LOCUS       DASWKH010000001        22126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3332436, whole genome shotgun sequence.
ACCESSION   DASWKH010000001 DASWKH010000000
VERSION     DASWKH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 22126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22126
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3332436"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VGA92_00005"
     CDS             complement(<1..698)
                     /locus_tag="VGA92_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9844826.1"
                     /translation="MKIAVVGIGVAGGYLISRLKKDHEIVGFERMTEENHDSICAWGT
                     SRNGMKDLCAKSGINFDDFVIHHGKDLHIDMSNSQKFDIKLKGLVTYDKIGLIKEMAK
                     GAKIHYGVAPKLEDLEKEFDLIVDCTGFHRSYLPKIEKDFYLPTYQYKIQYDDKVPID
                     DFFVKPFPGMTGYFWYFPLSKNMAHIGAGDYKKNHVAETDKFFAKYGGKVTKTVGRPI
                     RLATPDMCEPFYHG"
     gene            complement(750..3620)
                     /gene="leuS"
                     /locus_tag="VGA92_00010"
     CDS             complement(750..3620)
                     /gene="leuS"
                     /locus_tag="VGA92_00010"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9844827.1"
                     /translation="MTDWNKIEEKWRAKWQQSKEFEIDPDARQKKFITVAYPYPNSPQ
                     HIGHGRTYTLADVHARFWRMRGYNVLFPMGFHYTGTPILGMAKRVQEGDRELIENFNT
                     LYHVPQDKIKEFVEPVKIADYFHAEIKQGMIEMGYSIDWRREFTTIDPVYKKFIEWQF
                     RKLREMNLVVQGSHPVGWCPKDQNPVSQHDTLGDVEPDFTEYILIKFHYDGMIIPTAT
                     LRPETIFGVTNLWINPSIRYKKIKVNDETWLVSPECVRKLEFLDKKITYLEDMDGSEF
                     VGKTVSVLDNKSIPILPASFVKSENGTGVVMSVPAHAPFDYQALVDIQKNGSKDIQVL
                     ASSLKPISIIKTQEYGEIPAKEVVDRFKIQDQNSPMLEEATKEVYAKEFYGGTLKENT
                     AKFAGLKVSEAKDAIREWLQSSRTSDILLELNNAPVRCRCGTECVVKILNNQWFLNYS
                     DKSWKEKANVCFEKMSILPNEIRPEFNYVVGWLKERACARQHGLGTPLPWDQGWIVES
                     LSDSVIYMAYYILAKYVNSGELVADNLSDQFFNYVLLGLGEPKQVSAQCKISEDILSN
                     IRNDFVYFYPVDARHSGRDLVPNHLTFFVLNHVAIFPEANWPKQIVVNGSVLMDGKKM
                     SKSMGNIIPLRQAIKDYGADPIRLAIIISAELLQDADFNLESVNSIKNRLENMLDECT
                     KYKPGITSKAESEDGWILARLQNLIVQATGAVEKMRLREALHHIIFSFESDLQWYLKR
                     VQAKQRTDVSGILHKILGTRIAMLSPFAPHAAEEMWERLGNSGLASRSSWPQPDAQID
                     QKSIQAEDLLMSVLDDIKNILKITKIISPQKIVLYTADRWKGKAYQKIVQSVVNGQTN
                     IGAIIKELIADKETENIKKDPDFVKKVVSGILAEPVEMRKLKAEMEPIDEAQIIKAEL
                     SSLVKKEFGVELQTFVESDPARYDPKNKARTARPFKPALLIE"
     gene            complement(3617..6331)
                     /gene="alaS"
                     /locus_tag="VGA92_00015"
     CDS             complement(3617..6331)
                     /gene="alaS"
                     /locus_tag="VGA92_00015"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9844828.1"
                     /translation="MNKEEILAKFSSEPERYYKVKLFSEHGFVRKSCSACKRFFWTLE
                     PDRNSCPDHADDTYSFIGNPPTSKRFDYTEAWRQVESFFVKNGHASISRYPVVCRWRD
                     DLYFTIASIVDFQRVMGSKVVFEFPANPLVVPQICLRFKDIENVGVTGRHFSSFCMIG
                     QHSIPNGKGYWKDECVDLDFRLLTEAFGINKNEIVFVEDVWVGGGSFGSSLEFYVRGL
                     ELGNAVFTEFQGELGNHTTLDQRIIDMGAGLERFAWITMGTPTAYDCCFGPITQKLLQ
                     RAGIDSDSKILTKYFTEIAKSLEKYHDLSQVRKAAIKASELSEEELNRIIIPLEGIYL
                     IADHLRTLIFAISDGALPSNVGGGYNLRMILRRIMATMDRLSLRLDLDELIDSHIDYL
                     KKTYPELEQYRSEVKTIIGIEIGRYHESKSRMEKISGSLKSQKKTLAVDDMIRLYESD
                     GITPDYLKEHQIISEIPDSFYSKLSELHQSEKKKAGEEFELKEIPETDLLFYKDDPVT
                     FEAKVLKVIKNKFVVLDRTSFYARGGGQEPDHGKINDSQVVDVTKHGNVVLHELSGTA
                     PKEGSTVRCEIDALRRQGITKHHTSTHIVNSSARNVLGSWVWQHSAFKEQEYGRLDIT
                     HHSSLTDEEIKKIEGVANSIIQKNLPVTIKEYERGQAEQTFGFRIYQGGVVPVKSVRI
