 ttctattccc cagtttggcg ttaacgccac cctccagccg gggaaaacaa tcaatgttga
     2881 attcgtggcc gacaaaaaag gcgctttcac ttatttctgc acagtttttt gcggctcggg
     2941 ccatcccgat atgaaaggga ctttagtcgt tcagtagaga acattagcat taagcattgg
     3001 tataattcgt acgccattcg cagattagca tcgcgcattg gttaaatata aatttatgag
     3061 aggcgaaaca atgtctcctc caggggctga attcggggaa ttaaaatcta tagaggatat
     3121 tagaaagggc cttaaacaag tactggccga tgtttttgaa aaacccagaa agcccggaga
     3181 agaaaaaaaa tttaatccag tttatgaatc cgcgaaattc accgccttat caaacgccct
     3241 aatggcaaaa gagccaactg ctgaagctaa ggcgcttgaa ctggaaattc agcaaggcac
     3301 agagcaattt tttaaaaaag gcattgatgg tcggctagcc gacgctgaag aactgtcaaa
     3361 taaggggtat cccgatgagg ccaaactagc cggcgacacc gcggatcatt atttggagag
     3421 ctataggaat atgttagatc cggccgttca taccaaaaaa ggcgaaaaag atcagctttt
     3481 gcaatcttat gaatcggctc taaaaaaatt tgaagaggcg cgaaagggaa ctgataaatt
     3541 tttgaaaaaa aacagaccgg acagcgtttc caaaagaatt gacattgaac tgaaaaaatt
     3601 ggaagatgtc aggccggcta aggcgcctga tcagattcag caatgccaga aatggattga
     3661 tgatcttagg aataattacg gctatcctga taagcaatat cagactataa aaagaggggt
     3721 tgaggccgac ccgcaaggtg ttgagcaaag taaaaattgg gaggagacaa ggcagcggct
     3781 ggaacggact gaaaaggcta tcaaagctca aaaggattta gaccaacttc gcgataagct
     3841 gggattcgga gccgagacag ggccccgaac cgggggtatc gcggccgaag ttaaaaaagc
     3901 aattgaggct ggagacagtg acttgatgac aaaaatcagg gatcgtttgc aggaaaagcg
     3961 cgggcggtta gacgccgtta aaccagaaaa tcaggctgct ttgtcggaag aaattgatac
     4021 ggctgtccgg gaatttgaag aggcctcgga cttaactgac agaattacca gtgaattaaa
     4081 caaaattgaa gaaggcaaag aaatgtccat agatgacaag ttgaaaaaaa ttgacgagat
     4141 taaaaaaatg tggaatttaa tgttgccttt gacaaatgac agaaggcagg caagaaataa
     4201 gctgctatgg aaggtagagc gggttgaaga aaatttgaga cgggaattat tgagaatatg
     4261 aaatttacca aagaattttt aatggtgatc attgccatta ttctggcctt aatcgctttt
     4321 gtcatctggc ggacgttccg ggctgaaaga atcattaatt tgccaaccgc caatatcccg
     4381 gttgcccagg cgccgggacc aggagattta gatcagggcc cctctttagg ggagtcgcct
     4441 cccgtggccg atgattttga cgacgccccg atcgccgcca acctggcgac taatctggaa
     4501 attccctggg ccctggattt tttgcctgac ggaaaaattt tgtttactga acggcccggc
     4561 cgggtcagac tgattgatcc gccgactggc ttaagcccgg aaccaatttt aacaatcgct
     4621 gaagtggccc accagggaga aggcggttta ttaggtttga ctgttcatcc aaactttagt
     4681 caaaaccagt ttgtttatgt ctattacact tacctaaatg acggccagct gaataataaa
     4741 gttgtccgtt ttcaatttat aaatgatgct ctggtcaacc cgactgtgat tattgaaaat
     4801 attcccgggg gcggcaatca tgacggcggc cggatcaagt ttggaccgga cggcatgctc
     4861 tacatcacca ccggcgacgc cggtatttcc cggttagccc aggaggttga ttcaaccgcc
     4921 ggtaaaatat tgcggctgaa tgatgacgga tcaattccgg ctgacaatcc ttttcaaaat
     4981 tccccggtct attcctacgg ccatcgcaat ccccagggac tggcctggga cagccagggc
     5041 cggttgtggg cgaccgaaca cggttcccag gccagagatg aagttaatct gattgagccg
     5101 ggaaaaaatt acggctggcc taacatcacc ggcgatcaaa ccgctccggg atttgtcagt
     5161 ccggtgattg acagcggggc agtcacctgg gcgccgggcg gggcggtttt cgcgggagac
     5221 atcatgtatt ataccggctt gagaggccag agcttgtatc gatttaagtt aaaatcagcc
     5281 ggcccggaaa ttttgttcag ccggcagttc ggccggttgc gggctgttga actgggcccg
     5341 gacggttttt tatatctgct gaccagtaat cgcgacggcc ggggcctgcc aacaactgag
     5401 gacgatcaga taattataat taatcctgaa aaactttaaa aaaactttca gccaaaggct
     5461 gatccgcctc cccggcctcg ctccgcgaag cggggcgggc tggcggaaaa ttagaaagga
     5521 actaaaaata tgggacatca taattgcggc tgtcatagtc acgagcgcca gcacgaccat
     5581 gaccagccca aagaaaagaa ggaacatgtc tgcgcctgcg gccacgacca cggccacgaa
     5641 aaaaaagaag acactgtctg cgcctgcggt catgatcact gccagtgctg ttaattagct
     5701 atatggctaa taaaaaaaac aataaaaata aattcaacat taatgatctg attgtcattg
     5761 gcgccggcgc cgccggcttg tcagccgcta tttacgccgg ccggtatttg atgaaaactg
     5821 ttgtgtttag caaagagttt ggcggagaaa ccgccacggc cggcaaaatc tggaactacc
     5881 ccggctttaa atcaattgac ggttatgaat tggtcaaaaa aatgaaagac caggccgaat
     5941 cgctgggcgc taaaattatt aattctgaag taatcaaaat taaaaagaag aactattgtt
     6001 tttttgtgac cgccagcgat aaaaaagaat atgtggctaa cagtgttatt ttagcggtcg
     6061 gtgccgaaag acggaaactg ggcttgccaa atgaaaagga actgaccggc cggggggttc
     6121 attattgcgt cacctgcgac ggcccgctgt tcagcggcaa aactgtcggc atggtcggcg
     6181 gcggcgattc gtcggttaag ggagttaatc ttcttggtca gtacgtcaaa aagatttatc
     6241 tgatcgtccg ggaaaaagaa attatggccg aaccgattaa ttataagcaa atgaaacggc
     6301 tggggagtca ggttgaggtt ttattagaaa ctgaagttaa aaaattattt ggccggaaga
     6361 aactggaaaa aattgttttg tccaaagcgc gcagtgggaa aaaggagctt aagctggacg
     6421 ggttgtttat tgaaattgga gccgtgcccg atgttgaatt ggctcaatcg ctgggcgttc
     6481 agcttgatga tcacggttat attaaaactg acaatttgat gaaaaccaat gttgacggcg
     6541 tttttgccgc cggcgacaca gtcaatcttt tcggccggtt caagcaggat atcacggccg
     6601 ccgccatggg cgccgtggcg gcaacttcgg cttatgaaga ttataaggag catggggagt
     6661 tatgctatct gcatatgaaa ccaaccgaaa agtaagaaaa aaatttcaaa tgacaaaatc
     6721 caaatgacaa atcaaatcca aaatctcccg atggcgaagc catcgaggat cctcgacaaa
     6781 gtcgggacaa aatccaaaaa ttttgacatt tagattttga tcttcaatgg acatttgatc
     6841 tttgaaattt ggatttacac aatatggact actccctagc catcccggcc ttcatcgcgg
     6901 gattaataac ttttctggcg ccctgcactt tgcctttagt gccggcttat ttgggtttta
     6961 tcagcggggt ctcaattgaa gatttgggtg atccggaaaa aataaaaaaa gtccgcggaa
     7021 aaatttttct gaacggactg ttttttgtga ttggcttcag cgctattttt gtgatgtttg
     7081 gcgtggccgc cggtttgatt ggaacattgt ttggagctta ccggatttgg ctggcccggc
     7141 tgggcggaat ttttgtgatt atctttgggc tgtttatgtt gaatgtggtt aaactgcctt
     7201 ttttgagtaa agaagttaaa gtcaggatcc cgtcaatttt taaaaaaggc aagattttaa
     7261 actcatttat cctggggaca gtttttggtt ttggctggac gccctgcgtc gggccgattt
     7321 taggttcagt cctattgttg gcttccagtt cagccaccgc tttccagggc gctttgatgc
     7381 tt
//
LOCUS       DASWHZ010000004         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_754652, whole genome shotgun sequence.
ACCESSION   DASWHZ010000004 DASWHZ010000000
VERSION     DASWHZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754652"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VGA49_00095"
     CDS             complement(<1..471)
                     /locus_tag="VGA49_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664212.1"
                     /translation="MKTSQRTYKLLVLLAISFVNGVSVALLAYILSSFLGGITGPKFV
                     GLFFTISSVATLLAIHYFSYFINRLGKFYSLIILLTFNILALIGLGLIGATGPALILY
                     VIYSVAATLVWVNLDVFIEYFSKDAETGRIRGANLTIMNLGWVFGPFLSGFLLER"
     gene            complement(475..>1894)
                     /gene="thrS"
                     /locus_tag="VGA49_00100"
     CDS             complement(475..>1894)
                     /gene="thrS"
                     /locus_tag="VGA49_00100"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9664213.1"
                     /translation="YKTGEFIDLCRGPHVNSTKEIKEFKLERLAGAYWRGDEKNKMLQ
                     RIYGLAFDTKKELADYLTMREEARKRDHRKLGPELDLFTFSELVGPGLPLWTPKGTIM
                     RDVLDDYVWQLRKKAGYQKVEIPHITKKDLYETSGHWEKFKDELFKINTREGYVFALK
                     PMNCPHHTQIYKHLPRSYRDLPQRYANTTMVYRDEQTGELSGLSRVRCITQDDAHVFC
                     RSGQVKEEFLKIWDIIDTFYQAIGFEKLKVRLSFHDPANFDKYLGTPVVWREAERNLR
                     TIAKAKKVKAEEAIGEAAFYGPKVDFMAKDSIGREWQVATIQLDMNMPERFDLTCVNE
                     KGKEERIVMIHAAIMGSLERFLSVIIEHFAGAFPVWLAPVQAAIASVGSAHQDFAQKL
                     ADQLAEAGFRVETDFTSETVSNKIRKAEHHKIPYMLVIGDKEAGGEPLAVRIRGRKKV
                     EIMTAEEFIARLKRDVVDKRLF"
BASE COUNT          535 a          501 c          374 g          484 t
ORIGIN      
        1 ccgctccagt aaaaatccgg acagaaaagg accaaagacc cagccaagat tcatgatcgt
       61 taaattggct cctctgatcc tcccggtttc agcgtctttg gaaaaatatt caatgaaaac
      121 atccagattc acccagacca aagtggccgc caccgaataa atcacgtata aaatcaaagc
      181 cgggccggtg gctccaatca gacccagccc aatcagcgcc agaatattaa aggtcagtaa
      241 aataatcagc gagtaaaatt tccccagccg gttgataaaa taagaaaaat aatgaatggc
      301 cagcaaagtg gccaccgaag aaatagtgaa gaataagccg acaaatttcg ggcctgttat
      361 cccgcccaaa aatgaagaca aaatataagc cagcagggcg acggaaacac cattgacaaa
      421 actaatcgcc agtaaaacca gcaatttata agttctttgg cttgttttca taatttaaaa
      481 taatctttta tccaccacgt cccgcttcag ccgggcgata aattcttcag ccgtcatgat
      541 ttccactttc ttcctgccgc ggattctgac ggctaacggt tccccgccgg cttctttatc
      601 gccaataacc agcatataag gaatcttgtg gtgttcagct tttctgattt tattggaaac
      661 agtttcgctg gtaaaatcag tttcaaccct aaaaccggct tcagccagct gatcagccag
      721 tttttgggcg aaatcctgat gggccgagcc aacactggca atggccgcct gaaccggggc
      781 cagccagacc ggaaaagcgc cggcaaaatg ctcaatgatt actgataaaa atctttccag
      841 cgaacccata atagcggcgt gaatcatcac aattctttct tctttcccct tttcattaac
      901 gcaggtcaaa tcaaaccgtt ccggcatatt catgtccagc tgaatcgtcg ccacctgcca
      961 ctcccggcca attgaatctt tggccataaa atcaacttta ggcccgtaaa aagccgcctc
     1021 gccaatcgct tcttcagctt taactttttt ggctttggcg atcgttcgta aattcctttc
     1081 ggcttcccgc cagacgacgg gcgttcccag atatttgtca aaattagccg gatcatggaa
     1141 cgacagcctg actttcagtt tttcaaaacc aatcgcttga taaaaagtat caataatatc
     1201 ccaaatcttt aaaaattctt ctttgacctg gcctgaccgg caaaaaacat gggcgtcatc
     1261 ctgggtaatg cagcgaacgc gggacaagcc ggacagctcg ccggtctgtt catcccgata
     1321 aaccatggtc gtattagcgt agcgttgggg caaatcacgg tagctccgcg gcaaatgctt
     1381 gtaaatctgg gtatggtggg gacagttcat cggcttcagc gcaaaaacat aaccctcccg
     1441 ggtatttatt ttaaacagct catctttaaa tttctcccag tggccactag tttcatataa
     1501 atcttttttg gtaatatggg gaatttccac cttttgataa ccggcttttt ttcttaactg
     1561 ccagacataa tcatccagaa cgtcacgcat tattgtccct ttgggagtcc ataaaggcaa
     1621 acccgggcca accagctcgg aaaaagtaaa caaatccagc tccggcccca gtttgcgatg
     1681 atcgcgtttc cgggcctcct cccgcattgt caaataatca gccagctctt ttttggtgtc
     1741 aaaagccagg ccgtagatcc gctgaagcat cttgtttttt tcatctcccc gccagtaagc
     1801 cccggccagc cgttccagct taaattcctt aatctcttta gtggaattaa catgcggccc
     1861 ccgacaaaga tcaataaact ccccggtttt ataa
//
LOCUS       DASWHZ010000005         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2975838, whole genome shotgun sequence.
ACCESSION   DASWHZ010000005 DASWHZ010000000
VERSION     DASWHZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975838"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VGA49_00105"
     CDS             <1..549
                     /locus_tag="VGA49_00105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191681.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX9664214.1"
                     /translation="DISDMEEVPEEMKAIYKTSFQVDPGAFVEVAARAQKWIDQAISR
                     NIYLSSREITDVMTVYMNAWKKGLKTTYYLHMKPRHQAEQSTTKVNKAKQLGRTGFNG
                     SGKGFGQSVPAPTVIAAKPQPQPVAPSGQQTVQQSASKITSQTQIPAEKELVAVKKAV
                     IRTTVNSCPVDPQERLLCDSCQ"
     gene            648..1676
                     /locus_tag="VGA49_00110"
     CDS             648..1676
                     /locus_tag="VGA49_00110"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX9664215.1"
                     /translation="MIIGSSVDRSLQLRPLKYQWAYDLYNQAVANTWFPHEIQLGEDL
                     ADWKKMTEEERHAVSFFISFFNPSELLVNRCLALGVYPYVTAPECHLYLAKQMWEEAN
                     HSVAFEYVVETFPIERENAFTAHITTPSMAAKKDFNLKFLQRMTEDSLDIETIEGKKD
                     FVRNLVATNIIMEGIWFYSGFMVGLSFRQRNLMRNFGSLLDWVVRDESLHLQFGINLI
                     LTILDESPELLEGDFGQEIHQMIVDSVELESVYNKDLLPNGILGLNADYVNKYVQYIA
                     DRRLEELGFQPHYNASNPAKWMSTANDVFELVNFFESTNTSYEVDVRADSNKKKDESA
                     VSSQQPTE"
     gene            1679..2158
                     /gene="msrA"
                     /locus_tag="VGA49_00115"
     CDS             1679..2158
                     /gene="msrA"
                     /locus_tag="VGA49_00115"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9664216.1"
                     /translation="MDQKKIDKAMFGAGCFWGVQKAFDQVEGVISTYVGYSGGQTKNP
                     TYEEVCTDQTGHVEVVLIEYDPDQVSYDQLLKVFWKVHDPTTTNRQGPDVGRQYRSVI
                     FYFTDRQKAAAEVAKDKLTRSGQYRDKIVTMIEPAGEFYKAEDYHQKYYLKNLHATC"
     gene            2145..3098
                     /locus_tag="VGA49_00120"
     CDS             2145..3098
                     /locus_tag="VGA49_00120"
                     /inference="COORDINATES: protein motif:HMM:NF039189.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HEX9664217.1"
                     /translation="MPLVKNKIKTSPKDFFLHLLNIVMLYISAAGFGRILFVMINYYW
                     PDPLEFYGFNDFNSIRWAIASIIVAWPVYLIGARSLNRDYQKNPEKKNLRVKLWLEYF
                     TMFGAAVIILVTLMTVIYKFLNGEITPRFILKTLTMLYIAGVIFYYYFSTVKIGFEKS
                     RSRIKVLVILVSLTILAAIILGFMTVGSPSTERLRRFDDKRLSDLQSVQAEVINFWQR
                     TRRLPADLNELGQAAFWRLPVDPQTYQPYDYQIIDDLSFELCAVFATDKQAGSEISER
                     VAPVPGLIGGGQRQNIYEQDYQAGRNCFSFMIKPELYPELK"
     gene            3495..3578
                     /locus_tag="VGA49_00125"
     tRNA            3495..3578
                     /locus_tag="VGA49_00125"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3529..3531,aa:Tyr,seq:gta)
     gene            3582..3655
                     /locus_tag="VGA49_00130"
     tRNA            3582..3655
                     /locus_tag="VGA49_00130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3616..3618,aa:Thr,seq:tgt)
     gene            3691..3846
                     /gene="rpmG"
                     /locus_tag="VGA49_00135"
     CDS             3691..3846
                     /gene="rpmG"
                     /locus_tag="VGA49_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9664218.1"
                     /translation="MSQDNLVKLECGNCHRINYFSHRNKKTVKERLLLKKFCKWCKAH
                     QEHKETK"
BASE COUNT         1142 a          787 c          924 g         1148 t
ORIGIN      
        1 gatatttcag atatggaaga agtaccggaa gaaatgaaag caatttataa aacctctttt
       61 caggttgacc cgggtgcttt tgtggaagtg gccgcccggg cccaaaaatg gattgatcag
      121 gccatcagtc ggaatatcta tttgtccagc cgggaaataa ccgatgtgat gacggtttat
      181 atgaatgcct ggaagaaggg attaaaaacc acttactacc tgcacatgaa accgcggcac
      241 caggctgaac agagcacgac taaagttaac aaagccaaac agcttggacg gactggtttt
      301 aacggctctg gtaaaggttt tggtcagtcg gttcccgcgc cgacagttat tgccgccaaa
      361 ccccagcctc agccggtggc gccatcggga cagcagacag tccaacaatc agcttccaaa
      421 ataacttcac aaacccagat accggctgaa aaagaattag tggccgttaa aaaagcggtt
      481 atcagaacaa ccgtcaatag ctgtccggtt gatccgcagg agcgtttatt gtgcgatagc
      541 tgccaataaa taaaagtcaa aagtaataag taaaaagtaa aaaatttgga aatattttat
      601 cttcaaaact ttttactttt gatttctaac ttttaactta aatagatatg ataataggtt
      661 catcagttga tagaagtttg cagcttcggc cgttaaagta ccagtgggct tatgatttgt
      721 ataatcaggc cgtggccaac acctggtttc cccatgaaat tcagctggga gaagatctgg
      781 ccgactggaa aaaaatgact gaagaagagc gccacgctgt ttcttttttc atcagctttt
      841 tcaatccgtc agagctgtta gttaaccgct gtctggctct gggcgtttac ccctatgtga
      901 ccgcgccgga atgccactta tatctggcca aacagatgtg ggaagaagcc aatcactcgg
      961 tcgcttttga atatgtcgtg gaaacttttc caattgagcg ggaaaacgct tttacggctc
     1021 atattaccac cccgtcaatg gccgctaaaa aagattttaa tctgaaattt cttcaaagaa
     1081 tgactgaaga cagtctggat attgaaacga ttgaaggcaa aaaagatttt gtccgcaatc
     1141 tggtggccac caacattatc atggaaggca tctggtttta cagcggtttt atggttggtt
     1201 tgagttttcg ccagcggaat ttaatgcgaa attttggttc attgctggac tgggtggttc
     1261 gggacgaaag tctgcacctg cagtttggga ttaatttgat tttaacaatt ttggatgaaa
     1321 gtccggaatt attggaaggc gattttggcc aggaaattca tcagatgatc gttgattcag
     1381 ttgagctgga aagcgtttat aataaagatt tgctacccaa cggtattttg gggctgaacg
     1441 ctgattatgt taataaatac gttcagtata ttgccgaccg ccggttggaa gaactcggtt
     1501 ttcagccgca ttacaacgct tccaacccgg ctaaatggat gagcacggcc aatgatgttt
     1561 ttgagctggt caatttcttt gaatcaacca ataccagcta cgaagttgat gtccgggcgg
     1621 atagtaataa gaaaaaagac gaatcagccg tcagcagtca acagccaaca gaatagatat
     1681 ggatcagaaa aaaattgaca aagccatgtt tggcgccggc tgtttttggg gcgttcaaaa
     1741 agcttttgat caggttgagg gtgtaatttc aacttatgtt ggttattccg gcggccagac
     1801 taaaaatcca acttatgagg aagtttgtac tgaccaaacc ggtcacgttg aggtggtttt
     1861 aattgaatac gatcccgatc aggtttctta tgatcagctt ttgaaagttt tttggaaagt
     1921 tcatgacccg actacaacca accgccaggg gccggatgtc ggccgtcagt accggtcagt
     1981 tattttttat tttactgacc ggcaaaaagc agccgctgaa gtcgccaaag acaaattaac
     2041 ccggtctggc caatatcggg ataaaattgt tacaatgata gaaccagccg gcgaatttta
     2101 caaggctgaa gactatcatc aaaaatatta cctgaaaaat ctccatgcca cttgttaaaa
     2161 ataaaataaa aacatcgccc aaagattttt ttctccatct gcttaatatt gtcatgcttt
     2221 atatttcagc cgccggtttt gggcggattt tgtttgtgat gatcaattat tactggccgg
     2281 atccgctgga gttttacggt tttaatgact ttaattccat ccgctgggcg atcgccagta
     2341 ttattgtggc ctggccggtt tatctgattg gcgcccggtc gcttaaccgg gattatcaaa
     2401 aaaatcctga aaagaaaaat ttacgggtca agctgtggct ggaatatttc acgatgtttg
     2461 gcgcggcagt gattattctg gtgactttga tgacagttat ttataaattt ctcaatggtg
     2521 aaatcacccc ccggtttatt ttaaaaactc tgacaatgct ttatatcgcc ggagtgattt
     2581 tttattatta tttttcaacc gttaaaattg gttttgaaaa aagccggtct cggattaaag
     2641 ttttggtaat tctggtcagt ctgactattc tggcggcaat aattttaggg tttatgacgg
     2701 ttggatcgcc atcaaccgag cggttgcggc ggtttgatga caagcgattg tctgatttac
     2761 agtctgtcca ggctgaagtt attaatttct ggcaaagaac caggcgtttg ccggctgatc
     2821 tgaacgagct tggtcaggcg gctttttggc ggctgccggt tgatcctcag acctatcagc
     2881 cgtatgatta tcagataatt gatgatttga gttttgagct ctgcgctgtt ttcgcgactg
     2941 ataaacaggc cgggtcagaa atttcagaaa gagtcgcccc ggttcccggc ctgattgggg
     3001 gcggtcagcg gcaaaatatt tatgaacaag attatcaggc cggccggaat tgcttcagct
     3061 ttatgatcaa gcctgaactt tatcctgaat taaaatgaat aaccccgccg tcggcggggt
     3121 ttattatctg cacgccgctt catccccgcg gcaagccgcg gggtattcgc gggcagataa
     3181 taaaaagatt gacttttggt taacaaagta ttataatata tgataatcta ataatgtgcc
     3241 ttaagcgcat ttttaagtta gaaaaaggta tcagaaagtg tatgtcgggg tcggtaccga
     3301 agcggtcaaa tcccgcctat ggcgggaccc cgccaagacc gcttcgcggt cgcctggcgg
     3361 ggcgggtagt caaaattatg tattacgtat atatttagtc tacaaagaag ttttggagac
     3421 taaaacagaa gcacgaaaaa gagaaaatca gattaaacgg tataaaggag gcagagcatt
     3481 taaaaaactt ataggggtcg gtaccgaagc ggtcaaacgg agctgactgt aaatcagctg
     3541 gttttatacc tacgggggtt cgaatccctc ccggcccatt tgccagcgta gctcagctgg
     3601 ttagagcaac tgttttgtaa acagtaggtc gtcggttcga atccgaccgc tggctattaa
     3661 tcaataacca ataactaaca accaatttct atgtcacagg ataatctggt aaaattagag
     3721 tgcggcaact gtcaccggat caactatttt tcccaccgga acaaaaagac agtcaaagaa
     3781 agactgctgc ttaaaaagtt ttgtaaatgg tgcaaagctc accaggaaca taaagaaacc
     3841 aaataattat gcgagcaatt aaaaatattg gcgacatttt cttgttattt cttttagttg
     3901 ttgccttcta ttttgtttat gttgttttta aaccatttat ctgggttatt ttagtttcag
     3961 ttattattgc cgttattttt tatccgctgt atgaaattat t
//
LOCUS       DASWHZ010000006         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2980926, whole genome shotgun sequence.
ACCESSION   DASWHZ010000006 DASWHZ010000000
VERSION     DASWHZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980926"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VGA49_00140"
     CDS             complement(<1..311)
                     /locus_tag="VGA49_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9664219.1"
                     /translation="MNKKQEQLETMRHSAAHLMAAAVQELYPAAKFGIGPVIENGFYY
                     DFDLPAVLIPDDFKKIEKKMKELQRKNLSYERQEMKLAEAINFFKKIGQDYKVELLND
                     L"
     gene            complement(466..1650)
                     /locus_tag="VGA49_00145"
     CDS             complement(466..1650)
                     /locus_tag="VGA49_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664220.1"
                     /translation="MPPKKKKTTKPDTSRAARPVVDKPINVYRKIAFTFVGIAVVLLV
                     VIFYYTFSNVKISVKSMEEETTIDFIIRIKEGGPTTGSQLAGHILDVTKTAQAEFESS
                     FSLTGEPAQATGMVRLMNDSDIDQPLVATTRLLSPDDVLFRIRESVVVPANGSIEAEA
                     YADQPGVQGEIEPTRFTIPGLNTSRQLEVYAISDQKMTGGTKAAYQVTAEDLDKAQIQ
                     MIDELTIQASNEFKSTIPADVQILEDKILTQVTKREADAEIGDSSEKFSLDLEVRIIT
                     AAANRTQLTNLVGDLFRNNIPADKTLVGLDAGDISYEIQNYDPVEKAVHVKASISGQV
                     SLRSTSPIFDKDKLIGLSGPEVKSYLENFTSVLGVEVDFSPFWVRTVPNMKDHIEIEI
                     IE"
     gene            complement(1697..>3227)
                     /locus_tag="VGA49_00150"
     CDS             complement(1697..>3227)
                     /locus_tag="VGA49_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9664221.1"
                     /translation="WIPDYIAGKHGKKKIKYLHPLLEPILKKTYGVAIYQEQVLRMAR
                     DLAGFTLGEADVLRKAVGKKIPKLLAEQKEKFIQGCLKNNISKKIAQEVFEFIEPFAG
                     YGFNRAHGASYAMISYQTAYMKANFSSEFMAALLTAEGGDTDRIAIEVEECRKMGIEV
                     LPPDINESFSTFTVVASTINTDQPRIRFGLNGIKNLGSNIIKAIIKERKERGQFSALE
                     NFLERVRHKDLNKKSLESLIKSGSLDNLGERNQLLVNLEQLLNFSKAGQKEKESGQKN
                     LFALTPAMAVSASLQLAPAAAVSKKEKLAWEKEFLGLYISDHPLKDLSQRLAALALPL
                     NKLKSQVNNSLVTIAGIITKVKKIITKNGQAMMFVQLEDVGGKAEVIVFPKILQDNSE
                     LWREDKIVLVDGKVSDKDGDAKILADSVREVTTDNIEQAGKEVCISIDYPVNHQLADR
                     LKNVFISHPGDYKVLINVIGRQTSKKIETDYKISCDYQIKEVLGEIVGPEKIKINGQG
                     A"
BASE COUNT          792 a          788 c          675 g          972 t
ORIGIN      
        1 ttcaaatcat ttaaaagttc aactttgtaa tcctgaccta tttttttaaa aaaattaatg
       61 gcttcagcca gcttcatctc ctgccgttca taggataaat ttttccgctg gagttctttc
      121 atcttttttt caattttttt aaaatcatcc gggatcaaaa ctgccggcaa atcaaaatca
      181 tagtaaaaac cattttcaat caccggtcca atgccaaatt tcgcggcggg ataaagctcc
      241 tgaacggccg cggccatgag atgagcggcg gaatggcgca ttgtttccag ttgttcttgt
      301 tttttgttca tatattatta aaaaggcaca aggcacgagg caacaggcac aagggttggt
      361 gtcgccttcc ccggctgctg ccgggttcgg cgacatatta ttagaaaatc tattgcctga
      421 tcccttttgc ctcgccttgt gccttgtgcc tgttgccttg tgcctttatt caatgatttc
      481 tatttcaata tgatccttca tgtttggaac tgtcctgacc cagaacggag aaaaatcaac
      541 ttcaactccc aaaacgctgg tgaaattttc cagataggat ttaacttccg gtccgcttaa
      601 gccaatcagc ttgtctttat caaaaatcgg gctggtggag cgaaggctga cctggccgct
      661 gattgacgcc ttgacatgga ccgctttttc aaccggatca taattctgaa tttcatagct
      721 aatatctccg gcatccagac caactaaagt tttatcggcc ggaatattat ttcggaaaag
      781 atcgccgact aaatttgtca gctgggtccg gttggccgcc gcggtgataa ttctgacttc
      841 cagatccaga ctaaattttt cagaactatc gccgatttca gcgtcagctt ctctttttgt
      901 aacctgcgtt aaaattttat cttctaaaat ctgaacatca gccggaatgg ttgatttgaa
      961 ttcattactg gcctggatag ttaactcgtc aatcatttgt atttgggctt tatccagatc
     1021 ttcagccgtt acctgatagg ccgctttagt cccaccggtc attttttggt cgctgatggc
     1081 gtaaacttcc agttggcggc tggtatttaa accgggaatg gtaaatcggg tcggctcaat
     1141 ttctccctga acccccggct ggtcagcgta agcttcagcc tcaatactgc cgttggccgg
     1201 gacaacaacg ctttccctga ttctgaataa aacatcgtca ggcgacaaca gcctggtggt
     1261 ggccaccagc ggctgatcaa tatcggaatc attcattaat ctgaccattc ctgtcgcctg
     1321 agccggctcg ccggtcaggc tgaaacttga ttcaaattct gcctgggcgg ttttggtcac
     1381 gtccagaatg tggccggcca gctgactgcc agtcgtcggc ccgccctctt ttatcctgat
     1441 gataaaatca attgtcgtct cttcctccat cgacttaacg gaaattttga cattggaaaa
     1501 agtgtaataa aaaataacca ccagcaaaac aacggcgata ccgacaaaag taaaagctat
     1561 tttgcggtaa acattgattg gtttatcaac caccggcctg gccgcccggc tggtgtctgg
     1621 tttggttgtt ttcttctttt ttggaggcat aggtttttat attataaaaa taacaaattt
     1681 attcaaaaaa aacaagtcag gccccctgac cgttaatttt tattttttcc gggccaacta
     1741 tctcccccag gacctcctta atctgataat cacagctgat tttatagtca gtttcaattt
     1801 tttttgaagt ttggcggccg atgacattta ttaaaacctt ataatcgccg ggatgactga
     1861 taaagacatt ttttaggcgg tctgccagct gatgattaac cgggtaatca atactaatgc
     1921 aaacttcttt accggcttgc tcaatgttat ccgtggtaac ctctctgacg ctatcagcca
     1981 aaatcttagc gtcgccgtct ttatcagaca ccttcccgtc caccaggaca attttatctt
     2041 cccgccacag ctctgaatta tcctgtaaaa ttttggggaa gacaatcact tcagccttgc
     2101 cgccaacatc ttccagctgg acaaacatca tcgcctggcc gtttttggta atgatttttt
     2161 taactttggt gattatcccg gcgattgtta ccagactgtt attgacctgg ctcttaagtt
     2221 tgttcagcgg cagagccagc gccgccagcc gctggcttag gtctttcagg ggatgatcag
     2281 aaatatacag tcccaaaaac tctttttccc aggccagttt ttcttttttg gaaaccgccg
     2341 cggccggagc cagttgcaga ctggcgctga cggccatggc cggagtcagg gcgaacaaat
     2401 tcttctggcc ggattctttt tctttctggc cggctttgga aaaattaagg agctgctcca
     2461 ggttaaccag cagttggttg cgttctccaa gattatccag actgcctgac ttgatcaggc
     2521 tttccagaga ttttttattc aaatctttat gccggacccg ctccagaaaa ttttccaggg
     2581 cgctgaactg gccgcgctct tttctttctt taataatcgc tttaataata ttgcttccta
     2641 aatttttaat gccgttcagg ccaaaacgga ttcggggctg atcagtatta atagtggaag
     2701 cgacaaccgt aaaagtgctg aaactttcat taatatccgg cggcaggact tcaattccca
     2761 ttttccggca ctcttccacc tcaatcgcga tccggtcggt atcgccgcct tcggccgtca
     2821 gcagggccgc catgaattct gaagaaaaat tagctttcat gtaagcggtc tgataagaaa
     2881 tcatggcgta gctggcgcca tgggcccggt taaaaccgta gccggcgaac ggctcaataa
     2941 actcaaagac ttcctgggcg atttttttgg aaatattatt tttcaggcaa ccctgaataa
     3001 atttttcttt ttgctcggcc agcagtttgg gaattttttt accaaccgct tttctcaaaa
     3061 catcggcctc ccccagtgta aagccggcca gatcgcgggc catccgcaaa acctgctcct
     3121 gataaatcgc cacgccataa gtctttttta aaatcggttc cagcaacgga tgcaaatatt
     3181 taattttctt tttaccgtgc ttgccggcga tgtaatcggg gatccat
//
LOCUS       DASWHZ010000007         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3352999, whole genome shotgun sequence.
ACCESSION   DASWHZ010000007 DASWHZ010000000
VERSION     DASWHZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3352999"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2062)
                     /locus_tag="VGA49_00155"
     CDS             complement(<1..2062)
                     /locus_tag="VGA49_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664222.1"
                     /translation="MEKARGHEVLSHKERQARIKELKSFVESYQGLREKQKEIRQELL
                     ETEIKDKAKQIFIKTFQQVDTHHRDEYCVNVGIEKRSALTEVTLDDWEQIERHYFNLA
                     REQLGDSGLEQLGEKTFDQQVRDKAWEIFDQKSDSDQRSILNEIEADRVLFARRIPEE
                     IRKVQDHPDLINQAKEEILAEQLPPIDPGTQEAAEFFDTSQKDSQLAKNRFWDKMRTE
                     LEEKVVSLADDHPDAQPELKAKINRNNFRLRKYFETSPDENKSIIGRYQKELVELEAL
                     EPKKQGKKQPFEILKTDLEKAEQTIGISARVAEKREEILKSRTEKILNPVLEQEAILR
                     KEIKDRVKEVYAAESRENKKEIHQKLGQRGYQANLPWILKKIEDQYLSKAVKIEVPKG
                     GDFAKEYKKYLASKTEEELKKFLVENFNNQPTDKQEAIAAEIGLKMLKKGEEKAWRTL
                     ENKLRREARNQLSSDDKDNQTKLKAKIREIFESADQDEKENYGRQVGEPEVARKSNEF
                     VKYGLESIYGQAAIGQQAVDLNDQEAIQIIINAHGTDYDKHRLGYLEQRGDVVVFIDQ
                     QAKEHLKKRVEEILKSLSDDERRNLLEQIGQAVEGKFDSLKDCINDQLVRKRDQEIRA
                     KARSKFESDKADQKDELMMKIGQEMIKRDWRAFWQVVEDNYDNEIKREDFTDYLRHNR
                     QLSLL"
     gene            complement(2080..3486)
                     /locus_tag="VGA49_00160"
     CDS             complement(2080..3486)
                     /locus_tag="VGA49_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664223.1"
                     /translation="MPRLDIQNLAKPEIITNQDLIGDLRNRLEKHIVFFNDEQEAGWF
                     YRNIGQLVEETPRLKNNYPDINQAYQSMIIKAGFIGLPLLSAENILDLMENNFTAIFE
                     VQKINEFYSVWEKVKTKLVGIIQYEDRDVFKKKIRETLLRNKEKITAQKITVQGTEVE
                     PTIGSWLKDYNAALGLGPVNRVKQAEYLTDSKNIKNVSLEEKDKLKMLFNFYEKLKYS
                     SMTPEGLEENVQAEIGRKPMVFREGELIEAYDYNTLQGLKGLVEAGLVPGVSKVEDLH
                     PAFVEALQRGKKTQDKKTKEAKIVKQTKSELVDKLKFKPAEEDALQAKVLDLTQATKK
                     DSSQLQKKLLIQINPPKAAQKPDKLEVLAILQILAETASLDRLLKDNKIQNLLISSLK
                     SAGKNNLMEGLKVFPEAPEYLSHLLQFILKEKLGMDNNQSAIYGLRLTNLMKQAGKKM
                     YSQLVYYDLETKQFAWKE"
     gene            complement(3509..>4526)
                     /locus_tag="VGA49_00165"
     CDS             complement(3509..>4526)
                     /locus_tag="VGA49_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664224.1"
                     /translation="NLQEERDALLEASDKKIDVAEADKIKLFDRTRATAGSEDLIRKD
                     GKWQDEKYMQGQIEKHETDYENFSQEAADQKRLSATLAPGTDFYARRNFRNAISEEVG
                     KLPRESMEATELSSNMQLALVDKNKSQFAALLMKATQDGNDNEVWKEFGFQGDRAGIE
                     EFRKEVLVKKLKMTGQESMSLLSDVGYINEKIGHTAASRLYTVEDGIYRPTTDEEQAA
                     AVTNENLKKDTENFLRHSNRLNLGSESPDGKLKIDLSGKLYIEQNQGKIIDLLRSKPD
                     RINPNLMKKLMQDIGSLQDMVDKKLIPQEMIGLIKRAAGQFEEGKTTGQILEERAKEI
                     SGKK"
BASE COUNT         1119 a         1054 c          724 g         1629 t
ORIGIN      
        1 caagcaacga cagctggcga ttatgcctta aataatccgt aaaatcttct ctttttattt
       61 cattatcata attatcttca acgacctgcc aaaaagcccg ccaatcccgc ttaatcattt
      121 cctggccgat tttcatcatc agttcgtctt tttgatcagc tttatcagat tcaaatttag
      181 atctggcttt agctctaatc tcctgatcac gctttcggac aagctgatca ttgatacaat
      241 cttttaaaga gtcaaactta ccctcaaccg cctggccgat ctgttccagc agattccggc
      301 gttcgtcatc tgataaactc tttaatattt cttcaactct ttttttcaga tgctctttgg
      361 cttgctggtc tataaagaca accacatctc ctctctgctc caaataaccc agcctgtgct
      421 tatcgtaatc tgttccatgg gcgttaataa taatttgaat ggcttcctga tcatttaaat
      481 caaccgcctg ctggccgata gccgcctgac cataaattga ttccaggccg tatttgacaa
      541 attcattact ttttcgggct acttcaggct ctccgacttg gcggccataa ttttcttttt
      601 cgtcttgatc ggcgctttca aaaatttccc tgatcttagc tttgagttta gtttgattat
      661 ccttgtcatc cgacgacagt tgattgcggg cctctcttct taatttgttt tccaaagtcc
      721 gccaagcttt ttcctcgcct tttttcagca ttttcaaacc aatttcggcg gcaatagctt
      781 cctgcttatc agtcggttga ttattaaaat tttcaactaa aaactttttt aattcttctt
      841 cagttttgga tgctaaatat tttttatatt ctttggcaaa atcgccgcct ttggggactt
      901 caatcttaac cgcttttgac aaatactgat cttcaatctt ttttaaaatc catggtaaat
      961 ttgcctgata accgcgctgg cctaattttt gatgaatctc ttttttattc tctcttgatt
     1021 cagcggcgta aacttctttg accctgtctt taatttcttt tcttaaaata gcttcttgtt
     1081 ccaaaactgg attaagaatt ttttcagtcc gactttttag aatctcctcc cgcttctccg
     1141 caacccgggc tgatatgcca atcgtctgtt cggctttttc caaatcagtt tttaaaattt
     1201 caaagggctg tttttttcct tgttttttgg gctctaaagc ttccagttca accagttctt
     1261 tttgatatcg accgattatc gacttattct catccgggct agtttcaaaa tatttgcgca
     1321 atctgaaatt atttctatta atcttggcct ttagttcagg ctgggcgtcc ggatgatcat
     1381 cagccaaact gacaaccttt tcttccagtt cagttctcat tttatcccaa aatctatttt
     1441 tggcgagctg actgtctttt tggctagtat caaaaaattc agcggcttcc tgggttcccg
     1501 gatcaatcgg cggcagctgt tcagccaaaa tttcttcttt ggcctgatta attaaatcgg
     1561 ggtgatcctg aacttttctg atttcttcag gaattctccg ggcaaataaa acacgatcgg
     1621 cttcaatttc gtttaaaatt gatctttgat ctgaatcact tttttgatcg aaaatttccc
     1681 aagccttgtc cctcacttgc tggtcaaaag ttttctcccc aagctgttcc aggccactgt
     1741 cccccagctg ttcccgcgcc agattaaaat aatgccgctc aatttgttcc cagtcatcaa
     1801 gagtaacttc ggtcaaagct gatctttttt ctatgccgac attaacacaa tattcgtccc
     1861 gatgatgggt atcaacttgc tgaaaagttt tgataaaaat ttgcttggct ttatctttaa
     1921 tttctgtttc taataattcc tgccgaatct ctttttgttt ctcccgtaaa ccctgatagc
     1981 tctcaacaaa tgatttaagc tctttaatcc tggcctgtct ttctttgtga cttaaaactt
     2041 catgacctct agctttttcc atattagcca taatgttgat tactctttcc aggcaaactg
     2101 tttagtctct aaatcgtaat aaaccagctg tgaatacatt tttttgccgg cctgtttcat
     2161 caaattagtc agtcgcaagc cataaatcgc cgattgatta ttatccattc ccagtttttc
     2221 ttttaaaata aattgtaata aatgacttaa atattccggg gcttcgggaa aaaccttcaa
     2281 tccttccatt aaattatttt ttccggctga ttttaaagaa gatataagta aattttgaat
     2341 tttattatct ttaagcagcc tgtccaggct ggcagtttcc gctaaaatct ggagaatagc
     2401 caaaacctcc agtttgtcag gtttttgagc agctttggga ggattgatct gtataagaag
     2461 ttttttttgc aattgactgg aatccttttt ggtggcctgg gtcaagtcaa ggactttagc
     2521 ctggagggcg tcttcttccg ccggcttaaa ttttagcttg tcaaccagct ctgattttgt
     2581 ttgcttaacg attttagctt ctttcgtttt tttatcttga gtttttttgc cgcgctgaag
     2641 tgcttcaaca aaagctggat gcagatcctc aactttactg actcctggca ccagaccagc
     2701 ttcaaccagg ccttttaagc cctgtaaagt attgtagtca taggcttcta ttaattctcc
     2761 ctctctaaat accatgggct tgcggccaat ttccgcctga acgttctctt ccagcccttc
     2821 gggcgtcatt gaagaatatt tcagtttttc ataaaaatta aacaacattt tcagtttatc
     2881 tttttcttcc agggaaacat ttttaatatt tttactgtca gtcagatatt ccgcctgctt
     2941 gacccgatta accggcccta aacccaaagc ggcgttataa tctttgagcc agctgccaat
     3001 cgtcggttca acttcagtcc cctgaacagt aattttttgg gcggtaattt tttctttgtt
     3061 tctcaaaaga gtttcccgga ttttcttttt aaaaacatcc cgatcttcgt attgaataat
     3121 gccaaccagc ttggtcttga ccttttccca gactgaataa aactcattaa ttttttggac
     3181 ttcaaaaatt gccgtaaaat tattttccat caaatccaaa atgttttctg ctgataataa
     3241 gggcaaacca ataaaaccgg ctttgataat cattgactga taagcctgat taatatcagg
     3301 ataattattt ttcaaacgag gagtttcctc aaccagctga ccaatattcc gataaaacca
     3361 gccggcttcc tgctcatcat taaaaaagac aatatgtttt tccagccgat ttcgcagatc
     3421 accaatcaaa tcctgattag taataatctc cggcttggct aaattttgaa tatctaatct
     3481 tggcataaat atttttaaaa tttgtcaatt atttcttgcc gctaatctct ttagctcttt
     3541 cttctaaaat ttgaccggtt gttttccctt cttcaaattg accagctgct ctttttatca
     3601 ggccaatcat ttcctgagga atcaattttt tatcaaccat atcttgtaaa gatccgatat
     3661 cctgcatgag ttttttcatc aaattcgggt tgatcctgtc aggtttgctt ctcagcaaat
     3721 ctataatttt cccctggttt tgttcaatat ataatttacc gctcaaatct attttcaatt
     3781 taccatcagg tgattcactg ccaagattta accggttgga atgacgtaaa aaattttcgg
     3841 tgtctttttt taaattttca ttagtgaccg ccgccgcctg ttcttcatca gttgtcggcc
     3901 gatagattcc atcctccacc gtgtacagcc gggaagcggc cgtatgaccg attttttcat
     3961 taatatagcc aacatcactt aacaaagaca ttgattcctg accagtcatt tttagttttt
     4021 taaccaaaac ttcttttctg aactcctcaa tgccggcccg gtcgccctga aacccaaact
     4081 ctttccagac ttcgttatca ttaccatcct gggtcgcttt cattaataac gccgcaaact
     4141 ggcttttatt tttatcaacc agggccagct gcatgttgga cgacaattcg gtcgcttcca
     4201 ttgactcccg gggcagtttg ccaacttctt cagaaatcgc gttacggaaa ttccgacggg
     4261 cgtaaaaatc cgttccagga gccaaagtag ccgacaatct tttctggtct gccgcctcct
     4321 gactaaaatt ttcatagtca gtctcatgtt tttcaatctg cccctgcata tatttttcgt
     4381 cttgccattt accgtctttt cggatcaaat cttctgaacc agctgtggct cttgtcctgt
     4441 caaataactt aatcttatct gcttcagcta catcaatctt tttgtcagaa gcttccagta
     4501 aagcatctcg ttcctcttga agattt
//
LOCUS       DASWHZ010000008         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2256439, whole genome shotgun sequence.
ACCESSION   DASWHZ010000008 DASWHZ010000000
VERSION     DASWHZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256439"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGA49_00170"
     CDS             complement(<1..855)
                     /locus_tag="VGA49_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9664225.1"
                     /translation="MPKAKKSGSSRVKKQKIKKTKSKKQVVKKKSVRKKPAKKPAKKI
                     KKVRTRKAARPKKAEIELFVSEKLAALLKAGRQRSFVTVNELLYAFPEVEEYIEDYEE
                     FLKDLENYGVQIVEIKNILEKKEDRQKTLEQLGLIKGKDKRRFDLGDISADSIQMYLR
                     EIGKVPLLKGEEEVALAKRKEKGDEEAKNRLIQANLRLVVSIAKKFTGKTLSLLDLIQ
                     EGNIGLFRAVEKFEYRKGYKFSTYATWWIRQAITRALADQSRTIRIPVHMVETINKFS
                     KIERRLIQD"
     gene            complement(868..2664)
                     /gene="dnaG"
                     /locus_tag="VGA49_00175"
     CDS             complement(868..2664)
                     /gene="dnaG"
                     /locus_tag="VGA49_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9664226.1"
                     /translation="MSQTEEIKSKLDIIDIISEYINLKPAGSNFKALCPFHNEKSPSF
                     MVSREKQIWHCFGCSAGGDIFSFVQEYEGVEFPEALRILANKAGVTLTKTNPQLHNQK
                     TSLYDVCQTAANFFHQLLLKHPKAQKALDYLKSRGVNQEFVKNFQLGYSPDAWDVLYK
                     YLKEKKFDDQQIFLAGLTVKKDREYGYYDRFRNRLIFPIRDLHKNVVGFTARALDEER
                     DKMGKYINTPQTLIYNKSQVIYGLDLAKADIKKENLTVMVEGQMDVIASHQAGVKNVV
                     ATSGSALTNEQVGFLKRYSNNIALAFDADLAGEAAAKRGIDVALTQGMNIKVITIPHG
                     KDPDECIGENVDDWKQAIKNSSRYLDYYFKKVFEAFDVTNPESKTKAAKYILRELAKV
                     QDKVEQTHYLQLLSEKINVSETILRETLNKSTKPQNIKTLKHENIKTSQTNNKGRFQD
                     RNSLLSQRLLSLMLKYPEHINYVSDHLEPQMLVEGWNDLYKNIIIYYTNKTENQQDFS
                     YFLAKAGSNLVDEADRLVLLADKDYSSFDGSQIKKEIVAIAGQLKKNNILKQLKELET
                     RIKEMEKTADQSAIGSLTEEFDLLTQQLKSFA"
BASE COUNT          747 a          590 c          506 g          954 t
ORIGIN      
        1 atcctgaatc agccgccgct ctatttttga aaatttattg atggtttcca ccatgtgaac
       61 cgggattctg attgtccggg actggtcagc cagcgcccgg gtgatggcct ggcggatcca
      121 ccaggtagcg taagtggaaa atttatagcc tttgcggtat tcaaattttt ccaccgcccg
      181 gaacaaacca atattgcctt cctggatcaa atccagcaga gacaaagttt tgcctgtaaa
      241 ttttttggcg attgaaacca ccagccgtaa attagcctga attaatctgt ttttggcttc
      301 ttcatcgcct ttttcttttc ttttggccaa agcgacctct tcctcgcctt tcaggagcgg
      361 cactttgccg atttcccgta aatacatctg aatggagtca gccgaaatat ctcccaggtc
      421 aaaccggcgc ttgtctttgc ctttgatcag ccccagctgt tccaaagttt tttgccgatc
      481 ttcttttttt tccagaatat ttttaatttc aacaatttgg actccataat tttccagatc
      541 ttttaaaaat tcttcgtaat cctcaatata ttcttccact tccggaaaag cgtacaataa
      601 ttcattaact gtcacaaaag accgctgacg acctgctttc agcagagccg ccaatttttc
      661 agaaacaaat aattcaatct cggccttttt gggcctggcc gctttcctgg tcctgacttt
      721 tttaattttt ttagctggct tttttgcagg ttttttcctg actgatttct tcttgacaac
      781 ctgcttttta gattttgttt ttttgatttt ttgttttttc acacggctac tgccggattt
      841 cttagctttg ggcataattt aaaaaaatta agcgaagctt ttaagttgtt gggttaataa
      901 atcaaattct tcagtcaagc ttccgatggc cgactgatca gcggtttttt ccatttcctt
      961 aatcctggtc tcaagctctt tcagttgttt taaaatatta tttttcttca gctggccggc
     1021 gatggccact atttcttttt tgatctgact gccgtcaaaa ctgctgtaat ccttgtcagc
     1081 cagcaaaacc aaacggtcag cctcatcaac taaatttgag ccagccttag ccaaaaaata
     1141 agaaaagtcc tgctgatttt ccgttttgtt agtgtaataa attattatgt ttttgtaaag
     1201 gtcattccag ccttcaacca gcatttgggg ctcaagatga tcgctgacat aattgatatg
     1261 ctcggggtat ttcaacatta aagataaaag tctttgagac aataaactgt tcctgtcctg
     1321 aaaccggccc ttattgtttg tttgagatgt tttaatgttt tcatgtttta atgtttttat
     1381 gttttgtggt tttgttgatt tattcaacgt ttcgcgcaaa atagtttcag aaacattaat
     1441 tttttctgaa agcaattgca gataatgagt ttgttcaact ttatcctgaa ctttggccag
     1501 ctcgcgtaaa atatatttag cggcctttgt cttggattcg ggattggtaa cgtcaaaagc
     1561 ttcaaaaact tttttaaaat aataatccag ataacgactg gaatttttaa tggcctgctt
     1621 ccagtcgtca acattttctc caatgcattc atcaggatct ttgccatgag ggattgtaat
     1681 cactttaata ttcatcccct gggtcaaagc gacatcaatc cctcttttgg cggccgcctc
     1741 gccggccaaa tcagcgtcaa aggccagagc aatattattg gaatatcttt ttaaaaagcc
     1801 gacctgttca ttagttaagg ctgatccgga agtcgccacg acatttttaa cgccggcctg
     1861 atgggaagca atcacatcca tctggccctc aaccatcact gtcagatttt cttttttaat
     1921 gtcagctttg gccaggtcca gtccgtaaat cacctggctc ttattgtaaa tcaaagtttg
     1981 gggcgtgtta atgtatttgc ccattttatc tctttcttca tccagcgccc gggccgtaaa
     2041 gccaacgaca tttttatgaa gatcgcgaat tggaaaaatt aaacgatttc taaaacggtc
     2101 ataatagccg tactcgcggt cttttttaac cgtcagacca gccaaaaaaa tctgctggtc
     2161 atcaaatttt ttttctttta aatatttata caaaacatcc caggcgtccg ggctataacc
     2221 taactgaaaa tttttaacaa actcctggtt aactccccgg ctttttaaat aatccagagc
     2281 tttttgagct tttggatgtt ttaataataa ttgatgaaaa aaattggcgg cagtctgaca
     2341 gacatcatat aaagaagttt tttgattatg aagctgggga ttggtttttg tcaaagtcac
     2401 gccggcttta ttggccagaa ttctcaaagc ctcggggaat tcaacgcctt cgtattcctg
     2461 aacaaaacta aaaatatcac ctcccgcgga acagccaaaa cagtgccaga tctgcttctc
     2521 ccgggaaacc atgaatgatg gtgatttttc attatggaac gggcagagcg ctttaaaatt
     2581 agatccggcc ggtttcagat taatgtactc ggagatgata tcaatgatat ccagttttga
     2641 tttgatttct tctgtttgtg acataaaaac aactatttaa tgtgccacaa taataccaaa
     2701 ttatactatt tttgaaaagg acttatttaa aaatgtcaac aggaagttaa aatgtcatag
     2761 tttcaagtat tttaaaatgt cataccaggt tcttttt
//
LOCUS       DASWHZ010000009         4843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1891405, whole genome shotgun sequence.
ACCESSION   DASWHZ010000009 DASWHZ010000000
VERSION     DASWHZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891405"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="rpsC"
                     /locus_tag="VGA49_00180"
     CDS             complement(<1..574)
                     /gene="rpsC"
                     /locus_tag="VGA49_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9664227.1"
                     /translation="MGQKVNPKSFRMGGKGLFTWDSKWFSKKNFPGLLKQDVKIRKFI
                     MKKLKTALVSKVEIERSRGNLAINIYAVKPGLIIGRAGAGIDELRSDIQTKILKDKKI
                     LLNINIKEVKKPSLTAQVVLQNMIFDLEKRLPFRRVLKQTIARVEKEGAEGVKVSVSG
                     RLNGAEIARSETLTSGKIPLHTLRANIDYAR"
     gene            complement(868..1200)
                     /gene="rplV"
                     /locus_tag="VGA49_00185"
                     /pseudo
     CDS             complement(868..1200)
                     /gene="rplV"
                     /locus_tag="VGA49_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362527.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(1206..1496)
                     /gene="rpsS"
                     /locus_tag="VGA49_00190"
     CDS             complement(1206..1496)
                     /gene="rpsS"
                     /locus_tag="VGA49_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX9664228.1"
                     /translation="MSRSLKKGPYVDEKLLKKMSKVKRGEKTIIKTWSRNSTITPEMV
                     GYTFGVHNGKTHLPVYVVENMVGHKLGEFSPTRKFVSHGGKIAKAQKEEAAK"
     gene            complement(1506..2333)
                     /gene="rplB"
                     /locus_tag="VGA49_00195"
                     /pseudo
     CDS             complement(1506..2333)
                     /gene="rplB"
                     /locus_tag="VGA49_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            complement(2330..2809)
                     /gene="rplW"
                     /locus_tag="VGA49_00200"
     CDS             complement(2330..2809)
                     /gene="rplW"
                     /locus_tag="VGA49_00200"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9664229.1"
                     /translation="MGIFDKFKKNKAASPAVKDSGISSQQKPAADKKEAGPKKEVNKV
                     VKERAEKPDKKTVKKVSKEDTGQAYRVLIKPVMTEKSTFLGEQSKYVFEVSQRANKIM
                     VKQAIKAVYGVDPVKVNITKVKGKQIRVGRRTGKRKDWKKAVVNLKKGQKIEVFEAI"
     gene            complement(2812..3444)
                     /gene="rplD"
                     /locus_tag="VGA49_00205"
     CDS             complement(2812..3444)
                     /gene="rplD"
                     /locus_tag="VGA49_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9664230.1"
                     /translation="MIKAKVYNLEGQVTGEKELNPKFFEVKTNQDLIHQVVVAQQANS
                     RQTFAHAKGRADVRGGGRKPWRQKGTGRARHGSIRSPIWRGGGVIFGPTKEKVFAKKI
                     NKKMKRKALLMCLSDRAFNERLILIDDLRLPEIKTRQMAGILNKLPLKNKTVLVALSD
                     SDQKVVKSTRNLSQAKTIRADSLNVMDVLKHEYLLMPVEAVEVVEKTYKL"
     gene            complement(3656..4267)
                     /gene="rplC"
                     /locus_tag="VGA49_00210"
                     /pseudo
     CDS             complement(3656..4267)
                     /gene="rplC"
                     /locus_tag="VGA49_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            complement(4469..4825)
                     /gene="rpsJ"
                     /locus_tag="VGA49_00215"
     CDS             complement(4469..4825)
                     /gene="rpsJ"
                     /locus_tag="VGA49_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX9664231.1"
                     /translation="MATKTEAKLNKGNNEEEARTRIRIKIRAYDHKIIDQSTKTIIET
                     AERSGASIAGPIPLPTEKTKFTVNKSTFVHKDSRDQYEMRVHKRLIDIYDPTPKTVDS
                     LMSLNLPAGVDVEIKM"
BASE COUNT         1139 a         1208 c          935 g         1561 t
ORIGIN      
        1 cccgggcgta atcaatatta gcccgaagag tatgcaatgg aatcttgccg gaagttaaag
       61 tttcgctccg ggcgatttcc gcgccgttca gccggccgga aacactgact ttaacgcctt
      121 cagcgccctc tttttccacc cgggcgattg tctgcttcaa aacccgcctg aatggaagcc
      181 ttttttccag atcaaaaatc atattctgca gaacaacctg ggcggttaaa gacggttttt
      241 ttacttcttt aatattaata tttaaaagaa tttttttgtc ttttaaaatc ttagtctgaa
      301 tatctgaccg caattcatca atcccggctc cggcccggcc aataattaac ccgggcttga
      361 cggcgtaaat attaatggcc agattacccc gtgaacgctc aatctccact ttggaaacca
      421 gggccgtctt taattttttc atgataaact tacggatttt gacatcctgc tttaaaaggc
      481 cggggaaatt tttcttggaa aaccatttgg aatcccaggt aaataatccc ttgccgccca
      541 ttctgaaact ttttggattg actttttgtc ccatacgcgt tagcctgctt tacgtttaaa
      601 tatttttttc aaaaatcctt ttttatcccc tggttttttc atctcttttt tatcctgatg
      661 ctccagctga cgatgtttgc cctgacggcg gtcatcaaac ggcgctggct ggtgatcctc
      721 ggccggcttt tccttaattt cttcagccgg ctgcttttgt tccagactta ctgttttagc
      781 cggctggact ttctcaaccg gctgcctggt tgaaaccatg gctggttcgg ccttttttgt
      841 ggctttttta acggccggtt tggttggctt aatttcaccc aggataatgt ttatatgaga
      901 tgatttcttg cgaatcggcg tcgcccggcc aaaagcccgc ggccgccagc gcatcaaagt
      961 cggcccctga tcaacagtga ttgttttgac aaataaatta tcccgttcca gcttaaaatt
     1021 attttcagcg ttggcaatcg ccgaatttaa aagctttaac aaaggccgag agcttttctt
     1081 agaactgaac tccaactgat gaccggcttt agtcactgac aaaccacgaa tcacgtcagc
     1141 cactaacctg acctttttag gcgacattct taaaaatcgt aatttggctt taacttccat
     1201 ataatttatt tagcggcttc ctccttttgg gccttggcaa ttttaccgcc atgagaaaca
     1261 aacttccggg tcggtgaaaa ttcacccagt ttatgaccga ccatattttc aacgacataa
     1321 accggcagat gcgtcttgcc gttatgcacg ccaaaagtat aaccaaccat ttccggagtg
     1381 atcgtggaat ttcttgacca tgttttaatg atagtttttt cgcctctttt aaccttagac
     1441 atctttttca aaagcttctc atcaacatag ggaccttttt tgagtgatct tgacatagac
     1501 ttagtttatt tcttccgccg tttaataata agtttatcag agtgtttctt ttgctttctg
     1561 gttctaacgc cataagcttt tttaccccag ggcgttttag gcgatttcag accaattggc
     1621 gcgtggcctt cgccgccacc atgaggatga tcaaccggat tcatttattt aacgccaact
     1681 gacggcctaa aacccaaatg tcttttccgg ccggctttgc ccagccggat cagacgatga
     1741 tcgctaaaac tgacccggcc gatagtggcc attgaatctt tgggaacctt tctgatttct
     1801 ttggaaggca aacgtaaagt ggcgtatttc ccctcaactg ccattaactg ggcggccatg
     1861 ccggctgacc ggactaaacc gccgccttta ccgggcctta attcaatatt aaaaaccata
     1921 gtgccggttg ggatatttgt cagtttcaaa cgattgccgg ttttaatatc aatctttgac
     1981 tcggatgaca gcacctggtc attgacaacc aaatcttccg gcgccaaaat atatcttttt
     2041 tcaccatcaa gataactaat cagggcgatc cgggctgacc ggttaggatc gtattcaata
     2101 gctgtcactt taccgggaac atcaaattta tcacgcttaa aatcaaccag ccggtaatac
     2161 cgcttggcgc cgccgccgcg atgacgaaca gtcacccggc ctttgctccg gccggctttt
     2221 cttttcctaa tgaccagcaa ttttttctcg ggatttttct tagtcagttc ttcccctttg
     2281 tcaacgctgg cgcctcgcct gccgggagtt gttggttggt atttcttaat catattgcct
     2341 caaagacttc aattttttgg ccctttttta aattaaccac ggctttcttc caatcctttc
     2401 ttttcccggt ccggcggcca actctgatct gcttaccttt gactttggta atattcactt
     2461 tgaccgggtc aacgccgtaa acagctttaa tagcctgttt aaccatgatt ttgttggccc
     2521 gctggctgac ttcaaaaaca tacttggact gctcgcctaa aaaggttgat ttttcagtca
     2581 taaccggttt aatcaatacc cggtaagcct ggccggtatc ttctttgctg acctttttaa
     2641 cggttttttt gtcaggtttt tcagcccgct ccttaacaac cttattgacc tctttcttag
     2701 gtccagcctc tttcttatcc gccgccggct tttgctgact gcttatcccg ctgtctttga
     2761 cagccgggct ggccgcttta ttctttttaa atttatcaaa aattcccata attataattt
     2821 ataagttttt tcaacaacct caaccgcttc caccggcatt aataaatatt catgtttcaa
     2881 aacatccatt acattcaagc tgtcagctct gatagtttta gcctgtgaca gattgcgggt
     2941 tgatttgaca accttttgat cgctgtcaga caaagcgacc agaacagttt tgttttttaa
     3001 aggcaattta ttcaaaatac cggccatttg tctggttttg atttccggca gccgcaaatc
     3061 atcaatcaga atcagccgtt cattaaacgc ccggtcggac aaacacatca gtaaagcttt
     3121 ccgtttcatt tttttattga tctttttggc aaaaactttt tctttggttg gaccaaaaat
     3181 cactcccccg ccccgccaga tcggtgatct gatagaacca tgccgggccc ggccggtgcc
     3241 tttctggcgc cagggcttgc ggccgccgcc tctgacatca gcccggcctt tggcgtgagc
     3301 aaatgtttga cgactgttgg cctgctgggc gacaaccacc tggtgaatca aatcctgatt
     3361 agtcttcact tcaaaaaatt tcggatttaa ttccttttct ccggtcacct gaccttctaa
     3421 attgtaaact ttggccttaa tcataaattt atttctcgtc ggctttaggc tcttcggtag
     3481 attcagccgg ttgttctttg tcagccaaaa cctcctcagg atctttaact gcttccggaa
     3541 ctggttcgac tggcgcctct tctttaactg atgctgtccc tcctgtttct tttgtcgcca
     3601 ttttaactgg agttgccttt tccaaagttt ctttcagttc catctcgcct tcagccttaa
     3661 tgaacagcaa actatttcgg tgtccaggca aagcgccttt gatataaagt aaattctttt
     3721 ccggatcaac ctctactatt tccaaattct taatggtcac gctctgattg cccatccggc
     3781 cgcccattct gacaccttta aaaacccggg cggcgtcagt cgctccaatt gagcccggca
     3841 tccggagctg atctttatgc ccatggctgg ccgggccgcc tttaaacccg tggcgcttga
     3901 cgacgccggc aaaaccacgg cctttggaaa cgccggtgac ggtgacctga tcgcctttga
     3961 caaaaacatc ggctgtaata tactgaccgg tcttaaattt tgaaacatct ttcagtctga
     4021 attccttcaa aaatcgcggg tgagcctggc cggttttaac gatttttttc agatggccag
     4081 ccagcggctt attaaccttt tttctgacgt caaaaccaac ctggaccgct gaatagctgt
     4141 ctttgtcaga ttttttgacc tgagtcacca aacagggacc ggcttcaatc atggttaccg
     4201 gcaccgaccg gccgtcctcc tggaaaatct gggtcatctg aatttttttg cctaaaacaa
     4261 atttcatgtc atttaaaaat aaaaaccaga agttttccaa aaaaataatt tttttaaaaa
     4321 acttccagcc ctattaattt tcaaaattat tcagactgat ttaataggtt cttattttgc
     4381 cgtctttaat ctccaaataa ccgttaagct tataaaggtt ttttctatta atgaaaatag
     4441 aaatcttctt tatcttccta agggttacct acattttaat ctcaacgtca actccggccg
     4501 gcaggttgag actcatcagc gagtcaactg tcttgggagt cgggtcgtaa atatcaatta
     4561 accgtttatg gacccgcatc tcatactgat cgcgggaatc tttatgaaca aaggttgatt
     4621 tgttaaccgt aaacttagtc ttttctgtcg gcagcggaat tggtccggca atagaagcgc
     4681 cgcttctttc agcagtttca ataattgttt tggttgactg gtcaataatt ttatgatcgt
     4741 aagctctgat tttaattcta attctggtcc gagcttcctc ttcgttattg cctttattca
     4801 gttttgcttc tgttttggta gccatcctgg gctaagttaa tcc
//
LOCUS       DASWHZ010000010         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3360260, whole genome shotgun sequence.
ACCESSION   DASWHZ010000010 DASWHZ010000000
VERSION     DASWHZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3360260"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VGA49_00220"
     CDS             complement(<1..452)
                     /locus_tag="VGA49_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664232.1"
                     /translation="MFYADILKIFILTTVSFMAAIAWTPLLTHFLYKYRLGKRIRSSD
                     QAPVMSDLHQHKSGTPVMGGLLIWVTTLVLALVFFYLSVIFPETFFSRLNFLTRSQTL
                     LPLGALVASALVGLVDDYLNIKKVGPNGGGLSILHRLTVYTLVAAVGA"
     gene            complement(532..795)
                     /locus_tag="VGA49_00225"
     CDS             complement(532..795)
                     /locus_tag="VGA49_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664233.1"
                     /translation="MIFLKISPTSEPITLHYNIYFGIDLIGAWYKVYFLPLFGLIVIV
                     VNFVLAYFLHKQEKILSYFLVAAASLAQLILLVAGVIVVLLNI"
     gene            complement(926..>1522)
                     /locus_tag="VGA49_00230"
     CDS             complement(926..>1522)
                     /locus_tag="VGA49_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013680960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9664234.1"
                     /translation="KSMSNIKQERTFIMIKPDGVKRGLTGEILRRIEQVNLKLIALGM
                     FQATEEQIDNHYPKDEKWINRLGEKTMKTYQKYGFDAKSELGTDDTLEIGQMVRGWLI
                     TYMTSAPVVKMVVEGIHAVDMVRKLAGDTIPAFADCGTIRGDFSVDSAASANRDKRAI
                     HNLVHASETPEEAGHEIGYWFAPEDINSYKRVEDDLML"
BASE COUNT          500 a          352 c          280 g          390 t
ORIGIN      
        1 caggcgccaa cagcggccac caaagtatag actgtcagcc ggtgaagaat gctcaagccg
       61 ccgccattgg gtccgacttt tttaatattt aaataatcgt caaccaggcc gaccaatgcc
      121 gaggccacca aagcccctaa aggcaataaa gtctgcgatc tggttaaaaa attaagccgg
      181 ctgaaaaaag tttccgggaa aataaccgat aaataaaaaa agactaaagc caaaactaaa
      241 gtcgtcaccc agatcaacag gccgcccatg accggcgtcc ctgatttatg ctgatgaaga
      301 tcagacataa ccggcgcctg atcagaactt ctgattcttt tgccaagacg atatttatac
      361 aaaaaatgag tcagcaaagg cgtccaggcg atagccgcca tgaatgaaac ggtcgttaag
      421 atgaaaatct taaggatatc agcgtaaaac atatatcaga tttaagttat cagatttaag
      481 ttaccagttt aaattaactt aaatcttaaa tcttacaact ttaatctaga gttaaatatt
      541 aagcaggaca acaattactc cggcgaccag taatattaac tgggctaaac tggccgcggc
      601 caccagaaaa taacttaaaa ttttttcctg tttatggaga aaataagcca gaacaaaatt
      661 gacaactatc acaatcagtc caaacagcgg caaaaaataa actttatacc aggcgccaat
      721 taaatcaatc ccaaagtaaa tattataatg aagcgtgatc ggctccgatg tcggactaat
      781 tttcaaaaaa atcaaaaaag tcaaaatcaa attaatcgcg actgcaacaa aaaaatttac
      841 caaaataaac ttattacgga aaaatgaaga tctgactgaa aataacatat cactataccc
      901 tattaatcta tcccctatac cctaattaaa gcattaaatc atcttccact ctcttataag
      961 aattaatgtc ttccggcgcg aaccagtagc cgatttcatg tccggcttcc tccggcgttt
     1021 ctgaagcgtg gaccaaatta tgaatcgccc gcttatcacg gttggccgaa gcggccgagt
     1081 caaccgaaaa atctcccctg atcgtgccgc agtcggcaaa agcgggaatg gtatcgccgg
     1141 ccagttttct gaccatgtca acggcgtgga tgccctccac gaccatttta accaccgggg
     1201 ctgaagtcat ataagtaatc agccagccac gaaccatttg accgatctcc agggtgtcat
     1261 cagttcccag ttctgacttg gcgtcaaaac cgtatttttg ataggttttc atggtttttt
     1321 cacccagtcg gttaatccat ttttcatctt tgggataatg attgtcaatt tgctcttcag
     1381 tcgcctgaaa cattcccaaa gcaatcaatt tgagattaac ctgctcaatc cggcgcagga
     1441 tttcaccggt caaaccgcgc ttgacgccgt caggtttgat cataataaat gttctttctt
     1501 gtttgatgtt tgacatagat tt
//
LOCUS       DASWHZ010000011         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_784056, whole genome shotgun sequence.
ACCESSION   DASWHZ010000011 DASWHZ010000000
VERSION     DASWHZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784056"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VGA49_00235"
     CDS             <1..1064
                     /locus_tag="VGA49_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX9664235.1"
                     /translation="ILVKFADQLHNLQTLEYLPKEKQDRFVKETLEIYAPIANRLRLG
                     KIKGRLEDWAFKYANPQEFRWLNNLSMKLYAQREQALQHIKEDVRKMLEDENIEVISI
                     HDRAKYLYSLYKKMLKYDLDITRIHDLVALRIIVPTIADCYSVLGLIHRKYRPLKGRI
                     KDYIAQPKPNGYQSLHTTVFAEDGEVVEFQIRTEDMHLEAEYGLAAHWHFKEKGSFKV
                     RKKLAWVNELGKMARQMEDERSLETLKIDIFQNRIFVFTPEGDVIELPEDATPVDFAY
                     QIHTDIGNKCSRTKINDNPAGLDTKLKNGDVVEIIIDKKRKGPSLDWIKFVKTGNARS
                     KIKHFNKKKLTEWEKTLSK"
     gene            complement(1081..1965)
                     /locus_tag="VGA49_00240"
     CDS             complement(1081..1965)
                     /locus_tag="VGA49_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9664236.1"
                     /translation="MNKNKGLGRGLGSLIPPKIQKTVIEETKSEALKEVISGDNVIQI
                     PIEDISPNPSQPRKDFSHRELEDLINSIKEHGIIQPLIVTKKADGDYELIAGERRLRA
                     ARMLELKTVPAIVREAGAQEKLELALIENIQRKDLNPIEEALAYQQLIDQFNLTQEEL
                     SRKVGKSRAFVANTLRLLNLPEEAQKAVIDGKITPGHARTIASLEDPKEQIVFLKKIM
                     ASQMTVREAESAARKISVKKHTRTVTVDPLIVDREEKLREFFGTKVKIKKSGQGGRIE
                     IEFYSDEELNEIVSKLSH"
     gene            complement(1970..2737)
                     /locus_tag="VGA49_00245"
     CDS             complement(1970..2737)
                     /locus_tag="VGA49_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9664237.1"
                     /translation="MAEIIAVVNQKGGVGKTTTAVNLGAALANFGKFILLVDIDPQAN
                     ATSGLGVDYQNLNLGVYEAMIGHQPLKNVILKTAVPGYKIAPTTTALAGANIELINLE
                     DREYKLHQTLLEVRNDYDYILIDCPPSLGILTINGLVAADKVLIPVQSEYYALEGLGQ
                     LLRTVRLVQENLKPDLEIMGAVITMFDKRNSLSQQVLEELYKHFPYQIYRSIIPRNVR
                     LTEAPSHGLSIFNYDPKSKGAKAYERLAREVLNIGFL"
     gene            2904..2986
                     /locus_tag="VGA49_00250"
     tRNA            2904..2986
                     /locus_tag="VGA49_00250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2938..2940,aa:Leu,seq:caa)
     gene            3174..3443
                     /locus_tag="VGA49_00255"
     CDS             3174..3443
                     /locus_tag="VGA49_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9664238.1"
                     /translation="MYYIYILLLSNKQLYKGFTVNLKQRLDEHCRGKVKSTRKRLPVK
                     LIHCECYLLESDAKRREKYLKKTEGRRFLKQQLRDILNKLDINKL"
     gene            3442..3514
                     /locus_tag="VGA49_00260"
     tRNA            3442..3514
                     /locus_tag="VGA49_00260"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3475..3477,aa:Met,seq:cat)
     gene            3693..3765
                     /locus_tag="VGA49_00265"
     tRNA            3693..3765
                     /locus_tag="VGA49_00265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3726..3728,aa:Met,seq:cat)
     gene            3837..>4271
                     /locus_tag="VGA49_00270"
     CDS             3837..>4271
                     /locus_tag="VGA49_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664239.1"
                     /translation="MFLTVHAAVGAVIGQQVGSSLLAFLAGFISHFILDFIPHGDNHM
                     IVDYRNGHKVKRIIFIILADLIGATVLTLGILYLNLSSPSLPFIFWGVIGAIIPDFLV
                     GLYQLYEFRILKKFYAVHTAIHTTLEKYDIKFIHGITIQAIFL"
BASE COUNT         1274 a          975 c          824 g         1198 t
ORIGIN      
        1 tcattctggt taaattcgct gatcagctcc acaatctcca gactctggaa tatctcccca
       61 aagaaaaaca agaccgcttc gtcaaagaaa ccctggaaat ctacgcccca atcgctaatc
      121 gcttaaggct tggaaaaatc aaaggccggc tggaggactg ggcctttaaa tacgccaacc
      181 cccaggaatt cagatggctc aataatttat caatgaaact ttacgcccag cgagaacaag
      241 ctttacaaca tatcaaagaa gacgtcagaa aaatgctgga agatgaaaac attgaagtga
      301 tttccattca tgaccgggct aaatatctct acagtcttta caaaaaaatg ctcaaatacg
      361 atctggacat taccaggatc catgatttgg tcgctttaag aattatcgtc ccgacaatcg
      421 ccgattgtta cagcgttctg ggcctgattc accgcaaata ccgtccgctg aaaggccgaa
      481 tcaaggacta tatcgcccag cccaagccca acggctacca gtcgcttcac acgacggtct
      541 tcgctgaaga cggagaagtg gtggaattcc agatcagaac cgaagacatg cacctggaag
      601 ctgaatacgg tctggcggcc cactggcatt tcaaggaaaa aggctctttt aaagtcagga
      661 aaaaactagc ctgggtcaat gaactgggta aaatggcccg acaaatggaa gatgaaagaa
      721 gtctggaaac tttaaaaatt gatatttttc aaaacagaat ttttgtcttc acgcctgaag
      781 gcgacgtgat tgaactgccc gaggacgcca ctccggttga ttttgcctat cagatccaca
      841 ctgacatcgg caataaatgc tcccggacca aaatcaatga taatccggca gggctggaca
      901 ccaaattaaa aaacggcgat gtggttgaaa taattattga taaaaaaaga aaaggaccca
      961 gcctggactg gatcaaattc gtcaaaaccg gaaacgcccg gtcaaaaatt aaacatttca
     1021 ataaaaagaa actgactgaa tgggaaaaaa ctttaagtaa ataacaatat aaagcgctat
     1081 tcagtggctt aatttactaa ctatctcatt caattcttca tctgaataaa actcaatctc
     1141 aatccggcct ccctggcctg atttttttat tttaacttta gttccaaaaa attctcttaa
     1201 tttttcttct ctatcgacaa ttaaaggatc aacagtcact gtcctggtgt gttttttaac
     1261 agaaatcttc ctggcggcgc tttcagcttc ccgaacagtc atctggctgg ccataatctt
     1321 ttttagaaaa acaatttgct ctttgggatc ttccaggctg gcgattgtcc gggcgtgacc
     1381 gggcgtaatc ttgccgtcaa taaccgcctt ttgggcttct tctggtaaat ttaaaagcct
     1441 taaagtatta gccacgaaag cccggctctt gccaactttt ctggacagct cttcctgggt
     1501 cagattaaac tggtcaatca gctgctgata agccagggct tcttcaatcg gattgaggtc
     1561 ttttctttga atattctcaa tcaaagccag ctccagtttt tcctgagctc cggcttccct
     1621 gacaattgcc ggtacagttt ttagctcaag catcctggcc gcccgcagtc ttctttctcc
     1681 ggcaatcagc tcgtaatcgc catctgcttt tttggtaaca atcaaaggct gaataatccc
     1741 atgttcttta atggaattaa ttaaatcttc cagttcacgg tgggaaaaat cttttctggg
     1801 ctggctggga ttaggcgaaa tgtcttcaat cggaatttga ataacattat caccgctaat
     1861 aacttctttc aaagcctctg atttagtttc ctcaataaca gttttttgta ttttaggcgg
     1921 gatcaaagag cctaatcccc ggcccaaacc tttgtttttg ttcataaatt tataaaaaac
     1981 cgatatttaa aacttcgcgg gccaatcttt cgtatgcttt ggcccctttt gattttggat
     2041 cataattaaa aattgacagg ccatgactcg gcgcttcagt caaccggaca tttcttggaa
     2101 tgattgaccg gtatatctga taaggaaaat gtttgtaaag ttcctccagc acctgctggg
     2161 acaaactgtt gcgtttgtca aacattgtaa tgaccgctcc cataatttcc agatcaggtt
     2221 ttaaattttc ctgaaccaga cggactgtcc gcaatagctg gcccagacct tccaaagcat
     2281 aatattcaga ctggaccggt attaaaactt tatcagcagc caccaaacca ttaatagtca
     2341 ggattcccaa agacggcggg cagtcaatta aaatataatc ataatcattc ctgacttcca
     2401 gcaaagtctg atgaagtttg tattccctgt cttccaggtt aatcagctct atattagctc
     2461 cggccagggc agtggtggtt ggagcaattt tatagccagg gactgctgtt tttaaaatga
     2521 catttttcag aggctggtgg ccaatcatcg cttcatacac acccaggttt aaattttgat
     2581 aatcaacgcc cagacccgaa gtggcgttag cctgaggatc tatgtcaacc agtaaaataa
     2641 attttccaaa attagccaaa gccgctccca aattaacagc tgtcgtggtt ttaccaacgc
     2701 cgcctttttg attaacaacc gctataattt ccgccataga gaagtaaacc ccgttagatc
     2761 cacgcaactt gctccgttag atatgctatc taaaacagat tttaggatct aacggggtaa
     2821 ataatttctt atagtataca ataaccgaac tactttgaca atttggcaat tttattataa
     2881 aattagtttt aaagttataa ttggccgatg tggcggaact ggtatacgcg cgcgactcaa
     2941 aatcgcgtgg tcttcgacca tgtgggttcg actcccacca tcggcaattg acaaacctct
     3001 ttaaaacata tataatatta atactgctta tagcggggta gagaagatgg catctcgcga
     3061 ggcccataac ctcgagatcg cgggttcgcc cgatcatccg atcgggcggc cggtcgaatg
     3121 accggccgag tcccttttaa caattaatct taattcatgc cagtttataa gatatgtact
     3181 atatctatat cttgttactg agcaataagc aattatataa aggttttaca gtcaatctta
     3241 aacaaagact ggatgaacat tgccgcggta aagtaaaatc aacacgcaaa agattaccag
     3301 taaagttaat tcattgtgaa tgctaccttt tagaaagcga cgcaaaaaga agagaaaaat
     3361 atttaaaaaa aaccgaagga agaagatttt taaaacagca gcttagggat atattaaata
     3421 agcttgatat aaataaatta tagcggggta gagaagatgg catctcgcga ggcccataac
     3481 ctcgagatcg cgggttcgag tcccgccccc gcaatttaaa atatccggaa tatcaacctc
     3541 taaatagcaa cgatgatgat agggttcgcc cgatcatcag gtcattcgac ctgacaggtc
     3601 cgatcgggcg gccggtcgaa tgaccggccg agtcccgccc ccgcaatttt tttttacaaa
     3661 acaccaacta ccagtcacca actaccaaat caggcggtgt agctcagtgg ttagagcaat
     3721 gccttcataa ggcagaggtc ggtggttcaa atccacccac cgctataatt ttccacaacc
     3781 tcttttcctc aaaaatgata taatttaata taagtcctta aatatagtta aaacttatgt
     3841 ttttaacagt tcacgcggct gttggcgcgg tcatcggcca gcaggtcggt tcatcattac
     3901 tggcattttt agctggtttt atttcccatt ttattctgga ttttatccca catggcgaca
     3961 atcacatgat tgttgattac cgcaacggtc ataaggtcaa aagaattatt ttcattatcc
     4021 tggctgactt aatcggcgct actgttttaa ctttaggcat cctctatctg aatttatcca
     4081 gcccaagcct gccttttatt ttctggggcg ttatcggggc gataatcccc gacttcctgg
     4141 tcggccttta ccagctctat gaattcagaa ttttaaaaaa attctacgct gttcataccg
     4201 ctatccacac tacccttgaa aaatatgaca tcaaatttat ccatggcatt accattcagg
     4261 caatctttct c
//
LOCUS       DASWHZ010000012         5995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2627592, whole genome shotgun sequence.
ACCESSION   DASWHZ010000012 DASWHZ010000000
VERSION     DASWHZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627592"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGA49_00275"
     CDS             complement(<1..327)
                     /locus_tag="VGA49_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664240.1"
                     /translation="MSFRQFFQRLLMAIVVVGFTANWAEAQEDNQGILIYEDDDDQSD
                     QPANALAVSSDDPAEAADVKVVLDYVAIAAYFIKRPYFSEEYVWAASFERRLFCDYIS
                     IVGLGAI"
     gene            473..2527
                     /gene="gyrB"
                     /locus_tag="VGA49_00280"
                     /pseudo
     CDS             473..2527
                     /gene="gyrB"
                     /locus_tag="VGA49_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008277801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
     gene            2679..3641
                     /locus_tag="VGA49_00285"
     CDS             2679..3641
                     /locus_tag="VGA49_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664241.1"
                     /translation="MIIGGVKFHPAINASGARGFDGTGYWFHSILKPFGLNYDESTFV
                     AKTTTLKPRQGNMRSTTWFPDCIMVKLLKGITLNAVGLTGPGAEALFELAGWQERTKP
                     FFLSFMSVASNQDGRLAELENFVQLLAKELSAFQTRIGLKINFSCPNVGLNPSKLIGE
                     VSQAMDICRQLNIPIVPKFNALLPFEIGVEISKDPACSGLCVSNTIPWNSTNRINWQR
                     LFGVNDSPLAKYGGGGLGGAPLLPLVLDWIRSARRHGLTKPIIGGGGILSKKDAKAMV
                     LTGASGISLGSISLLRPWRIAGIIRYVNKLYRRLENENKDCSAL"
     gene            3613..4614
                     /gene="pyrC"
                     /locus_tag="VGA49_00290"
     CDS             3613..4614
                     /gene="pyrC"
                     /locus_tag="VGA49_00290"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9664242.1"
                     /translation="MKIKIAPPFDTHVHFRQKAMLTVVAPLSAVHFGRVIVEPNTTPV
                     IGPVNVEAYKKRIESHAPGVDVLMTIYLQPTTTPADIQKAHELGIRIAKLYPEGVTTG
                     SESGVSNISDLFDVFAEMEKLGWVLQLHGETPGIFCLDREANFAEHTLKMLVNHFPRL
                     RLTMEHLSSKAAGEIVVSLPDTVVASVTVHHLLITLDDVIGDKINPHLFCKPVAKRSE
                     DRDWLIATVMSGHSKLFLGTDSAPHLRERKECAEGCAGIFTGPVAMPLLAGLFDKYGE
                     LDQLEDFTSGRANRFYRQKKVEGQIDLVEIPWIVPQDYNGVVPFRAGQELNWQVRRI"
     gene            complement(4696..5013)
                     /locus_tag="VGA49_00295"
     CDS             complement(4696..5013)
                     /locus_tag="VGA49_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664243.1"
                     /translation="MTYFSRDYILFFRRQPYLRRCSVKNRIKNFFDPVSEKKKQAWAD
                     VIRRERVVVLWGERDEMIFVFPNNTGFVIDAAGQTKPLPLKDVRDFLNLLELISKKKE
                     EEQ"
     gene            5220..5435
                     /gene="secE"
                     /locus_tag="VGA49_00300"
     CDS             5220..5435
                     /gene="secE"
                     /locus_tag="VGA49_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX9664244.1"
                     /translation="MPKLKSLVLDNRLSRYIIESKRELKKVVWPTRKETMNHTFIVIV
                     ISLGVAIFLGALDFVFSFGIERLLLLR"
     gene            5450..5995
                     /gene="nusG"
                     /locus_tag="VGA49_00305"
     CDS             5450..5995
                     /gene="nusG"
                     /locus_tag="VGA49_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX9664245.1"
                     /translation="MPKQTTLYGRRWYVIHTYSGYEENVKRNMEQRIESMDMEDKIFQ
                     VLIPTEKKIKIKGGKRKIVTEKLFPGYVLVQMIVTDDSWYVVRNTPNVTGFVGTGTTP
                     TPISDEEIKGLQKRMGVEEPTYKIDVTVGSPVKIVDGPFKNFEGKIAEIDESRGKVKV
                     LVSMFGRETPVELDFLQVQKI"
BASE COUNT         1707 a         1453 c         1356 g         1479 t
ORIGIN      
        1 gatcgccccg agtccaacga ttgaaatgta gtcacagaac agccgccgtt caaatgacgc
       61 cgcccaaaca tactcttcgc tgaaatacgg ccgcttgatg aaataggcgg cgatagcaac
      121 atagtccaga acgactttaa cgtcagctgc ttcagccggg tcgtcgctgc taacagccag
      181 ggcattggcc ggctgatccg actgatcgtc atcgtcctcg taaatcagga taccctgatt
      241 gtcctcctgg gcctctgccc agttggccgt aaagccgacg accacaatgg ccatcaacaa
      301 ccgctggaaa aattgacgga aagacatttt atctcctccc tccgtagaat gtgcgttcct
      361 attcaaactt ccctgcacct tatctaaaag caggttattt gtcaaggttc aatttagcct
      421 tgcctaaaag gctttttttt gataaaataa ttaaatagtt taatcaatca atatggctaa
      481 aattaaaaaa agcacagcaa aacccggcag tggcctggcg gccaaaagcc aatacacggc
      541 caaagaaatt acagttttaa aaggtcttga cccggtcagg aaaagaccgg ggatgtatat
      601 tggcacgacc ggcgaggccg gcctccatca tttaatctgg gaagtggttg acaacgccat
      661 tgacgaggcc attgccggct attgtactga catcactgtc agattattac ccgacaacaa
      721 ggttgaaatt tctgacaacg gccggggcat tccggtggac acgcataaag aaactaaaaa
      781 gtcagctttg gaaactgttt taaccaccct tcacgccggc ggttaatttg gcgccggcgg
      841 ctacaaagtt tccggcggtc tgcatggtgt tggtgtttcg gtcgttaacg ctttgtcagc
      901 ctgggtgaaa gctgaagtca ggcgggacgg ccagatttgg gttcaggaat acaagagagg
      961 caaaccagtc gctaaagtta aaccagtcgg caaaaccaaa cagaccggca cgactattac
     1021 ctttgatccc gatcctgatg ttttccctga aataaaatgg aactggaatt taattttaaa
     1081 ccggttacgg caatacgctt atctgaccaa aaaaattaaa atcattattt ttgatcagcg
     1141 ggaaaaaggt cagggaaaag attacacttt ttattttgaa ggcggcattg ccacttatgt
     1201 ccgttacctc aacaccaata aagaaatcaa gcatccaacc attttttata ttgaaaaaga
     1261 actggatgaa attcaggtgg aaattgcttt gcaatacact gatgattatc aggaaacagt
     1321 ttacactttt gccaataaca ttttaaaccc cgggggcggc acccatttag ttggctttaa
     1381 aacagcttta accaggactc tcaacaactt tgcccgggcc aaaaattacc tcaaagaaaa
     1441 agatgacaat ttaaccggtg aagatgtccg ggaaggtctg acggccattg tcagcgtcaa
     1501 aatcaaagaa cctcagtttg aaggccagac taaagacaag ctgggcaacc cggaagtccg
     1561 gacaattgtt gattcggttt tttccgaagc ctttgcttat tttctggaag aaaaccccgg
     1621 caactctgaa aaaattattg aaaaatgtat tatcgcttcc cgggccagag cggccgccaa
     1681 atcagcccgg gaaacaattt taagaaaagg cgtcctggaa ggcctggctt tgcccggcaa
     1741 gctggctgat tgttcctcca aagaacccgg ggaatgcgaa ctctatattg tagagggcga
     1801 ttcagccggc ggctcagcca aacaaggccg ggaccgggaa tttcaggcta tcctgccgat
     1861 gtggggtaaa atgatcaacg ttgaaaaaac ccggctggat aaaattctaa cttctgacaa
     1921 aatcaaacca ttaattatcg ccctgggaac caatatcggt gaacaatttg aactggacaa
     1981 attaagatac gaccggatta ttattatggc tgacgctgat gttgacggct cccatatccg
     2041 gacattgctc ttaactttat tttaccgtta ttttaaacca ttgattgagc aggggcatat
     2101 ttacattgcc cagccgccgc tttacaaaat ccaaaaaggc gctgaagctc actacgctta
     2161 tactgacgaa gagaaagaaa aaatcctggc tgacatggcg gcggcggcca aaaccaaacc
     2221 ggccaaaaaa tcggcccaaa ccaaaacaga cgaaactcca accgaaggag aggcgggcga
     2281 gggcgttgaa gtgattgaag gtttaggtcg ggttaatatt cagcgctata aaggtttggg
     2341 agaaatgaat cccggtcagc tctgggaaac cacgatggat cccaacagcc ggattatgaa
     2401 aaaaattacc attgaagacg cccagcgggc cgaccaaatt tttgaaattt taatgggctc
     2461 ggaagtcgct ccccggaaac gtttcattca gacacgggcg aaagaggtgg agaatctgga
     2521 tatctaattt ggtagttggt ggttggtacc cggcagatgc cgggtttttt tatttttgtg
     2581 aaatccaaaa ctcgaatttc aaaatttttg acatttaaca atcatctggt taccatatac
     2641 taaaccgttc cttaacaacc taactcacag ggggtgtcat gataattggt ggcgtcaagt
     2701 tccatcctgc aattaacgcc tccggcgcca gaggatttga tggaaccggc tactggtttc
     2761 attccatcct caagccgttt ggcctcaact atgacgagtc aacttttgtc gccaaaacaa
     2821 ccacgcttaa accccgccag gggaacatga gatcgactac ctggtttcct gactgtatta
     2881 tggttaaact cttgaagggt ataaccctca atgcagtagg gctaaccggc cctggagctg
     2941 aagccctttt tgaactagcc ggctggcagg agagaactaa accgttcttc ctgtcgttta
     3001 tgtcagtcgc cagcaaccaa gacggccggc tggctgaatt ggaaaacttt gttcagttgt
     3061 tggccaaaga actgtcggcg ttccagaccc gaattggttt gaaaataaac ttttcctgtc
     3121 ccaatgtcgg cctcaacccc tccaaactga ttggcgaagt cagccaggcc atggacattt
     3181 gccgccagct gaacattcca atagtcccca agttcaacgc tctcctgccg tttgaaattg
     3241 gggtggaaat ctccaaagac ccggcctgct ccggactctg cgtttctaac actattccct
     3301 ggaacagcac aaatcgaatc aactggcagc gcctctttgg cgtaaatgac tcgccactgg
     3361 ctaagtatgg cgggggcggt ctgggcggcg ctccgctcct gcccctggtt cttgattgga
     3421 tcagatcagc ccgcagacac ggtttaacca aaccaatcat tggcggcggc ggcatcctgt
     3481 caaaaaaaga cgccaaagca atggttctaa ccggcgccag cggcatttct ctgggatcaa
     3541 tttccctgct ccgaccctgg cggatcgccg ggatcattcg ttatgtcaac aaactctatc
     3601 ggaggctgga aaatgaaaat aaagattgct ccgccctttg acactcatgt tcatttccgg
     3661 caaaaagcca tgctgaccgt ggttgctcct ctgtcagccg tacacttcgg ccgggtgatc
     3721 gttgaaccta ataccacccc ggtcatcgga ccggtcaatg tcgaagccta caaaaaaaga
     3781 attgaaagcc acgctcccgg cgtagatgtc ctgatgacga tctatcttca gccaacaacc
     3841 acgccggctg acatccaaaa agcccatgaa ctcggtatca ggattgccaa gctttatcct
     3901 gaaggagtaa cgaccggttc ggaaagcggc gtcagcaaca tcagcgatct gttcgatgtt
     3961 tttgctgaaa tggaaaagtt aggatgggtc cttcagctcc acggagaaac tccaggcatc
     4021 ttctgtctgg accgggaagc caattttgct gaacataccc tgaaaatgct ggtcaatcat
     4081 tttccccgcc tgcggctgac aatggagcat ctgtcatcca aagcggccgg agaaattgtc
     4141 gtcagcctgc ctgacactgt ggttgcttca gtcacggtcc atcatctgct gatcactttg
     4201 gatgatgtca ttggcgacaa aatcaaccct catctcttct gcaagccagt cgccaaacgg
     4261 tcggaagacc gcgactggct gattgcaact gtcatgagcg gccattccaa gttatttctt
     4321 ggaacagatt cggctcctca tctgcgggaa agaaaagaat gcgctgaagg ctgcgctggg
     4381 atttttaccg gacctgttgc catgccgctg ctggcaggcc tgttcgataa atatggcgaa
     4441 cttgaccaac tggaagattt tacttccggc agagccaaca ggttttaccg tcagaagaag
     4501 gttgaaggtc agattgacct ggttgaaatc ccctggatcg tgcctcaaga ctacaacggc
     4561 gtcgtgccgt tcagggcagg ccaagaactt aactggcagg tcagacgaat ctaaaaacac
     4621 cataagacta tttcggaggg agatagtctt tttttattaa aatgaaaagc tgaccccacg
     4681 aatggcgcca gcttcttatt gttcctcttc ctttttcttg ctgatcagct ccagcaaatt
     4741 cagaaaatcc ctgacatctt tcagcggcaa aggcttagtc tggccagcgg catctatgac
     4801 aaaaccggta ttattgggaa aaacaaaaat catttcatct ctctcacccc acaatacgac
     4861 aactctttcc cgcctgatga catcagccca ggcttgcttt ttcttttcag agaccgggtc
     4921 aaaaaaattt tttatccgat ttttgacgga acacctcctt aggtacggtt gcctcctgaa
     4981 aaacagtata taatcgcggc tgaaataagt caaaattatc cctcttgatt ttttaaaaca
     5041 aatagtctat actataaaaa acattaatca cggcccgcag gctgttttta aatagcctga
     5101 aaacagacac taggcaacag gcacaaggca ccagggttgg aaaatttttt ttctattgcc
     5161 tgctcccttt tgcctcgcct tgtgccttat gcctcgtgcc tattgcctta gttaaaaata
     5221 tgcccaaact aaaaagctta gtattagaca atcgactatc acgctatatt attgaatcaa
     5281 agcgtgaact aaaaaaagtc gtctggccga ccagaaaaga aacaatgaac cacaccttta
     5341 tcgtgattgt catcagcctg ggggtggcta tctttctggg cgccctggat ttcgtctttt
     5401 cttttggaat tgaacgcttg cttttactta ggtaattaag ctaaaatata tgccaaaaca
     5461 aacaacacta tacggccgac gctggtatgt catccatacc tactcgggct atgaagaaaa
     5521 tgtcaaacgg aacatggagc aaaggattga aagcatggac atggaagaca aaattttcca
     5581 ggttttaatt ccgactgaaa agaaaattaa aattaaaggc ggcaaaagaa aaattgtcac
     5641 tgaaaagctt tttcccggct atgttctggt tcagatgatt gtcactgatg attcctggta
     5701 tgtcgtccgg aacactccca atgtcaccgg ctttgtcggc accggcacca cccccactcc
     5761 gatctctgat gaagaaatca aagggctgca aaagagaatg ggcgttgaag agccaaccta
     5821 caagattgac gtcactgtcg gctcaccggt taaaatcgtt gacggaccgt ttaaaaactt
     5881 tgaaggcaaa attgctgaaa ttgacgaatc ccggggtaaa gtcaaagtac tggtttcaat
     5941 gttcggccgg gaaactccgg tggaacttga tttccttcag gtccaaaaaa tctaa
//
LOCUS       DASWHZ010000013         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3008199, whole genome shotgun sequence.
ACCESSION   DASWHZ010000013 DASWHZ010000000
VERSION     DASWHZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008199"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VGA49_00310"
     CDS             complement(<1..713)
                     /locus_tag="VGA49_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9664246.1"
                     /translation="MKKCLSLVLLLLILTGVRCPTQKTTDNVAEVSSVQSETVSFTTS
                     DGLNIAASYFPGPANAQNSVILVHMLAQNRHDWDSLAGKLRQGDFNVLSIDLRGHGQS
                     DGQYQKFDNSDFAKMVFDVAAAVQFLKEKHPETTIKLIGASLGANVALNAAVIDPAVS
                     GLVLLSPGLEYRSVTTEDAIEQYTSALLLVASQDDKASYLAARVLFDLVPESDHYKYL
                     QTYKYAGHGTHMLTAVDDL"
     gene            complement(907..1401)
                     /locus_tag="VGA49_00315"
     CDS             complement(907..1401)
                     /locus_tag="VGA49_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HEX9664247.1"
                     /translation="MKFAIISDTHDNLVTLHQALDWIKKNKIKLIIHCGDVTTRETLL
                     EIVNNFDGSVQVVAGNADQNFWRRDNFSQYKNLKLYKDVGELEIDNLKIAFCHFPDKV
                     KDLARKDFNLVFYGHTHRPWQEKINNTEVINPGNLAGLFYKASFAVFDTTTKKVELKI
                     LEML"
BASE COUNT          477 a          317 c          301 g          439 t
ORIGIN      
        1 tccagatcgt caacggctgt caacatatga gtcccgtgac cggcgtactt ataagtctgc
       61 aaatatttat aatgatctga ttccggaacc agatcaaaca aaacgcgggc ggccagataa
      121 ctagccttat cgtcctggct ggcgactagc agtaaggcgc tggtgtactg ttcaatagca
      181 tcttccgttg ttacgctccg atattccagg ccgggtgaca gtaaaaccag gcctgacact
      241 gccggatcaa ttactgccgc gtttaaagcc acattggctc ccagactggc gccaatcaat
      301 ttaatggtcg tttccggatg tttctctttt aaaaattgaa cggcggccgc gacatcaaaa
      361 accattttgg caaaatcgct gttatcaaat ttctgatatt ggccatcgct ttgaccgtgg
      421 cctcgtaaat caatggacaa tacattaaag tccccttgcc ggagcttccc cgctaaacta
      481 tcccagtcat gacgattttg ggccagcata tgaaccagaa tgactgaatt ttgagcattg
      541 gccggaccgg gaaaataact ggcggcgata ttcaggccgt cgctggtcgt aaaactgact
      601 gtttcgctct gaacagagct aacctccgca acattatcag tggttttctg tgtcggacat
      661 cgcactcccg tcaaaattaa taacaaaaga accaaggata gacatttctt cataatatac
      721 gattctaata tactaattga tactaaagat acttatactc cgattctaat atacgaatgg
      781 atacgaaaga taccaataaa gatattaata tccgtatgaa ttagtatatt agtatcacaa
      841 aattggtttc attagtatcc atacgtatat tggcatcgtt tttaaaatta taacaaatat
      901 tttaaattaa agcatctcca aaattttcaa ctctactttt ttggtcgtgg tgtcaaagac
      961 ggcgaagctg gctttataga acagcccagc cagattgccg gggttaatca cttcggtatt
     1021 attgattttc tcctgccagg gtcggtgagt gtggccgtaa aaaactaagt taaaatcttt
     1081 tcttgccagg tctttaactt tgtcaggaaa atgacaaaaa gcaattttaa gattatctat
     1141 ttctaattcg ccaacatcct tataaagttt taaattttta tattgtgaaa agttatcacg
     1201 ccgccaaaaa ttctgatcag cattgccggc aacgacctga accgaaccgt caaaattatt
     1261 gacgatttcc agtaaagtct ctctggtggt aacatcaccg caatggataa tcagtttgat
     1321 cttgttcttt tttatccagt ccagagcctg gtggagggtg acgaggttgt cgtgggtatc
     1381 ggaaataatc gcaaacttca tacttgaatt agagaattaa gcaacagatt aagtaaaaag
     1441 ttaaaaataa gaagtaaaaa ataaaagtta aaaatcaaaa gttaagttta gataattcat
     1501 cgcgaagcca ggcaatagcc tggcgaagcg atac
//
LOCUS       DASWHZ010000014         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2632090, whole genome shotgun sequence.
ACCESSION   DASWHZ010000014 DASWHZ010000000
VERSION     DASWHZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632090"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            796..1107
                     /locus_tag="VGA49_00320"
     CDS             796..1107
                     /locus_tag="VGA49_00320"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664248.1"
                     /translation="MAVFSLPFVFLAGLSQLRSTTFDSGGFNLEELTRDSFGVEIPEN
                     INFNQGGDFEEFSKDTDGDGLPDFLEEILLTNPNNPDTDGDGVNDGAEYEAGTNPLEA
                     E"
     gene            1215..2021
                     /gene="murI"
                     /locus_tag="VGA49_00325"
     CDS             1215..2021
                     /gene="murI"
                     /locus_tag="VGA49_00325"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9664249.1"
                     /translation="MKIGVFDSGFGGLTIFKEIHQRLPEYDYIYLGDNARSPYGSRSP
                     ELIYKFTIQAVEYLFKHNCRIIIIACNTASAEALRKLQQEYLPKEYPDRRVLGVIRPM
                     AEEVAQVTKGRVGVIGTDGTVKSGAYIKEFEEVDPKIKVFQKASPLLVPLVEEGWHKS
                     EVTKKVLRKYLKPLKDKRVDTLILGCTHYPVLLKDIRAIMGKNCHVPDPGKVVVDKFY
                     DYLNRHPEIAAKLSKKGTVKFLTTNSTEKFNRIGSKLYGEQVRAEKVELD"
     gene            2167..2239
                     /locus_tag="VGA49_00330"
     tRNA            2167..2239
                     /locus_tag="VGA49_00330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2201..2203,aa:Ser,seq:tga)
     gene            complement(2496..2642)
                     /locus_tag="VGA49_00335"
                     /pseudo
     CDS             complement(2496..2642)
                     /locus_tag="VGA49_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
     gene            complement(2686..2988)
                     /locus_tag="VGA49_00340"
     CDS             complement(2686..2988)
                     /locus_tag="VGA49_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9664250.1"
                     /translation="MNRAGTQDITTKEKNSIIRILKKHNVKKASLFGSMARGEATSKS
                     DVDFLIEFKTKKSLLDLASLRLELEETLNTDVDVLTYKSLHPLLKKKILREQKVIL"
     gene            complement(3084..3581)
                     /locus_tag="VGA49_00345"
     CDS             complement(3084..3581)
                     /locus_tag="VGA49_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664251.1"
                     /translation="MINLFLIILMIFNFLTLVRTSDIFNIIEKNKPAIPRTEQTIIPG
                     PSPKTQISRPVIIYPADGSTVEGVIIKGAAKPNSLIWAYVNYPEDELKYLSINSYANG
                     GASRVDQNGNFEFALAEACARELTVVVAIVDEPLTTGPFKAKNVSKPITYTNTGKFSE
                     ICYRE"
     gene            complement(3712..3999)
                     /locus_tag="VGA49_00350"
     CDS             complement(3712..3999)
                     /locus_tag="VGA49_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9664252.1"
                     /translation="MENKKIPIKSFKDLIVYQNTYKAMLIVFRGILPKLPDSEKFDLK
                     NQLSRSTKAIPRLIAEGFAKKHQKAGFQKYMDDAMAESNETIVSLAIENEQ"
     gene            complement(4279..>4821)
                     /locus_tag="VGA49_00355"
     CDS             complement(4279..>4821)
                     /locus_tag="VGA49_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9664253.1"
                     /translation="TQHLEFARMIAKRFNTIFGETFVVPEQYTVELSSRIMGLDDPLK
                     KMSKSADNPNNYIALTDSPDVIRKKFKKAVTDSGGEIKYSQSKLAIMNLMDIYQSLTG
                     DSIKAIEKKYQGKGYADFKNDLAEIVIDYLKPLQSKLEDLNKDPDYVKEILKEGADQA
                     RKLASKKLTEVKEKMGYVEF"
BASE COUNT         1330 a          916 c         1018 g         1557 t
ORIGIN      
        1 ttagcaactc ataattaata accattaatc aaaaaaccta tgcccgaaga aacaaaatca
       61 gttaacctaa ctaaggttag caatctcctg tcccaaacct ggaactattt cgtcgccaac
      121 tggaaaaaat tcgttttgat atttttaatc cagatagctg tttatttggc gctgggagcc
      181 ttgttcggct ttttgggagt ggccgggttt agtcgcctgg gcggctttgg agcggcgatc
      241 gcgatgtttt tgctctatgt cctgatattc gtctggagca tctggatgtc ggtcgccatg
      301 attttcgtgg ttaaagacaa tcaattgaca gtaggtcagg ccatcggcca ggccaaagcc
      361 agactgggca gcgccatttt gatcggaatt ttactggcgg tgattgtcac cgtcggttat
      421 ctattgttga ttgttcccgg aattattttt actgtctggt acggcctggt tttatatgtt
      481 ttggtttttg aaggcaagag cggctggtcg gcttttcaaa ggagccggga actggtcaga
      541 ggctatggtt ggtcaatttt catcaggctg atcgtgatca tgctgattca gctgatagtc
      601 agcctggtca gcgccattcc ggttattggc tggctgatag cgattgtttc gccgtttatt
      661 ttgaccggct actggatgat ttatctggga cagatttatc aggatttagt aaaaattaaa
      721 tctcaagacg cccagtccaa agaaactgtc tccgttggcg ctaaaatcgg tttggtgatt
      781 ttagttttgg cgttaatggc cgtcttttca ttgccatttg tatttttagc cggccttagt
      841 cagttgcggt caacgacatt tgattcaggc ggttttaatc tggaagaact gaccagggac
      901 agttttggag tggaaattcc cgaaaacata aattttaacc agggcggtga ttttgaggaa
      961 ttttcaaaag acactgacgg cgacggcctg cctgattttc tggaagaaat tcttttaacc
     1021 aatcccaaca atccggatac ggacggcgac ggggtgaatg atggggctga atatgaggct
     1081 gggactaatc cgctggaggc ggagtaaggg ttaaaaaaat tctaaatcca aaattcgaaa
     1141 tcctaaataa gcactaaata caaaattata aattcaaaac ctgttaattt ttgtaaagat
     1201 aattacattt agttatgaaa attggtgtat ttgactctgg tttcggtggc ttaactatat
     1261 tcaaagaaat tcaccagcgc ctgccagaat acgactatat ttatctgggt gacaacgccc
     1321 gcagtcccta cggcagccgg tcaccggagt tgatttataa atttaccatt caggcggttg
     1381 aatatttgtt taaacacaat tgccggatta ttataatcgc ctgcaatacg gcttcggccg
     1441 aagctttgag aaaactccag caggaatatc tgccaaaaga gtatcctgac cggcgggtgt
     1501 tgggagtgat caggccgatg gccgaagaag tggctcaagt taccaaaggc cgggtggggg
     1561 tgattgggac tgacgggaca gttaagagcg gagcttatat taaagaattt gaagaagttg
     1621 atccaaaaat caaagttttt caaaaagcca gccctttatt agttcctttg gttgaagaag
     1681 gctggcataa aagcgaggtt accaaaaaag ttttgagaaa atatttaaaa ccgctcaaag
     1741 ataaacgggt tgatactttg attttaggct gtactcatta tccagttttg ctgaaagata
     1801 ttcgggcgat catgggtaag aactgccatg tcccggatcc gggcaaagtc gtggtggata
     1861 aattttatga ttatttgaac cggcatccgg agattgccgc taaattatca aaaaagggga
     1921 ctgttaaatt tttaacgact aattcaacgg aaaaatttaa ccggattggg agcaagttgt
     1981 atggggagca ggtcagggcg gagaaagttg aattagatta gctcgcgtcc ttgacaaggg
     2041 ttttcaatat gctataatag atttaggtag tgaaccatta gattactggt ttaactctac
     2101 ttgtcacata agaggcctct tccccggaag aggtttttat gttattattt tcacgtggtg
     2161 gaccacgggc cagtagtgtt aatggttagc acactacctt tgacaagtag aggagcgggt
     2221 tcgatccccg tctggtccac ccgagaaatt atggtgtcgg ctaacgccga ctttttaaat
     2281 tcaaatttct gttcttttgg aaccaaaaga accaaaagtt ctggggtagc cgattcgcct
     2341 gtgtgctatt agggtaatca gcgtctgccc tgggctcaag gctaccccag accccttggc
     2401 gcattcagca atttttgtag ttttaatttt ttttaaacta accaaaaaag actagttcgc
     2461 ggagatgact agtctttttt agtttataga tcaatttata aattttcttg tattttaaca
     2521 agttttttct taagatttgg aatatctttg actactacct gcccggttag gtctaaatca
     2581 attccaaaat attcatggat cagtatatcc ctcatgcctg ctatttcttt ccaggaaatt
     2641 tccgggtatt tttgctgtgt tcaacatata tgccagcttc ttttttcata ggatcacttt
     2701 ttgttcccga agtatttttt tcttaagcag aggatgaaga gacttgtaag tcaggacatc
     2761 gacatctgta ttgagcgttt cttcaagctc cagccttaag ctggccagat cgagcaggct
     2821 ttttttcgtt ttaaattcta tcaaaaaatc aacatcactt ttggaggtag cttctcctct
     2881 ggccatagag ccaaaaagag aggctttttt gacattatgt tttttaagaa ttctgataat
     2941 agaatttttt tcttttgttg ttatgtcttg agttccggct ctgttcatat ttgtttatta
     3001 atattatatc ttaaatttaa cacaaataga gtcttttttc aatttttaaa ttatcaaaaa
     3061 agactagttc gtggattatg aatttattcc ctataacaaa tctccgagaa tttaccggta
     3121 ttagtgtatg taatcggctt actgacattt ttcgctttaa aagggccggt tgttaatggt
     3181 tcatcaacaa tagcaacaac tacggtcagt tcccgggcgc aggcttcagc cagggcaaat
     3241 tcaaaattgc cgttttgatc cactcgcgaa gcgccgccat tggcgtagga atttattgaa
     3301 agatatttta gttcgtcttc cggatagtta acataggccc agatcaaact gttgggtttg
     3361 gccgcgcctt ttattataac gccttcaacc gttgagccgt cggccggata gatgatgact
     3421 ggtctggaga tttgtgtttt tggagacggg ccgggaataa tcgtttgttc cgtccggggg
     3481 attgccggtt tgtttttttc aataatatta aaaatgtcgc tcgtccggac cagcgttaga
     3541 aaattgaaaa tcatcagaat gattagaaat agattgatca taatttttaa attttttgat
     3601 ccagccccgg attattataa ttttataaca attcagggct gaagggaaag ttcgtgagag
     3661 agtggtgaac tttccctggc ttggaaatga caaacccctt ctgttgtgtt tttactgctc
     3721 attctcaatt gctaaactga ctatagtttc attagattca gccatagcgt catccatata
     3781 tttctgaaaa ccggcttttt ggtgtttttt ggcaaatcct tcagcaatta atcggggaat
     3841 agctttggta gatctgctta actgattttt taaatcaaat ttttcactat ctggaagttt
     3901 tggaagaatt cccctaaaaa ctatcagcat agctttatat gtattttgat agacaatcaa
     3961 atctttgaat gatttgatag gaattttctt gttttccata ttcttattat tactgttaac
     4021 tattaactaa agacaatacc tgccgccaaa accagaccca aaccctaacc agaaattttt
     4081 aataataaaa accacccgct ctacaaaagg gggtggaaat cttttaatat tataacttgt
     4141 taaattctaa aagtaaacaa aattcccata ttcttattat taccattaac taataactaa
     4201 agacaatacc cgccgccatg accaaaaacc aaaaaccacc ccagggtggt atcttcaatc
     4261 atcatacatt agccaatcct aaaattccac gtatcccatc ttctccttaa cttcagtcag
     4321 ctttttactg gctagttttc tggcctgatc agcgccttct ttaagaattt ctttaacata
     4381 atcaggatct ttgtttaaat cttctagttt agactgaagc ggctttaaat aatcaatgac
     4441 aatttcggcc agatcgtttt taaaatcagc gtaaccttta ccctgatatt ttttttcaat
     4501 cgctttgata gaatcgccgg ttagcgactg ataaatatcc atcagattca taatggccag
     4561 cttgctttgg gaatatttta tttcaccgcc ggaatctgtc accgcttttt taaatttttt
     4621 gcggataaca tccggtgaat cagtcagagc gatgtaattg ttgggattgt cagcgctttt
     4681 ggacattttt ttaagcggat catccagacc cattatccgg gagcttaact ccacggtgta
     4741 ttgttcgggc acgacgaacg tttctccaaa aattgtgtta aatcgtttgg cgatcatccg
     4801 ggcaaattcc agatgctggg t
//
LOCUS       DASWHZ010000015         3983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_46252, whole genome shotgun sequence.
ACCESSION   DASWHZ010000015 DASWHZ010000000
VERSION     DASWHZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46252"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGA49_00360"
     CDS             complement(<1..941)
                     /locus_tag="VGA49_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX9664254.1"
                     /translation="MKKIVFIGGGGGSKNILPGLKNDFDCVAVVTTFDNGGSYGQLRY
                     QYQTVLSGDVRDALIALADNANLADIWQWRFAAGDFKGHSLGNLFLANLYDNLKDYNQ
                     VLKLASEILKVKGKVGPVSFDWAELTAELTDGTIIAGETKIDEVEPGVKRSPIKKVYL
                     SRPRRQSRRDKPVKVNPEVVEAIVEADLIIIGPGDLYTSLIPNFLPTGVTEAIKKSKA
                     KKVYFINKFTTLGQTDSLTAFDHAVEIENYLGSSLDVVVINSSLVSDKILEHYRKRNE
                     RLVEVDLERLKSGPYQIIEADLLADEFVEKAKSDKLK"
     gene            complement(1079..2848)
                     /gene="glmS"
                     /locus_tag="VGA49_00365"
     CDS             complement(1079..2848)
                     /gene="glmS"
                     /locus_tag="VGA49_00365"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9664255.1"
                     /translation="MCGIISYKGQQPAPKIILAGLKKLEYRGYDSWGIAVKDQEINLL
                     KKIGKVGEIEAKDLDFKSQGDQIAIGHSRWATTGKVTEANAHPHLSNNKKIALVQNGI
                     VENYLTLKKELQAKGYIFNSETDTEVIVNLIQEYNRKNDFVQAVRKAFNRLTGRNAIV
                     AIHQDNNLIVGARLGSPLIVGIGDNEYFLASDIPAFLNYTRRVNYLDDGELVVIDQSP
                     RFFSLKTGQEILKRLIEIDWQADQAEKGDYRHFMIKEIMEQKETIRRAVEQDEKAILK
                     IARDIKNAFGTFLVGAGTAGKVCFTGDYFFSKIAGEHTNFVPASEFINYSHFLTPKSL
                     MVVVSQSGETADVLEAMEIAKAKGVKIISLVNVQGSSIMRQSDYSLLIKAGPEQAVAS
                     TKATTAQMSILALLAYAINDQLARGKTNLVALAGKVNDMLNPRYEDHIRQLAEKIHKH
                     ESMYIIGRGVNYPMALECAIKLQEVSYIHAEGFAGGELKHGPIALIGEGTPLITLVAN
                     DENRESILSNAQEVKSRGGYIIGVSPFPSDVFDYWIKVPEVGPLSPIVNIIPVQILAY
                     HLGVLKGNDPDKPRNLAKSVTVK"
     gene            complement(2880..3500)
                     /locus_tag="VGA49_00370"
     CDS             complement(2880..3500)
                     /locus_tag="VGA49_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664256.1"
                     /translation="MTIFVDFDHTLFNATLFKEKLYDVLSYTGFGKEEFFEIYNQVQS
                     EDGWNDYKLIKALKSKKDFDENKALISLRALIQSTSDYLYLDAVSFLNLFQNKARLIL
                     VSWGDPNIQKDKVLNSGLMKYFDKTIFTSQKKYSLLADYLDKDSVNFFINDNPLENQE
                     VAKIYPDMKILTIKREDGKEYQANDYENLKTLTDLQTISDVINKNL"
     gene            complement(3578..>3983)
                     /locus_tag="VGA49_00375"
     CDS             complement(3578..>3983)
                     /locus_tag="VGA49_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664257.1"
                     /translation="NRVFREKKPTLALITIKLPDYNDWRQTFYDFGRVVRDQAGNIQE
                     IVELKDTTDQQKSITEVNPSYYCFEADWLWKNLDKLSTNNQQGEYYLTDLIKIAFDQG
                     CQIETISCANPLEGLGINTPEQLEIARKATSH"
BASE COUNT         1129 a          923 c          729 g         1202 t
ORIGIN      
        1 ctttttaatt tatcactttt tgccttttcc acaaattcat ccgccagcaa atcggcttca
       61 atgatctgat acggcccgct ctttaatcgt tccaaatcaa cttccactaa gcgctcattt
      121 ctttttctat aatgttctaa aattttatct gaaaccaggg aactattgat aacaaccacg
      181 tctaaactgc tccccaaata attttcaatt tccacggcat gatcaaaagc tgtcagcgaa
      241 tcggtctgcc ccagagtggt aaatttattt ataaaataaa cttttttcgc ttttgatttt
      301 ttaatggctt cagttacccc cgtcggtaaa aaatttggaa taagactggt gtataaatcg
      361 cccggaccga ttataatcaa atccgcctca acaatcgcct caacaacttc aggattaact
      421 ttaaccggct tgtcccgccg gctttgccgg cggggccgcg acaaataaac ttttttaatc
      481 ggacttcttt tgacgccagg ctcaacctca tcaatcttag tttcgcctgc tataatggtt
      541 ccgtctgtca attcagccgt taattccgcc cagtcaaatg aaaccggccc aacttttcct
      601 ttaactttta aaatttctga agctaacttt aaaacctgat tataatcttt taaattatca
      661 tataaattgg ccaaaaaaag attacccaaa ctgtggcctt taaaatctcc agccgcgaac
      721 cgccactgcc agatatcagc caaattggca ttatcagcca aagcaatcaa ggcatccctg
      781 acatcgccgc ttaaaacagt ctgatactga taacgaagct ggccgtaact gccgccgttg
      841 tcaaaagttg tcaccacggc cacgcagtca aaatcattct tcaggccggg caatatgttt
      901 ttggaaccgc ccccgccgcc gataaaaaca attttcttca tagtattata attgttatcc
      961 taatcaacca atcttatgcc aatcaacaaa tcactaatat ctttttacaa atatttgcag
     1021 taaaatattt gttattagtc aattcggata gaatttgtaa attcggataa cgcttaaact
     1081 atttgactgt aactgactta gctaaattgc ggggcttatc cggatcattt cctttgagca
     1141 ctcccagatg gtaagccagg atttggaccg gaataatatt gacgatcggc gacagcgggc
     1201 caacctccgg aactttgatc cagtaatcaa aaacatccga tgggaaaggg gaaacaccga
     1261 ttatgtaacc gcctcggctt ttgacttcct gggcgttgct taaaattgac tcccggtttt
     1321 catcattggc caccaaagta attaacggcg ttccttcgcc aatcaaagcg atcggcccgt
     1381 gcttcagctc gccgccggca aaaccttcgg cgtgaatata gctgacttcc tgaagtttaa
     1441 tggcgcactc cagagccatc ggataattca ctccccggcc gatgatatac atgctttcat
     1501 gtttgtgaat tttctcggcc agctgtctga tgtggtcttc atagcgggga ttaagcatgt
     1561 cattgacttt gccggccaaa gcgaccaaat tggttttccc ccgggccagc tggtcattaa
     1621 tagcgtaagc cagcaaagcc aggattgaca tctgagcggt ggtggctttg gtggaagcga
     1681 ccgcctgctc cggcccggcc ttgattaaca gggaataatc cgactgtctc attatggacg
     1741 acccctgaac attgaccagg gaaataattt taacgccttt ggctttggca atttccatcg
     1801 cttccaaaac atcggccgtc tcgccgcttt gggaaaccac gaccattaaa gattttggcg
     1861 ttaaaaaatg agaatagtta ataaattcag aggccggcac gaaattagtg tgctcaccgg
     1921 caatcttaga aaaaaaataa tcgccagtaa agcaaacttt gccggccgtg ccggccccga
     1981 ccagaaaagt cccaaaagca tttttaatgt ccctcgcaat tttcaatatt gctttttcat
     2041 cctgttccac cgccctcctg attgtttctt tctgttccat gatttcttta atcatgaaat
     2101 ggcggtaatc gcccttttca gcctgatcag cctgccagtc aatttcaatc agtcttttta
     2161 aaatttcctg gccggttttt aaactgaaaa aacgcgggct ctggtcaata acaaccagct
     2221 caccgtcatc caaataattg actctgcggg tataatttaa aaaagccgga atatcagacg
     2281 ccaaaaaata ttcattgtcg ccaataccga caatcagcgg cgaacccaac cgggcgccaa
     2341 caattaaatt attatcctga tgaatggcca caatggcgtt ccggccggtc agccggttaa
     2401 aagcttttcg gaccgcctga acaaaatcat tcttccggtt atattcctgg attaaattaa
     2461 ctatcacttc ggtgtcagtt tctgaattaa aaatgtaccc tttggcctgg agctcttttt
     2521 ttaaagtcaa ataattctca actatgccgt tctggacaag ggcgattttt ttattattgg
     2581 acaggtgggg atgggcgttg gcttcagtca ctttgccggt ggtcgcccag cgggaatgac
     2641 caatggcaat ctggtctccc tgacttttga aatccaaatc tttagcctcg atttcaccga
     2701 ctttgccgat ttttttcagc aaattaattt cctgatcttt cacggcaatg ccccaggagt
     2761 cgtacccccg gtattccaat ttttttaagc ctgccaaaat tatttttgga gctggctgtt
     2821 ggcctttgta ggatataatt ccgcacatga cattaagtaa aaaaataata aataaaaagt
     2881 tataagtttt tgttaattac atcagaaata gtctgtaagt ctgtcaatgt cttcaaattt
     2941 tcataatcat tagcctgata ttctttcccg tcttcccgtt taatagtcaa aattttcata
     3001 tcaggataaa ttttagccac ttcctgattt tccagcgggt tgtcattaat aaaaaagtta
     3061 actgaatctt tatccaagta atcggccagc agggaatatt ttttctgact ggtaaaaatt
     3121 gtcttgtcaa aatatttcat caggccggaa ttcaaaactt tgtctttttg aatattggga
     3181 tcgccccagg aaaccaggat cagccgggct ttattttgaa ataaatttaa aaaagaaacg
     3241 gcgtccaaat ataaataatc gctggttgac tgaattaatg cccgcagact gatcaacgct
     3301 ttattttcat caaaatcttt tttacttttt agagctttaa ttaatttata atcattccag
     3361 ccgtcttcag actgaacctg attataaatt tcaaaaaatt cctctttgcc aaaacctgta
     3421 tagctcaaaa catcataaag tttttcttta aaaagagtgg cgttaaataa agtatgatca
     3481 aagtctacaa agatggtcat attttttagt caacagtcat tagtcaacag tcactagggc
     3541 agggattagg ggtaagttat tagtaaatta ctaatgacta gtgactagtg gcttttctcg
     3601 caatctccag ctgttcaggc gtgttaatgc ctaaaccctc cagcggattg gcgcagctga
     3661 tagtttcaat ctgacagccc tggtcaaaag ctatttttat caaatcagtc aaataatatt
     3721 ctccctgctg attgttggtt gaaagcttat ccagattttt ccagagccag tcggcctcaa
     3781 aacaataata gctgggatta acctcagtga tggatttttg ctgatcagta gtatctttca
     3841 gttcaacaat ttcctgaata ttgccggcct gatccctaac cacccggcca aaatcataaa
     3901 acgtctgccg ccagtcattg taatccggca gtttaattgt aatcagagcc agcgtcggct
     3961 ttttttcccg aaaaacccga ttg
//
LOCUS       DASWHZ010000016         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1904173, whole genome shotgun sequence.
ACCESSION   DASWHZ010000016 DASWHZ010000000
VERSION     DASWHZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904173"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VGA49_00380"
     CDS             complement(<1..955)
                     /locus_tag="VGA49_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664258.1"
                     /translation="MAYKYYILFPCLLITALLSTTPAFADENICQKVCGADRYYPASS
                     LEECSDASQTFLCIQDKKPCFDYLIPRGWQTDSNCCCQDEPIEMPASGRINCQKFVGW
                     DINGFATEKNIFYPYGSSTKDPAECLQPAETTTPPPEEEPPSEKPEENGAKIVEEETS
                     LTTTGACPVFDLDGWFEPAQGVWQDDYKFKDKPNKQISFLLKPWPAYKAELDMVKDRD
                     SLLFGIFRAVGRDNQTERDFITLKGFTTGSKAIKVHYKFSLNDQTLYEDTDREFEILL
                     DGPCFLPQEFKAAIDASNGIPPTEQGPFIFKQAGPYLLTAEL"
     gene            1061..1567
                     /locus_tag="VGA49_00385"
     CDS             1061..1567
                     /locus_tag="VGA49_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664259.1"
                     /translation="MFNKKKIFEGANLFIGFFIALVLVTSLAYAYTLVEPGTVIDPPF
                     GPTEGNVTLEGIKGDPGAFTGSFEGDVSFGGNVAVGGALTVKGSPVAFKGNELYVCGA
                     TCTVRKVGSCNTSDVCRVTDPNPIKQPGQKCSGEQSAGNSKWFGLGCDYYTAACACDA
                     VLLGSLVK"
     gene            1640..2161
                     /locus_tag="VGA49_00390"
     CDS             1640..2161
                     /locus_tag="VGA49_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664260.1"
                     /translation="MNDKIKISNGVNQTVFNYIIVLFLIIFGVGLFLSYRQLSDKISQ
                     TQVVINITGSREEVAEKLEALIAENCTAENLTECTNLQILGYAVSTGDTRFCESITDQ
                     AFKNQCLDQPLFKQVLDRQDILLCDQALDAPVCRAAYNYDIAVKTGNRELCNQFSEDI
                     QARCLEAIATAGF"
     gene            2180..2950
                     /locus_tag="VGA49_00395"
     CDS             2180..2950
                     /locus_tag="VGA49_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9664261.1"
                     /translation="MVNIKNKIIFLGTGGGTGMVATQKLTTAGTWINIEGHNLYLDPG
                     PSCLYQINKFGLSAAGLQTVLVTHKHLDHVGELEALIDAMNFNGGRFKNQAIAVLKPK
                     DVLINNYHKKLVNKLVNVKANSRYKVRNLNVRTTKLLLEKPFYFGKLEEYGFVLEKDR
                     TKIAFIPETYWQKNLFKGIKADILVLNNLRDKKEDQKMIVATIKEINPKIVLMRHWIL
                     PVYEKGIKKFAGNIEKLTQIKTIAVQDGDIFDIKNLKI"
     gene            2962..3744
                     /locus_tag="VGA49_00400"
     CDS             2962..3744
                     /locus_tag="VGA49_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9664262.1"
                     /translation="MAKENDQNDKMKKNFYSSLSLLALVLVILAGVNIRNDWKAYDYI
                     GQSPEFKNQISITGQGKVMAIPDIASTVVGIRTENRQVAVAQKENAEKMNQLIKKIKE
                     AGVADEDLKTVNYNIFPIYDYLSDRGQVLRGYEVSQNLEVKIRDLEKIGQILAIAAAE
                     GANQVSGLNFTIDDEEVYRQQARELALANAREKAEALAKTVNIKLGKVVSFSETGSAP
                     PPAPLRAYAEFGVGGAADVALPEIEAGSQEIVIIATISFEIL"
     gene            3802..>3933
                     /gene="eno"
                     /locus_tag="VGA49_00405"
     CDS             3802..>3933
                     /gene="eno"
                     /locus_tag="VGA49_00405"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838494.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9664263.1"
                     /translation="MIIKEIRAREVLDSRGNPTVEVRVELTNGAAARASVPSGVSTGV
                     "
BASE COUNT         1150 a          760 c          919 g         1104 t
ORIGIN      
        1 ccagttccgc ggttaacagg taggggccgg cttgtttgaa aataaagggg ccttgttcgg
       61 tcgggggaat tccattagaa gcgtcaatgg cggctttaaa ttcctgcggc aggaaacaag
      121 gcccgtccag taatatttca aattctctat ccgtgtcttc atagagagtt tgatcgttca
      181 ggctgaattt ataatgaact ttgatggctt tggagccagt cgtaaagcct ttgagagtta
      241 taaaatctct ttcagtttga ttatcccggc caacagccct gaatattcca aaaagaaggg
      301 aatcacggtc tttgaccatg tctaattcgg ctttataagc tggccatggt ttcagcaaaa
      361 aggagatttg tttattgggt ttatctttga atttatagtc atcctgccaa actccctggg
      421 ccggctcaaa ccagccgtca agatcaaata ccggacaggc cccggtagta gtcaggctcg
      481 tttcctcctc cactattttt gccccgtttt cttcgggttt ttcagaaggg ggctcctctt
      541 caggcggagg cgtcgtcgtc tctgccggct gaaggcattc agctggatct ttggttgagc
      601 ttccatacgg atagaaaata tttttttccg tggcaaagcc attaatatcc caaccgacga
      661 atttttggca attgatccgg ccgctggccg gcatttcaat cggctcgtcc tgacagcagc
      721 aattgctgtc agtctgccag ccgcggggta tcaaataatc aaaacagggt tttttatcct
      781 gaatgcataa aaaagtctga ctggcgtcag aacattcttc cagggagctt gccggataat
      841 atctgtcagc tccgcagact ttctgacaga tattttcatc agcaaaagcc ggtgttgttg
      901 ataacaaggc agtgattaac agacaaggga acaaaatgta atatttatag gccataaatt
      961 taaaaatttt ttttagctgt atttatttta ccctaatttt gcttttttgg ggagttctct
     1021 gttataatta aataattata aacagcttca taataaacct atgttcaata agaaaaaaat
     1081 atttgaagga gccaatcttt tcattggttt ttttatcgct ctggttttgg taaccagtct
     1141 ggcttatgcg tatactctag ttgagccggg aacagtcatt gacccgcctt ttgggccgac
     1201 cgagggcaat gttactttgg agggaattaa gggggatccg ggagccttta ccggatcatt
     1261 tgaaggcgat gtcagtttcg gtggcaatgt ggcggtcggc ggggccttaa ccgttaaggg
     1321 cagtccagta gccttcaagg gcaatgaact ttatgtttgc ggggcgacct gcactgtccg
     1381 caaagttggc agttgtaata cctctgatgt ttgtcgagtg accgatccaa atccgattaa
     1441 gcagcccggg caaaaatgca gtggtgaaca atcagccggt aatagcaaat ggttcggctt
     1501 aggttgtgat tattacacgg ccgcctgtgc atgcgatgcc gttttactgg gatctttagt
     1561 taaataaaaa taaatttaca aatttatgaa ccctgttaga aattttactt taaattttta
     1621 atgggataaa taaaacatta tgaatgacaa aattaaaatt tctaacgggg tgaatcaaac
     1681 agtttttaat tatataatag ttctcttcct gataattttt ggtgttggtt tgtttctaag
     1741 ttaccggcaa ttgtccgaca aaatcagtca aacccaggtt gttattaata tcaccggatc
     1801 ccgggaggaa gtggctgaaa agctggaagc attgattgct gagaattgca ccgctgaaaa
     1861 tttaacggaa tgcactaacc ttcaaattct tggctacgcg gtttctaccg gcgacacccg
     1921 attttgtgaa tcaatcactg accaggcttt caaaaatcag tgccttgacc agccgctttt
     1981 taaacaagtt ttggacaggc aggatatttt attgtgcgat caggctttgg acgcacctgt
     2041 ctgccgggcc gcctacaact atgatattgc cgttaagacc gggaacagag aactctgcaa
     2101 tcagttttct gaagatatcc aggcccgctg tctggaagca attgccaccg ccggattcta
     2161 ggaaggttta tcttttctaa tggttaatat aaaaaacaag attatttttt tggggaccgg
     2221 cggcgggacc gggatggtgg ctactcaaaa gctaacgacg gccggtacct ggataaatat
     2281 tgaaggccat aatctttatt tagatccggg accaagttgt ttgtatcaga ttaacaagtt
     2341 tggtttgtca gccgctgggc ttcagacagt tttggtcacc cataaacatc tggatcacgt
     2401 cggggagctg gaagcgttga ttgacgccat gaatttcaac ggcggccggt ttaaaaatca
     2461 ggctattgcg gttttaaagc caaaagacgt cttgatcaat aattaccata aaaagcttgt
     2521 taataagctg gttaatgtca aggccaacag ccgttataag gttaggaacc ttaatgtcag
     2581 aacaaccaaa ctattgcttg aaaaaccttt ttattttggc aagctggaag aatacggctt
     2641 tgttctggag aaagaccgga ccaaaatcgc ctttattccc gaaacttact ggcaaaaaaa
     2701 tttgtttaaa ggaattaagg ccgacattct ggttttaaat aatttaagag ataaaaaaga
     2761 agatcagaaa atgattgtgg ccaccattaa agaaattaat ccaaaaatag ttttaatgcg
     2821 tcactggatt ttacctgttt atgaaaaagg aattaaaaag ttcgccggaa atattgaaaa
     2881 attaacccaa ataaaaacaa tcgctgttca ggacggcgat atttttgata taaaaaattt
     2941 aaaaatttaa aaagaaaaag tatggccaag gaaaatgatc aaaatgataa aatgaaaaaa
     3001 aatttttaca gttctttaag tttgctggct ttggttttag tgattttggc cggcgttaat
     3061 atccgcaatg actggaaggc atacgattat attggtcaaa gcccggaatt taaaaatcaa
     3121 atcagcatca ccggccaggg caaagtaatg gccattcctg atattgcttc aacagtcgtt
     3181 ggcattagaa ctgaaaacag gcaggtggcc gttgcccaga aagaaaacgc ggaaaagatg
     3241 aatcagctga ttaaaaaaat aaaagaggcc ggagtggctg atgaagacct gaaaacggta
     3301 aattataata tttttccaat ctatgattat ttatcggatc gcggccaggt cttaagaggt
     3361 tatgaagtct cgcagaatct ggaagttaaa atcagagact tggaaaaaat tggtcaaatt
     3421 ctggcgatcg ccgcggctga aggcgccaat caggtcagcg gactgaattt cacgatagac
     3481 gacgaggaag tttacagaca acaagcccgc gagctggctt tggccaacgc ccgggaaaaa
     3541 gccgaagccc tggccaaaac agtcaacatt aagctgggca aggtagtgtc ttttagcgaa
     3601 accggcagcg cgccgccacc ggcgccttta agagcttacg ccgaatttgg agttggcggc
     3661 gccgctgacg tcgctctgcc ggaaattgaa gccggcagtc aggaaatagt catcatcgcc
     3721 acgatttcat ttgaaatact ttagggtata gggtataggg gatagggtat agttatggaa
     3781 aattagttaa atgatttatc tatgattatt aaggaaataa gagccaggga agttttggat
     3841 tcaagaggca acccgactgt tgaggttagg gttgaattaa ctaatggagc ggctgccaga
     3901 gcgtcagtcc cctctggcgt ttccaccggc gtt
//
LOCUS       DASWHZ010000017         5427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_795327, whole genome shotgun sequence.
ACCESSION   DASWHZ010000017 DASWHZ010000000
VERSION     DASWHZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795327"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..459
                     /gene="rplN"
                     /locus_tag="VGA49_00410"
     CDS             88..459
                     /gene="rplN"
                     /locus_tag="VGA49_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9664264.1"
                     /translation="MVQPRSMLRVADNTGAKKLQCIRVLGGYRKRYGRIGDIITVSVK
                     EAAPHGMVKKGDVLHAVVVRSVKEIRRPDGTYLRFSENAAVIVDKKSKEPKGTRIFGP
                     VARELRAKGFSKIISLAPEVL"
     gene            464..775
                     /gene="rplX"
                     /locus_tag="VGA49_00415"
     CDS             464..775
                     /gene="rplX"
                     /locus_tag="VGA49_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9664265.1"
                     /translation="MKIKINDKVKIIKGKDRGKTGKVIQVFRKEGKVVVEGLNLLVKH
                     MRGRRQGEGGEKIQFNAPLAVENVMLICSKCNQPTRIGRKILENKKRSRLCKKCGEII
                     D"
     gene            778..1323
                     /gene="rplE"
                     /locus_tag="VGA49_00420"
     CDS             778..1323
                     /gene="rplE"
                     /locus_tag="VGA49_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9664266.1"
                     /translation="MINLEKKYQTEIVPRLKEELGYTNVMSVPKFEKVVINVGVGKNS
                     KDAGFIEMAEKNLTMLTGQKPIRTKAKKSISNFKIREGNIVGLKVTLRKKRMYDFLDK
                     LVNITLPRIRDFRGLSSKSFDGHGNFNIGFKEQIAFPEMGAETIEKLHGLEVSVSTTA
                     KNDQDAFELCRLAGFPFKKNN"
     gene            1332..1526
                     /locus_tag="VGA49_00425"
     CDS             1332..1526
                     /locus_tag="VGA49_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX9664267.1"
                     /translation="MATEAQIAKSKKSLKHPKYSSRVVRRCWRCGRKRAYMRKFELCR
                     ICFRELASKGEIPGIRKSSW"
     gene            1531..1968
                     /gene="rpsH"
                     /locus_tag="VGA49_00430"
     CDS             1531..1968
                     /gene="rpsH"
                     /locus_tag="VGA49_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9664268.1"
                     /translation="MYTTNDPISDMLTRIRNASAVKKTDIILPYSKIKMTIAELLERE
                     GYLNKAEKIEPVQDSKKMAKQDNLAKFQRIRLVLKYKDGQPAITWVKRVSKPGRRIYK
                     KKDELPIILNGLGLAIMSTPRGIMTNKEAKKAGQGGEFLFEVY"
     gene            1974..2525
                     /gene="rplF"
                     /locus_tag="VGA49_00435"
     CDS             1974..2525
                     /gene="rplF"
                     /locus_tag="VGA49_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9664269.1"
                     /translation="MSRIGKNPIEIPKGVEVRLEGSRIKVKGPKGELTQEIHPLVRIA
                     LADNQVKIDIDDKEDKQQKALWGLFGSLIRNMIVGVTEGYEKKLEINGVGYKAVVSGK
                     NVVFNLGYSHPVEFPIPDGISVEVDKNLVTVSGFDKQLVGEVAAQIRNLRKPEPYKGK
                     GIKYVDEVIRRKVGKALKGAEGA"
     gene            2532..2894
                     /gene="rplR"
                     /locus_tag="VGA49_00440"
     CDS             2532..2894
                     /gene="rplR"
                     /locus_tag="VGA49_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9664270.1"
                     /translation="MKSNRQKIKQQIRQRRHGRVRSKVFGTAQRPRLSVFRSLKYISG
                     QLIDDGRGETLASVFQHDLKQAKGAKTELAREVGKLLAQKAQAKGIKKVVFDRGPYKY
                     HGRVKALADGVREGGLEF"
     gene            2905..3459
                     /gene="rpsE"
                     /locus_tag="VGA49_00445"
     CDS             2905..3459
                     /gene="rpsE"
                     /locus_tag="VGA49_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9664271.1"
                     /translation="MDKKETQPTTKEDKAKSGSKTRARAPRRRGRRSDDDREKEEFEQ
                     KIIDLARVTRVMAGGKRMSFRACVALGDKNGRVGIGVAKGKDVTIAINKAVSQAKKKM
                     ISFSLVNEGTIPHQISSKFGAAKILLKPAPKGTGIIAGGVVRIVLDLAGVHNISGKII
                     GSKNKINNVKATIKALDSLKQVSR"
     gene            3465..3905
                     /gene="rplO"
                     /locus_tag="VGA49_00450"
     CDS             3465..3905
                     /gene="rplO"
                     /locus_tag="VGA49_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9664272.1"
                     /translation="MTLGLNDLKYKKAGKRRSKRVGRGNASGKGNYSTRGLKGQRARA
                     GGRGGLKLKGLRPRLLQIPKVRGFKSNKPKPAVVNVGDLNKIFKGNEMVTPRALMAKG
                     LVGQIQNGVKILGKGELDKKLTVVDCGFSKSAAEKIKAAGGVIK"
     gene            3911..5182
                     /gene="secY"
                     /locus_tag="VGA49_00455"
     CDS             3911..5182
                     /gene="secY"
                     /locus_tag="VGA49_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9664273.1"
                     /translation="MWEKIIRIWKIKDLRNSILFVLAMLVIFRLAANIPIPGIDREAL
                     RNLFSQNQFLGLINVFSGSAMSNFSVAALGVAPYITASIIFQLLVMIIPKLEELSKEE
                     AGQQKINQWTRYLSVPLAVLQSFGLITLLRQSSQQIITDLAAVQLTFIVLTMTAGTVF
                     LMWIGELISERKIGNGISLLIFAGIISALPTALQQTLVTFDYSQIFTLVIFVLVALVT
                     IVGVVIITEGERKVPVSYAKIVRGTRMYGGVDTYLPIKVNQGGVIPIIFAISIILFPP
                     MIAQFFLNAKTEFLVRAAEWVIMLFQNQLFYGIAYFLMVFAFTYFYTAVIFHPDQIAE
                     NLQKNGGFVPGIRPGKPTASYLQYTVNRIILTGAIFLGLIAVLPLAMQQLTGLQTLVI
                     GGTSLLIVVAVVIDSVQQINAQLTMRDYEGL"
     gene            5274..>5427
                     /locus_tag="VGA49_00460"
     CDS             5274..>5427
                     /locus_tag="VGA49_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX9664274.1"
                     /translation="MSLLLWILIFIVALAVLILAADFFTKHSEKVGMALGVPSFIIGV
                     TIVSIGT"
BASE COUNT         1579 a         1018 c         1374 g         1456 t
ORIGIN      
        1 gagaaaatat aagccactag tcactagtca ctagccacga gggttttata ctaatggcta
       61 atgactagtg actaatgact aactattatg gttcaaccac gatcaatgtt aagagtcgct
      121 gataatactg gagccaaaaa actgcagtgt attcgggtgc ttggcggcta ccgcaagcgc
      181 tacggccgga ttggtgatat tattacagtc agcgtcaaag aggccgctcc gcacggtatg
      241 gtcaaaaagg gcgatgtttt gcacgcggtg gttgtccggt ccgttaaaga aattcgccgg
      301 ccggacggga cctatttaag atttagcgag aacgccgccg tgattgttga taagaaaagc
      361 aaagagccga aaggcaccag aatttttgga ccggtcgccc gggaattaag agccaaaggt
      421 tttagtaaaa ttatttccct ggcgcctgaa gttttgtaat tatatgaaga ttaagattaa
      481 cgacaaagtt aagattatca aaggcaaaga ccggggcaaa accgggaagg tgattcaggt
      541 tttcagaaaa gaaggcaagg ttgttgtcga aggtcttaat ttactggtca agcatatgcg
      601 cggccggcgt cagggcgaag gcggcgagaa gattcagttt aacgcgccac tggccgttga
      661 gaacgtaatg ctgatctgtt ccaaatgcaa tcagccaacc aggattggcc gaaaaatttt
      721 agaaaataaa aaacgatcgc ggctttgtaa aaagtgcgga gaaattattg attaactatg
      781 attaatttag agaaaaaata tcagacagag atagttcccc ggttaaagga ggaactgggt
      841 tatacaaatg tgatgtcagt gccgaagttt gaaaaggtag tgattaatgt tggcgttggt
      901 aaaaattcca aggacgccgg ttttattgag atggcagaaa aaaatttgac catgctgacc
      961 ggccagaaac caatcaggac caaggccaaa aaatcaattt ctaattttaa aatcagggaa
     1021 ggcaatattg ttggtctgaa agttactctc cgaaagaaaa gaatgtatga ttttttggat
     1081 aagctggtta atattactct gccgcggatc agagactttc ggggcttatc ttctaaatcc
     1141 tttgacggtc atggtaattt caacattggc tttaaagaac agattgcttt tccggagatg
     1201 ggcgctgaaa caattgaaaa gcttcatggt ctggaagttt ctgtttcaac caccgctaaa
     1261 aatgatcagg acgcttttga gctgtgccgg ctggcgggtt ttccatttaa aaaaaataat
     1321 taattaaaat tatggctact gaagctcaga tcgcaaaatc aaaaaagtca ctgaaacacc
     1381 ccaaatattc aagccgggtg gtcaggcgct gctggcgctg cggccggaag cgggcctata
     1441 tgaggaaatt tgagctctgt cggatttgtt tcagagaact ggcctccaaa ggtgaaattc
     1501 ccggtattag aaaaagtagc tggtaaaatt atgtatacaa ccaacgatcc aatcagcgac
     1561 atgctgacca gaatcagaaa cgcctctgcc gttaaaaaaa cagatataat tttgccgtat
     1621 tcaaaaatta aaatgacgat tgctgaattg ttggagcggg aggggtattt aaacaaggcg
     1681 gaaaaaattg aaccggtcca ggattccaaa aaaatggcca aacaggataa tttggcgaaa
     1741 tttcagagaa tcagactggt tttgaaatat aaagacggcc agccggcaat tacctgggtt
     1801 aaaagagtca gtaaaccggg ccggcgaatc tataaaaaga aagatgaact gccgattatt
     1861 ctgaatggtc tgggattggc cattatgtct actccccggg gaattatgac caataaagaa
     1921 gccaaaaagg ctggccaggg cggggaattt ttgttcgaag tgtactaaaa actatgtcac
     1981 gaataggcaa aaatccaatt gaaattccaa aaggggttga agtcaggctg gaaggttcgc
     2041 gaattaaggt taagggacct aaaggcgagc tgacccagga gattcatcct ctggttagga
     2101 ttgctctggc tgataatcag gtgaaaattg acattgacga taaagaagat aagcagcaga
     2161 aagctttgtg gggattgttt gggagtttaa tccggaacat gattgtcggg gtaactgaag
     2221 gctatgaaaa gaagctggaa atcaacggag ttggctataa ggcggtcgtt agcggtaaaa
     2281 acgttgtttt taatctgggt tattctcatc cggttgaatt tcccattcct gacggcatca
     2341 gcgttgaagt tgataaaaat ttggtgacag tttcaggttt tgataaacag ttagtcggag
     2401 aggtggccgc tcaaatcaga aatctaagaa aacccgaacc ttataaaggc aaaggtatta
     2461 aatatgttga tgaagttatc agacgcaagg ttggcaaggc tttgaaagga gccgaaggcg
     2521 cctaaaatat tatgaaaagc aaccgacaaa aaattaaaca gcagattagg cagcggcgtc
     2581 atggccgggt tcgatccaaa gtttttggca cggctcagcg tccacgtttg tcagttttca
     2641 gaagcctgaa atatatttca ggccagctga ttgatgatgg tcgcggcgaa actttagcgt
     2701 ctgtttttca gcatgacctg aaacaggcta aaggagccaa aactgaactg gcccgggagg
     2761 ttggcaaact gctggcccaa aaagcccagg ccaaaggaat taaaaaagta gtttttgaca
     2821 gaggcccata caaatatcat ggccgggtta aagccctggc tgacggcgtc agagaaggag
     2881 gcctggaatt ttaattttaa taatatggat aaaaaagaga cacaaccgac aactaaagaa
     2941 gataaggcta aatcagggtc aaaaacgagg gccagggcgc cccggaggcg gggccggcgg
     3001 tctgacgatg accgcgaaaa agaagaattt gaacaaaaaa ttattgattt ggcccgggta
     3061 acccgggtga tggccggcgg caagcgaatg agcttccggg cctgcgtggc tttaggagac
     3121 aagaatggcc gggttggtat tggcgtggcc aaaggcaagg acgtcaccat cgccattaac
     3181 aaagctgtca gccaggccaa gaaaaaaatg attagttttt cgctggttaa cgagggaaca
     3241 attcctcatc agatcagcag taagtttggc gcggctaaaa ttttattaaa accagctccc
     3301 aaaggaacag ggattattgc cggcggcgtt gtccggattg ttcttgattt ggccggcgtt
     3361 cacaatattt caggaaaaat tattggttcc aaaaataaaa ttaataatgt taaggcgacc
     3421 attaaagctt tggacagctt aaagcaggtt agccggtaaa gattatgact ttaggtttaa
     3481 atgatttaaa atacaagaaa gccggtaaac gccggtcaaa gagggttggc cgggggaatg
     3541 cttccggcaa aggcaactat tccacccgcg ggctgaaagg ccagcgagcc cgggcaggcg
     3601 gtcggggcgg tttgaagctg aaaggcctgc gtccccggtt gctgcaaatt ccgaaagtcc
     3661 gcggctttaa aagcaataag cccaagccgg ccgttgttaa tgttggtgat ttgaataaaa
     3721 tttttaaagg taatgaaatg gtaacaccca gggctttaat ggccaaagga ttagttggtc
     3781 agattcagaa cggcgtaaaa attttgggta aaggcgagct ggataaaaaa ttaacagtcg
     3841 ttgactgcgg tttttccaaa tcagccgctg aaaaaattaa agcggccggc ggcgttatta
     3901 aataattcct atgtgggaaa aaattatcag gatctggaaa attaaagatt tgcgcaacag
     3961 tattttgttc gtgctggcga tgctggttat tttcaggctg gcggctaaca ttcccattcc
     4021 aggcattgac cgggaagctt taagaaattt attttctcaa aatcagtttt tgggcctgat
     4081 taatgttttt tccggcagcg ccatgtccaa tttttcagta gccgccctgg gcgtggctcc
     4141 gtatattacc gcttcaatta tttttcagct gctggtgatg attattccca agctggaaga
     4201 attgtccaaa gaagaagccg gccagcaaaa aattaatcag tggacccggt atttgagcgt
     4261 tccactggcg gttttacagt catttggttt gattactctc ttaaggcagt cttcccagca
     4321 gatcattact gatttagcgg ccgttcagct cacttttatt gttttaacca tgaccgctgg
     4381 tactgttttt ttaatgtgga ttggtgaact gatttcagaa agaaaaatcg gcaatggtat
     4441 ttctttgctg atttttgccg gtatcatctc cgccttgccg accgctttac agcagaccct
     4501 ggtaacattt gattattccc agatttttac tctggtgatt tttgttttag tggctttggt
     4561 aacgattgtc ggcgtggtca ttattactga aggggagagg aaagttccgg tttcttacgc
     4621 taaaatcgtc cggggaacca gaatgtacgg cggagttgat acttatctgc cgattaaagt
     4681 taaccagggc ggagtcatcc cgattatttt tgccatttcc ataattttat ttccgccaat
     4741 gattgcccag ttttttttaa acgccaaaac tgaattttta gtcagggcgg ctgaatgggt
     4801 gattatgctt tttcaaaatc agttatttta cggtattgcc tattttctga tggtttttgc
     4861 ctttacttat ttttacacgg ccgttatttt tcatcctgat cagatcgctg aaaatttgca
     4921 aaagaacggc ggctttgtcc cgggtatcag gccgggcaag ccgaccgcca gttatctgca
     4981 atataccgtt aaccggatta ttttaaccgg ggcgattttc ctcgggctga tcgctgtttt
     5041 accgttggcg atgcagcagc tgaccggtct gcagaccctg gtcattggcg gcaccagtct
     5101 gctgatcgtg gtagccgtgg tgattgattc agttcagcag ataaacgccc agctgacaat
     5161 gcgtgattat gaggggcttt gataaaaggg ttaagtgggt taagcgggat aagggggata
     5221 agcgggttaa gtgtcctctt taaccccttt atcccgttta ttcccttatt tccatgtctt
     5281 tattactttg gattttaatt tttattgtcg ccttggcggt tttgattttg gcggctgatt
     5341 tttttacgaa acattctgaa aaagtaggca tggctctggg cgtgccttct tttattattg
     5401 gggtgacgat tgtcagcatt ggcactt
//
LOCUS       DASWHZ010000018         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3015375, whole genome shotgun sequence.
ACCESSION   DASWHZ010000018 DASWHZ010000000
VERSION     DASWHZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015375"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VGA49_00465"
     CDS             <1..527
                     /locus_tag="VGA49_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664275.1"
                     /translation="EQNATIAAYVSFLNSFEFPIQIVIQSRKLNIDPYIDRLKMLEKE
                     QTNELLKIQINEYRNYVSELIELGEIMSKKFYVVVPYHPLTAKQKNFFKRLGEALSPA
                     KTVALKRKRFLERREDMMKRVDLVMSGLSSIGLNSVVLGTQNLIELYYNTYNPTTAEQ
                     EKLADMDKLRVEGK"
     gene            564..2405
                     /locus_tag="VGA49_00470"
     CDS             564..2405
                     /locus_tag="VGA49_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9664276.1"
                     /translation="MPEEKPKKTTPEKAAKEKEILEAEKIYRKGVVSVRDLIAPASFK
                     VNSTFLELGTKFVRTIFIISYPRYIAVGWFAPVINFSATIDIGMFFYPVKSAIILKQL
                     KKKVGELEAQIIGDSEKGAPRDPIRETALKDIEALRDSLTQGTEKFFQFALYVTLYAD
                     SKEELDKMTDKIELMFGSRLVYSKKVLYQAEQGFNSTMPLGNDELMITFNMNSSPCAS
                     SFPFISAELTSDNGVLYGINRHNNSLILFDRFSLQNANFTVFATSGAGKSYAIKLEVL
                     RSMMFGTDVIIIDPEMEYKHLSDAVAGTYINISLASESKINPFDLPRPVGGEVKTADI
                     IRSAVITLKGLVRLMLGELNPQEDSIVDRALLETYAKKDITADADLSKVEPPLMQDFQ
                     EVLDGMEGGAELSERLKKYTEGTFSGLFNSPTNVDTRNQLVVFSVRDLEDELRPIAIY
                     TIVNYIWNVVRSELKKRILVIDEAWWLMQREDSAKFIYALVKRCRKYYLGVTTITQDV
                     NDFLGSPYGQAILNNSSLQLLMKQTPAAIDLIQKTFMLTEGEKYLLLECGVGEGIFFA
                     GSKHAAIKVVASYTEDQLITSDPRQLLEIEDAKKAFEEESAAGEMQI"
     gene            2410..3024
                     /locus_tag="VGA49_00475"
     CDS             2410..3024
                     /locus_tag="VGA49_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664277.1"
                     /translation="MDRRTIILIAVAGVLVISLLVIGLYAIFRSPEPVQAPEANLNTS
                     NANQPTTGLATNQPPVTFELPQPVEPVVVDQATKDKADIERLARTFTERYGSFSNQTD
                     FENLEDLKIFMTASMRAYVDSFIARQRAQRGDTSIYYGVTTKVLTTEITDYSPSLGRA
                     EVLVTVQKQESTGSTTNTKVFNQEAALDFARDNGSWKLEGLYWK"
     gene            3323..4096
                     /locus_tag="VGA49_00480"
     CDS             3323..4096
                     /locus_tag="VGA49_00480"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9664278.1"
                     /translation="MLKFLLDILFPVFCLGCRTEGLWLCQNCQKQIRFNRHFICPICK
                     KNSDFGQTCQKCRLENSLDGCLVALSYEQELVRELIRVFKYKYILDLKEILSGFLIDF
                     LKKVQNKKIGFQYIIKKDGSAEAVAGQVKVPEIFLKADQAILIPVPLHKKRLADRGYN
                     QSDLLAEDLSKSFGFELNKNLLVRHKNTRAQARLKKEERLVNVVDAFECPRPEAVKGL
                     NIILVDDIYTTGATIGECAKVLKEAGAGEVWGIVLARGQ"
     gene            4074..4562
                     /locus_tag="VGA49_00485"
     CDS             4074..4562
                     /locus_tag="VGA49_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9664279.1"
                     /translation="MSWRGVNKALKQESIKTRKQGIIKALKQESIKARKQESIKYMSD
                     NFKDKLRKKMDEYVHFIYDITKNFPKEELYGVTSQLRRAALSIVLNYIEGYARMKPAV
                     QLNFFEISYASFKESKYLSHFSFTRNYLSKSDHKICNFFEEEIGAMLWTEIDSLRKSL
                     HK"
     gene            4667..5107
                     /locus_tag="VGA49_00490"
     CDS             4667..5107
                     /locus_tag="VGA49_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9664280.1"
                     /translation="MTPKKGFTLVELLVVIAIIGLLSTLSVIALGSARQKARDALRLS
                     HIKQIQTALEFYFSDYNNYPVGQELVLGLAGGACLDETGFTSAETCANPYMGSVPPNP
                     APGGADYIYSQTADGAGYQLIFNLEDDSGGLSAGQRQATQEGIN"
     gene            complement(5177..5584)
                     /locus_tag="VGA49_00495"
     CDS             complement(5177..5584)
                     /locus_tag="VGA49_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9664281.1"
                     /translation="MSQTTYKKILIVEDEPFLLDVYKFAFECAGYQVLTAQSRGKALE
                     IIKTSRPDLILLDVIIPEKEEGLVDYTAKEGFQLLRELKKDNLKEIPVIVFSNLDYDH
                     SAESTFKEMNVLDYIIKANFSPKQLVSRVGQLV"
BASE COUNT         1687 a         1129 c         1273 g         1646 t
ORIGIN      
        1 acgagcagaa cgccaccatt gccgcttatg tcagtttttt aaattctttt gaatttccga
       61 tccagatagt gattcagtcc agaaaactga acattgatcc gtatattgat cgtttaaaaa
      121 tgctggagaa agaacaaacc aatgaactgt taaaaatcca gattaatgaa tatcggaatt
      181 atgtttctga actgatcgag ctgggcgaaa ttatgagcaa gaaattttac gtggtcgtgc
      241 cctaccatcc gctgaccgcc aaacagaaaa atttttttaa aagactgggc gaggccttat
      301 cgccggccaa aacagtcgct ctgaaaagaa aaagattttt ggagagaagg gaagatatga
      361 tgaagcgggt tgatctggta atgtccgggt tgtccagcat tggtttaaat tcagtggttc
      421 tgggcaccca gaatttaatt gagctttatt acaataccta taatccgacc acggctgaac
      481 aggaaaaact ggctgacatg gataaattaa gagtagaagg caaataacta taccctatac
      541 cctatcacct atcccctagt aatatgcccg aagaaaaacc caaaaaaaca acgccggaaa
      601 aagccgctaa agaaaaagaa atcttggagg cggaaaaaat ttaccggaaa ggagttgtca
      661 gcgtccggga tttgatcgcc ccggcttctt ttaaagtcaa ttcaactttt ctggaactgg
      721 gcactaagtt cgtccggaca atttttatta tttcttatcc gcgttatatt gccgttggct
      781 ggttcgcccc ggtgattaat ttcagcgcca cgattgatat tggcatgttt ttctatccgg
      841 ttaagtcagc cattatttta aaacagctta agaaaaaagt cggcgagctg gaagcccaga
      901 ttatcggcga ttctgaaaaa ggcgcgccgc gcgacccgat ccgggaaacc gctttgaaag
      961 acattgaagc tttgcgggac agtctgaccc agggaactga aaaatttttc cagtttgcct
     1021 tgtatgtcac cctttacgct gattccaaag aagagctgga taaaatgacc gataaaatag
     1081 agctgatgtt tggctcccgg ctggtttatt ccaaaaaggt tttatatcag gccgagcagg
     1141 gctttaattc aaccatgccg ctgggcaatg acgagctgat gattactttt aacatgaatt
     1201 catcgccctg cgcctcgtct tttcctttta tttcggccga gctgacttct gacaacggcg
     1261 ttttgtacgg cattaaccgc cataacaatt ctttgattct gtttgaccgg ttctctctgc
     1321 aaaacgccaa ctttactgtt ttcgccactt ccggggccgg taaaagctac gctatcaaac
     1381 tggaggtttt acggagcatg atgtttggca ccgacgtgat tattattgat ccggaaatgg
     1441 aatacaagca tttgtctgac gctgtcgccg gcacatatat taatatttct ctggcctcgg
     1501 aaagtaaaat taacccgttt gatctacccc ggccggttgg cggcgaagtt aagaccgctg
     1561 acattatccg gtcggccgtg attactttga aaggcctggt ccgtttaatg ctgggcgagc
     1621 tcaatcccca ggaggattca attgttgacc gggctttgct ggagacttac gccaaaaaag
     1681 atattacggc tgacgctgat ttatcaaagg ttgagccgcc gctgatgcag gattttcagg
     1741 aagttttgga cggcatggag ggcggggcgg aattaagcga acgtctcaaa aaatatactg
     1801 aaggaacgtt ttcgggttta ttcaacagcc cgaccaatgt tgacaccaga aatcagctgg
     1861 tggtctttag cgtccgcgat ctggaagatg aattaaggcc aatcgcgatt tataccattg
     1921 tcaattacat ctggaatgtt gtccggtcag agctgaaaaa aaggattctg gtgattgacg
     1981 aagcctggtg gctgatgcaa agagaagatt cagccaaatt tatttacgct ctggtcaagc
     2041 gttgtcggaa atattatctg ggcgtaacga cgattaccca ggatgttaac gactttttgg
     2101 ggtctcctta tggccaggct attttgaata actcatcttt acaattattg atgaagcaaa
     2161 caccagcagc cattgattta atccaaaaaa cttttatgct gaccgagggc gagaaatatc
     2221 ttctgctgga gtgcggcgtt ggcgaaggca tttttttcgc cggatcaaag cacgccgcca
     2281 ttaaagtcgt cgcgtcttac actgaagatc agctgattac ttccgatccc cgccagctac
     2341 tggaaattga ggacgccaaa aaagcttttg aagaagaatc agccgccggc gagatgcaaa
     2401 tttaaaatta tggaccgaag aacaattatt ttaatagctg ttgccggagt cctggttatt
     2461 agtttgctgg tcatcggcct ttacgctatt ttcaggtcgc ccgagccggt tcaggctccg
     2521 gaagctaatt taaatacatc caacgctaat cagccaacaa ccggattggc caccaaccag
     2581 ccgccggtca cttttgaact gccccagccg gttgagccgg tagttgtgga ccaggcgacc
     2641 aaagacaaag ctgacattga acggctggcc cggactttta cggaacgcta cggcagtttt
     2701 tccaatcaga ctgattttga aaatctggaa gatctcaaaa tttttatgac cgcttccatg
     2761 cgggcttatg ttgacagctt tatcgcccgg caaagagccc agcggggaga cacttccatt
     2821 tattacggcg tgaccaccaa agttttaacg actgaaatta ccgattacag tcccagcctg
     2881 ggacgggccg aagtcctggt gaccgttcaa aaacaggaat caaccggctc cacgactaat
     2941 accaaagttt ttaatcagga agcagcacta gatttcgcca gggacaatgg atcatggaag
     3001 ttagagggac tttactggaa gtaaaattat aaattcaaaa ttcgaaatcc taatatctaa
     3061 taatataaat cctaaattag aaatcctaaa tcctaaatcc cgatccgcta attttacctt
     3121 cggtaaaatt ttagcggatc gtggatccac gacgaagtcg ggataaatta taaatcataa
     3181 tgttttaaat actaaactat tttattaagc tgtttgaatc attgtgattt agaatttaaa
     3241 atttgtttag aatttcgata ttagatatta gaatttaaaa agaaccactt actatttcca
     3301 ccccccgcta attggggttt ttatgttgaa atttttatta gatatattat ttcctgtctt
     3361 ctgcctgggc tgccggaccg agggtctttg gctgtgtcaa aattgtcaga agcagattag
     3421 atttaatcgt cattttattt gtccaatttg taaaaaaaat tctgattttg ggcagacttg
     3481 tcaaaagtgc cgccttgaaa attctttaga cggctgtctg gtggccttgt cttatgagca
     3541 ggaactagtc cgggaactga tcagggtttt taaatataaa tatattttgg atttaaagga
     3601 aattttgagc ggatttctaa ttgatttttt gaaaaaagtt cagaataaaa aaattggttt
     3661 tcagtatatc ataaaaaaag acggttcagc cgaagccgtc gccggtcagg tcaaagttcc
     3721 ggaaattttt ttaaaggcgg atcaggcaat tttgattccg gtgccgcttc ataaaaagcg
     3781 gctggctgac cggggatata atcagtctga tttattggct gaagacttgt ctaaaagttt
     3841 tggttttgag ctgaataaaa atttattggt tagacataaa aatacccggg cccaggcccg
     3901 gctcaagaaa gaagagcggc tggtcaatgt tgttgacgct tttgaatgtc cgcgacccga
     3961 agccgtcaaa ggtttaaata taattttagt ggatgatatt tatactaccg gcgcgacaat
     4021 cggggaatgc gccaaagttt taaaagaggc aggcgccggc gaagtctggg gaattgtcct
     4081 ggcgcggggt caataaagca ttaaagcaag aaagcattaa aacaagaaaa caaggaatca
     4141 ttaaagcatt aaagcaagaa agcattaaag caagaaaaca agaaagcatt aaatatatga
     4201 gtgataattt taaggacaaa ttaagaaaga agatggatga atatgttcat tttatttatg
     4261 acattactaa aaattttcca aaagaagaac tttatggtgt aacatcacag ttaaggagag
     4321 ccgcactttc aatagtgtta aattatattg aagggtacgc tcgaatgaaa ccagcagttc
     4381 agttgaattt ttttgaaata tcatatgctt catttaagga atctaaatat ttatcacatt
     4441 tttcttttac aagaaattat ttgtcaaaat ctgatcataa aatttgtaat ttttttgagg
     4501 aagaaatagg cgcgatgctt tggacagaaa ttgactcttt gcgaaagagc ttacataagt
     4561 agttgttttt atgtttttat gctttaatgt tttcatgaat tcttgatttt ctgaaagctt
     4621 attgatatac taagctaaac ttgttaataa attaaatttt aataaaatga cacctaaaaa
     4681 agggtttacg ttggtagaat tactggtggt tatcgccatt attggtttgt tgtcaacttt
     4741 gtcggtgatc gctttgggtt cggcccggca aaaagcccgg gacgccctga ggctgtctca
     4801 tatcaagcag attcagacag ctttggagtt ttattttagc gattataata attatccggt
     4861 cggtcaggag ctggttttgg gtctggccgg cggcgcctgt ctggacgaga ctggctttac
     4921 ttcagcagaa acctgcgcta acccctacat gggttcagtc ccgcctaacc ccgcgcctgg
     4981 gggagctgat tatatttaca gccagacagc tgacggcgcc ggttatcagc ttatatttaa
     5041 tctggaggat gacagcggcg gcttatccgc cggccagcgc caggcaaccc aggaagggat
     5101 aaactaaaaa aaatttacag attaaattaa aaaaccctgt ctgaaggcgg ggtttttggc
     5161 ttgctcaaaa ttaagtttag accagctggc caacccggct gaccagttgt ttgggcgaaa
     5221 aattagcttt gataatatag tccaaaacat tcatttcttt aaaagttgat tcagcgctat
     5281 ggtcatagtc cagattggaa aagacaatga ccggaatttc ttttaaatta tcttttttta
     5341 gttcccgcag cagctggaaa ccttccttgg cggtataatc aaccagacct tcttcttttt
     5401 cagggataat aacatcaagt aaaatcagat caggccggct ggttttaatg atttccaggg
     5461 ctttgccgcg gctttgagcg gtcagaacct gatagccggc gcactcaaaa gcaaacttgt
     5521 agacatcaag caggaatggt tcgtcctcaa ctatcagtat ttttttgtaa gtggtttgtg
     5581 acattgtttc attttgaata ttatatgcta ccacgttctt taaaaactgg caagtaaacg
     5641 ttaaacgtac tgcctttatc ttcaccttca ctaaaaattt ccagacggga gttattggcg
     5701 tcaagaattt gtttggtgat atacagaccc aggcc
//
LOCUS       DASWHZ010000019         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1533451, whole genome shotgun sequence.
ACCESSION   DASWHZ010000019 DASWHZ010000000
VERSION     DASWHZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533451"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..401
                     /gene="cutA"
                     /locus_tag="VGA49_00500"
     CDS             96..401
                     /gene="cutA"
                     /locus_tag="VGA49_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HEX9664282.1"
                     /translation="MLVEISVTCKNTAEARKIARQLLTNKLVACANFWPVNSIFTWHG
                     KQVNGREVLLVCKTESRHARRAKLLIQDLHSYELPTLTVSNITTTPAVTRWVKEVTK"
     gene            413..841
                     /locus_tag="VGA49_00505"
     CDS             413..841
                     /locus_tag="VGA49_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664283.1"
                     /translation="MARKLGYALIYYFLAVLAFLAIDYALVDQMFKQAGWIILAGGGS
                     FLIARTYFHKTPGDAKAGAELAILWIGLGVVLDFLLGWLALMAGEQASYDLLLNLYTS
                     LFFWAGLGVSFLGITFAGHFTHGGALMKIPPPAPGKNIKK"
     gene            complement(954..>1889)
                     /locus_tag="VGA49_00510"
     CDS             complement(954..>1889)
                     /locus_tag="VGA49_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX9664284.1"
                     /translation="NSKSAGMTKSTAQIIDGKALAELIFEQLSKTVARLPHRPSLAAI
                     MIGDDKASQLFIKRKKLACRKVGITFHEYQCGNDKCLPNITADGVLEMIDWLNNDDQV
                     DAIIVQLPVPKKFDTETIIQRISPAKDVDGFHAENTKKLLGNTQEITPPLILAIQAAI
                     GFSDKLPENQTVIIISKNPIFSQPLARVLQRQGFRLNTVLPDDPNVKTAAAAADVVIS
                     VVGKKYFVKADMVKPDALVIDVGTTLEDNNGTSKLYGDVDPGVKKVAGWLTPVPGGIG
                     PLTVAMLLKNVVELAAKKQGSLQPHLTPPSKGGEN"
BASE COUNT          423 a          433 c          481 g          552 t
ORIGIN      
        1 gatgaccctg ccaaccaatg cgaaagtttt cggcgcgggg agactcatca ttttgtggaa
       61 gttgacggca attgcaatat aattaaggcg cagtaatgtt ggttgaaatt tctgtcactt
      121 gcaaaaatac ggctgaagcc cgaaaaatcg ccaggcagct gctgaccaac aagttagtcg
      181 cctgtgccaa tttttggccg gtcaactcga tttttacgtg gcatggcaaa caggttaatg
      241 gccgagaagt cctcttagtt tgcaaaacag aatcccggca tgctcggagg gctaagttgt
      301 taatccagga cctccatagt tatgaactgc caacgcttac cgtttctaat atcaccacta
      361 ccccagcggt gacccggtgg gttaaagaag ttactaaata attttcaaat ctatggctcg
      421 caaacttggc tacgcgctta tatattattt tctggcggtc ttggctttct tagccatcga
      481 ttacgccttg gtagatcaaa tgtttaaaca ggccggctgg attattttgg ccggtggcgg
      541 cagttttttg atcgctcgga cgtattttca caaaactccc ggggacgcca aggccggcgc
      601 cgagctggct attttatgga ttggcttggg cgtagttttg gattttctac ttggctggtt
      661 ggctttgatg gccggagaac aagcttcgta tgatttactt ttgaatttat acacctcgct
      721 ttttttctgg gcgggattgg gcgtgtcatt tttgggcatt acctttgccg gccacttcac
      781 ccacggtggg gctttaatga aaattccgcc accggctcct ggaaaaaata taaaaaaata
      841 ggtgggtttg aaaaattttc cggctggagg tgggcgtctt agctgcaccc cacctatcct
      901 ccccttagca aggggaggga gtcttggaaa ggctttctgc gaacaatccc tgtttagttt
      961 tcccctcctt ttgaaggagg ggttaggtgt ggttgcaatg agccttgttt ctttgcggcc
     1021 agttcaacca cgtttttgag cagcatggcc acggtcaacg gaccgatgcc gccgggtacg
     1081 ggagttagcc agcccgcgac ttttttaacg ccggggtcaa catcgccgta gagtttagat
     1141 gttccgttat tatcttctaa cgtagttcca acgtcaatca caagcgcgtc gggcttaacc
     1201 atgtcggctt tgacaaagta ttttttgccg accacgctaa tgaccacgtc ggcggccgcg
     1261 gccgcggttt ttacgtttgg gtcatcgggc agcaccgtgt ttaaccggaa gccctggcgt
     1321 tgcaggacgc gcgctaaagg ctgactgaaa attggatttt ttgaaatgat aatcacggtt
     1381 tgattttctg gcagtttgtc tgaaaatccg atcgcggctt gaattgccag gattaaaggc
     1441 ggagtaattt cttgcgtatt accgagtagt tttttggtat tttcagcatg aaatccgtct
     1501 acatctttgg ccgggctaat acgctggatt atcgtttcag tgtcaaattt ttttggaaca
     1561 ggcagctgaa caataatcgc gtccacttga tcgtcattat tcagccagtc aatcatttcc
     1621 agtacaccgt ctgcggttat gttgggcagg catttgtcgt tgccgcactg atattcatga
     1681 aacgtgatgc cgacttttcg gcaggccaat tttttccgct taataaaaag ctgcgaggct
     1741 ttatcgtcgc caatcataat cgccgccagt gacgggcggt gcggcagcct ggccactgtc
     1801 ttactgagct gttcaaaaat cagctcagcc agcgctttgc catcaataat ttgggcggtt
     1861 gattttgtca tgccggcaga cttggaatt
//
LOCUS       DASWHZ010000020         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_57680, whole genome shotgun sequence.
ACCESSION   DASWHZ010000020 DASWHZ010000000
VERSION     DASWHZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57680"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VGA49_00515"
     CDS             complement(<1..1102)
                     /locus_tag="VGA49_00515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9664285.1"
                     /translation="MSPNKSEIKSGAESELKIGFEPLADSDFDSIFGEIEDHLAKEDF
                     KEAHTQLVDMAEMMAGKIKSKTKEPVSDLEKTEAMGSFRGLLRAISVDALDNIEAISE
                     AETAEELVSVENKIKKPRFSDRLEKLLKSGHLEVEKKLGAGGMGEVYAAEHEYLGPRA
                     VKILQMKDSLGRDISVSPTVLEKLRQEMKILAKLDHPNIVKIFDANINEAGAYIALEK
                     VDGIDLREYIKPVITGTFPVDKALEMFEGIAKAVQYIKEQNFVHRDIKPANILLPEDP
                     ETKTIEFQKAKLADFGLSEVKKDIPDLEPKGYKEGTPLYSSPEQFLGKDLDTKSDVYS
                     LGATMYEVLTGKKPIEAKTIPEMTTRVRFEKPK"
     gene            complement(1107..1307)
                     /locus_tag="VGA49_00520"
     CDS             complement(1107..1307)
                     /locus_tag="VGA49_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664286.1"
                     /translation="MPSEKKQKKEKELLQKKLDLLRKIYDDYNRFLASFNKAKKKIIQ
                     ETVAKKEKDRLNKIKEIKEKIK"
     gene            complement(1351..1758)
                     /locus_tag="VGA49_00525"
     CDS             complement(1351..1758)
                     /locus_tag="VGA49_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664287.1"
                     /translation="MCRIQDEVQSELKNINTTNLEQELQEVSSESLADRATELSSGRN
                     MKDLKKELFWIDVEIQGKMPTWRHWEGCVKEDPEILTAKPEFADDYLMLKHSLERLTE
                     VRTGLLNRIKLLKAISAVMRRRVDEEQKIEEAA"
BASE COUNT          491 a          479 c          308 g          707 t
ORIGIN      
        1 tttttggttt ttcaaatctg actctggtag tcatttcggg gattgtttta gcttcaattg
       61 gtttcttacc ggttaaaact tcatacattg tggctcccaa agaataaacg tctgatttgg
      121 tgtcaagatc ttttcccaaa aactgttctg gcgaagaata tagaggcgtc ccttccttat
      181 acccctttgg ttcaagatca ggaatatcct ttttaacttc ggacagacca aaatcagcca
      241 gtttagcttt ttggaattct attgttttag tttcaggatc ttctggcagt aaaatattgg
      301 ctggcttaat atcgcggtga acaaaatttt gttcttttat atattggaca gctttggcaa
      361 taccctcaaa catttccaaa gctttgtcaa ctgggaatgt tccggtaata acaggtttaa
      421 tatattccct taaatcaatg ccatcaactt tttctaacgc aatgtaggcg ccggcttcat
      481 tgatattagc gtcaaaaatt ttaacaatat tgggatgatc cagtttagct aaaattttca
      541 tttcttgtct tagcttctcc aaaactgtcg gactgacgct aatgtcccgg cctaatgaat
      601 ctttcatttg taatatttta acggctcttg gtcccaaata ttcatgttcg gccgcgtaga
      661 cttcgcccat gcctccggcg ccaagttttt tttcaacttc taaatggccg ctcttcaata
      721 atttttccag ccgatcgcta aatcgtggct ttttaatctt gttttcaact gaaactaact
      781 cttccgctgt ttcagcttct gaaattgctt ctatattatc aagcgcgtca acagaaattg
      841 ctcttaataa tcccctgaaa gaacccatgg cttctgtttt ttcaagatca ctgaccggct
      901 ctttagtttt tgacttaatt tttccagcca tcatttcagc catatcaacc aactgggtat
      961 gagcctcttt aaaatcttct tttgccagat gatcctcaat ctccccgaaa atgctgtcaa
     1021 aatcgctatc agccaaaggc tcaaaaccga tttttaattc tgattcagcc cctgatttaa
     1081 tttcactttt gttaggagac ataaaattat ttaatctttt ccttgatttc ctttatttta
     1141 ttcagcctgt ctttttcttt ttttgcgacg gtctcttgaa taattttttt tttagctttg
     1201 ttaaaactgg ctaaaaaccg attataatca tcataaattt ttctcaaaag atcaagtttt
     1261 ttttgtaata gctctttttc ttttttttgt tttttttcac taggcataaa attttaaaaa
     1321 aaattcccaa actgacaaga agtttggggc ttaggcagct tcctctatct tctgctcttc
     1381 gtcaaccctg cgacgcatta ccgcggagat cgccttgagc aacttgattc tgttcagtag
     1441 tcccgttctg acctcagtca atctttccag gctgtgcttc agcatcaggt aatcatcggc
     1501 gaactctggc ttagccgtca gaatttcagg atcttctttg acacatccct cccaatggcg
     1561 ccaggtcggc attttcccct gaatctcaac atcaatccag aacaactctt tcttcaagtc
     1621 tttcatattc cggccagatg acagttcggt cgccctatcc gccaggcttt ccgagctaac
     1681 ttcttggagc tcttgctcca gattagttgt attgatgttt ttcagctctg actgcacttc
     1741 atcttgaatt cggcacaaaa cctcgtctct gggaccttcc gtgggactac tcaccacccc
     1801 tcctaagtaa aaagaccaag gacgcctatc ggcaaattaa aagtagcaat aaagtctata
     1861 tttgtcaaat ttttaaaaaa aatcttccct tccccgtgat atccctttct atcccctaat
     1921 atccccttat ctccccttgt ctccccttat ctccccttat ctccccttat ctccccttat
     1981 ctccc
//
LOCUS       DASWHZ010000021         4384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3022132, whole genome shotgun sequence.
ACCESSION   DASWHZ010000021 DASWHZ010000000
VERSION     DASWHZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022132"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..703
                     /locus_tag="VGA49_00530"
     CDS             284..703
                     /locus_tag="VGA49_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HEX9664288.1"
                     /translation="MNEKITKDMIVGQIGRQYPQTLEVFYKYDIDDCCNVDANLELAA
                     QDRGVDIEELIKDLNQAIAKPLVKQFAGVITKEMTMGEIVKQYPQVAPIMLDFGLHCV
                     GCHVAFWETLEEGIASHGMTEDDLDALLKQLNEAIKK"
     gene            740..1246
                     /locus_tag="VGA49_00535"
     CDS             740..1246
                     /locus_tag="VGA49_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9664289.1"
                     /translation="MNSNKLISLNEDEEVYKVIRRYWLNYFFAIFVTFILITLPFILI
                     VPLFQRGRWGMGLFFIILIFGLLSGLRTFISWYYNVFIVTSQRVIDIDQRGFFNKVVW
                     DTELDRIHDITYSKKGIWQTIFNYGNVQILTSGSLAKIEIINIKQPQKIKEAIKSIRE
                     EAKDGDLR"
     gene            1230..1631
                     /locus_tag="VGA49_00540"
     CDS             1230..1631
                     /locus_tag="VGA49_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX9664290.1"
                     /translation="MAIYDKKRKKHFIYKIIFSKNFIIICFIVLGLSTIALGKEIVRK
                     YKVNNEISALEAEINNLARENGDLLSLVQYLKTENFVEREARLKIGYQRPGENVVIVT
                     GDSSRAAEQAVVEETEDGNFYKWFVYFFGPK"
     gene            complement(1635..1707)
                     /locus_tag="VGA49_00545"
     tRNA            complement(1635..1707)
                     /locus_tag="VGA49_00545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1672..1674),aa:Thr,seq:cgt)
     gene            complement(1762..2220)
                     /locus_tag="VGA49_00550"
     CDS             complement(1762..2220)
                     /locus_tag="VGA49_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664291.1"
                     /translation="MAVQKTNNKKDLPFGFLESQLNHYRQIETSYNWQAAILVLTSGL
                     IFILSLQNIFSLDQRGVIGFLVIAFFNLMALAFSAYALKFKNNYPLEFNKKKIKQTLS
                     KPEKMTDYYLTELNYLANISIKSKSALTGYSVNLLIVGLTVGLILFFFLP"
     gene            complement(2306..2380)
                     /locus_tag="VGA49_00555"
     tRNA            complement(2306..2380)
                     /locus_tag="VGA49_00555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2346..2348),aa:Gly,seq:ccc)
     gene            2452..3132
                     /gene="ftsE"
                     /locus_tag="VGA49_00560"
     CDS             2452..3132
                     /gene="ftsE"
                     /locus_tag="VGA49_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX9664292.1"
                     /translation="MIHLKNVYKSYNSGVTALSGVNLHIKPKDFVSVVGQSGMGKTTL
                     AKLMIAQERPNKGRVVIGGWDITDIKDYQIPTLRRQIGVVFQDLYLLSDKTVYENVAF
                     ALEACGSPTKKIKKIVPQVLQLVGLLDKKDRYPRQLSGGEKQKVVLARSVVNRPKILI
                     ADEPTGNLDAINTEEIINLLLKINKFGTTVVLITHDREIVNSLNKRVIALEQGKVISD
                     KKTGKYTL"
     gene            3146..4060
                     /locus_tag="VGA49_00565"
     CDS             3146..4060
                     /locus_tag="VGA49_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX9664293.1"
                     /translation="MPASLLRALKYAFQNFWRNIWLSAITISIVVLALISINFLIILN
                     VITKTGVTEIEDKVDISVYFRPEVSENIIFEIKSKLLTLKNVKEIIYISREEALASFK
                     SNHQSDPQILKSLDEIDDNPLGATLIVKAKSTSDYPPILKVLNDLKYDDYIWDKDFEE
                     NEKIIQRISTISSRAKSIGLTVSVIFMMIALVVVYNTIRINIYTQRQEIGIMKLVGAT
                     NWFVRAPYLIDGLFYSLIGMIVTIVLIYPALGFLQPYMTTFFENSNFSLLAYFNQNFA
                     VIFGLELLAMMFLNSVSGFVATSKYLKV"
     gene            4159..4229
                     /locus_tag="VGA49_00570"
     tRNA            4159..4229
                     /locus_tag="VGA49_00570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4191..4193,aa:Gln,seq:ttg)
BASE COUNT         1371 a          763 c          907 g         1343 t
ORIGIN      
        1 ctggactggt aatcccgacg ccgtggccgg taccgttgcc tggcacaatg aagattttgt
       61 ccaggggacg gaactgaccc aaactctgtt caaataatag agtttatccc tgtcggtcac
      121 gaccgacggg accagtatca tcaagtcttt agctttgagg agaaagctcc tgacttagca
      181 aaaaccctgt cagtcgcgac tgacggggtt ttgcgttgag ataaaatgtg gtattttagg
      241 gtatagggga taggttatag ggtataataa ttaatttaca aatatgaatg aaaaaattac
      301 taaggatatg attgttggac aaattggccg gcaatatccc caaactctgg aagtttttta
      361 taaatacgat attgacgact gctgtaatgt tgatgcaaat ttagaattag ccgcccagga
      421 caggggagtg gatattgaag aactaattaa agatctcaat caggccattg ctaaaccttt
      481 ggttaaacag tttgccggag ttatcaccaa agaaatgact atgggcgaga ttgttaaaca
      541 atatccccag gtggcgccaa ttatgctgga ttttggcctc cattgtgtgg gttgccatgt
      601 ggcgttttgg gagactttgg aagagggaat cgcttctcac ggaatgactg aagatgatct
      661 ggacgcgttg ttaaaacagc tgaacgaggc gataaaaaaa taagcaaaca gtcaaccgcc
      721 aacagtcaac tcaataacta tgaattctaa caaactcatc agccttaacg aggacgaaga
      781 agtttacaaa gtcatcaggc gttactggct gaactatttt ttcgcgattt ttgtcacttt
      841 cattctgatc actctgccat ttatactgat tgtgcccttg tttcagcggg gtcggtgggg
      901 gatggggctg ttttttataa tattgatatt cggcttatta tctggcttga gaacttttat
      961 ttcctggtat tacaatgttt ttatagtcac cagtcagcgg gtaattgaca ttgaccagcg
     1021 gggcttcttc aataaagttg tctgggacac tgaactggac agaatccatg acattactta
     1081 cagcaagaaa ggcatttggc agactatttt taattacggc aatgtccaaa ttttgacatc
     1141 gggctccctg gctaaaattg aaattataaa tattaaacag ccccaaaaaa tcaaagaagc
     1201 gattaaatcc atccgggaag aagctaaaga tggcgattta cgataaaaaa agaaaaaaac
     1261 attttatata taaaataatt ttttcaaaaa attttattat tatttgtttt attgttttag
     1321 gcctgtcaac gattgctttg gggaaagaaa ttgtcagaaa atacaaagtt aacaatgaaa
     1381 tcagcgccct ggaggctgaa attaataatc tggcgcgaga aaatggcgat ttattgtcac
     1441 ttgttcagta cttaaaaact gaaaatttcg tggaacggga ggcccggctt aaaattggct
     1501 atcagcggcc gggcgagaat gtggtgattg tcaccggcga cagttcgcgg gcggctgaac
     1561 aagctgtcgt ggaggaaacc gaagacggta atttttataa atggtttgtt tatttttttg
     1621 gaccaaaata aaaaagccac ctgtcggatt cgaaccgacg acccgcggtt tacgataccg
     1681 ccgctctacc agctgagcta aggtggcgct tacatgaaag cattaaaaca atgaatcaaa
     1741 attgttttaa tgctcttatt tttaaggcag gaaaaaaaac agaatcagac caacggttaa
     1801 gccgacaatt aacagattga ccgagtagcc ggttaaagcg cttttagatt taattgatat
     1861 attagctaaa tagtttaatt cagtcaaata gtagtcagtc attttttccg gttttgacag
     1921 ggtttgtttt attttctttt tattgaattc cagcgggtaa ttgtttttaa attttaaggc
     1981 gtaagcggag aaagccaaag ccattaaatt aaaaaaggcg attaccaaaa agccgatcac
     2041 tcctctctgg tccagggaga aaatattctg gaggctcaaa ataaaaatta aacctgatgt
     2101 caaaaccaat atcgcggcct gccaattata gctggtttca atttgccggt agtgatttaa
     2161 ttgactttct aaaaaaccaa aaggtaagtc ttttttattg tttgtttttt gtacagccat
     2221 tttatttttt attttacaaa gcgagcgagg agaatcgccc gatcatccga tcgggcgccc
     2281 ggtcgaatga ccgggcgaac aagtatggag cgacggacgg ggatcgaacc cgtgacctcc
     2341 tgcttgggaa gcaggcgttc taccgctgaa ctaccatcgc atgttttttt tattttattt
     2401 agtctatgtt ataattataa aatattttaa ctaatctgaa aagcccaaaa aatgatacat
     2461 ttaaaaaatg tctataaaag ctataattcc ggcgtgacgg ctttgtctgg cgtaaacctc
     2521 catattaaac ctaaagactt tgtttcggtt gtcggccaaa gcggcatggg caaaactact
     2581 ttggctaaac taatgattgc tcaggaacgt ccaaacaagg gccgggtggt tattggcggc
     2641 tgggatatta ctgacattaa agattatcag atccctactt tgaggcggca gataggcgtg
     2701 gtttttcagg atctgtattt gctgtccgat aaaacagttt acgaaaatgt cgcttttgcc
     2761 ctggaagcct gcggctcgcc gactaaaaaa attaaaaaaa tagttcctca agttctccag
     2821 ctggtcggtt tacttgataa aaaagaccgt taccccagac agctgtcagg cggcgaaaaa
     2881 caaaaagttg ttctggccag atcggtcgtt aacagaccca aaatattaat tgccgatgaa
     2941 ccaactggta atctggacgc gattaatacc gaagagatta ttaatttgct tctaaaaatt
     3001 aataaatttg ggacaactgt tgttttaatt actcatgacc gggaaattgt taattcgctg
     3061 aacaaacggg ttatcgccct ggaacaggga aaagtaatca gtgataaaaa aacaggaaaa
     3121 tacacgcttt aacccccctt aatttatgcc ggcatctctc ttaagagctt taaaatacgc
     3181 ttttcaaaac ttttggcgca atatctggct gtcggccatc actatttcaa ttgttgtttt
     3241 ggcgcttatt tcaattaatt ttttaattat tctaaatgtt atcaccaaaa ctggcgtcac
     3301 tgaaattgaa gataaggttg acattagtgt ttatttcagg ccggaagttt ctgaaaatat
     3361 tatatttgaa attaaatcaa agttactgac tctaaaaaat gtcaaagaaa taatttatat
     3421 ttccagagaa gaagctttgg cgtcttttaa gtccaatcat cagtcagatc ctcaaatttt
     3481 aaaatcgctt gatgaaattg acgataatcc cctgggggcg accctgatag ttaaggctaa
     3541 atcaaccagc gactatccgc cgattttaaa agttttaaat gacctaaaat atgatgacta
     3601 tatctgggat aaagattttg aggaaaatga aaaaattatt caaaggatta gcaccatcag
     3661 cagccgggcc aaatcaatcg gattgaccgt ttcagttatt tttatgatga ttgcccttgt
     3721 cgtggtttat aatactatca gaattaatat ctacacccag cggcaggaaa ttggcattat
     3781 gaagctggtt ggcgccacta attggtttgt tcgggcgcct tatctgattg acggcctgtt
     3841 ttatagtcta atcggaatga ttgttactat cgtcttaatt tatccggcat tggggttttt
     3901 acagccgtac atgacgactt tttttgaaaa ttcaaacttc agcttactgg cttattttaa
     3961 tcaaaatttc gcagttatat ttggactgga attgctggca atgatgtttt taaattctgt
     4021 cagcggtttt gtggcgacat ctaagtatct gaaggtctaa atacaaaatt caaaatgtaa
     4081 agataaaaag tttgtaattt ataattttgt atttattatt tatttaggat ttcgaattta
     4141 gaatttatga tttattattc ggggatcgtc taatggcagg acaccaggtt ttggtcctgg
     4201 gtatctaggt tcgaatccta gtccccgaga atgtaaaaat taaaacaaat ttatgaaaat
     4261 tatttttttg gctagtctta gcaaaattag agctcaaaaa tttaaatatt gtagtttttc
     4321 cacaaaaaca ttagaaaaat aagacttttt gtgatattat taaataataa acattaaaat
     4381 tgtt
//
LOCUS       DASWHZ010000022         6278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1540954, whole genome shotgun sequence.
ACCESSION   DASWHZ010000022 DASWHZ010000000
VERSION     DASWHZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540954"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VGA49_00575"
     CDS             complement(<1..929)
                     /locus_tag="VGA49_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX9664294.1"
                     /translation="MQFQIFQDNQKGQWNDFIKANALDGGLLQSWQWGDFKKSLGHQV
                     WRVGLVDQGQILIASQLIRHKKLAFNFLYSPRGLVISKEVMRQMKIKEVLGQFLIEIK
                     DLAKKQKAIFLRVDPAWPRTSPRLLPDPGRGKSGDVLPEKFLTEAGFKSIENLIQPIH
                     TLILDLTKNSEELLAQTHHKTRYNIGLAERKGLTVKADHNQLDVFYNLLSKTKERQAI
                     GIHSKKYYQKMTEVLAPEKMLNIFTAEFQGKNLAAILVAYFNQTAYYLHGGSDPAFRQ
                     LMAPHLLQWTAIQEAQKNGYKFYDFWGVAPDVV"
     gene            complement(1101..3500)
                     /gene="leuS"
                     /locus_tag="VGA49_00580"
     CDS             complement(1101..3500)
                     /gene="leuS"
                     /locus_tag="VGA49_00580"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9664295.1"
                     /translation="MNYDPQKIEPKWQKKWEKLGLNQARDFGKKPKYYCLDMFPYPSA
                     AGLHVGHPEGYTATDILSRYKRMNGFNVLHPMGWDAFGLPAENYAIKMKVNPAKITKE
                     NIKNFTRQIKSLGFSYDWNREINTSDPDYYKWTQWLFLLLYKNNLAYKKKAPVNWCPH
                     CQTVLANEQVIDGKCERCKNEVVQKELEQWFFKVTKYADELLNGLDELDWPEPLKQIQ
                     RNWIGKSAGAEIDFKVKGSRQAIKVFTTRPDTLFGATYLVLAPENSLVQELVAEDRKQ
                     EAEKYIKAAQKKSHLERTDLAKDKTGVFIGAYAVNPATNQEIPVWISDYVMMDYGTGA
                     IMAVPAHDQRDFEFAKKFKLPIFQVIRPDKEVIKGENQAGALKEAYTGDGQLINSGKF
                     DGLKAEQAKKEITKAVQGRKKTIYKIRDWLISRQRYWGAPIPIIYCDQCGQVPVPEKN
                     LPVILPDDVDFKPTGESPLVKSKKFHNVKCPQCGGAARRESDTMDTFVCSAWYYFRYL
                     DPRNSQQFCGQKKIKYWLPVDLYIGGAEHAAGHLIYSRFITKVLRDLGYVGFEEPFLK
                     LVNQGLILAEDGRKMSKSLGNVINPDEVVARYGADTMRMYEMFMGPLEDTKPWNAQGM
                     VGVRRFLDKVWSLRQTDNYVENKEMVVVTHKTINKVTEDIKNFKFNTAVSALMIWVNE
                     MTAKDHLLVLDYQNFLKILAPFAPHIAEELWSALGKKESIFQESWPKHDDRLTVDQTV
                     TLIVQINGKVRDKIEAKADLTEEEAKNLVYGSGRIKTYIEGKKIKKIIYIQGRLINIV
                     V"
     gene            3862..5175
                     /locus_tag="VGA49_00585"
     CDS             3862..5175
                     /locus_tag="VGA49_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9664296.1"
                     /translation="MSRPIKKIFVKFLVFLIRSINNLLKKLMPFFIGKIAKPFFWLFH
                     LIYKNYHFIKKLLAKIYQPAKNRFFYPFFGKSVIHIIIIIIIFGVTFSNLQASAVSTI
                     GQKSLLYSMVADENLQLIEETADLTREKKIAHLGPSVGVISVPLALAADEAEKENIQQ
                     LTTTTQGASALVKPNITSPLTGNQNRLRDETETYLVQENDTISSIAKDFGISADTILW
                     ENNLNARGFIKPGQKLTILPTSGLSHKVKSGDTIGSLAKKYSVEQKDILAFNEILDAS
                     EIKIGQTLIIPDGTPVTAPRPAPKTQLANIRDIFMPAPARPSAARLQWPTSGRVITQY
                     YTWRHSGLDIDGHYDSPIYAAEGGTVEFTGWASGYGLSVVVNHGNGMKTRYAHASKIF
                     VNPGQQVSRGQTLAMVGTTGWSTGTHLHFEVMINGVRQNPLSYVR"
     gene            complement(5494..>6278)
                     /locus_tag="VGA49_00590"
     CDS             complement(5494..>6278)
                     /locus_tag="VGA49_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9664297.1"
                     /translation="CPTLRCADLSGLDLAGIDLRKADLYKAKLAGANLAGADLRGSDL
                     GWAAFQHADLTGADLSGLSIQGAKFEKACLARAILRGVYFYGDFWEADFREADLRGAV
                     LQSCEFHQANFSRANLTRAVLESARMSQTNFSGADLTKVVFNRMQGGASFSGAKLEST
                     SFIFLFQHFDGDFSQADLDRVCFRRADLKRANFSGASIVRTDFFETNLQGADFSNSQL
                     ESVDFEGVDLEGASFSGARITGVNFERARNVPAAVELAAASD"
BASE COUNT         1774 a         1481 c         1253 g         1770 t
ORIGIN      
        1 ttaaccacat ccggcgccac tccccagaag tcgtaaaatt tatagccatt cttttgagcc
       61 tcctgaatcg ctgtccattg aagtagatga ggcgccatca gctggcgaaa agccggatcg
      121 gagccgccat gcagataata agccgtctgg ttgaaataag cgaccaaaat agccgccaaa
      181 ttttttccct gaaattcagc ggtgaaaata ttaagcatct tttccggcgc caaaacttca
      241 gtcatttttt gataatattt tttggaatga atgccgatcg cctggcgttc tttggttttg
      301 gataaaagat tataaaaaac atcaagctga ttatgatccg ctttaactgt cagtcctttt
      361 ctttccgcca gcccgatatt atacctggtt ttatgatgcg tttgagccaa cagctcttca
      421 gagtttttgg ttaagtctaa aattaaagta tgaattggct gaattaaatt ttctatactc
      481 ttaaaaccag cctcggttaa aaacttttcc ggtaaaacat cgccagactt gccccgtccc
      541 ggatccggca gcagccgggg actggtgcgg ggccaggctg gatcaactct taaaaaaata
      601 gccttctgtt ttttagccaa atctttaatt tcaatcaaaa actgccctaa aacttctttg
      661 attttcatct ggcgcatgac ttcttttgaa ataactaacc ctctggggct gtataaaaaa
      721 ttaaaagcca gttttttatg ccggatcagc tggctggcga ttaaaatttg tccctgatca
      781 accagtccca cccgccaaac ctggtggccc aggctttttt taaaatcgcc ccattgccag
      841 gactgcagca aaccaccatc taaggcgttg gctttgatga agtcgttcca ttgtcctttt
      901 tgattatcct gaaaaatttg aaattgcata taaataaagg caataggcac aaggcacagg
      961 gcacaagtgt tggaattatt ttattagatg gggatcaggc aaataagaat aggcaatgga
     1021 caaaaagcac aagtgctgga attgtttttt atactattgc ctcatccctt ttgcctcgcc
     1081 tactgccttg tgccttgtgc ctaaacaaca atattaatca acctcccctg aatgtaaata
     1141 atcttcttaa tcttttttcc ttctatatac gtcttaattc ggccactgcc gtaaactaaa
     1201 ttcttagctt cctcttcagt caaatcagcc ttagcctcaa ttttatccct gactttgccg
     1261 ttaatctgaa caatcaaagt tacagtctgg tcaacggtta atctatcgtc atgctttggc
     1321 cagctttcct gaaagatgga ttcttttttg cccagggccg accaaagctc ttcagcgata
     1381 tgcggcgcga acggcgccag aatttttaaa aagttttgat aatccaaaac cagcaagtga
     1441 tctttggcgg tcatttcatt aacccagatc attaaggcgc tgacagcggt gttaaattta
     1501 aaattcttaa tgtcttcggt cactttgtta attgtcttat gagtgacgac caccatctct
     1561 ttattttcaa cgtaattatc tgtctgacgc aggctccaga ctttatccaa aaatcgtctg
     1621 actccaacca tcccctgagc attccatggt ttggtgtctt ccagcggccc catgaacatt
     1681 tcatacattc tcattgtgtc cgccccgtac cgggcaacca cttcatccgg attaatgaca
     1741 ttacccagag acttggacat tttccggccg tcttccgcca ggatcagtcc ctggttaacc
     1801 agttttaaaa atggctcttc aaaaccaaca taacccagat cgcgcaaaac cttagttata
     1861 aacctggaat aaatcaaatg cccggcggcg tgctcggccc cgccaatata taaatcaacc
     1921 ggcagccaat atttaatttt tttctggccg caaaattgct gactatttcg gggatccagg
     1981 taacggaagt aataccaggc cgagcagaca aaagtgtcca ttgtgtctga ttcccgcctg
     2041 gccgccccgc cgcattgagg acattttaca ttatgaaatt ttttggattt aaccagcggc
     2101 gattcgccgg tcggcttaaa atcaacatca tccggcagaa taaccggcag gtttttttca
     2161 gggacgggaa cctgaccgca ctggtcgcaa taaataatcg gaatcggcgc cccccagtat
     2221 ctttgccggg aaatcaacca gtcccgaatt ttataaattg ttttttttct gccctgaacc
     2281 gctttggtaa tttccttttt ggcctgttca gcttttaagc cgtcaaattt tcctgaatta
     2341 attaattggc catcgccagt ataggcttct tttaaagccc cggcctgatt ttcaccttta
     2401 ataacttcct tgtcaggtcg aatgacctga aatattggca atttaaactt tttggcgaat
     2461 tcaaaatccc gctggtcatg agccggcacg gccataatcg ctcccgttcc gtaatccatc
     2521 atgacgtaat ctgaaatcca aaccggaatc tcctgattag tggccgggtt aacggcgtaa
     2581 gcgccgataa aaacgccggt tttatcttta gctaaatcag ttctttccaa atgcgatttc
     2641 ttttgggcgg ctttaatata tttttctgct tcttgtttcc tgtcttccgc gaccagctcc
     2701 tgaaccaaag aattttcagg agccaaaacc agataagtcg ccccaaacaa agtatcaggc
     2761 cgggtggtaa aaactttaat tgcctggcgg ctgcctttga ctttaaaatc tatttccgct
     2821 cccgcggatt tgccaatcca gtttctttga atctgtttga gaggttcggg ccagtcaagc
     2881 tcgtctaaac cgttcaataa ttcatcagcg tatttcgtta ctttaaaaaa ccactgctcc
     2941 agctcttttt ggacgacttc atttttacag cgctcgcact tgccgtcaat cacctgctca
     3001 ttggccagca cagtctggca atgggggcac cagttgaccg gagccttttt tttataagct
     3061 aaattatttt tgtataataa taaaaataac cattgagtcc atttataata atccggatcg
     3121 gaagtattaa tttcacgatt ccagtcatac gaaaaaccaa gtgacttaat ctgccgggta
     3181 aaatttttaa tattttcttt ggtgatttta gccgggttaa ccttcatctt gatggcgtaa
     3241 ttttcagccg gtaaaccaaa agcatcccag cccatgggat gaagaacatt aaaaccattc
     3301 attcgtttat agcgggaaag aatgtctgtc gccgtgtagc cttcagggtg gccgacatgg
     3361 aggccggcgg ctgacggata gggaaacata tccaggcaat aatatttggg ctttttacca
     3421 aaatcccggg cctgattcag acccagtttt tcccattttt tttgccattt tggctctatt
     3481 ttttgaggat cataattaat catagtaatt aattaaatgt agctgatatt ggctaaataa
     3541 gcaatttggc cttgacaaaa ttattataat atgctatatt tatacgatat cagctgattt
     3601 attaaaaatc gccacaggca aggcagcccg gtccaaacgg gctgcttatc aattgaattg
     3661 acactgacga ctgaaatgct atatttatgt taaatgttat tatgttatcc gaattcacaa
     3721 attttatccg aattgacaaa taacgaatat aagttggtgt tatcctaatt gacaaattat
     3781 atccgaatta acaaataaca aatttgttgt attagttatt cgtagattag gataaaattg
     3841 gttaattagg ataacgttac tatgtcccga ccaatcaaaa aaatctttgt aaaatttctt
     3901 gtttttctga ttaggagcat taataatctt ttgaaaaaat taatgccttt ttttattggc
     3961 aaaatcgcta aaccattttt ctggctgttt catttaattt acaaaaatta tcattttata
     4021 aaaaaactgc tggctaaaat ttaccagccg gccaaaaaca gattctttta tccttttttt
     4081 ggcaaatcag ttattcatat tatcattata attattattt ttggagtaac cttcagcaac
     4141 cttcaggcct cggctgtcag cactatcggc cagaaaagtc ttttatactc aatggtggcc
     4201 gatgaaaatc tccaactgat cgaagaaacc gctgacctta cccgggagaa aaaaatcgcc
     4261 cacctggggc cgtcggtcgg tgttatcagc gttcccctgg cgctggcggc tgatgaagct
     4321 gaaaaagaaa atatccagca gctgacaacc accacccagg gcgccagcgc tctggttaaa
     4381 ccaaacatca cttcaccgct gaccggcaat caaaaccggc tgcgggatga aacggaaaca
     4441 tatcttgtcc aggaaaacga tactatcagc agtatcgcca aggattttgg catttcagct
     4501 gacaccattc tctgggaaaa taatttaaac gcccgcggct ttattaaacc tggtcaaaaa
     4561 ctaactattc tgccaaccag cggcttgtct cataaagtta aaagcggcga cacgattggc
     4621 agcctggcta aaaaatacag cgttgagcag aaagacattt tggcctttaa tgaaattctg
     4681 gacgcttctg aaattaaaat cggccaaact ctaattattc cggacggcac gccagtcacc
     4741 gctccccggc cggcgccgaa gacgcaactg gccaacatcc gggatatttt catgccggcg
     4801 ccggcccgac ccagcgccgc ccgtctccag tggccgactt ccggccgggt gattacccag
     4861 tattacacct ggcggcacag cgggctggac attgacggcc attatgattc gccgatttac
     4921 gccgctgaag gcggcacggt tgaatttacc ggctgggcca gcggctacgg cttgagcgtc
     4981 gtcgttaacc acggcaacgg aatgaaaacc agatacgccc acgccagcaa aatatttgtt
     5041 aatcccggcc agcaggtcag ccggggccag actctggcca tggtcggcac caccggctgg
     5101 tcaaccggaa ctcatcttca ttttgaagta atgattaatg gggtcaggca aaatccgttg
     5161 tcgtatgtaa gatagggggg gataagggtt gatgagggtt aatgaaggtt gatgagggga
     5221 ataagggaat gagaaggaat aagaataaac tattaaattt caaattacaa aattcaaatg
     5281 tcaattcaaa tccaaaatct aaaatccaaa aattttatta tttgacattt aaactttgga
     5341 tttcaattga tatttgggct ttgatatttg gatttaatag aaagggaagc aaaagagatc
     5401 aaaagaaatt aaaaatccgg ttaattaatc cagccggttt tttgattata aaaaaacgga
     5461 cctgtgacaa agtccgttat tattcctggt aattcaatcg ctggccgcgg ccaattccac
     5521 tgccgcagga acatttcgcg ctctttcaaa attgaccccg gtaatcctgg ctcccgagaa
     5581 actggctccc tcaaggtcaa ccccttcaaa atctacactt tcaagctggc tgttagagaa
     5641 atcagcccct tggagattgg tctcaaaaaa gtcagtcctg acaatactgg ctccggaaaa
     5701 attagctctt ttcaggtcag cccgacgaaa acaaacccgg tccagatcag cctgactaaa
     5761 atcgccgtcg aaatgctgaa aaagaaaaat gaagctggtc gactctaatt tggctccgct
     5821 gaagctggcg cctccctgca tccgattgaa aacaaccttg gtcaaatccg ccccagaaaa
     5881 attggtttgg gacattctgg cgctttccaa aacggctctg gtaagattcg ctctggaaaa
     5941 attggcctga tgaaactcac acgactgcag aacagcccct cgcaaatcag cttcacgaaa
     6001 atcagcctcc caaaagtccc cataaaaata gacccctcgt aggatggccc gggccagaca
     6061 agctttctca aacttagccc cctgaatact aaggcctgac agatcagctc cggtcagatc
     6121 ggcatgttga aaagcggccc agccaaggtc agagcctcgc aagtcggctc cggccaggtt
     6181 cgccccagcc agcttggctt tatacagatc agcttttctg agatcaattc cagccagatc
     6241 taatccggaa agatccgcac atctcaaggt agggcaga
//
LOCUS       DASWHZ010000023         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1544246, whole genome shotgun sequence.
ACCESSION   DASWHZ010000023 DASWHZ010000000
VERSION     DASWHZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544246"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGA49_00595"
     CDS             <1..229
                     /locus_tag="VGA49_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9664298.1"
                     /translation="IQESTKEILKNVIKLNKHLGAYQDYLKKLGGHLGTTVNIYNLAN
                     KEFSKIDKDVARISGEETSFEPELLDKPSVE"
     gene            318..554
                     /locus_tag="VGA49_00600"
     CDS             318..554
                     /locus_tag="VGA49_00600"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9664299.1"
                     /translation="MAFKQSLLGALSLAFELGYTIAVPLVVLALTGRLLDSRYQTSPW
                     FLLAGILLSIVVSSWLVYKKTLKIFADIEQEAKK"
     gene            557..1297
                     /gene="atpB"
                     /locus_tag="VGA49_00605"
     CDS             557..1297
                     /gene="atpB"
                     /locus_tag="VGA49_00605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9664300.1"
                     /translation="MNISLAAEPIFHIGTLPITNTLIVSWLLVILLVAFSLLVKNKFR
                     AVPGKLQNFLEFTIGGAFDFFDSIVGDRKQTRKFFPIVATIFIFVLLSNFVELWPGLG
                     SIGIYEVHNGEEVLIPFIRSASADLNFTLAIAIVSVISTQILGIAAIGFFKYSSKFFN
                     FRGPIQFFVGILELVSEFAKIISFSFRLFGNIFAGEVLLLVVGFLVPYIVPLPFLFLE
                     IFVGLVQALVFSMLTLVFLKMAVFEHAH"
     gene            1403..1630
                     /gene="atpE"
                     /locus_tag="VGA49_00610"
     CDS             1403..1630
                     /gene="atpE"
                     /locus_tag="VGA49_00610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX9664301.1"
                     /translation="MDLDMIKSIMAGLTIAVGAIFPALAIGKMGAKAVESIGRNPEAA
                     AKIQTVMILAIAFAEAIAIYALVIALIIKFV"
     gene            1783..2298
                     /gene="atpF"
                     /locus_tag="VGA49_00615"
     CDS             1783..2298
                     /gene="atpF"
                     /locus_tag="VGA49_00615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX9664302.1"
                     /translation="MELIQKLGIDWRLLIGQIINFLILLFILYKFVYNPLLDILDKRQ
                     SKVEKSLVDAKKIEENLREAEIRKEKEILKAKKEAQTIIEKAKAAAENYRKETEQKTK
                     EKIKTLIAESKEVIKDEKDKMFQSLRGEIADLVILTTKGMIGKELDQKKDKEIINIAI
                     KDLSREIYEKD"
     gene            2285..2686
                     /gene="atpH"
                     /locus_tag="VGA49_00620"
     CDS             2285..2686
                     /gene="atpH"
                     /locus_tag="VGA49_00620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX9664303.1"
                     /translation="MKKISPGQYALTLDLITAEADKKDIPRLIDGLIGLMTRNNDLGL
                     ADQVINQYQKLVKDQQGEIDIEVVSAKKIEPDQKKDLEQKLLTKNIKQININNVIDKN
                     ILGGLVVKYKDFILDASLVSQLNNLQQSLKQ"
     gene            2691..>3902
                     /gene="atpA"
                     /locus_tag="VGA49_00625"
     CDS             2691..>3902
                     /gene="atpA"
                     /locus_tag="VGA49_00625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9664304.1"
                     /translation="MAETKDYIIETLVDRIKSYKARTEVKEVGQVVEIKDGIATLTGL
                     ANVRVAEMLDFGNDVFGVVLNLEQDLVGAMILGDYLKIKEGDQVSRTDKILEVGVDDK
                     LVGRVINPLGQVLDGKAVVKFKKFYPMEKIAPGVITRQSVNQPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQKGEDVICIYVAVGQKESKVARLVARLEEAGAI
                     EHTIVVSASASDPASLIYIAPFAGCAIGEYFMDQGKDVLVIYDDLSKHAVAYREISLV
                     LRRPPGREAYPGDIFYLHSRLLERAAKLNKDYGGGSLTALPIIETQAGDISAYIPTNV
                     ISITDGQIYLESDLFYQGIRPALNVGLSVSRVGSAAQIKAMKKVAGKLRLDIAQYREL
                     AAFAQFAAELDE"
BASE COUNT         1138 a          711 c          904 g         1149 t
ORIGIN      
        1 aattcaggaa tcaaccaaag agattttaaa aaatgtaatt aaattgaata aacatctggg
       61 ggcttatcag gactatttaa agaagctggg cggtcatttg ggaacgactg ttaatatata
      121 taatttagcc aataaagaat tctccaaaat tgacaaagat gtcgcccgaa tcagcggcga
      181 ggagacgtca tttgagccgg aactgctgga taagccgtca gttgaataat atttgactaa
      241 aattagccaa atattatttt agtttattga ctaggggacg attgttctct ataatgaatt
      301 acctaagttt agcgattatg gcctttaagc aatcattatt aggagcgtta agcctggctt
      361 ttgagctggg gtataccatt gccgtgccat tagtcgtttt ggcgctgact ggacgattgc
      421 ttgacagccg ttatcagaca tcgccctggt ttttactggc aggtatttta ttgtcaattg
      481 tggtttcatc ctggctggtt tataaaaaga ctttaaaaat atttgctgat atagagcaag
      541 aagccaaaaa ataactatga acatttctct ggccgctgaa ccaatatttc acatcggcac
      601 tttaccaata accaacaccc tgattgtttc ctggttgttg gtgattttgc tggtcgcgtt
      661 ttccttgttg gttaaaaata aattcagagc tgttcctggt aaattgcaga attttttgga
      721 attcacgatc ggcggggcct ttgatttttt tgattcaatt gttggagaca gaaaacagac
      781 cagaaaattt tttccgattg tggctactat ttttattttt gttttattat caaattttgt
      841 tgagctttgg cccggcctgg ggtcaatcgg tatttatgaa gttcataatg gtgaggaagt
      901 tttaatcccc tttatccggt cggcctcggc tgatctgaat tttactttag cgattgccat
      961 tgtatcggtt atttcaactc aaattttggg aatcgccgcc atcggttttt ttaaatattc
     1021 atctaagttt tttaatttta gagggccgat tcagtttttt gtcggcattc tggagctggt
     1081 ctcagagttc gccaaaatta tttcattttc tttccggctg tttggcaata ttttcgccgg
     1141 cgaggtttta ctgctggtcg tgggatttct ggttccttat atcgttcctc tgcctttctt
     1201 atttttagaa atattcgttg gcctggtcca ggccctggtt ttttcaatgc tgactttggt
     1261 ttttttaaag atggccgtgt tcgagcacgc ccactaacaa ctaactacta acgaccatta
     1321 ttcccgccga ctataaaaaa caatacttaa aaacccagga cgggtagtcg ccagcgggat
     1381 gattgtcaac aggagaaata atatggatct ggatatgatt aaaagcatca tggccgggtt
     1441 aaccatcgcg gttggcgcga tttttccggc tttagctatt ggcaaaatgg gggccaaggc
     1501 ggttgaatca attggccgca atccggaagc cgccgccaaa attcaaactg tcatgatttt
     1561 ggcgattgct ttcgccgaag ccatcgccat ttacgccctg gttatcgctc tgattattaa
     1621 gtttgtctaa ttttagttcg gtttctcgtt ccgtattaaa tttacggagc gagttctgag
     1681 tttaaatcag ccaatagtca acagccaaca gccagcaggg caagattaaa aaataagagc
     1741 tccccgttga cggttgactg ctgactgttt aactaaaaaa ttatggaatt aattcaaaaa
     1801 ctaggaattg actggcggct gttaattggt caaattatca attttttgat attgctgttt
     1861 attttatata aatttgttta caatcctttg ctggatattt tggacaaaag acagagcaaa
     1921 gttgaaaaaa gtctggttga cgccaaaaaa atagaagaaa atctgcgtga agctgaaatc
     1981 cgcaaggaaa aagaaatact caaagccaaa aaagaagccc agacgattat tgaaaaagcc
     2041 aaagcggccg ctgaaaatta ccggaaagaa actgaacaga aaaccaaaga aaaaattaaa
     2101 accctgatag ctgaatccaa agaagtaatt aaagatgaaa aagataaaat gtttcagagt
     2161 cttcgggggg agatagccga tttggttatt ttgacgacca aagggatgat cggcaaagag
     2221 cttgatcaga aaaaagataa agaaattatt aatatagcga ttaaggatct aagcagagag
     2281 atttatgaaa aagattagtc ccggacaata cgctttgacg ctggacttga tcacggccga
     2341 agctgacaaa aaagatattc cccgtctgat agacggtttg atcgggctga tgaccagaaa
     2401 taatgatttg ggactggctg accaggtgat taatcagtat cagaaactgg ttaaggatca
     2461 gcagggagaa attgacattg aagttgtttc ggccaaaaaa attgagcctg atcaaaaaaa
     2521 agatttggag caaaaactgc tgactaaaaa tattaagcag attaacatta ataatgttat
     2581 tgataaaaat attttgggtg gtctggtggt taaatacaaa gattttattc tggacgccag
     2641 cctggtcagt cagcttaata atttacaaca atcattaaag caataaattt atggcggaaa
     2701 caaaagatta tattatagaa actttagttg accggattaa aagttataaa gccaggacgg
     2761 aagttaagga agtcggccag gtcgttgaaa taaaagacgg tattgccact ctgaccgggc
     2821 tggctaacgt ccgggtggcc gaaatgcttg attttggcaa tgatgttttt ggagttgttt
     2881 taaatctgga gcaagatctg gttggcgcca tgattttggg agattattta aaaattaaag
     2941 aaggtgatca ggtcagccgg acagataaaa ttttggaagt cggcgttgac gacaagctgg
     3001 ttggccgggt gattaatccg ttgggtcagg ttttggacgg caaggctgtt gttaaattta
     3061 aaaaattcta cccaatggaa aagatcgctc ccggggtgat taccaggcag tcagttaacc
     3121 agcctttgca gaccgggatt aaagcgattg actcaatgat tccaattggc cggggtcagc
     3181 gggagctgat tattggcgac cggcagaccg gtaaaaccgc tctggcgatt gacgccatta
     3241 ttaatcaaaa gggcgaggac gtaatttgta tttacgtggc tgttggccag aaagaatcaa
     3301 aagtggccag gctggtggcc aggctggaag aagccggcgc cattgaacac accattgttg
     3361 tttccgcttc agcttctgat ccggcctcat taatttatat tgctcccttt gccggctgcg
     3421 ccataggtga atattttatg gatcagggaa aagatgttct ggtgatttat gatgatttat
     3481 ccaaacacgc cgtggcttac cgggaaattt ccctggtgct gcgccggcct cccggacggg
     3541 aagcttatcc cggagatatt ttttatctgc attccagatt gctggaaaga gccgctaaat
     3601 taaacaaaga ttatggcggc ggttcattga cggccctgcc aattattgaa actcaggcgg
     3661 gcgatatttc cgcttatatt ccaaccaacg tcatttcaat taccgatggt cagatttatc
     3721 tggaaagcga tctattttat cagggaattc ggccggcctt aaatgttggt ttgtctgttt
     3781 cccgggtcgg ctcagccgct cagattaaag ccatgaaaaa agtggccggc aaacttcggc
     3841 tggatatcgc tcaatacaga gaactggcgg cttttgccca gttcgccgcg gagctggatg
     3901 aa
//
LOCUS       DASWHZ010000024         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1175793, whole genome shotgun sequence.
ACCESSION   DASWHZ010000024 DASWHZ010000000
VERSION     DASWHZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175793"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1181)
                     /locus_tag="VGA49_00630"
     CDS             complement(15..1181)
                     /locus_tag="VGA49_00630"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9664305.1"
                     /translation="MKLKTLQNFKVKNKKVLLRADLNVAYDPVRKRILNDYDLIKILP
                     TIKYLLKNKAQIILCSHFGSQENYSLAPVAVKLSGLLKVKVKLAPDSVGSKVEKLISQ
                     TAPGEIILLENLRFNKGEKGNDQQFAKALAGLAGVFVNDAFGVMHRRHASIVGVPKYL
                     ESVAGPLVEQEVKALSKILTKPKRPLVVIVGGVKISSKIATINNLAGMADQVLLGGSL
                     ANTFLAAKGVKIGSSADGSEIKLATKLLKKHQSKIILPVDVVVAVKKGRKFIQPENKP
                     AQIDGFKKNETVLDIGQQTTKDYCRLISSAQMIFWGGPLGLIEDSRFIKGTKTVVKCI
                     AKAKAFTVAAGGDTVAAIDQLGQLSKFDFISTGGGATLEFLAGKKLPGLQPLST"
     gene            complement(1356..1462)
                     /gene="rrf"
                     /locus_tag="VGA49_00635"
     rRNA            complement(1356..1462)
                     /gene="rrf"
                     /locus_tag="VGA49_00635"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          427 a          372 c          308 g          449 t
ORIGIN      
        1 aaaatatcca cagactatgt ggataaaggt tgcagcccgg ggagcttttt cccggctaaa
       61 aattcaaggg tcgccccgcc gccggtggaa ataaaatcaa attttgacaa ctggccaagc
      121 tgatctatgg cggcgacagt atcgccgcct gcggccactg taaaagcttt tgctttagcg
      181 atacatttaa cgaccgtctt ggtgcctttg ataaaccggg aatcttcaat taaacctaaa
      241 ggaccgcccc aaaaaatcat ttgggctgaa ctgatcaacc ggcaataatc cttggttgtc
      301 tgttgaccga tatctaaaac ggtttcattt tttttaaaac cgtcaatttg agccggctta
      361 ttttcaggct gaataaactt ccggcctttt ttaacggcaa caacgacatc aaccggtaaa
      421 ataatttttg actggtgttt ttttaaaagt ttcgtggcca gtttaatttc agagccatcg
      481 gccgaacttc ctattttaac gcctttagcc gccagaaaag tgttggccaa agacccgccc
      541 agcaaaacct gatcagccat tccggccaga ttattaatag tggcgatctt gctggaaatt
      601 ttgacaccgc caacaatcac caccagcggc cttttgggct tagtcaaaat ttttgataac
      661 gctttaactt cctgttcaac cagcgggccg gcgacgctct ctaaatattt tggaacgcca
      721 acgattgaag cgtgccgtcg gtgcatcacc ccaaaagcgt cattcacaaa aacaccggcc
      781 agtccggcca aagctttggc gaattgctga tcattgcctt tttcaccttt attaaacctt
      841 aaattctcca gcaaaataat ttctccgggg gcagtctggc tgatgagctt ttccacttta
      901 gaaccaactg aatcaggggc taacttaact tttactttta ataatccgga taatttgacg
      961 gccactggcg ccaaagaata attttcctga ctcccaaaat gggaacacaa aattatttgg
     1021 gctttgtttt ttaaaagata cttaatcgtc ggcaaaattt tgatcagatc gtagtcattc
     1081 aatattcttt ttctgaccgg atcataagca acattcaaat cagcccgcag taaaactttt
     1141 ttatttttaa ccttaaagtt ttgcaatgtt ttcagcttca tattaattaa tcgtagcaca
     1201 aattttgcgt aatcaccaaa ccgcaccgtc ccgccttggc gggacggtat gcggttgttg
     1261 atcccgccgt tggcgggatt tattttttgc acccattcat ccctgcggca agccgcaggg
     1321 tattctgggc aaaaaacaaa aaaagagtta caaaactctg gcgacgtcct actctcgcct
     1381 tgcgactacc atcggggctg agaggcttaa ctactgtgtt cgggatggga acaggtgtga
     1441 ccctctcgct agaatcacca gaattttata actctttttt aaagtaaaat tatttcaaca
     1501 tcttaatggt tacagacctc gacttattag tacggctcgg ctcaatccct tgcgga
//
LOCUS       DASWHZ010000025         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_69720, whole genome shotgun sequence.
ACCESSION   DASWHZ010000025 DASWHZ010000000
VERSION     DASWHZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69720"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..617)
                     /gene="nusB"
                     /locus_tag="VGA49_00640"
     CDS             complement(99..617)
                     /gene="nusB"
                     /locus_tag="VGA49_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX9664306.1"
                     /translation="MSNRHLARTIAMQTLYEWDFNGRPANQLNAYLKHNMSEFAPDFD
                     DGGFTKHLLEGVTAKIKEIDQSITKYAPEWPLDQITIVDRNILRIGIYELIFDNSIPD
                     KVAINEAIELAKTFGGESSGKFVNGVLGAVYKDLLDKGIKQKDEETNKTKEQKDKGTE
                     QLKNGKTERLSN"
     gene            complement(621..815)
                     /gene="rpmF"
                     /locus_tag="VGA49_00645"
     CDS             complement(621..815)
                     /gene="rpmF"
                     /locus_tag="VGA49_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9664307.1"
                     /translation="MGVPTQRRTKSSKGKRASHFALKKIKLFKCPQCQKPVQPHHACK
                     YCGYYKGREAVKIKLKTKKK"
     gene            complement(946..>2839)
                     /locus_tag="VGA49_00650"
     CDS             complement(946..>2839)
                     /locus_tag="VGA49_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9664308.1"
                     /translation="LCDGIGHLIENPDATVEDLMQFVKGPDFPTKGIIYNKKEITNAY
                     ATGKGKVVIRAKTEIVEAKGGGYNILVTELPYQVNKAKLLEKIADLVTSKKIEGIRDL
                     RDESDKDGVRVVIELKKDSYPKKILNQLFKHTQLQDAFHFNMLALIDGLIPRVLTLKI
                     ILNEYIKHRQEVIKRRTQFELDKAQDRAHILEGLMIALADIDKIIALIKKSKDRDEAK
                     INLIKKFKLSERQAIAILEMRLQQLAGLERLKVETELKEKKQLIKELTAILKDPKKIL
                     NIIRDDLAHLKDKYGDERRTKIVPHAVDKFSMEDLIPDESTIIMVTQDGYIKRLAPET
                     FKTQARGGKGVIGLTTKEEDIVEHFFTSSTHADILFFTTTGRVFKLKAYEVPQASRTA
                     KGQAVVNFLQLSPGERVTAVRFLEKLKDKKYLVMATKFGLIKKVAIEQFTNVRRSGLI
                     AIKLKEGDLLNWVRMSTGNDDIMLVTKLGQAIRFKESDVRPMGRTAAGVRGLRLKKDD
                     EVVGMVVVSSKKLSKTSQLLIVMENGYGKRTDLHHYKIQKRGGSGIKTGKITSKTGQI
                     VAAHLMDLANLPEDEKGDLIVISDKGQVIRLPVKSVSVLGRDTQGVKIMRFKEQDDKV
                     ANVATA"
BASE COUNT          669 a          633 c          645 g          892 t
ORIGIN      
        1 acagagaaac agagaaacag aggaacagag aaactgagaa acagagaaac agagaaactg
       61 agaaacagag aaactgagaa acagagaaac aaaaactatt agttgcttag tcgctcagtt
      121 tttccgttct tcagttgctc agttcccttg tctttttgtt ctttagtttt gtttgtttct
      181 tcatcttttt gtttaatccc tttatcaagc aaatctttat aaacagcgcc caggacgcca
      241 ttgacaaatt tgccggatga ttcgccgcca aaagttttgg ccagttcaat cgcctcgtta
      301 atggcgactt tatcaggaat ggaattatca aaaattagtt cataaatccc aattcttaaa
      361 atatttcggt caacaatggt tatctgatcc agcggccatt ccggggcgta tttagtaatg
      421 gactgatcaa tttctttaat tttggcagta acgccctcca ggagatgttt ggtgaaaccg
      481 ccgtcgtcaa aatcaggcgc gaattcactc atgttgtgtt ttaaataggc attgagctga
      541 ttggccggcc gtccgttgaa atcccattca taaagcgtct gcatggcgat tgtccgggca
      601 agatgtctat tggacatagt ttattttttc ttggttttca attttatttt aaccgcttct
      661 cttcctttat aataaccgca atatttacag gcgtggtggg gctgaacagg cttctggcac
      721 tgggggcatt taaaaagttt tatttttttt aaagcaaaat gagaggctcg tttgcctttg
      781 gaggatttgg ttcttctctg tgttggaaca cccataaatt aaaattatta attatttaag
      841 ctatcataac taaaaatagc tgttttggca agatttttca agcccagccg gagatgttaa
      901 aatagatgac tgttctttcc agttcgcctt tcaatttgtt aaacgctagg ccgtggcgac
      961 attggccacc ttatcgtctt gttccttaaa tctcataatc ttgacgccct gagtgtcccg
     1021 gcccaaaaca gagacgcttt tgaccggcag gcgaatgacc tggcctttat cggaaataac
     1081 aatcaggtcg cctttttcgt cttccggcag attggccaaa tccatcagat gagccgccac
     1141 aatctggccg gtcttggagg tgattttacc ggttttaatc cccgagccgc cccgtttttg
     1201 aatcttgtaa tgatggaggt cagtccgctt gccgtaaccg ttttccatca cgatcaatag
     1261 ctgggaagtt ttagacagtt ttttggagct gaccaccacc atgccgacca cttcgtcgtc
     1321 ttttttgagg cggaggcccc ggacgccggc ggccgtccgg cccattggcc ggacatctga
     1381 ttctttaaag cggatagcct gacccagttt ggtgaccagc ataatatcgt cgttgccggt
     1441 tgacattctg acccagttga gcagatcgcc ctctttgagc ttaatggcga tcagcccgga
     1501 ccggcggaca ttggtaaatt gttcaatcgc tactttttta atcagcccga atttagtggc
     1561 catcaccaga tattttttgt ctttcagttt ttccaggaac cggacggcgg ttactctttc
     1621 accaggggac agctgtaaga aattgacaac cgcctggcct ttggccgtcc gggaggcctg
     1681 ggggacttca taggctttga gtttaaagac ccggccggtg gtcgtgaaga acagaatatc
     1741 agcgtgggtg cttgaggtaa agaaatgttc cacgatgtct tcttctttgg tggttagccc
     1801 gataacgccc ttgccgccgc gagcctgggt tttgaaagtt tccggcgcca gtcgtttgat
     1861 atagccgtcc tgggtgacca tgataatggt tgattcgtcg ggaatcaggt cctccatgct
     1921 gaatttgtca acagcatgag gaacaatttt tgtccgtcgc tcatcgccgt acttgtcttt
     1981 aaggtgggcc aggtcgtccc tgattatgtt taaaattttc ttggggtctt tcagaatagc
     2041 tgtcagctcc ttaatcagct gtttcttttc ttttagttca gtttcaactt tcagccgttc
     2101 caggccggct aactgctgga gccgcatttc cagaatggcg atcgcctgtc gctctgacag
     2161 tttgaatttt ttgattaaat taattttggc ttcatcccgg tcttttgatt tttttatcag
     2221 ggcgataatt ttatcaatat cagccagggc gatcattaac ccttccagga tatgggcccg
     2281 atcctgggct ttgtccagtt caaactgggt gcgtctttta ataacttcct ggcggtgttt
     2341 aatatattcg ttcaggatta ttttcagagt taaaacccgg gggatcagac cgtcaattag
     2401 agccagcata ttaaagtgaa aagcgtcctg gagctgggta tgtttaaaga gctgatttaa
     2461 gattttttta gggtagctgt cttttttgag ttcaatcacc acccgaacgc cgtctttgtc
     2521 cgattcgtcc cggaggtcgc ggatgccctc aattttttta ctggtgacca aatcagcgat
     2581 tttttccagc agcttggctt tgttaacctg ataaggcagt tcagtcacca gaatgttata
     2641 accgccgcct ttggcttcaa caatttctgt tttcgccctg ataaccactt tgcctttacc
     2701 ggtggcgtag gcgttggtaa tttctttttt attgtaaata atgcctttgg tcggaaagtc
     2761 cggcccttta acaaactgca tcagatcttc aacagtggcg tcaggatttt caatcaaatg
     2821 gccaatgccg tcgcaaagt
//
LOCUS       DASWHZ010000026         5830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_448997, whole genome shotgun sequence.
ACCESSION   DASWHZ010000026 DASWHZ010000000
VERSION     DASWHZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448997"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2680
                     /locus_tag="VGA49_00655"
     CDS             <1..2680
                     /locus_tag="VGA49_00655"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX9664309.1"
                     /translation="DSEQHIFVLQLKPGIDITGQDAGIVDLFNEDFARQSEFDWFNLT
                     SLSRSIKFLKEDLPIVKSKVVSTFPLSGSNEIQFEGSEFFTDNGNFKAKFFNGKKLEL
                     YFDDNQLVHYLDLGDLNKKIKPLYAEDKIIYNEIYQGIDIFYQAENERLKEYIIVKNE
                     EALLEFKFYYLIDSKHDLIQLPDGTISLFDTRGNTELLTMDLPAVYDSRDNSVEADMI
                     LGQVNYKDKQFKSITIQLNPTNQEIYYPLLIDPTYTIKTGVSPGDNRGRKLARTSDGS
                     LHVVYARSAGFGNEIFYAKSADNGQNWTETQITSYNDSDAQQREPSIAADFNDHLHLV
                     WSGQTFNLLYKYNIQYVQYTTSWSSPVFITAGNSHDQGAPSIAVDSTDNLHVVWDGLS
                     SAHPGVSNIQYSKYTDSWSSPGFITTDESYNQSNAALAVDSFDNLHVVWSGLSSTYPN
                     VTNIQYSKYTDSWSSPVFITTGNSHSQSSPSIAVDSSDNLHVVWNGLSSTYPNRNNIM
                     YSQYTDSWSSPIFITTGNNHHQFEPSLAIDFSDNLTVVWYGASATYSFDNIQYSQYTG
                     SWSSPGFLTEGNDAFKKYPNQIWALWPEIGGQRTNIPTTGFATVWTDISNLEIKYYSS
                     DDLSWVANTAPTAGTVTINPDPVNLSEGTTMAVTCSSTITDTDGFADISTASYATFYR
                     TSTGDPGEAGALDDNDSYRDNTASLSNCAGNTCDGQWTLTVQYFADPTDTGSIYAVDN
                     WTCAVVPKDATEAGVSASATVEMNTLTALSFDTSIGYGTMALGQDTGASPIATTITNT
                     GNEGIDVNFSGTDLTCDTGAIVVNNQEYSSISFTYGAGTPLTGTPTELDLDVPQRTDA
                     ASTATVYWGIGLPSSGVEGVCSGANSAAAVSDPNLD"
     gene            2829..3923
                     /locus_tag="VGA49_00660"
     CDS             2829..3923
                     /locus_tag="VGA49_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664310.1"
                     /translation="MSTKKSKQPEISDPQIFDNKFLWFFISVFVIILAVGLVKLSVNI
                     SPTQADDTTPSATVENEVPAASGVVFNTTGGTSVGASDGTPTDVSLTENTTTTVYCAG
                     TITDNNGCGDIQSSIAWLYRVGGSGTLAAVDCDADLEVDNDNCYKATNCAIDTDVNAC
                     SGISDFTASYKCSVAMQYFAQSTDAGSYQENVDGNGANDDANWKCTLVPSDESLGTSA
                     ESAAGKEVNSLTALNIASTIAYGTLALGADTGATNQTTLVTATGNVGIDVNLSGTDMT
                     CDTGSILVGNQEYASATFTWGVGGTDLSGGATELDLDVLKHNSADTNDDDKDVFWGLG
                     APATGAEGACSGTNTAAAVEDTNCGYTTCD"
     gene            3971..5182
                     /locus_tag="VGA49_00665"
     CDS             3971..5182
                     /locus_tag="VGA49_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664311.1"
                     /translation="MKYKNLLKAIFALTIFFGAAGVVQAVSLGISPSRVVVDHLLRGS
                     HYEKTIVLSRSDASQALNFQVDFEGEAADWVSTNYGRSFTIPTEVQRFPVNVMVDVPA
                     DTANGTYEGRLVFKGAPTETTRPEGQNVVSFNVNAILNLELTVVGDQVLDYQVSTVKV
                     PNIEEGTPISVYLVVQNNGNVAARPTRLQVDFYDKFQKVLLDSVSVTEMNSVEAFRTG
                     NVIADINTNLGVGQYWAFIKVYRDGQVIMETKVPFDILEIGSLEKRGEFRELLFKRQV
                     NVGEVQKFTGIFENLGATPVFAKLVLEISRGDQLVDVVEGDRIRVERGKTGNTVAYYT
                     PSTAGVYSVRAHINYDNKTTDVKDAQMVVRGIVTSMIVTAIETQGLLILVILLLIIIA
                     VALHYSKKRKK"
     gene            5305..>5830
                     /locus_tag="VGA49_00670"
     CDS             5305..>5830
                     /locus_tag="VGA49_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9664312.1"
                     /translation="MLVIFIIAGQSLKADTVGTEVFIVPFLPVPTPLGPAPDRHPPVI
                     FNVFIKNVTFDGATITWNTNEKARTVFHYGLTQQYGQGSIINDQFLTSHQVILSDLFS
                     NKTYHFQIVAFDKNNNQTTSDDYFFETLEVVDDIPPGNIRVFDSEVTADNQAIRLTWL
                     NPPDEDLRGIKIQRR"
BASE COUNT         1597 a         1261 c         1314 g         1658 t
ORIGIN      
        1 agattcagaa caacatattt ttgttctcca gctcaagccg gggattgata tcaccggcca
       61 ggacgccggc attgttgatt tattcaatga agattttgcc aggcagtctg aatttgactg
      121 gtttaattta accagcctct ccagatcaat taaatttttg aaagaagatt taccgattgt
      181 aaaatcaaaa gtagtttcca cttttccttt atccggcagt aacgaaatcc aatttgaagg
      241 cagtgaattt ttcacggata atggtaattt taaagccaaa ttttttaacg gaaaaaaact
      301 ggaactgtat tttgacgata atcagctggt tcattatctt gatctgggtg atttaaataa
      361 aaaaattaag cctttatacg ctgaagacaa aataatttat aacgagattt atcaggggat
      421 agatatattc tatcaggctg aaaatgaacg gctgaaagaa tatattatcg ttaaaaatga
      481 agaagctttg ctggagttta aattttatta tctaattgac agcaagcatg atttaattca
      541 actgccggac gggacgattt ctttgtttga taccagaggt aacacggaac tcttaaccat
      601 ggatttaccg gcagtctatg atagtcggga taattcagtg gaagcggaca tgattttggg
      661 ccaggtcaat tacaaagata aacagtttaa aagcatcacc attcagctaa atccaactaa
      721 tcaggaaatt tattatcctt tattaattga cccgacgtac acgatcaaga ccggagtgtc
      781 tccgggcgac aacagaggac gcaaattagc cagaaccagc gatggcagtt tgcatgttgt
      841 ttatgctcgt agtgccggtt ttggtaatga aattttttat gccaagtcag ctgataacgg
      901 tcaaaattgg actgaaacac aaattacttc ctataatgat tcagacgccc aacagcggga
      961 gccctccatt gccgctgatt tcaatgacca tttgcatctg gtttggtccg gacaaacttt
     1021 taatttgcta tacaaatata atattcagta tgtccaatac actacttcct ggagtagtcc
     1081 tgtttttatc accgccggca atagccacga ccagggggct ccatccatag cagtagattc
     1141 cacagacaat ttgcatgttg tttgggatgg cttaagttca gctcatcccg gcgtttctaa
     1201 tatccagtat tcaaaataca cggattcctg gagcagcccc ggctttatca ccaccgatga
     1261 gagctataat caatctaacg ccgctctggc cgttgattcc tttgataatt tgcacgttgt
     1321 ctggagtgga ctaagttcaa cttatcccaa cgtcactaat attcagtatt ctaaatacac
     1381 tgattcctgg agtagtcctg tttttatcac caccggcaat agtcatagcc agagttcccc
     1441 gtcaattgcc gttgattcct ctgataattt gcatgttgtt tggaatggac taagttcaac
     1501 ttatcccaac cggaataata tcatgtattc ccaatatact gattcctgga gtagtcctat
     1561 ttttatcact accggtaata atcatcatca atttgaaccg tctttggcaa tagatttttc
     1621 tgataatttg actgtggttt ggtatggagc aagcgcgact tatagttttg acaatataca
     1681 atactctcaa tatactggtt cctggagcag tcctggtttt ttaactgaag gtaatgatgc
     1741 tttcaaaaaa taccctaatc aaatttgggc tttatggcct gaaattggcg gccagcgaac
     1801 aaatattcca acgactggat ttgccactgt ctggaccgac atctccaatc ttgagatcaa
     1861 atattattca tctgatgatt tatcctgggt ggctaatacc gccccaacag ccggaaccgt
     1921 taccattaat cccgaccccg ttaatttaag cgaagggacg actatggctg tcacttgctc
     1981 ctcaaccatt actgacaccg acggctttgc tgatatcagt actgcctctt acgctacctt
     2041 ttaccggaca tcaaccggcg atccgggcga ggccggcgct ctggacgaca atgacagcta
     2101 ccgggataac acggcaagtt taagcaattg cgccggcaat acctgcgatg ggcagtggac
     2161 tttaactgtc caatattttg ccgacccaac tgacaccggt tcaatttacg ctgtggataa
     2221 ctggacctgc gctgtcgtgc ctaaggacgc cactgaagcc ggcgtttcgg ccagcgccac
     2281 cgtggaaatg aatacgctga ctgctcttag ttttgatacc tcaatcggat acgggacgat
     2341 ggctttgggc caggatacgg gcgccagtcc aatagccacg accattacca ataccggcaa
     2401 tgaggggatt gatgtcaatt tcagcgggac tgatttgacc tgtgataccg gcgcgatcgt
     2461 cgttaataat caggaataca gctctatttc ctttacttat ggcgctggaa cgcctttgac
     2521 tggaacgccg acagaacttg atttggatgt ccctcaaagg acagacgccg catccaccgc
     2581 tacggtttac tggggcattg gcctgccgtc atcgggagta gaaggcgttt gcagcggcgc
     2641 taattcagcc gcggctgttt ctgatcctaa cctggattaa ctaccttctt atgccccctt
     2701 tgattccctc tgattccctc tgattccttc ttatcttctt atccccttta ccccttttat
     2761 tttcaatgtt ataattaaaa aaataaaaga tacttatcca caatataaaa ttattaattt
     2821 aaaaaaagat gtctacaaaa aaatcaaagc aaccggaaat ttcagatcca caaatctttg
     2881 ataacaagtt tttgtggttt tttatttctg tttttgtgat tattctggcg gttggtctgg
     2941 tgaaattgtc cgtcaatatc agcccaactc aggctgacga caccacgcca tcagccactg
     3001 ttgaaaatga agtgccggcg gccagcggag tggtttttaa cacgaccggt gggactagcg
     3061 ttggcgcctc cgacggcacg ccgacggatg ttagcctgac cgaaaatacc acgaccacag
     3121 tttattgcgc cgggacgatt actgataata acggctgcgg tgatattcaa agctccattg
     3181 cctggcttta ccgcgtgggc ggttccggca ctttagcggc ggttgactgc gacgctgatc
     3241 tggaagtcga taatgataac tgctacaaag cgaccaattg cgctattgat actgatgtta
     3301 acgcctgcag cggcatcagc gattttacgg ccagctataa atgttccgtg gctatgcagt
     3361 atttcgctca atcaaccgac gccgggtctt atcaggaaaa tgttgacggc aacggcgcca
     3421 atgatgacgc taactggaaa tgtactctgg tccccagcga cgaatcactt ggcaccagcg
     3481 cggagtcagc cgccggcaaa gaggttaatt ctctgacagc cttaaatatt gcttcaacta
     3541 ttgcctatgg tactttggct ctaggggcgg ataccggcgc gaccaaccaa actactttgg
     3601 taacagctac cggtaatgtt ggcattgacg ttaatttaag cggaactgac atgacttgtg
     3661 ataccggcag tattcttgtt ggtaatcagg aatacgccag cgccaccttt acctggggtg
     3721 ttggcgggac tgatttatca ggcggcgcca ctgaacttga tcttgatgtc ttaaagcaca
     3781 attcagctga taccaatgat gatgacaagg atgttttctg gggactgggc gcgccagcaa
     3841 ccggagcgga aggcgcttgt tccggaacta acacggccgc ggctgttgaa gacactaatt
     3901 gcggctacac aacctgcgat taataaatta aattaaaatt aacataaaat aaatgccgga
     3961 ggtaaataaa atgaaataca aaaatttatt aaaagcaata ttcgccctga cgattttctt
     4021 tggggcggcg ggggtggtcc aggcggtcag tctggggatc agtccttccc gggtggttgt
     4081 tgatcactta ttgcgcggtt ctcattatga aaagacgatt gttttaagcc ggtcagacgc
     4141 cagccaggct ttgaattttc aggttgattt tgaaggcgaa gccgctgact gggtttcaac
     4201 taactacggc cgaagtttta cgattccgac tgaagttcag cgttttccgg taaatgtgat
     4261 ggttgacgtg ccggctgaca cggctaacgg cacttatgaa ggacggctgg ttttcaaagg
     4321 cgcgccgact gaaacaaccc gtcctgaagg acaaaatgtc gttagtttta atgtcaacgc
     4381 tattttgaat ttagagctga cggttgtcgg cgatcaggtt ttggattatc aggtctcaac
     4441 agtcaaagtg cccaacattg aggaagggac gccaattagt gtttatttag tcgttcagaa
     4501 taatggtaat gtcgccgccc ggccaaccag attacaggtt gatttttacg ataaattcca
     4561 gaaagttttg cttgattcag tcagcgtgac tgagatgaat tcagttgaag ctttccgaac
     4621 cggcaatgtg atagctgata ttaacactaa cctgggagtc ggccagtact gggctttcat
     4681 caaagtttat cgcgacggcc aggtgattat ggaaaccaaa gtgccttttg atattttgga
     4741 aattggcagt ctggaaaaaa ggggtgaatt tagggaatta ttattcaaaa gacaggttaa
     4801 tgttggtgaa gttcagaaat ttacgggtat ttttgaaaat ctgggcgcca ctccggtttt
     4861 cgccaaatta gttttggaaa tttcccgggg agatcagctg gttgacgttg ttgagggcga
     4921 ccggattaga gtggaaagag gcaaaaccgg caacaccgtg gcctactaca ctccgtcaac
     4981 agccggtgtt tattcggtcc gggcccacat taattacgat aataaaacaa ctgacgttaa
     5041 ggatgcccag atggtggtcc ggggaatcgt caccagtatg attgtcacgg cgattgaaac
     5101 ccagggcctg ctcatcctgg ttattctctt actgattatt attgccgtcg ctcttcacta
     5161 ctccaaaaaa agaaaaaagt aaccagacag attaagcttc aggaacagcc ccgttggtca
     5221 cgactgacgg ggctgtttag tttggttttt ttatagtata atgaccgtaa gatgatcaaa
     5281 aaaattagta tcttaattat ttgcttgctg gtaatattta taatcgccgg gcagtcgtta
     5341 aaagcggata cggtcggcac agaagttttt attgttccct ttttaccggt gccaactccg
     5401 ctggggccgg cgcctgaccg tcatccgccg gtaattttta atgtttttat taaaaatgtt
     5461 acttttgacg gcgcgaccat tacctggaat actaatgaaa aagccaggac agtttttcat
     5521 tatgggttaa ctcaacaata tggtcagggg tcaataatta atgatcagtt tctgaccagc
     5581 caccaggtta ttttatcaga cttattttcc aataaaacat atcattttca gatagtcgct
     5641 tttgataaaa ataataatca gacaacttca gatgattatt tttttgagac cctggaagtg
     5701 gttgatgata ttccgcccgg caatatcagg gtttttgatt cggaagtaac cgctgataat
     5761 caggctatcc ggctgacctg gctcaatccg ccggatgaag atttacgagg aattaaaatt
     5821 cagagacgaa
//
LOCUS       DASWHZ010000027         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1183515, whole genome shotgun sequence.
ACCESSION   DASWHZ010000027 DASWHZ010000000
VERSION     DASWHZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183515"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VGA49_00675"
     CDS             complement(<1..826)
                     /locus_tag="VGA49_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9664313.1"
                     /translation="MFIKKIGIDLGTTNILVYVPKRGIVINEPSVVAISTQDKKVLAV
                     GQEAKEMLGRTPDTIVASRPLKDGVIADYKTTEAMLRYFINKALGGIRFFRPEVMVAV
                     PAGITSTERRAVIDATIAAGAKAAYIIKEPVAAAIGANIPIGSASGHMIIDIGGGTAE
                     MAVISLGGIVSSTSVRVGGNKFDYAISEYIRKKYGLAIGDRTAEQVKIKIGSALYLED
                     KLYLDVRGRDMISGLPRTINVSSDDVTDAIQNELEAIILAVKDVLYKTPPELSADVI"
     gene            complement(962..2896)
                     /locus_tag="VGA49_00680"
     CDS             complement(962..2896)
                     /locus_tag="VGA49_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX9664314.1"
                     /translation="MEFEQIQKEAGAESKFKKFLKIVLVVFFIVAVLSLYGLAVFYLL
                     NQAQDQALAGRDQLSLARQNVTDRDFGQATENFNQANSLFKKARRGVYPAYPLVIVPR
                     VGSNVLAASRLLSAGINTTDALTIGSRIADEILTELKASKDLNYNDLTPSQKTFIISK
                     IYNSQEDFREISDKITGAFEQVQKIDRDKVVDQISRAKEEFEIQLTGIKQTVDEVIPF
                     TVILPQLAGYPETKTHLVLLQNSTELRPTGGFIGNYSVIKIQNGQILSLMTDNTYSLD
                     SPMEGILQVEPPDPIKKYLEVPDWFMRDSNWSPDFKISAQKAEEFYHLEHGPEDHFDA
                     IIGVTPELVKGFLRITGPQRVEDEVYTTDNFEDALEYRVSVTAFQLGEDFLKRKRVIN
                     TLTQQIRDDLFNLPLNHWLDIVAVMGRALEEKDLMIYEKDKDLQTVIANNNWSGELKQ
                     IEADYLMVVDANLGALKTDPFIDRTIDYSLTENDSGDYIAKVALTYNNRAGFSRTTTR
                     YRTYTRVYAPLGSEIISQSGLMASDRSAQPGQLEVGQELNKTYFGGFISIEPGQTGQL
                     VFEYQLPSEIKRKILSDQYQIYIQKQPGTRQTKLNVDLNFRNKIKSYQPIFEGAKRLD
                     DNHLRFEGDLRVDREYRINF"
     gene            complement(2898..>3323)
                     /locus_tag="VGA49_00685"
     CDS             complement(2898..>3323)
                     /locus_tag="VGA49_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664315.1"
                     /translation="RTVQQSCLNFPSLLTPIYSRTILGRFGRGKKILADYRMQQFDHN
                     QTREVDWVISSCMLIRKDFLDKHGLLDERFFVYFSDVDLCRRCWQNNYQVAYFCDTNI
                     IHYYHRESAENQGLKVFFSPITRIHIKDWLKYLIKYKGQ"
BASE COUNT          923 a          790 c          642 g          968 t
ORIGIN      
        1 caatcacatc agctgacaat tcaggtggag ttttgtacaa aacatcttta accgccagaa
       61 taatcgcttc cagttcgttc tgaatggcgt cagtcacgtc gtctgaactg acattaatgg
      121 ttctgggcaa gccactaatc atatcccggc ccctgacatc cagatagagc ttgtcttcca
      181 gataaagagc tgagccaatt ttaattttga cctgttcagc cgtccggtcg ccaatggcca
      241 gcccgtattt tttacgaata tattcagaaa tagcgtagtc aaacttattg ccgccaaccc
      301 ggacggaagt gctggaaacg atccccccca aagaaataac ggccatttca gcggtgccgc
      361 cgccaatatc aataatcata tgaccggaag cgctgccaat cggaatattg gcgccaatag
      421 cggccgccac cggttcttta atgatgtagg cggccttggc gccggcggcg atcgtggcgt
      481 caatgaccgc tcttctctcc gttgaagtaa tcccggccgg cacggccacc atcacttccg
      541 gtctaaaaaa cctgatgccg cccagagcct tattgataaa ataacgcagc atcgcttcag
      601 ttgttttata atcggcaatc accccgtctt tcagcggccg ggaagcgaca atcgtgtccg
      661 gcgtccggcc gagcatttct ttggcttcct ggccaacagc cagcactttt ttgtcctggg
      721 tggaaatagc cacaaccgac ggctcgttga taacgatccc tcttttgggc acgtaaacca
      781 ggatattggt agtgccgaga tcaattccga ttttctttat aaacataaac tataaaattt
      841 ctaaatccta attcctaatt tctaaacaaa tcccaatttc taatgttcca atgtcaaaaa
      901 cgtaatttag aattttgaaa attggctcat tagacattgt ttagaattta gatattagat
      961 attagaaatt aattctgtat tcccgatcaa ccctcaaatc tccctcaaat cttaaatggt
     1021 tatcatccag ccgcttcgcc ccttcaaaaa ttggctggta tgattttatc ttatttctaa
     1081 aatttaagtc aacattaagt ttagtctgac gcgtccccgg ctgtttctga atataaatct
     1141 ggtactgatc agataaaatt tttcgtttta tttctgacgg cagctgatat tcaaaaacca
     1201 gctggccggt ctggcccggt tcgattgata taaaaccgcc aaagtaagtt ttatttagtt
     1261 cctgacccac ttccagctgg cctggttgag ccgatctgtc actggccatc aaacctgact
     1321 ggctgatgat ttctgatcct aaaggcgcgt aaactctggt ataagtccgg tatctggtgg
     1381 tcgtccggct aaaacccgcc cggttgttat aagttagcgc gactttggcg atataatcgc
     1441 cgctatcatt ttcagttaag ctgtaatcaa tcgtccggtc aatgaatgga tcagttttta
     1501 aggcgcctaa attagcgtca acgaccatta agtaatcagc ctcaatctgt ttcagctcgc
     1561 ctgaccagtt attattagcg ataactgttt gaagatcttt atctttttca taaatcatta
     1621 aatccttttc ttccaaagcc cggcccatga cagccacaat gtccagccag tgattcagcg
     1681 gtaaattaaa aaggtcatcc ctgatttgct gggttaaagt attaatgact ctcttccttt
     1741 tcaaaaaatc ctcgcccagc tgaaaagcgg tcactgatac ccggtattcc agggcgtctt
     1801 caaaattgtc agtcgtgtaa acttcatctt caactctttg cggtccggta atccttaaaa
     1861 atcccttaac caattctgga gtaacgccaa tgatcgcgtc aaaatgatct tccggtccgt
     1921 gctccagatg ataaaactct tcagcttttt gagcggaaat tttgaaatca ggcgaccagt
     1981 tggaatcgcg cataaaccag tccggcactt ccagatattt ttttatcggg tcaggcggtt
     2041 caacctgtaa aataccttcc attggcgaat ccaaactgta agtattgtct gtcattaaag
     2101 ataaaatttg tccgttttga attttaataa ccgaataatt tccaataaaa ccgccggtcg
     2161 gccttaattc agtgctgttt tgaagcagaa ccaaatgagt tttagtttcg ggataacctg
     2221 ccagctgggg cagaataacg gtaaacggga taacctcgtc aactgtctgt ttgataccgg
     2281 tcagctgaat ttcaaactcc tctttggccc ggctgatttg atcaacaact ttgtcccggt
     2341 caattttttg aacttgctca aaggccccgg tgattttatc actgatctcc ctgaaatctt
     2401 cctgtgaatt ataaatctta ctgataataa aagttttttg gctgggtgtt aaatcgttat
     2461 aatttaaatc tttactggct ttcagttctg tcagaatctc atcagcgatt cttgaaccaa
     2521 tggttaaagc gtcggttgta ttaatcccgg ctgacaataa acgactggcg gccaaaacat
     2581 tagatccaac ccggggaaca atcaccagtg gataagccgg ataaactcca cggcgggctt
     2641 ttttaaataa actgttggcc tggttaaaat tttctgtcgc ctgaccaaaa tctctgtcag
     2701 taacattttg ccgggccaat gacagctgat cccggccggc cagagcctga tcttgagcct
     2761 ggttcagcaa ataaaagacg gccaggccgt ataaacttaa aacagcaaca ataaaaaaaa
     2821 cgactaaaac tatcttcaaa aattttttga atttagattc cgcgcctgct tctttttgta
     2881 tttgctcaaa ttccatatta ttgacctttg tatttaatta aatattttaa ccaatcttta
     2941 atatgtatcc gggtaatcgg actaaaaaaa actttcagtc cctgattttc agccgactcc
     3001 cggtgataat aatgaataat gttcgtgtca cagaaataag cgacctgata attattctgc
     3061 cagcaccgtc ggcataaatc aacgtcagaa aaataaacaa aaaatctttc atccagcagg
     3121 ccgtgcttgt ccaaaaaatc ttttctgata agcatgcagc tgctgataac ccagtcaacc
     3181 tccctggtct gattatggtc aaactgctgc atccggtaat cagccagtat ttttttaccc
     3241 cggccaaacc ttcccaaaat tgtccgggaa taaatcggcg tcagcagtga cggaaaattc
     3301 aggcaggact gctgaactgt ccg
//
LOCUS       DASWHZ010000028         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3398070, whole genome shotgun sequence.
ACCESSION   DASWHZ010000028 DASWHZ010000000
VERSION     DASWHZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3398070"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VGA49_00690"
     CDS             complement(<1..570)
                     /locus_tag="VGA49_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX9664316.1"
                     /translation="MMSILSKIFGDPNEKYLRKLQPLVDQINALEKEFETLSDEELKK
                     KTEEFKEQLNQGKTLDDILPEAFAVVREAAKRTLNQRHYDVQLLGGIVLHQGQIAEMK
                     TGEGKTLTSTLAVYLNALAGQGVHVVTVNDYLAKRDAEWMGQVYDLLGLTIGCIQNQN
                     VSFLYNPGLTQTEDQVRDRGVEIVESALQP"
     gene            complement(711..1157)
                     /gene="raiA"
                     /locus_tag="VGA49_00695"
     CDS             complement(711..1157)
                     /gene="raiA"
                     /locus_tag="VGA49_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9664317.1"
                     /translation="MKITIKGTNIKLTPEIKDYIDQKLNSLDKFVKGILEALVEVGKT
                     SQHHRTGQVFRAEADLRLPGKVLRAEAVAGDLNTAVNQVKDELQRQLKKYKNRQEAKS
                     RRGQRVLKRELHLDPRAKSNREKKSAEGGSASGGKGGRELDEGSLQ"
     gene            complement(1183..2175)
                     /gene="murB"
                     /locus_tag="VGA49_00700"
     CDS             complement(1183..2175)
                     /gene="murB"
                     /locus_tag="VGA49_00700"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9664318.1"
                     /translation="MPNMKSIDNKIVKNKSLAQFTNYKIGGPARYFLAVKKDQDLVEA
                     IKWAKENKIEYFILGGGTNILISDDGYAGLVLKLENKDFTIKDNVIKTGAGAILFDLV
                     KASVAQGLTGFEWASGLPGTVGGAVRGNAGAFGGQMDQIVTNAKAFDCQTLKFNEYNN
                     KQLQFSYRHSRFKEEVNQEIVLSVEIKLKKGGDKKKSQMMIAEYLEKRGKLPILPSAG
                     CIFKNVELKKGTFQLADPGQGEIKETVKKDIRRDHPEYLEWGKIPAAFLIEYVGLKGK
                     QINNVQVSDKHANYLVNLGGGRAADVIMLISLIKQKVRSEAGIQLKEEVQLVGF"
     gene            complement(2279..>2947)
                     /locus_tag="VGA49_00705"
     CDS             complement(2279..>2947)
                     /locus_tag="VGA49_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9664319.1"
                     /translation="LKSALEKSLKRLRTDCLDLIQIHNPTLEDIERGEVFELFQDFQK
                     QGLIKFWGVSLRQPSDGLKAIAKASQLRVDLATIQVAFNVFQRQAKDKLFELADKHNV
                     GLIVREPLCRGFLSGKYDIAEDFETHAVVKFLNENKALPEDILKAFDQVQKIFKKYNR
                     PEDPVQLALKYILSFNQVGTVIPGAKNISQARQNLTSFELPDFEPELLAELNNFSQQF
                     AYTM"
BASE COUNT          795 a          675 c          559 g          918 t
ORIGIN      
        1 gggctgaaga gctgactcaa caatttccac tccccggtct ctaacctgat cttccgtttg
       61 agttaagcct ggattgtaaa gaaaggaaac attttgattc tgaatacaac caatggtcaa
      121 tcccagcaaa tcataaacct ggcccatcca ctcggcgtct ctttttgcca gataatcatt
      181 aacagtcacc acgtgaactc cctgaccggc caaagcgttt aaataaacag ctaaagttga
      241 cgtcaaagtt ttaccttcac cggttttcat ttcagcgatt tggccctgat gcaaaacaat
      301 gccgcccaaa agctggacat cataatgccg ctggttgagc gttcttttgg cggcttcccg
      361 gacaacggca aaagcctccg gtaaaatatc gtccagagtt tttccctgat ttaattgctc
      421 cttaaattct tcggtcttct tttttaactc ctcatctgat aaagtttcaa attccttttc
      481 cagggcatta atctggtcaa ccagcggctg gagcttgcgt aaatattttt cgtttggatc
      541 gccgaatatt tttgataaaa tagacataat aatggaataa gtcaaaactt aaaagtcaaa
      601 agtagaagtt aaaagtaaaa aaattaataa aaaatattac ctaacttttt acttcttact
      661 ttttactttt gacttattga ttgatcagca attataaaat aaaatcaatt ttattgcaag
      721 cttccttcat ccagttcccg gccgcctttt ccgcccgagg cggatccgcc ttcggcggat
      781 ttcttttcac gatttgattt ggcccgggga tccagatgaa gctcccgctt caaaactctt
      841 tggccgcgcc ttgatttagc ttcctgccgg ttcttgtatt ttttcagctg ccgctgaagt
      901 tcgtctttaa cctgattaac agccgtatta agatctccgg cgactgcttc agcccttaaa
      961 accttcccgg gaagtcttaa atcagcctct gcccgaaaaa cctggcctgt ccggtgatgc
     1021 tggctggttt tgccaacttc caccaaagct tccaaaatgc ctttgacgaa tttatcaagg
     1081 ctgtttagct tttgatcaat gtaatcctta atttccgggg tcaatttaat atttgtgcct
     1141 ttgatggtta ttttcatgta attgttacag gaataattat ttttaaaacc cgaccagctg
     1201 gacttcttct tttaattgaa tcccggcttc tgaccggact ttttgcttaa tcagactgat
     1261 cagcataatg acatcagccg cccgaccgcc gcccagatta accagataat tggcgtgttt
     1321 atctgagacc tgaacattat taatttgttt gcccttgagg ccaacatact ctatcaaaaa
     1381 agcggccgga attttgcccc attccagata ttccgggtga tcccgcctaa tgtctttttt
     1441 aactgtttcc ttaatttcac cctggccggg atcagctagt tgaaatgtcc cttttttcag
     1501 ttcaacattt ttaaaaatac aaccggcgct gggcaagatt ggaagttttc cccgcttctc
     1561 cagatattca gcgatcatca tctggctttt tttcttatcc ccgccttttt tcagttttat
     1621 ttcaactgac aaaacaattt cctgattgac ttcttcttta aaccggctgt gtcggtagct
     1681 aaattgaagc tgtttattat tgtattcatt aaatttcaga gtctgacagt caaaagcttt
     1741 ggcgttggtg acaatttgat ccatctggcc gccaaaagcc ccggcattgc cccggacggc
     1801 cccgccaacc gtgccgggca ggccggaggc ccattcaaag ccggttaaac cctgggcgac
     1861 tgacgctttg accagatcaa ataaaatcgc gccggcgcct gttttaataa cattatcttt
     1921 gattgtaaaa tctttgttct caagttttaa aaccagtccg gcatagccgt catcagaaat
     1981 taaaatattg gtgccgccgc ccagaatgaa atattcaatt ttgttttctt tagcccactt
     2041 aatggcttcg actaaatcct gatctttttt aacggccaaa aaatatctag ccggaccgcc
     2101 gattttatag ttagtaaatt gagccagtga tttattttta acaattttat tatcaatgct
     2161 tttcatgttt ggcatataaa tcaggggtga tgagggttga tgaaggaaga tgaagggaga
     2221 tgaaggggag gagatcaggg gttaggtaat tataaaatta tttgtctctt ttggaaaatt
     2281 acatcgtgta ggcaaactgc tgcgaaaaat tattgagttc agctaaaagc tcgggttcaa
     2341 aatctggcag ttcaaaagag gttaaatttt ggcgggcttg gctgatattt ttggcgccgg
     2401 gaataactgt tccgacctga ttaaaagaca aaatatattt taaagccagc tggaccggat
     2461 cttccggccg gttatacttt ttaaatatct tctgaacctg atcaaatgct tttaaaatat
     2521 cttccgggag agctttattc tcatttaaaa atttgacgac ggcgtgagtt tcaaaatctt
     2581 ccgcgatgtc atatttcccg cttaaaaatc cgcggcataa aggctcccgg acaatcagcc
     2641 cgacattatg cttatcggcc agctcaaaaa gcttatcttt cgcctgacgc tgaaaaacat
     2701 taaaagcgac ctgaatcgtc gccagatcaa ctcttagctg gctggccttg gcaatcgctt
     2761 ttaaaccgtc agacggttgc cttaaagaca ccccccaaaa tttgatcaga ccctgctttt
     2821 gaaaatcctg aaacagctca aaaacttcac cccgttcaat atcctccaaa gtcggattat
     2881 gaatttgaat caagtcaagg cagtctgtcc gcagtctttt caagcttttt tcaagagctg
     2941 actttaa
//
LOCUS       DASWHZ010000029         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_80241, whole genome shotgun sequence.
ACCESSION   DASWHZ010000029 DASWHZ010000000
VERSION     DASWHZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80241"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGA49_00710"
     CDS             complement(<1..371)
                     /locus_tag="VGA49_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9664320.1"
                     /translation="MSKILGIDLGTTNSAMAIIEGGEPKILENKEGNRTTPSMVAISK
                     NGERLAGQLAKRQAVTNPENTLFSIKRLIGRRFEANEVQKDIKTMPYKIVKAGEGVKV
                     VMNGKEYTPQEISAMILQKLKA"
     gene            complement(404..841)
                     /locus_tag="VGA49_00715"
     CDS             complement(404..841)
                     /locus_tag="VGA49_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9664321.1"
                     /translation="MPIIKWNPFGDWPEDFDLDRFFQTPMVPQKMQGFIPSIDVYQDN
                     GHIVAETSLPGVDPKDVEVSIENDMLTIKGKTEKKTEVEEKNYFRKEMRSGVFHRRVA
                     LPAHVLGDQASADFENGILKITIPKAEPKKSKKVEIKVKKSRK"
     gene            complement(960..1433)
                     /locus_tag="VGA49_00720"
     CDS             complement(960..1433)
                     /locus_tag="VGA49_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9664322.1"
                     /translation="MEEDKKQQSKEIQDLQQKCEEYLNGWKRAKADYINLKKETDKKQ
                     AEIMRFAAANFFMSLLPIIDNVKQAISHIPDAEKEKDWVEGFINIKKQFDELLTNLRI
                     EEIKTTGEKFNPEFHEAVSKEKVEGKEEGIVVKEVSGGYMIEDKVIVPAKVVVSE"
BASE COUNT          409 a          385 c          267 g          572 t
ORIGIN      
        1 gctttgagtt tctgcaaaat catcgctgaa atttcctgcg gcgtgtattc tttaccgttc
       61 atcacaacct tgactccttc gccggcctta acaatcttat acggcatggt tttaatgtct
      121 ttttgaactt cattagcctc aaaccgccgg ccaattaacc ttttaatgga aaataaagta
      181 ttttccggat tggtgacggc ctgtcttttg gccagctggc cagccagtcg ctcgccattt
      241 ttggaaatag cgaccatgga cggggtcgtt ctattccctt ctttattttc taaaattttt
      301 ggctcaccgc cttcaataat ggccatggcc gagtttgtcg tgccaagatc aattcctagt
      361 attttactca taatttttag tcgtgaatta gttattcggc aatttacttc ctactttttt
      421 tgactttaat ctccactttc tttgacttct tcggttcagc tttggggatg gtgattttca
      481 aaataccgtt ttcaaaatcg gctgaagcct ggtcgcccag aacatgagcc ggcaaagcca
      541 cccgccggtg aaaaacacct gaccgcattt cttttctgaa gtaatttttt tcctcaactt
      601 cggtcttttt ttcggtcttg cctttaatag tcagcatgtc gttttcaatg gagacttcga
      661 cgtctttggg atcaacgccc ggcaaagacg tttcggccac gatatggccg ttatcctgat
      721 aaacgtcaat tgacggaata aatccctgca ttttttgcgg gaccattggc gtctgaaaaa
      781 accggtccag gtcaaaatct tccggccagt cgccaaaagg gttccatttg atgataggca
      841 tatttttatc tccatagttc gaacatattc aatgttcggg gtttattaat taatagatta
      901 aagatttaag attaaagatt taagtagctt aaatcttaca acttacaact taaatctaat
      961 tattcgctaa caaccacctt cgccgggaca atcactttat cctctatcat ataaccccct
     1021 gatacttctt taacgacaat cccctcttct ttcccttcaa ctttttcttt ggaaaccgct
     1081 tcatgaaatt ccggattaaa cttttcgccg gttgttttaa tttcctcaat ccgcaaattt
     1141 gttaaaagct cgtcaaactg ttttttgata ttgataaacc cttccaccca gtccttttct
     1201 ttttcagcat caggaatatg gctaatggcc tgtttgacat tatcaataat cggcagtaac
     1261 gacatgaaaa aattagccgc tgcaaatcgc atgatttcag cctgtttctt gtcagtttcc
     1321 ttttttaaat taatataatc agccttggcc cgcttccagc cgtttaaata ttcttcacac
     1381 ttttgctgaa gatcctgtat ctctttggat tgttgttttt tatcttcttc cataaaggat
     1441 taaaagccat tagtcactag tcatcagcca ttagtatccc tgtgttgact gttgactgtt
     1501 gactattgac taacctcaat gcttaacctc cagatcatat aatatctctt caatcttctt
     1561 caaattccgt ttaacctcct catatttatt ccttaacttg ccaaccagat caaactgaat
     1621 cagctgttcc acg
//
LOCUS       DASWHZ010000030         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_82663, whole genome shotgun sequence.
ACCESSION   DASWHZ010000030 DASWHZ010000000
VERSION     DASWHZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82663"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VGA49_00725"
     CDS             complement(<1..229)
                     /locus_tag="VGA49_00725"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX9664323.1"
                     /translation="MRKEKGQLIVALDVETFDEACQLLNLLKDDVDIFKVGSQIFTAC
                     GTRIVHFIHESGKKIFLDLKFHDIPNTVANAV"
     gene            complement(226..1131)
                     /locus_tag="VGA49_00730"
     CDS             complement(226..1131)
                     /locus_tag="VGA49_00730"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9664324.1"
                     /translation="MNIAVTIGKIKFKNPVAVASGTFGHSEKYYNLEEVKKLGALTPK
                     TVTLKAQEGNPPPRIVETPSGMLNAIGIENPGADAFIQAKLPLFKGIGIPIIISILGH
                     TDREFTVLAQKFNKAGGVSVLELNLSCPNLGKKVLVAQDPKATYRVVKAVKKISDYPV
                     IAKLAPNVTDVTTIAKAAEDAGADGVSLINTFSGMVIDVHKRASVLGNVTGGVSGPAI
                     RPMAVDMVYKTAQKIKIPIVAMGGITTGEDALQYIIAGATMVAVGTANFINPRAPLDV
                     LNGIISYMRKNKVTDIHKLIGCVKS"
     gene            complement(1128..1487)
                     /locus_tag="VGA49_00735"
     CDS             complement(1128..1487)
                     /locus_tag="VGA49_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9664325.1"
                     /translation="MPVLTYIFIGFAGGFVGGGLGLGGGAVMVPLLVLAAGLTQHQAQ
                     GTVIGLLTVPVFLAAAWRYYVAGNLRLDITGFMIIGFIAGSFLGAHFVQYLPAAVLKK
                     IFGIALVLIGIKLVLIK"
     gene            complement(1498..>1686)
                     /locus_tag="VGA49_00740"
     CDS             complement(1498..>1686)
                     /locus_tag="VGA49_00740"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX9664326.1"
                     /translation="PMMASVQSFARKHKLRGQAACEEVMACALGACLGCSIKTTRGYK
                     TVCYDGPVFNLDEVIFNH"
BASE COUNT          463 a          443 c          329 g          451 t
ORIGIN      
        1 tcaccgcgtt ggccacagtg ttgggtatat cgtgaaattt caaatcaaga aaaatttttt
       61 tcccggattc atgaataaag tgaactattc gcgttccgca tgccgtaaaa atctgactgc
      121 cgactttaaa aatatccaca tcatccttta aaagattaag taattgacag gcctcatcaa
      181 atgtttcaac atccaaagca acaataagtt gtcctttttc tttcctcatg acttcacaca
      241 cccaatcagt ttatgaatat ctgtaacttt atttttccgc atgtaagata ttatcccgtt
      301 taacacgtcc aacggggccc gcggattgat aaaattcgct gttccgacgg ccaccatggt
      361 tgcgccggca ataatatact gcagggcgtc ttctcctgtc gtaattcccc ccatagcaac
      421 aatgggaatc tttatttttt gggctgtttt ataaaccata tcaactgcca tcggacgtat
      481 ggccggcccg cttacaccgc ccgtaacatt ccccaaaacc gaggcgcgct tgtgaacatc
      541 aatgaccatc ccagaaaaag tattgatcaa actgactccg tccgcgccgg cgtcctccgc
      601 cgctttcgcg atagtggtaa catccgtaac attcggggcc aatttcgcga taacgggata
      661 atcggaaatt tttttgacag ctttaacaac gcggtaagtt gctttgggat cttgcgcgac
      721 cagaactttt tttcctaaat tcgggcagga taaatttaat tctaaaacgc ttacaccgcc
      781 agccttatta aacttctgtg ctaaaacggt aaattcccta tccgtatgcc ccaagatact
      841 gattataatc ggaatgccga ttcccttaaa gagcggcaat tttgcctgaa taaaggcgtc
      901 cgctcccgga ttttcaatgc caatggcgtt gagcatcccc gacggcgttt caacaattct
      961 ggggggcgga ttgccttcct gggctttcaa agtaactgtt ttaggcgtta acgcccctaa
     1021 ttttttgact tcttcgagat tgtaatattt ttcagaatgc ccgaaggttc ccgaggccac
     1081 cgcgacagga tttttaaatt taatttttcc tatggtaaca gcaatgttca ttttattaaa
     1141 accagtttta tcccgatgag aacgagagcg attccaaata tttttttcaa tacggctgcc
     1201 ggcaaatact gcacaaaatg cgcccctaaa aacgatccgg caataaagcc gataatcata
     1261 aaccctgtaa tatccaaccg taaattaccc gccacatagt accgccacgc ggcggctaaa
     1321 aacaccggca ccgttagcag tccgatgacc gttccttggg cttgatgctg tgtcaggccc
     1381 gcggccaata ccaataaagg caccataacc gccccgcctc ccaatcctaa tccgccgccg
     1441 acaaaccctc ctgcaaaccc aatgaatata taagtaagaa cgggcatgag aacctcctta
     1501 atggttgaag atcacttcat ctaaattaaa aaccggcccg tcataacaaa cggttttata
     1561 gccccgcgtt gttttaatcg agcatccaag gcatgctccc aaagcgcatg ccatgacctc
     1621 ttcacacgcg gcctgtcctc ggagtttatg tttccgggcg aaagactgga cggaggccat
     1681 catagg
//
LOCUS       DASWHZ010000031         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3400544, whole genome shotgun sequence.
ACCESSION   DASWHZ010000031 DASWHZ010000000
VERSION     DASWHZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3400544"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1207)
                     /locus_tag="VGA49_00745"
     CDS             complement(194..1207)
                     /locus_tag="VGA49_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9664327.1"
                     /translation="MDQLVINDLIYGQQVITEPVVLELIQSKTFQRLQDIDQGGYAPI
                     CYNPHNISPAELKKNNRFEHSVGVYLLLNKYNDSLESQVSGLLHDVSHPPFSHCVDYV
                     FEEEGSQTDHSFQDNRHADFIKNSEISDILKKYKFDPDQIIHEANFLLLENKIPDICA
                     DRIDYSLRTALLFKEYSNEQTDKMLDDLKAEAGVWYFAQTQSARDFAGLFKKMNDYHY
                     SGLPTAVAFTVVSKVLKYCLRTGYLEKADFDRTDSFVLNKIKANLKKDEILAGLYRDL
                     NNRYSYQQVEKGEYSIFCKSRVIDPYVKQGGKLARLSGIYKDWSQVVTQDLKPKKYYL
                     EKT"
     gene            complement(1301..1447)
                     /locus_tag="VGA49_00750"
     CDS             complement(1301..1447)
                     /locus_tag="VGA49_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664328.1"
                     /translation="MKKKIKHNDLEYNQIDSYIYIGTNACCGTHFEEKLLTKGIRADI
                     GKGD"
     gene            complement(1425..>1781)
                     /locus_tag="VGA49_00755"
     CDS             complement(1425..>1781)
                     /locus_tag="VGA49_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HEX9664329.1"
                     /translation="AKIIKQFIGQLEQVPPVYSAVKVKGKEAYKLARKGQAVALQPRK
                     VEIKAIEIIDYGWPYVKIRVVTGPGVYIRSLARDLGKKLKTGGYLADLERTRVGQFTK
                     KEALSLKKFYEEKNKT"
BASE COUNT          494 a          386 c          321 g          580 t
ORIGIN      
        1 gtctgactcc ggtggcaaac atctttgctc atcccgactt aagccgctgt taaactccgc
       61 caaactcttc aaaaaccgcc cataatcccg atcatctaaa aaaacatgac gtttgtccac
      121 cccgcggttg tagatatgat aaaattcccc gttgtaaaat tttaatctca taattttact
      181 tgggggtgga aatctaggtt ttttctaaat aatatttctt cggctttaaa tcctgggtta
      241 cgacttggga ccagtcttta tatattcccg acagacgggc taatttacca ccttgcttca
      301 cataaggatc aatcacccgt gatttgcaga aaattgaata ttcccctttt tcaacctgct
      361 gatagctgta acgattgttc aaatctctat ataaaccagc taaaatttca tcttttttca
      421 gattagcctt tattttgttt agcacgaaac tatcagtccg gtcaaaatcg gctttttcca
      481 gatagcctgt ccgcaggcaa tatttcaaga ccttgctgac aacagtaaag gcgacagccg
      541 tcggcaggcc ggaataatga taatcattca ttttcttaaa aagaccggca aaatccctgg
      601 ctgactgggt ctgtgcaaaa taccaaacgc cggcttcagc ttttaaatca tctagcattt
      661 tatccgtctg ctcgtttgaa tattctttaa acaacaatgc tgttctcaaa gagtaatcaa
      721 tccggtcagc gcaaatatca ggaattttat tttccagcaa caggaaattg gcttcatgaa
      781 taatttggtc aggatcaaac ttatattttt ttaaaatatc cgaaatttct gaattcttaa
      841 tgaaatcggc gtgccggtta tcctgaaaac tgtggtcagt ctgggaacct tcctcttcaa
      901 aaacataatc aacgcagtgg gaaaaaggtg ggtgagaaac atcatggagt aatccggaaa
      961 cctgagattc caggctgtcg ttatatttat taagcaataa ataaacgccg actgaatgtt
     1021 caaatctgtt atttttcttt aattcagccg gagaaatatt gtgtgggttg taacagatcg
     1081 gggcgtagcc gccctgatca atatcttgta atcgttgaaa agttttggac tgaatcaatt
     1141 ccaaaacaac aggttcagta atcacctgct gtccgtaaat tagatcatta ataaccagct
     1201 gatccattat ttaaactttt ttatataagc cttaaattct ttgagctgct tttcctgcga
     1261 cggttcaatg tgaatcgtcg gtcgtttttg agatattttc tcaatcgcct tttccaatgt
     1321 cagctctgat gcctttggtc agcagctttt cctcaaagtg ggtcccgcaa caggcgttag
     1381 tgccgatata aatgtaagaa tcaatttgat tatattccag atcgttatgt tttatttttt
     1441 tcttcataaa atttttttaa tgataaagct tcttttttgg tgaactggcc gactcttgtc
     1501 cgttccaagt cagccaaata gccgcctgtc ttcagctttt ttccaagatc acgggccaaa
     1561 gaacggatat aaacccccgg gccggtgact actctaattt taacataggg ccagccatag
     1621 tctataattt ctatcgcttt aatctcaact tttctcggct gtaaggcgac ggcttgtcct
     1681 tttcgggcta atttataggc ttctttccct ttaactttaa ccgcgctata aaccggcggg
     1741 acttgttcca gctgaccgat aaactgttta attattttcg c
//
LOCUS       DASWHZ010000032         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3401907, whole genome shotgun sequence.
ACCESSION   DASWHZ010000032 DASWHZ010000000
VERSION     DASWHZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3401907"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..999
                     /locus_tag="VGA49_00760"
     CDS             145..999
                     /locus_tag="VGA49_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9664330.1"
                     /translation="MKKFHTIRQLERKANQIRQDLIKMLVEAGSGHSAGPLDMADVFT
                     ALYFHVLNHNPKKPDWDQRDRLYLSNGHICPVRYVTMAHAGYFPKKELMTLRKLGTRL
                     QGHPSRHDFPPIESSSGPLGQGLSIACGAAYAARMDGQRHFVYCLTSDGEHQEGQTWE
                     AAMFAGNNKLHNLIQIMDRNNIQIDGHTEEIMPLEPLRQKYEAFNWHVIEIDGHNIEE
                     VIDACNMAKAIYEKPTVIIAHTIAGKGVDFMEYDYLWHGKPPKPDEGREALRQLRSLG
                     GKIVGEHE"
     gene            1118..1882
                     /locus_tag="VGA49_00765"
     CDS             1118..1882
                     /locus_tag="VGA49_00765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9664331.1"
                     /translation="MLDTAIKAVKSGGKIISDNYGRITSLKMKGGNWREVVSQIDLDA
                     NDQMLKVIEAAYPDHNIISEESILPKKPSDYTWYVDPLDGTTNYLMQFPFFAVCVGLA
                     FKGEPILGVVYNPILNELFTAEKDQGAELNGQKIEVSNNNNLKLTLANFCHAENEKSV
                     KQIGKIYEIFKMEARDFRRLGSGGLDMAYLAAGRYDVYISNNDRIWDVIPGYVIAKEA
                     GAKITDWQGSDWTPDSAQLLITNQELHKKVLELLTL"
     gene            1921..2904
                     /locus_tag="VGA49_00770"
     CDS             1921..2904
                     /locus_tag="VGA49_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX9664332.1"
                     /translation="MRNPKANLIKNLFSEKIEKIPTRNGYGEGLVEAGKKDKNVVALC
                     GDLTESTRTEYFQKEFPDRFIEVGVAEQNMMGLAAGLALNGKIPFVSTYAVFCPGRNW
                     DQTRVSVCYNNINVKLTGAHSGISVGPDGATHQALEDIAITRVLPNMTVLAPCDYHQT
                     RRATVAAAKYPGPVYLRFARAGTPVFTTARTPFKICQADVYWEGKDVTIIGAGPLVYE
                     ALKAAQELKKDKISARVINCHTIKPIDKETISRAAKETGAIVTIEEHQIHGGLGSAVA
                     EVVVQNQPVPMEIIGVPDRFGESGEPEELLEHYGLVAKNIVTAVKRILKKK"
     gene            3129..>3975
                     /locus_tag="VGA49_00775"
     CDS             3129..>3975
                     /locus_tag="VGA49_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX9664333.1"
                     /translation="MSKATFDLISIGDSTLDTFVKLDEASVLCDFNHDHCWLCLSYAD
                     KIPIERLDHTTGGNSSNMAVGSARLGLKSAFFTVLGDDDVADKILASLKHDKVDCRYV
                     QRVKKTTTNFSVVLNYQTERTILVYHEKRKYQLPKLQSAKWVYYSSMGKGFQSIHKDL
                     LRYIKKTGARVGFNPGTFQLNAGNRVLANILEMTEVLFVNREEAQRLLGNFKKSTIKE
                     LLRGLRHLGPRTPVITDGPKGAYSFDGKTYYYMPIYDIKVVERTGAGDSFSTGFLTAI
                     IKGKSP"
BASE COUNT         1153 a          824 c          976 g         1022 t
ORIGIN      
        1 caagaacgtt aaagaagcaa ttgggcgttt gaagcaggca gttgggaaat aagtcaacag
       61 ccagcagtca acagggatta ctaatcacca gaccctgata actacccggt tgacggttga
      121 ctaatgactg ttgactatat aaatatgaaa aagtttcaca caatcagaca gctggagcgt
      181 aaggccaatc agatcagaca agatctgatc aaaatgctgg ttgaggccgg gtcgggccac
      241 tcggccgggc cgctggacat ggctgatgtt tttacggctt tgtattttca tgttttaaac
      301 cataatccca aaaagccgga ctgggatcag cgggaccgtc tttatttatc caatggccat
      361 atttgcccgg tccgctacgt gaccatggct cacgccggtt attttcccaa aaaagagcta
      421 atgactttaa gaaaactggg gacgcgtctg cagggacacc cttcccgcca tgattttccg
      481 cctattgaat catcgtccgg gccgctgggc caggggctgt caattgcttg cggagccgcc
      541 tacgccgccc ggatggacgg ccagcgacat tttgtttact gcctgaccag cgacggcgag
      601 catcaggaag gccagacctg ggaagcggcg atgtttgccg gcaataacaa gctccataac
      661 ttaatccaga ttatggaccg gaataatatt cagattgacg gccacactga agaaattatg
      721 ccgctggagc ctttgcgcca gaaatatgaa gcttttaact ggcacgtgat tgaaattgac
      781 ggccataata ttgaagaagt gattgacgcc tgtaacatgg ctaaagcaat ttatgaaaaa
      841 ccaacggtga ttatcgccca caccatcgcc ggcaagggcg ttgatttcat ggagtacgat
      901 tatctctggc acggcaaacc gcccaagccg gatgaagggc gggaagcttt gaggcaatta
      961 agaagtttgg gcgggaagat agtcggggag cacgagtaag ttgtcagcca aaggctgatc
     1021 cacctttggt ggataagatt taagttacca ggtgtaagtt tgggagaaaa aaacttaaat
     1081 cttaaatctt taatcttaaa tctttaatct acttgttatg ttggacaccg cgataaaagc
     1141 agttaaatcc ggagggaaga ttatcagcga taactacggc cggattacca gtttaaaaat
     1201 gaaaggcggc aactggcgcg aggtggtttc acagattgat ctggacgcca acgatcagat
     1261 gctgaaagtg attgaggccg cctaccccga ccataatatt atttccgaag aaagtatttt
     1321 gccaaaaaag ccgtctgatt acacctggta tgttgatcct ttggacggga cgacaaatta
     1381 tttgatgcaa ttcccttttt tcgcggtttg cgtcggcctg gcttttaagg gagagccaat
     1441 tttgggcgtt gtttacaacc cgattttaaa tgagctgttc acggctgaaa aagatcaggg
     1501 agctgaactg aacggacaaa aaattgaagt ttccaataat aataatttaa aactgacttt
     1561 ggccaacttt tgccacgctg aaaacgaaaa atccgtgaaa cagattggta aaatctacga
     1621 aatctttaaa atggaagcca gggattttcg gcggctgggg tcaggcggac tggacatggc
     1681 ttatctggcg gccggccgct atgatgttta catttccaac aatgaccgga tctgggatgt
     1741 tatccccggc tatgtcattg ccaaagaagc cggcgctaaa attaccgact ggcagggcag
     1801 cgactggacg ccggacagcg ctcaactatt aataaccaac caggagcttc ataaaaaagt
     1861 tttggaactg ttaactcttt aactctttaa attgttaaat ggttatattg ttaaattgtt
     1921 atgcgaaacc caaaagcaaa tttaattaaa aatttatttt ctgaaaaaat agaaaaaatc
     1981 ccgaccagga acggttatgg ggaagggttg gttgaagccg gcaaaaaaga taaaaatgtc
     2041 gtggcgctct gcggggatct gacggaatca acccggactg aatattttca aaaagaattt
     2101 cccgaccggt ttattgaagt tggggtggcc gagcaaaata tgatggggct ggccgccggg
     2161 ctggccctga acggcaaaat tccttttgtt tcaacctacg ctgttttttg tccgggccgg
     2221 aactgggacc agaccagagt cagtgtttgt tacaataata tcaatgtaaa gctgaccggc
     2281 gctcattcgg gtatttcagt cgggccggac ggagccactc accaggcttt ggaagatatt
     2341 gccattaccc gggtcctgcc taatatgaca gttcttgccc catgtgatta tcaccagacc
     2401 cgacgggcga cggtggccgc ggctaaatat cccggaccgg tttatttaag gtttgcccgg
     2461 gccggcacgc cggtttttac gaccgccagg actcccttta aaatctgcca ggccgatgtt
     2521 tactgggagg gaaaagacgt aacgattatt ggcgccggcc ctctggttta tgaggcgtta
     2581 aaagcggccc aggaattgaa aaaagataaa atttccgccc gggtgattaa ctgccacacc
     2641 atcaagccga ttgacaagga aacaatcagt cgggcggcca aagaaaccgg agcgattgtg
     2701 accattgaag agcatcagat tcacggcggg ctggggagcg ccgtggctga agtggttgtc
     2761 caaaatcagc cggtgccaat ggagatcatt ggcgtgcccg accgctttgg ggaaagcggc
     2821 gaaccggaag aattattgga gcattatggt ttagtggcta agaatatagt aaccgcggtt
     2881 aagagaatcc tcaagaagaa gtaaaattat tggattatag aattacgcat tacgaaataa
     2941 attaagtaaa aaggtaaaag taaaaaataa aaagttcagg taataagttt cagtcagggg
     3001 ctgatccgcc tctggaggaa aaagttattt aaaataaaaa ttttccaaat tttttacttt
     3061 ttacttatta cttttgactt ataacaatcc gtaatccgca attcgtaatt aattaattgt
     3121 ttaattctat gtccaaagct acattcgatt taatttcaat cggcgattcc accctggata
     3181 ccttcgtcaa gctggatgaa gccagcgtcc tgtgtgattt taatcatgat cattgctggc
     3241 tgtgtttaag ttacgctgat aagattccaa ttgaacggct ggatcacacc accggcggca
     3301 attcttccaa catggccgtg gggtcagccc gcttaggttt gaaatcagct tttttcacgg
     3361 ttttaggcga tgatgatgtt gccgataaaa ttttagctag tttaaaacat gacaaagtgg
     3421 actgccgtta tgttcagcgg gttaaaaaga cgactactaa tttttcagtt gttcttaatt
     3481 atcagacaga aagaacgatt ctggtttatc atgaaaaaag aaaatatcaa ttgccaaaac
     3541 tgcaaagcgc taaatgggtt tattattctt caatgggcaa gggatttcag tcgattcata
     3601 aagatttgtt gcgctatatt aaaaagaccg gggccagagt tggttttaat ccgggaactt
     3661 ttcagttaaa cgccggcaat cgggttctgg ccaatatttt ggaaatgacc gaggttttat
     3721 tcgttaaccg ggaagaagct caaagacttt taggcaattt taaaaaatcc accattaaag
     3781 aattgctccg gggtttgagg catttgggac cgcggacccc ggtgattact gacggaccca
     3841 agggggccta ctcttttgac ggcaaaacct attattacat gccaatttat gacattaaag
     3901 ttgttgaacg gaccggagcc ggcgacagtt tctccaccgg atttttaacg gcgatcatca
     3961 aagggaaaag ccctg
//
LOCUS       DASWHZ010000033         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1572272, whole genome shotgun sequence.
ACCESSION   DASWHZ010000033 DASWHZ010000000
VERSION     DASWHZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572272"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VGA49_00780"
     CDS             complement(<1..116)
                     /locus_tag="VGA49_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664334.1"
                     /translation="MAKTKFIFVAGGVMSGVGKGVACASIGKILQGKGYRVSA"
     gene            complement(118..564)
                     /gene="rplI"
                     /locus_tag="VGA49_00785"
     CDS             complement(118..564)
                     /gene="rplI"
                     /locus_tag="VGA49_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9664335.1"
                     /translation="MKIVILKEIKNLGRQGQVKEVSAGYANNFLIPKGLARPATDKVL
                     AEIQKQVTARRKKQVSQLARNKEVIQKINGLTIGLKVKADDRGNLYGSVSPDQIVQRL
                     NKKGLVVDKSKIKLPENIKKLGNYQINLQLDQENKADLRLIVEAEK"
     gene            complement(754..1971)
                     /locus_tag="VGA49_00790"
     CDS             complement(754..1971)
                     /locus_tag="VGA49_00790"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX9664336.1"
                     /translation="MIFNLIDEAIQDIKDGKFVIVVDDEDRENEGDLIIAAEKVTPEA
                     VNLMITKGKGLVCIAMTKDRLDQLDIHPMVSDNTDPMKTAFTISVDARENTTTGISAF
                     DRAWTIRKLIDPLSGPADFNRPGHIFPLRAKEGGVLRRAGHTEAAIDLARIAGLSPAG
                     VICEIIKDDGQMARLPELFEFARANNLKIISIRDLIEYRKRREKLIEKVVEITLPTAF
                     GDFKAITYQSLTDPKPHLALVKGEVTGHENVLVRVHSECLTGDVFSSQRCDCQAQLQQ
                     SMKMINNEGRGVILYMRQEGRGIGLVNKLKAYKLQEQGYDTVEANHKLGFKADLRDYG
                     IGAQILVDLGLTTIRLLTNNPKKVVGLDAYGLKIVERVPIEIEPNGHNNFYLMTKKTK
                     LGHLLSIDEEKNP"
     gene            complement(2125..3084)
                     /locus_tag="VGA49_00795"
     CDS             complement(2125..3084)
                     /locus_tag="VGA49_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX9664337.1"
                     /translation="MFTLNFKKINNKMVLQAGGKGASLGELTKAGIPVPRGFVVTAET
                     FERFLAETDINLEIDAQLNKVHFEDINSVEKASKAIRDLIGDQIMPQDIGQEILKEFS
                     KLKSKYVAVRSSATAEDSQIASWAGELETYLNTAKETLLDNVKNCWSSLFTPRAIFYG
                     CAKGVYQNGQGRCGVSVAVVVQEMVQSEVAGICFSVHPVTEDKNQMIIEAGWGLGEAV
                     VSGQITPDSYVLDKRDFSLLDINISRQEKMIVRSSKRGDLNKWTPVPGSKQNQQKLTG
                     KQTVELVKLCAKIEKHYKFPVDIEWALKKGKFYITQSRPITTL"
     gene            3161..3469
                     /locus_tag="VGA49_00800"
     CDS             3161..3469
                     /locus_tag="VGA49_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664338.1"
                     /translation="MFHLQISHKFGGKQMKLGGLPTVSQETILIHAKKCMNSDAAKAE
                     FIKEMSLKLKKENPHLLDFIVGISKMQPRGGQQEMLIFVVMQIYALLENQLTTDLTTG
                     "
     gene            3717..>3936
                     /locus_tag="VGA49_00805"
     CDS             3717..>3936
                     /locus_tag="VGA49_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664339.1"
                     /translation="MKKITYHPGVQVLYVEDDKNVAKKYTDALIQNNIFVYHVDNLQD
                     VKDVLKKCEVDVILSDGAYPPKKGGVSTR"
BASE COUNT         1105 a          886 c          755 g         1190 t
ORIGIN      
        1 gccgacaccc ggtagccctt gccctgtaat attttgccga tagaggcgca ggccacgccc
       61 ttgccgacgc cggacatcac gccgccggcg acaaatatga attttgtttt tgccatgcta
      121 tttctctgct tctacgatta aacgcaaatc agctttattt tcctgatcca gttgaaggtt
      181 gatttgataa ttgcccagtt tttttatatt ttcaggtaat tttattttac ttttatcaac
      241 taccaggcct tttttattca gtcgctgaac tatttgatcc ggactgactg atccatataa
      301 atttccccgg tcatctgcct taactttcag gccaatcgtc agaccgttaa ttttttggat
      361 gacctcttta ttcctggcaa gctgactgac ctgctttttc cgcctggcgg tgacctgctt
      421 ttgaatttcc gccagcactt tgtcggtcgc cggcctggcc aagccttttg gtattaaaaa
      481 attattagcg tatccagcgc taacttcttt gacctggccc tggcgaccaa ggtttttaat
      541 ttcttttaag atgacgattt tcataattga tgcattataa cataatattt ataaacctta
      601 aaagcctaac attcaaaacc tttggtatgg tcttaaggtt taggtgtggc tgctggtatg
      661 gtctttggtt ttagttttag gatttacccc ctaaacccat accctaaaac tatactggct
      721 gccataccca aaccctacaa ctaaaaccta tgttcaaggg tttttctcct catcaatcga
      781 cagcagatgt cccaactttg ttttctttgt catcaaataa aaattattat gtccgttcgg
      841 ttcaatttca atcggcaccc tttcaacgat ctttaaacca taagcgtcca gcccaaccac
      901 ctttttcgga ttattggtta aaagcctgat ggtggttaag ccaagatcaa ccaaaatttg
      961 agcgcctatg ccgtaatcgc gcagatcggc tttaaagccc agtttatggt tggcctcaac
     1021 cgtgtcatag ccctgttcct gcaacttata cgcttttagt ttgttgacca gaccaattcc
     1081 ccggccttcc tggcgcatat acaaaatcac cccgcgaccc tcgttattaa tcattttcat
     1141 tgactgttga agctgggcct gacaatcgca acgctgtgaa gaaaaaacat caccggttaa
     1201 acattccgaa tgaactctga ctaaaacatt ttcatgtccg gtaacctctc ctttaaccag
     1261 ggctaagtga ggcttggggt cagtcaatga ctgataggta atggccttga aatcgccaaa
     1321 agctgttggc agagtaattt ccaccacctt ttcaatcagc ttttcccgcc gtttacgata
     1381 ttcaatcaaa tctctaatag agattatttt taaattgttg gcccgggcaa attcaaaaag
     1441 ttccggcagt cgggccatct ggccgtcatc tttaataatt tcacaaatta cgccagctgg
     1501 cgacaatccg gcgatccggg ccagatcaat ggcggcttca gtgtgaccgg cccgtcgtaa
     1561 aacgccgcct tccttagccc gcagagggaa aatatgaccc ggccggttaa aatcagccgg
     1621 cccggacaaa ggatcaatca attttctaat tgtccaggcc cggtcaaaag ccgaaatgcc
     1681 ggtggtggtg ttttcccggg cgtcaacaga aatcgtaaaa gcggttttca tcgggtcggt
     1741 gttgtcgctg accatgggat gaatatcaag ctgatccagc cggtctttgg tcatggcgat
     1801 gcaaaccaaa cctttgcctt tggtgatcat tagattaacc gcttcaggcg taactttttc
     1861 agcggcaata atcaagtcgc cttcattctc acggtcttca tcgtcaacaa cgatcacgaa
     1921 tttaccgtct ttaatatctt ggattgcttc atcaattaaa ttaaaaatca taaaatttag
     1981 gcaataggca ctaggcaata ggcactaggg ttggagttat ttgatgggga tcaggcaaaa
     2041 gttaggctaa tctcttatgc ctctaactat tgcctaatcc ctcgtgcctc gccttgtgcc
     2101 ttgtgcctcg tgccttgtgc ctgtttaaag cgtggtaatc ggccgggatt gagtgatata
     2161 aaacttgcct ttcttcaaag cccattctat atctaccggg aatttataat gtttttctat
     2221 tttagcacaa agtttaacta attcaactgt ttgtttgcct gtcagtttct gctgattctg
     2281 ttttgatccg ggcactggag tccatttatt caaatccccc cgcttagagc ttctaacaat
     2341 catcttctcc tgccgggaaa tattaatatc cagcaaacta aaatcccgtt tatccaaaac
     2401 atagctgtcc ggggtgatct gcccggaaac cacggcttct cccaaacccc agccagcctc
     2461 aattatcatc tggtttttat cttcagtcac cggatgaacg ctgaaacaga tgccggccac
     2521 ttcgctttga accatttctt gaaccaccac cgccaccgat acgccgcaac ggccctggcc
     2581 attttgataa acgccttttg cacagccgta gaaaatcgcc cgcggcgtga acaatgatga
     2641 ccagcagttt ttaacattgt ccagcaatgt ttctttggca gtgtttaaat atgtttccag
     2701 ttccccggcc caggaagcaa tctggctgtc ttcagccgtg gctgaactgc gaacagcgac
     2761 atattttgat ttcagttttg aaaattcttt taaaatttcc tgaccaatat cctggggcat
     2821 tatctgatcg ccaattaaat cccggatagc tttggaagct ttttcaactg aattaatgtc
     2881 ttcaaaatga actttattga gctgggcgtc aatttccaga ttaatatcag tctccgccaa
     2941 aaacctttca aaagtttcag ccgtcaccac aaaaccgcgc ggcaccggaa tgcccgcttt
     3001 ggtcagctca cccaaagaag cgcctttccc gccagcctga aggaccattt tattgttaat
     3061 ttttttgaaa tttaaagtaa acataaatta ttatattata tcaggattaa attattctag
     3121 ccaaaagttt gacattttgg taggaaaatg atatattgat atgtttcact tacaaatctc
     3181 ccacaaattt ggaggtaaac aaatgaaact aggtggtctt ccaactgttt ctcaagaaac
     3241 tatcctaatt catgcaaaga aatgcatgaa ttcagatgcg gccaaagctg aatttatcaa
     3301 agaaatgtct ttgaaactga aaaaggagaa tccgcatctt ttggatttca tagtgggaat
     3361 atcaaaaatg caaccacgag gaggacaaca agaaatgctt atatttgttg tcatgcagat
     3421 ttacgcactt ttggaaaatc agctgacaac agatctaacc acagggtaag caggggtgga
     3481 atctgacact tataaaaccc acaggggctc atcaaaaact gagtcccttt tattttatct
     3541 atctttaaaa gttttttcta tgtaattgca cttcagatta taatttaaaa ctcaaaaaaa
     3601 ttgacatttt ggagggaaaa ggataaaaac gctcaaaaac ctgcaaaaga tgaaaatagt
     3661 gtataattta atgagagttt agatgttttt aaaataaata taaataattg aaaaatatga
     3721 aaaaaattac atatcaccca ggagtccagg tgctatatgt tgaggacgat aagaatgtcg
     3781 ccaaaaaata tacagatgca ttaattcaga ataatatatt tgtttaccat gttgataatc
     3841 ttcaggatgt taaggatgtg cttaaaaaat gtgaggtaga tgtgatttta agtgatgggg
     3901 cttatccacc taaaaaggga ggtgttagta ctaggg
//
LOCUS       DASWHZ010000034         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_459012, whole genome shotgun sequence.
ACCESSION   DASWHZ010000034 DASWHZ010000000
VERSION     DASWHZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459012"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /gene="gatC"
                     /locus_tag="VGA49_00810"
     CDS             complement(<1..254)
                     /gene="gatC"
                     /locus_tag="VGA49_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9664340.1"
                     /translation="MKLTKEQVEYIAKLARIKLTSKEVAKFQDQLSQILNYIEQLGEV
                     DTTDVEPTAQVTGLENVTREDKVEQCDPEVVEKLIEAFPE"
     gene            complement(263..2056)
                     /locus_tag="VGA49_00815"
     CDS             complement(263..2056)
                     /locus_tag="VGA49_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9664341.1"
                     /translation="MNREMAIKDNPIIFLSRKTWEFSRGNRSAVFLYVFLFILGNSVW
                     LAEPLVIAKILNIVQEKGITQDSLPLIFLYLFLALLLPFGFWVFHGPARVIETRNAFL
                     VKANYKKYLLEGTLALPAEWHADHHSGDTIDKIEKSSEALFEYSSDTFQVIETVINLT
                     VAYIALTYFNPHSGYIVLALIVLTMFSITKFDQVLVAQYAKLFKIENKISAKIFDTIS
                     NIVTVISLRIEKMLTNAIVRQIMSPFKLFVSNRKMIEWKWFFVSVGGAVMAFLVIGSY
                     IYFNVKEGAVVLVGTVYVLYGYVQRIQYIFFEFAHRYNTIVRQKTAVLNVGEITQEFK
                     EVDKIGQIRLGKDWSELKIEDLKFSYQTDEGGRQHLDNISLAIKRHEKIALIGASGSG
                     KTTLLKIIRDLYHPQKIKLYLDGKALKEGFRMISQEIALIPQDPEIFSTTIEENITFG
                     IKHKFSLLKKFTDQARFTNVVQKLPKKFQSSVFEKGVNLSSGEKQRLALARGLLASQD
                     KEIILMDEPTSSVDLKNELEIFQNIFREFKDKTIISSIHRLHLLKMFDHIYFFRDGKI
                     IASGTFEELLKSSQEFEKIWEKYTKTKKFMV"
     gene            complement(2061..>2412)
                     /locus_tag="VGA49_00820"
     CDS             complement(2061..>2412)
                     /locus_tag="VGA49_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEX9664342.1"
                     /translation="IGAVVAQSIYDYFQDKGNLKLIDKLRQNGVKILAKKVSRKQTLA
                     GLTFVITGSLESLTRDEAKEKIRQLGGDISGSVSKKTDYLVVGEDPGSKHDKAKKLEI
                     KILDEKEFSRLLIK"
BASE COUNT          739 a          508 c          387 g          778 t
ORIGIN      
        1 ttttccggaa aggcttcaat gagtttttct acaacttccg gatcgcattg ctcaacttta
       61 tcctcccgcg tgacattttc caggccggtc acctgggccg ttggctcaac atcagtcgtg
      121 tcaacttcac ccagctgttc aatataattt aaaatctggg acagctgatc ctgaaatttg
      181 gcgacttctt tggaagtcag tttaatccgg gccagtttgg caatgtattc aacttgttct
      241 ttggtcagtt tcatatgcaa aattatacca taaatttctt ggttttagta tatttctccc
      301 aaattttttc gaattcctga gaggatttta acaactcctc aaaagttcct gaagcaataa
      361 tttttccatc tctaaaaaaa taaatatgat caaacatttt cagcaaatga agacgatgaa
      421 ttgatgaaat aattgtttta tctttaaatt cccgaaatat attttgaaaa atctccaact
      481 cgttttttaa atcaacgctg ctggttggtt catccatcaa aataatttcc ttatcctggc
      541 tggccagcaa tccccgggcc agtgctaagc gctgtttttc accgctgctt aagttaactc
      601 ctttttcaaa tactgacgac tgaaacttct taggaagttt ttgaaccaca ttagtaaaac
      661 gggcctggtc tgtaaatttt ttgagcaatg aaaatttgtg tttaatccca aaagtaatat
      721 tttcttcaat agtagttgaa aatatttctg gatcctgtgg aatcagagct atctcctgac
      781 tgatcattct aaagccttct ttaagcgctt taccgtccag atataatttt attttttgag
      841 gatgatataa atctctgatt atttttaata aagttgtctt gccgctgccg ctggcgccga
      901 ttaaagcaat tttttcatgc cttttaatag ctaaagaaat attatcaaga tgctgccggc
      961 cgccctcatc tgtttgataa gaaaatttaa gatcttcaat ttttaattca ctccagtctt
     1021 tccccagcct aatttgtcca attttatcaa cctctttaaa ttcctgggtt atttccccaa
     1081 cattcagaac tgctgtcttt tgcctgacaa tagtattgta acgatgagca aattcgaaaa
     1141 atatatactg gattctctga acataaccat aaaggacata aactgttcca actaaaacca
     1201 ctgccccctc tttgacatta aaataaatat aggaaccaat gaccagaaaa gccatcactg
     1261 ccccgccgac cgacacaaaa aaccatttcc actcaatcat tttccggttg ctgacaaata
     1321 atttaaaagg actcattatt tgcctgacga tggcattggt tagcatcttt tctattctca
     1381 aagaaataac cgtaacaatg ttactaatag tatcaaatat tttagcggaa atcttatttt
     1441 ctattttaaa cagtttggca tattgagcga ctaaaacttg atcaaatttg gtgatgctga
     1501 acattgttaa aacaatcagc gccaaaacaa tatagcctga atgcgggttg aaatatgtta
     1561 aagcaatata agcaaccgtt aaattaataa cagtttcaat aacttgaaat gtatcagatg
     1621 aatattcaaa taaagcttca gaactttttt cgattttatc aatggtatcg ccggaatgat
     1681 ggtcagcgtg ccactcggcc ggcaaagcca aagttccttc cagtaaatat tttttatagt
     1741 tggccttgac taaaaaagcg ttcctggttt caattacccg ggccggacca tgaaaaaccc
     1801 aaaaaccaaa aggcaataat aaggctaaaa acaaatataa aaaaattaaa ggcagactat
     1861 cctgggtaat ccccttttct tggacaatat taaggatctt ggcaatgacc agcggttcag
     1921 ccagccaaac actgttgccc aaaataaata aaaaaacata cagaaaaact gccgatctgt
     1981 tgcccctgga aaactcccag gtttttctgg ataaaaaaat aattggatta tcttttatag
     2041 ccatttcacg attcaaaatt ttattttatt agtaaacgcg aaaactcctt ctcatccaaa
     2101 atcttaatct ccagcttttt ggctttgtca tgttttgagc cgggatcttc gccgaccacg
     2161 agataatcag tctttttgct gactgaaccg ctgatatcgc cgcccagctg tctgattttt
     2221 tctttggctt catctctggt taaactttcc aggctgccgg tgatcacaaa agtcagtccg
     2281 gccaaagtct gcttccggct gactttcttt gccaaaattt tgactccgtt ctgacgaagc
     2341 ttatcaatta atttcaaatt gcctttatcc tgaaaataat catagatgct ttgagcgacg
     2401 actgcgccaa ta
//
LOCUS       DASWHZ010000035         4509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1191334, whole genome shotgun sequence.
ACCESSION   DASWHZ010000035 DASWHZ010000000
VERSION     DASWHZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191334"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /gene="mnmA"
                     /locus_tag="VGA49_00825"
     CDS             <1..1030
                     /gene="mnmA"
                     /locus_tag="VGA49_00825"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9664343.1"
                     /translation="AAALLQEQGYDVMGVSMLLWGDESFYGKEVVLNSKCCSVDEASL
                     VAHQLGFTFQTLDFKNEFKEIVVDDFLKDYQSGKTPNPCVRCNKHIKFGALLDKAREL
                     GADYVATGHYVRIKRTADGLVKIFQGRDKSKDQSYFLCDLSQDQLKQIVFPLGDYHKH
                     EVRRLAKEFGLRIHNKAESQEICFVPYKKHNLFLKRHLDLKKGPIKNLAGDVLSEHDG
                     LPLYTLGQRKGIKIGGAGPYYVIGLDYESNTLYVSNDKQDPKLYSDQLIARNVNWISG
                     QAPVAPLQITCSIRYGNYAEGVIEKKINETDYLVKFNNPVRAVTPGQSAVFYDGEELI
                     GGGVIDRV"
     gene            1041..2828
                     /locus_tag="VGA49_00830"
     CDS             1041..2828
                     /locus_tag="VGA49_00830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9664344.1"
                     /translation="MTAGVIKQKFLEFFKEKGHAIIPSASLIPEHDPTVLFTTAGMHP
                     LVPFLMGEKHPAGRRLADVQKSLRTGDIDEVGDAWHLTFFEMLGNWSLGDYFKEEAIS
                     WAYEFLTDKKWLGIDPANLYFSVFEGDEDAPRDEEAARAWEALGVLDDQIYYFPKSEN
                     WWGPAGETGPCGPDTEIFYDTGREKCGPDCNPSCDCGKYTEIWNIVFMQYLKTKEGRY
                     LPAEQKNIDTGMGLERTAAVLQGKDNAYETELFRPIIDKILDLTGQKSVDRSELILSH
                     QKPIRIIADHLRAAVFILADGVVPSNLDQGYVLRRLIRRAIRHAKMIGIPVEKNLTVP
                     IAEVVIDVFAKDYRELSVNKDKLIRELRAEEEKFEKTLELGLQEFNKLADSSKKEING
                     RDAFKLFSTYGFPIEITKELALEKKLKVDQAGFDQEFEKHQELSRVGAEKKFKGGLAD
                     ASGETTKLHTATHLLDQALRDVLGDHVEQRGSNITPKRLRFDFTHPKKMTDLEIKKVE
                     AIVNQKIKEDLPVSFQEMTVKEARELGAIGLFEERYGDRVKVYIIGDYSREICGGPHV
                     TKTGLLGNFKITREESSSAGIRRIKAILD"
     gene            3002..3214
                     /gene="infA"
                     /locus_tag="VGA49_00835"
     CDS             3002..3214
                     /gene="infA"
                     /locus_tag="VGA49_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9664345.1"
                     /translation="MKKDLIEIDGAVIEALPSATFKIKLENGHEILGYLSGKMRMYRI
                     KILPGDRVKVEMTPYDLTKGRIVYRY"
     gene            3224..3340
                     /gene="rpmJ"
                     /locus_tag="VGA49_00840"
     CDS             3224..3340
                     /gene="rpmJ"
                     /locus_tag="VGA49_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX9664346.1"
                     /translation="MKVRSSVKPICKDCKVVRRKKRLVVICPKNPKHKQRQG"
     gene            3347..3733
                     /gene="rpsM"
                     /locus_tag="VGA49_00845"
     CDS             3347..3733
                     /gene="rpsM"
                     /locus_tag="VGA49_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9664347.1"
                     /translation="MARISGVTIPDNKRIEVALTYIFGIGRSQSREILKSVDVDFNIR
                     TKDLSEDQLNKIRDAAAKLPIEGDLRREILLNIRRLKEIGSYRGSRHTRRLPVRGQRT
                     KTNSRSIRGNVRRTMGSGRKPTAQKT"
     gene            3807..4271
                     /gene="rpsK"
                     /locus_tag="VGA49_00850"
     CDS             3807..4271
                     /gene="rpsK"
                     /locus_tag="VGA49_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9664348.1"
                     /translation="MDQEKNTTITDETAVQPEAAQAKEKPVRKIRSRKKKQTQIPVRG
                     TAYILSTYNNTVVTITDQNGNALAWSSAGKVGFKGPKKATAYAAGVIVKDVVPKLIDQ
                     GLREVDVFIKGVGTGRESAIRSLNSNGLKIISIKDVTPIPHNGCRPKKVRRV"
     gene            4283..>4509
                     /locus_tag="VGA49_00855"
     CDS             4283..>4509
                     /locus_tag="VGA49_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008108097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9664349.1"
                     /translation="MGRNRQPQCKQCRREGEKICYKHKCATIKRNYAPGVHGVKRQGR
                     LSEYGQQLRAKQKVKRLYGLMEKQFSNYYKKS"
BASE COUNT         1376 a          879 c         1089 g         1165 t
ORIGIN      
        1 cgccgccgct ctgcttcagg aacagggcta tgatgtcatg ggtgtctcca tgctactctg
       61 gggcgatgaa agtttttacg gcaaagaagt tgttttaaac agcaaatgtt gttctgttga
      121 cgaggccagc ctggtggccc atcagcttgg ttttactttt cagaccctgg attttaaaaa
      181 tgaatttaag gagatagtcg ttgatgattt tttgaaagat tatcagtcag gcaaaactcc
      241 caatccctgc gtccgctgca ataaacatat taaattcggc gctctcctgg ataaagccag
      301 ggagttggga gccgattatg ttgccaccgg tcattatgtc agaattaagc ggacggccga
      361 cggtttagtg aaaatttttc agggcaggga taaatcaaaa gatcagtctt attttttatg
      421 cgaccttagt caggaccagc tgaaacagat cgtttttccc ctgggagatt atcataaaca
      481 tgaagtcagg cggttagcca aagaatttgg tttgcggatc cataacaaag ccgagagcca
      541 ggaaatttgt tttgtgcctt ataaaaaaca taatttgttt ttgaagcggc accttgatct
      601 taaaaagggg ccaattaaaa atcttgccgg agatgttttg agcgaacatg acggtctgcc
      661 gctttatact ttgggtcaga gaaagggcat taaaattggc ggcgccgggc cttattatgt
      721 gattggtttg gattatgaat ccaatacgct ttatgtttcc aatgataagc aggatccaaa
      781 actttacagc gaccagctga ttgccagaaa tgtcaactgg atttcaggcc aggcgcccgt
      841 ggcgccgctt caaataacct gcagtattcg ctacggcaac tacgccgaag gggtgattga
      901 aaaaaagatt aatgaaaccg attatctggt aaagtttaat aacccggtaa gagcggttac
      961 tcccggccag tcagcggttt tttatgatgg cgaagagtta attggcgggg gagtgataga
     1021 tagggtataa ttcaaaaaat atgacagccg gtgtaattaa acaaaaattt ttggaatttt
     1081 ttaaagagaa gggacacgcg attattccct cggcttcttt gattcctgaa catgatccaa
     1141 cggttttgtt tacaaccgcc ggcatgcatc ctttggtgcc ttttttaatg ggggagaagc
     1201 atccggccgg ccgaagactg gctgatgttc aaaaaagtct ccggacaggc gatattgacg
     1261 aggtcggcga cgcctggcat ctgacttttt ttgaaatgct gggcaactgg tccctggggg
     1321 attattttaa ggaagaagcc atcagctggg cttatgaatt tttaactgat aaaaaatggc
     1381 tggggattga tccggctaat ctttattttt ctgtttttga gggtgatgaa gacgcgcccc
     1441 gggacgaaga ggccgcccgg gcctgggagg ctttgggagt tttagatgac caaatttatt
     1501 attttcccaa atctgaaaac tggtgggggc cggccggcga gactggcccc tgcgggccgg
     1561 acactgaaat tttttatgat accgggcgcg aaaaatgcgg tccggactgt aatccctcct
     1621 gcgactgcgg caaatacacg gaaatctgga atattgtttt tatgcagtat ttaaaaacta
     1681 aagaaggccg gtatctgccg gcagaacaaa aaaacattga taccgggatg gggctggaaa
     1741 gaacggcggc tgttctgcaa ggcaaagata acgcctatga aaccgagctc ttccggccga
     1801 ttattgataa aattctcgat ttaactggcc agaaatcagt tgaccgttct gaattaatct
     1861 taagtcatca aaaacccatc agaattattg ccgaccatct cagagccgct gtttttatac
     1921 tggccgacgg agtggttccg tcaaatcttg atcagggtta tgttttaaga cgtttgattc
     1981 gccgggcaat cagacacgcc aaaatgattg gtattccggt ggaaaaaaat ttaacagtcc
     2041 cgattgccga agtagtgatt gatgtttttg ccaaagatta ccgggagcta tccgtgaaca
     2101 aagataaact tatcagggag ttaagggctg aagaagaaaa atttgaaaaa acactggagc
     2161 ttggcctgca agagtttaat aaacttgccg acagctcaaa aaaagaaatc aacggtcggg
     2221 acgcgtttaa attattttca acatatggtt ttccaattga gattaccaaa gagctggccc
     2281 tggaaaaaaa attaaaagtt gaccaggctg gctttgatca ggaatttgaa aaacatcagg
     2341 agttatcccg ggttggcgct gagaagaaat ttaaaggcgg cctggctgac gcttccgggg
     2401 agacaaccaa acttcatacc gccactcatt tactggatca ggcgttgcgt gatgtcctgg
     2461 gcgatcatgt tgaacagcgg ggcagcaaca ttacgccaaa aagactaaga tttgatttta
     2521 cccatcctaa aaaaatgacc gatttagaaa ttaaaaaagt tgaagcaatt gtcaatcaaa
     2581 aaattaaaga agatctgccg gtttcatttc aggagatgac tgttaaggaa gccagggagc
     2641 tgggagcaat aggtttgttt gaagaaagat acggcgaccg ggttaaagtc tatataatag
     2701 gcgactattc cagagagatt tgcggcggtc cgcatgttac taagaccggc cttttgggaa
     2761 atttcaaaat taccagagaa gagtcatctt cagctggtat tcggcgaatt aaggcaattt
     2821 tggattgatt ttggctaata ttaactaaaa ttagtgggga taaactgttt attttgattt
     2881 ttggctgatt tcttgacata tttttgattt actgtataat ttaataaatg aaaattttat
     2941 aagatattta atttttctat aaattcaggt attttttata tctggaattt tatttatttt
     3001 aatgaagaaa gatttaattg aaattgacgg cgcggttatt gaagctttgc cgtcagccac
     3061 ctttaagatt aagctggaga atggccatga aattctgggc tacctgtcag gcaagatgag
     3121 gatgtacagg ataaaaattt taccaggaga cagagtgaag gtagagatga cgccttatga
     3181 cctgaccaaa ggccggatag tttatcggta ttaatttaaa tcaatgaaag tcagatcgtc
     3241 agttaagcca atttgtaaag actgcaaagt agtcagacgg aaaaaacgtc tggtggttat
     3301 ttgtcccaaa aatccaaaac ataaacaacg acaaggataa aaaattatgg ccagaatatc
     3361 gggagtgaca attccggaca acaaaagaat cgaggtcgcc ctgacctaca tttttggaat
     3421 tggccggtcg cagtccaggg aaattctaaa atcagtcgat gttgatttta acatcagaac
     3481 caaagattta tcagaagatc agctgaacaa aatcagagac gcggccgcta aactgccgat
     3541 tgaaggcgat ttgcgccggg aaattttact gaatatcaga cggctgaaag aaattggctc
     3601 ataccgtggc tcgcgtcaca ccaggcggct gccggtcaga ggccagcgga ccaaaaccaa
     3661 ttcccggtca atccgcggca atgtccggcg gaccatgggc agcggccgaa agccgacggc
     3721 ccagaagact tagcaagtta gtatctggta gttggtaact ggtaagaacc acaacaacca
     3781 actaccaaca accaactact aaatttatgg atcaggaaaa aaacacaacg attacagacg
     3841 aaaccgctgt tcagcctgaa gccgcccagg ctaaggaaaa gcctgtccgg aaaatcagaa
     3901 gccgcaagaa aaaacaaact caaatccctg tccggggcac tgcttatatt ttatcaactt
     3961 ataataatac tgtcgtgacg attactgatc agaacggcaa cgccctggcc tggtcgtcag
     4021 ccggcaaggt tggctttaaa ggtcccaaaa aagccactgc ttacgcggcc ggggtgattg
     4081 ttaaggatgt tgttcccaaa ctaattgatc aggggctgag ggaagttgat gtttttatca
     4141 aaggcgttgg tactggccgg gaatcagcca tccggtctct taattccaat ggtttaaaaa
     4201 ttatatcaat taaggacgtc actcccattc cccacaatgg ctgccgtcca aaaaaagtca
     4261 gacgagttta aatattaaaa ctatgggcag aaacagacaa ccacaatgta aacaatgccg
     4321 gcgcgagggt gaaaaaatct gttacaagca caagtgcgcc actattaaaa ggaactacgc
     4381 tcccggagtt catggtgtca agcggcaggg acggctgtca gaatatggcc agcaattaag
     4441 agccaagcag aaagtcaagc ggctttacgg tctgatggaa aagcagttca gcaattatta
     4501 taaaaaatc
//
LOCUS       DASWHZ010000036         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1193289, whole genome shotgun sequence.
ACCESSION   DASWHZ010000036 DASWHZ010000000
VERSION     DASWHZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193289"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGA49_00860"
     CDS             <1..202
                     /locus_tag="VGA49_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9664350.1"
                     /translation="PEFVSQFEKEIGAAKDAEIIVYCSSATCQASVQASNALEEAGYT
                     NVAHYQDGLAGWQEAGYEFESQ"
     gene            373..699
                     /locus_tag="VGA49_00865"
     CDS             373..699
                     /locus_tag="VGA49_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664351.1"
                     /translation="MSLTKEIKDLLIKKFAGHQNIPRGLFELATYFRHNGSISFDYKE
                     HEDVVIAVSNNFRYGSIVAQGKDIKELEKNIPDAILTSFDVPSSYAREAKIYKVDQMK
                     REYAIA"
     gene            1041..1271
                     /locus_tag="VGA49_00870"
     CDS             1041..1271
                     /locus_tag="VGA49_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664352.1"
                     /translation="MEKFVSLKNFRLKLTDYAKRIEKKGESFLVLKRSKAVFKVVPVE
                     EENWETIIDFTQIDPKGVPFYEQRLKSPDKTQ"
     gene            1396..1626
                     /locus_tag="VGA49_00875"
     CDS             1396..1626
                     /locus_tag="VGA49_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664353.1"
                     /translation="MKNHKRTRFLEFLLIGVVMGVIEDILAIIVVTDSKITLDAIPII
                     LIIAIPFAFLSEYVVDHPDFWTKLLRINKVKK"
     gene            1827..3248
                     /gene="glnA"
                     /locus_tag="VGA49_00880"
     CDS             1827..3248
                     /gene="glnA"
                     /locus_tag="VGA49_00880"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9664354.1"
                     /translation="MTYPKNVKDFLSLIKYEDIKFIDVKLCDAFGQWHHLTYPAARFT
                     TEYFSRGIPFDGSSMPWWRDIKESDMILIPDPTTAFVDPFFEEKTLSVICDAAHPGSK
                     RGYVRDARVIARKALKVLKKTKVADMAYMGSEVEFFIFDKLTFINTPNQVGYEIDSTE
                     GWWNTTKSKSEGRAGNEIPYWEGCYPVSPLDKTGQLRLEMAETLEKIGLTVEKAHHEG
                     GTAGQSEIDFRYDNLLKSADNVMKYQYVLKNVGQLHQKHVSFMPKPMANECGSGMHCH
                     FSLWKKGKNLFAGRGASGISRTALYAIGGILEHGRALTAFTNPSTNSYHRLIPGYHAP
                     VYLTYSAKNRSAAIRIPSEDQPEARRIELRFPDPSANPYLAFTAILMAAIDGIKRKIS
                     PGRPFDRDTYRLTAEEKRRYTALPKSLSGALHALRDDSKFLQRGNVFSKDFLNLWIME
                     KEEEIETIQKIPHPKEFELYYYL"
     gene            complement(3270..3833)
                     /locus_tag="VGA49_00885"
     CDS             complement(3270..3833)
                     /locus_tag="VGA49_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664355.1"
                     /translation="MVFSIINRLVEGVKGGVKASLEGVADVSGAVVDAVKKTVVTTVK
                     ESSEFLADGVSAAASIVQGAVGGVADIGATVVQSIKGIVRGTVEGLMKEGVKQDEAVK
                     GAVGQALHSAKELGFNIGDTAVEAVKGSVNAVKEIGGDVAGATKSSIKAALDAAKEIG
                     GETYEAVKGSLKSGVEGADEIIESLKK"
     gene            3923..4450
                     /locus_tag="VGA49_00890"
     CDS             3923..4450
                     /locus_tag="VGA49_00890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9664356.1"
                     /translation="MSQNLNWPNTLSIIRLILAVVFLVFLLQFKTSQALIIFIIALMT
                     DWADGYIARKKGQVTILGKILDPLADKLLFIIAFVSLSLNYALPVWVAWIVVGCYIIL
                     LSGGVVFVKKGISILKHSWLGRGAFALQAVVVAGVLLSYSGQAWLTIIIYLMVFLTAS
                     TCYYYLAQGFKLLKK"
BASE COUNT         1342 a          880 c          939 g         1323 t
ORIGIN      
        1 ccctgaattc gtcagtcaat ttgaaaaaga gattggcgcg gccaaagacg ccgaaatcat
       61 cgtttattgt tcatcagcga cttgtcaggc cagcgttcag gcctcaaacg ccctggagga
      121 agctggttat actaatgtcg ctcattatca agacggtctg gccggctggc aggaagcggg
      181 ctatgaattt gaaagccaat agaatttatg taaaattaga tgaataaaaa gtttattgaa
      241 acagtggatg cccgggaaaa attctgaatt atttgaataa ttatttgaga taattggcct
      301 acttttttaa gatgtgttat agttgacata taagctaata tatgatatga agttgatgta
      361 agaaaaatat ctatgtcatt aaccaaggaa attaaggatt tattaataaa gaaatttgcc
      421 ggacatcaaa atatcccccg gggtttgttt gaattagcca catatttcag acataacggc
      481 agtatcagct tcgactataa agagcatgaa gacgtggtta tcgctgtcag taataatttt
      541 agatatggca gtattgtcgc tcagggaaaa gacattaaag agttggaaaa aaatattcct
      601 gatgctattt taacttcttt tgatgttcct tcttcatatg ccagggaagc caagatatat
      661 aaggttgatc aaatgaaaag ggaatatgcc atcgcttagt gaattgcctg gtgaattaaa
      721 aagaaaaaaa tttattaagg cgttaaaccg ccttggtttt attatagata caaaaggagg
      781 cgacggcagt cacttcaagg taacctggcc gcaatcacaa aaaagcataa caattccttc
      841 caggctgcct aagcaagttt tatcgtatgt tttaaaagag atcgagaagc attcacaaaa
      901 aagctgggaa gatataaaaa aatatttaag atagtctatg ccctccatcg acaaatttgg
      961 ctggatttat ataggaggat ttgacaaacg actagctaaa gtttaaactt aagatataat
     1021 ttataattat aatactttct atggaaaaat tcgtttcatt aaaaaatttt cgcttaaaac
     1081 tgactgatta cgccaaacgg attgaaaaaa agggagaaag ctttttggtt ttaaaaagat
     1141 ctaaagctgt ttttaaagtt gtgccggttg aagaagaaaa ctgggaaaca atcattgatt
     1201 ttactcaaat tgatcctaag ggagttcctt tttatgaaca gcgccttaag agcccggata
     1261 aaacacagta agaaaaaaac caagaccggc aaaaaaatta aagaaaagac aaaaaaaaga
     1321 aagtagtaat tttttaattc aaaaaaaatc atgcctgaat ccgggcatga tgtttttatg
     1381 ctattattag ataatatgaa aaaccataaa agaaccagat ttttggaatt tttattgatc
     1441 ggcgtggtga tgggagtgat tgaagatatt ttggcgatca ttgttgtgac tgattccaaa
     1501 atcactctgg acgctattcc aattattctg atcattgcta ttcctttcgc ttttttgagc
     1561 gagtacgtgg ttgaccaccc ggatttttgg accaagcttt tgagaataaa taaagtaaaa
     1621 aaataacccc accgtggtga atggcagggt aggggtaagg ccaggctcta gtgacttagt
     1681 tccttctttg aatagttttg taaaatttat gcttttatgt tatgatgata ttgagatatc
     1741 tatgatatcc aatctaccaa atattaatac ttattaattt ttacagggaa atcctttaaa
     1801 tcacgcgagg gtttttttta ataattatga cttacccaaa aaacgtcaaa gatttcctgt
     1861 cgctgattaa atatgaagat attaaattta ttgatgttaa attgtgcgac gcgtttggcc
     1921 agtggcacca tttaacctat ccggccgcca gatttacgac tgaatatttt tcgcggggca
     1981 ttccctttga cggttcttca atgccctggt ggcgcgacat taaagagtca gacatgattt
     2041 taatccctga cccgacgacc gcttttgtcg atccgttttt tgaggaaaaa actttgtcgg
     2101 ttatttgcga cgccgctcat cccggatcca aaaggggtta tgtccgcgac gcccgggtga
     2161 ttgccagaaa agctttaaag gttttaaaaa agaccaaagt cgctgacatg gcttacatgg
     2221 gatcggaagt tgaatttttt atttttgaca aactgacctt tatcaacacg cccaaccagg
     2281 ttggttatga aattgattct accgagggtt ggtggaacac caccaaaagc aagagcgagg
     2341 gccgggccgg caatgaaatc ccttactggg aaggctgtta tccggtttcg ccgttggata
     2401 aaaccggcca gctccggttg gaaatggctg aaactttgga aaaaattggc ctaacggttg
     2461 aaaaagccca tcacgaaggc gggacagccg gacaaagtga aattgatttt cgttatgata
     2521 atttgctgaa atccgccgat aatgtcatga aatatcagta tgttttgaaa aatgtcggcc
     2581 agcttcatca aaagcatgtg tcgtttatgc ccaagccgat ggccaatgag tgcggcagcg
     2641 gcatgcactg ccatttttca ttatggaaaa aaggcaaaaa tttatttgcc ggccgcggcg
     2701 ccagcggaat atccagaacc gctttatacg ccattggcgg gattttggaa cacggccggg
     2761 ccctgaccgc ttttaccaat cccagcacca attcttatca ccggctgatt cccggatacc
     2821 acgccccggt ttatttaact tattccgcta agaaccgctc ggcggccatc agaataccga
     2881 gtgaagacca gcccgaagcc cgccgaatcg agctccggtt tcccgaccca tccgccaatc
     2941 cttatttggc tttcacggcg atattgatgg cggcgattga tggcatcaaa agaaaaattt
     3001 ctcccggccg gccgtttgac cgggatactt accgattaac agccgaggag aaaagaagat
     3061 acactgcttt gcccaaatct ttaagcggcg cgctgcatgc tttgcgggat gacagcaaat
     3121 ttttgcagcg cggcaatgtt ttcagcaaag atttcctaaa tctctggatc atggagaaag
     3181 aggaagaaat tgagactatt cagaaaattc cccatcctaa agaatttgag ctgtattact
     3241 atttataatg acttcaaatg gctgataaat tattttttaa gggattcaat aatttcatca
     3301 gcgccttcaa ctccgctttt taacgatcct ttgacggctt cataagtttc gccgccaatt
     3361 tccttagcgg cgtctaaagc ggctttgatt gaagatttag tggcgccggc gacatcaccg
     3421 ccgatttctt taacggcatt gactgaccct ttgacagctt caactgctgt atcaccaata
     3481 ttgaagccaa gttctttggc gctgtgcagg gcctggccaa cagctccttt aaccgcctcg
     3541 tcctgcttaa ctccttcttt catcaaacct tcaactgttc cccggacaat tcctttaatg
     3601 ctttggacca cagtcgctcc aatatcagcc acgccaccga cagctccctg gacgattgaa
     3661 gcggcggcgc tgacgccatc agctaaaaat tcactgctct ctttgacagt cgtaacgacc
     3721 gtttttttaa ccgcgtcaac aacggctccg ctgacatcgg cgaccccttc caggctggct
     3781 tttacgccac ccttgacgcc ctctaccagg cgattgatta ttgaaaaaac catatttttt
     3841 gattaattat taattcttat actatagcat attaaaaaaa tttaattgtg gataaaatgt
     3901 ataaaggtgt tataataaat taatgtctca aaatttgaac tggcccaata ctttaagcat
     3961 tatcaggctt attttagcgg ttgtcttttt agttttttta ctgcaattta aaacaagtca
     4021 agctttaata atttttatta tcgccctgat gactgattgg gctgacggct atatcgcccg
     4081 caaaaaaggc caggtgacaa tcctgggaaa aattcttgat cctttggctg ataaattact
     4141 ttttataatt gcttttgtgt ctctgtcact gaactatgcc ttaccagttt gggtggcgtg
     4201 gattgttgtt ggttgttata taattttatt gtcaggggga gtggtttttg tcaaaaaggg
     4261 aatttctatt ttaaagcata gctggctggg gagaggcgct ttcgctttgc aggcagttgt
     4321 cgtggccggt gtccttttaa gttattccgg acaagcctgg ctgacaatta taatctattt
     4381 gatggttttc ctgacagcca gcacctgcta ttattatctg gcccagggtt ttaaattatt
     4441 aaaaaaataa catgatcaac tggacaatca agcaggaaat cggc
//
LOCUS       DASWHZ010000037         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_89078, whole genome shotgun sequence.
ACCESSION   DASWHZ010000037 DASWHZ010000000
VERSION     DASWHZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89078"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VGA49_00895"
     CDS             <1..978
                     /locus_tag="VGA49_00895"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9664357.1"
                     /translation="NFSPPPANSSDKPVLRGENTSETRVDVCTLTGKLATIYTPPTYT
                     EHRIYKEVHNILHYVSKNNPQGPAPADPANADIEYANWEEAVQIWIENNKEEFLKLIQ
                     PDPDQPIDPDVVIILNESPPAEEDDDHRPELQPQIRITSPENNQLFNDNLISVSVDAE
                     APGGVGRLEYYIDDFLYYSDLTFPFGETSFDITDVPNGFHRLKVIAYDPIDNFKEDQI
                     QFQLQAPKPALTISWAEPANSLSLTAENFPLTLTVALSQMDNVQKVNFYAKENRGQLI
                     NISADENLENNQASIVWEEPPGPGTYKLQAEALTLTETVPGAGLIVIIQ"
     gene            975..1103
                     /locus_tag="VGA49_00900"
     CDS             975..1103
                     /locus_tag="VGA49_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664358.1"
                     /translation="MNNRAVPPSAGFIICTPLHPRGKLRGIRGQIIKNPSPFGGGF"
     gene            complement(1526..2629)
                     /gene="dnaN"
                     /locus_tag="VGA49_00905"
     CDS             complement(1526..2629)
                     /gene="dnaN"
                     /locus_tag="VGA49_00905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9664359.1"
                     /translation="MKFTSTQENLNQGLMIVSHIAAKTTSLPILNNVLIKAGGGVIQL
                     ETTNLELGVTCQVRGKVEAEGSFTVGAKLFADYVNLLPRENVNIELNDQTLKVSCKNY
                     QTKINGAPAEDFPLIPQLEKKEPAKLKIADFKKALSQVVFAAALDETRPEINGVFLNF
                     KGNKLTLAATDSYRLAEKVLNLDQGLAGDKNLIVPLRTFQELQRILTDGLEGDTIEIY
                     LGDNQILFLFNGVELISRLVEGKYPDYKQIIPTDFKTTAVASVSELAKVIKTASLFCR
                     TGINDINLKVIPNKVTVSSANSQLGENRTEIETETSGDDNEIVFNFRYLLDGLNNIDT
                     NEVELKIIDPNSPGLLKSREGQDYVYIVMPIKQ"
     gene            complement(2881..>3881)
                     /gene="dnaA"
                     /locus_tag="VGA49_00910"
     CDS             complement(2881..>3881)
                     /gene="dnaA"
                     /locus_tag="VGA49_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9664360.1"
                     /translation="FENYIVGKSNELAHAACRAVAERPGIVYNPLFLYGGVGLGKTHL
                     VQAVGHEILKNDPATKIIYVSCEKFTNDYIDAVKNGRGKEFQDRYRNVDILLIDDIQF
                     ITGKERTQEAFFHTFNALHQNNKQIVLTSDRPPKAIATLEKRLLSRFEWGMIADIQNP
                     DLETRIAILEAKCQEKDYSLDSEIINYLASNIQTNIRELEGALNRIIAQHQLQNIEPS
                     LPNVKKLISKIASTFQNRSLTPKQILEIVADFYDIDIALVVGNSRKKELVVPRQITMF
                     LMREELRSSYPMIGNELGGRDHTTAMHACDKITKAFENDEKLRQEILSIKQRIYNS"
BASE COUNT         1071 a          854 c          803 g         1153 t
ORIGIN      
        1 aatttcagcc cgccgccggc caatagttca gacaagcctg tcctgcgggg agaaaatacg
       61 tcagaaaccc gggtggacgt ctgtacttta accggcaagc tggccactat ttacacgccg
      121 ccgacttaca ctgaacatcg aatttacaaa gaagttcata atatcctgca ttatgtcagc
      181 aaaaataatc cccagggtcc ggctccggct gacccggcga acgctgacat tgaatacgcc
      241 aactgggaag aagctgtcca aatctggatt gaaaataata aagaagaatt tttaaaacta
      301 attcagcctg atcctgacca gccaattgat cctgatgtcg tgatcatttt gaatgagtcg
      361 ccgccagccg aagaagatga tgaccaccgg cccgagcttc agccccaaat cagaatcacc
      421 tcgcctgaaa acaatcagct cttcaatgac aatttaattt ccgtttccgt tgacgccgag
      481 gccccgggcg gcgtcggccg gctggaatat tatattgacg actttttata ttattctgat
      541 ctgacctttc cttttggtga aacctctttt gatataactg acgtgccaaa cggatttcac
      601 cgcctgaagg taattgctta tgatccaatt gataatttta aagaagatca aattcagttc
      661 cagctacagg ccccaaaacc ggccctgact atcagctggg cggaacctgc caatagctta
      721 agcctgacag ctgaaaactt tccattgact ctgacggttg ccttgtccca gatggacaat
      781 gttcaaaaag taaactttta tgccaaagaa aatcggggac aattaataaa tattagcgct
      841 gacgaaaacc tggaaaacaa ccaggcctca attgtctggg aagagcctcc cggaccggga
      901 acctataaac tgcaggctga agccctgact ttaactgaaa ccgttccggg ggctggtctt
      961 atcgtcatca tccaatgaat aaccgcgccg tcccgccgtc ggcggggttt attatctgca
     1021 cgccgcttca tccccgcggc aagctgcggg gtattcgcgg gcagataata aaaaatccct
     1081 cgccgtttgg cggggggttt taattttatc ttaaaatttt caggaaaaag ttggaggata
     1141 gaaaacagct ttgcttgctg ttataaaaac agctgccaaa gccaggggta tcttcaaaag
     1201 caaattccag tcagaccgaa ataaacttta tccaatacta gaggctgatt tttttgtatt
     1261 taatgtttta tatcagccca aaactctact cctccaattt tctcctgata aatattttat
     1321 tgtcaacaaa ccaccatatc acaataatca atttttgtca atatcagcga ctggcttttg
     1381 ataaaatgtg cacaagctgt taattatcaa attattctaa tcttagctta aaaaagatgc
     1441 ttttctgacc ttttcccagt attgtccaca aaaatttcac ctaacaaaaa acaggagcta
     1501 agccctgttt tatttttagt caaatttact gcttaatcgg cattactata taaacatagt
     1561 cctgcccttc ccgggatttt agcaggcccg ggctgttggg atcaataatt ttcagctcaa
     1621 cttcattagt gtctatatta tttagcccgt ccagcaaata acgaaagtta aaaacaattt
     1681 cattgtcatc gccggaagtt tctgtttcaa tctctgttct gttttctccc agctgactgt
     1741 tggccgaact gacagtaact ttgttgggaa tcacttttaa atttatgtca ttaatcccgg
     1801 tccggcaaaa caagctggcg gttttaatga ctttggccag ttcagagacg ctggccacgg
     1861 ctgtggtttt aaaatctgtc ggaataattt gtttgtagtc ggggtatttg ccttcaacca
     1921 gcctggaaat caattccacg ccgttaaata aaaacaaaat ttgattatcg cccaaataaa
     1981 tttcaattgt gtctccctcc aggccgtccg tcaggattcg ctgcagctcc tgaaatgttc
     2041 tcagcgggac tattaaattt ttatcaccgg ccaggccctg gtctaaattc aaaacttttt
     2101 ccgccaaacg atagctgtct gtcgcggcca aagtcagctt attgcctttg aaatttaaaa
     2161 agacaccgtt gatttccggc ctggtttcat ccagggcggc cgcgaagact acctggctta
     2221 aggccttttt aaaatcagct attttaagtt ttgccggttc ctttttttcc agctggggaa
     2281 tcaaaggaaa gtcttccgcc ggcgcgccat ttatttttgt ctgataattt ttacaggaaa
     2341 cctttagggt ttggtcgttt aattcaatat tgacgttttc ccgcggcaga aggttaacat
     2401 aatcagcaaa tagtttggcg ccaaccgtaa aggagccctc ggcctcaact ttccccctga
     2461 cctggcaggt aacgcccaac tcaagattag ttgtctccag ctggataaca cctcccccgg
     2521 cttttattaa aacattattt aaaattggca gtgaggttgt tttggcggca atatgactaa
     2581 ctatcattaa tccctgattc agattctcct gcgttgacgt aaatttcata ggtattattt
     2641 attatataaa ttaatttaaa aaatcaataa tagataataa ggagtgttaa taagttgata
     2701 accacttagg agccttttgt ttaaagggtt ttttaaaggg tgttttgttt aaaaacatgt
     2761 gaactatttt taataaaatg tttataactg tttaggctgg ctagccgttg tttataacca
     2821 gtcagtttat caacttttta ttaaaaataa attaaatgtt tttgaacaac ttgtaaacag
     2881 ctagctgttg taaattcgtt gcttaattga caggatttcc tggcgaagtt tttcgtcatt
     2941 ttcaaaagct ttggtaattt tatcacaggc gtgcatggcg gtggtgtggt cgcggccgcc
     3001 cagctcgttg ccaatcattg gataagaact gcggagttct tctcgcatta gaaacatcgt
     3061 aatttgtcgg gggacgacca gttccttttt ccggctgttg ccaacaacca gcgcaatgtc
     3121 aatatcataa aaatcagcca caatttcaag aatttgtttg ggagtcagcg accggttctg
     3181 gaaggtggag gcaattttgg aaatcagctt tttgacattg ggcagagaag gctcaatatt
     3241 ttgcagttga tgctgggcaa taattcggtt taaagcgcct tccagctccc ggatgttggt
     3301 ttgaatattg gacgccagat aattgataat ttcagaatca aggctgtagt ctttttcctg
     3361 gcacttggct tctaaaatgg ctattctggt ttccagatca gggttttgaa tgtcagcgat
     3421 catcccccac tcaaaccggg ataataacct tttttctagg gtggcgatcg ctttgggcgg
     3481 ccggtcggaa gtcagcacga tttgcttgtt attttgatgg agggcgttaa aggtgtgaaa
     3541 aaaagcttcc tgggttcgct ctttgccggt gataaactga atgtcgtcaa tcagcaggat
     3601 gtcaacattg cggtaacggt cctgaaattc tttgccgcgg ccgtttttaa cggcgtcaat
     3661 ataatcatta gtaaattttt cgcaggagac atagataatt ttagtggcag gatcattttt
     3721 taaaatttca tggccaacag cctgaactaa gtgggttttg cccagaccaa cgcccccgta
     3781 aaggaaaaga ggattataaa caatccccgg cctttcagcc accgcccggc aggcggcgtg
     3841 cgccagctca ttgcttttac cgacaatata attttcaaaa a
//
LOCUS       DASWHZ010000038         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3407640, whole genome shotgun sequence.
ACCESSION   DASWHZ010000038 DASWHZ010000000
VERSION     DASWHZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3407640"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /gene="dnaE"
                     /locus_tag="VGA49_00915"
     CDS             complement(<1..1902)
                     /gene="dnaE"
                     /locus_tag="VGA49_00915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9664361.1"
                     /translation="MPFVHLHTHSHYSLLDGLAKIDQLVAKAVEHNMPALALTDHGAM
                     YGSIEFYQACLKAGIKPIIGLEAYVAPNGMTNKRPRIDDKRHHLVLLAQNEAGYKNLI
                     KLTTKAHLEGYYYKPRIDWETLTGHKDGLIVLSACLAGEIPGLILNGQPDKARATALK
                     YQETFGPDNFYLEVQRHPAILQQEPVNQALFKIGQELGIDVVATNDIHYASQEDAVAQ
                     DTLLCIQTNRKIQETDRMKMSGDDFYFKSPAYIKDQFKDHPEVIDNTLKIAGLCNLEL
                     KFGQVTLPYFEVPSGETGHSYLKKLAVEGLKKRYNINYHQPLSGDKEILDRFEYEMSV
                     IEKTGFAPYFLIVQDFVNWAKDQGITVGPGRGSAAGSLVSYATNITNIDPLKYDLLFE
                     RFLNPERISMPDIDLDFADTRRDEVIRYVESKYGQDKVAQIITFGTMAARGAIRDAGR
                     ALDLPYAYCDQVAKMIPMGHKLNQALEISTDLKALYQGDQDCRTLIDRARKLEGVARH
                     TSTHACGVLITKDSLDNYVPLQYSSTHDQALVSQYSLHPIEDLGLLKMDFLGLKNLTI
                     LENAVEIVEKVRGLKVNLDKIPVDDQKTFQLLQRGETTGVFQLESSGMRRYLIQLKPN
                     DFEDIIAMIS"
BASE COUNT          544 a          483 c          468 g          563 t
ORIGIN      
        1 agaaatcatg gcgatgatat cctcaaagtc attcggtttg agttgaatta aataccggcg
       61 catgcccgaa ctctccagct gaaaaacacc ggtggtttct ccccgctgta aaagctgaaa
      121 agttttttga tcatccaccg gaattttatc aagattaact ttcagtcccc tgaccttttc
      181 aacgatctcc acggcgtttt ccaaaattgt cagattttta agccccaaaa aatccatttt
      241 cagcaatcct aaatcttcaa tgggatgaag agaatattga gaaaccaaag cctggtcgtg
      301 ggtggagctg tactgaagcg ggacgtaatt atcaagcgaa tctttggtaa ttaaaacgcc
      361 gcaggcgtga gtggaggtat gccgggcgac gccttccagc tttcgggccc gatcaattaa
      421 agtccggcag tcttgatcgc cctgatagag cgctttaagg tcagttgaaa tttccagagc
      481 ctggttaagc ttatggccca tcggaatcat tttggcgacc tggtcgcagt aagcgtaagg
      541 caaatccaaa gcccggccgg cgtctctgat cgctccccgg gcggccatcg tcccaaaagt
      601 aataatctga gcgactttgt cctggccgta cttgctttca acatacctga tgacttcgtc
      661 ccgccgggta tcggcaaaat ccagatcaat atcaggcatg gagattcttt ccggatttaa
      721 aaatctttca aacagcagat catatttcag cggatcaata ttagtgatat tagtggcgta
      781 agaaacaaga ctgccggcgg ccgatccccg gcccggacca acggtgattc cctgatcctt
      841 ggcccagttg acaaaatcct gaacaatcaa aaaataagga gcaaacccgg ttttttcaat
      901 gactgacatt tcatattcaa atcgatccaa aatttcctta tctccggaca gcggctgatg
      961 ataattaata ttatatcttt tctttaatcc ctcaaccgcc agttttttta aataactatg
     1021 accggtctct cctgacggca cttcaaaata aggcaaagtc acctggccaa attttagttc
     1081 cagattacac aacccggcaa tctttaaagt attgtcaatc acttccggat gatctttgaa
     1141 ctggtcttta atgtaagccg gcgatttaaa ataaaaatca tcgcctgaca ttttcatccg
     1201 gtcagtctcc tgaatcttcc ggttggtctg aatgcagagc aaagtgtcct gagcgacggc
     1261 gtcttcctgg ctggcgtaat ggatatcatt agtggccact acgtctatcc ccagttcctg
     1321 gccaatttta aacaaagcct ggttaaccgg ttcctgctga agaatcgccg gatggcgctg
     1381 aacttccaga taaaaattat ccggcccaaa tgtctcctga tattttaaag cggttgcccg
     1441 ggctttatcc ggctggccgt ttaaaatcaa accgggaatt tctccggcca ggcaggccga
     1501 caaaacaatc aggccgtctt tatgcccggt taaagtttcc cagtcaatcc ggggtttata
     1561 ataatagccc tccaaatggg ctttggtggt cagtttgatt aagtttttat agccagcttc
     1621 gttttgagcc agcaaaacca gatgatgccg cttgtcatca atccgcggcc gtttattggt
     1681 cattccattg ggcgcgacat aagcttccag gccaatgatc ggcttgatcc cggccttaag
     1741 gcaggcctgg taaaactcaa ttgaaccgta catggcgccg tgatcagtca aagccaaagc
     1801 tggcatgtta tgttcaaccg ccttggcgac cagctggtca attttggcca agccgtccag
     1861 taaactgtaa tgagaatggg tatggaggtg gacaaatggc atggggtaaa agggataaga
     1921 gggataaggg ggtaaaggtt ctagttttaa gttattagtt ttagctgcca gtatggacag
     1981 aggtttggtt ttaggtgtgg cagccggtat ggtctgtggt tttagtttta ggttttttta
     2041 cctaaaccca caccctaa
//
LOCUS       DASWHZ010000039         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1587070, whole genome shotgun sequence.
ACCESSION   DASWHZ010000039 DASWHZ010000000
VERSION     DASWHZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587070"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1067
                     /locus_tag="VGA49_00920"
     CDS             93..1067
                     /locus_tag="VGA49_00920"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX9664362.1"
                     /translation="MASYQHFTQEERIMLSTLKRRGLTQQEMARELGKDQSAISRELS
                     RNKQEDGSYHAGYAKQAANRRRKEANQVLKRIANDPWLEKYIIKRLKIYWSPEQIAGR
                     ARRDYEVMVCHETIYQYIYNDRPDLKKYLRCQKGKYRRRYGTKIREKQREEAKKTRID
                     RRPKVIERRERLGDWEGDTIIGQRGTGSLATHVDRKSGYTFIDYVARANAEAIRETVV
                     KRFTKLPKQKKHTITYDNGTEFSEHEMIGRETKIDIYFAYPYHSWERGTNENTNGLIR
                     QFFPKKSSFADITNTDTRRAERLLNTRPRKRLMYLTPVEVFRKNMHLT"
     gene            complement(1042..2565)
                     /locus_tag="VGA49_00925"
     CDS             complement(1042..2565)
                     /locus_tag="VGA49_00925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9664363.1"
                     /translation="MGKKNILYLSAIIIFAAGLRILALTTQGSFWFDEIFTVYFAGLD
                     LPEMFSFIIYDHNPPVHFLLIHYWLKLFGSNEIIVRLSSLIFGLANIGLIYLVGQQLF
                     SKRIGQLAGFLGAISLFQIYYSVEARMYSLLLFLTLLSFYFFWRLLNQYKFKNLILYL
                     GFTVLALLTHLTGLFVLLIQNAFVLYLYFQKELKLNFKKWFFGQLAVVIVFGIWFGPM
                     VYLKLQEGLNILQGWYFTQTTRHFFLENLNNFFIFGRYQEFINLILQIIILGLLILAV
                     IRISRPDKRFIVDFDRSGQIILLLLWLFLPLAVGFIFNVNVTKYYIIASPALYLLAAK
                     GLDNLKLTKKYLALFLIIVFLLIWPLAPHNVTAAWPEVIQYIEDQSTDNSKVIANPFI
                     LTLPLKFYDLQGVTLEGFYPVDDEPNLDLRIVKKNWFPQITEDNAEKLQQATYNFDRI
                     LLVTFYVDQSRQDDFVKKWFLKNGWQITDERLFPADVGVITLIRSQTVNSRSSAYFF"
     gene            complement(2742..>3312)
                     /locus_tag="VGA49_00930"
     CDS             complement(2742..>3312)
                     /locus_tag="VGA49_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX9664364.1"
                     /translation="TADEVLRLKGQYPVELIKREKRLGIGSAYLNGFKYALEQGADLI
                     FEMDADLSHQSQAIPQFLKAAENADVVIGSRKIPGGQIIGWGLWRQFCSAGAMWFSRL
                     ILGLKTRDVTSGFRCYKREALNSFDLSKIKSNGYAFQEELIWLCEKNNCKIKEIPITF
                     IDRQSGKSKLTGRDVAEFFSSMVRLKLDK"
BASE COUNT         1185 a          688 c          593 g          846 t
ORIGIN      
        1 cggtgtattc taaccgcaag cgcataaaac accaaaagac gcccagggtt aggtataata
       61 gaaatattgt actaactatt aacctaacac ctatggcgtc ttatcaacat tttacccaag
      121 aagagagaat tatgctatcc actctaaaac gcagagggct aacccagcaa gagatggcca
      181 gagagcttgg caaagaccag tcagccatct ctcgggaatt aagcagaaac aaacaagaag
      241 acggcagcta tcacgcgggg tatgcgaagc aagcagccaa tcgacgaaga aaagaagcta
      301 accaagtctt aaaaagaata gccaacgatc cgtggcttga aaaatatatt atcaaaagat
      361 taaaaatata ttggtcgccg gaacagatag ccggacgagc gcgaagggat tacgaagtca
      421 tggtctgcca tgaaactatc taccaatata tctacaacga caggcctgat ttgaaaaaat
      481 atttacgctg tcaaaagggt aaataccgga ggcgatatgg tacgaaaata cgagaaaaac
      541 aaagggaaga agcaaagaag accaggattg acagacgacc gaaagttatt gaaagaagag
      601 agcgtttggg cgactgggaa ggcgatacga tcatcggcca gagaggaact ggttcgctgg
      661 caacgcatgt tgaccgcaag agcggctaca ccttcataga ctatgttgcg cgagcaaacg
      721 cagaggcaat cagagagacg gtcgtcaaac gatttacaaa actgccaaaa caaaaaaagc
      781 atacgattac gtatgacaat gggacagagt tttcagaaca cgaaatgatt ggacgggaaa
      841 caaaaattga catatatttc gcctatccat atcactcatg ggagcgtgga acgaatgaaa
      901 atacgaatgg actcatcagg cagtttttcc caaagaagtc atcatttgcc gatattacaa
      961 acacagacac tagacgagct gagcgtctgc ttaacaccag accgcgcaaa agattaatgt
     1021 acctaacccc agtggaggtc tttagaaaaa atatgcactt gacctagaat ttacggtctg
     1081 actccggata agggtgatca ccccgacatc ggccggaaaa agacgctcgt cagttatttg
     1141 ccagccgttt ttcaaaaacc attttttgac aaaatcatcc tgccggctct gatcaacata
     1201 aaaagttact aataaaatcc ggtcaaaatt ataagtcgcc tgctgaagtt tttcagcgtt
     1261 gtcttctgtt atctggggaa accaattttt tttgacgatt cttaaatcca gattaggttc
     1321 atcatcaacc ggataaaacc cttccagcgt cacgccttgt aaatcataaa atttcaacgg
     1381 caaagttaaa ataaacggat tggcaatcac tttactgttg tcagttgact gatcttcaat
     1441 atattgaatc acctccggcc aggcggctgt cacattgtgg ggtgccagcg gccagattaa
     1501 cagaaagaca attatcaaaa acaaagctaa atatttttta gttaatttta aattatccaa
     1561 acccttcgcc gccagtaaat acaaagccgg cgaagcgata atataatatt tagtaacatt
     1621 aacattaaaa ataaaaccaa cggccagcgg caggaacagc cataagagca ataaaattat
     1681 ttggcccgac cggtcgaaat ccacgataaa ccgtttatcc ggccggctga tcctgataac
     1741 cgctaaaatc aataatccta aaattattat ttgcagaatt aaattaataa attcctgata
     1801 cctgccaaaa ataaaaaaat tatttaaatt ttccagaaag aaatggcggg tcgtctgggt
     1861 aaaataccag ccctgtaaaa tattcaggcc ctcttgtaat ttcaaataga ccattggtcc
     1921 aaaccagatt ccaaaaacaa taactactgc cagttgccca aaaaaccact ttttaaaatt
     1981 cagctttaat tccttttgaa aatacaaata aagaacaaaa gcgttttgaa ttaataaaac
     2041 aaacaaaccg gtcagatgag ttaataaagc taaaaccgta aaacccaaat ataaaattaa
     2101 atttttaaat ttatattgat taagaagccg ccagaaaaaa taaaaagaca gcaaagtcaa
     2161 aaataacaac aagctgtaca tccgggcttc aactgaataa taaatctgaa ataaggaaat
     2221 cgctcccagg aaaccggcca gctggccaat tctttttgaa aaaagctgtt gaccgactaa
     2281 ataaattaaa ccaatattag ccaggccaaa aatcagcgat gataatctga caataatttc
     2341 atttgaacca aacagcttca accagtaatg aatcagcaaa aaatgaaccg gcggattatg
     2401 atcgtaaata ataaatgaaa acatttctgg caggtcaagg ccggcaaaat aaaccgtaaa
     2461 aatttcatca aaccaaaaac taccctgggt ggttagcgcc aaaatcctca acccagcggc
     2521 aaaaatgata atggcgctta gatataaaat gttttttttg cccatgaaat tttaaattca
     2581 aaattcgaaa tcctaaataa attttaatga tcaaaattta aaattctaaa cttggtgaaa
     2641 aaagttttgg atttaaaatt ttgtatttcg tacttattta ggatttctga ttttggattt
     2701 ggtgcttttt aaaaattaat ccttttaacg ttgctttcat tttacttatc caattttaat
     2761 ctaaccatgc ttgaaaagaa ctccgccacg tccctcccag tcaactttga ttttcctgat
     2821 tgacggtcaa taaatgttat cggaatttct ttaattttac agttgttttt ttcacaaagc
     2881 cagatcagtt cttcctgaaa agcgtagcca ttgcttttga tcttgcttaa atcaaatgaa
     2941 tttaaagctt cccttttata acaacgaaaa ccgctggtca cgtctctggt ctttaagccc
     3001 aaaattaatc ttgaaaacca catcgccccg gcgctgcaaa attgccgcca taacccccag
     3061 ccgataattt ggccgcctgg aatttttctg gagccaatca ccacgtcagc attttcagct
     3121 gcttttaaaa attgagggat ggcttgagac tgatgagata aatcagcgtc catttcaaaa
     3181 atcaaatctg ctccctgctc cagagcgtat ttaaaaccgt tcaaataagc gctgccaatc
     3241 cccagtcttt tttcacgctt aatcagctca accggatatt gtccttttaa ccttaaaact
     3301 tcatcagccg tg
//
LOCUS       DASWHZ010000040         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3070058, whole genome shotgun sequence.
ACCESSION   DASWHZ010000040 DASWHZ010000000
VERSION     DASWHZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070058"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VGA49_00935"
     CDS             complement(<1..409)
                     /locus_tag="VGA49_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9664365.1"
                     /translation="MKGIILAGGTGSRLYPLTRVTNKHLLPVYNQPMIYYPINTLVKA
                     GIKDVMIVSGKGHAGHFLELLRSGEEFEANFSYGVQEQPGGIAQALGLCQSFADDQKV
                     AVILGDNIIQDNIRPAVDHFKGSDRGAKVFLKEV"
     gene            complement(422..1429)
                     /gene="rfbB"
                     /locus_tag="VGA49_00940"
     CDS             complement(422..1429)
                     /gene="rfbB"
                     /locus_tag="VGA49_00940"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010494539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX9664366.1"
                     /translation="MKLLITGGAGFMGSNFIKYIFKKYPGYKIINLDKLTYAGNLDNL
                     TDIQVNPDYKFIKGDIADEKLVNTIIEQEKPDAVINYAAETHVDRSIMEPRAFLYTDI
                     IGTFNLLEAVRKYGLKRMVQISTDEVYGSIEAGEFTESSPFMPNSPYSASKAGGDHLC
                     RAYWKTYQTPVIVTHSCNFYGPHQYPEKLIPLFITNLLEDKKVPVYGDGLQTREWIFT
                     NDHCQAIDQVLHQGVIGEAYNIGTRQRQKNIEITRLLLDMLGKSENYIEFVTDRPGHD
                     QRYALNSDKLTKDLAWNAAVAFETGLEQAVDWYKTNRWWWEKIKNGQYLEYYKQQYEQ
                     R"
     gene            complement(1456..1881)
                     /locus_tag="VGA49_00945"
     CDS             complement(1456..1881)
                     /locus_tag="VGA49_00945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9664367.1"
                     /translation="MSDCIFCKIIKGELPSSNVYEDESVIAFLDHSPVNPGHALVVSK
                     EHHPDLVSTPPQVISKLIEAAQTIAPAVLAATDSQGFNLNLNNGSAAGQVVNHIHFHI
                     VPRHPNDGLKLWPGQPYQAGEIEELADKIRNNISIIRIN"
     gene            complement(1965..>2383)
                     /gene="eno"
                     /locus_tag="VGA49_00950"
     CDS             complement(1965..>2383)
                     /gene="eno"
                     /locus_tag="VGA49_00950"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088226.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX9664368.1"
                     /translation="YPFLLIEDPLDQDDWDNWQVCTKKLGKKILIIGDDLFVTNVERL
                     KQGIKLGVANSILIKPNQIGSLTETIETIKLAQKNKYKVIISHRSGETCDDTIADLAV
                     AVNADYAKIGSLSRSERVCKYNRLMEIERDIKTGEH"
BASE COUNT          661 a          532 c          461 g          729 t
ORIGIN      
        1 ttacttcttt taaaaatact ttggctcccc tgtcagatcc tttaaaatga tcaaccgccg
       61 gccggatatt atcctgaata atattgtccc ccaaaattac ggccactttt tgatcatcag
      121 caaagctttg gcagagcccc aaagcctggg cgatgccgcc gggctgttcc tgaacgccgt
      181 aggaaaaatt agcttcaaat tcttcgcctg atcttagcaa ttccaaaaaa tgaccggcgt
      241 ggcctttacc ggagacaatc ataacatctt taatgccggc cttaaccaaa gtatttattg
      301 gataataaat catcggctgg ttataaaccg gcaataaatg cttattggtc acccgggtta
      361 agggataaag ccgggaaccg gtcccgccgg ccagaataat gcctttcata ttaataacgc
      421 gttatctttg ctcatattgc tgtttgtaat attccagata ctggccgttt ttaattttct
      481 cccaccacca gcggttagtt ttataccagt caaccgcctg ctccaaaccg gtttcaaagg
      541 cgactgccgc gttccaggcc aaatctttgg tcagcttgtc gctattgagg gcgtaacgct
      601 ggtcatggcc cggccggtcg gtgacaaatt caatgtaatt ttcacttttg cccagcatat
      661 ccagtaataa tctggtaatt tcaatatttt tctggcgctg tctggtgcca atattatagg
      721 cctcgccaat tactccctga tgtaaaacct gatcaatcgc ctggcaatga tcattggtaa
      781 aaatccattc ccgggtctga agaccgtcgc cgtaaaccgg gaccttttta tcctccagca
      841 aattggtgat aaacaacgga attaattttt caggatattg atgcgggccg taaaaattac
      901 agctatgggt gacaatcacc ggcgtttgat aagttttcca gtaggcccga caaagatgat
      961 caccgccggc tttggaagct gaatagggac tgttgggcat aaacggcgag ctttccgtaa
     1021 attccccagc ctcaatgctg ccgtaaactt cgtctgttga gatttgaacc attcttttta
     1081 aaccatattt tcggacagct tccagtaaat tgaaagtgcc tataatatca gtgtaaagaa
     1141 aagcccgggg ttccataatt gaccggtcaa catgcgtttc agcggcataa ttaattacgg
     1201 catccggttt ttcctgctcg atgatagtat taactaattt ttcatcagca atatcgcctt
     1261 tgataaattt ataatccgga ttaacctgaa tatcagttaa attatccaga tttccggcgt
     1321 aagttaattt atccaggtta ataattttgt aacctggata ttttttgaaa atgtatttaa
     1381 taaaattgga gcccatgaac ccggctccgc cagttatcaa tagtttcata aataccaatt
     1441 atcgattcta atatactaat tgattctaat tatactaata ttatttctta ttttatcagc
     1501 taactcttct atctcccctg cctgatatgg ctggccaggc cataatttta aaccgtcatt
     1561 tggatggcgg ggcacaatgt gaaagtggat atgattaaca acctggccgg cggctgaacc
     1621 gttgtttaaa ttcaaattaa atccctggct gtcagtggcc gctaaaaccg ccggagcaat
     1681 cgtttgagca gcctcaatta atttggagat tacttgtggg ggggttgaga ctaaatcagg
     1741 gtgatgttct ttggagacaa ctaaagcatg cccgggattg accggcgaat gatccaaaaa
     1801 ggcaatcacc gattcatctt cataaacatt tgaagacggc agttcgcctt tgataatttt
     1861 gcaaaagatg cagtcagaca tattcattaa agcgcaaaac attaaagcat gaaagcatta
     1921 aaacatgaaa acaaattgtt ttaatattcc cttgttttaa tgttttaatg ttctcctgtt
     1981 tttatgtctc tctcaatttc catcaaccta ttatatttac aaacccgttc gcttctggac
     2041 aggctgccga ttttggcgta atcagcgtta acggccaccg ccagatcagc gatcgtgtca
     2101 tcgcaggttt cgcctgaccg gtgggagata atcactttat atttgttctt ttgagccagt
     2161 ttaatagttt caatcgtttc cgtcaaactg ccgatctgat tgggtttgat taaaattgaa
     2221 ttggccactc ccagtttaat cccctgtttc agccgttcaa cattggtcac gaacaaatca
     2281 tcgccgataa ttaaaatctt tttccccaat tttttagtgc aaacctgcca gttatcccaa
     2341 tcatcctgat ccaaaggatc ctcaattaat aaaaaggggt att
//
LOCUS       DASWHZ010000041         4479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1204985, whole genome shotgun sequence.
ACCESSION   DASWHZ010000041 DASWHZ010000000
VERSION     DASWHZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204985"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2625)
                     /locus_tag="VGA49_00955"
     CDS             complement(<1..2625)
                     /locus_tag="VGA49_00955"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9664369.1"
                     /translation="MKKEIPKIYDAAKVEDDIYRQWEESGLFNPEVCIRDGFTSAKAE
                     PFTIVLPPPNVTGTLHLGHASMLAFEDIMVRYHRMKGDRTLWIPGTDHAAIATQSKVE
                     KILWDKNKQTRHDLGRKNFLKLVEGFASESHDIIVHQTKKMGASLDWSREAFTLDEPR
                     TLAVRTAFKKMYDDGIIYRGHRLVNWDPRLQTTVSDDEVEYIEKKSPFYYLKYGPFTI
                     ATARPETKFGDKYVVVHPRDKRYKKYKHGQKIDLEWINGPVTATVIKDEAIEMELGTG
                     AMTITPWHDLTDFEIAGRHNLDKEQIIDFDGQLLPLAGEFAGMDIKEARQKIVSKLEK
                     KGLIEKVDQDYLHNVAANSRGGGLIEPQIMEQWFIDVNKEFKRSSKKVTLKILMKEAV
                     NSGKIKIIPERFTKVYYHWIDNLRDWCISRQIWYGHQVPVWYKKKFKVQSSKFQPKAD
                     PPPADKVQSSKFKATDENAEEIYVGLKEPEGEGWRQDSDTLDTWFSSGLWTFSTLGWP
                     EETEDLKTYHPTTVLETGYDILFFWVARMILMTEYLLGEAPFKYVYLHGLVRDEQGRK
                     MSKSLDNIIDPLDMILKYGTDPVRLSLVVGTAPGNDLRLYDEKIAGYRNFVNKLWNIS
                     RYILLTVKSVETDTKKPRPASLADKWILSRLNRLKKEVTDNIEQFNFSYAAEQLYDFT
                     WQELADWYVEVSKIEGQKDQILIHILKNLLRLWHPYTPYVTEEIWKNLNPKNLLMVSR
                     WPAVDKKLIDKKAEKDFELIRQIITAVRNARAENKIAPAKKIKAVVYGKDQAGLIEDQ
                     SEIIKTLARLSEIEVKTGSARQPPGKSIGVFVGKTEIYLPVSDLIDLAQEKARLEKEL
                     GKMRQLTSQLDKKLKNKKF"
     gene            3214..4392
                     /locus_tag="VGA49_00960"
     CDS             3214..4392
                     /locus_tag="VGA49_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX9664370.1"
                     /translation="MSLTLTEQVQEAIRNSNNILITFKKINNGDSLSAALGLALILKK
                     LRKKADIIADQFVISQKYNFLPAIDLIQTESPPLNKFLISLDISKNKIDEFNYNITDT
                     RLNIFITPKNGNFTAGDVSSSASEFKYDLIFTVDTPDLDMLGSIYDNNTQFFYNTPII
                     NIDHEADNEHYGQINLIDLTATSASEIIFNLFEKIANETMDEDIATCLLTGLICKTRS
                     FKSAQITPKSLNIVSKLLAIGARREEIIHNLYQTKSIDTLKLWGKVLSSINHDQNLKL
                     VWAALKNHQPQNINEENLSEIIDELMSTCPDAQTIILMYPNGLNSTNSLVYTEKNINA
                     LNLTKKFKPSGSKNFVKFIMENTPVDQAEKEVVEEVKNSLANNPALGEQTGQGNVITI
                     "
BASE COUNT         1247 a         1060 c          848 g         1324 t
ORIGIN      
        1 aaatttttta ttttttagct ttttgtccag ctgactggtt aattgtcgca ttttccccag
       61 ttctttttca agtcgggctt tttcttgagc cagatcaatc aaatccgata ctggcagata
      121 aatttctgtt ttacccacaa aaactccgat tgatttgcca ggcggctgcc gggcagaacc
      181 ggtcttaact tcaatttcag ataaacgagc cagggtttta attatttcag attgatcctc
      241 aattaagccg gcctgatctt taccataaac aacggcttta atctttttag ccggagcgat
      301 tttattttca gcccgggcgt ttctgacagc cgtgatgatt tgccggatca gctcaaaatc
      361 tttttcagct tttttatcaa ttaacttttt atcaaccgcc ggccagcggc tgaccattaa
      421 taaatttttg gggttgagat tcttccaaat ttcttcggtg acataaggcg tatacggatg
      481 ccataaccgc agtaaatttt tcaaaatatg tattaagatt tgatcttttt gaccctcaat
      541 tttggaaacc tcaacatacc agtctgccag ctcctgccag gtaaaatcat aaagctgttc
      601 agcggcgtaa gaaaaattaa actgttcaat attgtcagta acttcttttt taagtctgtt
      661 taaccgggac aaaatccatt tatcagccag gctggccggt cgtggttttt tggtgtcagt
      721 ctccacggat ttaaccgtca gtaaaatata gcggctgata ttccagagct tattgacaaa
      781 attccggtag ccagcgattt tttcgtcata gagccggaga tcattgcccg gagcggtgcc
      841 aacaaccaaa cttaacctga ccggatcagt tccgtatttt aaaatcatat ccagcggatc
      901 aataatattg tccagcgact tggacatttt ccggccctgc tcatcccgga ccaggccgtg
      961 aagataaaca tatttgaaag gcgcttctcc caacagatat tcagtcatta aaatcattct
     1021 cgccacccaa aaaaataaaa tgtcatagcc ggtttccaac accgtcgttg gatgataagt
     1081 ttttaaatct tcagtctctt ccggccagcc caaagttgaa aaagtccaga gccctgaact
     1141 aaaccaggta tccagcgtgt cagaatcctg ccgccagcct tcaccctccg gttcttttaa
     1201 accaacgtaa atttcttcgg cattttcatc agtagccttg aactttgaac tttgaacttt
     1261 gtccgccgga ggcggatcag cctttggctg aaactttgaa ctttgtactt tgaacttctt
     1321 cttgtaccag accggcactt ggtggccata ccaaatctgc cggctaatac accagtcacg
     1381 taaattatca atccagtggt aataaacctt ggtgaatctt tcaggaataa ttttaatctt
     1441 tccggaatta accgcctctt tcatcaaaat ttttaaagta actttttttg aacttctttt
     1501 aaattcctta ttaacgtcaa taaaccactg ttccatgatc tggggctcaa tcaaaccgcc
     1561 tccccggcta ttagcggcca cgttatgaag atagtcctgg tcaacttttt ctatcaaacc
     1621 ttttttctcc agcttagaga cgattttctg ccgggcttct ttaatatcca tcccggcaaa
     1681 ctcgccggct aatggtaaaa gctggccgtc aaaatcaatg atttgttctt tgtccagatt
     1741 atgccgcccg gcgatctcaa aatcagtcag atcatgccag ggggtaatcg tcatcgcccc
     1801 ggtgcccagc tccatctcaa ttgcttcatc tttaataacc gtcgccgtaa ccggaccgtt
     1861 gatccattcc aggtctattt tttggccgtg tttatatttt ttatagcgct tgtcccgggg
     1921 atggacaacg acgtatttgt cgccaaactt ggtttccggc cgggcggtgg cgattgtaaa
     1981 cggtccgtat tttaaataat aaaacggcga ttttttttca atatattcaa cttcatcgtc
     2041 ggaaactgtt gtctgaagcc ggggatccca gttaaccagc cggtggccgc ggtaaataat
     2101 gccgtcatca tacatttttt taaaagcggt ccgaacagcc agcgttcgcg gctcgtccag
     2161 agtaaacgct tcccggctcc agtcaagcga agcccccatt tttttggtct gatggacaat
     2221 aatatcatga ctttccgaag caaagccttc aactaatttt aaaaaattct tccggccaag
     2281 atcatgccgg gtctgtttgt ttttgtccca aagaattttt tcaactttgc tttgggtggc
     2341 gatagcggcg tgatcagttc ccggaatcca caaagttcgg tcgcctttca tccggtgata
     2401 acgaaccatt atatcttcaa aagccagcat tgaggcgtgg cccagatgaa gcgtcccggt
     2461 aacattgggc ggcggtaaaa ctatcgtaaa cggttcggct ttcgctgaag taaatccatc
     2521 ccggatacaa acttcaggat taaacaagcc tgactcttcc cactgccgat aaatatcatc
     2581 ttcaactttc gcggcatcgt aaattttagg aatttctttt ttcatattta gctattttac
     2641 cataaacagc cgtctttctc aagcttgaca tttgtctgtt tttgtgataa gtttccctgt
     2701 tctggagaaa cttttttact tgcaatattc agccagctta tataaaataa attacaaagt
     2761 ccccttagag tcgcgggtgg ttttaaaacg caggcagatc ggagttatgg aaattccctg
     2821 tccgctcccc ggcttcagtc ccgcctctgg cggggctgtc gccggtggat ggcaggaaaa
     2881 atctctctcc tcatttccag cccttcggtt tacagcctaa gtttttatct caatccgcgg
     2941 ctttgagggg attttttatt tgccccgccg cggcggggca ggcacaaggc aagaggcaca
     3001 aggcaatagg gtaggaataa tttattagat gggaattagg cattagtaaa aacccttttg
     3061 ccttataccc atcaaataat tgtcccccgc ccaaggtggg ggacaccaac ccttgtgcct
     3121 attgcctagt accttgtgcc tttttccata tccacttgac atcatttcac ggccgggtta
     3181 atatattctg attacctaat taaccactca tctatgagtt tgacactgac cgaacaagtt
     3241 caggaggcta tccggaacag caataatatt ttaatcacct ttaaaaaaat caataacggt
     3301 gattctttgt cagccgctct gggtttggct ttgattttaa aaaagctccg taaaaaagcc
     3361 gacattatag ctgatcagtt tgtgatctcc caaaaatata acttcctgcc ggccattgat
     3421 ttaattcaga ctgaaagccc gcccctgaat aagtttctga tcagcctgga tatttccaaa
     3481 aataaaattg acgaatttaa ttacaatatt actgacaccc gccttaacat ttttattacc
     3541 cccaaaaacg gcaacttcac ggccggcgat gtcagctcat ccgcttcaga atttaaatac
     3601 gacttaatct ttactgttga cacgcctgac ctggacatgc tcgggtcaat ttatgacaat
     3661 aacacgcagt ttttttacaa cacgccaatc attaacattg accatgaagc tgacaacgaa
     3721 cattacggcc agatcaacct gattgacctg acggccactt ctgcttcaga aatcattttt
     3781 aacctgtttg aaaaaatcgc taatgaaact atggacgaag acattgccac ttgtctttta
     3841 accggactga tctgcaaaac cagaagcttt aaatcagccc agatcactcc aaagtcgctt
     3901 aatattgtca gcaaactgct ggcgatcggc gcccgccggg aagaaatcat ccataatctt
     3961 taccagacca aatcaattga caccctcaaa ctctggggca aagttttatc cagcatcaat
     4021 catgaccaaa atctgaaact agtctgggcc gccctgaaaa atcatcaacc gcaaaatatc
     4081 aatgaagaaa acctgtcgga aattattgat gagctgatgt caacttgtcc ggacgcccag
     4141 acaatcattt taatgtatcc caatggcctt aattccacga acagtctggt ttacacggaa
     4201 aaaaacatta acgccctcaa tttaaccaaa aaattcaaac cgagcggctc caaaaatttt
     4261 gtaaagttta taatggaaaa tacgccggtt gatcaggcgg aaaaggaagt cgttgaagag
     4321 gttaaaaata gtctggctaa taatccggca ctgggagagc agacggggca agggaatgtc
     4381 atcacaatat aatttgaaat acaaagtttt tattatctgt ctttaaaaaa tcctaaattc
     4441 taatatcgaa atcctaaata aattttaatg atcaaaatt
//
LOCUS       DASWHZ010000042         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2334541, whole genome shotgun sequence.
ACCESSION   DASWHZ010000042 DASWHZ010000000
VERSION     DASWHZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334541"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VGA49_00965"
     CDS             <1..1429
                     /locus_tag="VGA49_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX9664371.1"
                     /translation="NWWSRSGVPEKMPPGEPGGPDSEIFWDFGEKLGHHEASPFQAQP
                     CHVNCDCGRFLEIGNSVFMEYRKTKTGFEPLPQKNVDFGGGLERMAAAAQNDPDIFKI
                     DTFRPLIQKIQEFTKADFGYHGDKSFDSLYRPGVMIEQELEQSKNFGVFLYSARVIAD
                     HIRAATFILGDERGIAPSNLEAGYVLRRLIRRAIRHAKIIGISSSFTAQLGKIVIDQF
                     ADEFPSLLENEQLVLAEFTKEEEKFSKTLEKGLREFEKLAADKLDGKDAFILFSTYGF
                     PLEMTQELAEERGLNVDEEKFRQEFKKHQNLSRTASAGKFKGGLADHSTETTRLHTAH
                     HLLLAALQKTLGPHVQQKGSNITAKRLRIDFSHPDKLTDEQLETVQNLVNQAIQNDLA
                     VDWEELPLAEARQRQATGVFEHKYAERVKVYKIYDPTSGEIFSQEICGGPHVKKTSEL
                     GQFSIKKEEASSGGVRRIKAELSKS"
BASE COUNT          444 a          368 c          358 g          340 t
ORIGIN      
        1 gaactggtgg tctcgctccg gcgtgcctga aaaaatgccc ccgggtgaac cgggcggacc
       61 ggacagtgaa atattttggg acttcggcga aaagcttggc catcatgaag cctcgccgtt
      121 tcaagctcaa ccctgtcacg ttaactgtga ctgcggccga ttcttggaaa ttggcaacag
      181 tgtgtttatg gaataccgga aaactaaaac cggctttgag cctctgcctc aaaaaaatgt
      241 tgactttggc ggcggtcttg aacgcatggc cgcggccgcc caaaatgacc cggatatttt
      301 taaaattgac acctttcggc cgctgatcca aaaaattcaa gaatttacta aggctgactt
      361 tgggtaccac ggagataaaa gttttgactc gctttatcgg ccgggcgtta tgattgaaca
      421 ggagttagaa caatcgaaaa actttggcgt atttttatac tcagcccggg tgattgcaga
      481 ccatattcga gcggccacgt ttatcttggg tgatgagcgg ggaatagctc cgtccaacct
      541 tgaggccggc tatgtgctgc gccgattaat tcgccgcgcg attcggcacg ccaaaattat
      601 cggcatctcc agctcgttca cggctcagct cggaaaaatt gtaattgatc aatttgccga
      661 tgaatttccg tcactgctgg aaaacgaaca gttggtccta gccgaattta ccaaagagga
      721 ggaaaaattt tcaaaaaccc ttgaaaaggg cttaagagaa tttgaaaaac tggccgccga
      781 caagcttgac ggtaaagacg cgtttatttt attttcgacc tacggctttc cgctagaaat
      841 gactcaggag ctggctgaag agcgaggact caacgtggac gaagaaaaat ttcgccagga
      901 gtttaaaaaa caccaaaatc tttcccgcac cgcctcggcc ggaaaattta aaggcggctt
      961 agccgatcac tcaaccgaaa caacccggct gcataccgct caccacctgc tcttagccgc
     1021 gctccaaaaa actcttggtc cccacgttca acaaaaaggc tcaaatatca cagccaagcg
     1081 actgagaatt gacttctcgc acccggataa actgaccgac gaacaacttg aaactgttca
     1141 gaatttggtt aaccaagcca ttcaaaatga cttagccgta gactgggagg aactcccgct
     1201 cgccgaagcc aggcagcgcc aggcgaccgg cgtgtttgag cacaaatacg ccgagagagt
     1261 aaaggtctat aaaatctacg atccgacctc aggcgaaata ttctcccaag aaatatgcgg
     1321 cggcccgcac gtcaaaaaaa catcagaatt aggtcaattt agcataaaaa aggaagaggc
     1381 gagtagcgga ggcgtgcgta gaattaaggc tgagttaagc aaatcttgac agttacccgc
     1441 aaatccagta gtataaatat tgacacttta gcaattctta gctagactat tcgcgagctt
     1501 ctaaagacaa
//
LOCUS       DASWHZ010000043         4759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1590948, whole genome shotgun sequence.
ACCESSION   DASWHZ010000043 DASWHZ010000000
VERSION     DASWHZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590948"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VGA49_00970"
     CDS             <1..1254
                     /locus_tag="VGA49_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664372.1"
                     /translation="HYQIEESVLNYDLSFPPELFDNQEFEFAITVNNLSGDNDLKNIK
                     IIPDWPDGFSLLESSVPFQTEIDILPPLEAIIIQAKGRVAGLDQTQKQFAFKGYLRHG
                     QRDLFQRSRELTAPLSYPKFSLTARADQEGYSLGQIIDYQINFSNQEDYDLADVVFSL
                     PLNSDIFNLNSVRSTGVLENGRLVWDLDEFQSVRASQSGSFRFSARLNSKINFSRYFR
                     EQSQIIITPEVSYLTADDGQAPRVKLRGAPAAVKINSDLGLTAFGRYYSAEGDQLGRG
                     PLPPVAGRTTKYWVFLQVVNNLNDVNNLLVTAQLPDTVEFTGRTSVALGEDLSYNPAS
                     RTIQWPVGLIQSFRSGPDNGQGAAFEVAFTPTTDQVGAEPILLTNIAVSGADLFTGVF
                     LQATTGGVTTNLFKDSLAAGKGRVE"
     gene            complement(1424..3217)
                     /locus_tag="VGA49_00975"
     CDS             complement(1424..3217)
                     /locus_tag="VGA49_00975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9664373.1"
                     /translation="MVKKTYSKTKSANAGHRRTQKKSYRKKYTPPAKRHIQTGTQRDH
                     DKLKIMVLGGLEEVGRNMTVLECGDDIMIIDMGVMFPEESMPGIDYVIPNISYLRGKE
                     KNIRGVVITHGHYDHIGAIPHLIGDLGNPIIYTGKLTSGMIQRRQEEFRQAPKLKVVE
                     IDEKSKIKLGKKFEVEFFRVSHTIPDSFGVAVNTPYGYILHSGDFKFDHNPYHEKPID
                     TSRIAGFGKKDVLALMLDSTNAELPGHQISESQVFEEMSKIFAKAEGRLIIGTFASLL
                     SRVQMVMELAEKNNRKVLVEGRSMLQTVDIAHKLGYLKIKPGIIIEAKEANRLPDKKL
                     VVVGTGAQGQQNAFLMRLATDEHRYLSVKKGDTVIFSSSVIPGNERTIQGLKDTLYRK
                     GAKVIHYQMMDVHAGGHAKQEDLKLMIGMIKPKYFIPIEAYHYMLRIHAELAEGVGIP
                     AKNIFVADNGQVIEFDIKGGRLTKRYVPSDYVFVDGLGVGDVSNIVLRDRQVLAGDGM
                     MVVIAAVDARTGRLIGSPDIISRGFVYLKESKKLIEQTRNKVRKILQDKDPKSPPDTL
                     YLKNKLRNEIGSFLYSKTNRRPMVLPVVIAI"
     gene            complement(3229..>4759)
                     /locus_tag="VGA49_00980"
     CDS             complement(3229..>4759)
                     /locus_tag="VGA49_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9664374.1"
                     /translation="YQISEDQTVYTFTLKDNVVWHDQKPLSADDVVFTVKSIQNQEFK
                     SPLLISLRGVKIEKTGDNQISFTLKTPYTPFLSILTFGVLPEHVWGNIPPANSRLAEL
                     NLKPIGSGPWQFKALTRDRQGNLKSYSLEPNENYYGDKPYLKQLVFKFYAGFDEAVQA
                     LANKQIQGISFIPQDYTDQVAKNKNAVIKSLRLPQYTAVFFNRRANVLLKNEKIRQSL
                     AMSIDKNEIINQALKNEGEAIDGPILPGYLGYHLNIKKYSFDLETANRLLDLDGWNKI
                     TPKEYLEAEQKLKAEEESADSDETDEDSSADSGAETSADEPAETGEEIEIDLDQEFFR
                     QKKDSLLKISLTTVDQPEYVKAAEIIKQSWQKIGVRVDLIIIEGTKIQQEVISPRGYQ
                     ALLFGEIIGSDPDPYPFWHSSQTEDPGLNLALFANSQADKLIIEARQTNNLEEREKKY
                     IAFQDILAGEIPAIFLYNPIYTYVIDKTIKGFAISRIILPADRFNGLEQWFIKTKRKL
                     F"
BASE COUNT         1133 a         1054 c         1149 g         1423 t
ORIGIN      
        1 cattatcaaa ttgaagaatc tgtccttaat tatgatttaa gttttccgcc cgagctgttt
       61 gataatcagg agtttgagtt cgccataaca gttaacaatt tatcgggcga taatgatctg
      121 aaaaatatta aaattattcc tgactggccc gacggattca gtctgctgga atcttctgtt
      181 ccttttcaaa cggagattga tattttgccg cctttggaag caatcattat tcaggctaaa
      241 gggcgggtgg ctggtttgga ccagactcaa aaacagtttg cctttaaagg ctatctgcgt
      301 catggccagc gggatttatt tcagcggagc cgggagctga cggctccttt gagttatccc
      361 aaattttctt tgaccgcccg ggctgatcag gaaggttaca gtttgggcca gataattgat
      421 taccagataa attttagcaa tcaggaagat tatgatttgg ctgatgttgt ttttagcctg
      481 cctttaaata gtgatatttt taatttaaat tcagtccggt caactggtgt tttagagaat
      541 ggccggctgg tctgggattt ggatgagttt cagtcggtca gggccagcca gtccggcagt
      601 tttagattca gcgccaggct taattcaaaa ataaatttta gccggtattt cagagaacaa
      661 agtcagatta tcattacccc tgaagtcagc tatctgacag ctgatgatgg ccaggccccg
      721 cgagtcaaac tgagaggggc tccggccgct gttaaaataa attctgatct tggtctgaca
      781 gcttttggcc gttattattc agctgaaggc gaccaactgg gccggggccc gctgccgccg
      841 gtggccggcc ggaccaccaa atactgggtt tttctccagg tggtcaataa tcttaatgat
      901 gttaataatc tgttggtaac ggcccagctt ccggacactg ttgaatttac cggccggacc
      961 agcgtggctc ttggcgaaga cctgtcttat aatccagcca gccggacgat tcagtggccg
     1021 gttggtttga ttcaaagttt tagaagcggc cctgataacg gtcagggcgc ggcttttgaa
     1081 gtggctttta ccccgacaac tgaccaggtt ggcgccgaac cgattttgct gactaatatt
     1141 gccgtcagcg gggctgattt gtttaccgga gtttttcttc aggcgactac cggcggagtg
     1201 acaactaatt tatttaaaga cagcctggcg gccgggaaag gtcgggtaga ataaggcaca
     1261 aggcaatagg caataggcac aagggttggc gtcccgtcag tcgcgactga cgggacaatt
     1321 atttgatggg cataaggtaa aagggttttt actaatgccc gatccccatc taataaatta
     1381 tttctaccct attgcctcgt gcctattgcc ttgtgccttt ttgttagatc gctatcacca
     1441 ccggcagaac catcggccgt cggttagttt tagagtagag aaatgaaccg atttcatttc
     1501 taagcttatt tttcagatac aaagtgtctg gcggtgactt agggtctttg tcctggagaa
     1561 tttttctgac tttatttctg gtttgttcaa tcagtttttt gctttctttc agataaacaa
     1621 agcccctgga gataatatcg ggactgccaa tcagtcgccc tgtcctggcg tcaaccgcgg
     1681 caatgacaac catcattccg tcgccggcca gcacctgccg gtcgcgcaga acaatgttgg
     1741 aaacatcgcc gactcccagg ccatcaacaa aaacataatc tgacggcacg tatcgtttag
     1801 tcagtcggcc gcccttaata tcaaactcaa tcacctggcc gttgtcggcc acaaaaatat
     1861 ttttagccgg aatcccgacg ccttcagcca gttcagcgtg aattcttaac atgtaatgat
     1921 aagcttcaat cggaatgaaa tattttggct tgatcatgcc gatcatcagc ttcaaatctt
     1981 cctgtttggc gtggccgccg gcgtgaacat ccatcatttg ataatgaatc actttggcgc
     2041 ctttgcgata aagggtgtct tttagtccct gaatggttct ttcattgccg ggaataactg
     2101 atgaagaaaa gatcacagtg tcgccttttt taactgacag atagcggtgt tcgtcagtgg
     2161 ctagtctcat cagaaaagcg ttttgctggc cctgggcgcc ggtgccgaca accaccagtt
     2221 ttttgtccgg taggcggttg gcttctttgg cttcaataat aattcccggt ttgattttca
     2281 aatagcccag cttatgggcg atatcaaccg tctggagcat gctccggcct tcaaccagca
     2341 cttttcggtt atttttttca gccagctcca tcaccatctg gacccggctt aagagcgagg
     2401 caaaagtgcc gataatcagg cggccttcgg ctttggcgaa aattttggac atttcttcaa
     2461 aaacctggct ttccgaaatc tgatggccgg gcagttcggc gttggtgctg tccagcatta
     2521 aagccaggac atcttttttg ccaaaaccgg cgatccgcga ggtatcaatc ggtttttcat
     2581 gataagggtt gtggtcaaat ttaaagtcgc cgctgtgcag gatgtagccg tagggggtgt
     2641 tgaccgccac cccaaaactg tccgggatgg tgtggctgac ccggaaaaat tcaacctcaa
     2701 attttttacc gagcttgatt ttggactttt catcaatctc aacaactttt aattttggcg
     2761 cctgccgaaa ttcttcctgg cgccgctgaa tcatgccgga ggtcagttta ccggtataga
     2821 tgatcgggtt gccgaggtcg ccgattaaat gggggatggc tccgatgtgg tcataatgac
     2881 cgtgagtaat aaccacgccc cggatatttt tttctttgcc gcgcagatag ctgatattgg
     2941 gaataacata atcaatcccc ggcattgatt cttccgggaa cattacgccc atgtcaataa
     3001 tcataatatc gtcgccgcat tccagaacag tcatgttccg gccgacttct tccagaccgc
     3061 ccaacaccat gatttttagc ttatcatggt cgcgctgggt tccggtttgg atatgtcttt
     3121 tagccggagg ggtatatttt tttctgtagc tctttttttg agtccggcgg tggccggcgt
     3181 tagctgattt agtttttgag taagtctttt taaccatatg tttagttgtt agaataattt
     3241 tcttttagtt tttataaacc attgttcaag gccgttgaag cggtcggccg gcaggatgat
     3301 ccggctgatg gcaaaacctt tgatagtttt gtcaatgaca taagtataaa ttggattata
     3361 gagaaagatg gccggtattt ccccggctaa aatatcctga aaagcgatat actttttttc
     3421 cctctcctcc aggttgttgg tctgccgggc ttcaatgatt aatttgtcag cctggctgtt
     3481 ggcgaacaag gccaggttca gaccgggatc ttcagtctgc gatgaatgcc agaaaggata
     3541 aggatcgggg tcagacccga tgatttctcc aaagagcaag gcttgatagc cgcgggggct
     3601 gatgacttcc tgctgaattt tggtgccttc aatgataatc aggtcaaccc tgacgccgat
     3661 tttttgccag ctttgtttaa tgatttcagc ggctttaacg tattcgggct gatcaacggt
     3721 tgtcagagag atttttaaaa gggaatcttt tttttgccgg aaaaattcct ggtccagatc
     3781 aatttcaatt tcttccccgg tttcagccgg ctcgtcagct gaagtttcag ccccggaatc
     3841 agcggacgag tcttcatcgg tttcgtcaga atcagccgat tcctcttcag cttttaattt
     3901 ttgttcggcc tccagatatt ctttgggagt aattttgttc cagccatcca aatctaacag
     3961 ccggttggct gtttccaggt caaaagaata ttttttaata ttaaggtgat agccgaggta
     4021 gccgggcaga atcgggccgt caatcgcttc tccttcattc ttcagagcct ggttaataat
     4081 ttcgttttta tcaatgctca tcgccaggct ttgtctgatc ttttcatttt ttaataaaac
     4141 attggcccgc cggttaaaga aaacagcggt gtattggggc agacgcaaag atttgatgac
     4201 agcgtttttg tttttagcga cctggtcagt atagtcctgg ggaataaaac tgatgccctg
     4261 gatttgcttg ttggctaaag cctgaaccgc ttcgtcaaaa ccggcgtaga atttaaaaac
     4321 cagttgcttt aaatacggtt tgtccccgta ataattttca ttcggttcaa ggctgtatga
     4381 ttttaaatta ccctgcctgt cccgggtgag cgctttaaat tgccaggggc ccgagccgat
     4441 cggtttgaga ttcaattcag ccaggcggga attggccggc ggaatattgc cccagacatg
     4501 ttcgggcaat acgccaaaag tcaggattga caaaaaaggc gtgtaggggg tttttaaagt
     4561 aaaggagatt tgattatcgc ctgttttttc aattttgact cctcttagac taatcagcaa
     4621 agggctttta aattcctggt tctgaatgct ttttaccgtg aaaacgacat cgtcggctga
     4681 cagcggtttc tggtcgtgcc agacgacatt gtctttcaga gtaaaagtgt agactgtctg
     4741 atcttcagaa atctgatag
//
LOCUS       DASWHZ010000044         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_842554, whole genome shotgun sequence.
ACCESSION   DASWHZ010000044 DASWHZ010000000
VERSION     DASWHZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842554"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /gene="rplQ"
                     /locus_tag="VGA49_00985"
     CDS             complement(<1..332)
                     /gene="rplQ"
                     /locus_tag="VGA49_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX9664375.1"
                     /translation="MRHRKKKKILDRNKSARQSLLRNLAGSLVLYEKIKTTEAKARAV
                     RSVVEKYITVGKKNDLHSRRQLLKFFYTENQVKKVLEVLGPRYKERPGGYTRIVKLGQ
                     RAGDGAKVV"
     gene            complement(339..1235)
                     /gene="rpoA"
                     /locus_tag="VGA49_00990"
     CDS             complement(339..1235)
                     /gene="rpoA"
                     /locus_tag="VGA49_00990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9664376.1"
                     /translation="MENIPLPNKIEVQPTDNLNRALVVVEPLFEGYGTTLGNALRRVL
                     LSSLPGAAVTGLKIKGVEHEFSAIDNVKEDVIELILNFKQLRLKIFTDDEVRLKLKVS
                     GQKKATAADIEHGSEVEIVNKDMHLATLTSAEADLEIEIFANKGRGYVPTEARDKELL
                     EIGTIAIDSIYTPIVNVGYKVENVRVGQMTNFEKVIYDIETDGTMTPKDAVIQSADIL
                     LDHFAILTDEGIEAAKKAKEALIKKFEEEQKQLEAREATAVEAVDAEKTVADSSAEEE
                     AEEKPKKKRGRPKKSETGEAGS"
     gene            complement(1259..>1651)
                     /gene="rpsD"
                     /locus_tag="VGA49_00995"
     CDS             complement(1259..>1651)
                     /gene="rpsD"
                     /locus_tag="VGA49_00995"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9664377.1"
                     /translation="KKSKRKTGDTSEILMRLLESRLDNVIYRLGYTTSRHLARQIIRH
                     GHFQVNGRLVNIPSYQVKDGELISLDPKSKELKIFKNLSKSLQKQTFPEWVSFDINTL
                     EGKILSKPAVDDLKEAYNINLIIEYYSR"
BASE COUNT          410 a          408 c          295 g          538 t
ORIGIN      
        1 acaactttgg cgccgtcacc ggcccgctgg cccagcttga cgattcgggt atagccgcct
       61 ggccgctctt tatagcgggg tcctaaaacc tccaaaacct ttttaacctg attttccgtg
      121 taaaaaaatt tcaaaagctg ccgcctggaa tgcaaatcat tttttttacc gacagtaata
      181 tatttttcca cgaccgatct gaccgctctg gctttggctt cagtcgtttt aattttttca
      241 tacaatacca aactgccggc caaatttctt aacaacgact gccgggctga tttatttctg
      301 tctaaaattt ttttcttttt gcggtgcctc ataatatttt atgatcctgc ttccccggtt
      361 tcagattttt ttggacggcc tctttttttc ttaggttttt cttcagcctc ttcctctgcg
      421 gatgaatcag ccactgtctt ttcagcatca acagcttcaa cggccgttgc ttcccgggct
      481 tcaagctgct tttgttcttc ctcaaatttc ttaatcagcg cttctttggc ttttttagcg
      541 gcctctatcc cttcatctgt caaaatcgcg aaatggtcca gtaaaatatc agctgactgg
      601 ataaccgcgt ctttcggggt catggtgccg tcagtttcaa tgtcataaat aactttttca
      661 aaattggtca tttggccaac ccggacattt tccaccttat aaccaacatt aacaatcggg
      721 gtgtaaattg aatcaatggc gatcgtgccg atttccagca gttctttgtc cctggcttca
      781 gtcggcacat agccccgccc cttattggca aaaatctcaa tctccaaatc agcctcagcg
      841 ctggtcaaag tcgccagatg catatcctta ttgacaatct caacttcaga tccgtgttca
      901 atgtcagccg ccgtcgcttt tttctgaccg ctgactttta acttcagtct gacttcatcg
      961 tccgtaaaaa ttttcagacg cagctgttta aaatttaaaa tcaattcgat aacatcctct
     1021 ttgacattat caatggctga aaattcatgt tcaacacctt tgattttcaa accggtcacc
     1081 gccgccccgg gcaatgacga cagcagaacc cgccgcaaag cattccctaa agtagtgcca
     1141 taaccctcaa ataagggctc aaccacaacc agagcccggt taagattatc tgtcggctgg
     1201 acttctattt tattaggtaa aggaatgttt tccataaatt tttattttag tctataaatt
     1261 atcttgaata atactcaata atcaaattga tattgtaagc ttccttcaga tcgtcaaccg
     1321 ccggcttgct taaaattttg ccttccagcg tgttgatatc aaatgaaacc cattccggga
     1381 atgtttgctt ttgaagagat ttggataaat ttttaaaaat tttcagttcc ttgctttttg
     1441 gatccagact gatcagctca ccatccttca cctgatatga aggaatattg accagccggc
     1501 cgttaacctg aaaatgaccg tggcggatta tttgcctggc cagatgacgg gaggtcgtat
     1561 agcccagccg ataaataaca ttatcaagtc ttgattccag cagacgcatt aaaatttcac
     1621 tagtgtcgcc ggtttttctt tttgattttt t
//
LOCUS       DASWHZ010000045         8651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_106256, whole genome shotgun sequence.
ACCESSION   DASWHZ010000045 DASWHZ010000000
VERSION     DASWHZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106256"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VGA49_01000"
     CDS             complement(<1..788)
                     /locus_tag="VGA49_01000"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX9664378.1"
                     /translation="MLFGSHVSIAGGLENAPTNASKVGCEVFQMFTRSPYGGKEQPIT
                     KAVAQKFKKACQEHGFKDYYVHAPYYINLASSNNRIRYGSISAIRKELERSDQLGVKY
                     VMAHLGSAKDVGEKEAVKKTAEAIKKILTGYKGKTEFLMEMSAGSGQVIGDTFDELAE
                     ILKLTGKFKNKIGICFDTAHAFASGYDLRTKKAVDDTFKEFDKKIGLKKLKLIHCNDS
                     KTGLGSHVDRHEHLGQGQIGLDGFKAIVKHSKLKKINIILETPK"
     gene            complement(859..2145)
                     /gene="tig"
                     /locus_tag="VGA49_01005"
     CDS             complement(859..2145)
                     /gene="tig"
                     /locus_tag="VGA49_01005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9664379.1"
                     /translation="MEITTNKIDRAQIEFTVEVPFEDLKLFLEKGAERLSEKMKIKGF
                     REGKAPYEVVKREAGEMAIYEEAAQEAIHKTYYQALEQEKLLTVGSPRISVEKLALGN
                     PLVYKATVSLFPEVKVGDYSNLKIKKQPANVTDEKISQTLDHLRKTRAKETLVDRPVK
                     DGDRVEIDFDVFVDNIPIDNGAHKKYPLIIGQGSMIPGFEEQLVGLAKDQTKEFKLPF
                     PKDYHAKNLAGKEADFKVKLLAVYQIDLPELNDQFAKTLGQFNSLPDLKRQIKQNLEA
                     EEKMKIDHDSEVEMLEKLMELSRFGDLPDVLVNNEINRMVHELKGNIEAQGLKWDDYL
                     NSLKKKEEDLKLELTPGAIKRVKVSLMTHQIARDQKIEADSAEVEAEIKKQREIYKDQ
                     PQILENLSQAGYRDYLRNVMINKKVIQFLKEKIIVE"
     gene            complement(2229..2579)
                     /locus_tag="VGA49_01010"
     CDS             complement(2229..2579)
                     /locus_tag="VGA49_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014166634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9664380.1"
                     /translation="MGIESYQDLVVWQKGIELSVKIYELTKSFFKSELYGLTSQMRRA
                     SVSIPSNIAEGYCQHSRKSYLKYIYRSLGSVAELETQIIIAKRAKLIKESEDIDQLIK
                     EIFRMLLALIKKLK"
     gene            complement(2684..3154)
                     /locus_tag="VGA49_01015"
     CDS             complement(2684..3154)
                     /locus_tag="VGA49_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664381.1"
                     /translation="MKKYTPYIFSGLIILIFIILTILLLNIPINEPPTLLERVSGRIL
                     IQVMDEDQVWYIYPGRNEVSNGVYPKDQKRYLLAENGFEVLKSLAVKLENIDEEKKYP
                     GHIVTYDQREYIYLDPVESKKYKFNDQQELLEVIEQVGLGAYNRDLKRIESGVE"
     gene            3247..3432
                     /locus_tag="VGA49_01020"
     CDS             3247..3432
                     /locus_tag="VGA49_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664382.1"
                     /translation="MEQKTALSDEKIMRLAAAGFAIIFMILLAGLLLSIHLTEKQAEG
                     AKQPGSPTEQGGRSDGK"
     gene            3422..3625
                     /locus_tag="VGA49_01025"
     CDS             3422..3625
                     /locus_tag="VGA49_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664383.1"
                     /translation="MENDDENEEGNPNEGLFFGCLREAVITTIVAVVIAFLVYAIREK
                     IHQNPEAVRTVPTSPRGGGSDGK"
     gene            3615..3791
                     /locus_tag="VGA49_01030"
     CDS             3615..3791
                     /locus_tag="VGA49_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664384.1"
                     /translation="MENEQGKTALSDEMMVKLATIGIIILMLLIAVALTIYFGNPEKP
                     ADKQQTPPTRPEGK"
     gene            3792..4355
                     /locus_tag="VGA49_01035"
     CDS             3792..4355
                     /locus_tag="VGA49_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664385.1"
                     /translation="MEPTEKADNAPRINLGFKVLPFVVGLIVTFIVAGLGTLALKHYW
                     PEVYSDQKENPAEKTAADATPIIQLVEQPEKTSWLEIKKVINRKFHDSDFQSVEITWQ
                     ENQAVEHLSVILRGSRVELNLHLLETPPVVYDVRSVLGLPQDALRLWAGRKLQSLIDQ
                     NMFDDFPREDIKRLVLFRISLLTTKKF"
     gene            complement(4478..6463)
                     /locus_tag="VGA49_01040"
     CDS             complement(4478..6463)
                     /locus_tag="VGA49_01040"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9664386.1"
                     /translation="MSGVFTLVLASALIAIIYGLILIKMIMKLSPGDERMTEIAGAIQ
                     QGAKAYLNRQYKTIAIIAVVLFVLLWIFIGLKTAIGFLIGAVFSALAGYIGMNVSVRA
                     NVRTAQAAKEGLSQALKVSVQGGAVTGFLVVGLALLGVAGYFLITKDVQALIGLGFGG
                     SLISIFARLGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMA
                     ADLFETYAVTAVATILLGSLMFPGFANAVYYPLVLGAIAILTSIVATFFIRLPKSNNI
                     MGALYMGLIVAGVLAAIVFYPITQWLMAGNGLYSVLNIYIASLIGLVVTALLVIITDY
                     YTSTKYSPVRKIAKASETGHGTNIIAGLALSMKSTALPIVVIAAGIWGAYALAGVYGI
                     AIAAMAMLSLAGIIVAIDAYGPITDNAGGIAEMADLPQEVRVVTDALDAVGNTTKAVT
                     KGYAIGSAALAALVLFADYTHALGAGFEFMIDNPKVLIGLFIGGLLPYFFGALSMEAV
                     GKAAGKVVEDVRQQFKEKPGIMERTEKPDYGRTVDIVTKSAIREMIIPSLIPVLAPIL
                     VGFILGVQALGGLLVGSIITGIFVAVSMTTGGAAWDNAKKYIEEGHHGGKGSEAHKAA
                     VTGDTVGDPYKDTAGPAINPMIKILNIVALLIVALLI"
     gene            complement(6652..7890)
                     /gene="nusA"
                     /locus_tag="VGA49_01045"
     CDS             complement(6652..7890)
                     /gene="nusA"
                     /locus_tag="VGA49_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX9664387.1"
                     /translation="MSKKSPIYEAIKLICDEKGLSMETVVETIESALAAAYRKDFGEP
                     NQNIKVKFDPETGQSEVFDVKTVVADMTEEEIEAQRELTAEELEEQGIKRYNPKTDLM
                     ISEAKNIKKDAELEEEIITKLEVPADYGRMAAQTAKQVITQKLREAERKTIFEEFKEK
                     EGELIVGVVQRQEGRVFLVDLGRAIGVLPPDQQVHSERYKSGQRIKVYVLSVEMGGKG
                     PEITLSRAHEDIVKKLFGLEIPEIASGAIEIMSVAREAGSRTKISVRAVEENIDPIGS
                     CVGQRGTRVQTIISELGGEKIDIIEYDGDSAAYITNALSPAKVIDLDINDQEKLAVVT
                     VAEDQLSLAIGKAGQNVRLAAKLTGWKINIKAEEGEKVISSEDEESQQPAVNSQQEEK
                     QEKAPEEEKKKEPTDPDVKN"
     gene            complement(7974..8333)
                     /locus_tag="VGA49_01050"
     CDS             complement(7974..8333)
                     /locus_tag="VGA49_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX9664388.1"
                     /translation="MNIPKGKLGEEVAAKYLRQRDYRIIEQNYYGKYGEIDIIAEKDN
                     ELIFIEVKTRFNIKFGYPEESFNKKKRISLIKTIQKYLFLKNLEMDYRIDLIAVIINI
                     KGQKVQIKHFENVIIDI"
     gene            complement(8393..>8651)
                     /locus_tag="VGA49_01055"
     CDS             complement(8393..>8651)
                     /locus_tag="VGA49_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664389.1"
                     /translation="RKKDVALKRFSRKLMIFSFTMAVIGAVVLFINFEQLPLLSNRFL
                     FLLWGLGGLVWLGFILKYLFVNVPRLKKQLGEKQRLEKYLP"
BASE COUNT         2376 a         2094 c         1679 g         2502 t
ORIGIN      
        1 tttttcggtg tttctaaaat gatattaatt tttttcagtt tagaatgttt gacaatggct
       61 ttaaacccat ctaagccaat ctggccctga cccaaatgct cgtgccggtc aacatgggaa
      121 cctaaccctg ttttggaatc attgcagtga atcaatttca attttttcag accgatcttt
      181 ttatcaaatt ctttgaaagt gtcatcaacc gcttttttgg ttcttaaatc gtagccagag
      241 gcaaaagcgt gggccgtgtc aaagcagatt ccaattttat ttttaaactt gccggtcagt
      301 tttaaaattt cagccagctc atcaaaggta tcgccaatca cctggccgga accggctgac
      361 atttccatta aaaattcagt tttcccttta tagccggtta aaattttttt aatggcttca
      421 gcggtttttt taacggcctc tttttcacca acatctttgg cgctgcccag atgagccatg
      481 acatatttta ctcccagctg atcgctccgc tctaactctt ttctaatggc ggaaattgat
      541 ccgtatctga ttctattatt agaagaagct aaattgatat aataaggagc gtgaacataa
      601 tagtccttaa agccgtgttc ctgacaagcc tttttaaatt tttgggcgac cgctttggta
      661 ataggctgtt ctttgccgcc ataaggcgac cgggtaaaca tctgaaaaac ctcgcaacca
      721 acctttgaag cgttagttgg cgcattttcg agaccgccag caattgagac gtgagagccg
      781 aataacatct ttataaaatt tctaatttct aatatctaat ttctaaacaa tttctaatga
      841 tccaatacac aaatcctatt actcaactat aatcttttct ttcaaaaatt gaatgacctt
      901 cttgttaatc ataacatttc tcaaataatc ccggtagccg gcttgcgaca ggttttccag
      961 aatttgcggc tgatctttgt aaatttcccg ctgttttttt atttctgctt caacttcggc
     1021 tgaatcagct tctatttttt ggtcccgggc gatctgatgg gtcattaaag aaactttgac
     1081 ccgcttaatg gcgcccggcg tcagctccag cttcaagtct tcctctttct tttttaaact
     1141 gttcagataa tcgtcccatt tcaacccctg ggcctcaata tttcctttga gttcgtgaac
     1201 catccggttg atttcattat taaccagaac atcgggcaga tcgccaaacc tggacaactc
     1261 cattaatttt tccagcatct ccacttcaga gtcatggtca attttcattt tttcttcagc
     1321 ttccagattt tgtttaattt gtctttttaa atctggcagg ctgttgaatt gccccagagt
     1381 tttggcgaac tgatcgttca gttccggcaa atcaatctga taaaccgcca gtagtttaac
     1441 tttaaaatca gcctctttgc cggccaaatt tttagcgtga taatctttgg gaaagggcag
     1501 tttaaattct ttggtttgat ctttggccag accgaccaac tgttcttcaa agccggggat
     1561 catacttccc tggccgataa tcaacgggta ttttttatga gcgccgttgt caattggaat
     1621 attgtcaaca aaaacatcaa aatcaatttc cacccggtca ccgtctttaa ccggccggtc
     1681 aactagagtt tctttggccc gcgtttttcg caaatgatcc aaagtctgac tgattttttc
     1741 atcagttaca ttggccggct gttttttaat tttcaaattt gaatagtcgc cgactttgac
     1801 ttccggaaac agagaaacag tcgctttgta aaccaaagga ttacccaaag ccagtttttc
     1861 aactgaaatc cggggcgagc cgacagtcaa aagtttttcc tgttccaaag cctggtaata
     1921 agttttatga atcgcttcct gggcggcttc ttcgtaaata gccatttccc cggcttctct
     1981 tttaaccacc tcgtagggag ctttgccttc acgaaagcct ttgatcttca ttttttcaga
     2041 caaccgttca gcgccttttt ccagaaaaag ttttaagtct tcaaacggaa cttctactgt
     2101 aaactctatc tgtgctctgt ctatcttgtt agtagtgatt tccattttat tgaaaattaa
     2161 tagtatatag gcaacaggca gagggcaaca ggcaatagtc agggatcagg caaaaaagga
     2221 ttagtagttt attttaattt tttaattaaa gctaaaagca tcctaaaaat ctcttttatc
     2281 aactgatcaa tgtcttcgct ttctttaatt agcttcgctc ttttggcaat gattatttga
     2341 gtttccaatt cagcaactga tcctaaagat ctgtaaatat atttcaaata acttttcctt
     2401 gaatgttggc aatacccttc ggcaatgtta gacggaattg acacagaagc ccttcgcatc
     2461 tgtgacgtta atccatataa ttcagacttg aaaaaagatt tagttaattc atatatcttg
     2521 actgaaagct caatcccttt ctgccaaaca accaaatcct gatacgactc aatacccata
     2581 catttctcct ttctcccttg ttgcctgaac ccctttgcct agcctgttgc ccattgcccg
     2641 ttgcctgttg cctttatcaa acaacctaca ataaaaattc aaattattca acccctgact
     2701 caattcgctt cagatctcta ttatacgctc ccaaaccaac ctgctcaatc acttctagta
     2761 attcttgctg atcattaaac ttatatttct tgctttcaac cggatccaaa taaatatatt
     2821 cccgctgatc ataagttaca atatgtccgg gatatttttt ctcctcatca atattttcta
     2881 atttaaccgc caaacttttc aaaacttcaa aaccattttc agccaagaga tacctttttt
     2941 gatccttggg atataccccg ttagaaactt cgtttctacc ggggtaaata taccaaacct
     3001 gatcttcatc catcacctga atcaaaatcc gcccgctgac ccgttccagc aaagtcggcg
     3061 gctcgttaat cggaatattt aaaagtaaaa tagtcaggat aataaatatc aaaatgatca
     3121 ggccgctaaa aatgtaaggg gtgtattttt tcatagtctg ttattttaac ataaaaacaa
     3181 ttgacacaag tagataattt tgttagttta gtatgatccc gtactttaac aaccaggagg
     3241 tgtccgatgg aacaaaaaac ggccttgtcc gatgaaaaaa taatgaggct ggcagccgcc
     3301 ggcttcgcga tcatcttcat gattctgctg gccgggctgt tactgtcaat tcacttgacg
     3361 gaaaaacaag cggagggcgc taaacagccg ggttctccca ctgaacaagg aggccgatcc
     3421 gatggaaaat gacgacgaaa acgaggaagg caatccgaac gaaggcttgt tctttggctg
     3481 tttacgcgag gcggtcatca ccaccatcgt ggccgtggta atcgcttttt tggtctatgc
     3541 catccgcgaa aaaattcatc aaaatcccga ggcggtcaga acagttccaa ccagcccgcg
     3601 aggaggtgga tccgatggaa aatgaacagg gaaaaacagc cttatcagac gaaatgatgg
     3661 tcaaactggc gacgatcggc attatcatcc tgatgctgtt gatcgcggtc gcgctgacga
     3721 tctactttgg caacccggaa aaaccagcgg acaaacagca aactccgcca acacgaccgg
     3781 aaggaaaata aatggaacca acagaaaaag cagacaacgc tccccggatc aacctgggct
     3841 tcaaggttct gccctttgtc gtgggactga tcgtcacttt cattgtggcc ggcctgggaa
     3901 ctctggcgct gaaacattac tggccggaag tttactccga ccaaaaagaa aatcctgccg
     3961 aaaaaacggc cgccgacgcg accccaatca ttcagctcgt tgaacagcca gaaaaaacca
     4021 gctggctgga gatcaaaaag gtcatcaacc gaaaatttca tgacagcgac ttccagtcag
     4081 tggaaatcac ctggcaggaa aatcaggctg tagagcattt gtcagtcatc ctgcggggat
     4141 ccagggtgga actaaatctt catcttttgg aaaccccgcc ggtcgtctat gacgtcagga
     4201 gtgtcctggg cctgccccag gatgctttaa ggctctgggc gggcaggaag cttcaaagct
     4261 tgattgacca gaatatgttt gatgactttc cgcgcgaaga catcaaacgg ctggtactgt
     4321 tcaggatcag cctgctgaca acaaaaaagt tttaaggagg accagactcg gcaacgggtc
     4381 ttttttttga agcgaataaa tagctaatct aaacaaatta aaaagcgaat agcagctaat
     4441 ctcgactaat cgctttttta ttcgcaaaaa ttagaattta tatcaacaaa gccacgatca
     4501 gcaacgccac gatatttaga atctttatca ttggattgat cgccgggccg gccgtgtctt
     4561 tatagggatc gccgacggtg tcgccggtca cggccgcttt gtgggcttcc gatcctttgc
     4621 cgccgtgatg gccttcttca atatattttt tggcgttgtc ccaggccgcc ccgccggtcg
     4681 tcattgagac ggccacgaat atcccggtga taattgagcc aaccagcaag ccgcccaaag
     4741 cctgaacgcc caggataaag ccgactaaaa ttggcgccag aaccggaatt aatgacggaa
     4801 taatcatttc tctgatggcc gacttagtaa caatatcaac cgtccggccg tagtccggtt
     4861 tttcagtccg ctccataatg cccggttttt ctttaaactg ctgacggaca tcttccacca
     4921 ctttgccggc cgctttgccg accgcttcca ttgataaagc tccaaaaaag tagggaagca
     4981 ggccgccgat aaataacccg attaaaactt tggggttatc aatcataaat tcaaaacccg
     5041 cgcccaaagc gtgggtatag tccgcgaaca aaaccaaagc ggccagagcg gctgatccaa
     5101 tggcgtagcc tttggtgact gccttggtgg tgttgccaac cgcgtccaga gcgtcagtca
     5161 ccacccgaac ctcctggggc aaatcggcca tctcggcgat gccgccggcg ttgtcagtga
     5221 tcgggccgta agcgtcaatg gcgacgatga tgccggccaa agacagcatg gccatggcgg
     5281 caatggcaat accgtaaaca ccggccaaag cgtaagcccc ccagatgccg gcggcaatca
     5341 ccacaattgg cagggcggtt gatttcattg agagcgccag cccggcgatg atattggtcc
     5401 catggccggt ctcggacgct ttggcgattt ttctgaccgg gctgtattta gttgaagtgt
     5461 agtaatcagt aatgattacc agcagggcgg tcacgactaa gccaattaat gaagctatat
     5521 aaatattcaa aactgaatac aggccattac cagccatcag ccattgggta atcggataaa
     5581 aaacaatcgc cgccaaaacg ccggcgacaa tcagccccat gtacagggcg cccataatat
     5641 tgtttgattt gggcaatcta ataaaaaaag tagcgacaat tgaagtcaaa atagcgatcg
     5701 ctcccaaaac caacggataa taaacggcgt tagcaaaccc tggaaacatt aaagagccta
     5761 aaagaatggt cgccacggcc gtcacggcgt aagtttcaaa cagatcagcc gccatgccgg
     5821 cgcagtcgcc gacattatcc ccgacattgt cggcaatgac cgccggattc cgcggatcgt
     5881 cctcgggaat gccggcttcc actttgccga ccaggtcagc tccgacatca gccgctttgg
     5941 tgtaaatccc gccgcccagc cgggcaaaaa tagaaatcaa actgccgcca aagccaagac
     6001 caatcagggc ctgaacatct ttggtaatta aaaaataacc ggcgactccg agcaaagcca
     6061 ggccgacgac cagaaagcca gtcaccgccc cgccctgaac cgacactttc agagcctggc
     6121 tcaagccttc tttggccgcc tgagccgtcc ggacattggc ccggactgaa acattcattc
     6181 caatatagcc ggccaaagcg ctgaaaaccg ccccaatcaa aaagccaatg gccgttttca
     6241 gaccaataaa aatccagagt aaaacaaata aaactacggc gataatggca atagttttat
     6301 attgacgatt caaataagct ttggcgcctt gctgaatggc gccggcgatt tcagtcattc
     6361 tttcatcgcc agggcttagt ttcatgatca ttttgatcaa aatcaagccg tagatgatgg
     6421 cgataagggc gctggcgaga acgagggtaa agacacctga catatcattt tgaattaatt
     6481 aattattgga ttgcgaatga cgattgttat aagtaaaaag taagaagtaa aaagtaataa
     6541 gttcaagtaa taagttagaa gttgtttaaa ataaataatt ccaaattttt tactttttac
     6601 ttcttacttt ttacttaatt tatttcgtaa tccgtaatta taaatagtct tttaattttt
     6661 cacgtcggga tcagtcggtt ctttcttttt ttcttcttcc ggagcctttt cttgcttttc
     6721 ttcctgttgg ctgttgactg ctggttgttg actctcctca tcttccgaac taataacttt
     6781 ttcgccttct tcagctttaa tgttgatctt ccagccggtc agtttggcgg ccagccggac
     6841 attttgaccg gccttgccaa tggctaatga cagctggtct tcagcgaccg tcaccacggc
     6901 cagtttttcc tgatcattaa tatcaagatc aatcactttg gccggcgata aagcgttggt
     6961 aatataagcg gccgaatctc catcatattc aatgatgtca attttctcgc cgcccagctc
     7021 gctaataatg gtctggaccc gggtgccgcg ctggccaacg caggagccga ttggatcaat
     7081 attttcctca accgccctga ccgagatctt ggtccgcgac ccggcttccc gggcgactga
     7141 cataatctct atggcgccgc tggcgatttc cggaatttcc aggccgaata attttttgac
     7201 aatatcttca tgagcccggg acaaagtaat ttccggtccc ttgccgccca tctccacgct
     7261 caaaacataa actttaatcc gttggccgct tttgtagcgc tcactatgca cctgctgatc
     7321 cggcggcaaa acaccgatcg cccggcccaa atcaaccaga aaaacccggc cttcttgtcg
     7381 ctggacaacg ccaacgatta gctcgccttc tttttcttta aattcttcaa aaatagtttt
     7441 tctttcagcc tcccgcaatt tttgggtgat aacctgcttg gcggtctggg cggccatccg
     7501 cccgtagtca gccggcactt ccagtttggt aataatttct tcttccagct cagcgtcttt
     7561 tttaatgttt ttagcttctg aaatcattaa atcagttttg ggattataac gtttaatgcc
     7621 ctgttcttcc agttcttcgg ccgtcaactc ccgctgggct tcaatttctt cttcagtcat
     7681 gtcagccact actgttttca cgtcaaaaac ttcggactgg ccggtttccg gatcaaattt
     7741 aactttaata ttttgattag gttcgccaaa atctttgcgg taggcggccg ccagggcgga
     7801 ttcaatcgtc tccacgactg tctccattga caggcccttt tcatcgcaaa ttaatttaat
     7861 ggcttcgtag attggtgatt ttttagacat aaagatggtt ttttataaaa acagccagtt
     7921 ctattctgaa ccagctttaa atcatagtag catatatccg ggaaataatc aaattaaata
     7981 tcaataatta cattttcaaa atgttttatc tggacttttt gtcccttgat attgataata
     8041 actgctatta aatctattct ataatccatc tccaaatttt ttaaaaataa atatttctga
     8101 atagttttaa taagacttat tcgcttcttc ttgttaaagg actcttctgg ataaccaaat
     8161 tttatattaa agcgagtttt aacttcaata aaaataagtt cattatcttt ttctgcaatt
     8221 atatctattt caccatattt accataataa ttttgttcaa taattctata atctctttgt
     8281 ctcaaatatt tggctgccac ctcttcccct aattttccct ttggtatatt catataacaa
     8341 aaacacgccc tttctctggc atgctttata ttaaatgcta tttaattaat ccctacggca
     8401 aatatttctc caacctttgc ttctctccca gctgtttctt caaccgggga acgtttacaa
     8461 acagatattt caaaatgaaa ccgagccaaa ccagtccgcc caacccccac agtaaaaata
     8521 aaaaccggtt tgacagtaaa ggcagctgct caaaatttat aaacaacacc accgctccga
     8581 taaccgccat tgtaaaacta aaaatcatta acttcctgga aaatcttttc agggcgacat
     8641 ccttttttct g
//
LOCUS       DASWHZ010000046         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_851726, whole genome shotgun sequence.
ACCESSION   DASWHZ010000046 DASWHZ010000000
VERSION     DASWHZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851726"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGA49_01060"
     CDS             complement(<1..670)
                     /locus_tag="VGA49_01060"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664390.1"
                     /translation="MFFNQRTKISLCLLITSLLLVLFFIPPVMAQNDEEPSGLLQEVS
                     SPLGDAATRAGFQTGGEAVLETTTGRIIGVFLGFLGVIALVLVLYAGFLWMTAGGNEE
                     KVTKARKLMTSAVIGLIIILISGFITVFIMSKLQAPGRASLNAPLALIQPALAQDDGE
                     EEPTPSSLERVLGESGLVGGDIERGKYEGELGFIIGQIISIFLGFLGVVALVLVLYGG
                     FMWMT"
     gene            complement(795..1229)
                     /locus_tag="VGA49_01065"
     CDS             complement(795..1229)
                     /locus_tag="VGA49_01065"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664391.1"
                     /translation="MIKKNRKILRSAILSFLFLLLVNGMSVNVLNAQNDQPFIDLGGS
                     KIGEIRERLRLTGDEAEFGTQTDLSQIVGAIIQTFLGFLGVVALILIIYGGYMWMTAG
                     GNEEKVKKAQTLLRNAVIGLIIISLAYAITAFVIQRIVGAAV"
     gene            complement(1235..1714)
                     /locus_tag="VGA49_01070"
     CDS             complement(1235..1714)
                     /locus_tag="VGA49_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX9664392.1"
                     /translation="MARPKSFKLKKIITGAVVLVLLLLLVLPALNVNAQAESVIDRAE
                     REANEVIEAGGGGPVDVIGELTNAATKGGVPITDQPEIVVGRLVKGFLGVLGMVALIL
                     VIYGGFLIMTGSKGGKEGDINKGKDVLKWAIIGLIIIFSSYAIADFVVDRALRAVGA"
     gene            complement(1792..2238)
                     /locus_tag="VGA49_01075"
     CDS             complement(1792..2238)
                     /locus_tag="VGA49_01075"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX9664393.1"
                     /translation="MKNLISKVFSNPFDKLRVGLSKPKAKKAVLGLVSLCFVFSIAVV
                     PMLVFAQEDIFGVEYGEALGLGKKDPRETIALIIRVFMGFLGTIAVIIVLLGGFKWMV
                     AGGNEEKVGEAKKLLLAGVIGLVIILSAYAITNFVLTQLISVTNAG"
     gene            complement(2509..>2854)
                     /locus_tag="VGA49_01080"
     CDS             complement(2509..>2854)
                     /locus_tag="VGA49_01080"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664394.1"
                     /translation="LLSSLLMIIVVLPALAKTGETTKGTVVSLEDPLGIKEGGPAELY
                     GRIIRAFLQVIGMVTLLIAIIGGYWLLTSAGNPERVKKGKDTFFWGAVGLVGTLASYV
                     IVRYIIQALVGT"
BASE COUNT          855 a          776 c          484 g          739 t
ORIGIN      
        1 cggtcatcca cataaagccg ccgtacaaaa ctaaaaccaa agcaactact cccaaaaatc
       61 ccagaaatat gctaataatt tgcccgatta taaatcccag ctctccctca tatttacccc
      121 gctctatatc ccctcccacc aggccgcttt cacccaaaac tctctccagg cttgagggcg
      181 tcggctcttc ttcgccgtca tcctgggcca gggccggctg gattaaggcc agcggggcgt
      241 tcaagctggc ccgcccggga gcttgcagtt ttgacatgat aaaaacggtg ataaaaccag
      301 atattaaaat aataatcaaa ccgatcaccg ctgaagtcat taacttcctg gctttggtca
      361 ctttttcctc attgccgccg gcggtcatcc ataaaaaccc ggcgtagagc actaaaacca
      421 aagcaatcac tcccagaaat cccaaaaaaa ctccaatgat ccggccggtg gttgtttcca
      481 aaacagcttc gcctccagtt tgaaacccgg cccttgtggc cgcgtcaccc agcggactgg
      541 aaacttcctg taataaaccg ctgggctcct cgtcattttg agccataacc ggagggataa
      601 aaaaaagaac cagcaaaagc gaggtaatca gcagacataa acttattttt gttctttggt
      661 tgaaaaacat ttttggtaat ttctaatttc taattaacct aatttctaat caatatccaa
      721 ttactaatgt ctaatgattt aaaattggat cctgggattt tattagtaat tagaaattaa
      781 aaattagaaa ttttctaaac cgccgccccg acgattctct gaataacaaa cgccgtaatg
      841 gcgtaagcta aactaataat gatcagccca atcacggcgt ttctcaataa tgtttgggct
      901 ttttttactt tttcctcgtt gccgccggcc gtcatccaca tatagccgcc gtaaattatt
      961 aaaatcaaag cgaccacacc caaaaatccc agaaaagtct gaataatcgc gccgactatt
     1021 tgtgataaat ctgtctgcgt gccaaactca gcttcatcgc cagttaatct taagcgttcc
     1081 ctaatttcac ctatttttga accgcctaga tctataaatg gctgatcgtt ttgtgcattc
     1141 aaaacattta ccgacattcc attcaccagt aacagaaaca gaaaacttag aatagctgat
     1201 cttaaaattt ttctattttt tttaatcatt tattttaagc cccaaccgcc ctcaaagccc
     1261 ggtcaacgac aaaatcagcg atggcgtaag acgaaaaaat aattattaaa ccaataatcg
     1321 cccatttcaa aacatctttg cctttattga tgtccccttc tttccctccc tttgagccgg
     1381 tcataattaa aaacccgccg taaatcacta aaatcaaagc aaccatcccc aaaactccca
     1441 aaaatccttt gactaagcgg ccaacgacaa tttccggctg atctgtaatc ggcactccgc
     1501 cctttgtggc cgcgttggtt aattccccaa taacatcaac tggcccacct cctccggcct
     1561 caattacttc attagcctct ctttccgctc ggtcaattac tgactctgcc tgagcattaa
     1621 catttaaggc tggcaaaacc aaaaggagca gcagtaccaa aaccaccgcc cctgttataa
     1681 tctttttcaa tttaaagctt ttaggtctag ccatgctaat acttaaatct tatccgccaa
     1741 aggcggatcc acctctggtg gacaacttta accttacaac ttaaatctgc cctagcccgc
     1801 gttagtgacc gaaatcaact gcgtcagcac gaaattggta atggcgtaag ccgacaagat
     1861 aatcaccagc ccgatcactc cggccagcaa taattttttg gcttcgccga ctttttcctc
     1921 attgccgccg gcaaccatcc atttgaaacc gcccaacaaa acaatgatca cggcaattgt
     1981 tcccaaaaag cccatgaaaa cccggataat cagagcgatt gtttcccgcg ggtctttttt
     2041 gcccagaccc agagcttcac cgtactcaac cccgaaaata tcttcctggg caaaaaccag
     2101 cattggaacc acggcgatgc tgaaaacaaa gcacaggctg accaggccca gcactgcctt
     2161 tttggccttc ggcttactca ggccaaccct gagtttatcg aatgggttgg aaaaaacttt
     2221 tgaaataaga tttttcatgt ttactcccct tataaggaaa ggcaaattat ttgcgtcttt
     2281 cctgttaaat tattttaata aagttgacta attattaaat tataatatct ttcccctaca
     2341 accgtcaagc aagttatcca cttcgaattt gctcaggcac agccttcaca aattctcagt
     2401 gtaaacactc acgaaacccc ctcactgtta aaaactcccc acagggcaaa ttcgaagtgc
     2461 cctgaggaaa ttttgagagc tttgctcgaa aaaatgacga agggcaagct acgtaccgac
     2521 caaagcctga ataatgtagc ggacaataac ataactggcc aaagtgccaa ccaaaccgac
     2581 cgctccccag aaaaaagtgt ctttcccttt tttgactctt tccggattgc cggccgaagt
     2641 taacagccag taaccgccaa taatggcgat taacaaagta accatgccaa ttacctgtaa
     2701 aaaggcccga attatccggc cgtaaagctc ggccggcccg ccctctttaa tccccaaagg
     2761 gtcttccaat gacacgaccg tccctttagt tgtctcgcca gttttagcca gagccggcaa
     2821 aacaactatt atcatcagca agcttgataa caga
//
LOCUS       DASWHZ010000047         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1220408, whole genome shotgun sequence.
ACCESSION   DASWHZ010000047 DASWHZ010000000
VERSION     DASWHZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220408"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="der"
                     /locus_tag="VGA49_01085"
     CDS             complement(<1..821)
                     /gene="der"
                     /locus_tag="VGA49_01085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9664395.1"
                     /translation="MELPQLQNVAIVGRTNVGKSTLFNRLTEEQKAIVSKVPGTTRDP
                     NIGFCLWRGYYFNLIDTGGLDVSKKEKIEKNIIEQAELAIKKADLILLLIDIRAGLLI
                     QDKDLIRKMKQSKKPFLLVANKADGPKLRKKVEQTNFLGVKNVWPASAVTGAGTGDLL
                     DEVVSQLSRLPSFKKVTQVIPEILFKIAIIGKPNVGKSSILNAILGKEKVIVSEIPHT
                     TRETNDTLISYKNKLILLIDTAGIRKKAKISDKLEKESVARSLRAMKSADLTLFV"
     gene            complement(981..2531)
                     /locus_tag="VGA49_01090"
     CDS             complement(981..2531)
                     /locus_tag="VGA49_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX9664396.1"
                     /translation="MIKNPKIDLLDPQTRESKDFKEKNPWLKLAKFSFYLVAVLGILF
                     FFFSSRIIFPSDESVFANIKSFSQNYLFDQLKQMTGSSHLLRGEENDRINFLLLGIGG
                     KGHQGPNLTDTIILGSYKPSSGQLALISIPRDLVVPLPGYGWRKINTLNVFGGIDNDG
                     QGAKFTADFISQLLDLPIQYHFLIDFSGFEELIDTVGGVEIFVENSLNDYQYPVKGKA
                     NATEEARYEHLYIEQGLQEMDGKLALKYARSRHALPPENSDFARSRRQQKLLLAVKDK
                     VLSYKILLSPNKILKILNTLEDNIQTNIKLSDISRMMEIARDFDNNRIINQVLDNGPD
                     GPLVANMYDGASVLEPRADDFSEIKSIVSNIFDQTFVYTMENKTKTLLGEFSPKTSEI
                     DPAEAAETDKQAEPELEIITQDSLPVTVVTKKEEATIEILNGTWITGLAAKTELKLVN
                     DGYDVINIGNAQTHDYEKTVIYSLNNNQFPKTTDYLKKEITSNLLTSGNQEISQADFL
                     IILGRDAN"
     gene            2636..3226
                     /gene="lepB"
                     /locus_tag="VGA49_01095"
     CDS             2636..3226
                     /gene="lepB"
                     /locus_tag="VGA49_01095"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9664397.1"
                     /translation="MKNKKPAYLFESKTVFLKSSAEFILELVKIVIISLAIIIPVRYF
                     LIQPFYVKGASMEPNFFDHEYLVIDEISYRFKEPQRGEIVVFKYPRDPSQYFIKRIIG
                     LPGDKVEIKDGRVYVGNLKLDESAYLNPETKTLGDLVITLEGDEYFLLGDNRNFSLDS
                     RSFGPVKKSFIIGRTWFRGWPFSKIMVFEKPLYNIE"
     gene            3295..4617
                     /gene="hisS"
                     /locus_tag="VGA49_01100"
     CDS             3295..4617
                     /gene="hisS"
                     /locus_tag="VGA49_01100"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9664398.1"
                     /translation="MPRKKEEPKEELVVSVRKSPKLVRGMRDVLPQDQKYWAYIYNKI
                     DELTGTYQFEKIETPILEETALYVRGIGKKSDIVEKEMFSFVDQGGSNLSLRPEATAG
                     VVRAYINHGMHNLPQPVRLYLTGTFNRHERPQAGRLREFHQFDCEALGDDSPVLDAQV
                     ILIGFNLIKELGLPVQVQINSLGCDTCRPEYIEKLSSYYKIHKKKICEDCQKRLTKNP
                     LRLLDCKNEQCQEVKLEAPQIVDWLCADCREHFIKVLEYLDELEVAYHLNPQIVRGLD
                     YYNRTTFEYFLEDEEDTKTALGGGGRYDGLVELLGGPRETPAVGMAIGLERLIAKIRD
                     KNIPVPEKDTPDIFLAQLGEKARKKSLILFEKLRKAGFKVAESFSKDNLKNQLETANK
                     LGVKFTLVLGQKEILDGTILIRDMEGGIQEVVDYNKIEHEINKRLSKV"
BASE COUNT         1309 a          891 c         1079 g         1415 t
ORIGIN      
        1 atcacgaaca aagtcagatc ggctgatttc atggcccgta agcttcgggc aacgctctct
       61 ttttccagtt tgtcgctaat tttggctttt tttctgatgc cggcggtgtc aatcaacaga
      121 atcagcttat ttttgtatga aatcagagta tcgtttgttt cgcgggtcgt gtggggaatt
      181 tcgctgacaa tgactttttc cttgcctaaa atagcgttta ggattgatga tttaccaaca
      241 tttggtttgc caataatggc tattttaaaa agaatttccg gaatgacctg ggtaactttt
      301 ttaaatgaag gcagtcgtga cagctgactg acgacttcat ccaatagatc gccagtgccg
      361 gcgccggtga cggctgaagc cggccagaca tttttaacgc ccaaaaagtt tgtctgttcc
      421 actttttttc gcagtttagg gccgtcagct ttattggcca ccagcaaaaa tggttttttg
      481 gattgtttca tttttctgat taaatctttg tcctggatca gcaggccggc cctgatatca
      541 atcagcagta aaattaaatc agcctttttg atggccagct ccgcctgttc aataatattt
      601 ttttcaattt tttctttttt ggaaacatcc agcccgccgg tgtcaatcag attaaaataa
      661 tagccccgcc ataaacaaaa accaatatta gggtcccggg tggtgccggg cacttttgaa
      721 acaatagctt tttgctcttc agtcagccgg ttgaacaggg ttgacttgcc gacattggtc
      781 cggccgacga tggccacgtt ttgaagttgg ggcagttcca tatatgaata caggcacaag
      841 gcaataggca caaggcacaa ggcgaggcaa cagggattag gcattagtca aaaccctttg
      901 ccttatgctc atcaaataat cgtcccgcct aaggcgggac accaaccctt gtgcctcgtg
      961 cctgttgcct tgtgtcttaa ttagttcgcg tcccggccaa gaattattag aaagtccgcc
     1021 tgactgattt cctgattgcc ggaagttaac aagttggagg taatttcttt tttcaaataa
     1081 tcagtggttt tgggaaactg gttgttgttt aagctgtaga taactgtttt ttcataatca
     1141 tgagtctggg cgttgccaat attaatgaca tcgtagccgt cgttgacaag tttcagttca
     1201 gtcttggcgg ccagaccggt aatccaggtg ccgtttaaaa tttcaatggt agcttcttct
     1261 tttttggtga caactgtcac cggcagacta tcttgagtga taatttccag ttcaggttca
     1321 gcttgcttgt cagtttcagc cgcttcagcc gggtcaatct ctgaagtttt cggtgaaaat
     1381 tctccgagca aagttttggt tttattttcc atcgtataga caaaagtttg atcaaagatg
     1441 ttgctgacga ttgatttgat ctccgagaag tcatcagccc gcggttccag gacagaggcg
     1501 ccgtcataca tatttgccac cagcggcccg tccggaccgt tatccaggac ctggttgata
     1561 attcggttat tatcaaaatc cctggcgatc tccatcattc gactgatgtc gcttagttta
     1621 atgttagttt gaatgttgtc ttccagagta tttaatattt tcaaaatttt attgggactt
     1681 aataaaattt tataggacaa aactttatct ttaacggcca ggagcagttt ttgctggcgt
     1741 cgggaccggg caaaatctga attttcaggg ggcagggcgt gccgggagcg ggcgtatttt
     1801 agggccagct ttccgtccat ttcctgaagg ccttgttcaa tatagagatg ttcatatctg
     1861 gcttcttcgg tggcgtttgc tttgcctttg accggatatt ggtaatcatt caggctgttt
     1921 tccacaaaaa tttcaacgcc gccaacagta tctatcagct cttcaaagcc ggaaaaatca
     1981 attaagaaat gatactgtat cggcaggtct aaaagctggc tgatgaaatc agccgtgaat
     2041 ttggctccct ggccatcatt atcaattcct ccaaaaacat tcagggtgtt tattttccgc
     2101 cagccgtagc cgggcagggg aacgaccagg tcgcggggga ttgagatcag agccagttga
     2161 ccggaagagg gtttataact acccaaaata atcgtgtcag ttaaattagg tccctgatgg
     2221 cctttgccgc cgatgcccag taaaaggaag ttgattcggt cattttcttc gcctcgcaag
     2281 aggtgagatg atccggtcat ttgttttaac tggtcaaata aataattttg tgaaaaactt
     2341 tttatattgg caaagactga ttcatctgac ggaaagatga ttctggaaga aaaaaagaaa
     2401 aataaaattc ccagaacggc caccagataa aaactaaatt tagccagctt tagccaggga
     2461 tttttttctt taaaatcttt tgattccctg gtttgagggt ccagtaaatc tattttggga
     2521 tttttaatca taaactacaa aattttttat taattatact ggaattttgg ttaaaagcca
     2581 agcgtttgtt ttttcctaaa aacaatgtta taataggaaa aattaacaaa atttaatgaa
     2641 aaataaaaaa ccagcttatt tatttgaatc aaaaactgtt tttttgaaat catcggccga
     2701 gtttattctg gaactagtca aaattgtcat catttctctg gcgattatta ttccggtcag
     2761 atattttttg attcagccct tttatgtcaa gggcgcttca atggagccta atttttttga
     2821 tcatgaatat ctggtgattg atgaaatcag ctatcgtttt aaagaacccc agcggggcga
     2881 gatagtggtt tttaaatatc cccgcgaccc cagccagtat tttattaaaa gaattatcgg
     2941 cctgcccgga gacaaggtgg agatcaaaga cggccgggtt tatgttggta atttgaaact
     3001 ggatgaatcg gcttatttaa atcctgaaac taaaactttg ggcgacctgg tgattactct
     3061 ggaaggggat gaatattttt tacttgggga taaccgaaat ttcagcctgg attccagaag
     3121 ttttgggccg gttaagaaaa gctttattat cggacggacc tggtttcggg gctggccttt
     3181 cagcaagata atggtttttg aaaagcctct ctataatatt gaataattaa tgattatatt
     3241 aagctaaact ttagctgata aagaatttta gtattcttta ttttttttct atctatgccc
     3301 agaaaaaagg aagaaccaaa agaagagtta gtcgtcagcg tcaggaaatc tcccaaactg
     3361 gttcggggaa tgagagatgt tttgccgcag gatcaaaaat attgggccta tatttataat
     3421 aaaattgacg agctgaccgg aacttatcag tttgaaaaaa ttgaaacccc tattcttgaa
     3481 gaaacagccc tttatgtcag agggattggc aagaaaagcg atatcgttga aaaagaaatg
     3541 ttttcttttg ttgaccaggg cggctcaaat ttatctctcc ggccggaagc gaccgccgga
     3601 gtggtcagag cctatattaa ccacgggatg cacaatctgc cccagccggt ccggttgtat
     3661 ttaaccggca cttttaacag gcatgagcga ccccaggccg gccggctgcg ggaatttcac
     3721 cagtttgact gtgaagcttt gggagacgac agtccggttc tggacgctca ggtgattttg
     3781 attggtttta atttaattaa agaactgggt ttgcctgtcc aggtccaaat taacagtctt
     3841 ggctgtgaca cctgccggcc tgaatatatt gaaaaattaa gcagctatta caaaattcac
     3901 aaaaagaaaa tttgtgaaga ctgccagaaa agactgacta aaaatccttt aagattgctt
     3961 gattgcaaaa atgagcagtg ccaggaggtc aagctggaag cgccccagat tgttgactgg
     4021 ctgtgcgctg actgccggga gcattttatc aaagtcctgg aatatctgga tgagctggag
     4081 gtggcttatc atctcaatcc ccagattgtc cgggggctgg attattataa ccggacgact
     4141 tttgaatatt ttttggaaga tgaagaggat actaaaaccg ctctgggcgg gggaggccgc
     4201 tatgacggtc tggtagagtt gctgggtggt ccaagagaaa cgccggctgt gggcatggca
     4261 attggtttgg aaagactgat tgccaaaatc agggacaaaa atattccggt gcctgaaaaa
     4321 gacaccccgg atatttttct ggcccagtta ggtgagaagg ccaggaagaa atctttaatt
     4381 ttgtttgaaa agcttagaaa ggctggtttt aaagtggccg agagcttttc caaagacaat
     4441 ctgaaaaatc agctggaaac agctaacaaa ctgggagtga aatttacttt ggttctgggg
     4501 cagaaagaaa ttctggacgg cacgattttg atcagggata tggagggcgg tattcaggaa
     4561 gtggttgatt acaataaaat tgagcatgaa ataaataaaa gactgtctaa agtatagttt
     4621 gacggtattt tttataaatg ttatactttg ataataaaat taaattacaa ctagaaggga
     4681 gtagtgataa aatt
//
LOCUS       DASWHZ010000048         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_114708, whole genome shotgun sequence.