                     VRIEDFDVEACGGTHVKRTGDLGLIKITKSERIQDGVVRLEFVSGQAAVQYVQKQESD
                     IFAIIRSLGSNREKLLKSFEHAMNDSESAKKKLKQLIKRTSGTIAKQSIESAKSFGAV
                     KFYSIIDEDLDEEFHIAVGEEAVKQNPAVIYCALIVRDSGIRIIVFAGEQATKVFKAG
                     DLVKEISAKLGGSGGGDARFGQGGGKDSANLADALAHAELLIKKTVNS"
     gene            6408..6917
                     /locus_tag="VGA92_00020"
     CDS             6408..6917
                     /locus_tag="VGA92_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844829.1"
                     /translation="MLGRKKELKEGDYVFAPRPDGEYQNIVFGVVTGIDGSKIGVSGL
                     IVNPVGLKNKVSQGKAGPRSAEILKTPTPENCILAFIYRTEHETFNQVLDLDEDKVVR
                     IQSKLYSVLDGWIRESLSEILNNVLSLPEGEERQQAKRLLKQRMETLYDKNLKQNLYS
                     VCRSLKILN"
     gene            6961..7269
                     /gene="rpl12p"
                     /locus_tag="VGA92_00025"
     CDS             6961..7269
                     /gene="rpl12p"
                     /locus_tag="VGA92_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HEX9844830.1"
                     /translation="MEYVYAALILHKLKKEINEANVSGIIKASGAEVSDAQVKALVSA
                     LADVNIEEAIKASPVAVAAAPAAAAAAPAAGGEEKKKDEATAAKSEEQAMEGLASLFG
                     "
     gene            complement(7333..8424)
                     /locus_tag="VGA92_00030"
     CDS             complement(7333..8424)
                     /locus_tag="VGA92_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844831.1"
                     /translation="MPIINISNIDISLARYAYTIEEIVDDLLKNKLDEEVKDFCKREL
                     GIKKVYKSYDLSKVKFDDTGYLAPHIELNDMYVESAKKALSPSRKPSDVGLLVTINDN
                     QQYLDPAPTVEIAIKLGLNKDVRTQNFQGMACASFSEALLNAAGHFALGHDSDVLVLI
                     GTYYTSWFLDRIKQIGHISMKNRKDFNNFIYFLIFSDTVAAALLSQSDGQIAQINTEF
                     VSSRKDTTPDGYKKATISLAPDSSFRVVFDMDVNSKILRERAASLSLENILHIKQTFP
                     TDFENVKVWGLHPAGRVFVDYIRERCGIERAKSQLTYDLMMETGNTGAASSLQLISES
                     VKRKTLRRGELGGIIDYGWEGADAFIYKA"
     gene            complement(8426..9364)
                     /locus_tag="VGA92_00035"
     CDS             complement(8426..9364)
                     /locus_tag="VGA92_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9844832.1"
                     /translation="MMPSKTGVELGIRAPSTAIKKAATIADTNMLDCYFVPETHPMFI
                     GVDAFEVLEAIANKVRNLTLGTGIVNVYSRDKKTMLQLASKIYRKSGGRFVLGLGTSA
                     PIIIENMYKMKFEKPLFRISGYTDYIKNQHNVPVYWAVVGDKITKLAAEHADGVIFFL
                     RPESEIKRSIKIIRNTLATVDRSYDQFDIVSIRPTYIEDHKNSEHIIRMTIANYVGAN
                     EFYSKPLEKIGYKKEVLAIRQNFAKHGLAEAARHVSNKMVRELATFGNAKECAEQIRQ
                     HADRTGIKTVVAGFDLPETGYNADFFLKLQKLAGRM"
     gene            complement(9432..10757)
                     /gene="lpdA"
                     /locus_tag="VGA92_00040"
     CDS             complement(9432..10757)
                     /gene="lpdA"
                     /locus_tag="VGA92_00040"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9844833.1"
                     /translation="MYDVVIIGGGPGGYSCAIRAAQLGGNICLIEKNGLGGTCTQRGC
                     IPTKFLHSAGDVIRRANSAKKNGLDANIQLNYKTLKSKMQATVEKLAQGIKLLLQSNG
                     VDLIEGKASIVSQNKILVNGVAIETKNIIIATGSHPVCLLGYEFDDRILSTDTILALD
                     ELPNSVAIVGGGYSGCEFACILNALGCKVSLIEAQSHLLPAQPQEIGDMIEKYMRMDG
                     INIITKSKVEKITENAVISNGEKIEAEKILVNIGRQPNTNADELNKIGVKFNRDGIIV
                     NEKMSTNIPNIFAIGDITGMYELAHVAAKQGEIAAQNAMGVESRMDYSVVPTCVFTYP
                     ELAFVGRFEGRTGEFPLAASAKASCLGDTRGMIKVFENDGILVGAFIISPHAGEMIGE
                     AALAIKMRLRPQDVFDTIHAHPTLPESLVDALRDIEGHAIHLPLKKNKS"
     gene            complement(10794..11528)
                     /locus_tag="VGA92_00045"
     CDS             complement(10794..11528)