ACCESSION   DASWHZ010000048 DASWHZ010000000
VERSION     DASWHZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114708"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VGA49_01105"
     CDS             <1..447
                     /locus_tag="VGA49_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664399.1"
                     /translation="LTTRHNHKNKEERDALIKALVCAIIRWGESENKRFAIATAPQWL
                     ITRVQKRLGFNPIHVIGRNPVKKRIPEIFLPYFASTFKFMEAKGKKGRRNARPPHDLV
                     RAIWLDLREIGPTVNAIREQMFSEAVTDNGPVIILENHRQPQKAAG"
     gene            633..>2555
                     /locus_tag="VGA49_01110"
     CDS             633..>2555
                     /locus_tag="VGA49_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9664400.1"
                     /translation="MKNRLLGLFIFSLVGWNSSILLLQITQNFFWAQTSFFSASFISL
                     TLLLFAFVFPYSLKIKRIYKILLILPAGFFALIALFRPYLLYSEITFSNTSIIYKNQS
                     LHPAFLGFFLGYFMAAIIILLYKYKQSKGIHLVQLKYFFWGLFVALFLIMLVSLILPS
                     FGVYQYNGLGPPLTSLITIAIAYAIIRHQLMDIGLAIKKSTVYISSILATTLIAAGGA
                     YILYNILSLNPVIAGPAILIFGLIIFSAIKPWFEVLGNKYLFVSTYNYQQSIKELTRD
                     LIKTIDLDQISDSIIGTIRETMRLKKAKILLLETDDSQRHYKLIKNIGFDPNEKLIDQ
                     VINEHLKVYFNNFKKPLVYELIDDKIVDPKIQKNIEIINNLINLKNKLQNLPVAVYIP
                     MIHHNELIGLILLGEKISKDSFSKEDLSLLETLANQAAISIANARLYSQVQNLNKNLQ
                     QRVDEQTRHLHELLEMKSEFLTIASHQLRTPTSIIKGMLSTLLDKTINLENDKKENFL
                     QKAFQGSLRLEKIVSDLLKTSQLAAGKMETEAKPIDIGPIIEKLVSSRLPLAEEKKVN
                     LKYNKPGEIPKVVADPIKIFEAIANLIDNALHYTDQGQVEIKFLDPDTDKNYLKLSIR
                     DTGIGIPPEEIEIICEK"
BASE COUNT          786 a          580 c          487 g          702 t
ORIGIN      
        1 ctgacaaccc ggcataatca caaaaacaaa gaagagcgtg acgccctcat caaagcgctg
       61 gtttgcgcca tcatccgctg gggtgagtcc gagaataaac ggttcgccat cgcgaccgct
      121 ccgcaatggc tgattaccag ggtccagaag aggttgggct tcaatccgat acatgtcatc
      181 ggacgaaacc cggtcaaaaa aaggatcccg gaaatcttcc ttccgtactt cgcttccact
      241 ttcaaattta tggaagcgaa ggggaaaaaa ggcagaagaa acgccaggcc gccccacgat
      301 ttagtcaggg cgatttggct tgatctccgg gaaatcggac cgaccgtcaa cgccattaga
      361 gaacagatgt tctctgaggc ggtaacagac aatggcccgg tcattatcct ggagaaccac
      421 cgccaaccac aaaaggcggc gggctgaaac aggaggaccc agtcagttgt tttacaactg
      481 actttttttt acacctgaac cccttgcaaa aaaactggtt ttgtattata attcggttga
      541 aatatatgat tatatatata acaattttag ctttgattat cctaatcaac atttccttag
      601 gaattattgt cttatataaa aatccgaaca atatgaaaaa caggctactt ggcctgttta
      661 tttttagttt ggtcggctgg aatagttcaa ttttgctatt gcaaataact caaaattttt
      721 tctgggcgca aacctccttt tttagcgctt cattcatcag cctaacacta cttttattcg
      781 cttttgtttt tccttactct ttaaaaatca aaaggattta taaaatacta ctgatcctgc
      841 ccgctggttt cttcgcccta attgctctgt tcaggcctta tttgctttat agtgaaatta
      901 ctttcagcaa taccagtatt atttataaaa accaatcgct ccatccagcc tttctggggt
      961 tttttctggg ctacttcatg gcggcaatca ttattctgct ttataaatac aaacaatcaa
     1021 aggggatcca tctggtccag ttaaaatatt tcttctgggg tttatttgtc gctctatttt
     1081 tgataatgct tgtttcttta atcctgccaa gtttcggcgt ttatcaatac aacggactcg
     1141 gcccgccatt gaccagcctg attacaatcg ccattgccta cgccatcatc cgccaccagc
     1201 tgatggacat cggtctggcc attaaaaaaa gcacggttta tatcagctct atcctggcaa
     1261 caacgctgat cgcggccggc ggagcttata ttctttacaa tatcttgtct ttaaacccgg
     1321 taatcgccgg gccggcgatt ctgattttcg gcctgattat ttttagcgcg atcaaaccgt
     1381 ggtttgaagt tttaggcaat aaatatttat tcgtcagcac ttacaattat cagcagtcca
     1441 tcaaagaact gacccgcgat ttgattaaaa caattgatct cgatcagatc agcgactcaa
     1501 ttatcgggac aattcgggaa acaatgcgtc tcaaaaaagc caagattctg ctgctggaaa
     1561 ctgatgatag tcagcggcat tataaattga ttaaaaacat tggctttgat cccaatgaaa
     1621 aactgattga ccaggtgatt aacgaacact taaaagtcta ttttaacaat tttaaaaaac
     1681 cactggtcta cgagcttatt gacgataaaa ttgttgatcc caaaattcaa aaaaacattg
     1741 aaatcatcaa taatttaatc aatctaaaaa ataaactgca aaatctgccg gtggccgtct
     1801 atatcccaat gatccaccac aatgaactga tcgggctgat tcttttggga gaaaaaattt
     1861 ccaaagactc cttcagcaaa gaagatttgt ctttgctgga aactctggcc aaccaggcgg
     1921 ccatttcaat cgccaacgcc cggctctaca gtcaggtcca gaatcttaac aaaaatctcc
     1981 agcagcgggt tgatgagcaa acccggcacc tccatgaact gctggaaatg aaatcagaat
     2041 ttttaaccat tgcttctcat cagctaagaa cacccacctc cattattaaa ggaatgttgt
     2101 caactctgct ggataaaacc atcaatttgg aaaatgataa aaaagaaaat tttctgcaaa
     2161 aagcttttca gggctcactc cgactggaaa aaatcgtcag tgacctttta aaaaccagtc
     2221 agctggcggc cggcaaaatg gaaactgaag ctaagccgat tgacattgga ccgattatag
     2281 aaaaacttgt cagcagccgt ctgccgctgg ccgaagaaaa aaaggtaaat ttaaaatata
     2341 acaaaccagg agaaattccc aaagtcgtgg ccgatccgat taaaatcttt gaggccatcg
     2401 ccaacctgat tgacaacgcc cttcattata ccgatcaggg acaggtggag attaaatttt
     2461 tagatccgga tactgacaaa aattacttaa agctgtctat ccgggatacc ggcattggca
     2521 tcccgcccga agaaatagaa atcatttgcg aaaaa
//
LOCUS       DASWHZ010000049         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2359488, whole genome shotgun sequence.
ACCESSION   DASWHZ010000049 DASWHZ010000000
VERSION     DASWHZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359488"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1341
                     /locus_tag="VGA49_01115"
     CDS             19..1341
                     /locus_tag="VGA49_01115"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX9664401.1"
                     /translation="MTLITMSSKEIDRFQVIKKLIGKHIDEIKAAKLLRLSVRQIRRL
                     KSKVRQSGAKGLIHGNRGKESHNRINENKKKKIIVLLRKHYHDFGPTFASEKLSENHG
                     VNHDSKTIRQIMIDEGLWKPRTKKKQQKHLQWRERKSCYGEMIQFDGSYEHWFEDRND
                     TGEICLLAAIDDATGRPVSLIFAKHEGVFPVFGFWRRYTVKNGKPKSIYLDKFSTYSM
                     NHKLAKENSDTLTQFERAAGELKIELIKANSPEAKGRVERLFGTLQDRLIKELRLANI
                     SDIAAANIFLEKTFIPKFNAKFSVEPRNKTDLHQKLTQKENKQLQGIFSRQRERTVLN
                     DFTFSFNKQWYQLIKEQPATICKKDKVIVEEHLDHSIHVRLRSKYLNYHILPNRPLKT
                     NKTKPWVIAKTQEEVNQSKAHKPPADHPWRQTIKAPVVRQLSAPNRTF"
     gene            1537..2184
                     /locus_tag="VGA49_01120"
     CDS             1537..2184
                     /locus_tag="VGA49_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664402.1"
                     /translation="MNNQNRISRGGFRVVQRIFGIAMVVGLCIFVPYLMISLLFLLFI
                     EVPKPVPQVPVYVQEAGIDEDKGLATYTVSGIAVHRSAEGQTPISWTTSDGKPLFPIR
                     QDIDAFPAHIYISYINRIDGITYILVEAPGYKGRGMAYHGFRFFAYDGKTLNQCWELE
                     FWPLAHPPIDGIFYRPCQVVVVNHERVDVYGANFKIEKDGTNIWFWKLIKQQYFR"
     gene            2385..3023
                     /locus_tag="VGA49_01125"
     CDS             2385..3023
                     /locus_tag="VGA49_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEX9664403.1"
                     /translation="MHQIQEKLLDLVKEEDVSQLTLRQIAKLVGEKHPQKIKHHLNQL
                     EKKGMIDIDWHSGSVKRNSRRADKNSSFQSVPIIGLADCGQAITFAEETFEGYLKVSR
                     KILDKIRNIFAVQAKGNSMNKAKINGQTIEDGDYVVIDSSDKDPFNGDYVLAVVDNFA
                     TIKRFYRDRQTNRIVLLAESTEEYLPIYLHEDDSFMINGIVKFVLKKPKINF"
     gene            3207..3416
                     /locus_tag="VGA49_01130"
     CDS             3207..3416
                     /locus_tag="VGA49_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664404.1"
                     /translation="MSVETKTFKIIKSGLFQSREARLDKFFALPRFAKQGGGGNPEDL
                     KVVWRQMDIIKKIKRKMVRSRSLFL"
     gene            3413..3604
                     /locus_tag="VGA49_01135"
     CDS             3413..3604
                     /locus_tag="VGA49_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HEX9664405.1"
                     /translation="MTGQNRDDIKPGMEVWVVLKKDQATGQLTKGIVKDILTSSAHHP
                     RGIKVRLEDGQVGRVREVV"
     gene            3619..>3799
                     /locus_tag="VGA49_01140"
     CDS             3619..>3799
                     /locus_tag="VGA49_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664406.1"
                     /translation="MDKKNKAEILLLVTLAVFLIQSLGICDTEQCKAASKTADLPMEW
                     GFTYDFPHEIVVATSG"
BASE COUNT         1204 a          756 c          838 g         1001 t
ORIGIN      
        1 tcattaaccg tttcatctat gacgttaatc actatgtcct caaaagaaat agacagattt
       61 caagttatca agaaattaat cggcaaacac atcgacgaaa taaaggcggc gaagttgctc
      121 cgtttgagcg ttcgccaaat cagacgtctg aaatccaaag tgagacagtc cggagccaaa
      181 ggtttaatcc acggcaaccg cggcaaggaa agccataacc ggataaatga aaataaaaag
      241 aaaaaaatca ttgtcctttt acggaaacat taccatgatt tcggtccaac ctttgccagc
      301 gagaaattat ctgaaaacca cggcgttaat catgactcca aaaccatccg tcaaataatg
      361 attgacgagg gactgtggaa accccggaca aaaaagaaac aacaaaaaca cctgcagtgg
      421 cgggaaagaa agtcttgtta cggcgagatg atccaatttg acggttcata cgagcattgg
      481 tttgaagaca gaaatgacac aggagaaatc tgtctgctgg ccgccatcga cgatgccacc
      541 ggccgtccgg tttctctcat atttgccaaa cacgaaggtg tatttcctgt ttttggcttc
      601 tggaggaggt acaccgttaa aaacggcaaa ccaaagtcaa tttacttaga taaattcagt
      661 acatacagca tgaatcacaa attggccaaa gaaaacagcg acaccctgac tcaatttgaa
      721 agggcggccg gcgaattaaa gattgaactc attaaagcca acagccccga ggctaaagga
      781 cgggtggaaa gattgtttgg taccttgcaa gacagattga ttaaagaatt aagattggcg
      841 aatatttccg atattgccgc ggccaacatc tttttggaaa aaacattcat ccctaaattc
      901 aatgccaaat tttcagttga accgagaaac aaaaccgact tgcatcaaaa attaactcaa
      961 aaggaaaaca agcaattaca gggcatattt tcaagacaga gagaacggac agtgctaaat
     1021 gactttactt tttcatttaa taaacaatgg tatcaactaa taaaggaaca gccggcgact
     1081 atttgcaaaa aagacaaggt aattgtcgaa gaacatctgg accattcaat tcatgttcgt
     1141 ttgcgaagca aatacttgaa ttatcatatt ttgccaaata gaccgcttaa aaccaacaaa
     1201 acaaaaccgt gggtaatcgc caaaacgcaa gaggaggtta atcaaagcaa agctcataaa
     1261 ccgccggccg accatccttg gagacagaca ataaaggccc cggttgtccg gcagctgagt
     1321 gcgccaaata ggacatttta acttccctga aagtaggaca ttttaacttc ccgttgacaa
     1381 actctacatt ttcttgacta aattcaaaaa atgtgataat atcgattatt caaatttttt
     1441 gctctttgaa agtttgacga tagctctttg attatcttaa tctaaagtat ttaaggagct
     1501 atcgtcagaa acatccgaga acaaggaggg ttggtgatga acaatcaaaa ccggatttcc
     1561 cggggcgggt tcagagttgt ccagagaatt tttggtatcg caatggtggt tgggctatgc
     1621 atttttgtgc cttacctgat gatcagtctg cttttcctgc tttttatcga agtgcccaag
     1681 ccagtcccgc aggtgccagt ctatgttcag gaggcaggca ttgacgaaga caaaggtctg
     1741 gcgacttaca ccgtctccgg catcgcagtc catcggtcag ccgaaggaca gacgccgatt
     1801 tcctggacta cttctgacgg aaaacctttg tttccgatca ggcaggatat tgacgccttt
     1861 ccggcccata tctacattag ttatatcaac cggattgacg gaatcacata catcctggtt
     1921 gaagcgcctg gttacaaagg ccggggaatg gcgtatcatg gttttaggtt ttttgcctat
     1981 gacggaaaaa cgttgaacca gtgctgggag ctggaattct ggcctttggc tcatccgcca
     2041 attgatggaa ttttttatcg gccctgtcag gtggtagtcg ttaaccacga gagagttgat
     2101 gtctatgggg caaatttcaa aattgaaaag gacggaacca atatctggtt ctggaaactg
     2161 ataaaacaac agtattttcg ttagtttaac cactctcatg ctgtcaagca agggagtttt
     2221 tttttatatt ttaagatttt gtcaacaata aatttgacaa acttgacaag attttttagt
     2281 aaggtaaaat tatattgtag ttgtggataa attctcttca tctcccctca tcaactcccg
     2341 atcccgccat aggcgggatc gggaccttat caaccctcct tgctatgcac caaatccaag
     2401 aaaaacttct tgatctcgtt aaagaagagg atgttagcca gttaactctc cgccagattg
     2461 ccaaattagt cggcgaaaag catcctcaaa aaatcaagca ccaccttaat caattagaga
     2521 aaaaggggat gattgatatt gactggcatt ccggcagtgt caaaaggaac agccggcggg
     2581 ctgataaaaa ttccagtttt cagtcggtgc cgattattgg tttggctgac tgcggccagg
     2641 cgattacttt tgccgaagag acctttgaag gctatctcaa agtttcccga aagattttgg
     2701 ataaaatccg gaatattttc gctgtccagg ccaaaggcaa ttcaatgaat aaagctaaaa
     2761 ttaatggcca gacgattgaa gacggcgatt acgtggtgat tgattcatcc gataaagatc
     2821 cgtttaatgg tgattatgtt ctggcggtgg ttgataattt cgcgaccatc aaaagatttt
     2881 accgtgaccg gcagactaac cggatagttc tgctggccga gtcaaccgaa gaatatctgc
     2941 cgatttattt gcatgaagat gatagtttta tgataaatgg gatagttaag tttgttttga
     3001 aaaaaccaaa aataaatttt taaaaaatac ctctctttta ctttttgatg gcaaaaagta
     3061 acaaaaacca ctggggtagc caattcgcct gtgtgctatt taaataatca gcttttgcct
     3121 cgggctcaag gctaccccag accccttagg tgggagagga aatttatcag agatatctct
     3181 aacttttaac attaaatgaa agttttatgt cagtagaaac aaaaacattc aaaatcatca
     3241 aatcaggcct gtttcaaagc cgagaagctc ggttggataa attttttgcc ttgccccgct
     3301 ttgcgaagca gggcgggggt ggtaaccccg aagatttaaa agttgtctgg cggcagatgg
     3361 atataatcaa aaaaattaag aggaagatgg tcagaagccg gagtttattt ttatgactgg
     3421 acaaaacaga gacgacatca aacccgggat ggaggtttgg gtggttttga aaaaagatca
     3481 ggccaccggc caattgacca aaggaattgt caaagatatt ttaaccagct ccgcccatca
     3541 tccccggggg ataaaagtca ggctggagga tggtcaggtg gggcgggtgc gggaggtggt
     3601 gtaatttaat aaaaatttat ggacaaaaaa aataaagcag aaatattgct tttagtaact
     3661 cttgcagtgt tccttattca gagtctaggt atttgtgata ccgaacagtg taaggcggct
     3721 tctaaaacgg ctgacttgcc aatggagtgg ggctttactt atgattttcc gcatgaaata
     3781 gttgttgcaa catcaggtg
//
LOCUS       DASWHZ010000050         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_507576, whole genome shotgun sequence.
ACCESSION   DASWHZ010000050 DASWHZ010000000
VERSION     DASWHZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507576"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..522)
                     /locus_tag="VGA49_01145"
     CDS             complement(97..522)
                     /locus_tag="VGA49_01145"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX9664407.1"
                     /translation="MSYRFLEHEADMGIRAEGRSWEEVFTEGAKAVFDLMIELKEFKP
                     KKEVKIKCQAPDIGSLFVEWLNELIYQKDVTGIYFIKFEIDSIKQKNQNYQLTGKAWG
                     EKLDPDKHTLETEVKAATYGGLECKEEKGKYYCQCVVDV"
     gene            complement(613..1299)
                     /locus_tag="VGA49_01150"
     CDS             complement(613..1299)
                     /locus_tag="VGA49_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664408.1"
                     /translation="MNVLETWGEVLATSFSNLWMAFVDFLPKFLGAVIILIIGWIIAA
                     ILGNIAAKIIDFLKIDEALTKLNLKKGLEKAGIEFTIAGLIGWLVKWFLIIVFLIAAA
                     DILEWVEVTDFLKNVVLYIPNVIIAVIILLAGILVADFVNKVIKESVKAAKLKSASFL
                     GGLAKWAILVFSFMAALVQLSIANALIQTLFTGFVAMLAIGGGLAFGLGGKDLATKAL
                     ERMRKDISEK"
     gene            complement(1386..1670)
                     /gene="yccX"
                     /locus_tag="VGA49_01155"
     CDS             complement(1386..1670)
                     /gene="yccX"
                     /locus_tag="VGA49_01155"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9664409.1"
                     /translation="MNLARLKIVVYGNVQGVFFRQNTQEKALKLGLTGWVRNNSDGTV
                     EIIAEGPKHKLEMLLNWCQAGSKEAWVESVSFEWISYKKEFQDFKIKCRR"
     gene            complement(1705..2355)
                     /locus_tag="VGA49_01160"
     CDS             complement(1705..2355)
                     /locus_tag="VGA49_01160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9664410.1"
                     /translation="MNKIFLAFSFFALLLLAGCLHNKNIPVNENLNNNINQPASDVKQ
                     ELEDALIRFPDENAMAVIKTNLGEIKVKFYSLDAPNTVNNFIKLSNDGFYNGTKFHRV
                     IKDFMIQGGDPNSKDDDWSDDGFGGPGYKFPDEINDNKLVSGVLAMANSGPNTNGSQF
                     FIVTKESTPWLDGLHTAFGEVIEGLEVVDKIEAVETNARDHPITDVIVQEIVIINQ"
     gene            complement(2508..>2829)
                     /locus_tag="VGA49_01165"
     CDS             complement(2508..>2829)
                     /locus_tag="VGA49_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HEX9664411.1"
                     /translation="AMAEIVSRRYYRQVTENGEKPDLIVIDGGQGQVNAAYRALSSLG
                     LNIPVIGLAKRLEEIYFVNESQPLLLSHKLESLKLLQSIRNEAHRFAISYHRLLREKR
                     IRGK"
BASE COUNT          820 a          670 c          460 g          879 t
ORIGIN      
        1 ctctgttcct cagtttcttt gttcttctgt ttctctgttc ctcagttcct ctgttcctca
       61 gtttctttgt tcttctgttt ctctgttcct cagttcctac acatccacca cgcactgaca
      121 ataatatttc cccttttctt ccttgcattc cagcccgccg taagtggccg ctttgacttc
      181 cgtttctaag gtgtgtttat caggatccag tttttcgccc caggctttgc cggttaactg
      241 ataattttga tttttttgtt taattgaatc aatttcaaat tttataaaat aaatcccggt
      301 aacatccttt tgataaatca gctcattgag ccattcaaca aacaaactcc caatatcggg
      361 agcctggcat ttaattttaa cttctttttt gggtttaaat tctttcagct caatcatcag
      421 gtcaaaaacc gctttagcgc cttcggtaaa aacctcctcc cagctccggc cctccgccct
      481 gatacccatg tccgcttcat gctccaaaaa acgatacgac attgttgtaa aataatttta
      541 acattcgtaa gctccgaaaa cagttcggag caaacgaata ttaatttatc tctagttaac
      601 tttagacaaa tgttattttt ccgaaatatc ttttctcatt ctctccaaag ctttggtggc
      661 caaatccttg ccgccaagac caaaagccag accgccgcca atagccagca tggccacaaa
      721 accagtaaat aatgtctgga tcagagcgtt agcaatgctt aactggacta aagcagccat
      781 aaagctgaaa actaaaattg cccatttagc taacccgccc aagaaagagg ccgattttag
      841 cttagcggct ttgactgatt ctttgataac cttattgaca aaatcagcca ccaaaatacc
      901 agccagcagg ataatcacgg caataatgac atttggaatg taaaggacaa cattcttcaa
      961 aaagtctgtc acctcaaccc attccaaaat atcagccgcg gcaatcaaaa agacgataat
     1021 cagaaaccac ttgaccagcc agccaatcag gccggcaata gtaaactcaa tcccggcttt
     1081 ttccagaccc tttttaagat tgagtttggt caaagcttca tcaattttta aaaaatcgat
     1141 gattttagcg gcaatattgc ccaggatagc ggcgataatc cagccaataa tcaggatgat
     1201 gaccgcgccc aaaaatttag gcaaaaagtc aacaaaagcc atccaaagat tactgaatga
     1261 agtcgccagc acttcacccc acgtctctag tacgttcatt tggtttttca cctcccttcc
     1321 cagtcccgcc taaggcggaa ctttttaatt tttattttaa taccaccgcc tttggcgggg
     1381 tgccgtcaac ggcggcactt tattttaaaa tcctggaact cttttttata actaatccat
     1441 tcaaagctga ctgactcaac ccaggcttct tttgacccgg cctgacacca gtttaaaagc
     1501 atctccagct tatgtttagg accttcagct ataatttcca ctgtcccgtc cgaattattt
     1561 ctaacccagc ctgttaaacc caattttaaa gctttttctt gtgtattttg tctaaaaaag
     1621 acaccctgga catttccgta aacaactatt ttcaatctgg ccagattcat atatccacag
     1681 gctgttattt aattatacta tattttactg attaattata acgatttcct gaactataac
     1741 atcagttatg ggatgatctc tggcgtttgt ttcaacggct tcaatcttat ccacaacctc
     1801 cagtccttca atgacttcgc caaaagctgt atgcaaacca tccagccagg gcgtggactc
     1861 ttttgtcaca ataaaaaatt gactgccatt ggtgtttggt ccggaattgg ccattgccaa
     1921 aacgccgctg accagcttat tgtcattgat ttcatcagga aatttataac caggcccgcc
     1981 aaaaccgtca tcagaccagt catcgtcttt gctgttcggg tcgccgccct gaatcatgaa
     2041 atccttaatt acccggtgaa atttagtgcc attataaaag ccgtcattag acagcttaat
     2101 aaaattattg acggtattgg gagcgtccag gctgtagaat ttgactttaa tctcgcccag
     2161 attagtttta atgacagcca tggcgttttc atcgggaaat ctgatcaagg catcttccag
     2221 ttcctgcttt acgtctgaag ccggctgatt tatgttatta ttaagatttt cgttaactgg
     2281 tatattttta ttatgcagac agccagctaa aagcaataaa gcaaagaaag aaaaagctaa
     2341 gaagatttta ttcatataat ataataagca ccaaattcaa aactcgaaat cctaaataaa
     2401 tcataaattc aaatgttcaa aattcaaaac ttgtaattgt ttattttgaa tttatttgga
     2461 gcttatgatt ttcaatttag aattttcgtt aattatagca cgattctcta ttttcctcta
     2521 attctctttt ctcttaataa acgatgataa ctgatcgcga accggtgagc ctcatttctg
     2581 atgctttgaa gcaattttaa gctttccagt ttatgactta aaagcaaagg ttgtgactca
     2641 ttaacaaaat aaatttcttc caaacgtttg gctaaaccaa taaccggaat atttaaaccc
     2701 aaggaactta aagctctgta ggccgcgtta acctgaccct gaccgccgtc aatcacgatt
     2761 aaatcaggtt tctcgccgtt ttctgtgacc tggcgataat accggcggga gacaatctcg
     2821 gccatggcc
//
LOCUS       DASWHZ010000051         7369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2722002, whole genome shotgun sequence.
ACCESSION   DASWHZ010000051 DASWHZ010000000
VERSION     DASWHZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2722002"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VGA49_01170"
     CDS             <1..183
                     /locus_tag="VGA49_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664412.1"
                     /translation="SIENISKFGQRVTTPSFATTVDKLSGDLKDKSEIASQSKKSADS
                     IDEDLEIPAFIRKKMM"
     gene            282..749
                     /gene="nrdR"
                     /locus_tag="VGA49_01175"
     CDS             282..749
                     /gene="nrdR"
                     /locus_tag="VGA49_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX9664413.1"
                     /translation="MKCPVCQNTETKVTDSRVALDSASIRRRRECVKCNFRFSTIEQM
                     TILDLAVIKRDGRREPYSREKLERGLVRALEKRPYTKDKFQGLVNQIETDIQKLKKSE
                     ITSHDLGEVVMKRLKKFDKVAYIRFASVYRSFEDVKTFQRELSKLANKKRKKK"
     gene            760..1011
                     /locus_tag="VGA49_01180"
     CDS             760..1011
                     /locus_tag="VGA49_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664414.1"
                     /translation="MVEDFKDLIEKSLKLQRETLKLVKKIRRSLLFSRILGIIFIIMF
                     IVLPVVAAFTFLPPFLKNFFAAYDQILRVAEELKGLPGL"
     gene            1044..2264
                     /locus_tag="VGA49_01185"
     CDS             1044..2264
                     /locus_tag="VGA49_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9664415.1"
                     /translation="MDIYPTNYSPENSLKNKGSFSSPIAMAVFVAAITGAIAGASVAG
                     YIIENGQLVVEEESATTAAVEKVSPSVVSIIISKDISGDFNQTGPNIFPFDNFFDFGF
                     PFDFRFRSTEENNQAPRKQEIGGGTGFIISEDGLILTNKHVVLDLEADYTVLTNDGRE
                     FEATVLARDQINDIAIVKIEASGLSVVELGNSDNLKLGQTVIAIGNALGEFRNTVTKG
                     IISGIDRRVLAGNGQQSEVIEEAIQTDAAINPGNSGGPLINLSGQVVGINTAVSQAGQ
                     LIGFAIPVNLAKNAIDSVREFGRIVRPYIGVRYVMVNEGIAEENNLNVDHGALIVQGD
                     EDQLAIVVNSPAEKAGLQSGDVILEVNGRRIDLENSLAHEIVKYRPGDQAELKISRDG
                     QEQIVKIILGEFEE"
     gene            complement(2244..4292)
                     /locus_tag="VGA49_01190"
     CDS             complement(2244..4292)
                     /locus_tag="VGA49_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664416.1"
                     /translation="MIDYKKFILPTALIIIIFIINLVAVFPLLQGEHSQFTESIAVSH
                     AISSGYFINLQNVINPFWYTGFTKQIVYPVLSLILISLPTLFIKGLSIFASYRIITGL
                     FYILIPCSLFILLKYLTKNNLIPFFLTLIYSVGPAVSASFLPIEGALAEFNIFLPWRL
                     LVYTLFGEGPHIISLFFIPLGLIVYIKLLKKGGLKSFILFILIASAVLLTDLFGALAL
                     ALICLIILISELFLKNSQNKFKTSFWALMAILGLIAFHYNPYFFELIFKSGYAHRENS
                     LSPGWPLIWILLILALFIFFYFFENKYKKNQLLQHSLVFTGAVVVLGLITVLAFYFDY
                     SLLPQAKRYVPEFNLAAITLISLIGLYLHDSFKLSKKFIIKHNLPAILALILAASLYN
                     FWPSAQTVIKPGDITQSTEYKIAQYLTETTSQLSGQKIYLTGPPSFWLNAFSDLAQIK
                     GAQDFVQPSRWWADIVYQIEKGEDPRIAISWLKLLNVQKIVVNNQLVSTPFMGYQNFT
                     RPNKFVDQLRLEEVIDDSNIYSVNLINSDLVQLVDFKNIKQYSAISNVLDQKNLAGLV
                     KTIEKGPANYQLNYDINQPDLIQIKVTAGQSDQALLFKSFYNPNWRAFINNQKYPIKK
                     FGPDLMLIEPNLAGDYTIEIKAAKSLSQKIGATITLLTLIILIIISVSKKKPLFLKLS
                     "
     gene            complement(4298..5716)
                     /locus_tag="VGA49_01195"
     CDS             complement(4298..5716)
                     /locus_tag="VGA49_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664417.1"
                     /translation="MAKIWRWTIIIFLLITVFSVFWRVPVSFDTGWHLASGRYYLENK
                     TIPQTDIFSQTMPDFNWVNHEWLTDVVLYSLYSRFGLVSLTIFYAVMLLLTFYLITGL
                     VKTNWIYRLLTTALASLAVFSVLGPKPQMITLFGLALILVIYNRYQTNPKIIFLIPFI
                     LLLWANLHGGFIIGLGLLFILIVFETVQKRKIKLLTALLAMSVLITLINPYGFQIYGE
                     IFKTAADTLGNSLAKATISEWQPVSFNGLRAYYFWPYLILTLILTVVFRKKLSSLYII
                     LSVIFLPLALSSWRHTPFFVLFSVPIYLESFSLLSEKSKIFRQLTNSWPLIIILSPFV
                     YLALLNIFAISFSLNWPVIAAQKTLPQGAVRFIQANSDQFQGKMYNKYDYGSYLTFFL
                     PEHKVFIDGRMLHWRQNNRSIYQDYFDIIHNFNFDLLDRYNIDWALLARNSVAAQKLF
                     AEEKWQLKYLDSDAIILARPQK"
     gene            5790..6954
                     /gene="prfB"
                     /locus_tag="VGA49_01200"
     CDS             join(5790..5855,5857..6954)
                     /gene="prfB"
                     /locus_tag="VGA49_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203067.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9664418.1"
                     /translation="MTELIKEIKDLRKKISGTWRLLDLDKKQVQIKNLTREVEQPGFW
                     DNQEQAKKVSKELADSQAEVSQWQTMKDQAKEILELAELAEKEKTDGDLEKDLRKKLA
                     RLSQEFEKLEFFVLFSDKYDSHNAIVSIHAGAGGTEAQDWAQMLERMILRYCENKKFK
                     TVIADQAVGSEAGIKSVTFRVEGKYSFGYLKSEAGVHRLVRMSPFDAEKMRHTSFALI
                     EVLPELAETDETKIDPNDLRIDTFISSGPGGQGMQRSYSAVRITHLPTKTVVTCQNER
                     SQVQNKATAMKILQSRLQQLQQFEQEKEKKKIRGEYLSAQWGNQIRSYVLHPYKMVKD
                     HRTDFEVKEPDVVLDGGVDGFVEAYLRWVKRSGKFKVSKSKVQSSKSKVSAKG"
     gene            7096..>7369
                     /locus_tag="VGA49_01205"
     CDS             7096..>7369
                     /locus_tag="VGA49_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9664419.1"
                     /translation="MKILVTGGAGFIGSHVVDALIAKKHQVVVIDDLSTGRKEFINKK
                     AKFYKLDIRSKELLKVFKKEKFAIVNHHAAQKDVRLSVKDPVFDAEV"
BASE COUNT         2381 a         1310 c         1689 g         1989 t
ORIGIN      
        1 tccattgaaa atatttcaaa atttggccag cgggtgacta cgcctagctt tgccacgaca
       61 gttgacaagc tttccggcga cctgaaagat aaatcagaaa tcgccagtca gtcaaaaaag
      121 tcggctgatt caattgatga ggatctggaa atcccggcct ttatccggaa gaagatgatg
      181 taggattagg gtatagggta tagggtatag gggatagatt aatagggtat agaaaacttc
      241 tccttgaata gggggagttt tgatgttaag atatatttaa tatgaaatgt ccagtctgcc
      301 aaaatacaga aaccaaagtg actgattcac gggtcgcgtt ggacagcgcc agcatcaggc
      361 ggcgccggga atgcgttaaa tgcaatttta gattttcaac aattgagcag atgactatcc
      421 ttgatctggc ggttattaag agagacggcc gccgggagcc gtacagccgg gaaaagctgg
      481 agcggggcct ggtcagagct ctggaaaaaa gaccttacac caaagataaa ttccagggac
      541 tggtcaatca gattgaaacc gatattcaaa agctgaaaaa atctgaaatt accagtcatg
      601 atctgggcga agtggtcatg aaaaggctta aaaaatttga taaggtggct tatatccggt
      661 ttgcttctgt ctaccggtcc tttgaagacg tcaaaacatt tcaaagagaa ctaagcaaat
      721 tagcgaataa aaaaagaaag aagaaataat cgtatattta tggttgagga ttttaaggat
      781 ttaattgaaa aaagcctgaa gcttcagcgg gaaaccttga agctggttaa aaaaataaga
      841 cggagtctgc tgttcagccg gattttggga attattttta taattatgtt tattgttttg
      901 ccggtcgtgg ccgcttttac ttttttgcca ccgtttttaa aaaatttttt cgcggcttac
      961 gatcagattc tgcgagtggc cgaagagctt aaaggcctgc cgggtctcta aagcctatac
     1021 cctataccct ataccctaga aatatggata tctatccgac taattattcg ccggaaaaca
     1081 gtctaaaaaa taaaggttcg ttttcaagcc cgattgccat ggccgttttt gtcgcggcga
     1141 ttaccggagc aatcgccggg gcgtctgtcg ccgggtatat tattgaaaac ggtcagctgg
     1201 ttgttgagga agaatcagcc accaccgccg ccgttgaaaa agtttctccg tccgtcgtca
     1261 gtattatcat cagtaaagat atttcaggag actttaatca aactggtcca aacatttttc
     1321 cttttgataa tttttttgat tttggttttc cttttgactt tcgcttccgg tcaacggaag
     1381 aaaataatca ggcgccccgc aaacaggaaa ttggcggcgg gacaggcttt atcatttcag
     1441 aagatggttt gatcctgacc aacaagcacg tcgtgcttga tttggaagct gattacacgg
     1501 ttttaaccaa tgacggccgg gagtttgagg caacagtcct ggctcgtgat cagattaatg
     1561 atattgccat tgtaaaaatt gaagccagcg gcttgtcggt ggttgaactg ggcaattctg
     1621 ataatttaaa actcggtcag accgtcatcg ccattggcaa cgctttgggt gaatttagaa
     1681 acacagtcac taaaggaatt atcagcggca ttgaccggcg cgttctggcc ggcaatggcc
     1741 agcagtcaga agttattgag gaagcgattc agactgacgc cgccattaat ccgggaaatt
     1801 ccggtgggcc gttaataaat ctttccggac aggtagtggg cattaacacg gcggtcagtc
     1861 aggccggtca gctgatcggc tttgccattc cggttaattt ggctaaaaac gccattgaca
     1921 gtgttcggga attcggccgg atagtccggc cttacatcgg tgttcgttac gtgatggtta
     1981 atgaaggaat cgccgaagaa aataatttaa atgttgatca tggagctttg attgttcagg
     2041 gtgacgaaga ccagctggcg attgttgtca acagtccggc tgaaaaagct ggtttacagt
     2101 caggtgatgt tattctggaa gttaacggac ggcggattga cctggaaaat tcattggctc
     2161 atgagattgt caaataccgg ccgggtgatc aggccgagct gaagatttca cgcgacggcc
     2221 aggagcaaat tgttaaaatt atcttaggag agtttgagga ataaaggttt ttttttgctg
     2281 actgaaataa taattaaaat tattaaagtt aaaagagtta ttgttgcgcc tattttttga
     2341 gaaaggcttt tggcggcttt aatttcaatc gtgtaatctc cggcaagatt tggttcaatc
     2401 aacattagat ccggaccaaa tttttttatt ggatattttt gattatttat aaaagcccgc
     2461 cagttggggt tgtaaaatga tttaaataaa agagcctgat ctgactggcc agcggtaacc
     2521 ttaatttgga ttaaatctgg ctggttgatg tcatagttta actggtaatt agcaggccct
     2581 ttttcaatcg ttttaaccag acccgccaaa tttttctgat ctaagacatt ggaaatggct
     2641 gaatactgtt taatattttt gaagtcaact agctggacca gatcagaatt gatcagatta
     2701 actgaataaa tattagagtc atcaattact tcttccagcc gcagctgatc aacaaattta
     2761 ttcggcctgg tgaaattctg atagcccata aacggcgtgg agacaagctg attattaaca
     2821 actatttttt ggacattgag cagtttcaac cagctgattg ctatccgggg atcttctccc
     2881 ttttcaatct gataaacaat atccgcccac cagcggctgg gctggacaaa atcctgggcg
     2941 cctttgattt gagctaagtc actaaaggca tttagccaaa atgagggcgg gccggttaaa
     3001 taaatttttt gaccggacag ctgacttgtc gtttcagtca aatattgagc gattttatat
     3061 tccgttgatt gagtaatatc cccgggtttg attactgttt gagccgatgg ccagaaatta
     3121 taaagggagg cggctaaaat aagggctaaa atcgcgggca aattatgttt aataataaat
     3181 ttttttgata gtttaaagct gtcatgcaaa taaagaccaa ttaagctgat taaggttatg
     3241 gcggccagat taaattcagg aacatatctt ttagcctggg gcaaaagcga ataatcaaaa
     3301 taaaaagcca gaaccgtaat cagccccaaa actacaaccg ctccggtaaa aaccaggctg
     3361 tgttgaagga gctgattttt tttgtattta ttttcaaaaa agtaaaaaaa gataaacaaa
     3421 gccagaatca ataaaatcca gattagcggc cagccggggc ttaaactgtt ttcccgatga
     3481 gcgtagccgc ttttaaaaat taattcaaaa aaataaggat tataatgaaa agcgatcagg
     3541 ccaagtatgg ccatcagcgc ccagaaagat gttttaaatt tgttttggga gttttttaaa
     3601 aataattctg aaattaaaat aatcaggcag attagggcca gagccagagc cccaaagaga
     3661 tcagtcaaca aaacagcgct ggctattaga ataaataaga taaaactttt aagacctcct
     3721 ttttttagta atttgatgta gacgatcagg cctagaggta taaaaaagag tgagataata
     3781 tgagggcctt ccccaaaaag cgtgtaaacc agcaaccgcc agggtaaaaa aatgttaaat
     3841 tcagccagag cgccttcaat tggcaggaac gaagccgaga cagccggacc gactgaataa
     3901 attaaagtca ggaagaaggg aattaaatta tttttagtaa gatatttgag taagatgaac
     3961 aaggaacagg ggattagaat ataaaataac ccggttataa tccgatagct ggcaaagatt
     4021 gacaagccct tgataaaaag agtcggcaat gaaattaaaa tcagacttaa aacaggataa
     4081 acaatttgtt tggtaaagcc ggtgtaccaa aaagggttga tgacattttg taaatttata
     4141 aaatagcctg aactaatagc gtgagaaaca gcgattgatt cggtgaattg gctgtgttca
     4201 ccctgaagca atgggaagac agcgactaag ttaattataa aaatgataat aatcagagca
     4261 gtcggcaaaa tgaatttttt ataatcaatc ataaaactta tttttggggt ctggctaaaa
     4321 ttatagcatc actatccaga tattttagct gccatttttc ttcagcaaaa agtttttggg
     4381 cggcgactga attgcgggcc agcaaagccc agtcaatatt gtaccggtcc aaaagatcaa
     4441 aattaaaatt gtgaattatg tcaaagtagt cctgataaat tgaccggttg ttttgtcgcc
     4501 agtggagcat ccggccgtca ataaaaactt tgtgttccgg cagaaaaaaa gttaaataac
     4561 tgccgtaatc atatttatta tacatttttc cctgaaattg atccgagttt gcctgaataa
     4621 atcggacagc gccctggggc agggtttttt gagcggcaat cactggccag tttaaactga
     4681 aactgatcgc gaaaatatta agaagcgcca gatagacaaa aggacttaaa ataattatca
     4741 gcggccagga attggtcagt tgtctgaata ttttggattt ttctgataat agactgaaac
     4801 tttccagata aatcgggact gaaaataaaa cgaagaaagg cgtgtgccgc cagctggata
     4861 aagccaacgg caaaaaaatg actgataaaa taatgtagag agaggacagt ttttttctga
     4921 agacaactgt taaaatcagg gttaaaatta aataaggcca gaagtagtaa gctcttaagc
     4981 cgttgaaact gaccggctgc cactcgctaa ttgttgcttt ggccagagaa ttgcccaggg
     5041 tgtcagcggc cgttttgaat atttccccgt aaatttgaaa gccatacgga ttaattaaag
     5101 ttattaaaac tgacatggct aaaagggctg ttaataattt aatttttctt ttttgaacag
     5161 tttcaaaaac gatcaaaata aagaggagac ccaggccgat aataaaaccg ccgtggagat
     5221 tggcccataa taataaaata aatgggatta aaaaaattat tttggggtta gtttgatagc
     5281 ggttataaat aaccaggatt aaagctaatc caaaaagggt gatcatttgc ggcttgggtc
     5341 caagaactga aaaaacagcc aggctggcca gagcggtagt cagcagcctg taaatccagt
     5401 tggttttaac caggccggtt attaaataaa atgttaaaag gagcatgaca gcgtaaaaaa
     5461 ttgttaaact gactaagccg aaccgggaat aaaggctgta aagaacaaca tcagtcagcc
     5521 attcgtgatt aacccagtta aagtcgggca ttgtctggga gaaaatgtca gtttggggaa
     5581 tagttttatt ttccagataa taacgcccgc tggccagatg ccagccagtg tcaaagctga
     5641 ctggcacccg ccaaaagact gaaaaaaccg taatcagcaa aaaaataatg attgtccagc
     5701 gccagatttt tgccatttga ttttaattaa aaaatttagt agaattagag aaattggaat
     5761 attatttagt ttaatttagt ttatcgctta tgactgaatt aatcaaagag atcaaagatt
     5821 tgcgaaaaaa aatctccggc acctggaggt tactttgacc tggataaaaa acaggttcaa
     5881 attaaaaatc taaccaggga agttgaacag ccggggtttt gggataatca ggaacaggca
     5941 aaaaaagttt ctaaagaact ggctgattcg caggcggaag tcagtcaatg gcagacaatg
     6001 aaagatcagg ctaaagaaat actggaactg gctgaactgg ctgaaaaaga aaaaactgac
     6061 ggtgatttgg aaaaagattt gcgaaagaaa ctggccaggc taagccagga gtttgaaaaa
     6121 ctggaatttt ttgttttatt ttcagacaaa tacgacagtc ataacgcgat tgtttcaatt
     6181 cacgccgggg cgggcgggac tgaagcccag gactgggccc agatgctgga gcggatgatt
     6241 ttacgatatt gtgaaaataa aaaatttaaa actgtcattg ctgaccaggc cgttgggagt
     6301 gaagccggca ttaaaagcgt tactttccgg gttgagggca aatattcttt tggttattta
     6361 aaatcagagg ccggcgttca ccgcctggtc agaatgtctc cgtttgacgc ggaaaaaatg
     6421 agacatacct ctttcgcgct gatcgaggtc ttgccggaac tggcagaaac tgatgaaaca
     6481 aaaattgatc ctaatgattt gagaattgat acttttattt catccggtcc cggcggtcag
     6541 ggaatgcaaa gatcttattc agccgtcaga ataacgcatt tgccaaccaa aacggtcgtt
     6601 acctgtcaaa atgaaaggtc ccaggtccaa aacaaggcaa ccgccatgaa aattttacag
     6661 tccaggcttc aacagcttca gcagtttgaa caggaaaaag aaaagaaaaa aatccggggc
     6721 gaatatctgt cagcccagtg gggcaatcag atcaggtcgt atgttcttca tccttataaa
     6781 atggttaaag accaccggac agattttgag gtaaaagaac ccgatgttgt tttggatggg
     6841 ggggtagatg gatttgtgga ggcttatctt agatgggtca agagatctgg taagttcaaa
     6901 gttagtaagt ccaaagttca aagttcaaag tccaaagttt cagccaaagg ctgatccgcc
     6961 tctggcggac aaagtccaaa gtttcagcca aaggctgatc cgcctctggc ggacaaagtc
     7021 caaggtttca gccaaaggct gatccgcctc tggcggacaa agtttaaatt tagtaagttt
     7081 aaagttagta aaaatatgaa aatattagtc accggcggcg ccggatttat tggctcccat
     7141 gttgttgacg ctttaatcgc caaaaaacat caggttgttg taattgatga tttatcaacc
     7201 ggcagaaagg agtttatcaa taaaaaagcc aaattttata agcttgatat tagaagtaaa
     7261 gagcttctta aagtttttaa aaaagaaaaa tttgccatag ttaaccatca cgccgctcaa
     7321 aaagatgtcc gcctgtcagt taaagatccg gtgtttgacg ctgaagtta
//
LOCUS       DASWHZ010000052        10862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1623232, whole genome shotgun sequence.
ACCESSION   DASWHZ010000052 DASWHZ010000000
VERSION     DASWHZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623232"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..371)
                     /locus_tag="VGA49_01210"
     CDS             complement(126..371)
                     /locus_tag="VGA49_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664420.1"
                     /translation="MIRLKMNNRLPSRSDGTAVIHPAVGGVYYLHAASSPRQAAGYSR
                     ADNKNSMINQKGNIYNKLIFVVTTLTFVIALSAIFLF"
     gene            complement(358..975)
                     /locus_tag="VGA49_01215"
     CDS             complement(358..975)
                     /locus_tag="VGA49_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664421.1"
                     /translation="MKRTLIFGLLILISLAGFSCKNNLVTDPNNYSSGDLKILREIRE
                     TEAWKLIQAPNGWIYYVSDNGKRYIFPNTGTFKSWFGEYQSEIKQASFEELAEIELGG
                     NVTYRPGYLIQTPTDPKIYWTSFNSQLRPFVSQDIVQEIYGKNWVDLAEELENFYFTN
                     YEIGEAIYQADLPTVSPDLTIDQDRSFSEPIRLDDQPVDTADDQT"
     gene            complement(1048..1575)
                     /locus_tag="VGA49_01220"
     CDS             complement(1048..1575)
                     /locus_tag="VGA49_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9664422.1"
                     /translation="MIIHILFLILYLIFTGFVFLGIFWFFTLAAMVSGAPYVPTSKSH
                     VREMVALAGVRPGELVIDLGSGDGRLLIESVRLGARGIGWEINPLLYFWSKFKIKQAG
                     FQDKIKINYGSFWKADISRADVIVLFLITHKMKKMKNKLQTELKPGTRVVSNGFKFPD
                     WEIINGNGAVYLYKI"
     gene            complement(1713..2273)
                     /gene="efp"
                     /locus_tag="VGA49_01225"
     CDS             complement(1713..2273)
                     /gene="efp"
                     /locus_tag="VGA49_01225"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9664423.1"
                     /translation="MISANDFKKGIIIIHNNEPWTIIDVQHTKVARGGGLYRTKIKNL
                     KTGNVLEVTFKSGEKVEEANISYQPVQYLYSDETSAFFMDPNYEQQSLDLATIGDRQK
                     YLKEGSSVDGLFLDDKFFDINLPKKMDFKVTQAPPGIKGDTATGGAKQVTLETGENIS
                     APMFINEGDIVRVNTETGEYVERVTE"
     gene            complement(2456..3118)
                     /locus_tag="VGA49_01230"
     CDS             complement(2456..3118)
                     /locus_tag="VGA49_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9664424.1"
                     /translation="MHTDSSGTIIYQAVGNHCAVDLTALVQELADKHNHKISKADALA
                     ILEVKGLRSVMVDFISIGRSLIGKAQHHRPARMAQAPDVVSCSSLTKYLYGLLGIWLP
                     RRAIQQKTFGSPVAITELQAGDLVFRRGLLRRTSFYDDPNHGIGHVGLSTGDKTIIHA
                     TNHKRGVVEDPVDQFTADKRYPRGACRIIPADSQIVTLKTLDYWEVETSDDIKWIILQ
                     SL"
     gene            3295..3711
                     /locus_tag="VGA49_01235"
     CDS             3295..3711
                     /locus_tag="VGA49_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX9664425.1"
                     /translation="MLNIATKSDYGLILMLELAKNYNSGYLSLTQIAKTKNLSANYLA
                     QLSIPLKQAGLVDSKEGKGGGYKLSKSPDQITVLEIVEAIEGPVNLVKCHKTENHDCQ
                     SFLACEAKMIWPAIFNEIKFVLRKKSLASLLATINH"
     gene            3716..4471
                     /gene="sufC"
                     /locus_tag="VGA49_01240"
     CDS             3716..4471
                     /gene="sufC"
                     /locus_tag="VGA49_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9664426.1"
                     /translation="MKNILEIKNLKVNLKEENKQILKGINLEIKPGEVHALMGPNGAG
                     KSTLAKVLFDHPSFELTDGRMIFDGREINALSTTERAQLGMFLAFQTPEELSGITVSS
                     FLRSAYNALRKAHDPDFKEVRAFQFKKILEEKMAELKFNPEFADRYINEGFSGGEKKK
                     AEILQLAVLEPKLAVLDEIDSGLDVDAMKLVSEGVNKLIKKGLSVLIVTHYQRILDYI
                     KPDYVHVIIDGQIAETGGPELAKKLETEGFKLD"
     gene            4572..5975
                     /gene="sufB"
                     /locus_tag="VGA49_01245"
     CDS             4572..5975
                     /gene="sufB"
                     /locus_tag="VGA49_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9664427.1"
                     /translation="MSHIFDKLNRETFDHKNKADYAKTAQVGLTENLVRQISAEKNEP
                     RWMLDIRLKGLELFDQLPLPDYGPDLSQLEFDKIIYYAKPADAKNTTSWDEVPEDIKQ
                     TFEKLGIPEAERQMLAGAGAQYEAATVYHNLKEQWTKQGVIFEDMDTAVQKYPDLVKK
                     HYMKQCVPVNLHKFTALHAAVWSGGTFLYIPKGVKVDQPMQAYFRMNAKNMGQFEHTL
                     IIIENEAEGHYIEGCSAPKYGSSSLHAGCVEIFVGDNARFRYSSVENWSRDTYNLNTK
                     RSLVGKNSFMEWVGGNLGSGVTMLYPCSVLKGEGGRADHLGIAFAGPGQNQDTGAKVI
                     HAAPRTTSNIVMKSLSKGDGLSVYRGLVHVAPQAENAKVNVNCDALLLSPKSKSDTIP
                     VMNIQNPKTQVAHEATTGKISEDKIFYLMSRGLSEGQAQGMVVNGFIEPIIKELPLEY
                     AVEMNRLISLEMEGSVG"
     gene            6140..7333
                     /locus_tag="VGA49_01250"
     CDS             6140..7333
                     /locus_tag="VGA49_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX9664428.1"
                     /translation="MKKTNFTNGYKDFLFQRLPESLKNVSLKEEAGFVIDAKKKAFNF
                     FEKQSLPRFKHEFLAKLNWPEQFKEIHPEITGLDQPGVTYYSAREAVEQTADLIKDKF
                     LALDYSLARDPLLAWLSYISRLAGLIVIDDKANLKQPVTILNNSKDSFLNYFIIIGGQ
                     DSQFEIVENNQLKNKTLSGQLIDLFLDKNSKVTYTAFDQNSQESQFLNYRRAQLNDQA
                     QIEWNNALFGSGLTKDFIASYLDHPRSRSEIKNVYLSKNNQNYYIDHHNWHRDAETKS
                     NMLSKGVLLDESLANYTGLIYMDGNSSQADGHLKSDTLLMGDKVINGNIPGLEIHNSQ
                     VQASHAATSSEVNPDHLFYCLSRGLTEKNAKQLMAEGLLASVYSQFHNQDIIEKIEKR
                     ISAKG"
     gene            7359..8660
                     /locus_tag="VGA49_01255"
     CDS             7359..8660
                     /locus_tag="VGA49_01255"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9664429.1"
                     /translation="MILTRISAKGGSASGGKNEFLFLKANKNLAYLDNAATTQKPLTV
                     INAISNYYKNENANIHRGVYQLSERATQKYEAVREKLAQFINAKEANEIVFTKGTTES
                     INFLAFSLGETFKKGDEIIISQGEHHSNLIPWQELAKRKKLTLKYIPVTRQGWLELNK
                     LDKLITKKTKLISITHISNVTGVINDIEKIIKKAHQKNVLVCVDAAQTAGHQKIDVQK
                     LNCDFLVFSGHKMYGPTGVGVLYGKKHLLEELPPYQTGGGMILQVNLSKTVYAGLPEK
                     FEAGTPNIAGVIGLGAAIDYLESVGFSKIKKRDQELTKYALGQLKKVKGLKFFGPASL
                     KNRSAIISFNVKGIHPHDLATIADQNNVAVRAGHHCAEPLHNLWNIPATVRISFGIYN
                     DKKNIDRLVKALNQAIKTFQKPEPNPYLSAEALSEGGETRK"
     gene            8665..9027
                     /locus_tag="VGA49_01260"
     CDS             8665..9027
                     /locus_tag="VGA49_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX9664430.1"
                     /translation="MNIYQEEILDHFQNPQNFGYLSDFDFEASLENPLCGDKIKIQVK
                     LDKDIIRDIKFSGQGCAISLATASMLTEEIKNKSISQVNHLTKDNITKLLNIELGPTR
                     LKCALLSLETIKKALAKK"
     gene            9220..9702
                     /locus_tag="VGA49_01265"
     CDS             9220..9702
                     /locus_tag="VGA49_01265"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX9664431.1"
                     /translation="MFKKLALIIIILVILGFGFVFYRAFTSFQPEAVVEQENVEQLNL
                     VEVENSALLRRGSFNEIDLIHKGGGQASVYQTGSGSILKLEDNFQVTNGPDLFVYLSD
                     QANIGPDKKDIGRFISLGRLQKPAGEQNYILPDNWSDYQSVVIWCRAFGVLFSAADLR
                     "
     gene            complement(9703..10113)
                     /locus_tag="VGA49_01270"
     CDS             complement(9703..10113)
                     /locus_tag="VGA49_01270"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX9664432.1"
                     /translation="MVNIWILPLAIVGFLVSYYIYEKKHDQETLVCLVGHACNEVVYS
                     KYGRMFGISNESLGLLYYATTALIVVASLFGLQALFGFSLNLIIFILTIPALVFSLIL
                     LFIMLFVLKTICEYCLLSSAATIGIFIIEFFALY"
     gene            complement(10117..10530)
                     /locus_tag="VGA49_01275"
     CDS             complement(10117..10530)
                     /locus_tag="VGA49_01275"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9664433.1"
                     /translation="MEFFTGLNQSGDWGLLALRLAVGAIFLAHGLSKRKMWKMRPSEQ
                     MPAGMLNQMKLLSIIEPLGGLAVLLGLFTQLGALALGIVMLGAIYMKKIKWQVPFTAS
                     DKMGWEFDLILLTACLLLLLMGAGGISLDSLLFGV"
     gene            complement(10547..10762)
                     /locus_tag="VGA49_01280"
     CDS             complement(10547..10762)
                     /locus_tag="VGA49_01280"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX9664434.1"
                     /translation="MEEKMSKKGEGLVCRCPHHKFVPGLVVLFGLLFLLANFGVFSAE
                     FVNVAWPILVLAAGLAKLSGSYCKCCC"
BASE COUNT         3382 a         2400 c         2186 g         2894 t
ORIGIN      
        1 aatattaggc aagtataaca aaaaattgcc atgcaaggca atccccgata acctattaat
       61 ctatcctccc gatcccgcct ttggcgggat cgtggtctct cgatccgcca taggcggatc
      121 ggagcctaaa acaaaaatat cgcgctcaaa gcaatgacaa acgttaaagt tgtcactaca
      181 aaaatcagct tattataaat atttcctttt tgatttatca tactattttt attatctgcc
      241 cgcgaatatc ccgcggcttg ccgcggggat gaagcggcgt gcagataata aaccccgccg
      301 acggcggggt gaataaccgc cgtcccgtca gagcgggacg gcaggcggtt attcatttta
      361 agtctgatca tcagcagtgt caaccggctg atcgtccagt ctaattggtt cggaaaaact
      421 gcggtcctgg tcaatcgtca gatcgggcga aaccgttggt aagtcggcct gatagatcgc
      481 ttcgccgatt tcataatttg taaaataaaa attttccagc tcttcagcca gatcaaccca
      541 gtttttgccg taaatttcct gaacaatatc ctgactgaca aaaggcctta gctgactatt
      601 gaaagatgtc cagtaaattt tagggtcggt cggggtttga atcaaatagc ccggccggta
      661 agtgacattg ccgcccaatt caatttcagc cagctcttca aagctagcct gtttgatttc
      721 agactgatac tcgccaaacc atgatttaaa agtaccggtg ttgggaaaaa tatatctttt
      781 gccgttgtcg gaaacataat agatccagcc gttgggcgcc tgaatcagtt tccaggcttc
      841 tgtctcgcgg atttcccgta aaatttttaa atcaccggaa ctgtaattgt tgggatccgt
      901 aacaagatta ttcttacagc taaaaccagc taaggaaatt aatataagta aaccaaaaat
      961 taatgttctt ttcataaatt tcttaaatct tataacttac aacttaaatc tgatccacca
     1021 aaggtggatc agcctctggc tgacaactta aatcttgtaa aggtaaaccg cgccattgcc
     1081 gttaataatc tcccagtccg gaaatttaaa accattggag acaactctgg ttcccggttt
     1141 taattcagtc tggagcttat ttttcatttt tttcatttta tgagtgatta aaaaaaggac
     1201 aatgacatcg gcccggctga tatcagcttt ccaaaaactg ccgtaattaa ttttgatttt
     1261 atcctgaaaa ccagcctgtt ttattttaaa ctttgaccaa aaataaagca aggggttgat
     1321 ttcccagcca atgcctctgg ctccgagcct gactgattca atcaacagcc ggccgtcacc
     1381 cgatcctaaa tcaatcacca gctcgccagg tctgaccccc gccaaagcaa ccatttcccg
     1441 gacatgactt ttgctggtcg gcacgtaagg cgcgccgctg accatggccg ccagggtaaa
     1501 aaaccagaaa atgccgagaa atacgaaacc ggtaaaaatt aaatatagta tcaggaataa
     1561 aatgtgaata atcatttgtt aatttttaat tcctaattga cctaatttct aatcaagctc
     1621 caattattaa atgacaaatg tcaaagaaat ttagttattg ggcattgatc attagacatt
     1681 ggctagaaat taaaaattag tcattagaaa ttttattccg tcaccctctc cacgtactcg
     1741 ccagtctccg tatttactct aacaatgtct ccctcattga taaacattgg cgctgaaata
     1801 ttttcgcctg tttcaagggt gacctgcttg gcgccgccgg tggccgtgtc gcctttaata
     1861 ccgggcggcg cctgggtaac tttaaaatcc atttttttag gtaaattaat atcaaaaaat
     1921 ttatcgtcca aaaacaggcc gtccactgaa gaaccttctt tcagatattt ttgccggtcg
     1981 ccaattgtcg ccagatcaag cgactgctgt tcatagtttg gatccatgaa aaaagcgctc
     2041 gtctcgtcac tgtataaata ctgaaccggc tgataagaaa tattggcctc ctcaactttt
     2101 tcgcctgact taaaagtgac ttccagaaca ttgccggttt tgagattttt aattttggtc
     2161 cggtaaagcc cgccgccccg ggcgactttg gtgtgctgaa catcaataat cgtccagggt
     2221 tcgttgttgt ggattatgat tattcctttt ttaaaatcat tggctgaaat catattattt
     2281 agattagatt atcacctgat aattcgttgg tcaactaact aaacaagggt gtcaaggatt
     2341 atcaggggat agcaagggat agcaaggatt atttgtattc cttgatttcc cctaatgtcc
     2401 cttgataaac cctgatatcc cctgaattaa aaaaacccgc atccaggatg caggtctaga
     2461 ggctctggag aatgatccac ttaatgtcgt cggaggtttc aacctcccag taatcaagtg
     2521 ttttcagagt gacgatctgg ctgtcggccg ggatgatccg acaggcgccg cgaggatatc
     2581 ttttatcagc tgtaaactgg tcaaccggat cttcaacaac accccttttg tggttagtgg
     2641 cgtgaatgat agttttatct ccggtggaca gaccaacatg accgatacca tggttaggat
     2701 cgtcataaaa gctggttctt ctgagtaaac cgcgcctaaa aaccaaatca ccggcctgaa
     2761 gttcagttat tgcgaccggc gagccaaaag ttttctgctg aattgcccgc cggggaagcc
     2821 agatacccag cagaccataa agatatttgg tcaggctgga gcaggaaacc acatcaggcg
     2881 cctgggccat tcgcgccggc cgatgatgtt gagctttgcc aatcaacgac cgaccaatgc
     2941 tgatgaagtc taccataacc gaccgtaaac ctttaacttc aagaattgcc aaagcgtcag
     3001 cctttgaaat cttatgattg tgcttatcag ccaattcttg aaccagcgcc gttaaatcaa
     3061 ccgcgcaatg gttgccaact gcctgataga tgatcgttcc gctgctatcg gtatgcatct
     3121 ggcatccctt cgtcaggatt ggattcaaaa aaagaacaaa aaatggcccg cgggacattt
     3181 agcttatagt ggcataattt gcaaaaatcc gcaactattg acatttcgcc aattatatac
     3241 tatcataaac agaaataaac tattatctgt ttgctatgag tttattttaa aattatgcta
     3301 aatatagcca caaaatcaga ctacggcctt attttaatgc ttgaattagc caaaaactat
     3361 aattcaggct atttatcatt gacccaaata gccaaaacca aaaacctgtc agccaactat
     3421 ctggcccagc tgtctattcc cctaaaacag gccggactgg tagacagcaa agaaggcaaa
     3481 ggcggcggtt ataaattgtc taaatccccc gatcaaatta ctgttctgga aattgtggaa
     3541 gcgattgaag gaccggttaa tctggtcaaa tgccacaaaa ctgaaaatca tgattgccaa
     3601 agttttctgg cttgcgaagc taaaatgatc tggccggcta tttttaatga aattaaattt
     3661 gttttaagaa agaaaagtct ggccagctta ctggcaacca ttaaccatta actttatgaa
     3721 aaatatttta gaaattaaaa atttaaaagt taatctaaaa gaagagaata aacaaattct
     3781 caaaggaatc aatctggaaa ttaaaccggg tgaagtccac gccctgatgg ggcccaacgg
     3841 cgctggcaaa agcactttgg ccaaagtttt gtttgaccac ccgtcttttg aactgactga
     3901 cggccggatg atttttgacg gccgggaaat taatgccctg tcaacaaccg agcgagccca
     3961 actgggaatg tttctggctt ttcagacgcc ggaagaatta agcggcatca ctgtttcttc
     4021 ttttttacgc agcgcttata acgccttgcg taaagcccat gatccagact ttaaagaagt
     4081 cagggctttt cagtttaaaa aaatcctgga agaaaaaatg gctgaactga aatttaatcc
     4141 ggaatttgcc gaccgctata ttaacgaggg tttttccggc ggtgaaaaga aaaaagcgga
     4201 aattttacag ctggccgttt tggaaccaaa gctggcggtg ctggatgaaa ttgattccgg
     4261 actggatgtt gacgccatga aactggttag tgaaggtgtt aataaattaa ttaaaaaagg
     4321 tctgtcggtt ttaattgtga ctcactacca gcggattctt gattatatta aacctgatta
     4381 tgttcatgtc attattgacg gccaaatcgc tgaaaccggc ggcccggaac tagctaagaa
     4441 attagaaact gaaggtttta aattagacta aatccccttt acccccccgc caggcgaccg
     4501 cgaagcggtc ttggcggggt cccgccatag gcgggattta tcccccttaa cccctttaac
     4561 ccccttattt tatgtcccat atatttgata aacttaacag agaaaccttt gaccacaaaa
     4621 acaaagctga ctacgctaaa actgctcaag tcggcctgac tgaaaatttg gtcagacaaa
     4681 tttccgcgga aaaaaatgaa ccgcggtgga tgcttgatat tagattaaaa ggtttggagc
     4741 tgtttgatca gctgcctctg cctgactatg gtccggattt aagccagctg gagtttgata
     4801 aaattattta ttacgccaaa ccggccgacg ccaaaaacac cacttcctgg gatgaggtgc
     4861 ctgaagacat taaacaaact tttgaaaagc tgggcattcc cgaagctgaa cggcagatgc
     4921 tggccggcgc cggcgctcag tacgaggcgg ccactgttta tcataacctg aaagaacagt
     4981 ggactaaaca aggagtgatt tttgaagaca tggacacggc cgttcagaaa tatcctgatc
     5041 tggtcaaaaa acattatatg aaacagtgcg tcccggttaa tcttcataaa ttcacggcgc
     5101 tccacgccgc tgtctggagc ggcgggacat tcttatacat tcccaagggc gttaaggttg
     5161 accagccaat gcaggcttat ttccggatga acgccaaaaa catgggccag tttgaacata
     5221 ctttaattat tattgaaaac gaagctgaag gccattatat tgaaggctgt tcggctccca
     5281 aatacggctc aagttcattg cacgccggct gtgttgaaat ttttgttggc gacaacgccc
     5341 gttttcggta ttccagcgtg gaaaactgga gccgggacac ttacaattta aacaccaaga
     5401 gatcgcttgt cggcaaaaat tcattcatgg aatgggtcgg cggcaatctg ggcagcggcg
     5461 tcactatgct ttatccctgc agtgtcttaa aaggcgaagg cggccgggct gatcatctgg
     5521 gaattgcttt tgccggcccg ggccaaaacc aggataccgg cgccaaagtc attcacgccg
     5581 cgccccggac aacttccaac attgtcatga aaagtttgag caaaggcgac ggcttgtctg
     5641 tttaccgggg tttggttcat gtcgctcccc aggccgaaaa cgccaaagtc aatgttaact
     5701 gcgacgcctt actgttaagc cccaaatcaa aatcagacac tattccggtg atgaatattc
     5761 aaaaccccaa aacccaggtg gctcatgaag cgaccaccgg gaaaatcagt gaagataaaa
     5821 tattctattt aatgtcgcgc ggtttgagcg aaggccaggc ccaggggatg gtcgtcaacg
     5881 gctttattga gccaatcatc aaagaactgc cgctggagta cgccgtggaa atgaaccggt
     5941 tgatcagttt agagatggaa ggctctgtag gataagaaaa agatatgggt gttaaaaaat
     6001 ttgctctgtg cgccaaaggg gtctggggta gccttgagcc cattgcaaaa gctgattata
     6061 aaaatagcac actggcgaat tggctacccc agggcttttt gctacttttt ggccccaaaa
     6121 agtagataat ctgaatttta tgaaaaaaac taattttaca aatggttata aagatttcct
     6181 ctttcagcgc ttacctgaaa gtttaaaaaa tgtttcttta aaggaagaag ctggttttgt
     6241 tattgatgct aaaaagaaag cttttaattt ttttgaaaaa caatctctgc caagatttaa
     6301 acatgaattt ttggctaaat taaactggcc ggaacagttt aaagaaatcc atccggaaat
     6361 taccggcctt gaccagccgg gcgttaccta ttattccgcc cgggaagccg ttgaacaaac
     6421 agccgattta attaaagata aatttttagc tctggactac tctttagccc gcgatcctct
     6481 gttggcctgg ctgtcttata ttagccggtt ggccggttta attgttattg acgataaagc
     6541 taaccttaaa cagccagtta ctattcttaa caactctaag gattcttttc tgaattattt
     6601 tattattatc ggcggtcagg acagccagtt tgaaattgtt gaaaacaatc agcttaaaaa
     6661 taaaacttta agcggccagc tgattgatct gtttctggat aaaaattcta aagtaactta
     6721 caccgctttc gatcaaaatt ctcaagaatc ccaatttctg aattacagac gagctcaact
     6781 taatgatcag gctcaaatcg aatggaataa tgctttattt ggcagcggcc tgaccaaaga
     6841 ctttattgcc agctatctgg atcacccccg aagccggtct gaaattaaaa atgtttatct
     6901 gtcaaaaaat aatcaaaatt attacattga ccaccacaac tggcaccgcg acgctgaaac
     6961 caaatcaaat atgctaagca agggcgtttt gctggatgaa tctttggcca actacaccgg
     7021 cctgatttat atggatggga actctagtca ggctgacggt catctcaaat cagacacttt
     7081 gctaatggga gataaggtga tcaatggcaa tattcccggg ctggaaattc acaacagtca
     7141 ggttcaggct tcgcatgccg ccacctcaag cgaagtcaat cctgatcatt tattctactg
     7201 cttgagccgg ggcttaaccg aaaaaaacgc caaacagctg atggccgaag ggttgctggc
     7261 cagtgtttac agccagttcc ataatcagga tataattgaa aaaattgaaa agcgaatttc
     7321 agccaaaggc tgatcaacct tggttgaaaa aactaattat gattttaacc cgaatttccg
     7381 ccaaaggcgg atccgcctcc ggcggaaaaa acgaatttct ttttctaaaa gctaacaaaa
     7441 atttagctta tcttgataat gccgccacga ctcaaaaacc tctaacagtt attaatgcca
     7501 taagtaatta ctacaaaaat gaaaacgcca atattcatcg gggcgtttat cagctaagcg
     7561 agcgagccac tcaaaaatat gaagctgtcc gggaaaagct ggctcaattt atcaacgcca
     7621 aagaagccaa tgaaattgtt tttaccaaag ggacgactga atcaattaat tttctggcgt
     7681 ttagtctggg tgaaactttt aaaaaaggcg atgaaattat catcagccag ggcgaacacc
     7741 acagcaacct aatcccctgg caggaactgg ctaaaagaaa aaaattaact ctaaaatata
     7801 tcccggtcac caggcaaggc tggttggaac tgaataagct tgataaatta attaccaaaa
     7861 aaactaaatt aatttctatt acccatatct caaatgtcac cggcgtcatt aatgatattg
     7921 aaaaaataat taaaaaagcc catcaaaaaa acgttttagt ctgcgttgac gccgctcaaa
     7981 ccgccggtca tcaaaaaatt gatgttcaaa aactgaactg cgattttctg gtttttagcg
     8041 gccataaaat gtacgggcca acaggcgttg gtgttttgta cggcaaaaaa catttactgg
     8101 aagaactgcc gccttatcaa accggcgggg gcatgatttt acaggttaac cttagcaaaa
     8161 ctgtttatgc cggcctgcct gaaaaatttg aggccggcac gcccaatatc gccggcgtca
     8221 tcggcctggg cgcggccatt gattatctgg aatcagtcgg ttttagtaaa attaaaaaac
     8281 gcgatcagga attaaccaaa tacgccttgg gccaattgaa aaaagttaaa ggcttaaaat
     8341 tctttggtcc ggccagtttg aaaaaccggt cagcgattat ttcatttaat gttaaaggga
     8401 ttcatcccca tgatctggcg acaatcgccg atcaaaataa tgtcgccgtc cgggccggcc
     8461 accattgcgc cgaaccactc cataatttat ggaatatccc ggccaccgtc agaatcagct
     8521 ttggcattta caatgacaaa aaaaacattg accgcttagt caaagcgctt aaccaggcaa
     8581 taaaaacttt ccaaaaacca gaacctaacc cctacttgtc cgccgaagcc ttaagcgaag
     8641 gcggagaaac tagaaaataa aagtatgaat atttaccagg aagaaatttt agatcatttt
     8701 caaaaccctc aaaatttcgg atatttgagt gattttgact ttgaagccag cctggaaaat
     8761 cctttatgtg gcgataaaat caaaattcag gtaaaattag ataaagacat catcagggat
     8821 attaagttca gcggtcaggg ctgcgccatc agcctggcga cggcttcaat gctgactgaa
     8881 gaaattaaaa ataaatcaat tagccaggtt aatcacctga ccaaagataa tattaccaaa
     8941 ctgttaaata ttgagctggg gccaacccgc ctaaaatgcg ctttgctgtc tctggaaaca
     9001 atcaaaaaag ctttagccaa aaaataattc ccctctaaaa aaatccaaat ttcaaaatcc
     9061 caatgacaaa tcaaatccaa aatccaaagc tcaaaaataa ttttgtcatt tagattttgg
     9121 atttcaattg atatttgagc tttgaaattt gttatttcac tcccttatct ccgctgataa
     9181 tccttaataa cccgttgtca cctataaccc ctttataaaa tgtttaaaaa gcttgctcta
     9241 attattatca tactagttat cctgggtttc ggttttgtct tttaccgggc ctttacctca
     9301 tttcagccgg aagcggttgt tgaacaggaa aatgttgaac agcttaacct ggttgaggtt
     9361 gaaaactctg ctctgctccg aaggggcagc tttaatgaaa ttgatctgat ccacaaaggc
     9421 ggcggccagg cttcagttta tcaaactgga tctggttcaa ttttaaaact ggaagacaac
     9481 tttcaggtaa ccaacggacc tgacctcttt gtttatttat cagatcaggc aaatattggt
     9541 ccggataaaa aagacattgg caggtttatt tctttgggcc ggctgcaaaa accggcaggt
     9601 gaacaaaatt atattttacc tgacaactgg tcagattatc agtcagtagt tatctggtgc
     9661 cgggcttttg gcgttctctt cagcgcggct gatctgcgat aattaataca acgcaaaaaa
     9721 ctcaataata aaaatgccga tggtggccgc ggaacttaac aggcagtact cacaaattgt
     9781 cttaagaaca aacagcataa taaacagcag gattaatgaa aaaaccaaag ccgggattgt
     9841 caggataaaa ataatcagat ttaaagaaaa tccaaacaaa gcctgcaaac caaaaagtga
     9901 cgccacgact atcaaagcag tggtcgcgta atagagcaat cctaaacttt cattcgaaat
     9961 gccaaacatt cggccgtatt tactgtagac aacttcattg caagcatgcc caaccaggca
    10021 aaccagcgtt tcctgatcat gcttcttttc ataaatataa taagaaacca aaaacccaac
    10081 tatcgccaaa ggtaaaatcc agatattaac cataaattaa acgccgaaca ataaactatc
    10141 caatgaaatt ccaccggcgc ccatcagcaa taaaagcaga caagccgtca gaagtatcag
    10201 atcaaattcc cagcccattt tgtcactggc tgtaaaagga acctgccact ttattttttt
    10261 catgtaaatc gcccctaaca tgacaattcc taaagccaaa gcgcctaatt gagtaaataa
    10321 acccaacaaa acagccagcc cgcccaatgg ttcaatgatt gacaacagtt tcatttgatt
    10381 gagcatgccg gcgggcattt gttcagacgg ccgcattttc cacatttttc ttttagacaa
    10441 tccgtgggcc aaaaaaatcg ccccaacagc caagcgcaaa gccaataaac cccagtcgcc
    10501 agactggttc aaacctgtaa aaaattccat atatttgaga acttaattaa caacagcact
    10561 tgcagtaaga accgcttaat ttagccagac cggccgccag aaccaggatt ggccaggcga
    10621 cattaacaaa ctcggcgcta aaaacgccaa aattagccaa caaaaaaagc agtccaaata
    10681 gaactaccag gccgggcacg aacttgtggt gagggcagcg gcaaaccagc ccttcccctt
    10741 tcttggacat tttttcttcc atatgcatat gatatacgtt aattattaac aaagtcattt
    10801 ataattatag catattctta aaaatcttgc agtctggata ctttttggct aaggtattaa
    10861 ta
//
LOCUS       DASWHZ010000053         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3104561, whole genome shotgun sequence.
ACCESSION   DASWHZ010000053 DASWHZ010000000
VERSION     DASWHZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104561"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VGA49_01285"
     CDS             complement(<1..416)
                     /locus_tag="VGA49_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664435.1"
                     /translation="MPYSRSPQNWRRKRFRPNRPDHPSEKKTDFHPVFTTLKKIIRGL
                     KNLFLFLFFKTFSLIKAARKRNIKYALQLTIVAGIIVFLLGGIIGVAALAYYSKDLPE
                     PDKLIDRSVAQSTKIYDRTGENLLYEIHGDQNRTII"
     gene            577..813
                     /locus_tag="VGA49_01290"
     CDS             577..813
                     /locus_tag="VGA49_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664436.1"
                     /translation="MDQRKRDQAINEYLEWGVCEFFGEEIDLSDFLEQFANPDEKLIR
                     HLRDCRHCRKLYLAHGFELPEGVEPSSEDITMFD"
     gene            1114..1299
                     /locus_tag="VGA49_01295"
     CDS             1114..1299
                     /locus_tag="VGA49_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664437.1"
                     /translation="MSSHNTKALHCPECKKFKLLPRGQEWLEKRELMASQQQAIDEGN
                     ARLKTMICPDCRKTSGL"
     gene            1483..1707
                     /locus_tag="VGA49_01300"
     CDS             1483..1707
                     /locus_tag="VGA49_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664438.1"
                     /translation="MNWLAEFFRKIFLDRWLAGEKAKEEEVGQPIITPEMIKAIKAGE
                     ICHCGGDWRKLEPCEYVCIRCGSRMTKAGG"
     gene            complement(1892..2386)
                     /gene="ruvC"
                     /locus_tag="VGA49_01305"
     CDS             complement(1892..2386)
                     /gene="ruvC"
                     /locus_tag="VGA49_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9664439.1"
                     /translation="MPRLKQIILGIDPGVATTGYGIIEKIVSQLTLIDYGCILTKAKT
                     PLPERFQTIHQELKKIIKKYNPSVVAVEELFFFKNVKTALIVGQARGVVLLTAIQKNL
                     PVYEYTPLQVKQATTTYGRADKQQVQQMVKAILNLKEIPKPDDAADALAVAICCANSL
                     GDGV"
     gene            complement(2503..3225)
                     /locus_tag="VGA49_01310"
     CDS             complement(2503..3225)
                     /locus_tag="VGA49_01310"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9664440.1"
                     /translation="MSGHSKWSKIKRQKGAADAKRSALFTKLGNAISLAAREMGGDLE
                     TNFKLRLAVDKARSANMPRENIDRAIKRGAGELAGDQIEEIIYEGYGPLGVAFVIEAL
                     TDNKNRTVGSVRHILAKHDGNLGGSTLWLFDRKGVIRIDPANLKMDKDEFLLKAIDLG
                     TEDVQEDEDSLIIYTDPGDLQKVKELLEKEGLEIEYAELEYVAKDQIKVVDETLKNSL
                     QKIYVELDEDPDVSNYYTNTDY"
     gene            3418..4491
                     /locus_tag="VGA49_01315"
     CDS             3418..4491
                     /locus_tag="VGA49_01315"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX9664441.1"
                     /translation="MILDDLKKIKKLDSKGMIESIEHIYRQCQQAVEEVRKIKIPRSY
                     AVASDIIVFGVGGSALGPRVAQTLFADSLKLPIEIVRDYSLPAYLGKNSLVILSSYSG
                     NTEEVLIAAQEVSRRTKKIMAITSGGQLKNLARRKKWPVYLIDPVYNPCGQPRMAIGY
                     SIMGIVKLLEKTGLIKLSDQEVKKMISYVAGLNSRFGLKVKKNNLAKKTALELHNKIP
                     IFIASEHLVGNANVAANQINENGKNLAMFFTIPEINHHLLEGLSFPKQSKKSFYFIFL
                     NSRFYHPRTQLRYKITMDILKKRGYEFTEIKLTGPTKLIQSFETLSLTSYLGFYLAML
                     NKIDPSPIPWVDYLKKAIGSDPP"
BASE COUNT         1255 a         1002 c         1086 g         1377 t
ORIGIN      
        1 ctgataattg tccggttttg atcaccgtgg atttcataca gcaggttttc gccggtccgg
       61 tcgtagattt tggtgctttg ggcgactgac cggtcaatca gcttatctgg ctcgggcagg
      121 tctttgctgt aataggccag cgcggcgacg ccaatgatgc cgcccagcaa gaagacaata
      181 atgccggcga caattgttag ttgcagggcg tatttaatat ttctttttcg ggcggccttg
      241 atcaaagaaa aagttttaaa aaataaaaaa agaaaaagat ttttcaatcc cctgataatt
      301 ttttttaatg tggtgaaaac cggatggaaa tcagtttttt tttctgaagg gtggtcaggg
      361 cgattgggtc tgaagcgttt ccttctccag ttttgcggcg aacgactgta aggcatactt
      421 ttttaaactc ttttataata gagaatttaa ggggttttgt aaaggcctta taaaattata
      481 ccaaatttag tttgacataa ccaaaacaat catctatatt aaaaaatcgt tcgtgtttgt
      541 ttttaaccgc cggccgcgtg aaagggatgg gcaacaatgg atcagcgaaa acgggatcag
      601 gcaatcaacg aatatctgga atggggggtt tgtgagtttt tcggagaaga gatcgatctg
      661 tcggattttc tggaacagtt cgctaacccg gacgaaaaac tgatccggca tcttcgtgac
      721 tgccgccact gtcgcaagct ttatctggcg catgggtttg aactgccgga gggggtagag
      781 ccgtcgagtg aagacattac gatgtttgat taggggctga caaaaaatag gaggctacag
      841 tgtgagcgaa ggctcgcact ttttttatgc cccgccacgg cggggcgggc aaaaggcacg
      901 aggcaatagg caaaagggtt ggtgttcccg ccttgggcgg gggacaatta tttgattggc
      961 ataaggtaaa agggttttta ctaatgcctg atccctgttg cctagcctat tgccttgtgc
     1021 ctattgcctt gtgccttttt ggaattgaca aaaatacaaa actgtgataa atatagggtg
     1081 gaagttttgt ttgttccctg aaaggaggga aagatgtcgt cccataacac caaagcgctt
     1141 cattgcccgg agtgtaagaa attcaagctt cttcccagag gacaggagtg gctggaaaag
     1201 agggagttga tggcttcaca acaacaagcc attgacgaag gcaacgccag gctgaaaacc
     1261 atgatttgtc ccgattgccg gaaaacatcg ggactgtagg gggtccggca tggttgggaa
     1321 aatacaaccc atcgtcttgt tccgctgtcc gtgctgcgac cggtgccggc tgaatgagga
     1381 ataccgctgg catcggatga gcctgaccat ccttgaccta attaataaaa aaaaggttga
     1441 ggttaaagaa caggtttgcc cgggatgttg tcaggagaaa tcatgaactg gctggccgaa
     1501 ttttttcgaa agatcttttt ggacagatgg ctggctggtg aaaaagcaaa ggaagaagaa
     1561 gtcggccaac caatcattac ccccgagatg attaaggcca tcaaggctgg tgaaatttgt
     1621 cattgcgggg gagattggag aaaactagag ccgtgcgagt atgtttgtat ccgatgcggc
     1681 tcaaggatga caaaagccgg aggataaaaa gctccatttt gcggaagcag gatggagctt
     1741 tctcattttt tctatcccct atcccctata ccctatcccc tatcccctat tccctatccc
     1801 ctatccccta ttccctatcc cctatcccct attccctatc ccctatcccc tataccccat
     1861 cccctatccc ctattcccta ttccctatcc cctatacccc atctcctaaa ctattggcgc
     1921 agcagatggc tacggccaaa gcgtcggcgg cgtcgtccgg cttgggaatt tcttttaaat
     1981 taagaatagc tttgaccatc tgctggactt gttgtttgtc agcccggccg taggtggtcg
     2041 tggcttgttt aacctggagc ggggtgtatt cataaacagg caagtttttt tgaatggccg
     2101 ttaaaaggac cacgccccgg gcctggccga cgattaaagc ggttttaaca tttttaaaaa
     2161 agaacagttc ttcaaccgcg acaactgacg gattatattt tttgataatt ttttttagtt
     2221 cctgatgaat tgtttgaaat ctttccggca aaggagtttt agctttggtc aaaatacagc
     2281 cgtagtcaat tagtgttaat tgactgacta ttttttcaat aatcccatag ccagtggtgg
     2341 ctacgccggg gtcaatgccg aggatgattt gttttaatct gggcataagg ataaaagtaa
     2401 aaagtaaaag gtcaaaagta agaagtaaaa gttaaaagtg aaaagttttt aaaaataaaa
     2461 taacttctta attttttact ttttacttat tacttttaac ttttaataat cagtgttcgt
     2521 ataataatta ctgacatccg ggtcttcgtc cagctcaacg taaatttttt gcaaactgtt
     2581 tttcagcgtt tcgtctacaa ctttgatctg atctttggcg acatattcaa gttcggcgta
     2641 ttcaatttcc aaaccttctt tttccaacag ctccttgacc ttttgtaagt ctcccggatc
     2701 agtgtaaata atcaggctgt cttcgtcttc ttgcacatct tccgttccca gatcaatcgc
     2761 cttcaggaga aactcatctt tgtccatttt taaatttgcc gggtcaattc taatgacgcc
     2821 ctttcggtca aacagccaga gggtgctgcc gcccagatta ccgtcgtgtt tggccaaaat
     2881 atgccggacg gagccaactg tccggttttt attgtcagtc agggcttcaa ttacaaaagc
     2941 cacgcccagg gggccatagc cttcataaat aatttcttca atctggtcgc cggccagctc
     3001 gccggcgccg cgcttgatcg cccggtcaat attttcccgg ggcatgttgg ctgaccgggc
     3061 tttatcaacg gctaagcgca gtttaaaatt agtttccaga tcgccgccca tttcccgggc
     3121 ggccaggctg atggcattgc ccagttttgt aaaaagagcg ctccttttgg cgtcagccgc
     3181 tcctttttgt cttttaattt ttgaccattt gctgtgaccg gacatagcaa gcaaggttta
     3241 tcagggttga taaagggata tcaggggaaa tcagggatta tccttgatac accttgctat
     3301 cccttgataa tccttgataa ctcttgttct ttataccaaa aaaggtttta aaaatcaatc
     3361 atttcaggta taattattga aaaccaggat taagaattaa gcagtaaaaa ttaagctatg
     3421 attttggatg atttaaagaa aataaaaaaa ctggattcaa aaggaatgat tgagtcaatt
     3481 gaacatattt atcggcagtg ccagcaggcc gtggaagagg tcagaaaaat taaaattccc
     3541 agatcgtatg ccgtggctag cgacataatt gtttttggcg ttgggggttc ggctctggga
     3601 ccgcgtgtcg cccagacatt gtttgccgat tctttaaaac tgccaataga aattgtccgc
     3661 gactacagcc tcccggctta tttgggaaaa aacagcctgg tgattttatc cagctattcg
     3721 ggcaatactg aagaagtctt aattgccgcc caggaagtca gccggcggac gaaaaagata
     3781 atggctatta cttccggcgg tcagttaaag aacctggccc ggagaaaaaa gtggccggtc
     3841 tatttgattg atccggttta taatccctgc ggccagccgc gaatggccat tggttattca
     3901 attatgggaa ttgttaaatt actggagaaa accggtttaa taaaattgag tgatcaggaa
     3961 gttaaaaaaa tgatcagcta tgtcgccggc ctgaacagcc gctttgggct gaaagtcaag
     4021 aaaaataatc tggccaaaaa gaccgccctg gagcttcata ataaaatacc aatttttatt
     4081 gcttccgagc atttggtcgg caacgccaat gtggccgcca atcaaattaa tgaaaacggc
     4141 aaaaatttgg cgatgttttt tactattcct gaaatcaacc accatttgct ggaagggttg
     4201 agttttccga agcagtctaa aaaaagtttt tattttattt ttttaaattc aagattttat
     4261 catccccgga cacagcttcg ttataaaatc accatggata ttttgaagaa gcgggggtat
     4321 gaattcacgg aaatcaaatt gaccgggccg actaagctga ttcagtcttt tgaaactttg
     4381 agcctgactt cttatctggg gttttatctg gcgatgctca ataaaattga tccgagcccg
     4441 attccctggg tggattattt aaagaaggca ataggttccg atccgccata ggcggatcga
     4501 gaatcctcga tcccgccttt ggcgggatcg ggacacaagg gttggtgtcg ccacgccagg
     4561 ctgttgcctg gcttcgcgac ttattttttt atgcatcgat tacagctgtt ttttaattct
     4621 catcatttcc tcgtttattt gattgttttt ataattgcct tctctctttt caccctgctt
     4681 caagcttccc cgacccttgc tgatccagat tctttttatc
//
LOCUS       DASWHZ010000054         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_125032, whole genome shotgun sequence.
ACCESSION   DASWHZ010000054 DASWHZ010000000
VERSION     DASWHZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125032"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VGA49_01320"
     CDS             complement(<1..891)
                     /locus_tag="VGA49_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9664442.1"
                     /translation="MDILEGLNQEQQEAVSHDQGPLLIIAGAGTGKTTVITRRVAWLI
                     DQALARPDEILALTFTEKSAGEVEERVDKLLPYGYVDLWISTFHSFCERILQSHALDI
                     GLPNQFKVFDQTGQWLLVRKNLDKFSLNYYRPLGNPTKFIHALLEHFSRCKDEEVYPE
                     DYLKHAEELILNSDSAEFIRSNTNLSKKEIKEALTAEVKRTEEIADAYHAYQQLLLDN
                     DALDFGDLINYCLKLLRERPLILGKYRQQFKYILVDEFQDTNWAQYELVKLLAGSKNN
                     LTVCGDDDQSIFRFRGASISN"
     gene            complement(900..1319)
                     /locus_tag="VGA49_01325"
     CDS             complement(900..1319)
                     /locus_tag="VGA49_01325"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9664443.1"
                     /translation="MSHICQALILSCIDFRFVSAIHRFAEAEGLEGNYDYAALAGGSM
                     DILEKSGQSIIINQIDIASRLHQIKKIYLIHHEDCGAYGGKKSFSSDEEELTKHKTDL
                     QKTTAVIKNKFPDLEIINVFAHLKEEGGQNVIWFEKL"
     gene            complement(1321..1743)
                     /gene="atpC"
                     /locus_tag="VGA49_01330"
     CDS             complement(1321..1743)
                     /gene="atpC"
                     /locus_tag="VGA49_01330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX9664444.1"
                     /translation="MKINFEITTPEKIAHKDQIDSVTVPTKMGQITILPNHIPLVSSL
                     VAGELIVRKDGQETPLAVSGGFVNVQPNNRVVILADTAEKVEEIDEELAERARQRAAE
                     LLKTRREDAEDYAVILGELNKEMARLKLLRKYRRLKTK"
     gene            complement(1776..>2408)
                     /locus_tag="VGA49_01335"
     CDS             complement(1776..>2408)
                     /locus_tag="VGA49_01335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121709.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9664445.1"
                     /translation="IFRFTQAGSEVSALLGRIPSAVGYQPTLASEMGQLQERITSTKK
                     GSITSVQAVYVPADDLTDPAPATTFAHLDSTIVLARSLAELGIYPAVDPLDSTSTILD
                     PQIVGEEHYNVAREVQRVLQRYKELQDIIAILGMEELSDEDKLTVARARKIQKFLSQP
                     FFVAEAFTGLAGQYVPLAETIKGFKEILDGKHDDKPEQAFYMKGSIDQLK"
BASE COUNT          666 a          561 c          472 g          709 t
ORIGIN      
        1 attactaatt gacgcccccc gaaatctaaa aattgactgg tcatcatcgc cgcagaccgt
       61 caaattattc tttgagccag ccagtaattt aaccagttca tactgggccc agttggtatc
      121 ctggaattcg tcaactaaaa tatatttaaa ttgctggcga tattttccca aaattaaagg
      181 ccgttccctt aaaagcttta aacaataatt aattaaatcg ccaaagtcca aagcgtcatt
      241 atccaaaagt agctgctgat aagcatgata agcatcggca atttcctctg ttcttttaac
      301 ttcagcggtc aaagcttctt taatttcttt tttggataaa ttggtattac ttcgtataaa
      361 ttcagccgag tcgctgttta aaatcagctc ttcagcgtgt tttaaataat cttcaggata
      421 aacttcctca tctttgcagc gggagaaatg ttccagtagg gcgtgaataa atttggttgg
      481 attgcccaga ggccggtagt agtttaaact aaatttatcc aaattttttc tgaccaacaa
      541 ccattggccg gtctgatcaa aaactttgaa ctgattaggc aagccaatat ccagagcgtg
      601 gctctgaaga attctttcac aaaaagaatg aaaagttgaa atccataaat ccacgtatcc
      661 ataaggcaat aatttatcaa cccgctcttc aacttctccg gccgattttt cagtaaaggt
      721 caaagccaaa atctcatccg gcctggccag agcctggtca atcagccagg caactcttct
      781 ggttatcacg gttgttttgc cggtgccggc tccggcaata attaaaagag gcccctgatc
      841 atggctgacc gcctcttgtt gctcctgatt taatccttct aggatgtcca tagtacctcc
      901 tacaatttct caaaccaaat cacattctgg ccaccttctt ctttaaggtg agcaaaaaca
      961 tttataattt ctaaatcggg aaatttattt ttaatgacgg ctgttgtttt ttgaagatca
     1021 gttttatgtt tagtcaattc ttcttcgtct gaactgaaac tttttttgcc tccataagcg
     1081 ccgcagtctt cgtggtggat caaataaatt tttttaatct gatgaagccg gctggcaata
     1141 tcaatttgat ttataataat actttgcccg cttttttcca gaatatccat tgagccgcca
     1201 gccagagccg cataatcata attgccttcc agcccctcgg cttcagcgaa ccggtgaata
     1261 gcgctgacaa atctaaaatc aatgcaggat aatattagag cttggcaaat atgactcata
     1321 ttatttagtt tttaatcttc ggtattttct cagtaatttt aatcgggcca tttctttgtt
     1381 aagttcgccc agaataacag cgtaatcttc agcgtcttcc cgcctggtct ttagtaattc
     1441 agccgctctt tggcgggccc gttcagccag ttcttcatca atctcttcca ctttttcagc
     1501 ggtgtcagcc agaataacca cccggttatt gggctggaca ttaacaaacc cgccggaaac
     1561 cgccagcggt gtctcctggc cgtcttttct gacaatcaat tcaccggcca ccagggaaga
     1621 aactaaagga atatgattag gcaaaatagt aatctgcccc attttggtcg ggacagtcac
     1681 tgaatcaatc tggtccttat gggctatttt ttccggagtg gtaatttcaa aattaatttt
     1741 catacgtcag acccgtcgct ggctggcgac cgggtttatt tcaactgatc aatagagcct
     1801 ttcatgtaga aagcttgttc tggtttatca tcgtgcttgc cgtccagaat ttctttaaag
     1861 cctttgatag tttcggctaa ggggacatat tgtccggcca gaccggtgaa agcttcagcc
     1921 acaaaaaaag gctgggataa aaacttctga atttttctgg cccgggcgac tgtcagctta
     1981 tcctcatcag acagttcttc cattcccaaa atagcaataa tatcctgaag ttctttatag
     2041 cgctgcaaga ctcgctgaac ttcccgggca acattgtaat gttcttcgcc aacaatctgc
     2101 ggatcaagaa tggttgaagt cgaatccaaa ggatcaaccg ccggataaat acccagttca
     2161 gccaaacttc tggccaagac aatagttgaa tccagatgag caaaagttgt ggccggagcc
     2221 ggatcagtca ggtcgtcagc cggcacataa accgcctgga ctgaagtaat tgaccctttt
     2281 ttggtggaag taattctttc ctgaagctga cccatttccg aagccaaagt cggctggtag
     2341 ccaacagcgg acggaatccg gcccagcaaa gccgagactt cggaaccagc ctgagtaaac
     2401 cggaaaat
//
LOCUS       DASWHZ010000055         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1237792, whole genome shotgun sequence.
ACCESSION   DASWHZ010000055 DASWHZ010000000
VERSION     DASWHZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237792"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGA49_01340"
     CDS             complement(<1..396)
                     /locus_tag="VGA49_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664446.1"
                     /translation="MTGKKILLITFVSLFALNLLFGFGVLTASTQEEVKPPFTSEEEK
                     RAGEKGGLFKYVSDVCYVRGDCSLCDIMTVFVAVANIILTSLGGIAVIVFMWGTFGLV
                     MARGNTEAIQANKKVIRGVIVGILIVLLAW"
     gene            complement(582..1952)
                     /locus_tag="VGA49_01345"
     CDS             complement(582..1952)
                     /locus_tag="VGA49_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9664447.1"
                     /translation="MNKKFLATLIILGILVVGLIIFLVIAGGKPETPAQFRPVTLDFW
                     GVFDEPGYFEEIIAAYQVIHPNVNIRYRRLRFEEYERELLEAFAQDRGPDIFAIHNTW
                     IGEYQTLTTPAPAVLTLTFPRIVGTLKKETVVDLRAIPSITLRQLRTNYVDVVYDDVV
                     VNDQIYGLPLSVDNLALFYNRDLLNNAGIPQPPRSWNAFQEQVIKITKVDSRGNILLS
                     GGAIGASRNIERSTDILSVLMMQNGANMVTNDVVTFHRIPPDQPERPRPPGEEALIFY
                     TDFANPSKESYTWNADQPNSVDAFIAGQTAFFFGYAYHIPTIDARAPKLNYSIAKLPQ
                     IEGNRELNYANYWVNVVSRKTADLNEAWDFVQFMSSAEQVSKYLARAKKPTALRGLIN
                     SQLEDLDLSIFASQVLTATSWYRGKDAQAAETILMDMIDTVLAGGLEVNRAINLAAGQ
                     INQTLR"
     gene            complement(1961..2827)
                     /gene="galU"
                     /locus_tag="VGA49_01350"
     CDS             complement(1961..2827)
                     /gene="galU"
                     /locus_tag="VGA49_01350"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX9664448.1"
                     /translation="MAKIRTAIIPVAGSGTRFLPATKALPKEMLPIVDKPIIQFLVEE
                     AVAAGITNIIFVTGRGKRAIEDHFDYSFELTHLLKKKKNHKLLEKIKKIADLASFSYV
                     RQPQPLGDGQAILMARPLVGDEPVAIMFGDDLVDSRVPVIKQLMKVFEKYGDPVIAVE
                     KVPKKEVDRYGVIKGVKIENRTYQVEKIVEKPPVAKAPSNLAVIGKYIITPDVFDILA
                     SLGKDRTGEIRLAGALAELIKHRPVYAYEFEGDRYDCGDKLGFLKATINYGLKHRELK
                     ADFKKYLKSLKI"
BASE COUNT          840 a          712 c          614 g          802 t
ORIGIN      
        1 ccaggccagc aaaactatca agatgccgac aatcacgccc cggatcactt ttttattggc
       61 ctgaatggct tccgtattgc cccgggccat caccaggcca aaggttcccc acataaaaac
      121 aatcacggcg atgccgccca ggctggtcag aataatattg gccacggcta cgaaaacagt
      181 cattatgtcg cacaacgaac aatcgcctct gacataacag acatcgctga catatttaaa
      241 caagccgcct ttttccccgg cccgtttctc ttcctcggaa gtgaatggcg gcttaacttc
      301 ctcctgagtg ctggcagtca aaacaccaaa accaaataat aaatttaagg caaaaagaga
      361 gacaaaagtg attaaaagta ttttctttcc agtcataata attaacgcaa aactaaaatg
      421 tcaaaacgca atctttttct gaaatccaaa tttcaaagct caaatatcaa ttaaagctca
      481 aagtctaaat gtcaaattat ttttatcatt tggattttgg atttgatttg tcatttggat
      541 tttgaaattt gaaattaatg aacgttttga gttttgaatt tttagcgcag tgtctgatta
      601 atctgtcccg ccgccagatt aatcgcccga ttgacttcca ggccgccggc taagacagta
      661 tcaatcatat ccattaaaat tgtttcggcc gcctgggcgt cttttccccg gtaccagctg
      721 gtggcggtta aaacctggga agcaaaaatt gacaaatcca gatcttccag ctggctgttg
      781 attaagccgc gcaaagcagt cggttttttg gctctggcca gatatttaga aacctgctca
      841 gccgaactca taaactgaac aaaatcccag gcttcgttta aatcagcggt ttttcgggag
      901 acaacattaa cccagtagtt ggcgtaattc agctcgcggt tgccctcaat ctggggcagt
      961 ttggcgatac tgtaattaag cttgggcgcc cgggcgtcaa ttgttgggat atggtaagcg
     1021 tagccaaaaa agaaagccgt ctggccggca ataaaagcgt ccacggaatt aggctgatcg
     1081 gcgttccagg tataagattc tttggatgga ttggcaaaat cagtgtaaaa aatcaaagct
     1141 tcctctcccg gcggccgcgg cctttccggc tgatccggcg gaatccggtg gaaggtgaca
     1201 acatcgttgg taaccatatt ggcgccgttc tgcatcatta aaaccgataa aatatcggtg
     1261 cttcgttcta tattccggct ggcgccaatc gccccgccgg acagcaaaat attgccccgg
     1321 gaatcaactt tggtaatttt aatcacctgc tcctgaaaag cgttccagct tcttggcggt
     1381 tggggaatgc cggcgttgtt cagtaaatcc ctgttataaa ataaggccag attatcaaca
     1441 ctcaatggca ggccgtaaat ttgatcattg acaacgacgt cgtcataaac aacatcaaca
     1501 tagttagtcc ttaattggcg taaagtaatt gagggaatag cccgcaaatc aacaactgtt
     1561 tcttttttta aggtgccgac tatccgggga aaggttaaag tcaaaacggc cggggcgggc
     1621 gtggtcaaag tctgatattc gccaatccag gtattatgaa tggcgaaaat atccggcccg
     1681 cgatcctggg caaaagcttc cagcaattcc ctctcgtatt cttcaaatct caaccgacga
     1741 taccgaatat tgacattggg atgaatcacc tgataggcgg cgatgatttc ctcaaaatac
     1801 cccggctcgt caaaaacccc ccaaaaatcc agagtgaccg gccggaattg cgccggggtt
     1861 tccggcttgc cgccggcgat caccagaaaa ataatcaggc caacgaccaa tatccccaaa
     1921 attatcaaag ttgctaaaaa ttttttgttc attttttatt ttatattttt aatgatttta
     1981 aatatttttt aaaatctgct ttcagctccc ggtgctttaa cccataatta atcgttgcct
     2041 ttaaaaaccc cagcttatcg ccgcagtcat agcgatcacc ttcaaactca taagcgtaaa
     2101 ccggccggtg tttaatcagc tcggccaggg cgccggccag cctgatttcg ccggtccggt
     2161 ctttgcccag gctggctaaa atatcaaaaa catccggcgt gataatatat ttgccaataa
     2221 ccgccagatt ggaaggcgct ttggccaccg gcggcttttc aacaattttt tccacctgat
     2281 aagtccgatt ttctattttg acgcccttaa taactccgta acggtcaact tcttttttag
     2341 gaactttttc aacagctatg accggatcgc cgtatttttc aaaaactttc attaattgtt
     2401 tgatgaccgg cacccggctg tccaccaaat catcgccaaa cataatggcc accggctcgt
     2461 cgccgaccag cggccgggcc attaaaatcg cctggccgtc gcccagcggc tggggctggc
     2521 ggacataaga aaaagacgcc aaatcagcga tcttttttat tttttccagc aatttatgat
     2581 tttttttctt tttcaacaaa tgggtcagct caaaagaata atcaaaatga tcctcaatcg
     2641 cccgcttgcc gcggccggtc acgaaaataa tattggtaat gccggcggcc acggcctcct
     2701 ccaccaaaaa ttgaataatc ggcttgtcaa caatcggcaa catttctttg ggcaaagctt
     2761 tggtagccgg caggaatctg gtgcctgatc cggcgaccgg aataatcgcc gtacgtattt
     2821 tagccataat aaatgtgtgt aactttttta aaattataac ataaagctat agacgatgct
     2881 aatatactaa tggatactaa ttatacttat atcccgattc taatatacga attgatacga
     2941 atgaaactaa tgaattagta tattagtg
//
LOCUS       DASWHZ010000056         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2371580, whole genome shotgun sequence.
ACCESSION   DASWHZ010000056 DASWHZ010000000
VERSION     DASWHZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371580"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VGA49_01355"
     CDS             complement(<1..764)
                     /locus_tag="VGA49_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664449.1"
                     /translation="MPKNKTENKKSDAGLKPSPYVLDLKKTIQEREAYKETFEYRSKN
                     FISQLTVSLTAFFDSLRSFKLSKRKQPRRLEEVQKPAYLKNLEMLEDLTAGQDLSLDK
                     LIRQAAVKDDSDSFEQPPVSQINFAKAASVKTQPVKKSIQTPKINIKLPDKHKKVFNF
                     LITSAVIASILIIFVFTTNLVNEKNKVLGATSNALEELKVAKAASYDFDLDLAGAKFD
                     QARKNFIIAQHELDNLNFLAGGLVKILPNKEIKSGR"
     gene            complement(757..1500)
                     /locus_tag="VGA49_01360"
     CDS             complement(757..1500)
                     /locus_tag="VGA49_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9664450.1"
                     /translation="MYFILILVLIVILVSIRQINEYQRGVKFRLGRFVKTINPGWRLI
                     IPLIESMQKIDMRVKAVDVPYQKAITKDNVSVSVNAVIYYKVRDAAKALIEVENFYYA
                     VSQLAQTTMRNVAGEVTLDDLLGERETISNRIKEIVDTATDPWGIQVNSVDLKDITLP
                     EDMERTIAKQAEAEREKRAVIIRAEGEVIAAENMAKAAGILSTKEGALHLRTLQSIND
                     ISSDQSNTIIFAIPLEILRAIEDWRKKNA"
BASE COUNT          423 a          349 c          285 g          494 t
ORIGIN      
        1 atccgtccgg atttaatttc cttatttggc aaaattttaa ccagtccgcc ggctaaaaaa
       61 tttaaattat caagttcatg ctgggcgatt ataaaattct tccgggcctg atcaaatttc
      121 gccccggcca gatcaaggtc aaaatcatag ctggccgctt tggccacttt tagttcctcc
      181 aaagcattgc tggtcgctcc caaaacttta tttttttcat taactaaatt agtcgtaaac
      241 acaaagataa ttaaaataga agcaataacc gctgaagtga tcaaaaaatt aaaaactttt
      301 ttgtgtttgt ccggcagctt aatattaatc tttggggttt ggattgattt tttaaccggt
      361 tgagttttaa cggaagctgc tttggcaaaa ttaatctgac tgaccggcgg ctgttcaaaa
      421 ctatctgaat catctttaac cgccgcctgc ctgattaatt tatccagtga caaatcctgg
      481 cctgccgtta aatcttccag catttccaga tttttcagat aagccggctt ttgaacctcc
      541 tccagccggc gaggttgctt tcttttagac agcttaaatg atcttagact atcaaaaaaa
      601 gcggtcaggc tgacggtcag ctggctgata aaattttttg atctgtattc aaaagtttct
      661 ttgtaagctt ccctttcctg aatagttttc ttcaaatcca aaacatacgg agacggtttt
      721 agtccggcgt ctgatttttt gttttcagtt ttgtttttag gcatttttct tgcgccagtc
      781 ttcaattgct cttaaaattt caagcgggat agcaaaaata atcgtgttgg actgatctga
      841 acttatgtcg ttgattgact gtaaagttct caaatgcaaa gcgccttctt tggtggataa
      901 aatcccggcg gctttagcca tattttcagc ggcaatcact tcaccttcag ctctaattat
      961 aaccgcccgt ttttcccgct cggcttcagc ttgtttggcg atcgttctct ccatgtcttc
     1021 cggcagggta atgtctttca ggtcaacgct gttaacctga atgccccagg gatcagtcgc
     1081 ggtatcaact atttccttga ttcggttgga aatcgtttcc ctctctccca acaaatcgtc
     1141 cagagtaact tcaccggcga catttctcat cgtcgtctgg gccagctggg aaaccgcgta
     1201 ataaaaattt tccacttcaa tcagagcttt ggccgcgtcc cgaactttgt aataaatgac
     1261 ggcgttgact gaaacagaaa cattatcctt ggtgatcgcc ttttgataag gaacatcaac
     1321 cgctttaacc cgcatgtcaa ttttttgcat tgactcaatc aacggaataa tcagccgcca
     1381 gccgggatta attgttttaa caaaccggcc cagccggaat ttaacgcccc gctgatattc
     1441 gttgatctga cgaatactga ctaaaataac aataagaacc agtattaaaa taaaatacat
     1501 aaattggctc ctttctagaa tttaattata caacaaaaaa ccgctaattt a
//
LOCUS       DASWHZ010000057         3403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_512383, whole genome shotgun sequence.
ACCESSION   DASWHZ010000057 DASWHZ010000000
VERSION     DASWHZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512383"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2035)
                     /locus_tag="VGA49_01365"
     CDS             complement(<1..2035)
                     /locus_tag="VGA49_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX9664451.1"
                     /translation="MLASFLQVTLLNEPVALTASLTNNIYFRPLLYLGAGLILILILF
                     SVVLKITRKIYKTPVAFKKIVLLVTLPKESSKEEQRAPTLQEIQEDISVAETLFSAIG
                     GLRAQRGLTAWLFGREDQIALEIVADKNLISFYIALPLYLQRFIEQQLQAQYPESHIE
                     EVEDYNIFSPKGAVSGSYLKFRRSYAFPIKTYRKLDSDPLNAITNAMSKIKDPDGAVV
                     QIVARSSKKVWHGFGRKIASEMQQGKKVNMAIRSARQGIVLRTISEILDLLKTRKKEG
                     EPGKEQYRLSPLEEEMVKGLEEKTSKAGLDVNIRVIASGETDAKAKMNLDNILNAFSQ
                     YNIYEYGNSFARSGPRSQEKIIKDFIYRDYDEKFGIILNTEELASIYHLPLPSTETPN
                     IRWLVAKKAPPPVNVPQEGLVLGYNVYRGEETVIRIKQDDRRRHMYLIGKSGTGKSNF
                     ISYLAAQDIKNGEGVCVVDPHGDLVEDLLYSIPKERIDDVIIFNPSDTDRPLGLNMLE
                     YKNDDQKDFVVQEMISIFYKLFPPEMIGPMFEHNMRNVMLTLMADKEHPGTIADIPRM
                     FTDTAFQNYKLEKVTDPVVRAFWEKEMAQTSDFHKSEMLGYLISKVGRFVENEMMRNI
                     IGQPKSGFDFRDVMDNQKILLVNLAKGKVGDVNSNLLGLIIVSKLQMAAMARAD"
     gene            complement(2095..2619)
                     /locus_tag="VGA49_01370"
     CDS             complement(2095..2619)
                     /locus_tag="VGA49_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664452.1"
                     /translation="MAQQRIDKELNNQSKQTEARPEIRPEVEVGKPETEAKPRPVITE
                     EKPAKEAVEKQAPAVRPKPAEPEPSAPPLAPSRTYAEIERVLDEDLEPIYSQMASREQ
                     KEFKQKEAETATAIEGLLRQIKFKTKNVLELIVKWLKLIPGVNKFFLEQEAKIKTDKI
                     IDIRERERDEKPQK"
     gene            complement(2706..>3403)
                     /locus_tag="VGA49_01375"
     CDS             complement(2706..>3403)
                     /locus_tag="VGA49_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664453.1"
                     /translation="DICSYNDGIAFLNAKLLDLKYHLDTWGYQDYSLDPVGYRKILVP
                     VSKESFLGQMLLADLLSVQTWESLAAIVKYLVTGNRTFQPTVIHFGSLGFRPPLISYY
                     LTRNGAFFNGSFFITRNDQPFLEVQIGASADFIGSQPVKTGFRIGGEFRKFKGYDHWA
                     VPMVSPYLYLNFDRSMNYSGISLGTEIQYDLVEGVVAVRSRLDYSENDLIECEVKGQA
                     NGFGATLDIIISY"
BASE COUNT          861 a          858 c          708 g          976 t
ORIGIN      
        1 tgtcagcccg ggccatggcc gccatttgca gtttggaaac aataattaac cctaacaaat
       61 tgctgttaac atcgccgact ttgcccttgg ccagattaac cagcaggatt ttttgattat
      121 ccatgacatc ccggaaatca aaacctgatt tgggctggcc gataatattg cgcatcatct
      181 cgttttccac gaaccggccg actttggaaa tcaaataacc aagcatctcc gatttatgaa
      241 aatcagacgt ctgggccatt tctttttccc agaaggctct gaccaccgga tcagttactt
      301 tttccagttt ataattttga aaagccgtgt cagtaaacat ccggggaata tcagcgatcg
      361 tcccgggatg ctctttatca gccatcaggg tcagcatgac attccgcatg ttatgctcaa
      421 acatcgggcc gatcatttcc ggcgggaaaa gtttgtaaaa aatagaaatc atttcctgaa
      481 cgacaaaatc tttctgatcg tcatttttgt attccagcat attcaggccc agcggccggt
      541 cggtgtctga cggattgaaa ataatgacat cgtcaattct ttctttggga atggagtaca
      601 gcaaatcttc caccagatcg ccgtgaggat caaccacgca gactccttca ccgtttttaa
      661 tgtcttgggc cgccagataa gaaataaaat tggatttacc ggtgcccgat ttaccaatca
      721 gatacatgtg ccgccggcga tcatcttgtt ttattctgat aacagtctcc tccccccgat
      781 aaacattgta gcccaaaacc agaccttcct ggggaacatt aaccggcggc ggcgcttttt
      841 tggccaccag ccagcggata ttgggcgttt cagttgacgg cagaggcaga tggtaaatac
      901 tggccagctc ctcggtattt aaaataatcc cgaatttctc gtcgtaatcg cgataaataa
      961 aatctttgat aattttttcc tgcgaccgcg ggcccgatct ggcaaaacta ttgccgtatt
     1021 cgtaaatatt atactggcta aaagcgttta aaatgttgtc cagattcatt ttggctttgg
     1081 cgtcagtttc gcccgaagca atcaccctga tattaacatc cagaccggcc ttgctggttt
     1141 tttcttccag gcctttgacc atttcttctt ccagcggcga cagccggtac tgctctttgc
     1201 ccggctcacc ttctttcttc ctggttttca gtaaatccag aatttcgcta atcgttctca
     1261 agactatccc ctgccgggcg gatctgatgg ccatgttaac ttttttgccc tgctgcattt
     1321 ccgaggcgat cttccggcca aaaccgtgcc agactttttt agacgaccgg gcgacgatct
     1381 gaacgaccgc tccgtccgga tctttgatct tgctcatggc gttggtgatg gcgttaagcg
     1441 gatcgctgtc cagcttgcgg taagttttaa tcgggaaagc gtaagaccgc ctgaatttta
     1501 aataactgcc ggagaccgct cctttgggac tgaaaatatt ataatcctcc acttcttcaa
     1561 tatgggactc gggatactgg gcctgaagct gttgttcaat aaatctttgc agatacaaag
     1621 gcagggcgat gtaaaaagaa attaaatttt tatcagccac gatttccaga gcaatctggt
     1681 cttcccggcc aaacagccag gccgtcaacc cccgctgggc ccgcaaaccg ccaatggcgc
     1741 tgaacaaagt ttcagcgact gaaatgtctt cctgaatttc ctgcaatgtc ggcgctcttt
     1801 gttcctcttt ggaagattct ttgggcaaag tcaccagcaa aactattttt ttaaaagcga
     1861 ccggcgtttt ataaattttc cgggtaattt ttaaaacaac agaaaacagg attaatatta
     1921 aaatcaatcc tgctcctaaa tacagcaaag gtctaaaata aatattattc gtcaggctgg
     1981 ccgttaaagc caccggctca ttcagcagag tcacctgtaa aaaactggct aacattttat
     2041 ttttgtttaa atttcttttt gcctgccccg tagcgccccg agcttgtcga ggggctactt
     2101 ctggggtttt tcgtcccgtt ctctttcccg gatatcaata atcttatcag ttttaatttt
     2161 agcttcctgt tccagaaaaa atttattaac acccggtatt aatttcagcc atttaacgat
     2221 cagttccaga acattttttg ttttaaattt aatctgacgc agcaagcctt caatggccgt
     2281 ggcggtttca gcttcctttt gcttaaattc cttttgctcc cgggaagcca tttgcgaata
     2341 aatcggctcc aaatcctcgt ccaaaacccg ttcaatttcc gcgtaggtcc tgcttggggc
     2401 cagcggcggg gcggacggtt cgggttcagc cggttttggc cgaactgccg gcgcctgctt
     2461 ttccacagct tctttagccg gcttttcttc agtaataacc ggccggggtt tagcttcagt
     2521 ctccggtttg ccaacctcaa cttcaggcct gatttcaggc ctggcttcag tctgtttact
     2581 ttggttattt aattctttat caattctttg ctgagccata aatatttact aaattttaac
     2641 atttatccgc taataaggca acaaaaaaac ccggctttct ccaaaaagac gggttggtaa
     2701 caatactaat acgaaataat gatgtccaaa gtcgcgccaa aaccattagc ctgccctttg
     2761 acctcgcatt cgatcaggtc gttttccgag taatcgagcc gggacctaac tgccactact
     2821 ccctcaacca gatcgtactg gatttctgtt cccaggctga tgccgctgta attcattgac
     2881 ctgtcgaaat tcaggtagag atagggacta accatgggta ctgcccagtg atcgtaaccc
     2941 ttgaatttcc tgaattcacc accgatcctg aaacctgtct taaccggctg gctaccgatg
     3001 aagtcggcgc tggcgccgat ctggacttcg aggaaaggct gatcatttct ggtaatgaaa
     3061 aaactgccgt taaagaaagc cccgttcctg gtcagatagt agctgatcag cggcggacga
     3121 aatcccagac tgccaaaatg aatgacagtc ggctggaaag tccggttgcc ggttaccagg
     3181 tacttgacta ttgcggccag gctttcccag gtctgaacgc tcaacaagtc agccagcagc
     3241 atctgcccca ggaaactttc cttactgact ggcactaaaa tttttctata cccaaccgga
     3301 tccagactat aatcttgata accccacgta tcaaggtgat attttagatc aagcaatttg
     3361 gcattcaaaa atgcaatccc gtcattataa ctacagatat cct
//
LOCUS       DASWHZ010000058         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2007771, whole genome shotgun sequence.
ACCESSION   DASWHZ010000058 DASWHZ010000000
VERSION     DASWHZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007771"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VGA49_01380"
     CDS             complement(<1..1500)
                     /locus_tag="VGA49_01380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9664454.1"
                     /translation="MPDKKIQKKEYNISGMHCASCALTIEGNLQKTKGVKSANVNFAT
                     QKATVEFDKRAGDDQKILEIIKKSGYQAAPAAETAGTDRGQILREQEIKKERNLFILS
                     LVLSIPVVTLSMILRDVSLQSKIIQSLLAGIVQFYVGFRFYKGFYYATKNKTANMDTL
                     IAVGTSAAYLYSLATTYLIEGEVFFETSALLITFVVLGKWLEARTKGQAGAAIKKLLG
                     LQAKTARIIQAGREIDVPIKDVRVGDIVIVRPGEKIPVDGQIIDGYSSVDESMISGES
                     LPIEKKQGDFVVGATINKTGSFKFKTTKIGRETVLAQIIKVVEEAQSSKAPIQKFADT
                     VSGYFVPTVVALALITFIIWYFFILAPFVTALLAFTAVLVIACPCALGLATPTAIMVG
                     TGKGAENGLLIKGGEPLETANKIDIIVLDKTGTLTKGQPEVTAIINFIDSKKELLKLA
                     ASVDQKSEHPLAEAIVKKAKMENIKLTDPEKFETIPGHGVVGQISGRLIL"
     gene            complement(1505..1873)
                     /locus_tag="VGA49_01385"
     CDS             complement(1505..1873)
                     /locus_tag="VGA49_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9664455.1"
                     /translation="MQIIGLILIIIVIGLYFFWLYFTKTQKGAMAVEKKGIQTFNILV
                     KGVYSPNIIRTRVNQPVKINFRREESTDCSRYVNFPDFKIRRELPENKTVAIEFTPER
                     KGEFVFTCDMSMYQGKLIVE"
     gene            complement(1875..2561)
                     /locus_tag="VGA49_01390"
     CDS             complement(1875..2561)
                     /locus_tag="VGA49_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEX9664456.1"
                     /translation="MHQYVFLILSLGLLAVWSVIFSMKVTFRKEMLWVSFLTMFLGLT
                     EPFFVPEYWNPPTWLNLAQKTGFDLESFIFTFAIGGLAVSVYEMVFKTKHQKIPVSRR
                     KKRFHLSAIIFAALTFLILYFSTTLNPIYSASIAMLVGFIFTLSCRPDLFLKMLISGS
                     VFLGIYFISLTIINFNFPNYIGSVWNTVNLSGIYLSGLPIEELIWAFSFGLFWSSIYE
                     HLNNYQLKTI"
     gene            complement(2562..2705)
                     /locus_tag="VGA49_01395"
     CDS             complement(2562..2705)
                     /locus_tag="VGA49_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664457.1"
                     /translation="MMWGTNMFGSGMPAWFLSGGFLFWFLWLITSVELVVVLWLLINK
                     LKK"
     gene            complement(2723..2887)
                     /locus_tag="VGA49_01400"
     CDS             complement(2723..2887)
                     /locus_tag="VGA49_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664458.1"
                     /translation="MVRKITQTGENLYQCEACGLFYKEEELAKRCQAWCAEHKSCNLD
                     IIKQAVKKNN"
     gene            complement(2892..>3343)
                     /locus_tag="VGA49_01405"
     CDS             complement(2892..>3343)
                     /locus_tag="VGA49_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664459.1"
                     /translation="YLIYSLTSLHKKLNSRFLTTLETIIIFMLLIGWLGTWGLYYAVS
                     GYDSFVHFFTSFLGTLVVFIVISSFKDLVNKPRIFIILFLLISAYGIFNEFFQHYGDR
                     WWQTTMSGEAGQPYDSYRDVVYNTLGIFLGIAVAYLKGNTIISRLSI"
BASE COUNT          985 a          699 c          703 g          956 t
ORIGIN      
        1 taaaattaga cgtccgctga tttggcccac gaccccgtga ccggggatgg tttcaaattt
       61 ttcaggatca gtcagtttaa tattttccat tttagctttt ttaacgatag cttcagccag
      121 cgggtgttct gatttttgat caactgacgc cgctagtttt aacagttcct tttttgaatc
      181 aatgaaatta ataatcgccg ttacctcggg ctggcctttg gttagggtgc cggttttatc
      241 aagcactata atgtcaattt tattggctgt ttccagcggc tcgccacctt tgattaacag
      301 gccgttttca gcgcctttgc cggtgccgac cataatcgcc gttggggtgg ccaggcctaa
      361 agcgcagggg caggcgataa ccagcaccgc ggtaaaagct aataaggcgg tgacaaacgg
      421 cgccagaatg aaaaaatacc agatgatgaa agtaattaaa gccaaagcca cgactgttgg
      481 cacgaaataa ccggaaacag tgtcggcgaa tttttgaatt ggcgctttgg agctttgggc
      541 ttcttccacc actttaataa tttgagccag gacggtctcc cggcctattt tggttgtttt
      601 aaatttaaaa ctgccggttt tattgatggt ggcgcccact acaaaatcgc cttgcttttt
      661 ttcaatgggc aggctttcgc ctgaaatcat tgattcgtca acgctggagt agccgtcaat
      721 aatctggccg tcaaccggaa ttttttctcc gggccgaaca ataacaatat cgcccacccg
      781 aacatcttta atcggaacgt caatttcccg acccgcctga attatccggg cggttttggc
      841 ctggagaccc agcaattttt taatggccgc gccggcctga ccttttgttc tggcttccag
      901 ccatttgccc aacaccacaa aagtaatcag caaagcggag gtttcaaaaa aaacttcacc
      961 ctctatcaga taggtggttg ccagactgta caaataagcg gcgctggtgc caacggcgat
     1021 taaagtatcc atgttggctg ttttgttttt agtggcgtaa taaaaccctt tataaaatct
     1081 aaagccgacg taaaactgga caatgccggc tagcaaagac tgaattattt tactttgcaa
     1141 agaaacatcc cttaaaatca tgcttagggt aacgaccgga attgataaaa ccagacttaa
     1201 aataaaaagg tttctttctt ttttaatttc ttgttctctt aaaatttggc cgcggtcggt
     1261 cccggccgtt tccgcggccg gagcggcctg gtagccgctt tttttaatta tttctaaaat
     1321 tttctgatca tcgccggccc gcttatcaaa ttcaacagtt gctttttggg tggcaaaatt
     1381 gacattggcg cttttgacgc cttttgtttt ttggagattg ccttcaatgg tcaaagcgca
     1441 ggaagcgcag tgcatgcctg aaatattata ttcttttttc tgaatttttt tatcgggcat
     1501 aaatttattc cacgattaat ttcccctgat acatgctcat gtcgcaggta aagacgaatt
     1561 cgccttttct ttcaggagta aattcaatgg cgacagtttt attttctggt aattctcgtc
     1621 tgattttaaa atcaggaaaa ttaacataac gggagcagtc agttgactct tcccgtcgaa
     1681 aatttatttt aaccggctgg ttaactctgg ttcgaatgat attcggggag taaacgcctt
     1741 tgaccaggat attaaaagtc tggattcctt ttttttcaac agccattgct cccttttggg
     1801 ttttggtgaa ataaagccaa aaaaaataca agccgatgac gattattatt aagatcaggc
     1861 cgatgatttg cattttaaat agttttcagt tgataattat tgagatgttc gtaaatgctt
     1921 gaccagaaca ggccgaaact aaaggcccaa ataagttcct caatcggcag gcctgataaa
     1981 taaatgccag acagattaac cgtgttccag accgaaccaa tatagttggg aaaattgaaa
     2041 ttaataattg tcagggaaat gaaataaatg ccaagaaaaa cagatccgct gatcagcatt
     2101 tttaaaaata aatcaggccg gcaggataaa gtaaaaataa aaccaaccag catggcgatg
     2161 gaggcggaat aaattgggtt taaagttgtt gaaaaatata aaattaaaaa agttaaagcg
     2221 gcaaagatta ttgccgacaa atgaaaacgt tttttccgcc ggctgaccgg gattttctgg
     2281 tgtttagttt taaaaaccat ttcgtagacg ctgacggcca ggccgccgat ggcaaaagta
     2341 aaaataaaag actccagatc aaagcctgtt ttttgggcca gattgagcca ggttggcggg
     2401 ttccagtatt ccggcacgaa aaatggttca gtcagaccca aaaacatcgt caggaagctg
     2461 acccagagca tttcttttct aaaagtgact ttcatggaaa aaatcactga ccagaccgcc
     2521 agcagtccga ggcttaatat gagaaagaca tattgatgca tttatttttt gagcttgttg
     2581 atcaacagcc agaggacgac gaccagctca acgctggtga ttagccacaa aaaccagaaa
     2641 aggaagccgc cgctcaaaaa ccaagccggc atccctgatc caaacatgtt tgttccccac
     2701 atcatatagt tgttcctttc tgttaattat tttttttaac cgcctgttta attatatcaa
     2761 gattacagct tttatgttca gcgcaccagg cctggcacct tttggccagc tcttcttctt
     2821 tatagaaaag tccgcatgct tcgcattgat aaagattttc cccggtttga gttatttttc
     2881 tgaccatagt tttagattga aagccggcta ataattgtat tgcctttaag ataagcaacc
     2941 gctatcccta aaaaaatgcc aagagtatta taaaccacat cccggtagga atcatagggc
     3001 tggccggctt cgcccgacat ggtcgtctgc caccagcgat cgccatagtg ctggaaaaat
     3061 tcattaaaaa ttccataggc gctgatcagt aaaaaaagta ttataaaaat tcgcggttta
     3121 ttgacgagat ctttaaaaga agaaattaca ataaacacaa ccagggttcc cagaaaagaa
     3181 gtaaagaagt ggacaaatga atcataaccg ctgacagcgt aatataaacc ccaggtcccc
     3241 agccagccga ttaaaagcat aaaaataatg attgtttcca gggtagttaa aaagcggctg
     3301 tttaattttt tgtggagact ggtcagggag taaattaaat aag
//
LOCUS       DASWHZ010000059         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3115017, whole genome shotgun sequence.
ACCESSION   DASWHZ010000059 DASWHZ010000000
VERSION     DASWHZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115017"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VGA49_01410"
     CDS             complement(<1..1494)
                     /locus_tag="VGA49_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011186511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX9664460.1"
                     /translation="MIQYKVHKREGNLVDFNKSKIDNAIHKAAQSVNLDDYDYCQKLG
                     QQVVDRVQREYFEQDRPVGVEDIQDIVEEVLIKSDRVSMAKSYILYREKRRLVREHRQ
                     AHYQSKDIMVTKRDGSQRPYNADMINKAIERACVAVKDPIGKVMQIATETELTLYNGI
                     TTEEIDQAIINTAVQNIKDDPDFDTIATRLLLKTIYKGVLGNFYTEDELRQKHQAGFP
                     EYIKKGIEQDLLDKRMPEIYDLEALAAELDLSRDNLFKYIGVATMRKRYMIRDRKHRV
                     METPQYFWMRVAMGLALNEKESNKVALQFYNKMSQLEYISGGSTLIHAGTILPKLSNC
                     FLMEMNDDIDHIGKTVSDVMKLTKATGGIGLSVTKLRCEGSPIRKTNTFSSGPIPFMH
                     IIDSAIRAVSRAGKKLGALCFYMENWHLDFGQFLDLRQNAGDEYRRTRTANTAVLLSD
                     EFMKRVDNDEDWYLFDPLEVSDLNELYGSAFSKRYNEYIEMAEAGKFR"
     gene            complement(1692..>2095)
                     /locus_tag="VGA49_01415"
     CDS             complement(1692..>2095)
                     /locus_tag="VGA49_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9664461.1"
                     /translation="ADPGQAITSLTTEALKNIITGSKLVVVNDYEWELVKQKTGWDES
                     SILDKAGALIITLGQRGSKLLTTTDQFEITAYQADQVIDPTGAGDAYRAGLLYGLKNK
                     FSLKNSAYIGAWLASKAVGARGTQNHTIHKT"
BASE COUNT          590 a          486 c          394 g          625 t
ORIGIN      
        1 ccgaaatttg ccggcttcag ccatttcaat atattcattg tagcgtttgg aaaaagcgga
       61 accgtaaagt tcatttaaat cagacacttc cagcggatca aacagatacc agtcctcgtc
      121 attgtcaacc cgcttcataa attcatcaga caataagacg gcggtgttag ccgtcctggt
      181 ccggcggtat tcatcaccgg cgttctgccg caaatccaaa aactgtccaa aatccagatg
      241 ccagttttcc atgtaaaaac acaaagcgcc caatttttta ccggctctgg aaaccgcccg
      301 gatggccgaa tcaataatgt gcataaacgg aatcgggccg gatgaaaaag tattggtttt
      361 tctgatgggc gaaccttcac agcgcaattt cgtgacactc aagccaattc ccccggtagc
      421 cttggtcagc ttcataacat cagaaacagt tttgccaata tggtcaatat catcattcat
      481 ttccatcaag aaacagttgc taagtttggg caaaattgta ccagcgtgaa ttaaagttga
      541 gcccccggaa atatattcca actgcgacat tttattgtaa aactgcaaag cgaccttatt
      601 agactctttt tcatttaaag ccaaccccat ggctacccgc atccaaaaat attgaggtgt
      661 ttccatcacc cggtgcttcc ggtcgcgaat catatagcgt ttccgcattg tggccacgcc
      721 aatatatttg aataaattat cccgggacaa atccagttca gcggccaaag cctccagatc
      781 ataaatttca ggcattcttt tatccaataa atcctgttca atcccctttt taatgtattc
      841 cggaaaacca gcctgatgtt tttggcgaag ctcatcttca gtgtaaaaat tacctaaaac
      901 ccctttgtaa atggttttta aaagcagtct ggtggcaatg gtgtcaaaat caggatcgtc
      961 tttaatgttt tgaacagccg tgttaataat cgcctggtca atttcttcag tggtaatgcc
     1021 attgtataaa gtcagttcag tttccgtggc aatctgcatt accttgccaa tcgggtcttt
     1081 gacagccacg caggcccgct caatggcttt attgatcatg tcagcgttat acggccgctg
     1141 actgccgtcc cgcttggtga ccataatatc tttgctctga taatgagcct ggcgatgttc
     1201 tctgaccagc cgccgctttt cccggtacaa aatgtaagat ttggccatgc tgactctatc
     1261 agattttatc aggacttctt cgacaatatc ctgaatatcc tccaccccga ccggccgatc
     1321 ctgttcaaaa tattcccttt gaacacgatc aacgacttgc tgaccgagtt tttgacaata
     1381 atcataatca tccaaattaa ctgattgagc ggccttatgg atagcgttgt caattttaga
     1441 cttattgaaa tccactaaat taccttctct tttatggact ttgtactgga tcataatatt
     1501 tgtttgggtt taatgattaa tttttagttt taaaagcgac cacaatatgt tgtgccgcac
     1561 tattaatttt aactactaga tatagtactg tcaaataatc agccatttta aaaatcttgg
     1621 ctaaaataaa agaaaaatcg tcaatgtgtc gctaaatgcc ggcattaacg attaattttc
     1681 ttgtggataa gtcaggtttt atgaattgtg tgattttgcg ttcctctggc tccaacagct
     1741 tttgaagcca gccaggcacc aatataggct gaatttttta aagaaaactt gttttttaaa
     1801 ccataaagga gtccggcccg ataagcatca cccgctccgg tcggatcaat cacctggtca
     1861 gcctgataag ccgtaatctc aaattgatca gttgtagtca gcagttttga accccgctga
     1921 cccaaagtaa ttattaaagc gccggcctta tccaggattg aagattcatc ccagccggtt
     1981 ttttgtttaa cgagctccca ttcataatca ttaacaacga ccaattttga accagtgata
     2041 atgtttttca aagcttcagt agtcaaactg gtgatggcct ggccgggatc agcaa
//
LOCUS       DASWHZ010000060         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_135742, whole genome shotgun sequence.
ACCESSION   DASWHZ010000060 DASWHZ010000000
VERSION     DASWHZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135742"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1637
                     /gene="gatB"
                     /locus_tag="VGA49_01420"
     CDS             111..1637
                     /gene="gatB"
                     /locus_tag="VGA49_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9664462.1"
                     /translation="MQYEPIIGLEIHVQLKTKSKMFCGCDNTGENQPPNTTICPICLG
                     HPGTLPVINKQAVDWSVMAALAINCQIPQISKFDRKNYFYPDLPKGYQISQFDLPIGL
                     EGFLEIEIDGQLKKIGLRRLHLEEDAAKLIHGKNESLVDFNRSGTPLMEIVTKPDIRS
                     PREAKIFLRELRKITRYLEVSEADMEKGHLRCDANISLRPLEVDDEFYPKTEIKNINS
                     FRAVERALEYEFQRQTRLWDEKNPPRQQSTRGWNDDKQITEEQRLKEESADYRYFPEP
                     DLPPLRFEADSIQLISTSLPELPAAKKKRFIEMYGFTADDAQILTDEKELAHFTEQVI
                     SELKEWLSTLEKSEKLDQEKVWPRRQRRGWQKNKKELVKLTAGWLISKLFKLLNENNL
                     SIKQSKVTPENFAELISIIYQGKINSTVAQQVLEEMFAKGSDPSQIIEEKGLEQVSDE
                     SQLEKIVNHVIANNPDELAKYKAGKTALFQFFVGQVMKETKGKANPEVVAKLLKKKLI
                     "
BASE COUNT          532 a          374 c          363 g          389 t
ORIGIN      
        1 atctggaaaa taatcaaagc aatgctttga gccaatagcc aacagtcagc agccaacagg
       61 gagtaaaaag tctgttgact gttcaattgt tgactgttga ctaaaaaaat atgcaatacg
      121 aaccaatcat tggtctggaa atccacgtcc agttaaaaac caaatcaaaa atgttctgcg
      181 gctgcgacaa caccggtgaa aaccagccgc ccaacacgac catttgtccg atttgtctgg
      241 gccatccggg aactttaccg gtcattaata aacaggcggt tgactggtcg gtcatggccg
      301 ctttggccat taactgtcag attccccaaa tctccaagtt tgaccgaaaa aattattttt
      361 atcccgacct gcccaaaggc tatcaaatat cacaatttga tctgccaatc ggtttggaag
      421 gttttctgga aattgagatt gatggtcagc ttaaaaaaat cggcctgcgc cgcctccatc
      481 tggaagaaga cgccgcgaaa ctaatccatg gtaaaaacga atctttggtt gattttaacc
      541 gatccggcac tcctctgatg gaaattgtta ctaagcccga tatccgctct ccccgggaag
      601 ccaaaatatt tctgcgggag ctaagaaaaa taacgcgcta cctggaagtc tcggaagctg
      661 acatggaaaa gggccatctg cgctgcgacg ccaacatttc tctccggccg ctggaagtag
      721 acgatgaatt ttatcccaag actgaaatta aaaacatcaa ttctttccgg gcggtggagc
      781 gggccctgga atatgaattt caaaggcaaa ccaggctctg ggatgaaaaa aatcctcccc
      841 ggcagcagtc aacccgcggc tggaatgacg ataaacaaat tactgaagaa cagcgtctca
      901 aggaagaatc agccgactac cgctattttc cggaaccaga cctgccgcct ttgagatttg
      961 aggcggattc aattcaattg atttccacca gtctgcctga attaccggcc gccaaaaaga
     1021 aaaggtttat tgaaatgtac gggtttaccg cggatgacgc ccagatattg actgatgaaa
     1081 aagaactggc tcattttact gaacaagtca tttccgaact aaaagaatgg ctgtccactc
     1141 tggaaaaatc tgaaaaacta gaccaggaaa aagtctggcc ccgccgacaa agacggggct
     1201 ggcaaaaaaa caaaaaagaa ctggtcaaat taacggccgg ctggctgatt tccaaactgt
     1261 ttaaactgct taatgaaaat aatctgtcaa ttaaacaatc aaaagttacc ccggaaaatt
     1321 ttgctgaatt gatttcaatc atctatcagg gcaaaattaa cagtactgtc gcccagcagg
     1381 ttttggaaga aatgtttgcc aaaggctctg atccatcgca aattattgag gaaaaaggac
     1441 tggagcaggt cagcgacgaa agccagctgg aaaaaattgt taatcatgtc attgctaata
     1501 atccggatga gctggccaaa tataaagccg gcaaaaccgc cctttttcag ttctttgtcg
     1561 gccaggtgat gaaggaaact aaggggaagg ctaatcctga agtcgtagct aagttactca
     1621 aaaagaagtt aatctagatt caagatttca gcctccgg
//
LOCUS       DASWHZ010000061         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_522082, whole genome shotgun sequence.
ACCESSION   DASWHZ010000061 DASWHZ010000000
VERSION     DASWHZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522082"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1152
                     /locus_tag="VGA49_01425"
     CDS             223..1152
                     /locus_tag="VGA49_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664463.1"
                     /translation="MEIEGFITPIASLLTKLTELEEEVVSEKEILGTLAKKGFSPDGG
                     TTNKEVFHPGLMCQTSGRARAVRKEVIRQVRQKPEILKEEREAWWSRLKKIFDNRAEI
                     TAGPEASGLAKEIKELLDQRFKVNQKKFNRIDKKIKAGLSYRQFVAILDLLALENSPH
                     WQEKEGQIVCRLTPLEIICQMRLINHLVKTKVKFLSDIETLANKFEAEREKHLYQEKH
                     LRMFLAITACDILKKNCPTGLSVGHRYLLEEKEDGRAKLRFEVFNSQTGEYEYLAGVH
                     QKSEPFIDRFDRFLKEIESRLVFPNLETANKGG"
     gene            1204..1509
                     /locus_tag="VGA49_01430"
     CDS             1204..1509
                     /locus_tag="VGA49_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664464.1"
                     /translation="MALKFIGLKVKGKTWLRGGLFLVSWPVRLFKGLGGQIISRQSEI
                     NCPHCDQPVDLIRAGACLGCGFREEKHIWAGCGNCGQTSEFFNCPDCGLSVRRPWWS"
     gene            complement(1625..2020)
                     /locus_tag="VGA49_01435"
     CDS             complement(1625..2020)
                     /locus_tag="VGA49_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9664465.1"
                     /translation="MKNKPTKLIYPELSYLITGLCFEVHNELGRFAREKQYGDLLELK
                     LKEGKINYAREYNIAKTGNRIDFIIDNKIIAETKTATFITKQDYYQIQRYLQILDKKL
                     GLIINFRSKYLKPLRIVKIDTDVRYKFIK"
BASE COUNT          592 a          385 c          515 g          627 t
ORIGIN      
        1 tggatagcat aattctctct tcttgggtaa aatgttgata agacgccata ggtgttaggt
       61 taatagttag tacaatattt ctattatacc taaccctggg cgtcttttgg tgttttatgc
      121 gcttgcggtt agaatacacc gactccgggt tgacaaattg attcttttcc tttactataa
      181 aaagttctag ttcttttaac attaactggt caggaggtga ccatggaaat agagggattt
      241 ataaccccaa ttgccagcct cctcacaaag ctgacagagc tggaagaaga ggttgtctcc
      301 gaaaaagaaa ttctggggac attggctaaa aagggatttt ccccggatgg cggaacgacc
      361 aataaggagg ttttccaccc cggtttgatg tgtcaaacca gcggccgggc cagagctgtg
      421 cggaaggagg tgatccggca ggttagacaa aaacccgaga ttttgaaaga agaaagggag
      481 gcctggtgga gccgactgaa gaagattttt gacaatcggg ccgagattac tgccggacca
      541 gaagccagcg ggctggccaa agaaattaaa gagttgttgg atcagaggtt taaggtcaat
      601 cagaagaagt ttaacaggat tgacaaaaag atcaaagctg gcttgagtta tcggcagttc
      661 gtggccattc ttgacctctt ggctttggaa aacagtccgc attggcagga aaaagaaggt
      721 caaattgtct gtcggctgac ccccctggaa ataatttgcc agatgcgcct gatcaaccat
      781 ttggtcaaga caaaagtgaa attcttgtcg gatattgaga cgctggcgaa caagtttgag
      841 gccgaaaggg aaaaacattt gtatcaggaa aaacatttga ggatgtttct ggcaataacc
      901 gcctgtgata ttctgaaaaa aaattgtccg acaggtctgt ccgttggcca ccgttatctg
      961 ctggaagaaa aagaagacgg gcgcgccaaa cttcgctttg aggttttcaa tagtcagaca
     1021 ggtgaatacg aatacctggc tggcgtccac caaaagtcag aaccatttat tgatcgtttt
     1081 gatcggtttt tgaaggagat agaaagccgg ttggtttttc ccaatctgga gacagctaac
     1141 aaaggagggt aggttttgag ccagcgactg attggactga ttctggccat tggactgttg
     1201 cttatggcgc tgaaattcat tggccttaaa gtcaagggca aaacctggct aaggggcggc
     1261 ttgtttcttg tcagctggcc tgtcaggctg ttcaaaggcc tgggtgggca aattatcagt
     1321 cgccagtcag aaatcaactg tccccattgc gatcagcccg ttgatttgat ccgggccggt
     1381 gcctgcctgg gctgcggctt tcgggaggaa aaacatattt gggccgggtg cggcaattgc
     1441 ggacagacca gcgagttttt taactgtccg gactgcggtt tgtcagtcag gcgaccgtgg
     1501 tggagctaac agcggaaaat caatttaggt tttctgcttt tttatgctac taataccgat
     1561 gctaatatac ttatggatac taatgaaact aattttgtaa tactaatata ctaatgcata
     1621 cgaattattt tatgaattta tagcggacat cggtgtcaat ttttacgatt cttagcggtt
     1681 taaggtattt acttctgaag tttatgataa gaccgagttt tttgtctaaa atttgtagat
     1741 agcgttgtat ttgataataa tcttgttttg ttataaatgt cgcggttttt gtttccgcga
     1801 taattttatt atcaattata aaatcaattc gattgcctgt tttggcaatg ttgtattctc
     1861 tcgcatagtt tattttgcct tcttttaatt tcagttcaag caggtcgcca tattgttttt
     1921 ctctggcaaa ccggcctagt tcattgtgaa cttcaaagca gagcccggtg ataagataag
     1981 acaattctgg ataaataagt tttgtcggtt tattcttcat atttttaaat tagccaataa
     2041 aaaagcccct caaaataggg ggtggaaatt taatttgatt atactttaaa aattacataa
     2101 gtcaaggata gtttaaccg
//
LOCUS       DASWHZ010000062         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2011372, whole genome shotgun sequence.
ACCESSION   DASWHZ010000062 DASWHZ010000000
VERSION     DASWHZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011372"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGA49_01440"
     CDS             <1..282
                     /locus_tag="VGA49_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664466.1"
                     /translation="FDRAVDYKIYRKNLLTAWPVWPRWLFLFYYVYDLVRREKIEYLQ
                     VGQVLPVGAVALIYKKNFKPALPCLHSRNGFVVSPKDWPEEIFIKENSG"
     gene            224..991
                     /locus_tag="VGA49_01445"
     CDS             224..991
                     /locus_tag="VGA49_01445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9664467.1"
                     /translation="MLSAQRTGRKKYLLKKILDNADRIVTVSQFNVEQLKKININPER
                     IFLIPPGLTPPEAVTEEKLNDLRQRFQLADKKKLLTIGRLVARKGHDQVVRALPAVLK
                     KIPNLVYIIAGDGDYRQDLERLVSQNNLENQVIFTGRVSETDKSALYDLADVFIMPSR
                     QIDDFDVEGFGIVFLEAGHYGKPVIAGQSGGVADAVVDGQTGLLVDPENINEISQAII
                     KLLTDPELADKLGRQGQQRVIAEFNWKNQAAKLCTIL"
     gene            976..>1804
                     /locus_tag="VGA49_01450"
     CDS             976..>1804
                     /locus_tag="VGA49_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664468.1"
                     /translation="MYNIIMFNMSPASDWQNGIVNRNFFVLGELAQRPEINKIMSVDL
                     LPMRGPAPFGLKRVIKYIIESLLPKTDLEIIQKNLTSRCFKKDNIYYYSAVDLYLNKK
                     YFFKNFKNLLGQLDFYKSDCRTIVWSYNPLWLDYYDELESDLKIFDTVDNWSVHASYQ
                     NYYDQLRENYKIIDQKADLILTVAEELRELYQNKSKVHWIPNGVEIAHFRNSPAKTDN
                     QQKIIGYIGTIQNRFDVDLAKYLAKNNPDKKFVIGGPVWMESLSEIENKLKPLNNVYL
                     "
BASE COUNT          570 a          312 c          398 g          524 t
ORIGIN      
        1 ttcgaccggg cagttgatta taaaatttac cgaaaaaatt tactgacagc ctggcccgtc
       61 tggcccagat ggcttttttt gttttattat gtttacgatt tagtcaggcg ggaaaaaatt
      121 gaatatttgc aggttggaca agttttgcca gtcggcgcgg tcgctttaat ttataaaaaa
      181 aattttaaac ctgccttacc ttgtttacat tcacggaatg gatttgttgt cagcccaaag
      241 gactggccgg aagaaatatt tattaaagaa aattctggat aatgctgatc ggatagtaac
      301 agtcagccag tttaatgttg aacagttaaa gaaaataaat atcaatccgg aaagaatttt
      361 cctgatcccg cccgggttaa caccgcctga agcggtcact gaagaaaagt taaatgatct
      421 tagacaaaga tttcagctgg cagataaaaa aaaattattg acaatcggcc ggctggttgc
      481 cagaaagggg catgatcagg ttgtccgggc tttgccggct gttttaaaga aaattccaaa
      541 cttagtttat attattgccg gcgatggcga ttatcgacag gatttggagc ggctggtcag
      601 ccagaataat ctggaaaatc aggttatttt tacggggcgg gtgtcagaaa ctgataaatc
      661 agccctgtat gatttagctg atgtttttat aatgccgtcc cggcaaattg acgactttga
      721 tgtggaaggg tttgggattg tttttttgga agccggccat tatggcaagc cggttatcgc
      781 cggccagagc ggcggagtgg ctgacgccgt ggttgacggc cagactggtt tgctggttga
      841 tcctgaaaat ataaatgaaa taagtcaggc aattattaaa cttttaactg atccagagct
      901 ggccgataaa ctgggccggc agggacagca aagagttatc gctgaattta actggaaaaa
      961 tcaagccgcc aaactatgta caatattata atgttcaaca tgagtccggc ttcagactgg
     1021 caaaacggga ttgtcaaccg taattttttt gttttaggtg aactggctca acgacctgaa
     1081 ataaataaaa taatgtcggt tgatcttttg ccaatgcggg gaccagcgcc ttttggcctg
     1141 aaaagagtca tcaaatatat aattgaatct ttactaccca aaacagattt ggaaattatc
     1201 caaaaaaatc tgaccagccg ttgttttaaa aaagacaata tttattatta ttcagcggtt
     1261 gatctttatt taaataaaaa atattttttc aaaaatttta aaaatctgct ggggcagctt
     1321 gatttttaca agtcagattg ccggactata gtctggtctt ataatccact ttggcttgat
     1381 tattatgacg aactggaaag cgatttaaaa atttttgaca ctgttgataa ctggtcagtc
     1441 cacgccagtt atcaaaatta ttatgaccag ctgagggaaa actataaaat tattgaccaa
     1501 aaagccgact tgattttaac agtggccgaa gaactgcggg agctgtatca gaataagtca
     1561 aaagttcact ggattccaaa cggggttgaa atcgcccatt ttaggaatag cccggccaaa
     1621 actgataatc agcaaaaaat tatcggttac atcggcacga ttcaaaacag atttgatgtt
     1681 gatctggcca aatatttggc taagaataac ccggataaaa agttcgtgat tggcgggcct
     1741 gtctggatgg aaagtttatc tgaaattgaa aacaaactta aaccattaaa taatgtttat
     1801 ttaa
//
LOCUS       DASWHZ010000063         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_138525, whole genome shotgun sequence.
ACCESSION   DASWHZ010000063 DASWHZ010000000
VERSION     DASWHZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138525"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1905
                     /gene="pheT"
                     /locus_tag="VGA49_01455"
     CDS             60..>1905
                     /gene="pheT"
                     /locus_tag="VGA49_01455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9664469.1"
                     /translation="MFISLNWLKDFVDLSKISPRDLGLKLTIKTMEIENVVEQGQNLE
                     NIVIGQVLEIKDHPNADKLKIVVVDDGSGQREVVCGGLNLKQGQLVALARQGARVSWH
                     GQEDLTELKKTKIRGVESSGMICAAEEIGFEKEGKAGEIMDLSYLGKVRLGTPLKKAL
                     ELDDTLFEIDNKSITHRPDLWGHYGVAREVAAFLGKKLKKYPAPDFKAGSAADLKIKV
                     ADFKHCRRFMGVMVGNIRIEPSPLWLVNRLRAVNIRPINNIVDITNYVMTELGQPMHA
                     YDRQMIRGDQLGTRLAKAGEKFTTLDEESWTLTKDDLLIVDQKGPVGLAGLMGGLKSA
                     ITDQTKEIILEAANFDPVTVRKTSKRLGLRSDSSARFEKSLDPDWPELAIKRAVSLIK
                     QLIPEAEVISPVVDQTPVARGRRGKLNQGPIVLNKSYLAMKMGLELADREVIKILKDL
                     GFEVNSTKADYKVIAPTWRATKDIAIPDDLVEEVARIYGYDQIPTKLPNIDITPPARN
                     LELLVERKIKNFLVYGPGWTEVYNYSFVNEKQLENLGIDYSRHIKIQKPLSSDQTLLR
                     QSLIPSLLKNTVDNLRFYDQFKLFEIEQVFLPDLKGPVKDPSGKDKLPG"
BASE COUNT          561 a          372 c          472 g          500 t
ORIGIN      
        1 ttttgtaatt tgaaatttaa tagcttattc tcattccctc tgattccttt ttagataata
       61 tgtttatctc ccttaactgg ctcaaagact tcgtcgattt gtcaaagatt tcccccaggg
      121 atcttggttt gaaattgacc ataaagacaa tggagattga aaatgtcgtt gagcaggggc
      181 aaaaccttga aaatattgtt attggccaag ttttggaaat taaagatcat cctaacgctg
      241 ataaactgaa aattgtcgtg gttgacgacg gctctggcca aagagaggtt gtttgcggcg
      301 ggttgaattt aaaacaaggc cagctggtgg ctttggccag gcagggagcc agagtcagct
      361 ggcatggcca ggaagattta actgaactga aaaaaactaa aattaggggc gttgagagca
      421 gcggcatgat ttgcgccgct gaagaaattg gttttgaaaa agaggggaag gccggtgaaa
      481 tcatggacct gtcttatttg ggtaaagtca ggctggggac gcctttgaaa aaagctttgg
      541 aactggacga tactttattt gaaattgaca ataaatcaat tacccaccgg ccggatcttt
      601 ggggtcacta tggcgtcgcc cgggaagtgg ccgcttttct cggtaaaaaa ttaaaaaaat
      661 atccggcgcc agattttaaa gccggttcag ccgctgattt aaaaatcaaa gtggctgatt
      721 ttaaacattg ccggcggttt atgggagtga tggttggcaa tatcagaatt gaaccgtcgc
      781 cgctctggct ggtcaatcgt ttgcgggcgg ttaatatccg gccaatcaat aatatcgtgg
      841 atattaccaa ttatgtgatg actgaactgg gccagccgat gcacgcttac gaccggcaaa
      901 tgatcagggg tgatcagctg ggcacccggc tggccaaagc tggtgaaaaa ttcactactc
      961 tggatgaaga aagctggact ttgaccaaag acgatttatt aattgttgat caaaaagggc
     1021 cggttggtct ggccggcctt atgggcgggt tgaagagcgc cattaccgat cagactaaag
     1081 aaataatttt agaagcggct aattttgatc cggtaaccgt cagaaagact tccaaacggc
     1141 tggggttaag aagcgattcg tcagcccgtt ttgaaaaaag tctggatcct gactggcctg
     1201 aactggccat taagagggcc gtaagtttaa ttaaacaact gatcccggaa gccgaagtaa
     1261 tcagtccggt tgttgaccaa acccctgtcg cccgcgggcg acggggtaaa ctgaatcagg
     1321 gcccaattgt tttaaacaag agttatctag ccatgaaaat gggtcttgaa ctggctgaca
     1381 gggaagttat taaaatatta aaagatttgg gttttgaagt taattcaacc aaagctgatt
     1441 ataaagtcat agcgccgacc tggcgggcca ccaaagatat agcgattcct gatgatctgg
     1501 tggaagaagt cgccagaatc tacggctatg atcaaattcc aaccaaactg cccaacattg
     1561 acatcacccc accggccaga aatctggaat tattggttga gcgaaaaatt aaaaattttt
     1621 tggtgtatgg tccgggttgg actgaagtct ataattattc ttttgttaat gaaaagcaac
     1681 tggaaaatct gggaattgat tattccagac atattaaaat ccaaaaaccg ctgtcatcag
     1741 accagactct cctgcgccag agcctgattc ccagtttact gaaaaacact gttgataatt
     1801 tgagattcta cgatcagttt aaactgtttg aaattgagca ggtgtttctg ccagatttaa
     1861 aagggccggt caaggacccg tccggcaaag acaaactgcc gggcc
//
LOCUS       DASWHZ010000064         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1641974, whole genome shotgun sequence.
ACCESSION   DASWHZ010000064 DASWHZ010000000
VERSION     DASWHZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641974"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1797
                     /gene="lepA"
                     /locus_tag="VGA49_01460"
     CDS             <1..1797
                     /gene="lepA"
                     /locus_tag="VGA49_01460"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX9664470.1"
                     /translation="RNFAIIAHIDHGKSTLADRLLETTGTVDKREMKEQLLDQMELER
                     ERGITIKLQPVRMDYQVRDGQKYILNLIDTPGHVDFSYEVSRSLAAVEGALLLVDATQ
                     GVQAQTLANLYLALDQNLKIIPVVNKIDLPAANPQKVAEEISNLIDCQPEDILLVSAK
                     TGQGVPEILEKIVELTPPPAETAHESFRGLIFDSNFDEFKGVIAYIRVVDGAVKKGDQ
                     IKFMATQAVITVLDVGYFKPHLKSAEVLKSGEIGYVVTGLKEVGQCRVGDTIAAENVK
                     TRLPGYKEMKPMVFAGIYCKEGDDFSRLREAIEKLKLSDASLVYEPENSPALGFGYRA
                     GFLGLLHLEIFQERLRREYDLDLIITAPSVAYKVILKNNQEIIIKSPQELPDQTQIER
                     IDEPIMRLDVFTPKDYIGGIMTLVQEKRGVYLTTEYLDDNRAILHYEVPLAAVLVDFY
                     DKLKSISSGYASLNYEFKEYQPAAIIKLEILVAEDKIDALSTLVYEDSAYQTGRKIVD
                     ELKNVLTRQMFEVKIQAAVGGGDKSGPGKIIAATRLPPMRKDVTAKLYGGDVTRKRKL
                     LEKQKKGKKKMKLMGLGKVDIPPEAFIAVLKR"
     gene            2049..>2522
                     /locus_tag="VGA49_01465"
     CDS             2049..>2522
                     /locus_tag="VGA49_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX9664471.1"
                     /translation="MRDKRWVLKEQVTEEFKNKFPEIDEIVLQLLYDRQLDSQEKIDE
                     FLNPDYSQDIHDPFLFQDMPAAVERVYQAIEKKEKITVHGDYDADGVSGSAILVSSLK
                     RLGADVDVYIPHREKDGYGINAKTVEFLHQQKTDLIITTDCGISNKAEIDKAQDLG"
BASE COUNT          768 a          481 c          611 g          662 t
ORIGIN      
        1 cgcaattttg ccatcatcgc ccacattgac catggcaaat caaccctggc tgaccggttg
       61 ctggaaacaa ccgggaccgt tgataaaaga gaaatgaaag aacagctgct ggatcagatg
      121 gagctggaaa gggaacgggg gatcactatc aaactccagc cggtcagaat ggactatcag
      181 gtccgggacg ggcaaaaata tattttaaat ttaattgaca ctcccggcca tgttgatttc
      241 agttacgagg tttcccggtc actggccgcc gtggaagggg ctttactgct ggttgacgcc
      301 acccagggcg ttcaggccca gacactggct aatttatatc tggctttgga tcaaaattta
      361 aagattattc cggttgtcaa caaaattgac ctgccggccg ccaatcctca aaaagttgcc
      421 gaagaaattt ctaatttaat tgactgtcag ccggaggata ttttgctggt ttcggccaaa
      481 accggccagg gcgtgcctga aattttggaa aaaattgttg agcttacgcc acctccggca
      541 gaaacagctc atgaatcatt tcggggatta atttttgatt ctaattttga tgagtttaag
      601 ggagtaattg cttatatacg agtcgtggac ggcgcggtta aaaaaggcga ccagattaaa
      661 tttatggcca cccaggctgt aattactgtt ctggatgtcg gttattttaa acctcatttg
      721 aaatcagccg aagttttgaa atcaggcgag ataggctatg tggtgaccgg cttaaaggaa
      781 gttggtcagt gccgggtggg tgacacaatt gccgcggaaa atgttaaaac cagactgccc
      841 ggttataagg aaatgaagcc aatggttttt gccggaattt actgcaaaga aggcgatgat
      901 ttctccagac taagggaagc cattgaaaaa ttgaaattaa gcgatgcttc cttagtctat
      961 gagccggaaa actcgccggc tctgggattt ggctaccggg ccggatttct cggtctgctg
     1021 cacctggaaa tttttcagga aagattacgc cgggaatatg atcttgattt gatcattacg
     1081 gcgccgtcgg tcgcttacaa agtaatttta aagaataacc aggagataat tataaaaagt
     1141 ccccaggaac tgcctgatca aacccagata gaaaggatag atgaaccaat tatgagactg
     1201 gatgttttca cgcccaaaga ttatattggc ggcattatga ctctggttca ggaaaaaagg
     1261 ggcgtttatc taaccacgga atatcttgat gacaaccggg cgatcttgca ttatgaagtc
     1321 ccgctggcgg ccgtcctggt tgatttctac gataaactca aaagcatttc ttccggttac
     1381 gcttcgctaa attatgaatt taaggaatac caaccggcgg ctatcattaa acttgaaatt
     1441 ttagtggccg aagacaaaat tgacgccctg tcaactctgg tttatgaaga ttccgcttac
     1501 cagaccggcc gaaaaatcgt tgatgaactg aaaaatgttt tgacccgtca gatgtttgaa
     1561 gttaagattc aggcggctgt tggcggcggc gacaaaagcg gtccgggtaa aattatcgcc
     1621 gccaccagat tgccgccaat gagaaaagat gttacggcca agctttatgg cggcgatgtt
     1681 acccgaaaga gaaagcttct ggaaaaacaa aaaaaaggca aaaagaaaat gaaactgatg
     1741 ggcctgggca aggttgacat cccgcctgaa gcattcattg ccgttttaaa gcgttaaaaa
     1801 atttgttata atgtaaatct atgaaaaaaa ggactgttaa taaaattatc tggattattt
     1861 tggcggtact gatgattgct tctatgctga tatttacgat tgggccgcta gcttactaat
     1921 taggcaatag gcacaaggca caaggcaata gggtaggaat atttattaga tggggattag
     1981 gcaaataaga gtaggcatga ggcacaaggt aatggggatc aaaattattt ttttataaaa
     2041 attagattat gcgtgataag aggtgggttt tgaaggaaca agttactgaa gaatttaaaa
     2101 ataaatttcc tgaaatagat gaaatagttc ttcagctgct ctatgatcgt caactggact
     2161 cccaggagaa aattgacgag tttttaaatc cggactactc ccaggatatt cacgaccctt
     2221 ttttatttca agacatgcca gcggccgttg aacgagttta tcaggctatt gaaaaaaagg
     2281 aaaaaataac ggttcacggc gattatgacg ctgacggcgt cagcgggtca gctattttag
     2341 tcagttcttt gaaaagatta ggagctgatg ttgatgttta tattccgcac cgggaaaaag
     2401 atggttatgg cattaacgcc aagacagttg aatttctcca tcagcaaaaa acagatttga
     2461 ttattaccac tgactgcggc atcagcaata aggccgagat tgacaaagcc caggatctgg
     2521 ga
//
LOCUS       DASWHZ010000065         7741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2015251, whole genome shotgun sequence.
ACCESSION   DASWHZ010000065 DASWHZ010000000
VERSION     DASWHZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015251"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..149
                     /locus_tag="VGA49_01470"
     tRNA            76..149
                     /locus_tag="VGA49_01470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Lys,seq:ttt)
     gene            346..3039
                     /locus_tag="VGA49_01475"
     CDS             346..3039
                     /locus_tag="VGA49_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX9664472.1"
                     /translation="MIQKELWYNLSVSEILKKFKTSQAGLSQKEAVGRLKKFGPNELP
                     REKRLTWFKIFLSQFKSPLVYILLLAAVITLILQEFIDMAVIIAAVFINVIVGFFQEY
                     KADQALWQLKKLTKIEAKVIRQGILHQIDSAEIAPGDIIIFEAGDKIPADGRLIEVNN
                     LEIAEAALTGESVPSTKIDNVLKRGTALADRENMVYMGTTVERGKGRAVVTATGVQTE
                     IGRIAVIVREIPEEKSPLQKKLKKLTARLTLILGVISAGIFTFGFLTGQPFFEMLITT
                     VALAVAAIPQGLPASVSIILAIGMQKILKRKGLVRQMVAAETLGATTVICTDKTGTLT
                     KGVMRVSQVLTGSRELLQGKHKVFTLPGRAEEIGHILALEISLLCNNSFIENPEDQLK
                     DWIIRGDSTETALLLAAVQAGLNRSQLEKESPRLAEVPFDSEKKYMVTMNKLDSQHNI
                     LYLKGAPEVILEMSSAINVDGKESKLTPNKIKILKKTYEDLTRKGLRVLGVGYRKVKI
                     QNSKALEELCCDFIFVGFIGLKDPLRKEAKETLRKIYQAGIRPVVVTGDHKLTARAIF
                     EELGFPVKSDRIMEGVELDQLSDAELKQKITEIDVFARVEPKHKIRIVDAWQARGQVV
                     AMTGDGVNDAPALKSADIGVALGSGTDVAKETADIVVTDDNLSTLVAAVEQGRIIFEN
                     IRKVTLYFLIDSFTEIILIGGSLLLGLPLPLLAAQILWVNLVEDSFPSIALAFEPGER
                     EVMKDRPRKLTEPILDTEIKVLIFVIGIITDLFLFLLFIWLLGRDYSMEYIRTIIFIT
                     LGLDSLFYVFSCRSFRHTIFTRSIFTNKLLIAAVLTGMMFILIGVYMPIMRTVLRTVP
                     LSFSDWLIPVGLSVLGVILIEITKEIFIIRGRRKKFSEAVY"
     gene            3050..3691
                     /locus_tag="VGA49_01480"
     CDS             3050..3691
                     /locus_tag="VGA49_01480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9664473.1"
                     /translation="MKIVKINPVRPSPKIIGQVVKILQGGGVVMFPTETVYGLGADFK
                     NQAGVKKIFQIKGRHLKKALPVIVSGLGQIKSIAEVDKLAYNVINKFLPGPLTIILPK
                     KKVVSGLISAGTKTVAVRYPDHSVALKLSSRFPLASSSANLSGQPACYSVSQFLKQVK
                     GQQNLPDLILDAGRIPKVKPSTIVDLTGKKIKILRKGPVSNFHNKYVQKKISR"
     gene            3666..5249
                     /gene="gpmI"
                     /locus_tag="VGA49_01485"
     CDS             3666..5249
                     /gene="gpmI"
                     /locus_tag="VGA49_01485"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9664474.1"
                     /translation="MSKKKLVVEIVIDGWGIDPSEKGNAFLMAKTPVFDDLISHYPFA
                     AIEASGLDVGLPWGEVGNSEVGHTNLGAGQVIYQNLPRINIAIEDKSFFSNEVFLKAV
                     QHARKNNSDLHLTGLLSDGGVHGQTDHLFALLKLIKERKFKRVFIHAITDGRDTAADL
                     APKYLKKLDDQLKVNGLGRLATIIGRYYAMDRDEKWEKTQKACDLLTQGLGEKTREPI
                     KTIEKFYKKKIDDEMMEPIVIVDHHDQPVGLIKENDSVIFFNFRPDRARQLTKAFVLD
                     NFKGFKRDKLKNLFFATMMEYVEGLPVKVAFPPQLITEPLGKIIADHGLKQLRIAETE
                     KYAHVTYFFNGGSEIQYKNEDRKIIPSPTVKSFDQKPEMSAFKLTDEVIARINSKKYS
                     FILMNFANFDMVGHTGQIKAAIRAIEVIDECLSRIVPAVLQQNGQVVITADHGNVEVM
                     IDPVTGKVNKSHTTSPVPLLIIDESKKVDRTPDEVEMFKRDIATIGILSDVTPTVLEI
                     MGLKQSKEMTGRSLLGDMT"
     gene            5435..6751
                     /locus_tag="VGA49_01490"
     CDS             5435..6751
                     /locus_tag="VGA49_01490"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX9664475.1"
                     /translation="MINTLRKITPRFIKNFYHKTLALLASFIYGFPSRKIKIIAITGT
                     DGKTTTCYLTTSILETAGYKVAELSGIRFKIGDKEWPNKTDNTTPGRFKLRKFISRAV
                     KNKCDYLVLEVTSWALAQYRVKNVSIDIALLTNLTLEHLDLHKTMAEYRQAKGRLFEM
                     LSTGYYLENVPKISIVNLDDNDAQYFLSFKADQKITYGIETATDISAENVKPGADKID
                     FDLLLNEQSVRVSFKLPGLYNVYNALAAAAVGSALKINSEKIKQGIEKIEYVPGRMER
                     IDAGQDFTVVVDFAHTPNGLKELFKAARTMIEPGKKIIAVYGSAGGRDKEKRPLIGEV
                     AGQLVDFSFLTTDDPRNEDPLKIAQTIEIGLKKTGKVLGKDYTFVIDRAEAIAEAVRM
                     ARPGDIVLLCSMGCYQCMYVGDGKITWDDRLEARKALEKFQMSKSK"
     gene            7002..>7741
                     /locus_tag="VGA49_01495"
     CDS             7002..>7741
                     /locus_tag="VGA49_01495"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9664476.1"
                     /translation="MPQNPFKNALYQLKSIEEFIPEKDRQYLAYLKQPQRILQVSIPV
                     KMDDGSIKVYEGYRVQYNDARGPFKGGIRYHLQADLNEVKALAFWMAIKCATVNIPFG
                     GAKGGVQVDPKKLSAAELERLTRGYTRAIKDFIGPDKDVPAPDVYTNPQIMAWIMDEF
                     SQIKGYNVPGVVTGKPVEVGGSLGRDTATAQGGFYVLENVVKKLKLNPKKLKIVIQGF
                     GNAGSVMAELVSQAGYQVVGLSDSRGGI"
BASE COUNT         2301 a         1472 c         1776 g         2192 t
ORIGIN      
        1 tttattcagc cttcggctga taaaaatcgg gacagttgta aattaataaa aaaagagttc
       61 cgaagattgt aaattgggcg catagctcag cctggtagag cagttgcctt ttaagcaaac
      121 ggtcggaggt tcgaatcctc ctgcgcccaa tttacaatct tcgggctttt aagttctatc
      181 cgcttttagc ggatcaggac aaacggtcac aggttcgccc gatcattcga tcgggcgccc
      241 ggtcgaatga ccgggcgaat cctgtacgcc ccattttaac cggccaggcc ggtttttttg
      301 ttaggctcat ttatgttata attaaaacaa aaatacaaag tatttatgat ccaaaaagaa
      361 ctctggtaca atttatcagt ttcagaaata ttaaaaaaat ttaaaaccag ccaggctgga
      421 ttaagccaaa aagaagctgt tggcagatta aaaaaatttg gccccaatga actgccccgg
      481 gagaaacgac tgacctggtt taaaattttc ctttcacagt tcaaaagtcc gctggtctat
      541 attttgcttt tggcggcggt tattacctta attcttcagg aatttattga catggccgtg
      601 attatcgcgg ctgtttttat aaatgtcata gttggttttt tccaggaata taaagctgat
      661 caggccttgt ggcagctcaa aaagctgacc aaaattgagg ccaaagtcat tcgtcaggga
      721 attttacacc aaattgactc ggcggaaatc gcgcccggcg atattattat ttttgaagcc
      781 ggcgataaaa ttccggctga tggccggcta atagaggtta ataatttaga gatagctgaa
      841 gcggctttaa ccggcgagtc cgtcccttcc actaaaattg acaacgtttt aaagcggggg
      901 acagccctgg ctgaccggga aaacatggtt tacatgggga cgacggttga gcgcggcaaa
      961 ggccgggcag tcgtcaccgc taccggcgtt cagactgaaa ttggccgaat cgccgtgata
     1021 gtcagggaaa ttcctgaaga aaaaagtcct ctccaaaaaa aattaaaaaa attaaccgcc
     1081 cggctaactt tgattttagg cgtgatttca gccggaattt ttacttttgg ttttttaacc
     1141 ggccagccgt tttttgaaat gctgatcacg accgtcgctc tggcggtggc cgctattccc
     1201 cagggcctgc cggcttcggt ttcaattatt ctggcgatcg gcatgcaaaa gattttaaaa
     1261 agaaaaggac tggtcagaca aatggtcgcc gctgaaactt tgggggcgac gactgtaatt
     1321 tgcactgaca aaacagggac tttaacaaaa ggagtgatga gagtctcgca agttttaacc
     1381 ggcagtcgtg aactgcttca gggaaagcat aaagttttca ctttgccggg ccgggcggag
     1441 gaaatcgggc atatcctggc tttggaaatc agcttattgt gcaataactc tttcattgaa
     1501 aatccggaag atcagctcaa ggactggatt attagagggg actcgacgga aacagcttta
     1561 ttgctggcgg ccgttcaggc cgggttaaac agatcacaac tggaaaaaga gtcgccccgg
     1621 ctggctgaag tcccgtttga ttctgaaaaa aaatacatgg tgacgatgaa taaactggat
     1681 agtcagcata atattttata tttaaaaggc gcccccgaag ttattttaga aatgtcatca
     1741 gcgattaatg ttgatggtaa agaatctaaa ttaactccta ataaaataaa gatcctgaaa
     1801 aagacctatg aagatttgac ccggaaaggt ttaagggttt tgggggtagg ctatagaaaa
     1861 gttaaaattc aaaattctaa agcgctggag gagttatgct gtgattttat ttttgtcggt
     1921 tttatcggac tgaaagatcc tttacggaaa gaagccaaag agactttaag aaaaatttat
     1981 caggccggta tccggccggt ggtggttacc ggcgatcata aattgaccgc ccgggccatt
     2041 tttgaagagc tgggttttcc ggttaagtct gaccggatca tggaaggcgt tgagctggat
     2101 caattaagcg acgctgaact taaacaaaaa ataactgaga tagatgtttt tgcccgggtt
     2161 gagcctaagc ataaaatcag aattgttgac gcctggcagg ccaggggcca ggtcgtggcc
     2221 atgaccggcg acggcgtcaa tgacgctccg gctttgaaat cagcggatat tggggtggct
     2281 ttgggctcgg ggactgatgt cgccaaagaa acagctgata tcgtggtgac tgatgataat
     2341 ttaagcactt tggtggctgc cgtggagcag gggaggataa tttttgaaaa tatccgtaaa
     2401 gtcaccttat attttctgat tgattccttt actgaaatta ttttaatcgg cggcagttta
     2461 ctgctgggtt tgcctttgcc tttactggcc gctcaaattt tatgggtgaa tctagtggaa
     2521 gacagctttc ccagcattgc tttggctttt gaacccggtg agcgggaagt catgaaagac
     2581 cggccgcgga agctgacaga accaatttta gacactgaaa taaaagtttt aatttttgtt
     2641 atcggcataa ttactgattt atttttattt ctgctgttta tctggttatt gggccgcgat
     2701 tattcgatgg aatatatcag aacaattatt tttattactc tggggctgga ctcgttattt
     2761 tatgtttttt cctgcagaag tttccgccac actattttta ccagaagtat ctttaccaat
     2821 aaattattaa tcgcggctgt tttaaccgga atgatgttta tcctgatagg agtttatatg
     2881 ccgattatgc gaaccgtctt aagaacagtt cccctgtcat tttctgactg gctgattccg
     2941 gttgggttgt cagttttagg agttatttta atcgaaatta ccaaagagat ttttatcatc
     3001 agaggccgaa ggaaaaaatt ttcagaagcc gtttattaat attttttaaa tgaaaatagt
     3061 caaaattaac ccggtccggc cgtccccaaa aattattggt caggtcgtaa aaattttaca
     3121 aggcggagga gtggtaatgt ttccgacaga aacagtctat gggctggggg ctgattttaa
     3181 aaatcaagcc ggagtaaaaa aaatttttca aataaaaggc cggcatttaa aaaaagcttt
     3241 gccagtgata gtttccggac tgggccagat taaatcaatt gccgaggttg ataaattagc
     3301 ttataatgtc attaataaat ttttaccggg accgctgact attatattgc caaaaaagaa
     3361 ggtagtttcc ggcttaatca gcgccggtac aaagacagtc gctgtcagat atcccgatca
     3421 ttcggttgct ttaaaattga gttccaggtt ccctttggcc agcagttcag ccaacctgtc
     3481 cggccagccg gcctgttatt cagtcagtca gtttttaaaa caagttaaag gacagcaaaa
     3541 tttgcctgat ttaattttag acgccggccg aattcctaaa gttaagccgt caacaatcgt
     3601 tgatttgacg ggaaagaaaa ttaaaatctt aaggaaaggc cctgtttcca atttccataa
     3661 caaatatgtc caaaaaaaaa ttagtcgttg aaattgtcat tgatggctgg ggaattgacc
     3721 cgtctgaaaa aggcaatgct tttttaatgg ctaaaacgcc ggtttttgat gatttaatca
     3781 gtcattatcc tttcgcggcc attgaggctt ccggtctgga tgttggcctg ccctggggcg
     3841 aggtcggcaa ttcagaagtc ggtcatacca atctgggcgc cggtcaggta atctatcaga
     3901 atttgccccg gattaacatc gccattgaag acaaatcttt tttcagtaat gaagttttcc
     3961 taaaagcagt tcagcacgcc aggaaaaata attctgacct tcatttaacc ggtcttttaa
     4021 gcgacggcgg tgttcacggc cagacagacc atttattcgc tttattgaag ttaataaaag
     4081 agcggaagtt taaacgagtt tttattcacg ccatcactga cggccgggac acggccgctg
     4141 atttggctcc taagtattta aaaaaactgg atgatcagct caaagtcaat ggcctgggac
     4201 ggctggccac aattattggc cgttattacg ccatggatcg tgatgaaaag tgggagaaga
     4261 ctcaaaaagc ctgcgattta ttaacccagg ggctgggtga aaagacccgg gagccgatta
     4321 agaccattga aaaattttac aagaaaaaaa ttgacgacga aatgatggag ccgattgtta
     4381 ttgttgatca ccatgatcag cctgttggtt taattaaaga aaatgactcg gtgatatttt
     4441 ttaattttcg gcctgaccgg gcccgacagc tgactaaagc ctttgttctt gacaatttta
     4501 aaggatttaa aagagataaa cttaaaaatt tatttttcgc gaccatgatg gaatatgtgg
     4561 aaggcttgcc ggttaaggtg gcttttccgc cccagttaat tactgaacct cttggcaaaa
     4621 ttatcgctga tcatggtctg aagcagttaa gaatcgctga aaccgaaaaa tacgcccacg
     4681 tcacttattt tttcaacggc ggcagtgaaa ttcagtataa aaatgaagat cggaaaatta
     4741 ttccttcgcc taccgtaaaa agttttgacc aaaagcctga aatgagcgct tttaaactaa
     4801 cggacgaagt gattgccagg ataaattcaa aaaaatacag ttttatttta atgaattttg
     4861 ccaattttga catggtcggt catactggtc aaataaaagc ggccattagg gcgattgagg
     4921 tcattgacga atgtttgagc cggatcgtgc cggccgtgct tcagcagaac ggtcaggtcg
     4981 ttataacagc cgatcatggc aacgtggaag taatgattga tccggtgacc ggcaaagtca
     5041 ataaatcgca cacgaccagt ccggtgccgc tcttaatcat tgatgaatcc aaaaaagttg
     5101 accggacacc tgacgaagtt gagatgttta aacgcgatat cgcgacaatc ggcattctgt
     5161 ctgacgtgac gccaacagtt cttgaaataa tggggttaaa gcagtctaaa gagatgaccg
     5221 ggagaagctt actcggggac atgacataat aaattctaat atctaaattc tcccgatggc
     5281 gaagccatcg aggatcctcg acaaagtcgg gagaaatcct aaataagcac taatgaccaa
     5341 aattcaaaat atcgcaatgg atcgtttaga atttagatat tagagtttaa gatttattta
     5401 gtatttgtaa ttttggattt ggtgcttact ttttatgata aacactttac gcaaaattac
     5461 gccccggttt ataaaaaatt tttatcataa aaccctggcc ctcctggctt cttttatcta
     5521 tggttttcct tcacgaaaaa tcaagataat cgccattacc ggcacggacg gcaaaaccac
     5581 cacttgttat ttaaccactt caattttgga aacagccggc tataaggtgg ctgaactgtc
     5641 aggcattaga tttaaaatag gtgacaaaga atggcccaat aaaacagaca acaccacgcc
     5701 cggccgtttt aaattaagaa aatttatttc acgagccgtt aaaaacaaat gcgattatct
     5761 tgttttggaa gttacttcct gggctttggc ccagtatcgg gtcaaaaatg tttcaattga
     5821 tatcgctttg ctgacgaatt taactttaga acatcttgat ttgcataaaa ccatggccga
     5881 atacagacaa gccaaaggaa gattgtttga aatgttgtca accggttatt atctggaaaa
     5941 tgttcctaaa atcagcattg ttaatctgga cgacaatgac gctcaatatt ttttgagttt
     6001 taaagctgat cagaaaatta cctatggaat agaaacagcg actgacatca gcgccgaaaa
     6061 tgttaagccc ggggctgata aaattgattt tgatttatta ttaaatgaac aatcggtcag
     6121 ggtgagcttt aaactgccag gactttacaa tgtttataac gctttggcgg cggccgctgt
     6181 tggctcggct ttaaaaatta attcggaaaa aattaaacag ggaattgaaa aaattgaata
     6241 tgtccccggc cggatggagc gaattgacgc cggccaggat tttacagttg tcgttgactt
     6301 tgcccatact cccaacggtc tgaaagaact gtttaaagcg gcccggacaa tgattgagcc
     6361 ggggaaaaaa ataatcgcgg tttacggttc ggccggcggc cgggacaaag aaaagcggcc
     6421 gttgattggc gaagtcgccg gccagctggt tgattttagt tttttaacga cagatgaccc
     6481 gcggaatgaa gatccgttaa aaattgccca gacaattgaa attggcctga aaaaaaccgg
     6541 caaagttttg ggtaaagatt atacttttgt aattgaccgg gctgaagcca tagcagaagc
     6601 ggtaagaatg gcccggccgg gcgatattgt tttattatgc agtatgggct gttatcaatg
     6661 catgtatgtt ggcgacggca aaatcacctg ggacgaccgg ctggaagcga ggaaagcatt
     6721 ggagaaattt caaatgtcaa aatccaaata acaattcaaa tccaaaatct cccgatggcg
     6781 aagccatcga ggatcctcga caaagtcggg acaaatttca aataattttt tgacatttag
     6841 attttgatat tcaattgtca tttgagcttt gtaatttgga tttatttttt tgactaaaat
     6901 tattaagatt tagtatactt aattattgga ttaacttgtc agttttaagt aatttatttg
     6961 aataacttaa atcttaaatc tattaactta aatctatttt tatgccccaa aatccgttta
     7021 aaaacgcttt atatcagcta aaaagcattg aagagtttat tcctgaaaaa gatcggcaat
     7081 atttagctta tttaaaacaa ccccagagaa ttttgcaggt atcaattccg gttaaaatgg
     7141 acgacggatc aattaaagtc tatgagggct accgggttca gtataatgac gcccggggcc
     7201 cttttaaagg cggcattaga tatcatctcc aggctgattt aaatgaagtt aaagctttgg
     7261 ccttttggat ggctattaag tgcgccacgg ttaatattcc ctttggcggc gccaaaggcg
     7321 gtgttcaggt tgaccccaaa aaattatccg cggctgaact ggaaagactg accagagggt
     7381 ataccagagc cattaaagat tttatcggtc cggataaaga tgttccggcg ccggatgttt
     7441 ataccaatcc tcagattatg gcctggatta tggatgagtt tagccagatt aaaggttata
     7501 atgttccggg cgtggtcacc ggcaagccgg tggaagtcgg cggttcgctt ggcagagaca
     7561 cggctaccgc ccagggcgga ttttatgttt tggaaaatgt cgtcaaaaaa cttaaattaa
     7621 atccaaaaaa attaaaaatt gtcattcagg gttttggtaa cgccggatca gtcatggctg
     7681 aactggtcag tcaggccggc tatcaggttg ttggtttgtc agattccaga ggcggtattt
     7741 a
//
LOCUS       DASWHZ010000066         4971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_886298, whole genome shotgun sequence.
ACCESSION   DASWHZ010000066 DASWHZ010000000
VERSION     DASWHZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886298"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VGA49_01500"
     CDS             complement(<1..641)
                     /locus_tag="VGA49_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX9664477.1"
                     /translation="MVDISKFKEIFVSEAEDLLMNLNKDLLALEKKGAQKNLLNNLMR
                     SFHTLKSSAAAMGYKQISSLAHSLEDIFDQTRKTGAKLGQVVISRSFQAVDALEKSLG
                     EIKKSGQEADLSSVIAGVQKISSQSSKKKAGFTPAVKSAATVTESQTVEAISHVKVPV
                     QRLDQLLASVEELLIDKLKLERLVKFEKVNQLELRDITAHLSRLVSDIQYQVM"
     gene            complement(643..1707)
                     /locus_tag="VGA49_01505"
     CDS             complement(643..1707)
                     /locus_tag="VGA49_01505"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX9664478.1"
                     /translation="MKIRVLIVDDSFFMRKLIAELLSEHPKIEVIDDASDGPEAVEKA
                     VKLKPNVITMDYRMPGLDGAKATEKILAKVEPPPAIIMLSAYTKEGAEETLNSLKAGA
                     VDFIAKPSGELSLDINKIKQELVTKVIMAAQARVIRRTIKSVPKKVESRERQPVKRTS
                     AAKVIVLGASTGGPPIIEDILVGLPANLNSSVLIVQHMPEHFTESFARRLAAMSNLQV
                     REAANNDVIKTSLAFVARGGYHMIIKKRTAGQNIERLINLTQAIPEWKGFRPSIDILM
                     ESVAENFGREVVGVLLTGMGEDGRQGMRSIKEARGYTIVQEPDTAVVDSMPKSVIKDG
                     VADEVLPPDHIARRIIELVQ"
     gene            complement(1704..2063)
                     /locus_tag="VGA49_01510"
     CDS             complement(1704..2063)
                     /locus_tag="VGA49_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9664479.1"
                     /translation="MNKIKILIADDSAFMRRVLKGILEKGGYTNTVEAENGRQALEKF
                     KAEKPDLILLDIVMPEVDGIEVLKKIGSKANIIVISAVGQDVIMEDAKKSGAKGYIVK
                     PFDEKEVLEEVNKVAPR"
     gene            complement(2068..2634)
                     /locus_tag="VGA49_01515"
     CDS             complement(2068..2634)
                     /locus_tag="VGA49_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9664480.1"
                     /translation="MKSQIKNIIKKVAEESVASQKKPEALVQLVVFELDQEEYAVQIT
                     DIREIIKFPLITPIPNAPAFISGIFNLRGKIIVTVDMEKRFHLVREKVITPKHIIITE
                     VNGGAYGVIVDQVTEVLRVPVISIQSAPELVSSKIHADYLKGVVVLENDKRSRLIILL
                     DFPKLLQEKELLSFGKIVNNTQKIISKS"
     gene            complement(2631..4334)
                     /locus_tag="VGA49_01520"
     CDS             complement(2631..4334)
                     /locus_tag="VGA49_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9664481.1"
                     /translation="MNLFKNLTVGKRVALGAMALIVLIILGTFFSIRSLTSIERLSAG
                     TARIVRTTEDALLLDIYFLRNRVDLNNYIDTADPAFIKEIQENRTFIEQTFKELLDQE
                     KNPALLKSLDEFESIRSRSLELADQIIAAVNSQAPEEQINLLFAESRGLSNKSREILS
                     NIIDFERKAFSAALEQTASRINNLKIENFIFFGGVIFIALIIFIILFRSTVPVIVKAV
                     DRTSKAASGLASSSQQAASASQQVASVSQQVASGATQQSQQAEEVSKSLAQMAANIQQ
                     MSASSQEVSATSTKVAGMAQIAGEKGEQSQKGLTKINAIIGDASNMIKTVSGRTDDIR
                     KIVEIITSIAEQTNLLALNAAIEAARAGEAGRGFAVVADEVRKLAEGASKASAEIKDK
                     IKEMISQIQNTVNSVEGGKKTAEESGKIISDTLSNLQEIAAGIQQVSSKTQELSAAVT
                     QQSSSVQQVAKTMDSIAAVAVQNASGAQQLSASTQQQSAANQQVASTAQELLSIARRF
                     AALVGSKIDEAGLPSRKPMDELAVEQGFEPTEVLEPRPESIKTKPAKVKRQPIKKKKQ
                     S"
     gene            complement(4571..4879)
                     /locus_tag="VGA49_01525"
     CDS             complement(4571..4879)
                     /locus_tag="VGA49_01525"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9664482.1"
                     /translation="MTKSTTTTDLRRILKSRKVKESYTILRVLSGITRFKIVLILNYR
                     ERGLTVSEIASVLDMSLPSVSHQLKILRKHKVVTGRGMNREVVYALNNHHLRKILSFS
                     "
BASE COUNT         1252 a         1198 c         1011 g         1510 t
ORIGIN      
        1 tgcattacct gatactgaat gtctgatacc agccggctca aatgcgcggt aatatcgcgc
       61 agctccaact gatttacttt ttcaaatttg accaggcgct caagctttaa tttgtcaatt
      121 aacagctcct cgactgacgc cagcagctga tcaagccttt gaaccggcac tttgacatga
      181 gaaatagctt caacggtttg ggattcggtc accgtggccg ccgatttaac agcaggcgtg
      241 aaaccggctt tcttctttga ggattggctg cttatttttt gaacacctgc aatgaccgag
      301 cttaaatctg cttcctggcc cgatttttta atttctccca aagatttttc taacgcgtca
      361 actgcctgga aagaccgact aatcacgacc tggccaagct tagcgccggt ttttcgggtc
      421 tgatcaaaaa tatcttctaa agaatgggcc agcgaggaga tctgcttata tcccatggcc
      481 gcggccgacg actttaaagt gtgaaaagaa cgcattaaat tattgagcaa atttttttgg
      541 gcgccttttt tttcaagcgc cagaaggtct ttgttcaaat tcattaaaag atcttccgcc
      601 tcgctaacaa atatttcttt aaatttactt atgtccacca ttttactgaa ctaattcaat
      661 aattctcctg gcaatgtgat ctggcggcaa aacttcatca gccacgccgt ctttaatcac
      721 ggattttggc atggaatcaa ccacggccgt gtcaggttcc tgcactatcg tataacctcg
      781 cgcctcctta attgacctca tcccctggcg cccatcctct cccatgccag tcaacagcac
      841 gccgaccact tctctgccga aattttccgc cactgactcc atcaaaatat caattgacgg
      901 cctaaaccct ttccattcag gaatagcctg ggtcaagtta atcagtctct cgatattttg
      961 gccggctgtc ctctttttta taatcatgtg atatccgcct cgcgccacaa aggccagact
     1021 ggtttttatg acgtcgttat tggccgcttc cctaacttgc aaattagaca tggccgcgag
     1081 gcgcctggcg aaactttcag tgaaatgttc aggcatgtgc tgaacaatga gaaccgagga
     1141 atttaaattg gccggcagtc ccactaatat atcttctatg attggcgggc cgcctgttga
     1201 agcgcccaag acaattacct tggccgcgct tgtccgcttt actggctggc gctcccgact
     1261 ttccactttt ttagggacag actttatggt tcgcctaatt actctggcct gtgcggccat
     1321 tattacctta gtcaccagct cctgctttat cttgttaata tccaaagaca attcacccga
     1381 tggctttgcg ataaaatcca cggcgccggc cttgagacta tttagagttt cttcagcgcc
     1441 ttccttcgta taagctgaca acattattat ggctggcggc ggttcaactt tggcaagaat
     1501 tttttccgtc gccttggcgc cgtcaagtcc aggcatccga taatccatcg taatcacgtt
     1561 aggctttaat ttgaccgcct tttccactgc ctccgggccg tcgctggcgt catcaatcac
     1621 ttcaattttt ggatgctcac ttaaaagctc ggcaatcaat ttgcgcatga aaaacgaatc
     1681 atcaacaatg agcactctta ttttcatcgg ggagccacct tatttacttc ttcaagcact
     1741 tctttttcgt caaaaggctt tacaatgtag cctttggcgc cgcttttctt ggcatcctcc
     1801 atgattacat cctgaccgac ggcgctaatg acaataatat tggccttgga gccaattttt
     1861 tttaaaacct ctatcccatc cacttccggc atgacaatat caagcaaaat taaatcaggt
     1921 ttttctgctt tgaatttttc caaagcctgt cgaccgttct ccgcctcaac ggtattggtg
     1981 tatcctcctt tttcaaggat tcctttcagc accctcctca tgaaagccga gtcatcggca
     2041 atcaaaattt taattttgtt cataaattta ggacttagaa attatctttt gagtgttatt
     2101 gactatcttt ccaaaggaca aaagctcttt ttcctgcaaa agctttggga aatccaataa
     2161 aattattaag cgagatcttt tatcattttc taaaaccacc acgcctttta aataatcagc
     2221 gtgaattttg gatgaaacta attctggcgc cgactgtatg gaaataaccg gcacccgcaa
     2281 aacttcagtt acctgatcaa ctattacgcc gtaagcgccg ccattaactt cagtaataat
     2341 gatatgtttg ggcgtgatga ctttttcccg caccaaatga aaacgttttt ccatgtccac
     2401 ggttacgatg atttttcctc tcaaattaaa tatcccactg ataaacgcgg gcgcgttggg
     2461 aataggggta atcaatggaa acttaataat ttctcttata tccgtaattt ggacggcgta
     2521 ctcttcttga tctaattcaa acaccaccag ttgcactaat gcttccggct ttttttgcga
     2581 ggccactgat tcttcggcga ctttttttat tatgtttttt atctgactct tcatgactgc
     2641 ttctttttct ttattggctg gcgcttgact ttggcaggct ttgtttttat gctttccggt
     2701 ctgggctcga gaacctcggt tggttcaaag ccttgttcaa cagctaattc atccatgggc
     2761 ttgcgcgaag gcaaacccgc ttcatcgatt ttggacccga ccaaagcggc gaaacgcctg
     2821 gctatggaaa gtaattcttg agcggtagac gcgacttgct gattagcggc gctctgctgc
     2881 tgggtggacg cggaaagttg ttgagcgcca gacgcgtttt gaacggccac cgcggcgatt
     2941 gagtccattg ttttagccac ttgctgaact gaagatgatt gctgggtcac ggccgctgac
     3001 aattcttgag ttttacttga cacctgctga atcccggccg cgatttcctg caggtttgaa
     3061 agggtgtcac tgatgatttt gccgctctct tcagcagttt ttttaccgcc ttcaaccgaa
     3121 ttaaccgtgt tttgaatctg actaatcatt tcttttatct tatctttaat ctcggctgat
     3181 gctttgctgg ccccttcggc caactttctg acttcatcag ccaccaccgc gaagccgcgt
     3241 ccggcctcgc cggcccgggc cgcttcaatg gcagcgttta aagccaacaa gtttgtttgc
     3301 tcggcaatgg aagtaataat ttcgacaatt tttctgatgt cgtcggttct tccggaaaca
     3361 gttttgatca tgttcgaagc gtcaccgatt atcgcgttta ttttggttaa tcctttttgc
     3421 gactgctccc ctttttcacc ggcaatctgg gccatgccag ccactttggt tgaggtagcc
     3481 gatacttctt gagaagaagc ggacatctgt tgaatgtttg ccgccatttg ggctaacgac
     3541 tttgagacct cttccgcttg ttgcgattgt tgggtggcgc cggaggcgac ttgttgcgag
     3601 accgaagcga cttgttgcga ggccgaagcg gcctgctgcg atgaagaggc caggccactg
     3661 gctgcttttg aagtccgatc aaccgcctta acgataactg gcaccgtgga gcggaaaagt
     3721 atgataaata ttatcaaagc gataaaaatg acgcccccga agaatataaa attttcaatt
     3781 tttaaattat ttattcgcga ggcagtttgc tccagcgccg cgctaaaggc ctttctttca
     3841 aaatcaataa tattgcttag aatttcccgg gatttattgg atagcccgcg gctttcggca
     3901 aacagaagat tgatctgctc ctctggcgct tgtgaattca ccgccgcgat tatctgatca
     3961 gctaattcca aactccgaga tcgtattgac tcgaattcgt caagtgattt tagcaaggca
     4021 ggattctttt cctgatccag caattcctta aacgtctgtt ctataaatgt gcgattttct
     4081 tgaatctctt taatgaatgc cggatcggct gtatcaatat aattatttaa atcaaccctg
     4141 ttacgcaaaa aataaatatc caggagaagc gcgtcctcag tcgtccgtac aatcctagcc
     4201 gtgccagcgc ttagccgctc tattgaagta agggaacgaa tagaaaaaaa tgtgcctaat
     4261 attattagaa caatcaacgc catggcgcct aaagccaccc ttttgccaac agtcaaattt
     4321 ttgaataaat tcatattttt aagctccttt tattttttaa tacttgaaaa aaaatgcaag
     4381 tattattatt ttattataac acactaaaaa atagttggta agtgcaaaaa agggcggccg
     4441 cgatgaggcc ggcgacgaat accctaaaac ccgcaatttt aaaagccatc ttccgactga
     4501 cggatggatg gcctcagccg acaaggtggc tatttttcgc gaaaaaacct caaatttatt
     4561 gaggaattat tcagctaaaa gacaaaattt ttcttaaatg atgattgttg agggcataaa
     4621 ctacttcccg gttcatcccc ctgccagtca cgactttgtg tttacgaaga atttttaact
     4681 gatgagagac gcttggaaga gacatatcca gcacactggc aatttccgat acggtcaagc
     4741 cgcgttcgcg ataatttagg attaaaacaa ttttaaacct ggtgattccg gacaatacac
     4801 gaagaatcgt gtaagactcc ttaaccttac gacttttaag aattcggcgc agatcagttg
     4861 tagtagttga tttggtcatt ttgatttata tcttctctct ataatttttt aacataataa
     4921 atattgcaaa ctccgggggt gggattcgaa cccacgacca attggttaac a
//
LOCUS       DASWHZ010000067         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_145944, whole genome shotgun sequence.
ACCESSION   DASWHZ010000067 DASWHZ010000000
VERSION     DASWHZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145944"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            536..937
                     /gene="rnpB"
                     /locus_tag="VGA49_01530"
     ncRNA           536..937
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGA49_01530"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            947..>2077
                     /locus_tag="VGA49_01535"
     CDS             947..>2077
                     /locus_tag="VGA49_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9664483.1"
                     /translation="MNKVELIFKAILVPLDFVMLVLAGLAAYSIRFQTFVTEIRPVVY
                     EMPFKGYLNIVLIMATLWVVIFALEGLYTIKGPRKIIHELMKVFFACSTGLIIIIILI
                     FLERELFSSRFIILAAWGLSVVLVSLGRILIRITRLILLKYGIGARKIILVGKNKSTK
                     AITESIKHSQRLGYKIVEHFDLVNAETLEKMKKLIADGRVEEILQSDPDMNKENVLDL
                     IDLANEYQISFKYAADLFETKATNIEVTTIAGIPIIELKKTPLEGWGRIVKRLFDIIC
                     SLIFIILTSPILAIVAIAIKADSRGPIFYLNERVTKGGKTFEVFKFRTMKAEYSTGPR
                     YDKTGEAAKLEKKLIEEKSQRVGPVYKVLKDPRRTSLGRLLEK"
BASE COUNT          624 a          392 c          460 g          601 t
ORIGIN      
        1 aagggattat caggggcggg ataaaattgt ggtgaagtgg cccgacaaaa agaaagtttt
       61 gattatggaa gaaaatagag atttttatgg tgatcttatt aaaattttga aaagggggtg
      121 aaagtacaaa gtaagtacaa agtacaaagt gcaaagtgca aagcaagtcc aaagtccaaa
      181 gaaagttcaa agtacaaagt ccaaagttca aagtacaaag caagttcaaa tttcaaagcc
      241 cctgatttcc cttgatgtcc cctgataatc cctgatctcc cctgaaacaa aaaaagacct
      301 acattaaata tgtaagtctc atgtccctcc attctggaca gcaggcttta gcctgcatgg
      361 cccgcacccc tctttttgag gtgttttggc acctttagct ttgagcttga tttgatcttt
      421 gaactttgaa ctttgatatt ttaaccttca tctcccctca tctcccctta tctcccctca
      481 tctaccctgc ttgccgtttg acaaaaccat cattttccgc tacaatataa ttatccaggc
      541 aagctggatg attgctagaa atgccctttt gaagcccttc gataagggga tttttagagg
      601 aaagtccgaa cacccctccg atgtccatcg gaggaagcaa ggcagttcct aacgggaacc
      661 gggagagtag ggagtagaag ttctatagac ttttgctgtt tatgaatccc agggcaagtg
      721 caacagaaaa gacacatccg atggcccctt ggggcacagg agaagctgaa atcgtgaggt
      781 aagagctcac gcgtgaaaaa ttccgtggtg acacggcacg tggtaaacac ctgcctggtg
      841 caagagtcag gcgactcgct ggagtctgat cgtgaaatca gacctagata gataatcatc
      901 ttcaaaacag aattcggctt acaggcttgc ctggattttt atatttatga acaaagttga
      961 attaattttt aaggcaattt tagtgccgct ggattttgtc atgctggttc tggccggcct
     1021 ggccgcttat tcaatcagat ttcagacttt tgtgactgaa atccggccgg tcgtctatga
     1081 aatgcctttt aagggatatt tgaatatagt tttgataatg gccaccctct gggtggttat
     1141 ttttgctttg gaggggcttt acacgattaa aggaccccgt aagatcatcc atgaattgat
     1201 gaaagtcttt tttgcctgtt caaccggcct gattataatt attattttaa tttttttaga
     1261 aagagaactt ttttcttcca gattcattat tttagccgcc tggggcttaa gcgttgtttt
     1321 agtttcactg ggcaggattt taatcagaat taccaggttg attttgttaa aatacgggat
     1381 tggcgccagg aaaataattc tggttggtaa aaataaatca actaaagcga ttactgaatc
     1441 aattaaacat agtcagcggc tgggctataa aattgttgaa cattttgatc tggtcaacgc
     1501 tgaaactttg gaaaaaatga aaaaattgat agctgacggg cgggttgaag aaattcttca
     1561 gtcagatcct gatatgaata aagaaaatgt tttagatttg atagatttag ccaatgaata
     1621 tcagataagc tttaaatacg ccgctgattt gtttgaaacc aaagcgacca acattgaagt
     1681 gacgaccatt gccggcattc cgattattga actcaaaaaa actccgctgg agggctgggg
     1741 acggattgtt aaaagacttt ttgatattat ctgttccttg atttttatta ttttaacttc
     1801 gccgatttta gcgatagtcg ccattgccat taaggctgat tccagggggc caattttcta
     1861 tttaaatgaa agggtcacta aaggcggcaa gacttttgag gtttttaaat tcagaacaat
     1921 gaaagctgaa tacagcaccg gccccagata tgacaagaca ggggaggcgg ctaagcttga
     1981 aaagaaatta attgaggaaa aaagccagag agtcggcccg gtttataagg ttttaaagga
     2041 tccccgccgg acaagcctcg gccgtttact ggaaaaa
//
LOCUS       DASWHZ010000068         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_533758, whole genome shotgun sequence.
ACCESSION   DASWHZ010000068 DASWHZ010000000
VERSION     DASWHZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533758"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..226
                     /locus_tag="VGA49_01540"
     CDS             86..226
                     /locus_tag="VGA49_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664484.1"
                     /translation="MIAEKIQLSRGVRKYIRKEKARIRREVLDFEEQTRRIEELYKKF
                     SN"
     gene            complement(269..1093)
                     /locus_tag="VGA49_01545"
     CDS             complement(269..1093)
                     /locus_tag="VGA49_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX9664485.1"
                     /translation="MPAKMIDGQKLAEAIKKEVGKEIVEKHLQPGLAIILIGDDPASH
                     LYVKLKKKACQQVGIEFHKYYCSGQSCLKEVPEAIKFLNKDPAINAILIQLPLPKEFD
                     ENATIKLMDPKKDVDGFHPDQTFLTPGLAGGILNLIKSTKENLAGKKAVIVSNSPVFA
                     GPIKKILDEQEVKTEAYLADEKNLGDKASQADILIVAVGRPNFITEEMVKQNSIVIDV
                     GTNRQAGTIVGDVDFNSVKNKAGWLTPVPGGVGPMTIAMLLKNTVELTKKQKAKNF"
     gene            complement(1083..2342)
                     /gene="glyA"
                     /locus_tag="VGA49_01550"
     CDS             complement(1083..2342)
                     /gene="glyA"
                     /locus_tag="VGA49_01550"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017691928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9664486.1"
                     /translation="MKILHNQDPKIAQAIFNELKRQQEGLELIPSENFVSLAVLEALG
                     SVLTNKYSEGYPGKRYYGGTEHVDVIEQLAIDRAKELFGAEHANVQPLSGAPANIAVY
                     SALLEPGDTVLGMDLSHGGHLTHGHPVTLMAKIYNFVRYKTDKETGLIDLNNLRAMAL
                     EHKPKLILAGYSAYSREIDYQGVKKIADEVGALTMADVAHIAGLIAGGVMNNPVPLFD
                     VVTSTTHKTLRGPRGGLILCKEKQAAAIDKAVFPGLQGGPHEHNIAAKAVAFGEASRP
                     EFKDYCQQIKKNATALCDAMRSEGFGICFGQTENHLVLADITPFDIGGKQAQNLFDSV
                     NITLNKNMIPDDPRSPFDPSGIRVGAPALTTRGFREDDFKIVGQLLGKTIKERNNQTA
                     LNEVRATVKELTDKYPLYPELTEKYAG"
     gene            complement(2530..4167)
                     /locus_tag="VGA49_01555"
     CDS             complement(2530..4167)
                     /locus_tag="VGA49_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HEX9664487.1"
                     /translation="MKKILAQITQDLIEAKPANRQDLLKIVRRGAGKFKIDQPRNSSL
                     LKTYHTLLKNKTIEADKNLEGLLKKAEIRTLSGVAPITVLTKPAGCRNACVYCPTEAR
                     MPKSYLSNEPAVMRAILSKWDPYVQMKVRIRALKENGHATDKLEVIVIGGTWSYHQRN
                     YQNWYIKRLYDGANSSPLLPYDAPFQKEKGARTLAEAQKINETADNRIIGLTLETRPN
                     CITPQELRRFRKFGCTKVEMGVQHLDNKVLKLINRGHDVEESIRATKLLKDAGFKICY
                     HMMPGLPGSTPEKDVENFKTLFSDQRFQPDMLKVYPTAVTKNAVLYKWLQEGKYKPYT
                     KEERFEMLLQIKEAVPYHCRIIRLIRDIPSVSIVAGNKVTNLREMLQQQLASQGKSCK
                     CIRCREARNRTTNLNEAVLVTRKYQASDGIEYFISYESRDNKILYAFVRLRIPSHVPQ
                     NKPDDSELLKLLPDLKGAALIRELHTYGKLVPLTSEKSEAVQHAGFGRRLMAEAERIV
                     KDLGIKKIAVISGIGVRGYYEKLGYEVEDTYMTKEIT"
     gene            4240..>4827
                     /locus_tag="VGA49_01560"
     CDS             4240..>4827
                     /locus_tag="VGA49_01560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9664488.1"
                     /translation="MNQTTAEKLVKKVKDDYVRIADEFSESRSSLWPEMTDFLTYIKD
                     GDQILDLGCGNGRLCQLFRDKKISYLGIDNSPELIADASQKFTNYQSKCQFEVGDILN
                     ISQQSTVNSQQYNVIFMIAVLQHIPSKKLQLKVLRDVNKLLKKDGYLIMTNWNLYRRK
                     YWSYLFRKYFSFKSLKLTGWDIKDALIPWKGKNETV"
BASE COUNT         1271 a         1013 c         1089 g         1454 t
ORIGIN      
        1 atttatttag tatttcgaat tttgaatttg gtgcttttga atgacccttg catcttttac
       61 ccgtttcccc tataatagtg agactatgat tgctgaaaaa atacaattaa gccgcggtgt
      121 cagaaaatat attcggaaag aaaaagcccg gatcaggcgg gaagttttgg attttgagga
      181 acaaacaagg cggattgaag agctttataa aaagttttca aattagacat tagacattag
      241 atattgatta gaaattagtg cttagaaatt agaaattttt agctttttgt ttcttcgtca
      301 gctcaaccgt gtttttcagc agcatcgcga tcgtcatcgg accgacgccg cccggcaccg
      361 gcgtcagcca gccggcttta tttttgacag aattaaaatc aacatcgccg acaatagttc
      421 cagcctgccg gttggtgccg acatcaataa caatactgtt ctgtttgacc atttcttcag
      481 ttatgaaatt cggccggcca accgccacga ttaaaatatc cgcctggctg gctttatctc
      541 caagattttt ttcgtcagcc agataagctt cagttttaac ttcctgctca tccaggattt
      601 ttttaattgg cccggcgaaa accgggctgt tggaaacaat aacagctttt ttgccggcca
      661 ggttttcttt agttgattta atcagattga ggatgcctcc ggccaggccc ggagttaaaa
      721 atgtctgatc aggatgaaag ccgtcaacat cttttttagg atccattaat ttaatagtgg
      781 cgttttcatc aaattctttc ggcaaaggca gctggattaa aatggcgttg attgccgggt
      841 ctttatttaa aaatttaatg gcttcaggaa cttctttcag gcagctttga ccggaacagt
      901 aatatttatg aaattcaatg ccgacctgct gacaagcttt ttttttaagc ttaacgtaaa
      961 gatgagaagc cggatcgtcg ccaatcagaa taatcgccag gccgggctga aggtgttttt
     1021 caacaatttc tttgccgact tcttttttaa tcgcttcggc cagtttttgg ccgtcaatca
     1081 ttttagccgg catatttttc agttaattcc ggataaagcg gatatttatc cgttaattct
     1141 ttgacggtgg ccctaacttc gttaagagca gtttgattat ttctttcttt gattgttttg
     1201 ccaagaagct gaccaacaat tttaaaatcg tcttcccgga agccgcgggt cgttaaagcc
     1261 ggggcgccga ctctgatgcc tgacgggtca aacggcgacc ggggatcatc cggaatcata
     1321 tttttattga gagtgatatt gactgaatca aataaattct gggcttgttt gccgccaata
     1381 tcaaaaggcg taatatcagc caggaccaga tggttttcag tctggccaaa acagatccca
     1441 aagccttctg atctcatggc gtcgcacaga gccgtggcgt tcttttttat ttgctggcag
     1501 taatctttaa attccggccg cgaagcctcg ccaaaagcga ccgctttggc ggcaatatta
     1561 tgttcatggg ggccgccctg gaggccgggg aagaccgctt tgtcaatggc cgcggcctgt
     1621 ttttctttgc aaagaattaa gccgccccgc ggcccccgta aagttttatg agtggtggaa
     1681 gtgaccacgt caaaaagtgg caccgggtta ttcatcaccc cgccggcgat taaaccagcg
     1741 atatgagcga catcagccat agtcagagcg ccaacttcat cagcgatttt ttttacgccc
     1801 tgataatcaa tttcccggga ataagctgaa tagccggcta aaattaattt tggtttatgc
     1861 tccagagcca tcgcccgtaa attgttcaga tcaatcaggc cggtttcttt atctgtttta
     1921 tagcggacaa aattgtaaat tttagccatc aaagttaccg ggtggccgtg agtcaggtgg
     1981 ccgccatgcg acaaatccat gcccagtact gtatcgcccg gttccagtaa agccgaataa
     2041 acggcgatat tggccggcgc tccggacaaa ggctggacat tggcgtgttc ggctccaaaa
     2101 agttctttgg cccggtcaat cgccagttgc tcaatgacat caacgtgttc agtgccgccg
     2161 tagtagcgct tgcccggata gccttcagaa tatttattgg ttaaaacgct tcccagggct
     2221 tccaaaacag ccagggaaac aaagttttcc gaaggaatca gttccaggcc ctcctgctgc
     2281 ctttttagtt cattaaaaat agcctgggcg atttttgggt cttgattgtg gagaattttc
     2341 ataagagaaa ggattaagaa ttaattaagg cacaaggcac aaggcaaaag gcacaaggca
     2401 caagggttgg tgtcccccac cttaggcggg ggacagatta ttagaaaatc tattgcccga
     2461 tccccattaa aaaattgtca ggcaacagcc tgacacaatc cctgttgcct tgtgcctatt
     2521 gcccgttgcc tatgttatct ctttagtcat ataagtatct tcaacttcgt agccgagttt
     2581 ttcataatag cccctgacgc cgatgccgga gataacggct atttttttaa tacctaaatc
     2641 tttaacaatt ctttcggctt cagccattaa ccggcggcca aagccggcgt gctggacagc
     2701 ttcagatttt tcagaagtta aagggaccag tttgccgtaa gtgtgcagtt ctctgattag
     2761 agcggcgcct tttaaatcag gcaaaagttt caggagctct gaatcgtctg gtttgttttg
     2821 cgggacatga gaggggattc ttaaacgaac gaaagcgtat aagattttat tgtcccggct
     2881 ttcgtaggaa ataaaatatt caatcccgtc agaagcctga tattttctgg taacaaggac
     2941 agcttcattc aaattagttg tccggttgcg ggcttcccgg cagcggatgc acttgcatga
     3001 ttttccctgg ctggccagct gctgttgaag catttctcgc aggttggtaa ctttattacc
     3061 agcgacaatg ctgacgctcg ggatgtccct gatcaaacgg ataatccggc agtggtaagg
     3121 cactgcttct ttaatctgga gcagcatttc aaatctttct tctttggtat aaggtttata
     3181 tttgccttcc tgaagccatt tgtataaaac cgcgtttttg gtaacggctg tcggataaac
     3241 tttgagcatg tcgggctgga aacgctggtc gctgaacaga gttttaaaat tttcgacgtc
     3301 tttttccggg gttgagccgg gcaggccggg catcatgtga taacagattt taaagccggc
     3361 gtcttttagc aatttggtcg ccctgataga ttcttccacg tcatgaccgc ggttaatcag
     3421 ctttaaaact ttattatcaa gatgctgaac ccccatttcc actttggtgc agccaaattt
     3481 tctgaatcgc cggagttcct ggggagtgat gcagttggga cgagtttcca aagttaaacc
     3541 aataattctg ttatcagcgg tttcattaat tttttgagcc tctgccagcg tccgggctcc
     3601 tttttccttt tgaaagggcg cgtcataagg gagtaatgga ctggaattag ccccgtcata
     3661 taatcttttg atataccagt tttgataatt cctttgatgg taggaccagg tgccgccaat
     3721 cactatcact tccagcttgt cagtggcgtg gccgttttct tttaaagctc tgattctgac
     3781 tttcatttga acataaggat cccatttgct caaaatagcc cgcatgaccg ccggttcatt
     3841 ggacagatag cttttaggca tccgggcttc agtcggacag tagacgcagg cgttgcggca
     3901 gccggccggt ttggttaaaa cggtaatcgg ggccacgccg gacaaagttc tgatttcagc
     3961 ttttttgagt aaaccttcca gatttttatc agcttcaatt gttttgtttt ttaaaagggt
     4021 gtgataagtt ttaagaagag aactgttgcg gggctgatca attttaaatt taccggctcc
     4081 ccgccggacg attttaagca gatcctgccg gttagccggc ttggcttcaa ttaaatcttg
     4141 ggtgatttga gctaaaattt ttttcatttg agatttaagt tgaaagattt aagatttcag
     4201 taactttaat cttaaatctt aaatctttaa tctattctta tgaatcaaac aactgccgaa
     4261 aaattagtta aaaaagttaa agatgattat gtaaggattg ctgatgaatt ttcggaaagt
     4321 cgctcaagtt tatggccgga gatgacagat tttttgactt atattaagga tggcgaccag
     4381 attttagatc tgggctgcgg caatggccgg ctctgccagc tttttaggga taagaaaatt
     4441 agttatttgg gaatagataa cagccccgaa ttaatagccg atgcttccca aaagtttact
     4501 aattatcaga gcaagtgtca atttgaagta ggggatattt tgaatatcag tcaacagtca
     4561 acagtcaaca gccaacagta caatgtaatc tttatgatcg cggttttgca gcatattccg
     4621 tcaaagaaat tacaactgaa agttttgcgg gacgtcaaca agcttttgaa aaaagacggc
     4681 tatttgatta tgaccaactg gaatttatac cggaggaaat attggtctta tttatttaga
     4741 aaatattttt catttaaaag tttaaaactg accggctggg atattaaaga cgccctaatt
     4801 ccctggaaag gaaaaaatga aacagtt
//
LOCUS       DASWHZ010000069         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1262113, whole genome shotgun sequence.
ACCESSION   DASWHZ010000069 DASWHZ010000000
VERSION     DASWHZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262113"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VGA49_01565"
     CDS             <1..2066
                     /locus_tag="VGA49_01565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX9664489.1"
                     /translation="EYQLNQDEAIKKAAEKKGIDLSHFKKIDKQLKASGQGSAIIEHI
                     HQKTIDDEIRAVVTKAIELYNNDQESSWADFAILIRANSQADKFIRVLSQARIPYQFM
                     ASKGLYSKPVILDILAYLKLLDNYHESPALYRVLNFSFLKINAQELIKIIHYARRKSI
                     SLYEALRQAGLIGLSRDTLSVLAGLLSLVEKHTALAREKNVLEVFYTALKDLGYIQYL
                     EKEDQAAAWQLNRESISYLNQFIKQIQNFEADNDSKSVKIFLEYMDFVIESGDIGQLS
                     PDFEIGPETLKILTVHAAKGLEFKYVFIANLVDRRFPTIDRKDPIEIPDELVKEILPG
                     GDFHLEEERRLFYVAMTRAKEHLYFSSASDYGGARKKKLSRFLQELNDQGFPINQAEE
                     VSARGGSASGGSLPSFRLFQPVTPIEKEVKQQAEIIPDKFSYTQLKIFQTCPWQYRYQ
                     FVLKIPTFGKATFSFGKTIHGALQKFFQNIVEGKIARQTNLFGKKSGVGTGKLPSWDD
                     LLKLYEENWIDEWYADQDQKKKYKEEGRAILKNFYGEVEKEMPNVVELEKGFNIKIND
                     YTIKGQIDRIDLKADGGIEIIDYKTGNIPDAKYLDKDQLLIYQLAASEIFGKNVSNLT
                     YYYLNKKGNQKLSFLGADKDLDKIKAKVSQIIEDIKTSNFTPAPDKMKCKYCDYKDIC
                     EYRII"
     gene            2083..2373
                     /locus_tag="VGA49_01570"
     CDS             2083..2373
                     /locus_tag="VGA49_01570"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX9664490.1"
                     /translation="MLHINKILKNSIKKSGLEPQIEASLVLEEAGQILYAIFGDKIVN
                     QARPLYVKNKILTIAVLSSIVSQELKLRESEIIDKINKKFEKNLVARLRFAA"
     gene            2549..2971
                     /locus_tag="VGA49_01575"
     CDS             2549..2971
                     /locus_tag="VGA49_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664491.1"
                     /translation="MTLRQYLTLMIISTLIFWAAFLMVLLNIDPGQTGFFGFLFFYLS
                     LFLALTGTIALVGLLIRSYTLKHEMIFRLVTRSFRQGLFFSVLIIGTLLLQSQRLLTW
                     WNTIFLVIGLTALEFFFVSDKRRRVPLRENYFDQEDIV"
     gene            3088..>3290
                     /locus_tag="VGA49_01580"
     CDS             3088..>3290
                     /locus_tag="VGA49_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664492.1"
                     /translation="MILNLFYGIVDYLTGNGVTEAALYHLSYGLTGAGYFDYWKMIII
                     TVSVILMALVLFFWLFFRNPVPQ"
BASE COUNT         1011 a          553 c          694 g         1032 t
ORIGIN      
        1 tggaatacca gcttaatcag gacgaagcaa ttaagaaagc cgctgaaaaa aaggggattg
       61 acctgtcgca ttttaaaaaa atagacaaac aattaaaagc tagcggccag gggtcggcca
      121 taattgagca tattcatcaa aaaacaattg acgatgaaat cagagcagta gtgactaaag
      181 cgattgaact ttataataat gatcaggagt ccagctgggc tgatttcgcg attttgattc
      241 gggccaacag ccaggctgac aagtttatcc gggttttaag ccaggcccgg atcccatatc
      301 agttcatggc atccaaaggg ttatattcca aaccggttat tctggatatt ttagcttatt
      361 taaaattact cgataattat catgaaagcc cggccttgta tcgggtattg aatttttctt
      421 ttttaaaaat taatgcccag gaactgatta aaataattca ttacgcccgc cggaaatcga
      481 tttccttata tgaagctctc aggcaggctg gcctgattgg tttaagccgg gacactttat
      541 cagttttagc tggcttatta agtctggttg aaaaacacac tgcgctggcc cgggagaaaa
      601 atgttttgga agttttttat actgctttaa aagatttggg atatattcag tacctggaaa
      661 aagaagatca ggcggctgcc tggcagctta accgggaaag tattagctat ttaaatcagt
      721 ttattaaaca aattcagaat tttgaggctg ataatgacag taaatcagtc aaaatttttc
      781 tggaatacat ggattttgtg attgaatcag gcgacatcgg ccagttatcg cctgactttg
      841 aaattgggcc ggagacttta aaaattttaa cggttcacgc cgccaaaggg ctggagttca
      901 aatatgtttt tatcgctaat ctggttgacc gtcgttttcc gacgatagac aggaaagatc
      961 caattgaaat tcccgatgag ctggtcaaag aaattttgcc cggcggtgat ttccatctgg
     1021 aggaagagcg gcggctgttt tacgtggcca tgacccgggc caaagagcat ttgtatttta
     1081 gttcggccag tgattacggc ggcgccagga aaaagaagct ttcccgtttt ctgcaggagc
     1141 taaatgatca gggatttcca attaatcagg cggaagaagt ctccgccaga ggcggatccg
     1201 cctctggcgg aagcctaccc tctttcaggc ttttccagcc ggtgaccccg attgaaaagg
     1261 aagttaaaca gcaggcggaa attattccgg ataaattcag ctatactcag ctgaaaattt
     1321 ttcaaacctg cccctggcag taccgctatc agtttgtttt aaaaattccg acatttggca
     1381 aagccacctt tagttttggg aaaacaatcc acggtgcttt gcagaaattt tttcaaaata
     1441 ttgttgaagg taaaattgcc cggcagacta atttattcgg taaaaagtcc ggggtcggga
     1501 cagggaaatt accatcgtgg gatgatttgt taaagttata tgaagaaaac tggattgacg
     1561 agtggtacgc tgatcaggac caaaagaaaa aatataaaga agaggggaga gcgattttaa
     1621 aaaattttta cggcgaagtt gaaaaagaaa tgcccaatgt ggttgagctg gagaaagggt
     1681 ttaatattaa aattaatgat tacacaatca aggggcagat tgaccggatt gatttaaagg
     1741 ctgacggcgg cattgaaatc attgattata aaaccggcaa tattcctgac gccaaatatc
     1801 tggacaagga ccagctgttg atttaccagc tggcggcttc tgaaatattt ggcaaaaatg
     1861 tttccaatct gacttattat tatttaaaca aaaaaggcaa ccaaaaactg tcttttttgg
     1921 gggctgacaa ggatttggat aaaattaaag ccaaagtcag ccagataatt gaagacatta
     1981 aaactagcaa ttttaccccc gcgcctgata aaatgaaatg taagtattgc gattacaaag
     2041 atatttgcga gtaccgaata atctaaaatc cccccttatt ccatgctaca cattaacaaa
     2101 atattaaaaa attccattaa aaaatcaggc ttagaaccgc agattgaagc ttcgctggtg
     2161 ctggaagaag ctggtcagat tttatacgcg atatttggcg acaagattgt taatcaggcc
     2221 cgaccgcttt atgttaaaaa caaaatttta acgattgccg ttttaagctc cattgttagt
     2281 caggagctta aattgagaga aagtgaaata attgataaaa taaataaaaa gtttgaaaag
     2341 aatttagtag caagattgag gttcgcggca taaaatttat ccgaatttac aaattctatt
     2401 ctaattgact aattacaaat atttctgttg ccaatttttc taatttacaa attaataaac
     2461 caaaatttgt tgaattagtc attattagca acaaagatat tggttatttg tagattagaa
     2521 ttaaattcgt tgattagtat aacattatat gactttgaga caatatttaa cattaatgat
     2581 catctccacc cttatttttt gggcggcttt tctgatggtt ttattgaata ttgatcctgg
     2641 ccaaaccgga ttttttggtt ttttattctt ctacttaagt ctgtttttag cattgaccgg
     2701 gacgattgct ttggttggtt tattaatcag gtcgtatact ctgaaacatg aaatgatttt
     2761 tcggctggtc acccggtcgt ttcgacaggg gttatttttt tcagttttaa tcattgggac
     2821 tttgctgctg cagagccagc ggttgttaac ctggtggaac acgatatttt tggtgattgg
     2881 cctgacggcg ttggaatttt tctttgtttc agataaaaga cgccgggtcc ctttgcggga
     2941 aaattatttt gatcaggaag atattgtgta aattattaca taatgactct atcgatatca
     3001 ttgttggtta tcagcattta tttatattca aaaacgaatc taccaaaatc ttatcggttc
     3061 gtttttagta tttttgtttt tattgctttg attcttaatt tattttacgg aatcgttgac
     3121 tatttaacag gaaacggggt aacagaagct gccttatatc atttaagtta tggtttgaca
     3181 ggtgccggat attttgatta ctggaaaatg attattatta ctgtcagcgt tattttgatg
     3241 gctctagttt tatttttttg gctttttttt agaaatccag ttcctcagat
//
LOCUS       DASWHZ010000070         6640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_151862, whole genome shotgun sequence.
ACCESSION   DASWHZ010000070 DASWHZ010000000
VERSION     DASWHZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151862"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1510
                     /locus_tag="VGA49_01585"
     CDS             485..1510
                     /locus_tag="VGA49_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HEX9664493.1"
                     /translation="MTIKDQIKRFLAKGKKATSTEMAKKFGFSRQHISANLRELVDAG
                     EVIKFGSTRSAFYALPEFIDEVGPVSVKRRFRNQNIQEHQIFNDLIAAFPAFRTVSEN
                     VQSIIHYAFSEMLNNAIEHSSSGSIEIKMDSDEGIIRFVVEDYGIGVFRNVMRQRKLK
                     SEFEAMQDLLKGKVTTAPQAHSGEGIFFTSKAADRFVLESFGYKMVIDNTVPDVFFQE
                     ESKINRGTRVIFTIARGSQRHLNAIFKKYQSEPGSHAFDKTEIYVRLFTMGTIYVSRS
                     QARRILSGLEKFKTIILDFERVPNVGQAFADEIFRVFQNRYPDIAIESVNMNETVRFM
                     VERVEKR"
     gene            1530..2306
                     /locus_tag="VGA49_01590"
     CDS             1530..2306
                     /locus_tag="VGA49_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX9664494.1"
                     /translation="MSQYYTPHRSASWNYGGDKWKLSRSKIDLFLGCPRCFYLDNKLG
                     VKRPAGYPFNLNTAVDTLLKKEFDLHRVKGTQHILQKEYKIDAIPIPHEDLDAWRHNF
                     TGIQYLHPGTKMTISGAIDDLWQNSKEEYIVVDYKSTSINEDIKDIDKDWQASYKRQM
                     EVYQWLLRQKGYPVSDTGYFVYCNGRTDKKAFDKKLEFDVTLISYIGNADWVEGTIKD
                     IHECLNKKNIPRSDPECAYCQYRGAVEKVSKNKLWAGTLF"
     gene            2285..2860
                     /locus_tag="VGA49_01595"
     CDS             2285..2860
                     /locus_tag="VGA49_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664495.1"
                     /translation="MGWYTFLIISHLIGTALGVGGATASDFLFFKSIKDRQIDRTEFG
                     FLKIVSNLVWAGFLILVFSGFGFLLLYRLQLPALAPDSWQRFWAKMTVVMIIFLNGLV
                     MHWKVFPLFEEHIDKKLITPDFIKKAPIVFTTGAISIISWYSGLILGAWRELTLSYLE
                     IIFLYGVILAIGISVSNLIGRYFIRNSFSKK"
     gene            complement(2929..3195)
                     /locus_tag="VGA49_01600"
     CDS             complement(2929..3195)
                     /locus_tag="VGA49_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9664496.1"
                     /translation="MAKKLYVGNLPYDTTEEKLGELFEGAGSVESVNIITDKFSGRSK
                     GFGFVEMSSDEEAQKAIEMFNEKDFGGRNMTVNEARPMKDRNDR"
     gene            3379..4728
                     /locus_tag="VGA49_01605"
     CDS             3379..4728
                     /locus_tag="VGA49_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9664497.1"
                     /translation="MNNHNRLITERIPKLIKEIALPASVGFFFHTMYNVVDTFYGGLI
                     STEALAALTLSFPVFFIIIAIGSGISTGGTVLISNHIGAGRHDEKLVYSAQVISFGVL
                     ISFFLTVLGLATAPFLFKLLGASAQYLALSLAYINVIFYGTVFFLLNNVLNSALVAIG
                     DTKTFRNYLIIGFFLNLILDPWFLLGGFGLPAMGLAGIALATVVIQVLGCFYMYYKVV
                     KAGMFSRESWSLLKPRKKTFFEIAQQGFPASLNMMTVAIGIFVITYFISWFGQTAVAA
                     YGIATRVEQIALLPSIGLNIAALTLIGQNNGAKQYQRIKIIIRKCLKYGLIIMTFGAI
                     AVFFLPRQLMGIFTGDQAVILIGSQYLKIAAFILWAYVLIFINVSAMQGVKKPMFAVW
                     IGLYRQIIAPIIVFLFLIKVMGWGLFGVWWGVFIITWSAALIALFYSKKVINRLDVV"
     gene            4739..5110
                     /locus_tag="VGA49_01610"
     CDS             4739..5110
                     /locus_tag="VGA49_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664498.1"
                     /translation="MKQFSLLILRIGLAITFIWIGVLIFRQPEAWGGYLQPWAANLLP
                     IPIKEAMLGVAVLDIFIGILILSRPVAWLAALIGSFHLLTVLIGSGINDVTVRDIGLL
                     AGMLAIVADHFADSSKKDIMT"
     gene            5112..5450
                     /locus_tag="VGA49_01615"
     CDS             5112..5450
                     /locus_tag="VGA49_01615"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9664499.1"
                     /translation="MVKLTEQRCVPCEGGVPPLKPDKITEYLGQLTLDWEVTDGKKIK
                     HQFEFKDFKEAIAFVNQVANLAATEGHHPDIHIYYDKVKIVLWTHAIRGLFDNDFILA
                     AKIEEIATGH"
     gene            5507..5674
                     /locus_tag="VGA49_01620"
     CDS             5507..5674
                     /locus_tag="VGA49_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664500.1"
                     /translation="MHYICTGGCQGLSDQPGVCQTPDCPNEGHPLKACDCADGRHYGD
                     WDEKSEEKSND"
     gene            5729..5935
                     /locus_tag="VGA49_01625"
     CDS             5729..5935
                     /locus_tag="VGA49_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX9664501.1"
                     /translation="MKKLSTLDWVAVVLLVIGGLNWGLVGLFSFDLVAAIFGDMSVLS
                     RIIYVLVGLSALYVLAMANKLGKK"
     gene            6004..>6640
                     /locus_tag="VGA49_01630"
     CDS             6004..>6640
                     /locus_tag="VGA49_01630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX9664502.1"
                     /translation="MFPIRINKFLSEANYCSRREADRLISAKKVKINDQIASLGDKVE
                     KNDQVFVEGLEIRLTTAEKIYLVFHKPVGIICTSAKVKDNIIDYINYPERIYAVGRLD
                     VASSGLILLTNDGELANKILKAQSKIEKEYLVTVSRKLTRQFLENLRRGVVIDHYKTL
                     PAKVSRISDKIFSIAIVEGKNRQIRKMCQRFGYDVLKLKRIRIGSLNLNKLK"
BASE COUNT         1881 a         1271 c         1414 g         2074 t
ORIGIN      
        1 gttgttgttg tctgtacccg taatataata agtagtgaat tagagcaagt cgctgataaa
       61 tatattcctg ctgaaacttt agcgcaattt ttagtttata ataataaaaa cactccgcct
      121 aaaaaggcgg aggcgtgagt acaatcctag tatcgttcag gcgacggcaa tcttgcgatt
      181 acattaaaat tatagcatag aaaagaaaat tgtcaaaatg cttattttag cttaaaatag
      241 aagttatcaa caccataaat catgtcccaa tactacaccc cccaccgctc cgcctcttgg
      301 aactacggcg ggaaaaaccg gaaactaaac cggtcaaaga ttactccagg ttacaattac
      361 cagacaatct aaaattcgca ccttacaatt tactttacaa ttacatttag tcactttaca
      421 attcacttta caattatata tagccacttg acaattattg gaaaatagtc tatattgtct
      481 atatatgact attaaagatc agataaaaag gtttttagct aaggggaaaa aggcaacttc
      541 cacggaaatg gccaaaaagt ttggtttttc tcgtcaacat attagcgcta acctgcggga
      601 actggttgat gccggcgaag taatcaaatt tggttcgacc cgatcggctt tttatgcttt
      661 gcctgaattt attgacgaag tcgggccggt atcagtaaaa cgtcgcttta gaaaccaaaa
      721 tattcaagaa catcagattt ttaatgattt aatcgcggcc tttcccgctt tcagaactgt
      781 ttcggaaaat gttcaaagta ttatccatta tgctttttct gaaatgctta ataacgccat
      841 tgaacattca agttcaggca gtatcgagat caaaatggac agtgacgaag ggataattcg
      901 ttttgtcgtt gaagattatg gcattggtgt ctttagaaat gtcatgcgtc agcgtaaact
      961 taaatcagag tttgaagcaa tgcaggactt gttaaaagga aaagttacga cagctccgca
     1021 agctcatagc ggcgaaggaa tattttttac ttcaaaagca gctgatcgtt ttgtgctgga
     1081 gagttttggc tacaaaatgg ttattgataa taccgttcct gatgtttttt ttcaggaaga
     1141 atcaaaaatt aatcgcggga cccgggtaat tttcactatc gctcgcggca gccagcgtca
     1201 tctaaatgca attttcaaaa aatatcagtc agagccgggg agtcacgcct ttgacaagac
     1261 tgaaatttat gtcaggctat ttacaatggg gacaatctat gtttcccggt ctcaagcacg
     1321 gcgtatcttg tcaggacttg aaaaatttaa aacaatcatc cttgattttg aacgggtgcc
     1381 taatgtcggc caggcgtttg ctgatgaaat atttagagtg tttcagaatc gatacccgga
     1441 catcgctatt gaatcagtta atatgaacga aaccgtgcga tttatggttg agcgagttga
     1501 aaaacgataa ccaattaaca tcctaaatta tgagccaata ctacaccccc caccgctccg
     1561 cctcctggaa ctatggcggc gacaaatgga aacttagccg gtcaaaaatt gatctgtttt
     1621 tgggatgtcc cagatgtttt tatctggata ataagctggg cgttaagcgg ccggctggtt
     1681 atccatttaa tctgaacacc gccgttgata ctttgcttaa aaaagaattt gatctccacc
     1741 gggtcaaagg gacccagcat attttacaaa aagaatataa aatagacgct attcctattc
     1801 cccacgaaga tcttgacgcc tggcggcata attttaccgg cattcaatat ttgcatcccg
     1861 gcactaaaat gactatcagc ggagcgattg atgatttgtg gcaaaactca aaagaagagt
     1921 atattgttgt tgattataaa tcaacttcta ttaatgaaga tattaaggat atagacaagg
     1981 actggcaggc cagttataaa cgacaaatgg aagtttatca gtggctcttg cggcaaaaag
     2041 gctatcctgt gtcagacacc ggctattttg tttactgcaa cggccggaca gacaaaaaag
     2101 cttttgataa aaaactggag tttgatgtca ctttaatttc ttacatcggc aacgctgact
     2161 gggtggaagg gacaattaaa gatattcatg aatgtttaaa taaaaaaaat attccccggt
     2221 cagatccaga atgcgcttat tgccagtaca ggggagccgt tgaaaaagtt tctaaaaata
     2281 aactatgggc tggtacactt ttctaatcat ctcccacctt atcggcacgg cactgggcgt
     2341 tggcggagcg accgccagtg attttttgtt tttcaaatca attaaagacc ggcagattga
     2401 caggactgaa tttggttttt taaaaattgt ttccaatctg gtctgggctg gatttttgat
     2461 tttggttttt tccggttttg gttttttgct tttgtaccgg cttcaactgc cggccttggc
     2521 tcctgattcc tggcagagat tttgggctaa aatgaccgtc gtgatgatta tttttctgaa
     2581 cgggctggtg atgcactgga aagtttttcc tttgtttgaa gaacacattg ataaaaaact
     2641 gattacgcct gattttataa aaaaagcccc gattgttttc acgaccggcg ctatttctat
     2701 tatctcctgg tattcaggct taattttagg cgcctggcgg gaactgactt tgtcttattt
     2761 ggaaattatt ttcttgtacg gggttattct ggccattggc attagcgtct ctaatttaat
     2821 tggccggtat tttatccgaa atagtttttc aaaaaaataa aatacgtttt gtaaactaaa
     2881 accccgtcac tcgcgagcga cggggttttg ttatattttc aggaagaatt atcggtcgtt
     2941 tctgtctttc attggccggg cttcattgac ggtcatattt cgaccgccaa agtctttttc
     3001 gttgaacatt tcaatcgctt tttgagcttc ctcgtcagag gacatttcaa cgaagccaaa
     3061 acctttgctt ctgcctgaaa acttgtcggt aatgatgttg actgattcaa ctgatccggc
     3121 gccttcgaaa agttcgccca gcttttcttc agtcgtgtcg taaggtaagt taccgacgta
     3181 aagtttcttt gccataatag gtattatacg gtgcacgggg taaaaaatac tccaaaacgc
     3241 actttctaat tgttttaatt gttaatcttg tttcgaccgt acaccttgaa ataagatgct
     3301 aacaaggtca ttcaatatag cacatattac aaaattgcgc aagcggctat taaaggctat
     3361 aattattgaa ctgtttatat gaataatcac aatcgtttaa tcaccgaaag aattcccaaa
     3421 ctgatcaagg aaatcgctct gccggccagc gtcggttttt tctttcacac catgtacaat
     3481 gtcgttgaca ccttctacgg cggactgatt tccactgaag cgctggcggc tttaacttta
     3541 tcttttccgg tttttttcat catcatcgct attggttccg gaatctccac cggcgggaca
     3601 gttttgatct ccaatcacat tggcgccggc cggcacgatg aaaagctggt ttattcagct
     3661 caggtgattt cttttggcgt attaatttct ttctttttaa cggtcctggg gctggcgaca
     3721 gctccttttt tgtttaaact gctgggagcg tctgctcaat acctggcttt gtcgctggct
     3781 tacattaacg ttatttttta cgggacagtt ttctttttat tgaataatgt tttaaattca
     3841 gctttagtgg ccattggcga cactaaaact tttcgcaatt atttgattat tggttttttc
     3901 cttaatctaa ttttagaccc ctggttttta ttgggcggtt ttggtctgcc ggcgatgggg
     3961 ctggccggca ttgctttggc gacagttgtt attcaggttc tgggctgttt ttatatgtat
     4021 tacaaagtgg tcaaagccgg gatgttctcc agagaaagct ggagcctgct taaaccgcga
     4081 aaaaaaacat tctttgaaat cgcccagcag ggttttccgg ccagcctgaa tatgatgaca
     4141 gtggccatcg gaatttttgt gattacttat tttatctcct ggtttggcca gacggccgtg
     4201 gccgcttacg gtattgccac cagggtggag caaattgctt tattgccgtc aatcggactg
     4261 aatatagccg ctctaacttt aattggccag aataacggcg ctaaacaata tcagcggatc
     4321 aaaattatta tcagaaaatg tctgaaatac ggtctgatta ttatgacttt tggcgcgatt
     4381 gccgtctttt ttctgccgcg gcagttaatg gggattttca ccggggatca ggcggtgatt
     4441 ctgattggct ctcaatattt aaaaattgcc gcttttatcc tctgggctta tgttttgatt
     4501 tttattaatg tttccgccat gcagggagta aaaaagccga tgtttgctgt ttggatcggg
     4561 ttgtatcggc aaattattgc tccaataatt gtttttttat ttttaatcaa agtaatgggc
     4621 tggggcttat tcggcgtttg gtggggggtc tttattatta cctggtcagc cgctttaatt
     4681 gccttatttt attcaaaaaa ggttatcaac aggctggatg tagtataata aaagaattat
     4741 gaaacaattt tcattattga ttttacgtat cggcctggcg attactttta tctggattgg
     4801 cgttttgatt tttcgccagc ctgaagcctg gggcggttat ctccagccct gggcggccaa
     4861 cttattacca atcccaatca aagaagcaat gctgggagtg gctgttctgg atatttttat
     4921 tggcatatta attttatcca ggccagtcgc ctggctggcc gctttgattg gttcatttca
     4981 tttgctgact gttttgattg gttccggcat taacgacgtt actgtccggg atattggttt
     5041 actggccggc atgctggcaa tagtggctga tcattttgct gattcatcaa aaaaagatat
     5101 aatgacttaa tatggttaaa ttgactgaac aaaggtgcgt cccctgcgaa gggggagtgc
     5161 ctcctttaaa accggataag atcacagaat atttagggca gttgacgctg gactgggaag
     5221 taactgacgg caaaaagata aaacatcagt ttgaatttaa agattttaaa gaagcaatcg
     5281 cttttgtcaa tcaggtggcc aatttagctg caacagaagg tcaccatccc gatattcata
     5341 tttattacga caaagttaaa attgttctct ggacccacgc tatcaggggt ctttttgata
     5401 atgattttat tttagcggcc aagattgaag aaatagccac tggtcactag ttattagcca
     5461 ctagggttac tagtgaccaa tgactaatga ctaatgacaa aaaaatatgc attatatctg
     5521 caccggcggc tgccagggtt tgtccgatca gcccggcgtc tgccaaacac cagactgccc
     5581 caacgaaggt catcctttaa aagcctgcga ttgcgctgac ggccggcatt atggcgactg
     5641 ggacgaaaag tcagaagaaa aatctaatga ctaataataa attttaataa aggtcgatct
     5701 taattaacaa ttaaataaac ataaaactat gaaaaaattg agcacactcg actgggtcgc
     5761 cgtggttctt ttggtgattg gcggcttaaa ctggggcttg gttggcctgt tcagctttga
     5821 tttagtggcc gctatttttg gcgatatgtc agttttatcc agaattattt acgttttggt
     5881 tggtttgtcc gctctgtatg ttctggccat ggctaacaag ttaggtaaga aataattcgt
     5941 ttacaactga ataaacattc caggcagaga ttaacctgtc taatttttta gtattttaaa
     6001 ttaatgtttc ccatcagaat caataaattt ttaagcgaag ctaattactg ctccagacgg
     6061 gaagctgacc gtttaatctc tgccaaaaaa gttaaaatca acgaccaaat cgccagcctg
     6121 ggcgataaag ttgaaaaaaa tgaccaggtt tttgttgagg gcctggaaat cagattaacc
     6181 accgcggaaa aaatctacct ggtttttcac aaacctgtcg ggattatctg tacttcggcc
     6241 aaagtcaaag ataatattat agattatatc aattatccgg aaagaattta tgcggtcggc
     6301 cggctggacg tggcctcatc cggtctgata ttgctgacta acgacggaga actggctaat
     6361 aaaattttaa aagcccagag taaaattgaa aaagaatatt tagtgacagt cagccgcaag
     6421 ctgaccagac agtttttgga aaatttaaga aggggcgtgg tcattgatca ttataaaacc
     6481 ctgccggcca aagtcagtcg aatcagcgat aaaatttttt caatcgccat tgtggagggg
     6541 aaaaaccgtc agatcagaaa aatgtgtcaa agatttggct atgatgtttt aaaacttaaa
     6601 agaatcagga ttggctcttt aaatttgaat aaattaaagg
//
LOCUS       DASWHZ010000071         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_537420, whole genome shotgun sequence.
ACCESSION   DASWHZ010000071 DASWHZ010000000
VERSION     DASWHZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537420"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /gene="gltX"
                     /locus_tag="VGA49_01635"
     CDS             complement(<1..1350)
                     /gene="gltX"
                     /locus_tag="VGA49_01635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9664503.1"
                     /translation="MSEVRTRFAPSPTGFLHIGGLRTALYNYLFAKKNKGKFVLRIED
                     TDRERYVKGSEKQIINSLLWADLNYDEGPRINNQGQLEPGDNQFGPYIQSKRLELYAN
                     FAEDLIDKGLAYRCYCSKQRLEDLRKKQMAARLAPKYDRYCLNNKPSGKKYVIRFKIP
                     EEGTTGWRDLVHDKVKFDNKILEDFVMMKQDGLPTYNFANVIDDHLMKISDVIRGEEF
                     LSSTPKHLLLYRALGWPTPQFAHIPLILGKNKAKLSKRDGDVAVDDYFKKGYLPDALI
                     NFVALLGWNPGTDQEILDREALIYKFSLKQINKSNAIFDLDKLDWMNGCYIRQRNPED
                     LTREAVPFLIKAKFLKITQKNGYINPKNKEVIYFDDLKKIIRLEQGRMKKLSELPELI
                     DFVFVDKLKYDPSLLIWKKMTKKELREGLTIISQYLNTFPEKNFTAEKLDNKIKELIK
                     "
     gene            complement(1354..1908)
                     /locus_tag="VGA49_01640"
     CDS             complement(1354..1908)
                     /locus_tag="VGA49_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX9664504.1"
                     /translation="MEVIKIITTNKSKVFLLNQIFDKNKYKIRVEMTDYLYPEIKRTD
                     DQKELVRYAAKHCARKFSKKCLVTELGLYIKTLKEFPGIQTNYVLKRIGQDGIIKLLN
                     QQSDRQAEIRLALGYCVPGKDTKVFTAVVPGRLTREEKGQNDLGFESIFIPDGHQITL
                     GQDLGLRNELLSKIYKDFAQYYLN"
     gene            complement(1871..3085)
                     /locus_tag="VGA49_01645"
     CDS             complement(1871..3085)
                     /locus_tag="VGA49_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX9664505.1"
                     /translation="MYRSIILGVEIDKITRGDARQRFKDFIYSKNQHYIVTPNPEIII
                     AAQKDDDYKKILNQSSLSLPDGAGLYLAGLIFKDQIRERITGTDFVEDICQLAQKNNW
                     SLYLAGGAGQTARRAADNLRQQYPKLKIAAAEEGMTPENFSFDSQDIINKINRAGPQI
                     LLVAFGAPKQEKWIYYNLNKLKTVQVAMGVGGAFDYISGNVKRAPAWMRKMGLEWLFR
                     LLTQPWRLNRIWTATIVFCGRIIYWRLRMIFKYRQNVAALVFNKHNQILVISPRWKKY
                     LSWALPQGGIKKKEGAGQAARRELSEELGTDQKNFKVIKIFTNFYEYNWPKWARQLKG
                     YKGQKQALAIVKFSGSDKDLNIARSAEVKAIKWVTPENLLKVIEDKRRDMVQKALTKM
                     YGSHQDNHHQQE"
BASE COUNT          811 a          671 c          572 g         1041 t
ORIGIN      
        1 ttttattaac tctttaattt tattgtctaa tttttcggcc gtaaaatttt tttcagggaa
       61 agtatttaaa tattgagaaa tgatagtcag cccttcccgc agttcttttt tggtcatttt
      121 tttccaaatt aaaagactgg ggtcatattt aagtttatca acaaaaacga aatcaatcag
      181 ttcaggcagc tcgcttagtt ttttcatccg cccctgctcc agcctgataa tttttttcag
      241 gtcatcaaag taaatgactt ctttgttttt gggattaata taaccgtttt tttgagtgat
      301 tttcaggaac ttggctttaa ttaaaaatgg caccgcctcc ctggttaagt cttcaggatt
      361 tctttggcgg atatagcagc cgttcatcca gtctaatttg tccaagtcaa aaatggcgtt
      421 agacttgttg atttgtttta aggagaattt ataaatcaga gcctcacggt ctaaaatttc
      481 ctgatcagtc ccgggattcc agccaagcaa agccacgaaa ttaatcaaag cgtcaggcaa
      541 ataacctttt ttaaaataat catcaacggc cacgtcgccg tctcttttgc tcaatttagc
      601 tttattttta cctaatatca gggggatatg ggcaaattga ggagttggcc agcccagcgc
      661 ccggtaaagc agaagatgtt ttggagtgct tgataaaaat tcctctcctc tgataacatc
      721 tgatattttc attaaatgat catcaatcac gttggcaaaa ttataagttg gaaggccgtc
      781 ctgcttcatc attacaaagt cttccaggat tttattgtca aatttaactt tatcatgaac
      841 caaatcccgc cagccggtag tcccctcttc cggaatttta aaccgaataa catatttttt
      901 gcctgacggc ttattgttta agcaatatcg gtcgtatttt ggcgccagcc tggcggccat
      961 ttgttttttt cttaaatctt ccaaacgttg tttggaacag taacagcggt aagccaggcc
     1021 tttatcaatt aagtcctccg cgaagttcgc ataaagctcc agtctttttg actgaatata
     1081 aggaccaaac tgattatcgc ccggttccag ttggccctga ttgttaattc tgggaccttc
     1141 atcataattc agatcagccc ataatagact attgattatt tgcttttcag agcccttaac
     1201 gtatctttcc cggtctgtgt cttcaatcct taaaacaaat tttcctttat tctttttggc
     1261 aaacaaataa ttgtaaagag ccgttctcaa tcccccaata tgaagaaaac cggtcggcga
     1321 cggggcaaat ctggttctga cttctgacat aaattaattt aaataatatt gagcaaaatc
     1381 tttgtaaatt ttgctaagca gttcatttct taaacctaaa tcctggccca aagttatttg
     1441 atggccatca ggaataaaaa ttgattcaaa gcccaggtca ttttggcctt tctcttccct
     1501 ggtcagcctg cccggaacaa cagcagtaaa aacttttgtg tcttttccgg gaacgcagta
     1561 gcccagagcc agtctgattt ccgcctgccg gtcagactgc tggttgagca atttaattat
     1621 accgtcctgg ccgattcttt tcaaaacata attagtttga atgccgggga actcctttaa
     1681 ggttttaata taaagcccca gttcagttac caggcatttt ttgctgaatt ttctggcgca
     1741 atgcttggcc gcgtaccgaa cgagctcttt ttgatcatca gtccgtttga tttccggata
     1801 gagataatct gtcatttcaa ctctgatttt atatttgttt ttgtcaaaaa tttgattgag
     1861 taaaaaaact ttactcttgt tggtggtgat tatcttgatg acttccatac attttcgtta
     1921 atgctttttg aaccatgtcc ctccttttgt cttctatgac ctttaataaa ttttcaggcg
     1981 ttacccactt aatggcttta acttcagctg atctggctat atttaaatct ttatcagaac
     2041 cgctgaactt aacaatcgct aaagcttgtt tttggccttt ataacctttg agctgtcggg
     2101 cccattttgg ccagttatat tcataaaaat tggtaaatat tttaattact ttaaagtttt
     2161 tctgatcagt tcccagttct tcagataatt cacggcgggc cgcctgcccg gctccttcct
     2221 tttttttaat tcctccctgg ggcagagccc agctaaggta ttttttccag cggggcgaaa
     2281 tgaccaaaat ttgattgtgc ttgttaaaaa ccagagcagc cacattttgc cggtatttaa
     2341 aaatcattct aagccgccag taaattatcc ggccgcaaaa aactatggtg gcagtccaga
     2401 tcctattcag ccgccagggt tgagtcaata aacgaaacag ccactcaagc cccatttttc
     2461 tcatccaggc cggggctcgt ttgacattgc cggagatata gtcaaaagcc ccgccaaccc
     2521 ccatggccac ctgaactgtt ttaagtttgt ttaaattgta atagatccat ttttcctgtt
     2581 tgggcgctcc aaaagcaact aataaaattt gcggtccagc ccggttaatt ttattaatta
     2641 tatcctggct gtcaaagctg aaattttcag gcgtcatccc ctcttcagcg gcggcgattt
     2701 ttaattttgg gtattgttgt cgcagattat cagccgctct tcgggcggtc tgtccggcgc
     2761 cgcccgccag atacagcgac cagttatttt tttgagctaa ctggcagatg tcctcaacaa
     2821 aatcagtccc ggtgattctt tccctgatct ggtctttaaa tattaagccg gccaaataga
     2881 gaccagctcc gtcaggcagc gacaaggacg attggttcag aatctttttg tagtcatcat
     2941 ctttttgagc ggcaataata atttccggat taggggtgac tatataatgc tgattttttg
     3001 aatagataaa atctttaaat ctttggcggg catcccctct ggttatttta tctatctcta
     3061 cgcctaaaat tatcgatcgg tacatatatt ttttc
//
LOCUS       DASWHZ010000072         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_537508, whole genome shotgun sequence.
ACCESSION   DASWHZ010000072 DASWHZ010000000
VERSION     DASWHZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537508"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /gene="rny"
                     /locus_tag="VGA49_01650"
     CDS             <1..1073
                     /gene="rny"
                     /locus_tag="VGA49_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX9664506.1"
                     /translation="EREIKDELLQRINKIADENSEDLERKAKAVLASVIQRCATSHAA
                     EITTTTLDLPSDEMKGRVIGKEGRNIKTIEQLTGVEIIIDDTPQMLTISGFSPIRRHV
                     AKRALEKLILDGRINPARIEDAVLQAKKDMALDIKKAGEDALYEVGITGLDPKLVQII
                     GRLKYRTSYGQNILRHSIEVANLSALLAEELGANVAVAKKGGLLHDIGKAVDHELKGT
                     HTEIGRDIAKKFNIPEEVIYPIACHHDDRPPTLEAIIVKIADAISGARPGARKDTYEN
                     YIQRLTELEDLASEFSGVEKVYAIQAGREVRVFVTPDQIDDLAAVKLARSIANDIENK
                     LKYPGEIKVNVIREKRIVEYAR"
     gene            1110..1910
                     /locus_tag="VGA49_01655"
     CDS             1110..1910
                     /locus_tag="VGA49_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX9664507.1"
                     /translation="MIKIFFFGDVVAKIGRRAVKKIIPEIRKKYRPDLIMANAENIAH
                     GIGTTPKTLNELTEAGVDFFTNGNHIWKKEPVKEILETADSVMIRPANYPANRPGRGE
                     KIIEISQKKLLVINLLGRVFMEDQSVNNPFKTADQILEKYADENLAGIIIDFHAEATS
                     EKNAFGRYLDGKVSAILGTHTHIQSADEKILPGGAAYITDVGMTGAENSIIGVKTETV
                     INNFVNEVQDYFDYDETGPAVVNGVYLEIDSKTKKAVTIKRFQKKIII"
     gene            1924..>2220
                     /locus_tag="VGA49_01660"
     CDS             1924..>2220
                     /locus_tag="VGA49_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9664508.1"
                     /translation="MNKAELIESIADKIGAQKKQVEDVIEAFQTVVKNTLKQGNEVTL
                     AGFGTFSAKFRASRMGVNPQNPTEKIEIPAVTIPKFKAGKNLKDALKEKIPTETP"
BASE COUNT          688 a          563 c          505 g          464 t
ORIGIN      
        1 ctgaacggga aattaaagac gaattgcttc aaagaattaa caaaatcgct gatgaaaaca
       61 gcgaggatct ggaaagaaaa gccaaagctg ttctggccag cgtcattcag cgctgcgcca
      121 cttctcacgc cgctgaaatt accaccacca ccctggacct gccgtcagat gaaatgaaag
      181 gccgggtgat tggcaaggaa ggccggaata ttaaaactat tgaacagctg accggcgtcg
      241 aaatcattat tgacgacacc ccccagatgc tgactatctc cggcttcagt ccgattcggc
      301 gccatgtcgc caaacgagcc ctggaaaaac tgattctgga cggccggatc aacccggccc
      361 ggattgaaga cgccgtcctc caggccaaaa aagacatggc tctggatatt aaaaaagccg
      421 gcgaagacgc cctctatgag gtcggcatta ccggtctgga ccccaagctg gttcagatta
      481 tcggccggct gaaataccgg acatcttacg gccagaatat tctccgccac tcaattgaag
      541 tcgctaatct gtcagccctg ctggccgaag aactcggggc caacgtggct gtcgccaaaa
      601 aaggcggcct gctccacgac attggcaagg cggttgacca tgaactgaaa ggcacccaca
      661 ctgaaatcgg ccgtgatatc gccaaaaaat ttaatatccc cgaagaagtc atttatccga
      721 tcgcctgcca tcacgacgac cggccgccga ctctggaagc gattattgtc aaaatcgccg
      781 acgccatttc cggcgcccgg cccggagccc ggaaagacac ttacgaaaac tacatccagc
      841 gcctgactga actggaagac ctggccagtg aattcagcgg ggttgaaaaa gtctacgcca
      901 tccaggccgg ccgtgaagtc cgggtctttg tcactccgga tcaaattgat gatctcgcgg
      961 cagttaaact ggcccggtca atcgccaatg acattgagaa caaactgaaa taccccggtg
     1021 aaatcaaagt taatgtcatc cgtgaaaaaa gaatcgttga atacgctaga taattaaacc
     1081 gtcccgccga tcctggcggg acgcttcgta tgataaaaat cttttttttc ggcgatgttg
     1141 tcgccaaaat cggccggcgg gccgtcaaaa agattatccc ggaaatcaga aaaaaatacc
     1201 ggcctgattt aatcatggcc aacgctgaaa atatcgctca cggcatcggg acaactccca
     1261 aaactcttaa tgagctaacc gaggccggcg ttgatttttt taccaacggc aaccatatct
     1321 ggaaaaaaga accagtcaaa gaaattctgg aaaccgctga ttcagtcatg atccggccag
     1381 ccaattatcc ggctaaccgg ccgggccggg gtgaaaaaat tattgaaatt agccaaaaaa
     1441 aattgcttgt gataaactta ctgggcaggg tctttatgga agaccagtcc gtgaataatc
     1501 ctttcaaaac cgctgaccaa atcctcgaaa aatacgctga cgaaaatctg gccggaataa
     1561 taattgattt tcacgccgag gccacttccg aaaaaaacgc ttttggccgg tatctggacg
     1621 ggaaagtcag cgcgattctt ggaactcaca cccatattca gtcagctgat gaaaaaatcc
     1681 tgccgggcgg cgcggcctac atcactgatg ttgggatgac cggagctgaa aattcaatta
     1741 tcggcgtcaa aacggaaaca gtcattaata attttgtcaa cgaggtccag gattactttg
     1801 attatgatga aaccggcccg gcagtcgtca atggagtcta tctggaaatt gattccaaaa
     1861 caaaaaaagc tgttaccatc aaaagatttc aaaaaaaaat tataatctaa aatatttaat
     1921 acaatgaaca aagctgaact cattgaatca attgccgaca aaattggcgc tcaaaaaaag
     1981 caggttgagg atgtgattga ggcttttcag acagttgtta aaaatactct gaagcagggc
     2041 aatgaagtca ccctggccgg ctttgggact ttttccgcca aattccgggc ttcccggatg
     2101 ggcgtcaacc cacaaaaccc gacagaaaaa attgaaattc cggctgtaac cattcccaaa
     2161 ttcaaagccg gcaaaaacct caaagacgcc ctcaaagaaa aaatacccac agaaactccg
//
LOCUS       DASWHZ010000073         7753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_153119, whole genome shotgun sequence.
ACCESSION   DASWHZ010000073 DASWHZ010000000
VERSION     DASWHZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153119"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VGA49_01665"
     CDS             complement(<1..524)
                     /locus_tag="VGA49_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664509.1"
                     /translation="MTISERKLIFQLSLFIAVAVFLILGFVLSPFYLSLGLGLIIIFS
                     LLAVRLEWGIYLIAALFPFINLQIFIGKSFNVPYVDLVAMAVFAAWLIKLFWDWANTG
                     RFIKLKDLPGIFFFLLFIGASLLSLYNSQNVLQSLKFVLRPLAFFYLMFVIVPYNIIK
                     DFKALKNIIRIMFGV"
     gene            complement(565..1560)
                     /locus_tag="VGA49_01670"
     CDS             complement(565..1560)
                     /locus_tag="VGA49_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9664510.1"
                     /translation="MTNKINHNLIDAVIGTTYICNSRCIMCNIWQIKDQPTLTLADYR
                     KLPKTLKYINISGGEAFMHPEIVEIVRVTAETCHQAKLTISTNGFSTDLILDRMKQIL
                     EFAPEIGVSISLDGIGEKQYEVRRIPDGYEKIMRTFKGLQKLGIANSRLRLAFTAGDY
                     NIEHLSKVYDLTRELGIEMTLAAVHNSDNYFMIATNKITRFDEFKKEFDYVIKQELKT
                     FKPKRWLRAYFTYGLYKFVTTGKRILPYMAGRNSFYLDPDGWVFSTTVATKRMGNLKE
                     FNSLEDLMDASQAQEVLANDTEGHNWMICTTRAAMKSHPFRVGFWILKNKFLGMR"
     gene            complement(1843..3294)
                     /locus_tag="VGA49_01675"
     CDS             complement(1843..3294)
                     /locus_tag="VGA49_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9664511.1"
                     /translation="MKKLFILLSLMALSAVVGCKDKDRQPFPFPMETESGQMVATVQI
                     VGPNTVNVDETIQLLALGDGHVHLTTGDWSVDNDQLAVVLADGRVVGLKTGSVEITYT
                     FGSLTESQTITVKGAAKQAASLTISPAAVTLEETDLVNFRVLANNTDGSTSDVTYRAT
                     YASDNEEFFTVDRWGQGVARKMGNAQLTVELTNPDNSKVTRMVSVVVIKLQKTLDDLM
                     ILAAGDRLDADDDVSLEVLAGFDDGSSAFIYDVSWNFDSTLVDISPMNVMSVKGNGTF
                     VLTAARDGKISQDVTVNTTVPPQKLTLSVAANDPLGVNGTVQLGLTNSQPISTLDWVI
                     TPSTAGSVNSSKVFKALEAGRVRVKARTDGGNPAESNEIEIVVLGNRGIIAGMYYCKK
                     AEADQVFGNQAGETYVISNQNGWSPQQVVVQSVSGQDYYILQATIAAGTDVQWNLQRG
                     NPGLSWYPNDTTASAPDVVDNGVGGKKFVHTAQ"
     gene            complement(3730..5049)
                     /locus_tag="VGA49_01680"
     CDS             complement(3730..5049)
                     /locus_tag="VGA49_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX9664512.1"
                     /translation="MRQIFILTLAILISLTPLAPASAYELTGLDLETVVRVDSLPDAF
                     HPADQYFYNPGNFEPADQVIQYGTGFFIDYRGCLLTNAHVVTDFDDSRFKNLQIRLST
                     DLALKPQPAYPADVLFYDQGIDLAVLCVNDPERNFHHFYDIADDSRIDLLKIKDDISI
                     LGYPDIGGDSITYSTGKITGFWKEADLNYFLGENYFEDKRLEVIKSDTITGPGGSGSP
                     VFDDDGNVIGMIFAAAFVPSGINFIISGKVLNDWYDLYINTASPSTSCLRDTEKNLFR
                     KEGVYYYDNFCIVQQNLNIENAVQLNYQERCGANLDDRRLKMAAYHIATGSTINKWWD
                     YLGKFCPVNQQTAVEQSSFAYAQTRAPLVVEQTKAIELKNGLEKSVGQLNLSSDNWYK
                     LVNAYIYGGYPIEAISQAVKLGGYTVHPEIPFGAWKSSQDYIKNIIK"
     gene            complement(5141..7357)
                     /gene="pnp"
                     /locus_tag="VGA49_01685"
     CDS             complement(5141..7357)
                     /gene="pnp"
                     /locus_tag="VGA49_01685"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9664513.1"
                     /translation="MDQIKTFELDWGGRKLQVQTGDLAQQANGSCRVQYGETVVLATV
                     CASDQPREGISYFPLLVDYEEKFYAAGMIKGSRFIKRETRPSDEAVLTARMIDRTIRP
                     LFDGTTRNDIQVVVTTLSFDGENDPDIPALLGASIALSISDIPWDGPVAGIRIGQING
                     EWVINPSYEARQKSLIDIVAAGDGQKTIMLEAGAKEAGEEVVYQAIEFGQKHIGQVVK
                     FILDIQKEIGVTKEKPEVELTEEEQAAEKVKQEVETLTKEFVQAKVKEYLFADPLASK
                     LTRKSTLEEMKEELDDFLKEKQIGKEKRKYGQDFFYPCAEEVVSRAILETGQRVDGRA
                     LDEVRPLTIKAGVLPRTHGSCVFQRGETQIMALVTLGSPGMEQVMETIEESGKKRFMH
                     HYNFPGYSVGEVSPFRGASRRDIGHGALAEKALSSLVPEKEGFPYTIRVVSEVLGSNG
                     SSSMASTCASSLALMDAGVPFAKQVAGIAMGLVSDEQGNYKILTDLQDLEDTKGGMDF
                     KIAGTADGITAIQMDTKTKGLTLEMVKDTLEQAQKARLFILDEMNKVLSQPRPELSPY
                     APRIISFYINVDKIRDVIGPGGKMINEIIDKTGVEIDIEQDGLVMVTSVDEESAKKAV
                     DWIKEITREVEVGEEFEGKVVRIMDFGAFVEILPGQDGLVHISELAHYRVDKVTDVVK
                     LGDVVKVKVVGIDEQGRINLSMKALTEAPAGRDDFRRGNDRTEKRPFRKKKFFGRN"
     gene            complement(7488..>7753)
                     /locus_tag="VGA49_01690"
     CDS             complement(7488..>7753)
                     /locus_tag="VGA49_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664514.1"
                     /translation="LGYSRVGQPLKLSTLEYAAYASQAEIKEIIKILEKELDQRKKIS
                     QQYEKKWQAFVKKQKQSFKKNLDKLVTAKESAQIGELKKKLAG"
BASE COUNT         2050 a         1961 c         1659 g         2083 t
ORIGIN      
        1 acaccgaaca ttatccggat aatatttttg agagccttaa aatctttaat aatattataa
       61 gggactatga cgaacatcag atagaaaaaa gccagcggcc gcaggacgaa tttcaaactt
      121 tgtaaaacat tctggctgtt ataaagagac agtaatgaag ccccgatgaa cagcaaaaag
      181 aaaaatatcc cgggcaggtc ttttagttta ataaaccgac cggtattggc ccagtcccag
      241 aaaagtttaa tcagccaggc ggcaaaaacc gccatggcca ccagatcaac ataaggcacg
      301 ttaaaacttt tgccaataaa aatttgcaga ttaataaaag gaaacaaggc ggctatcaga
      361 taaatccccc actccagacg gaccgccaat agactgaaaa ttataattaa acccagcccc
      421 agacttaaat agaacggact caaaacaaaa cccaaaatta aaaaaacagc caccgcgata
      481 aacaggctta gctgaaaaat tagttttctt tcagaaatag tcatacctta actaaagtat
      541 aacataatat aattaaattt atttttatct cattcccaaa aatttatttt taagaatcca
      601 gaaaccaacc ctgaaagggt ggcttttcat ggccgcccgg gtggtgcaaa tcatccagtt
      661 atggccttcg gtgtcattag ctaaaacttc ctgggcctgg ctggcgtcca tcaggtcttc
      721 cagactgtta aactctttta agttgcccat tcttttagtg gcgacagtcg tcgaaaaaac
      781 ccagccgtcc ggatccaggt aaaatgaatt gcgaccggcc atgtagggca ggattctttt
      841 accggtcgtc acgaatttat acaacccgta agtaaaataa gctcttaacc agcgttttgg
      901 tttaaaagtt ttcagttctt gtttaataac ataatcaaac tcctttttga attcatcaaa
      961 acgggtgatc ttgttggtgg cgatcataaa gtagttgtca gaattatgaa cggccgccag
     1021 agtcatttca attcccagct cccgggtcag atcataaact ttggacagat gttcaatgtt
     1081 gtagtcaccg gccgtaaaag ccagtcttaa gcggctgttg gcaataccca acttttgaag
     1141 acctttaaaa gtccgcataa ttttttcata gccgtcagga atccgccgaa cttcatattg
     1201 tttttcgcca atgccgtcca gagaaatact gacgccgatc tccggcgcga attccaaaat
     1261 ttgtttcatc cggtcaagaa ttaaatcagt tgaaaacccg ttggtggaaa tagtcagttt
     1321 ggcttggtgg caagtttcag cagtcacccg gacaatctca acaatttctg gatgcataaa
     1381 agcttccccg ccgctgatgt taatgtattt taatgttttg ggcagtttgc gataatcggc
     1441 caatgtcaaa gtcggctggt ctttgatctg ccagatgtta cacataatac agcgggagtt
     1501 gcaaatataa gtcgtcccga tgacggcgtc aattaagttg tgattaatct tgtttgtcat
     1561 aatgttaaag tctttttata tcctgcaact gtagcacaaa ttattgattt agtcaacaaa
     1621 aaacagggat tatcaaggat tatcaagggg aatcaaggga aataaaggga aattaggcaa
     1681 gtgatccttg atatgccctg ctatcccctg ataatccttg ctaatccttg ctccctgatt
     1741 tcccctgcta tcccctgata atccctgata tcccttttta aaaaaaatgg agcaagctgt
     1801 aagactcact ccataaatgt tttctcctgt aaaatccgcc cgctattggg cggtgtggac
     1861 gaactttttg ccgccaacgc cgttgtccac gacgtcgggg gctgaagctg tggtatcgtt
     1921 gggataccac gacagtccag ggttcccccg ctggaggttc cactggacgt ccgtcccggc
     1981 ggctatggtc gcctgcagga tatagtaatc ctgcccgctg accgactgga caaccacctg
     2041 ctggggactc caaccgttct ggttggagat gacatacgtc tccccggcct ggttgccgaa
     2101 aacctggtca gcctcggcct ttttacaata atacatcccg gcgatgatcc cccggttgcc
     2161 cagaacaacg atttcaattt cgttgctctc ggccgggttg ccgccatcgg tccgggcttt
     2221 gaccctgacc cggcccgcct ccaaagcctt gaaaaccttg gaggaattaa ccgaaccggc
     2281 cgtggacggg gtaatgaccc agtccaacgt gctgatcggc tgactgttgg tcaatcccaa
     2341 ctggaccgtg ccgttgaccc caagcgggtc gttggccgcc accgacagag tcagcttttg
     2401 gggcggaaca gtcgtgttga ctgtcacgtc ctgggaaatc ttcccatcgc gcgccgctgt
     2461 caaaacaaaa gttccgttcc ccttgaccga cataacgttc atcgggctga tgtcaaccaa
     2521 agtggaatcg aaattccagc tgacatcgta gatgaacgcc gacgaaccgt cgtcaaagcc
     2581 ggccaggact tccaggctga catcgtcatc ggcgtccagc cggtcacccg ccgccaaaat
     2641 catcaagtca tcaagcgttt tctggagctt gatgacgacg acactgacca tccgggtcac
     2701 cttggagtta tccggattgg tcagctcaac cgtcagttga gcgtttccca tcttccgggc
     2761 aacgccctgg ccccagcggt caacggtaaa aaattcttcg ttgtcgctgg cgtaggtcgc
     2821 ccgataagtc acgtccgaag ttgacccatc ggtattgttg gccagcaccc tgaaattgac
     2881 caggtcggtc tcttccagag tgactgcagc cggcgagata gtcagactag ccgcctgctt
     2941 ggccgcgccc ttgaccgtaa tcgtctggct ctccgtcaga ctgccgaaag tataagttat
     3001 ctcaacactc ccggttttta acccgacgac ccggccatcg gccaaaacca cggccagttg
     3061 atcgttgtca accgaccagt cgccagtcgt caggtgaacg tgtccgtccc ccagcgccag
     3121 caactgaatg gtctcgtcaa cgttgaccgt gttggggcca acgatctgga ccgtggccac
     3181 catctgaccg ctttccgtct ccatcggaaa tgggaacggc tgacggtcct tgtccttgca
     3241 gccgaccacg gccgacagag ccatcaggct caacaaaatg aacagtttct tcatgaaagc
     3301 acctcccggg gcttgggttt cacgtggaac agaatggaat atcttagcaa attagagggg
     3361 aaatgtcaac acaagcaggg gagatcaggg gagataaggt cccgatcccg cctatggcgg
     3421 gatcgtggat cctcgatttt tctatcccga cctttggtcg aggatgaaaa atcgggagtt
     3481 gatgaaggaa gataagaggt aataacggat aataaaaacc gggctgaaaa gcccgcattc
     3541 attaatttct aattcctaat ttctaattac taaacaatat ctaaatatca aatttcaatt
     3601 tttcaaacga tttgggataa aaaaatttag aatttaaaac attatgattt ataatttgtt
     3661 tagtatttcg atattagata ttaggattta tatcaacaga tactattgga tcaattagaa
     3721 agtagaaatt tattttatta tgttcttaat ataatcctga ctgctcttcc aagccccaaa
     3781 cggaatctcc ggatggacgg tgtagccgcc taatttaaca gcttgagaaa tagcttcaat
     3841 tggatagccg ccgtaaatat aagcgttgac cagcttatac cagttgtcag aagataaatt
     3901 taactgacca actgattttt ccagtccatt ttttaattca atcgctttgg tttgctcaac
     3961 gaccagcggc gcccgggttt gagcgtaagc aaatgatgac tgctcaactg ccgtctgttg
     4021 attaaccgga caaaattttc ccagatagtc ccaccacttg ttgatcgtac tgccggtggc
     4081 aatgtggtaa gcggccattt tcagccgacg atcatccagg tttgctccgc atctctcctg
     4141 ataatttaac tgaacggcgt tttcaatatt taaattctgc tgaacgatgc aaaaattatc
     4201 ataataatat acgccttctt ttctgaaaag atttttttca gtatcccgca aacagctggt
     4261 ggatgggctg gcggtgttaa tgtacagatc ataccagtca ttcagcacct tgcccgagat
     4321 aataaaattt atcccgctgg gcacgaaagc ggccgcgaaa atcatcccaa tcacattgcc
     4381 gtcatcatca aagacgggcg aacccgaacc gcccggcccg gtaatggtgt cgcttttaat
     4441 cacttccagt cttttatcct caaaatagtt ttctcccaaa aagtaattta aatcggcctc
     4501 tttccaaaaa ccggtaattt tgccggttga ataggtgatg gaatccccgc caatgtcagg
     4561 ataccccagg attgaaatat catctttgat ttttaacaaa tcaatccggg aatcatccgc
     4621 gatgtcataa aaatgatgga aatttctttc cggatcatta acgcataaaa ccgccagatc
     4681 aatcccctgg tcataaaaca aaacatcggc cggataagcc ggttgaggct ttaaagccag
     4741 gtctgttgac agcctgatct gcagattttt gaagcggctg tcgtcaaaat cagtcacgac
     4801 atgggcgttg gtcagcaaac agccccgata atcaataaaa aaaccagtgc catactgaat
     4861 gacctggtct gccggttcaa aattacccgg attataaaaa tattgatcag ccgggtggaa
     4921 agcgtccggc agagagtcca cccgaacgac tgtttccaaa tccagaccgg tcagctcgta
     4981 ggctgaagct ggcgccagcg gcgttaagct aattaaaatt gccaaggtaa gaatgaagat
     5041 ttgacgcata aagatttatg tttgtttctg catagccggg atcagactcc ggataaatcc
     5101 gcagtctaat cccggctcgc ggtcggatac cggctgacta ttaatttctt ccgaaaaact
     5161 ttttcttccg gaacggccgc ttttccgttc ggtcatttcc ccgacggaaa tcatcccgac
     5221 cggccggcgc ttcagtcaga gctttcattg acagattgat ccggccctgc tcgtctatgc
     5281 cgacaacttt aactttaacg acatcgccca gtttaacgac atcagtcact ttatccaccc
     5341 ggtaatgagc cagttccgaa atatgaacca ggccatcctg ccccggtaaa atttccacga
     5401 aagctccaaa gtccataatt ctgacgactt tgccctcaaa ctcttcaccg acttcaacct
     5461 cccgggtaat ctctttaatc cagtcaaccg cttttttggc tgactcttcg tcaactgaag
     5521 tgaccatcac cagcccgtcc tgctcaatat caatttccac cccggtcttg tcaataattt
     5581 cattgatcat tttaccgccg ggtccgatga catctctaat tttatcaaca ttaatataaa
     5641 atgaaataat tctgggagcg tagggcgaca gttccggccg gggctgactc aaaactttat
     5701 tcatttcatc cagaataaac agccgggctt tttgagcctg ctccagagtg tctttgacca
     5761 tttccaaagt taggcctttg gttttggtgt ccatctgaat ggccgtaata ccgtcggccg
     5821 tgccggctat cttgaaatcc atgccgcctt tggtgtcttc caaatcctgc agatcagtca
     5881 aaattttata attcccctgt tcatcgctga ccagccccat ggcaatcccg gccacctgct
     5941 tagcaaaggg cacgccggcg tccattaagg ccagggatga agcgcaggtt gaggccattg
     6001 aactggagcc gtttgagccc aggacttcgg aaacaactct gattgtatag ggaaagcctt
     6061 ctttctccgg aactaaagat gacaaggctt tttcggccaa agcgccgtgg ccaatatccc
     6121 ggcgagaagc gcctctaaaa ggagaaactt cgccgactga atatcccggg aaattgtaat
     6181 gatgcataaa cctttttttg ccgctttctt caatcgtttc catgacctgt tccattcccg
     6241 gcgaccccaa agtaaccagc gccataatct gggtctcgcc ccgctgaaaa acgcaggaac
     6301 catgagtccg gggcaaaacg ccggctttga tcgttaaggg acgaacttca tccagggccc
     6361 ggccgtcaac ccgctggccg gtttccaaaa tcgcccggct gacgacttct tcagcgcagg
     6421 gataaaaaaa gtcctgtcca tattttcttt tttctttgcc aatctgtttt tctttcaaaa
     6481 aatcgtccaa ctcttctttc atttcttcca aagtgctctt ccgcgtcagt ttgctggcca
     6541 aaggatcggc aaataaatat tctttgactt tggcctgaac aaattctttg gttaaagttt
     6601 caacttcctg tttaactttt tcagccgcct gttcttcttc agttaattcc acctctggtt
     6661 tttctttggt gactccaatt tctttctgaa tatctaaaat aaacttaaca acctggccaa
     6721 tatgtttttg cccaaattca atcgcctgat aaaccacctc ttcgccggct tctttagcgc
     6781 cggcctccag cataattgtt ttctggccgt cgccggccgc cacgatgtca atcaagcttt
     6841 tttggcgggc ttcatagctg gggttgatga cccactcgcc gttaatctgg ccaattctga
     6901 ttccggcgac cggtccgtcc cagggaatat cagaaattga cagagcaatt gacgccccca
     6961 gcaaagccgg aatatcggga tcattttcac cgtcaaatga cagagtggtt accaccacct
     7021 gaatgtcatt gcgggtcgtg ccgtcaaaca acggccgaat agtccggtca atcatccggg
     7081 cggttaaaac cgcctcgtct gacggccggg tttcccgttt aatgaaacga gaccctttga
     7141 tcatgccggc ggcgtaaaat ttttcttcgt aatcaaccaa aagcggaaag tagctgatgc
     7201 cttcccgggg ctgatcgctg gcgcagactg tcgccaggac gactgtttcg ccatactgaa
     7261 cccggcagga gccgttggcc tgctgggcca ggtcgccggt ttggacctga agtttcctgc
     7321 ctccccagtc cagctcaaaa gttttaatct gatccataaa attcctttcg gatctgacca
     7381 tcagacttta gactatacgc ttaacctgat agtctacctg tctaacggcc agagattagt
     7441 taatggtagt tggtaattgg ttatattaat gaatgattgc tctaaagtca acctgccagc
     7501 tttttcttca attccccgat ctgagctgat tctttagccg taaccagctt atcaagattt
     7561 tttttaaacg actgtttttg ttttttgacg aaggcctgcc attttttttc gtattgctgg
     7621 ctaatttttt tcctttgatc cagttccttt tccagaattt taataatttc tttaatttcc
     7681 gcctgactgg cataggcggc gtattctaaa gttgacagtt tcaaaggctg tccaacgcgg
     7741 gaatatccca aaa
//
LOCUS       DASWHZ010000074         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1654589, whole genome shotgun sequence.
ACCESSION   DASWHZ010000074 DASWHZ010000000
VERSION     DASWHZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654589"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VGA49_01695"
     CDS             <1..1447
                     /locus_tag="VGA49_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX9664515.1"
                     /translation="MFKPDYHTLKNSQWYRQAGAIGLYYIDAACRGITKDIGPHWLFS
                     VKKEYGQNGFDINVLVRLGRQIIDKQLKDPKFLRNKFKNWTNFKKKVYQLAKILDKTD
                     LKSIADNKLIKLQKDFAKLNQQLWSLPILIDIFDYEGEKVVEEYLARYPELKLTSEEI
                     RDLLTPNEMTFGQQERLGLLNLVLKINKNNRIEKYTREIQQHAKKYFFYKNDWSKVWE
                     PDEDYFAKKINDELRNLKKAKREIKEIRDYQKNLIKRKQKIYKTKKIPQELKIIFKFF
                     ELLTFWRDVRKTDVQLINHYYYQFIKEFNHRTGIEEKYLLYLEPSAITSVKFIKDSLP
                     ELKRRRSGCVYVFNEKEKYKWVSGFGALKVIKLMDELIKKQSAELKGNTAYPGLVRGK
                     AKVIKTTADFKKMKKGDILVAPMTRPEYLPLMKIASAIITDEGGITCHAAIVSRELKV
                     PCVIGAQTATDVLKDDDRVEVDAGKGIIKKL"
     gene            1444..1857
                     /locus_tag="VGA49_01700"
     CDS             1444..1857
                     /locus_tag="VGA49_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664516.1"
                     /translation="MKNKILKIILIIVITLAVLIYLIFSFVNKRNTIKDVLNGQYQIV
                     SMITDGQSEIIDGDEIINFKKDGSWERSGVGGNWRGNYFIQDGQIVFSDYEDDQGQKQ
                     DQSLQGQLQLDGKDLIITYGQQAFWTQRIVVYEKL"
     gene            1920..>2624
                     /locus_tag="VGA49_01705"
     CDS             1920..>2624
                     /locus_tag="VGA49_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664517.1"
                     /translation="MKYLSYMLFGFLAFSLLFGANLAGAQTAATTTSASAEAQVNLEA
                     ELPKVRVNPVSVWYRVKLAWERVVDVFVSGEAQIRRHLLLTKKRLAEARASVNTRAEA
                     QISAVVERSRRRSEAALELYESIKVRLDSEARARVLAELNATARVQAEVIAEIYGEVS
                     AETRAGLDALIDVSNRSLQRLENSTEELKMELRSDDSAEAEIKARLEAFPVIEFREGS
                     TSEIDSTQESDRTEEES"
BASE COUNT          875 a          442 c          595 g          712 t
ORIGIN      
        1 gatgttcaag cctgattatc atacattaaa aaatagccaa tggtaccggc aggccggagc
       61 gattggactt tattatattg atgccgcctg caggggaatt acgaaagata tcggtcctca
      121 ctggttgttt tccgtcaaaa aggagtatgg acagaatggt tttgatatca atgttttagt
      181 caggctgggc cgccagatta ttgataaaca gcttaaagac ccaaaatttt taagaaataa
      241 atttaaaaac tggacaaact ttaagaaaaa ggtgtatcag cttgccaaaa tattggataa
      301 aactgattta aagtcaattg ccgataataa attgattaaa cttcagaaag attttgctaa
      361 attgaatcaa cagctttggt cactcccgat tttgattgat atctttgatt acgagggcga
      421 aaaggtcgtt gaggagtatt tggctcgtta tccggaatta aaattaactt ctgaagagat
      481 cagggactta ttaacaccaa atgaaatgac ttttggccag caggagagac tgggtttatt
      541 aaatttagtt ttaaagatta ataagaataa cagaattgaa aaatatacca gggaaattca
      601 acagcacgct aaaaaatatt ttttttataa aaacgactgg tctaaagtct gggagccgga
      661 tgaagattat tttgctaaaa agattaacga tgagttaagg aatttgaaaa aagctaaacg
      721 ggaaattaaa gagatcagag actatcaaaa aaatttaatt aaaagaaaac aaaaaattta
      781 caaaacaaaa aagattcccc aagagcttaa aattattttt aaattttttg aattattaac
      841 gttttggcgg gatgtccgga aaactgatgt tcagcttatc aaccattatt attaccagtt
      901 cattaaagaa tttaaccacc ggacggggat tgaagaaaaa tatttgttat atctggagcc
      961 atcagccata actagcgtta aatttatcaa agacagtttg ccggaattaa agcgccggcg
     1021 aagcggctgt gtctatgttt ttaatgaaaa agagaaatac aaatgggtta gcgggtttgg
     1081 agctttgaaa gttattaagt taatggatga gttgattaag aagcagtctg ctgaattaaa
     1141 agggaatacc gcttatccgg gtttagttag gggaaaagct aaagttatta aaacaactgc
     1201 tgacttcaaa aaaatgaaaa aaggagatat tctggtggcg ccaatgaccc ggccggaata
     1261 tctgccctta atgaaaattg cttcagcgat tatcactgac gaaggcggaa ttacctgcca
     1321 cgccgccatc gtctcccggg aactgaaagt cccctgcgtt atcggcgctc aaacagcgac
     1381 tgatgttttg aaagatgatg accgggttga agttgacgcc ggaaaaggta taataaagaa
     1441 actatgaaaa acaaaatatt aaaaataatt ttaatcatcg tgatcacgct ggcggtttta
     1501 atttatttaa ttttcagttt tgtcaataag agaaacacca tcaaagatgt tttaaatggc
     1561 cagtatcaga tagtttcaat gataactgac ggccagtcgg agattattga cggcgatgaa
     1621 ataattaatt ttaaaaaaga tgggagctgg gaaagatcag gcgttggcgg caactggcgc
     1681 ggtaattatt ttattcagga cggacagatt gttttcagtg attatgaaga tgaccagggt
     1741 caaaagcagg atcaatcttt gcaaggacaa ctccagcttg acggtaagga tttaattatc
     1801 acttatggcc agcaggcttt ttggacgcag agaatagttg tttatgaaaa actatagaat
     1861 aatgttattc ttaatataag aagaggttct tttataatta atcattaagt cgaaaatata
     1921 tgaagtattt gtcatatatg ctgtttggtt ttttagcttt tagcctgctc tttggggcta
     1981 atttagcggg agctcaaaca gccgctacga ccacttcagc gtcagccgag gcccaagtca
     2041 atcttgaggc tgaacttccc aaagtccggg ttaacccagt ttcagtttgg taccgggtta
     2101 agctcgcctg ggaaagagtt gtagacgttt ttgtcagcgg agaggcccaa atcagacgac
     2161 accttctcct tacaaaaaaa cggttggctg aagcccgggc atcagttaac accagggctg
     2221 aagcccagat cagcgccgtg gttgaaagaa gccggcggcg ttctgaagcc gctttggaac
     2281 tttatgaatc aattaaagtc agactggaca gtgaggcccg agcccgggtt ttggctgaac
     2341 ttaacgcgac tgcccgagtc caggccgaag taatagctga gatttacgga gaagttagcg
     2401 cggaaacccg cgccggatta gacgctctga ttgatgtttc caacagatca ctccaaaggc
     2461 ttgagaattc aaccgaagag cttaaaatgg aacttcgcag cgatgattcg gctgaagctg
     2521 aaattaaagc ccgattggag gcatttccgg taattgagtt tcgggaaggc tcaacttcag
     2581 aaatagattc aacgcaggaa tcagaccgga cggaagaaga gtca
//
LOCUS       DASWHZ010000075         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3475690, whole genome shotgun sequence.
ACCESSION   DASWHZ010000075 DASWHZ010000000
VERSION     DASWHZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475690"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VGA49_01710"
     CDS             complement(<1..1087)
                     /locus_tag="VGA49_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664518.1"
                     /translation="MKYSTKILIVALLLILPLTSLEAAVFNPNHIISDSEMVDFDSFN
                     LEAIQKFLESKTGTLKDYIAPDKDDLMKKTSLIIYQVSQKYQINPKYLLTLLQKEQSL
                     IDDPVPVQDQYDWATGYAICDVCSKSDPTLQRFKGFAQQIHSAAAQMRYYLDHPEEFR
                     FVTGQTYDIDGHLISPVNIATASLYNYTPHYHGNLNFWKIWQGWFAKSYPDGSLLQAE
                     GEPGVWYIQHDLRRPILSWSALISRFDPKKIIIVDPSDLQRYDIGLPIKFPEFSLLKS
                     PDNTIYLLVNDQLRPVSSMDVFKTIGFNLDEVTEVELAEIGRYRLGQPITIKSVYPMG
                     ALIQNNQTGGIYYVKDGFKFPVWSKEIM"
     gene            complement(1106..>1844)
                     /locus_tag="VGA49_01715"
     CDS             complement(1106..>1844)
                     /locus_tag="VGA49_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9664519.1"
                     /translation="KEDSLIRIDMSEYMEKHSVSKMIGSPPGYVGYEEGGQLTELVRH
                     RPYSVILFDEIEKANPEVFNLLLQILDEGRLTDAKGRAVNFKNTIIIMTSNLGNQVID
                     SYAIGFDAKAEEPGSNESDYEQMKEKIMEVMQDNFKPEFLNRIDDIIIFRHLLAADLI
                     KIVDLQLEKVVARLDDKNINLKVSRSAKEFLAKKGYDKLYGARPLKRVIQNKILDGLA
                     LKIVEGKISDGDEVNIDCQKDEIYIKS"
BASE COUNT          543 a          386 c          352 g          563 t
ORIGIN      
        1 gcataatttc tttggaccag acaggaaatt taaaaccatc tttgacgtaa taaattccgc
       61 cagtctgatt attttgaatc agcgccccca ttggataaac gctttttatg gtgattggct
      121 gacccaaacg atagcggcca atttcagcca attcaacttc agtcacttcg tccagattaa
      181 aaccaatggt tttaaaaacg tccattgaac tgactggccg gagctggtca ttgaccagca
      241 gataaatagt gttgtctggt gattttaaca acgagaattc cggaaactta ataggcaggc
      301 caatatcata ccgctgtaaa tccgaagggt caactataat aattttcttt gggtcaaacc
      361 gggaaattaa agctgaccag gataaaatcg gtcgtcttaa atcatgctga atataccaga
      421 cgcctggttc gccctccgcc tgaagcagcg aaccgtcagg atatgatttg gcaaaccagc
      481 cctgccagat tttccaaaaa ttaagatttc cgtgataatg aggagtatag ttgtataaag
      541 aagccgtggc gatattaacc ggtgagatca gatggccgtc aatgtcataa gtttgtcccg
      601 tcacaaaccg aaattcttcc ggatgatcca gataatacct catttgagcg gcggcgctgt
      661 gaatctgctg ggcaaagcct ttaaatcgtt gtaatgttgg atcgcttttg gaacaaacat
      721 cacaaatcgc ataacctgtc gcccagtcat actggtcctg gactggaacg ggatcatcaa
      781 tcaggctttg ttctttttga agcagagtta aaagatattt agggttaatt tgatattttt
      841 ggctgacctg ataaataatc agagaagttt ttttcatcag atcatctttg tcaggagcga
      901 tataatcttt cagagtgccg gtttttgatt ccaaaaattt ctgaatcgcc tccagattaa
      961 aggaatcaaa atcaaccatt tcactatcag aaataatgtg atttgggtta aaaacagccg
     1021 cttccagact ggttagaggc aatattaaca ataaagccac aattaatatt ttagttgagt
     1081 atttcatggc tttattatag cttatttacg atttaatata aatttcatct ttttgacaat
     1141 caatattgac ttcatcgccg tcagaaattt taccttcaac tattttcaaa gccaggccat
     1201 ccaaaatttt attctggatg actcttttta gaggccgggc gccgtacaat ttatcatagc
     1261 cctttttagc cagaaattcc ttggctgacc gggaaacttt taaattaata tttttgtcat
     1321 caagtcgggc gacaactttt tccagctgca aatcaacaat ttttattaaa tcagccgcca
     1381 gcagatggcg gaaaataatt atatcgtcaa ttcggtttaa aaactccggt ttaaaattat
     1441 cctgcatcac ttccatgatt ttttctttca tctgctcata gtcagattca ttacttccag
     1501 gctcttctgc cttggcgtca aaaccaatcg cgtagctgtc aatgacctgg ttgccaagat
     1561 tggaagtcat aattataata gtatttttaa aattgactgc ccggcctttg gcgtcagtca
     1621 gccggccctc atccaggatt tggagcaaaa gattaaaaac ttccggattg gctttctcta
     1681 tttcatcaaa aagaattact gaataaggcc gatgccgaac cagttcagtc agctggccac
     1741 cttcctcata accaacatag ccgggagggg agccgatcat tttagaaact gaatgttttt
     1801 ccatgtattc tgacatgtca attctgatca gcgagtcttc cttg
//
LOCUS       DASWHZ010000076         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_543476, whole genome shotgun sequence.
ACCESSION   DASWHZ010000076 DASWHZ010000000
VERSION     DASWHZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543476"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGA49_01720"
     CDS             complement(<1..271)
                     /locus_tag="VGA49_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX9664520.1"
                     /translation="MVRTDGGSDMTGIVAVYKPKGPTSNDVVQIIKKATGEKVGHAGT
                     LDPLAEGVLVVGIGQEYTKKLKDIVQSEKEYIAKIKLGQTSATDDE"
     gene            complement(241..1512)
                     /locus_tag="VGA49_01725"
     CDS             complement(241..1512)
                     /locus_tag="VGA49_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEX9664521.1"
                     /translation="MKKVYLLVDCNNFFVSCERVFKPALKNKPVMVLSNNDGCVISRS
                     NEVKKFGVPMGAPVFKYRDLIKKYNIRVFSTNFTLYGDMSNRVMETLEYFTDDLEIYS
                     VDEAFFKLNQWTGGHLENYGRLIKTTVQKWTGIPVSIGIGSTKTLAKAANELAKKHEE
                     FSGVLSLVNNSRTDQLLAQLAIGDVWGIGRAYRQSLPPYGLKTALDLKNAPDFFIKEK
                     MGVMGERTVLELRGVSCVELDHGPTVRKTILTSRSFRDPLTSLSQLKKAIAKYVSRAA
                     EKLREQGSVAGSLNVFITTSRFKKDNYYSNSANTKFLSPTSYTPTMIASALKNLEKIF
                     KPGCLYKRAGVLLTDISSTDNIQLNFFNPYSENKKQQRLMASLDEINDRWGGDTVKSA
                     AMGVNRIGRYNVLKKSASYTTKWLELMVVQI"
     gene            complement(1515..1943)
                     /gene="umuD"
                     /locus_tag="VGA49_01730"
     CDS             complement(1515..1943)
                     /gene="umuD"
                     /locus_tag="VGA49_01730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HEX9664522.1"
                     /translation="MKITQIFAFQHKVKHQLPLYVEGVSAGFPSAADDYIETKLDLNE
                     HLIKHPAATFFVRVEGSSMIKAGIQSGDILIVDRALEAGDNQIVIALINGEFTVKRIS
                     HDKNKVYLTPANPAFQPIEVTSEMDFEVWGVVTYVIHQAK"
     gene            complement(2159..2509)
                     /locus_tag="VGA49_01735"
     CDS             complement(2159..2509)
                     /locus_tag="VGA49_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664523.1"
                     /translation="MFSYSRDICSRNHRRFTVCGSHHIEGHKGDWKICRKCREDFEPE
                     MYVWYGMNEYNFERLPNPPAFEPTYCSKCLERIVLPDGGYSLLCGVYRCDNCPITDNE
                     REEIIQEYEKSKGK"
     gene            complement(2738..4213)
                     /locus_tag="VGA49_01740"
     CDS             complement(2738..4213)
                     /locus_tag="VGA49_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9664524.1"
                     /translation="MAKNTKKYLEALGIHNLRQALNAYNPWWRTKSFSVPGVPDFKRD
                     AFIQAMRHTEANHGLALLINGPRRVGKTTIINQVIRCLIEEKRIPTNRILFFSLDDPI
                     IQQLPQKDQGTLFEALLAQWAQVAGSALRTSPNTLYCFLDEVQRLPRWELYIKRYVDL
                     HYPIRFIISGSASHTIFRKSLESLLGRLVDISLPPFTFREFVRFYYPENRAFLTKLSD
                     DVLDIGDVASVIKFNRRLSEEIKPSHIQQWNHYADEYAQKGGFPQLWTMVTAAERSAF
                     IDQQFVERVTLEDLRLVKEIRRPEIFHQFLRYAFARTGQEYNLEELASLIHTTRVTLT
                     EALPLLLQTELIRKVERFTGKPVRLRSTHAKLYAADLVLMQGITKIITSLEGEERGRV
                     AETLAHNVIRLFPGVSDVSYYREPSGKREVDFIVRVGSRMMPIEIAYQNQLDNKHRIA
                     IRTFLEAHGKPNSFGILVTKEDWKIDQPILEMPLSLFLLTA"
     gene            complement(4279..4662)
                     /locus_tag="VGA49_01745"
     CDS             complement(4279..4662)
                     /locus_tag="VGA49_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX9664525.1"
                     /translation="MFYNVTLPVTLWFLDRGKKHVPYGTEGSRSGKTDRKNKILFIDA
                     RNIYRQIDRAHRDWTDEHIEQIASIVRSYRSKNGKKYKDVKGLCKVATIDEVKKAGYS
                     LNPGCYVGVTDNGDKDDENLKKIEL"
BASE COUNT         1468 a         1152 c         1032 g         1617 t
ORIGIN      
        1 cttcatcatc agtggcgctg gtctggccca gtttgatttt ggcaatgtac tctttttcag
       61 actgaacaat atcttttaac tttttggtgt attcctggcc aatccccacc accaaaactc
      121 cttcagccaa aggatcaaga gtgccggcat ggccgacttt ttcgccagtc gcttttttaa
      181 ttatctgaac gacatcattg gaagtcggcc cttttggttt gtaaacagcg actatgcctg
      241 tcatatctga accaccatca gttctaacca tttagtcgta tagctggctg atttttttaa
      301 tacattataa cggccgatcc gattaacgcc catggcggct gatttgaccg tgtcaccgcc
      361 ccagcgatca ttgatctcat caagactggc catcagtctc tgctgttttt tattttcgct
      421 gtaaggatta aaaaaattta gctgaatatt atctgtggag gaaatatcag ttaataaaac
      481 cccggctctt ttatataaac aacccggttt aaatattttt tctaaatttt ttaaagcaga
      541 ggcaatcatt gttggagtat agctggttgg cgataaaaat ttagtattag ccgaattgga
      601 ataataatta tcttttttaa accggctggt ggtgataaaa acatttaaac tgccggcgac
      661 tgatccctgt tctcgtaatt tttcagccgc ccggctgaca tatttagcga ttgctttttt
      721 aagctggctt aaactggtta aaggatctct gaaagatcgg gaagttaaaa tagtttttct
      781 gactgttggg ccgtgatcca attcaacgca cgagacgcct cttaattcca aaactgtccg
      841 ctcccccatc acccccattt tttctttgat aaaaaaatcc ggagcgtttt tcagatccaa
      901 agctgttttc aggccgtagg gcggcaggct ttgcctataa gcccggccaa tcccccagac
      961 atcgccaata gccaattgag ctaataactg atcggttcgc gaattattaa ccaaacttaa
     1021 aacgccgcta aactcctcat gctttttagc cagttcattg gcggctttgg ccagggtttt
     1081 agttgatccg atgccgatgg agacaggaat gccggtccat ttttgaacag ttgtcttaat
     1141 caaccggccg tagttttcca gatgaccgcc agtccattgg tttaatttaa aaaaagcttc
     1201 atccactgaa tagatttcca aatcgtcggt aaaatattcc agagtttcca ttacccggtt
     1261 agacatatcg ccatagaggg taaaattggt tgaaaagacc cggatattat attttttaat
     1321 taaatctctg tatttaaaaa ctggcgcgcc catcggcaca ccaaattttt tgacttcatt
     1381 ggaccgggaa ataacacagc cgtcattatt ggacaaaacc atcaccggtt tattttttag
     1441 agccggctta aaaacccgtt cgcaggaaac aaaaaaatta ttgcagtcaa ccagtaaata
     1501 aacttttttc ataattactt agcctggtgg ataacataag tcaccacgcc ccagacttca
     1561 aaatccattt ctgatgttac ttcaatcggt tgaaaagccg ggttggccgg cgttaaataa
     1621 actttatttt tatcatgact gattcgctta acagtaaact cgccgttaat cagagcgatc
     1681 acaatctgat tatcccccgc ttccagggcc cggtcaacaa ttaaaatatc gccggactga
     1741 ataccggcct tgatcatgga actgccttcc acccggacaa aaaaagtggc cgccggatgt
     1801 ttgatcaaat gttcgtttaa atccagcttg gtttcaatat agtcgtcagc cgctgacggg
     1861 aagccggctg acacgccctc aacatacagc ggcagttgat gtttaacttt gtgttgaaaa
     1921 gcgaagattt gagtgatttt catagagcct tttcatttca tgggtaaaat ttaaactacc
     1981 gcaaatggtg attttcataa atttattatt gtttatgtca atatatagtg aattatatca
     2041 aaaaagagat aaaatgcact acttttatct catatccggg gttagttttg attatttttc
     2101 aaatactctt ttattctctg acgattttca ggtgattttg tttgtagcat attaaaaatt
     2161 acttgccttt ggatttttca tactcctgaa taatttcttc tcgttcgtta tcggtgattg
     2221 ggcagttatc acaacggtat actccgcaga gtaatgagta tccgccatct ggcagaacaa
     2281 ttctttctag gcatttggag cagtaagtcg gttcaaaagc aggtggattt ggaagtctct
     2341 caaagttata ttcattcatc ccataccaca cgtacatttc cggctcaaaa tcctcgcggc
     2401 attttctgca aattttccaa tcgcccttat gtccctctat gtgatggctt ccacagactg
     2461 taaatcgccg gtgattgcgc gaacaaatat cacgcgagta cgaaaacatg acatactcgc
     2521 tttcatcgcc acaaacccaa ttgccgcagc atttcgtttt gtatctcggc ttttttgttt
     2581 tcccgcacaa cccgcatggc ggatttatgg tttttaccct agttttcttt attgtttttg
     2641 acatatgaaa ataaattctc taaaaatagc ccaatttaag agggcaaaat acctttatta
     2701 gatttattat caacccccct tgaaaataaa taatttatca tgccgtaagc aaaaataatg
     2761 ataacggcat ttctaaaatt ggctgatcaa ttttccaatc ctcttttgta acaagaatgc
     2821 caaagctgtt tggctttccg tgcgcctcca aaaatgtcct gattgcgata cggtgtttgt
     2881 tgtctaattg gttttgatac gcaatttcaa tgggcatcat ccggctaccg actcgcacta
     2941 taaaatccac ttcccgtttt ccgctgggtt cccgataata cgatacatcg gaaacgccgg
     3001 ggaataatct gatgacatta tgcgccagcg tctccgcaac tctgccgcgt tcttctcctt
     3061 caagagaggt gataattttg gttattccct gcatcaggac aagatcagcc gcatataact
     3121 tcgcatgggt agagcgcaaa cgcaccggct tgcctgtaaa acgttccact ttgcggatta
     3181 attccgtttg caaaagaagg ggcaacgcct ccgtaagggt tacgcgcgtt gtatggataa
     3241 gtgaggccag ctcttcaaga ttatattctt gccccgttcg cgcaaatgca tagcgtaaaa
     3301 actgatggaa aatttccggc cgccgtatct ctttcaccag tcgcaaatct tccagtgtaa
     3361 cgcgctccac aaattgttga tcaataaacg cggagcgttc ggcggcggtt accattgtcc
     3421 ataattgagg aaagccgccc ttctgcgcat actcatctgc gtaatgattc cactgttgaa
     3481 tgtggcttgg tttaatttct tcacttaaac ggcgattaaa cttaatgact gatgcaacat
     3541 ccccaatatc caaaacatcg tctgaaagtt ttgtcaaaaa agcacgattc tcgggataat
     3601 agaaacgaac aaattcacga aaggtaaacg gtggtaaaga aatatcaact agccgaccaa
     3661 gcaagctctc aagtgatttt ctaaaaatgg tatgagaagc agagccggaa ataataaagc
     3721 ggataggata atgcaagtca acatatcgtt taatgtacaa ttcccaacgt ggtaagcgct
     3781 gaacctcatc taaaaagcaa taaagtgtat ttggcgatgt acgcaacgcg gaaccagcaa
     3841 cttgagccca ttgagcaagc aatgcctcaa atagtgtgcc ctgatccttt tggggtagtt
     3901 gttgaataat tggatcgtca agcgaaaaaa acagaatgcg atttgttgga atcctttttt
     3961 cctcaatcag acaacgaatg acttgattga ttatcgttgt ctttccaacg cggcgagggc
     4021 cattgattaa aagggcaagg ccgtgatttg cctctgtatg acgcatagct tgaataaacg
     4081 catccctctt aaagtcagga acgcccggca cagaaaaaga ctttgtccgc caccagggat
     4141 tataggcgtt taacgcttgc cgcaagttat gtataccaag cgcttcaagg tattttttcg
     4201 tatttttagc cataatttat ggttttatat ctatgtatac tatactatac ataactaata
     4261 acaatgtcaa tatcctccct ataactctat tttcttcaaa ttctcatcat ccttgtcacc
     4321 attatcagta acaccaacgt aacagccagg attgagtgag tagccggctt tctttacttc
     4381 gtctattgtt gctactttgc aaaggccctt tacatcttta tacttttttc cattcttact
     4441 gcggtagctt ctcacgatgc ttgcgatttg ctctatatgt tcatcagtcc agtcacgatg
     4501 cgcacggtct atttgccggt aaatattgcg agcgtcaata aaaagaattt tgtttttgcg
     4561 atcggtcttt cccgaacgag aaccttcggt tccgtacggg acatgctttt tgcctctatc
     4621 caaaaaccaa agcgttaccg gaagcgtcac attgtaaaac atgttcgagc ccgacagtga
     4681 caaaccccgc gatactatta atatttattg gcacagcctc aataacagaa taatttaata
     4741 atgtttcttc gcaggctgat aaaatctgat caacattttt cacggggaaa tgcgggagaa
     4801 tacagagtga tgtgcgaata gaaagaaata tcatccagta taaaataaac agagtgacgg
     4861 ctggcaatat cccggcttat ttctatcgcc cgattgcgaa tatcctgggg aggatataag
     4921 gctatattaa ggctggtgaa ttcaccattg ttcatattga aaaatttatt cttgggagca
     4981 gggattttcg tttgtggggg ccgggtgggg ctacaaaact tgttttgagt gaagccggag
     5041 gatcaaaggg tggtgggtgt ggttagattg taattcggta ttcaaagaag ttcagcgagt
     5101 tcctcttgct cgtcctcgtc aaattcgtcg agatcaaacc cctcgctttc caatttttct
     5161 tttatttctt tttgccgctc ttgttcttcg tccataaaat tattatagag gtaaattttc
     5221 acccctttct cggctcattg atttggaacg ccattagtgt tagataaat
//
LOCUS       DASWHZ010000077         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3139083, whole genome shotgun sequence.
ACCESSION   DASWHZ010000077 DASWHZ010000000
VERSION     DASWHZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139083"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..645
                     /locus_tag="VGA49_01750"
     CDS             124..645
                     /locus_tag="VGA49_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664526.1"
                     /translation="MQQIGQFLSSINWAAPTWDLFIILFFIIAAFIYGLSLGRDRVIV
                     IMVGIYMALAVVNNAPFINAGAGAQIGIYDFYVFKVTAFIATFIILFFLLSRSSLLRA
                     ISNIEISAGLFQVLIFSLLHVGLFVSITLSFLPPEAVNNLSGLTRQVFVNETSQFLWI
                     IAPIVFMGILKSD"
     gene            complement(740..2122)
                     /gene="cysS"
                     /locus_tag="VGA49_01755"
     CDS             complement(740..2122)
                     /gene="cysS"
                     /locus_tag="VGA49_01755"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9664527.1"
                     /translation="MNTLYLFNTASRRKEKFKPIKKNQVGLYSCGPTVYNYAHIGNLR
                     SYVFADILKRTLKYNGYKVKHVINITDVGHLVSDESAGEDKMIVAARCEKKTVWQIAQ
                     FYTDAFKKDIKKLNILPPDIWAKATDHIKEQIDLIKKLEKTGYTYVIEDGVYFDTAKF
                     KDYPKFARLDVKGLKAGARIEMVKGKKNLTDFALWKFSPKDKQREMEWTSPWGKGFPG
                     WHIECSAMSTKYLGEHFDIHTGGIDHIPVHHTNEIAQSVCATGQKFVNYWLHNDWLVI
                     GQGVKMAKSGQNFIRLEDLINEDYRPLAYRYLLLGASYRSKLEFSKESLTAAQTALDK
                     LHDSFLELPASPDKSGLKYEKKFLQAINDDLNTPQALAVVWNYIKSSKADKKTLLKFD
                     KVLGLGLDKIKKKKVAIPANIKKLVQDREQARQAKNWKKADQLRNKIEKAGYLVEDTP
                     RGPIIKTLKH"
     gene            complement(2214..3149)
                     /locus_tag="VGA49_01760"
     CDS             complement(2214..3149)
                     /locus_tag="VGA49_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX9664528.1"
                     /translation="MRVRDNIEKFKHKDFFWLNITKKTAKEIDYLRKHFDFHPSDLRD
                     TLPPLQRPKLIEHHDYIFLILLFPLYSKRKKTIVSSEIDFYIGPDYLITIHENDLSPI
                     RKFTEQVRKNPGLRQTYLEQGGGRLLYEILYPLFTSTFPLLTKIGNRLDMVEKQIFAV
                     HRRETIHQILEIKTNIVDLRKTMQSHKNVLKKIINKSPDVLPAVRFEKNKFNSLKEIS
                     IDIWDYLETFNNTIDAVHQTHESLANFRLNDIIKTLTIFSVIVFPLTLLAAIFGMNTP
                     GIPFTDSAYGFWIVVAIMAVGTLGMFYFFRHKRWI"
     gene            3309..>3871
                     /locus_tag="VGA49_01765"
     CDS             3309..>3871
                     /locus_tag="VGA49_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664529.1"
                     /translation="MIAVSIQQIFRLALFGLVFFIQSVSAGEKSEESSFYDYMAEQQA
                     ALTDEDYSWFKFELDDWLDFAGENSSPFVEFMDGRPRAFDQQRLALLQLNVNPYDSFE
                     ANAKLWLTASQLFARGSDFLGEELGADDYVLARIGQALLSWYFNMTISGISHELGHYY
                     AIRTTNPDVEIIFAGFSVFWGPAMEYPT"
BASE COUNT          986 a          727 c          878 g         1280 t
ORIGIN      
        1 agcggaaaaa ggtttttggg agaaaatatt ttaaggggga taagtcttaa ataaattaag
       61 ccgcggcttg gttttttgtt gtaaaaaatt atgttataat aatttaatat aattcatttt
      121 actatgcagc aaatcgggca atttttaagt tcaattaatt gggccgcgcc aacctgggat
      181 ctttttatta ttttattttt tataattgcc gcttttattt acggtttgtc tttgggtaga
      241 gacagagtaa ttgtcattat ggtcggcatc tacatggcgc tggccgtggt taacaacgcc
      301 ccgtttatta acgccggcgc cggcgcccag attggaattt atgatttcta tgttttcaaa
      361 gtaaccgcct ttatcgccac ttttataatt ttattttttc tgttatcccg cagcagtttg
      421 cttcgggcga tttccaatat tgaaatttca gccggcttgt ttcaggtgtt aatttttagt
      481 ttgctgcatg tcggcttgtt tgtcagcatt actttgtctt tcctgccgcc ggaagccgtt
      541 aataacttat cagggctgac tcgtcaggtt tttgttaatg aaacttctca atttctctgg
      601 attatcgccc caatcgtttt tatggggatt ttgaagtcgg actaaagcaa caaatctaaa
      661 aaacaaaaaa tcaagaaagc ataaaagcat gaaagctata ttttgtttta atgttttaat
      721 gttttaatgt ttttttgttt taatgtttta atgttttaat gattggtccc cgcggtgtat
      781 cttccaccag ataaccggcc ttttcaattt tatttcttag ctgatcagct tttttccagt
      841 ttttggcctg ccgggcttgt tcacgatcct gaacaagttt ttttatatta gccggaatgg
      901 ctactttttt ctttttaatt ttatccagcc ccagtcccag aactttatca aattttaata
      961 aagttttttt atcagcctta cttgatttaa tatagttcca gacaacagcc agcgcctggg
     1021 gcgtgttcag gtcgtcattt atggcctgga ggaatttttt ttcatatttt aaacctgatt
     1081 tgtctggtga agccggtaat tccaaaaagc tgtcatgtaa tttgtccaaa gcagtttggg
     1141 cggccgttaa agattctttg gaaaattcaa gtttggagcg gtagctggcg cccagcaaaa
     1201 gataacggta agccagcggc cgatagtcct cgtttattaa atcttcaagt ctgataaagt
     1261 tttggccgga cttggccatt ttaacgccct ggccaatcac cagccagtcg ttatggagcc
     1321 agtaattgac gaatttttga ccggtggcgc agactgactg ggcgatttca ttggtgtggt
     1381 ggaccggaat atggtcaatg ccgccggtgt gaatatcaaa atgttcgccc agatatttcg
     1441 ttgacatggc cgagcattca atgtgccagc cgggaaagcc tttgccccag ggggaagtcc
     1501 attccatttc tctttgtttg tctttaggcg aaaatttcca taaagcaaaa tcagtcagat
     1561 tttttttgcc tttgaccatt tctattctgg cgccggcttt gagccctttg acatccaggc
     1621 gggcgaattt ggggtaatct ttaaatttgg ccgtgtcaaa atagacgccg tcttcaataa
     1681 cataggtata acccgttttt tccagttttt taattaaatc aatttgctct ttgatatgat
     1741 cagtcgcttt ggcccagatg tcaggcggta aaatattaag ttttttaatg tcttttttaa
     1801 aagcgtccgt ataaaattga gcgatctgcc agacagtttt tttttcgcac cgggcggcca
     1861 cgatcatttt gtcttcgccg gcgctttcat cagacaccag atggccgaca tcagtaatat
     1921 tgatgacatg ctttactttg taaccgttgt attttaaagt tctttttaaa atgtcagcga
     1981 aaacatagga acggaggtta ccgatatggg cgtaattata aacagtcggc ccgcaggagt
     2041 agaggccaac ttggtttttt ttgataggtt tgaatttttc ctttcgtctg gaggcggtat
     2101 tgaagaggta taatgtgttc atataaaagt taacagtcaa ctgccaacag tcatcagggg
     2161 cgggactggg attaaatccc tgttggctgc tggcggctga ctgttggctc aactcaaatc
     2221 catctcttat gcctgaaaaa gtagaacatt cccagcgtgc caacagccat aatggcgacg
     2281 actatccaga agccgtaagc agaatcggta aaagggatgc cgggcgtgtt cattccaaaa
     2341 attgccgcca ataaagtcag cggaaaaaca attaccgaga aaatcgtcag agttttgatg
     2401 atgtcattca gccggaaatt ggccagcgat tcgtgcgttt gatgaaccgc gtcaatggta
     2461 ttattaaatg tttccagata atcccagatg tcaatggaga tttctttgag gctgttaaat
     2521 ttattttttt caaaccggac tgctggcaga acatcgggcg acttattgat aattttttta
     2581 aggacatttt tatggctctg cattgttttg cgcagatcaa caatgttggt ttttatttcc
     2641 aggatctgat ggatcgtttc ccggcggtgg accgcgaaaa tttgtttttc aaccatatcc
     2701 agccggttgc ctattttggt cagcagggga aaagttgaag tgaacagcgg ataaagaatt
     2761 tcataaagca ggcggccgcc gccctgttcc aggtaggttt gtcgcagacc gggatttttt
     2821 ctgacttgtt cggtaaattt tctgatgggg gacaggtcat tttcatggat agtaattaaa
     2881 taatcaggac caatgtaaaa atctatttct gaagaaacaa ttgttttttt ccgtttgcta
     2941 taaagaggaa aaagcaaaat taaaaaaatg taatcgtggt gttcaattaa tttgggtctt
     3001 tgaagcggcg gcaaagtgtc ccgaagatcg gacggatgaa aatcaaaatg tttgcgcaaa
     3061 taatcaattt ctttggcggt ctttttggtg atattcagcc agaaaaaatc tttgtgttta
     3121 aatttttcaa tattatcccg aacccgcata atagttttgt tttgatgttt gaatccttat
     3181 atatattata gtatctttgg cttaggttga caagtggcta tttttatgat attttgtggg
     3241 gtcaaaaagt tctttgaaag attaggaatt gtgacaggtc aaaacaggcc tgcggaagga
     3301 gattcagaat gatcgcagtc agcattcagc aaatttttcg gttagcgttg tttggtttgg
     3361 ttttttttat ccagtccgtc tcggctggcg agaaaagtga agaatcgtca ttttacgatt
     3421 atatggcgga gcagcaggcg gctctgactg acgaggatta ctcctggttt aagtttgagc
     3481 tggacgactg gctggatttt gctggcgaaa acagttcccc gtttgtcgag ttcatggacg
     3541 ggcggccccg ggcttttgat cagcaacgac tagccttgct tcagctcaac gtcaatcctt
     3601 acgacagttt tgaggccaac gccaagctct ggctgacagc cagccagttg ttcgcccggg
     3661 gcagtgattt tttgggcgaa gagctgggag cggacgatta tgttctggcc aggattggcc
     3721 aggctttgtt gtcctggtac tttaacatga cgattagcgg aatttctcat gagcttggtc
     3781 actactacgc catcagaacc actaatccag atgttgaaat aatttttgct ggttttagtg
     3841 tgttctgggg accagcaatg gaatacccta c
//
LOCUS       DASWHZ010000078         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2033986, whole genome shotgun sequence.
ACCESSION   DASWHZ010000078 DASWHZ010000000
VERSION     DASWHZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033986"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VGA49_01770"
     CDS             complement(<1..754)
                     /locus_tag="VGA49_01770"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX9664530.1"
                     /translation="MPLSLAFILSVVLTVAVRKAALRYKILDYPGGDRKIHQKPIPLL
                     GGLAIFLSFFIIVLYYAFFTDNILDGIRLKNLIGVLIASAVIMIGGFLDDKYNLKPKY
                     QIIWPILAVIIVIAGGLGVTDITNPFGGVFELDQWKFVLFWYQGTPAQVFILADVFTM
                     VWLLGLMYTTKFLDGLDGLTTGVSAIAALIIFFLANTEKFFQPPMAYLAIIFFGAGLG
                     FLIFNFHPAKIFLGEGGSLWTGFMLGVLSIIAG"
     gene            complement(774..>1714)
                     /gene="tgt"
                     /locus_tag="VGA49_01775"
     CDS             complement(774..>1714)
                     /gene="tgt"
                     /locus_tag="VGA49_01775"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9664531.1"
                     /translation="MLRPGVKVIKKFGGLHQFMGWSGPILTDSGGYQVFSLAQIRKIK
                     EEGVEFKSEIDGAKVLLTPEKAIEVQLILNSDILMVLDECPPWPCAKKYAKKSLEMTT
                     RWARRGKEHFSNLIIKQSAKGGKKNLMFGIVQGSTYKDLRLESARQLLELDFDGYAIG
                     GLAVGEPRRQMYQVLDYLVPALPPNKPHYLMGVGKPEEIIRAVQKGIDMFDCVIPTRN
                     ARHGLLYVFNRRKKKLTGDFYQTIKITNQRYQADTKPPDSRCGCYCCRHYSRAYLRHL
                     FMTKEFLGLRLATIHNLFFYLNLMAGIRQEIRAGQL"
BASE COUNT          526 a          416 c          345 g          427 t
ORIGIN      
        1 tgccggcaat aatcgataag acgcccagca taaaaccagt ccacagactg ccgccttctc
       61 ccagaaatat tttggccgga tgaaaattaa aaattaaaaa tcccaggcca gccccgaaaa
      121 aaattattgc cagataagcc atcggcggct gaaaaaattt ttcggtgttg gccagaaaaa
      181 aaatgatcag agcggcgatg gcgctgacgc cggttgtcag tccgtccagt ccgtccaaaa
      241 atttcgtggt gtacattaaa cccaaaagcc agaccatggt aaaaacatct gccagaataa
      301 aaacctgggc cggcgttccc tgataccaga ataaaacaaa tttccactga tcaagttcaa
      361 aaactccacc gaaaggattg gttatgtccg tcacacccaa accgccggca atcactataa
      421 tcaccgccaa aattggccaa ataatctgat actttggttt taaattatat ttatcatcca
      481 aaaaaccgcc gatcatgata accgctgaag caatcagaac gccaattaaa ttttttaatc
      541 tgatgccgtc taaaatgttg tcagtaaaaa aagcgtaata taaaacaata atgaaaaaac
      601 ttaaaaaaat cgccaagccg cccagcaaag gaatgggctt ttgatgaatt tttctatccc
      661 cgccgggata atcaagaatc ttatatctta aggctgcttt tctgacagcc actgtcaaaa
      721 caactgataa aataaaagct aaactcagcg gcataaaata agcgaccata aaattataac
      781 tgaccggccc tgatttcctg cctgatgccg gccattaaat tcaggtaaaa aaataaatta
      841 tggattgtcg ccagccggag accgagaaat tccttagtca taaacaaatg acggaggtaa
      901 gcccgggaat agtggcgaca gcaataacag ccgcagcggc tgtccggagg cttagtgtca
      961 gcctggtagc gctgattggt tattttaatt gtctgataaa aatcgccggt taattttttc
     1021 ttccggcggt taaaaacata gagcaggccg tgacgggcgt tgcgggtcgg gataacgcag
     1081 tcaaacatgt caattccttt ttgaacagcc ctgataattt cttcgggctt gccaacgccc
     1141 attaaataat gcggtttatt cggcggcaaa gccggcacca gataatccaa aacctggtac
     1201 atctgccggc gcggttcacc caccgccaga ccgccaatgg cataaccgtc aaaatcaagt
     1261 tctaataact gccgggcgct ctccagtctt aagtctttat aagttgaccc ctggacaatc
     1321 ccaaacatca aattcttttt tccgccttta gcggattgtt taattattaa attgctaaaa
     1381 tgttctttac cgcgcctggc ccagcgggta gtcatctcca gagacttttt ggcgtatttt
     1441 ttggcgcaag gccagggcgg acactcatcc aaaaccatca gaatatcgct gtttaaaatc
     1501 aactggactt caatggcttt ttcaggcgtc aataaaactt tggcgccgtc aatttctgat
     1561 ttaaattcaa cgccttcttc ttttattttc ctgatctggg ccagactgaa aacctgatac
     1621 ccgccgctgt ctgtcagaat cgggccggac cagcccataa actgatggag gccgccgaat
     1681 tttttaatga ctttgacgcc cggccggagc atca
//
LOCUS       DASWHZ010000079         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3480864, whole genome shotgun sequence.
ACCESSION   DASWHZ010000079 DASWHZ010000000
VERSION     DASWHZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3480864"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VGA49_01780"
     CDS             complement(<1..264)
                     /locus_tag="VGA49_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664532.1"
                     /translation="MNKFWLILLIIFLIIFNLFILYLLIPEENYNPENKIDFGVTFSK
                     AHAQYFDLDWQQLFIDILDDLKIKKLRLSAYWDEIEPVEGQFDF"
     gene            complement(266..1159)
                     /locus_tag="VGA49_01785"
     CDS             complement(266..1159)
                     /locus_tag="VGA49_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9664533.1"
                     /translation="MSWILFAIIGYLFLALTGIIDKLLLSNFIRNTSVYVLYISLLGL
                     FALILIPFGFSLPATNILIASLIAGFLFLVALAFFFYALQVEEASRVIPLAGGLVPLF
                     VFIISYFFIGERLGVYQLIAFLCLVLGSVIITYEKKDPGQKIRRGYEITVLASFLFGL
                     SFVLTKYVFNAESFINGFIWMRLGGILIIPFLVWKAANRHDIFGNIRNVQGRILALFI
                     GAQAIGGLGFLSQNYAISLSSVTLVNALQGVQYVFVFILAALVTISFPKRFREKITGF
                     TLIQKIIALILIALGLYFITL"
     gene            complement(1390..2751)
                     /locus_tag="VGA49_01790"
     CDS             complement(1390..2751)
                     /locus_tag="VGA49_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664534.1"
                     /translation="MTKTKTNKQTKKNPKAWVVSVDMGYGHQRAAYPLKYLAYQKIID
                     ANNYRGIPERDKNIWRQSRRFYEFISRFKKVPVVGRWAFNVFDKLQHIEDFYPKRDLS
                     NSTFQVNRVVNLIKDGWGKHLIDKLEKNPLPLVTTFFVPAFMAQIHDYSREIYCLATD
                     TDISRSWVMDHPGVSRINYFAPSYRVVDRLILYGIRKDRIYLTGFPLPIENLGYDGLE
                     VLKNDLGYRLVNLDPKRKYLTEYRHAIEVEIGRQNIRRKPSHPLTLMFAVGGAGAQKE
                     LGIRIVQSLKTRIKRNEVNINLVAGIRNEVHQYFKSNVKALGLGAMLDKNIKIIYGNT
                     KPDYFEKFNKALRTTDIIWTKPSELSFYVALGIPIIMSPPIGSQEIFNKKWLTTIGAA
                     ISQENPKYADQWLFDWLDSGWFAEAAMHGVREATQYGAYNIEKIVSQKQEEVRELKTV
                     LQY"
     gene            complement(2898..2971)
                     /locus_tag="VGA49_01795"
     tRNA            complement(2898..2971)
                     /locus_tag="VGA49_01795"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2937..2939),aa:Trp,seq:cca)
     gene            complement(3026..>3868)
                     /locus_tag="VGA49_01800"
     CDS             complement(3026..>3868)
                     /locus_tag="VGA49_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9664535.1"
                     /translation="LLIILLVVAPISNFVVVLSKKSIDTYNLIQDRFTNGDLIEQVEA
                     KFRQLENYLSFVNPESFKDVVLNYSSQLNSFLISSTAGFIRGTTQFVTNLFLIILTLF
                     FFFRDGERLVKRLMQLTPLSNKYDRQIFKDFHNISYSTIVSSFVTAIAQGIVGGLGFY
                     LVGIPAFFLGVAMAFASLIPVGSAIIWLPTALILMAIGKAGSGLFIIIWGVLVVGLID
                     NVLRPVLIRGKVKIHPLFLFFSIIGGVAAWGFWGLIFGPIVLAIALTFIHIYEMEYEE
                     ILEK"
BASE COUNT         1284 a          853 c          728 g         1003 t
ORIGIN      
        1 aaaatcaaac tggccttcaa ccggctcaat ttcatcccaa taggcggaca gcctcaattt
       61 ttttattttt aaatcgtcca aaatatcaat aaacagttgt tgccagtcaa ggtcaaaata
      121 ttgagcgtgg gctttggaaa aagttactcc aaagtcaatt ttattttccg gattgtaatt
      181 ttcttcggga attaataaat ataaaataaa taaattaaaa attattaaaa aaattattaa
      241 caatatcaac cagaatttat tcatattaaa gggtaataaa atacaaaccc aaagctatta
      301 aaatcaaagc gataattttt tgaattaaag taaagccggt gattttttcc ctgaatcttt
      361 tgggaaatga aatagtcacc agggccgcca gaataaaaac aaaaacatac tgaacgccct
      421 gcagggcgtt aaccaaagta acactgctta gtgaaatagc gtagttttgg gataaaaacc
      481 ccagcccgcc aatcgcctgg gcgccgataa acagagccaa aatccgtccc tggacatttc
      541 tgatattgcc aaaaatatca tgccggttgg ccgccttcca gaccagaaaa ggaataatta
      601 aaataccgcc cagcctcatc cagataaatc cgttgataaa gctttcggcg ttaaaaacat
      661 atttggttaa aacaaaagat aaaccaaaca aaaaagaagc caggacggta atttcataac
      721 cgcgtctgat tttttgtccg ggatcttttt tttcataagt aataatgaca ctgccaagaa
      781 ccaaacacaa aaaagcgatc agctgataaa cgcccagtct ttcaccaata aaaaaatatg
      841 agataataaa aacaaacaaa ggaaccaggc cgccggccag aggaatcacc cgggaagcct
      901 cttccacctg aagagcgtaa aaaaagaaag ccaaagccac cagaaacaaa aaaccagcaa
      961 tcaggctggc gattaaaata ttggttgctg gcaggctaaa gccaaaagga attaaaatta
     1021 aagcaaacaa tcccagcagg ctgatatata acacataaac actggtgttc cggataaaat
     1081 tggacagcag gagcttgtca atgataccgg tcagagccag aaaaagataa ccgatgatgg
     1141 caaatagtat ccaggacata attcaaaaaa gtaagaagta aaaaataaaa agtaaaaaat
     1201 gaaaaattga agttctaagt tcaaataagt tcaaagataa agaaagttca aagttcaaag
     1261 tactaagcaa gcaaacctgg aactttgaac tttgaactta ctaactttga acttaaatct
     1321 aaataatcat cactgtttta atacttaaat cttaaatctt aaatcgagat tacttcttaa
     1381 tgccattcat taatattgca gcactgtctt caattcccta acctcttcct gcttctggct
     1441 gacgattttt tcaatattat aggcgccata ctgggtggct tcccggacgc cgtgcatggc
     1501 cgcttcggcg aaccagccgc tgtccagcca gtcaaacagc cactggtcgg cgtatttggg
     1561 attttcctgg gaaatggccg ctccaatcgt cgtcagccat tttttattaa aaatttcctg
     1621 agaaccaatc ggcggcgaca taataatcgg gatgcctaaa gccacgtaaa aactcaactc
     1681 ggacggtttg gtccagataa tgtcagtcgt tcgcaaggcc ttgttgaatt tttcaaaata
     1741 atcaggtttg gtgttaccgt aaataatttt gatattttta tcaagcatcg ctcccaggcc
     1801 cagggcttta acattggatt taaaatattg atgaacttca ttgcgaatgc cggccaccaa
     1861 attaatatta acctcatttc ttttgatccg ggtttttaaa ctttggacta ttcgaatgcc
     1921 cagttctttt tgagcgccgg ccccgccaac ggcaaacatc aaagtcagcg gatgactggg
     1981 cttgcgcctg atattttgcc tgccgatctc aacttcaatg gcatgccggt attccgttaa
     2041 atactttctt ttgggatcaa gattgaccag ccggtagcca agatcattct ttaaaacttc
     2101 cagcccgtca taacctaaat tttcaatcgg caatggaaac ccggttaaat aaatccggtc
     2161 ttttcttatt ccgtataaaa tcaaccggtc aaccacccga tagctgggcg cgaaataatt
     2221 gatccggctg acccccgggt gatccatcac ccagctccgg ctgatgtcgg tatcggtcgc
     2281 cagacaataa atttcccggc tgtaatcgtg aatttgggcc ataaaagccg gcacgaaaaa
     2341 agtcgttacc aggggcaaag gatttttttc aagcttgtca atcagatgtt tgccccagcc
     2401 gtctttgatt aaattaacca cccggttgac ctgaaaagtg ctgttggata agtcgcgctt
     2461 gggataaaaa tcttcaatat gctgaagttt atcaaaaaca ttaaaagccc agcgtccaac
     2521 gaccgggact tttttaaacc gggaaataaa ttcgtaaaac cggcggctct gacgccagat
     2581 atttttatca cgctccggaa taccccggta gttattagcg tcaataattt tttgataagc
     2641 caggtatttc agcggataag ccgcccgctg gtggccgtag cccatgtcaa ctgacactac
     2701 ccaggctttg ggattttttt tggtctgttt atttgttttt gttttggtca tagtaaataa
     2761 ttataccata aaatatcttt caaaataaaa aatcgtcctg ttaaggacga tttaatttat
     2821 gactttcgtt taaataatca accctgctcc accccgccct tcgcgaaggg cggggtggag
     2881 acccttgttt tataaattgg caggggcggt aggagtcgaa cccacgttac tggttttgga
     2941 gaccagagtt ctaccactga actacgcccc ctcattgaaa taaaaaattt cctatcacta
     3001 ttgcctcttg ccttgtgcct tgtgcctatt tctccaatat ctcctcgtac tccatttcgt
     3061 aaatgtgaat aaaagtcaga gcgatggcca ggacaattgg cccaaaaatc agaccccaaa
     3121 aaccccaggc ggccacgccg ccaataatgc tgaagaaaag aaacaacgga tgaattttaa
     3181 ctttgccgcg aattaaaacc ggtcgtagta cattatcaat taagccgacc actaataccc
     3241 cccaaattat aataaacaaa cccgacccag ctttgccgat agccatcaaa atcaaagccg
     3301 tcggcagcca gataatggcc gagccaacag gaattaacga ggcaaaggcc atggccaccc
     3361 ccaaaaagaa agccggaata ccgaccagat aaaaaccaag gccgccaaca attccctggg
     3421 caatagcggt cacaaaactg ctgacaattg ttgaatagct tatattatga aaatctttga
     3481 aaatctggcg gtcgtattta ttggacaacg gagtcagctg catcaaacgt ttaaccaaac
     3541 gctcgccgtc cctgaaaaag aaaaataaag ttaaaatgat cagaaacaaa ttagtcacaa
     3601 actgggttgt tcccctaata aacccggcgg tgctggaaat cagaaaactg ttcagctgag
     3661 agctgtagtt taaaacaaca tccttaaaac tttcagggtt aacaaatgac agatagtttt
     3721 ccaattgacg gaatttggct tcaacctgtt cgattaaatc gccgttggta aaccggtcct
     3781 gaattaaatt gtaagtgtca attgattttt tggataaaac caccacaaaa ttggaaatcg
     3841 gcgcgactac cagcaaaata atcagtaa
//
LOCUS       DASWHZ010000080         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3141949, whole genome shotgun sequence.
ACCESSION   DASWHZ010000080 DASWHZ010000000
VERSION     DASWHZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141949"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VGA49_01805"
     CDS             <1..212
                     /locus_tag="VGA49_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX9664536.1"
                     /translation="QWTAIQDAQKNGYKFYDFWGVAPDVVKIKREENWRGITRFKRGF
                     APTAPIVEYAGLFELAYRPRLYKWI"
     gene            203..1048
                     /gene="rsmA"
                     /locus_tag="VGA49_01810"
     CDS             203..1048
                     /gene="rsmA"
                     /locus_tag="VGA49_01810"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9664537.1"
                     /translation="MDLASPTTVKELCEKYDINPRRRQGQNFLIDQNVLNKIIQAADL
                     KKSDLVLEIGAGLGILTKELGERAKSVLTVEIDSRLIKALSETIGDYKNIIIIKEDVL
                     SKEFYQIFKKWLAKHSAGSYKIVANLPYNITSAVLRYFLEADLKPTEMILMVQKEVAE
                     RITAKPGQMSLLAVSVQFYGQPEIIDYVSKNSFWPSPKVDSAIIKLKTDQRYQVNPKI
                     FFRIVKIGFSAKRKQLQNNLSAGLKMDSQEIKEILKKIGLDEKIRAQDLAMEDWVNLI
                     GEIRN"
     gene            complement(1272..1619)
                     /locus_tag="VGA49_01815"
     CDS             complement(1272..1619)
                     /locus_tag="VGA49_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664538.1"
                     /translation="MLKNISWRKLIQNFRHLGFEGPYSGGKHLFIKKGHLKVHIPNHQ
                     PHRPAKAGRYAAVDPAVGGIYFLRPFIPAASRRVFWAKNKHSGDISPGLLNEILKQAG
                     IDKEDWNQMKAGE"
BASE COUNT          522 a          302 c          377 g          480 t
ORIGIN      
        1 tccaatggac agccattcag gacgctcaaa agaatggcta taaattttac gacttctggg
       61 gagtggcgcc ggacgtggtt aagatcaaga gagaagaaaa ctggcggggc attacccggt
      121 tcaagcgagg cttcgcgcca acggccccga tcgtggaata cgcgggtctt tttgagttag
      181 cttatagacc gagattatat aaatggattt agcctctcca acaacagtta aagagttgtg
      241 tgagaaatat gatattaacc cccgccgccg ccagggtcag aattttttaa ttgatcaaaa
      301 tgttttaaat aaaattatac aggcggcaga tctgaaaaaa tcagatttgg ttttggaaat
      361 cggggccggg ctggggattt tgaccaaaga gctgggtgaa cgggctaaat cagttttaac
      421 cgttgaaatt gattctcgtt taattaaggc gttgtcggaa acaataggtg attataaaaa
      481 cattataatt attaaagaag atgttttgtc aaaagagttt tatcaaattt ttaaaaaatg
      541 gctggccaaa cattcagccg gcagttataa aattgttgcc aacctgccct acaacattac
      601 atcagccgtt ctccggtact ttttggaggc cgatctgaag ccaacagaga tgattttaat
      661 ggttcaaaaa gaagtggccg agcgaattac ggccaagccg ggacaaatga gtttgctggc
      721 ggtttccgtc cagttttacg gccagccgga aatcattgac tatgtttcca aaaacagttt
      781 ttggcccagt ccaaaggttg attcagctat tatcaaatta aaaacagatc aacgatatca
      841 ggttaatcca aaaatatttt tcagaattgt taaaattggt ttctccgcta aaagaaaaca
      901 gctccaaaat aatttatcag ctggtttaaa gatggacagt caggagatta aagaaatttt
      961 aaagaaaatc ggcctggatg aaaaaatcag agcccaggat ttggcgatgg aagattgggt
     1021 gaatttgatt ggagaaataa ggaattaaag ggaagcaaag ggaagcagag ggaagcaaag
     1081 ggaattaaag ggaagcagag gggatgagga ttaatatcaa atatcaaaac tcaaatttca
     1141 attgaattcc aaagcccaaa tgtaaaaaaa cctttgaaat ttgggctttg gatttgattt
     1201 gacatttgga ttttgtaatt tgaaatttaa tggcttatcc tcatatccct ctcattcctt
     1261 ctcattcctg ctcattcccc tgctttcatt tgattccagt cttctttatc gattccagct
     1321 tgttttaaga tttcgtttag taagcccgga ctaatatcgc cggaatgttt attttttgcc
     1381 cagaataccc tgcggcttgc cgcagggatg aatgggcgca aaaaataaat cccgccaacg
     1441 gcgggatcaa cagccgcata ccgtcccgcc ttggcgggac ggtgcggttg gtgatttgga
     1501 atatgaacct ttaaatgtcc ttttttgatg aataaatgtt taccgccgct ataaggacct
     1561 tcaaaaccaa ggtgtctgaa attttgaata agcttccgcc aggaaatatt ctttaacata
     1621 attaagctgg catggctttt attttttgat taattttaat tggcaggccg ggaatgctgt
     1681 c
//
LOCUS       DASWHZ010000081         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3143386, whole genome shotgun sequence.
ACCESSION   DASWHZ010000081 DASWHZ010000000
VERSION     DASWHZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143386"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="VGA49_01820"
     CDS             complement(<1..1790)
                     /locus_tag="VGA49_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX9664539.1"
                     /translation="MVEIGPISSISAKTYYFFKNAAPFNNVQVSFCVWLSANKSESKQ
                     ILANLKFWIKGLGLDQEVYLLDFDQPDFIRVLCELDKQNGVFIMPFFYLAQVKIFLTA
                     FREQLIELRAGQVLSPRELKEKLIDRGYESSREELPGTFKHRGSLLTIYSPNYDRPVK
                     VEFDEEKIIRINKEIIEIFPVDLDLNKIARMAKEPIKSNLLKNARFIYADPSLLKREE
                     GWPAVNDQIKDNHQIIFQTLKSKNVLQINFKKPKFYNKNLKKFVVDLKSYLDNNWQVG
                     IAVLEAEVIKNILKSNNLVSDNIKFFDYSVDLEGFEDLDSQYLFLTHQDIFGRIDLKK
                     KIKSGRGARLRMNPETGVWVADLKPGDYIVHQDHGIGLYQGLVKTEIDGFQRENLLLE
                     YAQGDKLYTPIDLAYKVDKYIGQAKPTIYRLSGTSWQQLTQKIKADAEKLAKELLNLY
                     AQREVIKISPWQKIQPEERDLAQAFAYQETTDQQTAIKQIFSDLERDKPMDRLIVGDV
                     GFGKTEVAIRAAFKAVMNNQQVAVLCPTTILAQQHYDTFKDRLKKFPVEIEALSRFTK
                     RLSGIFHEKKVLAGLEKGIVDVVIGTHRLLS"
     gene            complement(2030..2431)
                     /locus_tag="VGA49_01825"
     CDS             complement(2030..2431)
                     /locus_tag="VGA49_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9664540.1"
                     /translation="MADHQYNKDQLDFLMSCLGSNGSSTLNRLSGQRFGFGRNRSDWN
                     KARKKNAWLACLNFRMVDLARRDLSGLDFEEGRFDGADLSGTDFTNASLAGADLTGAI
                     INNQTNFRGADLRGAKVDHSNGGNFEGAIFE"
     gene            complement(2488..2904)
                     /locus_tag="VGA49_01830"
     CDS             complement(2488..2904)
                     /locus_tag="VGA49_01830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX9664541.1"
                     /translation="MKYKSLKVFTDGGARGNPGSAGVGVVVWRGSEVVGQFKKYIGET
                     TNNQAEYQAVILALSKIEDFKPEEVDFYLDSELVVKQLNREYKVRDADLAPLFVKVWN
                     ATVNLKKVTFNHIRREKNKEADKLVNQAIDEHLKKK"
     gene            complement(3054..>4227)
                     /locus_tag="VGA49_01835"
     CDS             complement(3054..>4227)
                     /locus_tag="VGA49_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX9664542.1"
                     /translation="ETDNYPFKYLAGSGVAFKLVQGLLKRKKDADDSLDWEAFEKWLL
                     DLVALGTVADLMPVLGENRTLIKYGLVVLNKTKRVGLKKLLELAGLNYNSLLNTYNLA
                     FQIGPRINAAGRVDHASTAYQLLLTDNQSEANQLADQLEKNNKDRQVLTERIINEAKQ
                     QIEPFNKEQKLIFVYGEDWPLGVVGLVAGRLSQEYHRPAFALGQSQGQISGSGRSIPE
                     FDCIESLEKINHLFARYGGHAAACGFTLAKNDELARLKTELSQIAEKELAGKDLKPRL
                     VIDAEIKFSSVNWDLIEELSKFEPYGQKNPRPVFASYQVKVVGLETVGQDNQHLKLIL
                     TQDNGIVRKAIGFCFGDWCSKIKAGDLIDIVYSVEINEWNGNREIQLKLEDLKLSH"
BASE COUNT         1190 a          963 c          766 g         1308 t
ORIGIN      
        1 gacagcaggc ggtgggtgcc aatcaccacg tcaactatcc ccttttccag gccggctaaa
       61 actttctttt catgaaaaat tcctgataat cttttagtga accggcttaa cgcttcaatt
      121 tcaaccggaa acttttttaa tcggtcttta aaggtatcat aatgctgttg ggccagaata
      181 gtcgttgggc ataaaacagc cacctgctga ttgttcatga ccgctttaaa agccgcccga
      241 atagccactt cggttttgcc aaaaccaaca tcgccgacaa tcagccgatc cattggtttg
      301 tcccgttcaa gatcgctaaa tatctgttta atggctgttt gctgatcagt cgtttcctgg
      361 taggcaaagg cttgggctaa atctctttct tccggctgaa ttttttgcca gggactgatt
      421 ttaatcactt ctctttgggc gtataaatta agcaattctt tggccaattt ttcagcgtca
      481 gctttgatct tttgagtgag ctgctgccag gatgtcccgg ataagcgata aatcgtaggt
      541 ttagcctgac caatatattt atcaacttta taagccagat caattggcgt gtacaatttg
      601 tcgccttgag cgtactccag cagtaaattc tcccgttgaa agccgtctat ttcagtttta
      661 accagtccct gataaagccc aataccatga tcctgatgaa caatataatc gccgggcttt
      721 aaatcagcaa cccagacacc agtctccgga ttcattcgga gtctggcgcc tctgccggac
      781 ttgatttttt tctttaaatc aatccgccca aaaatgtcct ggtgagttaa gaataaatat
      841 tggctgtcta aatcttcaaa tccttctaaa tcaaccgaat aatcaaagaa tttaatgttg
      901 tcagaaacca agttattaga ttttaaaata tttttgatta cttccgcctc caaaacagcg
      961 attccaacct gccagttatt atctaaatag cttttaagat caacaacgaa tttttttaaa
     1021 tttttattat aaaatttagg ttttttaaag tttatctgaa ggacattttt gcttttaaga
     1081 gtttgaaaaa taatttgatg gttatcttta atctgatcgt taacagccgg ccagccctct
     1141 tctcttttta aaagcgacgg gtcggcgtaa ataaaacggg cgtttttcaa cagattagac
     1201 ttaattggtt ctttagccat ccgggcaatt ttatttaaat caagatcaac gggaaaaatt
     1261 tcaataatct ccttattgat tcttataatt ttctcttcat caaattcaac tttaaccggc
     1321 cggtcatagt tggggctata aattgtcagt aaggaaccgc gatgtttaaa tgttcccggc
     1381 agttcttccc ggcttgattc ataaccacga tcaattaatt tttcttttaa ctcccggggt
     1441 gacaatacct ggccagcccg caattcaatt agttgttccc tgaaagccgt taaaaaaatc
     1501 tttacttgtg ccaaataaaa aaaaggcatt ataaaaacgc cattctgttt gtccagttcg
     1561 cataaaaccc tgataaaatc gggctgatca aaatctaaaa gataaacctc ctgatctaag
     1621 cctaaaccct taatccaaaa ttttagattc gccaatattt gttttgattc gcttttatta
     1681 gcgctcaacc aaacacaaaa actcacctga acgttattaa agggggcggc atttttaaaa
     1741 aaataataag ttttggcgga aattgagctg atcggcccaa tttcaaccat ctttttatta
     1801 aaatttactg ccatctccaa atactagcaa taattcagag aaaatccaag tctaatttag
     1861 aggtgataag agggaataag aaggaatcag agggaataag aaggaatcag agggagtaag
     1921 aggggctgtt ctaattaatt attccttata aatttgtata attagtacgc attagcagat
     1981 tagaatcgcg aattggtaac ataaaaaaag cagaccgtga tggtccgctc tattcaaaga
     2041 tcgctccctc aaagttccct ccgttgctat gatcaacttt ggctcctcgc aggtcagctc
     2101 ccctgaaatt cgtctgatta ttgatgatcg ctcctgttag atcggctccg gccaggctgg
     2161 cgttggtaaa atccgtcccg ctgagatcgg cgccgtcaaa gcgtccttct tcaaagtcca
     2221 agccggacaa gtcgcgacga gccagatcga ccattcgaaa attcaaacaa gccaaccagg
     2281 catttttctt tcgggcttta ttccaatctg aacggtttct tccaaagcca aagcgctgac
     2341 cgctcaaacg gttcagggta gatgatccgt tagagcctaa acagctcatt agaaaatcaa
     2401 gctgatcttt attgtattga tggtcagcca tcaatcctcc ccctccttgt tgtcaaggtg
     2461 cgtagttgct acttgtagta aataaattta tttctttttc agatgctcat caatcgcctg
     2521 attaacgagc ttgtcagcct ctttattttt ttctcgtctg atatgattaa atgtaacttt
     2581 ttttaaatta acggtggcgt tccagacttt cacgaataaa ggcgctaaat cagcgtctct
     2641 gactttatat tcccggttta actgtttaac gaccagttcg ctgtccagat aaaagtcaac
     2701 ttcttccggc ttaaaatcct ctatcttact caaagccaat ataacggcct gatattcagc
     2761 ctgattgttg gtggtttcgc caatatattt tttaaattgc ccgactactt cactgccgcg
     2821 ccagacaacc acgcccacgc ccgccgatcc gggattgccc cgggccccgc cgtcggtgaa
     2881 aacttttaaa ctcttatact tcatttttat tttaattatt ttattgttca attacaaatt
     2941 aaggattagg tattaacttt gtaaattcaa gttttaagat ttaagttgtc agccagaggc
     3001 tgatccacct ttggtggata agatttaagt aacttaaatc tgtaatctta aatctaatga
     3061 gatagcttca aatcttctag ttttagttga atctcccggt tgccgttcca ctcgttaatt
     3121 tccacggaat aaacaatatc aatcaaatca cccgccttta ttttactaca ccagtcgcca
     3181 aaacaaaacc cgatcgcctt gcggacaatt ccgttatcct gggtcaggat cagctttaaa
     3241 tgctggttgt cctgaccgac cgtttccagc ccgacaacct tgacctgata tgaagcaaag
     3301 accggccggg gatttttctg tccataaggc tcaaattttg acagctcttc aattaaatcc
     3361 caattaacag agctgaattt aatttccgcg tcaataacca acctgggctt taaatctttt
     3421 ccagccagtt ctttttcggc tatttgagat aactccgttt ttaaccgggc taattcatca
     3481 tttttagcca aggtaaagcc acaggcggcg gcgtgaccgc cgtagcgggc gaataaatga
     3541 tttatctttt ccaaagattc aatacaatca aattcaggaa tgctccggcc tgatccggaa
     3601 atttgcccct ggctttgccc caaagcaaaa gccggccggt gatattcctg cgacaagcgg
     3661 ccggccacca gcccaactac tcccagcggc cagtcttcac cataaacgaa aattaacttt
     3721 tgttctttat taaaaggttc aatttgttgt ttagcctcat taattattct ctcggttaaa
     3781 acttgccggt ctttattgtt tttttcaagc tgatcggcca gctgattggc ttcggactga
     3841 ttatcagtta ataacagctg gtaagcggtg ctggcgtggt caacccggcc ggcggcgttt
     3901 attctggggc caatttgaaa agccaaatta tatgtgttca gcaaactgtt ataattaagc
     3961 ccggccaatt ccaaaagttt ttttaaaccg acccgtttag ttttattaag aacaaccaaa
     4021 ccgtatttaa ttaaagtcct gttttcaccc aaaaccggca ttaaatcagc gactgttccc
     4081 agcgccacca gatccagcag ccatttctca aaagcttccc aatcaagact gtcatcagcg
     4141 tctttttttc tttttagcaa gccctgaacc agtttaaagg ccacgcccga cccggccaaa
     4201 tatttaaaag ggtaattatc cgtttcc
//
LOCUS       DASWHZ010000082         6332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3148676, whole genome shotgun sequence.
ACCESSION   DASWHZ010000082 DASWHZ010000000
VERSION     DASWHZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148676"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VGA49_01840"
     CDS             <1..1253
                     /locus_tag="VGA49_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX9664543.1"
                     /translation="TEVSLITDLDKKIKGISVTEVVTAIISLALQIEASDIHIEAEEN
                     DVKVRYRVDGILNDATVLPKETWPKITSRIKLLAGLKINIENKPQDGRFTIFLSKDQV
                     DVRVSVIPTTYGESIVMRLLRSTAVGLKFEDLGITGRAFADIENEIKKPNGMIITTGP
                     TGSGKTTTLYAVLTRLNTTATKIITLEDPIEYKLEGINQSQVDRSKGYTFAEGLRSIL
                     RQDPDIVMVGEIRDLETADVAINAALTGHLVISTLHTNSAAAGIPRFMAMGVKPFLLA
                     PALNAIVGQRLVRKICPACREEVELDTKTRGEVLKILEKIPPETAAQILTEELHQLKF
                     YKGGGCVECHGIGYKGRIGIYEIMIMVKEIEDLILAGQVSEFKMQEVAVKNGMVTMVQ
                     DGLIKASRATTTVDEVFRVAKTIG"
     gene            1476..1694
                     /locus_tag="VGA49_01845"
     CDS             1476..1694
                     /locus_tag="VGA49_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664544.1"
                     /translation="MAKRKTGAYPHLGEVWTAVDRLGIKTSGEYHRRYQEDPRLYSDP
                     GRHYPDWQGWSAFFGRNNRHGKRRKKTE"
     gene            complement(1748..2827)
                     /gene="tsaD"
                     /locus_tag="VGA49_01850"
     CDS             complement(1748..2827)
                     /gene="tsaD"
                     /locus_tag="VGA49_01850"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX9664545.1"
                     /translation="MLILAIETSCDETSASLLDINSKKINVLSHIVSSQSNIHQKYGG
                     VVPEVAARSHVLRIIPTVQQALGRQKKLDLIVVTAGPGLITSLLVGVQTALGLGLAWD
                     VPVLGVNHLEGHILSNWLTPTGKTPNIKFPALCLIVSGGHTLLVLMKKIGDYKIIGQT
                     RDDAAGEAFDKIAKILGLKYPGGPIISQLARQGNPDSFKMPRPMLHDKNFDFSFSGLK
                     TSVLYKVRDLALKKQIIKKRTKPLNKSKKKGQIVLNKKTVYDLCASFQEAVVDVLTAK
                     TIKAAGQYKVKTIMLSGGVSANNLLRERLRQLIKDQLPKTDYLIPDQKYTGDNATMIG
                     VAGYYNWQTNKQKSLKIKVNPNLKL"
     gene            complement(2958..3170)
                     /locus_tag="VGA49_01855"
     CDS             complement(2958..3170)
                     /locus_tag="VGA49_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664546.1"
                     /translation="MQLQNLADRLNEIRDEYFQGVDIPLSAILQTLQKEIGLKATAAL
                     FDFPTVESFERFMDLILNDSAVEVEA"
     gene            3169..3522
                     /locus_tag="VGA49_01860"
     CDS             3169..3522
                     /locus_tag="VGA49_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664547.1"
                     /translation="MVPPWLVSLRFLGWCQWQNDLIRAVIQLRQIVIRDRCLGERCFD
                     SRAFGQFFCQITGFESDLAQTVARQLDRANGFFDIGFLGFGLVAGFGAARCETQTFEA
                     VGFFFPVQDTCLPSG"
     gene            complement(3645..4376)
                     /locus_tag="VGA49_01865"
     CDS             complement(3645..4376)
                     /locus_tag="VGA49_01865"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEX9664548.1"
                     /translation="MRKILLLLLIAVLALTLSVSLTWAQETTPDSTRVINLAEETLPD
                     HGILPDSPFYFLKRLGEVFGDWLTFDKARQAQRFLDQAETRLAEAKALTDKGNIAKAE
                     TVAQNYEKFLDKASSLADKFQNIENQNEKLEEIIQRIELATSRHRAVLGRVLEQVPDE
                     AKEAIEQAIEVSSQGAQKALEVRQLFRERLENTDREKIRQRLIEERQTIREKLEKIGE
                     KDNDDQETDNEEENNEAEETDDNNG"
     gene            complement(4541..5179)
                     /locus_tag="VGA49_01870"
     CDS             complement(4541..5179)
                     /locus_tag="VGA49_01870"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9664549.1"
                     /translation="MSPKSNLTSIYLVRHGDVLNPAKIIYGNLPLTLSSKGQEQAGKL
                     GKYFAKKNISAIYTSPITRCRQTAALIADKLNLKIKQSQLLTESDWGVFWQGTPIDRM
                     YQKFGADWHTYKTAPGELNYQGVTLETIAKRMNRFIKGAAKKHPGENIICVSHGDPVK
                     ALILELTCQSFNKLRKIKFYYGGVFVLKFSGSRFLEARLLQKGKGYLVNETG"
     gene            complement(5248..>6332)
                     /locus_tag="VGA49_01875"
     CDS             complement(5248..>6332)
                     /locus_tag="VGA49_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9664550.1"
                     /translation="GEKQIFKNRYIIPVIIFLAGATISIFTSPAVRDAAGIWKAYFIE
                     PILLMIVLINSFKEKKDYRLILYALGITTIGFGLLGLFQKITGWGIYEPIWQPADARR
                     ITLTFTSPNAVGLYLGPIIVLYASWFFYQYKDWPKSLFKLAVLILGIVTVLLAVSRGA
                     MLGVIAGLVVVLFFNWSKKGTTLIIAALLSSLFLIKSTAVKLWSFLTFQFPSGELRLY
                     LWKGAKALLLDNWLFGAGLGGFGQLFENYRPENYTEILIYPHNIFLNFWTETGLLGLL
                     GFIAIIFTFFTIAFTINKRQPIDLENKLINIGLLGAMISILVHGLVDVPYFKNDLAVL
                     FWILIALNIIIFNQKINNTGTKQLKN"
BASE COUNT         1689 a         1337 c         1466 g         1840 t
ORIGIN      
        1 aaacagaagt ttccctgatt accgatcttg ataaaaaaat taaaggaatt tcggtgaccg
       61 aagtggtcac ggccattatt tccctggctt tgcagattga ggcttccgac attcatattg
      121 aagccgagga aaatgacgtc aaagtccgct accgggtgga tggtatttta aacgacgcca
      181 ctgtcctgcc caaagaaacc tggccaaaaa ttacttcccg gattaagctc ctggccggtc
      241 tgaagattaa tattgaaaac aaaccccagg acggccggtt cacgattttt ctttccaaag
      301 accaggttga tgtccgggtg tcggttattc caaccactta cggcgaaagc attgtcatgc
      361 ggctcctgcg gtcaacggcg gttggcctga aatttgagga tctgggtatt accggccggg
      421 cttttgctga tattgaaaat gaaattaaaa aacccaacgg gatgattatt acgaccggac
      481 cgaccggctc cggtaaaact accactcttt acgcggtttt aaccaggctc aacaccacgg
      541 ctactaaaat tatcactttg gaagatccga ttgagtacaa acttgaaggc attaatcaga
      601 gccaggttga ccggtccaaa ggctatactt ttgccgaagg cctccggtca attttgcggc
      661 aggatcctga cattgtgatg gttggtgaaa ttcgcgatct ggaaacagcc gacgtggcca
      721 ttaacgccgc gttaaccggg catctggtga tttccactct gcacaccaac agcgccgccg
      781 ccggcattcc caggtttatg gcgatgggcg tcaagccgtt tttactggcc ccggctttga
      841 acgccatagt cggtcagcgg ctggtcagaa aaatctgccc ggcctgccgg gaagaagttg
      901 agctggacac taaaacccgg ggtgaagttt taaagatttt ggaaaaaatt cccccggaaa
      961 cagccgccca aattctgacg gaggaactgc atcagcttaa attttacaaa ggcggcggct
     1021 gcgtggaatg ccacggcatt ggttacaaag gccggattgg gatttatgaa attatgatca
     1081 tggtcaaaga aattgaagat ttgattttgg ccggccaggt gtcagaattt aaaatgcagg
     1141 aagtggccgt taaaaacggc atggtcacca tggttcagga cggcctgatt aaggcgtcgc
     1201 gggcgaccac gacggttgat gaagtgttta gagtggccaa gacgattggg taagatctta
     1261 aatgtcaaaa tccaaagtgt ttcaattcaa atccaaagct caaataacaa aaaatttgac
     1321 atttagattt tggatttcaa ttgatatttg atctttgaaa tttgatattt tatgagtggc
     1381 tccaagtcgg ggtcgttttt ttattatttg actttaaatc acaattaagc taacttattc
     1441 aatgagttct ttcagccggg aatgaaagga tagaaatggc taaacgtaaa acaggcgcct
     1501 atcctcattt gggagaagtc tggacagctg ttgacaggtt agggataaaa acatccgggg
     1561 agtatcatcg gcggtatcag gaagacccgc gactttattc tgatccggga cgccattatc
     1621 ccgattggca gggttggtct gctttttttg gcagaaataa ccgccacggg aagaggagga
     1681 agaagacaga ataaattcca ctttaacgag tggatttttt ttaaaaaaat taaactgttt
     1741 ctgggattta taattttagg ttagggttca cttttatttt taagcttttc tgtttgtttg
     1801 tctgccaatt ataatatccc gccacgccaa tcatcgtggc gttatccccg gtatattttt
     1861 ggtcgggaat taaataatca gttttgggca gttgatcttt gatcagctgg cgcagtcttt
     1921 cccgtaaaag attattagct gatactccgc ccgacagcat gatagtttta actttatatt
     1981 gcccggccgc tttaattgtt ttagcggtca ggacatcaac tacggcttcc tgaaacgaag
     2041 cgcataaatc gtaaacagtt tttttgttta aaactatttg tcctttcttt ttggatttat
     2101 taagaggctt ggttcttttt ttaataattt gtttctttag ggccaggtcg cggaccttgt
     2161 atagaacgga agttttaagt cctgaaaaag aaaaatcaaa atttttgtca tggagcatcg
     2221 gccggggcat tttaaaacta tccggatttc cctgcctggc cagctgactg ataatcggtc
     2281 cgccgggata tttcaggccg agaattttgg cgatcttatc aaaagcctca ccagcggcgt
     2341 cgtctctggt ttggccgata attttatagt cgccaatttt cttcattaaa accaataaag
     2401 tatggccgcc ggaaactatt aagcacaaag ccggaaattt gatattggga gttttaccag
     2461 ttggcgtcag ccagttggat aaaatatgac cttccagatg gttaacgccc aagaccggga
     2521 catcccaggc cagccccagc cctaaagcgg tttgaacgcc gactaataag gaagttatca
     2581 ggcccgggcc ggcggtcaca acaatcaaat ctaatttttt ttgtctgcca agagcttgtt
     2641 gaacagtcgg aataattctt aaaacatggc tccgggcggc cacttccgga acgacgccgc
     2701 cgtatttttg gtgaatgttg ctctgcgaag agacaatgtg ggacaagaca ttaatttttt
     2761 tggaattaat atccagcaaa ctggcgctgg tttcatcgca ggaagtttca atggctaata
     2821 taagcatatc aatcactaat cataagctaa ttaaagcgaa ttcgctttta ttcgcttaat
     2881 gttagcacat tattcgctta gcataaaaag aaggctagct gaaaaaaaat caactagcct
     2941 gtggttggcc gtgacgtcta ggcctcgact tcgactgccg agtcgttcag gatcagatcc
     3001 atgaatcttt caaagctctc gaccgttgga aagtcaaaga gcgcggcggt ggctttaagg
     3061 ccgatttctt tctggagcgt ttgcaggatg gcgcttagtg ggatgtcgac accttgaaaa
     3121 tactcatccc gaatctcatt cagccggtcg gccagattct ggagttgcat ggtcccccct
     3181 tggttggtta gtcttcggtt cctgggctgg tgtcagtggc agaacgacct gatcagggct
     3241 gtgatccagc tcaggcagat cgtcatcagg gatcgctgtc ttggtgaacg ctgttttgac
     3301 agccgggcct tcgggcagtt tttctgccag atcaccggct ttgaatctga tctggctcaa
     3361 accgtcgcca ggcaactgga ccgggctaac ggcttttttg acatcggttt tctcgggttc
     3421 gggctggtgg ctggttttgg agcggcccga tgtgaaactc agacctttga ggcggttgga
     3481 tttttcttcc cggttcaaga cacttgtctc ccctctgggt agcggtgaaa aggaacggaa
     3541 tatcgtcagt cccggctaac gatatttgat aatagcaaag gtttaaaaaa tatacaagcc
     3601 cgtcactttt catgatgggc tttagattgt aaatttagga ttgtttagcc gttattatcg
     3661 tcagtttcct ccgcttcatt attttcttct tcattgtccg tttcctgatc gtcattatct
     3721 ttttctccga ttttttccag cttttctctg attgtttgtc tttcttcaat taatcgttgt
     3781 ctaatttttt cccggtcagt attttccagc cgttcccgga atagctgtct gacttccagg
     3841 gctttttggg cgccctggga ggaaacttca attgcttgct caatagcttc tttggcttca
     3901 tccggcacct gctccaggac tcttcccagg acagcccggt gccgtgaagt ggccaattct
     3961 atcctctgaa taatttcttc cagtttttca ttttgatttt ctatattttg gaatttatca
     4021 gccagcgagc tggccttatc caaaaatttt tcgtaatttt gggcgactgt ttcagctttg
     4081 gcaatattgc ctttgtcagt cagagcttta gcttcagcca gtctggtttc ggcctgatct
     4141 aaaaatcttt gggcctgtct ggctttgtca aaagtcagcc agtcgccgaa gacctctccc
     4201 agtcttttta aaaagtagaa aggcgagtcg ggcagaatgc cgtgatccgg caaggtttct
     4261 tctgccaggt tgataacccg ggtactgtca ggcgttgttt cctgcgccca ggtcaggctg
     4321 actgataaag tcagggccag aacggcgatt aatagcaaga gtaaaatttt tctcataaaa
     4381 ttacattaaa tcttaattgt taaatgttaa atcaaaagtt caaagtacaa agtgcaaagt
     4441 aagtccaaag tttcagccaa aggctgcacg ccatggcggc atgccgtgga tccgtctccg
     4501 gcggacaaag tacaaagtcc aaagcccctg atctcccttg ttatcccgtt tcattcacca
     4561 gatagccttt gcctttttga agcagtctgg cttccagaaa tctgcttcca ctaaatttta
     4621 acacaaagac tccgccgtag taaaatttaa tttttcttag tttattaaaa gattgacagg
     4681 tcagttccag gatcaaagct ttgactggat cgccgtggct gacgcaaatt atgttttcac
     4741 ccggatgttt tttggcggct ccttttataa accggttcat tcttttggcg attgtctcta
     4801 aagtaacgcc ttgataatta agttcaccgg gcgcggtttt ataagtgtgc cagtcagctc
     4861 cgaatttttg atacatccga tcaattggcg ttccctgcca aaaaacgccc cagtcagatt
     4921 cagtcagcag ctgggattgt ttgattttta aatttaattt atcggcaatc aaagccgcgg
     4981 tttgccggca gcgggtaatc gggctggtgt aaatggcgga gatatttttt ttggcgaaat
     5041 atttgccaag ttttccggcc tgttcctggc cttttgagct taaagtcaaa ggcagattgc
     5101 cgtagatgat tttcgcgggg tttaaaacat cgccgtgacg gacgagatag atgctggtta
     5161 ggtttgattt aggggacatt tattttttaa tagagaaaca aaggtgctaa ggtgctaaag
     5221 tgctaaagtg ctaaaaacaa cagttgctca gttttttagt tgcttagttc ccgtgttatt
     5281 tattttttga ttaaagatga ttatgtttaa ggcgatcaga atccaaaaga gaacggccag
     5341 atcattttta aaatatggaa catcaaccag gccgtggacc agaattgaaa tcattgctcc
     5401 cagcaggccg atattgatta atttattttc cagatcaatc ggctgtcttt tatttattgt
     5461 aaaggcaatt gtaaaaaatg taaaaataat ggcgataaaa ccaagcaggc cgagcaggcc
     5521 ggtttccgtc cagaagttta aaaatatgtt atgaggataa attaaaattt ccgtgtagtt
     5581 ttccgggcgg tagttttcaa aaagttggcc aaagccgccc agtccggcgc caaaaagcca
     5641 gttgtccaga agcaaagctt tggcgccttt ccataaataa agtcttaatt cgccggatgg
     5701 aaactgaaaa gttaaaaaag accagagttt gacagcggtt gatttgatta aaaataatga
     5761 gcttagcagg gcggcgatga tcagggttgt cccttttttt gaccagttaa aaaacaagac
     5821 aacaaccagg ccggcgatga cgcccagcat cgctccccgg gaaacagcca agaggacggt
     5881 tacaatcccc agaatcaaaa ccgccagttt aaagagtgat tttggccagt ctttatattg
     5941 ataaaaaaac cagctggcgt aaagaacgat gattggtcct aaatataaac caacagcatt
     6001 aggcgaagta aaagttaaag taatccttcg ggcatcagcc ggctgccaga ttggttcata
     6061 aatcccccag ccggttattt tttgaaaaag acccaaaagt ccaaagccga tagtcgtaat
     6121 gcccaaagcg tagagaatca agcggtaatc ctttttttct ttaaaagaat ttattaatac
     6181 aatcattaat aagattggtt caataaaata ggccttccat attcccgccg cgtcgcggac
     6241 ggcgggtgag gtgaagattg aaatagtagc gccggctaaa aagatgataa ctggaataat
     6301 atatctgttt ttaaagattt gtttttcgcc ct
//
LOCUS       DASWHZ010000083         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_913531, whole genome shotgun sequence.
ACCESSION   DASWHZ010000083 DASWHZ010000000
VERSION     DASWHZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913531"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGA49_01880"
     CDS             <1..449
                     /locus_tag="VGA49_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9664551.1"
                     /translation="SGETLVNIITYCLNPSHYHLLLEQIVDGGITLFMRKLGTGYTNY
                     FNLKHERSGHLFQGAFKAKHVTSNDYFLWLSAYINGNPEIHQTEKAEKYLWSSYREYL
                     GRDDSGICDSQLILDQFKDINDYKKFVKMAIKKSVKIRQEPEEYFL"
     gene            457..975
                     /locus_tag="VGA49_01885"
     CDS             457..975
                     /locus_tag="VGA49_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9664552.1"
                     /translation="MRLLKEIFEKDLDLNGLVPGNPFAEFQRRKTARSVVVDADNKIA
                     LLHFANHNFHLLPGGGVQDNEDLVTALKREIKEETGCEIEIKAEIGKVIERRDAKNQY
                     QESYCYLAAVNGEKCQPRFTDKEIKSGAQVVWIDIDQAIELIEREKPQVYVGYFIQAR
                     ALEFLKKARSLL"
     gene            1021..>1504
                     /locus_tag="VGA49_01890"
     CDS             1021..>1504
                     /locus_tag="VGA49_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664553.1"
                     /translation="MAENISANIDRLLKGFIDAKKTEFVPREATKVKVDKIVSKLALL
                     YEKLRNVIDYNEERLIRRNAIKRILKRKVLYKILSKNSALPLTEELIRAAYLENDKIT
                     ESKVKLVADSIDKYLTLRDCLAPRFQTKKLKNELTEFILGILACEIEEILVPELREKA
                     L"
BASE COUNT          507 a          256 c          326 g          415 t
ORIGIN      
        1 actccggtga gaccctggtt aacatcatca cctactgctt aaaccccagc cattatcatc
       61 ttctgctcga acaaatagtt gacgggggaa taactttatt catgcgaaag ttgggtacgg
      121 gctatactaa ttattttaac ctcaaacacg aacgatcagg ccacctgttc cagggagctt
      181 tcaaagccaa acatgttacc tccaacgatt attttctatg gctgtcggct tatatcaacg
      241 gcaatcctga aattcaccag acagaaaaag ctgaaaaata tttatggagc agttatcggg
      301 aatatctggg gagagatgat tcaggtatct gcgacagcca gttgatatta gatcaattta
      361 aagacattaa tgactataaa aaatttgtta aaatggccat taagaagtca gtaaaaatca
      421 gacaagagcc ggaggaatat tttttataat aaatttatgc ggctcctgaa agaaattttt
      481 gaaaaagatt tagatttaaa cggtttagtg cccggcaatc cttttgctga atttcaaaga
      541 agaaagactg cccggtcagt cgtggttgat gctgataata aaattgcttt gctccatttt
      601 gccaatcata attttcatct tctgcccggc ggcggcgttc aggataacga agatttggtg
      661 actgccctaa aaagagaaat caaagaagaa accggctgcg aaattgaaat taaagccgag
      721 attgggaaag ttattgagcg ccgggacgct aaaaatcaat accaggaatc ttattgttat
      781 ctggccgcgg ttaacggaga aaaatgtcag ccgcggttta ctgacaagga aattaaaagc
      841 ggtgctcagg tagtttggat tgatattgat caggcaattg aattgatcga gcgggagaaa
      901 ccccaggttt atgttggtta ttttattcag gccagggctt tggaattcct taaaaaagcc
      961 cgaagtttac tataaaagct gaattgtttt aaaacttaaa aaatggtata ttagttaaat
     1021 atggctgaaa atatttctgc caatattgac cgcttactga aagggtttat agacgctaaa
     1081 aaaaccgagt ttgttccaag ggaagcaact aaggttaaag ttgataaaat agtcagcaag
     1141 ctggctttgc tttatgaaaa gctaaggaat gttattgact ataacgagga acggctgatt
     1201 agaaggaacg ccattaagag aattttaaaa agaaaagttc tttacaaaat tttatccaaa
     1261 aacagcgctc tgcccttaac tgaagaatta atccgggcgg cttatctgga aaatgataaa
     1321 attacggaaa gcaaagttaa attggtggct gattcaattg ataaatattt aactctgcgc
     1381 gactgcctgg cgccccggtt ccagactaaa aaattaaaaa atgagctgac tgaatttatt
     1441 ctgggaattt tagcctgcga aatagaagaa attttggtgc cggaactacg ggaaaaggcg
     1501 ttgg
//
LOCUS       DASWHZ010000084         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2768847, whole genome shotgun sequence.
ACCESSION   DASWHZ010000084 DASWHZ010000000
VERSION     DASWHZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768847"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VGA49_01895"
     CDS             <1..909
                     /locus_tag="VGA49_01895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX9664554.1"
                     /translation="LGLSLVDMEVGRFPDGEISVKLKEDIRGSDAFLIQPICSTPTMS
                     VNDSLVELQLMLDCLKRASARRITAVISYMGYARQDRKDGGRVPISAKVLADTIVTAG
                     ANRAIIMDLHSAQIQGFFSVPVDHIYGSKVLLPYFKALEIPQLTIVGPDIGSLKDAWA
                     WVKRLDNSYLGLVEKRRLSPQEVETTFIIGPVEDRNILLVDDMIATGGTIENAVRKLK
                     EKGAGDIYLAAIHPVFCGPAWERLGRLLQEGLVRQIVVTDTIPVGDRIPADFPAERLK
                     ILSVAGMLAEIIKRVNRDESVSSVNA"
     gene            complement(1010..>1835)
                     /gene="ychF"
                     /locus_tag="VGA49_01900"
     CDS             complement(1010..>1835)
                     /gene="ychF"
                     /locus_tag="VGA49_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9664555.1"
                     /translation="NKFLANIREVDAICHVVRTFENSDITHVANKIDPESDIETINLE
                     LIMADLATVGKRLDNLKKETKGNNDPDLNKKIQLLEKIKIALDNGQLTEGLGLTDEEK
                     LLIRDLNLLTIKPVIYALNVAEKDINNPTLPLPKICQSQPDKCVSISAKTEADLADLS
                     LPEAREYLNELGLIRSGLDQLIASAYKLLNLITYLTTGPAETRAWTVVKGAKSPQAAG
                     EIHTDFEKGFIKADVIDWQDLVKAGSESAARDQGLIRLEGKEYVVQDGDVIHFKIG"
BASE COUNT          437 a          420 c          476 g          502 t
ORIGIN      
        1 ctgggactgt cgttagttga catggaggtc ggccgttttc ctgacggcga aatcagcgtt
       61 aagctcaaag aagatatcag gggaagtgac gccttcctga ttcagccgat ttgttctacc
      121 ccgacgatgt cagttaatga cagtttggtt gaactgcagt tgatgctgga ttgcctcaaa
      181 cgagcgtcag ctcggcggat cacggccgtt atttcctata tggggtatgc ccggcaggac
      241 cgcaaagacg gcggtcgggt gccgatttcc gccaaagtgc tagcggacac gattgtgacc
      301 gccggcgcca atcgggcgat tatcatggat cttcactccg cccagattca gggctttttc
      361 agcgtgccgg ttgatcacat ctacggctcc aaagttttac tgccgtattt taaggcgttg
      421 gaaattcccc aactgacgat tgtcgggcct gatatcggca gtctgaagga cgcctgggcc
      481 tgggtcaaac gccttgacaa ttcatacctt gggctggttg aaaaaaggag gctgtcgccc
      541 caggaggtgg agaccacctt tatcatcgga ccagtggaag accgcaatat cctgttggtt
      601 gacgacatga ttgccactgg cgggacaatt gaaaatgccg tccggaagct gaaagaaaaa
      661 ggagccgggg atatttatct ggcggcgatc cacccggttt tttgcggtcc ggcctgggag
      721 cggctgggcc gactgcttca ggaaggtctg gtcagacaga tcgtggtaac tgacacgata
      781 ccagttggag accggattcc ggctgatttt ccggcggagc ggctgaagat tttgtcggtg
      841 gccggcatgc tggctgaaat catcaagcgg gttaaccggg atgagtcagt cagcagcgtt
      901 aacgcctaag aggaaagaaa caaccatgtt tgagggcatg gtttttttaa tgaaactaat
      961 gcgcgatgct aatatgcgaa tgtgtactaa ttatactaat tttaaggaat taaccaattt
     1021 tgaaatgtat tacatcgccg tcctgaacaa catactcttt gccttccagc cggattaagc
     1081 cttgatcgcg ggcggcgctt tcactgccgg ctttgaccag gtcctgccag tcaatgacat
     1141 cagctttaat aaagcctttt tcaaaatcag tgtgaatttc accggccgcc tgaggcgatt
     1201 tagcgccttt gacaactgtc caggctctgg tttcagccgg gccggttgtc aggtaagtga
     1261 ttaaattcag tagtttgtag gccgaggcaa tcagctgatc cagcccgctc ctgatcaaac
     1321 ctaattcatt taaatattct ctggcttctg gcagtgacaa atcagccagg tcagcttctg
     1381 ttttggcgga aatactgacg catttatcag gctgggactg acaaatttta ggcaacggca
     1441 gagtaggatt attgatatcc ttttcagcga cattcagagc gtaaattacc ggcttaattg
     1501 tcagcagatt taaatcgcgg atcaatagtt tttcttcatc ggtcagtccc aggccttcag
     1561 tcaattgacc gttatcaaga gcaattttta ttttttccaa aagctggatt tttttattaa
     1621 gatcaggatc gttattgcct ttggtttctt tctttagatt gtctaaacgt ttgccaactg
     1681 tcgccagatc ggccatgatc agctccagat taatggtttc aatatcgctc tccgggtcaa
     1741 ttttattagc gacatgggtg atgtctgaat tttcaaaagt cctgacgacg tgacagatag
     1801 cgtcaacctc ccggatattg gccaggaact tgttg
//
LOCUS       DASWHZ010000085         3092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_171639, whole genome shotgun sequence.
ACCESSION   DASWHZ010000085 DASWHZ010000000
VERSION     DASWHZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171639"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..229)
                     /locus_tag="VGA49_01905"
     tRNA            complement(143..229)
                     /locus_tag="VGA49_01905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Ser,seq:gga)
     gene            complement(441..1142)
                     /locus_tag="VGA49_01910"
     CDS             complement(441..1142)
                     /locus_tag="VGA49_01910"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9664556.1"
                     /translation="MTKYLSKNNLMDGIIVVQLILVLIIGWQIVDLNKKINVTALGPG
                     QAVETASTEPQPVAQATDFEYQVTDQDHTIGPADAKVTIVEFSDFECPFCGRSYPVVK
                     QILTDYQDQVRFVYKHYPLGFHPLAQPAAEASECAGEQGKFWEYHDQIFENQSLMTSE
                     NLRQWAKDLGLNSSQFNRCLDLATYADKVKADLALGNANDVSGTPAFFINNMLVAGSY
                     PYEAMKQVIDQELSK"
     gene            complement(1345..2148)
                     /locus_tag="VGA49_01915"
     CDS             complement(1345..2148)
                     /locus_tag="VGA49_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664557.1"
                     /translation="MTEQQTRFKQIHLLLALILLVVLGVNVFLVTGTSLAVNDRHEEI
                     RPPKLNVTSIIDPDCADCFDLDPFLAYLEEANVEIASRSYEDFGGFGAGVLINQYDIK
                     KIPALIITGEIDKDLNVKALFEDIGEIKFGAFVLTDVRPVYYDLEQEKYVGRFSVTFM
                     DDASCLDCYDVSLHKNALEALEMTPAGQELIDVADDRGQELVEKYKIKFVPTIILGGE
                     LDGYDNFQSIWVTVGTAEEDGAYVFRHGVEQMGKYKVLETGEIIDPFSN"
     gene            complement(2152..2460)
                     /locus_tag="VGA49_01920"
     CDS             complement(2152..2460)
                     /locus_tag="VGA49_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664558.1"
                     /translation="MENFENNPEQNQPERPEQDQQFETILTAAPSDQPDDKRIITGTV
                     RDTIKIAVVVFLIAVSVLEMIQLNKLNKRASADVDSLLYNPKANPIAVANQPQTICGF
                     "
     gene            complement(2584..2826)
                     /locus_tag="VGA49_01925"
     CDS             complement(2584..2826)
                     /locus_tag="VGA49_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX9664559.1"
                     /translation="MAKYKIEFDRQACIGALVCNLLSERFYLKADDGKVDLANAVYNE
                     ATKKWELIIDEADLKANKEAAESCPVDAIKVTKIED"
     gene            complement(2819..>3092)
                     /locus_tag="VGA49_01930"
     CDS             complement(2819..>3092)
                     /locus_tag="VGA49_01930"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX9664560.1"
                     /translation="FYLSLTNLFIFILFLIFYKLNKDRFAGFYVVAYALIYGVARFFL
                     DFLRAADVVGADARYFGLTPAQYASLVFVALGLFLFYRLKYKEVYG"
BASE COUNT          932 a          714 c          584 g          862 t
ORIGIN      
        1 gattcgaact cgcgagggcc tttcgaccct actgcctttc tcccgataat tcatttttca
       61 cggcgcggaa ggggcgagat tcgaactcgc gagggccttt cgaccctact gcctttctcc
      121 cgataattca tttttcacga cgcggaaggg gcgagattcg aactcgcgag ggcctttcga
      181 ccctactgcc tttccaaggc agcgcactag accactatgc gacccttccg cgcctggaaa
      241 aatagaatta tcgaggatcc tcgatccgcc aaaggcggat cgggacaagg cggcgcacta
      301 ggccactatg cgacctctcc atcataaaaa aatttcaaat tataaaatcc aaatgacaaa
      361 tcaaatccaa aaactaaaat ctaaaacttt gacattgaaa ttttggattt caattgaaat
      421 ttgagctttg atatttgaaa ttatttagaa agttcctgat cgatgacctg cttcatcgcc
      481 tcataggggt atgatccggc taccagcata ttattaatga aaaaagccgg tgtgccgctg
      541 acatcgttgg cgttgcctaa agccaggtca gctttgactt tgtcagcgta agtcgccaaa
      601 tccagacagc ggttaaactg gcttgaattt aagcccagat ctttggccca ctgccttaaa
      661 ttttcagaag tcatcaaact ttggttttca aaaatttggt catgatattc ccagaacttg
      721 ccctgttcac cggcgcactc ggaagcttca gccgccggct gggccagtgg atgaaaacca
      781 agaggataat gtttatagac aaatctgacc tgatcctgat agtcagttaa aatctgctta
      841 accaccgggt aggatcggcc gcaaaagggg cattcaaaat cagaaaactc cacaatcgtt
      901 actttggcgt cagccgggcc aatggtatga tcctgatcag tcacctgata ttcaaaatca
      961 gtcgcctgag ccaccggctg gggttcagtt gaggctgttt cgactgcctg accgggaccc
     1021 agggcggtaa catttatttt tttattcaaa tcaacaatct gccagccaat tatcaatacc
     1081 agaattaact ggacaacgat aatgccgtcc attaaattat tcttacttaa atatttggtc
     1141 ataggtattc tatcaaatta taaattataa tatcgaaatc ctaaataaat tctaaaatct
     1201 aatgttcaaa attctaaaca atcaataaaa tttatttgtt tggaacattt gaatttgata
     1261 tttatgattt atttagtatt tctcccgact ttgtcgagga tcctcgatgg ctttgccatc
     1321 gggagaattt tggatttagg ctttttaatt actaaatggg tcaattattt ctcctgtctc
     1381 caaaaccttg tacttcccca tctgctccac gccatgcctg aaaacataag cgccgtcttc
     1441 ttccgcggtg ccgaccgtca cccagattga ctggaaatta tcataaccgt caagctcgcc
     1501 ccccagaata attgtcggca caaatttaat cttatatttt tcaaccagtt cctgcccgcg
     1561 atcatcagcc acatctatta attcctgacc agccggagtc atttccagag cttccagggc
     1621 gtttttatga agggaaacat cataacaatc cagacagctg gcgtcgtcca taaaagttac
     1681 gctaaaccgg ccgacatatt tttcctgttc caggtcataa taaaccggcc ggacatcagt
     1741 taaaacaaaa gctccaaatt tgatttctcc aatatcttca aacaaagctt tgacattcag
     1801 atccttatca atctcgccgg tgataatcag ggccggaatt tttttaatat catattgatt
     1861 aatcaaaacc ccggctccaa aaccgccaaa gtcctcataa ctacggctgg cgatttccac
     1921 attagcttct tccagataag ccagaaatgg atccaggtca aaacagtcgg cacagtcagg
     1981 atcaataatt gaagtaacat taagctttgg gggacgaatt tcttcatggc ggtcattaac
     2041 ggccagtgaa gtcccggtca ctaaaaaaac attaacaccc aggacaacca gcaatattaa
     2101 agccagcaat aaatgaatct gcttaaatcg ggtttgctgt tcagtcataa attaaaagcc
     2161 gcagatcgtc tggggctgat tagcgaccgc gatcgggtta gccttcggat tataaagcag
     2221 gctgtcaaca tcagcgctgg ctcttttatt aagcttattt aactgaatca tttccagcac
     2281 cgacacggca attaaaaaaa caacaaccgc gattttaatg gtatctctga cagttcctgt
     2341 gataatccgc ttatcatcag gctgatcaga cggggcggca gtcaaaatag tttcaaattg
     2401 ctggtcttgc tctggccgtt ctggttggtt ttgctccgga ttattttcaa aattttccat
     2461 aatatataat gttatccgaa tttacgaatt ctatccgaat tgacgaataa caaatatgtt
     2521 gtattcgtga tttgcggatt ggcataagat tagttgatta gcataacatt tgcttatcaa
     2581 aagtcaatcc tcaatttttg taactttaat agcgtcaacc gggcaagatt cagccgcttc
     2641 tttattggcc ttgaggtcag cttcatcaat aatcagctcc cattttttgg ttgcttcgtt
     2701 ataaacagcg ttagccagat caaccttgcc gtcgtcagcc ttcagataaa atctttctga
     2761 cagtaaatta cagactaagg cgccaatgca ggcctgacgg tcaaattcta ttttatactt
     2821 agccataaac ttctttatac tttaatctgt aaaacaaaaa cagacccagg gccacaaaaa
     2881 ccaaactggc gtattgggcc ggggttaaac caaaataacg ggcatcagct ccgacaacat
     2941 cagccgctct caaaaaatcc aaaaagaaac gcgccactcc gtaaatcaag gcgtaagcga
     3001 cgacataaaa accagcaaaa cggtctttgt taagtttata aaaaattaaa aatagaataa
     3061 aaatgaaaag attggtcagg cttaaataaa aa
//
LOCUS       DASWHZ010000086         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2043553, whole genome shotgun sequence.
ACCESSION   DASWHZ010000086 DASWHZ010000000
VERSION     DASWHZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043553"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VGA49_01935"
     CDS             complement(<1..1016)
                     /locus_tag="VGA49_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9664561.1"
                     /translation="MSKSKKNLSYLDDFVMSIELDAKGFISKANRQFLKISKYTEKGI
                     LGKHFNNLIFDAGKDLLGQKIWKIISGGKSWEGEIKNLDRDGSFFWTETKIYPVVEGR
                     ARPIKYLVAQFPITRCKIIEEQMTEESNFMQVLWTAAVSANESLNVEEALRTAVNSVC
                     VLMNWPVGHALFLADDRKEFISSKIWYFDAPAKFADFRNISEQAIYGLGHHLLGTALA
                     TGKVAWSQDVGEYANFAARYSLAKKLDINGSLVSPILVGKEVLGMMEFFAPEIHKPSP
                     GVIKLLTQVGLQIGRVMERKRMEVRLLDSVGDGILAIDRSWNITIFNQAATKISGWKK
                     EEVI"
     gene            complement(1022..1504)
                     /locus_tag="VGA49_01940"
     CDS             complement(1022..1504)
                     /locus_tag="VGA49_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX9664562.1"
                     /translation="MQKTIQIETGELAVGHNDTKIKTGSVGSCVVIVLYDEEARVGGM
                     AHAMLPSRPSNLTVEATGQEKFSATYVEEAVDGLLNQVKSIGGKRARLKAKLVGGATM
                     FKVLSGDKYGIGFQNIEAARRRLSELFIPIVAEDTGGSMGKIVEFNLVNGLVDVSTKL
                     "
     gene            complement(1507..2130)
                     /locus_tag="VGA49_01945"
     CDS             complement(1507..2130)
                     /locus_tag="VGA49_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX9664563.1"
                     /translation="MAKFLSNFERDQLVEVANIGADHASTALSQMVKKRVSIRVPEAY
                     VDRIEKLVKYMGDPKDLVTAVLLKILGDAPGIMLLIFTPASALNLTSLLTKKRQKNPK
                     VLDEIDRSALKELGNILSGASMTALARFLNINLLQSIPDIATDMLGALTDNVLAEIGR
                     ESELALVLKVNFFINEVNIDANLFLLFNSISTDKILDMTRKKIFQSA"
     gene            complement(2123..>2807)
                     /locus_tag="VGA49_01950"
     CDS             complement(2123..>2807)
                     /locus_tag="VGA49_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9664564.1"
                     /translation="EKDVITAEKADELTEKADHDLSREVLELLFHGLSTKETVTETSG
                     RGVGLSVVKNFTDQVGGTVDILSPISPSGGSRFTLELPLTLAIIKALLVKVSDSVFAI
                     PFSSIERIVSIQLSEIKSMADQFVAVVDGANLPLALVGKIFGIDTPAAVAPLMTVVVK
                     KGPERAGLVVDTLLDEQEIIVKPLSSILKGVKGFSGSTILGDGRTILILDVASVLEDT
                     KKLLRVSYG"
BASE COUNT          786 a          691 c          510 g          820 t
ORIGIN      
        1 tttatcactt cttccttttt ccaaccagaa attttcgtcg ccgcctggtt aaaaatcgtg
       61 atgttccagc tcctgtctat cgccaaaata ccatcgccaa cactatcaag cagtctcacc
      121 tccattctct tgcgttccat cactcgtccg atctgtaggc ctacctgggt taataatttg
      181 atgacgccgg gactgggctt gtgaatttct ggagcgaaga actccatcat tccgagaact
      241 tctttgccaa ctaaaattgg cgagaccaga cttccattga tatccaactt tttagctaat
      301 gaatagcgcg cggcaaaatt agcgtattcg ccaacgtcct gggaccaggc tactttgcca
      361 gtggctaagg ccgtacccag caaatggtgc cccaaaccat agattgcctg ttcactaatg
      421 tttcgaaaat ccgcgaattt ggccggagcg tcaaaatacc aaatctttga agagataaat
      481 tctttgcgat cgtcagcaag aaagagcgca tgaccaaccg gccaattcat taaaacgcag
      541 acggagttaa cggccgtccg caaagcttct tcaacattca aagattcatt agctgacaca
      601 gccgctgtcc aaagaacctg cataaaattc gattcttcag tcatttgctc ctcgatgatt
      661 ttgcacctag taattggaaa ctgagcgacc aggtatttaa tcggacgcgc ccggccttcc
      721 acaaccggat aaatttttgt ttctgtccaa aaaaatgaac catccctgtc caaatttttg
      781 atttctcctt cccaactctt tccgcctgaa ataattttcc aaattttttg acctaataaa
      841 tctttcccgg catcaaatat tagattatta aaatgttttc ctaaaattcc cttttcagtg
      901 tacttggata tttttaggaa ttgccggttg gccttactta taaatccttt agcgtcaagc
      961 tctatagaca ttacaaaatc atccaaataa cttaaatttt tttttgattt agacatataa
     1021 attacaactt ggtgcttaca tccactaatc cattgactaa attaaattca acaattttgc
     1081 ccatgcttcc gccggtgtcc tcagccacaa ttggtatgaa cagctcggaa agccggcgtc
     1141 tggcggcttc aatattttga aagccaatgc cgtatttatc gccgcttaaa actttaaaca
     1201 tggtcgcgcc gcccaccagt ttggctttaa gccgcgccct ttttccccca attgacttaa
     1261 cttggtttaa taaaccatca accgcttctt ccacgtatgt agcagaaaat ttttcttggc
     1321 ctgtcgcctc caccgtgaga ttagaaggcc tggacggcag catggcgtga gccatgccac
     1381 caacccgagc ctcctcgtcg taaagtacga tcaccacgca cgagccgaca ctccctgtct
     1441 taattttggt gtcattgtga ccaaccgcca gttcccctgt ttcaatctgg atagtttttt
     1501 gcataattat gctgattgga aaattttttt tctggtcatg tccaaaattt tgtcagttga
     1561 aattgaatta aataaaagaa acagattggc atcaatattt acttcattaa taaaaaaatt
     1621 aacttttaaa accagagcca actcgctttc tctgccaatt tccgccagca cgttatcagt
     1681 cagagcgcct aacatgtctg tggcaatgtc tggtattgat tgcaaaagat ttatattcaa
     1741 aaaacgagcc aaagcagtca tggacgcgcc ggaaaggata ttccctagct ccttgagagc
     1801 cgaccggtcg atctcatcca aaactttagg atttttttgg cgttttttag ttaaaagact
     1861 agtcagatta agggcgctgg ctggagtaaa gattaacaac ataatgccag gcgcgtcacc
     1921 taaaattttt aacagcacgg ccgtgaccaa atctttagga tcacccatgt actttactag
     1981 tttctcaatc cggtcaacat atgcttctgg gacacgaatg ctgacccgtt ttttgaccat
     2041 ttgcgacaag gcggttgaag cgtgatcagc cccgatatta gccacttcaa ccaattgatc
     2101 cctttcaaaa ttagataaaa atttagccat aagaaactct taataatttt tttgtatctt
     2161 caagcaccga tgctacgtct aaaatcagaa ttgtccggcc atcccctaaa atcgttgatc
     2221 cagaaaaacc ttttacgcct tttaatatcg aggacaatgg cttgacgata atttcttgtt
     2281 catcaagaag cgtgtctacc acgagtccag ctctttctgg cccctttttt acgacgacgg
     2341 tcattagagg agcgacggcg gctggagtgt ctatcccaaa aattttcccc accaaagcta
     2401 aaggcaaatt tgctccatca accacggcca cgaactggtc agccatgctt tttatttctg
     2461 aaagttgtat tgaaacaatc ctttcaatac tagaaaacgg tatggcaaaa actgaatcac
     2521 tgactttaac taataaagcc ttaataattg ccagtgttaa aggaagctcc agcgtgaaac
     2581 ggctgccgcc agacggacta atcggagaca agatatccac tgttccgccc acctgatctg
     2641 taaaattttt aacaacgctt aagccgaccc cgcgtcccga cgtttccgtg acagtttctt
     2701 tagttgaaag gccatgaaac aaaagttcta aaacttctcg ggacaggtca tgatctgctt
     2761 tttcagttag ctcgtcggct ttttccgccg tgattacgtc cttttca
//
LOCUS       DASWHZ010000087         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_560055, whole genome shotgun sequence.
ACCESSION   DASWHZ010000087 DASWHZ010000000
VERSION     DASWHZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560055"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1218)
                     /gene="metK"
                     /locus_tag="VGA49_01955"
     CDS             complement(40..1218)
                     /gene="metK"
                     /locus_tag="VGA49_01955"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9664565.1"
                     /translation="MHKHYLTSESVTEGHPDKMADQISDAILDEVMRCDPQGRVAVET
                     LLTNGLAVIAGEMRTACYVDVPQIVRQTIKDIGYTDARFGFDWQTCGVAVAIQEQSVD
                     IALKVDAFKNKKSTAENLASLGAGDQGLMFGYATTETKELMPLPITLAHKLARRLAEV
                     RQKKIIPYLRPDGKSQVTVEYADGRAVRLENVVISAQHHPEVKTDKLRKDILVKVIQK
                     IIPKKLIDKNTKYHINSTGRFVLGGPRADTGLTGRKIIVDTYGGVGSHGGGCFSGKDP
                     TKVDRSGSYAARWVAKNIVAAGLADKCEIQLAYVIGIPEPLSVNVNSFGTGRIADEKI
                     AKLVKKVFDLRVGLIIKELKLRQPIYRQAAAYGHFGRNDLKLPWEQTIKAAQLKKLAL
                     "
     gene            complement(1247..>1974)
                     /locus_tag="VGA49_01960"
     CDS             complement(1247..>1974)
                     /locus_tag="VGA49_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664566.1"
                     /translation="LAEEAEARIALKEQEIQQKLNQIIEKIRNNRGSDPNQKIFVDAK
                     KEHAKLIQEIGEEAFWEQWYKDEAMKIALEQADFQKLYKELKTLMGEAGNYRDLSRRE
                     LFAKKGLLERAKEVNLNQAIHLRLQMEDYYQGLASRLRLGLTPAETKQLIGNNQRQIQ
                     DLGENGERKFLKEWALTKARQILTYDINSADYKRFKSLETMLKNNIKKLREQGKEKPA
                     VIDPLEWDWQIKGIPEISVPPKK"
BASE COUNT          479 a          470 c          416 g          609 t
ORIGIN      
        1 agagggaatg agagggaatg agagggaatg agaggaatac taaagcgcta atttctttaa
       61 ttgggcggct ttgatggttt gctcccacgg taattttaaa tcattccggc caaaatggcc
      121 ataagcggcc gcttgtctgt aaatcggctg tcttaatttt aactctttaa ttatcagacc
      181 aacccgtaaa tcaaaaacct ttttaactaa tttcgctatt ttttcatcag caatccggcc
      241 ggttccaaaa ctattaacat taactgacaa tggttctgga ataccaatca cataagccag
      301 ctggatttcg catttatcgg ctaagcccgc ggccacgata tttttggcga cccagcgggc
      361 ggcgtaactg cccgaccggt caactttggt cgggtctttg ccgctaaaac agccgccgcc
      421 gtgactgccg actccgccgt aagtgtcaac gattattttt cggccggtca agccggtgtc
      481 agcccttggc ccgcccaaaa caaaccggcc ggtggaatta atatgatatt tagtgttttt
      541 atcaatcaat tttttaggga taattttttg aatcactttg accagaatat cttttcttaa
      601 tttgtcagtt ttgacttcag gatgatgctg ggcggaaatg acgacatttt ccagtcggac
      661 agcccggcca tcagcgtact caactgtcac ctgggatttg ccgtccggcc ggagataagg
      721 aataattttt ttctggcgga cttcagccag tcggcgggcc agtttatgag ccagagtaat
      781 tggcaaaggc atcagctctt tggtttcagt tgtagcgtag ccaaacatca gcccctgatc
      841 gccggctccc aggctggcca aattttcagc ggttgacttt ttgtttttaa aagcgtcaac
      901 ctttaaagca atatcaactg attgttcctg aatggcgacg gccactccac aagtttgcca
      961 gtcaaagcca aagcgggcgt cagtgtagcc aatgtcttta attgtttgcc ggacaatctg
     1021 gggaacatca acataacaag cagtcctcat ttcgccggca atgaccgcca gaccattggt
     1081 cagcaaagtt tccaccgcca cccgtccctg gggatcacag cgcatcacct catccaaaat
     1141 agcgtcggaa atctggtcgg ccattttatc cggatggcct tcggtaactg attcagaggt
     1201 gagatagtgt ttgtgcataa attaggggat aggggatagt tattgattat tttttggggg
     1261 ggacggaaat ttcagggatt cctttgatct gccaatccca ttctaatgga tcaattacag
     1321 ccggcttttc tttcccctgt tcccttaatt ttttaatatt atttttgagc attgtctcca
     1381 gactcttaaa tcttttatag tcggcgctgt taatatcata agtgagaatc tgccgcgctt
     1441 tagtcaaggc ccactccttt aaaaactttc tttcaccgtt ttcacccaga tcctgaatct
     1501 ggcgctgatt attgccaatt aactgttttg tttcggccgg agtcagtccc agtcgcaatc
     1561 tgctggctaa accctgataa tagtcttcca tttgcagtcg gagatgaata gcctgattta
     1621 aattaacttc tttggctctt tccaataaac cttttttagc gaacaattct cttcgggaca
     1681 aatcacggta attaccggcc tcgcccatca gcgtttttaa ttctttatac agtttttgaa
     1741 aatcagcctg ctccagagca attttcatgg cttcgtcttt gtaccactgc tcccaaaaag
     1801 cttcctcgcc tatctcttgg ataagtttgg catgctcctt tttggcgtca acaaaaattt
     1861 tctgattagg atcgctgccc cggttatttc taatcttttc aataatctga ttcaattttt
     1921 gctgaatttc ctgctcttta agggctatcc tggcttcagc ctcttcggct aaat
//
LOCUS       DASWHZ010000088         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_924603, whole genome shotgun sequence.
ACCESSION   DASWHZ010000088 DASWHZ010000000
VERSION     DASWHZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924603"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..690
                     /locus_tag="VGA49_01965"
     CDS             163..690
                     /locus_tag="VGA49_01965"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX9664567.1"
                     /translation="MKKIFIIIIALAILVWLIFSLTRKQEQSFVNNFFECINAGNLLM
                     ESYPRQCRTKDGRLFVENIGNELKKLDLIKISNPRPNQVITSPLAIEGQARGYWFFEA
                     DFPVRLFDANDQEIGLAIAQAQGDWMTEDFVPFKAELTFDQPGTDRGYLILEKDNPSG
                     LPENADQLIVPVFFR"
     gene            727..1467
                     /locus_tag="VGA49_01970"
     CDS             727..1467
                     /locus_tag="VGA49_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX9664568.1"
                     /translation="MIAFLWSIGATFVVSLISLISIVFLSFKEKVLEKILLILVGFSA
                     GALLGNAFFHLIPEIVEEGVSQAVFIYIVVGFCLFFILEKFIFWHHCHTYKCEVKTFT
                     YMNLVGDGLHNFIDGLVIAASFVTSIELGLATTLAVLIHEIPQEISDFGVLIYGGFSR
                     GKALLYNFLSALLAIIGAVAGFALVLNIEGVTKFLLAFTAGGFIYIAAADLIPELHKE
                     IKIKKYLLSFVSFLASLVLMYFIKVWFD"
     gene            1578..1763
                     /locus_tag="VGA49_01975"
     CDS             1578..1763
                     /locus_tag="VGA49_01975"
                     /inference="COORDINATES: protein motif:HMM:NF021626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9664569.1"
                     /translation="MREYRCSKDNKLLMKACLIGGDVEIKCRHCGRLNQFKGDDKLNL
                     KYLCYKYPCEHRIYQPE"
     gene            1932..>2760
                     /gene="nrdD"
                     /locus_tag="VGA49_01980"
     CDS             1932..>2760
                     /gene="nrdD"
                     /locus_tag="VGA49_01980"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEX9664570.1"
                     /translation="MTKIKKIRKRDDSIVDFDPEKITAAIAKAMQAVDQLDEELAKKL
                     TNKVLETLYLKFHTRTIPAVEEVQDLVEEVLIKENLIKVAKAYIIYREQHYQMRKIRN
                     VISKDLVDGYLNKADWRVRENSNMSFSLQGLNNHISSSISSQYWLNKVYPNEIRNAHV
                     HGDFHIHDLQLLAAYCCGWDLKDLLIRGFGGVTSKIESRPARHFRSALGQVVNFFYTM
                     QGEVAGAQALSNFDTYLAPFIRYDNLDYDQVKQCLQEFLFNMNVPTRVGFQTPFTNIT
                     "
BASE COUNT          760 a          523 c          608 g          869 t
ORIGIN      
        1 aactgttttt gagtcatagt aaattctagt gtcatacttg aaatggttaa tgattaacca
       61 tttcattata tattaggtat gacattttaa tttccttgta ctatgacatt atcacttccg
      121 tgcgacagct tttgaaattt gatataatga agctaagcaa ctatgaaaaa aatttttatt
      181 ataatcattg ccctggcaat cctggtttgg ttgattttta gtttaaccag aaagcaggaa
      241 cagtcgtttg tcaacaattt ttttgaatgt attaatgccg gcaatctgct gatggaaagc
      301 tatccccggc agtgccggac taaagacggc cggttattcg tggaaaatat tggcaacgaa
      361 ctgaaaaaac ttgatttaat caaaatcagc aatccccggc caaaccaggt tattaccagt
      421 cctctggcaa ttgaaggcca ggcccggggc tactggtttt ttgaggcgga ttttcctgtc
      481 aggctgtttg acgccaatga tcaggaaata gggctggcca ttgcccaggc ccagggcgac
      541 tggatgactg aagactttgt cccctttaaa gctgaattaa catttgatca gccagggact
      601 gaccgcggct atttgatatt agaaaaagac aacccgtctg ggctgcctga aaacgctgat
      661 cagttaatag tgccggtttt tttcagatag tttgctataa tataataaat agtctataaa
      721 caaattatga ttgcattttt atggtcaatt ggagcgacct ttgtcgtcag tctgatttct
      781 ttaatcagca ttgttttttt gtcgtttaag gaaaaagttt tggaaaagat tttattgatt
      841 ctggttggtt tttcggccgg cgctctactg ggcaacgctt tttttcacct gatccctgaa
      901 atagttgaag aaggcgtcag ccaggcggtt tttatttata ttgtagttgg cttctgtctg
      961 ttttttattc tggaaaaatt tattttctgg catcattgtc atacctataa atgcgaagtc
     1021 aaaaccttta cttatatgaa tttggtgggg gacggcctcc ataattttat tgacggcctg
     1081 gtcattgccg ccagttttgt aaccagtatt gagctgggcc tggcgacgac tctggctgtg
     1141 ctgattcacg aaattcccca ggagattagt gattttggag ttttgatcta tggaggtttt
     1201 tccaggggca aagcgttgtt gtataatttt ttatccgctt tattggcgat aatcggcgcg
     1261 gtggccggct ttgctttagt tttaaatatt gaaggcgtta ccaaattttt actggctttc
     1321 actgccggcg gctttattta tatcgcggcg gctgatttga ttcccgaact tcataaagaa
     1381 attaaaataa aaaaatattt attgtctttt gtttcctttt tggccagttt agttttaatg
     1441 tattttatta aggtttggtt tgattaatgt agttttccac atggctgatg gtttttggct
     1501 aaaacatcgg cctttttatt taattgacaa cacattatat agtggtaaaa ttaattacac
     1561 cctactatat atagtatatg cgggaatacc gttgctccaa ggataataag cttttaatga
     1621 aagcctgcct gattggcggc gatgttgaaa taaaatgccg ccactgcggg aggctcaatc
     1681 aattcaaggg agatgataag ctgaatctga agtatctgtg ttataagtat ccctgtgaac
     1741 accggattta tcagcctgaa tagaggttta tacaataaaa taataacaga agcaccagat
     1801 gctcattccc aacaggaatg agttttgttt ttatcagtca acagccaaac agtcaatagt
     1861 caacagggca gggatgaggg atttgtattc cctgttggcc gctgactgtt gactgctgac
     1921 atataatatt tatgaccaaa atcaaaaaaa tcaggaagcg agacgactct attgtagatt
     1981 ttgatccgga aaagatcacc gcggctattg ccaaagcgat gcaggcggtt gaccagctgg
     2041 acgaagagtt ggctaaaaaa ttaactaata aagtcttaga gactctttat ttaaaatttc
     2101 acacccggac aattccggcc gttgaagagg tccaggactt ggtggaggaa gttttaatta
     2161 aagagaatct gatcaaagtc gccaaagctt acattattta ccgggaacag cattatcaga
     2221 tgagaaaaat caggaatgtt atttccaaag acctggttga cggctatctg aataaagccg
     2281 actggcgggt cagggaaaat tccaatatgt cattttctct ccagggtctg aataatcaca
     2341 tttcctccag catcagttcg cagtactggc taaataaagt ttatcccaat gaaatccgca
     2401 acgcccatgt ccacggcgat ttccacatcc atgatttgca attactggcc gcctactgct
     2461 gcggctggga tttaaaagat ttgttaatca gggggtttgg cggggtaacc agcaagattg
     2521 aaagccgtcc ggccaggcat tttcggtcag ccctggggca agtcgtgaat tttttttaca
     2581 caatgcaggg cgaagtcgcc ggcgcccagg ccttgtccaa ttttgacact tatctggcgc
     2641 cgtttatccg ctacgacaat ctggattacg accaggtcaa acaatgtctt caggaatttt
     2701 tgttcaatat gaacgtgccg acccgggttg ggtttcagac gccgtttacc aacattactt
//
LOCUS       DASWHZ010000089         5992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_926792, whole genome shotgun sequence.
ACCESSION   DASWHZ010000089 DASWHZ010000000
VERSION     DASWHZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926792"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VGA49_01985"
     CDS             complement(<1..622)
                     /locus_tag="VGA49_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9664571.1"
                     /translation="MLEKSPVINVIKKTTPAVVSIVISKLFYKLRQELPFDLFGPFGL
                     PFPFLEKIPPKKKIRIGGGSGFIVSSDGIVLSNRHVVIDPQSDYTVVTSDGQEYKAKV
                     LARDSIYDIAVLKIDSKKKFSTIKLGDSSKLQLGQTTIAIGNALGEFQNTVSTGIVSG
                     LSRYITAHDAMAKQSQRLRGLIQTDAAINPGNSGGPLLDIEGKAIGI"
     gene            732..1169
                     /locus_tag="VGA49_01990"
     CDS             732..1169
                     /locus_tag="VGA49_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664572.1"
                     /translation="MLKHLKQILILAIIIVSFGLAGSALAQNVTVNNGDLIKGGSPAV
                     YYYLDGQRFVFPNEKIFKSWFADFTTVKIIPDEQLAAIQIGGNITYRPGARLIKIISN
                     PKVYAVEPGGLLRWVKTEELARLLFGDNWALLVDDLPESFFVN"
     gene            1431..3143
                     /locus_tag="VGA49_01995"
     CDS             1431..3143
                     /locus_tag="VGA49_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664573.1"
                     /translation="MAFGEAADIAPADDIIGNDLLTEKKELAILLGSPAVSRVVQKKP
                     QAEIGIFSFKASQATGYTITYLRLNGFIDQNKGNPDFGLGRDDDRDGSTYLSRILNNI
                     NLYNDQDELIAGPVNMSLDGFAVFNNLTLAVPSDQTIKIIVKADVNTELLAQDTYRFS
                     IDIVSDLDISVVDDNNIKIIPSGLSPNNGLNPYSFLTIQRNGQLTVENGPNNPPTDIV
                     LSGQPDVLIYSLKFTAKYEPFLVQKLGFERISQEANNTIKEISFTYPTQTGQITVSSP
                     FSFNQVKFTNLGFYLPANTDFTLEIKAETYPYQGYYNSGSEIKIKMLYDENFIVKGLE
                     SGVELTYFDDTVGNLFNKTTPSNTLIVRQTKLSARLSPETPESLSSRGFNPVLRFEVT
                     ADPAGPARLRKLTFKVTSSSVNFIGDDNDLFERWADVNGDLLDDDDVAAIYNLNDLNK
                     SLAGGFNGRVEFAIFDHSANTLDTTPQGFQTEANDYGIIKISFASQPLISAGQTETFI
                     LYLNSAYIGDQGATLKAELLGDNQNANFTNNNFEWHDGQMPATGYLIKNLPIGGKLLT
                     FAEQ"
     gene            complement(3350..4039)
                     /locus_tag="VGA49_02000"
     CDS             complement(3350..4039)
                     /locus_tag="VGA49_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664574.1"
                     /translation="MATQVQDLESLCVELNNDLLMLEKGVEGQEKSNRQLSTRVAKFF
                     DMIISVIVSIGKRLDKLEDYDAELGKEVFTGDSYTLLNRVNKLEQDADGQSTATGQPI
                     RVAVVSSAIAVAVSVLLSVLLFGFLLGNDRQQITSLATKVNELTEKAETPQFETATAN
                     QDELNKLKERLTGLEQQVAQLKKESPKGHQKRLQKLEEDVATLGSWIDQLVGLWEKVT
                     SGLEAAHSEKK"
     gene            complement(4333..5445)
                     /locus_tag="VGA49_02005"
     CDS             complement(4333..5445)
                     /locus_tag="VGA49_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664575.1"
                     /translation="MTLVYKPDKVVVSPELFGGGFSMGKGQEPESERDGYRKQLCELI
                     NEMDSFHKELAALQARLTESQGVQEEIGKTVAGIHQALANGPVGGSAELTRLVKAFET
                     AIDRMSLASEKAIERLVANGADEVARAKIEALATEQQRFSESVSQSTTNASIVRQNMD
                     RVKQIADEGRNNKEETASLSHKVDEIAGSLVRQLRRTRIFAMSSFLFLGLLFAILAYA
                     SDQQLADAYYQATGQPELVTEAAVTDQLAGYQQSFQEMTDQLAILEGRQTENQQNQAD
                     SAKQLDQKLENLMQLVEGHGQQINKTLGRVPSDQTLTDWLNKSLAFQSKEDAVASAQK
                     LADSLANLSREVARLQQELAKLQPAKNGEPKKENKK"
     gene            5508..5831
                     /locus_tag="VGA49_02010"
     CDS             5508..5831
                     /locus_tag="VGA49_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664576.1"
                     /translation="MWADDDEDDDWMDDDLNGETEETYSGLSVFSSDYWEAVHQERER
                     DKKTDKDFRLYVWLVLFIIMLFGCLVVSVLANLGLIKPRLVYVILLGLGIGLALRLTG
                     IFREQ"
BASE COUNT         1585 a         1433 c         1303 g         1671 t
ORIGIN      
        1 taatgccgat ggctttaccc tcaatatcca gcaaaggccc gccggaattg cccggattga
       61 tggcggcgtc agtctgaatc aacccccgca aacgctggga ctgtttagcc atggcgtcat
      121 gagcggtgat atatctggac aaaccggaaa caatgccggt agaaacggta ttttgaaatt
      181 cccccagagc gttgccaatg gcaatggtcg tctgtcccag ctgaagcttt gatgaatcac
      241 caagcttaat cgttgaaaac tttttctttg aatcaatttt taaaacggca atgtcataaa
      301 tggagtcccg ggccaaaact tttgctttgt attcctggcc gtcgctggtg acgacagtat
      361 agtctgactg gggatcaatc accacgtggc ggttgcttaa tacaatcccg tcgctggaaa
      421 caataaagcc gctgccgccg ccgatcctga ttttcttttt gggcggaatt ttttccaaaa
      481 aaggaaacgg caagccaaac ggcccgaaca aatcaaacgg gagttcctgg cggagcttgt
      541 aaaaaagttt ggaaataaca atactgacca ccgccggagt ggttttttta attacattga
      601 tcacgggtga cttctcgagc ataaatttag atttaagatc aaacttacat taactaaaaa
      661 ttaaaacttc aatataatta taacatacta aaaaaatagc ttaaaggttt gaaatatttt
      721 ataatgaatt aatgctcaaa catcttaaac aaattttaat attagcaata atcatagtca
      781 gctttggtct ggccggttca gccctggctc aaaatgtcac tgttaataac ggcgacctga
      841 tcaaaggcgg tagtccggcc gtttattatt atttagacgg ccagcggttt gtttttccca
      901 atgaaaaaat ctttaaatcc tggtttgctg attttacaac cgttaaaatt atcccggatg
      961 aacaactggc ggccatccaa attggcggca atatcactta ccggcccggc gcccggctga
     1021 ttaaaattat ttccaacccc aaagtttacg ccgttgagcc gggcggcttg cttcgctggg
     1081 tcaaaaccga agaactggcc cggctgctgt ttggagacaa ctgggcctta ctggttgatg
     1141 atttacctga atcattcttt gtcaattaga aaatcagcct gccgctggaa acagcccttc
     1201 acccgatagg cagtctgatc agttatccgg ctgaccctga tcaaatttat ttaattgctt
     1261 ttgaaaacgg tcaaattgtt aaaagactga tttccagtga agctttcagc gcgaataatt
     1321 tccaaaatgt caggatatta acggctgatc cctccattat ttatcaggac ggcagtccgc
     1381 tcaaccaggc tgaatccaaa ctgaccgatg tctcccaaaa aaccactgct ttggcttttg
     1441 gcgaggcggc tgacatcgcg ccggctgatg atatcattgg caatgacctg ctaacagaaa
     1501 agaaagaact ggcgatcttg ctcggttcgc cggccgtttc ccgggttgtt cagaaaaaac
     1561 cccaggcgga aattggtatc ttttccttta aagccagtca ggcgaccggc tataccatta
     1621 cctatttaag attaaacggt tttattgacc agaacaaagg caaccctgat tttggcctgg
     1681 ggcgggacga tgaccgtgac gggtcaactt atctcagccg gattttaaac aatatcaacc
     1741 tttataatga tcaagatgaa ttaatcgccg ggccggtcaa catgtctctt gatggttttg
     1801 ctgtttttaa taatctgact ttagctgtcc cctctgacca gacaattaaa attatcgtca
     1861 aagctgatgt taacaccgaa ctgctggccc aggacactta tcgtttttca attgatattg
     1921 tcagtgatct ggatatcagc gttgttgatg acaataacat taaaattatc ccgtccggct
     1981 taagccccaa taacggttta aatccttaca gttttttgac aattcaacgc aatggccaac
     2041 taaccgttga aaacgggccc aataacccgc caactgacat tgtcctgtcc ggccagcctg
     2101 atgttttaat ttattctcta aaatttaccg ctaaatacga accattttta gtccaaaaat
     2161 taggttttga aagaatcagc caggaagcta ataacaccat caaagaaata tcttttacct
     2221 atccaactca aaccgggcaa ataacggttt ccagtccttt ttctttcaat caggttaaat
     2281 tcactaatct cggtttttat ctcccggcca acacggattt taccctggaa attaaagctg
     2341 aaacttatcc ttaccaggga tattataaca gcggttcgga aatcaaaatt aaaatgctgt
     2401 atgatgaaaa ttttattgtc aaaggacttg aatcgggagt tgagctgacc tattttgatg
     2461 acaccgttgg taatttattc aataaaacaa ccccgagcaa tactttaatc gtcagacaaa
     2521 caaaactgtc agccagacta agcccggaaa ctccggaaag tttatccagt cgcggtttta
     2581 atccggtctt gaggtttgaa gtaacggctg atccggctgg cccggcccgg ttgagaaaac
     2641 tgacctttaa agtcacttct tccagcgtaa attttattgg cgatgataat gatttatttg
     2701 aacgctgggc tgacgtcaac ggcgacctgc tggacgatga cgatgtcgcg gcgatttata
     2761 atctcaatga tttaaataaa agcctggctg gcggatttaa cggccgggtg gagtttgcca
     2821 tttttgatca ctccgccaat acccttgata ccactcccca gggctttcag actgaagcca
     2881 atgattatgg aataatcaaa atcagctttg ccagccagcc gctgatttca gccggccaga
     2941 ctgaaacttt tatcctgtat cttaattccg cctatattgg cgatcagggg gccactttaa
     3001 aagccgagct tttgggcgat aatcaaaatg ccaattttac caataacaat tttgagtggc
     3061 acgacggcca gatgccggcc accggttatt taataaaaaa tctaccgatc ggcggtaaac
     3121 tgttaacatt cgcggagcaa taaaaagcaa ggcaataggc acgaggcaca aggcaacagg
     3181 gattgggcat tagtaaaaac ccttttgact tatgcccatc aaataattgt cccgtcagtc
     3241 gcgactgacg ggacaccaac ccttgtgcct attgccttgt gccttgtacc tgccccgccg
     3301 cggcgggaca taaaaaaaag agcgatctct caatcgctcg gtgtcgcggc tacttttttt
     3361 cagagtgagc tgcctccagc ccgctggtta ccttttccca caatcccacc agctggtcaa
     3421 tccaggagcc caatgtcgcc acgtcctctt ccagcttttg aagacgcttt tggtggcctt
     3481 tcggcgactc ttttttcaac tgggccacct gctgttccag gccggtcaac cgttctttga
     3541 gcttattcag ctcgtcctgg ttggcggtag ctgtctcaaa ctgtggggtc tcggcctttt
     3601 ctgtcagttc gttgactttg gtggccaggc tggtaatctg ctgacggtcg ttgcccaaca
     3661 gaaaaccaaa cagcaaaacg gacagcagaa ccgaaacggc aacagcaatt gctgaactaa
     3721 cgacagccac cctaatcggc tgacctgtgg cggtggattg gccatcggcg tcctgctcca
     3781 gtttgttgac ccggttaaga agggtgtagg aatcgccagt gaagacctcc ttgccgagct
     3841 cggcgtcata atcttcgagc ttgtcaagcc tttttcctat actgacgata acgctgataa
     3901 tcatgtcgaa aaactttgca acccgggtgg aaagttgtct gttgcttttt tcctgtcctt
     3961 caactccttt ttcgagcatt aacaaatcgt tattgagttc tacgcacaaa ctctccaaat
     4021 cctggacctg tgtggccaag tcagcattgt ctgacattat ctcctccttg tcaaaggact
     4081 ctgtttacat acctccagac tcaataagct aacatcaaaa ggcgaggttt gtcaagcaag
     4141 gtgtatcagg gattatcagg ggatagcaag ggaaatcagg ggagataatg ggaatcaaag
     4201 ggcatcagag ggaagtaaat gtaaattaat agttccttaa aattagtata attagtatgt
     4261 catttgcata ttggcatcgc gtattggtat cccaaaaaaa atgagagact attcatctct
     4321 caacgagccc ccctacttct tgttctcctt tttcggctca ccgttttttg ccggctggag
     4381 cttggccagt tcttgctgaa gcctggcgac ttcccgtgag aggttagcca gactgtcagc
     4441 cagcttctgg gcgctggcaa cggcgtcttc tttggactga aaggccaggc ttttattcag
     4501 ccagtcggtc aaagtttgat ccgacggcac ccggcccaaa gtcttgttta tctgctgacc
     4561 atggccttcc accagctgca tcagattttc cagcttttga tcaagctgtt tggctgaatc
     4621 tgcctggttt tgctgatttt ctgtttgtcg gccttccaga atggccagct gatcagtcat
     4681 ctcctgaaac gactgctgat aaccggcaag ctgatcagtc acggccgcct cagtcaccag
     4741 ctcaggctgg ccggtcgcct gataataggc gtcagccagc tgctgatcgc tagcgtaagc
     4801 cagaatagca aataacaaac cgagaaaaag aaaactactc atggcaaaga ttcttgttcg
     4861 acgaagctgt ctgactaaag agccggcgat ttcgtcaact ttgtgactca gggaagcagt
     4921 ctcttccttg ttattcctgc cctcgtcggc tatctgtttg acccgatcca tgttttgtct
     4981 gacaatggac gcgttagtcg tgctctggct gacagattca ctgaatcttt gctgttcagt
     5041 tgccaaagcc tcaatttttg cccgggcaac ttcatcagct ccatttgcta caagcctctc
     5101 aattgctttt tcagaagcaa gcgacattct gtcgatagct gtttcaaaag ctttgaccaa
     5161 acgggtgagc tccgccgaac caccaaccgg cccgttagcc aaagcctggt gaataccagc
     5221 caccgttttg ccaatttctt cctgaacccc ttgtgattca gtcagccttg cctgtaaagc
     5281 cgcaagctct ttatggaagc tatccatttc attaatcagc tcgcagagct gttttctgta
     5341 tccgtctctt tccgattcgg gctcttgccc tttgcccatt gaaaaacctc ctccaaacag
     5401 ttccggggac accaccacct tatcaggttt atataccaga gtcaattagt tgacaactaa
     5461 gccaatttat ttaaaaataa acgactgttc tttgaaagga ggcaaagatg tgggctgatg
     5521 atgacgaaga cgatgactgg atggacgacg acctgaatgg agaaaccgaa gaaacttatt
     5581 ccggcttgtc agttttcagt tcagattact gggaagcggt tcaccaggaa agagagcgcg
     5641 acaaaaaaac tgacaaagat ttcaggcttt atgtctggct ggtgctcttt ataattatgc
     5701 ttttcggctg tctggtggtt tcagtgctgg ctaacctggg gctgatcaaa ccgcggctgg
     5761 tttatgtcat tttgcttggt ctgggtattg ggctggccct tcgcctgacc ggcatcttcc
     5821 gcgaacagta aaaaaccgat tgctttggag ggcagtcggt tttctattta gttaacgtta
     5881 tacgaatcta caaattttat ccgaatctac aaattacgaa tattttttct gcgaatatta
     5941 aatttattta gtggtgccgg cttcttcttc aatttcaatt tcaacgccga ca
//
LOCUS       DASWHZ010000090         6610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2785575, whole genome shotgun sequence.
ACCESSION   DASWHZ010000090 DASWHZ010000000
VERSION     DASWHZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785575"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="VGA49_02015"
     CDS             <1..2175
                     /locus_tag="VGA49_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664577.1"
                     /translation="NHLAIADKLIAAGQGGSVADNLDKFKGLNHLTIADKLIEARQGK
                     SVAKNLDKFKGLDHLAITDKLIDAGEGVSVANNLDKFKGLDYQVIADKLIEAGQGEYV
                     AWNLDKFSGLDSTIADKLIAAGEGVSVANHLDAFSGLDYQVIADKLIEAGQGEYVAWN
                     LDKFSGLDSTIADKLIAAGEGKYVAKNLDKFSGLDQAFGKRFLDQLKTDLETNFSLAL
                     AVNERFKLDKDLTKVGNIFGEFAGYDNYSLLKNLNSGELTADQRYQLETLGVKNEGQK
                     GEQELQEALIKFQESVISAKGPDIELIRENSFLRDYFKKLIRYEESEWGEHGPESFER
                     LLDRYDESALRELPKGYAESQVQRIDKIDRSAQESFEFTAGGLKRYQIFIEAIEAGLR
                     ANDNPKALTAVIEELENKQQELISQLEQKLEHLKQAKGRESLEMKIKALRQLDFKNQE
                     NWQEMFTVLASYGDTFKKELLTALFWWSFKFRPEEVGPAKEHLKNKNEPGLDDFSWAI
                     NFVEHITNKETLKQYFTDKRASAELKKILSVKAMSVELERAQNQPVKGTLTMKFIPQR
                     NILTEFSGQIGDACWADKYDSILKRYPNFISLMMVTNAEDPKTRRLAGSAFLIETKSA
                     TGDDLLVIRGLNPLENIINQLSQKDFINKLIGYLRPMAEKQGRKLAIVIDGHSGGSGT
                     NRPVLFNYLSRIKNELKKSELASDEDTKFNGYDIKKATYLIS"
     gene            2190..3653
                     /locus_tag="VGA49_02020"
     CDS             2190..3653
                     /locus_tag="VGA49_02020"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9664578.1"
                     /translation="MPRLELKKITNNIWQLPRHEDMLVPGLIYGNQEIIDLLLADLDK
                     PEKQWNSLLQIYNVACLPGIQKYMLAMSDVHPGYGFPIGGVAAFDLDSGIISVAGVGF
                     DINCGVRTLTTQMTRADLTDEKKEKLANQLYRDIPAGVGVDGHIKLKPEEMDQVLTEG
                     ARFSIQKGYGTEEDLKYIEENGRIANAKPKNVSDKAKKRGLKQIGTLGSGNHYLEVQS
                     VEKIFDKQAAEAFGLFQDQLLITIHCGSRGLGHQIGSDYLKTLELASKKYKIPIRERE
                     LVCAPFKSPEGQAYYTAVNCGINSAFANRQAIAHLTREAFSHALGIKPATIHTLYEVA
                     HNTVKVEQHLVDGKKKKLVVHRKGATRAFGPGHPEIPKKYQPVGQPVLIGGSMGTYSY
                     ILNGTEKAMTDTWGSACHGAGRSMSRAEAKRTWRGTELVKELAERGILIKSRSMSGVA
                     EEAPGSYKDVDVVVDVMHNAGIVNKVVRVRPVITVKG"
     gene            3828..5402
                     /locus_tag="VGA49_02025"
     CDS             3828..5402
                     /locus_tag="VGA49_02025"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9664579.1"
                     /translation="MKNKPRIYRFFELIPGILIWTTFILGVVLSFIRPIWVIYFMIVF
                     SLYWLFRVVYFVIYICISWYRYRRDLTADWLAQVKDKPDWQRIYHLVFLPTYKEPLEV
                     LRSTFQSLINSDYPLDKFIVVLAGEERDLENFKNNAEVIKKEFNDKFFKLLITYHPDN
                     LPDEIKAKGANAHWAGQRAQELIDRLGIPYQDIIVSYFDIDTVVHPKYFSCLTYKYLT
                     DPEPLRSSYQPVVLYNNNIWDAPSMMRITAFGTTFWLMTELPKPDRIFTFSSHSMSFQ
                     ALVDVNFWQKDIVTDDSRIFLQCFVRYDGDYKIVPLYVPVSMDTVNADGYWRSFVNLY
                     KQQRRWAWGIEHFPYMIWNFFFKKNKISLKKKVKCLWNFSEGMYSWATAPILLFVFGK
                     LPLWLAGPQAQATVIVQSAPFVLQWVLSLAMIGIFVSATFSLMLLPPRPRQHAPHKFL
                     IMILQWILLPISIVVFGAIPAIESQTRLMLGKYLGFWVTEKSRKSADQPLVSQPVIIT
                     AGSSRPDNKEHLISEI"
     gene            5435..6466
                     /locus_tag="VGA49_02030"
     CDS             5435..6466
                     /locus_tag="VGA49_02030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9664580.1"
                     /translation="MPKVSVNLVTWNGLKYLADCLESIYQQTFQDFSLIVIDNGSTDG
                     TVEFIKTKYPQAAVIQNFKNLGFARAHNQAIEYSNTDYVLLINQDIILNSDYLEQLIK
                     TVETDSKIGSLGGKLLKIKTSQDDLNQKIKTSLIDSAGLTIFKNRRVIDRGSGQEDQG
                     QYNQPEEIFGFSGALVLYRRQALNDIKLDHPGKKISEYLDQDFFSYKEDIDLAWRLQL
                     AGWKSVYLPSAVAYHYRTASQAENPGWQAIRINRRDKSNFVNQLSYKNHLLLLFKNEA
                     LANFLKFSPQIVSYELKKFFYLLIFEPANWYRSLKKFFKQLPRILSKRKIYQEKIRLN
                     SGQLNRWFI"
BASE COUNT         2010 a         1211 c         1544 g         1845 t
ORIGIN      
        1 aatcatttgg caatcgccga taaattgata gccgcggggc aaggcggatc ggtggctgat
       61 aatttagaca agtttaaggg attaaatcat ttgacaatcg cagacaaatt gatagaagct
      121 agacaaggca aatcagtggc taaaaattta gacaagttta agggattaga tcatttggca
      181 atcaccgata aattgataga tgcgggggaa ggtgtatccg tggctaataa tttagacaag
      241 tttaagggat tagattatca agtaatcgct gacaaactga tagaagcggg gcaaggtgaa
      301 tatgtggctt ggaatttaga caagtttagc ggattagatt caacaatagc cgataaattg
      361 atagccgcgg gggaaggcgt atccgtggcc aatcatttag acgcgtttag cggattagat
      421 tatcaagtaa tcgctgacaa actgatagaa gcggggcaag gtgaatatgt ggcctggaat
      481 ttagacaagt ttagcggatt agattcaaca atcgccgata aattgatagc cgcgggggaa
      541 gggaagtatg tggctaaaaa tttagacaag tttagcggat tagatcaagc ttttggcaag
      601 agatttttag atcaactgaa aacagattta gaaacaaatt ttagtttagc cctggctgtt
      661 aatgaaagat ttaaacttga taaagattta accaaagtcg gcaatatttt tggtgaattt
      721 gccggttatg ataattatag tttattaaag aatttaaatt caggagaatt aaccgctgat
      781 cagcgctatc aattagagac attgggagta aagaatgagg ggcaaaaggg agaacaggag
      841 ttacaggaag ctttaattaa atttcaggaa tcagtcatca gcgccaaagg cccggatatt
      901 gaattaatta gagaaaactc ttttttaagg gattatttta aaaaattgat tcgatacgaa
      961 gaatctgaat ggggcgaaca cggcccggaa agttttgagc ggcttttaga cagatatgat
     1021 gagagcgctc taagggaatt acccaagggc tacgccgaga gtcaggtaca gcgaatagac
     1081 aagatagacc ggagcgccca ggaatcgttt gagttcacgg ccggcggtct caaaagatat
     1141 caaatattta ttgaagcgat tgaggcaggt cttagggcta atgacaaccc aaaagcgctg
     1201 acagccgtta ttgaagagct ggagaataaa cagcaggaat taatcagcca gctggagcaa
     1261 aagctggagc atttaaagca ggccaaaggc cgggaatcat tagagatgaa aattaaagca
     1321 ttaagacaac tggattttaa aaaccaggaa aattggcagg aaatgttcac ggtcctggct
     1381 tcttacggcg atacatttaa aaaagaactg ttaacggcgt tattttggtg gagttttaag
     1441 tttaggccgg aagaagtcgg gccagccaag gaacatctta aaaataaaaa tgaaccggga
     1501 ttagatgatt tttcctgggc aattaatttt gttgaacata taaccaacaa agagacttta
     1561 aaacaatatt ttacggataa acgggccagc gctgaattaa agaagatatt aagcgtcaaa
     1621 gctatgtcgg ttgaactgga acgggcgcaa aatcagccgg tcaagggcac tttaacgatg
     1681 aaatttattc cccagagaaa tattttaact gaattttcag ggcagattgg cgatgcttgt
     1741 tgggcggata aatatgacag tattctgaag agatatccaa attttatatc gttaatgatg
     1801 gttaccaacg ccgaagatcc caaaacccgg cggttagccg gcagtgcctt tttaattgaa
     1861 actaaatcag caaccggaga tgatctttta gtcatcagag gattaaaccc gctggagaat
     1921 ataatcaatc aattgtcaca aaaagatttt atcaataagt tgatcggtta tttaaggccg
     1981 atggctgaaa aacaaggcag gaaattagca attgtcattg atggtcattc tggcggttcc
     2041 ggcactaatc gcccggtact gtttaattat ttatccagaa taaaaaatga attaaaaaaa
     2101 tcagaactgg cctctgatga agatactaaa tttaatggat atgatataaa aaaggcaaca
     2161 tatttgatat cataaattgt ttaatataaa tgcccagact cgagctcaaa aaaattacca
     2221 acaatatctg gcaactgccc cggcacgaag atatgctggt tcccgggctg atttacggca
     2281 atcaggaaat tattgattta ttgctggctg acctggacaa gccggaaaaa caatggaatt
     2341 ctttacttca aatctataat gtcgcctgcc tgcccggcat tcaaaaatat atgctggcca
     2401 tgagcgatgt tcatcctggc tatggctttc caatcggcgg ggtggcggct tttgatctgg
     2461 acagcgggat tatttcagtc gccggggttg ggtttgacat taactgcggg gtccggactt
     2521 taacgaccca aatgaccagg gctgacctga ctgatgagaa aaaagaaaaa ctggctaacc
     2581 agctttatcg ggatattccg gcgggcgttg gagttgacgg gcatatcaaa ctgaagcctg
     2641 aagaaatgga tcaggtttta actgaaggcg cccggttttc aattcaaaaa ggctatggca
     2701 ctgaagaaga tttgaaatat attgaggaaa acggacgaat tgccaacgcc aaacctaaaa
     2761 atgtctctga taaagccaag aaaaggggac tgaaacaaat tggcaccctg ggatcaggca
     2821 atcattatct ggaagttcag tctgttgaaa aaatatttga taaacaggcg gctgaagctt
     2881 ttggtttatt tcaggatcag cttttaatta cgattcactg cggttcgcgc gggctgggcc
     2941 atcagattgg atcagattat ttaaaaacgc tggagttggc ttccaaaaaa tataaaattc
     3001 caatcaggga aagggaactg gtctgcgcgc cgtttaaaag tccggaaggc caggcttatt
     3061 acacggccgt caactgcggg attaattcag cttttgccaa ccgccaggcg atcgctcatc
     3121 tgacccggga agctttcagc catgctctgg gcattaagcc cgcgacaatt cacactcttt
     3181 atgaggtagc ccataacacg gttaaagttg agcaacattt agttgacgga aaaaagaaaa
     3241 agctggttgt tcaccgcaaa ggcgctacca gggcttttgg gccggggcat cccgaaatcc
     3301 ccaaaaaata tcagccagtc ggtcagccgg ttttaattgg tggttcaatg ggaacttact
     3361 cttacatttt aaacggcact gaaaaagcca tgactgatac ctggggatca gcctgccatg
     3421 gcgccggtcg gtcaatgtcc cgggctgaag ccaagagaac ctggcggggg acggaacttg
     3481 ttaaagaatt agctgaacgg ggtattttaa tcaaaagccg gagcatgtcc ggcgtggctg
     3541 aagaagcgcc gggatcatac aaagatgttg atgtcgtggt tgatgtgatg cataatgccg
     3601 ggatcgtcaa taaggtggtc agggtcaggc cggtgattac ggtgaagggg taaaatcggg
     3661 caacaggcaa taggcacaag gcacaagggt tggaatattc attagatgag gatcaggtaa
     3721 agggataaat agagtgggga gattttttac aattctggtt ttggccggga tttgattttt
     3781 tttaccaaaa attgtatgat ttatacacta attttacttt tttaagaatg aaaaataaac
     3841 ctcgaattta ccgatttttt gaattaatac caggaatttt aatttggacc acctttattt
     3901 tgggagttgt cctttccttt atcaggccaa tctgggtgat ttatttcatg attgtcttca
     3961 gtttgtactg gctttttcgg gtggtttatt ttgttatcta catttgtatc tcctggtatc
     4021 gctaccgacg tgatttaact gctgattggc tggctcaagt caaagataaa ccggactggc
     4081 agagaatcta tcatttggtt tttctgccaa cttataaaga accgctggaa gttttacgat
     4141 caactttcca aagcctgatt aacagcgatt atcctttgga taaattcatt gtcgtcctgg
     4201 ccggggaaga acgagatctg gaaaatttca aaaataatgc ggaagttatt aaaaaagaat
     4261 ttaacgataa attttttaaa ctgttgatta catatcatcc ggataatctg cctgatgaaa
     4321 tcaaggccaa aggagccaac gctcactggg ccggccagcg ggcccaggag ctgattgacc
     4381 ggttggggat tccttatcag gatattattg ttagttattt tgatattgac acggttgttc
     4441 atcctaaata tttttcctgt ctgacttata aatatttaac cgatcccgag ccattaagaa
     4501 gttcttacca gccggtagtt ttatataaca ataatatttg ggacgcccct tcaatgatga
     4561 gaattacggc ttttgggacg actttctggc tgatgactga actgcccaag cctgaccgga
     4621 tttttacttt ttcatcccac tcaatgagtt ttcaggccct ggttgatgtt aatttctggc
     4681 aaaaagatat tgtgactgac gactcccgga tatttttaca atgctttgtc cgctatgacg
     4741 gcgattataa aattgtgccg ctttatgtgc cggtctcaat ggatacagtt aatgctgacg
     4801 gttactggcg gtcatttgtt aatttatata aacagcaaag gcgctgggcc tggggaattg
     4861 agcatttccc ttatatgatc tggaatttct tttttaagaa aaataaaatt agtttaaaga
     4921 aaaaagttaa atgtctctgg aattttagcg aaggcatgta ttcctgggcg accgccccga
     4981 ttttattgtt tgtttttggc aaactgccgc tctggctggc cggtccgcag gcccaggcga
     5041 cagttattgt ccagtcagct ccgtttgttt tacagtgggt tttgtcgctg gcgatgattg
     5101 gtatttttgt ttcagctact ttcagtttaa tgctcctgcc gccccggccc cgccagcacg
     5161 ctcctcataa atttttgatt atgattttgc agtggatttt attgccgatt tcaatcgttg
     5221 tttttggggc aattccggca attgagtcac agaccagact gatgcttggc aaatatcttg
     5281 gtttttgggt aacggagaag agccgtaaat cagctgatca gcctctggtc agccagccgg
     5341 ttattattac agcgggcagt tcccggcctg ataacaaaga acatttaata tctgaaatat
     5401 agaataagcc actagtgact aattactact acttatgcct aaagtctctg ttaatctggt
     5461 cacctggaac ggcctgaaat acctggctga ctgtctggag tcaatttatc agcaaacctt
     5521 tcaggatttt agtttgattg taattgataa cggctcaacg gacggcacgg tcgaatttat
     5581 aaaaaccaaa tatcctcaag ccgcggtcat tcaaaatttc aaaaatctgg gctttgcccg
     5641 ggcccataac caggcgattg aatattccaa cactgattac gttttattga tcaatcagga
     5701 cattatcctt aatagtgatt atttggaaca gctgatcaaa acagttgaaa ctgattctaa
     5761 aattggatcg ctgggcggga aattattaaa aattaaaacc agtcaggatg atttaaatca
     5821 aaaaattaag accagtctga ttgattcagc cggcctgaca atttttaaaa accgccgggt
     5881 gattgaccgg ggcagtggtc aggaagatca gggtcaatat aatcagccag aagagatttt
     5941 cggcttttcc ggagcgttgg ttttataccg gcgccaggct ttaaatgaca ttaagctgga
     6001 tcatcccggc aaaaaaatca gcgaatattt agaccaggat tttttttctt ataaagaaga
     6061 cattgatctg gcctggcggc tccagcttgc cggctggaaa tcagtttatc tgccgtcggc
     6121 cgtggcctat cattaccgga cggcttctca ggccgaaaac cccggctggc aggcaatcag
     6181 gatcaaccgg cgggataaat caaattttgt taaccagttg tcatataaaa atcatttgct
     6241 gctgttattt aaaaacgaag ctttggcaaa ttttttaaaa ttttcgcccc agattgtgtc
     6301 ctatgaacta aaaaagtttt tttatctttt aatttttgaa ccggccaact ggtaccggag
     6361 cttaaagaag ttttttaaac agctgcccag gattttatct aaaagaaaaa tttatcagga
     6421 gaaaatcagg ctgaattccg ggcaactaaa caggtggttt atttaaagca atggatttat
     6481 caattattat tctcaactat aaaaccaggg gactgcttaa aaattgtctg aatggcctca
     6541 agcggtaccc gcctgactgc cattacgaga ttatagtcgt tgataatagt tcaaaggacg
     6601 gaacagccag
//
LOCUS       DASWHZ010000091         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1297844, whole genome shotgun sequence.
ACCESSION   DASWHZ010000091 DASWHZ010000000
VERSION     DASWHZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297844"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..503
                     /locus_tag="VGA49_02035"
     CDS             213..503
                     /locus_tag="VGA49_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9664581.1"
                     /translation="MANLKPLSDHVIVKPLEEEKTKSGIILPDTVDKERPEKGEVVAV
                     GPGKILENGSRAPMSVQVGQKVVFKKYSPDEVKVDDQELLVISESDIIAVIE"
     gene            538..>2073
                     /gene="groL"
                     /locus_tag="VGA49_02040"
     CDS             538..>2073
                     /gene="groL"
                     /locus_tag="VGA49_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985894.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9664582.1"
                     /translation="MAKQILYNEEARTALKRGVDKLANAVKVTLGPKGRNVVLDKGYG
                     APTITKDGVTVAKEIELEDKFENIGAELVKEVASKTNDVSGDGTTTATILAQSMITEG
                     LKNVTAGINPLMIKRGIDKGVEALVAELRNISKPVSNDEEIAQVASISANDPAIGKII
                     AEAMKEVGQDGVITVEESQSFGIEKEKVEGLQFDKGYLSPYMITSAERMTAEYDDPQI
                     LITDKKISSINEILPLLEKLAQSGKKELVIIAEDVDGEALATLVVNKLRGAFNALAIK
                     APGFGDRRQAMLEDIAVLTGGRLISDEVGLKLENVELTDLGQARKIKSTKEDTTIIEG
                     RGDQAAIADRVKQIKLELDRADSDFDKEKFQERLAKLSGGVAILKVGAATETEMKEKK
                     HRVEDALEATKAAVEEGVVPGGGVALIRAVRALDNLEVSAEEKVGLNILRKAIEEPLK
                     QIAFNAGVEGTVIVEEVKKREANFGYNAATNQFEDLVQAGIIDPTKVTRSALQNSASI
                     AAMM"
BASE COUNT          591 a          437 c          546 g          499 t
ORIGIN      
        1 cttctgattc cctctgattc cctctgattc cctctgattc cctctgattc cctctgtttc
       61 cctctgattc cctctgcttc cttttgattc ccttcccccg tcttgacaga ttcttgccaa
      121 tttgttaacc tattttaata aaatttagca atctttatgg tggactgcta aaatttttta
      181 tccaggaaaa taattaattt tttgtaaaaa atatggctaa tttaaaaccg ttaagcgatc
      241 atgttatcgt caagccgctg gaagaagaaa aaaccaagtc tggcattatc ctgcctgata
      301 cggttgataa agaacggccg gaaaagggcg aagtggtggc agtgggtccg ggcaagattc
      361 tggagaacgg ttcacgggcg ccgatgtcag ttcaggtcgg ccaaaaagtt gtctttaaaa
      421 aatacagccc ggatgaggtc aaagttgacg accaggaact gcttgttatt tcagagagtg
      481 atattatcgc ggtgattgaa taaacgttat ctaataaaaa ttattaaacc acgaactatg
      541 gctaaacaaa tcttatacaa tgaagaagcc cggaccgctc tcaagcgggg ggttgataaa
      601 ctggccaacg ccgtcaaagt tactttgggt cccaaaggcc ggaatgttgt tttggacaaa
      661 ggctatggcg cgccaacgat taccaaagac ggcgtgacag tcgccaagga aattgaactg
      721 gaagataaat ttgaaaatat cggcgccgag ctggtcaaag aagtcgccag caaaaccaat
      781 gatgtttcag gcgacgggac gacgacagcc actattctag cccaaagcat gattactgaa
      841 ggtctgaaaa atgttaccgc cgggattaat cccttaatga ttaaacgggg gattgacaaa
      901 ggcgtggaag ctttggtggc tgaacttcga aatatttcca aaccggtcag caacgacgag
      961 gaaatcgccc aggtggcttc tatttccgcc aacgatccgg ccatcggcaa aattatcgct
     1021 gaagcgatga aagaagtcgg ccaggacggg gtgattacgg tggaagagtc ccagtctttt
     1081 gggattgaaa aagaaaaagt tgaagggctt cagtttgata aaggctatct gtcgccttac
     1141 atgattacca gcgccgaacg gatgacggct gaatacgacg atccccagat tttaattact
     1201 gataaaaaga tttcttcaat caatgaaatt ttgcctttgc tggaaaagct ggctcaaagc
     1261 ggtaaaaagg aactggtgat tatcgctgaa gatgttgacg gtgaggcact ggcgactttg
     1321 gtcgttaata aactgcgggg cgcctttaac gctctggcca tcaaagcccc gggttttggc
     1381 gaccgtcgcc aggccatgct ggaagacatc gcggttttaa ccggcggccg gctgatttcc
     1441 gacgaagtcg ggctaaaact ggaaaacgtt gaactgactg atttgggcca ggccagaaag
     1501 attaaatcaa ccaaagaaga cacgacgatt attgaaggcc ggggtgacca ggcggcgatt
     1561 gccgaccggg ttaagcagat taaactggaa ctggaccggg ctgattctga ttttgacaaa
     1621 gaaaaatttc aggaacggct ggctaagctg tccggcgggg tggctatttt gaaagtgggc
     1681 gcggcgactg aaactgaaat gaaagagaaa aagcaccggg tggaagacgc tctggaagcg
     1741 accaaagcgg ccgtggaaga aggcgtcgtg ccgggcgggg gcgtggcttt gatcagagct
     1801 gtccgggccc ttgataattt ggaagtttca gccgaagaaa aagtcggttt gaatattctt
     1861 cgcaaagcga ttgaagagcc gctgaagcag attgctttta acgccggcgt ggaaggaacg
     1921 gtcattgttg aagaagttaa aaaaagggaa gccaactttg gctacaatgc cgccaccaat
     1981 cagtttgaag atttggttca ggccgggatt attgatccga ccaaggttac acggtcagct
     2041 ttgcaaaatt cggcttcaat cgccgccatg atg
//
LOCUS       DASWHZ010000092         7814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_575806, whole genome shotgun sequence.