                     /locus_tag="VGA92_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEX9844834.1"
                     /translation="MSLKFDKIRVLETGYNQASWNMALDEALLNKMDDTPVLRLYGWR
                     PPAVSIGYFQNMDQEVNVDRCKHCGIDVVRRMTGGGAVFHDSELTYSFLTKKYPQSII
                     ESYELICDAIIIAINKLGFVSKFSPLNDITINGRKVSGNAQTRKKNTLLQHGTILLNA
                     NIDEMFSVLKVPSEKLRDKMITDVKQRITVLDKTFDEVAAALKAGFSEKFDARLVMDK
                     LGEEEEADTKNLVREKYATHQWNWKR"
     gene            complement(11525..11803)
                     /locus_tag="VGA92_00050"
     CDS             complement(11525..11803)
                     /locus_tag="VGA92_00050"
                     /inference="COORDINATES: protein motif:HMM:NF021921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9844835.1"
                     /translation="MKEVNNTYKSQKLIKIFLKYDEANNTIHSIAITGDFFIHPEERI
                     DKLEADLAGARLDRDSIINVIEKSLSGSEIFGFNSQSMADAILGCLNK"
     gene            complement(11833..13257)
                     /gene="gcvPB"
                     /locus_tag="VGA92_00055"
     CDS             complement(11833..13257)
                     /gene="gcvPB"
                     /locus_tag="VGA92_00055"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9844836.1"
                     /translation="MKTIFEYDEETAGHLSDEDVLIRLPDGLTRNDLPLPNVSEINVI
                     RHYTNLSKKNFGVDLGFYPLGSCTMKYNPKINEDMAKLDGFSRLHPHSNEECTQGALE
                     LMWELEQHLKEITGMDAFSLQPAAGAQSELLGATIAKAHFSQRNEKRTKAIIPDSAHG
                     TNPATAAICKLDIVVIPTDPRGNMDMNEFKKALTADIAIVMLTNPNTLGLYEENILEI
                     CDLAHKNGTLMYCDGANMNALLGITRPGEQGFDMIHLNLHKSFSTPHGGGGPGAGVLG
                     VKSFLADYLPIPRVTKQNTKYMLDNDKPKSVGKIHSFYGNFGMLVRAYSYIMSWGSNI
                     RKVSEYAVLNANYLLSLLKDTYDLPYDRSCAHEFVISSKNLGEKSALNIAKRLLDYGF
                     HPPTIYFPLIVSEALMIEPTETESKETLDEFAAALKKIASELKENPQVVMSAPHNTPI
                     GRLDEVAAARKPNLRWGQSLQNNK"
     gene            complement(13254..14513)
                     /gene="gcvPA"
                     /locus_tag="VGA92_00060"
     CDS             complement(13254..14513)
                     /gene="gcvPA"
                     /locus_tag="VGA92_00060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX9844837.1"
                     /translation="MNYIPTTQKDRDKMLKQIGVNSINDLVKEFRPLLKASLNLPKPL
                     SEFEIRQHMQALASKNKILKYFVGAGSYNHYIPSALNHLLMRGEFSTGYTPYQPEISQ
                     GTLQAMYEFQSFMCILTGMDVANASLYDGASALAEAALLSASYNGRNRIFVKEGLHPQ
                     YLQVLRTYCDAANLEITNEIDDKTSCVIAQNPDFYGNVEDLTQLSDIAHKAGALFVVC
                     VVEPTSLAIIRAPADYGADIVVGEGQSFGIPINFGGPYLGFMAVKDFLLKKIPGRICG
                     MTTDSNGNKGFVLTFQAREQHIRRERATSNITTNQGLMALASTIYLALMGRNGLRTVA
                     RLSYGRAHLLQEKLEGLGFKTMNSKPFYNEFLVKTPKPAENILSALFENGMHGGLKIA
                     QDRLLICCTEMNTIADIERFASVVSES"
     gene            complement(14524..14901)
                     /gene="gcvH"
                     /locus_tag="VGA92_00065"
     CDS             complement(14524..14901)
                     /gene="gcvH"
                     /locus_tag="VGA92_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9844838.1"
                     /translation="MQDLKFTKEHEWAKISDSLAIIGISDFAQKQLGDIVSVELPKVG
                     SVFKQMQVMAIVDSIKASSDIYCPLSGQIVEINQGLREHPEWINQSPYDLGWMVKIKP
                     SNGSEFEKLMTKTQYDEFVGQIK"
     gene            complement(14923..15993)
                     /gene="gcvT"
                     /locus_tag="VGA92_00070"
     CDS             complement(14923..15993)
                     /gene="gcvT"
                     /locus_tag="VGA92_00070"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9844839.1"
                     /translation="MRTPFYEIHKSSNAKIVDFHGWEMPLEYSGIIDEHTNVRNSVGM
                     FDVSHMGRYQLKGSNSFDAIQNLVTNDVSKLSDNHGLYSPMCNEVGGIVDDVIVFRIN
                     KENFLIVVNASNREKDFQWISQHAKSVNIENVSDEISLLALQGPFAQSALQKIVEFDL
                     TQLKPFGIATTNIFGIKCMISRTGYTGEDGFEIFCDSSKPEIWNKLMQAGAEFNIKPI
                     GLGARDTLRLEAGLMLYGNDIDEDTTPLEAPLKWTVKWEKEFIGKKALVDKKPARKLV
                     GFDVKERRIARHGNKVMRNGAKCGFVTSGSFSPTFKKSIGLCFVPLDVVTDQLIEIDI
                     GGKSYEAKVCNTRFYRKEIKVL"
     gene            16048..16929
                     /gene="lipA"
                     /locus_tag="VGA92_00075"
     CDS             16048..16929
                     /gene="lipA"
                     /locus_tag="VGA92_00075"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9844840.1"
                     /translation="MELIRKPDWIKMRIPSGENYLRVKQTLGLQQLHTVCEEAKCPNI
                     SECWDAGTATIMIMGDTCTRGCRFCAVNTGKPTFLDAGEPQRVAEAIKDWGLQYVVIT
                     AVCRDDLADGGAGHMAKTVQAIKGLSPKIIVEPLIPDYNGDKESLKKITNSEPQVISH
                     NIETVERLTPKVRDGRASYEQSLFVLKTIKNIDSKIYTKSSMMLGLGENKEEVLQTLK
                     DLRAVGVDVVTMGQYLQPTIKHLPVVEYVPPKKFDEYKKEAEHMGFAYVVAGPFVRSS
                     YKASEPFIKNIIQLKTR"
     gene            complement(16950..17816)
                     /locus_tag="VGA92_00080"
     CDS             complement(16950..17816)
                     /locus_tag="VGA92_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX9844841.1"
                     /translation="MKENRTTYPQKKMQMYQQMQELPKKYKVTALVRMEKVRSSQLLP
                     LRKKFLGEVEVVSIKDKVVKKAFSALNIPGISKMVESLTGQCILMFTNMSPFKLNVLL
                     GKNKVMMYARGGDIASIDVMIPAKNTGIAPGPMLTEFKEAGVPTKIDQGTIWITKDTT
                     PVKKGGAVSEKLATLLQKLDIKTIEAGILLEAALEEGIQYKKEELIIDLAKYRELFAR
                     AHQEALSLSLEAAYITKDNIKQILAKVAQGSRSVAVEAGYVTDETKEPILQKANAQAR
                     SVASKAKGYTPA"
     gene            complement(17818..18474)
                     /locus_tag="VGA92_00085"
     CDS             complement(17818..18474)
                     /locus_tag="VGA92_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9844842.1"
                     /translation="MISEADLVKMIKEAKAADKERKFKQSVEMYVILKDIDVKKGFAM
                     NETIQLPKKLSTPTTVCIMAGGDMGLKAKNANADRVISGDEINSLAADKRKARKFINN
                     YDFFLADTQLMTTVGKVLGQLLGPRGKMPVPVPFNAPIDSFLDRFRSSIRVKVKNSLS
                     LSCKIGDESMSDEDLAANAHAILNAIEKKLPSGERNIRKIIIKTSMGKPIKQLQQARK
                     "
     gene            18640..19098
                     /locus_tag="VGA92_00090"
     CDS             18640..19098
                     /locus_tag="VGA92_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9844843.1"
                     /translation="MHKLDNLDMKLLYELTKDGSISVPNLSKKLGINASVLYSRIKRL
                     TKKKLIKKFTIVIDEAQLGIGVKATIGINRDPKQKDAIHRELLQTPEIASISEVTGRF
                     DIMVGVHAENLERLHNIVIDKIGKIEGIQSTETFVELQKTDKEPSYLVSK"
     gene            complement(19100..19582)
                     /locus_tag="VGA92_00095"
     CDS             complement(19100..19582)
                     /locus_tag="VGA92_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9844844.1"
                     /translation="MSDTQTISALVTGGQASAGPPLGPALGPLGVNIMEIINTINEKT
                     SDFEGMKVPVTVSVNIKTKKWEVIVGIPSAAALLLKEAGIQKGSGTSGSTWVGDINVD
                     SLVKVAKAKLESSYASSVKSVAKEVAGTCVSLGIKIEGKSPKEFTAEVNAGKWDDKLK
                     "
     gene            complement(19617..20075)
                     /locus_tag="VGA92_00100"
     CDS             complement(19617..20075)
                     /locus_tag="VGA92_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HEX9844845.1"
                     /translation="MSQEIKSHLFAVRTTGGQEKVVMNLLQNKIKTNKINILSVLIVD
                     NLKGYIVIEAKDANAAFDALQGIRHIRGQLRGELEFKDIEGYLVTKTSAPQIAIDNIV
                     EIIGGPFKGMKATVTRVDNDKQEATVILLDAPYQLPVTVDSNYLKISSAS"
     gene            complement(20082..20246)
                     /locus_tag="VGA92_00105"
     CDS             complement(20082..20246)