ACCESSION   DASWHZ010000092 DASWHZ010000000
VERSION     DASWHZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575806"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..418
                     /locus_tag="VGA49_02045"
     CDS             35..418
                     /locus_tag="VGA49_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664583.1"
                     /translation="MTKKAKKTIYWSIGGAVVIGFTAWLIVGLSQPGPLDAFAKCLKD
                     KGATYYGAFWCPNCQRQNDLFGKSKKHTPYVECSTPDGQGQKQVCQQAEITNYPTWEF
                     ADGSRDIGVQPLEDLAEKTGCELPR"
     gene            421..990
                     /locus_tag="VGA49_02050"
     CDS             421..990
                     /locus_tag="VGA49_02050"
                     /inference="COORDINATES: protein motif:HMM:NF002849.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX9664584.1"
                     /translation="MSQLTSTVTTILSVLTIIGQVFIGLAIIWLLIYRFKKEQPVVTK
                     VLNFFDKRAVLFSLIVALLATLGSLFYSEIAGFEPCRLCWYQRILMYPQVILLALALW
                     IRDKKNIVVYSLGLSIVGAVIAGYHYLLQIGVVKSSSCEVVGYSVSCSQRFVMNFGYI
                     TIPMMALTAFGLIIVFMIIKKLASSRSYE"
     gene            983..2572
                     /locus_tag="VGA49_02055"
     CDS             983..2572
                     /locus_tag="VGA49_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664585.1"
                     /translation="MSKKSSIIIGILIVIVILAIGWFFLKDKIFPGVITPEAGGTVVS
                     SDGRLKLAIPAGAVSEKVRIKVSPAVPGPGLEFAEDVYELSPSGLEFLKPVIISLTLD
                     QTDSEEGQLAGEIQLLYSLQADGTIEPLAEQALEFEEGGQAVVNGQLSHFSEVLRAPT
                     GVSVTLTGVPDVIQVDKKFLVNVTVVKKVDSEVFNIDQVTYRSNGEDPIIFWEDIRGF
                     APSRRQSDLLIGRRLTEAAGEQIITKNDLRHSCVAPAYPNFNSSILFQDIQVTLGLLG
                     DLEFPPVSKYEIKGKKKILCKGDLDKVTDRFQQTNIEFELKVDPANVSKLINQSFNIT
                     ISEKPQQYIGQVRLRLADTNPGIVSAPRLADDQSEGGTFIFKLDPVKGKEDATQQLEL
                     IGLKTTEENKQKLVYTCLKPGETDLVYFINEFSLDESWSFQSAPRAVSSHVRCLSEEE
                     KVEDAASIQAIKYLDKFIPLDEVHQASPDACGEVHWHANKELATATDGAKIPDPNPFA
                     CGFGRVSEVPVVEVEIGRIGN"
     gene            complement(2574..3761)
                     /locus_tag="VGA49_02060"
     CDS             complement(2574..3761)
                     /locus_tag="VGA49_02060"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9664586.1"
                     /translation="MSDNEINFIDQQEIKNKRVLVRVDFNVTMNPDYTIADDARITQS
                     LPTINYLLENNNKLIIISHLDRPRGRDPKYSLKNIVANIEKYLPNYKITLVDDFLTAD
                     QSFFSNQNQKEIILLENIRFYPEEKNNNIEFSKKLSALADLFVNDAFGVSHRTDASVI
                     GVPSFIPSLGGLLLKKEIEMISTVFAPRQKPLVAIIGGAKISTKINFINKLMDKADAL
                     LIGGGLANTFLMAQGFNIGESIHEYEKTESARKLLYLAAQKNTAIVLPLDVIVGNPNN
                     GQNGGQVKRIEEVNSADHILDIGPETEARFGTIISKARTIVWNGPVGYIENPSFRRGT
                     DFIYYAITQNREATSIVGGGDTIAAISKKEYLDQISHISTGGGAMLEFIEQGTLPGIE
                     ALK"
     gene            complement(3773..4780)
                     /gene="gap"
                     /locus_tag="VGA49_02065"
     CDS             complement(3773..4780)
                     /gene="gap"
                     /locus_tag="VGA49_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9664587.1"
                     /translation="MKKLRIGLNGFGRIGRIFTRQALTKNSFDIVQINTRKKQPAMLA
                     YLLQYDSVYGPFQSAVIPGHDQIIAGNQTIKTTAFDDLNKTDWDNIDILVDATGVFLR
                     REDFSAPLANGVKKIIITAPAKDDTIDHCVLGVNDDQINFKKTEIIANCSCTTTAVAP
                     IFKLINDNFKIISGYLTATHSYTTTQNLLDNSHSDFTRSRAAGINIIPTTTGAAKAVE
                     KVLPELKGRLSGLALRVPLPTVSVADLSIVVEKQTNIEQVNQIIREAAQNSLAGILQF
                     ETQPLVSSDYIGSPYSGVVDGNYTEVMNGNLIKIFCWYDNEWGYCSRLIDLVEKFNQI
                     L"
     gene            4895..7072
                     /locus_tag="VGA49_02070"
     CDS             4895..7072
                     /locus_tag="VGA49_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9664588.1"
                     /translation="MSNLVVKSLVYIALTCGFLFFIYAVRYYLIIISVLLKHRGKRRP
                     SALQKISPINLKSCCGLLYGDNIAFRCNFNRHNHRKGRHGLLQRVDYLSLPKAPFISV
                     HIPFYNESKKVIDRVLGASLNLDYDHYEIIVCDDSTDGSLNYIKKKWGRKKGLKILHR
                     DNRIGYKGGALKNALRRMDSRTDFVVVFDADFIPYPDTLQLFLKYFIIANNGYFDFKK
                     SKIGAIQGYQWHVLNKDENWITKGIYTEFGGSYVFDRYGAEIFSGFKQIAGSVFMVRA
                     DLLNKIGWDDSITEDWELTLNLYQRGYKVIYTPHIQTPAECVNTLSKLRKQRTRWAEG
                     HTRDVRKHFFNILFSGNVNWREKLEFLFLSLFYLQSVFFVVGTISWLAVEFLFPYTKL
                     PWWTAMLGWSLVFTNFLALPLVNLAALFLQRSDKKDVLGILSFIVLGWYLAPFQTWAA
                     LKGLLIDRPSYWHRTPKSGRLSRLFRRKKQPEYFPAGEYIRAFFSKSKKFKFQPGHWL
                     VLGLLYFLFVFTLVFVLYSGTIKLARANPDEFYFHRTAAGGVQPSGKNMNSVPPAIQT
                     AFGVGDGAYYFYSGQYPAGATDTVIAPGNYIQTIWIEAGSSFSFNLDGEVSLCDPNGG
                     NCQTITSGSCSVAVSAGTNQSSCDLGAGQEQTIASANPQIIKIALIFNGLSDGNFLYD
                     GVNQSSAMSSPTVTVPEGVIWFSPVIIGLLFLIKPVLRRKQYV"
     gene            7065..7679
                     /locus_tag="VGA49_02075"
     CDS             7065..7679
                     /locus_tag="VGA49_02075"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEX9664589.1"
                     /translation="MSNFQTLEKQKYNLGTILLVATALILLLLPFVATFNEFLTSLAE
                     KVYLFRWIENVIVPFESRWVVGLLSFLGIESIATNSSVFLTEPAGVGLNVFISWNCIG
                     WQSFILLIFTFLTMLQGVGSGFQKMNVVLFGIIGTFWVNLIRIAAVILLAKFWGLKSA
                     ILFHDYAGNLMVIVWLLVFWLISFKYLLISRAILDKAQKFVYNA"
     gene            7686..7760
                     /locus_tag="VGA49_02080"
     tRNA            7686..7760
                     /locus_tag="VGA49_02080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7719..7721,aa:Gly,seq:tcc)
BASE COUNT         2043 a         1547 c         1848 g         2376 t
ORIGIN      
        1 ataaaaatta taaaataagc tataataaac cactatgaca aaaaaagcta aaaaaactat
       61 ttattggagt attggcggag cggtggttat tggttttacc gcctggctga ttgttggttt
      121 gagccagcca ggacccctgg acgctttcgc caaatgtttg aaagacaaag gcgccactta
      181 ttacggagct ttttggtgcc ccaactgcca gcgccaaaat gatctgtttg gtaaatccaa
      241 aaaacacact ccttatgttg aatgctccac tcccgacggt caggggcaaa aacaagtttg
      301 ccagcaggcc gagattacca actaccccac ctgggagttt gccgacggca gccgggatat
      361 tggcgtccag ccgctggaag atttagccga aaaaaccggc tgtgaactac cgcgataatt
      421 atgtctcaat taacttcaac agttaccacg atcttgtctg tcctgactat tattggccag
      481 gtctttattg gcctggcaat tatttggctg ctaatttatc gttttaaaaa agaacagccc
      541 gttgtcacta aggttttaaa tttttttgac aagcgggcag ttttgttttc tttaattgtt
      601 gccttgctgg ctacgctggg cagtttattc tattctgaaa tcgccggttt tgagccctgc
      661 cgactctgct ggtatcagag aattttaatg tacccccagg ttattctgct ggcgctggct
      721 ttatggattc gggataagaa aaatatcgtt gtttatagtt tagggctgtc aattgtcggc
      781 gcggtgattg ccggctatca ttatttattg cagattggag tggtcaaaag ttcttcctgc
      841 gaagtggttg gctattcagt cagctgttcc cagagatttg tgatgaattt tggttacatt
      901 acaatcccaa tgatggccct gaccgctttt ggcctgatta ttgtttttat gattattaaa
      961 aaattagctt cctccagatc ttatgagtaa aaaatcgtct ataattatcg gaattttaat
     1021 cgtcatcgtg attctggcga tcggctggtt ttttttgaaa gataaaattt ttccgggagt
     1081 gattacgcct gaagccggcg ggacggtggt tagttcagac ggcagactga agctggctat
     1141 tccggccgga gcagttagtg aaaaggtcag aatcaaagtc agtccggccg ttcccggccc
     1201 cggtttggaa tttgccgaag atgtttatga actaagtccc tccggtttgg aatttttaaa
     1261 gccggtcata atcagtttaa ctcttgatca gactgattcc gaagaaggcc agttggccgg
     1321 tgaaatccag ctgctgtaca gccttcaggc ggacggaaca atcgagccgc tggcggagca
     1381 ggccttggaa tttgaagaag gcggccaggc ggtggttaac ggccagttaa gtcatttttc
     1441 agaagtttta agggcgccga ccggggtgag cgttacttta accggggtcc cggatgttat
     1501 tcaggttgat aagaaatttt tagtaaacgt gactgtcgtt aaaaaagttg attcagaagt
     1561 ttttaatatt gatcaggtta cctatagaag taatggtgaa gatccaataa ttttttggga
     1621 agatatcagg gggttcgctc cctcacgccg acagagcgat cttttaattg gccggaggct
     1681 aacggaagcg gccggagaac aaattattac taaaaatgat ttaagacaca gctgtgtcgc
     1741 gccggcttat ccaaatttta attcttcaat tttatttcag gatatacaag ttaccttagg
     1801 tttattggga gatttggaat ttccgccggt tagcaaatat gaaatcaaag gtaaaaagaa
     1861 aattttgtgc aaaggcgatc tggacaaggt gactgacagg tttcagcaga ctaacattga
     1921 gtttgaattg aaggttgatc cagcgaatgt tagcaaactg attaatcagt cttttaatat
     1981 tacaatttca gaaaaacccc agcaatatat cggccaagtc agactgcgtc tggcggatac
     2041 caatcccggg attgtctccg ccccccggct ggctgatgat cagtcagaag gaggcacttt
     2101 tatatttaag cttgatccgg ttaaaggcaa ggaagatgca actcaacaat tggaattaat
     2161 cggactgaag actacagaag aaaacaaaca aaaattagtc tacacctgtc tcaaaccggg
     2221 cgagacagac ctggtttatt ttattaacga atttagtctt gatgaaagct ggagttttca
     2281 gtccgctccg cgagccgtta gtagtcatgt cagatgtctg tcagaagaag agaaggtaga
     2341 agatgccgcg tccattcagg ctatcaaata tttggataag tttattcctt tggacgaagt
     2401 ccatcaggct agtcccgatg cctgcggcga ggttcactgg cacgccaata aagaactggc
     2461 cacggccact gacggcgcga aaattcctga ccccaatcct tttgcctgcg ggtttggccg
     2521 ggtgagcgaa gtgccggtgg tggaagtcga aattggccga attggcaatt aaattacttc
     2581 aaagcctcaa tgcccggcag ggtgccctgt tcaatgaatt ccagcatggc gccgccgccg
     2641 gttgaaatat ggctgatttg atccagatat tcttttttgg aaatagcggc aatagtgtcg
     2701 ccgccgccaa caattgaagt tgcttcgcgg ttttgggtaa tcgcgtaata aataaagtcc
     2761 gtcccccgcc tgaaggacgg attttcaata tagccgaccg ggccgttcca gacaattgtt
     2821 ctggctttgc tgataatggt gccaaaccgg gcttcagttt caggtccaat atccaggata
     2881 tggtcagctg aattgacttc ttcaattctt ttgacctgcc cgccgttctg gccgttattg
     2941 ggattaccga caatcacgtc cagcggcagg acaatggccg tatttttttg agcggccagg
     3001 taaagcagtt tgcgggcgct ttctgttttt tcatattcat gaatgctttc gccgatgtta
     3061 aagccctggg ccattaaaaa agtattggcc aggccgccgc cgatcagcaa agcatcggct
     3121 ttgtccatca gcttattaat aaaatttatt ttggttgata ttttagcgcc gccaataatc
     3181 gccaccagcg gtttttgacg gggggcaaaa acagttgaga tcatttcgat ctcttttttt
     3241 aataataaac cgccgagcga ggggataaaa gacggaacac cgatgactga agcgtcagtc
     3301 cggtgggaaa cgccaaaagc gtcattaaca aaaagatccg ccagggcgga cagttttttg
     3361 gaaaattcaa tattattatt tttttcttca ggataaaaac ggatattttc cagcaaaatg
     3421 atttcttttt ggttttgatt ggagaaaaat gactgatcag ccgttaaaaa atcatcaacc
     3481 agggtgattt tataatttgg cagatatttt tcaatattgg ccacaatgtt tttcagagaa
     3541 tattttggat cgcgtccccg gggccggtcc aggtgggaga taatgattaa tttgttatta
     3601 ttttccagca aataattaat tgttggcagg gactgggtga tccgggcgtc gtcagcaata
     3661 gtgtagtccg ggttcatggt gacattaaaa tcaaccctga ccaaaacccg tttatttttt
     3721 atttcctgct gatcaataaa attaatttca ttgtctgaca tagtccgcaa ttttacaata
     3781 tttgattaaa tttttcaacc agatcaatca gccggctgca atagccccac tcattgtcat
     3841 accagcaaaa gattttgata agattcccgt tcatgacctc agtgtagttg ccgtcaacaa
     3901 caccggagta gggtgagcca atatagtcag aggaaactaa tggttgtgtt tcaaactgga
     3961 gaatgccggc tagcgagttt tgggccgctt ctcttataat ttgattgacc tgctcaatgt
     4021 ttgtctgctt ttcaacgaca attgacagat cagccaccga gacggttggc agcggcactc
     4081 ttaaggccag gccgcttaac ctgcctttga gttcgggcag gactttttca accgctttgg
     4141 cggcgccggt ggtcgttggg atgatattta ttccggccgc tctggaccgg gtaaaatcac
     4201 tatgagaatt gtccagcaga ttctgggtgg tcgtgtaaga gtgggttgct gtcagataac
     4261 cagaaataat tttaaaattg tcattgatta atttaaaaat tggggcgaca gcggtggtgg
     4321 tgcaggaaca gttggcaata atttcggttt ttttaaaatt aatttgatca tcattaactc
     4381 ccagaacgca gtggtcaatt gtatcatctt tggccggagc cgtaataata atttttttaa
     4441 cgccattggc cagaggagcg ctgaaatcct cccgtcttaa aaagacgccg gtggcgtcta
     4501 ccaaaatatc aatattatcc cagtcagttt tatttaaatc atcaaaagca gttgttttga
     4561 ttgtctgatt tccggcaatg atctgatcat ggccgggaat gaccgcggat tgaaacggac
     4621 catagaccga atcatattgg agcaggtaag ccagcattgc cggctgtttt ttgcgggtgt
     4681 tgatctggac aatatcaaag ctgtttttcg tcaaagcctg cctggtgaag atccggccaa
     4741 tccggccaaa accgttcagg ccgattctta gttttttcat atgccactat tataacacat
     4801 tgtcttaact ggtaaaattt tagtataatt aatgtggaaa atgttaaaaa atatggaaaa
     4861 gcatggatga gttaagtccc atgagtttta atttatgtca aatttagttg ttaaaagttt
     4921 agtttatata gctttaacct gcggtttttt gttttttatt tacgctgtcc ggtactattt
     4981 gataattatt tcagttttgc tgaagcaccg cggcaaaaga cgtcccagcg ctcttcagaa
     5041 aatttcgcca attaatctaa agagctgctg cggtttgttg tatggagaca atattgcttt
     5101 ccgctgcaat tttaaccggc acaatcaccg caaaggccgg catggccttt tacagcgggt
     5161 tgattatcta agtttgccca aagcgccgtt tatctccgtt cacattccat tttataatga
     5221 aagtaaaaaa gttattgacc gggttttggg agccagttta aatttggact atgaccatta
     5281 tgaaataatc gtctgtgatg acagcaccga cggcagtctg aattatatta aaaagaaatg
     5341 gggacgaaaa aaaggtttga aaattttaca tcgggacaac cggattggat ataaaggggg
     5401 cgctttaaag aatgctttga ggcggatgga ttcccggact gattttgtgg ttgtttttga
     5461 cgctgatttt atcccctatc cggatactct tcagttattt ttgaaatatt ttattattgc
     5521 caacaacggt tattttgatt ttaaaaaatc aaaaattggc gccatccagg gttatcagtg
     5581 gcacgtttta aataaagatg aaaactggat taccaaaggg atttatactg aatttggcgg
     5641 cagttatgtt tttgaccgct atggcgcgga aattttttct ggctttaagc aaattgccgg
     5701 ctcggttttt atggttcggg ccgatctgtt aaacaaaata ggctgggacg acagcattac
     5761 tgaagactgg gagctgactt tgaatttata ccagcggggc tacaaggtaa tttacacccc
     5821 gcatattcag acgccggctg aatgcgttaa tactctttca aaattaagaa agcaaagaac
     5881 gcgctgggcc gaaggccata cccgggatgt ccggaagcat ttttttaata tcttattttc
     5941 cggaaatgtt aactggcggg agaagctgga gtttttattt ttatctttgt tttacttaca
     6001 gtcagttttc ttcgtggtcg ggacgattag ctggctggct gttgaatttt tatttcccta
     6061 cactaaactc ccctggtgga cggcaatgct gggctggtcg ctggtcttta ccaacttttt
     6121 ggctttgccg ctggttaatt tagccgcttt gtttttacaa cggtctgata aaaaagatgt
     6181 tttaggaatt ttatctttca ttgtcttagg ctggtatttg gcgccgtttc agacctgggc
     6241 cgctttaaaa ggcctgctga ttgacaggcc cagttactgg caccggacgc ccaaatcggg
     6301 ccggttaagc cggttattca gacgaaaaaa acagcctgaa tattttccgg ccggcgaata
     6361 tatcagagcc tttttttcta aaagtaaaaa atttaaattt cagccgggcc attggctggt
     6421 tttgggcttg ctgtattttt tgtttgtttt tactctggta tttgttttat acagcggcac
     6481 tatcaagctg gcccgggcca atccggatga attttatttc caccggacag cggccggcgg
     6541 cgttcagcca agcggcaaaa acatgaattc agtcccgccg gctattcaga ccgcctttgg
     6601 cgtcggcgac ggcgcttatt atttttattc gggacaatat ccagccggag cgacggatac
     6661 ggtcattgct ccgggcaatt atattcagac aatctggata gaggccggca gctctttcag
     6721 ttttaattta gacggggagg tttctttgtg cgacccgaac ggcggcaact gtcagacaat
     6781 taccagcggg tcctgttcag ttgctgtcag cgccgggact aatcaatcca gttgtgattt
     6841 gggggctgga caagaacaaa caattgcttc agccaatccg caaataatca aaattgctct
     6901 gatctttaac ggcctgtctg atgggaattt tttgtatgac ggagttaacc agtcgtcagc
     6961 catgagttcg ccaacagtca ccgtgcccga aggggttatc tggttcagcc cggttataat
     7021 cggtttgtta tttttgatta agcctgtcct aagaagaaag caatatgtct aattttcaaa
     7081 ctttggaaaa gcaaaaatat aatttaggga cgattttgct ggtggcgacc gctttaattc
     7141 tattgctgct gccgttcgtg gcgactttta atgaattttt aaccagcctg gccgagaaag
     7201 tttatttgtt ccgctggatt gaaaatgtca ttgtgccgtt tgaatcgcgc tgggtggttg
     7261 gtttgttgtc ttttttggga attgagtcaa ttgccaccaa tagttctgtt tttttgactg
     7321 aaccggccgg agttggtctg aatgttttta tcagctggaa ctgcattggc tggcaaagtt
     7381 ttattttatt aattttcact tttttaacga tgcttcaggg tgttggttca gggtttcaaa
     7441 aaatgaatgt tgttttgttt gggattattg gcaccttttg ggttaatctg atcagaatag
     7501 cggccgtgat tttactggcc aaattttggg ggctgaaatc agccatcctt tttcatgatt
     7561 acgccggcaa tttgatggtt attgtctggc tcttggtttt ctggctgatt tcctttaaat
     7621 atttgctgat ttcccgggct atcctggaca aagcccaaaa atttgtttat aatgcttaga
     7681 aattcgcggg cgtcgtataa tggctattat gtcagccttc caagctgaag acggcggttc
     7741 gattccgctc gcccgctcca gttacaagtt aaaagtaata agtaaaaagt aagaagtgga
     7801 agtaataggt aaaa
//
LOCUS       DASWHZ010000093         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1688367, whole genome shotgun sequence.
ACCESSION   DASWHZ010000093 DASWHZ010000000
VERSION     DASWHZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688367"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1020
                     /gene="recO"
                     /locus_tag="VGA49_02085"
     CDS             265..1020
                     /gene="recO"
                     /locus_tag="VGA49_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9664590.1"
                     /translation="MTYKTSGIILKKQDWRDYDRLFTFYTKDFGKISAVSRGVKKIKS
                     KLAGNLQELAVIDLFIINGRRVDQLGGAVIDKNFLRIKANLDLINATLSSLELVDQLT
                     KEKLADPDIFSLLEDFLSAVNSINQPTGLKAVVLSQLFNFKFFDLLGYRPELYNCLEC
                     KNKISSGVNYFDYSGGVVCQNCQPQNLKLISKEAIKILRFSLDHNLGDYLKVNINKNL
                     DLELISIIDNFMKFRLDKELKSRAYIMNLVMSH"
     gene            1185..1880
                     /gene="pyrF"
                     /locus_tag="VGA49_02090"
     CDS             1185..1880
                     /gene="pyrF"
                     /locus_tag="VGA49_02090"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9664591.1"
                     /translation="MSDNPIIVALDGLDKDRALALAQQLSQTGCGFKVNSLLRREGRG
                     LLEDLSIFGLVMADFKLHDIPNTVVNDVSDLSQTLCEGDFATVHASGGIDMMSAAVKE
                     ASGKFQLLAITVLTSISPVQCELTFGCPPAIAVIKLARWAALARMDGIVCSPRELKEL
                     SRLAELRHLKKVTPGIRPEWYQNQTDDQQRVMTPKQAIDFGADYIVMGRPITTASDPL
                     EAMQRTLEEIRKG"
     gene            2000..>2322
                     /locus_tag="VGA49_02095"
     CDS             2000..>2322
                     /locus_tag="VGA49_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664592.1"
                     /translation="MKKLIIFVVMVVSLTAGCSSVDFLEYRLEKMNWALNKARDHLDQ
                     AEESFQEQKATLAETQKAYQVYLEVQAEVEQAYLESFAPAAARWLLDGHRRLADAYQE
                     SIQRQ"
BASE COUNT          650 a          449 c          588 g          635 t
ORIGIN      
        1 taaagacgtt aaaacagaag atgaaagatt tacctcaaag atggttgaaa gtttgcgaaa
       61 agaagctgac ggcggcagtc tggcggagaa agattcgatt gaagcgatgt taatgctgga
      121 aagttatctt gcaaaaactg agcaaccaaa aactgagaaa cagagaaact aaagaacaaa
      181 agaacaaaag aacctatggt tgcttagttg ctcagttcct cagttgctca gtgcctaaat
      241 tcctcagttt ctaagtttat atctatgacc tataaaacct ccggcattat tttgaagaaa
      301 caagattggc gggattatga ccggctgttt actttttata ccaaagattt tggcaaaatt
      361 tcagcggtct cccggggcgt caagaaaata aaaagcaagc tggccggaaa tttacaagaa
      421 ttggcggtaa ttgatctgtt tattatcaac ggccggagag ttgaccagct gggcggagcg
      481 gtcattgata aaaatttttt acgaattaag gcgaatttgg atctaattaa cgcaacttta
      541 tcaagcctgg aactggttga tcagctgacc aaagagaaac tggccgatcc ggatattttt
      601 agtctgctgg aagatttttt gtcagcagtt aattcaatta atcagccgac cggattgaaa
      661 gccgtggttt taagccagct gtttaatttt aaattttttg atttgctcgg ctaccggccg
      721 gaactctata actgtctgga gtgtaaaaat aaaattagtt caggtgtcaa ttattttgat
      781 tatagtggcg gcgtagtttg tcagaattgt caacctcaaa atttaaaatt aatatccaaa
      841 gaagccatta aaattcttcg cttcagcctt gatcataatt taggagatta tttaaaagta
      901 aatattaata aaaatttaga tttagagctg attagtatta ttgataattt tatgaaattt
      961 agactggata aggagctgaa aagccgggct tatattatga atttagtcat gagtcactag
     1021 tcattagaac ccctaatgac tgctcgtccc gcccttcgtg aagggcgggg ttgactgttg
     1081 actagtgact atgatatttt aataaaaatc tgtcagagat ggtctttgac aactgaacga
     1141 agcgttcctg tcaggaacgt tgtttgttat agaggagtcc tgaaatgtct gataacccaa
     1201 tcatcgttgc cctggatggc ctggacaaag accgggctct ggcattggct caacagttga
     1261 gtcaaaccgg ctgtggtttc aaagtcaact cacttcttcg ccgtgagggt cggggactgt
     1321 tggaggactt gtctattttc ggcctggtca tggccgattt taaacttcac gatattccta
     1381 acacggtcgt caacgacgta agcgatttat cccaaacatt gtgtgagggc gattttgcta
     1441 ctgttcacgc cagcggcggc attgacatga tgtcagcggc ggtcaaagag gcctcaggta
     1501 agttccaact gctggccatc acggtcctga cttcgattag tcctgttcag tgcgagctga
     1561 cttttggctg tccgccggcg atagccgtta tcaagttggc ccgctgggcg gctctggcca
     1621 ggatggatgg tatcgtttgt tctccgcggg agctgaagga actgtcccgg ttggcggaac
     1681 tgcgtcatct aaagaaagtc acgccgggta ttcgaccgga gtggtatcag aatcagactg
     1741 atgatcaaca gcgggtgatg acgccaaagc aggcgattga cttcggggcg gactatattg
     1801 tcatgggacg accgatcacc acagcgagtg atcctttgga ggcgatgcag cggacgcttg
     1861 aagaaatcag gaagggctag gagaactacg ccggttctgt aatggaatcg gtttttttat
     1921 tatttaataa ttgacattca tgccagaatg tgattagata gtggtgccta aaataccagt
     1981 cctttgaaag gaggaagccg tgaaaaagct gatcattttt gtggtgatgg tggtcagcct
     2041 gacggctggc tgttcatcgg ttgactttct ggaatatcgg cttgaaaaga tgaactgggc
     2101 gcttaacaaa gcccgggatc atctggatca ggctgaagaa tcttttcagg agcagaaagc
     2161 gactttggct gaaactcaaa aagcttacca ggtttatctg gaggttcaag ccgaggttga
     2221 acaggcctat ctggaaagct ttgccccggc ggcggcccgg tggctgctgg acggccaccg
     2281 acgcctggct gacgcttacc aagaatcaat tcagagacag ca
//
LOCUS       DASWHZ010000094         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2437424, whole genome shotgun sequence.
ACCESSION   DASWHZ010000094 DASWHZ010000000
VERSION     DASWHZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437424"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="mutL"
                     /locus_tag="VGA49_02100"
     CDS             complement(<1..1058)
                     /gene="mutL"
                     /locus_tag="VGA49_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX9664593.1"
                     /translation="MKIKALSQDLINKIAAGEVVERPASVVKELLENSLDAGSNQIAV
                     EIIKGGQDLIKVSDNGQGMTREDAELAVQRYTTSKIAELDDLFNINSFGFRGEALASI
                     ASVSRFKLQTKIDQELVGAEIEIIDGETIINEIGWETGTTVTVQNLFFNVPARQKFLK
                     TQSTEFNHILDLFSGYALLYPDISWKLIHNDKTILNLPKAKDWLTRIEAVLGADIARE
                     LLPVAFDGWEIRIKGFVAKPALSKSNSKSQYIFVNNRPVRDYLISMAVRDAYTTLIPR
                     GEYPVFVLKIDIDPGLVDVNVHPRKSEVKFLDTRLIYRAVYNLINSVFYSGDQPILSE
                     DDISKNPALQKPMLSQVS"
     gene            complement(1196..>2978)
                     /gene="mutS"
                     /locus_tag="VGA49_02105"
     CDS             complement(1196..>2978)
                     /gene="mutS"
                     /locus_tag="VGA49_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9664594.1"
                     /translation="DQATIRNLEIFNTLLEGKHQGSLLSVIDRTETAMGGRLLRSWLL
                     QPLINKDKIDQRLSAVAELTDQNQWRQTIADYLKKVADIERLAGRIGCQRATARDLIA
                     LKSSLEIIPRLKEILKQARSDFLVQTFNQLAEHQSAVDLIGRAIVDEPPLSIQAGGLI
                     KDNYDQELDSLREIAYSGKDWIRKMQAEEIERTGINSLKVKFNKVFGYYIEISKTNLD
                     SVPENYIRKQTLVNAERFITPELKEYEEKVLGAEDKIKQLEYNLFLEIRNKLSEHLPA
                     IQKSARLLARLDVLVSFADLALANNYCRPVITDDDQIIIKDGRHPVIETINSDPYIPN
                     DSCLNSQDHLLILLTGPNMSGKSSYLRQVALIVLMAQIGCYVPAKEASLSLVDRIFTR
                     VGASDNLAAGCSTFMMEMQEAANIINNATDKSLIILDELGRGTSTYDGVSIAWAVVEY
                     LHNHLKAKTLFATHYHELIEAAESLSKAQNYSVAVKENKTEGVIFLRKVIKGGIDKSY
                     GVEVAKLAGLPQDLIMRSLQILHKLENGHLTNGQDKQKTLPLMPAKKEEPLAAKLKNL
                     DLENMTPLEALKKLEELRQEAQGTRQG"
BASE COUNT          786 a          715 c          630 g          847 t
ORIGIN      
        1 tgactcacct gggaaagcat tggtttttgc agggccggat ttttagaaat atcatcttca
       61 gataaaatcg gctgatcgcc tgagtaaaaa acagagttaa ttaaattata aaccgcccgg
      121 taaatcagcc gggtatctaa aaatttaact tcgcttttcc ggggatggac attgacatca
      181 accagtcccg gatcaatatc aatttttaag acaaaaaccg gatattcccc cctgggaatc
      241 agcgtcgtat aagcgtcccg gacggccatt gaaattaaat aatcccggac cggccggtta
      301 ttgacgaaaa tatactgact tttggaattt gatttggaca aagccggctt ggcgacaaat
      361 cctttgatcc ttatctccca gccgtcaaaa gcaaccggca gcagctcacg ggcgatgtca
      421 gctcccagga cagcttcaat cctggtcagc cagtctttgg cttttggcaa atttaaaatg
      481 gttttatcat tgtggatcag cttccagctg atgtccgggt aaagcagggc gtagccgctg
      541 aataaatcaa gaatgtgatt gaattcagtt gactgggttt ttaaaaattt ctggcgggcc
      601 gggacattga aaaataaatt ctgaacagta acggttgtcc ccgtttccca gccaatctcg
      661 ttaatgattg tttcgccgtc aataatttct atttccgcgc caaccagttc ctggtcaatt
      721 tttgtctgaa gcttaaaccg ggaaacagaa gcgattgaag ccagggcttc gccccgaaaa
      781 ccaaaactat tgatattaaa taaatcgtcc agctcggcaa tcttgctggt agtgtagcgt
      841 tggacagcca gctcggcgtc ttcccgggtc atgccctggc cattgtcgct gactttaatc
      901 aaatcctggc cgcctttgat aatttcaacg gcaatctggt ttgacccggc gtccagagag
      961 ttttcaagca actctttgac aactgaagcc ggccgctcaa ccacctcgcc ggcggcgatt
     1021 ttattgatca aatcctggga tagcgctttg attttcatag caagtaaagt ctagcaattt
     1081 aaactaattt cttcaatcct agggtagatg aaggttgatg agggttgatg agggttgatg
     1141 agggtagatg agggttaatc cttaatcaac cttaatatac cttaataaac tctaatcaac
     1201 cttgtctggt gccttgtgcc tcttgcctaa gttcttctaa cttttttaac gcctctaagg
     1261 gcgtcatatt ttccaaatcc aaatttttca atttagccgc cagcggctct tcttttttag
     1321 ccggcatcag cggcaatgtt ttttgtttat cctggccatt tgttaagtga ccattttcca
     1381 gcttatgcaa aatttgaagc gaccgcatga ttaaatcctg gggcaggccg gccagtttag
     1441 cgacttccac gccgtaactt ttatcaatgc cgcctttaat aacttttcgt aaaaaaatca
     1501 cgccttcagt tttattttct ttaacggcca ctgaatagtt ctgggctttg gacaaggatt
     1561 cggccgcctc aatcagctcg tggtaatgag tggcaaataa agttttggct tttaaatgat
     1621 tatgtaaata ttcaaccacc gcccaggcaa tgctgacgcc gtcataagtg ctggtccccc
     1681 ggccgagttc atcaagaata atcaggcttt tatcagtagc gttattaatg atattggccg
     1741 cttcctgcat ttccatcata aaagtggaac agccggccgc cagattatca gaggcgccaa
     1801 cccgagtaaa aatccggtca accaggctta agctggcttc tttggccgga acataacagc
     1861 cgatctgggc cattaaaacg attaaagcca cctgccgcaa atagcttgat ttgccggaca
     1921 tattgggccc agtcagcaaa atcagcaaat gatcctgact gttcaaacag ctgtcgtttg
     1981 ggatatacgg gtcggaatta atggtttcaa ttaccggatg gcggccgtct ttaatgatga
     2041 tttgatcatc gtcagtaatc accggccggc aataattatt ggccaaagct aaatcagcaa
     2101 aacttaccaa aacatccagc cgggccagca accgggctga cttttgaatg gccggcagat
     2161 gttccgacag cttatttctg atttccagaa ataaattata ttccagctgc ttaattttat
     2221 cctccgctcc caaaactttt tcttcgtatt ctttcagctc cggagtgata aaccgctcgg
     2281 cgttaaccaa agtttgcttt ctgatataat tttcaggaac cgaatcaaga ttagtttttg
     2341 aaatttcaat atagtaacca aaaaccttat tgaatttgac tttcaaagaa ttaatgccgg
     2401 tccgctcaat ttcttcggcc tgcattttcc tgatccagtc tttgccggaa taggcaattt
     2461 cccttaaact gtccagctcc tgatcataat tatctttgat taggcctcca gcctgaattg
     2521 acagaggcgg ctcgtcaaca atcgcccgcc cgatcaaatc aaccgctgac tgatgctctg
     2581 ccagctgatt aaatgtctga actaaaaagt ctgaccgggc ttgttttaga atttctttaa
     2641 gccggggaat gatctctaaa ctgcttttca gagcaatcaa atcccgggca gtggcccgct
     2701 ggcagccgat ccggccggcc agccgctcaa tatcagccac tttttttaaa tagtcagcga
     2761 tagtctgtcg ccattgattt tgatcagtca gttcagccac ggccgacaac cgctgatcaa
     2821 ttttatcttt gttaatcaaa ggctgaagca gccaggagcg cagcagccgg ccgcccatgg
     2881 ctgtctctgt ccggtcaatc accgacagca aactgccctg atgtttacct tccagcaaag
     2941 tgttgaaaat ttccagattg cgaatcgtcg cctgatcc
//
LOCUS       DASWHZ010000095         7931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2792736, whole genome shotgun sequence.
ACCESSION   DASWHZ010000095 DASWHZ010000000
VERSION     DASWHZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792736"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..74)
                     /locus_tag="VGA49_02110"
     tRNA            complement(1..74)
                     /locus_tag="VGA49_02110"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Asp,seq:gtc)
     gene            complement(230..301)
                     /locus_tag="VGA49_02115"
     tRNA            complement(230..301)
                     /locus_tag="VGA49_02115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Pro,seq:cgg)
     gene            complement(306..377)
                     /locus_tag="VGA49_02120"
     tRNA            complement(306..377)
                     /locus_tag="VGA49_02120"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Val,seq:cac)
     gene            1502..1819
                     /gene="rpmE"
                     /locus_tag="VGA49_02125"
     CDS             1502..1819
                     /gene="rpmE"
                     /locus_tag="VGA49_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9664595.1"
                     /translation="MKSQIHPKYYEKAKVSCACGTNFEIGSTVPEISVELCSQCHPFY
                     TGKMKLVDTAKRVDRFKARAAKQEKLAADRKGKKAKKAARQVRAVEKKEKTEAETVVK
                     AEQ"
     gene            1879..2919
                     /gene="prfA"
                     /locus_tag="VGA49_02130"
     CDS             1879..2919
                     /gene="prfA"
                     /locus_tag="VGA49_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX9664596.1"
                     /translation="MKNEIKKIGDKIKLLEQELNSPDIVKNPDKLKAVSKEYNQAKEL
                     LDFYNQLGKINQAIAEIQKTIDQETDRELIDLAKEELPQLETKKAHLEKKLKLALTPK
                     DPLDQKNVIMEIRAGTGGDEAALFAANLFRMYSRYAENKKWKTSILSSNRIGLGGFKE
                     IIFEINGSDVYGDLKYESGVHRVQRVPETEKSGRIHTSAATVAVLPEAEEVDIKIDPK
                     DLKIDTFCASGHGGQSVNTTYSAVRITHLPTNLVVSCQDERSQQQNKEQAMTILRSRL
                     FDLEQDKKQKAEAAKRKQLVKTGDRSEKIRTYNWPQDRITDHRIKQNWHGISTILDGG
                     IDKIITSLKKTE"
     gene            3141..3992
                     /gene="prmC"
                     /locus_tag="VGA49_02135"
     CDS             3141..3992
                     /gene="prmC"
                     /locus_tag="VGA49_02135"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9664597.1"
                     /translation="MTIQKLLTTSYKKLKKAEIDSANLDAVILLYHVLRKPKAYLFTH
                     PEKLLTSKQIENYNKLIQRRLKHEPIAYLTGHKEFYGFDFLVNKNVMIPRPETELLID
                     QVIKTGNKIFNIVDVGTGSGCVSVTLAKKLSRAKILTTDISKKALALAKKNAAKHQVS
                     KKINFYQGNLLEPVKNQKIDLLVANLPYLSKKEYLHSPFARELSFEPTAALTDRADGL
                     SLFQEIFDQLGSFSTLKKSLKYLALEIGYEQAADIKKLVKKVFPKATMTIIKDFCGFD
                     RVLIAKI"
     gene            4007..4591
                     /locus_tag="VGA49_02140"
     CDS             4007..4591
                     /locus_tag="VGA49_02140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9664598.1"
                     /translation="MTLIIYAHPPVEGFCSHILKEIQDDLDCRKIKYEVIDLYKIDYD
                     PVLKTNELYTAGNREISQQNLKFQEKIKTADKLILIYPVWWNSSPAIIKGFFDRVMTP
                     RFAYKFYKKFGIGYPVKLLKGKKAAVFQTSGSNKLITLVINRNRAKKITPRDILGFSG
                     IKARVFHLGRANRLTDDKLVEIKKLVKKGLNWLY"
     gene            complement(4563..5666)
                     /locus_tag="VGA49_02145"
     CDS             complement(4563..5666)
                     /locus_tag="VGA49_02145"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX9664599.1"
                     /translation="MKKKRLKLNRKRLKARKLNNPAMAEFKVFEKKNNLIIGLLAIII
                     IAAVSVVIFIGLNKPADPAGLLPAEEEARAQKPSELFNDQISLAVTIENMFEVRPQDG
                     LSRAKLVIEAPVEGGITRFLAFYDGSENLSRIGPIRSARPYFLDWAEEWRALYVHVGG
                     SPEALNLIKKNNIFNLNQFFESWYFWRSARRVAPHNVFSSSELLKQALTDKDWQEPEG
                     FERLIYKDDLGAEDRPDQVNDIVIDYSTEPYRVIWKYDREANSYLRYQGGQPHLDAAG
                     QQLKVKNVLIQFAEMRVIDNEGRRYIKTIDNNIGILYRDGQVMEINWEKKDRSDRTKF
                     YDLTGPEIEFNRGITWIEVVPRDLLNTTSSSLF"
     gene            complement(5608..6324)
                     /locus_tag="VGA49_02150"
     CDS             complement(5608..6324)
                     /locus_tag="VGA49_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX9664600.1"
                     /translation="MESTSLSVATRQTTGKKVKDLRKKGLIPGVVYGPDLKPRNLKID
                     YNSFEKIYDRGGYSILIDLKIDDQPESVKVLITELQKDPVTDKFTHVDFHQVRLTEKL
                     ITSVKLKFIGQSDAVKGLGGTLVTNLSDLEIRCLPQDLVSEIEVDISTLKTFEDVVRI
                     KDLQIPANIEVMNNPEASVAAVVAPRTEEELKALEEAPEEKVGEVEVEGEQSAEGGEA
                     AGEEEKAEVKPEAAEGQKAK"
     gene            complement(6326..6673)
                     /locus_tag="VGA49_02155"
     CDS             complement(6326..6673)
                     /locus_tag="VGA49_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664601.1"
                     /translation="MLEHEDLFQLLPEGLRFVSSCPICGVKFNPIQARIIDEKEDAHL
                     IHIQCKKCHSCIIALIYTADVGVTSIGLITDLTAEDVIKFKSTDSIGSDDVIEVYRLL
                     ENSRPSQFIKLLN"
     gene            complement(6715..7815)
                     /gene="rodA"
                     /locus_tag="VGA49_02160"
     CDS             complement(6715..7815)
                     /gene="rodA"
                     /locus_tag="VGA49_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX9664602.1"
                     /translation="MIARYIDHFKKFDWILFIVVFLLISFGLAAIYSVNLSLEGNNFL
                     NFKKQLIFAGIGLALLFIFSFVDYRIFRSYSHIFYAVGIIALLGVILLGVKIRGTRGW
                     YQLGGFTFQPVELVKLFSIIFMAKYFSTWARSLDKFRHIILSGLGVFVYFMLVAFQPD
                     FGSAIILFLLWLGSVLVIGIKKSHLAVIGLILVLISTFSWFFLLAGYQQSRIMTFLNP
                     AADPLGRGYNITQSTIAIGAGQLFGRGLGFGSQSQLKFLPESQTDFIFAVIAEELGFA
                     GVALILVFFGLLLFRLFRVFSLVRNDFELFLVLGIMILFFSHILINVGMNLGIAPVTG
                     ISLPFLSYGGSFLITSLLMIGIVQNIIISSKA"
BASE COUNT         2501 a         1873 c         1512 g         2045 t
ORIGIN      
        1 tcggggatac cggactcgaa ccggtggcct ccccgatgga catcggggcg ggctaccaac
       61 tgcgccaatc cccgtcgata aaaaccaata actagctacc aataaaccaa atcggggtac
      121 cgggatttgc cccgccggac gaccgcaaag cggtcttggc ggggtcccgc cataggcggg
      181 aaacccggct gacaaaaaat ccatccgata gccttgtctt ttaaaaattt cggggtaccg
      241 ggatttgaac ccgggacctc gtcgtcccga acgacgcgcg ctaccactgc gctatacccc
      301 gtctatgggc actgcaggat ttgaacctgc gacctcttcg atgtgaacga agcgctctac
      361 cgctgagcca agtgcccatt atcgattcta attggcatca cgtattggtt gtaaacaaaa
      421 aaaccttcac caaagagtaa ttttaggctc tgagacaacc gggcgacttt ttaaaacact
      481 actgaccaag ttggaaaacc cgaccaaagt aattttaaca agttatcgcc gcgtagaatc
      541 cgcttggcca aaccgcggtt tagccaaatt tggatcctct caatcaccca tttgatccca
      601 aattacatcc ttttagtgaa gttattatat tatatcagat ttagcaaggt cgatcaaaac
      661 ggccagcgtt taaaaatctc ttacctgaaa ttgttctgat tcgatcttat tcgctcatta
      721 tttgcttcaa ataaaaaacc accgcctcca aaagagtggt ggttgacgcg accccctgaa
      781 aagaacttag ttatgccccg gctaacaaag ggctgactga aagccggcga ctttcgcctt
      841 gcctcctgga cttacagtcc ggacaaaggg tgaaaacctg atgcttcgac ttcttgcctt
      901 gctgctgctt aacccagccg gcttctgtct cgactagttt gcaggaagcg caaacggtta
      961 tccgcttgat tgccgctggc tcttgcgctt gcattctggc cgaccgggac ggtagccccc
     1021 ttgctggcat tgttctgaaa agaaccatct tccctccttt ggttaactgg aacagagcta
     1081 acagccagat aactaactat caactcttca ccaacaaacc cttagcctcc cctcttatcc
     1141 ttcttatctc tcttatcccc cttatccctt ttataaaaaa tctagccgct ttggaagcgg
     1201 ctagcgcagg ttactaaatg ttttagaact tactctgcaa aactagccgc tgatccaatc
     1261 aacataaaac caaaaatggc ccgaataatc atgtcgaggg ccagtttttt atagttttgc
     1321 aaggcggcta gagacatctt cattagaatt agattaaaac taattaaaag ttatcatagt
     1381 cgaaaaagtc tgtcaaatta aatatccaca accctaaaaa acgcgcctaa acttgccttt
     1441 ttttaaattt aatgctatca tttttaaatt aaccaataat tagcttttat tcgcggaaga
     1501 tatgaaatct caaatccatc caaaatacta cgaaaaagcc aaagtttcct gcgcctgcgg
     1561 aaccaatttt gaaattggat caacagtccc tgaaattagc gttgagctct gctcccagtg
     1621 ccatcctttt tataccggga aaatgaagct ggttgacacc gccaaacggg ttgaccggtt
     1681 taaagccaga gccgctaaac aggagaaact ggccgctgac agaaaaggta aaaaagccaa
     1741 aaaagccgcc cgccaggtca gggcagttga aaaaaaagag aaaactgaag ctgaaactgt
     1801 tgttaaagcc gaacagtaaa ctaaataaat taaaactcaa atcccctgtt gctggggatt
     1861 ttgtgttaaa ataactttat gaaaaatgaa ataaagaaaa ttggagacaa aatcaaactg
     1921 cttgaacaag agctgaacag tcctgatatt gtcaaaaatc cagataagct caaagccgtt
     1981 tctaaagaat ataatcaagc caaagaactg ctggattttt ataatcaact gggaaaaatt
     2041 aaccaggcga tcgctgaaat tcaaaaaact attgaccagg aaactgatcg ggagttaatt
     2101 gatttagcca aagaagaact gcctcagctg gaaactaaaa aagcccacct ggaaaaaaaa
     2161 ctgaagctgg cgctgacccc gaaagacccg ctggatcaaa aaaatgttat tatggagata
     2221 agagccggta ctggcgggga cgaagccgcc ctgttcgcgg ccaacctttt tagaatgtat
     2281 tcccgctacg cggaaaataa aaaatggaaa accagcattt tatcatcaaa ccggatcggc
     2341 ctgggcggct ttaaagaaat catttttgaa ataaacggca gcgatgttta cggcgatttg
     2401 aaatatgaaa gcggcgtcca ccgggttcag cgggtgccgg aaactgaaaa aagcggccga
     2461 atccacacct cggcggccac ggtggcggtt ttgccagaag ctgaagaagt tgacattaaa
     2521 attgatccca aagatttaaa aattgatacc ttttgcgctt ccggccacgg aggccagagc
     2581 gttaacacca cttattcagc ggtccgaatc acccatttgc cgacaaacct ggttgtttcc
     2641 tgtcaggacg aacgctccca gcagcaaaac aaagaacaag ccatgaccat tttgcgttca
     2701 cgattatttg atctggaaca ggataaaaaa caaaaagctg aagccgccaa aagaaaacag
     2761 ctggtcaaaa ccggcgaccg gtcagaaaaa atcagaacct acaactggcc ccaggaccgg
     2821 atcactgatc accggattaa acaaaactgg cacggaatca gcactatttt ggacggcggg
     2881 attgataaaa tcattacttc tctaaaaaaa acggaataaa gaattagaca attcattaat
     2941 tactgattac gaattacgga ttttaataag tcaaaagtaa gaagtaaaaa gtaaaaaatt
     3001 tggaatattt tattttaaat aactttttcc tccagaggcg gatcagcccc tgactgaaac
     3061 ttattacttg aactttttat tttttccgcc taaggcggat ccgcccttgg cggatacttt
     3121 taccttttta cttaatcaaa atgactatcc agaagctatt aactacttcc tacaaaaagc
     3181 tcaaaaaggc cgaaattgac tccgccaacc tggacgccgt catcttgctc taccacgtcc
     3241 tgcgtaaacc taaagcttac ttatttaccc atccagaaaa gctattaact tcaaaacaga
     3301 ttgaaaatta taataaactc atccagcgaa gactcaaaca cgaaccaatc gcttatctaa
     3361 ccggccataa agaattttat ggctttgatt ttttggttaa taaaaacgtg atgatccccc
     3421 gtccggaaac tgaactgctg attgaccagg ttataaaaac aggcaacaaa atttttaata
     3481 tagttgatgt tggaaccggc agcggctgcg tttcagttac tctggccaaa aaattatccc
     3541 gggccaaaat tttaacaact gatatttcca aaaaagcttt ggccctggct aaaaagaacg
     3601 ccgccaaaca tcaggtctct aaaaaaataa acttttatca gggcaattta ctggagccgg
     3661 ttaaaaatca aaaaattgat ctgctggtcg ccaacctgcc ttatctatcc aaaaaagaat
     3721 atcttcactc gccttttgcc agagagctaa gctttgagcc gacagccgct ctgaccgacc
     3781 gggctgatgg tttaagctta tttcaggaaa tatttgatca gctcggctct ttttcaactt
     3841 taaaaaaatc cttaaaatat ctggcgctgg aaatcggtta tgaacaggcg gctgatataa
     3901 aaaaactggt taaaaaggtt tttcctaaag ctacaatgac aatcataaaa gatttttgcg
     3961 ggtttgaccg ggtcttaatc gctaaaatat gatattataa aaaaatatga ctttaatcat
     4021 ctacgcccat ccgccggttg aaggcttttg ctcccatatt ttaaaagaga tccaggacga
     4081 tttggattgc cgaaaaataa aatatgaagt aattgatctt tacaaaatag attatgaccc
     4141 ggttctcaaa actaacgagc tttataccgc cggcaaccgg gaaatcagcc aacagaatct
     4201 aaaatttcag gaaaaaataa aaaccgcgga taaattaatt ttaatttatc cagtctggtg
     4261 gaattcttcc ccggccatta tcaaagggtt ttttgaccgg gtaatgacgc cccgttttgc
     4321 ttataaattt tataaaaaat tcggcattgg ttatccggtt aaattattaa aaggcaaaaa
     4381 agcggccgtc tttcagacca gcggttccaa taaactgatc actctggtga ttaaccgcaa
     4441 ccgggccaaa aaaataactc ccagagatat tctgggattt tccggtatca aggccagggt
     4501 gtttcatctt ggccgggcca accggctgac tgatgacaag ctggttgaaa ttaaaaaact
     4561 ggttaaaaaa ggcttgaact ggttgtattg agcaggtccc ggggaacgac ttcaatccag
     4621 gttatcccgc ggttaaattc aatttccggt ccggttaaat cataaaactt tgtccggtca
     4681 gaccggtctt ttttttccca gttaatttcc atgacctggc cgtcccggta aaggattccg
     4741 atattattat caatcgtttt aatataccgg cggccttcgt tatcaatcac tctcatttca
     4801 gcaaactgga taagaacatt tttaacctta agctgttgcc cggctgcgtc caaatgcggc
     4861 tggccgccct ggtatcttaa atagctgtta gcttcccggt catatttcca aataacccgg
     4921 taaggttcgg ttgaatagtc aatgacaata tcattaacct ggtccggccg gtcttcagct
     4981 cctaaatcat ctttataaat caacctctca aatccctccg gctcttgcca gtctttatca
     5041 gttaaggcct gcttcagcag ttcactggag ctgaaaacat tgtgcggagc cacccgccgg
     5101 gcgctccgcc aaaaatacca gctttcaaaa aactggttca aattaaaaat attattcttt
     5161 ttaattaaat ttaaagcttc cggactgccg ccaacatgaa catataaagc ccgccactct
     5221 tcagcccagt ccaaaaaata aggccgggcc gaccggatcg gtccgattcg gcttaaattt
     5281 tctgaaccgt cataaaaagc cagaaatctg gtgatgccgc cctcaaccgg cgcttcaatc
     5341 accagttttg cccggctcaa accgtcctgg ggcctgactt caaacatatt ttcaatggtc
     5401 accgccaggc tgatctgatc attgaataat tcagagggct tttgggcccg ggcttcttct
     5461 tcagccggca ataaaccagc cggatcagcc ggcttgttta aaccaataaa aataactact
     5521 gaaacagcag ctataatgat aatggccagc aggccaatta ttaaattatt ctttttttca
     5581 aaaactttaa actccgccat tgccggatta tttagctttc tggccttcag ccgcttccgg
     5641 tttaacttca gccttttctt cttcaccagc ggcctcgccg ccttcggcgg actgttcacc
     5701 ctcaacctca acttcgccaa ctttttcttc cggagcttct tccagggctt ttagttcttc
     5761 ttcggttcgc ggcgcgacca cggcggccac ggacgcttcc gggttattca tcacttcaat
     5821 attagccgga atctgtaaat ctttaatccg gacaacatcc tcaaatgttt tcagcgttga
     5881 aatatcaact tcaatttccg acactaaatc ctgcggtaga catctgattt ccaaatcaga
     5941 taaattggtc accagggttc cgcccaaacc cttaacggcg tcggactggc cgataaattt
     6001 cagtttgacg gaagtaatca gtttttcagt cagcctgacc tgatgaaaat caacatgagt
     6061 aaatttatca gttaccggat ctttctgcaa ttcagtaatc aaaactttga ctgattcggg
     6121 ctggtcgtca attttgaggt caatcaaaat actgtaacca ccccggtcat aaattttctc
     6181 aaaactatta taatcaattt ttaaatttct cggttttaaa tccgggccat aaacaacacc
     6241 cgggattaat cccttttttc ttaaatcttt aacttttttg ccggtcgtct gtctggtggc
     6301 gaccgataaa ctggttgatt ccatattaat tcaaaagctt aataaactga ctaggccggc
     6361 tgttttccag taaccggtag acttcaatca catcgtcgct gccaatacta tcggttgatt
     6421 tgaacttgat aacatcttca gcggtcaaat ccgtaatcag accgattgag gttacgccga
     6481 catcagccgt gtagatcagg gcaataatgc agctatgaca ttttttgcac tgaatatgaa
     6541 tcaaatgagc gtcttccttt tcatcaataa tccgggcctg aatcggattg aacttgactc
     6601 cgcaaatcgg acagctggaa acaaatctca acccttcggg caacaactgg aataaatctt
     6661 cgtgttccaa cataatttta aattaggatt attataacaa tttcttaaaa atagtcaagc
     6721 cttggagctg ataattatat tttggacaat gccgatcatc agcaggctgg taatcaaaaa
     6781 actgccgcca taacttaaaa aaggcagtga aatcccggtt accggggcaa tccccaggtt
     6841 catgccgaca ttaattaaaa tatgactgaa aaataaaatc ataatcccca gcaccaggaa
     6901 cagttcaaaa tcatttctaa ccagactgaa tacccggaac agccggaaaa gcagcagccc
     6961 aaaaaaaacc agaatcaaag ccacgccggc aaaaccgagc tcctcggcaa tgaccgcgaa
     7021 aataaaatct gtctgacttt ccggcaaaaa cttcagctgg ctttgcgaac caaaacccag
     7081 tccccggcca aaaagctgac cggcgccaat ggcaattgtt gactgggtga tattataacc
     7141 ccgtcccagc ggatcagccg ccggatttaa aaaagtcata atccgcgact gctgatagcc
     7201 cgctaaaagg aaaaaccacg aaaaagttga aattaaaact aaaatcaacc caatcactgc
     7261 cagatgcgat tttttaatgc caatgactaa aactgacccc agccataata aaaacaaaat
     7321 aatggccgag ccaaaatccg gctggaaagc caccagcata aaataaacaa aaacacccag
     7381 gccgcttaaa ataatatgcc tgaatttatc cagactgcga gcccaggttg aaaaatattt
     7441 tgccataaaa ataatggaaa aaagcttgac cagctcaacc ggctgaaaag taaaaccccc
     7501 cagctggtac cagccccggg tgcctctgat tttaacaccc aataaaatta cccccagcaa
     7561 agcaatgatg ccgacagcgt aaaaaatatg ggaatagctt ctgaaaatcc ggtaatcaac
     7621 aaaactgaaa ataaacagca gggccaaacc aatgccggcg aagattaact gttttttaaa
     7681 attcaaaaaa ttattgcctt ccagactcaa attaacgctg taaatcgccg ccagcccgaa
     7741 gctaatcaat aaaaaaacaa caataaataa aatccagtca aactttttaa aatgatcaat
     7801 atatctagct atcatttaaa tcttcgttaa aggtttttcg ggttagtagc ccgtatggat
     7861 aaaggtttgg ttttaggtgt agcagccggt atggtttttg gttttagttt taggcttttc
     7921 accaaaaccc a
//
LOCUS       DASWHZ010000096         4136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_196127, whole genome shotgun sequence.
ACCESSION   DASWHZ010000096 DASWHZ010000000
VERSION     DASWHZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196127"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VGA49_02165"
     CDS             complement(<1..220)
                     /locus_tag="VGA49_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9664603.1"
                     /translation="MVLFWLIPLLILGLIGWLIFVYNALIRGRNNVEESWADIDVQLK
                     RRYDLIPNLVETVKGYAKHEASVFEKVTQ"
     gene            345..1472
                     /gene="pilM"
                     /locus_tag="VGA49_02170"
     CDS             345..1472
                     /gene="pilM"
                     /locus_tag="VGA49_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX9664604.1"
                     /translation="MALFGPSKISYLGVDIGTSGIKLIELARQGGRAKLVTYGYSDKP
                     IALIKNEPAVTDINQAAGDLKNILKQSKTTTLSTIASLPGFAVFSSIISLPPMDKKEL
                     DQAIKNEAQKVIPRPIEEMVIDYKIFAKGQKEKISKSVSLNQTEDETETIIKAARPEE
                     VTKILLTAAPKELVEKYVAVFKKAGLNLLSLETEPFALTRSLIGADKSTVMIVDLGAV
                     TANISIVSNGIPIMSRAIDTGGLAITDAIQQSLNIKKDQAEQIKKDLSAAPMSAPGEQ
                     LPRAIENAVIPLINEVKYIFDIFQGENRPGDGQIEKIVLAGGSAYLPNLPDYISKAMN
                     IKVFIGDPWARVIYPEDLKPVLDEIGPRFATAIGLAMRNIE"
     gene            complement(1558..2001)
                     /locus_tag="VGA49_02175"
     CDS             complement(1558..2001)
                     /locus_tag="VGA49_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664605.1"
                     /translation="MSKKPEYPATFGPSMTEKGKVATEKRETEPQISHEEYFRQRSAI
                     LRKMESNRDELKRVIKEAKEKQIEEEFFKDLEKPAQDLLLNVRRYLKNEQPEVEATLT
                     RNKEWLEKVQKGEALTLDEKELEEKIDKAETYLKDINVFLEETRK"
     gene            complement(2041..2268)
                     /locus_tag="VGA49_02180"
     CDS             complement(2041..2268)
                     /locus_tag="VGA49_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664606.1"
                     /translation="MAEEAQDSLDGLEGDICAARIRADIFEEKLSEEELGSLVFRIKE
                     GERRETAVSWQEHQEQHNKGAVTLKPPHDQT"
     gene            2648..3289
                     /gene="clpP"
                     /locus_tag="VGA49_02185"
     CDS             2648..3289
                     /gene="clpP"
                     /locus_tag="VGA49_02185"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX9664607.1"
                     /translation="MTQSEIKQELNSLSLTSDGIRSQFLIPTVIEKTSHGERAYDIYS
                     RLLKDRIIFLGSPIDDNVANIVIAQLLFLENQDKEKDIKIYINCPGGSVTAGLAIYDT
                     MKYIKPDVMTICVGLAASMAATLLSAGAKGKRLCLPNSEILIHQVMGGAEGQATDIKI
                     RAERILKIKTRLNEILAEHTGQPIEKIENDTDRDYFMSAREAKDYGIVDKIID"
     gene            3772..>4136
                     /locus_tag="VGA49_02190"
     CDS             3772..>4136
                     /locus_tag="VGA49_02190"
                     /inference="COORDINATES: protein motif:HMM:NF023495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEX9664608.1"
                     /translation="MSTIYLIIIVVGVPLAAVFGYLIRKNIAGRQVDSAEAKAQNMLV
                     EAKNKQKELLLESKDKSLKIIEDAKKEAQERRQEISHLQSRLEKRESLFDQKLLDLEN
                     KNQKLNDKAAQIEEVKEQI"
BASE COUNT         1239 a          948 c          773 g         1176 t
ORIGIN      
        1 cctgggtaac tttttcaaaa actgacgctt cgtgtttggc gtagcctttg actgtttcca
       61 ccaaattagg aatcagatca taccgccgtt taagctgaac atcaatatca gcccaggatt
      121 cctcaacatt gttccttccg cggatcaggg cgttgtaaac aaaaatcagc cagccgatca
      181 gacctaaaat aagcagcgga attaaccaaa ataaaaccat attgtttgag ttaataatat
      241 tagtttaata tagatgaaaa atggactcct gtcaaaaaca ttttatacaa tatttttcaa
      301 atttatgcta tcataactta atacttaatc ttaaatctac ccttatggcg ttattcggtc
      361 cgtcaaaaat atcttatctg ggtgttgata tcggcacttc gggcattaag ctgattgaac
      421 tggccaggca gggcggccgg gccaaactgg ttacttacgg ctacagcgac aaaccgattg
      481 ctctgatcaa aaatgaaccg gccgtgactg acatcaatca ggccgccggt gatctgaaaa
      541 acattcttaa acaatccaaa acaaccaccc tgtcaaccat cgcttctttg ccgggctttg
      601 ccgtcttcag ctcaatcatc agcctgccgc caatggataa aaaagaactt gatcaggcca
      661 ttaaaaatga agcccaaaaa gttatccccc ggccgattga agaaatggtc attgattata
      721 aaatattcgc caaaggccaa aaagaaaaaa tttccaaatc ggtttccctc aatcagactg
      781 aagatgaaac tgaaacaatt ataaaagccg ccaggccgga agaagtcacc aaaattcttt
      841 taaccgccgc gcccaaagag ctggtggaaa aatacgtggc ggtttttaaa aaagccggcc
      901 ttaatttatt aagcctggaa accgaacctt tcgccctgac cagatcatta attggcgctg
      961 acaaatcaac cgtgatgatt gttgacctgg gagcggtcac cgccaatatc tcaatcgtca
     1021 gcaacggcat tccaataatg agccgggcaa ttgataccgg cggcctggcc attaccgacg
     1081 ccattcagca aagcctgaat attaaaaaag atcaggccga acaaattaaa aaagatttga
     1141 gcgcggcccc aatgagcgcg ccgggcgaac agcttccccg ggcaattgaa aacgctgtta
     1201 ttccgctgat caatgaagtc aaatatattt ttgatatttt ccagggagaa aaccggccgg
     1261 gcgacggcca aatagaaaaa atagttctgg ccggcgggtc ggcctacctg cccaatctgc
     1321 ctgactatat ttccaaagca atgaatatca aagtttttat cggcgatccc tgggcccggg
     1381 tgatttaccc ggaagattta aaaccagtcc tggatgaaat cggaccgcgg ttcgccacgg
     1441 ccatcgggct ggcgatgagg aatatagaat aaataagtcc aaagtccaaa gttcaaaatt
     1501 atttaatcaa aaaaccgttc cggcgatcag gaacggtttt tttgatttac taatatttta
     1561 ttttctagtc tcttctaaaa aaacattaat gtcttttaaa taagtttcag ctttgtcaat
     1621 tttctcttcc agttcttttt cgtctaatgt caaagcttcg cctttttgaa ctttttccag
     1681 ccattcctta ttacgcgtaa gtgttgcctc tacctctggt tgctcatttt taagataacg
     1741 tctgacattt aataacaaat cttgagctgg cttctccaaa tccttaaaaa attcttcttc
     1801 aatttgtttt tctttagctt ctttaattac cctttttaat tcatctctat tactctccat
     1861 ttttcttaaa atagccgatc tctgtctgaa atattcttca tgactgattt gcggttctgt
     1921 ttctcgtttt tcagtagcca ccttgccttt ttcggtcatt gaaggcccaa aagtcgccgg
     1981 gtattcaggt tttttagaca tatttttttg ttaattactt tttaaaaaaa tccccaaaaa
     2041 tcaagtttga tcatggggcg gcttcaaagt cactgcgcct ttgttatgtt gctcttgatg
     2101 ttcttgccag ctgacagctg tttcccgtcg ttctccttct ttgattctga aaaccaaact
     2161 tcctaattcc tcttccgaca acttttcttc aaaaatatcc gctcgtattc gcgctgcgca
     2221 aatgtctccc tccagaccat caaggctatc ttgggcttct tcagccatga caactcctct
     2281 caacaagcaa agtgcggaaa cttcctccac ctcaaatcta actaatttcc ccggctttgt
     2341 caattcttga caaatgattt tttatgtggt aaggtctaat gtccgttcag attaactaac
     2401 agcagtcacg acaagcatgg aaatttaact acaggatacc gcccggctcg cgcttggctt
     2461 ttcctggact aaattttctt gactgctgtt aattgatctg aatataaaaa acactcctaa
     2521 cttcagttca gagtgttttt atatggcgag ctgacttagt ttgacttgcc cttaatttca
     2581 tcttaaacta aatattacta ataaccaact accaaatact aactaccaac tactattatc
     2641 acatcctatg acacaatccg aaataaagca agagcttaat tccctgtcgc tcacgagcga
     2701 cgggatccga tcccagtttt taattccaac cgtcattgag aaaacttccc acggcgagcg
     2761 ggcctatgac atctactccc gtttgctgaa agaccggatt attttcctgg gcagtccgat
     2821 tgacgacaat gtcgccaata ttgtcattgc ccagctgctt ttcctggaaa atcaggacaa
     2881 agaaaaagac attaaaatct atattaactg ccccggcggc tcggttaccg ccggcctggc
     2941 tatttacgat accatgaaat atatcaagcc ggacgtgatg acgatctgcg tcggcctggc
     3001 ggccagcatg gccgctactt tgctgtcggc cggcgccaaa ggcaagcgat tatgcctgcc
     3061 taattctgaa attctgatcc accaggtaat gggcggggcc gaaggccagg cgactgacat
     3121 taaaatcagg gccgaaagaa ttttgaaaat caaaaccagg ctgaatgaaa ttctggccga
     3181 acacaccggc cagccgattg aaaaaattga aaacgacact gaccgcgact actttatgtc
     3241 agcccgggaa gccaaagact acgggattgt ggataagata attgactgaa aatataataa
     3301 ttaatcttaa attgaaagtt accagctgaa aagctggtga tttttgattt gaccagacat
     3361 gtggggaatc agaaggaatc agagggaatc agaggaaatc aggagggtat ttttacatct
     3421 tagtccctct caatccctct cattccttct tcccttgctt ccctttgctt cccttttgtc
     3481 ccccttatcc ccattacttg ctttttcccg ccttctgtga taatctctat ctaatcctaa
     3541 atataggatt aaaactacat ttttataatt aatttaattt tggccgggaa tttctaagca
     3601 aaagactaaa gctgttttga aagatctgaa agaaatttga ccctaatttt tcaacaaatg
     3661 tcttcttact cagtaaaaaa caggacaaaa atttgcttca gctcctttta aactaagcgg
     3721 ataaagcttg tcagcttttg atattgattt ccggccggct tagctaaaaa tatgtctact
     3781 atctatttaa taattattgt tgttggagtt cctctggcgg cagtttttgg gtatttaatc
     3841 agaaaaaata ttgccggccg gcaggttgat tcagccgaag ccaaagccca gaacatgctg
     3901 gtcgaggcta aaaacaaaca aaaagaactt ttactggaat caaaagacaa gtctctaaaa
     3961 ataattgagg acgctaaaaa agaagcccag gaaagacggc aggaaatcag ccatcttcag
     4021 agccgcctgg aaaaaaggga atctttattt gatcaaaaac tgcttgatct ggaaaacaag
     4081 aatcaaaaac tgaatgacaa ggccgcccag attgaagaag tcaaagaaca aatcca
//
LOCUS       DASWHZ010000097         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3515554, whole genome shotgun sequence.
ACCESSION   DASWHZ010000097 DASWHZ010000000
VERSION     DASWHZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3515554"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="murA"
                     /locus_tag="VGA49_02195"
     CDS             complement(<1..1016)
                     /gene="murA"
                     /locus_tag="VGA49_02195"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9664609.1"
                     /translation="MAKFVINGGKKLSGEIRVNGAKNNALKIIAASLLSDEEMIINNV
                     PDIEDVRRLLELLTDLGAVVKTDGNTVTISTKNVNSYQPNPKLVVKLRASTMLMGPLL
                     ARFGNVFMPFPGGCALGRRPIDLFLDGFKVLGADVEETEDGYWIKAEKLQGSKFVFPF
                     ITVTGTESLILTAVLTPGKTIIKNAACEPEILALAEYLNQQGAKITGAGTHTIEIEGV
                     EKLSAGTYNVIPDRIEAGSFAIMGVATDSPIKITNCNPDHLESLLVALHKVGGNFEIG
                     PDWIQLQPRDKTLRPYDMKTHEYPGFATDLQQPFTVLLTQTPGTSLIHETIFEGRFFY
                     IDML"
     gene            1251..2585
                     /locus_tag="VGA49_02200"
     CDS             1251..2585
                     /locus_tag="VGA49_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX9664610.1"
                     /translation="MVVAPILVIYTAGYRYNFKKNKLQKTGILVIESLPEKADVYINN
                     EYKKRTKAIIKNLFPDQYDVRVEKDGYYPWQKKLSVEAGQSAFATNIILFKKAEAEKI
                     IKEDIDFSSYSPDRKKLIYSVKNQSDKLLIVDLNGLKETIIFNNPQNKTENIEFKTNW
                     STSGNKILLTINKPDQGISQHWLIDINNPNNKKLLASPSRQNLEKISWEFNNEKILYG
                     LSAGTIYQIDVTNDEIKEFYSFDNIQDFQLYFDLIYFVTNDPASQKSYLKYVDLKNQE
                     TVYNIQNLPLSPNYQFLDHPGNKISLFDQNNNLFYLIKPGQDLITTQTEIFSNVNQIS
                     WSADFSKFLYFNDFELWIVYPKNDKSEKFLINRFSKKIKEAVWHPGFEYILFVLDNSI
                     QVIELDGRNGRNMNTLAELENITDLTIDFKSEKIYFKALEDDLNRAYFLEIN"
BASE COUNT          873 a          545 c          469 g          743 t
ORIGIN      
        1 ttaagcatat caatgtaaaa aaaacggcct tcaaaaattg tttcatgaat caggctggtc
       61 ccgggggtct gggtcagcaa aaccgtgaat ggctgctgta aatcagtcgc gaagcctggg
      121 tactcatgag ttttcatgtc ataaggccgc aaagttttat cccgcggctg aagttgaatc
      181 cagtcgggac cgatttcaaa attaccgcca actttatgta aagcgaccag caatgactcc
      241 agatggtcag gattgcaatt agtaattttg attgggctgt cagtggccac ccccataatg
      301 gcaaatgatc cggcttcaat cctgtcagga ataacattgt aagtaccggc ggacagtttt
      361 tcaacgcctt caatttcaat cgtgtgggtg ccggcgcccg taatcttggc gccctgctga
      421 tttaaatatt cagccagagc cagaatttcc ggttcacagg cggcattttt aataattgtt
      481 ttgcccggag ttaaaaccgc cgttaaaatt aaactttccg ttccggtcac tgtaataaaa
      541 ggaaagacga actttgatcc ctgtaacttt tccgctttga tccagtaacc gtcctctgtt
      601 tcctcaacat cagctcccaa aactttaaag ccgtccaaaa acagatcaat cggccgccgg
      661 cccagggcgc aaccgcccgg aaaaggcata aaaacgtttc caaacctggc taaaagcggg
      721 cccatcagca ttgttgaagc ccgaagttta acgaccagtt tggggttagg ctggtaacta
      781 ttgacatttt tagtggaaat cgttactgta ttaccgtctg ttttaacgac cgccccaagg
      841 tcagttaaaa gttccaaaag acgccgaaca tcttcaatat ccggcacatt attaataatc
      901 atttcctcgt cagacaacag actggcggcg ataatcttta aagcgttgtt tttagcgcca
      961 tttacccgaa tttcgcctga taatttcttg ccgccattga taacaaattt agccatagaa
     1021 taaaattatt ttataaccag taaactatta aatagtaata tatttatccg aaatattcaa
     1081 gtgaagtgat ataataacca agggagatca gggttaatga aggttaatga aggtagatca
     1141 gggggattcc ctaatctacc ctaacatacc ttaatattcc ttcataaacc ctacttgtta
     1201 tgcaaaaaaa atacagacat gtaatcagct catcattact aatattgttt atggttgttg
     1261 cccctattct ggtgatctat actgccggct atcgttataa tttcaaaaaa aacaaactcc
     1321 agaaaaccgg cattctggtg attgagtctt tgcctgaaaa ggccgatgtc tatattaata
     1381 atgaatacaa aaaaagaacc aaggccatta ttaaaaatct ttttccggat cagtatgacg
     1441 ttcgggtgga aaaagacggc tattatccct ggcagaaaaa actgtcagtt gaggctggcc
     1501 agtcagcttt cgcgactaat ataattttat ttaaaaaagc tgaagctgaa aaaatcatta
     1561 aagaagacat tgattttagc tcctatagtc cggacagaaa aaaattaatt tacagcgtca
     1621 agaatcaatc agataaactt ttaatcgttg atctgaacgg cttaaaagag acgataattt
     1681 ttaataaccc ccaaaacaaa actgaaaata ttgaatttaa aacaaactgg tcaacttccg
     1741 gcaataaaat attgctgaca attaataagc ctgaccaggg aatcagtcag cactggctga
     1801 ttgatataaa taatcctaat aataaaaaat tactggccag cccgtccaga caaaaccttg
     1861 aaaaaatcag ctgggaattt aataatgaaa aaattctcta cggtctgtcg gccggaacca
     1921 tctatcagat tgatgttact aatgacgaaa tcaaagaatt ttattcattt gataatattc
     1981 aggattttca gctttatttt gatctgattt attttgtaac taatgacccc gccagccaaa
     2041 aaagctatct aaaatacgtt gatttaaaaa accaagagac tgtctacaac atacaaaact
     2101 tacccctatc accaaattac caatttctgg atcatccggg aaacaaaatt tctctctttg
     2161 accaaaacaa taatcttttc tatctaatca agcccggcca ggacttaatc accacccaga
     2221 ctgaaatatt cagcaatgtt aatcaaatca gctggtcagc cgatttttca aaatttttat
     2281 attttaatga ctttgaactg tggatcgttt atcccaaaaa cgataaaagc gaaaaatttc
     2341 taatcaaccg cttcagtaaa aaaataaagg aagccgtctg gcatcctgga tttgaatata
     2401 ttttatttgt tttggataac tcaattcagg tcattgaact tgacggccgc aacggccgca
     2461 acatgaacac cctggctgaa cttgaaaaca tcactgattt aacaatagat ttcaaaagtg
     2521 aaaaaatcta ttttaaagcg ttagaagatg atctcaaccg ggcttatttt ttggaaatca
     2581 attaattatc ccactgggcc actaaattgg acgctgtcag caggctgtca
//
LOCUS       DASWHZ010000098         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2444048, whole genome shotgun sequence.
ACCESSION   DASWHZ010000098 DASWHZ010000000
VERSION     DASWHZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444048"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VGA49_02205"
     CDS             <1..743
                     /locus_tag="VGA49_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX9664611.1"
                     /translation="GQLYLEAAIMSFGRCFDFGPVFRAEKSKTRRHLTEFWMMDAEAA
                     YVEHQDNMKIQEELILFILQKVLDKHQKDLEILERDPEPLKNIKAPFIRKTHAEVIQE
                     LQAMGSDIKENNDLGADDETNLTQKYDQPIFVERYPAAVKAFYMKRDPENPDLVLNND
                     LLAPEGYGEIIGGSQREDDYQKLLQRIKEHNLSVEDLNWYLDLRKYGSVPHSGFGYGL
                     ERIVGWVCGLHHVRETIPFPRTIYRLKP"
     gene            990..2399
                     /gene="aspS"
                     /locus_tag="VGA49_02210"
     CDS             990..2399
                     /gene="aspS"
                     /locus_tag="VGA49_02210"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9664612.1"
                     /translation="MQRTVNKDTINAVGNNVLVKGWVHSIRKMGRIVFIDLRDFSELL
                     QIVFVLDDLDKKSQKFLEELHSEYVIQVEGIIQKRNEKQINQKILTGAVELLAGKLEI
                     IAKSEIPPFEIDKDTININEEVRLKHRYLDLRSLRLHSNFILRHNIVKHIRKFFYDKD
                     FVEIETPFISKSTPEGARDFLVPSRLHAGKFYALPQSPQQYKQLLMIGGFEKYFQIVR
                     CFRDEDFRGNRQPEFTQLDLEMSFVDQEDILSLIEELMTGLIEKIVPYKKISYTPFPR
                     LTYREVMKKYKTDTPDLRKEKGDKDELAFCWVTDFPMFEYKEGDKRWGAVHHPFTMPY
                     KEDAHLLKTPEKLGEVRAHQYDLVLNGQEIFGGSIRNHDPKFLGQVFKALGHKKKEIE
                     QKFGHLLEAFKYGAPPHGGIAGGLDRLVMVLANEENIREVIAFPKTGEGYDPMMDSPG
                     EVSKQQLEELNIRVKRKQK"
     gene            2406..>2524
                     /locus_tag="VGA49_02215"
     CDS             2406..>2524
                     /locus_tag="VGA49_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX9664613.1"
                     /translation="MTKVKFKLEQFEGPLDLLLQLIEDKELDISQVSLGAVAD"
BASE COUNT          781 a          446 c          570 g          727 t
ORIGIN      
        1 ccggccagct ttatctggaa gcggctatta tgtcttttgg ccggtgtttt gattttggcc
       61 ctgttttccg ggcggagaaa tccaagaccc gaaggcactt aacggaattc tggatgatgg
      121 acgctgaagc cgcctatgtt gagcaccagg acaatatgaa aattcaggaa gagttaattt
      181 tgtttatttt acaaaaagtt ttagacaaac atcaaaaaga tctggaaatt ttggaacgtg
      241 atcccgaacc tttaaaaaat atcaaagcgc cttttattcg caagacccac gctgaagtga
      301 ttcaggagct tcaggccatg gggtcagata ttaaagaaaa taatgatttg ggcgctgatg
      361 atgaaactaa tctaacgcaa aaatatgacc agccgatttt tgttgaaaga tacccggcgg
      421 ctgttaaagc tttttacatg aaaagagacc cggaaaatcc tgatttggtt ttaaacaatg
      481 atttgctggc gccggaaggc tatggcgaaa tcatcggcgg ttcccagcgt gaagatgatt
      541 atcaaaaatt attacaaaga atcaaagaac ataatttatc agttgaagat ctgaattggt
      601 atctggattt gagaaaatac ggttcagtcc cgcattccgg ctttggctat ggtttggaaa
      661 gaattgtcgg ctgggtctgc ggcctgcatc atgtccggga aactattccg tttccacgga
      721 ctatttatcg tttaaagccg taaaatatat cattttcgcg gtatcaaaca gcgccaggct
      781 gttttttgtt ttttggttga aatatggtat aatttattaa atttcaaatt acaaagctca
      841 aatgtcaatt gaaatccaaa gctcaaatcc cgtcagtcac gactgacggg atcaaatgtc
      901 aaataataaa atttttggat tttagatttt ggatttgatt tggaacaatt tggattttga
      961 catttaacat tttaattata aatgaattta tgcaaagaac tgttaataaa gacactatta
     1021 atgcggtcgg caataatgtt ttagtcaagg gctgggttca ttcaattaga aagatgggca
     1081 ggattgtttt tattgatttg agggattttt ctgaactcct ccagatagtt tttgttctgg
     1141 atgaccttga taagaagtct caaaaatttt tggaggagct ccatagtgaa tacgttattc
     1201 aggtggaagg gattattcaa aaaagaaatg aaaagcagat taatcaaaag attttaaccg
     1261 gcgcggtgga gctgctggcc ggcaaactgg aaattatcgc caagtcagaa attccgccgt
     1321 ttgaaattga caaagacaca attaacatta atgaggaggt ccgtttgaag caccgttatc
     1381 ttgatttacg gtctttgcgg ctgcattcta attttatcct gcggcacaat attgttaagc
     1441 atatccggaa atttttttat gataaagatt ttgtggaaat tgaaaccccg tttatttcca
     1501 aatcaacgcc tgaaggcgcc cgggattttc tggttccgtc ccggctccac gccggcaaat
     1561 tctacgctct gccccagagc ccccagcaat acaagcagct gttaatgatt ggcggttttg
     1621 aaaaatattt tcaaattgtc cgctgtttcc gcgatgaaga tttccggggt aaccgccagc
     1681 ccgaatttac tcagcttgat ctggaaatga gttttgtgga ccaggaagac attttgagct
     1741 taattgaaga actgatgacc ggtctgatag agaaaatcgt tccttataaa aaaatcagct
     1801 atactccctt tccccggttg acataccggg aagtcatgaa aaaatataaa actgacacgc
     1861 ctgatttgag gaaagaaaaa ggagataaag atgaactggc tttttgctgg gtaactgatt
     1921 ttccaatgtt tgaatataaa gaaggcgata agcgctgggg agcggttcat catccattta
     1981 caatgccata taaagaagat gctcatttac ttaaaactcc cgaaaagctg ggagaggtca
     2041 gggcccatca atatgatctc gtcctgaacg gtcaggaaat ttttggcggc agtatcagaa
     2101 accatgatcc aaaattttta ggacaggttt ttaaagctct gggtcataag aaaaaagaaa
     2161 ttgagcaaaa attcggacat ttgctggaag cttttaaata cggcgcgccg ccccacggcg
     2221 ggatcgccgg cggtttggac aggttagtaa tggttttggc caatgaggaa aatatcaggg
     2281 aagtgatcgc ttttcccaag actggcgaag gctatgatcc aatgatggat tcaccgggag
     2341 aagttagcaa acagcaattg gaggaactta atattagagt caaaagaaag caaaaataat
     2401 gactaatgac aaaggttaaa tttaaattag aacagtttga aggcccgctg gatttactgc
     2461 ttcagttaat tgaagataaa gagcttgata tttcccaggt ttctttggga gcggtcgctg
     2521 atca
//
LOCUS       DASWHZ010000099         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_200622, whole genome shotgun sequence.
ACCESSION   DASWHZ010000099 DASWHZ010000000
VERSION     DASWHZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200622"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VGA49_02220"
     CDS             <1..533
                     /locus_tag="VGA49_02220"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664614.1"
                     /translation="LFVIEAHQTPTTQDKHLAGEIIESDSSIIVVANKWDLVSIKNKD
                     LKKQNLLFQSIFPFLTWAPILYVSAKTKKNIPKIMDLALEIKEERQKKITDRILDRFF
                     KKLIAYHRPKKAKGTKIPYLYSLKQVRTNPPVFQLEIGEKGVLSKAYFKYLENVLRKK
                     FGFIGAPVKVILKKRK"
     gene            643..1218
                     /gene="pth"
                     /locus_tag="VGA49_02225"
     CDS             643..1218
                     /gene="pth"
                     /locus_tag="VGA49_02225"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9664615.1"
                     /translation="MKLIVGLGNPGKKYEGTRHNVGFVVIDQLADQNFKLNKKSNSLI
                     LKTKIDSKDVILAKPQTFMNSSGQAVAALAGYYKVKPENIIVIHDEIDLPLGTIRISQ
                     DSSAAGHNGVQSIINSLGTRNFIRIRIGIDPDSKTIATAEPRDLKLNNFVLNKFTAKE
                     EKIVKKVIDQAVKALQTIITENVTVAMNKHN"
BASE COUNT          553 a          346 c          302 g          448 t
ORIGIN      
        1 ctttgttcgt gattgaagcc catcagacgc cgaccaccca ggataagcat ctggccggag
       61 aaattattga atctgattcg tcaatcatcg tggtggccaa taaatgggat ctggtttcga
      121 tcaaaaataa agatttaaaa aaacaaaatc tcctgtttca gtcaattttc ccctttttaa
      181 cctgggcgcc aattttatat gtcagtgcta aaaccaaaaa aaatatccca aaaattatgg
      241 atctggctct ggaaatcaaa gaagagcgtc aaaaaaaaat caccgaccgg attttagaca
      301 gattctttaa aaaactgatt gcttatcacc gccccaaaaa agccaaaggc accaaaatcc
      361 cctatcttta tagtttaaaa caagtcagaa ccaatccgcc ggtttttcaa ctggaaatag
      421 gagaaaaagg agttttatcc aaagcttatt tcaaatatct ggaaaacgtc ttgagaaaaa
      481 aattcggctt tattggcgcg ccagttaaag taattttgaa aaaaagaaaa taattaaata
      541 accagtcact agtcaacggt caacagccac tagggattta ctgataactg aaccctaatc
      601 ccaaccctga tgactgttgg ctaatgactg ttggctacat tgatgaaatt aatagtcggg
      661 cttggaaatc caggaaaaaa atatgaagga accagacata atgtcggctt tgttgtcatt
      721 gatcagttag ccgaccaaaa ttttaagtta aataaaaaat caaattcctt aattttgaaa
      781 acaaaaattg attctaaaga tgttatcctg gctaaaccgc aaacttttat gaacagttcc
      841 ggtcaggcgg tcgccgccct ggccggttat tataaagtaa agcctgaaaa tataattgtt
      901 attcatgatg aaattgacct gcctttggga acgatcagaa tttcccagga ctcatcagcc
      961 gccggccaca acggagttca gtcaatcatc aattccctgg gaaccagaaa ttttatcagg
     1021 atccgaattg gcattgatcc cgactcaaaa acaatcgcca cggcagaacc ccgggattta
     1081 aaactgaata attttgtctt aaataaattc accgccaaag aagaaaaaat tgttaaaaaa
     1141 gtaattgatc aggcggttaa agcattacaa actatcatca ccgaaaacgt tactgtggca
     1201 atgaataaac acaactaacc cccttcatcc accctcatca actcccgatc ccgcctatgg
     1261 cgggatcgag gatccccgat cccgccatag gcgggatcgg gacttcatct accctaataa
     1321 aaaaacccaa ttactaccta ccagcgtaag gccctcaaga aatcttgaat gccttaactt
     1381 atgaataccg gcttatatga gaccaggagg ggggtctcaa gctttcttcg ctggtaagta
     1441 gaaattgagc tattttaact aaatcattgt atcacaactg aactgttttg tcaagaatag
     1501 ttgacttttt gtcaacagga agttaaaatg tcatagtttc aagtatttta aaatgtcata
     1561 ccaggttctt tttgcttcac tgttgtcttg aggcaacctc tttgtttcca tgggtggttg
     1621 gcggcaggct tatgagcctt tgattgatt
//
LOCUS       DASWHZ010000100         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_585109, whole genome shotgun sequence.
ACCESSION   DASWHZ010000100 DASWHZ010000000
VERSION     DASWHZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585109"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VGA49_02230"
     CDS             <1..530
                     /locus_tag="VGA49_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9664616.1"
                     /translation="QYVIGTVCRLKPESGLGYLLKSLKTASEIIPNIHLIIIGEGGEK
                     QKLIWLARQLGIEQKVRFVGWQDDLVRWINNFDLYILPSLVKDSVSTVLLEVMALGKP
                     IIATKTGANPEIVEHGQTGIVIEPENPEMMAQVIINLYNNPDWLEQYGINGRKRIEEY
                     FDLEKVVEEYNRILT"
     gene            730..1377
                     /locus_tag="VGA49_02235"
     CDS             730..1377
                     /locus_tag="VGA49_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664617.1"
                     /translation="MRNIIISLLLLVATGCQSLLGTASSTSIEDGKLSYGDLGGDPQR
                     AAEAQARINESQVDRGIAAVYVAAASTAQERQALRKQVVEAYLAESDPAKLEKLLQLL
                     QTMEELEADSTPVLPPTSPGLVGNYRMVIVNDSKFDLSVTLSGVGGAQTIQLRSGQHR
                     VVKVAGGEFEIVARRSADGRLFWRNRGVINDTRGDATIPSLPGRRYDAAIRLSDQ"
     gene            complement(1492..1950)
                     /locus_tag="VGA49_02240"
     CDS             complement(1492..1950)
                     /locus_tag="VGA49_02240"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX9664618.1"
                     /translation="MEKIKNWWKIKFLSKPVAVLTIVSMLLFGVSLIMPASAQGLDLT
                     TELKKAGEPAFGTGQKPLAETVGDIIQVFLGFLGTIALILVLYGGFMWMTAGGNEEKV
                     TKAKTLLRNAVIGLIIVISAYSITYFVITQIGQATGVGVGAGTGTGQIQQ"
     gene            complement(2099..2506)
                     /locus_tag="VGA49_02245"
     CDS             complement(2099..2506)
                     /locus_tag="VGA49_02245"
                     /inference="COORDINATES: protein motif:HMM:NF039493.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664619.1"
                     /translation="MINNFQKIKFLSIVSLTVLFILAAPLQIEAQFGETGLETLDSTG
                     WGAGFDITKEAGLSVTVGNIIKTILGFLGITAVIIVLYGGFMWMTAGGNEEKVTKAKT
                     LLRNAAIGFIIIAASYAITFFVVERLQQAALGG"
BASE COUNT          691 a          659 c          580 g          659 t
ORIGIN      
        1 tccagtatgt cattgggacg gtctgccgcc taaagccgga atcggggtta ggctatcttt
       61 tgaaatcttt aaaaaccgct tcagaaatta ttcccaatat ccatttaatc ataattggcg
      121 agggcgggga aaaacaaaaa cttatctggc tggccagaca gctggggatt gagcaaaaag
      181 tccgctttgt cggctggcag gacgatctgg tccgctggat caataatttt gatctgtata
      241 ttctgccgtc gctggtcaaa gactcggtca gcacggttct gctggaagtg atggctttgg
      301 gcaagccgat cattgccacc aagaccgggg ctaatcccga aatcgttgag cacggccaaa
      361 ccggcatagt aattgagccg gaaaatccgg aaatgatggc tcaggtgatc atcaaccttt
      421 acaacaatcc cgactggctg gagcagtatg ggataaatgg gaggaaaagg attgaggagt
      481 attttgattt agagaaagtt gtagaggaat ataatcggat attaacctga taacagggaa
      541 gcaaagggaa tcaaggggag gcaaagggaa aaaaaccccc tttttccttt tgcttcaact
      601 tgcttcctta ttccctcgct aatcttagcc ccaaaaaacc atttttcctt gacaaaaatt
      661 ccaaaatatg ctatttttat ttgataacct gtcctttgaa acacgtgatc ttttggaaag
      721 gggcttaaca tgcgtaacat catcatctcc cttcttcttc tcgtcgccac cggctgccag
      781 tcccttctcg ggactgcctc ctcgacttcc atcgaggacg gcaagctgtc ctacggggat
      841 ctgggcggag atcctcaacg ggcggccgag gcccaggcgc ggataaacga gagccaggtg
      901 gaccggggga tcgcggccgt ctacgtggcc gccgcctcca ccgcccagga gcgccaggcg
      961 ctccgcaagc aggtcgtcga ggcctacttg gccgagtccg acccggccaa gctggaaaag
     1021 ttgctccagc ttctgcaaac gatggaggag cttgaggcgg acagcacgcc ggttttgcct
     1081 ccgaccagcc ccggcctcgt tggcaattac cgtatggtaa ttgtcaacga ctcaaagttt
     1141 gacctatcgg tcactttgag cggggtcggc ggtgcccaga ccatccagct ccgctccggc
     1201 cagcaccggg tggttaaggt ggctggcggg gagttcgaga ttgtcgctag acgatccgcc
     1261 gatggccggt tgttttggcg aaaccggggt gtcataaatg acacccgggg ggacgcgaca
     1321 atcccctccc tccctggtcg gaggtatgac gccgccatca gactctccga ccaataagcc
     1381 gttcggctca cctccgaacg gaaaaatcac aaagccgttc ggctcaccca ccgaacggct
     1441 tttttcttgc cctccgagtc cccggcaaag gaggtttttt tattttttta tctattgttg
     1501 tatttgacct gtccctgttc ccgcgcccac gcccacgcca gtcgcctggc caatctgggt
     1561 gatcacgaaa taggtaattg aataagcgga aatgacaatg atcagaccaa tcaccgcgtt
     1621 ccgcaacaat gtcttggctt tggtaacttt ttcctcattg ccgccggccg tcatccacat
     1681 aaatccgccg tataaaacca aaatcaaagc aatcgtcccc aaaaatccca aaaaaacctg
     1741 aatgatatcg ccgactgttt cagccagcgg tttttggccg gttccaaaag ccggctcacc
     1801 ggcctttttc agttcggtcg ttaaatcaag tccctgggct gaagctggca ttattaaact
     1861 aacaccaaac aacagcattg agacaatcgt taagacagcg accggctttg ataaaaattt
     1921 tattttccac caatttttta ttttttccat atgttttata tattatacca atactcgatt
     1981 ctaatataca aatttgtact aattatacta ataacctcct gataaattcg tgtctttagt
     2041 atcaattagt atattggcac cgatgattgg tatttgttct aaatttgata atctaaactc
     2101 aaccgcccag ggctgcttgc tgcagcctct caaccacaaa aaaagtaatg gcgtaagagg
     2161 cggcgatgat gataaaacca atggcggcgt ttctcaacaa tgttttggcc ttagtgacct
     2221 tttcctcatt gccgccggcc gtcatccaca taaaaccgcc gtatagaact ataatcacgg
     2281 ccgttatccc taaaaatcct aaaatggtct taatgatatt accaactgtc acgctcaaac
     2341 cggcctcctt agtaatgtca aaaccagctc cccaaccagt agaatctaat gtctctaatc
     2401 ctgtctcacc aaactgggct tcaatctgca aaggtgccgc cagaataaat aaaactgtca
     2461 ggctgacgat gcttaaaaat tttatttttt gaaaattatt gatcattttt attttatgct
     2521 aggccaaaaa taatattcgg ataaaaataa accgaacaat cgcgtaagcc ataactatga
     2581 taattaaac
//
LOCUS       DASWHZ010000101         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3521047, whole genome shotgun sequence.
ACCESSION   DASWHZ010000101 DASWHZ010000000
VERSION     DASWHZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521047"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..293
                     /locus_tag="VGA49_02250"
     CDS             12..293
                     /locus_tag="VGA49_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9664620.1"
                     /translation="MAKMTKSQMYQELSTKTGMSKKEVTECMDALVNLALDQVKSAGE
                     FTIPGIGKLVKQHRKARMGRNPATGEQIQIPAKTVVKFRVAKQAKESVL"
     gene            complement(347..1285)
                     /gene="fmt"
                     /locus_tag="VGA49_02255"
     CDS             complement(347..1285)
                     /gene="fmt"
                     /locus_tag="VGA49_02255"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9664621.1"
                     /translation="MKQNKKIIFIGTSEFASIILENLIKNDQAPHIIVSRPDKPRGRK
                     KEIIDNQVVKFAKEKKTQLLQPEKISSIFNLLKETDPDLIIVAAYGRIIPKKIIDLPR
                     FGSINVHPSLLPKYRGPSPIQFTLLNGDQFTGASIMLMDDKMDHGPIIAQEKHKISNR
                     ETFLTLQKELADLSSKLLIKTLPQWLNQSIKPMEQNHQFATFTKILTKKDGKIDWSKT
                     ADDIDQQIRAFDSWPGTWAGWNNKILKIHKAHPAEIDLNNQQFGQVFQDDYNNIIVRC
                     GFGNLILDRLQMEGKKEMSAADFVRGHQNFIGSVLN"
     gene            complement(1272..1742)
                     /gene="def"
                     /locus_tag="VGA49_02260"
     CDS             complement(1272..1742)
                     /gene="def"
                     /locus_tag="VGA49_02260"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9664622.1"
                     /translation="MEIVRYPNKILRAKSKEISDFESPDIKKLIAEMIPTMKNANGIG
                     LAAPQIGKNIRLTVIETKNGPLPLINPKIIKKSFKKEISEEGCLSLPGVFGTVKRHKK
                     IKFKALTPEGQEMIYDAEGLFARVVQHEIDHLDGILFIDKAIEITEGKIKDEAK"
     gene            complement(1753..3669)
                     /gene="priA"
                     /locus_tag="VGA49_02265"
     CDS             complement(1753..3669)
                     /gene="priA"
                     /locus_tag="VGA49_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9664623.1"
                     /translation="MFAEIIPIKRFPRKIFFFDYLISDDLKDQIRPGHLAEIPFKNRL
                     LAGLVINIKSQSETPEATIKQVKSLLAADQILYQPQTDLILDLADYYNISPGHILKNI
                     LPDIPKRRLARIPPALTVQTQAEELSVKKDSLSLIKSNFREIETTADKPFLLHYQSKN
                     DKETVIFKTAERILKDGGQIIYLVPRISLINSLAALYKKLGPVAVLHGQLAKSTYWDQ
                     WQKISQNQAKIIIGTRTALFAPVKSLKLIMVDSEESSDYKQSEQNPRYDARLAAEKLS
                     GLTKSKLIYCSQSPQIISYYKSQQNKYKLLNLSAITPKKTEIVDLNMEIKAGNYGSVS
                     YKLQTCLENALQDNKKVLLYLDQRGYSPVFACLDCSYIFKCPDCRLPFACHLNKLNCH
                     RCFKEEPILSACPECRGANLKTIGYGTRRLEEEVKKLFSKAKILTVDKDVKTVDLKQS
                     YQILIGTQYLLQNYPQLLLYLGLVAVVTADNLFYIPDFMINETIFQKINSLINLPNSN
                     AKFIIQTLNPENFVIQAAAQNNYQKFFNQEIKNRKEFNYPPFVRLIKLTYKNQNIKKA
                     DFEANNLYQHLKNKSKNLSIEILPPISGLKQHNKFIRNIIIKLPLNLRSKTSEILDPV
                     YSDWIIDIDPVSLL"
     gene            complement(3673..4572)
                     /locus_tag="VGA49_02270"
     CDS             complement(3673..4572)
                     /locus_tag="VGA49_02270"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9664624.1"
                     /translation="MYLFSLHPQKQWAQKLAKKSGLKSGRLNIKQFTDQEYYIQVLEK
                     VTNQEVFVLSQIGPPADNLVKTLILLNALKSNGAKKIFVIIPFFSYSKQDRVDQPGAP
                     VSAKLMADLLKTAGADKIISADIHSPLTRKYIGRRLVEIQTMPLLAQYFKNKKISDLT
                     ILSLDTGSIKRAKQFARLLKTPSVARAKKHRPKPEVAKILKIKGNLEGKNVIIVDDFI
                     ETGGTMTAVVEMLKKIGVKNIFAAVSHGILAGPAVKNIKRSPIKKLVLTDSIPLPPEK
                     RLDKIEVLSVIDLISQQVNNLKK"
     gene            complement(4576..5214)
                     /locus_tag="VGA49_02275"
     CDS             complement(4576..5214)
                     /locus_tag="VGA49_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX9664625.1"
                     /translation="MYFKDRPDAGKKLADQLKKYKKNPETVVIALPRGGLVTGYEVAK
                     KLKLPLDIIVSKKIGAPGDPELAIGSVNMDGQVLIDQDQLNLFNISQDYIDQQAIILK
                     KEIKQKLIKLRGTGQIPSYRHKTVIVVDDGVATGYTMKAAINYLKKQPVKKIVLAVPV
                     GPSGTIEALKKEVDDLICLSTPAMFMAVGAFYENFEQITDEECRQLLKKAKK"
     gene            complement(5315..6100)
                     /gene="amrB"
                     /locus_tag="VGA49_02280"
     CDS             complement(5315..6100)
                     /gene="amrB"
                     /locus_tag="VGA49_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX9664626.1"
                     /translation="MLCFSAIVPHSPLLIPAIGQENIEKFKTTIKALDDLKKKFEEAK
                     PDTVVVISPHGPLLADAFTINLNSDYLGSFEEFGEFATKLEFKPDQQLLSQIRERSTK
                     ELPIVLTSNQKLDYGTAVPLFYLTANQPILPIVPVGYSNLNFQKHYQFGQIMSEEVSK
                     IGRRRVAVIASSDLSHSLTEDAPGGYNLKGKEFDQKLLTLLKDNTFEEITKIDPELSK
                     DAAECGLRSIIILLGVLTEKKPKFEEISYEAPFGVGHLVGQFT"
BASE COUNT         1764 a         1157 c         1245 g         2044 t
ORIGIN      
        1 aaaggaaaag tatggccaaa atgaccaagt ctcaaatgta tcaggagctc tccaccaaga
       61 ctggcatgag caaaaaagag gtgactgaat gcatggacgc tttagttaac ctcgccctgg
      121 atcaggtaaa atcagccggt gaatttacaa taccgggaat tgggaaatta gttaaacaac
      181 acagaaaagc cagaatgggc cgtaatccgg ctactggcga gcaaattcag atcccggcca
      241 aaactgtcgt taaattcaga gtcgccaaac aggccaaaga gtcagttctt taattaaaat
      301 tttcacaaaa aaacaagtct ttggcaggct tgtttttttg tcgggtttaa ttcaaaactg
      361 accctatgaa attttgatga cctctgacaa aatcagccgc tgacatttct tttttgcctt
      421 ccatttgaag ccggtctaaa attaaatttc caaagccgca ccggacaata atattgttat
      481 aatcatcttg gaaaacttgg ccaaactgtt gattgtttaa atctatttca gcgggatgag
      541 ctttatggat ttttaggatt ttgttattcc agccagccca ggttcccggc caggaatcaa
      601 aggctctgat ttgctgatca atatcatccg cggtttttga ccagtcaatt ttaccatcct
      661 ttttagttaa tatttttgta aaagtcgcga attgatgatt ttgctccatt ggtttaatgc
      721 tttgattaag ccattgcggc agagttttaa ttaaaagttt agaacttaag tcggccagtt
      781 ctttttgaag ggttaaaaag gtttccctgt tagaaatttt atgtttttcc tgagcgataa
      841 ttggcccgtg atccatttta tcgtccatta gcataattga cgcgccggta aactgatcgc
      901 cgtttaatag ggtaaactga atcggtgaag gcccccgata tttaggaagg agagaggggt
      961 ggacgttaat tgatccaaaa cggggcaggt caataatttt tttgggaatt atccggccat
     1021 aagcagccac aataattaaa tccgggtcgg tttcttttaa gagattgaaa attgatgata
     1081 ttttttcagg ctgaaggagt tgtgtttttt tttctttggc aaatttaaca acctgattat
     1141 ctataatttc cttttttcgt ccccgcggct tatcaggccg ggagactatt atatgagggg
     1201 cctggtcatt tttaataaga ttttccagga ttattgaggc aaattcagaa gtgccgataa
     1261 aaataatttt tttattttgc ttcatctttt atttttccct cggttatttc aatcgcttta
     1321 tcaataaata aaataccgtc caaatgatca atttcatgtt gcacgacccg ggcgaacaaa
     1381 ccttcggcgt cataaatcat ttcctggcct tcaggagtta aagctttaaa tttaattttt
     1441 ttgtgacgct tgactgtccc aaaaacgccg ggaaggctta aacagccttc ctcggaaatt
     1501 tcttttttaa atgatttttt tattatttta ggattaatca aaggcagggg accgtttttg
     1561 gtttcaatca ctgttaacct gatattttta ccaatttggg gggcggccag accaatgccg
     1621 ttggcatttt tcattgtggg aatcatttca gcgattagtt ttttaatatc aggactctca
     1681 aaatcactaa tttcctttga cttggccctc aaaattttat tgggatacct gactatttcc
     1741 ataatatttt ttttaaagta atgaaacggg atcaatgtct attatccagt ctgaataaac
     1801 cggatcaagg atttcagatg ttttactcct taaattaagg ggtaatttaa taataatatt
     1861 tctaataaat ttattatgct gtttcagccc ggaaattggc ggtaaaattt ctattgataa
     1921 attttttgat ttgtttttta aatgctgata gaggttatta gcttcaaagt cagctttttt
     1981 tatattttga tttttatagg tcagtttgat taatctgaca aaaggagggt aattaaattc
     2041 ttttctgttt ttaatttcct ggttaaaaaa tttttgataa ttattttgag ccgccgcctg
     2101 gattacaaag ttctcaggat taagggtctg aattataaac ttcgcattgg aatttggtaa
     2161 attaatcagg ctgttgattt tttgaaagat tgtttcatta atcatgaaat ccgggatata
     2221 aaacagatta tcagccgtta ccacggccac cagcccgaga tataacagta actggggata
     2281 gttttgaagc aaatactggg tgccaattaa aatttgataa ctttgtttta aatctactgt
     2341 tttaacatcc ttgtcaactg tcaaaatttt tgctttggaa aataattttt taacttcctc
     2401 ttccagccgt ctggtgccat aaccaatagt tttcagatta gcgccccggc attcaggaca
     2461 ggctgagaga attggctctt ctttaaaaca acgatgacag tttaatttgt tcaggtgaca
     2521 ggcaaaaggc agccggcagt caggacattt aaaaatataa gagcaatcca gacaggcaaa
     2581 aacaggggaa tagccgcgct gatcaagata taataacact tttttattgt cttgtaaagc
     2641 gttttccagg caggtctgca atttatagct gacagaacca taattgccag ctttgatttc
     2701 catattaagg tcaacgattt cagttttttt aggagttata gcagacaagt taagcagttt
     2761 atatttattt tgctggcttt tataatagct gataatttgg ggagattgac tgcagtaaat
     2821 taattttgat ttagtcaggc cggatagttt ttcagccgcc agtctggcgt catatcgggg
     2881 attttgttcc gactgcttat aatcactgct ttcctctgaa tctaccatga ttaattttaa
     2941 agacttaacc ggcgcgaaaa gagctgttct ggtcccaata attattttag cctgattttg
     3001 gctgattttt tgccactgat cccaataagt tgatttggcc agctgaccgt gaaggactgc
     3061 caccgggccc agctttttat aaagggccgc caaagaatta atcagagaaa ttcgtggaac
     3121 cagataaata atctgtccgc cgtcctttaa aattctttca gctgttttaa aaataacggt
     3181 ctctttatca tttttagact gataatgaag taaaaaaggc ttgtccgcgg tcgtctcaat
     3241 ttcgcgaaaa ttacttttta tcaaagataa agaatctttt ttaactgaca gctcttcagc
     3301 ctgggtttgg acggtcaaag ccgggggaat cctggccagt cttcttttag gaatgtcagg
     3361 caggatgttt tttaaaatat gcccgggaga aatattgtag taatcagcca gatcaagaat
     3421 cagatcagtt tggggctggt ataagatttg atcagcggct aataagcttt taacttgttt
     3481 gatagtagcc tccggagttt cagactgact ttttatgtta ataaccagtc cggctaataa
     3541 tctgttttta aaggggattt ccgctaaatg cccgggccta atctgatctt ttaaatcatc
     3601 agaaattaga taatcaaaaa aaaagatttt tcgggggaat cttttaatcg gaataatttc
     3661 cgcgaacata gtttattttt ttaaattatt tacctgctgg ctaatcaggt caatcacgga
     3721 cagaacttca attttatcca gtcgcttttc tggcggtagg ggaatgctat cggttaaaac
     3781 caatttttta atcggtgatc ttttgatgtt ttttacggcc ggcccggcta aaattccatg
     3841 actgaccgca gcgaaaatat ttttaacgcc gattttttta agcatttcaa ccacggccgt
     3901 cattgttccg ccggtttcaa taaagtcatc aacaataata acgtttttgc cttccaggtt
     3961 gccttttatc ttaagaattt tggcgacctc gggcttcggc cggtgttttt tggctctggc
     4021 gacagacggt gttttcagca atctggcaaa ttgtttggcg cgtttaattg atccggtgtc
     4081 aagggacaaa attgtcagat cagaaatttt tttattttta aaatattgag ccagcagagg
     4141 catcgtctga atttcaacca gccgccggcc aatatatttt cgcgttaaag ggctgtgaat
     4201 atcagctgaa ataattttat cagctccggc agttttaagc agatcagcca ttaatttggc
     4261 gctgaccggc gcgccaggct ggtcaactcg gtcctgcttg gaatagctga aaaagggaat
     4321 aatgacgaat atttttttgg cgccattact ttttaaagca ttcagcaaaa ttaaagtttt
     4381 taccaaatta tcggctggcg gaccaatttg ggacaggaca aaaacttctt gattagtaac
     4441 tttttccagc acctgaatat aatattcctg atccgtaaac tgtttgatat ttaaccgtcc
     4501 tgattttaag cccgattttt tggctaattt ttgagcccat tgtttttgag gatgaagaga
     4561 aaataaatac atattttatt ttttggcctt ttttaaaagt tgccggcatt cttcgtccgt
     4621 gatttgttca aaattttcgt aaaaagcgcc gactgccata aacatcgctg gcgttgacag
     4681 acagattaaa tcatcgactt cttttttgag agcttcaatt gtccctgatg gaccaaccgg
     4741 cacggccagc acgatttttt taaccggttg tttttttaaa taattaatag cggctttcat
     4801 ggtatagccg gtggctacgc cgtcatccac gacaataaca gttttatgcc ggtaagacgg
     4861 aatttggccg gtcccacgaa gcttaataag tttttgttta atttcttttt taagaattat
     4921 agcctgctgg tcaatgtaat cttggctgat attaaataaa ttaagctgat cctgatcaat
     4981 taaaacctgg ccgtccatgt tgactgaacc gatggccagc tccggatcgc ccggagcgcc
     5041 tatttttttg ctaacaataa tatcaagggg cagctttaat tttttagcga cctcatagcc
     5101 agtaaccagg ccgccccggg gcagggcaat aaccacggtt tcagggtttt tcttgtattt
     5161 tttaagctgg tcagctaatt ttttgccggc gtcaggccga tctttgaaat acattaagta
     5221 agattaaagt tgtaagattt aagattaaag ttttgcaaaa aaagataacc tgaactgcca
     5281 taacttaaat cctaaatctt aaatctttaa tctattaagt aaattgcccg accaaatggc
     5341 caacgccgaa tggcgcttcg taagatattt cctcaaattt aggtttcttt tcagttaaaa
     5401 cccccaggag aattataatt gaccgcaggc cgcattcagc cgcgtctttt gaaagctcgg
     5461 gatcaatttt agtaatttct tcaaaagtgt tatctttcag caaagtaagc aatttttgat
     5521 caaattcttt gccttttaga ttataaccgc ccggagcatc ttcagtcaga ctgtgcgaca
     5581 gatcgctgga ggcaatcacc gccacccgtc gccggccaat tttagaaact tcttcactca
     5641 taatttgacc aaactgataa tgcttttgaa aatttaaatt ggagtagcca accggcacga
     5701 tcggcagaat cggctgattg gctgtcaaat aaaataaagg aacagctgtt ccataatcaa
     5761 gcttttgatt gctggttaaa acaatcggca gctccttagt tgatctttcc ctgatttgag
     5821 acaggagttg ctggtcaggc ttaaattcca gctttgtggc aaattctccg aattcctcaa
     5881 aactgcccag gtaatcagaa tttaaattga tggtaaaagc gtcagccagc aggggaccat
     5941 ggggagaaat aacgacaacc gtgtcgggct tagcttcctc aaattttttt ttgagatcgt
     6001 ccagggcctt aatggtagtt ttgaactttt ctatattttc ctggccgata gccgggatta
     6061 gcaaaggaga gtgggggaca atagcggaga aacaaagcat agatatggct aattactaat
     6121 actaatatcg attaattcgc cgtctgggtg gaggtcaagg gctttctgac tggtggtaat
     6181 gacattttcc catttataac caagtttttc
//
LOCUS       DASWHZ010000102         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2449174, whole genome shotgun sequence.
ACCESSION   DASWHZ010000102 DASWHZ010000000
VERSION     DASWHZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449174"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGA49_02285"
     CDS             <1..397
                     /locus_tag="VGA49_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX9664627.1"
                     /translation="SHASLAAKDFFERLYFLFDSNVVNVHTDNGSEFHKHFDAGLKSL
                     ELPHYWSRVRTPKDNPSNERFNRTLKEEFLYWGNFDQDPNIFNRKLTDWLVEYNSIRP
                     HQNLNYLTPLEYAEKTQGLSTMWSSSTST"
     gene            444..1418
                     /locus_tag="VGA49_02290"
     CDS             444..1418
                     /locus_tag="VGA49_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX9664628.1"
                     /translation="MTKLDITCKKILDQINQSIHILVVPHKKPDGDAIGSALAMSLLL
                     DQLGKRHRVFCLDPVSQPFQFLPEWDRVETDPKIFREKRFDLIVVLDSGDLNYSGIDR
                     YLEKIDYPRPAIINIDHHSTSEFFGEINLVLADYSSTCQVLHDFIRKSNLILTKDIAT
                     CLLTGIITDTGGFTNPATTFDSLETASQLLLAGAKFYKINYNILKNKSIPSLKLWGNI
                     LSRLQKNEQSGIVSTFITLDDLDQYHVSDEALDGLANFLNNLDGARAVLVIKEHYNNK
                     IKASLRTSDENLDVSRFAKIFGGGGHKKAAGFTIDGRLIKNGDEIMIA"
BASE COUNT          441 a          338 c          314 g          418 t
ORIGIN      
        1 atcacatgcc agcctggccg cgaaagattt ttttgaacgt ctgtactttc tttttgacag
       61 taatgtggtg aatgtccata cggacaacgg cagtgaattt cacaaacatt ttgatgccgg
      121 tttaaaaagt ttagagcttc cgcattactg gagtcgagtc cgaacgccca aagacaatcc
      181 atcgaatgag cgtttcaatc gtacgctgaa agaagaattc ctttactggg gaaactttga
      241 ccaagatccg aatattttta atcgcaagtt aactgattgg cttgttgagt acaactctat
      301 tcgtcctcat caaaacctca attacctgac acctctggaa tacgcggaaa aaactcaggg
      361 gttgtctacg atgtggtcat ccagtacatc cacttgacaa gccctggtta agccggtaaa
      421 tttatatgat ttagcaaaaa gatatgacca aacttgatat cacctgcaaa aaaatcctgg
      481 accaaatcaa ccagtctatt catattctgg tagtccccca caaaaaaccg gacggcgacg
      541 cgattggatc ggctttggcg atgtcgctgc tgctggatca gctcggcaag cgccaccggg
      601 ttttttgcct tgatccagtc agccagccat ttcagttttt accagagtgg gaccgggttg
      661 aaacagatcc caaaattttc cgggaaaaaa gatttgattt gattgttgtt ctggattcag
      721 gcgatttaaa ctattcaggg attgacagat atctggaaaa aattgattat ccccggccgg
      781 cgattattaa tattgaccat cactcaacca gcgaattttt tggcgaaatt aatctggtgc
      841 tggccgacta ttcttcaacc tgtcaggttc tccacgattt tatccggaaa tcaaatttaa
      901 tactgactaa agacatcgcc acctgtttat tgaccggcat cattactgac accggcggtt
      961 ttaccaaccc ggcgactact tttgattcgc tggaaacggc ttctcaactt ttgctggccg
     1021 gcgctaaatt ttataaaatt aactacaata ttttaaaaaa caaatcaatt cccagcctga
     1081 agctctgggg caatatctta tcacgactcc aaaaaaacga acagtccggc atagtcagca
     1141 cttttattac tctggatgat ttagatcaat atcatgtcag cgacgaagcc ctggacggcc
     1201 tggccaattt tctcaataat cttgacggcg cccgggctgt cctggtcatt aaagaacatt
     1261 ataataataa aatcaaagcc tcgctccgga caagcgatga aaacctcgat gtctcccggt
     1321 ttgccaaaat ttttggcggc ggcggccata aaaaagcagc cggctttacg attgacggcc
     1381 ggctgattaa aaacggagat gaaataatga ttgcgtagaa aggactggaa gttaggttat
     1441 ggttaagagg ccagttttgt ttagcggtta ggaaattagt attctaactt cttaacccaa
     1501 ttaccataca g
//
LOCUS       DASWHZ010000103         9012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3194764, whole genome shotgun sequence.
ACCESSION   DASWHZ010000103 DASWHZ010000000
VERSION     DASWHZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3194764"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..3801
                     /locus_tag="VGA49_02295"
     CDS             430..3801
                     /locus_tag="VGA49_02295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9664629.1"
                     /translation="MQKNNSRKYFTKLHEIIAVPDLIEVQTKSYKWFLEEGLKELFEE
                     VSPIKDFTGRDLELYLLDYYLDEPKFDEKTSREKNVTYEAPLRVTGKLVNKKTGEIKE
                     QEIYIGDFPLMTDRGTFIINGIERAVVSQLIRSAGVFFTSDSHRGRRYYGAKIIPNRG
                     AWIELETDSNNILWAKIDRKRKVAVTSLLRAFGYGADDELLELFKEVDTNPNVKYIQN
                     TIEKDAASSEEEGLKEVYKRIRPGDLATTDNARSLIYSMFFNFDRYDFGRVGRYKINL
                     RFGFDIPNNPETRILRREDLISIIKEIIDLNNSQRDPDDIDHLGNRRVRAIGELVQNR
                     FRVGLARMERIIKDRMSTYDITTITPNRLINARPVIGVVKEFFMSSQLSQFMDQTNPL
                     SELEHKRRLSAMGPGGLSRERAGFDVRDVHQTHYGRICPIATPEGPNIGLVGHLANYA
                     KVNDFGFIETPFRKVIHDVANKVDLLTDKIARGDITDPDGGQVLVKDGQTISEADAKK
                     IAATSLNKISVKPQVLDEVSYLDAFTEEKHITTTATTPIDDNGYFLVEKAEVRRYGRP
                     GTEYVYNIDFMDVSSKQIFSVATSLIPFVEHDDANRALMGTNMQRQAVSCIKPAAPII
                     GTGMEASIAENSGQLILAEDDGEVVEVDGAHISLRLKSGEIKRYDLNKFVRSNASTCM
                     NQHPVVNLGDQVTKGQVLADGASTDQGELALGQNILVAFMSWEGGNYEDAILISERVV
                     HKDFYTSIHIEDYSIDVRDTKLGPEVVTSDIPNVSEEKLKDLDEEGIIRIGAQVSSGD
                     ILVGKITPKGETELSPEEKLLRVIFGEKAKDVRDSSLYLEHGEHGKVVDIKIFSREAG
                     DKLPSGVMKTFQVSIAQLRKVQVGDKMAGRHGNKGVISRVVSVEDMPFLEDGTPVDII
                     LSPLGVVSRMNLGQILETHLGMAARRLGYTAASPSLDGVPEEIIRQELAKAGYQEDGK
                     FTLYDGRNGQVFDSQVTVGQIYMLKLNHLVEDKIHQRSIGPYSLITQQPLGGKAQFGG
                     QRFGEMEVWALEAYGAAHILQEMLTIKSDDVLGRSKAYEAIIKNEPIKKLNVPESFNV
                     LVRELKGLALNVELLDDKGQEIEEVQEPEQLRKEN"
     gene            3914..7780
                     /gene="rpoC"
                     /locus_tag="VGA49_02300"
     CDS             3914..7780
                     /gene="rpoC"
                     /locus_tag="VGA49_02300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX9664630.1"
                     /translation="MFSPIKTTDFRGIRLKLASTEDILDWSHGEVLKPETINYRTQRP
                     EKDGLFCEKIFGPSKDWECYCGKYKKIRYKGIVCDKCGVEVTRSIVRRERMGHIKLSV
                     PVSHIWFLRGVPSKIGLILDMSIQNLEKVIYFANFIITGVNQELKEQTLEQIKNEYKG
                     KNKLIEAEFIQQAENLKNKKSAGAGLSKAEEMKISQLKELDEIFAIAEKELKDLKPLQ
                     IISESAYQDLSLKYGHVFEASIGAEAIRAILEKIDLKETISQLEKELKKATGTKKEKL
                     IRRIKLIKSLEKNKIRPEWMILEVIPVIPPDLRPMVPLDGGRFATSDLNDLYRRVINR
                     NNRLKQLIELKAPEVITRNEKRMLQEAVDALIDNGARRGKTVTASTGQKRMLKSLADM
                     LKGKQGRFRQNLLGKRVDYSGRSVIVVGSRLKLHQCGLPKKMALELFKPFIISRLIRD
                     GLVHNVRSATRYIESDRPEIWDILEDITKEAHVLLNRAPTLHRLGIQAFAPVLIEGKA
                     IKIHPLVCTAFNADFDGDQMAVHLPLTEDAKKEAKELMLSSKNLLKPATGDAITAPTK
                     DMVWGTHYMTYINEEEEATEYFSSPKEAIVKHNLGLLPLRKKIKVRFNDNKMTGLSET
                     SVGRILFNQILPAELPFVNSLVDSKELKALVRAAIKQIGIEPSSKLLDDVKELGFEYI
                     TRSGLSWGMDDIPVLTDKPEIIREAEKRVDEIQEQYETGLLTQSERYTKIIEIWTEVK
                     DKIVKIARQALDKEGSIFTMVESGARGSWGQLTQIVGMKGLVTNPAGEIIELPVRGSF
                     KEGFDILEYFISTHGARKGLSDTALRTANAGYLTRRLIDVAQDVFIHQEDCQTSEGTE
                     VTKQESREIGKDLAERIIGRYAAETIKDPGTGDILFKKNQLIVEERLEDLRKIDLDKV
                     KIRSLLTCRAIKGVCQKCYGLDLAQNKPVDLGTAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVVGADITQGLPRVEEIFEVRSPKRKSIVSEVDGQASVSDVAVVSGKETNEEEALKVQ
                     SVRQKLVRISYKGLKEDVQPVPDKAQVRVKDGVRVSRGDVLLVSADDQEIKAQKGGLI
                     KLEDKEIKVVYEADLVKEYLVPIGYNIWVKDGDLVVKGQQLTEGDVDLHQLFNLRGAD
                     ETQRYIIKEIQRIYSSQGQKLNDKHIELVARQLFSRVYVKTPGDTNLLPGEIVERAEL
                     EEENARVTAEKGQPAEAEQLLLGVTKVSLSTRSFLSAASFQETSKVLINAAITGKIDW
                     LEGLKENVIIGRLIPVGSGFREPTEE"
     gene            7910..8857
                     /locus_tag="VGA49_02305"
     CDS             7910..8857
                     /locus_tag="VGA49_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX9664631.1"
                     /translation="MKKTRKTICLIFCGGSNLADKPAMDISVRSKADISRWLKLVPEI
                     NLMASIEPEFLVSDQADITSSVWEKLAGLIKKKISRVDGLVVTHNLDNILFTSSMMAY
                     IFQNLGKPIVFTGAQLLPEFGLKGNLINAVQVATMNLSEVCLMFGNRLIRATRAVRDD
                     YGSLNSFSADDAGLIGKVDFGVSLQPGVSQNKSRPLKFKNKTDNKVLVTSFYPGSDCR
                     LIEKISCQQRGLFILNDNLSLPGEIEKLADKLATDNIPVVLFNPAGRPAVSTDGNIIK
                     LAGWTYPAALTKFFWVLGQTKQLKQIKNLMLTNLAGEFD"
BASE COUNT         2558 a         1856 c         2251 g         2347 t
ORIGIN      
        1 cagtgcaatt taaaaatttc aaattacaaa atccaaattg ttccaaatca aatccaaaat
       61 ctcccgatgg cgaagccatc gaggatcctc gacaaagtcg ggacaaaatc caaaaatttt
      121 gacatttggg ctttggattt caattgaaat ttgagttttg atatttggat tttatagttg
      181 gattttgaca ttgacatttt aatttctgtg tgtttcctgt taataactcc tgctaatctt
      241 agccaaaaga acttaatttt cagcggattt gcttaatact tgacaaaaat ataagatatc
      301 tgctaatata attaagttac tcaatccctt taaatctggg gaaaactgaa tgaaaagctt
      361 cgcaaaatta ttttaatcgg ctaaacagac aactcaccct tccaacgcgg ggcgggctgt
      421 tttttgtgta tgcaaaaaaa taattcccgt aaatatttca ccaaactgca tgaaattatc
      481 gcggtgcctg acctgattga ggtccagacc aaatcttata aatggttttt ggaagaaggg
      541 ctgaaagaat tatttgaaga agtttcgcct attaaggatt tcaccggccg cgatcttgag
      601 ctttatttgc ttgattatta tctggatgag cccaagtttg acgagaaaac ctcccgggag
      661 aaaaacgtca cttatgaagc cccgcttcgg gtcactggca agctggttaa taaaaaaacc
      721 ggcgaaatta aagaacagga aatttatatt ggcgattttc cgctaatgac cgaccgggga
      781 acttttatca ttaacggaat tgagcgggcc gtggtttccc agctgatcag gtcagccggg
      841 gtttttttta cttcagacag ccaccgggga cgccgctatt acggcgccaa gattattccc
      901 aaccggggcg cctggattga actggaaact gattctaata acattctctg ggccaagatt
      961 gaccggaaaa gaaaagtagc ggttacttca ttgctgcggg cttttggcta tggcgccgat
     1021 gacgaattgc tggaactttt taaagaagtt gataccaatc ccaatgttaa atatattcaa
     1081 aatacaattg aaaaagacgc ggcttcttca gaggaggaag gcctgaagga agtttacaaa
     1141 agaatccggc cgggtgatct ggcgacaact gacaacgccc ggtcgttaat ttattcaatg
     1201 ttttttaatt ttgaccggta tgattttggc cgggtcggcc gttacaaaat taatttaagg
     1261 tttggctttg atattcccaa caatcctgaa accagaattc ttcgccggga agacctgatt
     1321 tccattatca aagaaatcat tgatcttaac aacagccagc gtgatccgga tgatattgat
     1381 catctgggca accgccgggt ccgggcgatc ggcgagctgg ttcaaaatcg gtttcgcgtc
     1441 ggcctggctc ggatggagcg gattatcaaa gaccggatga gcacctatga cattacgacc
     1501 attaccccca accggctgat caacgcccgg ccggtgattg gcgtggttaa agagtttttt
     1561 atgtccagcc agctgtcgca gtttatggac cagactaatc ctttgtcaga gctggaacac
     1621 aagcggcggc tgtcagccat gggtccgggc gggttgtcca gagaacgagc cggttttgac
     1681 gtccgggacg ttcaccagac tcattacggc cggatttgtc cgattgccac cccggaaggc
     1741 cccaacattg gtttggtcgg tcatttggcc aactacgcca aagttaacga ttttgggttt
     1801 attgaaactc cttttagaaa agttattcat gatgtcgcca acaaagttga tttgctgact
     1861 gacaaaattg cccggggtga cattaccgac cctgatggcg gacaggtttt ggtcaaagac
     1921 ggtcagacta tttcagaagc tgacgccaaa aaaattgccg cgaccagtct taataaaatt
     1981 agtgtcaagc cccaggtact tgatgaagtc agttatctgg atgcttttac ggaagaaaaa
     2041 catattacca ccacggccac cacgccgatt gacgacaacg gttatttttt ggtggaaaaa
     2101 gccgaggtca gacgctacgg ccggccaggc actgaatatg tttacaatat tgattttatg
     2161 gatgtttcat ccaaacagat tttcagcgtg gctacttcgc tgattccttt tgttgagcat
     2221 gatgacgcca accgggcttt aatgggcacc aatatgcagc gccaggcggt ctcctgcatt
     2281 aaaccggcgg cgccaattat tggcaccggt atggaagcgt ccatcgccga aaattccggt
     2341 cagctgattt tggcggagga cgacggcgaa gtcgtggaag ttgacggagc tcatatttct
     2401 ctcaggctaa aatccggtga aatcaagcgt tacgatttaa ataaatttgt ccggtcgaac
     2461 gcttccacct gcatgaacca gcatccggtt gttaatttgg gcgatcaggt cacaaaaggc
     2521 caggtgctgg ctgacggcgc ttcgaccgac cagggcgagc tggctctcgg ccagaatatt
     2581 ttggtggctt ttatgtcctg ggagggcggc aactatgagg acgctatttt aatttctgaa
     2641 agagtagttc ataaagattt ttatacttcc attcatattg aagattattc aattgacgtc
     2701 cgggacacca agctggggcc ggaagttgtt acctctgata tcccgaatgt cagcgaagaa
     2761 aaactgaaag acctggatga agaaggcatt atccggattg gcgcccaggt ctcatccggc
     2821 gatattttgg ttgggaaaat tactcccaaa ggagaaactg aactatcgcc cgaagaaaaa
     2881 ttgttgcggg taatttttgg tgaaaaggcc aaagacgtcc gggacagctc tctttatctg
     2941 gagcacggcg aacacggcaa agtggttgat attaagattt tttcccggga agccggcgat
     3001 aaattgccgt caggagtcat gaaaactttt caggtgtcaa tcgcccagtt aagaaaagtc
     3061 caggtgggcg ataaaatggc cggccgtcac gggaacaaag gcgttatttc cagggttgtt
     3121 tcagtggaag acatgccttt tctggaggac ggcacaccgg ttgatattat tttaagtcct
     3181 ttaggggttg tttcccgaat gaacctgggg caaattttgg aaacccatct ggggatggcc
     3241 gccaggaggt taggctacac cgcggccagt ccgtctctgg acggcgtgcc cgaagaaatt
     3301 attcgtcagg aactggccaa ggccggctat caggaagacg gcaagtttac tttatacgac
     3361 ggacggaacg gtcaggtttt tgacagccag gtgactgttg gtcagattta tatgctgaag
     3421 ctgaaccatt tggttgaaga taaaatccac cagcggtcta ttggccccta ctcattaatt
     3481 acccagcagc cgctgggcgg caaagcccag tttggcggtc agcgttttgg agaaatggaa
     3541 gtctgggctt tggaagctta cggcgccgct catattttac aggaaatgct gactattaaa
     3601 tccgatgatg ttttgggccg gtccaaagct tacgaggcca ttattaaaaa tgaaccaatt
     3661 aagaaactta atgttccgga atcatttaat gttttggtca gggagctgaa aggcctggct
     3721 ttaaatgttg aactgctgga cgacaaaggc caggaaattg aagaagtcca ggaacctgaa
     3781 cagttaagga aggaaaatta aatatctagg ggatagggga tagggtatag gttatagggt
     3841 atagtgagtt gaaaaaaaat ttcattatcc tattaattta ttccctatcc cctatcccct
     3901 ataccctaac tatatgttta gtccaattaa aacaactgat ttcaggggca tccggctaaa
     3961 actggcttca acagaagata ttttagactg gtctcacggt gaagttttaa agccggaaac
     4021 tattaattac cggactcaac ggcctgaaaa ggacggactt ttttgtgaaa agatttttgg
     4081 gccgtccaag gactgggagt gttactgcgg caaatataaa aaaatacgct acaaaggcat
     4141 tgtttgcgac aagtgcggcg tggaagtcac ccggtcaatt gtccggcggg aacgaatggg
     4201 gcatattaaa ctatcggtgc cggtttccca tatctggttt ttacggggcg tgccttcaaa
     4261 aattggtctg attcttgaca tgtcaattca aaatttggaa aaagttattt attttgccaa
     4321 ttttattatt accggcgtca accaggagct gaaagagcag actctggagc agattaaaaa
     4381 tgaatataag ggcaaaaata aattaattga ggcggaattt atccagcaag cggagaattt
     4441 aaaaaataag aaatcagccg gagcgggact gtctaaagcc gaagaaatga aaatcagcca
     4501 actgaaagag ctggatgaaa ttttcgctat tgctgaaaaa gagctgaaag atttaaagcc
     4561 tttacaaata atttctgaaa gcgcttatca ggatttatca ctgaaatacg gccatgtttt
     4621 tgaagccagc atcggcgctg aagctatcag ggctattttg gaaaaaattg atttgaaaga
     4681 aactattagc cagctggaaa aagagcttaa aaaagccacc gggaccaaaa aagaaaagct
     4741 gatcagacgc atcaagctga ttaagagttt ggaaaaaaat aaaattcgtc cggaatggat
     4801 gattttggaa gttattccgg ttattccgcc tgatttaagg ccaatggttc cgctggatgg
     4861 cggccggttc gccacttctg atttaaatga tttataccgc cgggtgatca accgcaacaa
     4921 ccggctaaaa cagctgattg agctcaaagc cccggaggtt attacccgaa atgaaaaaag
     4981 aatgcttcag gaagcggttg acgctttgat tgacaatggg gcccgccggg gcaaaacagt
     5041 caccgcctca accggtcaga aaaggatgct gaaatcgctg gccgacatgc tcaaaggcaa
     5101 gcagggtcgt tttcggcaaa atttgcttgg caagcgggtt gactattccg ggcggtcagt
     5161 tattgtcgtt ggttcgcgtt taaaacttca tcagtgcggc cttcctaaaa agatggccct
     5221 ggagctgttc aagccgttta tcatcagccg gctgattcgg gacggcctgg ttcataatgt
     5281 ccggagcgct acccgctata ttgaaagtga ccggccggaa atttgggata ttttggaaga
     5341 tattaccaaa gaagcccatg ttttgctcaa ccgggcgccg actcttcatc gtctcggtat
     5401 tcaggccttc gcgccggtgt taattgaagg caaagcgatt aaaattcatc cgttggtctg
     5461 taccgctttt aacgctgatt ttgacggcga tcagatggcc gttcatctgc ctttgaccga
     5521 agacgccaaa aaagaagcca aagagttgat gctgtcgtcc aaaaatttgc tcaagccggc
     5581 gaccggagac gccattaccg ctccgactaa agatatggtc tggggaactc attacatgac
     5641 ttatattaat gaagaggagg aagccactga atatttttct tcacctaagg aagcgattgt
     5701 taaacacaat ttaggcttgc tgcctttaag aaaaaaaatt aaagtcagat ttaatgacaa
     5761 taaaatgacc ggcttgagtg aaacttccgt tggccggatt ttattcaacc aaattctgcc
     5821 ggccgaactg ccgtttgtca attccctggt ggattccaaa gagctaaaag cgcttgtccg
     5881 ggcggccatt aagcaaatcg gcattgaacc gtccagcaaa ctgctggatg acgtcaaaga
     5941 acttggtttt gaatatatta cccggtcagg tttatcctgg ggaatggatg atatcccggt
     6001 tctgactgac aagccggaga ttatcaggga agctgaaaag cgggttgacg aaattcagga
     6061 gcaatacgaa acaggcttat taactcaaag tgaacgctat actaaaatta ttgaaatctg
     6121 gacagaggtt aaggacaaaa ttgttaaaat cgcccgtcag gcgctggata aggaagggtc
     6181 tatttttaca atggttgaat caggcgcccg gggttcctgg ggtcagctga cacagattgt
     6241 cggcatgaaa ggcctggtga ccaacccggc cggtgaaatt attgaattgc cggtcagggg
     6301 aagtttcaaa gaaggttttg atattctgga atatttcatt tcaactcacg gcgcccgaaa
     6361 gggtttgtct gacaccgctt taagaacagc caacgccggc tatttaaccc ggcggctaat
     6421 tgatgtcgcc caggatgttt ttattcatca ggaagactgc cagacttcag aagggactga
     6481 agtcaccaaa caggaaagcc gggaaatagg caaagatctg gccgaacgga ttattggccg
     6541 gtacgccgct gaaaccatta aagatcccgg gaccggcgat attttgttta aaaagaatca
     6601 gctgattgtc gaggaaagac tggaagattt aagaaaaatt gatctggaca aagttaaaat
     6661 aagatcgctt ttgacctgcc gggccatcaa gggagtttgt cagaaatgct acggacttga
     6721 tctggctcaa aataaaccgg ttgatttggg aacagccgtt gggattatcg ccgctcaaag
     6781 cattggggaa cccggcaccc agctgacaat gagaactttc cataccggcg gcgtggtcgg
     6841 cgctgacatc acccagggac tgccccgggt ggaagaaatt tttgaagtcc ggtcgcccaa
     6901 acgaaaatca attgtcagcg aagttgacgg ccaggcttcc gttagcgacg tggccgttgt
     6961 cagcggtaaa gaaaccaatg aagaagaagc tttaaaagtt cagtcggtta gacaaaaact
     7021 tgtcagaatc agctacaaag gtctgaagga agatgttcag ccggtgcctg acaaagccca
     7081 ggtcagggtt aaagatggcg tcagggtcag ccggggtgat gttttgctgg taagcgctga
     7141 cgaccaggaa attaaagccc agaaaggcgg cctgattaag cttgaagata aagagatcaa
     7201 agttgtttat gaagctgatc tggttaaaga gtatctggtg ccgattggtt ataatatctg
     7261 ggttaaagac ggcgatctgg tcgtcaaagg ccagcagttg accgaaggag atgttgacct
     7321 gcatcagctt ttcaatttaa gaggcgctga tgaaacccag cgttacatta ttaaggaaat
     7381 tcagagaatt tattcttctc agggacaaaa gctgaacgac aagcatattg aactggtcgc
     7441 ccgccagctg ttttccaggg tttatgttaa aactccgggg gacactaatt tattaccggg
     7501 agaaattgtc gagcgggccg agctggaaga agaaaacgcc cgggtaaccg ctgaaaaagg
     7561 ccagccggct gaagccgaac agctgttact gggagtcact aaagtgtctc tgtcaaccag
     7621 aagcttttta tcggccgctt ctttccagga aacttccaaa gttttgatta acgccgccat
     7681 caccggcaag attgactggc tggagggact gaaagaaaac gttattattg gccggctgat
     7741 tccggttggc agtggcttca gagagccgac tgaagaataa aaattgctga cttgcggaag
     7801 ataagctctc ccggaccccg ccgctcgcga gcggcggggt tgacggggga gttttaaaat
     7861 agtaaattta tcagagagcc gtcttaaagt ttagagccgt ctttgattta tgaaaaaaac
     7921 cagaaaaaca atttgtttaa ttttttgcgg cggcagcaat ttggctgaca agccggccat
     7981 ggatatttct gtcaggtcca aagccgatat ttcccgctgg ctgaaactgg ttccggaaat
     8041 taatttaatg gcttcgattg agccggaatt tttagtcagc gaccaggcgg acattacttc
     8101 gtcagtttgg gaaaaattag ccggtttgat taagaaaaaa attagccggg ttgacgggct
     8161 ggtcgttacc cataatttgg ataatatttt gtttacttct tcaatgatgg cttacatttt
     8221 tcaaaatctg ggtaagccga ttgtttttac cggcgcccag ctattgccgg aatttggcct
     8281 gaaaggcaat ctaattaacg ccgttcaggt ggccaccatg aatttgtcag aggtttgtct
     8341 gatgtttggc aaccggttga tcagggccac ccgggctgtc cgggatgatt acggttcgct
     8401 caatagtttt tcggccgacg atgccggact gatcggcaag gttgattttg gcgtttcgct
     8461 tcagcctggc gtcagtcaaa acaagagccg gccgcttaaa tttaaaaata aaactgacaa
     8521 taaggttctg gttaccagtt tttatccggg gagtgactgt cggctgattg aaaaaataag
     8581 ctgtcagcaa cggggtctgt ttattcttaa tgacaatttg tcactgccgg gggaaattga
     8641 aaaactggcc gacaagctgg cgactgacaa tattccggtg gttttattta acccggccgg
     8701 ccggccggct gtctccacgg acggtaacat tattaaatta gccggctgga cttatccggc
     8761 ggctttgacc aagtttttct gggttttggg ccagactaaa caattaaagc agattaaaaa
     8821 tttgatgctg actaacttag ccggtgaatt tgattaaagt tatgagattt tatttatttt
     8881 tttccaaaat gggacaatca gccgaaacat ttaacgctaa gctgactgaa attttaggca
     8941 agtccggcat taattttatt tctaatctgg agccagccaa aatttctgat tataatacca
     9001 gtgacctggc ta
//
LOCUS       DASWHZ010000104         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2459886, whole genome shotgun sequence.
ACCESSION   DASWHZ010000104 DASWHZ010000000
VERSION     DASWHZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459886"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VGA49_02310"
     CDS             complement(<1..893)
                     /locus_tag="VGA49_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX9664632.1"
                     /translation="MPTRVAINGFGRIGRTTFKAGFGLKNLDWLAVNDLTDPKTLAHL
                     LQYDSVYRTFDKKVQYNEEKKKIGGCQCQGRLIVGNKKIMIFSEKDPSKLPWKELKID
                     VAIESTGVFTEKKQAGQHLKAGAKRVIISAPAKGDQPVQTYMRGVNLDKYQDEAIIDN
                     ASCTTNCASPVAAVIHQKFGVQKALMSTVHSVTADQRLVDAPHKDLRRARAAYVNIVP
                     TTTGAAIATTKAIPDLEGKFDGMSIRVPVIDVSLVDFTFLLKKKTTVEEVNRYFKKMS
                     QHPLYKGILGVSEEDLVSSDY"
     gene            complement(906..1394)
                     /locus_tag="VGA49_02315"
     CDS             complement(906..1394)
                     /locus_tag="VGA49_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664633.1"
                     /translation="MNVNQKGFSATTVAVAVIIIIILFGGLAYYFTLPASQPADQPLD
                     QVQYKTYFSQRDKYEVQIKESWTAEEDETGTTFLDESGQVQIVVIRQPDIPGRPLLPE
                     LIEDITVAGVQGTIYHDYSQTDQRYVDKVIFDVPDSEDDIYIAGLGDDFSQFIKDFQL
                     TD"
     gene            complement(1619..2197)
                     /locus_tag="VGA49_02320"
     CDS             complement(1619..2197)
                     /locus_tag="VGA49_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9664634.1"
                     /translation="MDMKIISLLRQQTGAGVLECKAALEEAGGDLDQAVEILRKKGAD
                     KAAKKMERETKEGAIGNYIHSNKKIGAMVEVLSETDFVARNEEFQELTHDLAMQVAAQ
                     NPLYLRPEDIPDEVLVKEKDIYLEQLADEVKPEMLDKVIEGKIDKYYEDVCLLNQKFI
                     KDDTKTIEDLINEKIAKIGEKIEIRKFCRFEI"
     gene            complement(2258..3091)
                     /gene="rpsB"
                     /locus_tag="VGA49_02325"
     CDS             complement(2258..3091)
                     /gene="rpsB"
                     /locus_tag="VGA49_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9664635.1"
                     /translation="MPEIPSMTEMLKAGVHFGHKASKRYPKMEPYIYTVRSGIHIINL
                     EKTTAKLKEALGFVTETVAKGGTVLFLGTKKQARDIIKKYAVDCQMPYISERWLGGLI
                     TNFPVVVKVIKKYNKMKADRQAGAYAKYTKKEQLEFDREIERLDRMVGGLASLTKIPE
                     IIYIVDIRNENTAVAEANNKKVPIVAICDTNVNPEKVSYPIPANDDSIGSIEMITRLI
                     SEAVKEGQKQSTVSSQQPTGKEQEIVSPAGNASGTGSQPAQPAEKKEAAKPAEKTTIK
                     E"
BASE COUNT          884 a          740 c          647 g         1084 t
ORIGIN      
        1 atgtagtccg aagacaccag atcttcctct gatactccca aaattccttt ataaagagga
       61 tgttggctca tttttttaaa atagcgattg acctcttcaa ccgttgtttt tttctttagc
      121 aaaaaagtga aatcaaccaa agaaacatca atcaccggga ctctgataga catcccgtca
      181 aatttacctt ccagatcagg aatggctttg gtagtggcaa tggccgcgcc ggtggtcgtc
      241 ggcacgatat tgacataggc cgcccgggcc cgcctcaaat ctttgtgcgg ggcgtccacc
      301 agccgctgat cggcggttac tgaatggact gttgacatta acgctttttg aacgccaaat
      361 ttctgatgaa tgaccgcggc caccggcgaa gcgcagttgg tcgtgcagga agcgttatca
      421 atgatggcct catcctgata tttatccaga ttaactcccc gcatataagt ctgaaccggt
      481 tgatcgcctt tggccggagc tgaaataatc acccttttgg cgccggcttt aagatgctgg
      541 ccagcctgtt ttttttcagt aaaaactccg gttgattcaa tcgccacgtc aattttgagc
      601 tctttccagg gcagtttgga cggatctttt tcactgaaaa tcattatttt tttattgcca
      661 acaatcagcc ggccctgaca ctggcagccg cctatttttt tcttctcttc attatattga
      721 acttttttat caaatgtccg gtaaacgctg tcgtactgaa gtaaatgagc cagagttttg
      781 ggatcagtca gatcattaac cgccagccag tctaaattct ttaaaccaaa accagcttta
      841 aaagtcgtcc ggccaatccg gccgaaaccg tttatggcaa cgcgcgttgg catatttatt
      901 tttgtttaat cagttaattg aaagtctttg ataaattgac tgaaatcatc gccaagtccg
      961 gcgatataaa tgtcgtcttc agaatcagga acgtcaaaaa ttactttatc aacataacgc
     1021 tgatctgttt gggagtagtc gtgataaatc gtgccctgaa cgccggcgac agtaatatct
     1081 tcaatcagct ccggcagcag cggccggccc ggaatatccg gctgtctgat aacgacgatt
     1141 tgaacctggc cgctttcatc taaaaacgtg gtgccggtct catcttcttc agcggtccag
     1201 ctctctttaa tctgaacttc gtatttatcc cgttggctga aataagtttt atattgaacc
     1261 tgatccaggg gctgatccgc cggctgactg gccggcagag tgaaataata agccaggccg
     1321 ccaaaaagaa taatgatgat aatcacggca accgcgactg ttgtggctga aaagcctttt
     1381 tgattaacat tcatatcgtt aatttatttt aacattagca aaaattgaaa gcaagatcat
     1441 taaattctaa tatctaaatc ataaatccta aacaaattat aaatcttaat gttctaaatc
     1501 ccaaacactc tgtataaaat ttttttgaaa cattggaatt tataatttaa aatttgttta
     1561 gaaattagat attagaaatt agaaatttta aaagcggaga cttgctccgc tttgttgact
     1621 atatttcaaa tcgacaaaat ttcctaattt ctatcttttc tccaattttc gctatttttt
     1681 catttatcaa atcttctatc gttttagtgt cgtctttaat aaatttttgg ttcagcaaac
     1741 agacatcctc gtaatattta tcaatttttc cttctatcac tttgtccagc atctccggct
     1801 tgacttcatc ggccagttgc tccaggtaaa tatctttttc tttgaccagg acctcgtcag
     1861 gaatatcttc cggtcttaaa tataaagggt tttgagcggc cacctgcatg gccagatcgt
     1921 gggtcagttc ctgaaactcc tcattgcggg cgacaaaatc ggtttctgac aaaacttcga
     1981 ccatcgctcc gatttttttg ttggaatgaa tataattgcc aatggcgcct tccttagtct
     2041 cccgctccat ttttttagcg gctttatcag cgcctttttt tcttaaaatt tcaacagcct
     2101 gatcaagatc gccgccggct tcttccaggg cggctttaca ttccaggacg ccggcgccag
     2161 tttgttgtct taataaagaa ataattttca tgtccataat attataagtt aaaagtaaaa
     2221 agtaagaagt aataagtaag gaataagaaa taaaaaatta ttctttgata gttgtcttct
     2281 ctgctggttt tgcggcttct tttttctctg ctggctgggc gggttgactg cctgtcccgc
     2341 tggcattgcc ggcggggctg actatttcct gctctttccc tgttggctgt tgactgctga
     2401 ccgttgactg cttctgccct tccttaacgg cctcactgat caatcgggtt atcatttcaa
     2461 ttgaaccaat cgaatcgtca ttagccggaa tgggatagct gactttttcc gggttaacat
     2521 tggtatcgca aatagcgaca atcggcactt tcttgttatt agcttcggcc acggccgtat
     2581 tttcatttct gatgtcaaca atgtaaataa tttcaggaat tttcgtcaga ctggccaaac
     2641 cgccgaccat tctgtccagc cgctcaattt ccctgtcaaa ctccagctgt tcttttttag
     2701 tgtatttggc gtaagcgccg gcttgacgat cagccttcat tttattatat tttttaatta
     2761 ctttgacaac caccgggaaa ttagtgatca agccgcccag ccagcgttca gaaatataag
     2821 gcatctggca gtcaacggcg tattttttaa taatatctct ggcctgtttt tttgtgccta
     2881 aaaaaagaac agtgccgcct ttagcaactg tttcggtcac aaatcccaaa gcttctttca
     2941 gcttggcagt tgttttttcc agattaatga tatgaatccc gctgcggaca gtataaatat
     3001 acggttccat tttgggatag cgcttggaag ctttgtggcc aaaatggaca ccagctttca
     3061 gcatctcggt cattgatggt atttcgggca taattgtttc ctttcttcct ctactccatt
     3121 ttcttgacta aataatcctc tggcaaggac aagggcttta gtcaatatcc gatcagggaa
     3181 tgaagtatgt gtgataaaaa attaaaaact aacaaatatg atttaattaa aatatcaaaa
     3241 acaatctaaa tagtcaagtg ttgatggtaa ttatttggat ttgtttaatt ttttaatcaa
     3301 atcagccact tcttcaattg tcggccggtc agctttgttt ttttccagca ttcgc
//
LOCUS       DASWHZ010000105         2963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_959903, whole genome shotgun sequence.
ACCESSION   DASWHZ010000105 DASWHZ010000000
VERSION     DASWHZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959903"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VGA49_02330"
     CDS             complement(<1..316)
                     /locus_tag="VGA49_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664636.1"
                     /translation="MSDSEEKRERERQLVALNERLVLAPLGWSLPLTMILVITLVELK
                     VIRFTGSIGLQVFVFILCFLCWIFAFMALELLIFRPWRRRKEKELDDLYKIWKEPCEQ
                     DRQ"
     gene            complement(621..932)
                     /locus_tag="VGA49_02335"
     CDS             complement(621..932)
                     /locus_tag="VGA49_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664637.1"
                     /translation="MKFLRIFFPQKDPIDIKDKYNIPETISWDIQLNKDGLVATSKEL
                     PGLVTNASNPEELLEMINDAVLEYFDVSKLESDYVFDSLDLSGHGEVSLKQAQEKKQY
                     A"
     gene            complement(1025..1150)
                     /locus_tag="VGA49_02340"
     CDS             complement(1025..1150)
                     /locus_tag="VGA49_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664638.1"
                     /translation="MTKTILIVLIVLILGLAIGYYIGYDIGWERAVYFLQSDAAL"
     gene            complement(1161..1247)
                     /locus_tag="VGA49_02345"
     tRNA            complement(1161..1247)
                     /locus_tag="VGA49_02345"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1211..1213),aa:Ser,seq:cga)
     gene            complement(1347..1475)
                     /locus_tag="VGA49_02350"
     CDS             complement(1347..1475)
                     /locus_tag="VGA49_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664639.1"
                     /translation="MQNWYMFYSFGIGGTIVGMIEAAIWGFIGAYVFAWVYNMFVK"
     gene            complement(1541..2203)
                     /locus_tag="VGA49_02355"
     CDS             complement(1541..2203)
                     /locus_tag="VGA49_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9664640.1"
                     /translation="MEGFIEAVYDGYQKLNDDVYEFYLKVDDFDFEPGQFVTFMLKDD
                     QGEFGRSYSIIDYRENQIIFCVKVLDEGRGSQALLKLKKGDVLPITSAKGIFQVQKTG
                     QPKLFVANGVGIAPFIPMIQSIPDAKKTLILGCRYQTDLLYLDKLKKVPNLKLMISLT
                     KPEKDWKGLTGRVTEILEKETVEPETEVYICGSADMIGDVIKHLNSIDHPKKHIFTEA
                     FY"
     gene            complement(2278..2550)
                     /locus_tag="VGA49_02360"
     CDS             complement(2278..2550)
                     /locus_tag="VGA49_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HEX9664641.1"
                     /translation="MRVYPSSRFKRSYKKLSFSIKDDFNSKIKIFFRHPFDSRLKTHK
                     LRGKLDSYYAFYLREGYRVLFVFEDDDNVLLINVGSHDDYARWSKK"
     gene            complement(2554..2793)
                     /locus_tag="VGA49_02365"
     CDS             complement(2554..2793)
                     /locus_tag="VGA49_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664642.1"
                     /translation="MKTKMKKPASVKLPLTALRCLYKTARGKKIVCEIDVAQMKKLNE
                     ATTIDEMVAEARLERAVGKTKFFTSADDLIKELRS"
BASE COUNT          910 a          655 c          510 g          888 t
ORIGIN      
        1 gttgcctgtc ttgttcacaa ggctccttcc atattttgta gagatcatcc agctcttttt
       61 ctttgcggcg gcgccagggt ctaaaaatca gcaactccag cgccataaac gcgaaaatcc
      121 agcataaaaa acagaggata aaaacgaaga cctggagtcc gatactgccg gtaaagcgga
      181 tgactttaag ctccaccagc gtgatcacca aaatcatggt cagcggcaaa gaccagccta
      241 acggggccag caccagcctt tcattcaagg ccacaagctg acgctcacgt tctctttttt
      301 cttcagagtc agacatctct aacctccttt tcaaggtgca gtttgttatc ttagacacgc
      361 tgtctaagtt tcaggttttt aattaaataa gtataactca atgcagtgaa tttttcaatt
      421 tcgcccacaa tcacgtcttg gcaaaaaaat ttatgtcttg ggactgttat aaacggtctg
      481 gtgtttcctt ccaatccggt aacaaattct tgaggcggtt taaatttgta atgctttgct
      541 cttcttttac tggcatccgt aaaacctatt ttaagtaagg ctctaataca ctatttctgc
      601 gtgaaattat aacccatact ctaagcgtac tgcttttttt cttgagcctg ttttaaagaa
      661 acctcgccat gaccagacaa atctaagctg tcaaaaacat agtcagactc aagcttagaa
      721 acatcaaaat attccagaac tgcgtcatta atcatttcca ataattcttc aggattactg
      781 gcgttagtaa ccagccctgg cagctctttg gaagtggcca ccagtccatc tttatttaac
      841 tgaatatccc agctgatagt ctccgggata ttatacttat ccttgatatc aataggatct
      901 ttttgtggga aaaatattct tagaaatttc atttttttat gttatgtata aacgttataa
      961 ctataacata taagcataaa atgatccatc ggtcaagttt gacccaactt ttaatctcat
     1021 aaatttacaa agcggcgtct gactgtaaaa aataaactgc ccgttcccag ccgatatcat
     1081 aaccaatata ataaccaatg gccaggccta aaattagaac aatcaggaca atcagaattg
     1141 ttttagtcat aatctataaa cggagagggc gagatttgaa ctcgcgaggg cggttacgcc
     1201 caaccgcttt tcgagagcgg cgccttaaac cactcggcca cctctccata aatcacattt
     1261 acattaaata atatcacaaa accatcaaaa aagaaaaccc ggaaagtttt tccggatttt
     1321 ctgagactga tactatctgg tcaaaactat ttaacaaaca tattgtaaac ccaggcaaaa
     1381 acataggcgc cgataaagcc ccaaatagcg gcttcaatca tgccaacgat ggtgccgcct
     1441 atcccaaaac tgtaaaacat gtaccagttt tgcatctgcg tggcggcgtt gcggtagata
     1501 tagaaaatat ggccaaataa ataatctttc cttaattata tcaataaaaa gcttcggtga
     1561 aaatatgctt tttgggatga tcaattgaat tcagatgttt aataacatcg ccaatcatgt
     1621 cagcgctgcc gcagatataa acttctgttt ccggctcaac agtttctttt tccaaaattt
     1681 cagtcacccg accggtcagg cctttccagt ctttttctgg tttagtcaaa gaaatcatta
     1741 attttaaatt aggaactttt tttaatttgt ccaaatacaa taaatcggtt tgatagcgac
     1801 acccaagtat caaagtcttt ttggcatcag gaattgattg aatcattgga ataaaaggag
     1861 caatgcccac gccattagcc acaaacaatt ttggctggcc ggttttttga acctgaaaaa
     1921 ttcctttggc tgaagtaatc ggcaaaacat cgcctttttt cagtttcaat aaagcctgac
     1981 tgccccggcc ttcgtccaga actttcacgc agaaaataat ctgattctct ctataatcaa
     2041 taatagaata actccgtcca aattcaccct gatcgtcttt gagcataaaa gtcacgaact
     2101 ggcccggttc aaaatcaaaa tcatccactt ttaagtagaa ttcataaaca tcgtcattta
     2161 atttttgata gccgtcataa acggcctcaa taaaaccttc catagatata gtttagcaaa
     2221 aatattgact tttttgaaaa tctccattgc cccgtgctca ccgcgcatgg ggctttttta
     2281 ttttttggac catctggcat aatcatcgtg actgccgaca ttgatcaaaa gcacattatc
     2341 atcatcttca aaaacaaaaa gcacccgata accttcacgt aaataaaacg cataatagga
     2401 atcaagtttt ccacgcagct tatgtgtttt cagtctggaa tcaaaagggt gcctgaaaaa
     2461 aatttttatt ttgctgttaa aatcatcttt gattgaaaat gataattttt tataagaacg
     2521 tttgaagcgg gaagacggat aaaccctcat aaattatgag cgcagttctt tgatcagatc
     2581 atcggcggaa gtaaaaaatt ttgttttgcc cactgcccgc tctaaccggg cttcagcgac
     2641 catttcatca attgtggtcg cttcgttaag ttttttcatt tgagcaacat caatctcgca
     2701 aacaattttt tttccgcgag cagttttgta taaacatctt aaagcagtca aaggcagctt
     2761 gaccgacgct ggtttcttca ttttagtttt cataattttc agactaatct aaacttataa
     2821 tatatctctg aacgccagaa gtcaaatgat ttatcttgcg ggtaaattag acccccttct
     2881 cccgataatt catttttcac ggcacggaag gggcgagatt ccccgcacca gtcgctacgc
     2941 tcgggtgcgg ggcaggcgtt cca
//
LOCUS       DASWHZ010000106         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_959914, whole genome shotgun sequence.
ACCESSION   DASWHZ010000106 DASWHZ010000000
VERSION     DASWHZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959914"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /gene="trxA"
                     /locus_tag="VGA49_02370"
     CDS             <1..262
                     /gene="trxA"
                     /locus_tag="VGA49_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9664643.1"
                     /translation="LVDFFATWCGPCKIMAPMIEELAKEYQGQKVNIAKLDIDQSRAI
                     VQKFNIMSVPTFKFFKKGQEVGEHIGTSGKEDFKERIDELLK"
     gene            467..1399
                     /gene="trxB"
                     /locus_tag="VGA49_02375"
     CDS             467..1399
                     /gene="trxB"
                     /locus_tag="VGA49_02375"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9664644.1"
                     /translation="MSNNNLNNVIIIGGGIAAHTAAIYTARASLKPLVISAAEPDQLS
                     LTTTVENWPGYVDGVQGPELIADSKKQAQKFGAEYISGQVNSVKKTEEGFDIIAGDKS
                     FQTKTVIIATGASARRLNIPGEDQYFGRGVSTCAVCDAALFRDKDVVVVGGGDSAMEE
                     TLALYKFARSIKLIHRRDAFKASQIMRDRVLGLTDKVEILYSHQALEVLGDGKFVTGV
                     KVKDLKNDKDYEIAVNGMFLAIGHTPNTKFLEGLVELDDQGYIVTDKRSRTSVEGIYA
                     AGDVQDPIFRQAITSAGAGCQAALEAERHIENRQ"
     gene            complement(1691..3991)
                     /locus_tag="VGA49_02380"
     CDS             complement(1691..3991)
                     /locus_tag="VGA49_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664645.1"
                     /translation="MSKSTKIVIGAVVGLIVVIGGVFWYFNRPTVVDTDITLEGDHLI
                     PASEKMILKDGAKLTVNGNFAVEGDLECANGPLNLTVNGALLVRGELKCEQPEDLSGE
                     KLADGINLVVTEGIEFASSAMIITNNSLQLVKSPADLARTKEELDQIYQDAGTDSGAG
                     FRTGPFIDIPLAPGQAASNAGGGENETVLTPRATAKTYQISLVKKLKNILINPVQAQV
                     PAEDFFGWPVNDTVKIGGRWVMGHGQTPPEELGVPIPPKDVDKIILNFNFGANGKIII
                     ENFTLFGPDGRPGDADEGENCAAVGKPGENAFRFRVIAGEITVVSFDLWLGYGGRGGD
                     AETPDNECDPGIATGGQGGEPGNFKMNASNKFEIVGPFNIYPGRGGRGGEATAHGKDG
                     EDNCPGTKGGDAIATGGQGGDNKKGLKVVGTIAGTSNITIHPLYGGEGGRSIASPGKG
                     GSGTGCGCDGGQGGTGTATGGAGGDATSRTVDSFGGDGGDTTALGGFGGDGGHCGPRG
                     PGGNGGNGGDADATEGPAGTGKTGPGEPGVVEEKPGGDGGNGGNGCPEGQGGDGGQGD
                     PAGEPGDPGKRICPEINTNIGIIPVPDESGVLTEPGAGPPTTVPTPQPEPTPTPTPTP
                     APTPTPEPTPEPEPTPEPTPEPTPEPTPEPEPEMPPISASPSSISKVHVYGESNCPDP
                     FQTIALSGPGGQPWSIISSPAWVNFSQSTGVFPGSDPTQSSSVQLPSNFPCSGFTGDN
                     QSGNVRFQSGDSFFDVFFSLEIQGLP"
BASE COUNT         1093 a         1048 c         1139 g         1103 t
ORIGIN      
        1 cctggttgat tttttcgcca cctggtgtgg tccgtgcaaa atcatggcgc cgatgattga
       61 agagctggcc aaagaatatc aggggcaaaa agttaacatt gccaaactgg atattgatca
      121 gtccagagca atcgttcaga aatttaatat tatgtctgtg ccgaccttta aattctttaa
      181 aaagggacag gaggttggtg aacacattgg caccagcggg aaggaagatt ttaaggaaag
      241 aattgatgaa ctattaaaat aagcgccaaa tccaaaattc caaatcctaa ataaattata
      301 atcaccaaaa ttcaaaatca taaactatca agatgaactt acttgcccaa tacaagaaaa
      361 tctttttgaa ttttgaacat ttcccgccgg aggcgggtcc gcctttggcg gagaatttat
      421 aatttatttg gaacttagga ttttgaattt tggattttta agaattatgt caaacaataa
      481 tttaaacaat gtgataatca tcggcggcgg catcgccgcc cacaccgcgg ctatctatac
      541 cgcccgggcg agcttaaagc cgctggttat ttccgcggct gaaccggatc agttgagtct
      601 gaccacgacg gttgaaaact ggcccgggta tgttgacggc gttcaggggc cggagctgat
      661 tgctgatagt aaaaaacaag ctcaaaaatt tggcgcggaa tatattagcg gccaggttaa
      721 ttcagttaaa aaaactgaag aaggttttga cataatcgcc ggagataaaa gttttcagac
      781 aaaaacagtc attatcgcca ccggggcttc cgcccgccgc ttaaatatac ccggcgaaga
      841 tcaatatttt ggccgggggg tcagcacctg cgccgtctgc gacgccgcct tgttcagaga
      901 caaagatgtt gtcgtggtcg gcggcgggga ttcagccatg gaagaaacgt tggctttgta
      961 taaatttgcc cggtcaatta aattgattca ccgccgggac gcatttaaag ccagccagat
     1021 tatgcgggac agagtgctgg ggctaaccga taaggtggaa attttatata gccaccaggc
     1081 gcttgaagtt ttaggcgacg gcaaatttgt gaccggcgtt aaggttaagg atttaaaaaa
     1141 tgacaaggac tatgaaattg ctgtcaatgg aatgtttctg gcgattggcc acactcccaa
     1201 cacaaaattt ttagaaggat tggttgagct ggatgaccag ggctatattg tgacagataa
     1261 acgaagccgg acatccgttg aagggattta cgccgccggc gatgttcagg atccaatttt
     1321 caggcaggcg attacttcgg ccggggcggg atgtcaggcg gcattggagg cggagaggca
     1381 tattgaaaat aggcaatagg caataggcac aaggcacaag gggtggtgtc gcttcgcgac
     1441 attttttctt aaagggaaca ggcaaaaggg tatttaagct tcttaattaa agttaagagc
     1501 atccgaaata tctcatctat taaattatct atttcctggc attttggtat taaaccggtt
     1561 ctcttaattg cctgccccct ggtgcctcgc ttattgcctt gtgcctcttg ccttgtgcct
     1621 tccacttaat caaaacagcc cgtggaaacg ccacagggct gtttttattt gttctgatca
     1681 cgccagctta ttatggcaat ccctgaattt ccagactaaa gaagacatca aagaaagaat
     1741 ctccgctttg gaaacggaca ttgccgcttt gattgtctcc ggtaaagccg gagcagggga
     1801 agttgcttgg caactggact gaactggact gagtcggatc tgatccgggg aagacgccgg
     1861 ttgactgact gaaattaacc caggccggac tgctgataat tgaccagggc tggccgcccg
     1921 gaccggacag ggcgattgtc tggaagggat cagggcagtt gctttcgcca taaacatgga
     1981 ctttgctgat actgctgggt gaagctgaaa ttgggggcat ttccggctcg ggttctggag
     2041 ttggttctgg agttggttct ggggttggct ctggggttgg ctccggttct ggagttggtt
     2101 ctggggttgg agttggcgcc ggggttggag ttggcgttgg ggttggttca ggttgcggag
     2161 ttgggacggt tgttggcggg ccagctccgg gttcagttaa aactcctgat tcatcaggta
     2221 ccggaataat tccgatatta gtgttaattt caggacaaat tcttttgccg ggatcgccgg
     2281 gctctccggc gggatctccc tgaccaccat caccgccctg gccttcagga cagccgttgc
     2341 cgccgttacc gccatcgccg ccgggctttt cttcaactac gcccggctcg cctggcccgg
     2401 ttttaccagt accagccggc ccttcagtag cgtcagcgtc gccgccgttg ccgccattgc
     2461 ctcctggtcc ccgcgggccg cagtggccgc cgtcgccgcc aaagccgccc agggcggtcg
     2521 tgtcgccgcc atcgccgcca aatgaatcaa ctgttcggga agtggcgtcg ccaccggcgc
     2581 cgccagtggc ggttccagtg ccgccctggc cgccatcgca gccgcagccg gtgccactac
     2641 cgcctttgcc ggggctggca attgaccggc cgccttctcc gccgtagagc ggatggatag
     2701 taatattgct ggtgccggca atcgtgccga caacttttaa gccttttttg ttgtcaccgc
     2761 cctggccgcc ggtggcgata gcgtcgccgc ctttggttcc gggacagttg tcttcgccgt
     2821 ctttgccgtg agcggtggct tcaccgcccc ggccgccccg gccgggataa atgttaaacg
     2881 gaccaacgat ttcaaattta ttggaagcgt tcattttaaa gttgcccggt tcgccgccct
     2941 ggccaccggt ggcaatgccg ggatcacact cattatcagg cgtttccgcg tcgccgcccc
     3001 ggccgccata gcccagccag agatcaaagc tgacaacggt aatttcgccg gcaatcaccc
     3061 ggaagcggaa agcgttctcg cccggcttac caacggccgc gcagttttca ccttcgtcag
     3121 catctcccgg ccgtccgtcc ggaccaaaca aagtaaaatt ttcaataata atcttaccgt
     3181 tggctccaaa attaaaattt aaaataattt tatcaacatc tttgggagga attggcacgc
     3241 ccagttcttc gggcggggtc tggccatggc ccatcaccca gcggccgccg attttaacgg
     3301 tgtcattgac cggccagcca aaaaaatctt cggccggcac ctgagcctgg acagggttga
     3361 tcaaaatatt ttttaatttt ttaaccagac tgatctggta agttttggcg gttgccctgg
     3421 gagtcaggac tgtttcattt tcaccaccac cggcgttaga agccgcctgg cccggggcca
     3481 aaggaatatc aataaatggc ccggttctga aaccagctcc gctgtcagtg ccggcgtctt
     3541 gatagatttg atccagttct tctttagtcc tggccagatc agccggactt ttaaccagct
     3601 ggaggctgtt attggtaata atcatggccg aggaggcaaa ttcaatgcct tctgttacca
     3661 ccagattgat cccgtcagcc agtttttccc ctgacaaatc ttcaggctgt tcgcatttta
     3721 attcacctct gaccagcagg gcgccgttga ccgttaaatt taaagggccg ttggcgcatt
     3781 ccaggtcgcc ttcaaccgcg aaattgccgt taactgtcag tttggcgcca tcttttaaaa
     3841 tcattttttc tgaagccgga attaaatggt cgccttccag agtaatatca gtatcaacaa
     3901 ccgtcggccg gttgaaatac cagaaaacac cgccaatgac gacaattaaa ccaacaaccg
     3961 cgccaatgac tatttttgtg gacttggaca taaattaaaa aattaatgat taagaagaaa
     4021 tacgttgggt tattataaca tagaagagaa aaaggaaaca aaaggaatca aggggaagca
     4081 gaagggagca gggggaatga gaaggaatga gaggggcaag ggggataagg gaggattaat
     4141 gtcaaatgac aaagctcaaa tatcaattca aatccaaatg ccaaatgaca atttttagat
     4201 tttgcctgcc tgccgcaggc agggatttta agcttgattt gatctttgga ttttgaaatt
     4261 tgatatttaa aatccttgat ttcccctgct atcccctgat aatccctgat aacccttgtt
     4321 caataaaaaa cggttccagt tttaaactga aaccgcgtgg gtagatggtt cggaagcgaa
     4381 acc
//
LOCUS       DASWHZ010000107         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3196458, whole genome shotgun sequence.
ACCESSION   DASWHZ010000107 DASWHZ010000000
VERSION     DASWHZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196458"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VGA49_02385"
     CDS             <1..503
                     /locus_tag="VGA49_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664646.1"
                     /translation="GQHRVEVAADLINYVEELDETNNFLIIDLELTITEEEKDLYNYK
                     VIKLADDPKVYNVVNGVLHWIPSKEIFLANGYNWGDIIIVPREVLGQYDLGDEVSYYN
                     KLKDGTLIKLEGDIKIYSIDSGFKRYILSPEVFEARGYNWGDVITLSKGEFDSYPEGE
                     GLNFKN"
     gene            525..725
                     /locus_tag="VGA49_02390"
     CDS             525..725
                     /locus_tag="VGA49_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664647.1"
                     /translation="MQSKSTKYKVSARGCTPWRHAVDPPPRPRSAKRGGPADKVQSFG
                     IIKKTVRLDIARRFLVLANFVI"
     gene            735..1457
                     /locus_tag="VGA49_02395"
     CDS             735..1457
                     /locus_tag="VGA49_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9664648.1"
                     /translation="MMTTTIFVLIVAFFASALSAIAGFGGALILIPMVSFFIDFKLAV
                     GVLTFYFLANKIFQIYFYRRSIDWKTAGWVWLGAVPAVIVGALTLVSAPTEILKRMLG
                     ILILFYIINVVYKLTREFKPGKVSTPVIGAAYGFFSGVVGVGDPIKAALLLQIGLVKE
                     KFVATMAIIAILLNLIKAIVYSSFALVSADDVQLIIFLIIVSLLGTYLGKQFLNRLKP
                     EVFKKIVLGLLLIVGLQLIFYP"
     gene            1513..>2112
                     /locus_tag="VGA49_02400"
     CDS             1513..>2112
                     /locus_tag="VGA49_02400"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX9664649.1"
                     /translation="MNDLQGLEIRSALLIFTFLANFVLATIVLFKNYKNKVNIYFSVF
                     VYALAGWAISHAFLILTTDLIRGLFYIKFAYISAWFIAVFFLLFVIYFPYQPISITKL
                     QKSIFAIISMVILLIIIWPNTLLVEVVRENGVKDIIFNKIGQTIFTIYYLLVFALIFY
                     FQILKYVTSRGILKIQVSYVLIGTGIAAIFGLVLSLILPQ"
BASE COUNT          562 a          303 c          481 g          766 t
ORIGIN      
        1 gcggtcaaca tcgggttgaa gtcgcggctg atttaataaa ttatgtcgag gaattggatg
       61 aaacgaacaa ttttttgata attgatttag agctcactat tacggaagaa gaaaaagatc
      121 tttataacta caaagtaata aagttagctg atgatcccaa agtctataat gttgtcaacg
      181 gggttttgca ttggatcccc agtaaggaaa tatttttagc caatggatat aactggggag
      241 atattatcat cgttcctcgg gaagtccttg gtcaatatga tctgggcgat gaagttagtt
      301 attataataa attaaaagac ggaaccctga taaaattaga aggggacatc aagatttata
      361 gtattgatag tggttttaaa aggtatattc taagccctga ggtatttgag gctaggggat
      421 ataactgggg cgatgtcata actttatcta aaggtgaatt tgattcatac ccagaagggg
      481 aagggttaaa ttttaagaat taagaagtaa gttcaaagta caaagtgcaa agtaaaagta
      541 caaagtacaa agtttcagcc aggggctgca cgccatggcg gcatgccgtg gatccgcctc
      601 cccggcctcg ctccgcgaag cggggcgggc cggcggacaa agttcaaagt tttggaatta
      661 ttaagaaaac cgtccggctg gatattgccc ggcggttttt ggttttggct aattttgtta
      721 tataattaca gggtatgatg accactacga tttttgttct gatagtcgct tttttcgcct
      781 cggctttgtc ggccatcgcc ggctttggcg gcgctttgat attgataccg atggtatctt
      841 tttttattga ttttaaactg gccgtcggcg tcttaacttt ttattttctg gctaataaga
      901 tttttcagat ttatttttac agaagaagta ttgactggaa aaccgccggc tgggtctggc
      961 tgggggcggt gccggcggtg attgtcgggg ctttaacttt agtttccgcg cccacggaaa
     1021 ttttaaaaag aatgctgggg attttaattt tgttttatat cattaatgtt gtttataaac
     1081 tcacccgtga gtttaagccg ggtaaagttt caactccggt tattggcgcg gcttatggtt
     1141 tcttttccgg agtggttggg gtcggcgatc cgatcaaggc ggctttgctg ttgcaaattg
     1201 ggctggttaa agaaaagttc gtggcgacca tggccataat tgccatattg ctcaatttaa
     1261 tcaaagcgat agtttattcc agttttgctt tggtgtcggc tgatgatgtc cagttgataa
     1321 tttttttgat catcgtcagt ctgctgggaa cttatttggg aaagcaattt ttgaacaggc
     1381 tcaagccgga ggtttttaag aagatagttt tggggttgtt gttaattgtc gggttgcagt
     1441 tgatttttta tccgtagtgg ggtatatagt atttagaata agatttaata attaactatt
     1501 tgtatgtggt ttttgaatga tttacaaggg ttggaaattc gtagtgcgtt gctgattttt
     1561 acctttctgg cgaattttgt tttagctacg attgttttat ttaagaatta taaaaataaa
     1621 gttaatattt atttcagtgt ttttgtttat gcactagctg gctgggcaat atcacatgct
     1681 tttttaatat tgactacgga tttgattaga ggattatttt atataaagtt tgcttatatt
     1741 tcagcttggt ttattgctgt ttttttctta ctttttgtaa tctattttcc ttatcagccc
     1801 atctcaatta caaaattaca aaaaagtatt tttgcaatta ttagcatggt tattctattg
     1861 attatcattt ggcccaacac cttgttagta gaggtcgtta gagaaaatgg ggttaaggat
     1921 attattttca ataaaattgg ccagacaatt tttacaattt attatttact tgtttttgct
     1981 ttgatatttt attttcaaat tttgaagtat gttactagta gaggtatctt aaaaattcaa
     2041 gtttcttatg ttttgattgg tactggcata gctgcaattt ttggtttggt attaagttta
     2101 attttaccgc ag
//
LOCUS       DASWHZ010000108         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_962822, whole genome shotgun sequence.
ACCESSION   DASWHZ010000108 DASWHZ010000000
VERSION     DASWHZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962822"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VGA49_02405"
     CDS             <1..1609
                     /locus_tag="VGA49_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX9664650.1"
                     /translation="VGRLLASADESSFTEKIIKMRNEFSIAAPLLAQAAGSLAAVDLK
                     AIPEASQSDFNNLKAGLPVVSQVIDRFIAESEALLGILGHYQKKRYLIVFQNNTEIRP
                     TGGFMGSYALVDVKQGKIVNLEIPGGGFYDLQGSLRVKAVSPEPFHLINPVWEPQDAN
                     WFFDFPTSAEKIIWFYYKAGGPTVDGLITLNTFVLKDILAMTGPVDMPQYGRTITADN
                     FIEEIQTVVEFEYDRQLNRPKQIIADLAPVLIERLFEFQESQYLGLTDILAKNLAEKD
                     LLLYFTDDQTEKLVRDLDWAGQVKETGKDYLAVVNTNIAGGKTDGVIRQKISHQAEIM
                     PDGSLIDTVRVARTHLGNKANIFTGVNNVDFIRIYTPLGSTLLSADGFSPPPAEYFED
                     PPEGYYYDQDLSAIESQKLVDPLTQTVITTENNKTVFGNWVQVDVGQTAEAVFKYRLP
                     FKIKFSENKPAGLTDKLKSFLGLTNQEMVYYSLLIQKQPGDWQTDFNSQLTAPADWRL
                     EWSYPESFDQTILNTDKFYGKVFTNKL"
     gene            1627..>3192
                     /locus_tag="VGA49_02410"
     CDS             1627..>3192
                     /locus_tag="VGA49_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664651.1"
                     /translation="MPKDTDNQNDIMDDKSDSGRLKKQAKPGGVRAKQDAGDELIKIY
                     QEENGEEDDDMSKIKPKKRSKKAAAIGTIIVLLLVLLGLSVLSWFLFYKSPKFSGANI
                     TFEITAPEEIKSGQTVEYLIDYGNFESVHFRKAQISVRYPDGFIMDQAEPRPTEADNL
                     WNLGALARGESGQIKISGQLIGDLDSAKILSAALNYLPSNFNSEFEEVASAETKITDS
                     LLTLTLEGPEHALVGEKFDYLIKFKNDSEEELKNVQVRLGYPVNFTAEETVPETDDEL
                     LEEWVFDSLAGGEEVRIKITGSFITAGEDEQKLTARLSLKVEDLSYQQREEFVVTRVI
                     MGDLSLNLIVNGSSTETAANFSSSLNYSINYKNNTDNDLEDIQLTVLISSVKTKGSVS
                     RTGDGIVDWTSLVDANRGILINESIESDRTDILEIRSITWTATQIDNLDKLEKDDEGS
                     INFSVGLKNLNQIEETFGLADLANLELESKVKVVIGKAAGIVSNSEIETSPLIIKMNS
                     DLVFEGEARYHDQS"
BASE COUNT          938 a          642 c          753 g          859 t
ORIGIN      
        1 cgtcggccgg ctgctggctt cagccgacga gtccagtttt actgaaaaga ttattaagat
       61 gagaaatgag ttttcaatcg ccgcgccttt gctggctcag gcggccggta gtctggcggc
      121 agtggacctg aaggctattc ctgaagccag tcagtctgat tttaataatt taaaagccgg
      181 cttgccggtt gtcagccagg tgattgaccg gtttatcgct gaaagcgaag ctttgctggg
      241 tattttgggc cattatcaaa aaaaacgata tttaattgtt tttcaaaata atacggaaat
      301 ccggccgacc ggcggcttta tgggcagtta cgcgctggtt gacgtcaaac agggtaaaat
      361 agttaatctg gaaattcccg gcggcggttt ttacgacctt cagggcagtt tgcgggtcaa
      421 agcggtttct ccggaaccat ttcatttaat taatccagtt tgggagccgc aagacgccaa
      481 ctggtttttt gattttccaa cttcagccga aaaaataatc tggttttact ataaggccgg
      541 cggaccgacc gttgacggcc tgatcacttt gaacaccttt gttttaaaag atattttggc
      601 gatgaccggc ccggttgaca tgcctcaata cggccggacg atcaccgctg ataattttat
      661 tgaggaaatt cagacagtag tagagtttga gtatgaccgc cagcttaacc ggcccaaaca
      721 gattattgcc gacctagctc ctgttttaat tgaacgtttg tttgaatttc aggaaagcca
      781 gtatcttggc ctgactgata tcctggctaa aaatttggcc gagaaagatt tattgcttta
      841 ttttaccgat gaccagactg aaaaactggt tcgggatctg gactgggccg gccaggttaa
      901 agaaaccggc aaagattatc tggcggtggt caacactaat attgccggcg gcaaaaccga
      961 cggggttatc agacagaaga tcagtcatca ggccgaaatc atgccggacg gcagtctgat
     1021 tgacacagtc agggtcgcca gaacccatct cggcaataaa gctaatattt ttaccggcgt
     1081 caacaatgtt gattttatca ggatttacac ccccttgggc agtactttgt tgtcagccga
     1141 cggatttagt ccgccaccgg cggaatattt tgaagacccg cccgaaggtt attattatga
     1201 tcaggatctg tcggcgattg aaagtcaaaa actggttgat ccgctgaccc agacggttat
     1261 tacgactgaa aacaataaaa ctgtttttgg caactgggtc caggtggacg ttggtcagac
     1321 ggccgaagct gtttttaaat atcgcctgcc ttttaaaata aaattttcag aaaataaacc
     1381 agccggtctg acagataagc tgaaaagttt tttgggattg acgaatcagg aaatggttta
     1441 ttactctttg ctgattcaaa aacagcccgg cgactggcaa actgatttta acagccagct
     1501 gacagcgccg gctgactggc gtctggagtg gtcatatcct gaaagttttg atcaaactat
     1561 tttaaatact gataaatttt acggaaaagt ttttacaaat aaactataac ctaaaaccta
     1621 aaaattatgc caaaagatac agacaatcaa aatgatatta tggacgataa atctgacagc
     1681 ggcagattaa aaaaacaagc taaacccggc ggcgtccggg ctaaacaaga cgctggtgac
     1741 gagttaatta aaatttatca ggaagaaaat ggagaggagg atgacgatat gtccaaaatt
     1801 aaacctaaaa aaagaagcaa aaaagccgcg gccattggaa ctattattgt tttactgctg
     1861 gttttgctgg gcttgtcagt tttaagctgg tttttatttt acaaatcgcc caagttcagc
     1921 ggggctaata ttacttttga aataaccgct ccggaagaaa ttaaatcagg ccagaccgtt
     1981 gaatatctga ttgattatgg caattttgaa tctgtccatt ttagaaaagc ccaaatatcg
     2041 gtcaggtatc ctgacggctt tatcatggat caggctgaac cgcggccgac tgaagctgat
     2101 aatctttgga atttgggagc tttggccagg ggcgaatccg gccagattaa aatcagcggc
     2161 cagttgattg gcgatctgga ttcagccaaa attttatcag ccgctctcaa ctatctgccg
     2221 tccaatttta attctgagtt tgaagaagtc gcctcggctg aaactaaaat tactgactct
     2281 ctcctgacct tgactttgga aggcccggaa cacgctctgg tcggtgaaaa atttgattat
     2341 ttgattaaat ttaaaaatga ttcagaagaa gaactcaaaa atgttcaggt ccggctcggt
     2401 tatccggtta attttacggc tgaagaaaca gttccggaga ctgatgatga attgctggaa
     2461 gaatgggttt ttgacagcct ggccggcggt gaagaagtcc ggataaaaat aaccggcagt
     2521 tttattacag ccggcgaaga tgagcaaaaa ctgacggcca ggctcagtct gaaagttgaa
     2581 gatttatcct atcagcagag agaagaattc gtggttaccc gggtgattat gggtgatttg
     2641 tcgctcaatt taattgttaa cggttccagc acagagactg ccgccaattt ttcaagcagt
     2701 ttaaattatt caattaatta taaaaacaac acggataatg atctggaaga cattcagctg
     2761 acggttttaa tcagctcggt taaaaccaaa ggatcagtca gccggaccgg cgacggaatc
     2821 gttgactgga ccagcctggt tgacgccaac cggggaattt taatcaacga atcaatagag
     2881 tccgatcgga ctgatatttt agaaatccgg tccattacct ggaccgcgac ccaaattgat
     2941 aatttggata aacttgaaaa agacgacgaa ggctcaatca atttttcagt tggtttgaaa
     3001 aatttaaatc agattgaaga aactttcggc ctggctgatc tggctaatct ggaactggag
     3061 tcaaaagtca aagttgtcat tggcaaagcg gccgggattg tttccaacag cgaaattgaa
     3121 accagtccgc tgataattaa aatgaattca gatttggttt ttgaaggcga agcccgttac
     3181 cacgatcagt cc
//
LOCUS       DASWHZ010000109         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_220274, whole genome shotgun sequence.
ACCESSION   DASWHZ010000109 DASWHZ010000000
VERSION     DASWHZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220274"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGA49_02415"
     CDS             complement(<1..626)
                     /locus_tag="VGA49_02415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005964472.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9664652.1"
                     /translation="MSKGKITQVIGNVVDVKFEEQTPAIYNALKIKQDKEILTLEVQQ
                     HLGNNIVRTVALGSTDGLSRDVEVKDTGAPISVPVGKETLGRMFNVLGEPIDKGPAVK
                     TKETAPIHKPAPEFKEQSTKTEILETGIKVIDLIAPIVKGGKVGLFGGAGVGKTVIIT
                     ELIRNIATEHGGYSVFAGVGERTREGNDLYHEMKESGVLDKTSLVFGQ"
     gene            complement(812..>1625)
                     /gene="atpG"
                     /locus_tag="VGA49_02420"
     CDS             complement(812..>1625)
                     /gene="atpG"
                     /locus_tag="VGA49_02420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX9664653.1"
                     /translation="QKAVRNVMATRPYANLAWDFLGELVKRTDSQKHYLLERRPVKKV
                     GLILISSNRGLCGAFNTQIAQKALKSIQLHEGQVLETEFITFGKRGRDILLKSKVNIT
                     ADFNKPDVLQTIIEIRPLTHLVIDEYKKGTYDKVMLVYTDFVSSLKQVPRVKHLLPIE
                     PEQDEYIGHVAGDRQTEFAEEVSYEYEFLFEPSIDDVLKSLLPRLVEMQIYQAVLESD
                     ASEHSTRMLTMKNATDAASEMIDELTLVYNQARQAGITQEIAEISAGQAAIN"
BASE COUNT          428 a          386 c          326 g          485 t
ORIGIN      
        1 atttgtccaa aaaccagaga agttttatcc aaaacgcccg attctttcat ttcatgatac
       61 aagtcattgc cttcacgggt tctttccccc accccggcaa aaactgaata accgccgtgt
      121 tcagtggcaa tatttctgat cagctcggta ataataacgg ttttaccgac cccggcgccg
      181 ccaaacaaac caactttgcc gcctttgaca atcggggcga tcagatcaat caccttaatg
      241 ccggtttcca aaatctcggt ttttgtggat tgctctttaa attcaggcgc tggtttatgg
      301 attggcgctg tttctttggt tttgacagcc gggcctttgt caatcggctc gcccaaaaca
      361 ttaaacattc ggccaagtgt ttctttacca accggcacgg aaattggcgc gccggtgtct
      421 ttgacctcta catcccgcga taagccgtca gttgacccca gggcaaccgt ccggacgata
      481 ttattgccca aatgctgctg aacttccagt gtcaaaattt ctttgtcctg ttttattttc
      541 agggcattat aaattgcggg agtttgttcc tcaaatttta catcaacgac attgccgatg
      601 acctgagtga ttttaccttt agacataaaa ataatattaa ttattaggca ctaggcaaat
      661 ggcaataggc attggaaata attttattaa ggttgatgag ggttgatgaa ggttgataag
      721 ggttgatgag gggcttaatc cctcattaac cataatcttc cctaataaac cttcataatc
      781 cctaatacct tgcctggtgc ctgttgccta attagtttat tgcggcttgg ccggcggaaa
      841 tttcagcaat ttcctgggta atgccggcct gacgggcctg attataaact aaggttaatt
      901 catcaatcat ctcgctggcg gcgtccgtgg cgttcttcat ggtcagcatc cgggtagaat
      961 gctcgctggc gtctgactcc agtaccgcct gataaatctg catttccacc agccgcggca
     1021 ataaactttt caaaacatcg tcaattgaag gctcaaataa aaactcatat tcataactga
     1081 cttcttcggc gaattctgtt tgacggtcac cggcgacatg gccgatgtat tcatcctgtt
     1141 caggctcaat cggcagtaaa tgtttcaccc ggggaacctg cttcagggac gaaacaaaat
     1201 cagtgtaaac cagcatcact ttgtcataag tccccttttt atattcatca atcactaaat
     1261 gagtcagtgg tctgatctca ataatagtct gtaaaacatc cggtttatta aaatcagccg
     1321 taatgttaac ttttgatttt aataaaatat cccggccccg tttgccaaaa gttataaatt
     1381 cagtttccaa cacctggcct tcatgaagct gaattgactt taaagctttt tgggcaatct
     1441 gagtgttaaa agcgccgcac aaaccccggt tggaactgat taagattaaa ccgacttttt
     1501 ttaccggccg tctttccagc aaataatgtt tctgggaatc agttctttta accagttccc
     1561 ccaaaaaatc ccaggctaaa ttggcgtaag gccgggtggc cataacattt ctgaccgctt
     1621 tttgc
//
LOCUS       DASWHZ010000110         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_598778, whole genome shotgun sequence.
ACCESSION   DASWHZ010000110 DASWHZ010000000
VERSION     DASWHZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598778"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /gene="uvrB"
                     /locus_tag="VGA49_02425"
     CDS             <1..1916
                     /gene="uvrB"
                     /locus_tag="VGA49_02425"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9664654.1"
                     /translation="LKSQYQPTGDQPTAIKQLVDGFDKNKQQTLLGVTGSGKTFTMAN
                     VIQQVQRPTLVISHNKTLAAQLYNEFKEFFPENKVCYFISYYDYYQPESYLPVTDTYI
                     EKEVLINERLEEFRLDAISSLVSRNDVIVVASVSCIYGLGDPADFKEGAKTVATGQEI
                     NHQDFIRELVNLQFERNDTELKSGNFRVRGDVIDLIQGFGKFIYRIEFFGDQIEKMTE
                     RETVSGKLIQEIDQLVIFPANPYIINENNKTRALKAIRRELKASLPALEPLEAHRLEK
                     RTNYDLEMIEQIGHCKGIENYSRHFDGRLPGTPPRCLLDFFPKDFLMIIDESHATIPQ
                     IRGMYEGDRSRKNNLIENGFRLPSAYDNRPLKFKEFEKYLDHLIYTSATPGDYELKNS
                     GRVIEQIIRPTGLVDPEISLRPIEGQIDDLIKEINQTVDNDNRVLVTTLTKKSAEDLS
                     EYLANQGIKVRYLHSEIDTIERTEIIRDLRQGKFDCLVGINLLREGLDIPEVELVAIL
                     AADKEGFLRNTRSLIQTSGRAARNVSGRVVMYADKKTGSIRETLRETARRRQKQLEYN
                     KERNIRPQSIEKAIQGRLSVEIAKSKKKFKDKDDLLDYAAELEFEMKKAAEELDFEKA
                     IDLRDEIHELMTG"
     gene            1920..>2330
                     /locus_tag="VGA49_02430"
     CDS             1920..>2330
                     /locus_tag="VGA49_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9664655.1"
                     /translation="MAKEYLKIKGAREHNLKNIDINIPLDSFTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMNKPDVDSISGLSPAISIEQKSVSKNPRSTVGT
                     ITEIYDYLRLLYARIGQPHCPRCRQPISSQSAESI"
BASE COUNT          716 a          457 c          541 g          616 t
ORIGIN      
        1 atttaaaaag ccaatatcag ccaacaggcg accagccaac agccattaaa cagctggttg
       61 atggatttga taaaaataaa cagcagactt tactgggagt gaccggatca ggcaaaactt
      121 ttacgatggc taatgttatc cagcaagtac aaaggccgac tttagtgatt tctcataata
      181 aaactttggc cgcccagctt tacaatgaat ttaaagaatt ttttcctgaa aacaaggttt
      241 gctattttat ttcttattat gattattacc agccggaaag ctatctgccg gtgactgata
      301 cttatattga aaaggaggtt ttgattaatg aacggttgga agagttcaga ctggacgcga
      361 tttcttcttt agtcagccgg aacgatgtta ttgtggtggc ttcggtttcc tgcatttatg
      421 gtttgggcga tccggccgat tttaaagagg gcgctaaaac agtcgctaca gggcaggaaa
      481 ttaaccacca ggattttatc cgggaactgg ttaatctcca gtttgaaaga aatgacactg
      541 aattgaaaag cggcaatttc cgggttagag gtgacgtgat tgatctgatt cagggttttg
      601 gcaagtttat atacagaatt gaattttttg gcgatcaaat tgagaaaatg actgaaaggg
      661 aaacggtcag cggcaagtta atccaggaaa ttgaccagct ggtaattttt ccggccaatc
      721 cctatattat taatgaaaac aataaaaccc gggctttgaa agctattcgc cgggagttaa
      781 aggcgagttt accggcactg gaaccattag aggctcatcg cctggaaaaa agaaccaatt
      841 atgatctgga aatgattgag caaatcggcc attgcaaggg gattgagaat tattccagac
      901 attttgacgg tcgtttgccc ggcacgcccc cccgctgtct gcttgatttt ttccccaaag
      961 attttttaat gattattgat gaaagccacg cgacaattcc gcagattagg ggaatgtatg
     1021 aaggcgaccg gtccagaaaa aataatttga ttgaaaacgg ttttcgactg cccagcgcct
     1081 atgataaccg gccgctcaaa tttaaagaat ttgaaaaata tctggaccac cttatttata
     1141 ccagcgccac gcccggcgac tatgaactga aaaacagcgg ccgggtaatt gaacaaatta
     1201 ttcgcccgac cggcctggtt gatccggaaa ttagtttgcg gccaattgag ggccagattg
     1261 atgatttaat taaagaaatc aatcagacag tggacaatga taaccgggtt ttagtgacaa
     1321 ccttaactaa aaaatcagcc gaagatctgt ctgaatatct ggctaatcag gggattaaag
     1381 tccgttacct tcatagtgaa attgatacta ttgaacgaac agaaataatt cgtgatttac
     1441 ggcagggcaa atttgattgt ttagtcggca ttaatttatt aagagaaggt ctggatattc
     1501 cggaagtgga gctggtggct attctggccg ccgacaaaga gggttttttg cgtaatacaa
     1561 gaagtttaat tcagacctcc ggccgggcgg cccgaaatgt cagcggccgg gtggtaatgt
     1621 acgctgataa aaagaccggc tcaatcaggg aaactttgcg ggaaaccgcc cgccggcgtc
     1681 aaaaacagct ggaatataat aaagagagaa atattaggcc gcaatcaatt gaaaaagcca
     1741 tccaggggcg gttaagcgtt gaaattgcca aatccaaaaa gaaatttaaa gataaagatg
     1801 atttgctgga ttacgccgct gaactggaat ttgaaatgaa aaaagcggcg gaagaacttg
     1861 attttgaaaa agcgattgat ttgcgcgacg aaatccatga attaatgacc ggttaaatta
     1921 tggccaaaga atatctgaaa attaaaggag cccgcgaaca taatttgaag aatattgata
     1981 tcaatattcc tttggatagt tttacggtga ttaccggttt atccggttca ggtaaatcat
     2041 ctctggcttt tgacacgatt tacgccgaag gccagcgccg ctacgtggaa agcctgtccg
     2101 cttacgccag gcagttttta ggtttaatga ataagcctga tgtcgactcc atcagcggtt
     2161 taagcccggc tatttctatt gaacaaaaaa gtgtttcaaa aaacccccgg tcaaccgtcg
     2221 gcaccattac cgagatttat gactatttaa gattgctcta cgcccgaatc ggccagcccc
     2281 attgtccgcg ctgtcgccag ccgatttcat cccagtcagc tgaaagcatt
//
LOCUS       DASWHZ010000111         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2820915, whole genome shotgun sequence.
ACCESSION   DASWHZ010000111 DASWHZ010000000
VERSION     DASWHZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820915"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VGA49_02435"
     CDS             <1..260
                     /locus_tag="VGA49_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9664656.1"
                     /translation="AMLFIFDKKDYYSFWMKEMEFPIDIIWIDDQTVVGIEKNIQPPA
                     AGRLFVYRPEQKVNLVLEIGAGLSDQRNIKIGDLVDISYSD"
     gene            294..602
                     /gene="rplU"
                     /locus_tag="VGA49_02440"
     CDS             294..602
                     /gene="rplU"
                     /locus_tag="VGA49_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9664657.1"
                     /translation="MKIAVIKTGGKQYKVKANDRLKIEKIKGDEGQTVEFDQVLLVAD
                     GQKVEIGQPFLAGKKITARILKQSRSKKVTVLKYKPKTRYRRKKGHRQAFTQIEIEAI
                     "
     gene            complement(604..1203)
                     /gene="recR"
                     /locus_tag="VGA49_02445"
     CDS             complement(604..1203)
                     /gene="recR"
                     /locus_tag="VGA49_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9664658.1"
                     /translation="MQKFPEPVKNLIEQFNRLPGIGHKTSQKFVFYLLKQPKEVLTGF
                     AGALLNLNKLIEKCAVCGNIATTSPCSICDNQQRNQNIICVVAETYDLISIENTGDYT
                     GLYHVLGGAINTVEGVSPDQLNIKSLLTRINQKKPEEIILAFSPDLEGESTVLYLKNL
                     LSSYQLKLTRLAKGLPMGSVLEYADEVTLSNALKGRTMV"
     gene            complement(1278..1571)
                     /locus_tag="VGA49_02450"
     CDS             complement(1278..1571)
                     /locus_tag="VGA49_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9664659.1"
                     /translation="MFNKLKQIKDLRSKAKQMQSTLAEESVEASAAWGKVKLTMNGNQ
                     EITSIDIDPELLSPDKKAELEKAVQEAVADAIKKVQRVMAKKMQDMGGLPEFK"
     gene            complement(1680..3047)
                     /gene="dnaB"
                     /locus_tag="VGA49_02455"
     CDS             complement(1680..3047)
                     /gene="dnaB"
                     /locus_tag="VGA49_02455"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9664660.1"
                     /translation="MPDEIIKIPPQNIEAEQSFLGSLLIDKDAVIKVADSVRPEDFYK
                     NVHQMIYEAMLDLYVKHEPIDLLSLSNRLEEKDQLEKIGGRSYLTSLANLVPTASNVD
                     HYGGIVQKKATLRRLLRAASEITKLGYDEVEDVDIVLDQSEKSLFAVSQKYLRNIFVP
                     VKSVLADAFDRIDELHREKGKLRGIPTGYQALDNLLGGLQKSDLVIIAARPSIGKTSL
                     ALDFARTIATKNKIPVGIFSLEMSKEQLVDRLICAEANVDLWRMRTGKLSDREDGGDF
                     PRIGHAMGTLSEAPIYIDDSPTATVMNIKTKARRLQAENGLGLILIDYLQLMEGRGNT
                     DNRVLEISEITRSLKGIARELNIPVIALSQLNRSVESRTPPIPKLADLRESGSIEQDA
                     DVVMFIYREDYYKRGPEKNHIADIFIAKHRNGPTGNIQLYFDENIVSFKNLEKKIKEE
                     DIPGF"
BASE COUNT          929 a          702 c          796 g          999 t
ORIGIN      
        1 aggcgatgct gtttattttt gataaaaaag attattattc tttctggatg aaagaaatgg
       61 agtttccaat tgacattatc tggattgatg atcagacggt tgttggcatt gaaaaaaata
      121 ttcagccacc ggcggccggc cggctgtttg tttaccggcc tgaacaaaaa gtgaatttag
      181 tgctggaaat cggggccggg ctgagtgacc agcgcaatat taaaattggc gatttagttg
      241 atattagcta cagtgattga cagtttttaa atattataat aaactgtttg aatatgaaaa
      301 tagcggttat taaaaccggc ggcaagcaat acaaggtcaa agccaacgac cggttaaaaa
      361 ttgaaaagat caaaggcgac gaaggccaga ctgttgagtt tgaccaggtt ttgctggtgg
      421 ctgacggcca gaaagttgaa attggccagc cgtttttagc cggtaaaaaa atcacagcca
      481 ggattttgaa gcaaagccgg tctaaaaagg tgaccgttct taaatataag ccaaaaacac
      541 gttaccgaag aaaaaaaggc catcgtcagg cctttactca aattgaaatt gaagctattt
      601 aaattaaacc attgtccggc ctttgagggc gttggacaag gtgacttcat cagcgtattc
      661 cagaacagag cccattggca ggcctttggc cagccgggtt aacttcagct ggtatgacga
      721 caagaggttt ttaagataga ggacggttga ttcaccttcc agatcgggac taaaagccag
      781 aataatttct tccggctttt tttgattgat tctggtcagg agagatttga tattaagctg
      841 gtcagggctg acgccttcga ctgtattgat cgcgcctccc agcacatgat agagaccggt
      901 gtagtcgccg gtattttcaa ttgaaattaa atcataggtt tcagccacaa cacagattat
      961 attttggttt ctttgctggt tatcgcaaat tgaacaggga ctggtagtgg ctatattgcc
     1021 gcagacagcg catttttcaa tcaatttgtt aagattcagc aaagccccgg caaaacctgt
     1081 cagtacttct ttgggttgtt tcaacagata aaaaacaaac ttttgggaag ttttgtggcc
     1141 aattccaggc agacgattga actgttcaat cagattttta actggttcgg gaaatttttg
     1201 cataaaataa gaggggagat aaggggggat aaggggggat gagggtagat gagggtagat
     1261 gaggagatgg gaattgttta tttaaattct ggcaggccgc ccatgtcctg catttttttg
     1321 gccatcaccc gctgaacctt tttaatggcg tcagccacgg cttcctgaac agctttttcc
     1381 agttcagctt ttttgtccgg actaagcagt tcaggatcaa tatcaattga ggtgatttcc
     1441 tgattaccgt tcatggttaa tttgacttta ccccaggcgg cgctggcttc aacagattct
     1501 tcagccaggg tgctttgcat ttgtttggct tttgaccgaa gatctttgat ttgtttgagc
     1561 ttgttgaaca tatgtttagg gtatagggta taggggatag ggtatagggt atagtcagtt
     1621 gaaaatattc cattcactat tgcctattaa tctatcccct ataccctata ccctataccc
     1681 tagaacccag gtatatcttc ctctttaatt tttttttcta aatttttaaa gctgacgata
     1741 ttttcgtcaa agtaaagctg aatattgccg gtcgggccgt tgcggtgctt ggctataaaa
     1801 atatcggcaa tatggttttt ttccggacca cgcttgtaat aatcttcccg ataaataaac
     1861 atcaccacgt cagcgtcctg ttcaattgat ccggattccc ggaggtcagc cagtttgggg
     1921 attggcggcg tccggctttc aactgaccgg ttgagctggg acaaagcgat caccgggata
     1981 ttaagttctc tggcaatccc tttgagtgac cgggtaattt ctgatatttc caggacccgg
     2041 ttgtcagtat tgccccggcc ttccattaac tgcagataat caattaaaat caggcccagg
     2101 ccgttttccg cctgcagccg ccgggctttg gttttaatgt tcatgactgt cgctgttggc
     2161 gaatcatcaa tataaatcgg ggcttctgac agtgtcccca tggcgtgacc gatccgggga
     2221 aaatcgccgc catcttcccg gtcagacagc ttgccggttc gcatccgcca caaatcaaca
     2281 ttggcttcag cacaaattaa gcggtcaacc agctgttctt tggacatttc cagactgaaa
     2341 atgccaaccg ggattttatt tttagtggca atcgtccggg caaaatccag agccagtgaa
     2401 gtttttccga ttgacggccg ggcggcgata atcaccaaat ctgatttttg gagaccgccc
     2461 agaagattat ccagagcctg gtagccggtt ggaataccgc gaagtttgcc tttttcccgg
     2521 tggagttcgt caatccggtc aaaagcgtcg gccaaaactg atttgaccgg cacgaaaata
     2581 ttccttaaat atttttgtga gacggcgaac agactttttt cggactgatc cagaacgatg
     2641 tcaacgtctt ccacctcgtc atagcctagt ttggtgattt ccgaagccgc ccgcagcagg
     2701 cggcgaagcg tggctttttt ttggacaatg ccgccgtaat ggtcaacatt gctggcggtc
     2761 ggcaccagat tggccagcga ggttaaatag ctccggccac caattttttc cagttggtct
     2821 ttttcctcca gccggttgct taagcttaac aaatcaattg gctcgtgttt gacgtataaa
     2881 tcaagcatcg cctcatagat catctggtgg acatttttat aaaaatcttc cggccggact
     2941 gaatcagcca ccttaatcac ggcgtctttg tcaatcaaaa gactgcccag aaatgactgt
     3001 tcggcttcaa tattttgggg agggattttg attatttcat ctggcatttt ttaggggata
     3061 gggggtggat tactaagttt tgcggtgctc atattttact aaattttaat tcctaaatca
     3121 agaagataag gggaaaacag aaggaatcaa agggaatcag gaggaaacaa ggggaagaaa
     3181 ggataaaaat ttcaaaatcc agctattaaa tccaaatatc aaagctcaaa tttcaattga
     3241 aatttaaagc ccaaatgtca aaaaaacctt tgaaatttag attttggatt ttgggcttga
     3301 tttgagcttt ggactttgaa ttttgatatt ttaaccctca tctccgctca tctcccctca
     3361 tctcccttta tcccctttaa aaaaaagacc tcaaatgagg tctggaactg cttaacggag
     3421 ctggag
//
LOCUS       DASWHZ010000112         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2467416, whole genome shotgun sequence.
ACCESSION   DASWHZ010000112 DASWHZ010000000
VERSION     DASWHZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467416"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1321)
                     /locus_tag="VGA49_02460"
     CDS             complement(50..1321)
                     /locus_tag="VGA49_02460"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEX9664661.1"
                     /translation="MSILHDKLGQKLPGLREEIKTLVKGNADKVISQVTVSQIAGGLR
                     GVNALICDTSYVDPEKGLIIRKAPIEELADKLPEEIFYLLLLGELPAEDELKTLQTEL
                     KEKSKVPGYVWDVLRAMPADSHPMVMLDTAILVMEKESEFKKQYDQGLAKGDYWRPMI
                     DDCLTLIARVPEIAAGVYRIGYDKGDLIPSNPGLDLGANYAAMLGIDDPTGNLKKLMR
                     LYLTLHCDHESGNVSAFTTHVVASALSDIYYAVSAGLNGLAGPLHGLANQECLNWILR
                     IMEKYNGLPTKEQITEFTHETLNAGQVIPGYGHAVLRKTDPRFTAFVNFGKQACPDDQ
                     IFQTVVAVFETMPEVLGAIEKIKNPWPNVDAASGSLLYHFGLKEFAYYTVPFAVSRIL
                     GISAQTILSRALGAPIVRPKSISTEMLKTMV"
     gene            complement(1547..1762)
                     /locus_tag="VGA49_02465"
     CDS             complement(1547..1762)
                     /locus_tag="VGA49_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX9664662.1"
                     /translation="MRQVINLSLPPEMDKLVRQAVKKGHYASLSEFFRVLLRKWLKDE
                     LYQDVLESEKEFARGQGKKLKSLKELR"
     gene            complement(1827..>2063)
                     /locus_tag="VGA49_02470"
     CDS             complement(1827..>2063)
                     /locus_tag="VGA49_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX9664663.1"
                     /translation="DQKIFFNSEMQSQQIKKFCQIDSACQKMLKDAVEKMRLSARAYF
                     RILKLSRTIADLAGNGQIKVEHLAEALQYRPRVD"
BASE COUNT          550 a          453 c          460 g          600 t
ORIGIN      
        1 ccccccgtca gtcgcgactg acggggacgg gatccaagat tgctgaagtt taaaccattg
       61 ttttcagcat ttcagtagag attgatttgg gacggacaat tggcgctcct agcgcccggc
      121 ttaaaatagt ctgggcggaa attcccagga tccgggaaac ggcaaacggc acggtgtagt
      181 aagcaaattc tttcagacca aaatggtaga gcaaagaccc cgaggcggcg tcaacattgg
      241 gccaggggtt tttaattttt tcaatcgctc ccaggacttc cggcatggtt tcaaaaaccg
      301 ccaccactgt ctgaaaaatc tggtcgtccg ggcaggcttg tttgccaaag ttcacaaaag
      361 ccgtgaatct gggatcggtt tttcttaaaa ccgcgtgacc atagccggga ataacctggc
      421 cggcattgag agtttcatga gtaaattccg tgatttgttc tttggtcggc aggccgttgt
      481 atttttccat gattcgtaaa atccagttga ggcattcctg gttggccaga ccgtggagcg
      541 ggccggccag accgttcagc ccggccgaaa cggcgtagta aatatcagac aaagcggaag
      601 caacaacgtg agtggtaaaa gcgctgacat tgccgctttc atgatcgcaa tgcaaagtca
      661 gatagagccg cattaatttt ttcaggttgc cggtcgggtc atcaatgccg agcatggcgg
      721 cgtaattggc gcccaggtcc aggccgggat tggacggaat taaatcgcct ttgtcatacc
      781 ctatccggta aactccagcg gcaatttccg gcacccgggc gatcaaagtc aggcaatcat
      841 caatcatcgg ccgccagtag tcgcctttgg ctaagccctg gtcgtattgt tttttaaatt
      901 ctgactcttt ttccatgacc agaatggctg tgtccagcat aaccattgga tgggagtcag
      961 ccggcatcgc ccggagcaca tcccagacat aaccagggac ttttgatttt tcttttaatt
     1021 cagtttgcaa agttttcagt tcgtcttcag ccggcagttc gcccaacagc aataagtaaa
     1081 aaatttcttc aggcagttta tcggccagtt cttcaatcgg cgcttttctg ataattaaac
     1141 ccttttcagg atcaacataa gaggtgtcgc agatcagagc gttgacgccg cggaggccgc
     1201 cggcgatctg gctgacagtt acctgggaaa taactttatc agcgttgcct ttgaccagag
     1261 ttttaatttc ttcccttaag ccgggcagtt tttggccgag tttatcgtgg aggatggaca
     1321 ttgatgtggg attaatggtt aatgaatttt attctagcaa attattattt taattggaag
     1381 tgcttattta taaatattat gcgttccaat ggtatggaaa agaatttttt tatctgaaac
     1441 aaatgaaaat ataattctat gatcatgtcc aacagaaaag gcccagtaat tttttatatt
     1501 tcttcaaaaa ttttggtgaa taaaaaattt taaccataga aaggcattat ctcagctctt
     1561 tgagcgactt caattttttc ccctggcctc tggcaaattc cttttcactt tccagaacat
     1621 cctgataaag ttcgtctttc agccattttc ttaacagaac tctgaaaaat tcactaaggc
     1681 tggcatagtg gccttttttg actgcctgcc tgaccagctt gtccatttca gggggtaatg
     1741 acagatttat aacctgacgc ataaaattta aattaattgt aatacattgt attacaatat
     1801 taacatttaa aaataaattt tacaaactag tccaccctcg gccgatactg cagggcttcg
     1861 gctaaatgct caactttaat ttgcccattg ccggccagat cagcaatggt tcttgataat
     1921 ttcaaaatcc tgaaataggc ccgggcggac aaacgcattt tttcaacggc gtctttcagc
     1981 attttttgac aggctgaatc aatctgacag aattttttaa tctgctggga ttgcatttca
     2041 gagttaaaaa aaattttttg atc
//
LOCUS       DASWHZ010000113         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1723970, whole genome shotgun sequence.
ACCESSION   DASWHZ010000113 DASWHZ010000000
VERSION     DASWHZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723970"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..>1906
                     /locus_tag="VGA49_02475"
     CDS             519..>1906
                     /locus_tag="VGA49_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664664.1"
                     /translation="MTEFLVTVASYNQFSTSQAVSNGWVIVRDVANSFFVVVLLVIAF
                     STMLGYQKYGYKALLPKLLIYAILINFSKTIAGLFIDFSQVVMITFVNGFSQLAGGNI
                     IQALQIDQMLNLELGDKPGAIGGTSVLIAVLLSLIMLVIALVLVVIFTVILLFRIVML
                     WLLVVLAPLAFILPLFPGAQKFGNQWWEEFGKYLIVGPLLAFFLWLSFVTIGDGNAIN
                     QLTADPSKISVAGLQGAVSTFSAFGSYIIGVCMLVGGLVLTGKLGVAGGSMAGAAANK
                     IMETGKRAAIAPLKAAGGALTTAEGYIYGKTGFSGFNPMRIGRGIKSAYESTRRRWES
                     EGASIASTRGKELEGEGKVLRGMLRTGFGAPEDFVQNYFSGRGVKKLLYGLKGALPGT
                     KNVAEVRAEAKEKSEGYEAEATLAKNAIGDKEMSYGKRKEAWEGYDGEEAKLDKSISD
                     EEKNQQAIRDRY"
BASE COUNT          512 a          332 c          475 g          587 t
ORIGIN      
        1 acgccggctt cctttccgaa aggagccacc aggatggagg ccataaaggc tgtcggcgcg
       61 aaaccgataa aaaagccaac ggcgcctgcc agcaggccca cgggaagccc tatcccgtag
      121 gcaatgaaaa aagaaaccgc cttgcccaga agggtgatag gatcgttttg caagacaaac
      181 ctcctttatg tctgttttta tccaaaataa gtatagtatg ttttattaat tttgtcaacc
      241 ttatttttat taacagtttt cagctttgct gaataagaca atttatgtta tatttagata
      301 aataaaaaac aaataaatta atgaaatttc cgcggtccaa aagagctaag tatttctatt
      361 tattgatctt attcttagtt attggactgg ggatttttag ttttgccaaa gtttcttatg
      421 ctgcgtctgc tgcgtcttgg tgggataata tagcctgggc tttagcttgg cttgttaatt
      481 ctgtattagt taatttagtg ggctccctgt tggttttaat gactgaattt ttagtgacgg
      541 ttgccagcta taatcaattt agcacttcac aggcagtaag taacggctgg gtgattgtca
      601 gggatgtcgc caacagtttt tttgtggtgg ttttgctggt gattgctttt agcacaatgc
      661 ttggctacca aaagtacggc tataaagcat tattgcctaa gttgctcatt tacgctattt
      721 taatcaattt tagcaaaaca atcgctggtt tgtttattga tttttcacaa gtggtaatga
      781 ttacttttgt caatggtttt tcccagctgg ccggcggcaa tatcattcag gctttgcaga
      841 ttgatcaaat gcttaacttg gaattgggag ataagccagg cgctattgga ggcacctctg
      901 ttttgatagc ggttctttta tcattaataa tgttagtcat tgctctggtt ctggttgtca
      961 tcttcaccgt tatattgctt tttagaatag ttatgctctg gcttttagtc gttctcgctc
     1021 cactggcttt tattttgccc ttgtttcccg gagcccagaa gtttggcaat caatggtggg
     1081 aagagtttgg aaaatatttg attgtcggac cattgctggc tttcttttta tggctgtctt
     1141 ttgtgacgat cggcgatggc aacgcaatta accaattaac agcggatcct tcaaaaatca
     1201 gtgtcgctgg tctgcagggc gctgtttcta ctttttcagc ttttggcagt tatattatcg
     1261 gtgtttgcat gttggttggt ggtttagtgt taactggtaa attaggagta gccggaggaa
     1321 gcatggccgg agcggccgcc aataaaataa tggaaaccgg taaacgggcc gcgattgctc
     1381 ccttaaaagc ggccggcggg gctttgacta cggctgaagg gtatatttat ggtaaaaccg
     1441 gttttagcgg attcaatccc atgagaattg gaagagggat taaatccgct tatgagagca
     1501 cccgtcgcag atgggagtca gaaggcgcct caatcgcgtc aaccagaggg aaggaacttg
     1561 agggtgaagg taaggttttg agaggcatgc taaggacagg ttttggagcg ccggaagatt
     1621 ttgtacaaaa ttattttagc ggtcgcggcg ttaaaaaatt attatatggt cttaaaggag
     1681 ctctccccgg tacgaaaaac gttgcggaag tcagagctga agctaaagaa aaatctgaag
     1741 gatatgaagc tgaagcgact ttagccaaaa acgccattgg tgataaagaa atgtcgtatg
     1801 gaaaaaggaa agaggcttgg gagggttatg acggtgagga agctaagctg gataagtcaa
     1861 taagtgatga agaaaaaaat cagcaggcta tacgggatag atatca
//
LOCUS       DASWHZ010000114         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_604214, whole genome shotgun sequence.
ACCESSION   DASWHZ010000114 DASWHZ010000000
VERSION     DASWHZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604214"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGA49_02480"
     CDS             complement(<1..941)
                     /locus_tag="VGA49_02480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9664665.1"
                     /translation="MKIAHLVSTFPPYTGGMGNVAYHQALELSKLGHQVEVFTLTNKS
                     KFEQFKVNQLKPWLKYGNAGLIPQIFWKLKKFDIVHLHYPFFGGAEIVYFWKLFNRKK
                     KLILTYHMDVVGQGALRYFFKFHNRFILPGIIKRADQVLVSSTDYLNNSNIKANYNKN
                     HEKFTEIPLGVSESFQPEAKDEELLEEYNLSHDDIILLFVGGLDKAHYFKGVELLIKT
                     FAGLSKKINNLKLLIVGGGGMKPDYQRLTTSLNIADRVVFTGRVSDELLPKVYNLSDI
                     FVLPSTDRSEAFGLVILEAMACGKPVVASRLPGVRQVV"
     gene            complement(957..2006)
                     /locus_tag="VGA49_02485"
     CDS             complement(957..2006)
                     /locus_tag="VGA49_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9664666.1"
                     /translation="MVAVIIVNYNGLAYLPDCLTSLKKQTFDHNQFEVFIVDNNSGDH
                     SVDYIKTEFNEFNLFELKENLGFAGGNNYAIRKAMAEGCDYFVLLNQDTIVEPDWLKN
                     LLEAAEADDQIGLVQSRLMLWPQTDKINSIGNVINYLGFGYTRGHQEPESSFDYPRQV
                     HQINYASGAAMLIKRPVIEAIGMFDDDLFMYHEDLDLSWKARLLGYQVVLAAQSVVYH
                     KYQFSRSIAKYYYMERNRFMVIFQNYKWPTIVLISPALILMETGLFFYSIYSGWWTEK
                     VKVYRYLLNFKNLKNISAKRRIIQRKRKLSDKDVVTAFSGKVEFQDINNPVLTYIANP
                     IFNLYWQVVKKVIFW"
     gene            complement(2067..2411)
                     /locus_tag="VGA49_02490"
     CDS             complement(2067..2411)
                     /locus_tag="VGA49_02490"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX9664667.1"
                     /translation="MIFQVLTVVFVLYVFYKIWVRYKDKTINRREFIFWVLVWLIVGV
                     IVLFPELTTYLARLVGIGRGVDLIIYISIAILFYMVFKIFLRIDKIDQEITALTRKTA
                     IDDKLKKSDQSD"
     gene            complement(2461..>2967)
                     /locus_tag="VGA49_02495"
     CDS             complement(2461..>2967)
                     /locus_tag="VGA49_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9664668.1"
                     /translation="GQGAALKTGIDYALNQGAEVIVHFDADGQQEPAEIRPMVSHLLN
                     NNFDIVIGSRFLDKKPQNIPLIRKTGLKLAILVEWFHSGLKLSDAHNGFRAMTRQAAQ
                     KIQLTFDGDSHASEIIGELARNKLNYAEFPVTVKYTKDSLNKDYHKSDPVKALIILKD
                     IFFKKLFS"
BASE COUNT          950 a          643 c          509 g          865 t
ORIGIN      
        1 acgacctgtc tgactcccgg cagcctggag gccaccaccg gcttgccgca ggccatggct
       61 tccaaaataa ccaggccaaa agcttctgac cggtctgttg atggtaatac aaaaatatca
      121 gacaaattat aaactttagg caaaagctca tcgctaaccc ggccagtgaa aacaacccgg
      181 tcagcaatat ttaaacttgt ggttaacctc tgataatccg gcttcattcc gccgccgcca
      241 acgatcaata attttaaatt attaatcttt tttgacaagc cggcaaaagt cttaatcagc
      301 aattcaaccc ccttgaaata gtgagctttg tccagcccgc cgacaaaaag cagaataata
      361 tcatcatgac ttaaattata ttcctctaac aactcttcat cttttgcttc aggttgaaat
      421 gattcgctca ctcccagcgg aatttcagta aacttttcgt gatttttatt ataattagct
      481 ttaatgttag aattattcaa ataatcagtt gacgaaacta aaacctggtc agcccttttt
      541 ataattcctg gcaaaataaa tctattgtga aatttaaaaa aatatctcaa agctccctgc
      601 ccaaccacgt ccatatgata ggtcaggatt aatttttttt tacgattaaa taatttccag
      661 aaataaacta tttccgcccc gccaaaaaaa ggataatgca ggtggactat gtcaaatttt
      721 ttaagcttcc aaaaaatttg cggaattaag ccggcattgc cgtatttcag ccacggcttt
      781 aattggttta ctttaaactg ttcaaatttt gatttattgg tcaaagtaaa aacttcaacc
      841 tgatggccta atttagacag ctctaaagcc tgatgataag caacattacc catccctccg
      901 gtgtatggcg gaaaggtgct gacaaggtga gcgattttca tattccaggg tatagattac
      961 caaaatatta cttttttaac gacctgccaa taaaggttaa aaatggggtt ggcaatgtaa
     1021 gttaaaaccg gattattaat atcctgaaat tcaactttgc cgctgaaagc tgtcaccacg
     1081 tctttatcag atagttttct tttccgctga ataattctcc ttttggctga aatatttttc
     1141 aaatttttaa aatttaacaa ataccggtaa accttcactt tctccgtcca ccagccgctg
     1201 taaatagaat aaaaaaacaa accagtttcc attaaaatca acgccgggct gattaaaaca
     1261 atggttggcc atttataatt ctgaaaaatc accataaacc ggtttctttc catataataa
     1321 tatttggcga tactccggct aaactgatat ttatgataaa caactgattg agcggccaaa
     1381 actacctggt aacccagcaa acgggctttc caggacaaat caagatcttc gtgatacata
     1441 aataaatcgt cgtcaaacat cccaattgcc tcaataaccg gccgcttaat cagcatcgcc
     1501 gcgccgctgg cgtaattgat ctgatgaacc tgccggggat aatcaaaact tgattctggt
     1561 tcctgatggc ccctggtata tccaaagcca agataattaa tcacgttacc aatggaatta
     1621 attttatctg tctgcggcca gagcattaat ctggactgga caaggccaat ttgatcatcc
     1681 gcctcagccg cctccaataa attcttcagc cagtcaggtt caacaatcgt atcctggtta
     1741 agcagaacaa aataatcaca accttccgcc atagctttcc ggatagcgta attattcccg
     1801 ccggcaaagc ccagattttc ttttaactca aatagattaa attcattaaa ctcggtttta
     1861 atataatcaa cggaatgatc gcctgaatta ttgtcaacaa taaaaacttc aaactgatta
     1921 tgatcaaaag tttgtttttt gaggctggtc agacaatcgg gcagataggc caaaccgtta
     1981 taattaacaa taatgacggc aaccataaag acaatttaag gcggaccagg ccgccttttt
     2041 atgtttaata tattttacgg attttttcaa tctgactgat cgcttttttt caatttatca
     2101 tcaatggctg ttttcctggt cagcgccgta atttcctgat caattttatc aattcttaaa
     2161 aatattttaa aaaccatata aaataaaatg gcgattgaaa tataaattat caaatcaact
     2221 ccccggccaa tgccgaccag tcgggccaga taggtagtca gttccggaaa cagaactatg
     2281 acgccgacga tcagccagac cagaacccaa aaaataaatt cccggcggtt gatggtttta
     2341 tccttatacc taacccaaat tttataaaaa acataaagga caaaaacaac tgtcaaaacc
     2401 tgaaaaatca tataataacc aatacgcgat gccaataatt gataatgata attttaaaaa
     2461 ttaagaaaat agttttttaa aaaaaatatc tttgagaata attaaggcct tgaccggatc
     2521 tgacttgtga taatctttat taagtgaatc tttggtgtat ttaacggtta ccggaaattc
     2581 agcgtagttt aatttatttc tggccagttc gccgatgatt tccgaggcgt gggaatcgcc
     2641 gtcaaaagtc agctgaattt tttgggcggc ctgacgggtc attgcccgaa aaccgttatg
     2701 ggcgtcagat aattttaagc cggaatgaaa ccattctacc aaaatagcca gcttcagacc
     2761 ggtctttcta atcagcggta tattttgggg ttttttatca agaaaccggg agccaatcac
     2821 aatatcaaag ttattattca aaagatgact gaccattggc cggatttccg ccggctcctg
     2881 ctggccgtca gcgtcaaaat gaacgatcac ttccgctccc tgattaaggg cgtaatcaat
     2941 tcctgtcttc agagcggctc cctggcc
//
LOCUS       DASWHZ010000115         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_605123, whole genome shotgun sequence.