                     /locus_tag="VGA92_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HEX9844846.1"
                     /translation="MDLKVTLKNMANTMKLARKSDKDDYMQHLRLVVLGVASIGTIGF
                     IIQFVFSVFR"
     gene            20286..21062
                     /locus_tag="VGA92_00110"
     CDS             20286..21062
                     /locus_tag="VGA92_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX9844847.1"
                     /translation="MELLVAYQKDPAGHNMAKFISKNMKNDGDVFRGKNFDLVIIPSP
                     AISADWLEEKYHYDGFVFLSKHAAQSGDLALTCHSTGNFSDAQFGGNKRQIAVPHPHL
                     QKSYLQALWQNRKNFEEFQITIEATHHGPTALSKPALFIEVGTTEKQWNDESLCNSVA
                     KLVLQVLSEPPKANPVAICFGGTHYPDKFTKELIKGKHALGTVVPKHALEFVDEEMFS
                     HILNQNRQAETALLDWTGLGKYKQKVFDLLKTTDLKVIKL"
     gene            21059..21361
                     /locus_tag="VGA92_00115"
     CDS             21059..21361
                     /locus_tag="VGA92_00115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX9844848.1"
                     /translation="MSRLEHRVYQKLLKVPKGKVTTYGELANAVGLENGQRIIGQIMN
                     KNPFPVIIPCHRVVKSDGKIGGYYYGEKVKTKMLSDEGVSVKNGKIQDWEKTVFRF"
     gene            complement(21358..21813)
                     /locus_tag="VGA92_00120"
     CDS             complement(21358..21813)
                     /locus_tag="VGA92_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HEX9844849.1"
                     /translation="MVVNLKAKKRLVSRVAGVGLSRIKFDSDSSDDIADAITRDNIRS
                     LISAGIITIKAAKGTSRGRAKFKKIQKGKRGTKTGSKKGRKGARVGKKRVYVAKVRSL
                     RHRLKVSKDRKEITNPQFWELYRKIGGNTVRNIAHLRSLIDEVKAKQKT"
     gene            complement(21797..>22126)
                     /locus_tag="VGA92_00125"
     CDS             complement(21797..>22126)
                     /locus_tag="VGA92_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HEX9844850.1"
                     /translation="SWRYRRIGLAWRKPKGIDNHQRRQKFRGRPGLVKIGYGGPKLVR
                     GLHPSGFTDNLVYNVNDLAKLDPKNDGVRIAHSVGKKKRLDIVTKAIEKKFKVFNARV
                     KTSGSKS"
BASE COUNT         5820 a         4861 c         4330 g         7115 t
ORIGIN      
        1 ttgccgtggt agaacggctc acacatgtct ggagttgcaa gtctgattgg ccttccaacc
       61 gtttttgtta ccttgccgcc gtattttgca aagaacttgt ctgtttctgc tacgtggttt
      121 ttcttgtaat cccctgcacc aatgtgagcc atattcttac tcagcgggaa ataccagaaa
      181 taaccagtca tgccaggaaa cggctttaca aaaaagtcat caataggtac cttgtcgtca
      241 tactggatct tgtactgata tgttggaagg taaaagtcct tttcaatttt gggcagatag
      301 cttcgatgaa atcccgtaca gtctacaatc agatcgaact ctttttccaa atcttcaagc
      361 tttggcgcaa caccgtaatg gatcttggct ccctttgcca tctccttgat taggccaatt
      421 ttatcatatg ttacaaggcc ttttagcttg atgtcaaatt tctgactgtt actcatgtca
      481 atatgcaagt ctttgccgtg gtgaatcaca aaatcatcaa aattaattcc agattttgcg
      541 caaagatcct tcattccatt tctggaggtg ccccaagcgc agatggaatc atggttttcc
      601 tcagtcatgc gctcaaagcc tactatctcg tgatcttttt tgagtcgcga aatcagatat
      661 ccacccgcta caccaattcc aacaacggct attttcaatt tactggaaat ttcgtatcgc
      721 ctaataaata cacttttgtg aatgcaggat cattctatga gtaaggcagg cttgaatggt
      781 cttgcagtcc ttgccttgtt ttttggatcg tatcttgcag gatccgattc tacaaaagtt
      841 tgcaattcta cgccgaactc tttttttacc aacgaggaaa gttccgcttt tatgatttga
      901 gcttcatcta ttggttccat ctcagctttt aatttgcgca tctcaacagg ctctgccaaa
      961 atcccactaa cgactttttt tacaaaatct ggatcctttt tgatgttctc agtttccttg
     1021 tctgcaatta gttctttgat tattgcacca atgtttgtct ggccattgac gacactttgt
     1081 acaatttttt gataggcctt gcctttccaa cgatcggctg tgtacaaaac aattttttgt
     1141 ggactgataa tcttggttat cttcaaaatg tttttgatgt cgtcaagtac tgacattaag
     1201 agatcttcag cttgaattga tttttgatca atttgggcgt ctggctgggg ccaagatgat
     1261 ctagaagcta atccggaatt gccaagcctt tcccacatct cttcggcagc atgcggagca