ACCESSION   DASWHZ010000115 DASWHZ010000000
VERSION     DASWHZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605123"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VGA49_02500"
     CDS             <1..1442
                     /locus_tag="VGA49_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664669.1"
                     /translation="FLPATITGPDQPSSPPDLANLLENASRLAEESALFIPDYYPLPP
                     NSLTTVARSLKNLYPSRRALTEEFFELTEGRPFTEQLLSKLNDDLTTSSILGNIDRLV
                     VLISEEGFASFGFNKVAAGFALSPKVIIIKENQSYHTVAHEIAHTLPYLWSSSQMASQ
                     CDINYHNLEDRYAFGHQIVEAGEQNRRRQAQKISIMGSEVSASAYWMDQCTYNNLLNQ
                     LQKKVDPEIILVRGTLDRSGGTNKGQLHSLYQLDSVADLDTTSQGQWSIRLYDNQGNR
                     LARFQFDPIWKLIVHPEEIERNIISFAYRVPALPNVSQIDLVDPAGIVLDIKKISPNA
                     PLVTILRPDDESPAKVKDGKIKVRWVGEDADIGEKLLYTVFYSPDGGQTWLNQVLETT
                     ATDIEIEVDPQGLAHLVKIIVTDGFRSNEDIVSFELSASKPLVTWYVIITLIAVILIV
                     ALGYYIYRSKKEKMAETTLPATESPLPKK"
BASE COUNT          434 a          366 c          331 g          408 t
ORIGIN      
        1 acttcctgcc agcgaccatt accggacctg atcagccgtc atccccgccg gacctggcta
       61 atctgttgga aaatgcctcc cgcctggcgg aggaatcagc cttatttatt ccggattatt
      121 atcctctgcc gccaaacagt ttaacgacgg ttgcccgttc tttaaaaaat ctttatcctt
      181 cccggcgggc cttaacggaa gaattctttg aattaactga aggccggccg ttcaccgagc
      241 aattgttgag caagttaaac gacgatctga cgacttcgtc catcctgggt aacattgacc
      301 ggctggttgt gctgattagt gaagaaggat ttgccagttt tggtttcaat aaagtggcgg
      361 cggggtttgc cttgtcgccc aaagtcatta tcatcaaaga aaatcaaagt tatcacacgg
      421 tcgctcatga aatagcccat actttacctt atctatggag cagttcgcaa atggccagtc
      481 agtgcgatat taactaccat aatcttgaag accgctacgc ctttggccat cagatcgttg
      541 aagccggcga acaaaacagg cggcgccagg cgcaaaaaat ttctatcatg gggtccgagg
      601 tgtctgcgtc agcttactgg atggaccagt gtacctataa caacctgctt aatcagctcc
      661 aaaaaaaggt tgatccggaa atcatcctgg tcagagggac tttagaccgc tccggcggaa
      721 ctaataaagg tcagctccat tcgctctatc agctggattc ggtcgctgat ttagacacaa
      781 ccagccaggg acaatggtca atcaggcttt atgataatca gggcaatcgc ctggcccggt
      841 tccaatttga tccaatttgg aaattaatcg ttcatcctga agaaattgaa agaaatatta
      901 tttcttttgc ctaccgggtg ccagctctgc caaatgtttc tcaaattgat ctggttgacc
      961 cggcgggaat tgttctcgac attaaaaaaa taagccctaa cgctccatta gtaacaatcc
     1021 tccggccgga cgatgaatct ccggccaaag ttaaagacgg gaaaattaaa gtacgctggg
     1081 ttggcgaaga cgccgacatt ggcgaaaaac ttttatacac tgttttctac tccccggacg
     1141 gcggtcagac ctggctaaat caggttctgg aaacaaccgc gactgacata gaaattgaag
     1201 ttgatcccca ggggttggct catctggtta aaataattgt cactgacggc ttcagaagca
     1261 atgaagatat tgtcagcttt gaactttctg cctctaaacc gctggtgacc tggtatgtta
     1321 taatcaccct catcgctgtc attttaatag tggctttggg atattatatt tacaggtcta
     1381 aaaaagaaaa gatggcagaa acaactttac cggcaacaga atcaccactt ccaaagaaat
     1441 aaaaaatatc aatcaaaaaa gaactctccg caatgagttc ttttttattt aaaaattaat
     1501 tatttaatcc ttttcccgat atcccgggcc atttccgcc
//
LOCUS       DASWHZ010000116         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3211333, whole genome shotgun sequence.
ACCESSION   DASWHZ010000116 DASWHZ010000000
VERSION     DASWHZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3211333"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..819)
                     /locus_tag="VGA49_02505"
     CDS             complement(139..819)
                     /locus_tag="VGA49_02505"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9664670.1"
                     /translation="MVDFTNKKISDNETLGEVLRRQREELGFQLDYVSREINVQQEHL
                     LNLEDSDYQALPAPVYTRNFVELYSRFLGLVAVKMIQKYNQEVNIFDKTAKTPQKYNQ
                     PPTRLAITPRFFKSVIVGLIAAVCLVYIGFQVDTIVQPPELVIAAPNDNLVTDQLSVV
                     IDGRTEKEVKVTINGQEVLPRADGSFQENLSLKPGINLIEISAAKKYSKIKTVYRQVL
                     VEPKSAGQ"
     gene            complement(1010..3247)
                     /locus_tag="VGA49_02510"
     CDS             complement(1010..3247)
                     /locus_tag="VGA49_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9664671.1"
                     /translation="MRKKRRRYKRSSARRRQYWSEKFSVDFDLDPETKKAILAIFLFA
                     VSAVSFLSFLDLAGDAGNFISDVLKTFFGWAYFLVPVILLILGYLIINYEKYQLTFLN
                     YLGVILFVLSLTGLFHLKFNPDQAVYIINDGQGGGYLGLLVSYPLRSLMGFWASFIIL
                     LAGLAVSILLMFNNPVAWIKARLESVVFGLSRVKNFTFRKNYSDEDFEEGADQDADEV
                     IEAEFDTKKIADDAPAGRPLKKAAESLEIIPTVRISRQVEIPLDLLNNKISKPTSGDI
                     NRNSSIIQKTLENFNIPVEMGAVSVGPTVTQFTLKPAEGIKLNKITGLNNDLALALAA
                     HPIRIEAPIPGKSLIGIEVPNKGVAVVNLRELLETKNFKKRRSNLTLTIGKDVSGQAW
                     LTPLEKMPHLLVAGATGSGKSVCLNSLIISLLYQNNPDILKFVMIDPKRVELTVYNDI
                     PYLLTPVVTDVKKTVNALKWLIGEMDRRYDKLSKLGKRNIAVYNQTVAEKMPYIVVVI
                     DELADLMVSAGNEVESSIVRLAQMARAVGIHLVLATQRPSVDIITGLIKANITSRIAF
                     SVPSLMDSRTILDASGAEKLVGRGDLLFTSADLSKPVRLQGAYVSDDETRAIVDHIKN
                     NFDQPDFIEEVTDRQTSGTSFDLGGEDDDELLPEARETVVRAGKASASYLQRRLKIGY
                     ARAARILDLLEEQGVIGPADGAKPREILVTEIYEEDYHGDDQVMAEPEYEDSEEEFQK
                     DETGQ"
BASE COUNT          957 a          781 c          622 g          922 t
ORIGIN      
        1 gacaatggct gaatcttgcg tcttagccat aagattatcc ctctttaatg attaaaatcg
       61 ggatggaaat cagtgtaaag attttatcac ttcagtaaat aaaaagcaag cctgaaaaat
      121 caagatttaa ataaaatttt attggccagc gcttttgggt tcaactaaaa cctggcggta
      181 aactgtctta attttgctgt attttttggc ggctgaaatt tcaattaaat ttatcccggg
      241 tttaaggctc aaattttcct gaaaactgcc gtctgcccgc ggcaagactt cctgcccgtt
      301 aatagtcacc ttaacctcct tttccgtccg gccgtcaata acgactgaca actggtcagt
      361 caccagatta tcgttcgggg cggcaatcac cagttcgggc ggctgaacaa tcgtatccac
      421 ctgaaaacca atatagacca gacaaacagc ggcgattaaa ccaacaatga cgcttttaaa
      481 aaatcgggga gtaatcgcca gtctggtcgg cggctgatta tatttttgag gagttttggc
      541 ggttttatca aaaatattaa cttcctgatt atatttttga atcattttaa cggcgaccag
      601 ccctaaaaac cggctgtaaa gttcaacaaa atttctggtg tagaccgggg cgggcaaagc
      661 ctgataatcg ctgtcttcta aattcagcaa atgttcttgc tggacattaa tttctctgga
      721 aacataatca agctgaaacc caagctcttc tctttgtcgc ctgagaactt cgcccagagt
      781 ttcattatca gaaatttttt tgttggtaaa atcaaccata ttctttaaat tacaaatttc
      841 aaaatcccaa tgtcaaatca aatccaaaat ctcccgatgg cgaagccatc gaggatcctc
      901 gacaaagtcg ggacaaaatc caaaaatttc attatttgtc atttgagctt tggttttcaa
      961 ttgatattta ggctttgata tttagattta atagttggat ttagtgcttt tattgcccag
     1021 tctcatcttt ctgaaactct tcctctgagt cctcatattc tggttcagcc ataacttgat
     1081 catccccatg ataatcttcc tcgtaaattt ccgtgaccaa tatttctctc ggcttggcgc
     1141 cgtcagccgg gccaatcacc ccctgctctt ccagtaaatc aagaatccgg gccgcccggg
     1201 cataaccgat tttaagcctt ctctgcaaat aggaagcgga ggctttgccg gcccggacaa
     1261 ctgtttcacg ggcttccggc aaaagctcgt cgtcatcttc gccgcccaaa tcaaaacttg
     1321 ttccgctagt ctgtctatca gtcacttctt caataaaatc aggctggtca aaattatttt
     1381 taatatggtc aacaatcgcc ctggtctcgt cgtctgaaac ataagccccc tgaagccgga
     1441 caggtttgga caggtcagcc gaagtaaaca gcaaatcccc ccggccaacc agtttttcgg
     1501 cgccggaggc atccagaatt gtccgggaat ccattaaaga gggaacacta aaagcgatcc
     1561 gggaggtaat attggctttg atcaaaccgg taataatgtc aacggagggc cgctgggtgg
     1621 ctaaaaccag atgaatgccg accgcccggg ccatctgggc caggcgaaca attgaacttt
     1681 ccacttcatt gccggcagac accatcaggt cagccagctc gtcaatcacc accacgatat
     1741 acggcatttt ttcagcgaca gtctgattgt aaacagcaat attcctttta cccaattttg
     1801 acaatttatc gtagcggcga tccatctcgc caatcagcca tttcagagcg ttaaccgttt
     1861 ttttgacatc ggtaacgacc ggtgttaaca gataaggaat gtcattataa acggtcagtt
     1921 caacccgctt gggatcaatc atcacaaatt ttaaaatgtc gggattattc tgatacagca
     1981 aactgataat aagcgaattc agacaaacac ttttacctga accggtcgcc ccggccacca
     2041 acaaatgggg cattttttcc aagggtgtca gccaggcctg cccgctgacg tctttgccaa
     2101 tcgttaaagt taaatttgaa cgacgttttt taaaattttt ggtttccagt agttccctta
     2161 ggttaacgac cgccaccccc ttattgggaa cttcaatgcc aatcaatgat ttgccgggaa
     2221 tcggcgcctc aattctgatt ggatgagccg ccaaagccag cgccagatca ttattcaatc
     2281 cggtaatttt atttaatttg attccttcgg ccggctttaa agtaaattga gtgactgtcg
     2341 gcccgacgct gaccgcgccc atttcaaccg gaatgttaaa attttccaaa gttttctgaa
     2401 taatcgaaga atttctgtta atatcgcctg aagtcggttt gctaattttg ttattaagca
     2461 gatcaagcgg aatttcaacc tgacgactga tcctgaccgt tggaatgatt tccagcgatt
     2521 cagccgcttt tttcaaaggc ctgccggctg gcgcgtcatc agctattttt ttggtgtcaa
     2581 attccgcttc aataacttca tcggcgtctt gatccgcccc ttcttcaaaa tcttcgtcgc
     2641 tgtaattttt tctaaaagta aaatttttaa ccctgctcag tccaaaaacg actgactcca
     2701 gtctagcttt gatccaggcg accggattat taaacatcag caggattgaa acagccaaac
     2761 cagccagtaa aattataaac gacgcccaga atcccattaa actccgcagc gggtaactga
     2821 ccagcaaacc aagatagccg ccgccctgac cgtcattaat aatatatacc gcctgatcag
     2881 ggttaaattt cagatgaaac aggccggtta ggctcaaaac aaacaaaatc acccctaaat
     2941 aattcagaaa agtcagctga tatttttcat aattgatgat caggtaacca agaattaata
     3001 aaataaccgg gactaaaaaa taagcccagc caaaaaaggt tttcaaaaca tcgctgataa
     3061 aattaccagc gtcacccgcc aaatccaaaa aactcaaaaa agaaacagct gaaacagcaa
     3121 acagaaaaat ggccagaatg gcttttttag tttccggatc aagatcaaaa tccacgctga
     3181 atttttctga ccaatattgg cggcgcctgg ctgatgatct tttatatctt cttctttttt
     3241 ttctcataat ttatcttatt ataacaaaaa aaagaccctt ca
//
LOCUS       DASWHZ010000117         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_233636, whole genome shotgun sequence.
ACCESSION   DASWHZ010000117 DASWHZ010000000
VERSION     DASWHZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233636"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGA49_02515"
     CDS             <1..195
                     /locus_tag="VGA49_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664672.1"
                     /translation="QIKEDYEVVQKAISKGGLEALSSKMGKFIQPRTKGQGGASKKTR
                     AFYARTNFIEVIFGMREYIE"
     gene            390..764
                     /locus_tag="VGA49_02520"
     CDS             390..764
                     /locus_tag="VGA49_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664673.1"
                     /translation="MKKEQKMTPELAVKTINHIQKVFYVVGALNVFFATLILVTSDFN
                     VYAGSLIISILIGLAIVFMGWALNKPYRYKIPKAAFILSALLLVVNIINLSIVGLIFP
                     VALWIIGKQSQRAITVLENLKK"
     gene            813..2072
                     /gene="ahcY"
                     /locus_tag="VGA49_02525"
     CDS             813..2072
                     /gene="ahcY"
                     /locus_tag="VGA49_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9664674.1"
                     /translation="MKHHIKSIKLAQEGKKRIDWAEQQMPVLGFIKKRFAKEKPLKNL
                     KIAACLHITAETANLARTLKAGGAAVVLCASNPLSTQDDVTASLVKDYQISVFAINGE
                     DKKTYYSHLEAALKTKPRITIDDGADLISLLHSKFKKQTDQIWASLEETTTGVIRLRA
                     LEKSGQLKLPVIAVNDAKTKYLFDNRYGTGQSTIDGIIRATDILIAGKNVVIAGYGWC
                     GRGLAMRARGLGAKVIITEVDPIKAIEAALEGFEVMTMDQAAPLGDLFCTLTGNIDVI
                     NKKHFSRMKDGAIVCNSGHFDVELNLKDLKKLARQIKKNVRQFVDQYTLKNNKNIYLL
                     AEGRLINLVAAEGHPASVMDMSFATQVLAAEYVAKQKDKLQSKIYNLPQDLEYKIAKL
                     KLQSMGLTIDQLTSAQKKYLSSWQIGT"
BASE COUNT          770 a          420 c          518 g          680 t
ORIGIN      
        1 cagatcaaag aagattacga ggtagtacaa aaggctatta gcaaaggagg gctggaagcg
       61 ttatccagca aaatgggcaa gtttattcag ccacgaacga aaggacaggg tggagccagt
      121 aaaaaaaccc gcgcgtttta cgcacgtaca aattttattg aagtgatctt cggaatgagg
      181 gaatacattg aataaaaata cataggttat tgaaaatttc gtttgaaaat attatatcgc
      241 ctctctcact tcgcaacttc agatattccg gaacgttagg ctccataata aggagtcgat
      301 tggagttcga aataatgatt ttaataattt attgttgagg gcgaggataa ctatgacata
      361 atcaaaataa taattaattt acataaatta tgaaaaaaga gcaaaaaatg acacctgaat
      421 tagcggtaaa aacaataaat catattcaaa aagtctttta tgttgttggt gctttgaacg
      481 tgttttttgc cactctcata cttgttacaa gcgactttaa tgtttacgcc gggtcattaa
      541 ttataagtat tcttattgga ttggcgattg tattcatggg atgggcatta aataagccat
      601 atagatataa aattccaaag gctgccttca ttttaagtgc tttgctgtta gttgtaaata
      661 taataaatct ttctattgtc ggtttaattt tcccagtagc attatggatc attggtaaac
      721 agtcacaacg tgcaattaca gtgcttgaaa atttaaaaaa ataaattaca atcattaaca
      781 aataggattt ttatcagtca tttgacttta gtatgaaaca tcacatcaaa tcaatcaaat
      841 tagcccaaga gggcaagaag cgcattgact gggcagaaca gcagatgccg gttttaggtt
      901 tcataaagaa aagatttgcc aaagaaaaac ctttgaagaa tcttaaaatt gccgcctgcc
      961 tgcatattac agctgaaacc gccaatctgg cccggacttt gaaagctggc ggggcggcag
     1021 tggttttatg cgcttccaac ccattatcaa cccaggatga tgttaccgct tcattagtca
     1081 aagattatca aatatcagtg tttgccatta acggcgaaga caaaaaaact tattattctc
     1141 atttagaagc ggctttgaaa actaaaccgc gaattacgat tgatgacggg gctgatttga
     1201 tttctttgct tcattcaaaa tttaaaaaac aaactgatca aatttgggcc agtctggaag
     1261 aaaccactac cggggtgatc cggttaagag ctttggaaaa aagcggtcag ttgaaattgc
     1321 cggtgatcgc ggtcaatgac gctaaaacca aatatttgtt tgacaaccgt tatggcaccg
     1381 gtcagtcaac tattgacggc attatccggg cgactgatat tttgatcgcc gggaaaaatg
     1441 tcgtgattgc cggctatggc tggtgcggcc ggggtttggc catgcgcgcc cggggcttag
     1501 gggccaaagt gattattact gaagttgacc cgatcaaagc gattgaagcg gctttggaag
     1561 gctttgaagt gatgacgatg gatcaggccg ctccgctggg tgatttattc tgcaccttga
     1621 ccggcaatat tgatgtcatt aataaaaaac attttagcag aatgaaagac ggggcgattg
     1681 tctgcaattc aggccatttt gatgttgagc ttaatttgaa agatttgaaa aaactggcca
     1741 ggcagattaa aaaaaatgtc cggcagtttg ttgaccagta tactttgaaa aacaacaaaa
     1801 atatttattt gctggccgag ggccggctga tcaatttagt ggctgctgaa ggccatccgg
     1861 cttcagtaat ggacatgagt tttgccaccc aggttctggc ggctgaatat gtcgcgaaac
     1921 aaaaagataa acttcagtcc aaaatttata atttacccca ggatcttgaa tacaaaatag
     1981 ctaaattaaa acttcagtca atgggtttaa caattgatca gttaacttcc gcccaaaaaa
     2041 aatatttatc ttcctggcag ataggaacct gatttggtat ctggtcgtta gtaattagta
     2101 gttggtatta atcaataact aaaaaccaat taccaactac caagcaccaa gaaccattct
     2161 agttgacttt taaaaaaaat tgtgattaaa tgataagtta gttttcaacc aaccgttctt
     2221 tgtggggagg gaaaaatgaa gtgcgccaat atccggtgcc ataaccaact gccggcattt
     2281 agcctgaccg gtccggttaa aaagggtgat gagctggttt tttttgttga cgtgcaggtt
     2341 atacaggcca aaggttttag ccgtcagccg gttgagtgca tgagagag
//
LOCUS       DASWHZ010000118         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2832474, whole genome shotgun sequence.
ACCESSION   DASWHZ010000118 DASWHZ010000000
VERSION     DASWHZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832474"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VGA49_02530"
     CDS             <1..876
                     /locus_tag="VGA49_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664675.1"
                     /translation="AKNDPDVKIVESLITKILEQHGGIMEENHLVENVLVYPGENELN
                     QASLLFLVSKILNDKFSKVSKRQEFLPAWKLKLADDSGHLNLINRIKEVINNNQEPLE
                     EKLIIEKAKAGLADGLADDNLIIGSLKLAKDLKQDIFGCWGLVSWSSVTPKRMNDKIY
                     IVLKQHAKPLHFTEIATMINDMKFDKKTAYPATVHNELILDNKYVLVGRGIYALSDWG
                     YKPGVVVDVIADILKKENQPVSRDEIVKKVLEQRIVGKSTIYLALMNKDKFQKIDSGQ
                     YQLTDWSADSEITTV"
     gene            912..2363
                     /locus_tag="VGA49_02535"
     CDS             912..2363
                     /locus_tag="VGA49_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664676.1"
                     /translation="MTIFDPEFWLATGAKPLPVMIWEIFKNGGWIIFDLAFLFMLKGL
                     WKKYVQGIYGAKRKFIVLAIDVPKDNEKTPKTAENIFSQLAGAHGSTTKWEEWWDGEV
                     QDTFGLEIVSLDGYVQFLIRTRDKFKDLVEASIYAQYPEAEITEVADYAEAVPHKYPN
                     DTHDFFGLEYTLHKPECYPLKTHIDFIDKATGDFIDPMAALLEIMSKIGQGEQIWIQF
                     VITPIDDKWKTGGEKVVQKLVGAKVEAKKNLLDHLIDVPLNILSGIGDILTPGEAVPS
                     RPQRDEPSKILYLTPGESSAVEAIERKISKLGFKTKLRIMYIARKEVFDKRRGVNPVV
                     GAFKQFNTIDLNRFAVVKKVWTKADYAFAKTRKRAKQNKLMQAYVNRSNWRGWGTGVV
                     YNIEELASMYHFPVIAVRAPLVKKIGAKKGEPPVGLPIEDVELPREPRVTKYSKKYFE
                     EKEMAGSGQSAKEAGPGHTPGAEIEPPVNLPIE"
     gene            2383..>2970
                     /locus_tag="VGA49_02540"
     CDS             2383..>2970
                     /locus_tag="VGA49_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9664677.1"
                     /translation="MADSNKEKITFLAQTNFRNVRRKFGIKPDDRRRHLYLIGKTGMG
                     KSTTLENMIIQDIQAGHGLAVVDPHGDLAEKIVDFIPSDRINDTIYFNPADMDYPIAF
                     NVLEKVDSHYRHLVASGLIGVFKKIWADSWGPRLEYVLRNAILALLDYPDSTLLGVTR
                     LLVDKEYRKKVISKISDPVVRSFWVDEYSKYPDKFA"
BASE COUNT          907 a          583 c          696 g          784 t
ORIGIN      
        1 gctaaaaacg acccggacgt taagatagtt gaaagcctga ttaccaaaat tctggaacag
       61 cacggcggga ttatggaaga aaatcatctg gtggaaaacg ttctggttta ccccggtgaa
      121 aacgaactga accaggcttc attgttgttt cttgtttcca aaattttaaa cgacaaattt
      181 tcaaaagtca gcaagcggca ggaattttta ccagcctgga aattgaaatt agcggacgac
      241 agcggtcatc ttaatttaat taacaggatt aaagaggtca tcaacaacaa ccaggagccg
      301 ctggaggaaa aattaattat tgaaaaagcg aaagccggtc tggccgacgg cctggcggat
      361 gataatttga ttattggcag tttaaaatta gccaaagatc taaagcagga catttttggc
      421 tgctggggcc tggtcagctg gagttccgtt actcccaaaa gaatgaacga taaaatttat
      481 attgttttaa aacagcacgc caaaccgctt catttcacgg aaatcgccac aatgattaat
      541 gacatgaaat ttgacaagaa aaccgcttat ccggcgactg ttcataacga actgattctg
      601 gacaataaat atgtcctggt cggccggggg atttacgctt tgtctgactg gggctacaaa
      661 ccgggcgtgg tagttgatgt tatcgcggat attttgaaaa aagaaaatca gcctgtatcc
      721 cgggacgaaa tcgttaaaaa agttttggag cagagaattg tcggcaaaag cactatttat
      781 ttggctttaa tgaataagga caaatttcaa aaaattgatt caggtcagta tcagctgaca
      841 gactggtcag ctgattccga aatcaccact gtctaaaatt ttgacagtta ttaaaaatac
      901 aaacacaaat tatgacaatt tttgatcctg aattttggtt ggcgactggc gccaagccgt
      961 tgccggtgat gatctgggaa attttcaaaa acggaggttg gattatattt gacctcgctt
     1021 ttttatttat gctgaaagga ctctggaaaa aatatgttca gggaatttac ggcgctaaac
     1081 gcaaattcat tgttctggcg attgacgtcc caaaagacaa cgaaaaaaca cccaaaaccg
     1141 ccgaaaacat tttcagccag ctggccggcg cccatggcag taccactaaa tgggaggagt
     1201 ggtgggatgg tgaagttcag gatactttcg gtctggaaat cgtcagtctt gacggttatg
     1261 tccagttttt aatcagaacc agggataagt tcaaagattt ggtggaagcc tcaatttacg
     1321 ctcaatatcc ggaggcggaa attactgaag tagccgatta cgctgaagcc gtccctcaca
     1381 aatatcccaa cgacacccat gattttttcg gtctggaata tactttgcac aagccggaat
     1441 gttatccttt aaaaacccat attgatttta ttgataaagc taccggagat tttattgacc
     1501 caatggccgc gttactggaa attatgagca aaatcggcca gggggagcag atttggattc
     1561 agtttgtaat cacgccgatt gatgataaat ggaaaaccgg cggtgaaaaa gtcgttcaaa
     1621 aattggttgg cgccaaagtt gaagccaaaa aaaacttact ggatcatttg attgatgtcc
     1681 cgctgaatat tttatcgggc attggcgata tcctgacccc gggcgaggcc gttccttcca
     1741 gaccgcaaag agacgaaccg tcaaaaattt tgtatttgac accgggggaa tcgtctgccg
     1801 ttgaagcgat tgaaagaaaa atttccaagc tcgggttcaa aactaagttg agaataatgt
     1861 atattgcccg caaagaagtt tttgataaac ggcggggagt caacccggtg gttggggctt
     1921 tcaagcagtt caatacaatt gatttgaacc ggtttgccgt cgttaaaaaa gtctggacaa
     1981 aggctgatta cgcttttgcc aaaacaagaa agagagctaa acaaaataaa ttgatgcagg
     2041 cttatgtcaa ccggagtaac tggcggggct gggggacggg cgttgtttat aatattgaag
     2101 agctggccag catgtatcac tttccggtta ttgccgtcag agccccgctg gttaaaaaga
     2161 ttggcgctaa aaaaggcgaa ccgccggttg gtctgccaat tgaagatgtt gaactgcccc
     2221 gggagccgag ggttacaaaa tattcaaaaa aatattttga agaaaaggaa atggctggat
     2281 cgggacagtc cgccaaagaa gccggtcccg gacacactcc tggcgctgaa attgaaccac
     2341 cggttaattt accgattgaa taatttagta taaacttcat ctatggccga cagcaacaaa
     2401 gaaaaaatta cttttcttgc tcaaacaaac ttcagaaatg tccggcgaaa gtttgggatt
     2461 aaacctgatg atcgccgccg gcatctgtat ttgattggta aaaccggcat gggaaaatca
     2521 actaccctgg aaaatatgat cattcaggac attcaggccg gacatggttt ggcggtagtt
     2581 gatccccatg gtgatttggc tgaaaaaatt gttgacttta ttcccagcga ccggattaat
     2641 gatactattt acttcaatcc agccgacatg gattatccaa ttgcttttaa tgtcctggaa
     2701 aaagttgatt ctcactaccg gcatttggtg gcctccggct taatcggtgt ttttaaaaaa
     2761 atctgggctg actcctgggg accgcgcctg gaatatgttt taagaaacgc cattttggct
     2821 ctgctggatt atcctgacag cacgcttttg ggagtcaccc ggctgctggt tgataaagaa
     2881 taccgcaaaa aagtgatatc taaaatttct gatccggtgg tcaggtcttt ctgggttgat
     2941 gaatactcca aatatcctga taaattcgcc
//
LOCUS       DASWHZ010000119         2877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2836789, whole genome shotgun sequence.
ACCESSION   DASWHZ010000119 DASWHZ010000000
VERSION     DASWHZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2836789"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="VGA49_02545"
     CDS             <1..1134
                     /locus_tag="VGA49_02545"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HEX9664678.1"
                     /translation="LAEVMMAGDAKGRVFTFPIPTYNITKNIDWDSPVLDKVFEMTAK
                     YGVPYFSNFINSDMDPDDARSMCCRLRLDNRELRKRGGGLFGANPLTGSIGVVTLNLP
                     RIGYLAKNKEEFYQRLEKMMDLAKESLEIKRKFVESFTDQGLFPYCKFYLSSIKEGFD
                     SYWKNHFNTIGLIGMNEACLNLIGQDITTPEGGSFAAGGLDFMRNRLMDYQEETGSLY
                     NLEATPGEGTAYRLAKIDKEKYPTIIVANQKACQESGKAPYYTNSSQLPVGYTDDMFS
                     ALDIQDGLQTRYTGGTVFHGFIGESLPSVASTKSLVRKIAENYSLPYYTITPTFSICP
                     NHGYLSGEHFFCPKCDEAVGYKSEQPAGELANLEVKQTESVKQ"
     gene            1264..1401
                     /gene="nrdD"
                     /locus_tag="VGA49_02550"
     CDS             1264..1401
                     /gene="nrdD"
                     /locus_tag="VGA49_02550"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEX9664679.1"
                     /translation="MREQKSKRQPCEVYSRVVGYLRPVQQWNDGKQAESDDRVEYTVK
                     K"
     gene            1404..2102
                     /locus_tag="VGA49_02555"
     CDS             1404..2102
                     /locus_tag="VGA49_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEX9664680.1"
                     /translation="MRIGGLQKMSLIDYPGKISAIIFTQGCVWRCGYCHNPELVDPGQ
                     FQPALSQSELLDFLADRKKLLDGVVITGGEPTLHPDLIGLIREIKELGYAVKLDTNGI
                     FPVRLKEIAEAGLVDYIAMDIKAPPGSYQAVVNAPRFDFKNVLESIEIIMGSGIDYEF
                     RSTMIDGIHKKKDVISMVNLIKGAKRYYLQKFRNQGKMLDQTFKKFRAFTDREMAEMV
                     RLCRPYVENCEARG"
     gene            complement(2129..>2877)
                     /locus_tag="VGA49_02560"
     CDS             complement(2129..>2877)
                     /locus_tag="VGA49_02560"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEX9664681.1"
                     /translation="QTDIRDSSQVQKALDNLKPDVVLNAAGITGRPNVDWCEDPKNKF
                     ETFEVNVLGAVNIAKACDQFGIYLAHIGSGCIYQGNNDGQGFTEDDPQNFFGSFYSRT
                     KGLSEDLLKPFNPLQLRVRIPIESAPSPKNVIDKLLKYEKIISVENSFTVVPDFLKAS
                     YALIDRKEKGIFNMTNAGSTDHQYIMDKYKEIVDPDYNYALMSLDELKQSTVAERSNC
                     VLNTDKRENLGIKMPPIKERIIEVLREYKK"
BASE COUNT          809 a          593 c          677 g          798 t
ORIGIN      
        1 ctggcggaag tgatgatggc tggcgacgcc aaaggccgtg tttttacttt cccgattcca
       61 acttacaata tcactaaaaa tattgactgg gacagcccgg tgctggataa ggtttttgaa
      121 atgaccgcca aatacggcgt gccttatttt tccaacttta tcaattctga catggatcct
      181 gacgacgccc ggtcaatgtg ctgccgtttg cgtctggata accgggaatt aagaaaacga
      241 ggcggcggtt tgtttggcgc caatcctctg accggctcaa ttggcgtggt tactctgaat
      301 ctgccgcgga tcggctattt agctaaaaat aaagaagaat tttaccagcg gctggaaaaa
      361 atgatggacc tggctaaaga aagtctggaa attaaaagaa aattcgtgga aagttttact
      421 gaccaggggc tgtttccata ttgtaaattt tatctgtcaa gcattaagga aggttttgat
      481 tcgtactgga aaaatcattt taatactatt ggcctgatcg gcatgaatga agcctgcctg
      541 aatttaatcg gccaggacat taccacgccg gagggcggta gctttgccgc cggagggctg
      601 gatttcatga gaaaccggct gatggattat caggaagaaa ccggatcttt gtataatctg
      661 gaagccacgc ccggcgaggg gactgcttac cgactggcta aaattgataa agaaaaatat
      721 ccgacaatta tcgtcgctaa tcaaaaagct tgccaggagt cagggaaggc tccttattac
      781 accaattcat cccagctgcc ggtcggctat actgacgata tgttttccgc tttggatatt
      841 caggatggtc tgcagacccg ttatactggc gggacggttt ttcacggctt tatcggtgaa
      901 agcctgccgt ctgtcgccag caccaaaagt ttagtccgta aaatagctga aaattacagt
      961 ttgccttact acaccataac gccgactttc agcatctgtc ccaatcacgg ctatttgtcc
     1021 ggcgagcatt tcttttgccc gaaatgcgat gaagcggttg gctataaatc agaacaacca
     1081 gccggcgagc tggctaattt agaagtcaaa caaaccgagt cagttaaaca ataaataaca
     1141 gtcaatagcc aacagccaac agggtcattt gtccttgcct aaaacataaa ccctaagacc
     1201 tgacctgttg gcggatgact gttgactaat gactaattaa aattaataag aaaggatata
     1261 aatatgcggg aacaaaaatc aaaaagacag ccctgtgaag tctattcaag agtggtcggc
     1321 tatctgcggc cggttcagca gtggaacgac ggtaaacagg ccgaatctga cgaccgggtt
     1381 gaatacactg tcaaaaaata gttatgcgaa tcggcggact gcagaaaatg tcgctgattg
     1441 attatccggg aaagatttca gccattatat ttacccaggg ttgcgtctgg cgttgcggtt
     1501 attgccataa cccggaactg gttgatcccg gccagtttca gccagctttg agccagtcag
     1561 agctgcttga ttttttggct gaccggaaaa agctgctgga cggggtggtc atcaccggcg
     1621 gcgagccgac gctccatcct gatttaatcg gtttaatccg cgaaattaaa gaacttggtt
     1681 acgctgttaa actggacacc aacgggattt tcccggtccg gttaaaagaa attgctgaag
     1741 ccgggctggt tgactatatt gccatggata ttaaagctcc gcccggcagt tatcaggctg
     1801 ttgtcaacgc cccgcgattt gattttaaaa atgttttgga atcaatagaa attattatgg
     1861 gctcgggcat tgattatgaa tttcggtcaa cgatgattga cgggattcat aagaaaaaag
     1921 atgtcatcag catggttaat ttaatcaaag gcgctaaaag atattatttg caaaaattca
     1981 gaaatcaggg aaaaatgctg gatcagactt ttaaaaaatt ccgggcgttt accgaccggg
     2041 agatggctga aatggttcgg ctttgccggc cgtatgtgga gaattgtgaa gcgcggggct
     2101 gactatggag caggcttcgc gataattttt attttttata ttcccgcaaa acttctataa
     2161 tcctttcctt aatcggcggc atcttgatgc ccagattctc gcgtttatca gtgtttaaaa
     2221 cacagtttga tctttcagcc acagtggatt gtttaagttc gtctaatgac attagagcat
     2281 aattgtaatc aggatcaaca atttctttat atttatccat tatatattgg tggtcagtcg
     2341 aaccggcatt agtcatgtta aaaattcctt tttctttcct gtcaatcaaa gcataagaag
     2401 ctttcagaaa atcaggcacg actgtgaaag aattttcaac tgaaataatt ttttcgtact
     2461 ttaaaagttt gtcaatgaca tttttgggcg agggggcgct ttcaatcgga attctgactc
     2521 ttaattgaag aggattgaat ggttttaata aatcttccga cagccctttg gtccgggaat
     2581 aaaatgagcc gaaaaagttt tggggatcat cttcagtaaa tccctggcca tcattattcc
     2641 cctggtaaat acagccgctg ccgatgtggg ccagataaat accaaattga tcgcaggctt
     2701 tggcgatatt gacggcgccc agaacattaa cttcaaaagt ttcaaattta ttttttggat
     2761 cttcgcacca gtcaacattg ggccggccgg taatgccggc ggcgtttaaa acgacatctg
     2821 gcttaaggtt atccaaagct ttttgaacct ggctggaatc gcgaatgtcg gtctgtt
//
LOCUS       DASWHZ010000120         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_239604, whole genome shotgun sequence.
ACCESSION   DASWHZ010000120 DASWHZ010000000
VERSION     DASWHZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239604"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..2428)
                     /locus_tag="VGA49_02565"
     CDS             complement(98..2428)
                     /locus_tag="VGA49_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9664682.1"
                     /translation="MTLQGWKRIIFPDDPNPLFFVISDEAYQAMVKRGMLQMSPDQTL
                     PAKLQSGGPAYIPLKKLDEGGMGIVLVVTDIFGRQVVLKVIKGAATDDKKERFIQEAK
                     LTAGLQHPNIIPVHDLGLRDGHPYFTMKRIYGQSFKQVLEEDRGDYHLSELLRSFLKI
                     CEAVAFCHSKEVIHRDLKPANVMIGQFGEVLLMDFGLAKYIGQGEPYFEAINPPEEPE
                     GFIVGSPKYMSPEQAEGRDDVGFPTDVYGLGVILFEILTGTVPFVANDAMSILTKVIR
                     DPAPDPRVLNPAVSKELALITLKAIAKNQADRYQTVEELMADIEAYQDGRDVSVMTYT
                     KTDRVKRYVKRYPGRAIAAVWVMLALTVVGVGFGVLTSWAKREMAQRLVAEKLAREQE
                     ELARQTAEEKAEAEERARQEAEKREKIETERRQELEKRTRAFAIYLEAADLHDRFQYQ
                     LALDKVNQAIEIDDSFQEAFYLRGMIYQDLNQAAEAIADFQRAFELSRAQGLVLTSAL
                     YRVGATYLYLLNDFTRAQEVFVQAEQLGMTDDPFILIMKAHIDVPTGRLAAALDKLDR
                     AAELADHLFEVHFTRGYIYLQYVHRRQLDYLEPAIDAFSQTLKLNYRHLAAYGNRAQL
                     LALANRPEEALADYRTALSMYPDNAQLRYNRGVFHKDSYQFDPAQADFEKALELDQNF
                     ALAYDGLGSVYTLRGMAADNQPEKNRFFDQADVWFEEALRRNAPTAYINRGLLRYHQG
                     RPREALDWLERSIQFNPGRRQWIEAQMEMIRNTRED"
     gene            2706..>4220
                     /locus_tag="VGA49_02570"
     CDS             2706..>4220
                     /locus_tag="VGA49_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9664683.1"
                     /translation="MQNLQKLSSFISKETSFNFIKALLKEFPTAEIFLVGGAVRDFAL
                     GNQDVNDYDFVVRNVSAKKLSSFLKKHGLVNLVGRHFGVFKFVPTPVARGRRGQKLSW
                     EAFDIALPRTEHSLGTSGGYKDFEIQSNPKLPIELDLSRRDFTINALAWDLGKNELVD
                     LFDGLKDLKKKIIRTVGQPKIRFAEDYSRMLRAVRFACQLDFEIDKPTWQFIKKNINL
                     INKTKNNDRVVPYETIAKELLKAFYFNPVKALDLFDASNIIKETMPELLKMKKCIQPK
                     NWHTEGDVWRHTRLCLSLMNSAKFKKRFGAEKPGAQLIMGVLAHDLGKPYTIQTPKKD
                     KVDRIRYYNHDTVGAELFSKICKRLKLSSPPHYGIDCDQTAWLIKNHLVDVQIKEMKN
                     TTVEKYFLNPGVPGNDLIKLLYLDAQGTIPPSGRPDMGTFNILMKRIKEIKKLPRKKT
                     QKITPPILNGHEIIKKFKLKPGPRIGELLDALREQQLTGKIKTKIQAAQFLKNYL"
BASE COUNT         1141 a         1080 c          948 g         1051 t
ORIGIN      
        1 atttcggatt tatataaatt aggatttcaa aatataaggt ttaaatattt attttcttaa
       61 ttaaaaaagc tcctccgaca agcggggagc tttgtgatta atcctccctg gtgtttctga
      121 tcatctccat ttgggcctca atccactggc gacgaccggg attgaactga atggaccgct
      181 ccagccagtc cagcgcctcc cggggccgtc cctggtgata gcggagcagt ccccggttaa
      241 tgtaagctgt cggcgcgttt cgccgaagag cctcctcaaa ccagacatcg gcctggtcaa
      301 aaaaccgatt tttctccggc tggttgtcgg ccgccatgcc tcgcagggtg taaactgacc
      361 ccaaaccgtc gtaagccaga gcgaaatttt gatcaagctc aagcgctttt tcaaaatcag
      421 cctgggccgg atcaaactga taagaatctt tgtgaaacac tccgcggtta tagcgaagct
      481 gggcgttgtc cgggtacatt gacagggccg tccggtagtc cgccaatgct tcctccggcc
      541 ggttggccaa agccagcagt tgggcccggt tgccataagc ggccagatgc cggtaattga
      601 gtttcagcgt ctggctgaaa gcgtcaatcg ccggctccag atagtccagc tgtcggcgat
      661 gaacatactg gagataaata tagccgcggg tgaaatggac ttcaaacagg tggtcagcca
      721 gttcagccgc ccggtccagt ttatccagag ctgccgccag ccggccggtg ggaacatcaa
      781 tatgggcctt cattatcaaa ataaagggat catctgtcat tcccaactgt tccgcctgga
      841 caaagacttc ctgggcccgg gtaaaatcgt ttagcaaata gagataggtg gcgccaaccc
      901 ggtacaaagc gcttgtcaac accagcccct gggctcgcga caattcaaaa gcccgctgaa
      961 aatcagcaat cgcttccgcc gcctggttaa gatcctggta gatcatgccc cggagataga
     1021 aagcttcctg aaaactgtcg tcaatctcaa tcgcctggtt gaccttgtcc aaagccagct
     1081 gatactggaa acgatcatgg agatcagccg cctccaggta gatcgcgaaa gccctggtcc
     1141 gcttttccag ctcttgacga cgttcggttt ctatcttttc ccgcttttca gcttcctgtc
     1201 gggcgcgctc ttcagcctcg gctttttctt cggctgtttg ccgggccagt tcctcctgtt
     1261 ccctggccag tttttcagcc accagccgct gggccatttc tcgtttagcc caggaagtca
     1321 gaacgccaaa gcccacgccg acaacagtca gagccagcat cacccaaacc gcggcaatcg
     1381 cccggccggg atatctttta acataccgct taacgcggtc ggtcttcgtg taggtcatca
     1441 ccgacacatc ccgcccatcc tgataagcct caatgtcagc catcagctct tccacggttt
     1501 gataccgatc cgcctgattc ttggcaatgg ctttcagggt aatcagggcc agttctttgg
     1561 aaacagccgg gttaagtact ctggggtctg gcgccgggtc ccgaatcact ttggtcagaa
     1621 tactcatggc gtcattggcc acgaacggaa ccgtgccggt cagaatttca aacagaatca
     1681 cgcccaagcc ataaacgtca gttggaaaac caacatcatc ccggccttcg gcctgttccg
     1741 gcgacatata tttaggtgat ccaacaatga atccctccgg ttcttccggc ggattgatcg
     1801 cctcaaagta tggctcgccc tgtccaatgt atttggccag gccaaaatcc atcagcagga
     1861 cctctccaaa ctggccgatc atcacgttgg ccggcttgag gtcgcggtgg atgacttctt
     1921 tggagtggca aaaagccacc gcctcgcaga tcttcaggaa actccgcaac aactcggaca
     1981 gatggtaatc accacgatct tcttccaaaa cctgcttaaa ggattggccg tagatgcgtt
     2041 tcatagtgaa gtaggggtga ccgtcccgca atcccagatc gtggaccgga ataatgttgg
     2101 gatgctggag cccggctgtc agcttggctt cctgaataaa ccgttctttt ttatcgtcag
     2161 tggccgcgcc tttgataacc ttcagcacga cctgacggcc aaagatgtca gtcaccacca
     2221 gcacaatgcc catgccgcct tcatccagct ttttcaaagg gatgtaggct ggcccgccgg
     2281 actgaagctt ggccggcaaa gtctgatccg gagacatctg gagcattcct cgtttaacca
     2341 tcgcctgata ggcttcatca gaaatgacga agaacagggg gttgggatca tcaggaaaga
     2401 tgatccgttt ccaaccttga agcgtcatct ttcctccttg ggttgatccc gccaaaggcg
     2461 gaatgttttt caacgaacac cactattgta acaaaaaagt atagccaaag ttatctaaaa
     2521 agtcaatctt tggcttgtta ttagtctaaa attgagatat aattaggtaa gaagttaaaa
     2581 gtaaaaagta aaaaatttgg tatcgctgcg ccaggccatt gcctggcttc gcgataattt
     2641 taaataactt ttgatttttg acttgtactt tttacttata acttattact ttttactttc
     2701 gcattatgca aaacctccaa aaactttctt cttttatcag caaagagaca tccttcaact
     2761 tcatcaaagc cctgctgaaa gaatttccaa cagcggagat ttttttagtg ggcggggcgg
     2821 tcagggattt tgctttgggc aatcaagatg tcaatgacta tgatttcgtc gtcagaaatg
     2881 tctccgccaa aaaattaagc tcctttttaa aaaaacacgg cctggtcaat ctggtcggcc
     2941 ggcattttgg ggtatttaaa tttgttccta cccctgtcgc ccgcgggcga cggggccaaa
     3001 aactgtcctg ggaagcgttt gacattgccc tgccccggac tgaacattct ttggggacaa
     3061 gcggcggcta taaagatttc gaaatccaat caaatcccaa actgccgatt gagctggatt
     3121 tgtccaggcg ggatttcacg attaacgccc tggcctggga cttaggcaaa aatgaactgg
     3181 ttgacctttt tgacggcctg aaagatttaa agaaaaaaat tatccggaca gtcggccagc
     3241 ctaaaatcag attcgctgaa gattattccc gaatgctccg ggcggtccgc tttgcctgcc
     3301 agcttgattt tgaaattgac aaaccaacct ggcagttcat caaaaaaaat atcaatctta
     3361 ttaataaaac aaaaaataat gaccgggttg tcccttatga aacaatcgcc aaagaactgc
     3421 tcaaagcttt ttattttaac ccggtcaaag cactcgattt atttgacgcc agtaatatca
     3481 tcaaagaaac catgcccgaa cttttaaaaa tgaagaaatg tatccagccc aaaaactggc
     3541 acactgaagg cgatgtctgg cggcacaccc ggctatgttt atcgttaatg aattcggcta
     3601 aatttaaaaa aagatttggg gctgaaaaac ccggcgctca attaattatg ggcgtcctgg
     3661 ctcacgacct gggcaagccc tacaccattc aaactcccaa aaaagataaa gttgaccgaa
     3721 tccgttacta taatcacgac actgttggcg ctgaactatt ttcaaaaatt tgtaaaagat
     3781 taaaactatc cagcccgcct cattacggca ttgactgcga tcagactgcc tggctgatta
     3841 aaaatcatct ggttgacgtt cagattaaag aaatgaaaaa cacgacggtt gaaaaatatt
     3901 ttttaaaccc cggagtcccg ggaaatgatc taatcaaact gctatatctc gacgcccagg
     3961 gaacgattcc cccgtcaggc cggccggaca tgggaacttt taatatttta atgaaacgaa
     4021 tcaaagaaat taaaaaactg ccccgaaaaa aaactcaaaa aattacaccg ccgattttaa
     4081 acggccatga aattattaaa aagttcaaat taaagcccgg cccgcgaatt ggcgaattac
     4141 ttgatgcttt gcgggaacag caattaaccg gcaaaattaa aaccaaaatt caggcggctc
     4201 aatttctaaa aaactatctg
//
LOCUS       DASWHZ010000121         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3553395, whole genome shotgun sequence.
ACCESSION   DASWHZ010000121 DASWHZ010000000
VERSION     DASWHZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3553395"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3413)
                     /locus_tag="VGA49_02575"
     CDS             complement(<1..3413)
                     /locus_tag="VGA49_02575"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX9664684.1"
                     /translation="MKKARNSLIFLVKKLENIGLGTKLIGSFFVCIILILLAVGLNFY
                     QFDQAQPALADFNWTITQVDTTNDVGQFTSLVLDSANNPRISAYDFTNRDLRYSFCSI
                     ASGCDSLVDWTSYTIDFSVNDVGAFSSLALDSANNPRIGYYDATDRDLKFTLCSIASG
                     CDEAGDWTTYIVDATGDSGAFASMALDANDNIGITYRHEEASAIKYAYCSIATGCDQS
                     GDWILGDPGDSGNFSSLAFDLTTGNPRIAHRNAFFGLRYAFCNDQALSGCDQATDWTI
                     YTVDNVGNLNVSLAMDSTQSPRIAYWHSPDNTLRFASCPIVSGCDEAGDWTITIADEV
                     AGNSVGQYASLILDSNDNPFISHYNQTIGDLRLSSCLISSGCDAPGDWNSENVDTGGD
                     VGLYTSIQVNADEPRISYYDVSNGDLKYAQTVNNPPDAPILVSPLNAATVISLTPQLY
                     ALYSDSDADDTGQVEFRVSSGSAADCLNNVNIVSSGASAESSTNLGPQFFTTDPLTNL
                     ATYNWCARNNDGTNTSAYADMGSFTVDTTVAAGLVYSSYLGGSSGEQVDDIIVDTAGN
                     AYVTGHTFSANFPTTTGAFDTSSNGDIDVFVSKIDANGDVLIYSTYLGGSNSEQETSQ
                     TNGLAVDSSNNVYITGSTESADFPTQNPFQAANAGGFDAFVTKLSADGSSLIYSTFLG
                     GSDVDNGFGIVVDISNQAYVTGETISNDFPTQNPFQANNAGFRNVFITKFSADGSSLD
                     YSTYLGGSNSESGFDIALDSSNNAYVTGRAESVDFPTQNPFQAVKGAGVDVFITKLSS
                     TGMSLVYSTYLGGSSQDVGNGIVVDSSNNAYITGETSSLDFPTQNPYQAVGTGGDVFI
                     TKLSSTGTSLVYSTYLGGNGSDLGNRIAIDSSNNAYVTGYTESTNFPTQDPYQAAFAG
                     GTGADVFVTKLSASGNSLSYSTYLGGTLNDIGYGIDIDSSGNIYVGGFTFSSNFPTAS
                     FQGAIGGVFDGFVSKLKPPTPPDDPVLISPLNGATVTSLAPQLYALYDHPEAANTGTV
                     EFRVSSGSAADCLNNVNIVSSGASSESSTETGPQFFTTAALTNLATYNWCARNNDGAD
                     TSAYATMGSFTVNTTVAEGLVYSSYLGGSGEDRATGIIVDSAGNAYIMG"
     gene            complement(3744..4319)
                     /gene="ruvA"
                     /locus_tag="VGA49_02580"
     CDS             complement(3744..4319)
                     /gene="ruvA"
                     /locus_tag="VGA49_02580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9664685.1"
                     /translation="MISYLSGIIIHKFSDSVLVDVNQIGYRVYLPADLLADLNMGDEA
                     KLFTHQYIRESSSELFGLISPDQLNFFELLLSISGIGPKSALGILSVAKVADIKKAIL
                     SEDASILTVVSGIGPKTAQRIILELKNKVKKEADQTGLEAFSSTDKEVIDGLVSLGYS
                     VQQARQAVSKLSVDSEDISDKLKEALKHLSK"
     gene            complement(4435..>5414)
                     /locus_tag="VGA49_02585"
     CDS             complement(4435..>5414)
                     /locus_tag="VGA49_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9664686.1"
                     /translation="NVYNFYLLYADRIKEQKVISPAGSASGTSDNFLDKWILSKLNLL
                     IKETTEVFDGYATAPAAEMIQNFFVNDLSTWYLRRSRDRIKGDNPAEQQAALQTLRTV
                     LLTLVKLMAPAIPFLTEEMYKTLGGQLESVHLEDWPVTDESLIHSQVNEAMDQVLKIV
                     ELVHAARAEAGVKLRQPLADLEISNGSRISGDQLDLIKEEVNVKEIILPVEIKEKNGY
                     LVKINEQIKIALNIELTDELKKEGLVREIVRQVNFLRKDRQLTITDQVELYYQTDDQE
                     VNEVFDKFNKKIQAETLSSKIISGIPAKAEGQNFKINGKTLKIGFLVKN"
BASE COUNT         1626 a         1193 c         1155 g         1440 t
ORIGIN      
        1 tggcccataa tataagcatt gccagctgaa tcaactatga tgcctgtagc ccgatcttcc
       61 ccactacctc ccaaataact ggaataaacc aatccttcag ccacggtcgt attaacagta
      121 aaactgccca ttgtcgcgta agcgctggta tcagctccat cattattccg ggcgcaccag
      181 ttgtaagtcg ctaaattagt cagagcggct gtcgtaaaaa attgcgggcc ggtttctgtt
      241 gatgattcac tgctggcccc gctggaaact atattgacat tattcagaca atcagcggcc
      301 gatcccgacg acactctaaa ctcaaccgtg ccggtgttgg ccgcttcggg atggtcatac
      361 aaagcgtaaa gttgtggggc taaactggtg accgtcgcgc cattaagcgg tgaaattaga
      421 accggatcat cgggcggggt gggcggtttt aatttgctga caaaaccatc aaagacccca
      481 ccaattgctc cctggaaact ggcggtgggg aaattagatg aaaatgtaaa cccaccaaca
      541 taaatgttac ctgaagaatc aatgtctatg ccatagccaa tatcattcag tgtgccgccc
      601 aaataggttg agtaactcaa gctattacct gatgccgaca gtttagtcac aaaaacatca
      661 gccccagtcc cgccagcaaa agcggcttga taagggtctt gagtcgggaa atttgttgac
      721 tctgtatacc ctgttacata agcattattt gaagaatcaa tagctatcct attacccaaa
      781 tcactgccgt tgccgcctaa ataagttgaa taaactaaac tcgtaccagt tgaagaaagt
      841 ttagttatga aaacatcgcc tccagttcct actgcctggt aagggttttg agttggaaaa
      901 tcaagtgaac tagtttctcc agtgatataa gcattattgg aagaatcaac cactatcccg
      961 ttgccaacat cctgggaact gccgcctaaa taagttgaat aaactaaact cataccagtg
     1021 gaagaaagtt tagttataaa aacatcgact ccagctcctt ttactgcctg aaaagggttt
     1081 tgggttggaa aatccactga ctctgcccgg ccagtcacat aagcattatt tgaagaatca
     1141 agggcaatgt caaacccact ctcggaattg ctccccccta gataagttga ataatctaaa
     1201 ctgctgccat cggcagagaa tttagttata aagacattcc tgaagccagc attattggct
     1261 tggaaaggat tttgtgttgg aaaatcatta gagattgttt cacctgtcac gtaagcctga
     1321 tttgatatat caacaacaat accgaaacca ttatccacat cactgcctcc taaaaatgtt
     1381 gagtaaatta aactactgcc atctgcggat aatttcgtca caaaagcgtc aaatccaccg
     1441 gcattcgcgg cctgaaaagg attttgcgtc gggaaatccg cagactccgt agagccggtg
     1501 atataaacgt tattagatga atcaaccgct aaaccattgg tctgactagt ctcctgctct
     1561 gaattgctgc cgcccagata ggttgaataa atcaaaacat cgccgttagc gtctatttta
     1621 gaaacaaaaa catcaatatc gccatttgag cttgtatcaa aagcgccagt tgttgtcggg
     1681 aaattcgctg aaaaagtatg ccctgtaaca taagcattac cagctgtatc aactataata
     1741 tcatcaactt gttccccact actccccccc aaatagcttg aataaaccaa cccggcggcc
     1801 acggtcgtat caaccgtgaa actccccata tcggcataag cgctggtatt agttccgtca
     1861 ttattccggg cgcaccagtt gtaagtcgct aaattggtca aaggatcagt cgtaaaaaat
     1921 tgcgggccta aatttgttga ggattcagcg ctggcgcctg atgagacgat attaacgtta
     1981 tttaaacagt cagcggctga cccggacgac accctgaatt caacttgacc ggtatcatca
     2041 gcgtcagaat cgctgtataa ggcgtagagt tgaggggtta aagaaatgac tgtggcggcg
     2101 ttcaatggtg aaaccagaat cggggcgtcc ggcggattat tgactgtctg ggcgtacttt
     2161 aaatcgccat tggaaacatc ataataactg atcctgggct cgtcagcgtt gacctgaatt
     2221 gaagtgtata gaccgacatc accgccagta tcaacatttt cagagttcca gtcgccgggg
     2281 gcgtcacagc cggatgaaat taaacaggaa gacagcctta aatcgccaat tgtctgatta
     2341 taatgactga taaaaggatt atcattggaa tcaaggatca gactggcgta ctggccgaca
     2401 gaattgccgg caacctcatc agcaatggtg atcgtccagt cgccggcttc atcacagcct
     2461 gaaactatcg ggcaggaagc aaaccgcaaa gtgttatccg ggctatgcca ataagcaatt
     2521 cggggacttt gggtactgtc catggccaga gaaacgttta aattgccaac attatcaacc
     2581 gtgtaaatag tccagtcagt cgcctgatca cagccggaca gagcctggtc attgcaaaaa
     2641 gcgtaccgca aaccaaagaa agcgttacga tgagctattc ggggattgcc ggtggttaaa
     2701 tcaaacgcca gtgaactgaa attaccacta tctccgggat cccccagaat ccagtcgcct
     2761 gactgatcac agcccgtggc aatggaacaa taagcgtact taatggcgct ggcttcttcg
     2821 tgacgataag taatgccaat attgtcatta gcgtccagag ccattgaagc aaacgccccg
     2881 ctgtcaccgg tggcgtcaac gatgtaagtc gtccagtccc cggcttcatc acagccggaa
     2941 gcgattgaac acaaagtaaa ttttaaatcc ctgtcagtgg cgtcataata accaatccgg
     3001 ggattattgg ctgaatccag cgccagagag ctgaaagcgc caacatcatt aactgaaaaa
     3061 tcaatcgtgt aactggtcca gtccactaaa gagtcacagc cggaagcaat agaacagaaa
     3121 gaatatctca aatcccggtt ggtaaaatca taagcgctga tccggggatt attggcgctg
     3181 tccaggacta aagaagtaaa ctggccgaca tcattggttg tgtccacttg agtaattgtc
     3241 cagttaaagt ccgccagagc cggctgagcc tgatcaaact gataaaaatt aaggccgacg
     3301 gccagtaaaa ttaaaataat acaaacaaaa aaagaaccaa ttaatttagt ccctaagcca
     3361 atgttttcta attttttaac taaaaaaatt aaactgttcc gtgctttttt catttaggtc
     3421 gcgaactcgt taaaaaggtt ttattttgct gaaataatta taccatttta tttctaaaag
     3481 gcaaagaaaa ggtgtggata aacaagcagg ggagatgaag ggggataaag ggagataaag
     3541 ggagataaag ggagataaag ggagatgaag ggagatgagg ggagatgaag gttaaaagat
     3601 caaagttcaa agtccaaagc tcaaatcaag ctcaaagctc aaaatctaaa taatttatca
     3661 tttgtcattt ggatttgaat tgatatttga gctttgatat ttggatttta tagctggatt
     3721 ttgaaatttt tattattgct tctctattta gataaatgct tcagggcctc tttgagttta
     3781 tctgaaatat cttctgaatc aacagaaagc ttagagaccg cctgccgggc ttgctggact
     3841 gaatagccca gactgaccag cccgtcaatc acttctttat cggttgaact gaaggcttcc
     3901 agcccggtct ggtcagcttc ttttttaact ttatttttca gttccaaaat aattctttgg
     3961 gcggttttgg ggccaatccc cgagactacc gttaaaatac tggcgtcttc gctcaaaatc
     4021 gcttttttaa tgtcggccac tttagcgact gacaaaattc ccagagctga ttttggtcca
     4081 atgccgctga tgcttagtaa gagttcaaaa aaattaagct ggtcaggact gattaaacca
     4141 aacagctcac tggagctttc cctgatatat tggtgggtga aaagtttagc ctcgtctccc
     4201 atattcaaat cagccaatag atcagccggg agatagaccc ggtaaccgat ctgattgaca
     4261 tcaaccagaa ctgaatcaga aaatttatga atgattattc cggagagata agaaatcatg
     4321 gctaagaggg ttaaggggga atgagaggga atcagaagga atcagaagga atcaaaagga
     4381 agcaaaggga atcagaagga atgagaggaa atgagaggga taaggagtgt tggcttaatt
     4441 ctttaccagg aaacctattt ttaaagtttt gccgttaatt ttaaagtttt gcccttcagc
     4501 tttagccgga attcctgaaa ttatctttga cgataaagtt tcagcctgaa tttttttatt
     4561 aaatttatca aaaacttcat taacctcctg atcatctgtc tgataataca gttcaacctg
     4621 atcagtaatc gtcagttgcc ggtcttttct taaaaaattt acctgacgga caatttccct
     4681 gaccagtcct tcttttttca gctcgtcagt cagttcaata ttaagagcaa ttttaatctg
     4741 ttcattgatt tttaccagat aaccattttt ctcttttatt tcaaccggca aaataatctc
     4801 tttgacatta acctcctctt taattaagtc cagctggtcg ccgctgattc tggagccgtt
     4861 gctaatttcc aggtcagcca gcggctggcg gagcttaacc ccggcttcag cccgggcggc
     4921 gtgaaccagc tcaacaattt tcaaaacctg atccatggct tcattaacct gggaatgaat
     4981 taaagactcg tcagtcactg gccagtcttc cagatgaact gactccagtt gacctcccaa
     5041 agttttatac atttcttccg ttaaaaacgg aatggccggg gccattaatt taaccagagt
     5101 cagtaaaaca gttcgcaaag tttgtaaagc ggcctgttgt tcagctggat tgtcgccttt
     5161 aattcggtcg cggctccttc ttaaatacca ggttgacaaa tcattgacaa aaaaattctg
     5221 aatcatttca gcggccggcg ctgtcgcgta gccgtcaaaa acttcagttg tctctttaat
     5281 taacaaatta agttttgata aaatccactt atccaaaaaa ttatcgcttg tcccgctggc
     5341 gctgccagcg gggctgatga ctttttgctc tttgatccgg tcagcgtaga gcaaataaaa
     5401 attatagaca ttcc
//
LOCUS       DASWHZ010000122         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_618005, whole genome shotgun sequence.
ACCESSION   DASWHZ010000122 DASWHZ010000000
VERSION     DASWHZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618005"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..346
                     /gene="rplM"
                     /locus_tag="VGA49_02590"
     CDS             17..346
                     /gene="rplM"
                     /locus_tag="VGA49_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9664687.1"
                     /translation="MDATDKVLGRLASEIAVILRGKNKPIFAPNKDIGDFVNVENAGK
                     LKITGNKLEQKKYFSHSGYPGGLKEKTMGEIFAKDPGEILRKAVYKMLPGNKLRNNMM
                     KRLKIIN"
     gene            353..814
                     /gene="rpsI"
                     /locus_tag="VGA49_02595"
     CDS             353..814
                     /gene="rpsI"
                     /locus_tag="VGA49_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9664688.1"
                     /translation="MAKEVDTTKEKEAQQPETAEKFGKGKYFYAVGKRKSAIARVRLF
                     KAGSGQIMVNSKKFEEYFPTEYLQNIVLSPLSLVSRVKDHDVEVKVIGGGTRGQADSV
                     RHGIAQALLLFDVDLRKTVKKAGFLTRDARVKERKKYGLKKARRAPQWQKR"
     gene            888..>1606
                     /locus_tag="VGA49_02600"
     CDS             888..>1606
                     /locus_tag="VGA49_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX9664689.1"
                     /translation="MANPKSIAQNTTYLTISFVFQKILSFIYFTLIARLIGVEGTGIY
                     VFAISYTTIFSVFIDLGLSPVLTRESARNKEQSEKYLANILGLKIILSFFILLAVLTS
                     INLVGKPPLTRLMVYLASALMVLDSFTLSFWALFRGWQNFFYEAKSVVFTQIIIVTVG
                     ITGILLKFPLYVLVIALISGSVFNFSYSLILLKKNLTIKPRLEFDKKTVRFLLTIAIP
                     FALAGIFTRFYSYLDQVLLSF"
BASE COUNT          478 a          285 c          340 g          503 t
ORIGIN      
        1 cagacaagtc cacaaaattg acgccactga taaagttctg ggccggctgg cttcagagat
       61 cgcggttatt cttcggggaa agaataagcc gatctttgct cccaataaag atattggcga
      121 ttttgttaat gttgagaacg ccggcaaact taaaattacc ggcaataaac tggagcagaa
      181 aaaatatttc agtcattccg gctatcccgg agggttaaaa gaaaaaacaa tgggtgaaat
      241 ttttgccaaa gatcccggag agattttaag aaaagcggtt tataaaatgc tgcctggaaa
      301 taaattaaga aacaacatga tgaagaggct aaaaattatt aattaaggat atatggctaa
      361 agaagtggat acaactaaag aaaaagaagc tcaacagcct gaaacagctg aaaaattcgg
      421 taaaggtaaa tatttttatg ctgttggcaa aagaaaatca gccattgccc gcgttagatt
      481 atttaaagcc ggttcaggtc agattatggt taattccaaa aaatttgaag aatattttcc
      541 aacagaatat ttgcaaaata ttgtcttgtc tcctttaagt ctggtcagcc gggttaaaga
      601 tcatgatgtg gaagttaaag ttatcggcgg cggcaccaga ggacaggcag acagcgttcg
      661 gcacggaatt gcccaggccc ttctcctgtt tgacgtggat ttaagaaaaa cggttaaaaa
      721 agcaggcttt ttgaccagag acgcccgggt caaggaaaga aagaagtacg gtttaaagaa
      781 agcccgtcgg gccccgcaat ggcagaaacg ataatcattt tgttactatt taaaaagact
      841 ttccagaagt ctttttaaat ttttgattta aagtataatg taacaagatg gccaatccta
      901 agtctatcgc ccaaaatacg acttatttga cgatatcttt tgtttttcaa aagattctgt
      961 cttttattta ctttactttg atcgcccggt tgattggggt agaagggacg ggcatttatg
     1021 tttttgccat ttcctacacg actatttttt cagtttttat cgatctgggg ttaagcccgg
     1081 tgttaaccag ggagtcagcc cgaaacaagg aacagtcaga aaaatattta gccaatattc
     1141 ttggtctgaa aattattctg tcttttttta ttttactggc ggttttgact tcaattaatt
     1201 tggtcggcaa gccgcctttg accagattaa tggtttactt agcttcagcg ctgatggttc
     1261 tggattcatt tactttgtcg ttttgggcgc tgtttcgggg ctggcagaat tttttctatg
     1321 aagccaaatc agttgtattt acgcagataa ttattgtaac ggtcggtatc accggtattt
     1381 tactaaagtt cccactttat gttttggtga ttgctttgat cagcggcagt gtttttaatt
     1441 tcagttactc tttgattctt ttaaagaaaa atctgactat caaacccagg cttgagtttg
     1501 acaaaaaaac cgtcaggttt ttgttaacga tcgccattcc ctttgccctg gccggtattt
     1561 ttaccagatt ttattcctat ctagaccagg tcctgctgtc tttttt
//
LOCUS       DASWHZ010000123         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3217637, whole genome shotgun sequence.
ACCESSION   DASWHZ010000123 DASWHZ010000000
VERSION     DASWHZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217637"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGA49_02605"
     CDS             complement(<1..518)
                     /locus_tag="VGA49_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9664690.1"
                     /translation="MEKSYLDYAMSVIVSRALPDVRDGLKPVHRRILYAMWSVGLKAG
                     SKFKKSATVVGEVLGKYHPHGDIAVYDTMVRMAQDFSMRYPLVWGQGNFGSMDGDAAA
                     AMRYTEAKLTPIAEELLYDLDKNTVDFIPNYDGSQHEPGVLPARLPNLLLNGAVGIAV
                     GMATNIAPHNLN"
     gene            complement(649..1365)
                     /locus_tag="VGA49_02610"
     CDS             complement(649..1365)
                     /locus_tag="VGA49_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664691.1"
                     /translation="MKKDHLQQKGILVWILVIGSMVVILILWFFYFKNYLDFMKSDTV
                     TSAGQTGGTADFQKLLDQLSANIDDFKSKIETNLEDQQKLNQVNQQVAAALLADVSWS
                     RHQDQKLGLAIDFPDDWAVRTDENQIGFYPQSIADKLSPNINEDLVNIKITPDIKEET
                     LTGWFNNNFSNIEKTDLEIDGWPAIQLVASLGNQSVINTYLLVKNSLYNVTFLEYKTV
                     DEQAKNIYTKMITSIQFLKP"
BASE COUNT          367 a          342 c          360 g          449 t
ORIGIN      
        1 tcatttaaat tatgcggggc aatgttggtc gccatgccaa ccgcgatgcc gaccgctccg
       61 ttgagcagca gattgggcag gcgggccggc aggacgcccg gttcgtgctg ggagccgtcg
      121 tagttgggaa taaaatcaac cgtgtttttg tccagatcat aaagcagctc ttcggcaatc
      181 ggcgtcagtt tggcttcagt gtaccgcatg gcggccgccg cgtcgccgtc catggagccg
      241 aagttgccct ggccccagac caaaggatac ctcatcgaaa aatcctgggc catccggacc
      301 atcgtgtcgt aaacggcgat atcgccgtga ggatgatatt tacccaaaac ttcaccaaca
      361 accgtggcgg attttttaaa ttttgagccg gctttcaggc caacgctcca catagcgtaa
      421 agaatccggc gatggaccgg tttcaggccg tcgcggacat caggcaaagc ccgggagaca
      481 atgactgaca tggcgtagtc cagataggac ttttccattt ccttaaccag ggaatgttca
      541 attattttac caacccgttc ctttgtggtc tggtcagctt ctttttggtc agtcgccggg
      601 ttctttttaa ccggctggtc ttttttattg ggagtagatt tagccatatt aaggctttaa
      661 aaattgtatg ctggttatca tcttagtata gatgttttta gcctgttcgt caactgtttt
      721 gtattccaaa aaagtaacat tatacaggct gttttttaca agcaagtaag tgttaataac
      781 cgactgattg cccaggctgg ctaccagctg aatagccggc caaccgtcaa tttccagatc
      841 tgttttttca atattgctga aattattatt gaaccagccg gtcagggttt cttccttaat
      901 atccggagta atttttatgt taaccaggtc ttcattaata ttcggcgaca gcttgtcagc
      961 gattgattga ggataaaaac caatttggtt ttcatctgtc cggacggccc agtcatcggg
     1021 aaagtcaatc gccaaaccca gtttttgatc ctgatggcgg gaccagctga cgtcggccag
     1081 caaagcggcc gctacctgct gattgacctg attaagtttt tgctggtctt ctaaatttgt
     1141 ttctattttt gatttgaaat catcaatatt agcgcttaac tggtcgagta gtttctggaa
     1201 gtcggctgtc ccgccggtct gcccggcgct ggtgacggtg tcggatttca taaaatcaag
     1261 atagttttta aaataaaaaa accacagaat cagaatgacg accatactgc cgataaccag
     1321 aatccagacc aggatacctt tttgttggag atgatctttt ttcatgtttg atgagctgga
     1381 tctaaaatca ggctaatgtt ggatttattt tgccagcaaa atgacctggg tttcttcggt
     1441 cggcaaatct ttcctggtga ttttgtattt atcaagggtt tcagacgggg aacagcccat
     1501 ttctttgcaa aacttgtc
//
LOCUS       DASWHZ010000124         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1742929, whole genome shotgun sequence.
ACCESSION   DASWHZ010000124 DASWHZ010000000
VERSION     DASWHZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742929"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VGA49_02615"
     CDS             complement(<1..624)
                     /locus_tag="VGA49_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664692.1"
                     /translation="MRLFKLQIIDYSFYSALASDQHEIYLTLVPKRGEILVADYNSDQ
                     LYPLATSRDFNFIFGIPVEIDNPAETTEILTELLRLDEEEAELLLVKLSKENDPYEPI
                     KHKVTDEIVEQIKEKELTGIRFVKESWRYYPMESLSSQLIGFVGFEGDQQRGLYGLEG
                     YFDEELSGQPGVLETERDVAGRWIALTEKKVEKAEDGSDIILTIDRTV"
     gene            complement(736..1041)
                     /locus_tag="VGA49_02620"
     CDS             complement(736..1041)
                     /locus_tag="VGA49_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664693.1"
                     /translation="MSKFSKLRSTSKTPYIFIMMAVVLVGLVYIVQTNGTATKGYTIM
                     SLEERLNQLNEENKQLKMEATELQSLSRIEKIIGVYEMTTVAQIDYLSPAGSVVAVK"
     gene            complement(1056..>1616)
                     /gene="rsmH"
                     /locus_tag="VGA49_02625"
     CDS             complement(1056..>1616)
                     /gene="rsmH"
                     /locus_tag="VGA49_02625"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX9664694.1"
                     /translation="GQDQKISQVKAHKIINYFTEQELAAIFRDYGEEKYARAIARKIV
                     QVRKIAPIKTTTELVELIKKAVPAGYLHRRIHPATRVFQALRIAVNQELENLKEILPA
                     AVDILEPGGRLVVIAYHSLEDRIVKRFFRQEAKDCLCPPQLPDCRCDHQAQIKILTPR
                     VVKPGREEILKNPRSRSAKLRAVEKI"
BASE COUNT          443 a          372 c          297 g          504 t
ORIGIN      
        1 gacagtccgg tcaattgtta aaataatatc tgaaccgtct tcagcttttt caactttttt
       61 ttcagtcaga gcgatccagc ggccggcgac atcccgctct gtttctaaaa cgcccggctg
      121 gccggatagt tcctcgtcaa aataaccctc caaaccatat aagcctcttt gttgatcgcc
      181 ttcaaaacca acaaaaccaa tcagctgact gcttaaactc tccattggat aatatcgcca
      241 gctttcttta acaaatctta ttccagtcaa ctctttttcc ttaatttgct caactatttc
      301 atcagtgact ttatgtttaa tcggctcata aggatcgttt tctttactaa gcttaactaa
      361 caaaagttca gcctcttctt cgtctaatcg caaaagctct gtcaaaattt cagtggtttc
      421 ggccggattg tcaatttcaa ccggaatacc gaatataaaa ttaaaatccc ggctggtggc
      481 caaaggatat agctggtctg aattgtaatc ggctaccaga atctcacccc ttttgggaac
      541 taatgtaaga tagatttcgt gctgatcgga agccagcgcc gaataaaaag agtaatcaat
      601 tatctgcaat ttaaaaagac gcaaaattat caggccgccc agaataaaaa taaataaaag
      661 cagtaaattt aaacggtcca gctcaaataa tctatttttt atttttttaa atgcctctga
      721 cagatcttta tgactttatt taacggcgac gaccgaacca gctggcgaca gataatcaat
      781 ttgagcgact gttgtcattt cataaacacc gattattttt tcaatccggc tcaatgactg
      841 cagttcagtc gcttccattt ttaattgttt attttcctcg ttcagctgat ttaaccgctc
      901 ttccagactc attatagtat accctttggt ggctgtcccg ttagtctgaa caatatagac
      961 taaaccgacc aaaaccaccg ccatcattat aaaaatatag ggggtttttg aagttgaacg
     1021 aagtttggaa aactttgaca tttttgtgtt tatttttata ttttttccac tgctcttaat
     1081 ttggcgctcc gtgatctggg attttttaaa atctcctccc ggccgggctt aacgaccctg
     1141 ggggtcaaaa tttttatctg agcctgatgg tcacaacggc aatctggcag ctgcggcgga
     1201 caaaggcagt ctttggcttc ttgtctgaaa aaccgcttaa caatccggtc ttccaaagaa
     1261 tggtaagcga ttaccaccag ccggcctccc ggttccagaa tatcgacagc cgccggcaga
     1321 atttctttca aattttccag ttcctggttg acggcaattc tcaaagcctg aaaaaccctg
     1381 gtggccggat gaatccggcg atggagatag ccggccggaa cagccttttt aatcaattca
     1441 accaattccg tggttgtttt aatcggggca atttttctga cctgaacaat ttttctggcg
     1501 attgcacggg cgtatttttc ctcgccatag tctctgaata tcgcggccag ttcttgttca
     1561 gtaaaataat taataatctt atgagctttg acttgggaaa tcttttgatc ctgacc
//
LOCUS       DASWHZ010000125         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3555140, whole genome shotgun sequence.
ACCESSION   DASWHZ010000125 DASWHZ010000000
VERSION     DASWHZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555140"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1118)
                     /gene="pheS"
                     /locus_tag="VGA49_02630"
     CDS             complement(96..1118)
                     /gene="pheS"
                     /locus_tag="VGA49_02630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9664695.1"
                     /translation="MIMLKELTKIKEQATQEIQAVQNEKDLDQVEIRYLGRKAGQLTL
                     ILKKIKDLSAAEKKTVGHQANQLKADLEFLIKNKKLELKDQLDLEKEWLDVTLPGQVS
                     QAVGHLHPNSRIQYELEDLFRSMGFMVLDGPELESEYYNFEALNTPASHPARDSQDTF
                     WLSDGNLLRTQTSAVQVRALREYGAPLRAIVPGRCFRYEQTDASHENTFYQLEGLMVD
                     KNISIANLIATMEALLEGVFKQDLDVRLRPGYFPFVEPGFELDMNCLICSGQGCSFCK
                     QSGWVETLPCGLVHPNVLKAGGLDPKKWSGFAFGLGLTRLVMMRYGIDDIRLMNSGDL
                     RFLQQF"
     gene            1191..>1607
                     /gene="trpS"
                     /locus_tag="VGA49_02635"
     CDS             1191..>1607
                     /gene="trpS"
                     /locus_tag="VGA49_02635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9664696.1"
                     /translation="MAKKQTIFSGVGPSGNLHLGNYLGAIKNWLELQNQYNCIFCVVD
                     HHAITVKQKPTVLREKILEVAKIYLACGIDPKKAIIFIQSHVKEHTELAWILGTLTKV
                     AEMERMTQYKDKISRKKSANIGLLSYPILMACDILLY"
BASE COUNT          450 a          395 c          316 g          446 t
ORIGIN      
        1 ttgagctttg atatttggat ttaatagctg gattttgcct gcctgccgca ggcaggaaat
       61 ttttatcctt gcttcccctt gcttcctttt atttttcaaa attgttgcag aaaccgcaaa
      121 tccccgctat tcatcaatct aatatcatca atcccgtagc gcatcatcac cagccgggtt
      181 agtcccaggc caaatgcaaa gcccgaccac ttcttgggat ccaggccgcc ggctttcaaa
      241 acattgggat ggaccaggcc gcagggcaat gtttccaccc agccggactg tttgcaaaaa
      301 ctgcagcctt ggcccgaaca aatcagacag ttcatgtcca gttcaaagcc cggctcaaca
      361 aaagggaaat atcccggccg taatctgaca tccaggtcct gtttaaaaac gccttccagc
      421 aaagcttcca tggtagcgat taaattggcg attgaaatat ttttatcaac cattaaacct
      481 tccagctgat aaaatgtatt ttcatgagaa gcgtcagtct gctcgtaacg aaaacagcgc
      541 ccgggcacga tcgcccggag cggcgctcca tattctcgca aagctctgac ctgaacggct
      601 gaagtctggg ttcttaataa attgccgtca gacagccaga aagtgtcctg gctgtcccgg
      661 gccgggtgac tggccggcgt gttcaaagct tcaaaattat aatattcaga ttccagttca
      721 ggcccgtcca aaaccataaa gcccattgat ctgaacaaat cttccagttc gtactggatt
      781 ctggaattag ggtgcaaatg accaaccgcc tgactaacct ggccgggcaa tgtcacgtcc
      841 agccattctt tttccagatc aagctggtct ttcagttcca gttttttgtt tttaattaaa
      901 aattccaaat cagcttttaa ctgattagcc tggtgtccaa ccgtcttttt ttcagcggcc
      961 gataaatctt tgatcttttt caaaatcaaa gtcaactgcc cggcttttcg gcccaggtaa
     1021 cggatttcaa cctgatccaa atctttctca ttttgaacag cctgaatctc ttgagtagct
     1081 tgttctttaa ttttggttag ctcttttaac attatcaaat tataacaaaa gaaccgcgct
     1141 ttgacaaagg ccttaataca agataaaatt taataaaata acctatcttt atggcaaaga
     1201 aacagaccat cttctccggc gtcgggcctt caggcaatct tcatctcggc aactatttag
     1261 gcgccattaa aaactggctg gaattgcaaa atcagtacaa ctgtattttt tgcgtggttg
     1321 atcatcacgc cattaccgtc aaacaaaaac caacggttct ccgcgaaaaa attctggaag
     1381 tggccaaaat ttatctggct tgcgggattg atcccaaaaa agctatcatc tttatccagt
     1441 cgcatgtcaa agaacatacg gaactggcct ggattttggg gactttaacc aaagtggcgg
     1501 aaatggaacg aatgacccag tacaaagaca aaatcagccg gaaaaaatca gccaatatcg
     1561 gcctgctgtc ctatccaatt ttgatggcct gtgatatttt actgtat
//
LOCUS       DASWHZ010000126         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3219591, whole genome shotgun sequence.
ACCESSION   DASWHZ010000126 DASWHZ010000000
VERSION     DASWHZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3219591"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGA49_02640"
     CDS             <1..517
                     /locus_tag="VGA49_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664697.1"
                     /translation="YTGLIGSDNNAMAALARSTGLSPEEFADLMNAKADSLKMNNSHF
                     IEPTGLSPLNVSTARDVLKLARAAFADEKISEILRIKKYQFTSASNISHTVYSTDLLL
                     NSFLNADPYQVVAGKTGFINEAGYCLTAQISHSEQNGEILAVVLGTTGHLNRFDEMKG
                     LVSWVFDNYEF"
     gene            522..1010
                     /locus_tag="VGA49_02645"
     CDS             522..1010
                     /locus_tag="VGA49_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEX9664698.1"
                     /translation="MNGLIELFAGLPRELATMLTAMLPIGELRLSIPVALGVYKLSIF
                     SSYFFAVIGNIIPVIFLLLFLEPGSRWLIQNSKIFNRFFSWLFARTRDKHLEKFELLG
                     AIALITLVALPVPLTGAWTGTVAAFVFGVPFWRALLYISVGVGIAGFIVTLASLGFFA
                     II"
     gene            1066..1791
                     /gene="tpiA"
                     /locus_tag="VGA49_02650"
     CDS             1066..1791
                     /gene="tpiA"
                     /locus_tag="VGA49_02650"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9664699.1"
                     /translation="MNFRKIIIGNWKMYLSLKESLRLAKDLKKIKLKKGVEVVVCPSL
                     SALTEVKKVLPKKIKLGAQNVFWEKKGAYTGEVSAGLLKELGCHYVIVGHSERRQYLE
                     ETDEMINQKVLAVLENKLVPIVCVGENLAQRRKKQTEKVILNQLEKNLKNVKIQNADK
                     LIIAYEPIWAIGTGQACRPEEAVRIHNLIRQRWPGTKIIYGGSLDGGNIASYSVQPEI
                     DGALVGGASARAKEFIKLLNNVI"
     gene            1781..2665
                     /locus_tag="VGA49_02655"
     CDS             1781..2665
                     /locus_tag="VGA49_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9664700.1"
                     /translation="MSYNIIPFIIIILSLAGIFIIVIKKFPHLLAIDVGSIPAEKEKQ
                     KKEEIIKKRANRIISGWLSLIIPKIRPLATGIKDILKDFYRKILALEKFYQKKTALSQ
                     EEKKDLDSKIRQMLQETDELIKEENYNEAEKKYIEIISWDSKNTGVYKKLADLYYEQK
                     EYKQAKETLEYLIKLDSADAEIYDRLGHIAREQGNLAEAKKDYKKSIALNDKTAHYHF
                     DLGEINLVMGDYQAALENLKKATDLESNNPKYLDKLIEIYILLKERGQALDTLKTLKG
                     VNPENQKIADFEERIRAI"
     gene            2697..3635
                     /gene="glpX"
                     /locus_tag="VGA49_02660"
     CDS             2697..3635
                     /gene="glpX"
                     /locus_tag="VGA49_02660"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX9664701.1"
                     /translation="MDRNLALEFVRVTEVAAIAAAKWIGRGDRRAADQAAVDEMRSRF
                     NDIDFSGRIVIGEGKKDEAPELYVGEVVGQRSGLEVDLAVDPLECTDSVANGRPNAIS
                     VIASAEKGAMRVGPDTYMKKIAVGPAAAGKVDLLAPVADNLKNIARALDKKVGELKII
                     ALDRAYNEQIILAIRQAGARVELITDGDVAGAIAPSIPDSGIDALMGIGASSEGILAA
                     AALKALGGDIQIQWNLFKHPELAKDLLEAGWSLDQVYSRDDLIKTDLVTFTATGICSG
                     PILKGVSFGADTIKTHSVVMRGKTKTIRWIETEHQA"
BASE COUNT         1100 a          684 c          900 g         1014 t
ORIGIN      
        1 ttataccggg ctaatcggtt cggataacaa cgccatggcc gctttggccc ggtcaaccgg
       61 tttgagccct gaagagtttg ctgatttaat gaacgccaaa gctgacagtt taaaaatgaa
      121 caactcacat tttatcgagc caaccgggtt gagtcctctg aatgtttcaa ccgcccggga
      181 tgttttaaag ctggcccggg cggcttttgc tgacgaaaaa atttctgaaa tattgagaat
      241 taaaaaatat cagtttacgt cagccagcaa tatcagccat acagtttaca gcaccgatct
      301 ccttttaaac agttttttaa acgccgatcc ttatcaggtg gtagccggca aaaccggttt
      361 tattaacgag gcgggctatt gtctgaccgc tcaaatcagc cactctgaac aaaacggcga
      421 aattttggcg gttgttttgg gaactaccgg ccatttaaac cggtttgatg aaatgaaagg
      481 cctggtcagc tgggtgtttg ataattatga gttttaattt tatgaatggt ttaattgaat
      541 tatttgcggg cttgcccagg gaactggcca ccatgctgac ggcgatgctg ccgattggcg
      601 agttaaggct gtcaatcccg gtcgcgctgg gagtttataa actatccatt ttcagcagtt
      661 atttttttgc agtaatcggc aatattattc cggtaatttt tcttctgttg tttttggagc
      721 ccggctcgcg ctggttaatt caaaattcaa aaatatttaa tcgttttttt agctggttat
      781 ttgccagaac cagagacaag catctggaaa aatttgagct actgggagcc attgctttaa
      841 ttaccctggt ggccttaccg gtgccgttga caggcgcctg gaccgggacg gtggccgcct
      901 ttgtctttgg ggtgcctttt tggcgggcgt tattatatat ttcagtcggc gtcggtatcg
      961 ccggatttat agtgacgctg gctagtttgg ggtttttcgc gataatttag ggtatagggg
     1021 atgggttata gggtatagtg aattgtttaa gtataaaaaa caaatatgaa ttttagaaaa
     1081 atcattattg gtaactggaa aatgtatctt tctttgaaag agagtttgcg attggcaaaa
     1141 gatttgaaaa aaattaaatt gaaaaaagga gttgaggtag ttgtctgccc gtctttgtcc
     1201 gctttaaccg aagttaaaaa agttctgcct aaaaagataa aacttggcgc tcaaaatgtt
     1261 ttttgggaga aaaaaggcgc ttatacgggt gaagtttcag cgggcttact gaaagaactg
     1321 ggctgccact atgtaattgt tggtcattct gaaagacgac aatatcttga agagacagat
     1381 gaaatgatta atcaaaaagt tttggctgtt ttagaaaata agctggttcc cattgtctgt
     1441 gttggtgaaa atctggccca gaggagaaaa aaacagactg aaaaagttat tttaaatcaa
     1501 cttgaaaaaa atctgaaaaa tgttaaaatc cagaatgctg ataaattaat cattgcttat
     1561 gagccaattt gggcgattgg caccggccag gcctgccggc ctgaagaagc tgtcagaata
     1621 cataatttga tcagacagcg ctggcccggg actaaaatta tttatggcgg cagtctggac
     1681 ggcggcaata tagccagtta tagtgttcag ccggagattg acggcgctct ggttggcggc
     1741 gccagcgccc gggctaagga gtttattaaa cttttaaaca atgtcatata atatcattcc
     1801 attcataatt atcattctaa gtctggccgg catttttatt attgttataa aaaaatttcc
     1861 tcatctgctg gccatagatg ttggatcaat tccggccgaa aaagaaaaac aaaaaaaaga
     1921 agaaatcatt aaaaaaagag ccaaccggat tattagcggc tggctaagcc tgattattcc
     1981 taaaattcgt ccgctggcga ctggcattaa agatattctc aaagattttt acaggaaaat
     2041 tttggcgctg gaaaagtttt accagaagaa aaccgccctg agtcaggaag aaaaaaagga
     2101 tctggattct aaaatcaggc aaatgctgca ggagactgac gaattaatta aagaagagaa
     2161 ttataatgaa gcagaaaaaa aatatattga aattatcagc tgggacagta aaaataccgg
     2221 agtttataag aaattagccg acctttacta cgagcaaaag gaatataaac aggccaaaga
     2281 aactttggaa tatttaatta agcttgattc ggctgacgcc gaaatttatg atcgattggg
     2341 ccatattgcc cgcgaacaag gcaatctggc tgaagccaaa aaagattata aaaaatcaat
     2401 tgccctcaat gataaaacag cccattatca ttttgattta ggtgaaatta atttggttat
     2461 gggcgattat caggccgcac tggaaaattt aaaaaaagcg actgatttag aatcaaataa
     2521 tcccaaatat ctggacaagc tgattgaaat ttatattctt ttaaaagaac gcggccaggc
     2581 cctggatact ttgaaaaccc tgaagggggt taatccggaa aatcaaaaaa tagccgactt
     2641 cgaggaaaga atcagagcta tctaatagtc gtcactaact accaactacc aatctcatgg
     2701 atcgtaattt agctttggaa tttgtcaggg tgacagaagt ggcggcgatc gcggccgcta
     2761 aatggattgg ccggggcgac cggcgggcgg ctgatcaggc ggcggttgac gagatgcggt
     2821 ccaggtttaa tgacattgat ttttcaggca gaatagtcat tggggagggc aaaaaagatg
     2881 aagcccctga actttatgta ggcgaagttg tcggtcagcg gagcggtttg gaagttgatt
     2941 tggcggttga tcctttggag tgtactgaca gcgtggccaa cggccggccc aacgctattt
     3001 cagtcattgc ttcggccgaa aaaggagcca tgagagtcgg accagacact tatatgaaaa
     3061 aaatcgctgt cggtccggcg gctgccggta aagttgattt gttggcgcct gttgccgata
     3121 atttgaaaaa tattgcccgg gctctggaca aaaaagttgg cgagttaaaa atcattgctc
     3181 tggaccgggc ttataatgaa caaataattt tggcaatccg ccaggccggc gcccgggtgg
     3241 aactgattac tgacggcgat gtcgccgggg cgattgctcc cagcattcct gactcgggca
     3301 ttgacgcctt aatgggtatt ggagcttcat cagagggaat tttagcggcg gccgcgctta
     3361 aagctctggg cggtgatatt cagattcagt ggaatttatt taagcatccg gaactggcca
     3421 aagatttact tgaagccggc tggtcgctgg atcaggttta cagccgggat gatttgatta
     3481 aaactgattt ggtgacattt acggccactg gcatttgctc cggaccaatt ctcaaagggg
     3541 tcagttttgg ggcggatacc atcaaaactc attcggtcgt catgcgcgga aaaactaaaa
     3601 ctatcaggtg gatagaaacc gagcaccaag cttgaaaata tgtcagattt atattcaaaa
     3661 attcaaacaa ttctggattc ggacgctgaa tacttgtt
//
LOCUS       DASWHZ010000127         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_620870, whole genome shotgun sequence.
ACCESSION   DASWHZ010000127 DASWHZ010000000
VERSION     DASWHZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620870"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VGA49_02665"
     CDS             complement(<1..1343)
                     /locus_tag="VGA49_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664702.1"
                     /translation="MLSPKFKKLLYIFLTLFGISWSSFIIFKYLTARSHYNFLHSIGQ
                     LYPTALLVLALTLLPALGWFFYLKFRKKKFNLDNFKIYYVIVAGLIIFLILGNILYLK
                     KDIGTYTGPTVTRSGNELIINGQDKSAPIILNKGDLTYNIKNRFANYPEIHNNFQRPS
                     FFARQTALIKNFLFFNLVILIFGCLSFVLGKRFLSWLKIEFGPLSEFVIASALGLMTI
                     SFLSWWLALLHLFNFWLVWLLIIAGLISHYKYLTELFKLVFIKKFKISLNFWTIISFG
                     FILIFINLNNLDLIKPFPVGWDEIQLYSNLPKLLADYGYLVPQVGLQAWTLVTGHGFL
                     LFKSLTAAYALTHFAFLLLLGGLFIFLKQFTSSKYALLLTTFFSGLPFVFFQTTQDVK
                     VDLPAALFMVISLILFFQWLEHSENQSKIINKYLYLSVLLVGFAFSVKVTAIAFV"
     gene            complement(1441..>1637)
                     /locus_tag="VGA49_02670"
     CDS             complement(1441..>1637)
                     /locus_tag="VGA49_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9664703.1"
                     /translation="YATDLLINGADIRSVQAMLGHASITTTQIYTHITNQQLRDVHKA
                     FHGRKRKQQESKGSTGEKKE"
BASE COUNT          546 a          280 c          354 g          457 t
ORIGIN      
        1 aagacaaagg caatggccgt tactttgaca gaaaaagcaa aaccgaccag caggactgac
       61 aagtaaagat atttattgat aattttagat tgattttcag aatgttccag ccactggaaa
      121 aataaaatca atgaaataac cataaaaaga gcggctggca gatcaacttt gacatcctgg
      181 gtcgtctgaa aaaagacaaa cggcagaccg ctgaaaaagg ttgtcagcag cagggcgtat
      241 ttagaagagg tgaactgttt tagaaaaatg aacaggccgc ccaggagtaa taaaaaggca
      301 aaatgagtca gggcgtaagc ggctgtcagc gatttaaata gtaaaaatcc atggccggtc
      361 accagcgtcc aggcctgaag cccgacttgg ggaaccagat agccataatc agccagcagt
      421 tttggcaggt tggagtaaag ctgaatttca tcccagccga cagggaaggg tttgattaaa
      481 tcaagattat ttaaattaat aaaaattaaa ataaacccaa agctgataat cgtccagaaa
      541 ttcaaagaaa ttttaaattt ttttataaaa actaatttaa aaagttcagt taaatacttg
      601 tagtggctga ttaagccggc aataattaac agccagacca gccagaaatt aaataaatgg
      661 agcaaggcca gccaccagct taaaaaagaa atggtcatta agcccagggc tgaagcgatc
      721 acaaattccg atagtggccc gaattcaatt tttagccagg ataaaaatct tttgcccagg
      781 acaaatgata aacagccgaa gattaaaata accagattaa agaaaaggaa attttttatc
      841 agagcggttt gtctggcaaa aaatgacggc cgctgaaaat tattatgaat ttccgggtaa
      901 ttggcaaaac ggtttttaat gttataggtt aaatcgcctt tgttaagaat aatcggggct
      961 gatttgtcct ggccgttgat gattagttca ttgccgcttc tggtaaccgt cggcccggtg
     1021 taagttccga tgtctttttt tagatataaa atattaccga gtattaaaaa gattatcagg
     1081 ccggccacga ttacataata aattttgaaa ttatccagat tgaatttttt ctttctaaat
     1141 tttaaataaa aaaaccagcc taaagccggc aaaagagtca aagctaaaac aagtaaggct
     1201 gttggataaa gctggccgat ggagtggagg aaattataat gagaccgggc ggttagatac
     1261 ttgaaaataa taaaagaact ccacgagatg ccgaagagag ttagaaatat atataataat
     1321 tttttaaatt tgggtgaaag catttattga gtttaggttg tagtttttag ttatggcagc
     1381 cggtatggtt tttaggttta gttttaggtt tttgaatttt ttaacccttc tgattccccc
     1441 ttactccttc ttttcccccg tgcttccttt tgattcctgt tgtttccttt tccttccgtg
     1501 aaacgcctta tggacgtctc tcaactgctg attagtaata tgggtataga tttgcgttgt
     1561 cgtgatggaa gcgtggccga gcatcgcctg gactgaccgg atgtcagcgc cgttgattaa
     1621 taaatccgtg gcgtaag
//
LOCUS       DASWHZ010000128         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3557630, whole genome shotgun sequence.
ACCESSION   DASWHZ010000128 DASWHZ010000000
VERSION     DASWHZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3557630"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..631
                     /locus_tag="VGA49_02675"
     CDS             77..631
                     /locus_tag="VGA49_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664704.1"
                     /translation="MKIAVFRHGRDASGEKRFLLADEFARLAELRPKLKEKLGGQITD
                     VFSSEATRCSQTALALSGQQSKHPDWLSALAEYPPEVFEKAQAEKIDPTLAYFQMMGV
                     DHGLSQLNNGPAGQHVPQVNLLATTLPASAIVLLVGHGGGLDAVAAHHDLDWTPFKLL
                     GRGEGFILTVGDQGGISFGQYLRW"
     gene            complement(834..3233)
                     /locus_tag="VGA49_02680"
     CDS             complement(834..3233)
                     /locus_tag="VGA49_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX9664705.1"
                     /translation="MFKKLKQNKHFNFILKKLCQGFGKGFFIFGIIAGLTVSNLGISS
                     VEAKTSHAGQKIVQTYNEINIAPNKEIIFEVHFKNIGTATWSNSGPNFVALATISPEK
                     RDSSFADSSWEEVYRPARLTESQVKPGEIGRFKFILKIPAKEGRYVEQFQAVAKNLAW
                     IESAVLEIPINVTSDVPRPRPTTTVTQTSSVASGIVQISKDDYPVRDSGYSGQLVVNS
                     NQNFESEKNGVLSFQLGVKNTGTQVWSNSGSRFVSLYTVRPNYHASRLADASWSSSHQ
                     IKMITDAVKPGEIGYFNFNLKAPGQAGEYIESLRLAVEDYSWFTDGEFKLTVNVVEEK
                     TPVPPALTAGPAPVVVEATDPNYEDGVPYKDLAYQALKLIQSTKELKLRPGQEVQFMV
                     GFKNTGEKTWVNSGSRFVSLYTSRPNYRFSDFAIASTWNSKYQIKMDTASVKPGELGY
                     FTIHLKAPTKPGKYTETFRLAAEEWSWVKGGDLEIPITVLGEDTQNPPVAETPDQNFR
                     PVMEEPIIRVGLYRPEGPVIITANGSYEIRDSNNNLIQSLPPNQISTVKYDSARQVYY
                     LTTAGLDQSYGSYLRFIGADPAVIFEITNYSHPPGWNSSLNDNQFRGVLEIRHNSAKN
                     RVWVINELPMETYLKGIAETTSYSPMEYLKTMTIAARSYAMYHFLKGTKHGNEYFHVD
                     AAYDQVYRGYGLEKRHPKLIQSVDETKGVVVTYNNELAITPYFSQSDGRTRDWSEVWG
                     GKVPWAKSVPDPHNAGRKLLGHGVGISARGALLMDLEDGKTYEEILKYYYTGVELERY
                     Y"
     gene            3322..4746
                     /locus_tag="VGA49_02685"
     CDS             3322..4746
                     /locus_tag="VGA49_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX9664706.1"
                     /translation="MSLTRQIAQNTIIQYGGKVVGTVLALVTIGLMTRYLGQEGFGQY
                     TIIMAFLQMFGILVDLGLTLTTVQMISEPGVDIDKRLSNIFTLRFFSAIIFLGLAPLT
                     VLLFPYPTIVKVGVAITTLSFFFNALNQIMIGVFQKNLKMDRVAIAEIVNRIVLLAGV
                     WLVVYLNYGLLAIMGAIILSNAVQFVLHYIFALKFVKISFRFDWPVWKKIWQTTWPIA
                     ISIVFNLIYLKADTIILSVYQSQAVVGLYGASYKVIDVLIVLPIIFTGLVLPFLTKTW
                     AEKNLDNFKSICQKAFDGVSLLAIPLVVGTLLTARPVMTLIAGRDFVLSGLILQILIF
                     AAFIIYIGALFGHGVIAIGRQKTVIPAYALSAGLSLIGYFIFIPKYSYIGAAGVTIFS
                     EAFVTIYIMIVFYQATKFFPSLGIWLKAILASILMGLVLYFLAGLNIILLIALAVIVY
                     FAALYLFRGISKETVMEIIKPGSQ"
     gene            4901..>5335
                     /locus_tag="VGA49_02690"
     CDS             4901..>5335
                     /locus_tag="VGA49_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664707.1"
                     /translation="MPSEKTNLNILLFSFPFSKTSQKKKPYLTTLVQKLTEKGYNFYL
                     FSSDRNLVSSFRENGLNAYRSLAVVLLLKPILIYHRLFRQTKILICASLAEKIIATPL
                     AKLFGYKIFWLENEFINRRKFHGWWSGWATIIASSQAIKEQLL"
BASE COUNT         1367 a         1250 c         1150 g         1568 t
ORIGIN      
        1 gtaattgaca aattagtaaa tttacattac tatttattgt tgtttccgtt cattcaacac
       61 ccacaaaggg ggcctcgtga aaatagcagt tttcaggcac ggccgtgacg ccagcggaga
      121 aaaaagattt ttgctggctg atgagtttgc ccggctggct gaactccggc ccaagctcaa
      181 agaaaagctg ggtggtcaaa tcaccgatgt tttttcatcg gaagccaccc gatgctctca
      241 aacagccctc gccctgtccg gccaacagtc aaaacatccg gactggctta gcgccctggc
      301 ggagtatccg ccagaggttt ttgaaaaagc ccaagcggaa aaaattgatc cgaccctggc
      361 ctactttcaa atgatggggg tagaccacgg attgagtcag ctcaacaacg ggccggccgg
      421 tcagcatgtt ccccaggtca acttgctggc caccaccttg ccggcaagcg cgatcgtttt
      481 gctggtaggc catggcggcg gactggacgc tgttgccgct catcatgacc tggactggac
      541 gccgtttaaa cttctcggcc ggggagaggg tttcatcctg accgtgggag atcagggagg
      601 gatttctttc ggccagtacc tgcgctggta aacgcctggc attcggccgc cttccactca
      661 aaaaccgcga ctatataata gtagcggttt ctttttttaa attaatttat tattaaatta
      721 cgaattgcga ctaagcagtt caaagttatc aagtccaaag ttcaaagctt ttctacttag
      781 aactttgaac tttggactaa ttcgtaatga gtaatccgta attgactaat ttcttaataa
      841 tacctttcca gctcaacccc ggtataataa tatttcaaaa tttcctcata ggtcttgccg
      901 tcttccaggt ccatcagcaa agcgccgcgg gccgaaatcc ccacgccatg acccaataac
      961 ttccggccgg cgttatgcgg atcagggact gacttggccc aggggacttt gccgccccag
     1021 acttccgacc agtcgcgggt ccggccgtcg ctttgggaga aatacggcgt gatggccagc
     1081 tcattattgt aagtgacgac cacgcctttg gtttcatcca ccgactgaat taattttgga
     1141 tgtctttttt ccaggccata gccccgataa acctgatcgt aagcggcgtc cacatgaaaa
     1201 tattcattgc catgcttggt ccctttcaaa aaatgataca tcgcgtaact ccgggcggcg
     1261 atcgtcattg tttttaaata ttccatcggc gaataactgg tcgtttcagc gattcctttc
     1321 aaataagttt ccattggcag ttcattgatc acccagaccc gattcttagc gctgttatgc
     1381 ctgatttcca aaactcccct aaattggttg tcattcaaag aagaattcca gccgggcgga
     1441 tgactgtaat tggtaatttc aaaaataacg gctgggtcag cgccgataaa tcttaaataa
     1501 ctaccgtaac tctgatccag tccggctgtt gtcagataat aaacctggcg cgctgaatcg
     1561 tatttaaccg tgctaatctg attaggcggc aatgattgaa ttaaattatt attgctgtct
     1621 ctaatttcat aagaaccatt ggcggtaatg ataaccgggc cttccggccg gtatagaccg
     1681 actctaataa tcggctcttc catcaccggc ctgaaatttt ggtcaggcgt ttcagccacc
     1741 ggcggatttt gggtgtcttc gcccagaacc gtaattggaa tttccagatc accgccttta
     1801 acccagctcc actcttcggc cgctaatctg aaagtctccg tgtacttgcc tggcttagtc
     1861 ggcgctttga gatgaatggt aaagtaaccc agttcgcccg gcttaacgct ggccgtgtcc
     1921 attttaatct ggtatttgga attccaggtg gaggcaatgg caaaatcgct gaaacggtaa
     1981 ttgggcctgg aggtgtacaa agaaacaaat ctggagccgc tgttaaccca ggttttttct
     2041 ccggtatttt taaagcccac cataaactgg acttcctgcc cgggtctcaa tttcagctct
     2101 ttggttgact ggatcagctt cagagcctga taagccaagt ccttatacgg cacgccgtct
     2161 tcataattag ggtcggttgc ttcaactacg accggcgccg gccccgccgt caatgccggc
     2221 gggacgggcg ttttttcttc aacaacattt actgtcagct tgaattcgcc gtcagtaaac
     2281 cagctgtaat cttcgacggc cagccttaaa ctttcaatat actccccggc ctggccgggc
     2341 gcttttaaat taaagttaaa ataaccgatt tcgcccggct tgaccgcgtc agtaatcatt
     2401 ttgatctggt ggctggaaga ccaggaagcg tcggccagcc tgctggcgtg gtaattgggt
     2461 cggacagtgt ataaagaaac aaatctggag ccgctgtttg accagacctg ggtgccggtg
     2521 tttttaacgc caagctgaaa actcaaaacc ccattttttt ctgactcaaa attttgattg
     2581 gagttaacaa ctaactgacc agaatagccg ctgtctctga ctggataatc atccttagaa
     2641 atttggacga tcccactggc gactgaactg gtttgagtaa cagttgttgt cggccggggc
     2701 ctgggaacat ctgaagtaac attaatcgga atttccaaaa ccgcgctttc aatccaggcc
     2761 aggtttttgg cgactgcctg aaattgttca acataccggc cttctttagc cggaattttt
     2821 aaaataaatt taaaccgccc gatttcgccg ggtttgactt gtgattcagt caaccgggcc
     2881 ggccggtaaa cttcttccca ggaactatca gcgaaagaac tgtctctttt ttccggactg
     2941 atcgtcgcca gggccacaaa attgggaccg ctgttgctcc aggtggctgt gccaatattt
     3001 ttaaaatgga cttcaaaaat gatttctttg ttgggcgcga tattaatttc attataagtt
     3061 tgaacgattt tctggccggc atgggaggtt ttagcctcaa ccgagcttat ccccaaatta
     3121 ctcacagtta atcccgcgat gatcccaaaa ataaaaaatc ctttgccaaa gccctggcaa
     3181 agtttcttta aaatgaaatt aaaatgttta ttttgtttta attttttaaa catacaatat
     3241 cacgctccaa taacttctca atcctacaat cattatagca aaaaatggag taaaaatcaa
     3301 tcaggtgata taattttatt aatgtctcta acacgacaaa tcgctcaaaa cacgatcatc
     3361 caatacggcg gcaaggttgt cgggactgtt ttggctttgg tgactattgg cttaatgacc
     3421 cggtatctgg gccaggaagg ttttggtcag tacacgatca ttatggcttt tctccagatg
     3481 ttcggcattc tggtggatct ggggctgacc ctgaccacgg ttcagatgat ttccgagcca
     3541 ggcgttgata ttgataaaag attaagcaac atttttactt taagattttt ttcagcgatt
     3601 atctttctgg ggctggcgcc cttaacagtt ttgctctttc cctatccgac aatcgttaaa
     3661 gtcggcgtgg ccattaccac gctatcgttc tttttcaacg cccttaatca aattatgatt
     3721 ggggtctttc aaaagaattt aaaaatggac cgggtggcca tcgccgaaat tgttaatagg
     3781 atagttcttt tggccggcgt ctggctggtg gtttatttaa actatgggtt gctggcgatc
     3841 atgggcgcca ttattttaag caacgccgtc cagtttgttc tccactatat cttcgctctt
     3901 aaattcgtca aaatttcttt tcggtttgac tggcctgtct ggaaaaaaat ctggcaaacc
     3961 acctggccaa tcgccatatc cattgttttc aatttaattt acctcaaagc ggacacgatc
     4021 attctgtcag tctatcaaag ccaggcagtt gtcggcctct atggagccag ttataaagta
     4081 attgatgttt taattgtcct gccgattatt tttaccgggc tggttctgcc gtttttgacc
     4141 aaaacctggg ctgaaaaaaa tctggataat tttaaatcaa tttgccagaa agcttttgac
     4201 ggcgtcagtc tgctggccat ccctctggtc gttggaacgc tcctaaccgc ccgcccggtg
     4261 atgactttga ttgccggcag ggactttgtt ttgtccggtc tgattttgca aattttaatt
     4321 tttgccgctt ttataattta tatcggggct ctttttggcc acggggtcat cgccattggc
     4381 cggcagaaaa ccgtcattcc ggcctacgct ttatcagccg gcctgtcgct aatcggctat
     4441 tttattttta ttccaaaata ttcctacatt ggcgcggccg gcgtcacaat tttttccgaa
     4501 gcttttgtga caatttatat aatgattgtt ttttaccagg ccacaaaatt tttcccttcc
     4561 ctgggaatct ggcttaaagc aattttagct tcaattttaa tgggtctggt cctttacttt
     4621 ttggccggct tgaatataat actgctaata gccctggcgg tgatagtcta tttcgcggct
     4681 ctgtatttat tcaggggcat ttccaaagag acggtgatgg agataataaa accaggcagt
     4741 caataaatcc aaatttcaaa gcccaaatgt caaatcaaat ccaaagccca aatgtcaaaa
     4801 ttctggattt tggattttag atttcatttg aaacaatttg gattttgaaa tttgaaatta
     4861 aaaagcatta ataaaatagc tgcctagaaa taaaacttaa atgccctcag aaaaaacaaa
     4921 cctgaatatc ttgcttttct ctttcccctt ttcaaaaacc agccaaaaaa agaaaccata
     4981 tctgaccact ttggttcaaa agctgactga aaaagggtat aatttttatt tgttttcttc
     5041 tgaccgaaat ttagtttcct ctttcaggga aaatgggtta aacgcttacc ggtcattagc
     5101 cgtcgttctg ctgttaaagc caatccttat ctatcaccgg ctattccggc aaactaaaat
     5161 attaatttgt gcttctttag ccgaaaaaat catcgccacg ccgctggcca aactgttcgg
     5221 ctataaaatt ttctggctag aaaatgagtt tatcaaccgg cgtaaatttc acggctggtg
     5281 gtcaggctgg gcaacgatta ttgccagcag tcaggcgatt aaagaacagc tcctg
//
LOCUS       DASWHZ010000129         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_622167, whole genome shotgun sequence.
ACCESSION   DASWHZ010000129 DASWHZ010000000
VERSION     DASWHZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622167"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VGA49_02695"
     CDS             <1..1403
                     /locus_tag="VGA49_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664708.1"
                     /translation="SGALIILLQFNHLTIGDSQLLNIIKPYLLIGLLLALLISLVILI
                     SRHKSVLLKELPRILLVMALLILPWLPWTIKTAFELNQPSLNFILTHGLNVEYPWPGV
                     DLSQCSQDNLNNPELERYFGVQKGFNKILALPWVLTMTQQQNVQPVFTEISYVWLAVI
                     VGFGWLLLVKKPDQPEKYLLIYTGAALIIWLLTSYGIPWYGLNLFVGLLGLIALLIKR
                     SGQPGRLSKIVQAIILLIIISWILLFLPHRLSFATPSYVIYHQLNLAGPAELIDQNIK
                     EAEIIKIINNDQTAKIYNVETFISYFVKNNDQRVYGDAHLAGWQCAWNSYAGKPDFSS
                     DDYFRARSALEKLNFDKNKCSQQGCPELASIETAIKLKEKEISQLIFDIEPFFDKIIT
                     DLQNQGFKYFILGPGANVERNFNGPIHQIFKMALIVLEEKADLIYSLSGQPPGFDPQN
                     FPRTFNDIYLYELKAK"
     gene            complement(1389..>1794)
                     /locus_tag="VGA49_02700"
     CDS             complement(1389..>1794)
                     /locus_tag="VGA49_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664709.1"
                     /translation="LFYSSNLIIDNALVLADRWNVRQSLIGILLIGFGTSLPELSVAF
                     SAASQKALKLTVGNLIGSNIIDLLLITGLTGTLSGINISRQLLFFDVPALFVISLTAI
                     ILFSTQKRLIKKEGLALILLFGVYVGFKLFGF"
BASE COUNT          529 a          386 c          361 g          518 t
ORIGIN      
        1 tttcaggagc tttaattatt ctgttgcaat ttaatcatct gacaattggt gacagccagc
       61 ttttgaatat tatcaaacca taccttttaa tcgggctgct cctggcgctt ttgatcagtt
      121 tagtaatttt aatttcaaga cataaatcag tccttttgaa agaactaccc agaattcttt
      181 tggtgatggc cctgctgatc ctgccctggc tgccctggac gattaaaacc gcttttgaac
      241 ttaaccagcc cagtctaaat tttattttga cgcatggttt aaatgtagaa tatccctggc
      301 cgggagttga tttaagccag tgtagccagg ataatcttaa caatcctgaa ttggaaagat
      361 attttggcgt tcaaaaaggc tttaataaaa ttctggcttt gccctgggtc ctgacaatga
      421 cccagcagca aaatgtccag ccggttttca cggaaatctc ctatgtctgg ctggcggtga
      481 tcgtgggctt tggctggctg ttacttgtca aaaaaccgga tcagccggaa aaatatttgt
      541 tgatttatac cggcgcggcc ttgattatct ggctgctgac atcttatggc atcccctggt
      601 acgggcttaa tctttttgtt ggactgctgg gactgatcgc cctgctgatt aagcggtcag
      661 gccagcccgg ccgcttatca aagattgtcc aggcgattat tctgttgatt atcatcagct
      721 ggattttatt atttctgccc catcgtttat ctttcgcgac tccttcctat gtcatctatc
      781 atcagctgaa tttggccggg ccggcggagc tgattgatca aaatattaaa gaagcagaaa
      841 ttattaaaat tattaataat gatcaaaccg ccaaaattta taacgtggaa acttttatca
      901 gctatttcgt aaaaaataac gatcagcggg tttacggcga cgcccatctg gccggctggc
      961 aatgcgcctg gaacagctac gccggaaagc ctgacttcag ttcggacgac tattttcggg
     1021 ccaggtctgc tttggaaaaa ctgaattttg acaaaaataa atgcagccaa caaggctgcc
     1081 cggaactggc ctcaattgaa accgccataa aacttaaaga aaaagaaatc agtcagctga
     1141 tttttgatat tgaaccattt tttgacaaaa ttatcactga tcttcaaaac cagggcttta
     1201 aatattttat tctgggaccg ggagccaatg ttgaaagaaa ttttaacggc ccaattcatc
     1261 agatatttaa aatggccctg attgttcttg aagaaaaagc cgatctaatt tattctttga
     1321 gcggccagcc gcccggtttt gaccctcaaa attttcccag aaccttcaat gatatttatt
     1381 tgtacgaatt aaaagccaaa taatttaaaa ccaacgtaaa ctccaaaaag aaggattaaa
     1441 gccagtcctt cttttttgat taatcttttt tgagtgctga ataaaattat cgctgtcaaa
     1501 gagataacaa acaaagccgg aacatcaaaa aacaacagct gtcgactgat attgatgcct
     1561 gataatgttc cggtcagtcc ggtgatcaac aggagatcaa taatattact gccgattaaa
     1621 ttgccgactg tcaacttcaa cgctttttga gaagcggcgc tgaaggcgac cgacagttcc
     1681 ggcaggctgg tcccaaaacc aatcagtaaa atgccaatca aagactgccg cacattccac
     1741 ctgtcggcca aaaccaaagc attatcaata atcagattgg aagagtagaa caaa
//
LOCUS       DASWHZ010000130         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2123316, whole genome shotgun sequence.
ACCESSION   DASWHZ010000130 DASWHZ010000000
VERSION     DASWHZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123316"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..853
                     /locus_tag="VGA49_02705"
     CDS             137..853
                     /locus_tag="VGA49_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9664710.1"
                     /translation="MNHIFAIIKKEIRVYFNSPAAYIFITVFLVLSSWLYFRSLFLVG
                     QSSMRGYFDLLPWIFLFLIPAVSMRLWAEEKKLGTDEILLTWPVKDWEVVVGKFLASF
                     IFLLITLAGSLIIPVILFILGTPDLGVIIGGYLGAIFMMAAYLAIGLYASSLTNNQIV
                     AFIAAVAVSFVLYIIGREFVLIFAPSFLTGLLRYAGLSSHFDSIVRGVIDSRDIVYYL
                     SVVAVFLLLNLRSIQSRYWK"
     gene            1082..>2194
                     /locus_tag="VGA49_02710"
     CDS             1082..>2194
                     /locus_tag="VGA49_02710"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX9664711.1"
                     /translation="MKLDPKSKYSIKSSFVIILIIAAIVAANFIFSKYFVRFDLTEDK
                     QYVISDVSKELVKNLQEPVAIKVYFSTKLPPQLLAVTQQVKDILDEYRLNSGGKVSID
                     YLDPADNPETEQEAISKGVQEVQFNFFEKDKVQVQKGYLGIVFSSGDKSETIPVVQDV
                     ASIEYDITSALKKLLADEIKTVGFLTGHQEHGFATFFVEQQEGKRDDYVTLNQVLRQT
                     YQVTTVDISAGQPITDVDTLIVAGPQEALSQRELFEIDQFIMNGGKVVFLVDRVNILG
                     SLLAIPLSTGLEDLLANYGVTVKDNLVLDQINEVAAFTAGGLQYMLEYPYFVKLIRDN
                     FDLENPIVAKLESIVLTWASSLEPVEQEGIETTVLVK"
BASE COUNT          561 a          408 c          518 g          707 t
ORIGIN      
        1 aatccaaatg tcaattcaaa tcaaaagctc aaatgttaaa ataaaaaaat ttggatttta
       61 gcccgccccg ctctgcggag cagggccggg gattttggat ttaaattgaa atttgagctt
      121 tgatatttgt cattatatga atcacatttt tgccattatc aaaaaagaaa tccgggttta
      181 ttttaactcg cccgcggctt atatttttat taccgtattt ttagtattaa gctcctggct
      241 gtattttagg agtctttttt tggtcggcca gtcgtcaatg cggggttatt ttgatttatt
      301 gccctggatt tttttgtttt taattccggc ggtcagcatg cggctctggg ccgaggaaaa
      361 aaaactgggc accgatgaga ttttactgac ctggccggtt aaagactggg aagtggtggt
      421 gggtaaattt ttggccagtt ttatattttt actgatcact ttggccggtt cattaattat
      481 tcccgttatt ttatttattc tgggaacgcc tgatttggga gtgattattg gcggatatct
      541 gggcgcgatt tttatgatgg cggcgtatct ggcgattggt ttgtacgctt ccagcctgac
      601 taataatcag attgtggctt ttatcgcggc cgtggccgtt agttttgttt tgtatataat
      661 tggccgggag tttgttttaa ttttcgcccc gtcgttttta accggccttt taagatacgc
      721 tggtttgtcc agccattttg acagcatcgt ccggggagtg attgattcgc gggatattgt
      781 ctattacttg tcagtcgtgg ccgtgttttt attgttgaat ttacgcagta ttcaatccag
      841 gtattggaaa taaaacaggc aacaggcaca aggcacaagg caataggcta ggcaaaaggg
      901 ggcaggcaaa aggatagaat tgaaaatttt ttgattgatt actaagtaca tcttgcctat
      961 tcttatttgc ctgatgccca tttaataaat aattccaacc ctattgcctt gtgcctcgtg
     1021 ccttgtgcct atatcttgtg cctcgtgcct tgtgcctcgt gccttgtgcc ttgtgaatat
     1081 tatgaaactt gacccaaaat ccaaatactc aatcaaatca tcattcgtga taatcttgat
     1141 tatcgcggct attgtcgctg ctaatttcat attttccaaa tattttgtca ggtttgacct
     1201 gactgaagac aaacaatacg taatttccga tgtttccaaa gagttggtta aaaatttaca
     1261 ggaaccagtc gcgatcaaag tttatttttc aaccaaactg ccgccccagc tcctggccgt
     1321 cacccagcag gtcaaagata ttttggacga gtaccggctt aattcggggg gtaaagtttc
     1381 aattgactat ctggatccgg ccgataatcc cgagaccgaa caagaagcaa tctctaaagg
     1441 cgttcaggaa gttcagttta atttttttga aaaagacaag gtccaggttc aaaaaggcta
     1501 tttgggaatt gttttttcgt ctggggataa gtctgaaaca attccggtcg ttcaggacgt
     1561 ggccagtatt gaatacgaca taacttcggc tttaaagaaa ttattggcgg atgaaattaa
     1621 aacagtcggt tttctgaccg gacatcagga acacggtttt gccacctttt tcgtggaaca
     1681 gcaggaggga aaaagggatg attatgttac tttaaaccag gttttaaggc agacttacca
     1741 ggttacgaca gttgacatca gcgccggcca gccgataact gatgttgata cgctgattgt
     1801 cgccgggccc caggaagcat tgagccagcg ggagctgttt gaaattgatc agtttatcat
     1861 gaatggcggg aaagttgttt ttctggttga tcgggttaat attttgggga gtttattggc
     1921 aattccttta tccacaggtt tggaggatct gctggccaat tatggggtaa ctgttaaaga
     1981 taatctggtt ttggatcaaa ttaacgaagt agccgctttt accgccggtg ggcttcaata
     2041 tatgctggaa tatccttatt tcgtcaagct gatcagggat aattttgacc tggaaaaccc
     2101 aattgtcgcc aaactggaga gcattgtctt gacctgggcc agttcgctgg agccggttga
     2161 acaagagggg attgaaacga cggttttagt taaa
//
LOCUS       DASWHZ010000131         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_625208, whole genome shotgun sequence.
ACCESSION   DASWHZ010000131 DASWHZ010000000
VERSION     DASWHZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625208"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VGA49_02715"
     CDS             complement(<1..955)
                     /locus_tag="VGA49_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX9664712.1"
                     /translation="MKQSRLFGKTSKTVSKDEVSANAQLLFKAGFVNKLMAGVYSYLP
                     LGWRVHQRVASIVREEMNKISAQEVFLPALHPRENWEQTKRWDSFDVLFKIESQTGNS
                     YVLGPTHEEVIVPLVKKYVQSYKDLPVCLYQIQTKFRDEKRAKSGLLRGREFSMKDLY
                     SFHATQEDLETYYQRVVESYKQIFSRCGLEALLTEASGGAFTEKFSHEFQVLTPYGED
                     TIIFCPSCHWAQNKEIARQKAGDKCSRCQGALESSRAIEAGNIFDLGTKFSKDFNLTY
                     ADEQGREHLVIIGCYGLGISRLLGAIAEVFHDDRGLIWPQNI"
     gene            complement(1071..2183)
                     /gene="rseP"
                     /locus_tag="VGA49_02720"
     CDS             complement(1071..2183)
                     /gene="rseP"
                     /locus_tag="VGA49_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX9664713.1"
                     /translation="MITTIIVFILVLGLLVFVHELGHFVAAKKMGVRVDEFGLGFPPK
                     LIGIRKGETLYSLNLLPLGGFVRIKGETGEDKDDPDSFSHKKIWQRSIILFSGVAMNF
                     VTAIILVAIGFMIGLPLPLDDAESFAKAKISDPRIQVVSILKDSPAEQADLAVGDVIL
                     KINDQSFAELEQLQGFLEEREGAVSVVVKRGQEELARTIETKRLAETGKFGLGVGLVK
                     SGIVSFPWHLALWHGLERSVFITKEVVIAFYTLFKNLIVGQQIAVDVSGPVGIAIITG
                     QVTKLGFVYILQFMALLSINLAVINILPFPALDGSRLFFLAVEKIRRKQLDRRVESLI
                     HTFGFSVLILLMVFITYRDVVKYGGDIWSLVSGFFS"
     gene            complement(2209..2763)
                     /gene="frr"
                     /locus_tag="VGA49_02725"
     CDS             complement(2209..2763)
                     /gene="frr"
                     /locus_tag="VGA49_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9664714.1"
                     /translation="MNDIIKKHQAGYQDLISHLREDLKSLRTGRANPAMIENIVVEAY
                     GVKTSLNQLASISLADAKTLVVQPWDKNILKDIEKAIVQANIGINPVNEGSLLRLSLP
                     QLTEENRKELINILHQKGEKTKIAIKVLRDKVKEEIVKAEKNKEITEDDRFDYLKELD
                     EFTRDYNDQVKAVMDDKEKEIMTI"
     gene            complement(2769..3734)
                     /locus_tag="VGA49_02730"
     CDS             complement(2769..3734)
                     /locus_tag="VGA49_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HEX9664715.1"
                     /translation="MQFEWPVIGHQNLTNHLQQSILTNKLSQAYIFYGPDKVGKKTVA
                     RHFLYSVLCQDQSVRPCQSCLHCRQLEKNIHPDVFKIGPEPDKKKISIDQIRRLQTSL
                     GLGSLLNSYKIALIDQSDRLTTEAGNSLLKTLEEPSPKTIIILITANLEALPKTIASR
                     CQLYQLRPVSSQKIHDYLLASGARRRQAEEITSLARGRAGLAVSYFQNQDEFKVVQKT
                     VKKLTQIFRSEFFKRFDLIEGVADQPNQLIFQLRDLIRDLILIKAGADDYLTYSFIKK
                     DLINLASDISLNRLLKINKKINQARPYLNLNVNARLLLENLIISI"
BASE COUNT         1108 a          893 c          735 g         1090 t
ORIGIN      
        1 cgatattttg gggccagatt aatccccggt catcatgaaa aacttcagca atcgctccca
       61 gtaaccggct gatgcccagc ccgtaacagc caataatcac caaatgttcc cgcccctgct
      121 cgtcagcata tgtcagatta aagtcttttg aaaatttagt tcccaaatca aaaatattac
      181 cggcctctat cgctctggat gactccagag cgccctggca acgcgaacat ttatcccccg
      241 ccttttgacg ggcaatttcc ttattctggg cccagtgaca gctgggacaa aagataatgg
      301 tatcttcccc ataaggcgtc aacacctgaa attcatggga aaatttttcc gtaaaagcgc
      361 cgccggaagc ttcggtcaat aaagcctcca aaccgcagcg gctgaaaatt tgcttatacg
      421 attcaactac tctctgataa taagtttcaa gatcttcctg ggtagcgtgg aaactgtaaa
      481 ggtctttcat ggaaaattcc cggcctcgca gcaaaccgga tttggctctt ttttcatccc
      541 taaatttagt ctgaatctga taaaggcaga ctggtaagtc tttgtaagac tgaacatatt
      601 tttttaccaa cggaacaatc acctcttcat gggtcgggcc caggacgtaa gaattgccgg
      661 tctgtgattc tattttaaat aaaacatcaa aactatccca gcgtttggtc tgttcccagt
      721 tttccctggg atggagggcg ggtaaaaaaa cctcctgagc actaatcttg ttcatttctt
      781 cccggacaat gctggcgact ctctgatgaa cccgccagcc gagcggtaaa taactataaa
      841 cgccggccat cagtttattg acaaaaccag ctttaaataa aagctgggcg ttggcgctga
      901 cctcatcttt ggaaacagtt ttggatgttt ttccaaataa tcttgattgt ttcatagctg
      961 ttgataacgt taatatttta aaagtttatg gctcttttgt caaaaagtca ccccgacgcg
     1021 cgtcggggcg agtgacgggg cataggccca aaaaaaatca acaagtcatt ttaactgaaa
     1081 aaaccactga ctaaactcca gatatcgccg ccatatttaa ccacatcccg gtaagtgata
     1141 aaaaccatta aaaggattaa aactgaaaaa ccaaaagtat gaattaaact ttcaacccgg
     1201 cggtccagtt gcttccgacg gatcttttca accgccaaaa aaaacaaccg gctgccgtcc
     1261 aaagccggga aaggcaaaat attaatcacc gccaggttaa ttgacagtag cgccataaac
     1321 tggaggatat agacaaaccc cagcttggtt acctggccgg tgataatggc aatccccacc
     1381 ggaccggaaa catcaacggc aatctgctgg ccaacaatta aatttttaaa tagggtgtaa
     1441 aaagcaataa cgacttcttt ggtaataaaa accgaccgtt ccagcccgtg ccaaagagct
     1501 aaatgccagg gaaaggaaac tatgccgctt ttaaccaggc caacgcctaa accaaacttg
     1561 ccggtttccg ccagtctttt ggtttcaatc gttctggcca gctcctcttg tcctcttttg
     1621 acaacaactg ataccgcgcc ttccctttct tccagaaaac cctgaagttg ttccagctcc
     1681 gcgaaagact ggtcattaat ttttaaaata acatcgccaa cagccaggtc cgcctgctcg
     1741 gccggcgaat cttttaaaat tgacaccacc tggatccggg gatcactgat tttggctttg
     1801 gcaaaacttt cagcgtcatc aagcggcagg ggcagtccaa tcataaaacc aatggccacc
     1861 aaaattatag cggtaacaaa attcatggcc acgccgctaa acaaaattat tgatcgctgc
     1921 caaatttttt tatggctaaa actgtccgga tcatctttgt cttccccggt ttcccctttt
     1981 attctgacaa acccgcccaa aggcaataaa tttaaactat ataaagtttc accttttctg
     2041 atgccaatta atttgggagg aaaccccaaa ccaaattcat cgacccgaac ccccattttt
     2101 ttggccgcca caaaatggcc gagttcatgg acgaacacta ataatcccag gactaatatg
     2161 aatactatta ttgtagttat catttgtaat aaaaatatta gcttaacact aaatcgtcat
     2221 tatttccttt tctttatcat ccatgaccgc cttgacctga tcattgtaat ctctggtgaa
     2281 ttcatccagc tctttcagat aatcaaacct gtcatcttca gtaatttctt tatttttttc
     2341 cgctttgaca atttcttctt taaccttatc gcgtaaaacc ttgatggcaa tcttggtttt
     2401 ctcccctttt tgatgaagaa tattgatcag ctccttacgg ttttcttcag tcagctgtgg
     2461 cagagacaat cgcagcaatg aaccctcatt aaccggatta atcccgatat tagcctggac
     2521 aatcgccttt tcgatatctt tcagaatatt cttatcccag ggctgaacaa ccaaagtttt
     2581 ggcgtcagcc aggctgatgc tggccagctg atttaaagag gttttaacgc cgtaagcttc
     2641 aacaacaata ttttcaatca tcgccggatt agcccggccg gttcgcagtg atttcaagtc
     2701 ttctctaaga tggctaatca gatcctgata accagcctga tgttttttta taatatcatt
     2761 cataacattt atatgctaat tattaaattt tccagcaata gcctggcatt aacgtttaag
     2821 ttaagataag gccgggcctg attaattttt ttattaattt ttagcaggcg atttaggctg
     2881 atatctgacg ccagattaat taaatctttt ttaataaaag aataagttaa ataatcatca
     2941 gcgccagctt taattaaaat caggtcccgg atcaaatctc ttagctgaaa gataagctga
     3001 ttgggctgat cagccacgcc ctcaatcaga tcaaaccttt taaaaaattc tgatctaaaa
     3061 atttgagtca attttttaac ggttttttgg acaactttaa attcgtcctg attttgaaaa
     3121 tagctaactg ccagtccggc ccggccccgg gccagactgg tgatctcttc agcctgccgg
     3181 cgcctggcgc cgctggccag gagataatca tggatttttt gactggaaac gggccgcaac
     3241 tgataaagct gacaacggga agcaatggtc ttaggcaggg cttccaggtt agcggtaatt
     3301 aggattataa tcgttttggg gctgggctct tccaaagtct ttaataaaga attgccagcc
     3361 tcggttgtca gccggtccga ctgatcaatc agggcgattt tgtatgaatt taacaaagag
     3421 cccaggccta aactagtctg gagccgcctg atttgatcaa tgctaatttt tttcttgtca
     3481 ggttccggcc ctattttaaa aacatcagga tgaatgtttt tttccagctg gcggcaatga
     3541 aggcaggact gacaaggacg aactgactga tcctgacaca gaactgaata taaaaaatga
     3601 cgggcgactg tttttttacc aaccttatcc ggaccgtaaa aaatataagc ctgtgacagc
     3661 ttgtttgtta aaatgctttg ctgcaaatgg ttggttaaat tttgatggcc aataaccggc
     3721 cactcaaatt gcatatagtt taatatagca aatttaaatt acttagtcga taaaattgac
     3781 cgtttgtaag attccaaatt ttccagggca atgccggtgc ccttgg
//
LOCUS       DASWHZ010000132         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2126817, whole genome shotgun sequence.
ACCESSION   DASWHZ010000132 DASWHZ010000000
VERSION     DASWHZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126817"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGA49_02735"
     CDS             <1..444
                     /locus_tag="VGA49_02735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9664716.1"
                     /translation="KIAGDGPQLQEIKAFIERYDLKNVELLGYLPTDELQQVIRQSRL
                     VIVPSVWHENYSMTLLEAAALGKPLIASNIGGNSEIVKDQFTGLLFEPGNYSQLAEKI
                     KFLYDNESLIREMGQNARKQVEKENSSEQHYSKLMEIYKRAMDKI"
     gene            451..2529
                     /locus_tag="VGA49_02740"
     CDS             451..2529
                     /locus_tag="VGA49_02740"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX9664717.1"
                     /translation="MLLNQSIKFKIVIFLTGLILLLPNFSLAAHQPNHQFPKLSNYFL
                     KWGVSDQEAKELARWDLLIIDMEAQVYTPQNLKKIRQYNPDVKILAYITSQEIRKDAP
                     ALDKRTLRSQLYGGIHQQWYLKNSSGQNLNWWPGTLLLNITDQSPGNYGWNDYLPQFV
                     ADKILSTGLWDGVFYDNTWPDVSWVSGQIDLNNDQAAEPPLVVDAAWRSGMEKILRET
                     RLLAGPHVLIVGNKGDSYFSHLNGSLIENFPGYGWTEQLGNYQRINFQGVRPAFGIIN
                     SNTNNTGHQSDYRRMRFGLCSALLGDGYFSFDFGDQDHAQVWWYDEYSAFLGEPKSTA
                     VNLLENNLTRLKPGLWRREFANGLVLCNSTYDQQVFRLEGEFEKIHGHQDRAHNDGSI
                     TEKIVLNPRDGIVLLRPIERIIGSSFKNGSFARVFNSQGQTIRTGFFAYDSQYRGGRN
                     IIIKDINADGQLETVVADDSKVEIYNSQGIKTSIFYPYTERYNLGINISVGDLNGDGT
                     MEIVTGTERGGGPQIRIFNNQGKLINPGFFAYGKDFRGGVNVAIGDLNGDGTSEIIAG
                     AGVGGGPHIRVFNKDGRLINPGFFAYDKGFRGGVNVAVGDIDGDGVAEIIAGSGAGGG
                     PEVRVFNKDGQLLSAGFFAFEPIKKSGVEVIANDLDGDGKAEIIATTTDVFTFSGLSP
                     QGGSAFGGNF"
     gene            2547..>2858
                     /locus_tag="VGA49_02745"
     CDS             2547..>2858
                     /locus_tag="VGA49_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664718.1"
                     /translation="MTETIDDKKIMSVSGEIIPPGKRARQPRRQWQRGQQINIGTLNI
                     FIHPAKKFIGQPMKKRYDKHYHRQLKHGRKHLAADIILAGLVIGLVIFIGYVLFLYGR
                     GI"
BASE COUNT          775 a          587 c          725 g          771 t
ORIGIN      
        1 aaaattgccg gcgacggacc ccagctccag gagattaaag cttttattga acgttatgat
       61 ttaaaaaatg ttgagctgtt ggggtatctg ccgactgacg agttacaaca ggtaatcagg
      121 cagtctcgtt tggtgatcgt cccgtcagtc tggcatgaaa actattcaat gactctgctg
      181 gaagcggccg ctctgggcaa gccgctgatc gcctctaaca tcggcggcaa ttcagaaatt
      241 gttaaagacc agtttaccgg cctgctgttt gaaccgggca attattccca gttggccgaa
      301 aagattaaat ttttgtatga taatgaaagt ttaatcaggg aaatggggca gaacgcccgg
      361 aaacaagttg agaaagaaaa cagttcagaa cagcattata gtaagctgat ggagatttat
      421 aaaagggcta tggataaaat ttgataatgt atgttactaa atcaatcaat caaattcaaa
      481 atagttatat ttttaaccgg cctgattttg ctgttgccga atttttctct ggccgctcac
      541 cagcccaatc atcagtttcc taaactaagc aactattttt tgaaatgggg cgtctctgat
      601 caggaagcca aagagctggc tcgttgggat ttactgatta ttgacatgga agcccaggtt
      661 tatactcccc agaatttaaa aaagatcagg caatataatc ctgatgttaa aattttagct
      721 tacattacct cccaggaaat cagaaaagac gctccggctc tggataaacg aactttgaga
      781 agccagcttt acggcgggat tcatcaacaa tggtatttaa aaaacagctc cggccaaaac
      841 ttaaactggt ggccgggaac tttacttttg aacattaccg atcagtcgcc cggcaattac
      901 ggctggaatg attatttgcc ccagtttgtg gctgataaaa ttttgagcac cggcttgtgg
      961 gacggagtgt tttatgacaa cacctggccg gacgtcagct gggtcagcgg ccagattgat
     1021 cttaataatg accaggcggc cgagccgccg ctggttgttg acgccgcctg gcggtccggg
     1081 atggaaaaaa ttttacggga aacccggcta ctggctggac cgcatgtttt aattgtcggc
     1141 aataaaggcg acagttattt ctcacattta aacggcagct taattgaaaa ttttcccggc
     1201 tacggctgga cagaacagct tgggaattat cagcgaatta attttcaggg cgtcaggcca
     1261 gcttttggca ttattaattc caacaccaat aataccggtc accagagcga ttaccgccgg
     1321 atgagatttg gtttatgttc ggctttgctg ggcgatggtt attttagttt tgattttggc
     1381 gaccaggatc atgcccaggt ctggtggtat gatgaatatt ccgctttttt gggcgagcca
     1441 aaatcaaccg ctgttaatct tctggaaaat aatttaaccc ggctaaagcc tggtttatgg
     1501 cgccgggaat ttgccaacgg tctggttttg tgcaattcaa cttatgatca gcaagtattc
     1561 cggctggagg gagaatttga aaagattcat ggtcatcagg accgggctca taatgacggt
     1621 tcaattactg aaaaaattgt tttaaatccc agagacggta ttgttttact gcggccgatt
     1681 gaaagaatta tcggcagctc gtttaaaaac ggctcgtttg cccgggtttt taacagccag
     1741 ggccagacta tccggaccgg ttttttcgct tatgacagcc agtatcgggg cggccgaaat
     1801 atcatcatca aagatattaa tgctgacggc cagctggaaa cagtcgtcgc tgatgacagc
     1861 aaagttgaaa tttataacag tcagggaatt aaaacttcaa ttttttatcc ttacactgaa
     1921 cgatataatc tggggattaa tatcagcgtt ggcgatttga acggcgacgg caccatggaa
     1981 attgttaccg gcaccgaacg gggcggcggt ccgcagatca gaatttttaa taatcaggga
     2041 aaattaatca atccgggatt ttttgcttac ggcaaagatt ttcggggcgg cgttaatgtc
     2101 gctattggcg atctgaacgg cgacggcacc agtgaaatta tcgccggcgc cggggtgggc
     2161 ggcggtccgc acattcgggt tttcaataag gacggccggc tgatcaaccc tggtttcttc
     2221 gcctatgaca aaggttttcg gggcggcgtt aacgtggcgg tgggggatat tgacggggac
     2281 ggggtggcgg aaattatcgc cggttcggga gccggcggcg gcccggaagt tcgggttttt
     2341 aataaagacg gtcagttgct gtcagccgga tttttcgctt ttgagccgat caaaaaatca
     2401 ggggtggaag tcattgccaa tgatttggac ggtgacggga aagctgaaat catcgccacc
     2461 acgacggatg tctttacatt ttcaggttta tccccccaag gtggatccgc cttcggcgga
     2521 aacttttaat ttgttataat taagatatga ctgaaaccat cgatgacaaa aaaataatga
     2581 gcgttagtgg ggaaatcatt ccaccgggga aaagagcccg ccagccccgc cgccaatggc
     2641 agcgaggcca gcagataaat attggaacgc ttaatatttt tattcatccg gctaaaaaat
     2701 tcattggtca accgatgaag aaaagatacg ataaacatta tcatcgtcaa ctaaaacacg
     2761 gccggaagca tctggcggct gacattattt tggccggcct ggtgattggc ttagtgattt
     2821 ttattggtta tgttttgttt ctatatggcc ggggcatt
//
LOCUS       DASWHZ010000133         4065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_259434, whole genome shotgun sequence.
ACCESSION   DASWHZ010000133 DASWHZ010000000
VERSION     DASWHZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259434"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="VGA49_02750"
     CDS             <1..2147
                     /locus_tag="VGA49_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9664719.1"
                     /translation="NQPNSETPVRQAAGFSAGDILINEFVADPGDNETEWVELRNMTH
                     SIINLESWTVADGSGKITELAGLIEPENYFVIEKPKGVLNNTGDLIILKFKETVIDRV
                     AYGDWDDGDIADNAPKAADPNSVARTDFNDADHDFNDFQVTTTPTKGGTNIISAPIIS
                     EEKNNSSETLKTPPPVDPTIYKHLIINEFLPNPAGPDATDEFIELKNNGSGPVDIENL
                     IIQDNSSKKHKINQKDFDSTLIQPGGFFVINRSVSGIALNNTGGDAVKLFSPDELLIE
                     QIDYFEKAEEGLSYSRDQDDDWFWTAAITPGAANSLPELITIEEPNPGSADESIATAE
                     PAEKQTDYPPIMITEVLPNPAGSDSGEWLELYNPNDFEIDLTGWRIDDEEGGSRPHKI
                     KDTVIGPRQYLVLPRQQTKLALNNSGDSVRLFDPNNTLIDLTDYEAARENSSWALSDD
                     YEWSWTANLTPQDANIFTQPATAADKKNKNKQIIQTTLKQARENDPGDLIQVEGIVSV
                     EPGILGSQIFYVAGSGMQIFMSKKDFPDLKVGDRVQLTGELSQIQGETRLKIAAKEDI
                     ILIAQAEPPQPEEILTGDVSEELEGYLVTVEGELLETKSRIMYLDDGSEEVTVYVKSS
                     TGLPAPKYEPGTTLKISGIVSQTKTGYRILPRYASDIKVVSAAMDKAINETKLENSDM
                     KTRVMRYLIASIAASIIILIIIKLKSKNQNGK"
     gene            2175..2549
                     /locus_tag="VGA49_02755"
     CDS             2175..2549
                     /locus_tag="VGA49_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9664720.1"
                     /translation="MSNQIQNPKDKKYNLGERTANFGEDIINFCKRLENNVINRELIR
                     QLVRSATSIGANYAEADGAESKKDFKHKIAICKKESKETKHWIRMISAANPLFKNQCK
                     IFWQEAQEFTLIFSSIYNSNKK"
     gene            2736..3005
                     /gene="rpsT"
                     /locus_tag="VGA49_02760"
     CDS             2736..3005
                     /gene="rpsT"
                     /locus_tag="VGA49_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9664721.1"
                     /translation="MPVKKSAHKELRKAKKRTAHNLIYKRNLEYLTRQFKKAFQDKSA
                     AQLKELSNKIIKAIDKMAQKGILKKNTAARRKSRLMAKVNSLTKE"
     gene            complement(3006..>4065)
                     /gene="holA"
                     /locus_tag="VGA49_02765"
     CDS             complement(3006..>4065)
                     /gene="holA"
                     /locus_tag="VGA49_02765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9664722.1"
                     /translation="FGLDNFELISRNPQFYNSIIHTSVLIYLYGEDTFSSRQKLKQLK
                     DKFLKEVDPSGVNLVVLDGEKINIDQFNQAVLSVSFLSRRRMVVIENLLKSKDKKMAE
                     AVAGLIDKESVKDNILVFWEDQPADSKSGYKKKSPKEQANPLIKKLLKEKYVYRFQPL
                     NESQLHKWVNDEVKQSGLEIDNQAVKLLISYVGGNLWQLDREVKKLISYARAVTAKTI
                     LADYVQLLVPARYDDNIFNLIDALSQKNKKRASRLLRDQLDSGQNEMYLLTMIVRQFK
                     ILLQVKEILDKKPGYGHLASLLRIHPFVAQKAAGASKNFSLTQLKNIYQKLLEIDFKI
                     KTGWAKPELLLDLLVVGL"
BASE COUNT         1297 a          892 c          809 g         1067 t
ORIGIN      
        1 aaaaccagcc aaattctgaa actcccgtcc ggcaggcggc cgggtttagc gccggcgata
       61 tcttaattaa cgaattcgta gctgaccccg gtgataacga aaccgaatgg gttgaactaa
      121 ggaacatgac ccactcaatc attaatctgg aaagctggac agtggcagat ggctccggga
      181 aaattactga actggccggc ctgattgaac cggaaaatta ttttgtcata gaaaaaccaa
      241 aaggcgttct caataatacc ggcgatttaa taattttaaa atttaaagaa accgttattg
      301 accgggtggc ttacggcgac tgggatgatg gcgacatagc tgacaacgct ccaaaagcgg
      361 ctgatcccaa ttcagttgcc cggaccgatt ttaacgatgc cgaccacgac ttcaatgact
      421 ttcaggtgac caccacccct actaaaggag gaactaatat tattagtgct ccaataattt
      481 ctgaagaaaa aaataattct tcagaaactc tcaagactcc gccgccggtt gacccgacaa
      541 tctacaaaca tttgattatt aatgaattct tgcctaatcc cgccggcccg gacgccaccg
      601 atgaatttat tgagcttaaa aataacggtt caggcccggt tgacattgaa aacctgatta
      661 ttcaggacaa cagttccaaa aaacataaaa tcaatcaaaa agattttgat tccactttaa
      721 ttcagcccgg cggttttttt gtaatcaacc ggtcagtctc ggggattgcc ctcaataaca
      781 ccggcggtga cgccgtcaaa cttttcagtc ctgatgaact attaattgaa caaattgatt
      841 actttgaaaa agccgaagaa ggcctgtcat actcccgaga tcaggatgac gactggtttt
      901 ggacagcggc cattaccccc ggggcggcca acagcctgcc tgaattaata acaattgaag
      961 aaccaaatcc cggatcagcc gatgaatcaa tcgcgacggc tgaaccggcc gaaaaacaaa
     1021 cggactaccc gccaattatg atcaccgaag ttctgcccaa cccggccggc tctgattccg
     1081 gggagtggct ggaactatat aaccccaatg actttgagat tgatctgacc ggctggcgaa
     1141 tagatgatga agaaggcggc agccggcctc ataaaattaa agacacggtt atcggtccgc
     1201 gacaatattt agtcttaccc cgacagcaaa ccaaactggc tttaaataat tctggcgatt
     1261 cagtcagatt atttgatcct aataatacct taattgattt aacggactat gaagccgccc
     1321 gggaaaacag ttcctgggcc ttgtctgatg attatgaatg gagctggaca gccaatctga
     1381 ctccgcagga cgccaatatt tttacccagc cggccactgc cgctgataaa aaaaacaaaa
     1441 acaaacagat tattcagacc actttaaaac aagcccggga aaatgatccg ggggatttaa
     1501 ttcaggttga gggcatagtt tcagttgaac cgggaatttt aggctcgcag attttttacg
     1561 tggctggttc aggaatgcaa atttttatgt caaaaaaaga ttttcccgac ctgaaagtcg
     1621 gcgaccgggt tcagctaacc ggcgaattaa gccagattca gggagaaacc cggctaaaaa
     1681 tcgccgccaa agaagatatt atcctgattg cccaggctga accgccccag ccggaagaaa
     1741 ttttgaccgg cgatgttagt gaagagctgg aaggctattt agtgacggtt gaaggcgagc
     1801 tgctggaaac taaaagccgg ataatgtatc tggatgacgg ctcggaagaa gtgactgttt
     1861 atgtcaaatc atctaccggc ctgccggctc ccaaatacga gccgggaaca accctaaaaa
     1921 tttccggaat agtcagccaa accaaaaccg gctaccggat tctgccccgc tatgcttcag
     1981 atattaaggt tgtgtcagcg gccatggata aggcgattaa cgaaactaaa ttagaaaatt
     2041 ctgacatgaa aacccgggtt atgcgttatt taatagcttc aatcgccgcc agcatcatca
     2101 tcctgatcat aataaaatta aaaagcaaaa atcaaaatgg aaaatgatta ttaatttcaa
     2161 atttcaaaat ccaaatgtca aatcaaatcc aaaatccaaa ggacaaaaaa tataatcttg
     2221 gggaaagaac agctaatttt ggcgaagata tcatcaactt ttgtaagcgc ttagaaaaca
     2281 atgtcataaa tcgggaactg ataaggcaat tggtcagatc ggcaacaagt attggcgcca
     2341 attatgctga agccgatggc gctgaatcta aaaaagattt taaacataaa atagcaattt
     2401 gcaaaaaaga gtcaaaggaa accaaacact ggatcagaat gatctccgca gcaaatcctc
     2461 tattcaaaaa tcaatgtaaa attttttggc aggaagcaca agaattcact ttaatattct
     2521 cttcaatcta caattcgaat aaaaaataat ttgacattga gcttttgacc ttcaattgat
     2581 atttgacctt tgaaatttgg attttatgtt taaattttaa ctgtggtttt gcgttttgac
     2641 atttacattt tgcgttattt ccaactctct tgcaatcccc aatgaatcat gttatattta
     2701 tttcaattaa ataaattgaa ttttttatca cgtttatgcc tgtcaaaaaa tcagcccaca
     2761 aagaattaag aaaagccaaa aaaagaaccg cccacaatct catctacaag agaaatcttg
     2821 aatatttaac gaggcagttt aaaaaagctt ttcaggacaa atcagctgcc cagcttaagg
     2881 agctatccaa taaaataatc aaagccattg ataagatggc gcaaaagggt atcctcaaaa
     2941 aaaatacggc cgcccgccgc aaatccagat tgatggccaa agttaactct ttgactaaag
     3001 aataactaca agccgaccac caataaatca agtaacaact ccggcttagc ccagccggtt
     3061 tttattttaa aatctatttc cagcaatttt tgataaatat tcttcagctg ggtcagacta
     3121 aaatttttgg aagcgccggc cgctttttga gcgacaaacg gatgaatacg cagcaatgac
     3181 gccaggtgac cgtagcccgg ttttttatcc agtatctcct taacttgcag taaaatttta
     3241 aactgccgga caatcattgt cagcaaatac atttcattct gtccgctgtc cagctggtcg
     3301 cgcagaaggc ggctggctct ttttttgttt ttctggctca aagcgtcaat caaattaaaa
     3361 atattgtcat catagcgggc cggaaccagc agctggacat agtcagccaa aatagttttg
     3421 gcggtcaccg cccgggcgta actgattaat tttttgacct cccggtcaag ctgccagaga
     3481 ttaccgccaa cataactgat caggagctta acggcctgat tgtcaatttc cagaccagac
     3541 tgtttaactt catcgttaac ccatttgtgg agctgggact cattcaaagg ctggaaccgg
     3601 taaacatatt tttctttgag cagtttttta atcaggggat tggcctgttc ttttggcgac
     3661 tttttcttat agccgctctt tgaatcagcc ggctggtctt cccaaaaaac caaaatgtta
     3721 tctttgacgg attctttgtc aattaaaccg gcgacagctt cggccatttt tttatcttta
     3781 cttttcagta aattttcaat aaccaccatc cgccgccttg ataaaaacga caccgacaaa
     3841 actgcctggt taaactgatc aatatttatt ttttcaccat ccaagacaac caggttaact
     3901 ccggatggat caacctcttt aagaaactta tctttgagct gttttagttt ttggcgggaa
     3961 gaaaaagtat cttcaccgta taaataaatt agcaccgaag tgtggattat tgaattatag
     4021 aattgcggat tgcgactaat tagttcaaag ttatcaagtc caaag
//
LOCUS       DASWHZ010000134         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_630983, whole genome shotgun sequence.
ACCESSION   DASWHZ010000134 DASWHZ010000000
VERSION     DASWHZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630983"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1505
                     /locus_tag="VGA49_02770"
     CDS             <1..>1505
                     /locus_tag="VGA49_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664723.1"
                     /translation="EWRKEAKEITDQLENLKNKLTDSQREEQKLTDKISHFEKKLTEL
                     KNKIKLDLTKEPLNDIAKEILAIHDQQEKLIKKIAKITPDTLAKETDFIDQLKKEAEN
                     VNKKLKDLTSQIKEQTSAIDLEEKIKTSETSAIWQIQEDLNQALRQKEELARKINDLK
                     IDLKVLDNKKELTDRELAGLSADRTKAEAEIKQLSAVGGSASGGKTAKGDLKKFDQEN
                     QAIRDKIEKIDTQIKAIENKLNQFNQTEEKKKEQLFRLERQYQNVQHEMNQYYKSLND
                     IKVELARLETKKEDLEKEIRQEMGDLKHLIRDLRELPPDSREVKSDQLFSRIEKLKYQ
                     LELIGGIDPETMTEYQETKERHDFLTTQAADLEQAIDSLEKVIKELDDKIHQQFDSAF
                     AKINKQFEKYFRILFGGGKAKLTKIIEEPPRQEPGYSLADQELGQSAGETEQPEPVVQ
                     EQSISRARKYQKTAYKGIEIYASPPGKKLKGVSMLSGGERSLTALALISAI"
BASE COUNT          574 a          314 c          316 g          301 t
ORIGIN      
        1 aagaatggcg gaaagaagcc aaagaaatta ctgatcagct cgaaaattta aaaaataaac
       61 tgaccgacag tcaaagagaa gaacaaaaac taactgataa gatcagtcat tttgaaaaaa
      121 aactaacaga actgaaaaat aaaataaaac tggatttaac caaagaaccg ctcaatgaca
      181 tcgccaaaga aattctggct attcacgacc agcaggaaaa attaatcaaa aaaatcgcca
      241 aaattactcc ggacactctg gccaaagaaa ctgactttat tgaccagctt aaaaaagaag
      301 ccgaaaacgt caataaaaaa ttgaaagatt taaccagcca aattaaagaa caaacttcgg
      361 cgattgatct ggaagaaaaa atcaaaactt ctgaaacctc ggcaatctgg caaattcagg
      421 aggacttaaa ccaggctctg cgtcaaaaag aggaactggc ccggaaaatt aatgatttga
      481 aaattgatct gaaagttctg gacaataaga aagaactgac tgacagggaa ctggccggcc
      541 tgtccgctga cagaaccaaa gctgaagctg aaataaagca gctttccgcc gtaggcggat
      601 ccgcctctgg cggaaaaacc gccaaaggcg acctcaaaaa atttgatcag gaaaaccagg
      661 ctatccgaga taaaattgaa aagattgata cccagattaa agccattgaa aataaactta
      721 atcaattcaa ccagacagaa gaaaagaaaa aagaacagct tttccgactt gagcggcaat
      781 atcaaaatgt tcagcacgaa atgaatcaat attataaatc tctcaatgat attaaagttg
      841 agctggcccg gctggaaaca aaaaaagaag atctggaaaa agaaattagg caggaaatgg
      901 gcgatttgaa acatttaatc cgtgacttaa gggaactgcc tcctgattcg cgggaagtca
      961 aaagcgacca gcttttttcc cggattgaaa aactaaagta ccagctggag ctgattggcg
     1021 ggattgatcc cgaaaccatg acagaatacc aggaaaccaa agagcgtcat gattttttga
     1081 caacccaggc ggctgacctg gaacaagcca ttgattccct ggaaaaagtc attaaagaac
     1141 tggatgataa aattcatcag cagtttgatt cggcttttgc caaaatcaac aaacaatttg
     1201 aaaaatattt tagaattctt tttggcggcg gcaaagccaa actgactaaa atcattgaag
     1261 agccgccccg gcaggagccg ggctacagcc tggctgacca ggagctgggg cagtccgcgg
     1321 gtgaaactga acagcccgaa ccggttgttc aagaacagtc aatcagccgg gctagaaaat
     1381 atcaaaaaac agcctacaaa ggcattgaaa tttacgcttc cccgcccggc aaaaagctca
     1441 aaggcgtcag catgctgtcc ggtggcgaac ggtcgctaac cgccctggct ttaatttcag
     1501 ccatt
//
LOCUS       DASWHZ010000135         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_260410, whole genome shotgun sequence.
ACCESSION   DASWHZ010000135 DASWHZ010000000
VERSION     DASWHZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260410"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..673
                     /locus_tag="VGA49_02775"
     CDS             293..673
                     /locus_tag="VGA49_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9664724.1"
                     /translation="MKLKDKLIHPELSYAIVGVLYQVHNELGRYCGEKQYGDLIETKL
                     KKNLIQFKREKILDKMFSEEKVGRHRIDFLIEARVVLEIKCKRLLEKPDYYQVKRYLE
                     VLNFQLGLLVNFRERILKPRRILN"
     gene            765..>1816
                     /locus_tag="VGA49_02780"
     CDS             765..>1816
                     /locus_tag="VGA49_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX9664725.1"
                     /translation="MISKLYSAALIGLDSELIEVEADLISSYTPGIFIVGLPDKAVEE
                     SKERIRSAIKNSRLSLPRKRIVINLAPADLKKQGPAYDLPIAVSILLAQEEIKFNFDY
                     QKCLFVGELALDGRLRNVNGILSVASMAKDRGIKTLFLPKINAAEASLIPGLSIIPLN
                     NLEELILHLNQDKKIKPLTGRKSKLKIDFDNYHYDMAYIKGQEQAKRALEIAAAGGHN
                     LVMSGPPGSGKTLLARTMPSILPTMNLDEALEVTKIYSVAGLLPLSQPLLTSRPFRNP
                     HHSASAVSLVGGGSWPRPGEVSLSHRGVLFLDEFCEFPRSVLENLRQPLEDGFVTISR
                     AAGTSRFPARFILVAA"
BASE COUNT          543 a          374 c          413 g          486 t
ORIGIN      
        1 cagagttctt ccgggctgga ccgcaaatca atttcaaact gatactgaaa gacccgcctg
       61 aagagccagc gaaaaaaagc aaacatcatc cctccccggg gtttaattgt caggaacaaa
      121 gattaagcca cggctcaatc atcagagcat aaaagaaaat tgacagattg tcaacctgtg
      181 ttatactgta aatgatttcc acccctgacc ctggcattgc caaggccggg ggtattttat
      241 ttgaggtgtt atcctaatca acgaattcta ttctaattta caaataacta atatgaaact
      301 aaaagacaaa ttaattcatc cggaattgtc gtatgcaatt gttggtgttc tatatcaggt
      361 tcataatgag ctgggaagat attgtggtga aaaacagtat ggggatttga tagaaacaaa
      421 attaaaaaaa aatttaattc agtttaagcg ggaaaaaatt ttagacaaaa tgttttcaga
      481 agaaaaagtc ggacgccatc gcatagattt tctgatagaa gctagggtgg tactggaaat
      541 taaatgtaaa cgacttttag aaaagccaga ttattatcaa gttaaaagat accttgaggt
      601 tttaaatttt caattagggc tgctcgttaa ctttcgcgag aggatattaa aaccgagacg
      661 cattttgaat tgaaggtctg ttgtaaatat ttgtaaaaaa tatattagtg attagttgat
      721 taggataaaa tttgtaaatt aggataacat taatcttatc atttatgatc tccaaactct
      781 actccgccgc cctgatcggc ctggatagcg agctgattga agttgaagct gatttgattt
      841 cttcttacac gccgggaatt tttattgtcg gtctgccgga caaagcggtt gaagaatcca
      901 aagaaagaat ccggagcgcc attaaaaaca gccggttgtc attgccccgg aaaagaattg
      961 tcattaatct ggcgccggct gatttaaaaa agcaggggcc ggcttatgat ctgccgatcg
     1021 ccgtcagtat tttactggcc caggaagaaa taaaatttaa ttttgactac caaaaatgtt
     1081 tgtttgtcgg cgaactggct ctggacggcc gattaaggaa tgttaacggg atattgtcag
     1141 tcgcttccat ggccaaagac cgcggcatta aaactttatt tctgccaaaa attaacgccg
     1201 ctgaagccag cctgattccg ggattatcaa ttatcccctt aaataatctg gaagaactga
     1261 ttctgcatct taatcaggat aaaaaaataa aacctttgac cggtagaaaa tcaaaactta
     1321 aaattgattt tgataattat cactacgaca tggcctacat caaaggccag gagcaggcca
     1381 agcgggcttt ggaaatcgcc gccgccggcg gtcacaatct ggtgatgtcc ggcccgcccg
     1441 gctcgggaaa aactttgctg gcccggacca tgccgtcaat tctgccgaca atgaatctgg
     1501 acgaggccct ggaagtcacc aaaatttaca gcgtcgccgg attattgccg ctaagccagc
     1561 cgctattgac cagccggccg tttagaaatc ctcatcacag cgcttcggcg gtctcactgg
     1621 ttggcggcgg atcgtggccc cggccgggag aggtttccct gagccaccgg ggagtgttat
     1681 tcctggatga attttgtgaa tttccccggt cagtcctgga aaatttaaga cagccgctgg
     1741 aagacgggtt tgtgacaatt tcccgggcgg ccggcacttc ccggtttcca gccagattta
     1801 ttttggtggc ggccat
//
LOCUS       DASWHZ010000136         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2863569, whole genome shotgun sequence.
ACCESSION   DASWHZ010000136 DASWHZ010000000
VERSION     DASWHZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2863569"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGA49_02785"
     CDS             <1..346
                     /locus_tag="VGA49_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664726.1"
                     /translation="IHNMLISQGILTGSKKNVSSLGKKFKEKMTKKEDGQESGQQPTV
                     NGQQGSQQEKEKPAEPVKAEEAKPEKPETKEPKTEELKPEEPRAEEKSEEAKPEVEAL
                     KEEPKAEENPAS"
     gene            complement(581..1798)
                     /gene="tyrS"
                     /locus_tag="VGA49_02790"
     CDS             complement(581..1798)
                     /gene="tyrS"
                     /locus_tag="VGA49_02790"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9664727.1"
                     /translation="MAIKTPGQQKLKELLTRGVEEIIAEEELEKKLRKGEQIRIYYGI
                     DPTSVELHIGHAVILRKLKEFQALGHEVILLIGDFTAQIGDPSGRTIQRVPLTHKQVL
                     DNAKTYQEQASKILDFESKSNPVKIKFNGHWLQKLTFKELIELSMLFTVQQFLERDMF
                     QDRLQAGKPIGLHEFLYPLMQGYDSLAMDVDMEVAGSDQLFNMLIGRDLLGKLKNKSK
                     IVMTFELLEGLDGRKMSKTFNNFVGLADEPSDMYGKIMSMKDELITRYFKLCTDVSLD
                     EIKKMDRQIAAGGNPRDFKMKLAWELVKTYYSVPDANSAEKNFINVFQKKKKPEEMEE
                     FKIQIGRHNIVDLLVNTELVSSKSEARRVIEQGGVKVDDKVIDDPTEDIEVGEKGVVM
                     QKGKLGYVRVVGE"
BASE COUNT          569 a          447 c          344 g          573 t
ORIGIN      
        1 catccacaat atgctgatta gccagggcat tttaaccgga tctaaaaaaa atgtttcttc
       61 tttaggcaaa aaatttaagg aaaaaatgac taaaaaagaa gacggtcagg agagcggtca
      121 acagccaaca gtcaacggtc aacagggcag tcaacaggaa aaagaaaaac ccgctgaacc
      181 ggtaaaagca gaggaagcta agccagagaa accagaaact aaagaaccaa agactgagga
      241 actaaaacca gaagaaccca gggcagagga aaaatcagaa gaagctaagc ctgaagtcga
      301 agcgctaaag gaagaaccaa aagcagagga aaacccagct tcataatttt caaatagctc
      361 aaacaaaaaa agctctcgcc cccgagagtc tttttaattt ataaatcctg acatccaata
      421 gtagaaatcc tatttttaaa attcgaatat ctaaatccga aatactaaat aaatcataaa
      481 ttataatttt cgaaattcta aacaatcagt attgattgtt tggaacattg taatttatta
      541 tttcgaattt atttaggatt tcgatattag gatttcgaat ttattccccg actaccctca
      601 cataccccag cttccctttc tgcatcacca cgcccttctc tcccacctca atgtcttccg
      661 ttggatcatc aatcactttg tcatcaacct tgactccacc ctgctcaatc acccggcggg
      721 cctcgctttt ggaagacacc agttcagtgt taaccagcaa atcaacaata ttatgccggc
      781 cgatctgtat tttaaattct tccatttcct ccggtttttt ctttttttga aaaacattga
      841 taaaattctt ttcggccgaa ttggcgtccg ggactgaata ataggttttg accagctccc
      901 aggccagctt cattttaaaa tcacggggat tcccgccggc ggcaatttgc ctgtccatct
      961 ttttaatttc atccagacta acatcggtgc agagtttgaa atacctggta atcagctcat
     1021 ctttcattga cattatttta ccgtacatgt cgctgggctc gtcagccagg ccgacaaaat
     1081 tattaaaagt cttggacatt ttccggccat ccaggccttc caataattca aaagtcatca
     1141 caattttgga tttatttttt aatttgccaa gcaagtccct gccaatcagc atattaaaca
     1201 gctgatccga gccggcgacc tccatgtcaa catccatcgc caggctgtca tagccctgca
     1261 tcaaaggata taaaaattca tgaaggccaa tcggcttgcc cgcctggagg cgatcctgaa
     1321 acatatcccg ttccaaaaac tgctggactg taaacagcat tgacagctca attagttctt
     1381 taaatgtcaa cttttgcagc cagtggccgt tgaattttat tttaaccgga ttgctttttg
     1441 attcaaaatc caaaattttt gaagcttgct cctgataagt tttggcgtta tccaaaacct
     1501 gcttatgcgt cagcggcacc cgctgaatcg tccggccgct ggggtcgccg atctgggcgg
     1561 taaaatctcc aattaacaaa atgacttcat gccccagcgc ctgaaattct ttcagtttcc
     1621 tcaaaatgac ggcgtggccg atatgaagct ccacgctggt cggatcaatt ccgtaataaa
     1681 ttctgatttg ctcgcctttt cttaattttt tttccagttc ttcttcggcg ataatttctt
     1741 caacgccgcg ggttaataat tcttttaatt tctgctgtcc gggagtttta atggccataa
     1801 ttttttaagt caataatttc agctgttttt ccaaattggt taatttttct ttaaactggt
     1861 ttaatttctg ccggtcagcc tcaacaattt cctggggcgc ctggctggta aattttttat
     1921 tttttagctt ttt
//
LOCUS       DASWHZ010000137         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2495821, whole genome shotgun sequence.
ACCESSION   DASWHZ010000137 DASWHZ010000000
VERSION     DASWHZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2495821"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="lspA"
                     /locus_tag="VGA49_02795"
     CDS             complement(<1..587)
                     /gene="lspA"
                     /locus_tag="VGA49_02795"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9664728.1"
                     /translation="MNNRAVPPWRDGRRLFTPPSAGFIICTPLHPRGKPRGIRGQIIK
                     AVSFDILFLLTVIVDQFIKNYFLKNPEVRDFLIETGFLKLTARFNQNYNLAFGIPLPR
                     LLIISLMTAAIIILFSLLVKKLLAGRLFETVVILLIIYGAVSNLIDRIWHGFVIDYID
                     LIIVSVSWPVFNLADLMIVTGIILWLSYDLKKSPPP"
     gene            complement(606..986)
                     /locus_tag="VGA49_02800"
     CDS             complement(606..986)
                     /locus_tag="VGA49_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX9664729.1"
                     /translation="MNKKTLNLIKEKLLKEKARLAKELGQFASESKKVKHDYDADFPD
                     MGDKEDENALEVAAYGDRLTLERTLEKKLQDVVSALEKIEKGQYGVCKYCGQEIDEKR
                     LLARPESGACVSCKAELQSRPKIV"
     gene            complement(1582..>2607)
                     /locus_tag="VGA49_02805"
     CDS             complement(1582..>2607)
                     /locus_tag="VGA49_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664730.1"
                     /translation="TPALAAISDSADALNLLTYQNKLPNLKQARDFKVKRFYFGSIAQ
                     AYINGRQAGRLSQHLNDQPPAQMFLTALTQNQRQDLYISLNKSSQRILIEISGLDQDF
                     INSLNRRPLLVNYLPPGSVATLASANLQKSFNSWQRQLAFINPELKNYLDQLTDDFER
                     TKNFQFQTERLLSAVNGPAELILLDKNNFVLILTNQFPRFSDIEETVRSVMAVNFPVS
                     QPKLLPDGTEVIELVADPTTILTEQSKLNRPEALDIYKINPPESEFQLIFGYSPKLVF
                     ITNKIDNIIQILEKNQPACVQSPPRLLEELMVLGQPILKTLPYAQGLQCVIIEQNNYQ
                     TNLELIY"
BASE COUNT          773 a          560 c          513 g          761 t
ORIGIN      
        1 gggggggggg atttttttaa atcgtaactt agccagagaa taatgccggt aacaatcatc
       61 aagtcagcca aattgaaaac cggccagctt accgaaacga ttattaaatc aatataatca
      121 atcacaaaac catgccaaat ccggtcaatc aaattgctga cggcgccgta aataattaaa
      181 agaataacga ccgtttcaaa taaacggccg gctaataatt tttttaccag taaagaaaaa
      241 aggataatga ttgccgccgt catcaggctg ataatcagca accggggcaa agggatgcca
      301 aatgccaaat tataattctg gttaaaccgg gccgttaatt ttaaaaaacc agtctcaatc
      361 aaaaaatccc ggacttcggg attttttaaa aaataatttt ttataaattg atcaacaatg
      421 acagtcagca ggaataaaat atcaaaacta accgctttta ttatctgccc gcgaataccc
      481 cgcggcttgc cgcggggatg aagcggcgtg cagataataa accccgccga cggcggggtg
      541 aataaccgcc tgccgtcccg ccaaggcggg acggcgcggt tattcatcaa aaaatttttt
      601 ttcatctaaa caatcttagg ccggctttgc aattcagcct tgcaggaaac gcaggcgccg
      661 gactccggcc gggccagcag tcgcttctcg tcaatttctt ggccacaata tttgcagacg
      721 ccgtattggc ctttttcaat tttttccagc gcgctaacca cgtcctgcag ttttttctcg
      781 agggttcttt ccaaagtcag ccggtcgccg taggcggcca cttccagagc gttttcatct
      841 tccttgtcgc ccatatccgg gaaatcagcg tcataatcat gtttaacttt tttactttca
      901 ctggcaaact ggccaagctc tttagccagc cgggcttttt ccttgagtaa tttctcttta
      961 attaaattta aagtcttttt attcatactc ttttatataa taaaacctta ttataaaata
     1021 cccaaaaaat caagtttttt caaaaaaaag gaggcgccag gtttagcgtc ttgacaattg
     1081 ttgctattac gcttctggcg ccctttcttc gagcgccact tgtttccgct ttcttttagc
     1141 ggatagaagt atagcgctca aagacaacaa agagctcgct ctcgaccatt cgttggcgaa
     1201 gattgctttg cggcaacttc gccttttttt tcttttgttt ttgtttttgt gtctctcctc
     1261 gagacttaac ctgcgaccta atcgggttag ttggttaggg ttagttggct accctaaccc
     1321 attaaaaaaa cgaatagttg agagaatatt agctaaaaaa cctactctgg cttttaacca
     1381 acggttctcc cggctattcg catgcttcgt aacttaataa tatcacccta tttggcaaat
     1441 gtcaagcttt agcctgatat taaagctaaa gttagcaaaa aaatattcat tatttgtaaa
     1501 ttaagataag atttgtaaat tcgtataaca ttataaatat ccacaaacta tccacatatc
     1561 aacaaaaatc ccctaaatta tctaataaat aagctctaaa ttggtttgat agttattttg
     1621 ctcaattatt acgcactgca gaccctgtgc ataaggtaaa gttttcaaaa ttggctgccc
     1681 caataccatt agttcctcta acagccgggg tggagactga acgcaggccg gctgattttt
     1741 ttctaaaatt tgaataatat tatcaatttt gttagtaata aaaactaatt ttggactata
     1801 gccgaaaatt aactgaaatt cactttcagg cggattgatc ttataaatat caagggcttc
     1861 aggtcggttt agcttagact gctctgtcaa aatagtggtt ggatcagcga ccagctcaat
     1921 gacttcagtg ccatcaggca ggagcttggg ctgactgacc gggaaattaa cagccataac
     1981 gctcctgaca gtttcttcaa tgtcagagaa tcgcggaaat tggttcgtca aaattaaaac
     2041 aaaattattt ttatccagca agatcagctc ggccgggccg ttaacggctg acaacaaacg
     2101 ctctgtctga aactgaaagt ttttagttct ttcaaaatcg tctgtcagct gatccagata
     2161 attcttaagc tccggattta taaaagccag ctgtctctgc cagctattaa aacttttttg
     2221 aagattagcg ctggccagcg tcgcgactga accgggcggt aaataattaa ccagtaaggg
     2281 ccggcggttg agagaattaa taaaatcctg atcaagaccg gaaatttcaa ttaaaatcct
     2341 ttgagaggat ttgtttaaac taatataaag atcctgccgc tggttttgag tcaaagccgt
     2401 caaaaacatt tgagccggcg gctgatcatt taaatgctgg ctcagccggc cggcctgccg
     2461 accgttaata taagcctggg cgattgatcc aaaataaaat cttttgactt tgaaatctct
     2521 ggcctgtttt aaattaggca gtttgttttg atatgtcagt aaattcaaag cgtcggctga
     2581 atcagaaata gccgccaggg cgggagt
//
LOCUS       DASWHZ010000138         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_635400, whole genome shotgun sequence.
ACCESSION   DASWHZ010000138 DASWHZ010000000
VERSION     DASWHZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635400"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /gene="dnaK"
                     /locus_tag="VGA49_02810"
     CDS             <1..1562
                     /gene="dnaK"
                     /locus_tag="VGA49_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9664731.1"
                     /translation="AEEKLGDKITEAIITVPAYFDDAQRQATKDAGKIAGLEVKRIIN
                     EPTAAALAYGFDKRKGQQVVVYDLGGGTFDVSVLDIGEDVVEVKSTAGDTHLGGDDID
                     QVVINWILAEFKKDQGIDLSNDQLALQRLKEAAEKAKIELSSSLETEINQPYITSDAG
                     GPKHLLLKISRARLEELVGDLIQKTLEPCQQALKDAGLSAKDIEEVILVGGQTRMPLV
                     QQAVEKFFGRKPNVSVNPDEVVALGAAVQAGVLQGEVKDVLLLDVTPLSFGIETLGGV
                     MTKLIERNTTIPTSKSQVFSTAADNQTSVEIHVLQGERGMAQDNKTLGRFILSGIPPA
                     PRGIPQVEVSFDIDANGILNVSAKDKATGKEQKITITASSGLSKDEVEKMQKDAELHE
                     EDDKKKKELIETRNLADTMVYTTEKMLKEAGDKVTADEKKDIEDKLAAVKTVKDGDDL
                     EVIKKASDDLSQAAQKVGAKMYQEQPTANSQQPTGEGTGAEERSAKGGSASGGKETVE
                     GEYEEVDKDKK"
     gene            1650..>2691
                     /gene="dnaJ"
                     /locus_tag="VGA49_02815"
     CDS             1650..>2691
                     /gene="dnaJ"
                     /locus_tag="VGA49_02815"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9664732.1"
                     /translation="MSKDYYNVLGVDRNSNQDEIKRAFRKKAHQYHPDKKDGDETKFK
                     EVSEAYQVLSNAEKRQQYDQFGTTFDQGQGGFSGFDFNQGFQGFDFSNFGDIFEGMFG
                     GFTGGRRRAGPVRGSDIQTELELNFREAVFGTDKTIELYKTDKCDHCQGNGAEPGTKI
                     ITCSTCGGSGQVASVQRTVLGNFQTVKTCSDCRGEGKKAEQACSKCQGRGIIKAGKKI
                     KVKIPAGIDKGEVIRISGEGQAGLRGGPNGDLYIEARVKPDPEFKRTGQDIISKIKLS
                     FPLAALGGKIKTDTLHGEVMLKIPAGIQSGQTIKLKGKGVPKLHGSGHGDHLVKVEVT
                     TPEKLSAKGKKLL"
BASE COUNT          832 a          521 c          710 g          628 t
ORIGIN      
        1 acgctgaaga aaagctaggc gataaaatta ctgaagccat catcacagtg ccggcttatt
       61 ttgacgacgc tcagcggcag gccaccaaag acgccggcaa gatcgccgga ctggaagtta
      121 aaagaatcat taatgaacca acagcggccg ccctggctta tggttttgac aaaagaaaag
      181 gccagcaggt ggtggtttat gacctgggcg gcgggacttt tgatgtttca gttctggaca
      241 ttggcgaaga tgtcgtggaa gttaaatcaa ccgccggtga tacccatttg ggcggcgatg
      301 atattgacca ggtggttatt aactggatct tggcggaatt taaaaaggat cagggaattg
      361 atctttcaaa cgatcagctg gcgttacagc gactgaaaga agccgctgaa aaagccaaga
      421 ttgagctgtc ttcatccctt gaaacagaaa ttaaccagcc ttatattact tcagacgctg
      481 gcgggccgaa acatttactg ctgaagataa gccgggccag gctggaagaa ctggtcggtg
      541 atttgattca gaagaccctg gagccctgcc agcaggcttt gaaagacgcc ggtttgtcag
      601 ccaaagatat tgaagaagtt attttagtcg gcggccagac cagaatgcct ttggttcagc
      661 aggccgttga aaaatttttt ggcagaaagc cgaatgtctc ggttaatccg gacgaagttg
      721 tcgctttggg ggcggctgtt caggccggag tgttgcaggg tgaagtcaaa gatgttttat
      781 tgctggatgt tactccgtta agttttggaa ttgaaactct gggcggcgtg atgactaaat
      841 taattgagag gaatacgact attccaacct caaagagtca ggttttttcc acggcggctg
      901 ataaccagac ttccgttgaa attcatgttt tgcagggcga gcgggggatg gcccaggaca
      961 acaagacttt gggacggttt atcttatccg gcattccgcc ggcgccgcgc ggcattcccc
     1021 aggtggaagt ttcttttgac attgacgcca acggtatttt aaatgtgtcc gccaaagaca
     1081 aagccaccgg caaagagcag aagattacga ttaccgcttc atccggctta tccaaggatg
     1141 aagttgaaaa aatgcaaaaa gacgccgagt tgcacgaaga ggatgataaa aagaaaaaag
     1201 aactgattga aaccagaaat ctggccgaca ccatggttta tacaacagaa aaaatgctga
     1261 aagaagcagg cgataaagta acggctgatg aaaaaaaaga cattgaagat aagctggcag
     1321 ccgttaaaac agtcaaagac ggcgatgatc tggaagtcat taaaaaagcg tcggatgacc
     1381 tgtcccaggc ggctcaaaaa gttggggcaa agatgtatca ggaacagcca acagccaaca
     1441 gccaacagcc aacaggggag ggaacaggag ctgaagaaag gtccgccaaa ggcggatccg
     1501 cctccggcgg aaaagaaacc gttgaaggag agtatgagga agtggataaa gacaaaaagt
     1561 aaaattcaaa atttaaaatt acaaatccta aataagcacc aattacaaaa ttataaattc
     1621 aaaagatttg gtgcttttac tataaaacga tgtctaaaga ctactacaat gtcctcggtg
     1681 ttgatcgtaa ctctaatcag gatgagatta aaagagcttt ccggaaaaaa gctcatcagt
     1741 atcatcctga caaaaaagac ggtgatgaaa ctaagtttaa agaggtcagt gaagcttatc
     1801 aggttttaag caacgccgaa aaacgacaac agtatgatca gtttggaacc acctttgatc
     1861 agggtcaggg tggtttttcg ggttttgatt ttaaccaggg ttttcagggt tttgatttca
     1921 gcaattttgg cgatattttt gaagggatgt ttggcgggtt tacgggcggc cgccggcgag
     1981 ccggcccggt cagaggatct gacattcaga ctgaactgga attgaatttc agggaagcgg
     2041 tttttggaac tgataaaacg attgaacttt ataaaactga taaatgcgac cattgccagg
     2101 gcaatggagc cgaaccgggc accaaaatta ttacctgctc tacctgcggc gggtcaggcc
     2161 aggtggcttc cgttcaaaga actgttttgg gtaattttca gacggtaaaa acctgttcag
     2221 actgccgggg tgaaggtaaa aaggctgaac aggcctgctc taaatgtcag gggcggggga
     2281 ttattaaagc cggcaaaaaa attaaagtta aaattccggc cgggattgat aagggcgaag
     2341 ttatcagaat ttctggtgaa ggccaggctg gtctccgggg cggtccaaat ggtgatttat
     2401 atattgaagc cagggtcaag cctgatccgg aatttaaaag gacgggtcag gatattattt
     2461 caaaaattaa actttcattt cctttggcgg ctcttggcgg taaaattaag acagatactt
     2521 tgcatggcga agtaatgctt aaaattccgg ccggaattca gagcggccag acgattaaac
     2581 taaaaggcaa aggcgtgcct aaattgcatg gttcaggcca tggcgatcat ctggttaaag
     2641 ttgaagtaac cactcctgaa aagttatcag ccaaaggaaa aaaactcctg g
//
LOCUS       DASWHZ010000139         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2497892, whole genome shotgun sequence.
ACCESSION   DASWHZ010000139 DASWHZ010000000
VERSION     DASWHZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497892"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1392)
                     /locus_tag="VGA49_02820"
     CDS             complement(175..1392)
                     /locus_tag="VGA49_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9664733.1"
                     /translation="MNYYYEAKTRRGQAKKGYLEAKNKKAVLARLKDEKLVPVKIKID
                     WKSVLNLNILSFGYVPYVDKLMFTKHLSVLVGAGIPINEAISILIDQAASGGLKRILR
                     KVLVAVEKGQSLADGLANYPRVFPEIYVNIVRAGEKGGTLEASLNQLAIQLEKEFELR
                     SKIRSAMFYPILVLVAASVIGLSLAIFVLPRITSLFVGFNIELPLLTKILIALAGFFS
                     AYGGYVFVILAVLAVGLFWLLRTGPVRPVTHRLILRGFFIKTIAKDKNLALFSRTLGT
                     LLKSGLPIIEALELTTKTFNNVAYKAVVKKAVKEVEAGTPLAVSLQAYPNYFPPVVYR
                     MIGVGEQSGNLEQVLVYLAEFFEGEVDVKTKNLSTVIEPVLLLVIGLFVALLAVAIIS
                     PIYKITGSIGRGR"
     gene            complement(1404..>2320)
                     /locus_tag="VGA49_02825"
     CDS             complement(1404..>2320)
                     /locus_tag="VGA49_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX9664734.1"
                     /translation="RVSIVPVVTGEKVVMRLLAEKVRQFTLADLGLSAREYEKVSAAI
                     KKPYGMMLATGPTGSGKTTTLYAILKILNKREVNIATIEDPVEYDVEGVNQIQVNPKT
                     NLTFASGLRSIIRQDPDIIMVGEIRDQETAGIAINAAMTGHLVLSTLHTNDAATSLPR
                     LIDMEVEPFLIASTINVVIAQRLVRKICPTCVESYLVTDKDLEKLKKIIPIEKFLKGK
                     KIQKARLYKGKTCQVCGHSGYLGRIGLFEVMEITDNIRELIMRNADADTIKKQAVANG
                     MTTMLEDGIDKVLKGITTVDEVLRVALD"
BASE COUNT          619 a          591 c          479 g          631 t
ORIGIN      
        1 tctcccgatg gcgaagccat cgaggatcct cgacaaagtc gggacaaagt ccaaaaattt
       61 tgacatttgt catttggatt tcatttgagc tttaaacttt gatatttgac atttattatc
      121 attccctctc atttcttctc attccctctg attccttctg attccacttg gttttcatct
      181 cccccgtcct atactcccgg ttatcttgta tatcggcgag ataatcgcca cggccaataa
      241 agccacgaac aagccgataa ctaaaagcag aaccggctca atgaccgttg acaggttttt
      301 ggttttaacg tcaacctcgc cttcaaaaaa ttccgccaaa taaaccaaaa cctgctccag
      361 attgcccgac tgctcgccga cgccgatcat ccggtaaacg accggcggga aataattggg
      421 ataggcctgc aaagaaacag ctagcggcgt tccggcttca acttctttga ccgccttttt
      481 tacgactgcc ttgtaagcaa cattattaaa agttttggtt gttaactcca aagcttcaat
      541 aatcggcagg ccgctcttta aaagagtccc caaagtccgg ctgaataaag ccaggttttt
      601 atctttggcg attgttttta taaaaaaacc tcttaagatt aaacgatggg ttaccggcct
      661 gaccggcccg gtccgcagga gccagaacag cccgacagcc aacaccgcca agatgacaaa
      721 aacataaccg ccgtaagccg agaaaaatcc agccagagca atcaatattt tagtcaataa
      781 aggcagttca atgttaaaac caacaaacaa gctggtaatc cggggcaaaa caaaaatggc
      841 cagcgacaag ccaataacgc tggcggcgac taaaaccaaa atcggataaa acatggctga
      901 tctgattttt gatcttagct caaattcttt ttccagctga atggccagct gatttaagct
      961 tgcttccagc gtgccgccct tttcaccggc ccggacgatg ttaacgtaaa tttccgggaa
     1021 aacccgggga taattagcca ggccatcagc caaactctgt cctttttcaa ccgcgaccaa
     1081 aacttttctt aaaattcttt ttaaaccacc cgaggccgcc tgatctatca aaatagaaat
     1141 cgcttcatta atcggaatgc cggctccgac caaaactgac aaatgcttgg tgaacatcag
     1201 cttgtcaaca tagggcacgt agccaaaaga caaaatattc agatttaaaa ctgatttcca
     1261 gtcaattttg attttaaccg gcaccaattt ttcatctttc agccgggcca gaaccgcttt
     1321 tttatttttg gcttcaagat agcctttttt agcctgaccc cgccgggttt tggcttcgta
     1381 gtaatagttc attttggttt ttgttaatcc aaggccactc ttaaaacttc atcaaccgtt
     1441 gtaataccct tcaggacttt atcaattccg tcttccagca tggtcgtcat cccgttggcg
     1501 accgcctgtt ttttaatggt gtcggcgtca gcgttccgca taatcagctc ccggatgttg
     1561 tcggttattt ccattacttc aaaaagtcca atccggccca gataaccgga atggccgcaa
     1621 acctggcaag ttttcccttt atacaaacgg gctttttgga tttttttacc ctttaaaaac
     1681 ttttcaattg gaatgatttt cttgagcttc tccaaatctt tatcagtcac cagatagctc
     1741 tccacgcagg ttggacaaat tttcctgacc agccgctggg cgatgacgac attaatggtg
     1801 gacgcgatca aaaaaggctc aacttccatg tcaatcagcc ggggcaggct ggtggcggcg
     1861 tcgttggtgt gcaaagtgga caaaaccaga tggccggtca tggcggcgtt aatggcgatg
     1921 ccggcggttt cctgatcccg gatttcccca accatgataa tgtcggggtc ctgccgaata
     1981 attgaccgga ggccggaagc gaaagtcaaa ttggtcttgg gattaacctg aatttggtta
     2041 acgccctcaa catcgtattc caccggatct tcaatggtgg cgatattgac ttcccgcttg
     2101 tttaaaattt ttaaaatcgc gtaaagagtg gtcgttttcc cggaaccggt cggtccggtg
     2161 gccaacatca ttccgtaagg ttttttaatg gcggcggaaa ctttttcata ttcccgggct
     2221 gacagcccca gatcagccag agtaaactgc cggacttttt cagccagcag tcgcatgacg
     2281 actttttcac cggtaaccac cggcacgatt gaaacccgca
//
LOCUS       DASWHZ010000140         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2137817, whole genome shotgun sequence.
ACCESSION   DASWHZ010000140 DASWHZ010000000
VERSION     DASWHZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137817"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1654
                     /locus_tag="VGA49_02830"
     CDS             158..1654
                     /locus_tag="VGA49_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9664735.1"
                     /translation="MNSRKHQLFKLSLILIIILELLSLTGHLVNVVNQAVFLVLLLIV
                     LILSLKKLEYGLYFIVAELIIGSQGYLFSFDVNGLHISLRIGLFIIIMSVWLVKLLKA
                     RKLELRKSNLFWFYAALFIAIIWGAINGFLKDNSLQNLFFDANGYLFLLMALPFYAAI
                     NNLDQIKRLLEVGAAAVVASVIKVFITLYLFSHFSNELIVVMYKWIRDSRVGEITLVP
                     GGFFRIFFQSQLYAIIAFFIILAFLIRYIYPRIKGKIKIDRHQSVLLVFATVALTSAL
                     ISFSRSFWAAMMAAGGVLIIFVLIKYKNYFFSFLKSLVLFSALLFLSVGLMSLIIRFP
                     VPKTDLSLSAFGLLQARALALSGEAAVSSRWNLLPVLWEAITIHPVLGSGFGTTVTYQ
                     TADPRILALSPSGDYTTYAFEWGYLDIWLKLGLIGLMIYLALLFKILINGFKLLKNKA
                     DHFETSLVLGLFLSLIFLIVTHNFTPYLNHPLGISYLLLTARIFDILA"
     gene            1717..2076
                     /locus_tag="VGA49_02835"
     CDS             1717..2076
                     /locus_tag="VGA49_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9664736.1"
                     /translation="MTTKSTTLRATEARRKFFEILKDIDKLNRVYTITLDGRLKAVIL
                     SVDEYESWLETMDIMKNSKLMKDIKESAKKFKKGEYQTLEEVLAEEGFVAINKTDQYE
                     ISSDPGKKGRKKSKKNR"
BASE COUNT          611 a          342 c          472 g          780 t
ORIGIN      
        1 tttaaacagc cccgcccccg ggcttataat gaaattaatg gaattgagga agaagttaac
       61 gtttcatatt taatattatc agataattct tatagtttta ttgtgactga ttataacgac
      121 tctgaactgt taatcattga agcggatctt taacctgatg aattcgcgaa aacaccaact
      181 ttttaaactg tctttgatat taataattat tttggaactg ctttcgttga ccggccattt
      241 ggtaaacgtg gtcaatcagg cggttttttt agttttactg ctgattgttt taattttaag
      301 cttaaaaaag ctggagtacg gcctttattt catagtggcg gaattaatta ttgggtcgca
      361 gggatatcta ttttcttttg atgttaacgg gctgcatatt tctttaagaa ttggcttatt
      421 tattataata atgtcagtct ggctggttaa gcttttgaaa gccagaaaat tggaactacg
      481 caaatcaaat ttattttggt tttatgccgc gctgttcatc gccattatct ggggggcgat
      541 taatggtttt ttgaaagata atagtttgca gaatcttttt tttgacgcca acggctattt
      601 atttttactg atggctttgc ctttttatgc ggccatcaat aatcttgatc agattaaaag
      661 actattggag gttggcgcgg cggccgtggt cgccagcgtt atcaaagtgt ttataacttt
      721 gtatttattt tcccatttct caaatgaatt gatagttgtt atgtataaat ggatcaggga
      781 cagccgggtg ggggaaatca ccctggtgcc cggcggtttt ttcaggatat tttttcagag
      841 ccagctttac gcgataattg ctttttttat aattttagct tttttaatta ggtatattta
      901 tccgcggatt aagggaaaaa ttaaaattga ccgtcatcaa agcgttttgt tggttttcgc
      961 gaccgttgcc ttaacctcag ctttaatcag tttttccaga agcttttggg cggcgatgat
     1021 ggcggccggc ggggttttaa tcatattcgt tttaattaaa tataaaaact attttttttc
     1081 ttttttgaaa agtctggttt tgttttccgc tttgttgttt ttaagcgttg ggttaatgtc
     1141 tctgattatc cggtttccag tacctaaaac cgacttgagt ttgtcagctt ttggcttgct
     1201 tcaggcaaga gctttggctt taagcgggga agcggctgtt tccagccgct ggaatttact
     1261 gcctgttttg tgggaggcta ttacaattca tccggttttg ggttccggtt ttggaacaac
     1321 cgtcacttat cagacggctg atcccagaat tttggctttg agcccgtcag gcgattacac
     1381 gacttacgct tttgagtggg gttatctgga tatttggctg aaactgggtt tgattggttt
     1441 aatgatttat ctggctttgc tatttaaaat tctgatcaat ggttttaaat tgttaaaaaa
     1501 taaagctgac cattttgaaa ccagtttggt tttaggttta tttttatctt taatattttt
     1561 aatcgtgacg cataatttta ctccctattt aaaccacccc ttgggaatta gttatttatt
     1621 attgaccgcc cggatttttg atatcctggc ctgatattga caaagaattt aacgtacact
     1681 attatgtaca tagataatca ttaaaacatg aaatatatga ccaccaaaag cacgacctta
     1741 agggcgactg aagccagaag gaaatttttt gagatattaa aagatattga taagcttaat
     1801 cgggtttata cgattaccct ggacgggcgg ctcaaggctg taattttatc tgttgatgaa
     1861 tatgaaagct ggctggagac aatggatatt atgaaaaatt caaagttgat gaaggatatc
     1921 aaagagtcag cgaaaaaatt taaaaaaggg gagtatcaaa ccctggaaga ggttttggcg
     1981 gaagaaggct ttgtggcgat aaataaaact gatcaatatg aaatatcaag tgatcctggc
     2041 aaaaaaggcc gtaaaaaatc taagaaaaat agataaaaga tacaagggac gaattttaac
     2101 ggctttggtt agattaagca gtgagcccta tttaggaaaa tcacttgttg gtcagttgag
     2161 cggcaggttt tcgcttaaaa tctggcctta taggattatt tatca
//
LOCUS       DASWHZ010000141         4746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1017525, whole genome shotgun sequence.
ACCESSION   DASWHZ010000141 DASWHZ010000000
VERSION     DASWHZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017525"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGA49_02840"
     CDS             <1..501
                     /locus_tag="VGA49_02840"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX9664737.1"
                     /translation="RTAGNQLNNDIIQYIRNEFNVLLGEIAAEEVKIKIGSASILDDA
                     LEMKIRGRDLITGLPKEITVNDSQIRDALSRSVRLMTDDIKATLEVTPPELVADIYEK
                     GMVLTGGGALLKNIDKAIARVTNIPVQIADDPLTAVVRGTGIVLEDVDSVRDYILTSG
                     QEDNIK"
     gene            511..1317
                     /gene="mreC"
                     /locus_tag="VGA49_02845"
     CDS             511..1317
                     /gene="mreC"
                     /locus_tag="VGA49_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX9664738.1"
                     /translation="MKRLASPVRFSLIIAVIIILLIFFHYLAWLRPLENLIVRILSPV
                     QAQTLSVSNYFNSFFKKFSYDANLESENEQLRQELNELRLKHSQLQTALEENKIISRQ
                     VEFVESNLPDAKYLTARVIGRDQQNQRVFYINRGEKDGVASAAAVIIEAGFLIGKTLD
                     VFGDYSSFLLLTDNQSQTAAVIQGQSEINKLVTGEHGLSLKIDLIPQYEEVNLRDIII
                     SSGLENYIPKGLVIGQVEEVIENPNELFKSAIVKPLVAPDNISLVTVIIP"
     gene            1320..1838
                     /locus_tag="VGA49_02850"
     CDS             1320..1838
                     /locus_tag="VGA49_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664739.1"
                     /translation="MVRRFLNIIILVLLIIFQVSFINNLVFPFSQLNLILSFLIFLIM
                     TADIYLTLKIALALGFLLEFYSAVNFGFILISIFLTLILLYLLFINFFTNKSFYSLMI
                     LGLIGSVAYYFIFILINKLFYFFNLNFFEYSFEPVSLIWSAGLNLVILTIFFFGYNFL
                     THELKSYFIYGK"
     gene            1828..3807
                     /gene="mrdA"
                     /locus_tag="VGA49_02855"
     CDS             1828..3807
                     /gene="mrdA"
                     /locus_tag="VGA49_02855"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX9664740.1"
                     /translation="MGNDFFKHFNKNQIKDPDLRSRRDLNWTEDVYLSVDQKAGRLKP
                     GDKNEFLGLSVNFFRLKLFFGLLIVLLAVLLLRAVYLQIIQGDYYQLIAETNRIRLVP
                     IKAPRGVIYDRHQRQLVQNNPNFALYLIPIDFPDEHDQQTKILTEIYQLIQEKMDLSL
                     EEFLISFQEKLEGLNKYSYQPILIHDNLEHNDAVLLKIKSGQLPGIVLDLESKRNYLF
                     NNTISLSHLVGYLGRLNPDDYQSLKDKEYQYNDKIGVTGLEAVYEGFLRGLDGKKQVE
                     VNALGKTIKTVAQEAAVEGASLVLAIDFELQSYAEETLKKYLELNDKSRAAIIIQDPS
                     TGQVMTMVSYPPFDNNLFAGGISSQDYQALIGNSDQPLFNRAALGTYPSGSTFKPVVA
                     LAALEEAIISPSTTVLSQGGLRISSWFFPDWQAGGHGLTNVTKALAESVNTFFYMIGG
                     GYGDFEGLGVNRITDYAQKFGFGDLTGIDLNHEKAGFLPSAEWKETVKQEQWYIGDTY
                     HYAIGQGDVLVTPLQINNMMTVFANQGRLYQPSVAYGFIRNGDFKPVEPVILNQSFIS
                     PESLQTVRSGLRQAVTYGTARGLNSLPVTAAGKTGTAQWSSLREPHSWFTGFAPYEDP
                     RLVITVLVEEGGEGTDIAVPVAREILNWYFSRVDN"
     gene            3860..4327
                     /gene="greA"
                     /locus_tag="VGA49_02860"
     CDS             3860..4327
                     /gene="greA"
                     /locus_tag="VGA49_02860"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9664741.1"
                     /translation="MTDQKHYLTKARFEELQGELEALITVKRKEIAERIAEAIEMGDL
                     SENAAYTEAKDEQAFLEGRILELTDIINNANIINGNHVSGRVEVGSKVVVDCDDLGKK
                     EYTIVGATEADPLQGFISNESPLGEAFLGRKENDEFEINIPKGVTRCKIIRIE"
     gene            4417..>4746
                     /locus_tag="VGA49_02865"
     CDS             4417..>4746
                     /locus_tag="VGA49_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX9664742.1"
                     /translation="MAQIRERKERLKRLELIKQQGIDPYPPAGRRSHQIKEILTGFKD
                     LESAQKNLFIVGRIKSVRKHGGSQFIDLEDGTARIQVYLKKDVIGDDQYEFLSLTDIG
                     DFIEVEGG"
BASE COUNT         1350 a          860 c         1111 g         1425 t
ORIGIN      
        1 aggaccgccg gcaatcagct taataatgac attattcagt atattagaaa tgaatttaat
       61 gttttgctgg gggagatagc ggccgaagag gtcaaaataa aaattggttc agcttcaatc
      121 ctggatgatg ctctggaaat gaaaattcgc ggccgtgatc tgattaccgg cctgcctaaa
      181 gaaattacag ttaatgactc ccagatccgc gacgccttgt cccggtcagt ccggttgatg
      241 actgacgata ttaaggccac actggaagtg acaccgccgg agctggtggc tgatatttat
      301 gagaagggga tggtgctgac cggcggtggc gccttgttaa aaaatattga caaagccatt
      361 gcccgggtaa ccaatattcc ggtccagatt gccgacgatc cgctgacagc cgttgtccgc
      421 ggaaccggca ttgtcctgga agatgttgat tcagtcaggg attatatttt aacttccggc
      481 caagaagaca atattaaata aatttaatca atgaaaagat tagcctcgcc agttagattt
      541 agtttaataa tagcagtaat aatcatactg ctaatttttt tccattatct ggcctggctg
      601 cggccgcttg aaaatttaat tgtcaggatt ttgtcgcctg tccaggctca aaccttgtct
      661 gtcagcaatt attttaacag tttttttaaa aaatttagtt atgacgcgaa tttagaatca
      721 gaaaacgaac agcttcgcca ggagttaaac gaactaagat taaaacatag tcagcttcaa
      781 accgcgcttg aggagaacaa aataatcagc cgtcaggttg aatttgttga aagtaatctg
      841 ccagacgcca aatatttgac tgcccgggtg atcggccggg atcagcaaaa tcagcgggtt
      901 ttttacatta atcggggaga gaaagacgga gtggcgtcgg cagcggccgt gattattgaa
      961 gccggttttt taattggcaa aacacttgat gtttttggtg attattcttc gtttttattg
     1021 ctgactgata accagagtca gacggcggca gttattcagg ggcagtctga aattaacaag
     1081 ctggtgactg gtgagcacgg tttgagcctg aaaattgatt taattcccca atacgaagag
     1141 gtcaatctcc gggatattat catcagctcc gggctggaaa attatattcc caagggtctg
     1201 gtgattggcc aggttgaaga agtgattgag aatcccaatg agctttttaa gtcagccatt
     1261 gtcaagccgc tggttgctcc ggacaacatc agcttagtaa ccgttattat tccttaaata
     1321 tggtccggag atttttaaat attattattt tggtactgtt gataattttt caggttagct
     1381 ttattaataa tttagttttt ccttttagtc agttaaatct tattttaagc tttttaattt
     1441 ttttaatcat gacagctgat atttatctga ctcttaaaat tgccctggcg cttggttttt
     1501 tgctggagtt ttattcagcg gtaaattttg gatttatttt aatttcaata tttttgaccc
     1561 tgattctgct ttatttgctt tttataaact tttttaccaa taaatctttt tattcactga
     1621 tgattttagg tctgatcgga tcagtcgctt attacttcat ttttatcctg atcaataaac
     1681 tattttattt ttttaatctc aatttttttg agtacagttt tgaaccagtt agtctgattt
     1741 ggtcagccgg acttaatctg gtaattttga cgattttttt ctttggctat aattttttga
     1801 cccatgagct gaaaagctat tttatttatg ggaaatgatt tttttaaaca ttttaataag
     1861 aatcaaatca aagatcctga tttgcggtca aggcgcgatc ttaactggac ggaagatgtc
     1921 tatttgtctg ttgatcagaa ggccggccgg ctgaagcccg gcgataagaa tgaattttta
     1981 ggcttatccg taaatttttt tcgcttaaag ctgttttttg gtctgctgat tgttttactg
     2041 gccgttcttt tgttaagagc ggtttatctt cagataatcc agggggatta ttatcagctg
     2101 attgctgaaa ctaaccggat caggctggtg ccgattaagg cgccccgggg agtgatttat
     2161 gaccgccatc agcggcagct tgttcaaaat aatccaaatt ttgctttata cctgatccca
     2221 attgattttc ctgacgaaca tgatcagcaa actaaaattt taacggaaat ttatcagctt
     2281 attcaggaaa aaatggattt atcgctggag gagtttttga taagttttca ggagaaatta
     2341 gagggcctga ataaatattc ttaccagcca attttaattc atgataatct tgagcacaat
     2401 gacgctgtcc tgctcaagat taaaagtggc cagctgccgg gaatagtttt agatttggaa
     2461 agcaaaagaa attatttatt caacaatact ataagcttat ctcatctggt cgggtatctg
     2521 ggccggctga atccggatga ttatcaaagc ctcaaagaca aagaatatca atacaacgat
     2581 aaaatcggcg tcaccgggct ggaagcggtt tatgaaggtt ttttaagggg cctggacggc
     2641 aaaaaacagg ttgaggttaa cgctttgggc aaaactatta aaacggtcgc ccaggaggcg
     2701 gcggttgagg gcgccagcct ggtgctggcg attgattttg agctgcaatc ttacgctgaa
     2761 gaaaccctga aaaaatattt ggagctaaat gacaaaagtc gggcggccat aattattcag
     2821 gacccgtcga ccggccaggt tatgacaatg gttagttacc cgccttttga taataattta
     2881 tttgccggtg ggatttccag tcaggactat caggctttaa tcggcaattc ggaccagccg
     2941 ctgtttaacc gggcggcgct gggaacatat ccctcgggtt caacttttaa gccggtagtg
     3001 gccctggccg ccctggagga ggcaataatc agccccagca ctacagttct tagccaggga
     3061 ggtttaagga tcagttcatg gtttttcccc gactggcagg ccggtggtca tggcctgact
     3121 aatgttacca aggctctggc tgaatcggtt aatacatttt tttatatgat aggcggcggc
     3181 tatggtgatt ttgaaggttt gggagtgaac cggattactg actacgctca aaaattcgga
     3241 tttggcgacc tgacaggtat tgatttaaat catgaaaaag ctggtttcct gccttcagcc
     3301 gaatggaaag agactgttaa acaggagcaa tggtatattg gcgatactta tcattacgct
     3361 attggccagg gggatgtttt agtgactcct ttgcaaatta ataatatgat gacagttttt
     3421 gccaatcagg ggaggcttta tcagccttca gtggcttatg ggtttatcag gaacggggat
     3481 tttaagccgg ttgaaccagt tatattgaat caaagtttta tcagtccgga aagtcttcag
     3541 actgtcagat ccggtttgcg ccaggcggtg acttatggca ctgcccgcgg gttaaacagc
     3601 ctgccggtaa cagccgccgg caagacaggc accgctcagt ggagcagtct ccgggaaccc
     3661 cactcctggt ttactggttt tgctccatat gaagatccca ggctggtgat tacagtttta
     3721 gtcgaagaag gcggtgaggg gactgacatt gccgtgccag tcgccagaga aattttgaac
     3781 tggtatttta gtcgtgtgga taattaaacc ttgacagata ttttaaatta tatattctta
     3841 agactatatt aacttactta tgactgacca aaaacattat ctgaccaaag ccaggtttga
     3901 agaactccag ggggaactgg aggctttaat tactgtcaaa cgaaaagaaa ttgccgaaag
     3961 aattgctgag gcgattgaaa tgggggactt aagcgaaaac gccgcttaca ctgaagccaa
     4021 ggacgagcag gcctttttgg aaggacgaat tttggaactg actgatatta taaataacgc
     4081 caatattatt aacggtaatc atgttagcgg ccgggttgag gttggctcca aagtagtcgt
     4141 tgactgcgac gatttaggaa aaaaagaata tacaattgtc ggagcgacgg aagctgatcc
     4201 gctgcagggc tttatatcca atgaatcgcc gctgggcgaa gcttttctgg gcagaaaaga
     4261 aaatgatgaa tttgagatta atattcccaa aggggtgacc cggtgtaaaa taattaggat
     4321 tgaataaaga tttaaaaata ttgactaatt taaaaccctc tgatcgttca gagggttttt
     4381 gacagaatgc ttaaaattaa ttaaaattgt gataatatgg ctcaaataag agaaagaaaa
     4441 gaacgcctta aaaggcttga attaattaaa caacagggga tagatcctta tccgcccgct
     4501 ggccggcgca gtcatcaaat aaaagagatt ttgacgggtt ttaaggacct ggaatcagct
     4561 caaaaaaact tatttatagt cggccggata aaatcagtca gaaaacacgg cggctcccaa
     4621 tttattgatc tggaagacgg gacagccagg attcaggtct atttaaaaaa agatgttatt
     4681 ggagatgacc agtatgaatt tttaagtttg actgatattg gagattttat tgaggtcgag
     4741 ggcggt
//
LOCUS       DASWHZ010000142         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2141206, whole genome shotgun sequence.
ACCESSION   DASWHZ010000142 DASWHZ010000000
VERSION     DASWHZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2141206"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGA49_02870"
     CDS             <1..385
                     /locus_tag="VGA49_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664743.1"
                     /translation="SGPEGNQDKIITVSRERYARARETVEDKIKRWSGMIVEQSDLKK
                     EFKKPEEQKPDKPWLDKKKNNKKKRSPVFFRTSAPVKSSKEKHQDKITPPPISQAQPV
                     PKEVAPPISLKEALERTKNFSKDKQ"
     gene            387..1097
                     /locus_tag="VGA49_02875"
     CDS             387..1097
                     /locus_tag="VGA49_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9664744.1"
                     /translation="MPNKKKKDQKKFKEKKVVYLSQPFDFKEDVTWRIFRIMSEFIDG
                     FQFLADLKKEVTFFGSARVSASNEYYKQARQLCRLLGKSGFTIITGGGPGIMEAANQG
                     AFETGAESVGLNIQLPFEQRINPFVKKGIGFHYFFTRKVMLSASAQAYIFFPGGFGTL
                     DEFFEMTMLIQTKKMETIPIILVGQEYWGPLLDWINNVVYGKFKNIDREDMKIYTLVD
                     NIQEAYEIIRKTKERKYF"
     gene            1107..1904
                     /locus_tag="VGA49_02880"
     CDS             1107..1904
                     /locus_tag="VGA49_02880"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX9664745.1"
                     /translation="MTIFQFGKSKYLLLVFVFLLSGCLNLSQVKIDNQENTWLEISPG
                     LEYRQEEVLDETDSLRDVIVIFRIDPEFFRFSVVQDVNNPKTVLDWQKETKAGLVVNG
                     GYFDRNNLSTGLLIINQEKFGIESYDGSNGYTGMFLVRNGQPQLRYLPENNYDPQEQI
                     EFGLQSFPTLILSGGRLGAVQPTDKRARRTVIAQDKEKNILLILTKGSSFSLYDLMNY
                     LPKLNLNLEVAFNLDGGPSTGISANVSGFNYNIESAPVPNVILIREK"
BASE COUNT          603 a          359 c          445 g          560 t
ORIGIN      
        1 cagcggcccg gaaggcaatc aggacaaaat cattactgtt tcacgggaaa gatatgctcg
       61 cgcccgggag accgttgagg ataagattaa acgctggagc ggtatgattg tcgaacagtc
      121 agatttaaaa aaagaattta aaaaaccaga agagcaaaag ccggataaac cgtggttaga
      181 caaaaagaaa aataataaaa agaaaaggag ccctgtcttt ttcaggactt ccgctcccgt
      241 taaatcttca aaggaaaaac accaagataa gattactccg ccgccaatta gccaggccca
      301 gccggtcccc aaagaagtcg ctcccccgat ttctctcaaa gaagctttag agcggactaa
      361 aaatttttct aaagataaac aataatatgc ccaataaaaa aaagaaggat caaaaaaaat
      421 ttaaagaaaa aaaagtagtt tatcttagtc agccctttga ttttaaggaa gatgttacct
      481 ggcggatttt tagaattatg tcagaattta ttgacggctt tcagtttttg gctgatttaa
      541 aaaaagaagt gacctttttt ggctcggccc gtgtttctgc cagtaatgaa tattataagc
      601 aggcccgtca gctttgccgg ctgctgggca aaagcggttt tacgattatt accggcggcg
      661 gcccgggaat tatggaggcg gccaatcagg gcgcttttga aaccggcgcc gagtcagtcg
      721 gcctgaatat ccagctgcct tttgaacagc ggattaatcc ttttgttaaa aagggcattg
      781 gttttcatta tttttttacc agaaaagtga tgttgtcggc ttcagcccag gcttatattt
      841 ttttcccggg tggtttcggc accctggatg aattttttga aatgacgatg ttgattcaga
      901 ccaaaaaaat ggagacgatc ccgatcatcc tggtcggtca ggaatattgg gggccgcttt
      961 tggactggat taataatgtc gtttacggta agtttaaaaa tattgaccgg gaagatatga
     1021 aaatttatac cctggttgat aatattcagg aagcttatga gattatcagg aagactaaag
     1081 agcgtaaata tttttaatcg gaccaaatga cgatttttca gtttggtaaa tctaaatatt
     1141 tattgctggt ctttgttttt ttattgagcg gctgtctaaa cttaagccag gtcaaaattg
     1201 ataatcagga aaacacctgg ttggagataa gtcccggcct ggagtacagg caggaagagg
     1261 ttttagatga aactgattct ttgagggatg taattgtaat ttttagaatt gatccggagt
     1321 tttttagatt ttcagttgtt caggacgtga ataatcctaa aacagttctg gactggcaaa
     1381 aagaaaccaa agccgggctg gtggttaatg gcggttattt tgaccgtaat aacctgtcaa
     1441 ccggactgtt aattatcaat caggaaaaat ttggcattga aagttatgat ggttccaatg
     1501 gttataccgg aatgttttta gttcggaacg gccagcctca gcttagatac ctgccggaaa
     1561 ataattatga cccgcaggag cagattgaat ttggcctcca aagctttccg actctgattt
     1621 tatccggcgg ccggctggga gccgtccagc cgactgataa gcgggcccgc cggacagtca
     1681 ttgcccagga taaagaaaaa aatattttgt tgattttaac aaaaggcagc agctttagtc
     1741 tttatgacct gatgaattat ttgccgaagc ttaatttaaa tctagaagtt gcttttaatc
     1801 ttgacggcgg gccgtcaacc gggatttcag ctaatgttag tggctttaat tataatattg
     1861 agtcagcccc ggttcccaat gtgattctta tcagagaaaa gtaaatattt acaagatcgt
     1921 catcaaatgc tattatgcca gctaacaata ccaaaatcct ttttaag
//
LOCUS       DASWHZ010000143         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_277418, whole genome shotgun sequence.
ACCESSION   DASWHZ010000143 DASWHZ010000000
VERSION     DASWHZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277418"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="mraW"
                     /locus_tag="VGA49_02885"
     CDS             complement(<1..358)
                     /gene="mraW"
                     /locus_tag="VGA49_02885"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX9664746.1"
                     /translation="MSKKIKIRHVPVLTSEVIQFLNPGENKNFIDCTLGTGGHAKEIL
                     KRTGPAGKLLGIDLDQAALDIAGDELQEYSGRIILAKGNFKNIKKLTDDQQFNKINGI
                     LLDLGISSLQLAEADRG"
     gene            complement(351..782)
                     /gene="mraZ"
                     /locus_tag="VGA49_02890"
     CDS             complement(351..782)
                     /gene="mraZ"
                     /locus_tag="VGA49_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX9664747.1"
                     /translation="MFIGEYLHSIDDKGRLAIPVKFRAALSKGAVVTKGLDDCLFLYN
                     LEEWQKLADKLASLPISKANTRAFSRLMLAGAMNVQLDKQGRVVLPEYLRKYSGLKKK
                     VIVTGLYNRLEIWDEQKWNKYKEGTEKSSADIAEALGELDV"
     gene            complement(1061..1507)
                     /locus_tag="VGA49_02895"
     CDS             complement(1061..1507)
                     /locus_tag="VGA49_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664748.1"
                     /translation="MTGLIWKLQSLFIKQMYLVINTTDQLFFGLINEKGELTAQFSFP
                     GRVKSDYFIKQLKDFLKKNEIETAKLNGLGVVNGPGSFTAVRTGVVFANSLAFALNIP
                     IVSLRSDQFKDIDQLITKIYKKISQTDSSGRLINPIYDQAPGITRG"
     gene            complement(1467..>1648)
                     /locus_tag="VGA49_02900"
     CDS             complement(1467..>1648)
                     /locus_tag="VGA49_02900"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9664749.1"
                     /translation="YDLPGVPNILSFLGPYTIHGTYWHNNFGQPMSHGCINMTIADSK
                     WLYDWADLETSVIIH"
BASE COUNT          481 a          376 c          287 g          504 t
ORIGIN      
        1 agcccctgtc agcttctgcc agctgcaaag atgaaatccc aaggtcaagt aaaataccgt
       61 taattttatt aaattgttgg tcatcggtta atttttttat gtttttaaaa ttccctttgg
      121 ccagaataat ccgtcctgaa tattcctgaa gttcatcccc ggcaatatcc agggccgcct
      181 gatccagatc aataccgagt aatttgccgg ccgggccggt tctttttaaa atttctttgg
      241 cgtggccacc tgtccccaga gtgcagtcaa taaaattttt gttttctcca ggatttaaaa
      301 actgaattac ttctgatgtt aaaaccggga catgtctgat tttgattttt ttagacatct
      361 agttcaccta aagcttcggc gatatcagcg cttgattttt cagtcccctc cttatatttg
      421 ttccatttct gttcatccca gatttccaga cggttataca aaccggtaac tatcactttc
      481 tttttcaggc cgctgtattt tctcaaatat tcaggcagga ctactctacc ctgcttatcc
      541 agctgaacat tcatggctcc ggccagcatt aagcgggaaa aagcccgggt gttggcttta
      601 ctgattggca gactggccag cttgtcagcc agtttttgcc actcttctaa attgtaaaga
      661 aacaaacaat catccagtcc cttggtgacc accgctcctt ttgacagggc ggcccgaaac
      721 ttaaccggaa tagccaaccg gcctttgtca tcaattgaat gtagatattc gcctataaac
      781 atacaaaatt taccagatat aaacttcaag gccggggaaa ccggccgtga agtttacgaa
      841 agcatggaaa agcatggaac ccagattaat agagcgatca tttaacaatt ccgacttatc
      901 cccacttttc accacttatc accactctac ctatatttta tgccacgatc atccataagt
      961 caacacaaat cacccctttc aattttttgg cttaaataaa acaaaaaaag agcttttaac
     1021 tcttctttta cttcaaatgt ggacaatttg tgtaaaaaag ttatcccctg gtaatgccgg
     1081 gcgcctgatc gtaaatagga ttgatcaacc gccctgatga atctgtctga ctaatttttt
     1141 tatatatttt ggtgattaac tgatcaatat ctttaaactg atcagaccgc aggctgacta
     1201 ttgggatatt cagagcaaaa gccaaagagt tggcaaaaac cactcctgtt ctgacagccg
     1261 taaaactacc cggaccgttg actactccca gaccatttaa tttggcagtt tctatttcgt
     1321 tcttttttaa aaaatctttc agctgcttaa taaaatagtc tgatttaacc ctgcctggaa
     1381 acgaaaattg agcggtcagt tccccttttt cattaatcag tccaaaaaat aattgatcag
     1441 ttgtattaat aaccaaatac atttgtttaa tgaataatga ctgaagtttc caaatcagcc
     1501 cagtcataaa gccatttgga atccgcaatc gtcatattaa tacagccatg gctcatcggc
     1561 tggccaaaat tattgtgcca atatgtgccg tgaattgtat agggacctaa aaacgacaga
     1621 atattaggaa cgccgggcag atcataat
//
LOCUS       DASWHZ010000144         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2509590, whole genome shotgun sequence.
ACCESSION   DASWHZ010000144 DASWHZ010000000
VERSION     DASWHZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2509590"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VGA49_02905"
     CDS             complement(<1..402)
                     /locus_tag="VGA49_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9664750.1"
                     /translation="MIITWLGHSAFKIESKDITLVTDPYDPKIGFKFPKITADIVTIS
                     HDHFDHAYAEGVAGEPFIIDSAGEYEIKEVFVMGLPSFHDKKEGADRGGNIIYKIELE
                     NITIAHLGDLGHVLTDEQTEKLQGVDILMIPV"
     gene            complement(631..1344)
                     /locus_tag="VGA49_02910"
     CDS             complement(631..1344)
                     /locus_tag="VGA49_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664751.1"
                     /translation="MRPHIHTLNLLREILENIPENFPTSRKSEMEKEYQTMLSDEKIS
                     LDNIEETIRKFGGEVWPFRKAFEKMFELYGGRDLQQTVMDQLDPALKNKYQQYLNYHS
                     ERQKRIKEAVAEVTEHHKPASQIPDFLSNQEYLNIEQAEIDARKNITAKINQLVSGDK
                     ADEFQKYLQEFQNKQKLFSEKITGLRSLAGKYPGLAAEINDSVKTFQEAFSLITTDVD
                     LVEINKKIEDYRGRAGESI"
     gene            1538..2731
                     /locus_tag="VGA49_02915"
     CDS             1538..2731
                     /locus_tag="VGA49_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9664752.1"
                     /translation="MTTAIKTAENSQPDQLQEMERLIAEKKLRQIPKVGEIIDGKVIS
                     VSKSEVHLDLDGLTTGVVRGRELYDESGDYSNLKVGDQASATVLDLENEKGEMELSFM
                     HAGHRKAWDHLEELRATGAVIPAKIISANKGGLMARVGKVAGFLPVSQLSVDNYPRVE
                     GGDRGRILEKLNKLINQTMDVKVIDIDEKEEKLIVSEKAAWEEAQKETIAKYNVGDVV
                     DGKVTGVVDFGVFVEFGPGLEGLVHISELAWQRIDSPADIVKVGDQIKAQIISIDGAK
                     ISLSMKKLQEDPWKKEIEKFKVGDQIKGKILKVNPFGLFVELTENIHGLAHISELSDE
                     PIKNPRDHFKTGDQLDFKIISIEPDNHRLGLSLRERQEAQGTRHKEEERQPTEDTPET
                     AEEKK"
     gene            2825..4669
                     /gene="ftsH"
                     /locus_tag="VGA49_02920"
     CDS             2825..4669
                     /gene="ftsH"
                     /locus_tag="VGA49_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX9664753.1"
                     /translation="MKNLSKNIFIFLTVFLLILFVFSFINLSATKVEKIDIGTLINQI
                     ENEQVREISVEKDKINVVLKDDTQESVFKEKTESLSTLFSNYQVDPEKLKKVKIEIKE
                     ETGWDLFLTTVLPFLIPFLLIAVVIYFLMRQVQGANTRAMMFGQSSARQVSRDQKDRI
                     TFKDVAGVQEAKEELLEVVEFLISPKKFIQVGAKIPKGVLLMGAPGTGKTLLAKAVAG
                     EAKVPFFSISGSEFVEMFVGVGASRVRDLFKKAKKHAPCIVFIDEIDAVGRQRGAGLG
                     GSHDEREQTLNQILVEMDGFDPNAGVIVIAATNRPDVLDPALLRPGRFDRRVVLDLPD
                     INDRTAILKVHSVKKPLAEDVSLKEVAERTPGFSGADLANLLNEAAILTARRNKKKIG
                     HKEVVESIEKVLLGPERKSHMLSDKEKKVTAYHEAGHALVAHKLPHTDPVRKVSIISR
                     GRAAGYTLKLPEKDKHLQAQSEFIDELAVLLAGHVTEKEIFGEVTTGASNDLKQATKL
                     ARSIIAQYGMSEALGPRTFGQKEELIFLGREIHEQKDYSEKIAEEIDREISRLIADAC
                     RTAKKVITDHREDLEAIVSELMEHETLDREEFEAIFKESGKQGKIKKE"
     gene            4793..>5346
                     /locus_tag="VGA49_02925"
     CDS             4793..>5346
                     /locus_tag="VGA49_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664754.1"
                     /translation="MLTYHQIFKKAWQIVWQNKFLWFFGFFAALIGNSGGYEMLLNNL
                     TKVFSQENVLLGVKNFFSLVGSPQYLMHFKEKLFSQPSSFVFLALLYLILGAVLLFIF
                     WVIINSVSSLIYGAAQADNNKKISLKSVWTAAKKYFWPVLVVNLLAMIVIWLLAVFFS
                     WPILGLTLAAPSAGKGLLLFLLYLV"
BASE COUNT         1511 a         1063 c         1277 g         1495 t
ORIGIN      
        1 gaccgggatc attaaaatat caacgccctg aagtttttct gtttgttcgt cagtcagaac
       61 atggcccagg tcacccaggt gggcgatggt gatattttcc agttcaattt tgtagatgat
      121 attgccgcct ctgtcagcgc cttctttttt gtcgtgaaaa gaagggaggc ccatcacaaa
      181 aacttcctta atttcatact caccggcgct gtcaataata aaaggttcgc cggcgacgcc
      241 ttcggcgtaa gcgtggtcaa agtggtcgtg actgatggtc acaatgtcag ccgttatttt
      301 gggaaattta aaaccgattt taggatcgta gggatcggtc actaaagtaa tgtctttgct
      361 ttctatttta aaggcggagt ggcccagcca ggtgatgatc atagttttgt gatttagagt
      421 tgttcgtaaa aattttggta atcgttatta gttattggtt ttagctgccg gtatggatag
      481 gggtttggtt ttaggtgtgg cagccggtat ggtctgtggt tttagtttta ggttttttta
      541 cctaaaccca caccctaaaa ctatactagc tgccacaccc aaaccctata actaaaaccg
      601 atacggatag cgataacgaa gaactaaaac ctatattgat tcaccggctc gtcctctata
      661 atcttcaatc ttcttattta tctccaccaa atcaacatca gtcgtgatta aactgaacgc
      721 ttcctgaaaa gttttgaccg aatcattaat ttcagccgcc aggccgggat attttccggc
      781 cagagaccgg agcccggtta ttttttcact gaaaagtttt tgtttgtttt gaaattcttg
      841 taaatatttt tgaaattcat cagccttatc gccgctaact aactgattaa tttttgcggt
      901 aatatttttt ctggcgtcaa tttcagcctg ttcaatgttc agatattctt gattgcttaa
      961 aaaatcagga atctgactgg cgggtttatg atgttcagtc acttccgcca cggcttcttt
     1021 aattcttttt tgtcgttcag agtgataatt tagatattgc tggtatttgt tttttaaagc
     1081 cggatcaagc tggtccatga ctgtttgttg aagatcgcgg ccgccgtaaa gttcaaacat
     1141 tttttcaaaa gctttccgaa aaggccagac ttcgccgccg aattttctga tagtttcttc
     1201 aatgttatcc aaagaaattt tttcatcaga aagcatggtc tgatactctt tttccatttc
     1261 agacttgcgg cttgttggga aattttcagg aatattttcc agaatttccc gaagtaaatt
     1321 gagggtatga atgtgtggac gcattaaaac attttaacat aaaaaggcaa tgaaataaag
     1381 tgtattacaa ttctcttgaa aaatattatg ctttgtatta taatagtaaa aatccctaat
     1441 tattaaggct ttttgccgaa aattagggaa aatccacaca tcagaaagga gaaaggttat
     1501 cttttttaaa aagcgctagc cttttttaat ttctcttatg acaacagcga tcaaaacagc
     1561 cgaaaacagc cagccagacc agctccagga aatggagcgg ctgattgctg aaaaaaaact
     1621 gcgccaaatt cctaaagtgg gcgagattat tgacggcaaa gtcatcagcg tttccaaaag
     1681 cgaagttcat cttgatctgg acggcctgac caccggggtt gtccggggcc gggagcttta
     1741 tgatgaatcc ggcgattact ccaatttaaa agttggcgat caggccagcg cgacggttct
     1801 tgatttggaa aatgaaaaag gcgaaatgga attgtcgttt atgcacgccg gccaccgcaa
     1861 agcctgggac cacctggaag aacttcgggc gaccggagcg gttatcccgg ctaaaattat
     1921 cagcgccaat aagggcggcc tgatggcccg ggtcggcaaa gtcgccggct ttctgccggt
     1981 ctcgcagttg tcagttgaca actatccgcg ggtggaaggc ggcgatcgcg gcagaatttt
     2041 ggaaaaatta aacaagctga ttaatcagac catggacgtt aaggtgattg acattgatga
     2101 aaaagaagaa aaattaattg tttcggaaaa agccgcctgg gaagaagccc agaaagaaac
     2161 catcgccaaa tataatgttg gcgacgtggt tgacggcaaa gtgaccggcg tggttgactt
     2221 cggagttttt gttgaatttg gtccggggct ggaaggcctg gttcacattt ccgaactggc
     2281 ctggcagcgg attgacagtc cggctgacat tgtcaaagtc ggcgaccaga tcaaagccca
     2341 gattatcagc attgatggcg ccaaaatttc tttgtcaatg aaaaagcttc aggaagatcc
     2401 ctggaaaaaa gaaattgaaa aattcaaagt cggcgaccag attaagggta agattttaaa
     2461 agttaatccg tttggcttgt ttgttgagct gactgaaaac atccacggtc tggctcacat
     2521 ttccgaactg tctgatgaac caattaaaaa tccaagagac cattttaaga ctggcgatca
     2581 gctggatttc aaaattattt caattgaacc ggataatcac cggctgggat tgtcgttgag
     2641 ggaaaggcag gaggcacaag gcacaaggca caaggaagag gaacggcaac cgacagaaga
     2701 taccccggaa actgctgaag aaaagaaata atcaagttaa atcttgtaaa atataaaaaa
     2761 gcctgcttca ggctttttat ggttgaaata atgtcaaaaa tgttataata taaaattaat
     2821 cattatgaaa aatctctcca aaaatatatt tattttttta acggtttttt tactgattct
     2881 gtttgttttc agcttcatta atttatcagc caccaaggtt gaaaaaattg atataggaac
     2941 tttgatcaat cagattgaaa acgagcaggt cagggaaatt tcagttgaaa aagataaaat
     3001 caatgtcgtc ctcaaagacg acacgcagga aagcgttttt aaagaaaaaa ctgaatcatt
     3061 gagcactctt ttcagcaatt atcaggttga tccggaaaaa ttgaagaagg ttaaaattga
     3121 aattaaggaa gaaaccggct gggatttgtt tttgacgact gttttgccgt ttttgattcc
     3181 ttttctgctg attgccgttg ttatttattt cttaatgcgc caggttcagg gagccaatac
     3241 ccgggcgatg atgtttggcc agagcagcgc ccgccaggtc agtcgcgatc aaaaagacag
     3301 gattactttt aaagacgtgg ccggcgttca ggaggccaaa gaggaactgc tggaagtggt
     3361 ggaatttttg atcagcccca aaaaatttat tcaggtcggc gctaaaattc ccaaaggcgt
     3421 tttgctgatg ggcgcgccgg gaaccggtaa aactttgctg gccaaagcgg tcgccggcga
     3481 agccaaagtg ccctttttca gtatttccgg atcagagttc gtggaaatgt ttgttggcgt
     3541 tggtgcttcc cgggtcagag atttatttaa aaaagccaaa aagcacgctc cctgcattgt
     3601 ttttattgat gagattgacg ccgtcggccg ccagcggggc gctgggttgg gcggatcgca
     3661 tgacgagcgg gagcagactt taaatcaaat tttggtggaa atggacggct ttgaccccaa
     3721 cgccggggtg attgtgattg ccgccaccaa ccggccggat gttctggacc cggctttgct
     3781 aagaccgggc cggtttgacc ggcgggtggt gctggatttg ccggacatta atgaccggac
     3841 ggctatcctt aaagtccaca gcgttaaaaa acctttggct gaagatgttt ctctgaaaga
     3901 ggttgccgaa agaacccccg gtttttccgg agccgatctg gctaacttat taaatgaagc
     3961 cgccatcctg accgcccgcc ggaataaaaa gaaaatcggt cataaggaag tggttgaaag
     4021 cattgagaaa gttttgctgg gtccggagag aaagagccac atgctgtcag acaaagaaaa
     4081 gaaagtgacc gcctaccacg aggccggcca cgctttggtg gcccacaaac tgccccacac
     4141 cgacccggtc agaaaagttt ccattatttc ccggggccgg gcggccggct atactttgaa
     4201 attaccggaa aaagacaaac atctccaggc gcaatcagag tttattgacg aattggcggt
     4261 tttgctggcc gggcacgtta cagaaaaaga aatttttggc gaagtgacca ccggcgcttc
     4321 caatgattta aagcaggcca ctaagctggc ccggagcatt attgcccagt acggcatgag
     4381 cgaggcgctg gggccgagaa cttttggcca aaaagaagaa ttgattttcc ttggccggga
     4441 aatccatgag caaaaagatt acagtgaaaa aatcgccgaa gaaattgacc gggaaatcag
     4501 ccggctgatt gccgacgcct gcaggacagc caaaaaggtt attactgacc atcgggaaga
     4561 cctggaagcc atagtctccg aactgatgga gcatgaaact ctggaccggg aagaatttga
     4621 ggcgatcttt aaggaaagcg ggaaacaggg taagattaag aaagaataat aagtcataaa
     4681 attattaatt taaaaaatga ctacttagag gcattaggct ctggtcatta gtcattaggc
     4741 agtaggcatt gattagtaat taggtcaatt agaacttaga aatttttaga ttatgttaac
     4801 ctaccatcaa attttcaaaa aagcctggca gattgtctgg caaaacaaat tcctgtggtt
     4861 tttcggtttt ttcgcggctt tgatcggcaa cagcggcggc tatgaaatgc ttttgaataa
     4921 tttgacaaaa gtattttctc aggaaaatgt tctgctgggc gttaaaaatt ttttcagtct
     4981 ggtcggctcg cctcaatatc tgatgcattt taaagaaaaa cttttcagcc agccctcgtc
     5041 ttttgttttt ttggctttgc tgtacctgat tttaggggcg gttcttttat ttattttctg
     5101 ggtgattatt aattcagtca gcagcctgat ctacggcgcg gcccaggctg acaataataa
     5161 aaaaatttct cttaagtccg tctggacagc cgccaaaaaa tatttctggc cagttttggt
     5221 cgttaatttg ctggcgatga ttgtcatctg gctgctggcc gtatttttta gctggccgat
     5281 tcttggtttg acgctggcgg ccccgtcagc cggtaaaggc ctgttgttgt ttttgctgta
     5341 cctggt
//
LOCUS       DASWHZ010000145         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_279606, whole genome shotgun sequence.
ACCESSION   DASWHZ010000145 DASWHZ010000000
VERSION     DASWHZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279606"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VGA49_02930"
     CDS             complement(<1..1119)
                     /locus_tag="VGA49_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664755.1"
                     /translation="MSNSMKKRIELSPEEVRAMMAEVKRMKKGEAPSAVEIPEGREKG
                     ERPAEQKGIFGGALDWLKRGRQEAETVAQDNKEVLNVSGSGGSIPVADLTANFEQTAA
                     DFGSDLAEEEEELVEAAENSLEKILGYDQDAEIKKLNQEIARIKKEIKNLDKAKDDEV
                     AEFYKEHDRPDTFVQPEGLKFEKGHGQILPPAYTDKDVQAEFEKFQKEQKKYDKSNLD
                     LVKKKSELENKKRRRIDQLQFAEKLAAKELIPQKQIADEIVRLEKRKQSLESGKMTII
                     PEFGSQEEQIKEIETEIQSKEKELSDLEEGEKKVDAIVNKFYSKMTYSEILKDEIAYL
                     EEIAAEYDKYPDTVIDNFGLRQERDRLNIEMNIKKIELE"
     gene            complement(1319..1828)
                     /locus_tag="VGA49_02935"
     CDS             complement(1319..1828)
                     /locus_tag="VGA49_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9664756.1"
                     /translation="MIKHPDQRVGVFVDVSNMYHSAKNLYNARVNFKEILKEAVDGRK
                     LIRAIAYVIESKSREEQSFFGALDKLGFEVNMKELQIFTTGMKKADWDVGLAVDTIKL
                     AGKLDSIILVTGDGDFIPLVYFLQETKGCMVEVIAFKETTSSRLIEAADDFLDISSNK
                     RKFLIKAKS"
     gene            complement(2027..2296)
                     /gene="rpsO"
                     /locus_tag="VGA49_02940"
     CDS             complement(2027..2296)
                     /gene="rpsO"
                     /locus_tag="VGA49_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9664757.1"
                     /translation="MLTKEQKDKIIKKFKIHETDTGSSEIQIAILTEEIDMLTGHLKQ
                     HKKDFSSRRGLIKKVADRRSLLNYLKRSDPPAFEALIKKIKLKVK"
     gene            2656..>3386
                     /locus_tag="VGA49_02945"
     CDS             2656..>3386
                     /locus_tag="VGA49_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9664758.1"
                     /translation="MTLNQHIKQFLEYLEIEKGRSQRTCRNYEFYLKRFADWAKDPSP
                     DKITTPVIKNYRLWLNRLTDAKNQSLKKNTQNYHLIALRSFLKYLAKNDVKTLASEKI
                     ELAKTPERTVEFLEGSDLERLLEAPQRIDNPDIIKKRDKAILELLFSTGLRVSELTSL
                     TKDSLNLQKDEFTIRGKGDKPRIVFLSNQSKFWIRQYLEARKDMDPALFVSHDRAKKG
                     RPNEKTANPLTPRSIQRLVKKYSKAA"
BASE COUNT          913 a          793 c          619 g         1061 t
ORIGIN      
        1 ttctaattca atttttttta tattcatttc aatgttcaat ctatcccgtt cctgcctcaa
       61 cccaaaatta tcgataactg tatcgggata tttatcatat tcagcggcaa tttcttccaa
      121 ataagctatc tcatccttta aaatttcaga ataagtcatt tttgaataaa atttattgac
      181 aatggcatca actttttttt cgccctcttc cagatcagac aattcttttt ctttagactg
      241 aatttcagtt tcaatctctt taatttgctc ttcctgtgaa ccaaactccg gaatgatcgt
      301 cattttgcct gattccaaac tttgttttcg tttttccagc cggacaatct catcagcgat
      361 ctgcttttgc ggaatcagct ctttggcagc taatttttca gcaaattgaa gctgatcaat
      421 tctccgtctc tttttatttt ccaactcgct tttctttttt actaaatcca aatttgattt
      481 atcatatttt ttctgttctt tctgaaattt ttcaaattct gcctggacat ctttatcagt
      541 ataagccggc ggcagaatct ggccatggcc tttttcaaat tttaaacctt cgggctgaac
      601 aaatgtatcc ggcctatcgt gctctttata aaattcagca acttcatcat ctttggcctt
      661 gtctaaattt tttatctctt ttttaattct ggcaatttcc tgattcagct ttttaatttc
      721 cgcgtcctga tcatacccca gtattttttc caaactattt tcagccgctt caacaagctc
      781 ttcctcctct tcggccaaat cactcccaaa atcagccgcc gtctgttcaa aattagctgt
      841 caaatcggct accggaattg agccccctga acccgaaaca tttaaaactt ctttgttgtc
      901 ttgggcgact gtttcagctt cttgccgacc tcgtttcaac cagtccaaag ccccgccaaa
      961 gattcctttt tgttcagccg gccgctcacc cttttccctg ccctccggaa tttcaacggc
     1021 ggagggagct tctccttttt tcatccgttt cacttcagcc atcatcgccc gaacttcttc
     1081 cggcgacagc tcaattcttt ttttcatgct gtttgacata tagtataagg cactaggcac
     1141 taggcaatgg gcacaagggt tagtgtcccc gccttgggcg ggggacaatt tttttgatgg
     1201 gcatcaggca aagggtgtgt taaaaactat tgcctaaccc cctttgccta gcctattgcc
     1261 ttgtgccttg tgcctcgtcc cgccgctcgc gagcggcggg acctcgtgcc ttgtcccttt
     1321 aacttttggc ttttattaaa aactttcttt tgttggagct aatgtccaaa aaatcatccg
     1381 ccgcttcaat cagccgggac gaagtggttt ctttaaaagc gatcacctcc accatgcagc
     1441 ccttggtttc ctggaggaaa tagaccaaag gaataaaatc accgtcgccg gttactaaaa
     1501 ttatgctgtc cagcttgccg gccagtttga tagtgtcaac cgccaggcca acgtcccagt
     1561 cggctttttt catgccggtc gtgaaaattt ggagctcttt catgttgact tcaaacccca
     1621 gcttatccag agctccgaaa aaagactgct cctcccggga ttttgattca atgacatagg
     1681 cgatcgcccg gatcagcttc cggccgtcaa ccgcttcttt taaaatttct ttaaaattca
     1741 cccgggcgtt gtataagttc ttggccgagt ggtacatatt ggaaacatcg acaaatacgc
     1801 cgactctttg atctggatgt ttaatcatat aattacgaat taggcaatag gcaatgggca
     1861 ataggcacaa gggttggtgt ccccgccttg ggcgggggac agttttttga tagggttaag
     1921 gcaacagtga atgctattgc ctaaaccctg ttgactcgcc ttgtgcctcg tgccttttgc
     1981 cttttgcctg taaaaaaagc agcttccgct gcttttttta ctttttttac ttaactttta
     2041 attttatttt cttaattaag gcttcaaaag ccggcggatc agaccgcttc aaataattca
     2101 gcagactccg gcggtcagcc acttttttaa tcagcccccg gcgtgaagaa aaatcttttt
     2161 tatgctgttt aaggtgcccg gtcaacatat caatttcctc tgtcaggatg gcgatttgga
     2221 tttcagaaga accagtgtct gtttcgtgaa tcttgaactt tttaatgatt ttatctttct
     2281 gctctttggt taacataaag acctctgcag acgaaatcca cagccggcgt ccaaaattct
     2341 atggattgga ctcaacgcca gccccagaaa ccgtcagtta attatagata gatttaatta
     2401 aatctatcat atatgataaa aatttgcaag gatagaaata gtgtcgtaaa attaatatac
     2461 gcgatgacct gtcagtcgcg actgacgggc cgatgccaat atactaatgg atactaatga
     2521 aaccaatttt gtgatactag tatactaatt catactaatt tattagtttc attagtatca
     2581 attcgtatat tagaatcgag atataagtat aattagtatc cataagtata ttagcatcgt
     2641 atatatcgta tatttatgac acttaatcaa cacatcaaac aattccttga atatctggaa
     2701 attgaaaaag ggcggagtca gcggacctgc cggaattatg aattctattt aaaaaggttt
     2761 gccgactggg ccaaagaccc ctctcccgat aaaatcacca ctcctgttat caaaaactac
     2821 cggctgtggc tcaaccgatt aacggacgcc aaaaatcaat ccttaaaaaa gaacacccaa
     2881 aattaccatt tgatcgcttt aagaagcttt ttaaaatatc tggcaaaaaa tgacgtcaaa
     2941 actttggcgt cggaaaaaat tgagctggcc aaaaccccgg aaagaacagt tgaattttta
     3001 gaaggctctg atctggaaag actgctggaa gcgccccagc ggattgataa tccagatatc
     3061 atcaaaaaaa gagacaaagc gatcctggaa ctgctgtttt caaccggtct ccgggtgtct
     3121 gaactgactt ccctgaccaa agattcactt aatctgcaga aagacgaatt tacaattagg
     3181 ggtaaaggcg ataaaccaag gattgttttt ttatccaacc agtccaaatt ctggatcagg
     3241 caatatctgg aagccaggaa ggatatggat ccggctctgt ttgtcagcca cgaccgggct
     3301 aaaaaagggc ggcctaatga aaaaacagcc aatccgctga ctccccgtag tatccaacgt
     3361 ttagtcaaaa aatattccaa agcggc
//
LOCUS       DASWHZ010000146         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3244022, whole genome shotgun sequence.
ACCESSION   DASWHZ010000146 DASWHZ010000000
VERSION     DASWHZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244022"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..763
                     /locus_tag="VGA49_02950"
     CDS             224..763
                     /locus_tag="VGA49_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9664759.1"
                     /translation="MKDLRDKYLVYKYQKTNSPEVFGAIYDQYIDKIYRFIYFKVRTR
                     QEAEDLTSEVFLKLWQKITNKAEVDNLNAYIYAIARNSVIDYYRQKSTDKEVALEELA
                     ELGGDAETIGKITQASDVSQLMKAINSLKDEYREVILLRYIEELAIKEIALILNKSQG
                     ATRVLLHRALKTLREVISS"
     gene            769..1935
                     /locus_tag="VGA49_02955"
     CDS             769..1935
                     /locus_tag="VGA49_02955"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HEX9664760.1"
                     /translation="MSHNLRKNLKKLRQIRPDYNWKVSNRAFLLNEILKETPINRVVN
                     NQKFKFSDLVKLLTRDFSLKLVTRPVLASFLVAGLVLGGGITSVGASWDSLPGDTLYS
                     VKRTYEKAQVVLTIDKYKKAELGVNLVKKRVDELRQIKQNDQSKSKKIEKINLAAGEI
                     RREVKITKDNLNKIKSDKEIARVVRVAKVIDVEVNKIKKTLKEQKENIDQEIIEKTKE
                     KIDQALDAIKEAGVEAAVIIVEKHVSGEAGISEEEVVELINTKIESTKQELESAKEKV
                     EVLAETAKNVEIFAEEPVEKSDDDETNSAESAEKADQFEETPELKGEAGEFDEAEKSL
                     TEVENLMENKDFGTALSKITEVEKLIEEAGEKSEKVPTAEEASPSESDDQLPTE"
     gene            2220..>3659
                     /locus_tag="VGA49_02960"
     CDS             2220..>3659
                     /locus_tag="VGA49_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664761.1"
                     /translation="MKINQMDFKEKKIYMSNFIKKAFTVGVVMTTIVWSLGLAAFPLT
                     AGAQTTIAAGDLITAPGTQAVYYYDGTNRWVFTNDKNYWTWYKDFLSVKEITDLELGD
                     IPIGGNVVYQAGRRMVKITTDPKTYLVGENGMLHHVTSEQLAMDMFGASWNQAIDDVS
                     DAFFTNYQIGAAITDLSNLPVGAYFMSGGNRYYVDDSGVLRLDFPVTAFNVAPNFIGA
                     EFMAALAGSADLPDTSGATPVANYTDFLGMVPNASGTAGVPTPGPANVTATLSLPSGT
                     QTVLTNQGSTWLGTVNFNNAGSATTLDSYHVSLGGALGFNSMGNVWGVFGGEPTNNPL
                     NFASSTRDRQVFVNKALPAGSSSLQIYASGTVNGGGSTGSFDINNVMVAGGTLELNGM
                     VPINRSITAANTLTLANGATPPTGNIGGTNQEVFNFTANFGRAGTLDRIFVYESGTGD
                     VNKLDNCQLTAQTRTINGNVVGSYVDFNFG"
BASE COUNT         1143 a          679 c          823 g         1014 t
ORIGIN      
        1 taatggctcg tcggatagtt tgctggttga cgccaaacag tctggccgct tctgaaactg
       61 ataaacgaat tagagtaata ttatttgttg gattaaccat gtttgtatag gctatattat
      121 acaaataaca taaatttgtc aaattatgct tgaaaaatat aaatatttct gatataattt
      181 ttataaactt tgtaacagtt ttattttttt ttcgtctaat ttaatgaaag accttcggga
      241 taaatactta gtatacaaat accaaaagac taattcccca gaagtttttg gagcgattta
      301 cgatcagtac attgataaaa tttaccggtt tatttatttt aaggtcagaa ccaggcagga
      361 agctgaagat ctgacatcag aagtcttttt gaagctttgg caaaagatta ctaataaggc
      421 cgaggttgat aatttaaacg cttatattta cgctatcgcc cgtaattcag tgattgatta
      481 ttatcgtcag aagtcaactg ataaagaggt cgctctggaa gaattagctg aactgggtgg
      541 agacgcggaa acgattggca aaataaccca ggcttcagat gttagtcagc taatgaaagc
      601 tatcaatagt ctcaaggatg aatatcgcga ggtaatttta ttgagatata ttgaagagct
      661 ggctatcaaa gaaattgctt taattttgaa taaatcacag ggagcaaccc gggttttgct
      721 tcaccgggcc cttaaaacat tgcgggaagt tataagttca taatcaccat gtctcataat
      781 ttaagaaaaa atttaaaaaa acttcgtcaa atcaggccgg attacaactg gaaagttagt
      841 aatcgggctt ttttgctgaa tgaaatttta aaagaaacgc ctattaaccg ggttgttaat
      901 aatcagaagt ttaaatttag cgacttagtc aaattattaa cgcgtgattt ttctttaaaa
      961 ctggttacca ggccggtttt agcttctttt ttggtggctg gtttggtttt gggcggcggc
     1021 ataacgtcag tcggcgcttc ctgggatagc ctgcctggcg acactttgta ttcagttaag
     1081 agaacatatg aaaaggcgca ggttgtttta actattgata aatataaaaa ggctgaatta
     1141 ggtgtaaacc tggttaaaaa aagggttgat gaactgcgac aaattaaaca aaacgatcag
     1201 tcaaaatcca aaaaaattga aaaaattaat ttagcggccg gtgaaattag acgagaagtt
     1261 aaaataacca aagataattt aaataaaatc aagtccgata aagaaatagc cagggttgtg
     1321 cgggtcgcca aagtgattga tgttgaagtc aataaaataa agaaaacttt aaaagagcaa
     1381 aaagaaaata ttgatcagga aattatagaa aaaaccaaag aaaaaataga tcaggccctt
     1441 gacgcgatta aggaagccgg ggttgaagcc gccgtcatta ttgtcgaaaa gcatgtcagc
     1501 ggagaagccg gtatttcgga agaagaggtc gtggaattga ttaacaccaa aattgaatca
     1561 accaaacaag agctggaaag cgccaaagaa aaagttgagg tgttggcgga aacggctaaa
     1621 aatgttgaga ttttcgcgga agagcctgtt gaaaaatctg atgatgatga aacaaattcg
     1681 gctgaatcag ctgaaaaagc agatcagttt gaagagaccc cagagctgaa aggagaggcg
     1741 ggtgaatttg acgaggctga aaaaagcctg acagaagttg aaaatctgat ggaaaataag
     1801 gattttggca cagctttgtc taaaattacc gaagttgaaa agttgattga agaagccggt
     1861 gaaaaatcag aaaaagttcc gaccgctgaa gaagcatctc cgtcagaatc tgatgatcaa
     1921 ctacccactg aataaatgtt atccacagga ttgacattaa gccagaataa gttagaatta
     1981 accttgacaa tataaataaa acttgttgaa aaataactgc tctttgtagt taaattattt
     2041 tattaattgg attaattgga gtttagaagc gcataacagc gcctaaacta aataattaaa
     2101 agttcgtttg taattaattt tcgctaaaat tagggatttt gactcacttg ttggttaggc
     2161 tcaaggtcga atccgatacg gatgtctaat caagccggtg aattcagtcg aaatctctga
     2221 tgaaaattaa ccagatggac tttaaggaga aaaaaattta tatgagtaat tttattaaga
     2281 aagcctttac agtcggcgtg gtaatgacga cgattgtctg gtctttgggc ttagcggctt
     2341 ttccgttgac tgccggcgcg cagacaacaa tcgccgctgg tgatttaatc accgctccgg
     2401 gcacccaggc agtttactac tacgacggca ccaatcgttg ggttttcacc aatgataaaa
     2461 attattggac ctggtacaaa gactttttaa gcgtcaagga aatcactgat cttgaattag
     2521 gtgatattcc aattggcggc aacgttgttt atcaggccgg ccgacgaatg gtcaagatca
     2581 cgaccgatcc aaagacctat ttggttggcg aaaatggcat gcttcatcac gtcacttccg
     2641 aacagctggc catggatatg tttggagcca gctggaatca ggcgattgac gatgtttccg
     2701 acgccttttt caccaattac caaatcggcg cggctattac tgatttaagc aacctgccgg
     2761 ttggcgctta ctttatgtca ggcggcaatc gctactatgt tgatgattcc ggtgttttaa
     2821 ggctggattt tccggtgacc gcttttaatg tcgctcctaa ctttattgga gctgaattta
     2881 tggccgcttt ggctggttca gccgatttgc ctgataccag tggcgccact ccggtagcca
     2941 actacacgga ttttctggga atggtcccaa atgcatctgg cacagccggc gttccaactc
     3001 ctggtccggc taatgtcacc gcgactttaa gcctgccttc aggcacccag acggttctga
     3061 ccaaccaggg ttcaacctgg ctgggaacag ttaactttaa caatgccggt tcagccacga
     3121 ccctggattc ataccatgtt tcactgggcg gcgctttagg cttcaactca atgggtaatg
     3181 tttggggtgt ttttggcggc gagccaacca acaacccgct gaattttgcc tcatcaacca
     3241 gagaccgaca agtctttgtt aacaaagctc tgccagcagg cagttccagt ctgcaaattt
     3301 acgccagcgg cacggttaat ggcggcggtt caaccggtag ttttgacatt aacaatgtca
     3361 tggtcgccgg cggcactttg gaactgaacg gcatggtgcc aatcaaccgc agcattaccg
     3421 cggctaatac tttaacctta gccaacggag ccacgcctcc aaccggtaat attggcggta
     3481 ccaatcagga agttttcaac tttaccgcta actttggccg ggctggcact ttagacagaa
     3541 tatttgttta cgagtcaggg actggtgacg tcaataaact ggataattgt caattgacgg
     3601 cccagaccag aacaattaac ggtaatgtcg ttggttctta cgttgacttt aattttggc
//
LOCUS       DASWHZ010000147         7086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2880451, whole genome shotgun sequence.
ACCESSION   DASWHZ010000147 DASWHZ010000000
VERSION     DASWHZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880451"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VGA49_02965"
     CDS             <1..769
                     /locus_tag="VGA49_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HEX9664762.1"
                     /translation="EPRFIVMEYDGGDSKEKPLVLIGKGITFDTGGLNLKPSDGIYQM
                     HLDMSGGAAVLYSVLLAARLKLKKNIVGLIPAAENMISGSSYRPGDILKTMSGKTVEV
                     LNTDAEGRLVLADALTYAGKFYKPRLVISVATLTGAALVALGQYASAIFSKDESLLKL
                     LRNLGEKSGDYVWPLPLWEEFEKEMKGTFADLNNLGKSKYGGASQGAAFLAHFAKKYP
                     WLHIDIAPRMTAGEGEYLAKGAVGAPVRLLIRFLETY"
     gene            785..1468
                     /gene="pyrH"
                     /locus_tag="VGA49_02970"
     CDS             785..1468
                     /gene="pyrH"
                     /locus_tag="VGA49_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9664763.1"
                     /translation="MKKETIIISLGGSIIIPDKLDIVFLQNFKKLILKHIRQSRRFFI
                     VTGGGQTARIYQKGAKKIDQVSADDLDWLGIHASRLNAHLLRTIFKKQAHPVVIKEPD
                     VYKRIKEDIIIGAGWQPGWSTDYVCAYLARTYKIKNIVNLSNIAYVYDKDPNKFKQAQ
                     KVKKISWPDYRKIVGNKWVPGANFPFDPVAAKLSQKLGLKVSILKGGDLKNFDDYLMG
                     HKFKGTVIN"
     misc_feature    1503..1628
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1634..2173
                     /gene="rplJ"
                     /locus_tag="VGA49_02975"
     CDS             1634..2173
                     /gene="rplJ"
                     /locus_tag="VGA49_02975"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9664764.1"
                     /translation="MPVNKKQKEEIVSDLVDKLKKSKSVIFVNFDQVTVSEIEEFRQL
                     CREQAIDYSVSKKTLLKIAMDKGNLKEVDPLAFENGVGTLFSYDDEVAPAKIVKNFSK
                     LHENLKMIGGILASNPEGQRYLALAEVENLSMIPSKDELLARVVGSLNAPVAGLVGVL
                     SGNLRQLVYVLNAIGESKQ"
     gene            2352..2738
                     /gene="rplL"
                     /locus_tag="VGA49_02980"
     CDS             2352..2738
                     /gene="rplL"
                     /locus_tag="VGA49_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX9664765.1"
                     /translation="MEVPDKFKALVEQIEQLSVLDLAELVKVLEEKFGVSAAAPVAMA
                     AMPGAGGEAVPAEEKSEFSVELTEAGSNKIAVIKAIRGVIEMGLKEAKDLTEGAPQII
                     KEGVKKEEAEEMKKKLEEAGAKVTLK"
     gene            complement(2790..5138)
                     /locus_tag="VGA49_02985"
     CDS             complement(2790..5138)
                     /locus_tag="VGA49_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664766.1"
                     /translation="MAIQELIKTEKLLFEKPDSVSASKPFTAESLPNFPAASRLIGVV
                     SIDEITAENFSSLDSLIQEIKDIFSRKNEFDNFNSEEIFEITLKQVNKKVTKMIEGEK
                     LIIKPNKFHCVIGLLTPDSKSFNKKFNLYLSAFGLTNAHLIHKTPSGDYKFIDIVASP
                     AGSAGGASPAADEATEPNQSATEDKTPLKFFTNVVSGKINSTDTALLTTANLTDFIST
                     GEIKKIISTLPPVSSIQHISNLLSEVTNPISIAAVIAQPASRERKQPGLIGVATGSSQ
                     DSMNVLVSTESATEKLLTPAMSLNIKKLSSALTDKLRSLTGGQNKQTDGRSESRKRDN
                     FLVLSVKFLSQAGSNLVYILVNIFKGIINFLGRVNYRKLTDLNYVKNSAAASVQNAKN
                     NLAENFKNIISWYGRLPKIRKYLLTAGIILIFIFTSSLFLQNRQKNLEAEALAYTTSL
                     NEILDKKDKAEASIIFKEEDKAKSLLDETVALIADLPQNTNDRRDQAQILLVQIENLN
                     DQLKHLIKIDNPAVIADLAPFDPSVNIQTFSVNKNKIFVTTSNQSELYQINLDRDNNI
                     EKLELAGNQEVLLKTIAKDEIIYFYSDQNNLLKLDTINLELASLNLDIPADAKIEDLA
                     IYNNRLYLLDSNNQQIYRYNQAGNDFAVSKTNWLRDNTANISQAVSLAIDANIYVLKS
                     NGLVEKLLGGSRFLPAFSLSSVEPSLDNPSKIRTLENSEYIYVLEPANNRLVIFNKGG
                     KFLNQYVSEKFKDLKDVYIDEANKTAYLINGTKIIKIELSHF"
     gene            5234..5899
                     /locus_tag="VGA49_02990"
     CDS             5234..5899
                     /locus_tag="VGA49_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664767.1"
                     /translation="MLEQLFGSKTRTRLLKLFLDNPAKVYFVRELTRVTKTQINSIRR
                     ELKNLSDLGILKVVETTLPDAKNKKNTRRPPGRKSGIKAKKFYQVNYGFVLLPELKSI
                     FSKSALLIERDVAYKLRNVGKINYLVLTGFFTGQKDLATDMLVVGNVNAIKIKKVVDS
                     IQKDIRREINYTILSADEFSDRKDIGDRFIFDILLNRNIVVIDNLKPPTKKDQPTDEK
                     LKF"
     gene            5880..>7086
                     /locus_tag="VGA49_02995"
     CDS             5880..>7086
                     /locus_tag="VGA49_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX9664768.1"
                     /translation="MKSSNFKKQFIIILLVIGAGICWPVIKVLAQPLVRIFDVNGFNL
                     EQEFNPFVNLDVTGLSVAVLDFDGNGLNEVAVSAGRGERPLVRLFNYQGWLLTEFPAY
                     AENYQLGLKVVAADLDGDGRDEILTGPLDGGSPHLRIFQYQPDGFVLINEFFPFDKDL
                     RNGLNLAVGDVNNDSQPEIIVSQNLGGEPKVRIYNKKLSLLNEFEAYSNNFKGGVNLA
                     VSDLDRDGQAEIITGAGSGGGPHVRVFNDQGQVKNQFFAYQENFKGGVSLAVADLDHN
                     PSPEIITGAGFGGGPHIRIFDRLGQPKLISNFFSFDHDFRGGVEVAVGDVDNDGLQEI
                     IAATAYLPKLNPGSYRYIEVDLSQQKLFAYQRGDLVKEFLISSGTGWFPSPTGNFRVY
                     RKRESARMTW"
BASE COUNT         2031 a         1318 c         1625 g         2112 t
ORIGIN      
        1 agaaccgcgg tttattgtga tggaatatga tggaggagac tctaaagaaa aacctctggt
       61 gctgattggc aagggaatta cttttgatac cggcggttta aatttaaagc caagcgacgg
      121 aatatatcag atgcatctgg atatgtcggg cggggcggct gttttatatt cagttttatt
      181 agccgccaga ttaaaattaa agaaaaatat tgtcggtttg ataccggccg ctgaaaacat
      241 gatttccgga tcaagttacc ggccgggaga tattttaaaa accatgtccg gaaaaacagt
      301 cgaagtttta aacactgacg ccgaaggccg tctggtttta gccgacgccc tgacttacgc
      361 cgggaaattt tataaacccc gactggttat cagtgtcgcc actttgaccg gggcggcgct
      421 ggtggctttg ggacagtatg cctcggctat tttttcaaaa gatgagtcat tattgaaact
      481 gttgcgtaat ttgggcgaga agagcgggga ctatgtctgg cctttgccgc tttgggaaga
      541 atttgagaaa gaaatgaaag gcacttttgc cgatttgaat aatctgggca aatcaaaata
      601 tggcggcgcc agccagggag cggctttttt ggcccatttt gccaaaaaat atccctggct
      661 ccacattgat atcgctcccc ggatgactgc cggcgaaggt gaatatttgg ccaaaggcgc
      721 ggttggcgcc ccggtccggc tgctgatcag gtttttagag acttattaaa tattttttca
      781 gtaaatgaaa aaagaaacca ttattatttc tttaggcggt tcaattataa ttcctgacaa
      841 gcttgatatt gtttttttac aaaatttcaa aaaattaatt ttaaaacaca tccggcaaag
      901 ccggcgtttt tttattgtga ccggcggggg acagaccgcc cggatttatc aaaaaggagc
      961 taaaaaaatt gaccaggtta gcgccgatga tctggactgg ctggggattc acgccagccg
     1021 tttgaacgcc cacctactgc ggactatttt taaaaaacag gcccatccgg tagttatcaa
     1081 agagccggat gtttataaaa gaatcaaaga ggacattatc attggcgccg gctggcagcc
     1141 gggctggtca actgattatg tctgcgctta tttggccaga acttataaaa ttaaaaatat
     1201 cgttaatctg tctaacattg cctatgttta tgacaaagac cccaataagt ttaagcaggc
     1261 tcaaaaagta aaaaaaatat catggcctga ttatcggaaa atagtcggca ataaatgggt
     1321 tcctggcgca aattttccgt ttgacccggt ggctgctaaa ttatcccaaa aactgggttt
     1381 aaaagtgtca attttaaaag gcggtgatct caaaaatttt gatgattatt taatgggcca
     1441 taagtttaag ggaacagtta taaattagtc atatttgaca tttttttagt attgcgataa
     1501 tattatttaa ttaaataaac ccacagacag taagggtcca tttccagggc ttaaatatcc
     1561 caccttacct agggaaaatg ctagtttttc gtctgtggtt taatatccac agatttttat
     1621 tttaaccaaa cctatgcctg tcaataaaaa gcaaaaagaa gaaatcgtca gtgatttagt
     1681 cgataaactg aaaaaatcaa aatcagttat ttttgttaat tttgaccagg tgactgtttc
     1741 tgaaattgaa gagtttcgcc agctttgccg ggagcaggcg atagactata gcgtctccaa
     1801 aaagactctg ctgaaaatcg ccatggacaa gggcaatttg aaagaagttg acccgctggc
     1861 ttttgaaaat ggcgttggga ctttattcag ttatgacgac gaagtggccc cggccaaaat
     1921 tgttaaaaat ttttccaaac tgcatgaaaa tttaaaaatg atcggcggca ttctggcttc
     1981 caatccggag ggtcaaagat atttggctct ggctgaagta gaaaatctgt ctatgattcc
     2041 gtccaaagat gaactgctgg ccagggttgt cggcagtctt aacgccccgg ttgccggttt
     2101 agttggcgtc ctgtcgggta atttacgaca attagtttat gttttaaacg ctattggcga
     2161 atcaaaacaa taatttatta attaaataat taaaatatat ggctgaagaa caaaaacaag
     2221 caaagcaggc tgaacaaaaa cctgctgaga agaaaaaaga agaaaccgca aaagctgaag
     2281 aaaagccagc tgaactaaag gcggaagagt ctaaaaaaga agtttccgtc tccgccaagg
     2341 cttcggtgga catcgaggtt cctgacaaat tcaaagcttt agtggagcag attgaacagt
     2401 tatcagtcct ggacctggca gaactggtca aagttttgga agaaaagttt ggcgtttcag
     2461 cggccgctcc ggtcgccatg gccgccatgc cgggcgccgg cggtgaagcc gtcccggctg
     2521 aagaaaagtc agaattcagt gttgagctga ctgaagccgg ttcaaacaaa atcgccgtga
     2581 ttaaagcgat cagaggagtg attgaaatgg gactgaaaga ggccaaagat ttgaccgaag
     2641 gcgctcccca gataattaaa gaaggcgtta aaaaagaaga agctgaagaa atgaagaaaa
     2701 aactggaaga agccggcgct aaagtgacgc taaaatagat gtttacaaaa aaagccggct
     2761 tcagattcct ggaaccggct tttttattgt taaaaatgac ttaattcaat ttttattatt
     2821 ttggttccat taatcagata agcggtttta ttggcttcat caatatagac atctttcaaa
     2881 tctttaaatt tttcgctgac atactgattg aggaacttgc ctcctttgtt aaaaattacc
     2941 aggcggttat tggctggttc taaaacataa atatattctg aattttccag agttctgatt
     3001 ttagacggat tatccaaaga cggctcaact gacgataagc tgaaagccgg caggaacctg
     3061 gagccgccca gtagtttttc caccaggcca tttgatttta ggacatagat attggcgtca
     3121 atggccagcg agacagcctg actgatattg gcagtattgt cccttagcca gtttgtttta
     3181 ctgaccgcga aatcatttcc cgcctggttg tagcggtaaa tttgctggtt attgctgtcc
     3241 agtagataaa gccggttgtt gtaaattgcc aaatcttcaa tcttagcgtc agccggaata
     3301 tcaagattaa gactagccag ctctaaatta atggtgtcca gctttaagag attattctga
     3361 tctgaataaa aatagattat ctcatcttta gcgatagttt ttagtaaaac ttcctgattt
     3421 ccggccagct ccagtttttc aatattgtta tcccggtcaa gattaatctg ataaagttct
     3481 gactgattgg acgtggttac aaaaatttta tttttattaa ccgaaaaagt ctgaatatta
     3541 actgatggat caaaaggagc cagatcggca atgactgccg gattgtcaat tttgatcaga
     3601 tgtttaagct ggtcatttaa attttcaatc tgaaccagca gtatttgagc ctggtcccgg
     3661 cgatcattgg tgttttgggg caggtcagct attaaagcga ctgtttcatc cagcagtgat
     3721 ttggctttat cttcttcttt gaaaattata ctggcttcgg ctttatcttt tttatccaga
     3781 atttcattta aactggtggt gtaggccaga gcttcggctt ctaaattctt ttgcctgttt
     3841 tgcaggaaca gactgctggt aaaaataaaa attaaaatta taccggcagt cagcagatat
     3901 tttctgattt ttggcaggcg gccgtaccag cttattatat ttttaaaatt ttcagccaga
     3961 ttgtttttgg cgttttgaac tgaagcggcg gccgaatttt taacataatt caggtcagtc
     4021 agtttacggt aattaactct gccgaggaag ttgattattc ccttaaaaat attaaccagg
     4081 atataaacca ggtttgagcc ggcctggctt agaaatttga ctgataaaac gagaaaatta
     4141 tctcttttgc ggctttcgga ccggccgtcg gtttgtttgt tttggccgcc ggtcaggctt
     4201 ctcaatttat cagtcagcgc cgaacttaat ttttttatat ttagactcat cgccggagtc
     4261 aacagttttt cagtggcgct ttcagtgctg accaaaacat tcattgaatc ctggctggaa
     4321 ccggtggcga ccccgatcag tcccggttgt tttcgttccc gggaagccgg ctgggcaata
     4381 acagcggcga ttgagatcgg atttgtcact tctgataata aattggaaat atgctggatt
     4441 gagctgaccg gcggcaaagt cgaaattatt tttttgattt caccggtgga aatgaagtca
     4501 gtcagattag ccgttgttaa aagcgcggtg tcagtcgaat taattttgcc gctgacaaca
     4561 ttggtaaaaa attttagcgg agttttgtct tctgttgccg attgattcgg ttcggttgct
     4621 tcgtcagcgg cgggactggc cccgccggca ctgccagcgg gactggcaac tatgtcaata
     4681 aatttatagt cgcccgaggg agttttgtga attaaatgag cgtttgttaa gccgaaggcg
     4741 ctcaaatata ggttaaattt tttgttaaaa cttttggagt caggtgtcag cagtccgatg
     4801 acgcaatgaa acttgtttgg tttaataatt aatttttcac cttcaatcat cttagttact
     4861 tttttattga cctgcttcag ggtgatttca aagatttcct ctgaattaaa gttatcaaat
     4921 tcatttttcc tggaaaaaat atctttgatt tcctgaatca gactgtcaag cgagctgaag
     4981 ttttctgccg taatttcatc tattgaaaca accccgatta gccggctagc cgcgggaaag
     5041 tttggcagac tttctgctgt aaatggtttg gaagcgctga cgctgtctgg tttttcaaat
     5101 agtaattttt cagtttttat tagttcctgg atggccatat aacttgaata aatttttggt
     5161 atagtttaga ctatagttag attatgacta tattagataa tcataatact aaacattaat
     5221 ttttacaaat ccgatgcttg aacaattatt tggttcaaaa acccgcacca gacttctcaa
     5281 gctttttctg gataatccgg ccaaagttta ttttgtcaga gaactgaccc gggtgaccaa
     5341 aactcaaatt aattcaatca ggcgggagct gaagaatttg tctgatctgg ggattttgaa
     5401 ggttgttgaa accactctgc cggacgctaa aaataaaaaa aacacccggc ggccgcccgg
     5461 gcgcaagtca ggcattaagg ccaaaaagtt ttatcaggtc aattatggat ttgtcttatt
     5521 accggaactc aaaagtattt tttcaaaatc cgccttattg attgaaagag acgtggctta
     5581 caaattgaga aatgtcggta aaataaacta tctggtttta accggttttt ttaccggcca
     5641 gaaagatctg gcgactgata tgctggttgt cggcaatgtt aacgccatta aaatcaaaaa
     5701 agtagttgat tcaattcaga aagacatcag gcgggaaata aattacacga tattatcggc
     5761 ggatgaattc agcgacagga aagatattgg tgatcggttt atttttgata tactgttaaa
     5821 caggaacatt gtcgttattg ataatcttaa acctccaaca aaaaaagatc agccgactga
     5881 tgaaaagctc aaattttaaa aaacagttta taataattct tttagttatt ggcgctggta
     5941 tttgctggcc ggtaattaaa gttttggctc agcctttggt tagaattttt gacgttaacg
     6001 gttttaattt ggagcaggaa tttaatccgt ttgtcaattt ggatgttact ggtttatcgg
     6061 tggctgttct tgattttgac ggcaacggcc tgaatgaagt ggctgttagc gctggccggg
     6121 gagaaagacc gctggtcagg ctttttaatt atcagggctg gctgctgacc gaatttccgg
     6181 cctacgctga aaattaccag ctcggtctca aagtggtcgc ggctgatctt gacggtgacg
     6241 gccgggatga aattttgacc ggaccgctgg acggcggctc gccccatctc cgaattttcc
     6301 agtatcagcc agatggtttc gttctgatca atgaattctt tccttttgat aaagatttaa
     6361 gaaacgggct aaatctggcc gtgggcgatg ttaataatga cagtcagccg gaaattatag
     6421 tcagtcagaa tcttggcgga gagccgaaag tcagaattta taataaaaag ttgagtttat
     6481 taaatgaatt tgaggcttat tcaaacaatt tcaaaggggg cgttaatctg gccgtcagcg
     6541 atttggaccg ggatggccag gcggaaataa ttaccggcgc cggatccggc ggcggacctc
     6601 atgtcagggt tttcaatgat cagggccagg ttaaaaatca gttttttgcc tatcaggaaa
     6661 attttaaagg aggcgttagt ctggcagtgg ctgatcttga tcataatccc agtccggaaa
     6721 ttattaccgg agctggtttt ggcggcgggc cgcatatcag aatatttgac cggcttggcc
     6781 agcctaaact gatttctaat ttttttagtt ttgatcatga ttttcgggga ggagttgaag
     6841 tcgctgtcgg agatgttgac aatgacggcc ttcaggaaat catcgcggcc accgcttatt
     6901 tgcctaaatt gaatcccggc agttaccgtt atattgaagt tgatttaagc caacagaaat
     6961 tgtttgctta ccaaagaggg gatttagtca aagagttttt aatttcatcc ggcaccggct
     7021 ggtttcccag tccgactggt aattttaggg tttaccgcaa gagagaatcc gcccggatga
     7081 cctggt
//
LOCUS       DASWHZ010000148         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_650509, whole genome shotgun sequence.
ACCESSION   DASWHZ010000148 DASWHZ010000000
VERSION     DASWHZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650509"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGA49_03000"
     CDS             <1..267
                     /locus_tag="VGA49_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664769.1"
                     /translation="KNKMSYKLAVMEADKLFDHVLKKMYFPGSTLGERLKAACYKYDK
                     LREIWWAHKVRNQIVHEATYDLNYKTAQGALKTFKKGLEELGIL"
     gene            381..1010
                     /locus_tag="VGA49_03005"
     CDS             381..1010
                     /locus_tag="VGA49_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX9664770.1"
                     /translation="MLIILGLPGVVSAQSFLDDEISDSLINSQTKVLINLQQKSWKTF
                     VFESSVNLIDSGQLTYSWDFDDGQTADGRGVEHKFKKSGDYLVAVVITDDQGNKLTEL
                     IQVEISFFNPGNWKVWSLVILLIILIIMALIFIGYRPKKEAVSQLIDKTKKIIQSEKS
                     ALTDKPKQEPVGPKLESDKSADEQDWEEYLETESDESEELAEELESLEK"
     gene            1050..1865
                     /locus_tag="VGA49_03010"
     CDS             1050..1865
                     /locus_tag="VGA49_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX9664771.1"
                     /translation="MKNKIVIVSLILFFFIIALGVYLMLPQEQTELITVTTTNEAVAE
                     HFESDLIVKTVRTVNDIEDQPADNVSQAQEIVQEVTLEIPVKKILEVPFTSQAPYEDW
                     SQPYQDACEEASAIILAYYLSDQNLNKDIANEEILNLVDWQNSNFGDFKDTTAAKTAE
                     MIEKYYGYKTKVAYDISINDIKLEIAKGNPVIIPAAGQLLGNRYFTPPGPVYHMLVVI
                     GYDDDEIITNDPGTKRGAGYRYDYDVFYNAIHDWTGSYETINEGRKAMIVVTK"
BASE COUNT          647 a          354 c          437 g          558 t
ORIGIN      
        1 aagaataaga tgagttataa actggcggtc atggaagctg acaagctttt tgatcatgtt
       61 ttaaaaaaaa tgtattttcc aggctcaacc ctgggagaaa ggctcaaagc ggcttgctat
      121 aaatatgaca aactccgtga aatctggtgg gcccataaag ttagaaatca gattgtccat
      181 gaagcgactt atgatttaaa ttataagaca gcccagggag ctttaaaaac gtttaagaaa
      241 ggcttagaag agcttgggat actttgaaaa tagtcactag tcactagcca cgagggggtt
      301 atactaatga ctagtgacta atgactaatg acttatcaat gaatttaaac aaaagcagac
      361 tattattccc ggggctttgt ttgttaatta ttctaggcct acctggggtg gtcagcgccc
      421 aaagctttct tgatgacgag attagcgatt cgctgataaa ttctcaaacc aaagtcctga
      481 tcaatcttca gcaaaaaagc tggaaaactt ttgtgtttga atcaagcgtt aatcttattg
      541 acagcggcca gctgacctat agctgggatt ttgacgacgg acaaacagcc gacggccggg
      601 gagtggagca taaatttaaa aaatcaggcg attatctggt ggcagtggtt attaccgacg
      661 atcaggggaa taagctgacc gaacttattc aggttgagat cagttttttc aatcccggca
      721 actggaaagt ttggagcctg gttattttat taattatctt gattattatg gctttaattt
      781 ttatcggtta tagacctaaa aaagaagctg ttagtcagct gattgacaaa actaagaaaa
      841 tcattcaatc tgaaaagtca gcattgactg acaagcccaa gcaagaaccg gttggcccga
      901 aattagagtc agataagtca gcagatgagc aagactggga agaatattta gaaactgaat
      961 cagatgaaag cgaagaactg gccgaagaac tggaaagctt ggagaaataa tatctaatta
     1021 aagcgaataa aagctaatat acgctaacaa tgaagaataa aatagtaatt gtcagtttaa
     1081 tattgttttt ttttattata gccctggggg tttatttgat gctgccgcaa gagcagactg
     1141 aattgataac tgttactacc actaacgaag cagtagctga acattttgag tctgatttga
     1201 ttgttaaaac agtcagaaca gttaatgata ttgaggatca gccggctgat aatgtcagtc
     1261 aagcacagga aattgttcag gaagtaactt tggaaattcc agttaaaaaa atattagaag
     1321 tgccgtttac ttcccaggcg ccttatgaag actggtccca gccgtatcag gacgcctgtg
     1381 aagaagcgtc agccataatt cttgcctatt atctcagtga tcaaaattta aataaagata
     1441 ttgccaatga agagatttta aatttggttg actggcagaa cagcaacttt ggtgatttta
     1501 aagacactac tgccgccaaa accgccgaga tgattgaaaa gtattatggc tataaaacaa
     1561 aagtggctta tgacatttca atcaatgaca tcaaactgga aatcgccaaa ggcaatccgg
     1621 tgattattcc ggccgctggg caattattgg gcaaccgtta tttcaccccg cccggcccgg
     1681 tttatcatat gctggtggtt attggatatg atgatgacga aatcattacc aatgatccgg
     1741 gcactaagcg cggcgccggc tatcgctatg attatgatgt tttttacaac gccattcacg
     1801 actggaccgg cagttacgaa actataaatg aaggccggaa agcgatgatt gtggttacca
     1861 aataatatgt tgccatatat agaaatcatt acaattaacc tcggccccat cacccttcag
     1921 ttttggggtt tgatggtttc tttgggattt atcgtcgcta ttattttagc ttattttctg
     1981 gcgaaaaaaa gcaaca
//
LOCUS       DASWHZ010000149         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_281858, whole genome shotgun sequence.
ACCESSION   DASWHZ010000149 DASWHZ010000000
VERSION     DASWHZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281858"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /gene="ruvX"
                     /locus_tag="VGA49_03015"
     CDS             complement(<1..174)
                     /gene="ruvX"
                     /locus_tag="VGA49_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9664772.1"
                     /translation="MLNILGIDYGSKRVGLALGDPEKKMAFPFEVIENKNRKYLLEKI
                     KDVCLRENIKEVVV"
     gene            complement(237..1007)
                     /gene="map"
                     /locus_tag="VGA49_03020"
     CDS             complement(237..1007)
                     /gene="map"
                     /locus_tag="VGA49_03020"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX9664773.1"
                     /translation="MITIKKPEEIEILRQSGRILASTLQQVVSQVKPGVLTSDLDQFA
                     EKLIRGQGGQSSFKNYVSHGSDPFPSSLCTSINEEIVHAPAIPSRPLKDGDIIGLDLG
                     VNYKGFFTDMAVTVGVGRISAEAKNLMKATKKSLELALKQVKPGNYIHDISNAVQRYV
                     EKQGYGVVRELVGHGVGYQVHEDPRIPNYLPDNGEPERIELKEGMVLAIEPMVTAGHW
                     KIKTLEDGWTAVTIDGKLSAHFELTVAVTAKGYEILTK"
     gene            complement(1074..>1524)
                     /locus_tag="VGA49_03025"
     CDS             complement(1074..>1524)
                     /locus_tag="VGA49_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEX9664774.1"
                     /translation="APDEITNQLMREEVEKADSRGFVLDGYPRNQVQLEAMKKFAQPT
                     HVFLINIPEEESIRRIAGRRICDSCGADYNIYTKPPKQDEICDKCGARLHQRHDDHPD
                     GVKQRLKIYHNDTEPLLKYFEEQDILRKIDGLGTVDQVFERVITAFQ"
BASE COUNT          416 a          382 c          271 g          455 t
ORIGIN      
        1 aacaacgact tctttgatgt tttccctaag acaaacgtct ttaatttttt ccagaagata
       61 tttccggttt ttattttcaa tcacttcaaa cggaaaagcc atcttttttt caggatcgcc
      121 cagagccagg ccaactcttt tggatccata gtcaatccct agaatgttta gcataaatgt
      181 aacaagggta aattaaggtt ataatcctca tcaatcataa tcaaccttga tatcccttac
      241 tttgttaaaa tttcatagcc tttagctgtt acggccactg ttaactcaaa atgggctgat
      301 aattttccat caatagtcac cgctgtccag ccatcctcta aagtttttat tttccagtgg
      361 ccggccgtga ccattggttc aatcgccaac accatcccct ctttcaattc tattctttcc
      421 ggctccccat tatcaggcag ataattggga atccggggat cctcgtgaac ctgatagcca
      481 acgccgtggc caaccaactc tctgacaact ccataacctt gtttctcaac atatctctgg
      541 actgcattgg aaatatcatg aatgtagtta cctggcttaa cctgcttcaa agccagctcc
      601 agcgattttt tggtagcttt catcaaattt ttggcttccg cggaaattct accaacgcca
      661 actgtcaccg ccatgtccgt aaaaaatcct ttataattaa cgcctaaatc aagaccaata
      721 atatcgccgt ctttcagcgg ccggctgggg atagccggag cgtggacaat ttcttcatta
      781 attgaagtac ataaagaact gggaaatgga tccgatccgt gcgaaacata atttttaaat
      841 gacgactggc ctccctgccc cctaatcagc ttttcagcga attgatccaa atcagatgtc
      901 aaaacaccag gtttgacctg gctgacgact tgctgaagcg tgctggccaa aatccggccg
      961 ctctgcctca aaatttcaat ctcttccggc ttttttatgg taatcatagc aacttaacca
     1021 taacaaaaaa gacccggttg ggtcaattgt aataaaatta aaaatttaat taattattga
     1081 aaggccgtta ttacccgctc aaaaacctga tcaactgtcc ccagaccgtc aattttacgc
     1141 aaaatgtcct gctcttcaaa atatttcagt aacggttcgg tgtcattatg ataaattttt
     1201 aaccgctgtt taactccgtc aggatgatca tcatgacgct gatgaagcct ggctccgcac
     1261 ttatcacaaa tctcgtcttg tttaggcggc ttggtgtaaa tattataatc cgccccgcag
     1321 ctgtcgcaaa ttcgccggcc ggcaattcga cggattgatt cttcttccgg aatattaatc
     1381 aaaaaaacat gggtcggttg agcgaatttc ttcatagcct ccagctgaac ctggttgcgg
     1441 ggatacccat ctaaaacaaa tccccggctg tcggctttct ccacttcttc tctcatcagc
     1501 tgattggtga tttcatcagg ggcc
//
LOCUS       DASWHZ010000150        13087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_654162, whole genome shotgun sequence.
ACCESSION   DASWHZ010000150 DASWHZ010000000
VERSION     DASWHZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654162"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VGA49_03030"
     CDS             <1..221
                     /locus_tag="VGA49_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664775.1"
                     /translation="EVKRKNDESFESFLRRFNKRLIQSGKLYHARKIRFYQEEPNRNK
                     SKKDALRRQKIRSKKEYLRRIGKLQEDF"
     gene            246..1880
                     /locus_tag="VGA49_03035"
     CDS             246..1880
                     /locus_tag="VGA49_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9664776.1"
                     /translation="MKDKLNSGKKQKVAIIMALFIIGGFLLSANFISAQVTVPAGPGL
                     DVGLEPTEAIGLPATDIRIIIARIIRVILGFVGIIGVIIVLYGGYTWMTAAGNEEKIS
                     QAKKILLNGGIGLIIVLSAFSITSFIISRLQAALVGEFAPTPGAGPFVGGGLGVGIIE
                     SHYPARNATGIPRNTKIIVTFKEKMLLESLVNDNGTPDDLTDDFIQPESVRIRRTVDD
                     RSAGPFVVNVRAAYTDDLRSFVFDPFDLLGTATENVSYTVALTDQVKRSNGQSAFGGL
                     GSYDWSFEVSTLVDSEPPQVESVIPVADSVNPRNIIIQINFNEAMDPTTVTGSTGVGF
                     NKLVVYNSTDQSPVSGTFNVSNQYKTVEFISNDKCGVNSCGEDVFCLPGDKEILVTVK
                     AASLSEAPPEALFPYNGVVDVVGNSLDGNQDNAADGPPADDYFWQFQTNNTIDLTPPI
                     IESLGPDKFSAGVALDEVVAATFSKLISASSLNTSNLVLEGINYWTGARNNMIEQKTT
                     GLIFHDLFSQDTPYNPFVTSGVRDIYQNCYQPCIGP"
     gene            1944..7001
                     /locus_tag="VGA49_03040"
     CDS             1944..7001
                     /locus_tag="VGA49_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX9664777.1"
                     /translation="MFKNKNKILLIAWLLIVFSVFLVTSNVLAQVDVGVQPLEEIGLP
                     TQDIRITIANIIRIALGFVGIVGVILVLYGGFVWMTSGGNEERVSQARKILTGAAIGL
                     VIVISAYAITSFIISRLAEVTGAPQAGVSSEFPGFPPGFSPGEAFLVRSASPVGAVPI
                     RNVKVRVLFNRSVDQVSVAGNITVAAAADGGLVAGDYNVSGSTVEFTPQAACPEPNQN
                     LRCFAENTEFVVNIISGADGVKSSDGRELVCGLAGSNCRSSFTTGSLIDTRPPTVDLV
                     SPVAGQSLSVDDIILLEALARDDSGIAYVEFYADDVLIDTAAPSLSVSPADYTAQANW
                     DTTGLILGSAHTIQAIAFDIDDHQTESRTISVTVRAAHCFDGLQNQDETNIDCGGADC
                     GSCDGEACVQNSDCASGQCVDGFCLRLPLITRVDPADGAVGNYVSIIGQAFGSAAGEV
                     VFMGDPFNPDDDRTASLALCAAAWRDSQVVVTVPAGAVSGPVRLTTAGNLFDQTNDTR
                     GWQGDFNVNDTVRPGLCSISPTRGEYGAVATIEGNNLGDQTASLRFKETEAESIASWT
                     DVKIDGIAIPNINPEEVPVFATVNNLRSNSLNFTVQTSTTAPRIDYVDPVAGPVGQYL
                     TIFGTNFGGQTGQVFFVSPDGQEILADTSFPENCADKFWHDSYINVKVPNVPIGDYQI
                     KAVKGTQESNLVAFKVNNAPRTPGICRLSPDNGPVGIEMTIYGERFGSDANKVRFFRG
                     QENGSIIAWEDRQIRILVPPLAQTGPVEVIDSSGTLSNSVNFAVGFCSSTTCSETEDC
                     CGDGSCREAGSCQVVPPLCAYSWTFTTGRGGLNDPCQTDADCLAGLACDPLSKTCQEG
                     FPRVVEDQECLETPQSPSPWKSSTDACVNAGIAARFNMNMKDVTLNPANIILAQCNTG
                     ARFQADDCDQPVGLNRVEINQVIDIDGNIQEGFVAVPGASLRANTWYQVTLTTGLESE
                     TNRSLSQDYLWTFKTRDDTTPCEISHIQVSPQYKTLTYKFEEQGYLAQPTAANCNTLN
                     PDSYAWNWSSTDPSRAAVVNNVTSKTQATALNETESDSPVQVTAAIPTEQKSDFGELT
                     VDFGDPEVILYWPNCRAACVNASIGAKFNVDLQANTLNNNTLRLYACDNENCTNLDQL
                     ALNNIVYNPLDLEVEFMTAAALEVNKFYRAVITSGVTSTSNVTLTNLNYSTTGGALDG
                     FSWIFKTKPDGSLCAVDQINVRPGYKELYLIGQSQGYYSQAIGSPDECNPGGQRLNPY
                     SYNWDWDSTRSSVATVSNSDLDPVDGRIDPKQAARSRGEGETDIQAGTQGQVGQGRLK
                     LVCGFTADDQCPAPATIETYGVDDNSCCAPRPKIVRTRPIDQAVSVCRNAVIEADFDQ
                     LMELGSILGNVLVEANYGDQPCPAGGQSRSIWGRLVQFFKNIFSRDIQAQAADNWCAV
                     AGRTSLFQENDQTSLQYYLEQPLEPGRQHRVTIQGDPNVSDQNKEGVLSLSGLTMNGD
                     YQWSFTTGDEICDIDYAEVTISPPGEVGVYDLLTCAGRDDCPGDQDSSTPGNQHFYKA
                     VARDDSGVILPANFEWVRTGDQVINLSATTDQQIAVTPSAANGKPLVTVVARGIPPTP
                     GMASKTVNVFVFLCENPWPSLAEFPYRDLTTNFSTFYCRDRGAAGFSDDYPQFSDTPA
                     VTPTDPRIIKEFLFIVE"
     gene            7004..9124
                     /locus_tag="VGA49_03045"
     CDS             7004..9124
                     /locus_tag="VGA49_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX9664778.1"
                     /translation="MTRADLIKNICKFSLGLLIISAFVLTGSPAQAAEAIGIRILANP
                     GRLSPFGWYFQNVPNPGQPEPLLVNGYPAIREGRTVYVAATNQVGDALYGNIYLISYS
                     EDASQSTIDIFNQLLENWDFNINVNDLVVREQLRRDMKRAGDMSDVSGLLERYRRVNG
                     NYPLLQAGTYIRGLSFSVWPSWQATLANLLASALPQDPLNRLIGCQDPYDQTTCWDEV
                     NQQFQCSPESRTYAYQVDDNGDQASLYTNYEYTGPGSWRTGEYGLLTSQFCFTNESAF
                     LNDEDGDGILNSADNCPTVYNPDQADFDRDGLGALCDLCDLDPSNDIDRDFVCGGVDN
                     CPKNFNPDQTDADADGIGDACDIMVCGNAIKEGDEVCDGLGSIPANSTCLNDCSGWTC
                     NEGYSRFDNICDIDTDGDLVADSLDNCSNLYNPGQLNSDNDIYGDACDWCPDDSDNDI
                     DGDGYCAGPRYSGPRLGANDICPTVADPEQANTDGDELGDACDQCPDDPANDPDDDGV
                     CAGARFNSPKLAGSDNCPAVANQDQADFDRDGIGDACDPQTCGNNIIEAGEKCDQNQG
                     LAGIICTGQEELYCSVDCQISCTGQGQPVYAISGSAWSSNIGWINLSPSNGGVTISLT
                     NTFVGSAWSANVGWINMSGATVNLDGTVHGDILGNDYVGVINYDPSRSGVRINSADGT
                     FSGFAWGENIGWIDFNGVSTSWKP"
     gene            9276..9932
                     /locus_tag="VGA49_03050"
     CDS             9276..9932
                     /locus_tag="VGA49_03050"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664779.1"
                     /translation="MPEGQPTPTAPTIPPTPPKPIPPREPEPTPSIVPPPVGIEDILS
                     GTEAAGPAGPGSPTPPTSDQGQPPDIIPEYPETSFFLKYRLLIIIGVLVILFVAIALI
                     ILWVTTRQPVFQQPVNINAIDQPPVTPPPAVPVDSDRDGLTDEEEKELGTDLNNPDTD
                     GDGLTDFEEVRVYKTDPLNPDTDGDGFLDGDEVQAGFDPTQGGGARLLDLEREINNIR
                     "
     gene            9942..10955
                     /locus_tag="VGA49_03055"
     CDS             9942..10955
                     /locus_tag="VGA49_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005040034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664780.1"
                     /translation="MFGRNKKEKSIESSADQPAGPAPGNIRTIPDQFLYKEEKKKTGG
                     LVIIIIGALVLIVLVAGGVWLLTGSFGQPTEVTRVENVNLEVKPPVNENLNVNLNVNA
                     NLNSNVNANLNANANVNAGLPPVIPGLLASSLDSDQDGLTDVEEELYGTNPNLPDSDG
                     DGHIDALELMNLFNPAEPAPAELPGSNLVELYSNPSFDYRILYPSRWIARPIDTELKE
                     VIFSSETGEFIEVLVSENPEGLSVLNWYLQQSPTVTPSQVQSVVTKNNIRGIQSVDGL
                     TVYLVKPVDSSITGEAEAKQYIYAISYNPGTRPELNFKSTLKMMYNSFELGQLGQPAP
                     SSP"
     gene            10962..11405
                     /gene="ybeY"
                     /locus_tag="VGA49_03060"
     CDS             10962..11405
                     /gene="ybeY"
                     /locus_tag="VGA49_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9664781.1"
                     /translation="MILLAVNQDYLKEKKLAKAWFEEITQATSGCLNLKKNYFVSLAF
                     IDQKKIKMLNNKYRLKNQVTDVLAFSYQETDQEAPDRTYLGEIFICYPQAEKQALEHR
                     QPVEEEIKTLLIHGLLHLFGHDHATEADHRKMKKLEDKIRKINLN"
     gene            11407..11760
                     /locus_tag="VGA49_03065"
     CDS             11407..11760
                     /locus_tag="VGA49_03065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9664782.1"
                     /translation="MDNQKLRKSFKYAWRGLADILKTEQNFQIELASGLMVVVLMFYF
                     QVSLLEAAILILTILTVLVFEIVNTVIERLVDLIKPQIHQQAGRIKDMTAAIVLVASI
                     GAAIIGLIIFLPYFF"
     gene            11937..>13087
                     /gene="ftsA"
                     /locus_tag="VGA49_03070"
     CDS             11937..>13087
                     /gene="ftsA"
                     /locus_tag="VGA49_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9664783.1"
                     /translation="MRDEIITGLDVGSTTIRIVVGQKIVDSNNEEKLHIIGVAETSAE
                     GISKGVIVSIDDAVSSISSCLEKAERMAGVPIEQAYVGISGSHILSQGSKGVIAVSKA
                     NGEINEDDIERVIEAAQAVATPPNYEILHVIPRNFTVDNQMGIKDPLGMTGVRLEVEA
                     QIIQGLSSQVKNLTKTIYRTGVDIEDLVLAVLAAAESVLSKRQKELGVALVNIGGSTT
                     SLVVLEEGDVLHTSILPIGGGHITSDVAIGLRTSIDVAEKVKIEYGSAVPNNINKKDE
                     INLAEFSSGDESTVSRKHVAEIIEARLEEIFELVDRDLKKINRSGLLPAGVVLTGGGA
                     RLEGIVEVAKKKFRLPASIGYPTEMASAIEKVNDPIFSAALGLVIWGSQL"
BASE COUNT         3292 a         2898 c         3406 g         3491 t
ORIGIN      
        1 tagaagttaa aagaaaaaat gatgaatcat ttgaaagttt cttacgacga ttcaacaagc
       61 ggctgattca aagcggcaaa ctttatcacg cccgtaaaat aaggttctat caggaagagc
      121 ctaaccgcaa caagagtaaa aaagacgccc tgcggcgcca aaaaatcaga tctaaaaaag
      181 aatatttacg ccggatcggc aaattacaag aagattttta attattattg ataatgagct
      241 tgattatgaa agacaagctc aattcaggta agaaacaaaa agtcgcgatt ataatggctc
      301 tttttataat cggcggtttt ttattatccg caaattttat tagcgcccag gtgacagtcc
      361 cggccggacc tgggctggat gtcggcctgg agccaactga agcgattggc ctgccggcga
      421 ctgatattcg gataattatt gcccggatta tcagggtgat tttgggtttt gtcggcatta
      481 tcggggtgat tattgttttg tacggcggct atacctggat gacagccgcc ggcaatgaag
      541 aaaaaatatc acaggccaaa aagatattgc tcaatggcgg tataggtttg attattgttt
      601 tgtcggcgtt cagcattacc agttttatta tcagccggct gcaagccgct ttagtaggtg
      661 aattcgcacc gactccggga gccggtcctt ttgtgggtgg tggtttgggc gttggtatta
      721 ttgaaagtca ttatccggcc cgcaatgcta ccggcattcc ccgcaatacc aaaattattg
      781 tgacctttaa ggaaaaaatg ctgctggagt cgctggtcaa tgacaacggc acgcccgacg
      841 atctgaccga tgattttatc cagccggaat cagtcagaat cagaagaaca gttgatgatc
      901 ggtcagccgg accatttgtc gtcaatgtcc gggcggctta cactgatgat ttgagaagtt
      961 ttgtctttga tccgtttgat ttgctgggaa cagccactga aaatgtttcc tacacggttg
     1021 ccctgaccga ccaggttaaa agatccaatg gtcagtcagc ttttgggggc ctgggcagtt
     1081 atgactggag ctttgaagtc agcactttgg ttgattccga gccgccccag gtggaaagcg
     1141 ttattccagt ggctgattca gtcaatcccc ggaacatcat tattcagatt aattttaatg
     1201 aagcgatgga tccaacgacc gttaccggca gtactggagt tggctttaac aagctggtag
     1261 tttacaacag cactgaccag tcgccggtca gcggcacttt taatgttagc aatcaatata
     1321 aaacagttga gtttatcagc aatgataaat gcggggttaa ttcctgcggg gaagatgttt
     1381 tctgtctgcc gggcgataag gagattttag tgacggttaa agccgcttct ttgtctgaag
     1441 cgccgccaga agccttgttt ccgtataacg gcgtggttga tgttgtcggc aattccctgg
     1501 acggtaacca ggataacgcg gctgacgggc cgccggctga tgattatttc tggcaatttc
     1561 agactaataa cacgattgat ctgacgccgc caattattga atctttggga ccggataaat
     1621 tttcagcggg cgtcgctctg gatgaagtcg tggccgctac tttttccaaa ttaatttcag
     1681 ccagctcgct caatacttcc aatctggttt tggaagggat taattactgg acaggcgccc
     1741 ggaataacat gattgaacaa aagacaactg gcttgatttt tcatgattta ttcagtcagg
     1801 atactcctta taatccgttt gtgacttccg gagtcagaga tatttatcaa aattgttatc
     1861 agccgtgtat tggaccataa aacaaggttg ataaggggat atcaggggtg ataaagggaa
     1921 attaagggaa actattttaa aaaatgttta agaataaaaa caaaattcta ttaattgctt
     1981 ggctgttaat tgtttttagt gtctttttgg tgacttcaaa tgttttggcg caggttgatg
     2041 tcggggttca gcctttggaa gaaatcggtt tgccgaccca ggacatcaga ataaccattg
     2101 ccaatattat cagaatcgcc ctgggctttg tcggcatagt cggggtaatt ttggttttat
     2161 acgggggctt tgtctggatg acttccggcg gcaatgaaga acgggtcagc caggccagaa
     2221 aaattttaac cggcgcggcc attggcttgg taatcgttat ttcagcttat gctatcacca
     2281 gttttattat cagccggctg gctgaagtaa ccggcgcgcc tcaagccggg gttagttccg
     2341 agtttcccgg atttccgccg gggttttccc cgggcgaggc ttttttggtc agaagcgctt
     2401 ccccggttgg cgctgtcccg atcagaaatg tcaaagtcag agttttattc aaccggtcag
     2461 ttgatcaggt ttctgtggcc ggtaatatta cagtggccgc ggcggcggat ggtggcctgg
     2521 tagccggtga ttacaatgtc agcggatcaa cagttgaatt tactccccag gcggcctgtc
     2581 ctgaacctaa tcaaaattta agatgttttg ctgaaaatac tgaatttgtc gttaatataa
     2641 tcagcggggc tgacggggtt aaaagctctg acggccggga gctggtttgc ggtctggccg
     2701 gcagcaactg ccggagcagt tttaccaccg gcagtctgat tgatacccgg ccgccgacag
     2761 ttgatttggt cagtccggtt gccggtcagt cgttaagcgt tgacgatata attttactgg
     2821 aagcattggc tcgggatgat tccgggatag cttatgttga attttacgcg gatgatgtct
     2881 tgattgacac cgccgctccg tctttaagtg tttcgccggc tgactacact gcccaggcca
     2941 actgggacac caccggctta attttaggtt ccgcgcacac tattcaggcg atcgcctttg
     3001 acattgatga tcatcagact gaatcccgga ccatttcagt cactgtccgg gcggctcatt
     3061 gttttgatgg cctgcagaat caggatgaaa ccaatattga ctgcggcggc gctgactgcg
     3121 gctcttgcga cggcgaagcc tgcgtccaga attcagactg cgcctccggc cagtgtgttg
     3181 acggtttttg tttaagactg cctttgatta cccgggtgga tccggctgac ggcgccgtcg
     3241 gcaattatgt ttcaattatt ggccaggctt ttggatcagc ggctggtgaa gtggttttta
     3301 tgggcgatcc ttttaatccg gatgatgatc ggacagcttc cctggctctg tgcgccgccg
     3361 cctggcggga cagccaggta gtcgtgaccg tgccggccgg agcggtcagc ggaccggtta
     3421 gattaaccac agctggtaat ttatttgatc agactaatga tacccgcggc tggcagggtg
     3481 attttaacgt gaatgatact gtccggccgg gtttgtgttc aatttcgccg acccggggtg
     3541 aatacggcgc agtggcgaca attgaaggca ataatttagg cgatcaaaca gccagcctgc
     3601 gttttaaaga aactgaagcc gaaagcattg ccagctggac agatgttaaa attgacggta
     3661 ttgctattcc aaatattaat cctgaagagg tgccggtttt cgcgactgtc aataatctta
     3721 gaagcaatag tctgaatttt acagtccaga cttcgacgac cgcaccgagg attgattacg
     3781 ttgatccggt ggccgggcca gtcggtcagt atctgactat ttttgggact aattttggcg
     3841 gccagaccgg tcaggttttt tttgtcagcc ctgacggcca ggaaattttg gcggacacct
     3901 cttttcctga aaactgcgct gataaatttt ggcacgacag ctatatcaat gtcaaagtgc
     3961 ccaacgtgcc gattggcgat tatcaaataa aggccgtcaa aggaacgcag gaaagtaatt
     4021 tagtggcctt taaagttaat aatgctcccc ggaccccggg tatctgccgg ctgtcgccgg
     4081 acaacgggcc ggttgggatt gaaatgacta tttacggcga gcggtttggt tctgacgcca
     4141 ataaagtccg ctttttcaga ggccaggaaa atggctcaat tattgcctgg gaagaccggc
     4201 aaatcagaat tttggtgccg cccctggccc agactgggcc ggtggaagtg atcgattcca
     4261 gtgggacatt gagtaattca gttaattttg cagttggttt ttgcagttcg acgacctgct
     4321 cggaaactga agattgctgt ggagacggct cctgccggga agccggcagt tgtcaggtcg
     4381 tcccgccctt atgcgcttac agctggactt ttacgaccgg ccggggcgga ctgaatgatc
     4441 cctgccagac tgacgctgat tgtctggccg ggctggcctg tgatcctttg tccaaaacct
     4501 gtcaggaggg tttcccccgg gtagttgagg atcaggaatg tctggaaacg ccccagagcc
     4561 cctcgccctg gaagagcagt accgacgcct gcgttaacgc cggcattgcc gcccggttta
     4621 acatgaacat gaaagatgtt actttgaacc cggccaatat tattctggcc caatgcaaca
     4681 ccggcgccag attccaggct gacgattgcg atcagccggt tggtctgaac cgggtggaaa
     4741 ttaatcaggt gattgatatt gacggcaata ttcaggaggg gtttgtggcc gtgcccggcg
     4801 ccagtttgcg ggctaatacc tggtatcagg tgactttaac gaccggtctt gaaagtgaaa
     4861 ctaaccggtc attatcccag gattatctct ggacttttaa aacacgcgat gacaccactc
     4921 cttgtgaaat cagccatatt caggtttcgc ctcaatacaa gactttaact tataaatttg
     4981 aagaacaagg ttatctggct cagccgacag cggccaactg taataccctg aatcctgaca
     5041 gctacgcctg gaactggagt tcaactgatc cttccagagc ggccgttgtc aataatgtca
     5101 ccagcaaaac ccaggcgacc gctttgaatg aaaccgaatc agatagtccg gttcaggtga
     5161 cagccgccat tccaaccgaa cagaaatcag attttggcga gctgacggtt gattttggcg
     5221 atccggaagt tatcctttac tggcctaact gtcgggcggc ctgcgttaac gcttcaattg
     5281 gcgctaaatt taatgttgat cttcaggcta acactctcaa taataacact ttgcggcttt
     5341 atgcctgcga taatgaaaat tgtactaatt tagatcaact ggcattaaat aatattgttt
     5401 ataatccgct ggatttggaa gtggaattta tgacggccgc cgctttggaa gttaataaat
     5461 tttaccgggc ggtgattacc agcggggtga ccagcaccag caatgttaca ttaaccaatt
     5521 taaattattc aacaaccggt ggagcgctgg acggtttttc ctggattttc aaaactaaac
     5581 ctgacggcag tttatgcgcc gttgatcaga ttaatgttcg gccgggctat aaagaacttt
     5641 atttaattgg tcaaagccag ggctattatt cccaggcgat cggctcgccg gatgaatgca
     5701 atcccggcgg ccagcggctt aatccttata gttataactg ggactgggat tcaacccggt
     5761 caagcgtggc gacagtttcc aacagcgatc tggatccggt tgacggcaga attgatccca
     5821 aacaggccgc ccggtcacgg ggggaaggtg agactgatat tcaggccggc acccagggac
     5881 aggttggtca gggccgttta aaattggtct gcggctttac ggccgatgat cagtgcccgg
     5941 cgccggcgac cattgaaact tatggagttg atgataatag ctgctgcgct ccgcggccaa
     6001 aaattgtcag aaccaggccg attgaccagg cggttagcgt ctgtcgtaac gctgttattg
     6061 aagctgattt tgatcagctg atggagctgg gatcaatttt gggaaatgtc ctggtggagg
     6121 ctaactatgg cgatcagccc tgtccggctg gcggccagag ccggtcaatt tggggccggc
     6181 ttgtccagtt tttcaaaaat attttcagtc gggatattca ggcccaggcg gctgacaact
     6241 ggtgcgccgt ggccggccga accagccttt tccaggaaaa tgatcagact tctctccagt
     6301 attatctgga acagcccctg gagccgggcc gtcagcaccg ggtgacaatt cagggcgatc
     6361 ccaatgtgtc tgatcaaaat aaagaaggag tcttaagttt atcaggtttg acaatgaacg
     6421 gcgattatca gtggagcttt acgaccggcg atgaaatttg tgatattgat tacgctgaag
     6481 tcactatcag cccgccgggc gaggttggcg tttatgatct gttgacctgc gccggccggg
     6541 atgactgtcc gggcgatcag gatagttcaa cgccgggtaa tcagcatttt tacaaagctg
     6601 tcgcccggga tgacagtggc gtgattctgc cggcaaattt tgaatgggtc agaaccggcg
     6661 atcaggtcat taatttatcg gccacgaccg accagcagat tgccgtcacc ccgtcagccg
     6721 ccaacggcaa gcctttggta acagttgttg ccagggggat tccgccgaca cccgggatgg
     6781 cttccaaaac agttaatgtc tttgttttcc tgtgcgaaaa tccctggcca tcgctggcag
     6841 aatttcctta cagagattta acgaccaatt ttagcacttt ttactgccgg gacaggggag
     6901 cggccggttt tagcgatgac tatccgcagt tttcagacac gccggcggtt acgccgactg
     6961 accccagaat tattaaagaa tttttattca tagttgaatg attatgacca gagctgactt
     7021 aattaagaat atttgcaaat ttagtttagg attattgatt atctcagctt ttgttttgac
     7081 tggatctcca gctcaggcgg ccgaagcgat tggcattaga attttggcta atccgggaag
     7141 actgtcgcct tttggctggt attttcaaaa tgtgcctaat ccgggccagc cggagccatt
     7201 attggttaac ggttatccgg ccattcggga aggccggaca gtttatgtcg ccgccaccaa
     7261 tcaggtcggc gacgccttgt acggcaatat ctatttgatt tcatacagtg aagacgccag
     7321 ccagtcaacg attgatattt ttaatcagct gctggagaac tgggatttta atataaatgt
     7381 taacgattta gtcgtcaggg agcagctccg gcgggatatg aagcgggctg gcgatatgag
     7441 cgatgtttcc ggtctgttgg agcgatatcg gcgggtcaac ggcaactatc ctttattgca
     7501 ggcgggcact tatatcagag gattgtcatt cagcgtctgg ccttcctggc aggcgacttt
     7561 ggctaatctg ctggccagcg ccttgccgca ggacccgctg aacagattaa tcggctgtca
     7621 ggatccttat gatcagacaa cctgctggga tgaagtcaat cagcagttcc agtgcagtcc
     7681 cgagtccaga acttacgcgt atcaggttga tgataacggc gatcaggcta gtttatacac
     7741 caactatgag tacactggtc caggctcctg gcggacaggc gaatacggtt tattaaccag
     7801 tcagttttgt tttaccaatg aaagcgcttt tttgaatgac gaagatggtg acggaatttt
     7861 aaacagcgct gacaactgcc cgactgttta taatcctgat caggctgatt ttgaccggga
     7921 tggtttgggc gctttatgcg atctgtgcga ccttgatcct tccaacgata ttgaccggga
     7981 ttttgtttgc ggcggcgttg acaactgtcc caaaaatttt aatcctgatc agacagacgc
     8041 cgacgccgac ggaattggtg acgcctgcga tattatggtt tgcggcaacg ccattaaaga
     8101 aggcgatgag gtttgtgatg ggttaggcag tattccggct aactctacct gcttaaacga
     8161 ttgttccggc tggacttgta atgaaggtta ttcccggttt gataatatct gcgatattga
     8221 tacagacggt gatttggtcg ctgacagcct ggataactgc tccaatcttt ataatcccgg
     8281 ccagctgaat agtgacaatg atatttacgg tgacgcctgc gactggtgtc cggacgattc
     8341 tgacaacgat attgacgggg acggctactg cgccggtccc agatacagcg gcccgcgcct
     8401 gggagcaaat gatatttgtc cgaccgtggc tgatccggag caggccaaca cggacggtga
     8461 tgagctgggg gatgcctgcg atcagtgtcc ggatgatccg gctaatgatc cggatgatga
     8521 cggtgtttgt gccggcgcca gatttaacag tcctaaacta gcgggtagtg ataattgccc
     8581 ggccgtggct aatcaggatc aggctgattt tgaccgggat ggcattggcg acgcttgcga
     8641 tcctcaaacc tgcggcaata atattattga agctggcgag aaatgtgatc aaaatcaggg
     8701 cttggctggc attatctgta ctggtcagga ggaactgtat tgttcggtcg attgtcagat
     8761 tagctgtacc ggccagggcc agccggttta cgccatctcc ggcagtgcct ggagttccaa
     8821 catcggctgg attaatttaa gccccagcaa cggcggggtg accattagtt tgaccaacac
     8881 ctttgtcggt tccgcctgga gcgccaatgt cggctggatt aatatgtcag gcgccacggt
     8941 taatcttgac ggaactgttc atggcgatat tttgggtaat gactacgtcg gcgtcattaa
     9001 ttacgacccg tcacggagcg gcgtcagaat caattcggct gacgggactt ttagcgggtt
     9061 tgcctgggga gaaaacatcg gctggataga ttttaatgga gtttctacca gttggaaacc
     9121 ctgacaagca ggggatatta gggattatta tggtagatga gggtttatta agggtaaaca
     9181 taatcaaccc tcatcaactc ccgatcccgc caaaggcggg atcgggactt catcaacctt
     9241 catcaacctt tatttaaccc ttaatttcaa attttatgcc cgaaggacaa ccaacgccaa
     9301 cagcgccaac aattccacct accccgccta agccgattcc gcccagggag ccggaaccaa
     9361 cgccatcaat cgtcccaccc ccggtgggaa ttgaagatat tttatccggg actgaagcgg
     9421 ctggtccagc cggtccaggc agtccaactc cgccgacaag tgatcagggg caacccccgg
     9481 acattattcc ggaatatcca gaaacaagtt tttttctgaa atatcgtttg ttgataataa
     9541 ttggagtttt agttattcta tttgtggcaa ttgctctgat tattttatgg gtaaccaccc
     9601 gccaaccagt atttcagcag ccagtcaaca tcaacgccat tgatcagccg ccagttaccc
     9661 cgccgcccgc cgtccccgtt gacagtgatc gggacggact aactgatgaa gaggagaaag
     9721 agttggggac tgatctcaat aatccagata ctgacgggga tggtttgact gattttgaag
     9781 aagtcagggt ttataagact gatcctttga atccggatac cgacggcgat ggttttctgg
     9841 atggtgatga agtccaggcc ggatttgatc cgactcaggg cggtggcgcc agattgctgg
     9901 acctggaaag agaaattaat aatattcgtt aattaaaaaa tatgtttggc cgaaacaaaa
     9961 aagaaaaatc catagaatca tcagctgatc agccggctgg tccggccccc ggtaatatta
    10021 gaaccattcc tgaccagttt ttatataaag aagaaaagaa aaaaaccggc ggtttggtga
    10081 ttattatcat tggcgcttta gttttaattg ttttggtggc tggcggtgtc tggctgctga
    10141 caggaagctt tggtcagccg actgaagtca cccgggtgga gaatgttaat ttggaagtta
    10201 aaccgccagt taatgaaaat cttaatgtca accttaatgt taatgccaat ctcaactcga
    10261 atgttaatgc caacctgaac gccaatgcta acgttaatgc cggtttgccg ccggtcatcc
    10321 ccgggcttct ggccagcagt cttgacagtg atcaggacgg cctgactgat gttgaggaag
    10381 aactttatgg aactaatccg aatctgcccg attctgacgg tgacggtcac attgacgctc
    10441 tggagctgat gaacctgttt aatccggctg agccggcgcc ggctgaactg cctggatcaa
    10501 acctggttga gctttatagc aacccgtctt ttgattaccg aatattgtat cccagccgat
    10561 ggatcgcccg gccgattgac actgaactga aagaagttat tttttcttct gaaaccggcg
    10621 aatttattga agtcctggtt tcggaaaatc ccgaggggct gtcggttctc aactggtatt
    10681 tacagcagtc gccgactgtc acgcccagcc aggttcagtc agttgtcacc aaaaacaata
    10741 tcagaggcat tcagagcgtt gacggcctga cagtttatct ggttaagccg gttgattcat
    10801 caatcaccgg cgaggccgaa gccaaacaat atatttacgc cattagttac aatcctggca
    10861 ccaggccgga gcttaatttc aaaagcactc taaagatgat gtacaattct tttgaattgg
    10921 gtcagctggg acagccggct ccgagcagtc cataaagacg tatgatttta ctggcggtta
    10981 atcaggatta tttaaaggaa aagaaactgg ccaaggcctg gtttgaggaa atcacccagg
    11041 cgacctccgg ctgtttgaat ttgaaaaaaa actattttgt ttcgctggct tttattgatc
    11101 agaagaaaat taaaatgcta aacaataaat atcggcttaa aaatcaggta accgatgttt
    11161 tggccttcag ctatcaggaa actgatcagg aagcgcctga caggacatat ttaggcgaaa
    11221 tatttatttg ttatccccag gctgaaaagc aggctctgga gcatcggcag ccggttgagg
    11281 aagaaataaa aactttactt attcatggtc tgcttcattt gttcggccat gatcacgcga
    11341 cagaagctga tcaccgaaag atgaaaaagc tggaagataa aatcaggaaa attaatttaa
    11401 attaaaatgg acaatcaaaa gctgcgtaaa agttttaaat acgcctggcg ggggctggct
    11461 gatattttaa aaaccgaaca aaattttcag atagagctgg ccagcggcct gatggttgtc
    11521 gttttgatgt tttatttcca ggttagcctg ctggaggcgg ctattttaat tttaaccatt
    11581 ctgactgttt tagtttttga aatagttaac acggtcattg aacggctggt tgatttgatc
    11641 aagccccaaa ttcatcagca ggccggacga atcaaagaca tgaccgcggc tatcgttctg
    11701 gtggcttcaa ttggcgccgc gattattggg ctgattattt ttctgcccta ctttttttaa
    11761 gaagcgccct ttgaaacatt tttttgttca gaaaataaac agtttcgctt tgcaattttt
    11821 tcaattgtgt aatagactta agccatcagc cactagtcaa cagccatcag gatacgaatt
    11881 tttaaatttt tcaacccgtt gactgctgat tgttggttga ctgttaactt aaataaatgc
    11941 gagacgaaat tatcaccggc cttgacgttg gttcaacaac tatccggatt gttgtcggtc
    12001 aaaaaatagt tgatagcaat aatgaagaaa aacttcatat tattggggtg gctgaaactt
    12061 cggccgaggg gattagtaaa ggggtaattg tcagcattga cgacgccgtt tcgtctattt
    12121 ctagttgttt ggaaaaggcc gaaagaatgg ccggcgtgcc gattgagcag gcctatgttg
    12181 gcatttccgg ctcgcatatt ctctcccagg gatccaaggg agtaattgcc gtttccaaag
    12241 ccaatggtga aatcaatgaa gatgatattg agcgggtcat tgaagccgcc caggccgtgg
    12301 ctacgccgcc taattatgaa atcctccatg tcattcccag aaattttacg gttgataacc
    12361 agatggggat taaagatccg ctgggcatga caggcgtcag gctggaagtt gaagcccaga
    12421 ttattcaggg tttgtcgtct caggttaaaa acttaaccaa aactatttat cggaccggag
    12481 ttgatattga agacctggtt ttagcggttt tggcggccgc tgaaagcgtt ttaagcaagc
    12541 gacaaaagga gctgggcgtg gctttggtca atattggcgg gtcaaccacc agcctggttg
    12601 ttctggaaga aggcgatgtt ttgcacactt caatcctgcc gattggcggc ggacacatta
    12661 cttctgacgt cgccattggt ttaagaactt ctattgatgt cgctgaaaaa gttaagattg
    12721 agtacggctc ggccgtgccg aataatatca ataaaaagga tgaaattaat ttggcggaat
    12781 tcagctccgg agacgaatca actgtttcca gaaaacatgt cgccgagata attgaagcca
    12841 ggctggaaga aatttttgaa ctggttgacc gtgacctgaa aaagattaac cggagcggtt
    12901 tattgccggc cggagtagtc ttaaccggtg gcggcgcccg gctggagggc atagttgagg
    12961 ttgccaagaa aaaattcaga ctgccggctt ctattggcta tccgactgaa atggccagcg
    13021 ccattgaaaa agttaatgac cccatctttt cagccgcttt agggctggtc atttggggca
    13081 gtcagct
//
LOCUS       DASWHZ010000151         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2156147, whole genome shotgun sequence.
ACCESSION   DASWHZ010000151 DASWHZ010000000
VERSION     DASWHZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156147"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGA49_03075"
     CDS             complement(<1..230)
                     /locus_tag="VGA49_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664784.1"
                     /translation="MIPDDRILTFWPLTILIGPEGGRGLAEYEAELLILRGELRGKIV
                     LDLGCGCTEKFAREIHQAGINCQVISVSPDYI"
     gene            complement(444..517)
                     /locus_tag="VGA49_03080"
     tRNA            complement(444..517)
                     /locus_tag="VGA49_03080"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Ala,seq:cgc)
     gene            complement(611..>1759)
                     /locus_tag="VGA49_03085"
     CDS             complement(611..>1759)
                     /locus_tag="VGA49_03085"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX9664785.1"
                     /translation="LFEPNYEGKGQPDSLVVFVPEWPEIKILADVLNQTTYVLGSDYF
                     GECKKANLRMAMYLIKQQDGLGLHAGSKLIRIKDKNNSLTEKGIILFGLSGTGKTTLT
                     IHNHYLTGPERVFILQDDVILMDKQAACYGTENNYYIKTEGLEPSQEILYTAAIQPEA
                     VMENVGVDPASGKVDFTNYDLTTNGREIVLRKDIPLCTDIVDLAKANIIIFITRRNDI
                     VHPVAKLNPVQGAAFFMLGESIETSAGDPTKAGQSKREVGFNPFIIGSKGQEGNRFLE
                     ILKNNPDINCYLLNTGVVGNTDKISIKDSVSMLTEIARNNIDWQANDDWGYQVPVEVA
                     GVDSKKLNPANYFAKEEYNKLVDNLKQERLNWLNQFEDLDPGIKKVFS"
BASE COUNT          473 a          431 c          342 g          513 t
ORIGIN      
        1 aaaatatagt cagggctgac ggagattacc tggcagttga tgccagcctg atggatttct
       61 ctggcaaatt tttcagtaca gccgcaaccc agatcaagaa ctattttgcc ccgaagctct
      121 ccccttaaaa tgagcagctc tgcttcatat tccgccagac cccggccccc ttcaggtccg
      181 attaaaattg ttaggggcca gaaagttaga attctatcgt caggaatcat ggccctcctt
      241 tccttgcgaa accgtttcaa gagtagcttt tttgaaaaat actgtcaatt tgaacttttt
      301 ttacaaataa aaaaatagac tcggcgggag ttgcccgcct gaggcgggtc cgcctcaggc
      361 ggaaacccac ctatcctttt taaattatta tatctggacc taggcagaat cgaactgccg
      421 tctccacaat gtcccgatta ctttggtgga cccaccggga attgaacccg gacctcggca
      481 atgcgaatgc cgtgtaatac cgttttacta tgggcccacg ggtccgccaa agtaatcgag
      541 gatcctcgat cccgccaaag gcgggatcgg gacgaatgca gcgttctacc actgaactac
      601 gagcccaaat ttagctaaat acttttttta ttcccggatc caaatcttca aactggttca
      661 gccagttaag gcgttcttgt tttaaattat caaccagctt attatattct tctttggcaa
      721 aataattggc tggatttaat tttttgctgt caactccggc gacttcaacc ggaacctgat
      781 aaccccagtc gtcattagcc tgccagtcaa tattattccg ggcgatttca gtcagcatgg
      841 aaacagaatc tttaattgaa attttgtcag tgttgccgac cacgccggtg tttaacaggt
      901 agcagttaat atccgggtta ttttttaata tctccaaaaa acgattgcct tcctgccctt
      961 tagagccaat aataaaagga ttaaagccaa cttcccgttt actttggccg gccttggttg
     1021 gatcaccggc cgaagtttca attgattcgc ccagcataaa gaaagccgcg ccctgaaccg
     1081 gatttaattt agccaccgga tggacgatat catttcttcg ggtgataaaa ataatgatgt
     1141 tagctttagc caggtcaaca atatcagtac agagaggaat gtcttttctc aaaactattt
     1201 cccggccgtt ggtcgttaaa tcatagttgg taaaatcaac cttgcccgat gccggatcaa
     1261 cccccacatt ttccattacc gcctctggct gaatagccgc ggtgtacagg atttcctggc
     1321 tgggctccag accctcggtc ttaatataat aattattttc ggtaccgtaa caggcggctt
     1381 gtttatccat taaaattaca tcatcctgca aaataaaaac cctttccgga ccagtcagat
     1441 aatgattgtg aatagtcagc gttgttttcc cggtgccgct taagccgaat aaaataatcc
     1501 ctttttcagt taaactatta tttttgtctt ttatcctgat taatttactg ccggcgtgca
     1561 gtcccagtcc gtcctgttgc ttgattaaat acatcgccat tcttaaattg gcttttttac
     1621 actcgccaaa atagtcagag cccaaaacat aagtcgtttg attgagaaca tcagccaaaa
     1681 ttttaatttc cggccactct ggcacaaaaa ccactaaact gtccggctga ccttttcctt
     1741 cataatttgg ttcaaacaa
//
LOCUS       DASWHZ010000152         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3591707, whole genome shotgun sequence.
ACCESSION   DASWHZ010000152 DASWHZ010000000
VERSION     DASWHZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3591707"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /gene="murJ"
                     /locus_tag="VGA49_03090"
     CDS             complement(<1..1616)
                     /gene="murJ"
                     /locus_tag="VGA49_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9664786.1"
                     /translation="MVKKFKLLINGPSRTITSAAAVIALTGFVSRLLGVVRDRVLAGE
                     FGAGNILDAYYAAFRIPDLVFNLLVMGALSAGFIPVFAGYLNKSNDKQEAWDLASGLL
                     NILIILLIIICGALVLLAPFLVPLIAPGFNQEKIKMTVELTRIMFLSPIFLGLSSLFG
                     GILQTFKRFLVFSLAPIFYNIGIIIGAVVFVRWWGIIGLAVGVVFGALMHMLIQIPTA
                     VNLGFRYKTYLSLTHPGIRKIGRLMVPRTLGLAITQINLVIITIIASILPEGSLAIFN
                     LANNLQSFPVGIFGISYALAAFPTLSEYFVQNKSEEFVQTFSKTVRQILFLIIPTSVL
                     FITLRAQIVRVILGSGQFDWNATILTAAALGFFSLSMFAQALIPLLARSFYARHNTKT
                     PFWAAFIGMIVNTGLALLLAKPLGIIGLALAFTIASIVNLLALYIWLRHKIGRLNEKV
                     IIKSTSKVLVASLIMAGLVQLTKYLIALTLDIDTFIGIFSQGLVAGLVGIVVFIAVSY
                     LLKSDEIKFFETVFKRRVLGIFRTEYKIKEG"
     gene            complement(1741..2052)
                     /locus_tag="VGA49_03095"
     CDS             complement(1741..2052)
                     /locus_tag="VGA49_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX9664787.1"
                     /translation="MVKKYFYKNINDQEKALFQDYFETKIKQLHKYLKRYDDNELKLS
                     AKAEKFATKHAYKVTFTLALPANQLMAWEDDHTISEAVDLAYDKLVIQLSKLHKRLTK
                     K"
     gene            complement(2074..>3172)
                     /locus_tag="VGA49_03100"
     CDS             complement(2074..>3172)
                     /locus_tag="VGA49_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664788.1"
                     /translation="VVRKNVPLQGTGLSEKELDIIYYVNVYRALLKYDPILVNYLLFK
                     LSIPNWSKASKTDIDWTCQNFLKIRKTIDKLLTNPNSERLHRLFKQYTIIFLILRDII
                     DDDPSTAGQIFSDPQKLNDSVREAVNHRYKMAQGKLRRSLVRSIIYIFITKMLLALIL
                     EFPVDIFIEKKINYTSLITNITFPPLLMLLVGASIRVPSTKNTDRIVRLINEIVYEES
                     NEGLYQKVSKAKAGGFLSRQLFRIVYLIMYVVTFGLIIYLLRLVNFNAVNIVIFLLFL
                     SVVSFFGFRIRQSARELKVLPKRESFLTLMVDFFSIPILKAGRWLSLNLSRINIFIFI
                     MDIIIEAPFKTLIEIFEDWLSFIREKKEEIT"
BASE COUNT         1050 a          666 c          600 g          856 t
ORIGIN      
        1 ccctctttaa ttttatattc tgtcctgaaa ataccaagga ctcttctttt aaagacagtt
       61 tcaaaaaact taatttcatc agatttaagc aaatagctga ccgcgataaa gacaacaata
      121 ccgaccaggc cggcaaccaa accctgcgaa aaaattccga tgaaagtatc tatatcaagc
      181 gtcaaagcta ttaaatattt agttaactgg accaggccgg ccataattag cgaagcaacc
      241 agtacttttg aagttgattt tataataact ttttcattca gccggccgat tttatgtctt
      301 aaccagatat atagagccag tagatttact atgctggcta tggtaaaagc taaagccaag
      361 ccaattattc ctaagggctt agctaacagt aaagctaaac cagtattaac aatcattcca
      421 ataaaagccg cccaaaacgg cgtcttggta ttatgcctgg cgtaaaatga ccgggccagc
      481 aaaggaatta aagcctgggc aaacatactt aaactaaaaa agcccagagc ggcggcggtt
      541 agaatggtgg cgttccagtc aaactggccg ctgcccaaaa tcacccggac aatctgggct
      601 cttaaagtaa taaacaaaac gctggtcgga atgattaaaa ataaaatctg ccggactgtt
      661 ttagaaaagg tctgaacgaa ttcttcggat ttattttgaa caaaatattc agacaaagtt
      721 ggaaaggccg ctaaggcgta tgaaattcca aaaattccaa ctggaaaact ttgtaaatta
      781 ttggccaggt taaaaattgc cagactgcct tcgggcagga ttgaagcaat gattgttata
      841 ataaccagat tgatttgagt aatggctaaa cccaatgttc ggggaaccat cagccggccg
      901 atttttctga tgcccgggtg agttaagctt aaataagttt tatagcgaaa ccccagatta
      961 acagctgttg gaatctgaat taacatatgc atcagggctc caaaaaccac gccaacagcc
     1021 aaaccaataa tcccccacca cctgacaaag accaccgccc cgataattat cccaatattg
     1081 taaaaaatcg gcgccagtga aaaaactaaa aatcttttaa atgtctgtaa aatgccgcca
     1141 aataaactgc ttaatcccag gaatatgggg cttaaaaaca ttattctggt cagctcaaca
     1201 gtcattttta ttttttcctg attaaaacca ggggcaatca aaggcaccaa aaaaggagcc
     1261 agtaaaacca gggctccgca aataataatc aaaagaataa ttaatatatt taaaagaccg
     1321 ctggccaggt cccaggcctc ctgtttatca ttgcttttat ttaaataacc ggcaaaaacc
     1381 ggaataaaac cggctgacag agctcccatg accagcaaat taaaaactaa atcaggaatt
     1441 ctaaaagcgg cgtaataagc gtccagaata tttccggcgc caaattctcc ggccagcacc
     1501 cggtccctga ccactcccaa cagcctggaa acaaaaccag tcagagcaat aacggcggcc
     1561 gctgaagtaa ttgtccggct gggaccattg attaataact taaatttctt gaccataata
     1621 taaaagtcac tagtcactag gagtctaatc ccgaacccaa tgactaatga ctaatggcta
     1681 gtgactgccc gcccgccgac ttgtccgcca aagccttggc gtaggcgggc tgactaaaat
     1741 ttatttcttc gttaaacgct tatgcagttt agacagctga atgactaatt tatcgtaagc
     1801 caggtcaacc gcctcggaaa tcgtgtggtc atcctcccag gccatcagct gattggccgg
     1861 gagagccaga gtaaacgtaa ctttgtaagc gtgcttggtg gcgaattttt ccgccttggc
     1921 gcttaattta agttcattat catcatatct tttcagatat ttatgcaact gtttaatttt
     1981 agtctcaaaa taatcctgaa aaagagcttt ttcctgatca tttatattct tataaaaata
     2041 tttctttacc atatgtttct aggttatagg ttattaggtt atttcctctt ttttctccct
     2101 gataaacgac agccagtctt caaaaatttc aatcagagtt ttaaaaggag cttcaattat
     2161 tatatccatt ataaaaataa aaatattaat ccgcgataaa ttaagagaca accagcggcc
     2221 ggctttcaaa atcggaatag agaagaaatc aaccattaaa gtcaggaaac tttcccgctt
     2281 aggtaaaact ttcagttcgc gggctgactg cctgattcta aaaccaaaaa aactgacgac
     2341 tgacagaaaa agcaggaaaa tgacaatgtt aaccgcgttg aaattaacca gccttaacaa
     2401 ataaattatc aggccaaagg tcaccacgta cattatcaga taaacaatcc ggaatagctg
     2461 gcgcgataaa aatccgccgg ctttggcttt actgactttt tgatagaggc cttcattgga
     2521 ctcttcgtaa actatttcat taattagccg gacaatccgg tcggtgtttt ttgttgaagg
     2581 caccctgata gacgcgccga ctaaaagcat cagtaacggc ggaaaagtaa tgttggtaat
     2641 taaagatgtg taatttatct ttttttcaat gaaaatgtca accgggaatt ccaggatcag
     2701 ggccaaaagc atcttggtga taaagatata aataatgctc ctgaccaggc tgcgccgaag
     2761 cttgccctga gccattttat agcgatggtt aacggcttcg cggacactat cgttcagttt
     2821 ttgaggatcg ctgaaaattt gcccggccgt ggacggatca tcgtcaataa tatcccgcag
     2881 aattaaaaaa attattgtgt attgtttaaa taaacgatga agtcgttcag aatttgggtt
     2941 agtcagcagt ttatcaattg tttttctgat ttttaaaaaa ttctgacagg tccagtcaat
     3001 atcagtttta gacgctttgg accagttggg gatgcttaac ttaaacagaa gataattgac
     3061 taaaattgga tcatatttta acaaagcccg gtaaacatta acataataaa taatatccag
     3121 ttccttttcc gacaaaccag tgccctgaag tgggacgttt tttctgacaa cg
//
LOCUS       DASWHZ010000153         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3255010, whole genome shotgun sequence.
ACCESSION   DASWHZ010000153 DASWHZ010000000
VERSION     DASWHZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255010"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VGA49_03105"
     CDS             <1..1084
                     /locus_tag="VGA49_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9664789.1"
                     /translation="SIYLNHFRELTYLKSIGDGVIAIDRHWIVTRWNKAAAIITGWSE
                     VEAVGQPLRNVFKIIKESSRRENISFIENAIVMGETMFMANNSLLIRKDKTEIPVGDS
                     AAPIFSANNAITGAIIVFRDMSKEREVRLLQSDFAYASHQLRTPVNKALWYTEVLMED
                     VRAKPILQELGIAHLSMQSTAKIIGKLMTISDIDRGNIILKFSQANLNKIIQEIKISL
                     KQELTEKKIAFKAINQSEYRIIKTDPKKLKAALWEIMNNAVRYNKPDGKIDLRISSNK
                     QGILFTVSDTGIGISIKEQPLIFTKFFRGGNIDVTKIIGAGLGLYIAREYIKLLGGKI
                     WFESKKNKGTTFYLLMPLENNRVKKS"
BASE COUNT          464 a          323 c          312 g          415 t
ORIGIN      
        1 aagtatttat ttgaaccatt tccgggaact gacttatctt aaaagcattg gcgacggcgt
       61 cattgccatt gaccgccact ggattgtcac ccgctggaac aaagcggccg ccattatcac
      121 cggctggtcg gaagtagagg cggtcggcca gcccttgaga aatgttttta aaatcattaa
      181 ggaatccagc cgccgggaaa acatcagttt tattgagaac gcgattgtaa tgggtgaaac
      241 catgttcatg gcaaacaatt ctctgctgat cagaaaggat aaaactgaaa ttccggtggg
      301 cgacagcgcc gctccaattt tttccgccaa taacgcgatt accggggcga taattgtttt
      361 cagggatatg tccaaagaaa gagaagttcg gctgcttcag tcagattttg cttacgcctc
      421 ccatcagctc cggacgccag tcaataaagc tctctggtac actgaagttt tgatggaaga
      481 cgtcagggct aaaccaatct tacaagaact gggaatcgcc cacctttcaa tgcaaagtac
      541 cgccaaaata atcggtaaat taatgacaat ttcagatatt gaccggggca atataatttt
      601 gaaattcagc caggctaatt taaataaaat tattcaggaa ataaaaatca gcctgaaaca
      661 ggaactaacc gaaaaaaaga ttgcttttaa agcaattaat cagtcagagt acagaatcat
      721 taaaaccgat cctaaaaaat taaaagccgc cctctgggaa atcatgaata acgccgtcag
      781 atacaataaa ccggacggta aaattgatct gcgtatcagc tccaataaac aaggtatttt
      841 attcaccgtc agcgataccg gcatcggcat ttccatcaaa gaacagccgt tgattttcac
      901 caaattcttc cggggcggta acattgatgt taccaaaata atcggcgccg ggctgggttt
      961 atatattgcc cgggaatata ttaaactgct gggcggcaaa atttggtttg agtcaaagaa
     1021 aaacaaaggg acgacttttt atcttttaat gcctctggaa aataatcggg ttaagaaatc
     1081 ttgaaatttt aaatatcttg ctttaaaata aaaccacccc aacctatgca ttatttatcg
     1141 gttggggtct ttttatatat acaaatttat ttattctggt gggcgtggtt tggaacgaga
     1201 cgagacttat cactctatgt ctcgggggtt cgattcccct cacgtccatc ggatacaaat
     1261 caatttgaca tatctcttat actgtgttat tctactcata agagtgataa gtcttgttaa
     1321 tcgtaccaaa cgagacaata gtaaaacccc gctggccaag gccggggggt tttatagttt
     1381 ttgttaagaa aacccatctg tgaattttct ttaatccagc taagggttaa cctggacgtc
     1441 ggcctccgtt gaaccattaa cgtttagtcc attagcgccg gccccggtct cgactgaccc
     1501 ttcaacttca attt
//
LOCUS       DASWHZ010000154         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3255400, whole genome shotgun sequence.
ACCESSION   DASWHZ010000154 DASWHZ010000000
VERSION     DASWHZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255400"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VGA49_03110"
     CDS             complement(<1..692)
                     /locus_tag="VGA49_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX9664790.1"
                     /translation="MLTAIYTTILYQPLYNILIFLYNIIPGQDVGIAIIALTVLIKLL
                     LYPLSRKSIKSQKDLQELQPKIDQLKEQYKDDREKMAGEMMKLYKESKVSPFSSCLPL
                     LIQLPFFIAVYQVFRSGLTSESFDLLYSFVANPNQINHIFLGLIDLSRPNVVLALLAG
                     AGQFFQTKMYTRRKPEQKSTGSKDENMMAMMNKQMLYVMPIFTVVIGFSLPGGLTLYW
                     LVTTLLMIFQQY"
     gene            complement(752..1063)
                     /gene="yidD"
                     /locus_tag="VGA49_03115"
     CDS             complement(752..1063)
                     /gene="yidD"
                     /locus_tag="VGA49_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9664791.1"
                     /translation="MKKLKRPCLIYCEKRGHYRAMSFFTLQIRNLVLNSIKIYQKTLS
                     FDHGVFSGLFPNGFCRYYPSCSEYTYQAIVKYGVLKGGLKGAWRLIRCNPLSKGGHDA
                     I"
     gene            complement(1008..1346)
                     /gene="rnpA"
                     /locus_tag="VGA49_03120"
     CDS             complement(1008..1346)
                     /gene="rnpA"
                     /locus_tag="VGA49_03120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9664792.1"
                     /translation="MLPKQHRLSKDRDFQKVFKAGRSVFSAILNLKVLKTSNQVSRFG
                     IIVSNKVSKRATARNKIKRQLRGALQTNLEKLKPGYDCVIIAKQTILDKEYEEIEATL
                     FDLLRKAGTL"
     gene            complement(1459..1596)
                     /gene="rpmH"
                     /locus_tag="VGA49_03125"
     CDS             complement(1459..1596)
                     /gene="rpmH"
                     /locus_tag="VGA49_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9664793.1"
                     /translation="MPKRTYQPKKRKRKRVHGFKARMSSPGGKRVIKRRRAKGRKRLT
                     H"
     gene            1797..>2161
                     /locus_tag="VGA49_03130"
     CDS             1797..>2161
                     /locus_tag="VGA49_03130"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX9664794.1"
                     /translation="MTNEQLWQAVLGELELSLSKANFTTWFKSTSISQKEAGKIIIAV
                     PNAFTRTWLEKKYNDSILKALKSLTDNEIKEVFYKVETLPQKQPAYSPSPKINGQGSE
                     SIDNLVDVFGLNKKYVFEN"
BASE COUNT          606 a          473 c          446 g          636 t
ORIGIN      
        1 aagtattgct gaaaaatcat cagtaaagtc gtcaccagcc agtacaaagt cagcccgccc
       61 ggcagactaa agccaatcac gactgtaaaa atcggcatca catacagcat ctgcttgttc
      121 atcatggcca tcatgttttc gtctttgctg ccggttgatt tttgctccgg cttccggcgg
      181 gtatacattt tggtctggaa aaactggcca gcgccggcca acagggccag gacgacattc
      241 ggccggctca aatcaatcag gcccaaaaaa atatggttga tctggttggg attggccaca
      301 aaagagtaaa gcaagtcaaa gctttccgaa gtcaggccac tgcgaaaaac ctgataaacg
      361 gcgatgaaaa aaggcagttg aattaacaac ggcagacagg aagaaaacgg actgactttt
      421 gattctttat aaagcttcat catttctccg gccatttttt cacggtcgtc tttgtactgt
      481 tctttcagct gatctatttt tggctggagc tcctgtaaat ctttttggga tttgattgat
      541 tttctggaaa gcggatagag caaaagttta atcaaaactg tcagcgctat aatggcgatg
      601 ccaacatcct ggccgggaat aatattatat aaaaagatca ggatgttata gagcggctga
      661 tatagaattg tggtgtagat tgctgtaagc ataatgtaaa ggcgctagtc attagtcaaa
      721 accctggtgg ctggtgacta gtgactaatg gctatattgc gtcgtggcct cctttggaca
      781 ggggattaca gcgaatcaac cgccaggcgc cctttaggcc gccctttaaa acaccatatt
      841 tgacgatcgc ctggtaagta tactccgaac aggacggata ataccggcaa aatccattgg
      901 gaaataagcc tgaaaaaacc ccgtgatcaa aagacaaggt tttttggtaa attttaatac
      961 tatttaagac taaatttctg atttgaaggg taaaaaaaga catagctcta tagtgtcccc
     1021 gcttttcgca ataaatcaaa cagggtcgct tcaatttctt catactcctt atccaatatc
     1081 gtctgtttgg caataattac gcagtcataa cccggcttca atttttccaa atttgtctga
     1141 agcgctccgc gaagctgtcg ttttatttta tttctggcgg tcgctctttt ggaaacttta
     1201 ttggaaacaa ttattccaaa ccggctgacc tgattgctgg tttttaaaac ttttagattc
     1261 aggattgcgg aaaaaacaga ccgccccgcc ttaaaaactt tctgaaaatc tctgtctttg
     1321 gaaaggcgat gctgtttggg aagcatttta tttcagttaa acagtcaacg gtcaacagcc
     1381 agcggggaaa atttttgcat taaaggctcc ctgttgtcag ttggctgtta actgttggct
     1441 gttggctgct tgctatattt agtgcgttaa tctcttccgg cctttggctc gtcggcgctt
     1501 aataacccgc tttcctcccg gactgctcat ccgagcctta aagccatgga ctctttttcg
     1561 tttacgcttt ttgggttgat aggttctttt aggcataaat ttaactttag ttcttcagtt
     1621 ttcaattaca ataagtatat aaattttaac cccgcctgtc aatcacggac ggcttgtcag
     1681 ctgaggtcgt ttatattatc ctattcatac acagttattc aacatcttgt taacaaatag
     1741 tccggactag ccgccttttg accgctgacc ttttgtgata tactctattt aaatttatga
     1801 ccaacgaaca actttggcag gccgttcttg gcgagctgga actttcctta agcaaggcca
     1861 acttcacgac ctggtttaaa agcacttcca tctcccagaa agaggccggc aaaatcatta
     1921 ttgccgtgcc caatgctttt acccggacct ggctggaaaa aaaatataac gactccattc
     1981 tcaaggcttt aaaatctcta actgacaatg agatcaaaga agttttttat aaagttgaaa
     2041 ccctgcccca aaagcagccg gcttattcgc cgtcgcccaa aattaacggc cagggatctg
     2101 aatcaatcga caacctggtt gacgtattcg gccttaataa aaaatatgtt tttgaaaatt
     2161 a
//
LOCUS       DASWHZ010000155         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2164908, whole genome shotgun sequence.
ACCESSION   DASWHZ010000155 DASWHZ010000000
VERSION     DASWHZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164908"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="lysS"
                     /locus_tag="VGA49_03135"
     CDS             <1..1173
                     /gene="lysS"
                     /locus_tag="VGA49_03135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9664795.1"
                     /translation="LFKTKAGELTVQAKKFRLLSKALLPLPEKWHGLKDIEERYRKRY
                     LDLIMNPEVKERFVIRSKIVESIRRLLLNKGYLEVETPVLQTIYGGGFAKPFITHHNA
                     LDIPLYLRISDELYLKRLLVGHFEKIFEFCRDFRNEGLDPSHNPEFTMLEAMTAYQDY
                     RFSMDLVEEIYETAAKEVLGTTKINYQEHQIDLKRPWKRLTLHQAVKEFTGHDLVKVS
                     DLSEAIEIAAGLKIDKRKIKPLQSVGEITNLIFEEKVESNLIQPVIIYDYPVEVSPLA
                     KKSRTNPKVVERFEHFIFGKEHGNHYSELNDPVELKKRFIEEKNKKEAGFEEAHQTDL
                     DFVEALEHGMPPATGIGIGIDRLAMLFTNSRSIKEVIFFPTMKPKTKSIEENGKAK"
     gene            1170..1583
                     /locus_tag="VGA49_03140"
     CDS             1170..1583
                     /locus_tag="VGA49_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX9664796.1"
                     /translation="MKKIMVFGTFDIIHPGHLNFFEQAKKQGDYLIVVVARDTNVRKV
                     KSLSPQNNEQVRLKQLKKIMIIDRAVPGNIDDPYKVIIENKPDIICLGYDQKTFVDGL
                     LAKIKQGQIKDIKIVRLNSFKPEKYKSSKLRHNYA"
     gene            1576..2853
                     /gene="serS"
                     /locus_tag="VGA49_03145"
     CDS             1576..2853
                     /gene="serS"
                     /locus_tag="VGA49_03145"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9664797.1"
                     /translation="MLDIKFIRDNQKLVQENAGNKGIKIDIKKLLKLDDQRRKLITKI
                     EKLNSQKNQANQEIAKAKGADKAKKIKMMKKVADKISQLNPELSQVEKDYYDQMSLVP
                     QIVDKKTPVDPEGKKSKIERRVGNVPHFNFKQKDHLELGRLLDIIDLERGVRVMGTRG
                     YFLKNEGALLENAVLNYALDFLRERKFVQLSVPVLADEKYFIGTGYFPFMRSETFVAQ
                     DKDREFYLIGSAEVPLCGYYAGEILREEDLPVRLMALSPCFRTEVGSYGQDTKGLYRL
                     KQFYKVEQVVLCENDDQESKKMFDEILGNAEDFMQSLELPYQLSRLSTGEMGAGQVEK
                     FDIETWMPSRASYSETHSCSRFGDWQARRLNIRYKTRAGENKFVHTLNNTLIATPRIL
                     IPLLEINQQAGGTVDIPQILRKYMSGLSKIERK"
     gene            3043..>3402
                     /locus_tag="VGA49_03150"
     CDS             3043..>3402
                     /locus_tag="VGA49_03150"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9664798.1"
                     /translation="MSFLKIQNLNISYKISGQGQKILLLHGWGTDLSYFNDLVKLLVA
                     DEFQVVAVDLPGFGRSDSLDQGWSVDDYADFTNEFINNLNINDCYLLGHSFGGRIGIK
                     LAAGQPDWLKGLILVNSA"
BASE COUNT         1073 a          565 c          780 g          984 t
ORIGIN      
        1 ttgtttaaaa ctaaggctgg tgaactgacc gttcaggcca aaaagttcag gctgttaagt
       61 aaagctttat tgcctttgcc tgaaaaatgg catggcctga aagatattga agagcgttac
      121 cgcaaaaggt atcttgattt aataatgaac cccgaagtta aagaaagatt tgtcatccga
      181 tcaaaaatag tagaatcaat caggcggcta ctgcttaaca agggttatct ggaggttgaa
      241 acgccggttt tgcagacgat ctatgggggc gggtttgcca agccgtttat tactcatcat
      301 aacgctttgg atattccgct ttatttaaga atttctgacg agctttattt aaagcgattg
      361 ctggtcggtc attttgaaaa gatttttgaa ttttgccggg atttccgcaa tgaaggcctt
      421 gatccgtcgc ataatccaga atttacaatg ctggaggcga tgacggctta tcaagattat
      481 aggtttagca tggatttagt ggaagaaatt tatgaaacag ccgctaaaga ggttctgggg
      541 acgacaaaaa taaattacca ggaacatcag atagatttga aaaggccctg gaagcggctg
      601 actcttcatc aggcggtcaa agagtttacc ggacatgatt tggttaaagt cagtgattta
      661 tctgaagcca tagagatagc cgcgggtctt aaaattgata agcggaaaat taagccgctt
      721 cagtcggttg gcgaaatcac taatttgatt tttgaggaaa aggttgaatc aaatctaatt
      781 cagccggtaa ttatttatga ttatccggtg gaagtttcgc ccctggccaa aaaaagccgg
      841 actaatccta aagtcgttga gaggtttgag cattttattt tcggcaagga acacggtaat
      901 cattattccg agctgaatga tccggttgag ctcaaaaaaa ggtttattga ggagaaaaac
      961 aaaaaggaag ccggatttga agaagctcat cagacagacc ttgattttgt cgaagctttg
     1021 gagcatggca tgccgccggc caccggtatt ggcattggta ttgaccgttt ggcgatgctg
     1081 ttcactaatt cccggtcaat taaagaagta atttttttcc cgacgatgaa gcctaaaaca
     1141 aaatctattg aggaaaatgg aaaggccaaa tgaaaaaaat catggttttt gggacatttg
     1201 atattattca tcccggtcat cttaattttt ttgaacaggc aaaaaagcag ggtgattacc
     1261 tgatcgttgt cgttgcccgg gatacaaacg tccgcaaggt taaaagtttg tctccgcaaa
     1321 ataatgaaca ggttagactc aaacagctta aaaaaataat gattattgac cgggctgtcc
     1381 cgggtaatat tgatgatcct tataaagtaa tcatcgaaaa taagccggat attatttgtc
     1441 tgggttatga tcaaaaaaca tttgtcgacg gtctgctggc caaaatcaaa cagggtcaga
     1501 tcaaagacat taaaattgtc cgtttaaatt cttttaagcc ggaaaaatat aaaagttcta
     1561 aattaagaca taactatgct tgatattaaa tttatcaggg ataatcaaaa gttagttcag
     1621 gaaaatgctg gaaataaagg cattaagatt gacattaaaa agcttttaaa acttgatgat
     1681 cagcgccgaa aattaattac gaaaattgaa aaacttaaca gccagaaaaa tcaggccaac
     1741 caggaaatcg ctaaagctaa gggggctgat aaagcgaaaa aaatcaaaat gatgaaaaaa
     1801 gtggctgaca agatcagtca acttaaccct gaattgtcac aagtggaaaa agattattac
     1861 gaccaaatgt ctctggtgcc gcagattgtt gataagaaga ccccggttga tcctgaaggt
     1921 aaaaaaagta agattgaaag aagggttggc aacgttcccc attttaattt taagcagaag
     1981 gaccatctgg aattaggaag gcttctggat attattgacc tggaaagagg agtcagggtg
     2041 atgggaacca gaggttattt tttaaagaat gaaggggcct tgctggaaaa cgcggttttg
     2101 aactacgctc ttgattttct gcgggaaaga aaatttgttc agttaagcgt tccggttttg
     2161 gctgacgaaa aatattttat cggcaccggc tattttcctt ttatgcggag tgaaactttc
     2221 gtggctcaag acaaggaccg ggagttttat ctgatcggct cggccgaagt gcctctctgc
     2281 ggttattatg ccggcgagat tttaagagaa gaagatctgc cagtcaggct gatggcatta
     2341 tcgccctgtt tcaggactga agtcggcagt tatggccagg acaccaaagg tttgtaccgg
     2401 ctgaaacagt tttataaagt tgagcaggtc gttttatgcg aaaacgacga ccaggagtca
     2461 aaaaaaatgt ttgacgagat tttgggcaat gctgaagatt ttatgcagag cctggaactg
     2521 ccttatcagc ttagccggct gtcaactggt gagatgggcg ccgggcaggt tgaaaaattt
     2581 gatattgaaa cctggatgcc gtcccgggcc agttattctg aaacccactc ctgctcgcgg
     2641 tttggcgact ggcaggcccg gagacttaat attagatata aaaccagggc tggagaaaat
     2701 aaatttgtcc ataccctcaa taatacttta atcgccactc cgagaattct gatacccttg
     2761 ctggaaatta accagcaggc aggtggaact gttgatattc cccagatatt aagaaagtac
     2821 atgagtggtt tgtcgaagat tgaaagaaaa taaagttttc ttaaaaattc taaattcgaa
     2881 tatcgaaatc ctaaacaaat cataaattct aatgtcttaa attcaaaaca atatatatct
     2941 aatgtttgga gcattatgat ttgtaattta gaatttattt agtatttagg attttgatat
     3001 tagaattttt atgtttatag cttatattaa ttgttttaac aaatgagttt tttaaagata
     3061 caaaacctga atatcagtta taaaatttct ggtcagggcc agaagatttt attattacat
     3121 ggctggggga ctgatttgtc ttattttaat gatttggtta aattgctggt cgctgatgaa
     3181 tttcaggtag ttgctgttga tttgcctggt tttggtcgga gtgattccct ggatcagggc
     3241 tggtcagttg atgattacgc tgattttaca aatgagttta taaacaattt aaacatcaac
     3301 gactgttact tattaggcca ttcttttggc ggccggattg gcattaaact ggccgccggg
     3361 cagcctgatt ggttaaaagg gctaatcctg gttaacagcg ct
//
LOCUS       DASWHZ010000156         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1045341, whole genome shotgun sequence.
ACCESSION   DASWHZ010000156 DASWHZ010000000
VERSION     DASWHZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045341"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VGA49_03155"
     CDS             <1..303
                     /locus_tag="VGA49_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664799.1"
                     /translation="SILGLLVKTRSGFYLGRVADFDVDTVSHEVIRYYVRRPGLIKGA
                     FQKKLLIDKKQVISIIKEAMLVEDAVVTETEEIKKTQLAALGAEGPEINMSEELRN"
     gene            complement(382..1173)
                     /locus_tag="VGA49_03160"
     CDS             complement(382..1173)
                     /locus_tag="VGA49_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX9664800.1"
                     /translation="MYCYLYDTFLKDKKFLKTKLKIENRLTDLDLKGRSYQLTILNNA
                     REIAREAARQGAKTLVVIGDDKLTTKVIDVIAHYDDIALGIIPVGGDLKISKSFDIPA
                     GLAACDTLSGRIVKNISLGKINDFYFLDQISMPLQNTTVNFDEKYNIQTLNQNQSLEI
                     SNLNQRLAENNQRLEAIILETKDKKFFNFFGQKSQSSIFNIKRAAINGPSIPIKIDNQ
                     KVIKSPALIEVVPNKLRVIVGKKLNNQGTGQLKNGRTEQLGDGAT"
     gene            complement(1407..1805)
                     /locus_tag="VGA49_03165"
     CDS             complement(1407..1805)
                     /locus_tag="VGA49_03165"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX9664801.1"
                     /translation="MFNKDKNSKDNQAETIIGPSVKVEGDFTGEGNVIVEGVVNGNLT
                     TNQHLIVGPRAKIAANVVAASAVISGEVLGNIKVDEELELRGTSRVNGDLETPLLSIE
                     KGAVVNGKCCMVRKKEPAKLSDKPNEDENK"
     gene            2415..2486
                     /locus_tag="VGA49_03170"
     tRNA            2415..2486
                     /locus_tag="VGA49_03170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2448..2450,aa:Arg,seq:cct)
     gene            2705..3145
                     /locus_tag="VGA49_03175"
     CDS             2705..3145
                     /locus_tag="VGA49_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664802.1"
                     /translation="MFFSKERGYYYIDQLIHSRLIDPDFEEQLKEAINISFLPKKGQL
                     EPKIVYCDLGGCQHVFIYDINGEPHDVFSIQEAEEKLASMLKEGVITEDQYSRLTAEV
                     LETDLPVDLEENPGKLGKVIVLDISQFGLLLSKIIFGCGDPNLN"
     gene            3388..3654
                     /locus_tag="VGA49_03180"
     CDS             3388..3654
                     /locus_tag="VGA49_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9664803.1"
                     /translation="MKGYVYILQSIKNKRFYIGSTKNVENRLKQHQSGQVKATRNIRP
                     LQIVFQQEYPNIKQARQIEYRIKRYKNRKIITQIIADGEIKMGP"
     gene            3645..3718
                     /locus_tag="VGA49_03185"
     tRNA            3645..3718
                     /locus_tag="VGA49_03185"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3679..3681,aa:Ala,seq:ggc)
     gene            3790..3856
                     /locus_tag="VGA49_03190"
     tRNA            3790..3856
                     /locus_tag="VGA49_03190"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3816..3818,aa:Asp,seq:gtc)
     gene            3864..3937
                     /locus_tag="VGA49_03195"
     tRNA            3864..3937
                     /locus_tag="VGA49_03195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3898..3900,aa:Val,seq:gac)
BASE COUNT         1231 a          786 c          924 g         1269 t
ORIGIN      
        1 agcatattag gcttactagt taaaacccgc agcggctttt atttgggccg ggtggctgat
       61 tttgacgtgg acacggtcag tcatgaagtt atcagatatt atgttcggcg gccgggtttg
      121 atcaagggcg cttttcaaaa aaaattattg attgataaaa agcaggtaat ttccattatt
      181 aaagaagcaa tgctggttga agacgccgtg gtgacggaaa ctgaagaaat aaaaaagacc
      241 caactggccg cgctgggggc cgagggtccg gaaattaata tgagcgagga actgaggaac
      301 taaggaacag aagaacagag aaactaaaga ataaaagaac agaagaacag agaaacgaaa
      361 gaaccaatgg ttgttcagtt tctaagttgc tccgtcgcct agttgctcag ttcttccgtt
      421 cttcagttgc ccagttccct gattgtttag ttttttcccg actatcaccc gcagtttgtt
      481 cgggacaact tcgatcagag ctggcgattt aattactttt tgattatcaa ttttaatcgg
      541 aattgatggg ccgttaatag cggctctttt tatgttgaaa atgctggact gggatttttg
      601 cccaaaaaag ttaaaaaatt ttttatcttt agtttccaat ataatggctt ccaggcgctg
      661 attgttttca gccagccgct gatttaagtt ggaaatttcc aaagactgat tttgattaag
      721 cgtctgaata ttatattttt catcaaaatt aacagtggta ttctgaagcg gcattgaaat
      781 ttggtccaaa aaataaaaat cattgatttt gcccaggctg atgtttttaa cgatccggcc
      841 ggataaagta tcacaggcgg ccaggcccgc tgggatgtca aaagatttgg aaattttgag
      901 gtcgccgcca accggaataa tccccagagc gatgtcgtca taatgagcaa taacgtcaat
      961 tactttggtg gttagtttat catcgccaat gaccacaagc gttttagccc cctgccgggc
     1021 ggcttcccgg gctatttccc gggcgttatt taaaatggtc aattgataag accggccctt
     1081 taaatcaaga tcagtcagcc ggttttcaat tttgagtttg gttttaagaa attttttatc
     1141 cttaaggaag gtgtcgtaga gataacagta cataaaataa aatccaaaat taaaaatcct
     1201 aagctccaaa taaattcaaa attctccgcc aagggcggac ccgcctccgg cgggaaatgt
     1261 tcaaaatcca aaaaaagggt ggattttgaa tattagaatt taagatttat ttaggatttc
     1321 tcccgactga gtcgaggatc ctcgatggct tcgccatcgg gagaaattaa aatttagaag
     1381 ttaaaaagtt tttttaaaga aaaaggctac ttattttcat cctcgttcgg tttatccgaa
     1441 agcttagccg gttctttctt cctaaccatg cagcatttgc cattgacaac agctcctttt
     1501 tcaattgaca gcagaggtgt ttccagatcg ccgttgactc tggaagtgcc tcgtagttct
     1561 aattcctcgt caactttaat attgcccagg acttctccgg aaatgacggc gctggcggca
     1621 acaacatttg cggcgatttt ggcccgcggg ccaacaatca gatgctgatt ggtggttaaa
     1681 ttaccgttaa ccacgccttc aacaattaca ttgccctcgc cggtgaaatc tccttccact
     1741 ttgactgacg gaccaatgat tgtttccgct tgattgtctt tggagttttt atctttgttg
     1801 aacatttgag tgattttaat atttgattag ctttatctta gagcagatta tgccaatatg
     1861 caaattatta tgctaatatg caaatcttat gccaatcaac aaattgctaa tatttgcgtg
     1921 gataataccc gcttgacttg attttttttt gaaaaactgc tatattattg aaatctctgt
     1981 tcttttgagc ctgaaaagcg aagtggctct ttaagactag cccctcctcc gggttgattt
     2041 aaccccttta ttcctgcctc tcggggggtt aaattatcct ttagtcttat tgggccaggc
     2101 ctgccagcca ttacctcaag ctgaattgac tttatttgct agccgtcgcc agaccacgtt
     2161 ggtgtcatgg cctggtagct agacttaagt tgcccttggg atgttggact ggcaggctat
     2221 ttttatatca ggggagataa ggggtgatca agggacataa agggatatca ggggaataat
     2281 ccctgctaac ccgtgatgcc ccttgataat ccctgcttga tattttacga agcttgtttg
     2341 tatctatagt tagcgaatta atttttttat ataaaattta ttatctgcaa ttgtttcacg
     2401 tgaaacaatt ttatgccccc gtagttcaat ggacagaaca agggactcct aagccctaga
     2461 tgctggttca attcccgccg ggggcattga caaatataaa aaattttgat agaaataagg
     2521 gattttagcg aaaaaaagaa aaaatgccta aattagctgt tttgcctttt gttcgttgag
     2581 tctgtttgct tccggggagg tgccaatgtc aaattcaggg agatatcccc cctgtaaact
     2641 tatatttgct aaggccgaaa ctgggcctta cggggctatc ttttttggaa ataatcagca
     2701 gatagtgttc ttttcaaagg agcggggcta ttactatata gatcagctca tccatagccg
     2761 gttgattgat cccgactttg aagagcagct gaaagaggca ataaatatat cttttttgcc
     2821 aaaaaaggga cagcttgagc ccaaaattgt ctattgtgac cttggtgggt gccagcatgt
     2881 ctttatttac gatatcaatg gcgagcctca cgatgttttt tccattcagg aggctgaaga
     2941 aaagctggca agtatgctga aagaaggggt cattaccgaa gaccagtatt ctcgcctaac
     3001 ggctgaggtt ttagagactg acctgccggt tgatttggag gaaaaccccg gaaagctggg
     3061 taaagtgata gtcctggaca tcagtcaatt tggactgcta ctgtcaaaga ttatctttgg
     3121 ctgtggcgat ccaaacctca attaaggaga aaaagagctt tgcggagctc tttatttatt
     3181 ttctacctac atggggctct cttttttata taatcgatgc taatatacga atttatacta
     3241 atgaaactaa taaaaaaatg cccataaatg gagcttgatt ttttgaccat tttcagttat
     3301 aatgattgaa tcatttgtag tttgagggcc tatggcgcag ttggtagcgc gccccgatgg
     3361 acatcgggga ggtcagggat aataaatatg aaaggctatg tctatatttt acagagcatt
     3421 aagaataaaa gattttatat aggaagcaca aagaatgtgg aaaataggtt gaaacagcat
     3481 caatctggtc aagttaaggc aaccaggaat attcgcccac tacaaattgt atttcagcaa
     3541 gagtatccta atataaaaca agccagacaa atagaatata ggataaaaag atataaaaat
     3601 agaaagataa ttacacaaat aatcgcggat ggagaaatta aaatgggccc gtagctcagc
     3661 tggttagagc gtttgcatgg catgcaagag gtcaggagtt cgaatctcct cgggtccaaa
     3721 ttaaaattta cagaatgata cccttatacc ttggtcgttc ggccggtcgg gttcataatt
     3781 tgggcaatta gctcagttgg ttagagcgcc ccgatgtcca tcggggaggt cagcggttca
     3841 agtccgcttg ggtccaaaca tgggggttcg tagctcagtt ggttagagcg cttgctcgac
     3901 acgcaagagg tcagaggttc gaatcctctc ggacccaatt tgtaaaaata aaaagtaaaa
     3961 attcaaaagt aaaaactttg gaaatattta aaggcggctt gagtgcctct tttttatttg
     4021 aaattgtttc acgtgaaaca agggattttt attttgcgac ccacacctta atagacagtt
     4081 taaaaattta ttgttaagat taaaaaatat ttgtaatttg ctgattggcc taagatttgt
     4141 atattggcat aatttatatg gccgtaataa ctttaaacga tgacaacttt gaccaggaag
     4201 tcacctcctt
//
LOCUS       DASWHZ010000157         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2524813, whole genome shotgun sequence.
ACCESSION   DASWHZ010000157 DASWHZ010000000
VERSION     DASWHZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524813"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGA49_03200"
     CDS             complement(<1..511)
                     /locus_tag="VGA49_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX9664804.1"
                     /translation="MSRHQGHQPDYILIITFGSILLIGLIMLSSVSTAVAFDKFGDSY
                     YYFKHQLLFGVIPGLIILYILSRLNYHRWRKFAFPLLIITIGLLIAVFLPGIGFQLKG
                     AHRWISLGGILFQPSEIAKLTFLIYLASWLESKGERKLKDVDGGFLPFIFTLGTVMLL
                     IILQPDIGTT"
     gene            complement(620..2053)
                     /gene="murD"
                     /locus_tag="VGA49_03205"
     CDS             complement(620..2053)
                     /gene="murD"
                     /locus_tag="VGA49_03205"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9664805.1"
                     /translation="MNKEYFQGKKITIMGLGLHGGGLHIARWLARQGARLTVTDLKNK
                     KELETSLKKLKPYKNIKYVLGRHRAEDFQQADLIIQNPGVRRESKYLKLVRRYKIPVE
                     NEASLFFKLSPAPIIGVTGTRGKSTTTSLLYEIFKKHNPKTLMAGNIRQVVMFEVIDK
                     VKLKTPVILELSSWQLETLDDYQLSPHISLITNIYPDHLNRYKNLQDYVRAKKIIFKH
                     QQPDDLIVLNRDNFETKKLGKEVPGRRYWFSKKYFAEENGVFIKKGLICFRQNGREIR
                     IIKVKDVTIKGEHNLENVLGAVAVARISAIPVTVIKSVLKKYPGLDSRQELIRTYQGV
                     KFYNDTTATTPEAVIAALKTLAGNKNVILIAGGSDKKLAYGNLARAIEQSVKVLILLP
                     GTATKKIIKALHPTGDRPQGEKRKSIKTNGLFTTVDNIQNAVRLAFSAADKGDIVLLS
                     PGAASFGLFVNEFDRGQQFIETVQKLK"
BASE COUNT          619 a          490 c          430 g          643 t
ORIGIN      
        1 cggtcgttcc aatatccggc tgaagaatta ttaaaagcat cacagtcccc agagtaaaaa
       61 taaacggtaa aaaaccacca tcaacatctt tcagttttct ttctcccttg ctttccagcc
      121 agctggccag atagatcaaa aaagtcagct tggcaatttc actcggctga aataaaatac
      181 cgccaagact gatccagcga tgagcgcctt tcagctgaaa accaatcccc ggcaaaaaaa
      241 ccgctatcag caggccgatt gtaataatca gcaaaggaaa agcaaatttc cgccagcgat
      301 ggtaatttaa ccgggacaga atgtataaga ttattaaacc aggaataacg ccgaacaaca
      361 gctggtgttt gaagtaataa taactgtcac caaacttatc aaaagccacg gccgtgctga
      421 cagacgacaa cataattaag ccaatcaata agattgaacc aaaagtaatg attaaaatat
      481 agtctggctg atggccctgg tgtcgggaca tagggttgat taagattgat gaagtcccga
      541 tcccgccttt ggcgggatcg tggatcctcg atcccgccaa agacgggatc gggaggacaa
      601 aagggataaa ggggcaagat tacttcaact tctgtactgt ctcgataaat tgctggccgc
      661 ggtcaaattc attgacaaat aatccaaagc tggcggctcc gggcgacaat aagacaatat
      721 cgcctttgtc agccgcgcta aaagctaacc ggacggcgtt ttggatatta tcgacagttg
      781 tgaacagacc gtttgtttta atgcttttac gtttttcgcc ctgcgggcga tcacccgtag
      841 ggtgaagtgc ttttattatt ttctttgtgg ccgtcccagg caataaaatt aaaactttaa
      901 cagattgctc aatcgccctg gccaaatttc cataagccag ctttttatca ctgcccccgg
      961 ctattaaaat aacatttttg ttgccagcta aagttttcaa cgcggctata accgcttccg
     1021 gcgtggtcgc cgtggtgtca ttataaaatt taactccctg ataagttctg attaattcct
     1081 gccggctgtc taagccggga tattttttca gaaccgactt aatgacagtt accggtatcg
     1141 cggaaattct ggcgacagcc accgctccca aaacattttc cagattatgc tcccctttga
     1201 ttgtaacatc tttaacttta ataatccgga tctcccggcc gttttgtctg aaacagatca
     1261 ggcccttttt tatgaaaacc ccgttttctt cggcaaaata ttttttggaa aaccagtacc
     1321 gccggccggg aacttctttg cctaattttt ttgtttcaaa attatcccgg tttaaaacta
     1381 tcaggtcatc aggctgctga tgcttaaaaa taatcttttt ggctctgaca tagtcctgta
     1441 aatttttata acgattaaga tgatcaggat aaatattggt aattaaacta atatgagggc
     1501 taagctgata atcgtccaga gtttccagct gccagctgga cagttccaga ataaccggtg
     1561 ttttgagctt taccttatca ataacctcaa acattactac ctgccggata ttgccggcca
     1621 ttaaagtttt gggattatgt tttttaaata tttcgtaaag caaactggtg gtggttgatt
     1681 tgccccgggt gccggtaacg ccaataattg gcgccggaga cagtttaaaa aaaagactag
     1741 cctcgttttc aaccggaatt ttataccgtc ggactagttt taaatatttt gattcacgcc
     1801 ggacgccggg attttgaata atcaaatcag cctgttgaaa atcttccgcc cggtggcggc
     1861 ccaaaacata ttttatattt ttataaggtt ttaatttttt taaagaagtc tccagttctt
     1921 ttttattttt taaatcagtt actgtcagcc tggctccctg ccgggccagc cagcgggcta
     1981 tatgcaaacc gccgccatgc agccccaaac ccatgatagt tatctttttt ccttgaaaat
     2041 attctttatt catttttatt ttgaattctt aattccaatt actgataata agctccgttt
     2101 acggctgtcc ccttcccgcc gatgggaagg taaatttttc tggtcgcagt aagtgcaaac
     2161 atttgaaatt tccaaatttt cc
//
LOCUS       DASWHZ010000158         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1404587, whole genome shotgun sequence.
ACCESSION   DASWHZ010000158 DASWHZ010000000
VERSION     DASWHZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404587"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VGA49_03210"
     CDS             <1..589
                     /locus_tag="VGA49_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX9664806.1"
                     /translation="SQLISQAGLHQIHLAETEKYNQVTFYFNGKNGQPFKNEEWVLIP
                     SPQASDYADYPEMSAFEITSRAAREIRKKKYQFILINYCNPDIIGWSNNQAALKKSIE
                     IVDQCLGQIIPEALDHDGTVLITADHGQPGTINPVPLILIGKDWEGKSAPGGDTIGKD
                     LSLIKPAGGLADVAPTILKLLGIKKPAEMTGQPLI"
     gene            625..1494
                     /gene="mutM"
                     /locus_tag="VGA49_03215"
     CDS             625..1494
                     /gene="mutM"
                     /locus_tag="VGA49_03215"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX9664807.1"
                     /translation="MPELPEVETIRRGFKKYLTGKKIISVKVKVPKLVKLPVAQFKKL
                     LRGSKIKDVRRRAKLLIIDLSNKFSLLIHLKMTGQLVYRRPGGQLLAGGHPIKDGLEN
                     LPNKYSHIVFTFNDQSNLFFNDLRKFGWMKVIATDQVEKYLREVGYGPEPLDQAFTLK
                     KFKKMLAGREKSKIKALLLKQDFIAGLGNIYCDEACFYAKIHPLRTVKSLTDQEIKKL
                     YQGIKKVLKDSIELGGTSFDQYVNIDGARGGYVPRLKVYQRQGNPCRRCCTKIVKIKA
                     AGRGTHFCPKCQI"
     gene            1505..1666
                     /locus_tag="VGA49_03220"
     CDS             1505..1666
                     /locus_tag="VGA49_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664808.1"
                     /translation="MDCNSAREIYYFIQSGESKKIDQAKRAEATGHIQSCPECQALFP
                     EYAEEENGD"
     gene            1669..2199
                     /locus_tag="VGA49_03225"
     CDS             1669..2199
                     /locus_tag="VGA49_03225"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9664809.1"
                     /translation="MNIQLFRIINSWAGQNNFLDVFFVIGAKYVIFLIILIAVVFLFF
                     REYRRSAIRAFVVAAVAVSFNYLVLSSLFFRQRPYLILENVNKLVEHFSEKSFPSDHA
                     TVAFGVALTIFLFHRRIGLPLVILAVWVAFGRVFVGVHLPGDVVGGLIVALFFTLVIN
                     HKFGPRLRDNLKISIK"
     gene            2196..2576
                     /locus_tag="VGA49_03230"
     CDS             2196..2576
                     /locus_tag="VGA49_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX9664810.1"
                     /translation="MSKILKITGKVYRGQGKGRKLGFKTANLSPDSNQPFPPAGVYAS
                     LITIDDQPHKSVTAVGIFETFEQTKPGIEVYIFNFDQDIYNQPVDLELIKKIRPIKKF
                     LDASELKQQISRDCVEAMKILARF"
     gene            2586..3179
                     /locus_tag="VGA49_03235"
     CDS             2586..3179
                     /locus_tag="VGA49_03235"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9664811.1"
                     /translation="MFTGIIQSTGRVKKTEKNNQGGLILSVEVKNFLKNIKIGDSLAV
                     DGICLTVIKKSKNSFQTEVIPETLSRTIAPEYKKGSVVNLEKSLKIGDRLDGHFVPGH
                     VDGVGRLAKVTKVRDYLVWEIKPPVEILKYLAYKGSVAINGVSLTISRLNKNSFEVSL
                     ISHTLENTNLSELQPGFKVNIEADVIARYLEKLVVSS"
     gene            3199..3672
                     /gene="ribH"
                     /locus_tag="VGA49_03240"
     CDS             3199..3672
                     /gene="ribH"
                     /locus_tag="VGA49_03240"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9664812.1"
                     /translation="MRRNVFEKKISGRGFKFALIIARFNPPITDGLKNGALKALKANQ
                     VDEDDIDIYEVPGAFELPLIAQKTAVSKDHDAIICLGAVTKGETPHFHYVAKAVTDGV
                     VRVNLDTGKPVLFGVLTTDDISQAEARSADDDYNKGREAALAALEMVNLIKSENL"
     gene            3674..>4749
                     /locus_tag="VGA49_03245"
     CDS             3674..>4749
                     /locus_tag="VGA49_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9664813.1"
                     /translation="MNLVILCGGIGSRLWPMSRKNKPKQFFEIISDQPMIVDTYKRLA
                     GYYPADQLYFATTPEFVEEIKKIFPKVKDSNILIEPQRRDTGPAMGYAAARLFLKSPD
                     EPLAFIPSDHYIGNIKKFIKTLKVAEKLILETGRMIDISIEPNFPSTILGYTKLGDNH
                     QVIDGVDIYKFAGHTEKPDFETARKYLRTGNYLWHASYYMWTPRLFLDAYKKYAPEMY
                     QGLEKVMAGLKTGNQRLIEEEYSRLEKISIDYAITEKMDLADVLIIKGDFGWSDVGSW
                     DVLFDRLSAETDSDGNLLKGNCLSVDTKKTMVYGSPEKVTAIIGLSGMVVIDTKDALL
                     ICPTGKAQEVKKIIQKLKEEGLDQ"
BASE COUNT         1397 a          913 c         1087 g         1352 t
ORIGIN      
        1 aagccagctg atcagtcagg ccgggctgca tcaaattcat ctggctgaga ctgaaaagta
       61 caaccaggtg actttttatt ttaacggcaa aaacggtcag ccttttaaaa atgaagaatg
      121 ggttttaatt ccttcgcccc aggcttctga ttacgctgat tacccggaga tgtcagcttt
      181 tgaaatcact tcccgggcgg ccagggaaat cagaaagaaa aaatatcagt ttattctgat
      241 taattactgc aatccggata ttatcggctg gtcaaacaat caggcggctt taaagaaatc
      301 aattgaaata gttgatcagt gtctgggaca aattattccc gaagctctgg atcatgacgg
      361 aacagtttta atcaccgctg accatggtca gccggggaca atcaatccgg tgccgctgat
      421 tttgattggt aaagactggg agggaaaatc cgcgccgggc ggcgacacta ttggcaaaga
      481 tttaagctta atcaaaccgg ccggcggcct ggctgatgtg gcgccgacaa ttttaaaatt
      541 gttgggtatt aaaaagcccg ccgaaatgac cggtcaaccg ttgatttaac aacagccgct
      601 attttcgtta taattaaata aaatatgcct gaactgccag aagtggaaac tatccgccgg
      661 ggatttaaaa aatatctgac cggcaaaaaa attatttcag taaaagttaa agtgcccaaa
      721 cttgttaaac tgccggttgc tcaatttaag aaattattgc gtggttcaaa aatcaaagat
      781 gtccggcgac gggctaaact gctgattatt gatttatcca ataaatttag tctgttgatt
      841 cacctgaaaa tgaccggcca gctggtttat cgccggcctg gcggccagct tttggccggc
      901 ggccatccga ttaaagacgg cctggaaaac ctgccgaata aatacagcca cattgttttt
      961 acttttaatg accagagcaa tttatttttt aatgatttgc ggaaatttgg ctggatgaaa
     1021 gttatcgcga ctgatcaggt tgaaaaatat ctgcgggagg tcggttacgg acccgaaccg
     1081 cttgatcagg cctttaccct taaaaaattt aaaaagatgc tggctggccg ggagaaatcc
     1141 aaaattaaag cccttttgtt aaaacaagat tttatcgccg gcctggggaa tatttactgc
     1201 gatgaagcct gtttttacgc caaaattcat cctttgcgga cagttaaatc gctgactgac
     1261 caggaaatca aaaagcttta tcagggaatt aaaaaagttt taaaagattc aatcgagctg
     1321 ggcgggactt cttttgatca gtatgttaat attgacggcg cccggggcgg ttatgttccc
     1381 aggctgaaag tttatcagcg ccagggcaac ccctgtcgcc gctgctgcac aaaaattgtt
     1441 aaaattaaag ctgccggccg ggggacccat ttttgtccca agtgccaaat ttaattaaaa
     1501 atatatggat tgcaacagcg ccagagaaat ctattatttc atccagagcg gtgaatcaaa
     1561 aaaaattgac caggctaagc gggctgaagc gaccgggcac attcaaagct gcccggaatg
     1621 ccaggcttta tttcctgaat acgctgaaga agaaaacggt gattaattat gaatattcag
     1681 ttgttcagaa taattaatag ttgggctggt caaaataatt ttttagacgt tttttttgtg
     1741 attggcgcca aatatgttat ttttttgata attttgattg ccgttgtttt tttatttttc
     1801 cgggaatacc gccggtcagc catcagagcg tttgtcgtgg cggctgtcgc cgtttcgttt
     1861 aattatctgg ttttaagcag tttatttttt cgtcagcgtc cttatttaat tttagaaaat
     1921 gttaataagc tcgtggagca tttttctgaa aaatcttttc cgtcagatca tgccaccgtt
     1981 gcttttggcg ttgctctgac tatttttctt tttcatcggc gaattggttt gccgctggta
     2041 atcctggcgg tttgggtcgc ttttggccgg gtttttgtcg gtgttcatct gccaggcgat
     2101 gttgtcggag gtttaattgt cgctttattc tttacactgg tcattaacca taaatttggc
     2161 cctcgtttgc gggataattt gaaaatatcc ataaaatgag taaaatatta aaaataaccg
     2221 gcaaggttta cagaggccag ggcaaaggcc ggaaactggg ctttaagacc gctaatttat
     2281 caccagattc taatcagcca tttccgccgg cgggagttta cgcctcgttg ataaccattg
     2341 acgaccagcc ccataaatcg gttactgccg tggggatttt tgaaactttt gaacagacaa
     2401 aaccaggcat tgaagtttat atttttaatt ttgaccagga tatctataat cagccggttg
     2461 acctggagtt aattaaaaag atcaggccga ttaaaaaatt tttggacgct tcggagttaa
     2521 aacagcaaat tagccgggac tgcgttgaag ccatgaaaat attagccagg ttttaggtta
     2581 aaattatgtt cacaggaatc attcagtcaa ctggccgggt caaaaaaaca gaaaagaata
     2641 atcagggcgg cctgattttg tcagttgaag ttaaaaattt tttaaaaaat attaaaattg
     2701 gggatagttt ggcggtggat ggtatttgtc tgacagtcat taaaaaaagc aaaaatagtt
     2761 ttcaaactga agtcatcccg gaaactttat cgagaacaat tgccccggag tataaaaaag
     2821 gatcagtcgt caatctggaa aaaagtttaa aaatagggga caggctggac ggtcattttg
     2881 tcccgggcca tgttgacgga gtcggccggc tggcaaaagt cacaaaagtt agagattatc
     2941 ttgtttggga aatcaaaccg cccgttgaaa ttttaaaata tctggcttac aagggttcag
     3001 tcgccatcaa cggtgtcagc ctgacaatca gccgtttaaa taaaaatagt tttgaggttt
     3061 cactgatctc ccacactttg gaaaatacca atttgtcaga acttcaacca ggttttaagg
     3121 ttaatattga agccgatgtt attgccagat atttggaaaa attagtagtt agtagttagt
     3181 cattaaccaa taaccaatat gcgacgaaat gtttttgaaa aaaaaatcag cggccggggt
     3241 tttaaatttg ccctgattat cgcccggttc aatccaccga ttactgacgg tttaaaaaac
     3301 ggcgccctca aggctttaaa agctaaccag gttgacgagg atgatattga tatttatgaa
     3361 gtgcccggcg cttttgaact tcctttaatc gcccagaaaa cggctgtcag taaagatcat
     3421 gacgccatta tttgcctggg agcggttacc aaaggggaaa cgccccattt tcattatgtc
     3481 gccaaagccg tcactgacgg cgtcgtcaga gtcaatttag ataccggtaa gccggttttg
     3541 ttcggagttt taacgactga tgatatcagc caggctgaag ccaggtcggc tgatgatgat
     3601 tataacaagg gccgggaagc ggctctggcc gccttggaaa tggttaattt gattaaatca
     3661 gaaaatttat aatatgaatc tggtcatcct ttgcggaggg atcggctcca ggctgtggcc
     3721 aatgagccga aaaaataaac caaagcaatt ttttgaaatc atcagcgacc agccgatgat
     3781 tgttgatact tataaaagat tagccgggta ttatccggct gaccagcttt atttcgcgac
     3841 caccccggag tttgttgaag aaattaaaaa aatttttcca aaagttaaag attcaaatat
     3901 tctaattgaa ccccagcggc gggataccgg tccggccatg ggttatgccg ccgcccgttt
     3961 gtttttgaaa tcgcctgatg aaccgctggc ttttattccg tctgatcact atattggcaa
     4021 tattaaaaaa tttattaaaa ccctgaaagt ggctgaaaag ctgattttag aaaccggccg
     4081 gatgatagac atttcaattg agccgaattt ccccagcact attctgggct acactaaatt
     4141 aggcgataat caccaggtga tagacggcgt tgatatttat aaattcgctg gccatactga
     4201 aaagcctgat tttgaaaccg ccagaaaata tcttcggacc ggcaattatt tgtggcacgc
     4261 cagctattat atgtggacgc cccgtttgtt tctggatgct tataaaaaat acgcgccgga
     4321 gatgtatcag ggcctggaaa aagtgatggc aggcttaaaa accggtaatc agcgtttaat
     4381 tgaagaagaa tattccaggc tggaaaaaat ttcaattgat tacgccatta ccgaaaaaat
     4441 ggatttggcc gatgtcctga ttattaaagg tgattttggc tggagtgatg tcggcagttg
     4501 ggatgttttg tttgaccggc tgtccgctga aaccgattcg gacggcaatt tgctgaaggg
     4561 caattgtttg tcggttgata ccaaaaaaac catggtttac ggctcgcccg aaaaagtcac
     4621 ggcgatcatc ggcttaagcg gcatggtggt gattgatact aaagacgctt tattaatttg
     4681 tccgactggt aaagcccagg aggttaaaaa aattattcaa aaattaaaag aggaaggtct
     4741 ggatcaata
//
LOCUS       DASWHZ010000159         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3263347, whole genome shotgun sequence.
ACCESSION   DASWHZ010000159 DASWHZ010000000
VERSION     DASWHZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263347"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VGA49_03250"
     CDS             complement(<1..761)
                     /locus_tag="VGA49_03250"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9664814.1"
                     /translation="MVIADKDLKKLLLAYELVSQKDVDQALKTATEKEIDLEQALLDK
                     NLISDENLGQLVADFLGFPFVSLKKIHIADEVLKLIPESFAREKLVIVFERDKQGLTI
                     ALADPNDLEVSRFIGKKTGQKIKIHFATRADIQSALLHYRKNLKEEFENIINQKLAEV
                     KANNLSPEELAVYVPIIEIVNNLIDYAYNYNASDIHIEPRDKDVVIRFRIDGVLQDVL
                     TLSKDVLEMIVTRIKILSKLRTDEHLAAQDGKFIR"
     gene            complement(763..1137)
                     /locus_tag="VGA49_03255"
     CDS             complement(763..1137)
                     /locus_tag="VGA49_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9664815.1"
                     /translation="MPDKKTILIIEDDRFLSKIYSAKLEKEGYNLALAVDGEEGLRKL
                     AESKPDLILLDLILPGMSGFEVLDKIKSSSKTRKIPVIILSNLGQDEDIQKGQELGAD
                     DYLVKTDFSISEVINKIKSALA"
     gene            complement(1232..>1544)
                     /locus_tag="VGA49_03260"
     CDS             complement(1232..>1544)
                     /locus_tag="VGA49_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9664816.1"
                     /translation="NFEKKKDKIIFSVHDTGIGIPEKDCPKIFQKFFRASNVIKARSE
                     GTGLGLFIAKSIIESSGGEIWFESVEDDGTTFNFSLPINAGTSHKAQGTRLGKREQAI
                     A"
BASE COUNT          422 a          347 c          303 g          472 t
ORIGIN      
        1 ttacgaataa acttaccgtc ctgggcggcc agatgctcgt ccgtccgcaa ttttgataaa
       61 atcttgattc tggtgacaat catctccagg acgtctttgg ataaagtcaa aacatcctga
      121 aggacgccgt caatccggaa acgaatcacg acgtctttat cccggggctc aatatgaata
      181 tccgaggcgt tatagttata agcgtaatca atcaggttgt taactatttc aatgatcggc
      241 acgtaaacgg ccagctcctc cggcgataaa ttattagctt tgacttcagc cagcttctga
      301 ttaataatat tttcaaattc ttcttttaaa ttctttctgt aatgaagcag ggcgctctga
      361 atatcggccc gggtggcgaa atgaattttg attttttggc cggttttttt gccaataaac
      421 cgggaaactt ccagatcatt gggatcagcc agtgcgatcg tcaggccttg tttgtcccgc
      481 tcaaaaacga tcaccagttt ttcccgggcg aagctttccg gaattaattt caaaacttca
      541 tccgctatat gaatcttttt cagactgaca aagggaaagc ccaaaaaatc agcaaccaac
      601 tgacccagat tttcatcgga aatcaaattc ttatcaagca aagcctgctc taaatcaatc
      661 tctttttcag tggcggtttt aagagcctga tccacatctt tctggctgac caattcgtag
      721 gctaaaagca gtttttttaa atctttatca gcaatgacca tattaagcta aagctgattt
      781 aattttatta ataacttcgc tgatgctaaa atcggtttta accaaataat catcagctcc
      841 cagttcctgg cctttttgaa tgtcttcgtc ctgtcccaga ttagacagaa taatcaccgg
      901 gatcttcctg gttttagaac tgcttttaat tttatccaaa acttcaaaac cgctcatccc
      961 cggtaaaatc aaatcaagca gaatcaaatc cggtttggac tcggccagtt ttctaagacc
     1021 ctcctcgccg tcaaccgcca gcgccaggtt gtaaccttct ttttccagct tggcggagta
     1081 aatcttggac aggaaacggt cgtcttcaat gattaaaata gttttcttgt cgggcataga
     1141 tagaatgagg caataggcac gaggcaatag gcaatagggt tggtgtcagg ctgttgcctg
     1201 acaattaatt gagggcatcg ggcaaaagtt ttcaagctat tgcctgctcc cttttgccta
     1261 gccttgtgcc ttgtgccttg tgacttgtgc ctgcattaat tggtaatgaa aaattaaatg
     1321 tcgttccgtc gtcttcaact gattcaaacc aaatttcccc gccggaggat tcaatgattg
     1381 atttggcgat aaacaaaccc aggccggttc cttccgaccg ggctttaatg acattgctgg
     1441 cccggaaaaa tttctgaaaa attttaggac agtctttttc cggaatgcca ataccggtgt
     1501 cgtggactga aaaaattatt ttatcttttt tcttttcaaa atta
//
LOCUS       DASWHZ010000160         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1052566, whole genome shotgun sequence.
ACCESSION   DASWHZ010000160 DASWHZ010000000
VERSION     DASWHZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052566"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1535
                     /locus_tag="VGA49_03265"
     CDS             <1..>1535
                     /locus_tag="VGA49_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9664817.1"
                     /translation="LIFQIRQKVIKEGVLETAILENSAKLEKISGFNKRILDNVPVSI
                     LTMDKNGRIISANRYADILSGSPGKNKIGKNIFDFNLVKDNELEEDYHRLLREGASFD
                     KQDCIYQSESLTESKYLNILAVPLKNTAGEVEGAISMAVDNSKIVTSELKTKQKAEQL
                     FLLNQISLAINSVLDLKEVLWVILRNAAILTSAYQAELFLVEDEQLVLKEIYSLKYFY
                     NKNQQPKINFRLQFGQSIVGYVAQTKQPYLSSDVRIDPKFDKDNNDLEINSVLAIPIF
                     SQKKEDQLKGVLRVCSDRPNHFTEDDQEILTILANNASVAIANSELYNKVKSLKKFSD
                     DIIDSSSLAIVVTNQQGQIIRHNNAAAQFIKDLKLAQNDIFYVENYFNIIEIIKKIQQ
                     TKESFCLDNLIFPRDEKDYYYNIKIDPILLDDQLSSVIITIDDVTKKVKLEQEIRDLN
                     RNLEEKVALRTSELNLVNQKLEEAIKLKNRFIADASHELRTPLTIIKGHLDLIRHTKE
                     YSS"
BASE COUNT          505 a          282 c          309 g          439 t
ORIGIN      
        1 tttgattttt cagattcgtc agaaagttat caaagagggc gttctggaaa cagccattct
       61 ggagaattca gccaaactgg aaaaaattag cggttttaac aagaggattc ttgataatgt
      121 cccggtcagc attctgacaa tggacaaaaa cggccggatt atttcagcca accggtacgc
      181 tgatattttg tcgggcagtc ccggtaaaaa taaaatcggt aaaaatattt ttgattttaa
      241 tctggtcaaa gacaacgaac tggaagaaga ttatcaccgt cttttaaggg agggcgcttc
      301 ttttgacaag caggattgta tttatcagtc agaaagctta actgaatcca aatatctcaa
      361 tattctggcc gttcctttaa aaaatacagc cggcgaggtt gaaggagcaa tttccatggc
      421 ggttgataat tctaaaatcg tcaccagcga gttaaaaacc aagcaaaaag ccgaacagct
      481 ttttctgcta aaccagatta gtttggcgat caattcagtt ctggatctta aagaagtttt
      541 atgggttatt ttaagaaacg ccgctatttt gacttcagct tatcaggccg agctgttttt
      601 ggtggaagat gaacagctgg ttttaaaaga aatttacagt ctgaaatatt tttataataa
      661 aaatcagcag ccaaaaatta atttccggct acaatttgga cagagcatag taggttacgt
      721 ggctcaaacc aaacagccat atttatcaag cgatgtcagg attgacccca aatttgataa
      781 agacaacaat gatctggaaa ttaattctgt tttggctatc ccgattttca gtcagaaaaa
      841 agaggatcag ctgaagggag ttttgcgggt ctgcagcgac cggccgaatc actttacgga
      901 agacgatcag gaaattctga caattttggc taataacgct tcagtcgcca ttgccaacag
      961 tgaactttat aataaggtca agtctttgaa aaaattcagc gatgacatta ttgacagctc
     1021 ttctttggcg atcgtggtca ctaatcagca gggccagatt atcaggcata ataatgccgc
     1081 cgcccaattt attaaggatt taaagttggc acaaaacgat attttttatg tagaaaatta
     1141 ttttaatatc attgaaatta ttaaaaaaat tcaacagact aaggaatctt tttgtctgga
     1201 taatttaatt ttcccccggg acgaaaaaga ttattattac aatattaaaa ttgacccgat
     1261 cctgcttgac gatcagcttt ccagcgtgat tattactatt gatgatgtta ccaaaaaagt
     1321 taagctggaa caggagatcc gcgaccttaa ccggaatttg gaagaaaaag tcgctttgcg
     1381 aaccagcgaa cttaatctgg tcaatcaaaa actggaagaa gccattaagc tcaaaaaccg
     1441 ttttatcgct gacgcctcgc atgaattacg gacgcctctg acaatcatca agggacattt
     1501 ggatctgatt cgccatacta aagagtatag ttcca
//
LOCUS       DASWHZ010000161         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3604674, whole genome shotgun sequence.
ACCESSION   DASWHZ010000161 DASWHZ010000000
VERSION     DASWHZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604674"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VGA49_03270"
     CDS             complement(<1..344)
                     /locus_tag="VGA49_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664818.1"
                     /translation="MQIKTTSSDGVGFSLRGSPTDDKLIKIRPVKPTDEKMVKTFIET
                     VFLDKRVTDGLMTGGAIPIAPKNGLGAWKGDSLIGFVGAQTGTAKKSLPTLDYFGLSI
                     NRDLPGNNVKPE"
     gene            complement(422..1177)
                     /locus_tag="VGA49_03275"
     CDS             complement(422..1177)
                     /locus_tag="VGA49_03275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9664819.1"
                     /translation="MKDKAFWRFYSLVYDTLLRLIPYQKLLQRVIRAIGPPDQTLKIL
                     DAGCGTGNLEMFINQQAINAAIIGVDSTPAMLAIARKKNRASRKNCRFQPADLNQTLP
                     FEDSAFDVVVAINVLYALAQPLESLKEFWRILRPEGRLIIVTPKNKANLWPILGEHIR
                     LVQADLKSFWGRLVAWLKMGWLVANLLIVGLLNLIILKRGRDKKYHFYELPELEELVT
                     TAGFELEETDSDYGHQDWFLVSRKANGRVDKTA"
     gene            1508..2263
                     /locus_tag="VGA49_03280"
     CDS             1508..2263
                     /locus_tag="VGA49_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX9664820.1"
                     /translation="MFYFLLFILGLVFGSFINAWVWRLHVGKSVFKGYSICPRCQTRL
                     LIWDLFPVLSFLFLKGKCRHCFQPISRQYPLVELATALVFVLVYYLNTVSGLNWLVLI
                     RDLLIVVFLIAIFTIDYRYYLIPDKITLPAIIIVLVFNLVLGLNFLNMVIAAVVAGGF
                     FLVQFLISQGQWLGGGDIRLGVLMGVVLGWPLVLVGLFWGYVLGAIVGLILINFNKKT
                     FKSQVPFGTFLTVATFVTIYWGEWILKWYMGLF"
BASE COUNT          523 a          498 c          580 g          731 t
ORIGIN      
        1 tcctccggct taacgttgtt tcccggcagg tcccggttaa tcgagaggcc aaagtagtcc
       61 agtgttggca ggcttttttt ggccgtgcct gtctgggccc cgacgaagcc gattagggag
      121 tcgcctttcc aggcgccaag cccgtttttt ggcgcgatgg ggatcgcccc gcccgtcatc
      181 aatccatcag tcacccgctt atcaaggaag acggtttcga tgaatgtctt gaccattttt
      241 tcatcggtcg gctttaccgg ccgaattttg atcaatttgt catcggtggg gctccctctt
      301 agagaaaacc ccaccccatc agaagatgtc gttttgattt gcatcttcga gcccccttgg
      361 gttgggttgt tgttaaagaa cagctactaa ggaccgtcct tagtaggtaa atataaatta
      421 attaagcggt tttgtcaacc cggccatttg ctttgcgcga aactaaaaac cagtcctgat
      481 gaccgtaatc agagtcggtt tcttccagct caaacccggc ggtcgtaact aattcttcaa
      541 gctcggggag ctcgtagaaa tggtactttt tatccctgcc tcgcttgaga atgatcaggt
      601 tgagcagacc gacaatcagc agattggcca ccagccagcc cattttcagc caggcgacca
      661 gcctgcccca aaagcttttg aggtcagcct ggaccaatct gatatgttct ccaagtattg
      721 gccacagatt ggctttgttt tttggagtaa caataatcag ccggccttca ggccgtagaa
      781 tccgccagaa ttctttcagt gattccagcg gctgagccag ggcatacaaa acattaattg
      841 ccaccacgac gtcaaaggcg ctgtcttcaa aaggcagagt ttgattgagg tctgccggct
      901 ggaatcggca gtttttccgg ctagctctgt tttttttccg ggcaatggcc agcatcgccg
      961 gagttgagtc aactccaatg atggcggcat tgattgcctg ctggttgata aacatttcca
     1021 gattgccggt gccgcagccg gcgtcaagga tttttagcgt ctgatccggc ggaccaattg
     1081 ccctgatgac gcgctggagc agtttttgat aaggaattaa acgcaacagc gtgtcataga
     1141 ccagactgta gaatcgccaa aacgctttat ctttcatgag gaagtcctcc caaagatttg
     1201 attgtcaagg gacaaagcgc tggttaatcc agcactgcga aataatagca tattttataa
     1261 agggggtaaa gggggtcagg gaaaacaagg gaaatcaggg tagatgaagg tggatgaggt
     1321 cccgatcccg cctttggcgg gatcgtggat cctcgatccc gccataggcg ggatcgggag
     1381 ttgatgaggg tagattagaa tttttgcttc cctttgcttc cccttgattc cttttgattc
     1441 cctatgcttc cccttatatt cattgtataa taatactact agtgactaat gactagtggc
     1501 tgtctttatg ttttatttct tgctttttat tttaggcctt gtctttggca gttttattaa
     1561 tgcttgggtc tggcggctcc atgtcggcaa gtcagttttt aaaggctatt caatttgtcc
     1621 ccgatgccag acccgcctgc tgatctggga cttgtttccg gttttaagtt tcttattttt
     1681 aaaaggcaaa tgccgccact gttttcagcc gatttccagg cagtatcctt tagttgaact
     1741 ggcaacagca ttagtttttg ttttagttta ttatctcaat acagtttcag gtttgaattg
     1801 gctggttttg atcagagatt tattgattgt cgttttttta atcgcgattt ttacgataga
     1861 ttatcgttat tatttaatcc cggacaaaat taccctgccg gcgattatta ttgttttagt
     1921 ttttaacctg gttcttggtt tgaatttttt aaatatggta attgccgcgg tggtggctgg
     1981 cggttttttc ctggtccagt ttttaatttc ccaaggccag tggcttggcg gcggcgatat
     2041 ccggctggga gttttaatgg gagtcgtcct gggctggccg ctggttttgg ttggtttatt
     2101 ttggggttat gttctcggag cgattgtcgg tttgatttta ataaatttta ataaaaaaac
     2161 attcaaaagc caggtgcctt tcgggacatt tctgacagta gcgacgttcg tgacaattta
     2221 ttggggtgag tggatattga aatggtatat gggactgttt taaagggtga tcagggatta
     2281 tcaggggata gcaagggaaa tcaaggatag tttttcccta atgttccttt at
//
LOCUS       DASWHZ010000162         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3605239, whole genome shotgun sequence.
ACCESSION   DASWHZ010000162 DASWHZ010000000
VERSION     DASWHZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3605239"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VGA49_03285"
     CDS             <1..1189
                     /locus_tag="VGA49_03285"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9664821.1"
                     /translation="KPVEEAEYDINGDGQKEKIKDLTNEKDIKSFKRTYGFDVRTKAC
                     DTLRNILPIATKTNVGLFGNGRFYQNILIHLYSSPYGETQDMARQAHQELNKLIPQYV
                     KRAKKNDYLVANHQNTRTLATELFKDIQPETDEAVVLLDKGEDTLANLINQKIQSGHK
                     LTENDLRQMNQAEQDNFTVASMLYPYLGHPFRQIRKKVRELPDETKQKIIKTYVGQRG
                     HRRNKPSRAFEMEYSYIFDLVTDWGTYKDLMRHRMNSQLRQQFTPKLGFTMPQDLIEA
                     GYDHLAQKCVDRAIELYDLLVKDFTAEASYATLHGCKTRWLLGMNCREAFHLLELRST
                     PQGHPLYRQAAQKMHQLISDRCQWRGEIMEFVDHNDYFWSRADSEARQRVKEKELDEK
                     YKS"
     gene            1271..>2392
                     /locus_tag="VGA49_03290"
     CDS             1271..>2392
                     /locus_tag="VGA49_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9664822.1"
                     /translation="MSLIRTQLEIISLLDSPLAPYAVQNSQSQGREFSEPADRRLPFQ
                     RDKDRIIHSKAFRRLKLKTQVFVSGRGDHFRDRLTHSLEVSQISRGLARSLGLNEDLT
                     EAVALAHDLGHTPFGHAGEEALNEMMAQFGRQFKHNQQSKRIVEKLEKKYPDFEGLNL
                     TWEVRNGLMKHDTPWEKGTSLPHTFHLEAQIANVADEIAYIGHDIDDGIRAGIIKIAD
                     FKKTNFGQEIRSKVKQRYPNPDLSPKILIQQIQRLTVNSLVDDILKTAGLNLEKFKIQ
                     SLSDVLNLSQPLIAFSPGQTEKNKEIHNFLHKNFYQHPQVIEAQTKGQEIIKKLFNYY
                     LNQPEQLPAGYQKHINNPEPVEIIIKDYVAGMTDNFARQL"
BASE COUNT          773 a          551 c          494 g          574 t
ORIGIN      
        1 taaaccggtt gaagaggctg aatatgacat taatggcgac ggtcagaaag aaaaaattaa
       61 agatctgaca aatgaaaaag acattaaatc ttttaaaaga acttacggct ttgatgtcag
      121 aaccaaagcc tgcgacactt taagaaacat cctgccgatt gccaccaaaa ccaatgtcgg
      181 cctgtttggc aacggccggt tttatcagaa tattttaatt cacctctaca gctcgcctta
      241 cggcgaaacc caggatatgg cccgacaagc ccatcaggaa cttaacaaac taatacccca
      301 gtatgtcaaa cgggccaaaa aaaatgatta tctggttgcc aatcatcaaa atacgcgaac
      361 tttggcgact gaacttttca aagatattca gccggaaacc gatgaagctg tcgttctgct
      421 ggacaaagga gaagacactc tggctaatct gattaatcag aaaattcaat cgggccacaa
      481 actaactgaa aatgatctaa gacaaatgaa ccaggccgaa caggataatt ttacggtagc
      541 ctccatgctc tacccctatc tcggccaccc tttcaggcaa attaggaaaa aagtcagaga
      601 actgcctgac gagaccaaac aaaaaattat taaaacttat gttggtcaaa ggggtcatcg
      661 ccgtaataaa ccatcccggg cgtttgaaat ggaatacagt tatatttttg atctggtgac
      721 tgactgggga acttataaag atttaatgcg ccaccggatg aacagccagc tccgccagca
      781 attcactcca aaactcggct ttaccatgcc ccaagatttg attgaggctg gttacgatca
      841 tttggctcaa aaatgcgttg accgggcgat tgaactttat gatcttttag ttaaggattt
      901 cacggctgaa gcctcctatg ccacgctcca cggctgtaaa acccgctggc tgctcggcat
      961 gaactgccgg gaagcttttc atttgttgga attaagatca actccccagg gccacccgct
     1021 ctaccggcaa gccgcccaga aaatgcacca gttaatcagc gaccgctgcc agtggcgggg
     1081 cgagattatg gagttcgtcg atcacaatga ttatttctgg tcgcgggctg attcagaagc
     1141 caggcaaagg gttaaggaaa aggaattgga tgagaaatat aaatcttaaa aatttctaaa
     1201 tcataatatc gaaattctaa ataaatccga aatttagata tttactaaca aagatattag
     1261 catttacaaa atgtccctca taagaactca attagaaata atttccctcc tggactcacc
     1321 cctggctcct tacgccgttc aaaatagtca gagccaggga agagaattct cggaaccagc
     1381 tgaccggcgg ctccctttcc agcgggacaa agaccggatt attcattcca aagctttccg
     1441 ccggttgaaa ctcaaaaccc aggtttttgt cagcggccgc ggcgatcatt tccgcgatcg
     1501 tttaactcac agcctggaag tcagccaaat cagccggggg ctggcccgct ctctgggact
     1561 gaatgaagac ctgactgaag ccgtcgctct ggctcatgat ctgggacata ctccgttcgg
     1621 ccacgcgggc gaggaagctt tgaatgaaat gatggcccag tttggccggc aatttaaaca
     1681 caatcagcaa tcaaaacgaa tcgtcgaaaa gctggaaaaa aaatatcccg actttgaagg
     1741 acttaatttg acctgggagg tcagaaacgg cctgatgaaa catgacaccc cctgggaaaa
     1801 aggaacgtca ctcccccaca cttttcatct ggaagcccag atcgccaatg tggctgatga
     1861 aatcgcttat attgggcatg atattgatga tggcatcaga gctggtataa ttaagatagc
     1921 tgactttaaa aaaaccaatt ttggccagga aatcagatca aaggttaaac agcgctatcc
     1981 taatccggac ctttctccaa aaatattgat tcaacaaatc cagcgactga cagttaatag
     2041 tttagtggat gacattttaa aaacagccgg gttaaatctt gaaaaattta aaatccagtc
     2101 tttaagtgat gttttaaatc ttagtcagcc gttaattgct tttagccccg gccaaactga
     2161 aaaaaacaag gaaatccata actttttaca caaaaatttt taccagcatc cccaggtaat
     2221 tgaagcccag accaaaggcc aggaaattat caaaaaactc tttaactatt atcttaatca
     2281 gccggaacaa ctgccggcag gctatcaaaa acacataaat aacccggagc cggttgaaat
     2341 aatcattaaa gactatgtcg ccggcatgac tgataatttt gccagacagc tt
//
LOCUS       DASWHZ010000163         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1055243, whole genome shotgun sequence.
ACCESSION   DASWHZ010000163 DASWHZ010000000
VERSION     DASWHZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055243"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..2149)
                     /locus_tag="VGA49_03295"
     CDS             complement(359..2149)
                     /locus_tag="VGA49_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX9664823.1"
                     /translation="MTELKIILKNNFNFNDFLPGQEEIIRSVLDTNQTVAIMPTGGGK
                     SLCYQLPALVFDNLTLVISPLIALMKDQVDSLKKLKIPATFINSSLTPTEYQSRINNL
                     KEGKYKLLYVAPERFYDQNFVSLLNNLKISLMAIDEAHCISEWGHDFRPSYLRIKDVI
                     PTLLFEGGQPRVVALTATATPEVRKDIIKQLNLDAPRVFIAGFNRDNLKLTSIQTSSY
                     QKLGIIHDLIKKATGPAIIYAGTRNKVEEITSFLRDFDIKANGYHAGLDNQNRQKIQE
                     YFMNGDNDVIVATNAFGMGVDKPDIRLVIHHDMPGNLEAYYQEIGRAGRDGRPSECVL
                     LYSPADRNLNEFFLAGDNPSPQTIAEIYKFLLKQDFDETEPTIYVTQKEIAQSLSENV
                     SEMAVSTAIKIMEREGYLQRLREKNNDAGVKLNRPLQEIKQFIGNRAKVQLTIIESLV
                     KDFEANQENEIKFAVEELAAKNGLEKSAVQRGLNALAKNFLIDYNPPFKGRGVKLLKS
                     EPLEIDYQKLEAKRQRELDKLNIMENYIHSDQCKHQYILEYFGEDGTIQSCGEMCDNC
                     LMEKSSKLIGSQNQTDGILDKLLHIRNQRE"
BASE COUNT          586 a          481 c          522 g          714 t
ORIGIN      
        1 cctgggcttt ggggtgaatc ggcacgccgg gattgataaa actgctgaac tgatcaacga
       61 tttgatcgcc ttttattttc aaaccggcga tttcaataat cccgtcgccc tgataagggt
      121 agaggccggt ggtttcgaga tccaggatta cgaattgagg tgaaatggtg gaagtcataa
      181 ggaaataagt agaagcaagg ataaaaattt taaaatccag ctattaaatc caaatatcaa
      241 agctcaaata tcaattgaaa tccaaagttc aaatgtcaaa tagtaaaatt tttggatttt
      301 agattttgga tttgaattga catttggatt ttgtaatttg aaatttaata gcttattctc
      361 attccctctg attccttata tgtaataact tgtccaggat gccatcagtc tgattttggc
      421 ttccaataag cttactgctt ttttccatga gacaattatc gcacatttct ccacacgact
      481 gaatcgtccc gtcctcccca aaatattcca aaatatattg atgcttgcac tggtcgctat
      541 ggatataatt ttccattata tttaatttat ccagctccct ttgccttttg gcttccagtt
      601 tttgataatc aatttccagc ggctcgcttt tgagcagttt cactccccgg cctttgaaag
      661 gggggttgta gtcaatcagg aaatttttag ccagcgcgtt aagtccccgc tgaaccgctg
      721 atttttccag gccgtttttg gcggccagct cttccacggc aaatttaatt tcattttctt
      781 gattggcttc aaaatctttg accagcgatt caataatcgt cagctgaact ttggcccggt
      841 tgccgataaa ctgtttgatt tcttgtaatg gccgattgag tttgacgccg gcgtcattgt
      901 ttttttcccg gaggcgctgg agataacctt cccgctccat aattttaatg gctgttgaaa
      961 cggccatttc actgacattt tcgcttaaac tctgggcgat ctctttttgg gtaacataaa
     1021 tggttggttc ggtttcgtca aaatcctgtt tgagtaaaaa tttataaatt tcagcgatcg
     1081 tctgggggga gggattgtcg ccggccagaa agaactcgtt taaattccgg tcagccggac
     1141 tatagagcaa aacgcactca ctgggccggc cgtcccggcc ggcccggccg atttcctggt
     1201 aataagcttc cagattgccg ggcatgtcgt ggtgaatcac cagccggatg tctggtttat
     1261 caacgcccat cccaaaagcg ttagtggcca cgatgacatc attgtcgcca ttcataaaat
     1321 attcctgaat tttttgacga ttctgattgt ccagaccggc gtgatagccg ttagctttga
     1381 tatcaaaatc ccgcagaaaa gaggtgattt cttccacttt gttcctggta ccggcgtaaa
     1441 taatcgccgg gccagtagct tttttgatca gatcgtgaat tattcccagt ttttgataag
     1501 acgaagtctg gattgaagtt agttttaaat tatcacgatt aaagccggcg ataaatactc
     1561 gcggggcgtc caggtttaat tgtttaataa tgtcttttct gacttcgggc gtggccgtcg
     1621 ctgtcagggc cactacccgt ggctgaccac cttcaaagag taaggtggga ataacatctt
     1681 tgattcgcaa atagcttggc ctaaaatcat gaccccactc ggaaatgcag tgggcttcgt
     1741 caatggccat cagggaaatt tttagattat tgagcaggct aacgaaattt tgatcgtaaa
     1801 atctttccgg cgccacatag agcagtttat attttccttc tttaaggtta ttgattcggg
     1861 actgatattc tgttggagtc agcgaactgt tgataaaagt cgccgggatt ttcagttttt
     1921 tcaaagagtc aacctgatct ttcatcagag cgatcagcgg cgagatgacc agagtcagat
     1981 tatcaaacac cagagccggc agttggtaac acaaagattt gccgccgccg gtcggcataa
     2041 tggccacggt ctggttagtg tccagaacgc ttctgatgat ctcttcctgt ccggggagga
     2101 agtcgttgaa gttgaagttg tttttgagga ttatttttag ttcagtcatg tttttttaca
     2161 aaaaagcccc gtggggcggt tgggggtaca aatataatag catattttaa atgtaaaaaa
     2221 aaccccccgc gcagttttga gtgaaactat gcggggggag gcgaaatcag ggttctgggt
     2281 ttagtcccag tattctttcc agg
//
LOCUS       DASWHZ010000164         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1807121, whole genome shotgun sequence.
ACCESSION   DASWHZ010000164 DASWHZ010000000
VERSION     DASWHZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807121"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VGA49_03300"
     CDS             <1..642
                     /locus_tag="VGA49_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9664824.1"
                     /translation="IPVLLMVVYTFQKNETKFDRPAKIGQVARLIFKRKSLKSIFYIS
                     FLLQFFYSWMVIYTPLYLRSLDFSWELIGQIFTVMLLPFVFFQFPVGYLADRYFGEKE
                     MLTLGLVIMSLATVGLFFISTPLAIGAVLFLTRVGASTVEILRDSYFYKQINAAEIDL
                     IDYFRNTGPLAYIISPLIATGLLLFLPMSALFIILGLFCLTGLIFSLTMPDTK"
     gene            658..1944
                     /gene="miaB"
                     /locus_tag="VGA49_03305"
     CDS             658..1944
                     /gene="miaB"
                     /locus_tag="VGA49_03305"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX9664825.1"
                     /translation="MKSYHIHTLGCQMNKSDSERISALLNKGGLKECLQEEEADLIIV
                     NSCSVRQKAIDRIYGKLRKWNGWRQDRGLKTILTGCVLEKDKNQMSDSFDFVVNIKDI
                     VKIPELLNLGRAEEESVCQTTDYLEIKPDYKSKFQAFVPIMTGCNNYCSFCVVPYTRG
                     LETSRPVDSIISEIKNLVSQGCKEITLLGQNVNSYKGQVDFPGLLRKINELDGDFWVK
                     FLTSHPKDMSDELIKAVADCDKLCDYIHLPVQSGDNQILKKMNRSYTVEHYQGLIEKI
                     KKQISGVVISTDIIVGFPTETDEQFQKTVDLFKAVSYEMAYISQYSPRSNTLAVKLFK
                     DDIPHEVKKTREKTLDEVLAKIALEKNKKLINTIQPVLVEKNEQSADDLYVNFGHTKN
                     HKLVKFESGRQVLGQVVDLKIIEAGAWGLFGILAKA"
     gene            2143..>2780
                     /gene="miaA"
                     /locus_tag="VGA49_03310"
     CDS             2143..>2780
                     /gene="miaA"
                     /locus_tag="VGA49_03310"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9664826.1"
                     /translation="MNKLIVILGPTGSGKSKLAVKLAKKFKGEIVSADSRQIYQEMDI
                     GTGKITKAEMAGVKHYLLDIVRPDQDFSLADYKKRALLAIKEIHQKNKLLFLVGGTGL
                     YIQAVVDNLSIPKVAPDKRLRKKMENELRTKGSRSLYIKLLKLDPLAKDIVDPKNPRR
                     VIRALEVCLKTKEPFSGFYQRGQPLFDFLLIGPKIDRQKLYQKIDRRVDQMV"
BASE COUNT          793 a          509 c          648 g          830 t
ORIGIN      
        1 attccagttt tgctgatggt ggtttacact tttcagaaaa atgaaaccaa atttgaccgg
       61 ccggctaaaa tcggccaggt cgcccggctg atttttaaga ggaaatcttt aaagagtatt
      121 ttttatattt cttttctgct ccagtttttt tattcctgga tggtgatcta cacgccgctt
      181 tatttaagaa gccttgattt ctcctgggag ctgattggcc agattttcac ggtaatgctt
      241 ttgccgtttg ttttttttca gtttccggtc ggctatctgg ccgatcgata ttttggcgaa
      301 aaagaaatgc tgactttggg gctggtgatt atgagtctgg cgacggtcgg tttatttttt
      361 atttccacgc ctttggccat tggggccgtt ttgtttttga ccagagtcgg cgccagcacc
      421 gttgagattt taagagattc gtatttttat aagcagatta acgcggccga gatagatttg
      481 attgattatt ttcggaatac cgggccgctg gcttatatca tttccccgct gatcgccacc
      541 ggcctgttgc tgtttttgcc gatgagcgct ttatttatta tattaggact attttgcctg
      601 accggtttaa tatttagttt gacaatgccg gatacaaaat aaaattaaaa atttaaaatg
      661 aaaagttatc atattcacac tttgggctgt cagatgaata aatctgattc agaaagaatt
      721 tccgccctgc tgaataaggg tggtttgaaa gaatgcctcc aggaagagga ggctgatttg
      781 attattgtta attcctgttc tgtccgtcaa aaagcgattg accggattta cggcaaactg
      841 cgaaaatgga acggctggcg gcaggatcgg ggactgaaaa caatcctgac tggctgcgtt
      901 ctggaaaaag acaaaaatca aatgtcggac agctttgatt ttgttgtcaa cattaaagac
      961 attgtcaaaa ttcccgaatt gttaaatttg ggccgggctg aagaagaatc tgtctgccag
     1021 acaactgatt atctggaaat taagccggac tataaatcaa aattccaggc gttcgtgccg
     1081 attatgaccg gctgtaataa ctactgcagt ttttgcgtgg tgccctacac ccggggtttg
     1141 gaaacttccc ggccggttga ttctattatc agtgagatta aaaatttagt cagccagggc
     1201 tgtaaagaaa ttacattgct gggccagaat gttaattctt ataaaggcca ggttgatttt
     1261 ccgggccttt tgagaaagat taatgaactg gacggcgatt tttgggttaa gtttctgact
     1321 tcccatccca aagatatgtc ggacgagctg attaaagcgg tggctgactg cgacaagctc
     1381 tgcgattaca ttcatctgcc ggtccagtca ggcgataatc agattttaaa aaaaatgaac
     1441 cggtcatata ccgttgagca ttatcaggga ttaattgaaa aaattaaaaa acagatttcg
     1501 ggcgtagtta tttcaaccga tattattgtc ggttttccaa ctgaaactga cgagcagttt
     1561 cagaaaacag ttgatctatt caaagccgtt agttatgaga tggcttatat ttcccaatat
     1621 tcgccccgga gcaatacttt ggcggttaag ctttttaaag atgatattcc tcacgaagtt
     1681 aaaaagacaa gagagaagac tttagacgag gttttggcaa aaattgcttt ggagaaaaac
     1741 aaaaagctga tcaatacgat ccagccggtc ttggttgaaa aaaatgagca gtcagccgat
     1801 gatttatacg ttaattttgg ccatactaaa aatcataaac tggttaaatt tgaaagcggc
     1861 cggcaggtgt tgggacaggt ggttgatttg aaaattattg aagctggcgc ctgggggttg
     1921 tttggtattt tggccaaggc gtaaatcaat tattcacttg ctgcttgtcc gtcgcttgac
     1981 agcgacggtt ttggttttag ggcttgggtg tggcagctag tatggttttt gggtttgggt
     2041 tgaggtaaaa aaaaccaaca ctaaaaccac agaccatacc agctgccaaa accagacccc
     2101 aaacccagat cattttaatt actaaaacta aaaaatattg ctatgaacaa gttaatcgtt
     2161 attctgggtc cgaccggatc aggcaaatct aagctggcgg ttaagctggc taaaaaattc
     2221 aaaggagaaa ttgtctcggc tgattcccgc cagatttatc aggagatgga cattggcacc
     2281 ggtaaaatta ccaaagcgga aatggccggc gtcaaacatt atcttttgga tattgtcagg
     2341 ccagatcagg atttcagcct ggctgattat aaaaaaagag ccttgctggc cattaaagag
     2401 attcatcaaa aaaataaact gctttttttg gttggcggga caggtttata tattcaggcg
     2461 gtcgttgata atttatcaat tcccaaagtg gcgcctgaca agcgcctgcg taaaaaaatg
     2521 gaaaatgaat tgcggaccaa aggttcgcgg tcactctata ttaaattgtt aaaactggat
     2581 ccgctggcca aagatatagt tgatccaaaa aatccccggc gggtgatcag ggctttggaa
     2641 gtttgtctga aaaccaaaga acctttcagc ggtttttatc agagaggcca gccgttgttt
     2701 gattttttgc ttatcggccc gaaaattgat cggcagaaat tatatcaaaa aattgaccgg
     2761 cgggttgacc agatggttaa
//
LOCUS       DASWHZ010000165         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3267605, whole genome shotgun sequence.
ACCESSION   DASWHZ010000165 DASWHZ010000000
VERSION     DASWHZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267605"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..670)
                     /locus_tag="VGA49_03315"
     CDS             complement(62..670)
                     /locus_tag="VGA49_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX9664827.1"
                     /translation="MFEKINIMGRKIREVILKPFLTVLVKAGISANLISTAKIVLMAP
                     FFYLVDKNLFWAGAMLIIALMLDGLDGPLARLAREQSDRGKFFDLLSDHIIYSVEILA
                     VIYLGLVDGLTGAYQIFIIAVIYLLAIIKKNEQLKSDWLISPSPRIIYYKVFFNVIFA
                     LYLFWQINFLQPALFYLNIIMTLAGVYYYFIIVLRKWKKSDR"
     gene            707..>2010
                     /locus_tag="VGA49_03320"
     CDS             707..>2010
                     /locus_tag="VGA49_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664828.1"
                     /translation="MKTFYKYIIIFFILIPIIIVNLFNWQSVNIGLIFGLAYLYLLAD
                     ITGNKFFGRLDACFKYIFGLLVSLSLIIILGTGFYYVWKFDLAMMSLLIIGLPLILIL
                     AEKYLPSPLNLPAEITGPAQTTDWRIFALGIFYLALITVSFKMLFSGQTTASLRSPWE
                     VIPSDFFIVYFLATLILILIIFFNKKIKLSLLLTSLHFFLSLSVGLIIYKIGFGFDPF
                     IHRAAEKFILNAGVITPKTFYYIGQYSLVTFLARLLQIPVELIDKLILPLLSAIYLPA
                     IVYFSLSRLDFFKNKQVIVFLSLLFLAVPYAGLTVTTPQSLANLFLVLVIFLSLNLVL
                     KSDYQSPVWLLFLLASASLLIHPLAGIPAFIFLTLLIYLTKSEEIQLKSFFKKAIFWE
                     IVAVSSVILPLIFLGLSFLSGQLTTAFKTDLINLPRIDLTRN"
BASE COUNT          572 a          434 c          346 g          658 t
ORIGIN      
        1 taaatcagga caaatataag agccagcgct gataagatca ggaaataacg gtattcttta
       61 attatctgtc tgattttttc cattttctca aaacaataat gaagtaataa taaacgccgg
      121 ccagcgtcat aataatattt aaataaaata aagctggctg taaaaagtta atttgccaaa
      181 aaagatacaa ggcaaaaatg acattaaaga aaaccttata ataaataatc cggggactgg
      241 ggctaatcag ccagtcagat ttcagctgct cattcttttt aattatcgcc agtaaataaa
      301 tcacggcaat aataaaaatc tggtaagctc cggtcagacc gtcaaccaga cccagataaa
      361 tgacagccag aatctcaact gaataaatta tgtgatcact caaaagatca aaaaattttc
      421 ctctgtctga ttgctcccgg gccagcctgg ccagcggacc atccagtcca tcaagcatca
      481 gcgcgataat cagcatcgcc cccgcccaga ataaattttt atcaactaaa taaaaaaagg
      541 gcgccattaa aacaattttg gctgtgctga ttaaattggc tgaaatgcca gccttgacta
      601 agacagtcaa aaatggtttt aaaatcacct ctctgatctt tcttcccatg atattgattt
      661 tttcaaacat aaataatcaa tttatgttaa aataaacctg aaaaatatga aaacttttta
      721 caagtatatc ataatatttt tcattttaat accgataatt attgtcaatt tgttcaactg
      781 gcagtctgtt aacattggct taattttcgg actggcctat ctttatctgc tggctgacat
      841 tacaggcaat aaatttttcg gccggctcga cgcctgcttt aaatatattt ttggattact
      901 ggtcagcctg tccctgataa taattctggg aacaggtttt tattatgtct ggaaatttga
      961 tttggcgatg atgtctttat taatcatcgg cctgccgctg attcttatcc tggctgaaaa
     1021 atatctgcct tcaccgctta acttgccggc ggaaataacc gggccggctc aaacaaccga
     1081 ctggagaata tttgctttgg gaatttttta tttagcgctg ataacggtca gttttaaaat
     1141 gcttttcagc ggccagacca ccgccagcct gcgctccccc tgggaagtca tcccctctga
     1201 tttttttata gtctattttt tggcgacgct gatcctgatt ctaattattt ttttcaacaa
     1261 aaaaattaaa ctttccctgc tgctgaccag ccttcacttc tttttatctt tatcagtcgg
     1321 cctgattatc tacaaaattg ggtttggttt tgacccgttc atccaccggg cggctgaaaa
     1381 atttattctt aacgccggcg ttatcacccc aaaaactttt tactatattg gccaatattc
     1441 actggtaact tttttagcca gactgcttca aatcccagtt gaactgatcg ataagctgat
     1501 cctgccttta ttaagcgcga tttatctgcc ggccatcgtt tatttctcgc tgtccaggct
     1561 tgattttttt aaaaataaac aagtcattgt ttttttgtca cttttatttt tagcggtccc
     1621 ctacgccggg ctgactgtca ccacccccca gagcctggct aacctttttt tggttttagt
     1681 cattttttta agtttaaacc tggttttaaa aagcgactat cagtccccgg tctggctttt
     1741 atttttactg gcttcggctt cattgctgat ccacccgctg gccggcattc cggctttcat
     1801 atttctgacg cttttgattt acctgacaaa atctgaagaa attcagctaa aatctttttt
     1861 taaaaaagca attttctggg aaattgtggc ggtcagcagc gtaattctgc cattgatttt
     1921 tttagggctg tcttttttgt caggtcagct gacaacagct ttcaaaacag atctgatcaa
     1981 cctgccgcga attgacctga ccagaaattt
//
LOCUS       DASWHZ010000166         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1414382, whole genome shotgun sequence.
ACCESSION   DASWHZ010000166 DASWHZ010000000
VERSION     DASWHZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414382"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..340)
                     /locus_tag="VGA49_03325"
     CDS             complement(14..340)
                     /locus_tag="VGA49_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664829.1"
                     /translation="MTEDKWLDLKAKIEDKFDILDQHKEELEDTPGTIEYIVFISPLG
                     KVKLERTIKPVVLDKKTTYTRRIGGQTAVDYVYSETETVDKIKALKWNDDQDDWEEME
                     AEGFLE"
     gene            complement(569..850)
                     /locus_tag="VGA49_03330"
     CDS             complement(569..850)
                     /locus_tag="VGA49_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX9664830.1"
                     /translation="MLEKYKKLLMDEKELYLRIKVIPSAARTELKEILADETFKIAVK
                     APPEKNKANQELIKFLGREFKCDKSSVKIISGQTDRLKLVKIKLWTLSA"
     gene            complement(882..1565)
                     /gene="uppS"
                     /locus_tag="VGA49_03335"
     CDS             complement(882..1565)
                     /gene="uppS"
                     /locus_tag="VGA49_03335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX9664831.1"
                     /translation="MTIKHLAIIMDGNRRWAKARGWPSLRGHQQGYDKVKRVGDWCLE
                     RGIKILTLYAFSTENWKRTEKEVKYLMGLLERALTVELNYFQDRDICLRVIGRKDELS
                     SKFQSIIQKAEEATKDNQAGILNLAINYGGRPEIIDAVKNIVRDKIKSEDINQELFSK
                     YVYTAGLPDPDLIIRTSGEQRLSGFLTWQSVYSEFYFIDKHWPDFSEKDLDEAIDWFD
                     KRERRFGGN"
     gene            complement(1641..1787)
                     /locus_tag="VGA49_03340"
     CDS             complement(1641..1787)
                     /locus_tag="VGA49_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664832.1"
                     /translation="MFNLHLILDDEGTTEEGGMAEGGAETPEEGGDAETSKEGGDAEA
                     EGGM"
     gene            complement(1869..2900)
                     /locus_tag="VGA49_03345"
     CDS             complement(1869..2900)
                     /locus_tag="VGA49_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX9664833.1"
                     /translation="MVYQNYRDARSRQPLSRHDRLRGVAKALGLSRTACKRLEWIIYW
                     ETRGKRKATLTARYFGIGRSTFHKWLSRFDETNLRTLESGCRAPKKRRKRNAVPLKDR
                     RVIELRKQYPYWGKMKLKVLYERCYNEEISSWYVQRVIEHYKLYFKKREKKTKTRHKT
                     QVKKRISQCQKEPQTGFLLHLDTIVLHLFGTKRYIITATDDHSKIAYARMYKSHASLA
                     AKDFFERLYFLFDSNVVNVHTDNGSEFHKHFDAGLKSLELPHYWSRVRTPKDNPSNER
                     FNRTLKEEFLYWGNFDKDPNIFNRKLTDWLVEYNSIRPHQNLNYLTPLEYAEKTQGLS
                     TMWSSSTRG"
     gene            complement(3077..3226)
                     /locus_tag="VGA49_03350"
     CDS             complement(3077..3226)
                     /locus_tag="VGA49_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664834.1"
                     /translation="MYECPSCGYTSDNPGDCPHCNVPLETVMDDSDDDMDDDMEDDDT
                     DDGEM"
     gene            complement(3373..3504)
                     /locus_tag="VGA49_03355"
     CDS             complement(3373..3504)
                     /locus_tag="VGA49_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664835.1"
                     /translation="MVNEKDQNIEERCRLCQTPLTSDNRSKNDPELCQYCGEDKAKE"
     gene            complement(3517..>3641)
                     /locus_tag="VGA49_03360"
     CDS             complement(3517..>3641)
                     /locus_tag="VGA49_03360"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX9664836.1"
                     /translation="HFWAKLIYYPALWFQRLTTAEPTDRDLELALAALKELLKS"
BASE COUNT          988 a          857 c          677 g         1119 t
ORIGIN      
        1 tattgactgt tgactattct aaaaacccct cggcttccat ttcttcccag tcgtcctgat
       61 cgtcattcca ttttaaagct ttgatcttat caactgtctc ggtttcagaa taaacataat
      121 caaccgccgt ctggccgcca attctccggg tataggttgt ttttttatca agaaccaccg
      181 gcttgatggt tctttccagt ttgactttac ccagcggact gataaaaaca atatactcaa
      241 ttgtgccggg ggtatcttcc agctcttctt tatgctggtc caaaatatca aatttatctt
      301 caatcttagc ttttaaatcg agccatttgt cttcagtcat aaaatttaga taaataataa
      361 caccaggcct aaagccacga caacagcggt aaatcctaag aatacataag taaatgcttg
      421 atgcatatta atacagtata gcaaaataag ttcaaagttc aaagtccaaa gttcaaagtt
      481 tcagccgaag gctgatccgc ctccggcgga caaagttcaa agtttcagcc aaaggctgca
      541 cgccatggcg gcatgccgtg gatccgcctc aggcggacaa agtccaaagt tttattttta
      601 caagctttaa tcggtcggtt tggccgctga taattttaac tgaagactta tcacacttaa
      661 attcccggcc aagaaattta attaattcct gattagcctt gtttttttca ggcggcgcct
      721 taacggcaat tttaaacgtt tcatcagcca gaatttcttt cagttcagtc ctggccgctg
      781 aaggaataac tttaattcta agataaagtt ccttttcgtc cattaataat tttttatatt
      841 tttccagcat aaaccaatta ccaactacca gtcactaact actaatttcc cccaaatcgt
      901 ctttcccttt tatcaaacca gtcaatcgcc tcgtccagat ctttttcact aaaatccggc
      961 cagtgcttgt caataaaata aaactcggaa taaaccgact gccaggttaa aaacccggac
     1021 aagcgctgtt cgccggaagt cctgataatc aggtcaggat caggcaggcc ggcggtataa
     1081 acatattttg aaaagagttc ctggttaata tcttcagatt ttattttatc tctgacaata
     1141 tttttaacgg cgtcaataat ttccggccgg ccgccgtagt taatcgctaa atttagaata
     1201 ccagcctggt tatctttggt ggcctcttcc gctttttgaa taatagactg gaattttgaa
     1261 gacagctcgt ctttccggcc gattacccgc aaacaaatat ctctgtcctg aaaataattc
     1321 agctcaacag ttaaagctct ttccagcaag cccattaaat atttaacttc cttttcggtt
     1381 cgcttccagt tttcggttga aaaagcgtag agagtcaaaa ttttaatccc ccgctccaaa
     1441 caccagtcac ccacccgctt aaccttgtca tagccctgct gatggcccct gagcgacggc
     1501 cagccacggg ctttggccca gcggcggttg ccgtccataa tgatcgctaa atgtttgatt
     1561 gtcataattt gattataaac aaaaattgct cgttaaagca atttttgtcg tatgataaaa
     1621 cttagacgat gaaaacggat ttacatgccg ccttctgctt cagcgtcgcc gccttcttta
     1681 gaagtttcag cgtcgccgcc ttcttcagga gtttcagcgc cgccttctgc cataccacct
     1741 tcttcggtag taccttcatc atccaaaatt aaatgtaaat taaacatgtt tttttcctta
     1801 attaattcct taaataaaaa atcgaccttt aaggtattaa ttcataataa actattgtca
     1861 aaaagctgtc aaccccgtgt actggatgac cacatcgtag acaacccctg agttttttcc
     1921 gcgtattcca gaggtgtcag ataattgagg ttttgatgag gacgaataga gttgtactca
     1981 acaagccaat cagttaactt gcgattaaaa atgttcggat ctttgtcaaa gtttccccag
     2041 taaaggaatt cttctttcag cgtacgattg aaacgctcat tcgatggatt gtctttgggc
     2101 gttcggaccc gactccagta atgcggaagc tctaaacttt ttaaaccggc atcaaaatgt
     2161 ttgtgaaatt cactgccatt gtccgtatgg acattcacca cattactgtc aaaaagaaag
     2221 tacagacgtt caaaaaaatc tttcgcggcc aggctggcat gtgatttata catgcgagca
     2281 tacgcaattt tagaatgatc atccgttgcc gtaatgatgt atcgtttcgt accaaacaga
     2341 tgtaaaacaa tggtatccaa gtgaagaaga aatcctgttt gaggttcttt ttgacactga
     2401 ctgattcttt ttttcacttg tgttttatgc cttgttttgg tctttttctc tcgtttctta
     2461 aaataaagct tgtagtgctc gatgaccctt tggacatacc aggaacttat ctcctcatta
     2521 tagcaacgtt catagagaac tttgagtttc attttacccc agtaggggta ttgctttcgt
     2581 aattcgatga cccgtcggtc ttttaatgga acggcattgc gttttcgtct tttcttgggg
     2641 gcgcgacagc cgctttctaa ggttcgcaaa tttgtttcat caaatcgaga caaccattta
     2701 tggaaagtac ttcgcccgat gccaaaatat ctggccgtta gggtcgcctt ccgcttacca
     2761 cgagtttccc aatagatgat ccattccaga cgcttacagg cggtcctgga tagacccaaa
     2821 gcctttgcga cgccgcgaag ccggtcatgc cgcgatagtg gctgcctgga tctggcgtca
     2881 cgataattct gatacaccat aatattgagg ttactaagag aatttctctt aatttacctc
     2941 aattatctat ttttgtactg tctacgatgt atctcatctt tttcaccccg ctcttcgcga
     3001 agggcggggt aaaccccgct aaagcaaaaa gtgccttaat aattaaaagg cacttcttgt
     3061 ccaggtcgcc ggataattac atttctccgt catccgtgtc atcatcttcc atatcgtcat
     3121 ccatatcatc atcgctatca tccatgactg tctccagagg cacgttacag tgcgggcagt
     3181 caccagggtt gtcgctggtg tagccgcatg atggacattc gtacatattg tgttgataat
     3241 aattaaatta acgctatcaa cgacctttaa ttattttatt aacttaataa tatgaaacgg
     3301 cctaacctct gtcaaccccg cccttcgcga agggcggggt caatcccatc actcgcgaag
     3361 ggcgggttaa atctattctt tcgccttatc ctcgccgcaa tactggcaca actccggatc
     3421 atttttagag cgattatccg aagtcagcgg cgtctgacac agccggcagc gctcttctat
     3481 attttgatct ttttcattaa ccataattta aaaaatttaa cttttaagca attctttcag
     3541 ggcagccagg gccaattcca gatcccggtc ggtcggctcg gccgtggtta aacgctgaaa
     3601 ccataaagcc ggataataaa ttaatttggc ccagaaatga t
//
LOCUS       DASWHZ010000167         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1419560, whole genome shotgun sequence.
ACCESSION   DASWHZ010000167 DASWHZ010000000
VERSION     DASWHZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419560"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /gene="uvrA"
                     /locus_tag="VGA49_03365"
     CDS             <1..1274
                     /gene="uvrA"
                     /locus_tag="VGA49_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9664837.1"
                     /translation="PSIGLHRRDNQKLIKTLADLRDLGNTVVVVEHDSQTILSADHLI
                     DLGPGAGIHGGKIVAEGSPKDIIKNKNSLTGQYLSGRKKIEIPKKLRSPRDFIELVGA
                     SQHNLKNINVKFPLSVLCCVTGVSGSGKSTLVNETLYKGLAKEIGRARIIPGQYQKIN
                     KLDKVDKVIAIDQSPIGRTPRSNPATYIKVFDYVRELLSQTKEARARGYQPGRFSFNV
                     QGGRCEPCRGDGLIKIEMHFLPDIYVECEKCKGQRYNRETLEVTYKDKNIADILNMTV
                     EEALEFFQNIPRIEKKLKTLADVGLGYVKLGQSSTTLSGGEAQRIKLTAELSKRDTGQ
                     TVYILDEPTTGLHFEDIKKLLAVLNRLVDKGNTVIVIEHNLDVIKTADYIIDLGPEGG
                     DAGGLVIAEGSPADIIKNKASFTGKFLKTII"
     gene            1274..>2381
                     /gene="uvrC"
                     /locus_tag="VGA49_03370"
     CDS             1274..>2381
                     /gene="uvrC"
                     /locus_tag="VGA49_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9664838.1"
                     /translation="MSLSKQKLNNLPNKPGCYLFKNSTGAILYIGKAKNIKKRVKSYF
                     QKKSLDYKTTGLVSRIADIDLIVTDNELEALLLESKLIRENKPKYNIDLKDSVRYAYL
                     KITDEKFPRIITTRQVDKEGGYFGPYGDANSRETASYYAHSLFKIRTCDKLPKRPCLQ
                     YYIKRCDAPCVGNISEEAYNKNVRNAVDFLKGKTGSLKRKLKNQMKKYSDSLEFEKAK
                     IIREQLKGLEKLKQLQKQKVALIKAYNEDLINYIKLGEKIYINLFTIDKGTISGKKEF
                     IFDDRPEILEDFIRQYYAGNDIPDELILPVKIDDQALIVKYLSRLKKRKVTINIPRTG
                     VRKKLLNLVKKNIEQAIKQQDPALVELQEKLNLVN"
BASE COUNT          791 a          461 c          525 g          604 t
ORIGIN      
        1 aaccgtcaat cggcctccat cggcgggata atcaaaaact gattaaaacc ctggctgatt
       61 taagagattt gggcaatacc gtggtggtgg tggagcatga cagccagaca attttgagcg
      121 ccgatcatct gattgatctg ggtccgggcg ccggcatcca cggcggtaaa atagtggctg
      181 aaggctcacc caaagatatt ataaaaaata aaaattcttt aaccggccaa tatttgtcag
      241 gccggaaaaa aattgaaatt cccaaaaaat tacggtcacc ccgggatttt attgaactgg
      301 tcggcgccag tcagcataat ctgaaaaata tcaatgttaa atttccatta agtgttttat
      361 gctgcgtcac cggcgtcagc ggcagcggca agagcacttt agtcaatgaa acattatata
      421 aagggctggc caaagaaatc ggccgggccc ggattattcc cggccagtat cagaaaatta
      481 acaagctgga caaggttgat aaagtcattg ccattgatca gtcgccaatc ggccggacgc
      541 cccggtccaa tccggcgact tatattaagg tctttgatta tgtcagggaa ctactcagcc
      601 agactaagga agccagagcc cggggctatc agcccggtcg gtttagtttt aatgttcagg
      661 gcggccggtg tgagccttgc cggggcgatg gtttaattaa aattgaaatg cattttttgc
      721 ctgacattta tgttgagtgt gaaaaatgca aaggtcaaag atataatcgt gaaactctgg
      781 aagttaccta caaagacaaa aacatcgccg atatactaaa tatgacagtt gaggaagctt
      841 tggaattttt tcaaaatatt ccgagaattg agaaaaaact aaaaactttg gccgatgtcg
      901 ggctgggtta tgttaagctt ggtcagagtt caaccacttt atcgggtggc gaagcccaaa
      961 gaattaaatt aacggccgaa ctgtccaaaa gagacaccgg tcagactgtt tatatcctgg
     1021 acgagccgac aaccggtctg cattttgaag atattaaaaa actgctggcg gttcttaatc
     1081 ggctggttga taaaggcaac acagttattg tgattgagca taatctggat gtcattaaaa
     1141 cagccgatta tattattgac ctggggccgg aagggggcga tgccgggggc ctggttatcg
     1201 cggaagggag cccggcggac attattaaga ataaagcatc attcaccggt aaatttttaa
     1261 aaactataat ttgatgagtc tttcaaaaca aaaattaaat aacctgccta ataagcccgg
     1321 ctgttatcta ttcaaaaact caaccggagc cattctttac attggtaaag ctaaaaatat
     1381 aaaaaaacgg gttaagtctt attttcagaa aaaaagttta gactacaaaa caaccgggct
     1441 ggtttctcgt atcgcggata ttgatttgat tgtgactgac aatgaactgg aagctttgtt
     1501 actggaatca aaattaatca gagaaaacaa acctaaatac aacattgatc tgaaagattc
     1561 agttcgttac gcctatttaa aaattactga cgaaaaattc cccagaatta ttaccaccag
     1621 gcaggttgac aaagaaggcg ggtattttgg gccctacggc gacgccaaca gccgcgagac
     1681 cgcctcatat tacgctcaca gcctgtttaa gatcagaacc tgcgataaac ttcccaaaag
     1741 accctgtctg caatactaca tcaaacgctg cgacgccccc tgcgtcggca atatttcaga
     1801 agaggcttac aataaaaacg tcagaaacgc cgttgatttt ctcaagggca aaacaggatc
     1861 gcttaagagg aaacttaaaa atcagatgaa aaaatattct gacagcctgg aatttgaaaa
     1921 agccaaaatc atccgggaac agctcaaagg gctggaaaaa ttaaaacagc ttcaaaagca
     1981 aaaagtggct ttgatcaaag cttataatga agatttgatt aattatatta agttgggaga
     2041 gaaaatttat attaatcttt tcacgattga taaaggaacc atctctggca aaaaagaatt
     2101 tatatttgac gaccggcctg aaattttaga agattttatc agacaatatt acgccggcaa
     2161 cgacattccg gatgaattaa ttttgccggt taaaattgat gatcaggctt taattgtcaa
     2221 atatctttcc aggctgaaga aaagaaaagt tacaattaat attccccgga caggcgtcag
     2281 gaaaaaactt ttgaatttgg ttaaaaagaa tattgagcag gcgattaaac agcaggatcc
     2341 ggccctggtt gagcttcagg aaaaactgaa tttggtcaat g
//
LOCUS       DASWHZ010000168         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_306085, whole genome shotgun sequence.
ACCESSION   DASWHZ010000168 DASWHZ010000000
VERSION     DASWHZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306085"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..839)
                     /locus_tag="VGA49_03375"
     CDS             complement(30..839)
                     /locus_tag="VGA49_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9664839.1"
                     /translation="MFQWIRFGIVFVLFFSGCQATQPAAEPAQQKKARPATGGQSVSL
                     PADSVLAEAIFEEAEDENLAGVRNAVCALYIGGEFRGNGVILNHRLIVTSVNVISGIL
                     RIVENPTGELKVQRLELRIRRPNRLALAEAELLRVSLATDLVIISARLEAGFNPPIVL
                     TDQFQTGANCRIIGYNSSDLTMGQTKAVIGRQIGRQSVRDELLDGAAVCFWEVQSVEE
                     IHSYQHGGAVVDEQGRLIGILGIAGRRVDQQGKMIYVGNVITNIETVFGEK"
     gene            complement(917..>2460)
                     /locus_tag="VGA49_03380"
     CDS             complement(917..>2460)
                     /locus_tag="VGA49_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX9664840.1"
                     /translation="LAGMTGTALTEAEEFAKIYNLDVTVIPTNKPLIRQELSDRIYKS
                     EMGKFQAVVREIKERHEKGQPVLVGTISIQKNELLGQLLDRSGVPHQALNAKNHEKEA
                     SIIAQAGRTGAVTIATNMAGRGVDIILGGNPASEAAAGQVRQLGGLHVIGTERHDSRR
                     IDNQLRGRAGRQGDPGSSQFYVSLEDDLMRIFGSDRIKNLMTALKVPEDMPIENKIVT
                     RSLETAQKKVEGHNFDIRKHVVEYDDVLNKHREVIYKKRREILELEQSGLSVKDKILE
                     MVKNEIEQVVGFHTQSDDQSAWDLKEVLEVIGTIFQMTDDEKSKLNDIYQPAASQTDP
                     TDSRIKIIEYLMSLARDKYQALEGKVKETVGNDETFRQIEKGILLRSYDQLWVEHLEA
                     ITHLRRGIGLRGYGQRDPLVEYKKEAFKMFNILLASIQKQVVYSVYKISVGIQMAPSL
                     MQRSGIRFSAPAKTGQTQSAFAQNDGNGQQKQVAVQDDKFKNVGRNDPCPCGSGKKFK
                     KCHGA"
BASE COUNT          585 a          683 c          526 g          666 t
ORIGIN      
        1 cgtgatgaca gcgtgactgt ggccctcctc tacttttcgc caaaaaccgt ttcaatgttg
       61 gtaatgacat tgccaacata aatcattttg ccctgctggt ccactcgccg gccggcaatc
      121 cccaaaatgc caatcagccg cccctgctcg tcaacaaccg ccccgccgtg ctgataagaa
      181 tggatctctt ccactgactg aacttcccaa aaacaaacag ccgctccatc cagcaattcg
      241 tcccggactg actgccggcc gatctgccgg ccaatcaccg ctttggtctg ccccatggtc
      301 agatcgctgg aattgtagcc gatgatccgg caattggcgc ctgtctgaaa ctgatccgtc
      361 aaaacgattg gcggattgaa tccggcctca agcctggcgc tgatgatcac caaatcagtc
      421 gccagtgaca cccgaaggag ctcggcttca gccagcgcca gccgattggg ccgcctgatc
      481 cgcagttcca gcctttggac tttgagttcg ccggtcgggt tctcaacaat cctcaaaatg
      541 ccgctgatga cattgactga agtcacgatc agtcggtgat tcagaataac gccgttgccc
      601 ctgaactcgc ctccaatgta aagagcgcaa actgcgtttc tgactccggc cagattttca
      661 tcttctgcct cttcaaagat cgcctcggcc aaaactgaat cagccggcag actgaccgat
      721 tgcccgccgg tcgccggcct ggccttcttt tgctgagccg gttcagcggc tggctgggtc
      781 gcctggcagc ctgaaaagaa caaaacaaaa actatcccga atcggatcca ttgaaacaac
      841 ttcttacctc ctgattaagg gttacgggaa gttttaattt atcaaaagaa aaaattactg
      901 tcaatttgtt acaagactaa gctccgtggc attttttaaa tttcttgccg ctgccgcagg
      961 gacagggatc attcctcccg acatttttga atttgtcatc ctgaacagca acttgttttt
     1021 gctgaccatt accgtcattt tgagcaaaag ctgactgggt ctgaccggtc ttggccggag
     1081 cggaaaacct gatcccgctt ctctgcatca atgacggcgc catttggatg cccacgctga
     1141 ttttataaac gctgtaaaca acctgctttt gaatactggc cagcaaaatg ttaaacattt
     1201 taaaagcctc ttttttatat tcaaccagcg gatcccgctg accatagccc cggaggccaa
     1261 tcccccgccg caaatgagtt atcgcttcca gatgctccac ccacaactga tcatagcttc
     1321 ttaataaaat acctttttca atttggcgaa aagtttcgtc attgccaact gtttctttaa
     1381 ccttgccttc taaagcctgg tatttatccc gggctaaact catcaaatat tcaataattt
     1441 taatccggct gtcagtcgga tcagtctgac tggcggccgg ctgataaata tcgtttaatt
     1501 ttgacttttc gtcatcagtc atctgaaaaa tcgtgccgat aacctccaaa acttctttca
     1561 gatcccaggc gctttgatcg tcagactggg tatgaaaacc aacaacctgt tcaatttcat
     1621 ttttcaccat ttccagaatc ttatctttga cagaaagccc ggactgttcc agctccaaaa
     1681 tttcccgtct ttttttataa ataacttccc gatgcttatt taaaacatcg tcatactcca
     1741 cgacgtgctt tctgatgtca aaattgtggc cttctacttt tttctgggcg gtttccagcg
     1801 accgggtgac aatcttattt tcaatcggca tgtcttccgg cactttcaga gcggtcatta
     1861 aatttttgat ccggtccgaa ccaaaaattc tcatcagatc atcttccagg gaaacataaa
     1921 actgggatga accaggatcg ccctgacggc cggcccgacc ccgaagctga ttgtcaatcc
     1981 gccgggaatc atgacgttca gtgccaataa cgtgaagccc tccaagttgg cggacttgtc
     2041 ccgccgctgc ctcggaggcg gggttgccgc ccaaaataat atcaacgccc cggccggcca
     2101 tattggtggc aatggtcacg gcgccggtcc ggccagcctg cgcaataatg ctggcttctt
     2161 tttcatggtt tttggcgttt aatgcctgat gggggacgcc tgaacggtcc aacagctgac
     2221 ccaaaagttc atttttctga atggaaattg tcccaaccaa aaccggctga cctttttcat
     2281 gccgttcttt aatttctctg acaactgcct gaaacttgcc catttctgat ttgtaaattc
     2341 ggtcagacag ttcttgtctg atcagaggct tgttggtcgg gataacggtt acgtccagat
     2401 tatatatttt ggcgaattct tccgcttcag tcagggcggt gccggtcatg ccggccagct
//
LOCUS       DASWHZ010000169         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3611764, whole genome shotgun sequence.