     1321 aacggagaaa gcatcgcaat ccttgtgcca agtatcttgt gcaaaattcc agatacgtcg
     1381 gttctttgct ttgcttggac acgcttgagg taccactgca gatccgactc gaagctgaaa
     1441 atgatgtgat gcagtgcctc ccttaacctc attttttcta cggccccagt tgcctgtacg
     1501 atgagatttt gaagtcgcgc caaaatccaa ccatcctccg actctgcctt agaagtaatg
     1561 ccgggtttgt attttgtaca ttcatcaagc atgttctcaa gcctattttt tatgctgttt
     1621 acagactcta aattaaaatc agcatcttgt aatagttctg cagaaataat tatggcaagt
     1681 ctgatcgggt ctgcaccata atctttgatt gcctgcctta gtggaatgat gtttcccatg
     1741 gactttgaca tttttttacc atccattaga acagaaccgt ttactacaat ctgctttggc
     1801 caattcgcct ctggaaatat ggcaacatgg tttagaacaa aaaaggtcag atggtttggc
     1861 acaaggtcgc gtccagaatg ccttgcatca actggataaa aatacacaaa atcgtttcgt
     1921 atgttgctta gaatgtcctc tgatattttg cactgtgcag aaacctgttt tggctctcct
     1981 aagcccagca aaacatagtt gaaaaattgg tccgagaggt tgtctgcaac aagttctcca
     2041 ctgtttacat attttgccaa aatatagtat gccatgtaaa ttacagaatc cgataggctt
     2101 tccacaatcc agccctgatc ccatggcagg ggggtgccaa gtccatgttg tcttgcacac
     2161 gccctttctt ttagccagcc gacaacatag ttaaattcag gcctaatctc atttggcaaa
     2221 atactcatct tctcaaaaca cacattggct ttttctttcc atgatttgtc agaataattc
     2281 aaaaaccact ggttgtttag gatctttaca acacactctg tgccacacct acagcggact
     2341 ggggcattgt ttagctcaag caaaatatct gaagtcctag agctttgaag ccactcccgt
     2401 atggcatcct ttgcctcgga aactttgagg cctgcgaact tggctgtatt ttcctttaat
     2461 gtcccgccat agaactcttt tgcgtacact tcctttgtgg cctcctcaag catggggctg
     2521 ttttggtcct ggatcttgaa cctatctacc acttcctttg ctggaatttc gccatattct
     2581 tgggtcttga ttatggaaat tggttttagc gagctggcaa gcacctgtat gtctttactg
     2641 ccgttttttt gtatgtctac tagggcttgg tagtcaaatg gtgcgtgggc tggaaccgac
     2701 atcacaaccc cagtaccgtt ttcactttta acaaaacttg caggaaggat tgggattgat
     2761 ttgttatcta aaacgctaac ggtttttcca acaaactccg agccgtccat gtcctcaagg
     2821 taggttattt ttttgtcaag aaattcaagt ttgcgcacac attctgggct gactagccac
     2881 gtctcgtcgt ttactttgat ttttttgtat cttattgatg gattaatcca aagatttgtc
     2941 acgccaaaaa ttgtctcagg tcttaaagtt gcagttggga tgatcatccc atcataatga
     3001 aacttgatca aaatgtattc agtaaagtct ggctctacgt ctccaagtgt gtcgtgctgg
     3061 gaaactgggt tttgatcctt tggacaccac ccaacggggt gtgatccctg tactaccaaa
     3121 ttcatctccc ttagcttgcg gaattgccat tcgatgaatt ttttgtagac aggatcaatt
     3181 gtcgtaaatt ccctacgcca gtcaatggag tagcccatct ctatcattcc ttgctttatt
     3241 tcggcgtgaa aataatcggc aattttgact ggttcgacaa actcttttat tttgtcttgc
     3301 ggaacgtggt aaagcgtatt aaaattttca attaactccc tgtcgccttc ttgaactcgc
     3361 tttgccatcc caagaatcgg ggttccagta taatgaaacc ccatcgggaa aagtacgttg
     3421 tagccacgca tgcgccaaaa tcgtgcgtgg acatcagcta gcgtataggt tcttccatgg
     3481 ccaatgtgct ggggtgagtt tgggtatgga taggcaacag tgatgaattt tttttgcctt
     3541 gcatccgggt ctatctcaaa ttcttttgat tgctgccact tggctctcca cttttcttct
     3601 atcttgttcc aatctgtcat gagttcactg tctttttgat taataattcc gcatgtgcta
     3661 gcgcatcggc aagatttgcg gaatcttttc ctcctccctg gccaaacctt gcatctccac
     3721 caccagagcc gccaagcttt gcagagatct ctttgactag atccccggct ttgaagactt
     3781 tggtagcctg ctctcctgca aaaacaataa tcctaatccc tgaatccctc acaatcaatg
     3841 cgcaataaat gactgcgggg ttttgtttga ctgcttcttc gccaactgca atgtggaatt
     3901 cttcgtcaag gtcctcatct attatagaat aaaactttac ggcgccaaaa ctctttgcag
     3961 attcaattga ctgttttgca atggttccac tggtcctttt gattagctgc tttagctttt
     4021 tctttgcaga ctctgaatca ttcattgcat gctcaaatga ttttagaagc ttttctctgt
     4081 ttgagccaag tgatcttatt attgcaaaaa tgtcgctttc ttgtttttgc acatattgca
     4141 ccgctgcctg gcctgaaaca aactccagtc ttacaacacc atcttgtatc ctctcagatt
     4201 ttgtaatttt tattagtcca agatcgcctg ttctttttac atgagttcct ccgcaggctt
     4261 cgacatcaaa gtcctcaata cgtacgattc gcactgattt tacaggaaca actccgcctt
     4321 gatatattct aaaaccgaag gtctgctctg cctgcccccg ctcgtattct ttgatggtga
     4381 ctgggagatt tttttgaatt atcgagttgg ccacaccttc aattttctta atctcttcgt
     4441 cagttaggct tgagtgatgt gtaatgtcta gtctcccata ttcttgctct ttgaatgcag
     4501 agtgttgcca gacccacgag ccgagaacat ttcttgcaga ggaatttaca atgtgggtac
     4561 tggtatgatg ttttgtgatg ccttgtcgtc tcaaagcatc tatttcacat ctgacagtgg
     4621 agccttcttt tggggctgtc ccacttagct catgcaatac gacgtttcca tgttttgtta
     4681 catcaactac ttgggaatcg tttatcttgc cgtgatctgg ttcctgtccg ccaccccttg
     4741 cataaaatga cgttctgtct aggacaacaa acttgttttt gattactttg aggactttgg
     4801 cttcaaacgt cacaggatca tctttgtaaa agagcagatc tgtttctggg atttctttga
     4861 gctcaaactc ttcaccggct ttcttttttt cggattggtg gagctcagat agcttgctgt
     4921 aaaagctgtc tggtatttct gaaattatct ggtgttcttt gagataatca ggcgtgattc
     4981 catctgattc gtagaggcgt atcatatcat ctactgcgag ggttttcttt tggcttttta
     5041 ggctgcctga tattttttcc attcttgatt tggattcatg gtaacggcct atctcaattc
     5101 caattattgt cttgacctcg cttcgatact gctcgagttc gggatatgtc ttttttagat
     5161 aatcaatgtg gctgtcaatg agctcatcta ggtcaagcct taagctcagt ctgtccatcg
     5221 tagccattat tcttcttagg atcattctta ggttgtagcc tccgccaacg ttgcttggaa
     5281 gggccccatc agagatggca aaaatcaagg ttcgaagatg atccgctatt aggtaaatgc
     5341 cttccagcgg gattatgatc ctgtttagct cttcttctga aagttctgag gccttgattg
     5401 cggcctttct tacctgcgag agatcatgat atttttccag actctttgct atttctgtaa
     5461 agtattttgt tagaattttc gagtcagaat cgattcctgc ccgctgtaat agtttctgcg
     5521 ttatcggacc aaaacagcaa tcataagcag tgggggtgcc catcgtaatc caggcaaacc
     5581 tttcaagacc tgctcccatg tcgatgattc tctgatcaag tgttgtgtga tttccaagct
     5641 ctccttgaaa ttctgtaaag accgcgtttc caagctcaag acctcttacg taaaactcta
     5701 gtgaggagcc aaacgatcct ccacctaccc agacgtcttc gacaaaaacg atttcatttt
     5761 tgtttattcc aaacgcttcg gtcagtaatc taaagtcaag atctacacac tcatccttcc
     5821 agtagccctt gccatttggt atgctgtgct gcccaatcat gcaaaagctc gaaaaatgtc
     5881 tgccggtaac gccgacattc tcaatgtctt tgaatcgaag acatatctgt ggtaccacaa
     5941 gcgggtttgc tggaaactca aagacaacct ttgagcccat gactctttga aagtctacta
     6001 ttgatgcgat tgtaaagtaa aggtcgtctc gccatctgca cacaactgga tacctgctaa
     6061 tggaagcatg gccgtttttt acaaaaaatg actcgacttg tcgccatgcc tcagtatagt
     6121 caaatctttt gcttgttgga gggttgccga taaacgaata ggtgtcatct gcatgatcag
     6181 ggcaagaatt tctgtctggc tccaaagtcc aaaaaaatct cttacatgca gagcatgatt
     6241 tacgaacaaa tccatgctcg gaaaacaatt ttaccttata gtaacgctca ggctctgaag
     6301 aaaacttggc aagaatttct tccttgttca tcttctgaga ttcgaattaa ccaatattaa
     6361 gcaattcgct cggataaatt aaatacaata ttgtttgcat ttttctcatg cttggtcgca
     6421 agaaggaact aaaagaaggc gactacgttt ttgcaccaag acctgacggt gaatatcaaa
     6481 acattgtctt tggggtggtg actggaattg acggttcaaa aataggcgtc agcggattaa
     6541 tcgtaaatcc agtgggtcta aaaaacaaag tctctcaagg aaaggcaggg ccaagatctg
     6601 ctgaaattct aaaaacacca actcctgaaa actgtatttt ggcatttatt tacagaacag
     6661 aacatgagac tttcaaccag gtcttagatc tggacgagga caaggttgtc cggatccaat
     6721 caaaacttta ttccgtctta gatgggtgga taagagagtc gctatctgag attctcaaca
     6781 acgtcctctc acttcctgaa ggagaagaac gacaacaagc taaacgactc ttgaaacaac
     6841 gaatggagac gctttacgat aaaaatctaa aacaaaacct ctattctgtt tgtcgaagct
     6901 tgaaaattct aaactaaccc gcatagtttt atattatccc caaaagacag gcacgataca
     6961 atggaatatg tttacgccgc attaattttg cacaaactca aaaaggaaat taatgaagct
     7021 aatgttagtg gcattatcaa agccagcgga gcagaagtta gtgatgctca agtcaaagca
     7081 ctggtatcag cacttgccga tgtaaacatt gaagaggcaa tcaaggcatc accagtagcc
     7141 gttgcagcag cgccagcagc agccgcagca gcgccagcag ccggtggcga agagaaaaag
     7201 aaagacgagg ctacagcagc caagtcagaa gagcaagcaa tggaaggcct cgcctcattg
     7261 ttcggctaga aaatttctat tactatacat tcatttttta aaaaatgctt ttttataaaa
     7321 ccggtctgta gctcacgctt tgtaaataaa tgcgtcagct ccctcccacc cataatcaat