ACCESSION   DASWHZ010000169 DASWHZ010000000
VERSION     DASWHZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3611764"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1389)
                     /locus_tag="VGA49_03385"
     CDS             complement(4..1389)
                     /locus_tag="VGA49_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9664841.1"
                     /translation="MKEIDPSIFRAYDIRGIYPDTLNEDLVYRIAQSYLKIIKPAGQD
                     IVLGGDVRESSPQLKKAAADGLLDSGYNVIDIGTVSTDMFYFAVANYGYAGGLSITAS
                     HNPREYNGIKMVREKAQSVSGDSGIMEIRDDILAVSQKIESVKKGQVTKKDIINDYSQ
                     LCLSFIDPFKIKKFKIVMNPNFGLAGQVVQEMLTGLPIEIVPLNFEPDGSFPKGRPDP
                     LIPENREETIELVKSSQADFGVAWDADADRVFFLDESGGFIDGYFMTAILAKIMLAKQ
                     PGAKIISDPRLTWAVRDTVEAEGGRVLVNKVGHSFIKERMVKEDAFFGGENSAHYYFK
                     FENGAYYDNGMIPFLLILEKLSVDNLKLSELAQPFRKKYFISGEINRAVKDKEKVLRQ
                     AEEKYSDGQTEKIDGLSVEYANPAGRASGASWRFNLRTSNTEPLIRLNVEATSQSLME
                     SKRDEILKIIK"
     gene            complement(1516..>2667)
                     /locus_tag="VGA49_03390"
     CDS             complement(1516..>2667)
                     /locus_tag="VGA49_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664842.1"
                     /translation="IKIILMASLMAAVLLAGPAVAGTRINQTNGDLLIAKNNYYTSNL
                     YKFGRNIVVEGTLEKDLVVVGDQVVVSGSIGGDVIVMANQVRINGPVKGNVRVFADTI
                     QLDSSIGKNVTLFAENIFLLKNTEIGWDAVMAAKKIRAQAEIGGDLLAASKDIMVSGR
                     VGGNFNLYLNKQGQLTLNSGTIIGRDLNYISQNKLAIPEKTVGGLVNYYPGQSSYAGS
                     YLKSFYAIKIIFSLLGLWLVGLILVAVFKKKLVPVTNLMAEKVGASLLAGAIFVILLP
                     VVVMILLATLIGLPLALIIACLGLLLIYIGEFFVGLLIGQYVWSCFDKKKISKAPLMP
                     AMFLGVFLFFVLISIPVIGWLVKFVGMIWGSGALILGFKERHKARGTRQ"
BASE COUNT          746 a          665 c          520 g          736 t
ORIGIN      
        1 caactattta attattttca aaatttcatc acgcttagat tccatcagtg attgagaagt
       61 cgcctcaaca tttagccgaa tcagcggttc ggtgttagaa gttcttaaat taaaccgcca
      121 gcttgccccg ctggccctgc cggcggggtt ggcgtactca accgataagc cgtcaatttt
      181 ttcagtctgg ccgtcgctat atttttcttc agcttgtcgc aagacttttt ctttatcttt
      241 aactgcgcga ttgatttctc cggagataaa atattttttt ctgaatggtt gagccagctc
      301 ggacagttta agattatcaa ccgacagttt ctctaatatt aaaagaaaag ggatcatccc
      361 attgtcatag taagcgccat tttcaaattt aaaataataa tgggcgctgt tttccccgcc
      421 aaaaaaagcg tcttctttga ccattctttc tttaataaat gaatgcccga ctttattgac
      481 caaaacccgg ccgccttcag cttcaactgt atctctgacc gcccaggtta aacgaggatc
      541 tgaaataatt tttgctcccg gctgtttagc cagcattatt ttagccagga tagccgtcat
      601 aaaatagccg tcaataaatc ccccgctttc atccaaaaag aaaacccggt cggcgtccgc
      661 gtcccaggcc actccaaaat cagcctgact gcttttaacc agctcaattg tctcctctcg
      721 attttcagga attaagggat cgggccggcc tttgggaaat gagccgtccg gttcaaaatt
      781 taaaggaaca atttctattg gcagccctgt taacatttcc tgaacgacct gtccggccag
      841 accaaaattg ggattcatca cgattttaaa tttttttatt ttaaacggat caatgaatga
      901 caaacacagc tgactataat catttataat atcctttttt gtaacttggc cttttttaac
      961 cgattcaatt ttttgactaa cggctaaaat atcatcccta atttccataa tcccggaatc
     1021 gccggagact gactgggcct tctcccggac cattttgatc ccgttatatt cccggggatt
     1081 atgggaagcg gtgatgctta aaccgccggc gtagccgtaa ttggcgaccg cgaaataaaa
     1141 catgtcggtt gaaaccgtcc cgatgtcaat cacattatag ccgctgtcca gtaacccgtc
     1201 agcggccgct ttcttcagtt gaggtgaaga ttcccggaca tcgccgccta agactatatc
     1261 ttggcccgcg ggctttataa ttttcaaata actctgggca atccggtaaa cgaggtcttc
     1321 attgagagtg tcaggataaa tgccccgaat atcgtaggcc cggaaaattg agggatcaat
     1381 ttctttcata caaattttaa ggcaataggc acaaggcaca agggttggtg tcgcttcgcc
     1441 agtctattgc ctggcttcgc gacatattat tagaaaatct attgcctgat ccccatctaa
     1501 taaattattc ctaccctatt gccttgtgcc tcgtgccttg tgcctttctt taaagcccag
     1561 tattaacgct ccgctgcccc aaatcatccc aacaaacttt accagccagc cgatgaccgg
     1621 gattgaaatt aatacaaaaa acagaaaaac tcccaaaaac atcgccggca ttaaaggggc
     1681 tttggaaatt ttctttttgt caaaacagct ccagacatac tggccaatca gcaaaccaac
     1741 aaaaaattct ccgatataaa tcagcaataa accaaggcag gcgataatta aagccaacgg
     1801 caagccgatc agcgtcgcca gtaaaatcat caccacgacc ggtagtaaaa taacaaaaat
     1861 ggctccggcc agtaaactgg cgccaacttt ttcagccatt aaattcgtca cgggaaccag
     1921 tttctttttg aaaacagcga ccaaaatcag accgaccagc cataagccca gcagactgaa
     1981 aatgattttg atagcgtaaa aagattttag ataactgccg gcgtaactgc tctggcccgg
     2041 ataataattg accagaccgc cgactgtttt ttccggaatc gccagcttgt tttgggagat
     2101 atagttcaaa tcgcggccga taatggttcc ggaattcaga gtcagctggc cttgtttatt
     2161 cagatataaa ttaaaattgc cgccgacccg gccgctgacc ataatatctt tggaagcggc
     2221 cagcaggtca ccgccgattt cggcctgggc tctgattttt ttggcggcca taaccgcgtc
     2281 ccagccaatt tcggtatttt ttaaaagaaa aatattttca gcgaacaaag taacgttttt
     2341 gccaatactg gaatcaagct gaatggtgtc ggcaaaaacc cggacattcc ctttaaccgg
     2401 tccgttgatc ctgacctgat tggccatcac aatcacgtcg ccgccaatac tgccgctgac
     2461 aaccacctga tcgccgacaa ccactaaatc tttttccaaa gtcccttcga caacaatatt
     2521 ccggccgaat ttatataaat tgctggtata ataattattt ttggcgatta aaagatcacc
     2581 gttggtctga ttaatccggg ttccggccac ggccggcccg gccagtaaaa ccgccgccat
     2641 caggctggcc ataagaatta tttttat
//
LOCUS       DASWHZ010000170         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_689078, whole genome shotgun sequence.
ACCESSION   DASWHZ010000170 DASWHZ010000000
VERSION     DASWHZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689078"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..537
                     /locus_tag="VGA49_03395"
     CDS             124..537
                     /locus_tag="VGA49_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664843.1"
                     /translation="MINEKARQNRERYELFLGLTRGPSLIIPVSAILLLLLSYRFLNL
                     DQITAFIAIGVYEFIALLIAGLIVSRKVNDRKILTSAGAFTGLLTGVTIGFFKLLWYH
                     KFWTVINLIAEPAVTAVLGFIAYVVVANIKRIKSE"
     gene            654..1055
                     /locus_tag="VGA49_03400"
     CDS             654..1055
                     /locus_tag="VGA49_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEX9664844.1"
                     /translation="MVRGDNLIYPDLSYKLMGISFKVYNSMGFGFREKYYQRAFQKEL
                     IKNGIAHQIEKEIKLRYDNEVIGKYFLDFVIEDKIILELKVVSFIKNIHIKQVLEYLN
                     ATNLKLAILIYFTKTGVIYKRVVNPNLKLHQ"
     gene            1120..>1558
                     /locus_tag="VGA49_03405"
     CDS             1120..>1558
                     /locus_tag="VGA49_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9664845.1"
                     /translation="MISFKKRTKIICTIGPVSADQETLEKLIKNGMNVARLNFSHGSY
                     DSHAGLIKNIRAAAKKLNQPVAILQDLQGPRIRIGEVAGEGLKTKKGDQVALVAPKLK
                     NSARGGSTSPASLREAGRAGGKKIKNYSVVPIQYENLYKDVKKG"
BASE COUNT          495 a          270 c          311 g          482 t
ORIGIN      
        1 tgtctaattt ttaatttcta atcaatgtct aatgacaaat gactaaattt ttttgagatt
       61 agtcatttaa ggattgcggc ttgattagga attaggtcaa ttagaaatta gaaatttgac
      121 actatgatta atgaaaaagc cagacaaaat agagaacgtt atgaactttt cctgggccta
      181 acccgtggtc caagtctaat cattccggtt tccgccattt tattattgtt attgagttat
      241 cgttttttaa atcttgacca aataaccgct ttcatcgcca tcggagttta tgagtttatc
      301 gctctgctga tcgccggact gatagttagc cgaaaagtta atgatcgaaa aattttaact
      361 tcagccggcg ccttcaccgg tttactgacc ggcgtcacca tcggcttttt taaactgttg
      421 tggtatcaca agttctggac ggtgataaat cttattgccg aaccggccgt aaccgctgtt
      481 ttgggattta tcgcgtatgt tgtggtcgct aatattaagc gaatcaaaag cgaataagat
      541 aacgaataag cgaatttaat ctaataagag cgaattggcg gttgctaatt tttgaattta
      601 agcctaatat ttaactagat tgtccacccc gataagatca ttaatcaaaa tttatggtta
      661 ggggtgacaa tttaatttat ccagatttaa gttataaact gatgggcatc tcctttaagg
      721 tttataactc aatgggtttt ggttttcggg agaaatatta tcagcgcgct ttccaaaagg
      781 aattaattaa aaatggcatt gcccatcaaa ttgagaagga aataaagttg cggtatgata
      841 atgaagttat tggaaaatat tttttagact tcgttattga agataagatt attttagaat
      901 taaaagtggt ttcttttatt aaaaatattc atatcaaaca ggttttagaa tatctgaacg
      961 cgacaaattt gaaattagcg attctgatat attttaccaa gacaggagtt atttataaac
     1021 gtgttgtaaa tccaaactta aagctccatc agtaatgtta tccccaaatc cgtttatatt
     1081 cgctaataat ttgttttata attcgcttat gattagctta tgattagctt taaaaaacgc
     1141 accaagatta tttgcaccat cggcccagtt tcagctgatc aggaaacact ggaaaaattg
     1201 ataaagaatg gcatgaatgt cgcccgtctc aacttttctc atggcagtta tgacagccac
     1261 gccgggttaa ttaaaaatat ccgggcggcc gccaaaaaac tgaatcagcc ggtggctatt
     1321 ttacaagatt tacaagggcc aagaatcagg attggcgaag tggctggcga ggggctgaaa
     1381 actaagaaag gcgatcaggt cgctttggtc gcgccaaaac taaaaaattc cgcccgaggc
     1441 ggatccacct ccccggcctc gctccgcgaa gcggggcggg ctggtggaaa aaaaatcaaa
     1501 aattattcag tggtcccgat tcaatatgaa aatctctata aagacgttaa aaaaggat
//
LOCUS       DASWHZ010000171         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1079322, whole genome shotgun sequence.
ACCESSION   DASWHZ010000171 DASWHZ010000000
VERSION     DASWHZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079322"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VGA49_03410"
     CDS             <1..1575
                     /locus_tag="VGA49_03410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9664846.1"
                     /translation="FDHSRIILNSPDETAHYFFSRIFAEETTLQFFEPLNFLSNNGLK
                     PRSVKVINGYLTPGGFIGLNIIYGSLAKIFGVKAVYFFTPFMAALAVLFFYGLIKIIF
                     DQRIAFVSALLLLIQPAFWYYASRGLLPNVLLVSLLIIGFYFLLKAFEISKIKVYWQW
                     LFAGLAGGLISLALTVRPTEYLWVGLILITLFFGYIKRLSFIKIFLFLVAAFLAFCPV
                     FYHNTIVYGQPYLTGYSLSDTKYALQPDLEERPILTGEFMAAPPLLNQGIWKFVYPFK
                     FHPNNIIRNFVNFYLKFFWWLNILAAVGLIIYLVKYGLEKSKIIYLALLSTISAWLIV
                     YYGTWLFFDNITPDRITIGTSYVRYWLPSFILSLPLISLVILYVLKVRRLFFKILLGV
                     VLGAAILLMNARITLWQGEESLRANLENLLDYQRKAENVFQLTPPEAVIITNRTDKLF
                     FPERRVMFYVDNDFTIINHLNRLLDEAPIYWFTMMPNDDIVYVNSLLEKFGLVLTNPE
                     DIIGEERLFEVQRIID"
     gene            1734..>2188
                     /locus_tag="VGA49_03415"
     CDS             1734..>2188
                     /locus_tag="VGA49_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664847.1"
                     /translation="MPYKTIKIILILIPIVGFVWLVNKNLAFSGQLTVKYDFSKKSAF
                     ISELYPNSRVSSVQRDEQGEYFQALTSHPVYFDLKMPRTFNQAEIIIKYKNSDNPIFE
                     IGAKAGPAEDQFDLKPVENQILDRLIEDKTGWSAIGHQETVLIQKNYAG"
BASE COUNT          581 a          382 c          455 g          770 t
ORIGIN      
        1 tttgatcatt cccgaattat tctgaattca cctgatgaaa ccgcccatta tttttttagc
       61 cgaatttttg ctgaagaaac aactttacag ttttttgaac cactgaattt tttgtcaaat
      121 aacggactga aaccaaggag cgttaaagtg attaacggtt atctaacacc gggcgggttc
      181 attggattga atattattta cggcagcctg gccaaaattt ttggggttaa agcggtttat
      241 ttctttaccc cgtttatggc cgctttggct gttttatttt tttacggttt aataaaaatt
      301 atttttgatc aaagaattgc ttttgtctcg gccctgctgc tgttgattca gccggctttt
      361 tggtactacg ccagccgggg attattaccc aatgttttat tggtcagtct gctgatcatt
      421 ggtttttatt ttttgcttaa agcctttgaa atcagcaaaa taaaagttta ttggcagtgg
      481 ctgtttgccg gtttagccgg cggactgatc agcctggctt taacagtcag gccgacagaa
      541 tatctctggg ttggtttgat cctgattact ttgttttttg gttatattaa aagattaagt
      601 tttattaaaa tatttttatt tctggtggcc gcctttctgg ccttctgtcc ggtgttttac
      661 cacaacacga ttgtttacgg ccagccatat ctgaccggtt attctttaag cgacactaaa
      721 tacgctttac agcctgattt ggaggagcgg ccaattttaa ccggcgaatt catggccgct
      781 ccgcctttgc tcaatcaggg gatttggaag tttgtttatc catttaaatt tcatcccaat
      841 aatatcatca gaaattttgt taatttttat cttaaatttt tctggtggct gaatattctg
      901 gcggctgttg gtctgattat ttatttagtt aaatatgggc tggaaaaatc aaaaataatc
      961 tatctggctt tgctgtccac tatttccgcc tggctgattg tttattatgg cacctggctg
     1021 ttttttgaca atattacccc cgaccggatc actattggca cttcttatgt tcgatactgg
     1081 ctgccgtcgt ttattttaag tctgcctctg atcagcctgg taattttata tgttttaaaa
     1141 gtcaggcggc tgttttttaa aatattactc ggggttgttt tgggggcggc gattttattg
     1201 atgaacgccc ggataacatt atggcagggt gaagaaagcc ttagggcgaa tttggaaaac
     1261 ttgcttgatt atcagcgtaa agctgaaaat gtttttcagc taaccccgcc tgaagccgtg
     1321 attatcacca accggactga caagcttttt ttcccggaac gtcgcgtaat gttttatgtt
     1381 gacaacgatt ttacaattat taatcattta aacaggttat tggatgaagc gccgatttat
     1441 tggtttacga tgatgcctaa cgatgatata gtttatgtta attctttact ggaaaagttc
     1501 gggctagttt taaccaatcc ggaggatata attggagagg aacggctgtt tgaggttcaa
     1561 aggatcattg attaaattct aaataataaa ttataatgtt ccaaaaaatc aacattaatt
     1621 gtttaaaatt tataacatta taatttatga tttatttagt atttctcccg actttgtcga
     1681 ggatccgcga tggctttgcc atcgggagaa tttaggattt agaatttcta tttatgcctt
     1741 ataaaacaat taaaataatt ttaatcttaa taccaattgt cgggtttgtt tggctggtta
     1801 acaagaattt ggcttttagc ggtcagttga cagttaaata tgattttagt aaaaagtcag
     1861 ctttcatttc agaactgtat cccaacagcc gggtcagttc agtacagcgg gatgaacagg
     1921 gagaatattt tcaggcttta accagccacc cggtttattt tgatttgaaa atgccgcgga
     1981 cttttaacca ggcggaaatt ataattaaat ataaaaattc agataatcca atttttgaaa
     2041 ttggcgccaa agccggtccg gcggaagatc agtttgatct gaagccggtt gaaaatcaga
     2101 ttttggaccg gttaattgaa gataagaccg gctggtcggc gattggtcat caggagaccg
     2161 ttttaatcca gaagaactac gccggtca
//
LOCUS       DASWHZ010000172         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3286666, whole genome shotgun sequence.
ACCESSION   DASWHZ010000172 DASWHZ010000000
VERSION     DASWHZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3286666"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1056)
                     /locus_tag="VGA49_03420"
     CDS             complement(46..1056)
                     /locus_tag="VGA49_03420"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9664848.1"
                     /translation="MPEPIFEKKNILVTGGAGFIGSHLCEDLLKSSKVICVDNFVSGS
                     QGNIDHLLRFPDFIFLKHDINQPLELEKFPELARFRIEFQGIQEVYNLACPTSPKNFD
                     QYKIDTIKANTVGLMNMLDLAIKYKAKFLHGSSSVVYGGRTDQQTIFKEDYLGILNQM
                     SPRACYDSGKQYAEAIVNTYEDIHNLDAKIARIFRTYGPRNRLNDGEMMPDFIVNALD
                     NKDLVIYGDDNFRTTLSYVTDLVDGLVRLMAAPADIGPVNLGSDVDLKLSDVARKIID
                     MTGSSSNVVFKEPLVFMTPLGIPDISRARDQLAWLPVFSLEKGLEKTIEYAKAEKVRK
                     TI"
     gene            complement(1285..>1707)
                     /locus_tag="VGA49_03425"
     CDS             complement(1285..>1707)
                     /locus_tag="VGA49_03425"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX9664849.1"
                     /translation="RIRNIWFFKKYPIARQFIKFCLVGVSNTVIDFGVYLGLTRLFLF
                     WRTHFLIANFVAFSLAASWSYFLNKYWTFRDKEKFTQAQYLKFFLVSLVGLGLTQIVL
                     YSLVVAGLYDLFAKLIAVIVVVFWNFLANKYWTFRSRV"
BASE COUNT          562 a          364 c          281 g          500 t
ORIGIN      
        1 tcattataag ccggaatagt cgccacaact ttcataaaga aatcattaaa tagtttttct
       61 gactttttca gctttggcgt attcaattgt tttctccaga cctttttcca gggaaaaaac
      121 cggcagccag gctaattgat ccctggctct ggaaatatcc gggataccca gaggcgtcat
      181 aaaaaccaat ggctctttga aaacaacatt ggaagaagat ccagtcatat caataatctt
      241 gcgggcgaca tctgacagct tgaggtcaac gtcgctgccc aaattaaccg ggccaatatc
      301 ggccggcgcc gccatcaatc tgactaaacc gtcaaccaaa tcggtcacat aacttaaagt
      361 ggttctaaaa ttatcatcgc cgtaaatcac caaatcttta ttatccagag cgttaacaat
      421 aaaatcaggc atcatttccc cgtcattaag tctgttgcgc ggaccatagg ttctgaaaat
      481 tctggctatt ttggcgtcta aattatgaat atcttcataa gtattaacaa tggcttcagc
      541 gtattgtttg cctgaatcat aacaagcccg gggactcatt tgatttaaaa ttcccagata
      601 atcttcttta aaaattgtct gctgatctgt ccggccgccg taaaccactg aacttgagcc
      661 atgcaaaaat ttagctttat atttgatagc taaatcaagc atattcatca ggccgactgt
      721 attggcttta atggtatcaa ttttatattg atcaaaattt ttaggcgacg ttgggcaggc
      781 caggttataa acttcctgaa ttccctgaaa ctcaattcta aatctggcca gttccgggaa
      841 tttttccagc tccagcggct ggttaatgtc atgttttaaa aaaatgaaat caggaaatct
      901 caataaatga tcaatattgc cctgactgcc ggaaacaaaa ttatcaacac agataacttt
      961 gctggatttt aaaagatctt cgcacaaatg agagccaata aaaccggcgc cgccggtcac
     1021 taaaatattc tttttttcaa aaataggttc gggcataggt ttttgggtaa tggttaatgg
     1081 ttattagtga ttggttactg gttactaatg gctaataatt aagggattat aacaaaaaat
     1141 ggcaattttg acaaagtaat tttaattaat ttaaaaggcc agtttccggc cttttaactt
     1201 ttgatcatcc ccatctaata aaataattcc aacactatcc cctatcccct attaatctat
     1261 cccctatacc ctatacccta taccctatac cctgctccta aacgtccaat acttattcgc
     1321 caaaaaattc cagaaaacaa cgactataac ggcaatcaat ttggcaaaaa gatcatatag
     1381 cccggcaacc actaaactat acaaaactat ttgagttaaa cctagtccga ctaaacttac
     1441 caaaaaaaat tttaaatatt gggcctgggt aaatttttct ttatctctga atgtccagta
     1501 tttgtttaaa aaatagctcc aggaagcggc caggctgaaa gcaacaaagt ttgcgattaa
     1561 aaaatgagtt ctccaaaaca aaaacaaacg agtcagcccc agataaactc caaaatcaat
     1621 aacggtgttg ctgacgccga ccagacaaaa cttaataaat tgcctggcta ttggatattt
     1681 tttaaaaaac cagatatttc tgattct
//
LOCUS       DASWHZ010000173         4666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_316564, whole genome shotgun sequence.
ACCESSION   DASWHZ010000173 DASWHZ010000000
VERSION     DASWHZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316564"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGA49_03430"
     CDS             <1..291
                     /locus_tag="VGA49_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9664850.1"
                     /translation="SGMEIRVHQKIKDVNAKGEEKERVQVFEGLVIGRKGGQNQNATI
                     TVRKVSGGIAVEKIFPVYSPLITKFELLKTYKTRRAKLYFLRDHKKKLKEKK"
     gene            351..433
                     /locus_tag="VGA49_03435"
     tRNA            351..433
                     /locus_tag="VGA49_03435"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:385..387,aa:Leu,seq:gag)
     gene            580..783
                     /locus_tag="VGA49_03440"
                     /pseudo
     CDS             580..783
                     /locus_tag="VGA49_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            802..1167
                     /locus_tag="VGA49_03445"
     CDS             802..1167
                     /locus_tag="VGA49_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005891882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX9664851.1"
                     /translation="MIEEFTPLKTYLGKTIAVKIDRPLGSKHPEHDFIYPLNYGFISE
                     TISSNAAPIDVYVLGVFKPVKMINGKVIAVIKRKDDNTDKIVIAANNKNYTNEQIQAL
                     VEFQERFFNTKIISNHKGL"
     gene            1273..1512
                     /locus_tag="VGA49_03450"
     CDS             1273..1512
                     /locus_tag="VGA49_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9664852.1"
                     /translation="MFYCYIIQSVEEGRYYIGSSDSLKRVFEHNAGQVKSTKPHRPWK
                     LVYYETFQTRSQAFKRGKQIKAWKKRESIERLFKN"
     gene            1527..1599
                     /locus_tag="VGA49_03455"
     tRNA            1527..1599
                     /locus_tag="VGA49_03455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1560..1562,aa:Ile,seq:tat)
     gene            1610..1683
                     /locus_tag="VGA49_03460"
     tRNA            1610..1683
                     /locus_tag="VGA49_03460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1644..1646,aa:Val,seq:tac)
     gene            1815..2069
                     /locus_tag="VGA49_03465"
     CDS             1815..2069
                     /locus_tag="VGA49_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664853.1"
                     /translation="MRIILGIILAVVGAVITIKSEALFRTFGRVPFAEKYLGTEGGSR
                     LFYQLLGVLIIIIGFMFITNLVGGIFDWIGRSLFGGFAGP"
     gene            2162..2238
                     /locus_tag="VGA49_03470"
     tRNA            2162..2238
                     /locus_tag="VGA49_03470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2196..2198,aa:Ala,seq:tgc)
     gene            2590..3936
                     /locus_tag="VGA49_03475"
     CDS             2590..3936
                     /locus_tag="VGA49_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX9664854.1"
                     /translation="MQKITNFGGNVSFTSKNLYTPATDQEVLDILNKHQSGQIKVLSS
                     LHSWSEVTATSDVLIDLKKFKKIDLIEDQQGTTAEIGAGATLQEILNHLRQTSGYTLP
                     TLGGIKKQTIAGAIATGTHGTGRPSLSHYIEEVRLAAYDPATGQAKIYKFNSGQELLA
                     ARCSLGCLGVILSVKIRLAEKYWMSEQAKKYDTLNEVLAKKDDFPLQQFALFPYSWKF
                     YVYHRKITDKPKSLLKIKLYKIYDYLVVEIFLHLLVKLFQQLGRGAIYWWWQKAVPRL
                     LGQHQISGDSETILTLHTEHHYTFRHEEMEIFVPESNIFQAAEIIKKITTDYANSGQY
                     VHHYPIFFRYVLPEETLISMASGNQPYYSVSFFTYRPPQKRQPYYDYTLEAAKTLVQK
                     CQARIHWGKRNPLSYNDLKHLYPNLEEFKQLCQSVDPKGVFQNDYIRRVLGLELNY"
     gene            3939..4289
                     /locus_tag="VGA49_03480"
     CDS             3939..4289
                     /locus_tag="VGA49_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664855.1"
                     /translation="MSKSAFSVKVYSSYLFLMGAALVLVPNQLLQLLRFQATTEIWIR
                     VLGILTITVGFYYFYTARHEQTAFYRATVHGRLFFFGAICLLTFVFSQPLTLLAIGSI
                     DLLGAVWTWSALKK"
     gene            4373..>4666
                     /locus_tag="VGA49_03485"
     CDS             4373..>4666
                     /locus_tag="VGA49_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664856.1"
                     /translation="MDRQSIIEFLNSHCQIIDNPSVDKIRQLAKKDEVTTEFGGASYI
                     STTKNRSAKMTEAVFELTGQHQKLIDEVLEYLKNKELICLDRTLAPNSKINFLC"
BASE COUNT         1401 a          997 c         1013 g         1255 t
ORIGIN      
        1 tccggcatgg aaatcagagt ccatcaaaag attaaagatg tcaacgccaa aggcgaggaa
       61 aaagaacgag tccaggtctt tgaaggtttg gtgattggcc gcaaaggcgg tcagaaccaa
      121 aacgcgacca tcaccgtcag aaaagtttcc ggcggcattg ccgttgaaaa aatatttcct
      181 gtctattctc cactgatcac aaaatttgaa cttctcaaaa cttacaaaac ccggcgggca
      241 aaattatact tccttcgtga tcataaaaag aaacttaagg aaaaaaaata aattatttaa
      301 ataataggcg gatttatccg ccggagctcc gggcgaagca aggagcgtag gcggacgtgg
      361 cggaactggc agacgcgcag gcttgagggg cctgtacctt tttggtgtga gggttcgagt
      421 ccctccgtcc gcacttcgga ttttctcagg cagagccttc gtaaattctc agtgtcaaca
      481 ctttgactag tgtgtattta attataatga gaaacaaatg cgaagtgtcc tgagaaattt
      541 tgaaagcttt gcttgaaaaa ttacgaagga caatcaatta tgcattatct gtatatctta
      601 ttatgttcgg ataaaagtta ttatacggga atatcaaact ctatcccccg aagattaaaa
      661 gatcatagaa aaggcaaagt aatattcact agtaaaagat tgcctgttag gctagattac
      721 tttgaaaagt tcaatacaaa acaagaggct gctaaaagag aaagagagat taaaggatgg
      781 agtagaaaaa agaaaaaaac attgattgaa gaattcactc cattgaaaac atatttgggg
      841 aagactatag ctgttaaaat agacaggcct ttgggcagta aacatccaga acatgatttt
      901 atttatcctt taaattacgg ttttatttct gaaacaatat catcaaatgc agcaccaatt
      961 gatgtctatg ttttaggagt ttttaaacca gttaaaatga taaatggaaa agtaattgct
     1021 gttattaaaa gaaaagatga taatactgat aaaatagtaa tagctgccaa taacaagaat
     1081 tataccaatg aacaaatcca ggctttagta gagttccaag aaagattttt caatactaaa
     1141 atcatatcaa atcataaggg cctgtagctc agtggttaga gtcccgatcc gcctttggcg
     1201 gatcgtggat ccacgatttt tctgtcccga cctttggtcg gagacgaaaa atcgggacgc
     1261 cactctactg ttatgttcta ctgctatatt atacaatcag ttgaagaggg gcgttattat
     1321 atcggtagtt cagatagctt gaaaagagtt ttcgagcaca atgcggggca ggtaaaatcc
     1381 actaagcctc atcgtccatg gaaactcgtc tattacgaaa cctttcaaac aagaagtcag
     1441 gcctttaaaa gaggaaaaca aatcaaagcc tggaaaaaac gggaatctat tgaaaggtta
     1501 tttaaaaatt aaatagcacg gatcatgggc ctgtagctca gtggttagag cgccacgctt
     1561 ataacgtgga tgtcgatggt tcaattccat ccaggcccag attggttaag ggcaattagc
     1621 tcagctggtt agagcacttc gtttacaccg aaggggtcgt aggttcgagt cctacattgc
     1681 ccattcaagc agcaaaaggt tcctccgatt ttgtctccgc aaagcggagc cacaaaatcg
     1741 tgagtcctcg atcccgccaa aggcgggatc gggagaatcc tagtgcgccc atttaaaaaa
     1801 taaacaaaaa atttatgaga attattttag ggatcatttt agcggtcgtt ggcgcggtta
     1861 ttacaattaa atcagaagcc cttttcagaa catttggccg ggtgcctttc gctgaaaaat
     1921 atctgggaac tgaaggcggc agccggttgt tttatcagct cctgggcgtt ctgatcatca
     1981 ttattggttt tatgttcatc accaatctgg tcggcggcat ttttgactgg atcggccggt
     2041 ctttgttcgg tggtttcgcg ggcccgtagc ttagctggta gagcgcctgc tttgtcccga
     2101 tccgtttaat tttaccgaag gtaaaatttt aacggatcga ggatcctcga aaatttgtga
     2161 tgggcctgta gctcagttgg ttagagcacc tgctttgcaa gcagggggtc aagggttcga
     2221 atcccttcag gtccaccaca aattttcggg acaagcagga ggtcaccggt tcgaatccgg
     2281 tcgggtccaa acgtactgtt gaagataaaa aaatatcgcc tgaaggccca ccttaattta
     2341 tcaaggcaag tcccgatccg ccaaaggcgg atcgaggatc cacgatcccg ccaaaggcgg
     2401 ggtcgggaca gggggtcaag ggttcgaatc ccttcaggtt cgggacaagt cccgatcccc
     2461 cttagctgga tcgggacaga aggtcaacgg tttcccgcct atggcgggac cccgccaaga
     2521 ccgctttgcg gtcgtccggc ggggcgaatc tcctcgggtc caatttaaaa aagtatgtct
     2581 cccagaaata tgcaaaaaat cactaatttt ggcggcaatg tcagttttac ttccaaaaat
     2641 ctttatacgc cagccactga ccaggaagtt ctggatattt taaataaaca tcaaagcggc
     2701 caaatcaaag ttttatcatc acttcattcc tggagcgaag tcacagccac cagcgatgtt
     2761 ttgattgatt taaaaaaatt taaaaaaata gaccttattg aggaccagca gggaacaaca
     2821 gcggaaatcg gggctggcgc cactctccag gagatattaa atcatttacg tcaaacatcc
     2881 ggctatactt tgccgaccct gggcggcatc aaaaaacaaa ctattgccgg cgccatcgcc
     2941 accggcactc acggcaccgg ccggcccagc ctgtcccatt atattgaaga agtcaggttg
     3001 gccgcctatg atccggccac cggtcaggct aaaatttata aattcaacag cggtcaggag
     3061 cttttagcgg cccgctgttc gctgggttgt ctgggagtca ttctgtcagt aaaaatccgg
     3121 ctggctgaaa aatactggat gagtgaacag gccaaaaaat atgatacctt aaatgaggtt
     3181 ctcgcaaaaa aagacgattt tcctcttcag caattcgctc tgtttcctta ctcctggaaa
     3241 ttttatgttt atcaccgaaa aattactgat aaaccaaaaa gtttactgaa aattaaactg
     3301 tataaaattt acgactattt ggtggtggag atatttttgc acctgctggt taaactgttt
     3361 caacaattgg gccggggagc catctactgg tggtggcaaa aagctgtccc ccgcctgctg
     3421 ggtcagcatc agatcagcgg cgacagcgaa accatcctga ctttgcatac tgaacaccac
     3481 tatactttcc ggcatgaaga aatggaaatt tttgtgccgg aatcaaatat ttttcaggcg
     3541 gccgaaatta tcaaaaaaat tacgactgat tacgccaaca gcggtcaata tgtccaccat
     3601 tatccgattt tttttcgcta tgttctgcca gaagagacgc taatctcaat ggcttccggc
     3661 aatcagcctt attattcagt cagttttttt acttaccggc cgcctcaaaa acgccagcca
     3721 tattatgact acaccctgga ggcggctaaa acattggttc aaaaatgcca ggccaggatt
     3781 cactggggca agcgaaatcc tttgagctat aatgatttaa aacatctcta tcctaacctg
     3841 gaagagttta aacagctctg ccagtcagtt gatcccaaag gtgtttttca aaatgactac
     3901 atcagacggg ttctgggctt agaattaaat tactaactat gtccaaatca gctttcagcg
     3961 tcaaagtcta cagctcctat ctttttctaa tgggcgcggc tttagtcctg gttccaaacc
     4021 agcttcttca actgctgcgt tttcaggcaa ccactgaaat ttggatcaga gttttgggaa
     4081 tattgaccat tactgtcggc ttttactatt tctacactgc ccgccatgag caaaccgcat
     4141 tttaccgggc gaccgtccac ggccggctgt tcttttttgg agccatctgc ctcctaacct
     4201 ttgtcttcag ccaaccgttg actttgctgg ctatcggcag cattgatctt cttggcgctg
     4261 tttggacctg gtcagctcta aagaaatagt ttaaacaata aaaaaagctt ttcaaaaaag
     4321 tcttttttgt tgccgtcatt tatgttatac tgctaaaatt aaatttaaaa ctatggaccg
     4381 tcaatcaatt attgaatttc taaattccca ctgccaaatt attgataatc cgtcagttga
     4441 taaaatcaga cagctggcta aaaaagatga agtcaccact gaatttggcg gcgcctctta
     4501 tatcagcaca acaaaaaacc gcagcgccaa aatgactgaa gcggtatttg agctgacggg
     4561 ccagcatcaa aaactgattg atgaggtttt agaatattta aaaaataaag aacttatttg
     4621 tctggaccgg acactggccc ctaattcaaa aattaatttt ctttgt
//
LOCUS       DASWHZ010000174         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3290328, whole genome shotgun sequence.
ACCESSION   DASWHZ010000174 DASWHZ010000000
VERSION     DASWHZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3290328"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VGA49_03490"
     CDS             complement(<1..341)
                     /locus_tag="VGA49_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664857.1"
                     /translation="MNYFSEKMYLMVLFALVIVFTVLFAGTYIYKHFYFYKDNLIKYF
                     PRTTAAYAHLTLNEQVKKDFLENQGLLSQLEQFRLAEIFKNINREVSLGFIPVNEQLA
                     AVVVFDVDNFN"
     gene            complement(523..1680)
                     /locus_tag="VGA49_03495"
     CDS             complement(523..1680)
                     /locus_tag="VGA49_03495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9664858.1"
                     /translation="MKIYLIGQKGIPAQAGGIERHVEELSTRLAQSGHEVFVYCRPYY
                     ASTADKNYQGVNLIKLPTIKTKHLDAIVHTFLATLDVLRKEADVIHYHGIGPASLSFI
                     PKLFKRKARLIFTFHCQDYKHQKWGAFARAYLKLGELMGCRTADKIITVSQTLKDYVL
                     QKYHLEAVYIPNGLKLPQLGNAKNDLLKKWGLTPDNYILTVNRLVRHKGIHHLIKAYQ
                     QTKTDKKLVIVGGSAYTDNYVNQLKELAGQNDRIVFTGERVNGDLAELFSNAYLYVHP
                     SEAEGLSIAILEALGYGKTVLVSDIPENQEIVKNVGFLFKNKDTENLAAQLRFLLDNP
                     YIVQATSGLGREHVSKNFNWQTIARETMKVYQADLSRRRLIGREVYGQLTK"
     gene            1911..2129
                     /locus_tag="VGA49_03500"
     CDS             1911..2129
                     /locus_tag="VGA49_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664859.1"
                     /translation="MNVTQQQFNRLATKEDVETLNDKIRDIQKQLLSDWRFSSVRKDL
                     KEIKLDNQISTLEHKLNRLIEMLDKKTN"
     gene            2141..2548
                     /locus_tag="VGA49_03505"
     CDS             2141..2548
                     /locus_tag="VGA49_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664860.1"
                     /translation="MSITKKDLQKFATKHDLKKLEKKIDGQIDFLARQINTNTARLDN
                     MYTKEKLDKKFEKIDNQFKMMADQMDKRFEKVDNQFKMMADQMDKRFEKVTSGNDKII
                     KMFEKRELEDASQKFITDNLEARVFDLETKILK"
     gene            2578..>3132
                     /locus_tag="VGA49_03510"
     CDS             2578..>3132
                     /locus_tag="VGA49_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9664861.1"
                     /translation="MPASMFFLIFSASPPRFAERGRGGGSTACRHGAQPLAEIFNLKH
                     MKKILISALLFLPIITASAQTDHIIITEIQIAGQTSQDEFVEIYNPTNEEINLADWRL
                     TKKTASGNEYNLLTKFPEIILPPNQHLLITHPTAYTGLVEADLTYSTSQSIAADNTII
                     LYSDAGQTVADQVTLIVKPDNLESL"
BASE COUNT          975 a          689 c          614 g          854 t
ORIGIN      
        1 tcattaaaat tgtcaacgtc aaaaacaacc accgccgcta actgctcatt gaccgggata
       61 aatcccagag aaacttcccg gttaatattt ttaaaaattt cagccagcct aaactgctcc
      121 agctgactta acagcccctg attctctaaa aaatcttttt tgacttgttc atttaaagtc
      181 aggtgagcgt aagcggccgt tgtccggggg aaatatttaa ttaaattatc tttataaaaa
      241 taaaaatgct tataaatata agtgccggca aacaaaaccg tgaaaacaat caccagggcg
      301 aataaaacca tcaggtacat tttttcgctg aaataattca tagtggttac attttacacc
      361 aatgaataac cgcgtcgcaa gcagcgcgat atctattact gcttgcggca ggcggttatt
      421 cattatctgc acgccgcttc atccccgcgg caagccgcgg ggtattcgcg ggcagataat
      481 aaaaaaaccc cggcaacgca gccggggctt tcaaacttaa atttattttg taagctggcc
      541 gtaaacttcc cggccaatca gtcgccggcg ggataaatca gcctggtaaa ccttcatggt
      601 ttcccgggca attgtctgcc agttgaaatt tttactgaca tgttcccggc ccagaccgct
      661 ggtcgcctgg acaatatacg gattgtccag caaaaaccgc agctgggcgg ctaagttttc
      721 agtgtcttta ttcttaaaca gaaagccgac atttttaaca atttcctgat tttcaggaat
      781 atcgctgacc agaactgttt tgccgtaacc caaagcttcc aaaatcgcaa ttgacaaacc
      841 ctcggcttca gacgggtgga catataagta agcgttgctg aacagctccg ccaaatcgcc
      901 attaacccgc tcgccggtga aaacaatccg gtcattctgc ccggccagtt ctttcagctg
      961 attgacataa ttgtcagtgt aagctgatcc gccgacaatc accagcttct tgtcagtttt
     1021 agtttgctgg taagctttaa ttaaatgatg aatccctttg tgtctgacca gccggttaac
     1081 tgtcagaata tagttgtccg gcgtcaagcc ccactttttt aataaatcat tctttgcgtt
     1141 gcctagctgg ggaagtttca aaccgttggg aatataaacc gcttccagat gatatttttg
     1201 taaaacgtaa tcttttaacg tttgagaaac ggtaataatc ttgtcagccg tccggcagcc
     1261 catcagctcg cccagcttta aataagctct ggcgaacgcg ccccattttt gatgtttata
     1321 atcctggcaa tgaaaggtaa aaatcagcct ggcttttctt ttgaaaagtt tggggataaa
     1381 agacagtgac gccgggccaa tgccgtgata atgaatgaca tcggcttctt ttcttaaaac
     1441 atccagggta gccagaaagg tatggacgat ggcgtccaga tgtttggttt taatcgttgg
     1501 cagtttaatc aaattgactc cctggtaatt tttgtcagcg gttgaagcgt agtaaggccg
     1561 gcaatagaca aaaacctcgt ggccgctttg ggctaaacga gttgacagct cttcaacatg
     1621 gcgttcaatg ccgccagcct gggctggaat ccctttttgt ccgataagat aaattttcat
     1681 agataccgaa cattaacaca tttttttcag tttgtcaagg gtgattactg ccggcttccg
     1741 ccctctccta atgtcaggct tgtctttttg gctctttaaa aattattaaa atgaagagcc
     1801 gtctctttct ccgccgtcgc taaaatctgt agataactac cggttgacca gactgttcaa
     1861 tttgttatca tgtaattaca tttatcaggc agctgcctgt caaaaagctt atgaatgtca
     1921 ctcaacaaca atttaataga ttagcgacta aagaagacgt ggaaacttta aacgataaaa
     1981 ttcgcgacat ccaaaaacag ttgttaagtg actggagatt ttcatctgtg cgtaaagacc
     2041 taaaagaaat taaacttgat aatcaaattt caactttgga acataaattg aaccggctca
     2101 ttgaaatgct ggacaagaag accaattaag gaataaatat atgtcaatca ctaaaaaaga
     2161 cctgcaaaaa tttgccacca agcatgatct aaaaaaatta gaaaagaaaa ttgatggaca
     2221 aatcgatttt ttggctcggc agataaacac aaatactgcc agacttgaca atatgtatac
     2281 aaaagaaaaa ctggacaaaa aatttgaaaa gatagataat cagtttaaaa tgatggctga
     2341 tcaaatggac aagagatttg aaaaggtaga taatcagttt aaaatgatgg ctgatcaaat
     2401 ggataagaga tttgaaaagg ttaccagcgg caacgataaa attataaaaa tgtttgaaaa
     2461 aagagaatta gaagacgctt cccaaaaatt tattacagat aatctagaag ctcgggtctt
     2521 cgaccttgaa acaaaaattt taaaataact taacttaaaa ctaaacaaat cataaacatg
     2581 ccagcaagca tgtttttttt aattttttcc gccagcccgc cccgcttcgc ggagcgaggc
     2641 cggggaggcg gatccacggc atgccgccat ggcgcgcagc ctttggctga aatttttaac
     2701 ctgaaacata tgaagaaaat tttaatttct gctttattgt tcctgccgat aatcacagcc
     2761 tcggcccaaa ccgatcacat aattatcacc gagattcaaa tagccggcca gaccagccag
     2821 gacgagttcg tggaaattta taatccgacc aacgaggaaa tcaatctggc tgactggcgg
     2881 ctgaccaaaa aaaccgccag cggcaatgaa tataatttac taactaaatt ccctgaaatc
     2941 attttacctc caaatcagca tcttttaatc actcacccca cagcctatac tggccttgtt
     3001 gaggctgacc tgacttactc cacttcccag tcaattgccg ctgacaatac aatcattctt
     3061 tattcagacg ccggccagac tgttgctgat caggtgactt taattgttaa gcctgacaac
     3121 ctggaaagcc tg
//
LOCUS       DASWHZ010000175         2714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2206957, whole genome shotgun sequence.
ACCESSION   DASWHZ010000175 DASWHZ010000000
VERSION     DASWHZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206957"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..604
                     /gene="rpsL"
                     /locus_tag="VGA49_03515"
     CDS             188..604
                     /gene="rpsL"
                     /locus_tag="VGA49_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9664862.1"
                     /translation="MPTINQLIKKPRKQTRKKSKTPSLQYVLNSLKRKKNKLTKGSAF
                     KRGVCLKVTTVTPKKPNSALRKIARVRLSNGMEVTAYIPGIGHNLQEHSIVLIRGGRV
                     KDLPGVRYHIVRGVYDTQGVDGRKQSRSLYGAKKPK"
     gene            626..1093
                     /gene="rpsG"
                     /locus_tag="VGA49_03520"
     CDS             626..1093
                     /gene="rpsG"
                     /locus_tag="VGA49_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9664863.1"
                     /translation="MRGKKAPKRKIQPDIRYSNVNVEKFINYLMVGGKKSVARGVVYG
                     AFDIIKDKTKINPVELFDKAFKNISPVMEVRGRRIGGANYQIPVQVRTERRFLLSARW
                     LIEAAQARKGRSMAQKLAEELMDAANQTGAAIKKKENVHKMAESNRAFAHFAR"
     gene            1107..>2714
                     /gene="fusA"
                     /locus_tag="VGA49_03525"
     CDS             1107..>2714
                     /gene="fusA"
                     /locus_tag="VGA49_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9664864.1"
                     /translation="MAREYPLEHVRNIGIIAHIDAGKTTVTERILFYTGKKHKIGEVH
                     EGEAEMDWMEQERERGITITSAATTCFWKDYRINIIDTPGHVDFTAEVERSLRVLDGG
                     VVVFDGVAGVEPQSETVWHQADKYMVPRICFINKMDRTGADFYKDLNSIHERLSKKAV
                     AVQLPIGSAETFQGIIDLLEQKARIFLDDLGVKWEDKEIPDDLKEKAAEFRAKLIEAV
                     VEHDEDTMNKYLDGQEISLEELKKVLRKATLKYELVPVFCGSALKNKGVQFLLDGIVE
                     YLPSPQDVPPIKGVHPDNHETVIERKVDDSEPFTGLAFKIATDPYVGKLCFFRVYSGK
                     ISSGSYILNATTGKKERIGRIVRMHANSREDVEEVYSGEIAAAVGLKETFTGHTLVDE
                     NHPVLLETITFPEPVISVAIEPRTKADQEKMGIALSKLSEEDPTFRVKTDDETLQTII
                     SGMGELHLEIIVDRMKREFKVEANIGKPQVAYKETIQKTAEAEGKYVRQSGGRGQYGH
                     CWLKVEPQERGADFEFVNAIKGGTIPQE"
BASE COUNT          809 a          568 c          658 g          679 t
ORIGIN      
        1 atcaaaggct cataagcctg ccgccaacca cccatggaaa caaagaggtt gcctcaagac
       61 aacagtgaag caaaaagaac ctggtatgac attttaaaat acttgaaact atgacatttt
      121 aacttcctgt tgacaaatag atttaatatt cttgactgaa attacccttt atgttaattt
      181 acaatctatg cctaccatta atcaacttat caagaaaccg cgcaagcaaa ccaggaaaaa
      241 gtcaaaaact ccgtctttgc agtatgtttt gaatagttta aaaagaaaga aaaataaact
      301 gaccaaaggg agcgctttta agcgcggcgt ttgtttgaaa gtgacgacag ttactcctaa
      361 aaaaccgaac tccgctctcc gaaaaattgc cagagtcaga ttgtccaatg gcatggaagt
      421 gaccgcctat atccctggta ttggtcataa tctgcaggaa cattcaattg ttttaattcg
      481 cggcggccgg gtcaaagatc tgcctggcgt ccggtatcat attgtccggg gcgtttatga
      541 cacccagggc gttgacggtc gaaaacaaag ccggtcgctt tacggagcca aaaaaccaaa
      601 ataagacact agccactaaa attctatgcg cggaaaaaaa gctccaaaac gaaaaattca
      661 acctgacatc cgctacagta atgttaatgt tgaaaaattc attaactatc ttatggttgg
      721 cggcaaaaaa tcagtcgccc gcggcgtggt ttacggagct tttgatatta ttaaagataa
      781 aaccaaaatt aatccggttg agctgtttga taaagcgttt aaaaatattt caccggtcat
      841 ggaagtcagg ggccgccgga ttggcggcgc caattatcag attccagtcc aggtcagaac
      901 agaacgccgg tttttattgt ctgcccgctg gctgattgaa gccgcccagg ccaggaaagg
      961 ccggtcaatg gcccagaagc tggctgaaga attaatggac gccgctaacc agaccggcgc
     1021 cgctattaag aaaaaagaaa atgtccacaa aatggctgag tcaaaccggg cttttgctca
     1081 ttttgcgcgc taaggagttt tagtttatgg cccgggaata tcctttagaa cacgtcagaa
     1141 atattggaat catcgctcat attgacgctg gcaaaacaac cgttaccgaa cggattcttt
     1201 tttatacggg caaaaaacat aaaattggcg aagttcacga aggcgaggct gaaatggact
     1261 ggatggaaca ggaacgggag cgggggatta ccattacatc ggccgccacc acctgttttt
     1321 ggaaagacta ccggatcaac attattgaca cgcctggcca cgttgacttt acggctgaag
     1381 ttgagcggtc tttaagagtt ttggacgggg gcgtggttgt ttttgacggc gtcgccggcg
     1441 ttgagcctca gtcagaaaca gtctggcatc aggctgataa atatatggtg ccccggatct
     1501 gttttattaa taaaatggac cggaccggcg ctgattttta taaagattta aactcaatcc
     1561 acgagcggct gtccaaaaaa gccgtggctg tccagctgcc aattggttca gctgagacct
     1621 ttcagggcat tattgatttg ctggagcaaa aagcccggat ttttctggat gacctgggtg
     1681 ttaagtggga ggataaagaa attcctgatg atctaaaaga aaaggcagct gaatttcggg
     1741 ccaaactgat tgaagctgtt gttgagcacg atgaagatac aatgaataaa tatctggacg
     1801 gtcaggaaat ttctctggaa gaactgaaaa aagttttaag aaaagccact ttaaaatatg
     1861 aactggtgcc ggttttttgc ggttcagccc tgaaaaacaa aggggttcag tttttgctgg
     1921 acggtatcgt ggaatatttg ccgtcacccc aggacgtgcc gccgattaag ggtgttcacc
     1981 cggacaatca tgaaactgtc attgaaagaa aagttgatga ctctgaaccg ttcaccggac
     2041 tggcttttaa aatcgcgact gatccttatg ttggcaaact atgttttttt cgggtttatt
     2101 cgggcaagat ttcatccggg tcgtatattt tgaacgccac taccggcaaa aaagaaagaa
     2161 tcggccggat tgtcagaatg cacgccaatt cccgggaaga cgttgaggag gtttactccg
     2221 gagagattgc cgcggcggtt ggtttaaaag aaacttttac cggccatact ttggtggatg
     2281 aaaatcatcc ggttctgctg gaaactatca ctttccccga gccggtgatt tcggtcgcta
     2341 ttgaacccag gaccaaagct gatcaggaaa aaatgggtat tgccttgtcc aagctgtctg
     2401 aagaagatcc aactttccgg gttaaaaccg atgatgaaac tttgcagaca attatttccg
     2461 gcatgggcga gcttcatctg gaaattattg ttgaccggat gaagcgggaa tttaaagttg
     2521 aggccaatat tggcaaaccc caggttgcct ataaagaaac tattcaaaaa accgctgaag
     2581 ctgaaggcaa atatgtccgg cagtcaggcg gccgcggtca gtacggccat tgctggctga
     2641 aagtggaacc gcaggagcgc ggcgctgatt ttgaatttgt taatgccatt aaaggcggaa
     2701 ccattcccca ggaa
//
LOCUS       DASWHZ010000176         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1442443, whole genome shotgun sequence.
ACCESSION   DASWHZ010000176 DASWHZ010000000
VERSION     DASWHZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442443"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /locus_tag="VGA49_03530"
     CDS             complement(<1..2549)
                     /locus_tag="VGA49_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664865.1"
                     /translation="MVKYSRTYNKNQTSFKICRQLHCYLLTKSKFYYSWHKDNHESLH
                     WSVLVLYLFGFLIVMTAFVYPGYIYPQAPSILEQQTLLLRTTDQGELTLIEHNPQSVP
                     EDFYLSLNPGEFAEPASIKIINGQIYVPYKVSFVGRSYEVEWKSKNGTQKLTIPTNDL
                     KISLFEKINGGYQLKASSQPVYQYARETIREEIPVSQEVFPDLAPIQEEESELQPESE
                     ITQEEINVKDQSTPGGEVLTEEAFPSEQEEAASEETLIEKIPLAEPSPGEELTEDEEG
                     LANQPPPVEVPPEKDLFEEGLSEEDEESLTKVLPESSSPEEEKTGETLPEAESPEEDF
                     SPESEIPAEINGQLEELIEEVQEAVDEIMAETAEEPAIEEVVPEVSQEAEAAPPADSG
                     DVVAASIFRIAFETVKRGVQFVFATIFPDFDEIFSLEERRILEQLELEKEQPSIEWLD
                     YEIDQNSIRVFNESFANYNLTILYYQLTDQNNNRTDAEISIKIGFDGQKYINQWTYQI
                     QPNNYVNRMNWEVSLADDSLIREDNFDVTDRDLLSAPSLMSVDSDEDGLTDDQESFLG
                     TDPLKKDTDQDGFNDRLEVDSRHNPLNNFILEDLYFESIRGDNIFRSQGETEGSMGDE
                     GEAGGSMRDMEETEGIKGNQGEAEGETEEVPIEEETALTIIEEVEIVPEEAITEETGP
                     AIIEEEETVPEEAITEETAPTIIEEGETLEAVKIEETITAEETAVVEAEKPKLTPAEI
                     QTAVLSQLDSGEGLKKLRFDDLALNWEENERSTRVSQSATDNNNQVLFHQTSLRDSFA
                     VKSALGSLQPDQLVTVNSKDRFEISFNLNQGGIITSWYDLENDPNRENQLA"
     gene            complement(2669..3931)
                     /gene="murF"
                     /locus_tag="VGA49_03535"
     CDS             complement(2669..3931)
                     /gene="murF"
                     /locus_tag="VGA49_03535"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX9664866.1"
                     /translation="MFKKITKLVIKKYKPDIIGITGSVGKTSTKEAVFAVLKTKYRVQ
                     QNDNYDNLDLTGVFINYDNKSVKILKNFWLFLRGLKLLFKKDKNFPQVFVLDLPATQT
                     GDIKNIVQLLSFKIGLITAIDRSHLENFTSLDKMIREISYLVSGLGPDRFLILNRDDQ
                     DVFDLMTKSKAAVKDYGFADESGIRASEVVTSILKINNQEKEEVEKIEGQSFKLTYQG
                     STVPVHLPRVLGRQHIYAALAGAAVGLIYNLNILEISQGLNSYQPPRGRINLIPGVKN
                     TLIIDDTFNASPLSAKASLEVLANFELPAGRQKFVVLGDMAGLGGKTEEAHYQLGQRV
                     ARLEAASLVAVGEKSRDIIRGAKDQGMPDDFCFHFPDTEQAGKFIQNKMKKGDLIFID
                     GSAAMRMEKIVKELMAEPLRAPELLVRR"
     gene            complement(3989..>4216)
                     /locus_tag="VGA49_03540"
     CDS             complement(3989..>4216)
                     /locus_tag="VGA49_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9664867.1"
                     /translation="GKTGTAQVSSTVRAGYSGKTIHSFVGFAPVDDPAFVMLVKLDDP
                     KDVPFAASSAAPLFGEIARFLLNYYQIPPDY"
BASE COUNT         1133 a          991 c          788 g         1304 t
ORIGIN      
        1 tcagccagct gattttcccg gttgggatca ttttccagat cgtaccagct ggtgataatc
       61 ccgccctggt ttagattaaa actaatttca aatctgtctt tggaattaac agtaaccagc
      121 tgatcgggct ggagactgcc cagagccgac ttaaccgcga aagaatcccg gaggctggtt
      181 tgatgaaaca aaacctgatt attgttgtca gtggcggatt gagaaaccct ggttgatctt
      241 tcattttctt cccagttcaa agccagatca tcaaaacgca gttttttaag accctctccc
      301 gaatcaagct ggcttaaaac ggctgtttgg atttcagccg gagttaattt cggtttttca
      361 gcttcaacca cggcagtttc ttcggctgtt atagtttctt ctattttaac agcttctaat
      421 gtctccccct cttcaattat tgtcggagct gtttcttccg tgattgcttc ttcaggaaca
      481 gtctcctcct cttcaatgat tgcaggacct gtttcttccg tgattgcttc ttcaggaaca
      541 atctcaacct cttcaatgat tgtcagagct gtttcctctt caattggtac ttcctctgtc
      601 tccccctctg cttccccttg attccctttg attccttccg tttcctccat atccctcatg
      661 cttccccctg cttccccttc atcccccatg cttccctctg tctccccctg cgacctaaaa
      721 atattgtctc ctctgattga ttcaaaatac aaatcttcca aaataaaatt atttaaaggg
      781 ttgtgccggc tgtcaacctc caggcgatcg ttaaagccgt cctggtcggt atcttttttc
      841 aaaggatctg ttcccaaaaa actttcctga tcatcagtca agccgtcttc gtcgctgtca
      901 accgacatta agctgggcgc gcttagtaaa tccctgtcag tgacatcaaa attatcttcc
      961 ctgatcagac tgtcatcagc cagagaaact tcccaattca tccgattgac ataattgttg
     1021 ggctgaatct gataggtcca ttgattaata tatttttgcc cgtcaaaacc aattttaatt
     1081 gatatctcgg cgtcagtccg gttattgttt tgatcagtca gctgataata aagaatggtt
     1141 aaattgtagt tggcgaaact ttcgttaaaa acccggattg aattctgatc aatttcataa
     1201 tcaagccatt caattgacgg ctgttctttt tccagctcaa gctgttccaa aattcttctt
     1261 tcctccaggc tgaaaatctc atcaaaatca ggaaaaatag tcgcaaaaac aaattggact
     1321 cctcttttga ctgtttcaaa agcgattcta aaaatactgg cggccaccac gtccccgcta
     1381 tcagccggcg gggcggcttc agcttcctgg ctaacttcag gaacaacttc ttcaattgcc
     1441 ggttcttcag cggtttccgc cataatttca tcgacggcct cctgaacttc ttcaatcaat
     1501 tcttccagct gaccattaat ctctgccgga atttcagatt caggagaaaa atcttcttca
     1561 ggcgattctg cttcaggcaa cgtctcccct gtcttctctt cctctggtga agagctttcg
     1621 gggaggactt tggtcagcga ttcttcatct tcttcagata agccttcttc aaacaaatct
     1681 ttttctggcg ggacttcgac gggcggaggc tgattagcta agccctcctc atcttcagtc
     1741 aattcttctc caggcgacgg ttccgccaaa ggaattttct caattaaggt ttcttcagag
     1801 gcggcctcct cttgttcaga cggaaatgct tcctctgtca gaacttcccc gcccggcgtt
     1861 gactgatctt ttacgttaat ttcttcttga gtaatttctg attctggctg aagttctgat
     1921 tcttcttcct ggattggcgc caaatccgga aaaacttcct gactaaccgg tatttcttct
     1981 ctgattgttt cccgggcgta ctgataaacc ggctgactgg aagcttttaa ctgatacccg
     2041 ccgtttattt tttcaaataa actaattttg agatcattag ttgggattgt taatttttga
     2101 gtgccgtttt ttgatttcca ttcaacttca tagcttctgc cgacaaaact aactttataa
     2161 ggcacataaa tttgaccgtt aattatctta atgctggccg gctcggcaaa ctctccggga
     2221 tttaaagaca aataaaaatc ctccggaact gattgaggat tatgctcaat cagggttagt
     2281 tcgccctgat cagttgttct taaaagcaaa gtttgctgtt caagaattga cggagcttgc
     2341 ggataaatat agccgggata aacaaacgcc gtcatcacta tcaaaaaacc gaataaatat
     2401 aaaactaaga ctgaccagtg aagagactca tgattatctt tatgccagct gtaataaaat
     2461 ttagatttag ttagtaaata acaatgcagt tggcgacaaa ttttaaatga agtttggttt
     2521 ttgttgtaag tccggctgta tttaaccata tatcaaaaaa ttcgatgtga aatcaaaaca
     2581 tctttggtct aattttacaa taaagggaaa gttttaacaa accaaaaacc ctcccggagg
     2641 aggattcaat tgctgacaat ttaaaaaatt aacggcggac aagcagctcc ggcgcccgca
     2701 acggttcagc catcagttct ttgactattt tttccattct cattgccgcc gagccgtcaa
     2761 taaaaatcaa atctcctttt ttcattttat tctgaataaa tttaccggcc tgttcagtat
     2821 caggaaaatg aaaacaaaaa tcatcaggca tcccctgatc tttggctcct cttataatat
     2881 cccgcgactt ttctccaact gccaccaaag aagcggcttc cagccgggcg actctttgtc
     2941 caagctgata atgggcttct tcagttttac cgcccaaacc ggccatatcg cccaggacaa
     3001 cgaatttttg cctgccagct ggcagttcaa aattagccaa aacttccagt gacgctttgg
     3061 ctgataatgg tgaagcgtta aaagtatcgt caatgattaa tgtgttttta acgccaggta
     3121 ttaaattgat ccggccgcgg ggaggctggt agctgtttag tccctgggaa atttccagaa
     3181 tattcagatt ataaatcaaa ccaacggccg ctccggccag ggcggcgtaa atatgctgac
     3241 gacccaaaac ccggggcaaa tgaaccggca cggtactgcc ctggtaagtc agtttaaaac
     3301 tctggccttc aatcttttca acctcttctt tctcctgatt attaattttt aaaatgctgg
     3361 tgaccacttc actggctctg atccctgatt catcggcaaa accgtaatct ttaaccgccg
     3421 ccttgctttt tgtcattaaa tcaaagacat cctggtcatc ccggtttaaa ataagaaacc
     3481 gatccgggcc aagtcctgaa actaaatagc tgatttccct gatcatttta tccagactgg
     3541 tgaagttttc cagatgactc cggtcaatgg cggtaatcag gccaatttta aaactcaata
     3601 attgaactat atttttaata tctccggttt gagtcgctgg caaatcaagc acgaaaactt
     3661 gaggaaaatt tttatctttt ttaaacagca gtttcagccc tcttaaaaac agccaaaaat
     3721 ttttcaaaat ttttacagac ttattatcat aatttataaa aactccggtt aaatccaaat
     3781 tgtcataatt gtcgttctgt tggacgcggt attttgtttt taaaaccgcg aaaaccgctt
     3841 ctttggttga agttttgcca acactgccgg taatgccgat aatgtcaggt ttatattttt
     3901 taataactaa ttttgtaatc tttttaaaca taaagtaagg gggattgaat gatcttttaa
     3961 cttaactgat tagggacaaa aaagcaactt aatagtccgg ggggatttga taataattta
     4021 gtaaaaacct ggcaatttca ccaaataaag gagcggctga cgaagcggca aaagggacgt
     4081 ctttggggtc atccagcttg accagcatca caaaagccgg atcatcaacc ggagcaaacc
     4141 cgacaaaaga atgaatagtc ttgccggaat aaccggcccg gacagtactt gaaacctggg
     4201 cggtgcctgt tttccc
//
LOCUS       DASWHZ010000177         6873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_1838340, whole genome shotgun sequence.
ACCESSION   DASWHZ010000177 DASWHZ010000000
VERSION     DASWHZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838340"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VGA49_03545"
     CDS             complement(<1..350)
                     /locus_tag="VGA49_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9664868.1"
                     /translation="MSLKRLIQTIEENKLPVKIAEIKRAFKFADEAHQGQKRLSGEPY
                     FIHPSAVATILAEMKADQPTIIAGLLHDVVEDTDAALSELERKFGKEVANLVDGVTKL
                     RGVHYSGDENYVEN"
     gene            complement(497..2749)
                     /gene="topA"
                     /locus_tag="VGA49_03550"
     CDS             complement(497..2749)
                     /gene="topA"
                     /locus_tag="VGA49_03550"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX9664869.1"
                     /translation="MKNLVIVESPTKAKTISKFLGRDYKVLSSYGHVRDLPKKEMGVD
                     VEHNFEPTYVIPEKAKTRIKALKEAAKKSDVIILATDEDREGEAIAWHLIKAIGIDKK
                     KTQRIVFHEITKSAIEKALKEPRDINIDLVDAQQARRILDRLVGYELSPLLWKKVARG
                     LSAGRVQSVAVRLIVDREREIKAFKQEEYWTIESELKKSAAGGQEGAESFLAKLVKQS
                     DKNLDKFAVKNQAAASQILKDLEKADYQVDKIAKQEKIRKPNPPFKTSTLQQEAANRL
                     GFSTKQTMVLAQQLYEGISIGSEGSVGLITYMRTDSLNLSAEAVKNTRAVISQQFGDQ
                     YLPGKPVFYKTTAKGAQEAHEAIRPTFPNKTPESVKQYLDKNQFRLYDLIWRRTIACQ
                     MNPAIMDNTSVDIKAKNYIFRATGSVIKFDGFLKIYPAQAKEKLLPLLAEKEILDLIK
                     LIPSQHFTEPPARYNEAGLVKALEEEGIGRPSTYAPTISTIQDRNYVIKNEDKRLQPT
                     DIGEVVNDLLVAHFPKIVDLKFTAEMEESLDQIARGKKDWAPMIKNFYQPFKKTITEK
                     GKELKKEEITEEKTDQVCDKCGSPMVIKIGRYGKFLACSNYPECKNTKPLPGEGKNGQ
                     PPEPETTDEVCDKCGSPMVIKTGRFGKFLACSNYPDCKNTKPIVKSTGLKCPECGRGE
                     IVEKRSKKGRVFYSCSEYPDCKFALWQKPTGEKCPECGSLLVFGGKNKVKCSNKECAF
                     KKEIEETIKT"
     gene            complement(2763..4103)
                     /locus_tag="VGA49_03555"
     CDS             complement(2763..4103)
                     /locus_tag="VGA49_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9664870.1"
                     /translation="MFQNYFQTIKFLESLDNLPNQITDDYQKSRRLNLKRFRYFLKLI
                     GNPHRDFKYVHVGGTSGKGSTVSMIQSVLTAAGYKTGAYFSPHPTTTIERIRINNHYI
                     STKNFSAIIAGLSEPIKKVYLKTSYGRPSYFEILLAAAFLYFKKEKCDYVILEVGCGG
                     EFDPTNIIQKPKFTIITNVNHDHTHILGKVLKKIAETKAGIIKPNSIFLTTETRPAIL
                     NIFRSVCRQKKAKFNQIKSATKLIKNDFDKAEFEFENEIYQLAMGGRHQLKNALLAIN
                     LANKLGIKKSIIKKGLLAGRLPGRAEKIQSKPDVIIDIAHNDSKIKSLVNILKDLTEQ
                     RLFLIIGITESKDSSRMLKKIIPLADQVLLTRYQVSRRPAADLKKLARQVKKISKNKL
                     VPKFFLNPFAALDYALGQAGKNDLIVITGSTYLAGELRQRWIPEEKIVKSRKSF"
     gene            complement(4158..5261)
                     /gene="dprA"
                     /locus_tag="VGA49_03560"
     CDS             complement(4158..5261)
                     /gene="dprA"
                     /locus_tag="VGA49_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9664871.1"
                     /translation="MSNCLNSESKHYLGFSLIENLGPVNTRKILKYFKSIEAAWRGSF
                     DDFIRAGLGEKLAREIINQRQMIDPEQELVKIQKHKISLINIENKNYSALLKEIYAPP
                     PLLYYQGNIRILNQLPVIAVVGTRKVSSYGRQVTQELVKGLVAHNFIIVSGLALGIDS
                     IAHQTTFENSGYTIAVLGCGLETIYPRSAYPLAKKIISQKGLLLSEFPLGTQPFKQNF
                     PRRNRIIAGLSQATLVIEAGQKSGALITAYNALEQNREVFAVPGNIFSQQSKGTNHLI
                     KKGARLVESVSDIIEALDIGFLNNYIERQPQTVLTPDEEGLMKFLSKEPLQINKLIQL
                     TGLSSAQIMTTVTNLELKGRVKNLGGSNYVIIY"
     gene            5534..6835
                     /locus_tag="VGA49_03565"
     CDS             5534..6835
                     /locus_tag="VGA49_03565"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX9664872.1"
                     /translation="MTQKQLNRHDIIKRLIRKEINGTMAACLMHLSVRQTKRLKAKVL
                     RYGPSALQHGNKGQSGHNKIPAKEEREIIKLLHKHYYDFGPTFASEKLEENHGVKRDP
                     NTIRRIMIVEKLWKPKNKKKEAMHRSWRQRKDCYGEMLQFDGSYEHWLEDRGGTGEMC
                     LLAAIDDATGKAVKATFDEHEGVLPVMSFWQEYIQENGKPNSIYLDKFSTYSMNHKLA
                     KENSDTLTQFQRALDELWIEPILANSPQAKGRVERLFKTFQDRLIKELRLANISTMKE
                     ANEFLKQYLPKYNAKFAVQPHTKTNLHKQLTKTEKKKLPGILSRQKQRTLQNDYTLSL
                     NKQWFQITKNQPINVYKKDKVIVEERTDNTIQIRLRGKYLNYRILPARPPKQKDNDWV
                     IPKTQEQINQSKAHKPAANHPWKQRGCLKTTVKQKEPGMTF"
BASE COUNT         1970 a         1371 c         1429 g         2103 t
ORIGIN      
        1 aaattttcaa cataattttc atcaccactg taatggacac cacgaagttt agtgacgccg
       61 tcaaccagat tggcgacctc tttgccaaat ttccgttcaa gttctgacaa agcggcgtca
      121 gtgtcctcga ccacatcatg caacagacca gcgataattg tcggctggtc tgctttcatc
      181 tctgccagaa tggtggccac ggctgacggg tgaataaaat aaggctcgcc cgacagcctt
      241 ttttgaccct gatgggcttc atcagcgaat ttaaaggctc ttttaatctc ggcgatttta
      301 accggcagct tattctcttc aattgtttga attaaacgtt tgagagacat aattatttgg
      361 gttttcgatg aattgatgat tttagcatag aataatattt tatgaaatac tagtgaagcg
      421 ttttttagat ttaaaacatg aaagcattaa aacattaaaa catgaaagca aaaggtgctt
      481 taatgttatt gtgtttttat gttttaatgg tttcttcaat ttctttctta aaggcacatt
      541 ctttattgga acatttaact ttgtttttac cgccaaaaac cagaagagaa ccgcattccg
      601 gacatttttc accggtcggt ttttgccaca acgcgaattt acagtcagga tattcggaac
      661 aggagtaaaa gacccggcct tttttacttc gtttctcaac aatttcaccc cggccgcatt
      721 ctggacattt aagaccggtt gatttgacga tcggcttggt gtttttgcag tcaggataat
      781 tggagcaagc caggaatttg ccaaagcggc cggttttaat gaccatcggc gagccgcatt
      841 tatcgcagac ttcgtcggtt gtttccggtt caggcggctg accattttta ccttctccgg
      901 gcaaaggctt ggtattttta cattccggat aattggaaca ggccaggaat ttaccgtagc
      961 gaccgatttt gataaccatt ggcgagccgc atttatcgca gacctggtct gttttttcct
     1021 ccgtaatctc ttcctttttc agttctttgc ctttttccgt gattgttttc ttgaaaggct
     1081 gataaaaatt tttgatcatc ggcgcccagt cttttttacc gcgcgcgatc tggtccaggc
     1141 tttcttccat ttcggccgtg aatttaaggt caacgatttt gggaaaatgg gccaccagca
     1201 ggtcattgac aacttcaccg atgtcagtcg gctggagcct tttgtcttca tttttaatga
     1261 cataatttct gtcctgaata gtgctgatag tcggagcgta agttgacggc cggccaatgc
     1321 cttcttcttc cagcgctttg accaggccgg cttcgttata gcgggctggc ggttcggtaa
     1381 agtgctggct gggaattaat ttgattaaat ccagaatttc tttttctgcc agcagaggta
     1441 atagtttttc tttggcttgg gccggataga tttttaaaaa gccgtcaaat ttgatgactg
     1501 aaccagttgc ccggaaaata taatttttag ctttgatatc aactgaagtg ttgtccataa
     1561 tggccggatt catctggcag gcgattgtcc ggcgccagat caaatcatac agccgaaact
     1621 gatttttatc caaatattgt ttgactgatt caggcgtttt attcggaaaa gtcggccgga
     1681 tagcctcatg ggcttcctgg gcgcctttgg cggtggtttt atagaaaacc ggtttgcctg
     1741 gcagatattg atcaccaaat tgttggctga ttaccgctct ggtgtttttg acagcttcag
     1801 ccgacagatt cagggaatct gttctcatat aagtgattaa gccgactgaa ccttctgacc
     1861 caattgagat tccttcatag agctgctgag ccaggaccat tgtttgtttg gtggaaaaac
     1921 ctaatctgtt ggccgcttcc tgctgcaaag tgctggtttt aaagggaggg tttggttttc
     1981 tgattttttc ttgtttggcg attttatcaa cctgatagtc agctttttct aaatctttca
     2041 gaatttgact ggccgctgcc tgatttttaa cggcaaattt atcaagattt ttatcacttt
     2101 gtttaaccag cttagccaga aatgattcag cgccttcttg tccgccagcg gcggatttct
     2161 taagttctga ttcaatagtc cagtattctt cctgcttaaa ggccttgatt tcccgttccc
     2221 ggtcaacaat taaacggacg gcgactgact ggacccggcc ggctgacaag ccccgggcga
     2281 cttttttcca taaaagaggg gatagctcgt agccaaccag gcggtcaaga atccggcggg
     2341 cctgctgagc atccaccagg tcaatattaa tatctctggg ttctttgagg gctttttcaa
     2401 tggcgctttt ggtgatttca tgaaaaacaa ttctttgggt tttcttttta tcaattccaa
     2461 tggccttaat taagtgccag gcgattgctt cgccttcccg gtcttcgtca gtcgccagaa
     2521 taattacatc tgattttttt gcggcttctt ttaaggcttt aatcctggtt ttggcttttt
     2581 ccggaataac ataagtcggc tcaaaattat gttcaacgtc aactcccatt tcttttttgg
     2641 gcagatcacg gacatgacca taggaagaca gcactttgta gtccctgcct aaaaatttag
     2701 aaatggtttt ggctttagtg ggtgactcaa cgatgactaa gtttttcatt ttatttaagt
     2761 tattagaaac ttttacggct tttaactatt ttttcttcag gaatccagcg ctggcggagt
     2821 tcgccggcca gataggttga gccggtaatg acaattaaat cattttttcc ggcctggcca
     2881 agagcgtaat ccagagccgc aaaaggattt aaaaaaaatt tgggaactaa tttattttta
     2941 gaaatttttt taacctgcct ggctaatttt ttcaggtcgg ccgccggccg ccgggaaacc
     3001 tgatagcggg tcagtaaaac ctgatcagcc aggggaatta tttttttgag cattctggag
     3061 ctgtcttttg attccgttat tccaataatg agaaataatc tttgttctgt caaatctttt
     3121 aagatattaa ccagactttt tatttttgaa tcattatggg cgatatctat tataacatcg
     3181 ggtttggact gaattttttc agcccgcccg ggcaggcggc cggctagtaa ccctttttta
     3241 ataattgatt ttttaatccc cagtttattg gctaaattga ttgccagcaa ggcattttta
     3301 agctgatgcc ggccacccat ggccagctga taaatttcat tttcaaattc aaattcagct
     3361 ttatcaaaat cattttttat taatttcgtc gcggatttaa tctgattgaa ttttgccttt
     3421 ttctgacggc aaaccgatct aaaaatatta agaatagccg gccgggtttc agttgtcagg
     3481 aaaattgaat ttggtttaat aatgccggct ttggtctcgg cgattttttt cagaaccttg
     3541 cctaaaatat gagtgtggtc atgattgacg ttggtgataa tcgtaaattt tggcttttga
     3601 ataatatttg ttggatcaaa ttctccgccg cagccaactt ccaggataac atagtcgcat
     3661 ttttcctttt taaaatacaa aaaggcggcc gccagcaaaa tctcaaaata agacggccgg
     3721 ccataactgg ttttcaaata aacttttttg atcggttcag acaagccggc aatgatggcg
     3781 ctaaaatttt ttgttgaaat ataatggttg ttaattctga ttctttcaat cgttgtcgtt
     3841 ggatggggtg aaaaataagc cccggtttta tagccggcgg ccgttaaaac tgactgaatc
     3901 atggatacgg ttgatccctt gccgctggtc ccgccaacat gaacatattt aaaatcccgg
     3961 tgagggttgc cgattaattt taaaaaatac ctgaatcttt tgagattcag ccggcggctt
     4021 ttttggtaat catcagtaat ctggttggga agattatcca gcgattccaa aaattttatt
     4081 gtttgaaaat aattttgaaa cataaaatcc ccttatttcc gtttatctcc cttgatcaac
     4141 cctgatctcc cctgattcta gtatattatc acataatttg aaccgcctaa atttttaacc
     4201 cggcctttta gttcaagatt agtgacggtt gtcatgattt gggccgagga cagtccggtc
     4261 agttgaatca gcttattgat ctgaagcggt tctttggaca gaaacttcat taaaccctct
     4321 tcatcgggtg ttagaactgt ttgaggctgc ctctcaatat aattatttaa aaagccgatg
     4381 tcaagagctt ctataatatc actaacggac tcaaccagcc gggcgccttt tttaattaaa
     4441 tgattagtcc cttttgactg ctgactgaaa atattaccgg gcaccgcgaa aacttcccgg
     4501 ttttgttcca aagcgttata ggcggtaatc agggcccctg atttttgacc ggcttcaatc
     4561 accagagtgg cctgacttaa gccggcaata atcctgtttc gcctgggaaa attttgttta
     4621 aaaggctggg ttcccaaagg aaattctgac aataaaagtc ctttttggct gataattttt
     4681 ttggcgagcg ggtaagccga ccggggataa atagtttcca ggccgcagcc taaaacagct
     4741 atggtatagc cgctattttc aaaagtcgtt tgatgagcga tggagtcaat tcccaaagcc
     4801 aggccgctga caataataaa gttgtgggcg actaatccct taacaagttc ctgagttacc
     4861 tgccggccgt aggaactgac tttcctggtg ccaacgacag caatgactgg cagttgattt
     4921 aatattctaa tattgccctg ataatagagc aggggcggcg gcgcgtagat ttctttgagc
     4981 agagcggaat aatttttgtt ttcaatgtta atcagggaga ttttatgttt ttggattttc
     5041 acaagttctt gttcaggatc aatcatttgt ctttgattaa taatttctct ggctaatttt
     5101 tctcccaggc cggccctaat gaaatcgtca aaagaacccc gccaggcggc ttcaattgat
     5161 ttaaaatatt ttaaaatttt tctggtatta accgggccaa ggttttctat cagggaaaag
     5221 ccgaggtagt gttttgattc agaattaagg cagtttgaca tatcaggtat ttaagaagtt
     5281 aggttagggt taggaagccg gtatagaagt tgggtaaccc taaccttatt tccaaattaa
     5341 aaaacacccc tctcggccaa gccaagctgg gggtggaaat ctggtttaag gatataattg
     5401 tatgtttcaa aagtcaacta ttctgtcgca cggaagtgat aatgtcatag tacaaggaaa
     5461 ttaaaatgtc atacctaata tataatgaaa tggttaatca ttaaccattt caagtatgac
     5521 actagaattt actatgactc aaaaacagtt gaatcgccat gatattatca agcgtttaat
     5581 cagaaaagaa atcaatggaa caatggcagc ctgtcttatg catttgtcag tcagacaaac
     5641 taaaagactc aaagcaaagg tattgcggta tggtccgtca gctttgcaac atggcaataa
     5701 aggccaatcc ggccataaca agatacccgc caaagaagag cgcgaaatca ttaaactatt
     5761 acataaacac tactatgact tcggaccaac ctttgctagt gaaaaattag aggaaaatca
     5821 tggtgtcaag cgagatccaa ataccatacg caggataatg atagttgaaa aattatggaa
     5881 gccaaaaaac aagaaaaagg aagctatgca tcgctcttgg cgacaaagaa aggactgcta
     5941 tggtgaaatg cttcagtttg atggttctta tgagcattgg cttgaagaca gaggaggtac
     6001 cggtgagatg tgcttactgg cagccattga tgacgccacc ggaaaggcag taaaagctac
     6061 atttgatgaa cacgaaggag tcctgccggt tatgtcgttt tggcaggaat atatacagga
     6121 aaacggcaaa ccaaattcaa tttacttaga taaattcagt acatacagca tgaatcacaa
     6181 attagccaaa gaaaacagcg acaccttaac tcagtttcaa agagccttgg atgaattatg
     6241 gattgaacca atattggcaa acagcccaca agccaaaggt agagtggaaa ggctatttaa
     6301 aacatttcaa gacaggctta tcaaagaatt aagattggcc aatatttcaa caatgaagga
     6361 agcaaatgaa tttttaaaac aatatctgcc gaaatacaat gccaaatttg ctgttcaacc
     6421 acatacaaaa actaacttgc ataaacaatt aactaaaaca gagaaaaaga aattgccggg
     6481 catattatcc agacaaaaac aacggactct acaaaatgac tatacattga gtttaaacaa
     6541 acaatggttt cagataacaa aaaatcaacc aattaacgtt tacaaaaaag ataaagtaat
     6601 agtggaagaa agaacagata ataccattca aatcagatta agaggtaagt atctcaatta
     6661 cagaattttg cctgccagac cacccaagca aaaagataac gactgggtga taccaaaaac
     6721 tcaagaacaa ataaatcaat caaaggctca taagcctgcc gccaaccacc catggaaaca
     6781 aagaggttgc ctcaagacaa cagtgaagca aaaagaacct ggtatgacat tttaaaatac
     6841 ttgaaactat gacattttaa cttcctgttg aca
//
LOCUS       DASWHZ010000178         6619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3299489, whole genome shotgun sequence.
ACCESSION   DASWHZ010000178 DASWHZ010000000
VERSION     DASWHZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299489"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VGA49_03570"
     CDS             complement(<1..764)
                     /locus_tag="VGA49_03570"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX9664873.1"
                     /translation="MSTPMLQQYSDIKKNHPDAILFFRLGDFYEMFGEDAIEASKILN
                     ITLTARQKGTDNELPMCGVPHHSADGYLAKLTKAGKKVAICEQLSDPSLPGIVKRDVV
                     RIITPGTTLDNNILNNKKNNYLVSIYRQGDVWGLALADLTTGQFQLTEMTNQAFLENQ
                     IFLINPSEIILTPGLLKSGEAAELIRDFNYNTFLPPTYKSPEELLVSHFKVHSLDGFG
                     ISSYPVGIEAAANLLSYLKETQKTTLDHLNKVSLYN"
     gene            complement(883..1587)
                     /locus_tag="VGA49_03575"
     CDS             complement(883..1587)
                     /locus_tag="VGA49_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664874.1"
                     /translation="MKNIYKIIIIILVAVIIIWGGFLLYFSFLKPEPAAVNRNENLNL
                     NLNLNENENINVNAPVEEVPEEMTEEPVGSADPAAPSNDGAEPTADLPPLEENQVYVY
                     YLNLVAFVEEGEEYIPLVRTKDDPAEADEVFILKQLYWGQLTDEEKEQHGGSLTVEKS
                     GTTGFRRVETRNRVSHVYLTGECDSGGSTLTIANIIFKNLKALDHLDYVKIYDQNEET
                     EEPEGLSDSIPFCLEP"
     gene            1634..2647
                     /gene="ruvB"
                     /locus_tag="VGA49_03580"
     CDS             1634..2647
                     /gene="ruvB"
                     /locus_tag="VGA49_03580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX9664875.1"
                     /translation="MTDQDNNRIIAPSARTDDLMLDNQLRPHRLADFIGQEAIKENLK
                     IFIEAARQRGEPIDHVLLYGGPGLGKTTLSHIIAREMNVNIKITAGPAIERAGDLAAI
                     LTNLQEGDILFIDELHRLNKTIEEILYPAMEDYALDIIIGKGPSARTLRLELPKFTLV
                     GATTRLSLISSPLRDRFGNVLHLNFYQDDHIEKIINRSARIIDVKIDQPAADELARRS
                     RKTPRVANRLLKRVRDYAQVRADGLINLDIAREALAMLQIDHSGLDQTDQKIIETIVS
                     KFNGGPVGLNTIAAYIGEETATIEDVYEPFLIQLGMIDRTARGRVATKTAYDHLGVKP
                     TLL"
     gene            2644..2913
                     /locus_tag="VGA49_03585"
     CDS             2644..2913
                     /locus_tag="VGA49_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664876.1"
                     /translation="MIPLSIFFIIYAIFLLIFLIFAFFSLYHIFKFGFWDFFTYLVTF
                     IFIGGTVIILFITWQSARQIDWSQSLVIFETSQPGSNSLDGLLNP"
     gene            2919..3461
                     /locus_tag="VGA49_03590"
     CDS             2919..3461
                     /locus_tag="VGA49_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664877.1"
                     /translation="MTLENKFPKKAKDEKFILILRRHWFVFFYNTLFYALLGLIPVGL
                     YFLFKTFSPEIFTNQNTRALIILGAGAYYLFVWGFYYIYFVNHILDVWIVTTYRIINI
                     EQKTLFARSWSEQNLDVVQDVTVSVAGFFPTVLGYGDITIQTAGAKIFFIFKQIPDPY
                     KVSKKIIELVEKFKHRNVNN"
     gene            complement(3744..6539)
                     /gene="polA"
                     /locus_tag="VGA49_03595"
                     /pseudo
     CDS             complement(3744..6539)
                     /gene="polA"
                     /locus_tag="VGA49_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
BASE COUNT         1805 a         1544 c         1290 g         1980 t
ORIGIN      
        1 aaattatata aagaaacttt attcagatga tccagggtcg ttttctgggt ttcttttaaa
       61 tacgataata aattagcggc cgcttcaatg ccaacgggat aagagctaat gccaaacccg
      121 tccagactat ggactttaaa atggctgact aacaattcct ccgggctttt ataggttggc
      181 ggcaaaaaag tattataatt aaaatctctg atcagctccg cagcttcgcc tgattttagc
      241 aaaccaggag ttaaaataat ttcagacgga ttgattaaaa aaatttgatt ttccagaaaa
      301 gcctgattag tcatttcagt cagctgaaac tgaccggttg tcagatccgc caaagccagt
      361 ccccagacat cgccctggcg gtaaatggat accaggtaat tatttttttt gttgtttaaa
      421 atgttgttgt ccagcgttgt cccgggagtg ataattctaa ccacgtctct tttgacaatt
      481 cccggcaatg acggatcaga cagctgttcg caaatcgcca cttttttacc ggctttggtc
      541 agcttggcca gatagccgtc agcgctgtga tggggcacgc cgcacattgg cagttcattg
      601 tcagttcctt tctgccgggc ggtcagagtt atgtttaata ttttggaagc ctcaatcgcg
      661 tcctcaccaa acatttcata aaaatcaccc agacgaaaaa acaaaatcgc gtccgggtga
      721 ttttttttaa tatcgctgta ttgctggagc attggggtgg acatagggtg gtatatactt
      781 taacaaaaaa tagccaacag tcaacagtca gctgtcaaca gggaaaaatt taaattgcat
      841 aatccctgtt agctgttggc tgttgactgt tgacttataa tattaaggct ccagacaaaa
      901 cgggatggaa tcgctcaagc cttcgggctc ttcagtttct tcgttttgat cataaatctt
      961 gacataatca agatgatcca aagcttttaa atttttaaaa attatattgg cgatcgtcag
     1021 ggttgaaccg ccggaatcac actccccggt cagataaaca tggctgactc ggttccgggt
     1081 ctcaacccgc cgaaacccgg tcgtgccgct tttttcaact gtcagcgaac cgccgtgttg
     1141 ttctttttcc tcgtcagtca gctgacccca gtaaagctgt tttaaaataa aaacctcgtc
     1201 agcttctgcc gggtcgtctt tggttctgac cagcggaata tattcttcac cctcctccac
     1261 gaaagccacc agattgagat aataaacata aacctgattt tcttccaacg gcggcaaatc
     1321 agctgtcggc tccgccccgt catttgacgg ggcagccggg tcggctgacc cgaccggctc
     1381 ttccgtcatt tcttccggaa cttcttcaac cggggcatta acattaatgt tttcattctc
     1441 attcaagttt aaatttaaat ttaaattttc attgcggtta accgccgctg gttccggctt
     1501 taaaaaactg aaatacaaca aaaatcctcc ccaaattatt atcacggcga caagaataat
     1561 aatgattatt ttataaatat ttttcatatt attatagttt aacatatttt cagataagta
     1621 gtataataga gccatgactg accaggacaa taaccgaata atcgctccat cagccaggac
     1681 tgacgatcta atgctggata atcagctccg gccccaccgg ctggctgatt ttattggcca
     1741 ggaagccatt aaagaaaatc tcaaaatctt tattgaagcg gcccggcaga gaggcgagcc
     1801 aattgaccat gttctgctct acggcggccc gggtctgggc aaaaccaccc tgtcgcatat
     1861 tattgcccgg gaaatgaacg ttaatattaa aatcaccgcc gggccggcca ttgaacgagc
     1921 cggcgacctg gccgccattt taaccaatct tcaggaaggc gatatattat ttattgacga
     1981 gctccatcgg ctcaacaaaa ctattgaaga aattctttac ccggccatgg aagattacgc
     2041 cctggacatt attatcggca aaggcccgtc ggccagaact ttaagactag aactgccaaa
     2101 gtttacctta gtcggcgcca ccacccgttt aagcctgatt tcatcgcctc tgcgcgaccg
     2161 gtttggtaat gtcctgcatt taaactttta tcaggacgat catattgaaa aaattattaa
     2221 ccggtcagcc agaattattg atgttaagat tgatcaaccg gcggctgacg agctggccag
     2281 acgctcccgc aagactcccc gggtggccaa ccgcctgcta aaacgcgtcc gggattacgc
     2341 ccaggtcaga gctgatggtc tgattaattt agacattgcc cgggaagcgc tggccatgct
     2401 ccaaattgac cacagcggcc tggatcagac tgaccagaaa attattgaaa caattgtcag
     2461 taaatttaac ggcggaccgg tcggtctcaa cacgatcgcc gcttacattg gcgaggagac
     2521 agccaccatt gaagatgttt acgagccgtt tttaatccag ctcggtatga ttgatcggac
     2581 ggcccgcggc cgggtggcca ccaaaaccgc ttacgaccat ttaggagtta aaccgaccct
     2641 gctatgattc ctttaagcat tttttttata atctacgcta tttttttgct gattttttta
     2701 atttttgcct tttttagttt gtatcatatt ttcaaattcg gtttttggga ttttttcact
     2761 tatttagtaa cttttatttt tatcggcggg actgtcatta tcttattcat cacctggcag
     2821 tccgccaggc agattgactg gagccaaagc ttggtaatct ttgagaccag ccagccggga
     2881 tcaaacagcc tggacggcct gcttaacccc taaagttcat gacgctggaa aacaaatttc
     2941 ccaaaaaagc caaagacgaa aaattcatct taattttaag acggcactgg tttgtttttt
     3001 tctataatac tttattttac gccctgctgg gactgattcc ggtcggcctt tattttttat
     3061 ttaaaacttt ttcgccggaa attttcacta atcaaaacac ccgggctttg attatcctgg
     3121 gcgccggcgc ttactattta tttgtctggg gattttatta catttatttt gtcaatcata
     3181 tcctggatgt ctggatcgtg accacttacc ggatcatcaa tattgaacag aaaactttgt
     3241 ttgcccggtc ctggtcagaa caaaacctgg atgtcgtcca ggacgtgaca gtcagcgtgg
     3301 ccggcttttt cccgacagtt ctgggttacg gcgacatcac cattcagacg gccggcgcta
     3361 aaatattttt tatctttaaa cagatccctg atccgtacaa ggtctcgaaa aaaatcattg
     3421 aattggtgga aaaattcaaa cacagaaacg tgaataatta ataaacaaaa aaagctttgc
     3481 ggcttttttt attactcatt gaattagatt taagttgtca gccagcccgc cccgcttcgc
     3541 ggagcgaggc cggggaggct gatccacctt tggtggataa gttgtaagat ttaagttgac
     3601 agataggagt ttttgattaa aaatactaaa accctcaatc taataagcaa gacatgacgt
     3661 gatatgaaga atgttcgaaa atgttatttg gtttagaaaa agctttagaa agaaaaaaaa
     3721 cttaaatctt acaacttaaa tctttaatct gtttccgcca gttctcccca actatctcca
     3781 actgaaacat taaccaccac cggcactttc aaaggatagg ctttctccat ctcttcctta
     3841 atcagtttcg cgactgattt tattttattt tccttaactt caaaaaccag ctcatcatgg
     3901 acctgaataa gcattctggc aaaaaaaccc gtctcattcc ctctgattcc ctctaattcc
     3961 tttcctaaat ttatcatcgc tattttaatc atatcggccg ccgtcccctg aatcgggtga
     4021 ttgatggccg cccgctcggc gcctgacctg atcatggcca cgcccgaatt gatttccggc
     4081 agatagcgcg gccggcccag caaagtttca acataaccgt tttcagcggc gaattctttg
     4141 gtgtcttcaa tatatttttt cacgccctga tgaatttcaa aatatttatc aataaattct
     4201 ttggcttttt cccgggaaat gccggcccgc tgggacaagc cgtaaacgcc catgccgtaa
     4261 gtaattccaa aatttatttc tttggccgcc cgacgcatgt catagtcaac ttcttttgga
     4321 tcaactcccc aaatatcggc cgccgtccgg gtatgaatat cctcgccttt tttaaaagat
     4381 tcaatcatcg cccggtcgtt ggcgatggaa gcgacaatcc ttagttcaat ctgggaataa
     4441 tcagctgata aaattttgta gcctttgggg gcgacaaacg cttttctgat ctcccggccc
     4501 agttcggttc tgatcggaat attctgcaga ttgggatcag acgacgataa ccggccggtg
     4561 gcggccactg tctggttaaa agaagtatga attcttctgg tgtcaggatt aaccagcaac
     4621 ggcaaagcgt caagataagt tgacttgagc ttggccagtt cccggtgttc aaaaatcaaa
     4681 tcaataatcg ggtgtttccc ctgcaatttt tccagttcgg cggcggccgt tgacagaccg
     4741 gtttttgttt ttttaatgtc tttagtctct attttcagtt tatcaaacaa aatttctttg
     4801 agctgctggg gggaattaag attaaattcc tgcccagcca gcttgtaaat gtctttggat
     4861 atttttttca gccgctggtc aactttcctg gacatgacga tcaaaaattt cgggtcaatc
     4921 ataataccgt tggtttccat ctccactaaa accggcacca gctccatttc aatttcgtca
     4981 aaaagtttta ataactttct ttccttcagc tctgttttta actttttata aagccgccaa
     5041 gtaaaatcag cgtcttcaca ggaatattcg gacattttct caactggtat ctgttctact
     5101 tttattttat ctttgcctct gcccagcaaa gagtccagcg aaatcttttc atgaccgaat
     5161 tcagtaaaag ccaaaacgtc caaactgtgc tgacggctgc cggggttcag caaataagaa
     5221 gcaatcatcg tgtcaaaata aatgcctttt aaattaacgc cggctttggc caaaacttca
     5281 atatcatatt taatattctg cccgatcttt ttaacggtct catcctccaa aaaaactttg
     5341 agcttatcca aaaaagccct gttgactgtt gactgctgac tgctgactgt aatgtaataa
     5401 gcttctcctt cagcccagct aaaactgacc cccaataatt ttgcccggaa tggatcaatg
     5461 ctggtggttt cagtgtcaac acaaaccagt ttttgagtaa tcaatttttt ataaaaggcg
     5521 tcaaattctt tttgactgtc aattaaatta taactgaacc tttcaccggc tgatattttt
     5581 ttaggctggc taaagaacaa ctcggcctga gtcacctggg gcaattcttt gacagtcgga
     5641 atttttgcca acaatgattt aaactgcatt tcctgaaaaa acttaacaac ctcttcccgg
     5701 ctgaactggc cgtatccaca atcttccaaa ttaaatttaa tcggcacgtc cctgacaatg
     5761 gtcaccaact ctttactcaa tcgcgcttca tcttcatgct caacaatcaa attcactact
     5821 ctttctttaa tccccctgat ctcccctgat ctcccctgat ctccctttat ctcccttaac
     5881 agttcttcta atgatccgaa ctgctttata acttctgtcg ctgtcttctg gccaatacca
     5941 ggaacaccag gaatgttatc agacggatcg cctttgagcg ctttaaaatc atttaactgg
     6001 tcaggtttta aaccataacg gtcaacgacg gcattttcat cataaataaa aatatcgctg
     6061 atcccctttt tcagagtata aacatgagta ttgtcatcaa ccagctgtaa ggtgtccaga
     6121 tcgccggtaa caattaccga taaaacatcc ggcgcgttaa ctgacttttt ttcaaccaaa
     6181 gtcccgatta aatcatcagc ttcgtagccc tcctggccgt aatatttaat gttaaaagca
     6241 tccagcaatt gtttgatccg gtaaatttga gcgtagagct cgtcttattt tctctcgcgg
     6301 gtagattttt attctttgta aactttatgc cgaaaagtcg gcccgggcag atcaaaagcg
     6361 actgtaatat attctggttt atattctttt aaaactttca gcaaaattga agcaaagcca
     6421 taaacggcgt tcacgatttc cccctctttg gtcgtcagag tcggcggcaa agcgtgccag
     6481 gcccggtgaa gcaaagcgtt gccgtcaatt ataataaatt ttttttgttt agcggacata
     6541 aatattcatt aaacttgtat taagcatatc atttatgaac gaggcgtgaa agcataaggg
     6601 ttatcaggga ttatcaggg
//
LOCUS       DASWHZ010000179         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_704682, whole genome shotgun sequence.
ACCESSION   DASWHZ010000179 DASWHZ010000000
VERSION     DASWHZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704682"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VGA49_03600"
     CDS             complement(<1..465)
                     /locus_tag="VGA49_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664878.1"
                     /translation="MPTEISQAILSAITYFDIFDYPLTSFEIEKWLYANNTRMSANQT
                     RIRQVLETELAGPISFDRGFYFLKGREQIVQTRLDRYNLADKKFKRALLAGKLLAALP
                     FVRMIAVCNSLGYGNASDQSDIDFFIITTPGRIWLVRFLSVLIMKILGWRPTA"
     gene            complement(864..3686)
                     /gene="uvrA"
                     /locus_tag="VGA49_03605"
     CDS             complement(864..3686)
                     /gene="uvrA"
                     /locus_tag="VGA49_03605"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9664879.1"
                     /translation="MSEKIIIKGARVHNLKNIDLEIPKNKFVVITGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGLMDKPDVDSIDGLSPTIAIDQRSVSHNPRSTVGTV
                     TEIYDYLRILFAKVGQPHCPNCGQEIFKQTLDQIVSRVAGLADGKQYLILAPVIIDQK
                     GEHRHTLEEIKKSGYHQVRLDGRIYGLEEVLDMSIDKSKKHTLEIIINQPLAELDKSQ
                     LVELLKNALDLGNGFITLLDPVSAGELFCSQYYACRQCDFNLPLIEPRSFSFNSPQGA
                     CPNCTGLGTKLVIDPGLVIPNKKLSLAQGAIKPWLRIFANQKNKIKVLEKVADQHQFT
                     VDQSVSELTDKQLRVIFYGTGSDLFEGVIPELEEKHRETDSDYVRKEIERYMRVLICP
                     ACRGKRLKPEMLAVTVADFSIADLVNLPTAELIEVFQEFSRTKNSQSLKKNKYVKKVL
                     SDRDQKISRQIFKEIHDRLVNLNNVGLDYLTLDRPSTTLSGGESQRIRLATQISSLLT
                     GIIYVLDEPSIGLHPRDNEKLIETLKKLRDLGNTIIVVEHDQTTIVSADAVIDMGPGA
                     GENGGRIIAKGSPEELMKNKESLTGSYLSGQAFIEAPKKTRPGNKKSLIIRGAKAFNL
                     KNIDVEIPLGKFICITGVSGSGKSTLMTDILAKALNHHFYRAKDLPAGHDKIEGLENI
                     DKVVDIDQSPIGRTPRSNPATYTGVFTNIRDLFIEVPEARIKGYKAGMFSFNVKGGRC
                     ENCQGEGMVKIAMQFLQDVYVECEDCHGQRYNKKVLEIFYNGKNIADILNMTVEEARN
                     FFRDVSVIYDKLNTLYEVGLGYIKLGQSATTLSGGEAQRIKLATELSRRATGKTLYIL
                     DEPTTGLHFEDIKRLLLVLNKLVDKGNTILVIEHNLDVIKSSDWIIDLGPEGGKDGGQ
                     LVFSGPPKDIVRVKQSYTGQYLKRII"
     gene            complement(3777..3917)
                     /locus_tag="VGA49_03610"
     CDS             complement(3777..3917)
                     /locus_tag="VGA49_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664880.1"
                     /translation="MDQDRGFKPAPEKKWPRYLLMAGLIVFSVYLFIVLILFLINYFA
                     LS"
     gene            4062..4289
                     /locus_tag="VGA49_03615"
     CDS             4062..4289
                     /locus_tag="VGA49_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664881.1"
                     /translation="MPNKNHLHRLEEKAEKKTQKRAKKDKPKMKVSGKSVLKLKEIIG
                     KAQGTRHEVPPLASGGTRHKARQKGTGNSCA"
     gene            4407..4622
                     /gene="secG"
                     /locus_tag="VGA49_03620"
     CDS             4407..4622
                     /gene="secG"
                     /locus_tag="VGA49_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9664882.1"
                     /translation="MELTLNLVQIIASILLIIVVLMQSQGSGLGGVFGGGGAVYRTKR
                     GVEKLFFYSTIVLAVIFLLSALLQTII"
     gene            4642..>5061
                     /locus_tag="VGA49_03625"
     CDS             4642..>5061
                     /locus_tag="VGA49_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664883.1"
                     /translation="MKSLQKLKNFILAKLKKTALFKKPQNPFEEQTEIDQKLVFGLRK
                     KGGLPSFKQLKQLPKTLSKKEAVWLKIFGWLIIFSLLFSATSWYWQNVEIVPRAGGDY
                     TEGIVGAPVYINPILAQTNDVDLDLSRLIFSSLMKYDK"
BASE COUNT         1400 a         1192 c         1055 g         1414 t
ORIGIN      
        1 ggcggtcggc cgccagccca aaatcttcat tattaaaact gacagaaacc tgaccagcca
       61 gattcttccc ggcgtagtaa taataaaaaa atctatatcg ctttgatcag aggcgttgcc
      121 gtagcccaga ctgttacaaa cagcgatcat ccggacaaag ggtaaagcgg ccagcagttt
      181 gcccgccaat aaagctcttt taaatttctt atcagccaga ttatagcggt ccagccgggt
      241 ctgaacgatt tgttctctcc ctttcaaaaa ataaaacccc cggtcaaagc taatcgggcc
      301 ggctaattcc gtttccagaa cctgtctaat tcgcgtttga ttggctgaca ttcgggtatt
      361 attggcgtat aaccattttt caatttcaaa agaagtcaaa ggataatcaa aaatatcaaa
      421 ataggtgatg gcggataaaa ttgcctggct aatttcagtc ggcatattcc cctattttat
      481 acaaaaaccc acgacttgac aagaattttc tgccatgata taaatagttt gttctttaga
      541 tatactgcgg ttgagcggca acgaaggctt tccccctttt ccttcgttgc cgctcctctt
      601 aatattgtgt ggagaagctg gtttgtcttc ctcgcctctc taggtcatgc cattggacct
      661 cagtcttcag gcgacagacg actggcttct ccgctccccg cccaaaataa atgggcccgg
      721 ggtggattgt ttgactaacc atcaaatgat ccacccctct tttttttagg gtatattagg
      781 gtttattaag gtggattagg gtggattaag gtttaaaacc ctcatcaacc ctcatttacc
      841 tttatcaacc ctaatcaacc ttattaaatt attcgcttca gatactgccc cgtataagat
      901 tgcttaaccc tgactatatc tttgggcggg ccgctaaaaa ccagctggcc gccgtctttg
      961 ccgccttccg gccccagatc aataatccag tcagaagatt taatgacatc aagattgtgt
     1021 tcaatcacca gaatagtgtt gcctttatca accaatttat ttaaaacaag caataacctt
     1081 ttaatatcct caaaatgcaa accggtggtt ggctcgtcca agatataaag cgtcttgccg
     1141 gtcgcccggc gggacagttc agtcgccagt ttaattcttt gggcttcgcc gcccgataaa
     1201 gtcgtggcgc tctgaccgag cttgatataa cccaggccca cttcgtaaag agtattaagt
     1261 ttatcgtaaa tcactgaaac atccctgaaa aaattccggg cctcttcaac agtcatattc
     1321 aaaatatcag cgatattttt gccgttgtaa aaaatttcca aaactttttt attatagcgc
     1381 tggccgtgac agtcttcaca ctccacgtaa acatcctgca aaaactgcat ggcgatttta
     1441 accatcccct cgccctgaca gttttcacag cggccgccct taacattaaa gctgaacatg
     1501 ccggctttat agcctttgat cctggcttcg ggcacttcaa taaacaaatc ccggatattg
     1561 gtgaaaacgc cggtgtaggt cgccgggttt gaccggggcg tccggccgat tggcgactgg
     1621 tcaatatcaa ccactttatc aatattttcc aggccttcaa ttttatcatg cccggccggc
     1681 agatctttgg cccggtaaaa atgatgattc aaagctttgg ccagaatatc agtcatcaga
     1741 gttgatttgc ctgacccgga aacgccggta atgcagataa atttgcccaa aggaatttcc
     1801 acgtcaatat ttttcagatt aaaagccttg gctcctctga tgatcaggct ttttttattg
     1861 cccggacggg tctttttggg tgcttcaata aaagcctggc ctgacaagta ggaaccggtc
     1921 agcgactcct tatttttcat cagttcttcg ggactgcctt tggcgataat ccggccgccg
     1981 ttctcgccgg cgcccggccc catgtcaatt acggcgtcag ccgagacaat agtggtctga
     2041 tcatgttcaa ccacaataat ggtattgcct aaatctcgta actttttcaa agtttcaatc
     2101 agtttttcat tatcccgggg atggaggccg attgacggct catccaaaac ataaattatc
     2161 ccggttaaca acgaactgat ctgggtagcc agtctgattc gctgggattc accccccgat
     2221 aaagtggttg acggccggtc caaagttaaa tagtccaggc cgacattgtt cagatttacc
     2281 agccggtcat gaatttcttt aaaaatttgc cggcttattt tttgatcacg gtcggacaaa
     2341 acttttttaa catatttatt ttttttgagc gactgactat ttttagtccg gctgaactcc
     2401 tgaaaaacct caattagttc ggcggtcggc aaattgacca gatcggcaat tgaaaaatca
     2461 gccactgtca ccgccagcat ttccggtttt aaccttttcc cccggcaggc cgggcagatt
     2521 aaaaccctca tatagcgctc aatttctttg cggacataat ctgaatcggt ttcccggtgt
     2581 ttttcttcaa gttcgggaat caccccttca aacaaatcgg agccggtacc gtaaaaaatt
     2641 acccgcagct gtttgtcggt cagttcggac accgattgat caaccgtgaa ctggtgttgg
     2701 tcagccactt tttccaaaac tttgatcttg tttttctggt tggcaaaaat ccttaaccag
     2761 ggtttaatcg ctccctgggc cagacttaat ttcttattgg gaataaccag cccggggtca
     2821 atcaccagct tggtgcccag gccggtacag ttgggacagg cgccctgggg agaattgaat
     2881 gaaaaactgc gcggctcaat tagcggcaga ttaaaatcgc actgccggca ggcgtaatat
     2941 tgtgaacaaa acagttcccc ggcgctgacc gggtccagca aagttataaa accattaccc
     3001 aggtcaaggg cgtttttaag tagttcaacc aactgacttt tatccagttc agccaacggc
     3061 tgattgataa taatttccaa agtgtgcttt ttggacttgt caattgacat atccaggact
     3121 tcttccaggc cgtaaatccg tccatccagc ctgacctggt ggtagccgct ttttttgatt
     3181 tcttccaaag tatgccgatg ctcccctttt tgatcaataa tcaccggagc taaaatcaga
     3241 tactgtttgc cgtcagccag cccggccacc cggctgacaa tctgatccag tgtttgttta
     3301 aaaatttcct ggccgcagtt ggggcagtgg ggctggccga ctttggcgaa taaaattctt
     3361 aaataatcat aaatttcagt gaccgtgccg acggttgacc ggggattgtg ggagactgac
     3421 cgctggtcaa tggcaattgt cggcgacaga ccgtcaattg aatcaacatc cggcttatcc
     3481 attaagccca aaaattgccg ggcgtaggcc gataagcttt caacatagcg cctttgccct
     3541 tcggcgtaaa tagtgtcaaa ggccagagat gatttgcccg atccggataa tccggtaatg
     3601 accacaaatt tgtttttggg aatttccaga tcaatattct tcaaattgtg gacacgggcg
     3661 cctttgatga tgattttttc tgacatatta atagggatta tatatcaaaa aaatcttttt
     3721 ggcaagtaaa aaccccggct ctttaaaccg gggctccagg ccagcttgat ttaagcttaa
     3781 cttaaggcaa aataattaat caaaaaaagg attaaaacaa taaacaaata aacactaaaa
     3841 acaatcaggc cggccattaa taaatagcgg ggccactttt tttccggcgc tggcttgaag
     3901 cctctgtcct gatccataaa acatttaatt taaattgaat ttattatata atgatacaag
     3961 ggatatcagg gattatcaag gggaatcaag ggacatcagg taaaaactat ccttgatttc
     4021 ccttgctatc ccctgataat ccctgctaaa ccctgtttat tatgcctaat aaaaaccatc
     4081 ttcaccggct tgaagaaaag gccgaaaaaa aaactcaaaa aagagctaaa aaagacaaac
     4141 ccaaaatgaa agtcagcggc aagtcggttt tgaaattgaa agaaataatt ggaaaggcac
     4201 aaggcacgag gcacgaggtc ccgccgctcg cgagcggcgg gacgaggcac aaggcgaggc
     4261 aaaagggaac gggcaatagt tgtgcctaat ccctatataa taaaagtccc gcctaaggcg
     4321 ggacaccaac cctattgcct attgccttgt gcctattgcc tgtcattgac ttatttccca
     4381 ttttctgata gtctgattaa atatctatgg aattaaccct aaatttagta cagattattg
     4441 cttccattct tttaataatc gtcgtcttaa tgcagtccca gggatctggt ttgggcggtg
     4501 tttttggcgg cggcggcgct gtctaccgca cgaaaagggg agttgaaaaa ttatttttct
     4561 actcaaccat cgttctggcg gtaatctttc tgttaagcgc gttattacaa actatcatct
     4621 agtttgactt aatttaactt tgtgaaaagc cttcaaaaac tgaagaattt tattcttgcc
     4681 aaattaaaaa aaaccgcttt attcaaaaaa ccccagaacc catttgagga acagaccgaa
     4741 attgatcaaa aactggtttt tgggcttaga aaaaaagggg gcctgcccag ttttaaacag
     4801 ctcaaacagc tgcctaaaac tttatccaaa aaagaagcag tctggctcaa aatattcggc
     4861 tggctgatta ttttcagtct gttattcagc gccaccagct ggtattggca aaatgttgaa
     4921 attgttccca gggccggcgg tgactacacc gagggtattg tcggagcgcc agtttatatc
     4981 aatccaatac tggcccagac caatgatgtt gaccttgatc taagccggct gattttctcc
     5041 agcctgatga agtatgacaa a
//
LOCUS       DASWHZ010000180         3428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_332479, whole genome shotgun sequence.
ACCESSION   DASWHZ010000180 DASWHZ010000000
VERSION     DASWHZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332479"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1224)
                     /locus_tag="VGA49_03630"
     CDS             complement(67..1224)
                     /locus_tag="VGA49_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9664884.1"
                     /translation="MPKKNIATIYFLSFVWSVAIALPTFIQSSFIEELFGLRVVGLLI
                     MLSTALTLLAMLGYADFIKRYTHNRVVMLVLAADVFSLIGLISMREKWAAAIFFVIQF
                     ICMHLLVLSMDIFLENQSDNEHTGQIRGLLLTTISMAGLFSPIIAGHVVQYFPYTMVY
                     FISAMILIPTFFILIINRKLLSITVSYRRQRLRQWLGSFRIQQPDLVNIFWLNLMLSV
                     FFSVMIFYAPIYLNQHIGFDWAEIGVLFTIMLIPFVLFQAPAGAIADKFLGEKEIALT
                     GLFLMIASVMAIPSLGTKNFVAWAVILFTGRSGVALLRSMIDSYFFKKIDIVDIDIIN
                     AFRALTPLGYLIGSAGVTLILFFLPFKFIFVALAIVLFFGIRPALKLHDTK"
     gene            complement(1254..2297)
                     /locus_tag="VGA49_03635"
     CDS             complement(1254..2297)
                     /locus_tag="VGA49_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664885.1"
                     /translation="MNFFEKYNLKPMQLVKFVGLALVGIILVLIAFKLIGSTLTPILR
                     NTKLSQAVGLQAPSFSGVADELASGQAGVSFGRDVALDLSVRNIPIPTPTPGPAGDQA
                     EAFEITEYNAQIETRQLAQTCLVITELKVRPDVIFENANEHDQGCNYTFKVEREITPE
                     ILAVIESLNPRDLSENTQTIKRLVDDFTSQIEILERKQATIEATLENAISAYDEITAL
                     ATQVRDVESLARIIESKINIIERLTQERIYVSEQLDRLSRAKTEQLDRLEYTYFYVNV
                     IENKYIDGEQLKDSWKAALRGFVRDINQIVQDISINLLTLLLLILQYVIYFFILLVVA
                     KYGWKLTQYIWKK"
     gene            complement(2543..2998)
                     /locus_tag="VGA49_03640"
     CDS             complement(2543..2998)
                     /locus_tag="VGA49_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664886.1"
                     /translation="MNFKHQTQSDQLDRYSFLWSQARLILAAIALFLGGVPPILAFLP
                     IAGLYGLLSSLLTLAWIISGLAAGYMIYRWNQNNQKIFGGKNQKDTIAFFISVVSGLN
                     LGLAGLSGKNIGMSISSLSIVFILVGILYLATAIYLQQRWNASKQKLFG"
     gene            3068..>3428
                     /locus_tag="VGA49_03645"
     CDS             3068..>3428
                     /locus_tag="VGA49_03645"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9664887.1"
                     /translation="MLNISEQSRRSPKELKAEVLDFLQQHRVAVLSTLTPDNKPYAAP
                     VIYAVDNSFHFYFFTRSDSKKYVGLAKDCLVSLVVYDEQEPQVVQTEGCASLEEDEQA
                     IKEMSAELIKISMDSSPW"
BASE COUNT         1012 a          724 c          829 g          863 t
ORIGIN      
        1 ttttgacata agtatagtat acaaaaaacc aacggtttac gcaggtttta ttgttcagcc
       61 ctcaggttat ttggtgtcgt gtaatttaag cgcgggtcga attccaaaaa ataatacaat
      121 tgccaaagcc acaaaaataa atttgaaagg taagaaaaat aagatcaggg ttacgccggc
      181 tgaaccgata agatatccaa gcggcgttaa cgcccgaaag gcgttaataa tatcaatgtc
      241 gacaatatca atctttttaa agaagtaact gtcaatcata gatctaagta acgccacgcc
      301 ggaccggccg gtaaagagaa tgaccgccca ggccacaaaa tttttcgttc ccaacgatgg
      361 tatcgccatg accgaggcaa tcatcagaaa cagtccggtt aaagcgattt ctttttcgcc
      421 taaaaatttg tcggcaatcg cgccggccgg agcctggaaa agcacaaaag gaatcagcat
      481 aatcgtgaat aaaactccaa tctccgccca atcgaaacca atgtgttgat tcagataaat
      541 tggcgcgtag aaaatcatga ccgaaaaaaa tacggataac ataagattga gccagaaaat
      601 atttacaaga tccggctgct ggattcggaa tgaacccagc cattggcgga ggcgctgacg
      661 gcggtatgac acagtgatgc tgaggagctt tcgattaata attagaataa aaaacgttgg
      721 aattaatatc atggctgaaa tgaagtagac catggtgtag ggaaagtatt ggacaacgtg
      781 tccggctatg ataggcgaaa aaagaccggc catactaatg gtggtcagga gcaagcctcg
      841 gatttgtccg gtgtgttcat tgtctgactg gttttccaaa aagatatcca tacttaacac
      901 cagcagatgc atgcagatga attggataac aaaaaagatt gccgccgccc atttttcccg
      961 catactaatt agaccaatca agctgaagac atctgcggcc aaaacgagca taaccacgcg
     1021 gttatgcgtg tagcgcttaa tgaaatcggc gtaaccgagc atggccagca gcgtcagagc
     1081 ggttgagagc ataattaaca aaccgaccac gcgtaacccg aataattcct caataaatga
     1141 ggattgaatg aaggtcggca gcgcgattgc aaccgaccag acaaacgaga gaaaatagat
     1201 tgtggcaata ttttttttcg gcattcaaac cggtcgcgtt tttccggaac gcgttatttc
     1261 ttccaaatgt actgagtcaa tttccagccg tatttggcta cgaccagcaa aataaagaag
     1321 taaataacat attgcagaat cagcagcagg agtgtaagga ggttaatgga aatgtcttga
     1381 acaatttgat taatgtctcg gacaaagcca cgcagagccg ctttccatga atctttaagc
     1441 tgctcgccgt caatgtattt attttcaatt acgttgacgt agaaatacgt gtactcaagc
     1501 cggtcaagct gttcggtttt ggctcgacta agccggtcaa gctgttcgct aacgtagatc
     1561 cgctcttggg ttaaacgctc aataatatta attttactct caataattcg ggccaggctt
     1621 tcaacatccc gaacctgggt ggccagagcg gtaatttcgt cgtaggcgga aattgcgttt
     1681 tcaagcgtgg cttcaatcgt cgcctgcttg cgctccaaga tttcaatttg gctggtaaag
     1741 tcgtcaacca ggcgttttat ggtctgcgtg ttttcggaaa ggtctctcgg gtttaggctt
     1801 tcgataacgg ccaggatttc aggtgtaatt tcacgctcaa ctttaaacgt gtagttgcag
     1861 ccctggtcat gttcgttggc attttcaaaa ataacatccg ggcgaacttt aagctcggtg
     1921 ataaccaggc aggtttgtgc cagctgacgt gtttcaatct gagcattgta ttcggttatt
     1981 tcaaacgctt cagcttgatc tccggccggg ccgggggtcg gcgtcggtat tggaatgtta
     2041 cgcacagaca agtcaagcgc cacgtctcgg ccaaacgaaa ctccagcctg accggaggcc
     2101 aactcgtctg ccacgcccga aaaactcgga gcctgcaagc cgactgcttg gcttagtttt
     2161 gtgttacgca ggattggggt cagggttgaa ccgataagtt taaaagcgat taagaccaga
     2221 ataatcccga ccagagccag gccgacaaat ttgaccagct gcatcggctt aagattgtat
     2281 ttttcaaaaa agttcatagg taaaattatt tattgttttt atagcactcc agattttttt
     2341 aagaggtatc caatgaagat aactaaaatt aagtagtagg ttgtccagcc gcgcgtgatc
     2401 gggaaatagc gggaaataat tcggaaattc cgtttgtacc atttgtcatc gcccagccag
     2461 gccataaggt tggagccgac actgtcgata agcaaaatgt aaaaaatgat tggttcaatt
     2521 ctgatgttca tcggtctaac atttagccaa agagtttttg cttggaagcg ttccagcgtt
     2581 gctggaggta gattgcggtt gcgaggtaga ggattccaac caaaatgaat acgattgaca
     2641 gcgatgaaat tgacatgcca atatttttgc ccgaaagtcc ggccaggccc aggttaagac
     2701 ctgaaacaac gctgataaaa aacgcaattg tgtccttttg gtttttgccg ccaaaaattt
     2761 tttgattgtt ttgattccag cggtaaatca tgtagccggc cgcgagaccg gaaataatcc
     2821 aggccagcgt cagcagggag cttagcaggc cataaagtcc ggcaattggg agaaatgcca
     2881 gaatcggcgg tacaccgccc agaaacaaag cgatggccgc cagaatgagc ctggcttgcg
     2941 accagaggaa gctgtaacgg tcaagctgat cggactgagt ctgatgtttg aaattcatac
     3001 gtgttggatt agggcttgta agagtctaaa aacttagata tgcttatcat aacataaaaa
     3061 ttgcaacatg cttaatattt ccgaacaaag caggcggtcg ccaaaagaac ttaaggccga
     3121 agtgcttgac tttttgcaac agcatcgggt ggcagtgctt tcgaccttga cgccggataa
     3181 caagccgtac gcggcaccgg ttatttatgc cgttgacaac agcttccact tttatttttt
     3241 tacgcgcagc gacagtaaaa agtatgtcgg cctggctaaa gactgtctgg tttcgctggt
     3301 ggtgtacgac gaacaagagc cgcaggttgt tcaaacggaa ggatgcgcca gccttgagga
     3361 ggatgagcag gcgattaaag agatgagtgc cgagctgatt aaaatatcaa tggattcaag
     3421 cccatggt
//
LOCUS       DASWHZ010000181         4625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3303363, whole genome shotgun sequence.
ACCESSION   DASWHZ010000181 DASWHZ010000000
VERSION     DASWHZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3303363"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="metG"
                     /locus_tag="VGA49_03650"
     CDS             <1..1462
                     /gene="metG"
                     /locus_tag="VGA49_03650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9664888.1"
                     /translation="YYVNDKPHLGHAYTTIAADVLARFKRLQGHDVFFLTGTDEHGVK
                     VSQSAKAARLSPQEFTDQQSAIFRMAWDILDISNNDFIRTTEPRHEEAAKKFLLELFD
                     KNLIYKDKYEGLYCVGCESYKTDSELVDGKCPDHNIEPILLSEDNWFFKLSRFNDQLK
                     KLIEKDELLVRPVARKNEMLGLLKQGLEDIAISRQKVDWGIPLPFDKSQTAYVWIEAL
                     MNYVTGVGFQDDKKRFQQIWPADVHLMAKDIVKFHALIWPAMLLGVGLPLPRQVFAHG
                     FFTVDGKKMSKTLGNVIDPVEISEKYGVDGLKYLIIREISFGEDGDISLGRFDERYNA
                     DLANGLGNLVSRVLAIAGQREFETDYSQSKDFEQQVKLTWAGYEQAMTDLKFNDALDS
                     VWKLISSCDKYIEVKKPWELSGDGLIPVLTNVLEAIRQIGWLIQPFMPLTAEEILSQL
                     KVYDTEKIKPLKDIQAWGGLTGKLALSKGEALFIRL"
     gene            1494..1994
                     /locus_tag="VGA49_03655"
     CDS             1494..1994
                     /locus_tag="VGA49_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9664889.1"
                     /translation="MTLVDVIILIIIFGFVLYGFWFGLIHTIGALLGLVVGSYVAGFS
                     FDIVADSIDFIFGGNTNLARLVSFILILAITRRLVGFLFVFVEKIFKFLQIIPLVKTA
                     NRIAGAAIGLLEGIIVISLVLYLASQYPITPFIEQLLTGSWVAQVFIVLAELFVPILP
                     AAFRGA"
     gene            2070..2939
                     /locus_tag="VGA49_03660"
     CDS             2070..2939
                     /locus_tag="VGA49_03660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9664890.1"
                     /translation="MCPVSKPFLVDTHAHLFFEDYTKDYRQVIQRALDKGIWLIIPGL
                     DLKTSQRAIEIASEFPEGVFAAVGLHPTDILKELNGRQRPDLEPYRELAGQPKVVALG
                     EIGLDYYHLKRIAHEHQISPDDYIQLQRNVLKDFIKLSEEFRLPLIIHCRGVGTEEKV
                     HSDLTNILREFDQSSKGFDTRGVLHCYSGDWEQAVQYLNLDFYISFTGMITYPNFKKQ
                     ELINKIPLEKLLSETDCPFMAPESKRGQRNEPLFVEETVRKLAEIRGDDFETVAKQLT
                     DNAFEMFQFEKHG"
     gene            3160..3327
                     /locus_tag="VGA49_03665"
     CDS             3160..3327
                     /locus_tag="VGA49_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664891.1"
                     /translation="MDNKEKEDQVEKVLESLTGTDRWNLLEELRQTSRKVGLIIRPRK
                     ATGRRRESDWD"
     gene            3396..4232
                     /locus_tag="VGA49_03670"
     CDS             3396..4232
                     /locus_tag="VGA49_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9664892.1"
                     /translation="MYFDTHAHINFKAFKDDSLEVLQKSLDDDIWVVNVGSRYSTSQR
                     AVEFAQKFEEGIYAAVGLHPVHLREIDVVLENGFSFKSRVEEFDYDQYKKLALNEKVV
                     AIGECGLDYAGLDKFGLDIDVVKKFQAKIFRQQIKLATELGLPLIIHCREAEDDLVKV
                     LHDKLKDVQANGAVAHCFMGDEKIAQFYIDNDLYLSFTTLILYNRQWDEIIKRIPLEK
                     ILTETDSPYFLPPAMKGKRNEPVNVKYVVQRIARIKNLDEAWVAEVTFNNALKFFKLS
                     KK"
BASE COUNT         1312 a          864 c         1073 g         1376 t
ORIGIN      
        1 ttattatgtt aatgataagc cgcatttggg gcacgcttat acaactatcg ccgctgatgt
       61 tttagcccgc ttcaaaagac tccagggaca tgatgtcttt tttttgaccg ggacagatga
      121 gcacggagta aaagtttccc aatcagccaa agcggccaga ctgtctcccc aggaatttac
      181 tgatcagcag tcagctattt ttagaatggc ctgggatatt ttagacattt ccaataatga
      241 ttttatccgg acgactgaac cgcggcatga agaagccgcc aagaaatttt tgctggagct
      301 atttgataaa aatttgattt ataaagataa atacgaggga ctttattgcg ttggttgcga
      361 atcttataaa actgacagcg agctggttga cggcaaatgc cccgatcata atattgagcc
      421 gattttattg tcagaagaca actggttttt taaactgtcc cggtttaatg accagcttaa
      481 aaaattgatt gaaaaagatg agcttctagt ccgcccggtt gcccggaaaa atgaaatgct
      541 gggtttgtta aagcagggcc tggaagatat tgccatcagc cggcaaaaag ttgactgggg
      601 cataccgctg ccgtttgata aaagccagac agcttacgtc tggattgagg ctttgatgaa
      661 ttatgtcacc ggagttggct ttcaggatga taaaaaaaga tttcagcaaa tctggccggc
      721 ggatgttcac ttgatggcca aagacattgt taaatttcac gctttaattt ggccagccat
      781 gctgttggga gtcggcttgc ccctgccccg acaagttttt gcccatggtt tttttacggt
      841 tgacggtaaa aaaatgagca agactctggg caatgttatt gatccggtgg agatttctga
      901 aaaatacgga gttgacggtt taaaatattt gattattagg gaaatatctt ttggcgagga
      961 cggcgatatt tctttgggca gatttgatga acgctacaat gctgatttgg ccaacgggct
     1021 tgggaattta gtttcccgcg ttttggccat cgccggccag cgggaatttg aaactgatta
     1081 ttctcaaagt aaagattttg aacaacaggt caaattaacc tgggcggggt atgaacaggc
     1141 gatgactgat ttaaaattta atgacgcctt ggattcagtc tggaaactga tcagttcttg
     1201 cgataaatat attgaagtta aaaaaccctg ggagctgtcg ggcgatgggc taattccggt
     1261 tttgaccaat gtcctggaag caatccgcca gattggctgg ctgatccagc cgtttatgcc
     1321 gctgaccgcc gaggaaattt tgagccagct gaaagtctac gacactgaaa aaataaaacc
     1381 attaaaggat attcaggcct ggggcggact gacaggcaag ctggcattga gtaagggaga
     1441 ggcattgttt attagactat aaattaaaaa ttagtcatta gaaattttaa actatgactt
     1501 tagtagacgt tataattctg attatcatat ttggttttgt cctctatggt ttttggtttg
     1561 gtctgatcca tactatcggc gctttgctgg gcctggtggt tggcagttat gtggccggtt
     1621 ttagttttga tatcgtggct gacagtattg attttatctt tggcggcaat accaatctgg
     1681 cccggctggt ttcttttatt ttaattttag cgataaccag acggctggtc ggctttttgt
     1741 ttgtttttgt cgaaaaaata tttaaatttt tacagataat tccgctggtt aaaaccgcca
     1801 accggattgc cggcgcggcc attggcttat tggaaggcat tattgtcatc agtttggttt
     1861 tgtatctggc cagtcaatat ccgatcaccc cttttattga acagctattg accggttcct
     1921 gggtggccca ggtttttatt gttttggccg agctttttgt gccgatcctg ccggcggctt
     1981 tccggggagc ataaaaatag atttaagatt taagttttga gatttaagtt actttaatct
     2041 ttaatcttaa atctttaatc tgaaattata tgtgtccagt gtccaagccg tttttagtag
     2101 atactcatgc tcatcttttt tttgaagact atactaaaga ttacagacaa gtcattcaaa
     2161 gagctttgga taaaggcatc tggctgatta ttcccggact ggatttgaaa accagccagc
     2221 gggcaattga aatcgccagt gaatttccag agggagtttt cgccgccgtc ggcctgcatc
     2281 caacagatat tttaaaagaa cttaacggcc ggcaaagacc cgatctggag ccgtaccggg
     2341 aattagctgg tcagccaaaa gtcgtcgctt tgggcgagat cggcctggac tattatcatt
     2401 taaaaagaat cgcccatgaa catcagatta gcccggatga ttatattcag ctgcagcgga
     2461 atgttttaaa agattttata aaattgtcag aagaatttcg tttgccgttg attattcatt
     2521 gccggggagt ggggacggaa gagaaagttc attcagacct gactaatatt ttgcgggaat
     2581 ttgaccagtc ttccaaaggt tttgatacgc ggggagtcct gcattgttat tcgggcgact
     2641 gggagcaggc ggttcagtat ttaaatttag atttttatat ttctttcact ggcatgatca
     2701 cttatccaaa ttttaaaaaa caggaattaa tcaataagat tcctttggag aaattattgt
     2761 cagaaaccga ctgtcccttt atggcgcctg aatcaaaacg ggggcaaaga aacgaaccgc
     2821 tatttgtgga ggaaacagtc agaaaactgg ctgaaatcag aggtgatgat tttgaaacgg
     2881 tcgccaaaca gctgactgat aatgcttttg aaatgtttca gtttgagaaa cacggttagg
     2941 attaaggatt aagaaattag atttaaattg tagagcttct taattctatc gcgcaacttt
     3001 agtctgggtt ggtgaaaacc agcttttcat attgacttaa aatttaaaaa agtatatgat
     3061 aacctattcg cttcatcggt ctttagcatc caaggagggc gccgcgtgac tgtactcaat
     3121 gttcgggaca agcttatcag gatcctttat catcctaatt tggacaataa agaaaaagaa
     3181 gatcaggttg aaaaagttct ggaaagcctg accgggactg acaggtggaa tttgctggag
     3241 gagcttcgcc agacttcccg caaagtcggc ctgatcattc ggccccgcaa ggcgaccggc
     3301 cgccgtcgtg aaagcgactg ggactagttc cgccacccgt caggttaatt tctgacggtt
     3361 ttttttccgg agtctgactc cggagtctga cttctatgta tttcgacacc cacgctcaca
     3421 tcaacttcaa agcttttaag gatgattcct tggaagtcct tcaaaaatcc ctggatgatg
     3481 acatctgggt agttaatgtt ggctcgcgat attcaaccag ccagcgagcc gttgagtttg
     3541 cccaaaagtt tgaagagggg atttacgccg ctgtcggttt gcatccggtt catttacggg
     3601 agattgatgt tgttttggaa aacggtttta gttttaaaag ccgggtggag gaatttgatt
     3661 atgatcaata caaaaaatta gctttgaatg aaaaggtggt ggcgatcggg gagtgcggcc
     3721 tggattacgc cggtttggat aaatttggtt tagacataga tgttgttaaa aagtttcagg
     3781 ctaaaatttt tcgtcagcag ataaaactgg ccacagagct tggtttacct ttaattattc
     3841 attgccggga agcggaagac gatctggtca aagtccttca tgataaactt aaagatgttc
     3901 aggccaatgg cgcggtcgct cattgtttta tgggagatga aaaaattgcc caattttata
     3961 ttgataacga tctgtatctg tctttcacca ctttaatttt atacaatcgc cagtgggatg
     4021 aaattattaa aagaattcct ttggagaaaa ttttaactga aactgactcg ccttattttt
     4081 tgccgccggc gatgaaaggt aaaaggaatg aaccagttaa tgttaaatat gttgtccaaa
     4141 gaatcgcccg aatcaagaat cttgatgaag cctgggtggc tgaagtaact tttaataatg
     4201 ccctgaaatt ttttaaactt tccaaaaaat gaaaaaggaa ctagaaagct gaaataagtg
     4261 gttggttgaa ttgagccgac aggctcaaag atcaacatga ttgaagcgcc gggcgcttca
     4321 acaaacactt tttagctaaa tagttccttt cgcggaccga gattgggggt tggggaaatg
     4381 tgttttggta agttataact ctctccagtc taaggactct ctcctcaaaa aaaaatataa
     4441 aataccacag tcatgacttc cccctcaatt aacccccgcc cggggatcct ccctgaattg
     4501 ggagtaccgg cttatcaaca aaatattaac attttatcca gattattgat aaacaggcgc
     4561 tcccaatcaa aataacttag caaatcagct aaaaattgtc aatatttagc caaaacacaa
     4621 aaaga
//
LOCUS       DASWHZ010000182         5941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3304714, whole genome shotgun sequence.
ACCESSION   DASWHZ010000182 DASWHZ010000000
VERSION     DASWHZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304714"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGA49_03675"
     CDS             complement(<1..989)
                     /locus_tag="VGA49_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664893.1"
                     /translation="MPRSNLYKVIVVAVLAGFLVGIIAELIYQVYFVIPSLTTGDVVI
                     NRQLSLESSKKIQSVVQNISPSLVTVYRKKPLREINNPYFADEILGRGLIITNDGWLI
                     AYINSQEAIGSLKNNIIVKTSDASLYNVMAGLEDPATGILFLKIEAANLVPAQFGDSG
                     QLKLSEQIIIAPDTNSARISYLENLNYYHQSNLNQLTLSSEIYSNFLMIQDKPDLRFI
                     GAPVLNLDGEIIGILNKLDENQAALVTPANYFKGLIGNVLAQNKISRPRLGLNYFDLS
                     MVLGLNQQLTHEFKKGALVLNLQKNSPALGLLKTNDIILKVDGEEVTAARGLS"
     gene            complement(1154..1918)
                     /locus_tag="VGA49_03680"
     CDS             complement(1154..1918)
                     /locus_tag="VGA49_03680"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664894.1"
                     /translation="MLLLKRILPVLTPTAVFLGFEYIFVNADSFWRVVVLIYILLILS
                     VIIIVYNKITLSAAWVYLINPILLVSSALLFVILLESSLLRHLILAATVLILGSILNN
                     IFIYLHQTHRYQAYSFESLIDYSNLIVAFLFFSSFYFLTIFLNILIWQLFILAFIIIA
                     LLSYQLIWSNKIDLKRGAVYVFINSLVLSQLFWAISFLPIGYLASGLILTLIFYMISG
                     MSKLTLLDRFSLRKSGKYLIIGFLGIIVVLATTRWL"
     gene            complement(1931..2314)
                     /locus_tag="VGA49_03685"
     CDS             complement(1931..2314)
                     /locus_tag="VGA49_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664895.1"
                     /translation="MLKRFLTIALAFVLFLSLAGLNGCQNKQTSTEQPAPVDLVRLEE
                     DYLTSLTDILTNYQSRSADLTLDLVTKTQSDVLDLVVPGSYKNFHLNLVLSLNAVRQG
                     LELNDQSKVTAGQADLDKLMEQILN"
     gene            complement(2307..3422)
                     /locus_tag="VGA49_03690"
     CDS             complement(2307..3422)
                     /locus_tag="VGA49_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9664896.1"
                     /translation="MRIAIDARFYGPQTKGLGRYIERLTENLEKIDHQNHYLIFLGRD
                     NWDQYQPQNGNFSKVLADFKWYGLAEQLKMPYLLWKHRVDLVHFPHFNVPLLNLKKFV
                     VTIHDLILIQYPTPRATTLSPFLYKIKNFFYKLVIAAAVQRSEKVITVSEYSKSQILK
                     QFKINSEKVVVTYLACDSVESGQLKMPESNLQKYGIDKPYLLYVGNAYPHKNLERLLT
                     AFKKFTEIFGAGYQLVLVGKKDYFYQRLEESCNDDNVIFCGYVSQPVLADLYRNARLY
                     VFPSLLEGFGLPALEAMRYDLPVVASAAASLPEILGQAALYFNPNSIDDMITKMRHCL
                     TDHQTRQNLINLGRIQITKYKWLDCAQKTLLIYQNYA"
     gene            complement(3406..4569)
                     /locus_tag="VGA49_03695"
     CDS             complement(3406..4569)
                     /locus_tag="VGA49_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9664897.1"
                     /translation="MKIGIDIRSLAEKKYSGVSEYSYNLLNNLFRIDQGNQYYLFANA
                     YHQKTLPDFSFENVAVCYSRIPNKLLNFSLKFFKYPKLDKLVAKKFKLEKLDLFFLPN
                     LNFVSLSDDVAKVITVHDLSFERYPKFFNFKKRLWHRLVNPRSLINSADKIISVSLNT
                     KNDLTALYQTDQSKIEVIYPGSRPRKELSSAELTAVKNKHHLPDKFFLYLGTLEPRKN
                     ITGLIKSFDIYKSKYWTTALETYLVLAGSGGWLMNDILEAINASPNKEYIKLPGYVDA
                     EDKAGLYQLADVFIYPSFYEGFGFPPLEAMAAGTPVIASHSSSLAEVLADSCLLADPY
                     NSFELAYLMNYLTTDQELRQDLIERGLEKVKQFSWQKTAAEIKKLFEENANRH"
     gene            complement(4566..5654)
                     /locus_tag="VGA49_03700"
     CDS             complement(4566..5654)
                     /locus_tag="VGA49_03700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9664898.1"
                     /translation="MKVALVHDYLAQDGGAEKVLKVFQEIFPGAPTFVLVHNKDKANP
                     IFLKKDIRTSFIQKMPLGVKKYQWFLPLMPLATEKYNLKGYDLVLSSSSAFSNGVLTG
                     PQTLHICYCHTPTRFLWSDTHEYVQELKYNRLVKKIVPLLLTRLRLWDRQAADRVDRF
                     IANSATVQQRIKKYYRRQSDIINPPVETDIFNIAPATGDYFLTGGRLVSYKRFDITVQ
                     AFSRLGVSLKIFGIGPAMEDLKKIAKDNIEFLGQITDAEKAELYGRALAFIHPQEEDF
                     GITPIESMASGRPVIAYAAGGALETVIAGQTGEFFKEQTWEELADLVIRFDKNRYNPE
                     QIRSHALKYNVDSFKTKIKDYIDKSLLL"
     gene            complement(5681..>5941)
                     /locus_tag="VGA49_03705"
     CDS             complement(5681..>5941)
                     /locus_tag="VGA49_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX9664899.1"
                     /translation="LLGQMSLVGPRPHQPREVARYEKHHKVVLTIKPGITGLAQISGR
                     SDLDFEDEVKLDSFYIESWSLLMDVAILLKTPFSLLRKRKYT"
BASE COUNT         1860 a         1241 c         1125 g         1715 t
ORIGIN      
        1 tcagacaaac cccgggcggc cgttacttct tcaccatcaa cttttaaaat aatgtcattt
       61 gtttttaaca ggcccaaagc ggggctgttt ttttggagat ttaaaaccaa agcgcctttt
      121 ttgaattcat gggtaagctg ctgattcaat cccagaacca ttgataaatc aaagtaattt
      181 agcccgagcc gggggcggct gatcttattt tgagccagga cattgccgat taaaccttta
      241 aagtaattag ccggggtcac taaagcagcc tgattttcat ccaatttatt taaaataccg
      301 ataatctcgc cgtccaggtt taaaactggc gctccaataa atcttagatc cggtttatcc
      361 tgaatcatta aaaagtttga ataaatttca gatgataaag tcagctgatt taaatttgac
      421 tgatgataat aatttaaatt ttccagatag ctaatcctgg cgctattcgt gtcaggcgcg
      481 ataattattt gctctgacag tttaagctgg ccggaatctc caaattgagc tggaactaag
      541 ttggccgctt ctatttttaa aaataaaata ccggtcgccg gatcttccag accggccatc
      601 acattatata aactagcatc gctggttttg acaataatat tatttttcag cgaaccaatt
      661 gcttcctggc tgttgatata agcgatcagc cagccgtcat tggttataat caggccccgg
      721 cccaaaattt catccgcaaa ataaggatta ttaatttcac gcaatggttt ttttcggtaa
      781 actgtcacca aactgggtga aatattctga acgactgact gtatcttttt tgatgattcc
      841 aggcttaact gacgatttat aacaacatcg ccggttgtta atgacggtat tacaaaataa
      901 acctgataaa ttaattcagc gataatgccg actaaaaagc cggctaaaac agcaaccact
      961 ataactttat ataaatttga tcttggcata aaagcatgaa agcattaaag catgaaagca
     1021 agaaagcaag aaaacatgaa agcattaaag catgaaagca agaaagcaag aaaacaaatt
     1081 gttttaatgt tttaatgttc ccttgtttta atgttttaat gttctcttgt tttaatgttt
     1141 taatgttctc cctttagagc cagcgggtgg tggctaaaac cacgattatt cccaaaaacc
     1201 cgattatcag atattttccc gactttctca gcgaaaatct atccaaaagc gttaatttgc
     1261 tcattccaga tatcatataa aaaatcagag ttaaaatcaa gccgctggcc aggtagccga
     1321 ttggcagaaa gctaatcgcc caaaatagct gggacaaaac caggctatta ataaaaacat
     1381 aaaccgctcc ccttttcaga tcaattttat tgctccaaat caattgatag gatagcaaag
     1441 ctataattat aaaagctaaa ataaacaact gccagatcaa tatatttaaa aaaatcgtta
     1501 aaaaataaaa cgagctaaaa aacaaaaaag caactatcaa attgctgtaa tctattaaac
     1561 tttcaaatga ataggcctga tagcgatgag tttgatgaag ataaataaag atattattca
     1621 aaatacttcc aagaattaat acggttgccg ccaatattaa atgacgcaat aaactgctct
     1681 ccagcagaat cacaaacagc agagcgctgg aaaccagcag gatcggattg attaaataaa
     1741 cccaggcggc gctcaaagtg attttattat agacaatgat tatcacgctt agaatcaata
     1801 atatataaat taagaccaca acccgccaaa aactgtcagc atttacaaaa atatattcaa
     1861 aaccaagaaa aaccgccgtg ggcgttaaaa ctggcaggat tcgttttaaa agaagcataa
     1921 agaaatagtc ttagtttaaa atctgttcca ttaacttgtc caaatcagcc tgaccagcgg
     1981 tgacttttga ctgatcgttg agttccaaac cctgcctgac ggcgttgaga cttaaaacca
     2041 ggttcagatg aaaattttta tagctgcccg ggacgaccaa atccaaaaca tcactctggg
     2101 ttttggtcac caagtcaagg gttagatcag ctgatcgcga ctgataatta gttaaaatat
     2161 ccgttaacga cgttaaataa tcctcttcca gcctgactaa atcaaccggc gccggttgtt
     2221 cagtgctggt ttgtttattt tgacaaccgt ttaaaccggc cagacttaga aataaaacaa
     2281 acgccagggc gattgtcaaa aatcttttaa gcatagtttt gataaattaa taatgttttt
     2341 tgggcgcaat caagccactt atatttagtt atttgaatcc ggccaagatt aattaaattt
     2401 tgccgggtct ggtgatcggt caaacaatga cgcatttttg ttatcatatc atcaatgctg
     2461 tttggattaa agtaaagagc cgcctgaccg agaatttccg gcaaactggc ggcggctgaa
     2521 gcaacaaccg gtaaatcata ccgcatcgct tccagggccg gcagaccaaa gccttccagc
     2581 aatgacggga aaacatacag ccgggcgttt ctgtataaat cagccaaaac cggctggctg
     2641 acatagccgc aaaaaattac attatcgtca ttacaagatt cttccagtcg ttgataaaaa
     2701 taatcttttt taccaaccaa aaccagctga taaccagctc cgaaaatttc agtgaatttt
     2761 ttaaaagcgg tcagcagacg ctccagattt ttatggggat aagcgttgcc gacatatagt
     2821 aaatagggct tatcaatgcc gtatttttgt aaatttgatt ccggcatttt cagctggccg
     2881 ctttccacgc tgtcgcaggc cagataagtg acaaccactt tttcagaatt gatcttaaac
     2941 tgtttcaaaa tttgactttt actgtattct gaaacagtta taaccttttc tgatcgctga
     3001 acggccgcgg caataaccag tttataaaaa aaattcttta ttttatataa aaacgggctc
     3061 aaagtcgtcg cccggggcgt tggatattga attaaaatta aatcatgaat tgtcaccacg
     3121 aattttttca aattcagcaa cggaacatta aaatgaggaa aatgaaccag atcaacccgg
     3181 tgtttccaaa gcaaataagg catttttagc tgttccgcca gcccgtacca tttaaaatca
     3241 gccagaactt tgctaaaatt gccgttttga ggctgatatt gatcccaatt atcacgccct
     3301 aaaaaaatca gataatgatt ctgatgatca attttttcca aattttctgt cagccgctct
     3361 atatagcggc ccagaccctt ggtctgggga ccgtagaagc gggcgtcaat ggcgattcgc
     3421 attttcttca aataattttt taatttcagc ggccgttttt tgccagctaa actgtttaac
     3481 cttctccaac cctcgttcaa tcaaatcttg tcttaattcc tgatcagtcg ttaaataatt
     3541 catcaaataa gccagttcaa aagagttata ggggtcagcc agcaaacaac tgtcagctaa
     3601 aacttcggct aacgatgaac tatggctggc aataaccggc gtcccggccg ccatggcctc
     3661 cagcggcgga aagccaaagc cttcataaaa agagggataa ataaaaacat cagccagttg
     3721 gtataagccg gctttatctt cagcgtcaac atagcccggc agtttaatat actctttatt
     3781 gggactggca ttgatcgctt ctaaaatatc attcatcagc cagccgccgg agccggccag
     3841 aaccaaataa gtctcaaggg cggttgtcca atatttgctt ttgtaaatat caaaagattt
     3901 tatcaggcca gtaatgtttt ttctgggttc cagagtcccc agatatagaa aaaatttatc
     3961 cggcaaatga tgtttgtttt taacggcggt taattcagct gaagacagct cttttcgcgg
     4021 tcgcgagcca ggataaatga cttcaatttt tgactgatca gtttgataaa gcgccgtcaa
     4081 atcattttta gtgttgaggg aaacggagat aattttatca gccgagttga ttaaactcct
     4141 gggattaacc agccggtgcc aaagcctttt tttaaaatta aaaaatttcg gatatctttc
     4201 aaaacttaaa tcatgaacag tgattacttt cgcgacatca tcgcttaagg aaacgaaatt
     4261 caggttaggc aaaaaaaaca aatcaagctt ttccagttta aactttttgg cgacaagctt
     4321 atcaagtttg ggatatttaa aaaattttaa gctgaaattt aaaagcttat ttggaatccg
     4381 cgaataacag accgcgacat tttcaaagct aaaatcaggc aaagttttct ggtgataagc
     4441 gttggcaaaa aggtaatatt ggttgccttg atcaattcga aataaattat tcagcaaatt
     4501 ataagaatat tcacttacac ctgaatattt tttttcagcc aggcttctga tatcaatgcc
     4561 tattttcata ataaaagact tttatcaata tagtctttta tttttgtttt gaaactgtca
     4621 acattgtatt ttaaagcgtg gcttctgatt tgctcgggat tatatctatt tttatcaaaa
     4681 cggatcacca aatcagccag ttcttcccag gtttgctcct taaaaaactc accggtctgg
     4741 ccggcgatga ctgtttccag ggcgccgccg gcggcgtaag cgatcaccgg ccggcccgaa
     4801 gccattgact caatcggcgt gatgccaaaa tcttcttcct ggggatggat aaaggccagt
     4861 gcccggccgt ataattcagc tttttcggcg tcagtaatct gtcccagaaa ttcaatatta
     4921 tctttggcga ttttttttaa atcttccatg gccggaccga tgccgaatat tttcagactg
     4981 acgcccagcc ggctaaaagc ctgaacagtg atgtcaaacc gcttataaga gaccagccgg
     5041 ccgccggtca gaaaataatc gcctgtcgcc ggcgcgatat taaaaatatc agtttccacc
     5101 ggcggattga taatgtcgct ctgacgccgg taatattttt tgatcctctg ctgaacagtg
     5161 gctgaattcg ctataaacct gtcaacccgg tcagccgcct gtctgtccca aagacgcaat
     5221 ctggttaata gcagagggac tatttttttg accaaccggt tatatttcag ctcctgaaca
     5281 tattcatggg tatcgctcca taaaaacctg gttggcgtgt ggcagtagca aatgtgaagg
     5341 gtttgggggc cggtcagcac tccgttggaa aaggccgagc tgctggataa aaccaaatca
     5401 tagcctttca aattatattt ttcggttgcc aggggcatta aaggcaaaaa ccactggtac
     5461 tttttgacgc ccagcggcat tttttggata aatgaagttc tgatgtcttt ttttaaaaaa
     5521 attggattag ctttgtcttt attatggacc aaaacaaaag tcggcgcccc gggaaatatt
     5581 tcctgaaaca cttttaaaac cttctccgcg cccccgtcct gggcgagata atcgtggacc
     5641 agtgcgactt tcatacaatt tagaaattaa aaatttttag ttatgtgtat tttctttttc
     5701 taagtaatga aaacggcgtt tttaacaaaa tcgcgacatc catcaacaat gaccagctct
     5761 ctatataaaa tgaatccagc ttaacctcgt cttcaaaatc cagatcagac ctgccggaaa
     5821 tctgggccaa tccggtaatt cccggcttga tagtcaaaac aactttgtga tgtttttcat
     5881 accgggccac ttcccgcggc tgatgcggcc gggggccgac cagactcatt tgtcctaata
     5941 a
//
LOCUS       DASWHZ010000183         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_334132, whole genome shotgun sequence.
ACCESSION   DASWHZ010000183 DASWHZ010000000
VERSION     DASWHZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334132"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VGA49_03710"
     CDS             <1..493
                     /locus_tag="VGA49_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664900.1"
                     /translation="FELLVYVAIVSVVIVSAVYFTIDIINASQKSRAFQEVQQNARLA
                     MSRMLQAVQEADDVNTGQSNFDSNPGLLSLAMDDSARNPTIFRVASNALQISQGTGDF
                     IDLTSDKVIVTNLVFTNLSVIDRTKNIKITITVEHINPDNTKAFEASVTLQSAAVIRE
                     RAD"
     gene            617..1027
                     /locus_tag="VGA49_03715"
     CDS             617..1027
                     /locus_tag="VGA49_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664901.1"
                     /translation="MQDYRSKQETGLVALISVIIITVITLGVGVTISLLGLSELQAGL
                     TLDQSSRALETTDSCGEEAYFRLKLDSGYTGGTVPVGTDSCTATVSGGGASRTITTIS
                     TVSDHTRATNVNVDLTTNTDLTAEGIDLTGWGEE"
     gene            1072..1431
                     /locus_tag="VGA49_03720"
     CDS             1072..1431
                     /locus_tag="VGA49_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX9664902.1"
                     /translation="MKLESYRDLIVWQRGVELTTEIYKLTSGFPKSELYGITSQLRRA
                     AVSIPANLAEGYYRNSKKDYQRFCYISFGSGSELETLIIIAKRLKFIPEQAYEKVDLL
                     LEEVMKMMNSLIRKLKQ"
BASE COUNT          487 a          316 c          387 g          463 t
ORIGIN      
        1 atttgaattg ctggtctacg tggccatcgt cagcgtggtg attgtctcgg cggtttattt
       61 tacgattgac attattaacg ccagtcagaa atcaagagct tttcaggaag ttcagcaaaa
      121 cgcccggctg gcgatgagca gaatgcttca ggcggttcag gaggcggatg acgttaatac
      181 cggacagtca aattttgaca gcaacccggg attattgtca ttagccatgg atgattcagc
      241 cagaaatccg accattttta gagtagccag caatgctttg caaatcagtc aaggcaccgg
      301 cgattttatt gatttgacat cagataaagt cattgtgact aatctggttt ttacaaattt
      361 gagcgtgatt gaccggacca aaaacattaa aataaccatt acggttgaac atattaaccc
      421 ggataacacg aaggcatttg aagcgtcagt tactttacaa agcgcagcgg tgattaggga
      481 aagggcagat taaactaata tggggaatca agaggaatca caaggaatca aaagggttta
      541 aacccctttg tttccccttg cttccttctg tttccttatt atattattat aaaatttaat
      601 ctaagaaaat tttaaaatgc aggactatag atcaaaacaa gaaactggcc tagttgccct
      661 gatttctgtt atcataatta cagtgattac tcttggcgtt ggcgtgacga ttagtctgct
      721 tggtttgagc gaattgcagg cggggttgac tttggatcag tccagccggg ctttggaaac
      781 aaccgatagt tgcggtgaag aagcttattt tagactgaaa ctggattctg gctacaccgg
      841 cgggacggtg ccggttggga cggattcttg tacggccacg gtcagcggcg gcggcgccag
      901 ccggacgatc accacgattt ccactgtcag cgatcatacc cgggcgacca atgtaaacgt
      961 ggatttgacg actaatacgg atttaacggc ggaggggatt gatctgacgg ggtgggggga
     1021 ggagtaggga ataggggata ggttataggg gatagtgaaa ggaaaagttc tatgaaatta
     1081 gaatcatatc gtgatcttat cgtttggcag agaggcgttg agttgaccac tgaaatttat
     1141 aagttaacca gcggatttcc caaaagcgaa ttatacggca taacgtcaca gcttcgaagg
     1201 gctgctgtgt caattccggc taatctggcc gagggctatt accggaactc aaaaaaggat
     1261 tatcagcgtt tttgttacat ttcatttgga tcaggatcag aactggaaac tttaatcatt
     1321 attgctaaaa ggttaaaatt tatacctgaa caagcttatg aaaaagttga tcttctgtta
     1381 gaggaagtaa tgaagatgat gaattcatta attaggaagc tgaagcaata aatatttgta
     1441 tcactattaa tctattccct ataccctatt ccctataccc tatcccctat accctatccc
     1501 ctatacccta tcccctaaca acatgtttga ctttctcaaa aagaaaaaat gcttcggcct
     1561 ggatatttcc gatcactcca ttgaagcggt tttgttgtct gaaaaaaaag atcagattac
     1621 tttaaccggc tggagccggg taattttaaa atc
//
LOCUS       DASWHZ010000184         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3306079, whole genome shotgun sequence.
ACCESSION   DASWHZ010000184 DASWHZ010000000
VERSION     DASWHZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306079"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1003)
                     /locus_tag="VGA49_03725"
     CDS             complement(146..1003)
                     /locus_tag="VGA49_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9664903.1"
                     /translation="MKNFTKLLSGLVLILGSLLTFTFFSAPSDQNQLQIIFFDVGQGD
                     SILIKTPYQQKILIDGGPDTTVIEKLGKYLPFYDKEIDLMILTHPHADHVTGLVEVLE
                     RYQVNKILTSGAVHTSAAYLSWLSEIKNQNIPVEIADGYRLIELGEDLKLEILYPLLD
                     FSGQAVDNLNNVSVVAKLVYKNNSFLLTGDAELEAEEALLASGLDLKADVLKVGHHGS
                     STSTSQLFLETVEPDIAVISAGRDNDYGHPSFRTIRRLDRFDAIVYRTDLDGDIELTS
                     DGNNLNVNK"
BASE COUNT          561 a          429 c          388 g          518 t
ORIGIN      
        1 tggcgattca actgtttttg agtcatagta aattctagtg tcatacttga aatggttaat
       61 gattaaccat ttcattatat attaggtatg acattttaat ttccttgtac tatgacatta
      121 tcacttccgt gcgacagtgt ggatattatt tattaacatt caaattatta ccatcgctgg
      181 tcagctcaat atcaccgtca aggtcagtcc ggtaaacaat ggcatcaaac cgatcaagcc
      241 ggcgaatagt tctgaaactg ggatggccgt aatcattatc ccggccggct gaaataaccg
      301 ctatatcagg ttccactgtt tctaaaaaaa gctggctggt ggaagtgctg ctgccatgat
      361 ggccgacttt taaaacatca gcttttaaat caaggccgct ggccagcagg gcttcttcag
      421 cttctaattc agcgtcgccc gttagtaaaa atgaattatt tttgtaaacc agcttagcga
      481 cgactgaaac attgtttaaa ttatcaactg cctgaccgga aaaatccagg agcggatata
      541 aaatttccag ctttaaatct tctcccagct caatcagccg atagccgtca gcaatttcca
      601 ccgggatatt ttgattttta atttctgaca gccagcttaa ataagcggcg ctggtatgga
      661 cagctcctga cgttaaaatt ttattaacct ggtatctttc taaaacttcg accaaaccag
      721 tgacatgatc agcgtgagga tgagtcaaaa tcattaagtc aatttcttta tcataaaacg
      781 gcaaatattt tcccagcttt tcaataaccg tcgtgtccgg cccgccgtca attaaaattt
      841 tctgctgata gggggttttg attaaaatac tgtccccctg gccaacatca aaaaaaatga
      901 tttgaagctg gttttggtcg gacggagcgg aaaaaaaagt aaaagtcagc aaagaaccca
      961 ggattaaaac cagaccggac aaaagtttag tgaagttttt cataaaaata cccccaatta
     1021 atttggtcgg gggtattgat atgatagcaa tttaaaaaat ttttagcaaa aaaaataggg
     1081 tgaaacaggc gcagcttcgt cctttttaag aacaatccga tgttcgtctc acccaatgtg
     1141 ccaagcaact tgcctggttg gataaccagg ctgcctgatg gattgtgagg ggatccgcct
     1201 tcacctaagg ctacggcgga caagcacctc aaatagattg tgaggggaca ttgctagcgt
     1261 catgtcacaa agatacagga ggaatatcgt cccctctcca caaccaacaa agaaagaaag
     1321 taaaaactaa aaagtaaaaa gtcaaaagtt tggtgtccct ccttccactc gggacgattt
     1381 ttatatgttt tggctttgaa ctttgtccgc cggaggcgga tccacggcat gccgccatgg
     1441 cgtgcagcct ttggctgaaa ctttgtactt tgtccgccag aggcgtatcc acggcatgcc
     1501 gccatggcgt gcagcctttg gctgaaactt tgaacttttc ccactttgaa ctttgccagt
     1561 tctggagaga gggtgagaca agagactgct cttcctaaca aggacacttt ttcgctcgtc
     1621 tcacccaatt taccaggcaa cttcctgatt atttagtgag aggacagctg ccgactcgta
     1681 ccacaaagat acaacgcaca tatcgtcccc tctccacaga attcgtatag cttaaatctt
     1741 atccaccaaa ggtggatcag cctccggctg acaacttaca actgctaact aattctctgg
     1801 agagagggta agacaacgcc attatcgcct tataaaagac aaaaatcaga tcgtctcacc
     1861 caatttacca ggcaacttgc ccggttggat aaccgg
//
LOCUS       DASWHZ010000185         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_2947254, whole genome shotgun sequence.
ACCESSION   DASWHZ010000185 DASWHZ010000000
VERSION     DASWHZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947254"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VGA49_03730"
     CDS             <1..705
                     /locus_tag="VGA49_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX9664904.1"
                     /translation="SFIKASPFIDDNWTNQELFRKLKFKKAPIHVLAETLWLLDINKS
                     EDELLANMRKTTRNLIRKAIKENVKITRSNQLADLETFLKLHQLTVKRHNFTPYTDQY
                     FKEQVKAFQAEGQVMIFNASHQGKPLASAVIMFYGEMAFYHHGASVMSKIPASHLIQW
                     AAIKEAQKRNCRFYNFWGIYEGRKKNHPFAGISLFKKGFGGRQKQLLPCQDLPLNKKY
                     FINYFIESVRRIKRGF"
     gene            705..1718
                     /locus_tag="VGA49_03735"
     CDS             705..1718
                     /locus_tag="VGA49_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664905.1"
                     /translation="MANKFDKSGYQFKKTYTNPFKKPQKVNFDVLSSRRGQITKIILI
                     GVLLIWGYIAFYSQQFDLRYIKITGTEIINSDKIHNLINQQKSRFRFLILPQNNIFIF
                     DKNKLADNLHKRFGLKALQIKKNILDRYLEVNLSERTAVMALVDNDGYYYMDRYGYLI
                     TEIQREDVNPEFAQVFNHLSTMPVQLGQQILSRKIVESMLAMIQELKSADVEIASFNI
                     IESPQIELQITRPEPEPAVERETADFDPALAAEIEEEELQPEEIIEIKKIANPDYQYK
                     ELTVITRQGWKIYFGNQIFQENYNLSGQLTSLLTVLNEKLGKDYAGLEYIDSRFGSRV
                     YYK"
     gene            complement(1756..>2487)
                     /locus_tag="VGA49_03740"
     CDS             complement(1756..>2487)
                     /locus_tag="VGA49_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9664906.1"
                     /translation="FIHLKRELEIASRSLENKKQDLEKLKIDLESEKAKLTANKESKE
                     LIIAQTQDSEIKFQNLLYQVRFEKNQINQEILQIENEIREKLNLQKLLNPDFVLNPDK
                     LMWPIVNQGITTYFRDPDYPFRGLVGEHSGLDIRTLINGVPSNGLPVRAAASGIVIKT
                     IDNGRYTGNAVYISHGDLMTIYLHLSQINVSVDDFITIGQAIGLSGGMPGTVGAGLST
                     GAHLHFEVRSNGIPVNPCDYLDPSC"
BASE COUNT          781 a          456 c          500 g          750 t
ORIGIN      
        1 tcatttatta aagcgtcgcc ttttattgac gataactgga cgaaccagga actttttaga
       61 aaacttaaat ttaaaaaagc gccaattcat gtcttggctg aaactttgtg gctgctcgat
      121 attaataaat ctgaagatga gctgctggcc aatatgcgta aaacaacccg gaatctgatt
      181 agaaaagcca ttaaagaaaa tgttaaaata accaggagca accagctggc tgatctggaa
      241 acatttttaa agcttcatca gctgacagtt aaacgccata attttactcc ttatacagat
      301 caatatttta aagaacaggt taaagctttt caggcggagg gacaggtaat gatttttaac
      361 gccagccacc agggcaagcc cctggcttcc gcggtaatta tgttttacgg agaaatggct
      421 ttttatcatc acggcgcttc tgtgatgtcc aaaatcccgg cgtctcattt gattcagtgg
      481 gcggcaatta aagaagctca aaaaagaaat tgccgttttt acaatttttg gggtatttat
      541 gagggccgta aaaaaaatca tccatttgcc ggaattagtt tatttaaaaa aggatttggc
      601 ggccgtcaaa aacaacttct gccttgtcag gatttaccgc tcaataaaaa atattttatt
      661 aattatttca ttgagtcagt ccgccgaatt aaaaggggat tttaatggcc aataaatttg
      721 ataaatcagg ctatcagttc aaaaaaacct ataccaaccc ttttaaaaag ccccaaaagg
      781 taaattttga tgttttatca tccaggcgag ggcagataac aaaaataatt ctgattggag
      841 ttttattgat ctggggatat atcgcgtttt attcccaaca gtttgatttg cgatatatta
      901 agataaccgg gacagaaatt attaattcag ataaaattca taatctgatt aatcagcaaa
      961 aatcaagatt caggttttta atattgccgc aaaacaatat ttttatattt gataaaaaca
     1021 aattggctga taatcttcat aagaggtttg gacttaaagc cctccagatt aaaaaaaaca
     1081 tcctggatcg ttatctggag gttaacctta gcgaaagaac ggctgttatg gccctggttg
     1141 ataatgacgg ctattattat atggatcgtt acggctatct gattactgaa attcagcggg
     1201 aagatgtcaa cccggaattt gcccaggtct ttaatcattt gtcaacaatg ccggttcagc
     1261 tgggccagca gattttatcc agaaaaatag ttgagtcaat gctggcaatg atccaggagc
     1321 taaaatcggc cgacgttgag attgcttcgt ttaacattat agaatctccc caaattgagc
     1381 ttcaaataac gcgacctgag ccagaacctg ctgtagagcg ggagaccgct gattttgatc
     1441 cggccttagc ggctgaaatt gaagaagaag agcttcagcc cgaagaaatt atagaaataa
     1501 aaaaaatagc caatcctgac tatcaatata aagagttaac agtgattacc aggcagggct
     1561 ggaaaattta ttttggcaat caaatttttc aggaaaatta taatttgtcg ggtcagctta
     1621 cgtccctttt aacggtttta aatgaaaaat tgggtaaaga ttatgccggc ctggaataca
     1681 ttgattcgcg ttttggcagc cgggtttatt ataaatagtt aattaaggtt cgcataaggt
     1741 atattgtgcg actcattagc aggaaggatc taaataatcg cagggattga ctggaatgcc
     1801 atttgatctg acttcaaaat gaagatgggc tcctgttgac aggccggcgc cgacagtgcc
     1861 cggcataccg cccgataagc caatagcttg gccaatagtt ataaaatcat caacactgac
     1921 attaatctgg cttaaatgaa ggtaaatggt cattaaatcc ccgtgagaaa tataaacagc
     1981 gttgccggta taacgaccgt tgtcaatggt cttaataaca atgccgctgg cggccgctct
     2041 gaccggtaaa ccatttgaag gcaccccgtt aattaaggtt ctaatatcca ggccggaatg
     2101 ctccccgact aagcctctaa acggataatc aggatcacga aaataagtgg taatcccctg
     2161 gttgacgatt ggccacatca gtttatcagg atttaagaca aaatcaggat ttagaagttt
     2221 ttgcaaattt aatttctccc tgatttcatt ttcaatttgt aaaatttcct gattgatttg
     2281 atttttttca aatctgacct gatataagag gttttgaaat ttgatctctg aatcctgggt
     2341 ttgggcaata atcagttctt ttgattcttt attagcggtt aacttggctt tttcgctttc
     2401 caggtcaatt ttcaattttt ctaaatcctg ttttttgttt tccaggctcc tggaggcgat
     2461 ttccagctcc cttttgagat gaatgaa
//
LOCUS       DASWHZ010000186         6454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3650685, whole genome shotgun sequence.
ACCESSION   DASWHZ010000186 DASWHZ010000000
VERSION     DASWHZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3650685"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1166
                     /gene="fba"
                     /locus_tag="VGA49_03745"
     CDS             312..1166
                     /gene="fba"
                     /locus_tag="VGA49_03745"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HEX9664907.1"
                     /translation="MLTTLKSVLIRAKKGRYAVGAFNTSNLEISQAIIEAAEEMKSPV
                     IISTSEKAIDYAGLSEISAIVYELAQKVKVPVVLHCDHGRDLKIIKSCLDTGRYTSVM
                     IDASHFDYKKNLALTKKIVSWAKPKQVSVEAELGAIAGVEDYIKSKESFFTDPEQARD
                     FVKKTGIDALAVSIGTAHGLAEDIKEEKLDLKRLEQIEKLTKIPLVLHGASQGIPVSD
                     LKKAIKLGIAKINIDTDLRLVFSDSLRKTLKNKKIYDPRDILAPSREAVKKKVMQRMR
                     LFGSINKY"
     gene            1324..2286
                     /locus_tag="VGA49_03750"
     CDS             1324..2286
                     /locus_tag="VGA49_03750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004404796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9664908.1"
                     /translation="MPKIFVTRAIPDAGIKMLRAKKYQVKISPYDRVLKREEIIRMAK
                     GADALLCLLTDKIDSQLLDILGPKLKIVANYAVGFDNIDVKACQKHNVYATNTSGVLT
                     RAVAEHTFALMMAIARRISEADRFVRAGQYVGWDPFLMMGSDVEGKTLGIIGLGRIGR
                     AVAQRAVKGLGMKVLYFDVSRQKDFEKQFKAKFATVNKILKTADFVTIHVPLLPGTKH
                     LIGARELRLMKKTAYLVNTSRGPVIDEKALAKALRQKEIAGAGIDVFEFEPRPVAGLK
                     KLDNIIMTPHIASATIEARQEMSRVAAKNIIFALSGGKPPNLVY"
     gene            2406..2726
                     /locus_tag="VGA49_03755"
     CDS             2406..2726
                     /locus_tag="VGA49_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9664909.1"
                     /translation="MAKTVDQEFVEYIVKSIVSHPDDVQTDRTVDEMGVLITLKINPE
                     DMGYVIGRQGQTARAIRTLLKIVGAKSNARINLKIAEPEGSTRGRRSEADTADVSAVD
                     DLTI"
     gene            2799..3494
                     /gene="trmD"
                     /locus_tag="VGA49_03760"
     CDS             2799..3494
                     /gene="trmD"
                     /locus_tag="VGA49_03760"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9664910.1"
                     /translation="MQFDIITLFPKIFDSYLNETILKRAQGKGLVSIKVHNLRDYSAD
                     KHKTVDDKPFGGGPGMILKIEPIYRCLQKLTGQDLLKRKKLKKKKELIIAFDPAGKTF
                     DQKTAQKFSKARRIILLAGRYEGFDHRVYKIVDQRISIGDYVLSGGELPALVIAEAVS
                     RLIPGVIGHSAETLREETFSFDDQDYIEYPQYTRPEIFEPFRGARWKVPKVLLSGHHQ
                     KIRKWRQSRLKKK"
     gene            3898..5384
                     /locus_tag="VGA49_03765"
     rRNA            3898..5384
                     /locus_tag="VGA49_03765"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            6091..6164
                     /locus_tag="VGA49_03770"
     tRNA            6091..6164
                     /locus_tag="VGA49_03770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6125..6127,aa:Ile,seq:gat)
     gene            6291..6362
                     /locus_tag="VGA49_03775"
     tRNA            6291..6362
                     /locus_tag="VGA49_03775"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6323..6325,aa:Phe,seq:gaa)
     gene            6375..6447
                     /locus_tag="VGA49_03780"
     tRNA            6375..6447
                     /locus_tag="VGA49_03780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6408..6410,aa:Pro,seq:tgg)
BASE COUNT         1867 a         1334 c         1597 g         1656 t
ORIGIN      
        1 caaagggaaa agccctgctg aagctctacg ctggggaact tttaacgccg cttcagttat
       61 tcagcaaatc ggtccgcagg ctggcttact gaaaatcgcg gaaatgaaca aatttttaaa
      121 aagccattct aagatacacg ccaaagaaat ataaaaaaag gcaataggca caaggcaata
      181 ggcacaaggc gaggcaacag ggattaggcg ttagttttta gaaaaaaagt cgcgaggcca
      241 ggcaaccgcc tggcgaagcg acaccaaccc ttgtgccttg tgcctcgtgc cttgtgccta
      301 aaataacaaa aatgttaacc actttaaaat cagtacttat ccgggctaag aaaggccgat
      361 acgccgtcgg cgcttttaat acctccaacc tggaaatttc ccaggcgatt attgaggcgg
      421 ctgaagaaat gaaatcgccg gtgattattt ccacttctga aaaggcgatt gattacgccg
      481 gtttgtcaga aatttcagcc attgtttatg agctggccca aaaagttaaa gtgccggttg
      541 ttcttcattg cgatcacggc cgggatttaa aaattattaa aagttgcctt gataccggtc
      601 gctacacttc agtgatgatt gacgcttctc attttgatta caaaaaaaat ttagctttga
      661 ctaaaaaaat tgtttcctgg gctaagccta agcaagtatc tgttgaagcc gagctgggcg
      721 ccatcgccgg cgttgaggat tatatcaaat caaaagaaag ttttttcact gatccggagc
      781 aggcccggga tttcgttaaa aaaaccggga ttgacgcttt ggcggtttct atcggtacgg
      841 ctcatggtct ggccgaagat attaaggaag aaaaacttga tttgaaaagg ctggagcaaa
      901 ttgaaaaact gactaaaatt cctctggtcc tgcatggcgc ttcgcaggga attcccgtca
      961 gtgatttgaa aaaagccatt aaactgggta ttgccaagat taacattgat actgacttac
     1021 gtctggtctt tagcgacagt ttaagaaaga ctttaaaaaa taaaaaaatc tacgaccccc
     1081 gggacatttt agctccgtca agagaggccg tcaaaaagaa agtgatgcag aggatgagat
     1141 tatttggaag tattaataaa tattaaaaag taaagttcaa agtacaaagt ttcagccaaa
     1201 ggctgcacgc catggcggca tgccgtggat ccgcctccgg cggacaaagt ttcagccaaa
     1261 ggcagatgag cttatggctg aaaaactttt tagttttgaa ttatgatatt taatttttaa
     1321 tatatgccca aaatttttgt cacccgcgct atccccgacg ccgggattaa aatgctaaga
     1381 gccaaaaaat accaggttaa gattagtccg tatgaccggg tgttaaagag agaagaaatt
     1441 atcaggatgg ccaaaggggc tgacgctttg ctttgtttac tgactgataa aattgacagc
     1501 cagcttttgg acatcctggg tcccaaacta aaaatcgtcg ccaactacgc cgttggcttt
     1561 gacaatattg acgttaaggc ttgtcagaaa cataacgtct atgccactaa cacctcggga
     1621 gtgttaacca gggccgttgc cgaacacacc tttgccttaa tgatggccat tgcccggcga
     1681 atttccgaag ccgatcggtt tgtccgggcg ggtcagtacg ttggctggga cccttttttg
     1741 atgatgggtt ctgatgttga aggcaagact ttgggaatta ttggtctggg ccggattggc
     1801 cgggcggttg ctcaaagggc ggttaaaggc ctgggaatga aagttttata ttttgatgtc
     1861 agtcgccaaa aagattttga aaaacaattt aaagctaaat tcgccacagt taataaaatt
     1921 ttgaaaacag ccgatttcgt aacaattcat gttcctttgt tacccggcac taaacatttg
     1981 atcggagcca gagaattaag actgatgaaa aagacagctt atctggtaaa tacttcccgc
     2041 ggcccggtga ttgatgaaaa agctttggcc aaagctttac gccagaaaga aatcgccgga
     2101 gccggcattg atgtttttga gtttgaaccc aggccggtgg ctggtctgaa aaaacttgat
     2161 aatattataa tgacgccgca tattgcttca gcgacgattg aagcccgtca ggagatgtcc
     2221 cgggtagccg ctaaaaatat aatttttgcc ttgagtggcg ggaagccgcc taatttagtt
     2281 tactaaaata tattatccct ttaaccccct ttttcttgac ttttcggtca aactatgcta
     2341 agataatctc ataaaaggtc ggaaattatt tatgtaatta aaagttacta aggttgaaat
     2401 ttaatatggc gaaaacagtc gatcaagaat tcgttgaata cattgttaaa tcaattgtat
     2461 ctcatccgga cgatgtccag acagaccgaa cagttgacga aatgggagtc ctgattactt
     2521 tgaaaattaa cccggaagat atgggttatg taatcggacg ccagggtcag actgcccggg
     2581 ctatcagaac tttgctgaag attgtcgggg ctaaaagcaa cgcccggatc aatctgaaaa
     2641 ttgctgaacc ggaaggttca acaagaggcc ggcggagtga agctgatacc gctgacgtca
     2701 gcgccgtcga tgatttaacg atctaactca catctagttg aaacacagaa gtcgtggtca
     2761 gttttcaacc tgccgcgatt tttgttttta atctaattat gcaattcgat ataattacgt
     2821 tattccccaa gatttttgac agctacttaa atgagacgat tttaaaaagg gcccagggca
     2881 aagggttggt tagcattaaa gttcataatc tccgggacta ctccgctgat aaacataaaa
     2941 ccgttgatga caagcctttt ggcggcgggc cgggaatgat tttaaaaata gagccaatct
     3001 atcgctgttt acaaaaactg accggacaag atttgctgaa aagaaaaaag ttgaaaaaga
     3061 aaaaagaact gatcattgcc tttgatccgg ccggcaaaac ttttgaccaa aagacggctc
     3121 aaaaattttc caaagcccgg agaattattt tactggccgg acggtatgaa gggtttgatc
     3181 accgggttta caaaattgtt gatcagcgga tttcaattgg cgattatgtt ttatccggcg
     3241 gcgaactgcc ggctttggtg atcgctgaag cggtcagccg gctgattccg ggagtgattg
     3301 gccattcagc ggagacgctc agggaggaaa ctttttcttt tgatgaccaa gattatattg
     3361 agtatcccca gtatacccgg ccggaaatat ttgaaccgtt caggggggcg cgatggaaag
     3421 ttcccaaagt attgttgtcg ggtcatcatc aaaaaattag aaaatggcga caatccaggc
     3481 tgaagaaaaa gtagataaat tggggataaa atgtttaaat aattattctt gacaataaaa
     3541 tagcatttaa ctataatgga gttactctaa taaatagtat gaaaatatag tgaaataaag
     3601 caaaattttg aaaactggtt ggaaagcaaa gagtgaaatc taataaaatg aataacaatt
     3661 ggatttctct cttttctttt gacccttaac aactaaagat gcgacagcta aaatctgaca
     3721 ggatccacgt taatttcctt aacagtggtt tttgattttt ttactttaat aagagtttga
     3781 tcccccgcct acgctccttc ggagctccgg cggggcaagc tggcttttta gctctgctaa
     3841 gaagaacagc tgatcactct caaacttcag ttaaatttta ttaaaaattt aattgaactt
     3901 aataagagtt tgatcctggc tcaggatgaa cgctggcggc gtgtctaagg catgcaagtc
     3961 gaacgctccg acgtttcgtc ggagagtggc aaacgggtga gtaacacatt ggtaacctgc
     4021 ctcaaagacg ggtataatcc agcgaaagct ggactaatcc ccgatgtgct taagtagctt
     4081 aagttattta agtaaagctt ttgcgctttg agaggggcct atggcttatc agctagttgg
     4141 tgaggtaaaa gctcaccaag gctacgacga gtagcgggtg tgagagcatg atccgcctca
     4201 agggcactga gacactggcc ttactcctac gggaggctgc agtcaagaat cttccccaat
     4261 ggacgaaagt ctgagggagc gacgccgcgt gaccgaagaa gctcttcgga gtgtaaaggt
     4321 cttttctgtg ggatgaacaa atgaccgtac cacaggaata aggggctgct aacctcgtgc
     4381 cagcagcagc ggtaagacga gggccccaag cgttatccgg aattattggg cgtaaagcgt
     4441 ctgcaggcgg atttttaagt tgaacgttaa atctcagggc tcaaccctga aaccgcgttc
     4501 aagactggaa atctagagga cgggagaggc aagtggaatt gccggtgtag tagtaaaatg
     4561 cgttaatatc ggcaggaaca ccaaatgcga aagcatcttg ctggaacgtc cctgacgctc
     4621 agagacgaaa gcgtgggtag cgaatgggat tagatacccc agtagtccac gccgtaaacg
     4681 atggatacta gacattggaa gtatcgaccc tttcagtgtc gtttacaaaa gataactcgt
     4741 taagtatccc gcctggggag tacggccgca aggctaaaac tcaaaggaat tgacggggac
     4801 ccgcacaagc ggtggaacat gtggtttaat tcgacaataa gcgaggaacc ttaccagggc
     4861 tcaactacta gctgcatatc ctgggaaacc aggaagcctt cgagggtgct agataggtgc
     4921 tgcatggttg tcgtcagctc gtgccgtgag gtgtacggtt aagtccggta acgagcgcaa
     4981 cccccgcgct gtgttaagtt ttcacagcga actgcctacc ttaagtagga ggaaggcggg
     5041 gatgacgtca aatcagcatg gctcttacgt cctgggcgac acacgtgtta caatggccgg
     5101 tacaatgggc tgccaaaccg taaggtggag ctaatcccat caaaaccggt ctcagttcgg
     5161 attgaggtct gcaattcgac ctcatgaagc tggaatcgct agtaatcgca gatcagccac
     5221 gctgcggtga atacgttctc gggtcttgta cacaccgccc gtcacgtcaa gagagtcggt
     5281 aatgcccaaa ccccgacgtc cgtcggggga aggtaggacc gatgataggg acgaagtcgt
     5341 aacaaggcac gcgtagcgga agctgtgcgt ggatcacctc ctttctaggg agataatcgg
     5401 tgtttcacca ggccataacc tggtgagcaa caagacaggt cgggtcctgt tccgtcgagt
     5461 gacgggacgg gacgggaaat taaccctgaa tgatcctgtc agattttagc tgttattttt
     5521 tgcccagaat accctgcggc aagccgcagg gatgaatggc cgcaaaaaat aaatcccgcc
     5581 aacggcggga tcaacaaccg cataccgtcc cgccttggcg ggacggtgcg gttggtgatt
     5641 ataataaaag gtctgtcaca aacagtcttc acggagcaga ctacaccctc aatatatttt
     5701 tcagggttgt agcccgtaga ctgtttttgg cggatctttt tatattaggc acaaggcaca
     5761 aggcaacagg cacaagggtt ggtgtccccg ccttgggcgg gggacaatta tttgatggac
     5821 atcaggcaaa atggttttga ctaatgccta atccccattg cctcgcctgt tgccttgtgc
     5881 ctcgtgcctt gtgcctgctt ccagccttgc taaagagacg attttttgct atgattgatt
     5941 taattagttg gattttttta ggtaaatcag atggtgggcg catatctcag tttccgccaa
     6001 aggcggaccc gcctccggcg ggggttagag ttccgaaaaa taaaaaattt gatttttaaa
     6061 atatcaacta tcaaatttag cgttccaaat gggcgtatag ctcagttggt tagagcgcgt
     6121 cactgataat gacgaggtcc caagttcgaa tcttggtacg cccatttggg acgttatagg
     6181 aaattgaagt taaacaaaat tttttatttt tcgagaatcc ccgatcccgc caaaggcggg
     6241 atcgggacac atgactgata attttctgtt attatgataa ttaatattaa gccgaggtag
     6301 ctcagtggta gagcacatga ctgaaaatca tggtgtcggg agtccgattc tccccctcgg
     6361 catctctata gttacgggga ttggcgcagt tggtagcgcg cttggtttgg gaccaagagg
     6421 tcgcgagttc gagtctcgca tccccgattg agga
//
LOCUS       DASWHZ010000187         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3668201, whole genome shotgun sequence.
ACCESSION   DASWHZ010000187 DASWHZ010000000
VERSION     DASWHZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668201"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VGA49_03785"
     CDS             <1..1062
                     /locus_tag="VGA49_03785"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9664911.1"
                     /translation="EMKTKPTQYATRTLNSVGIQPDFIICRAEKSLDEPRKRKIATFC
                     NIDSDNVISAPDVDSIYEIPVNFEEENLGNKILKKFNLRPRKKDLKEWRKLLKTINNS
                     SKAVKIGIVGKYFGTGDYILSDSYISVIEAVKHASWHYKLVPEIDWLNAEAYQRDPKK
                     LRELKKYDGIIIPGGFGKRGIKGKIKAIEYVRKNKIPFLGLCYGMQLATVEFARNVAG
                     LKNAHTTEIKANTKYPVIDFMSEQRELVKGKKYGGTMRLGAYPCILTKGSRSLKAYGT
                     NVISERHRHRYEFNNDFREALTKKGLALAGLSPDGKLVEIIELKDHPFFVATQFHPEF
                     KSRPLNPHPLFKSFIKACL"
     gene            complement(1124..1435)
                     /gene="rpmA"
                     /locus_tag="VGA49_03790"
     CDS             complement(1124..1435)
                     /gene="rpmA"
                     /locus_tag="VGA49_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX9664912.1"
                     /translation="MAHKKAGGSTSLGRDSQSKRLGVKLFDGQFARAGAILVRQRGSK
                     VRPGKNVGQGVDDTLFALKPGLVKFTRKKLRRFNGQLKLAKFVNILPKGSQQSTVNSK
                     Q"
BASE COUNT          491 a          305 c          330 g          463 t
ORIGIN      
        1 gaaatgaaaa ccaagccgac ccagtacgcc actcggactc taaattcagt tggtattcag
       61 cctgatttta ttatttgccg ggcggaaaag tctttagatg aacccaggaa aagaaaaata
      121 gccacttttt gtaatattga cagtgataac gtcatctccg ctccggatgt tgattcaatt
      181 tacgaaatac cggttaattt tgaagaagaa aatctgggaa ataaaatttt aaagaaattt
      241 aacctgcgtc cccgcaagaa agacttaaaa gaatggcgaa aacttttaaa aacaattaat
      301 aattcgtcca aagctgtcaa aattggcatt gtcggcaaat attttggaac cggcgactat
      361 attttgtctg attcttatat ttcagtgatt gaggcggtta agcacgcctc ctggcattac
      421 aaactggtcc ctgaaattga ctggttgaac gctgaagctt atcagcggga tccaaaaaag
      481 ctaagagaac tgaaaaaata tgatggaatt attattcccg gagggtttgg caagcggggg
      541 attaaaggca agataaaagc aattgagtat gttcgtaaaa ataaaattcc gtttttgggt
      601 ttatgctacg gcatgcagct ggcgactgtt gaatttgccc gaaatgttgc tggtttaaag
      661 aacgctcata cgactgaaat caaagccaac accaaatatc cggtgattga ttttatgtct
      721 gaacagcggg agctggttaa aggaaagaaa tacggcggaa caatgaggct gggagcttat
      781 ccctgtattt tgactaaagg cagccgaagc ctgaaagctt acggcaccaa tgtcatttcc
      841 gaaaggcacc gccaccgtta tgagtttaat aacgatttta gagaggcgct aactaaaaaa
      901 ggtctggcgc tggctggttt gtcgccggac ggcaagctgg ttgaaataat tgagctgaaa
      961 gatcatcctt tttttgtcgc cacccagttt catcccgagt ttaagtctcg tccgttaaat
     1021 ccccatcctc tgtttaaatc atttattaag gcctgtctct agaaattagt ttcaaaacaa
     1081 aaaacccggc ctaaaaccgg attttgtttt gataagttta ttattattgt ttgctgttga
     1141 ctgttgactg ttgacttcct tttggcaata tatttacaaa cttagccagt ttgagctgac
     1201 cgttaaatcg gcgtagtttt ttccgggtaa atttgacaag accaggcttt agagcgaata
     1261 aagtatcatc gactccctgt ccgacatttt tgccaggccg caccttactg ccgcgttgtc
     1321 tgaccagaat cgcgccagcc cgggcaaatt ggccgtcaaa aagtttgacg ccaagtcttt
     1381 tagattgtga gtcgcggcct aacgaagtcg aaccgccagc tttcttatga gccatatatt
     1441 taaaaaaagt ataaaggaga attaaaaaat tgtcaaatca atattatcat tgcatttttt
     1501 ctagttttaa tataaaatta gtctaattat ttccaacaga ttatgcctac ggttattagg
     1561 ataattacca agccattcaa aaaatttct
//
LOCUS       DASWHZ010000188         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_366165, whole genome shotgun sequence.
ACCESSION   DASWHZ010000188 DASWHZ010000000
VERSION     DASWHZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366165"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGA49_03795"
     CDS             complement(<1..188)
                     /locus_tag="VGA49_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9664913.1"
                     /translation="MYYVYVLLSKKDNKLYIGFTNNVKLRFQAHSRGEVASTKNRQPL
                     ELIFYEMFSNKKDALRRE"
     gene            complement(346..736)
                     /gene="ssrA"
                     /locus_tag="VGA49_03800"
     tmRNA           complement(346..736)
                     /gene="ssrA"
                     /locus_tag="VGA49_03800"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(958..1401)
                     /gene="smpB"
                     /locus_tag="VGA49_03805"
     CDS             complement(958..1401)
                     /gene="smpB"
                     /locus_tag="VGA49_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015228901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9664914.1"
                     /translation="MPTVAVNKRANFDYTILETYEAGLVLTGQEVKSIKAGQLSLKGS
                     YVVIKKNEAWLINSHLPPYKMAGKPEGYDPDRNRKLLLHKKEIKTLIGKSQEQGLTLV
                     PLKVYTKRNQIKLEFAVARGKKKIDKRELIKKREIQKKIKTIRKN"
     gene            1497..3233
                     /gene="argS"
                     /locus_tag="VGA49_03810"
     CDS             1497..3233
                     /gene="argS"
                     /locus_tag="VGA49_03810"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9664915.1"
                     /translation="MKEQIKDLINQALDQAKKDTRLPDFVLGEEIQIEHPQLTEHGDY
                     ATNIALKLAKIVKLKPVAVAELVVEEILKLKEVKKNFSSVRVNRPGFINFIFSKDYLV
                     DQANQVKKLKDKFGGGSLGAGQTVVIDYSSPNIAKPMHVGHLRSTVIGDSLYRLAKFL
                     DYKTISDNHIGDWGTQFGLLLYAYKNFGDKKKVEKDPINELNKLYIDASQQAKDDKKI
                     KAAGKAEFKKLEDGDPENKELWLWFTEVSMKKFETAYQLLGINKFDYVLGESFYEDKM
                     SREFDELLEKGIAKKDPDHSIYVDLEPYKLGRCILVKSDGATTYHLRDIATYRYRLEE
                     FKFDQNLYVVDFRQSHHFLQLFKVLELMGLPEVDQSFHVSFGMLKLPSGSTMSTREGN
                     LVELELLIKEGVEKAQKVISEKNPNLKNKDEVARQIAVGAIKYADLSHNRETDIIFDW
                     DKYLQFEGNTGPYLQYTHARIKSILSKTDFKFSKINPDHLTGKEEIALLRKLYIFPEI
                     VQQAFEDYLPSLIANYLFQLASDFNLFYNNLPVLKADDKIKKARLNLAAAVAQILKNG
                     LDLLGIEAPEEM"
     gene            3306..4325
                     /locus_tag="VGA49_03815"
     CDS             3306..4325
                     /locus_tag="VGA49_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9664916.1"
                     /translation="MIDSSSNKSQLVNLSTWTILKVILILFLLWFVYVVRDILAVLFV
                     AILLTSLLEPFVTWLQQKMKFPRSISVILIYVVVIGLVSLSLVLIIPPIVHQLTNLIK
                     VFPAFFSEHNFISDYFGRAEIFEGLKTGLSSLESSLGRTATGVYSTVSSIFGGIISLI
                     LVLVITFYMTIEEDATRRVLKSVTPDKYLPYVTQLTNRIQNKLGLWLKGQLILSLILG
                     IISYIGLKILGIEYALILAVLAGLAEFVPFLGPIIAGIPAVILAFFQEPIKAVFVIIL
                     YIIIQQVENHILVPKVMQKTVGLNPIIVIVALLVGAKLAGVLGALLAVPVATAIGVFI
                     KDFYS"
     gene            4334..>5062
                     /locus_tag="VGA49_03820"
     CDS             4334..>5062
                     /locus_tag="VGA49_03820"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9664917.1"
                     /translation="MNEKLMDKLVSLAKRRGFIYPSSEIYGGFANSYNYGPYGVELKN
                     NIKILWWRMFVHQRPDIVGLDGSIILHPKVWEASGHTTGFNDALVDCKSCKNRFRADH
                     LIEAALDQDMEGKPLAAMDKLIKDKKIKCPVCKKSDWTKIRQFNGMFKTHFGTQETED
                     NLAYLRPETAQAIFVEYKNIIDTMRVKIPFGIAQIGKAFRNEITPGNFIFRTREFEQM
                     EIEYFIREKDWKKVFEKWQTAMEKW"
BASE COUNT         1453 a          928 c         1081 g         1600 t
ORIGIN      
        1 ttttctcttc ttaaggcgtc ttttttattt gaaaacattt cataaaaaat taattcaaga
       61 ggttggcgat tcttagttga tgctacctct ccccgagagt gggcttgaaa tcttaattta
      121 acattgtttg taaagccgat gtataattta ttgtcctttt tacttagaag aacgtagaca
      181 taatacataa atttttttag agccccgaac cactcataac ccggctgctg ccgggttctt
      241 cgggttcagg gccatgaacc attcgccaga atttaaatgg ttgtattcat gacagaacct
      301 aatggcctgc catgaacctg agctgggctt tgcccagcga atggttcatg gagatggcgg
      361 gaatcgaacc cgcgtctaga atttcataat aaaatagtct acaagcttag tttgatttac
      421 aatttagatt ataaaactaa aaatcagaca aaatatttta taaccgagcc gttaatctca
      481 attattcttt tggcggaaga ataatccagt cccataatta tgacaccgga tactttctat
      541 ggaacctcaa aagtccgatg ggccgctaag ccttattagg caacggccat tgccaggcta
      601 ggaactctga aaagagactt agccttggcg agtaagttgt ttgcacttac aattttgctg
      661 ataattttta cgggttaacc agcctgcccg gcttgcctgt tttaatataa aaaaactatc
      721 gagaccattc atcccctatt ggttattcag ctcgcttgcc ccgcgcctga accggcaaag
      781 ccggtgaatg gcggcggggt cgagaccatt catccccact aaaacagtca acagccaacc
      841 ccgtcgctcg cgagcgacgg ggcaacagcc aactgtcatc agagtatgga agcaaaccct
      901 gttgactgct gactacttcc gccggaggcg gatccgcctt tggcggagac tgctgactta
      961 atttttcctg atcgtcttta tttttttctg aatttctcgt tttttaatta attcccgttt
     1021 atcaatcttc tttttacctc tggctacggc aaattccagc ttaatctgat ttctcttagt
     1081 atacactttt aagggcacta atgtcaagcc ctgctcctgt gatttgccaa ttaaagtttt
     1141 aatttccttt ttgtgcaaaa gcagcttgcg atttctgtca ggatcataac cctctggctt
     1201 tccggccatt ttatatggcg gcagatggga gttgatcagc caggcttcat tttttttaat
     1261 caccacatac gagcctttaa gactcagctg gccggcttta attgatttga cttcctgacc
     1321 ggtcaaaacc agcccggctt cataggtttc caggatagta taatcaaagt tggctctttt
     1381 gttgacggca actgttggca tataattcta atttattata gctgcaaatg ttgacaaatt
     1441 caaccaaaaa tcagatactt tgtctaattc tttgaaaaaa gttataatat ttataaatga
     1501 aagaacaaat taaagattta attaaccagg cattggacca ggctaaaaaa gataccagac
     1561 tgcctgactt tgtgttgggg gaagaaattc agatagagca tccccagtta accgagcatg
     1621 gtgactacgc taccaatatc gctttgaagc tggctaaaat tgttaaatta aaaccagtcg
     1681 ccgtggcgga gcttgtcgtt gaagagattt taaaattaaa agaagttaag aaaaatttca
     1741 gttcagtccg ggttaaccgg cctggtttta tcaatttcat tttttccaaa gattatctgg
     1801 ttgatcaggc taatcaggtt aaaaaattaa aagataaatt tggcggcggc agtctgggtg
     1861 ctggtcagac ggtagtgatt gattattcat cgcctaatat cgccaaaccg atgcatgttg
     1921 gtcatttgag atcaactgtt attggcgaca gcctttatcg cctggctaaa tttttagact
     1981 ataaaacgat ttctgataat catattggtg attgggggac ccaatttggt ttgttgctct
     2041 acgcttataa aaattttgga gataaaaaaa aggtagaaaa agatccgatt aatgaactga
     2101 acaagcttta tatcgatgcc agccaacaag ccaaagacga taaaaaaatc aaagccgccg
     2161 gcaaggctga atttaaaaaa ctggaagacg gcgatccgga aaataaagaa ctctggctgt
     2221 ggtttactga agtatccatg aaaaagtttg aaacagccta tcagcttttg ggcatcaata
     2281 aatttgatta tgtccttggc gagagttttt atgaagataa aatgagtcgg gagtttgatg
     2341 aattgctgga gaaggggatt gccaaaaaag atcccgatca ctcaatttat gttgatctgg
     2401 agccttataa actcggccgg tgtattttgg ttaaatctga cggcgccacg acttatcatc
     2461 tgcgtgatat tgccacttat cgttatcggc tggaagaatt caagtttgat caaaatttat
     2521 atgtggttga ttttcgacag agccatcatt ttttacagct ttttaaagtc ctggaattaa
     2581 tgggattgcc ggaagttgat cagtcttttc acgttagttt tgggatgtta aaactgccgt
     2641 ctggttcaac tatgtcgacc agggaaggca acctggttga gttggaactt ttaattaaag
     2701 aaggtgttga aaaggctcaa aaagtaattt ctgaaaaaaa tccaaatctt aaaaataaag
     2761 atgaggtggc ccgccagatc gcggtcggcg ccattaaata tgctgattta tcccataacc
     2821 gggaaacaga cattattttt gactgggata agtatcttca gtttgaaggc aataccggcc
     2881 cttacttaca atatacccac gcccgaatta aaagtatttt aagtaaaact gattttaagt
     2941 tttcaaaaat taatccggac cacctgacgg gaaaagaaga aatcgctctt ttaagaaaat
     3001 tatatatttt tccggaaatt gttcagcaag cttttgaaga ttatctgcca agtctgatcg
     3061 ccaattattt atttcagctg gcttcggatt ttaatctttt ttacaataat ctgcctgttt
     3121 taaaagctga tgataaaatt aaaaaggctc gtttgaattt ggcggccgct gtcgctcaaa
     3181 ttttgaaaaa tgggctggat ttattgggta ttgaagcccc ggaagaaatg tgacgagcaa
     3241 gggagatgaa ggtttattaa gggtgataaa gggatatcaa gggtattgga attaaaattt
     3301 ttaaaatgat tgattcaagt tcaaacaaaa gtcagttagt taatctctcc acctggacga
     3361 ttttaaaagt tattttaatt ttgtttttac tgtggtttgt ttatgttgtc cgggatattt
     3421 tggcggtttt atttgtggct attctgctga cttccctgct tgaaccgttt gttacctggc
     3481 ttcagcaaaa aatgaaattt ccccggtcga tcagcgttat tttgatttat gtggtggtca
     3541 tcgggctggt cagcctgtca ttagttttga ttataccgcc aattgttcat cagctgacta
     3601 atttaataaa ggtttttccg gcgttttttt ctgaacataa ttttatatct gattattttg
     3661 gacgggctga aatttttgaa ggcctgaaaa ccggtttgtc atctttggag tcatcactgg
     3721 ggcggacggc cactggtgtc tactcgactg tcagttcaat ttttggaggc attatttctt
     3781 tgattttagt gctggtgatt actttttaca tgacaattga agaagacgcc accagacggg
     3841 ttttaaaatc agttacccct gacaaatatc tgccttatgt cacccagtta accaaccgga
     3901 ttcaaaataa gcttgggtta tggctgaaag gtcaattaat tttaagtttg attttaggta
     3961 taatctctta tatcggtctg aaaattttag ggattgaata tgctttgatc ctggcggttc
     4021 tggccggtct ggctgaattc gtgccttttt tggggccaat cattgccggg atcccggcgg
     4081 tcattttggc cttttttcag gaaccaatta aggctgtctt tgtcatcatt ctctatataa
     4141 ttattcaaca ggttgaaaat catattttgg tgccaaaagt tatgcagaaa acagttgggc
     4201 tgaatccaat tattgtcatt gttgcattgc tggttggagc aaaactggcc ggcgttcttg
     4261 gagctttgct ggccgttccg gtcgccacgg ccatcggtgt ttttataaag gatttttata
     4321 gttaaaataa tttatgaatg agaaattaat ggataaattg gtttcactgg ccaaaagaag
     4381 gggatttatt tatccgtcgt cagaaattta cggcggtttt gccaattcat acaattacgg
     4441 tccatacgga gttgaattga aaaataatat taaaattctt tggtggcgga tgtttgttca
     4501 tcagcggccg gatattgttg gtttggacgg cagtattatt cttcatccca aagtttggga
     4561 agcttccggc cacaccaccg gttttaatga cgctctggtt gactgcaagt cctgtaaaaa
     4621 tcgtttccgg gccgatcatt tgattgaggc cgctttggac caggatatgg agggtaaacc
     4681 cttggccgca atggataagc tgattaagga taaaaaaatt aaatgtcctg tttgcaaaaa
     4741 atctgactgg accaaaatcc gccagtttaa cgggatgttt aaaacgcatt ttggcactca
     4801 ggaaaccgaa gataacctgg cttatttgag gccggaaacc gcccaggcga tttttgtgga
     4861 atataaaaat attattgaca cgatgagagt taaaattcct tttggcatcg cccagatcgg
     4921 taaagctttt agaaatgaga ttactccggg caattttatt ttccgaacca gggaatttga
     4981 acagatggaa attgaatatt tcatcagaga aaaagactgg aaaaaagttt ttgaaaaatg
     5041 gcagacagct atggagaaat gg
//
LOCUS       DASWHZ010000189         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_3669973, whole genome shotgun sequence.
ACCESSION   DASWHZ010000189 DASWHZ010000000
VERSION     DASWHZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3669973"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VGA49_03825"
     CDS             complement(<1..1292)
                     /locus_tag="VGA49_03825"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX9664918.1"
                     /translation="MSKDDSTKKEILTKLKPPKWLIILLAVFLLFSGILAGSVYAYQK
                     AYASRIYPGVMIGQFKVGGQPPQKIAEALQQKAGQINREGLKFQHGNQRVDVLPVLSA
                     ADPDLAKEVFSFNINQTVSRALSVGRQGNFLKKLFGQLSALMFPKRLEIVYRLNQEEL
                     LNILKNSFSQFEQPAESARLKVLADNQLTVISEKSGSVFDYHTVIAEAGNNLSQLTDT
                     AIALNLVKDEPNIKSQDTAEAVEFAKTLITRQPPILKYEDQTWELTEEDLAQNINFVS
                     NSPVITAAFDPKLFGKYLGKIAEEINVEPREAKFVIQSDQRVKEFQASREGIKLNIEE
                     TVKKLNQEFIKNNQTEIELVVETDQPQATTENINDLGIREVIGVGTSNFAGSPPNRRH
                     NIRVGAAALNGLLLKPGEEFSLIKALGNIDAANGYLPEL"
     gene            complement(1401..1649)
                     /locus_tag="VGA49_03830"
     CDS             complement(1401..1649)
                     /locus_tag="VGA49_03830"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HEX9664919.1"
                     /translation="MTDEVLDVSQKKFHLKAVIDRFEGIMAVLKFDDNQEINWPIKNL
                     PEDCQAGSVVRLIISTSKTDQEEREKMAKTLLNEILKK"
     gene            complement(1655..2182)
                     /locus_tag="VGA49_03835"
     CDS             complement(1655..2182)
                     /locus_tag="VGA49_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9664920.1"
                     /translation="MGKQPLLSKILGLKDKGDDKLFVVPKDLDQDSEAEKLEPDTSDN
                     DQLIKSKLEEIQDLDEWFKDNIEGQLAIDVFYDDDNLIIKSAIAGVKAESIDISLKND
                     MLTIRGYRGSGTNIDPENYICQECYWGGFSRSIILPVEVYADRIDAKIKNGILTVTLP
                     VLKKTKPINIKPIEI"
     gene            2468..2542
                     /locus_tag="VGA49_03840"
     tRNA            2468..2542
                     /locus_tag="VGA49_03840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2501..2503,aa:Glu,seq:ttc)
     gene            2549..>3439
                     /locus_tag="VGA49_03845"
     CDS             2549..>3439
                     /locus_tag="VGA49_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEX9664921.1"
                     /translation="MQNSESNILAKAFLIVGISLALGLIFDYFFYIKIPGISFPLYIS
                     LVIIALFLISIFIKKKLQKQILWLILPLMFFSFMVFIRSSVLLTFLNIITSLLILLVI
                     AKIAFKIKLKNFVIKNYLEILLLPFDFLEPLCKTLGNIFKLRGADNNQKILPKIIKGI
                     LITIPVLLIFLLLFASADLIFQRYLSNLTNIFTLSEETFSRIVLIIMVTLAFTGAYSY
                     IFGKPENEETLLNNGQTISLGHIENSILLISVNILFFIFIVIQLTYLFGFENTIASQG
                     FTYAEYARKGFFELIAVAVIS"
BASE COUNT          907 a          746 c          648 g         1138 t
ORIGIN      
        1 agttcgggca gatagccatt agccgcgtca atattgccca aagctttaat cagagaaaac
       61 tcttcgcctg gcttaagtaa caaaccgttt aaagcagcgg cgccaacccg gatattatgg
      121 cggcgattgg gcggcgatcc cgcaaaatta gaagtcccca ctccaatcac ttccctaatt
      181 cccaaatcat taatgttttc cgtggtcgcc tggggctggt cagtctcaac caccagctca
      241 atttctgtct gattattttt aataaattcc tgatttaatt ttttgactgt ttcttcaata
      301 tttaatttaa ttccctcccg gctggcctgg aattctttaa cccgctggtc agactgaatg
      361 acaaatttgg cctcccgggg ctcaacattg atttcttcag cgatttttcc taaatacttt
      421 ccaaacaact tcggatcaaa agcggccgtg ataaccgggc tgttactgac aaaattaata
      481 ttttgagcca ggtcttcttc agtcagctcc caggtttgat cttcatattt taaaattggc
      541 ggctgtctgg taattaatgt tttggcaaac tcaaccgctt cagccgtatc ctggctttta
      601 atattcggtt cgtccttaac cagatttaaa gcaattgccg tgtcagtcag ctggcttaaa
      661 ttattaccag cttcggctat gactgtgtga taatcaaaga cagaaccgga tttttctgaa
      721 atcaccgtca gttgattgtc tgccagcact tttagccggg cgctttcggc cggttgttca
      781 aactggctga agctgttctt taaaatattc aggagttctt cctggttaag gcggtagacg
      841 atttccagtc ttttgggaaa cattaaagct gacaactggc caaataattt tttcaaaaaa
      901 tttccctgcc ggccaacgct taaagcccgg ctgacggtct gattaatatt aaaactaaaa
      961 acttctttgg ccagatcggg atcagcggcc gataaaaccg gcaaaacatc cacccgctgg
     1021 tttccatgct gaaattttaa accttcccgg ttaatctggc cggctttttg ctgtaaagct
     1081 tcagcgattt tttgaggcgg ctgaccgccg actttaaact gcccaatcat caccccggga
     1141 taaatccggc tggcgtaagc tttttgataa gcataaactg aaccggccaa gattcctgaa
     1201 aacaataaaa aaacggctaa caatataatc agccatttgg gtggtttgag cttagttaaa
     1261 atttccttct ttgtggaatc gtctttggac atagagaaaa actaagaacc tgagcaacca
     1321 gggatacaga gattcagcgc tttagcacaa tagcacgctt gtttttcagt tccttagttg
     1381 cttagttctt atgttatttc ttatttcttt aatatctcgt ttaacaatgt cttggccatt
     1441 ttttcccgtt cttcctgatc ggtttttgaa gttgaaataa tcagccggac aaccgaaccc
     1501 gcctgacagt cttccggcag gtttttaatc ggccagttga tttcctgatt gtcatcaaat
     1561 tttaaaacag ccattatgcc ttcaaaccgg tcaatgactg ctttcaaatg aaactttttt
     1621 tggctgacat ccaaaacttc gtcagtcata ggaattaaat ttcaataggt ttaatattga
     1681 ttggcttggt tttctttaaa acaggcaaag ttactgtcaa aattccgttt ttaatcttag
     1741 cgtcaatccg gtcggcgtaa acttccaccg gcaggataat cgaccggctg aagccgcccc
     1801 agtagcattc ctggcaaata tagttttcag gatcaatgtt tgtgccggat ccccggtaac
     1861 cccggatcgt cagcatatca ttttttaaag aaatgtcaat gctttcggcc ttaacgccgg
     1921 caatggctga tttgataata agattatcgt catcataaaa aacatctata gccagctggc
     1981 cctcaatatt gtctttgaac cattcatcca aatcctgaat ttcctcaagt tttgatttga
     2041 tcagctgatc attgtcgctg gtgtccggct ccagcttttc tgcttcagag tcctgatcca
     2101 gatctttggg cacgacaaac aatttgtcat ctcctttatc ttttagtcca agtatttttg
     2161 acagcaaagg ttgtttgccc atattagata attatgccgg ttttgatatt tttttgcaaa
     2221 ccaaaaatct tgacttttgg cggattttag gctagtctgt ataaatggtt ttaaccgcgc
     2281 ccccttcgcc ccgcccttcg cgaagggcgg ggcttcgtct agtccctgta ccactctccg
     2341 gctgttgccg gatcgggtac agggcaaggg ttaagacatc aggtttttcc gccaaaggcc
     2401 tgccggcagg caggcaggcg gacccgcctc cggcgggcat cctggctcat atatgtataa
     2461 tgattatgcc cccttcgtct agtggttagg acatcaggtt ttcatcctga aaacaggggt
     2521 tcgattcccc tagggggtac caatagatat gcaaaattca gaatcaaata ttttagccaa
     2581 agcttttttg atcgttggga tatctctggc tttgggtttg atcttcgatt atttttttta
     2641 tattaaaatt cccgggatat cattcccttt atatattagt ctggttatta tagccctttt
     2701 tttaatttca atttttatta aaaaaaaact acaaaaacaa attttgtggc ttattttgcc
     2761 tttaatgttt ttcagtttca tggtttttat tcgttcaagc gtcttattaa cattcttaaa
     2821 tataataacc agtttgctga ttttattggt tatcgccaaa atagccttta aaatcaaact
     2881 gaaaaatttc gttatcaaaa attatcttga aatcttgctc ctgccatttg attttttaga
     2941 gccgctttgt aagacattgg gaaatatatt taagctgcgc ggtgctgata acaaccaaaa
     3001 aattcttccg aaaataatta aaggtatttt aataaccatt ccagttttgc taattttttt
     3061 attgcttttc gcttcagcag atctaatttt ccaaagatat ctctcgaatt taactaatat
     3121 tttcacatta agtgaagaga ccttctccag aatagttttg attattatgg tgactttggc
     3181 atttacaggt gcctactcct atatattcgg gaaaccagaa aacgaggaaa ctcttctgaa
     3241 taacgggcaa acaatcagcc tgggacatat tgaaaactct attcttttaa tttcagttaa
     3301 tatcctcttt tttattttta tagttattca gctgacgtac ctgtttgggt ttgaaaacac
     3361 tatcgcctct caaggtttta cttacgccga atacgcccgg aaaggttttt ttgaattgat
     3421 tgccgtagcg gtcatttca
//
LOCUS       DASWHZ010000190         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate SMAG_U2855
            k141_366412, whole genome shotgun sequence.
ACCESSION   DASWHZ010000190 DASWHZ010000000
VERSION     DASWHZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960502
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (soil metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:39:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 775
            CDSs (total)                      :: 740
            Genes (coding)                    :: 733
            CDSs (with protein)               :: 733
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366412"
                     /isolate="SMAG_U2855"
                     /isolation_source="soil"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VGA49_03850"
     CDS             <1..657
                     /locus_tag="VGA49_03850"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664922.1"
                     /translation="FEFTRQILQGRSGEIRGDEGKLGENFLDDWKLLGQVRVSYLIVE
                     SRKGLIVIDQHAAAERVLYDKLKNQLKAEQPRSQKLLLPINLELSAVEVDLLKENQDL
                     LGKIGFMIDEFGGNSFNINAVPADISQYEIKKVLLGVISDLEDQEFRKTKIVEEKQDI
                     VIKYTACRSAIKFNDQLTWEEQIQLLKDIKRYNVISCPHGRPCLWEVGWSELEKKFKR
                     "
     gene            complement(902..1237)
                     /locus_tag="VGA49_03855"
     CDS             complement(902..1237)
                     /locus_tag="VGA49_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664923.1"
                     /translation="MPLKKQPANKNLTALLRKAKKEIKKLESHLLRLEADLSFCRQQI
                     EKTQNLLKGKIFEHGLEKKLTFDQLYSLKRDLENLDNKKNETAYQLAFQKTFVRTKKR
                     NLLEYINKG"
     gene            complement(1312..1548)
                     /locus_tag="VGA49_03860"
     CDS             complement(1312..1548)
                     /locus_tag="VGA49_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9664924.1"
                     /translation="MKHWARQIIIGGLGKHSLPDLELQKRGVDTETLIKFRDRLIGIT
                     GGNPLKDNEVNDQELTKLLRQAIEEKLAKQEVSA"
     gene            complement(1862..1933)
                     /locus_tag="VGA49_03865"
     tRNA            complement(1862..1933)
                     /locus_tag="VGA49_03865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1898..1900),aa:Arg,seq:tcg)
     gene            complement(1953..2993)
                     /locus_tag="VGA49_03870"
     CDS             complement(1953..2993)
                     /locus_tag="VGA49_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX9664925.1"
                     /translation="MLIQDIFKAAHQQQGSDVHLVVGYRPIVRIDGRLSELKKFKKIT
                     AKTAREISQGICDKTQQDFFNIDKELDLSYETPDKVRYRINLHLQKGEVGLSARLIPK
                     NIPTMEWVAMPEIVYELIQRKNGLILVTGASSHGKSTSLAAMINEINKTRAEHIITLE
                     DPIEYLFTPVKSVIEQRELGPDMRSFNQALKRTLRQDPNVIMVGEMRDLESIASTVTL
                     AETGHLVFATLHTFSAAQTIDRIIDMFPPGQQKQISGQLSITLAGIITQKLLPKIGGG
                     RVAVKEILVNNNAIANLIRENKINQINSVIQTSAAEGMITMDKALAEAYKKKLITKET
                     AMAHANNPRVIK"
     gene            complement(2999..3688)
                     /gene="rnc"
                     /locus_tag="VGA49_03875"
     CDS             complement(2999..3688)
                     /gene="rnc"
                     /locus_tag="VGA49_03875"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9664926.1"
                     /translation="MKDFSKLQKSLKVKFKNIDLLKQSVVHRSYLNEHPSFKLDHNER
                     LEFLGDAVLELIVTEYLYLNFPNPEGELTNWRASLVNAKMLAEASSALEVEPFLFLSK
                     GEAKDTGKARQFILANAFEAIIGAVYLDRGWEIAQEFINRTIISQLPHILENQLYLDP
                     KSKFQEMSQEKLGITPNYKVIDEKGPDHAKIFTIGVYLDDQLVAKGKGDSKQEAQVEA
                     AAKALKIKNWE"
BASE COUNT         1006 a          773 c          904 g         1143 t
ORIGIN      
        1 tttgagttta cgaggcaaat tttacaaggg agatcagggg agatcagagg ggatgaaggg
       61 aaattagggg agaatttttt ggatgactgg aaattattag gacaagtcag ggtttcttac
      121 ttaattgttg aatctagaaa gggattaatt gttattgatc agcacgcggc ggctgaacgg
      181 gtcttatatg ataaactgaa aaatcagctg aaagctgaac agccgcggtc ccagaaatta
      241 ttattgccaa tcaacctgga gctgtcggct gttgaagttg atttattaaa agaaaaccag
      301 gacctgctgg gaaaaattgg ttttatgatt gacgagtttg gagggaattc ttttaatatt
      361 aacgccgtgc cggctgatat cagccagtat gaaataaaaa aagtcctgct gggggtcatc
      421 agcgacctgg aggatcagga gtttagaaaa actaagattg ttgaagaaaa gcaggatata
      481 gtcattaaat ataccgcctg ccggagcgcc attaagttta atgatcagct gacctgggaa
      541 gagcagattc agcttttaaa agacattaaa agatataatg taatttcgtg tccgcatggg
      601 cggccgtgtt tgtgggaagt cggctggagc gagctggaga agaaatttaa aagatagtgg
      661 cttttgctgg atatttcaaa tttgaaaata tttgctgata taaaactatt ctgccctttt
      721 tgatggcaaa aaggaccaaa aaccactggg gtagccaatt cgcctgtgtg ctattgcgat
      781 aatcagcttt tgctacgggc tcaaggctac cccagacccc ttggtgcaga gagcaatgaa
      841 agcattttga tctttttaaa aggattccct gatttccctt gatgtcccct gataatccct
      901 gctatccctt gtttatatat tccagcaggt ttcttttttt tgttctaaca aatgttttct
      961 gaaaagcgag ttggtaagca gtctcgtttt ttttattgtc cagattttcc agatctcgtt
     1021 tgaggctgta gagctggtca aaggtcagtt ttttttccag accgtgttca aaaattttgc
     1081 cttttaataa attttgggtt ttttcaattt gttggcggca aaatgataaa tcagcctcca
     1141 gccgcaacaa atggctttcc agttttttga tttctttttt ggcttttcga agcaaggcag
     1201 ttaaattttt attcgcgggt tgttttttaa gaggcatatt aagaaattat agcataaaaa
     1261 aacgccctgg taagtctgtg gactaaccag ggcgcggaag tcagacctat cctaggcgct
     1321 gacttcctgc ttggcaagct tctcttcgat ggcttgacgg aggagctttg tcagctcctg
     1381 gtcattgacc tcgttgtctt tgagcgggtt gccgccagta attccgatca atctgtcgcg
     1441 aaacttgatc agagtttctg tgtcgactcc tcgcttctgg agttccaggt cgggcaaact
     1501 gtgcttgccc aaaccaccga ttatgatttg acgagcccag tgtttcatgg ttttggttac
     1561 ttgtgccaag cttgttcccc ccttctagga aacagtgagt atgaacaggt tacaaacaac
     1621 ataaaccccc ctcagcaggt ttatagatta agatataata ttgattagct cttgtcaagg
     1681 ctttttaaaa cagttaatag ccaatcccgc cggcattgct ggcgggacag gccgtcaaca
     1741 gccaaatggg gaggggctat ggtttagtga ttagtctaaa aaaaagacct tgttttaaga
     1801 tctttttaaa ctgcgtccgg gcaaactcct aacctcgtgg tttcccgcct gttttgtcat
     1861 ttgctcccgg agggattcga acccccaata cgacgtccga agcgtcgcgt gatatccatt
     1921 tcaccacggg agcaagtacg gcaggcggtt attcatttaa tcactctggg attattggcg
     1981 tgagccatgg ccgtttcttt ggtgatcaat tttttcttat aagcttcagc cagagcttta
     2041 tccatggtaa tcatcccttc agcggcgctg gtttggataa cgctgttaat ttgattaatt
     2101 ttattttccc ggatcagatt agcgatggcg ttattattga ccaaaatttc tttgacagcc
     2161 acccggccgc cgccgatttt tggcaaaagt ttttgagtaa taattccggc cagagtaatt
     2221 gacagctgac cggagatttg tttttgttgg ccgggaggaa acatgtcaat gatccggtca
     2281 atcgtctggg cggcgctgaa agtgtggagg gtggcgaaaa ccagatggcc ggtttcagcc
     2341 agagtgactg ttgaagcaat tgattccaga tccctcattt cgccgaccat aataacattg
     2401 ggatcctggc gcaatgttct tttcagcgcc tggttgaatg acctcatgtc cggtcccagc
     2461 tcccgttgtt caatgacgct tttgaccggg gtgaataagt attcaatcgg gtcttccagg
     2521 gtaataatat gttccgccct ggttttattg atttcgttga tcatggccgc cagggaggtt
     2581 gatttgccat gactgctcgc cccggttact aaaattaagc catttttcct ctggatcagc
     2641 tcataaacga tttccggcat ggcgacccat tccatggtcg ggatgttttt tggaatcaac
     2701 cgggccgata agccgacttc gcctttttgg aggtgaaggt tgattctgta acggactttg
     2761 tccggggttt catatgacaa atcaagctct ttatctatat taaaaaaatc ctgctgggtt
     2821 ttatcacaaa taccttgtga gatttcccgg gcggttttgg cggtaatttt tttaaatttt
     2881 tttaattcag acagccggcc gtcaattctg acaatcggcc ggtagccaac gaccagatga
     2941 acatcagacc cttgttgttg atgagcggct ttaaaaatat cctgaatgag catagttttt
     3001 attcccagtt tttaattttt aacgccttag cggcggcttc aacctgggcc tcctgtttgc
     3061 tgtcgccttt gcctttggcc accagctggt cgtctaaata aacgccaatt gtaaatattt
     3121 tagcgtgatc cggacccttt tcatcaatga ctttataatt tggggtaatg ccgagtttct
     3181 cctgggacat ttcctgaaat ttgcttttgg gatcaagata aagctggttt tccagaatgt
     3241 gcggcagctg gctaataatg gttctgttaa taaattcctg ggctatctcc cagccccggt
     3301 caagataaac agcgccgatg atcgcctcaa aagcattggc caggataaac tgccgggctt
     3361 tgccggtatc tttggcttcg cctttgctta aaaataaaaa aggctcaact tccagggcgc
     3421 tggaggcctc cgccagcatt ttagcgttga ccagactggc tcgccagttg gttagctctc
     3481 cttctggatt gggaaagttg agatataagt attcagtgac aatcagttcc aaaacggcgt
     3541 cacctaaaaa ttccagccgt tcattgtggt ccagtttaaa gctggggtgc tcatttaaat
     3601 aagaccgatg aacaaccgat tgtttcagca ggtcaatatt tttaaatttg accttgaggc
     3661 ttttttgaag cttggagaaa tctttcatat aaattaactg aagaactaag gaactgagaa
     3721 actgagaaac agagaaacta aggaactgag aaacagagaa acagaggaac agagaaacag
     3781 agaaacagag gaacagagaa acagagaaac agaggaacag agaaac
//