     7381 aattccacca agttcgccgc ggcgtaaggt ctttcgtttc accgactcgc tgatcagctg
     7441 caaagaacta gctgctccgg tgtttccagt ttccatcatc agatcatagg ttagttgtga
     7501 tttggccctt tctatgccgc aacgctctct gatataatca acaaacaccc ttcctgccgg
     7561 gtgaagcccc cagaccttga cattttcaaa gtctgttgga aatgtctgct tgatgtgcaa
     7621 aatgttttcc aaagaaaggc ttgcagccct ttcccttaga atttttgaat ttacgtccat
     7681 gtcgaagact accctaaacg agctgtctgg tgcaagactt attgtcgcct ttttgtatcc
     7741 atccggggtg gtgtctttgc gtgaagacac aaactctgtg tttatttgcg caatctgtcc
     7801 gtccgactgt gatagtaagg ctgctgccac cgtgtcgctg aaaatcaaga aataaataaa
     7861 attgttaaag tcctttctat tcttcattga gatgtggcca atttgtttta tcctgtccaa
     7921 aaaccaactt gtataatacg tgccaattaa aactagcacg tcgctgtcgt ggcctaaggc
     7981 aaaatgccca gctgcattga gaagggcctc tgagaacgaa gcacacgcca ttccttgaaa
     8041 attctgtgtc cgaacatctt tgtttaaacc caattttatt gcaatttcaa ctgtgggtgc
     8101 tgggtctaga tactgttggt tgtcattaat ggtaacaagc aatccgacat cgcttggttt
     8161 tcgagatgga gacaacgcct tcttggcact ttcaacatac atgtcattga gttcaatatg
     8221 tggtgcaaga tagccagtgt catcaaattt taccttggat aaatcatatg atttgtagac
     8281 tttttttatg ccgagttctc ttttgcaaaa atctttgacc tcctcgtcca gcttgttttt
     8341 gagcaaatcg tctacaatct cttctatggt gtaggcgtat ctggcaaggc tgatgtctat
     8401 gttggagata ttaataattg gcatatcaca ttctgcctgc tagtttttgt aattttaaaa
     8461 aaaagtcggc gttgtaacct gtttctggca aatcaaaacc tgctacgaca gtttttattc
     8521 cagtcctatc tgcatgctgc cttatttgtt cggcgcattc cttggcgttg ccaaaagtgg
     8581 ccagttctct taccatttta ttgctcacat gccttgctgc ttcggccaaa ccgtgttttg
     8641 caaagttttg ccgtattgcc agcacttctt ttttgtagcc tattttttct agcggttttg
     8701 aataaaactc gtttgcacca acatagttcg ctattgtcat acgtatgata tgttcagaat
     8761 ttttatgatc ttcaatatat gtgggcctaa tcgaaacaat gtcgaactga tcgtagctcc
     8821 tgtcaacagt ggcaagcgta tttcttataa ttttgatact gcgtttgatc tcactttcag
     8881 gcctgaggaa aaatatgact ccgtctgcgt gctccgcagc aagttttgtt atcttgtctc
     8941 caactactgc ccagtaaacg ggtacattgt gttgattttt tatgtaatca gtatagccgc
     9001 taattctaaa aagtggtttt tcaaatttca ttttgtacat gttttcaata atgatcggag
     9061 cacttgttcc aagtccgaga acaaatcttc cgcctgattt gcgataaatc ttgctagcaa
     9121 gttgcagcat tgttttttta tctctagagt acacattaac aattccagtg cccagtgtca
     9181 gattccgaac tttgttcgca attgcctcaa gaacctcaaa tgcatcaact ccgataaaca
     9241 ttggatgggt ttctggaaca aaatagcaat ctagcatgtt tgtgtctgcg attgtagcgg
     9301 ctttttttat tgcagttgat ggcgccctta tgccaagctc tacacctgtt tttgaaggca
     9361 tcatagagct tgaactattt ggttctcata aatgctttaa aattgcattt aggatttgac
     9421 aagtattttg cttaggactt attcttcttt aagggaaggt gtatggcgtg cccttcaata
     9481 tctcttagcg catcaacaag cgattctgga agggtagggt gggcatgtat ggtatcaaac
     9541 acgtcttgcg gccttaatcg catctttatt gcaagtgctg cctcaccaat catttcacct
     9601 gcatggggag aaattatgaa tgcgccaacc aatatgccgt cattttcaaa aaccttgatc
     9661 atcccccttg tgtctccaag gcagcttgct tttgcactag ctgcaagcgg gaactcgccc
     9721 gtcctacctt caaatctgcc tacaaatgcc agctcggggt atgtgaatac gcaagtaggg
     9781 actacactat agtccattct gctttcaacg cccatcgcgt tttgagctgc aatctcgcct
     9841 tgttttgctg ccacatgtgc aagctcgtac atcccagtta tgtcgccgat agcaaatatg
     9901 tttggaatat ttgttgacat cttctcattt acgatgatgc cgtccctgtt gaattttaca
     9961 ccgatcttgt ttaattcatc cgcatttgtg ttgggttgtc ttccaatgtt tacaagtatt
    10021 ttttctgcct ctattttttc cccattagaa attactgcat tctcagttat tttttctact
    10081 ttggattttg tgatgatgtt gatgccgtcc attctcatat atttttcaat catgtctcca
    10141 atctcttggg gctgagcagg cagtaggtgg ctttgtgcct ctattaaaga aaccttgcat
    10201 ccaagcgcat tcaaaatgca ggcaaactca caaccgctgt agcctccacc aacaatagct
    10261 acggagttgg gcaactcatc caacgctaaa attgtatcag tcgaaagtat ccgatcatca
    10321 aactcgtatc caagcaagca aacaggatgg cttccagtgg caataatgat gtttttggtc
    10381 tctatcgcaa ctccattcac cagaatttta ttttgcgaga ctatgctcgc tttgccttct
    10441 atcagatcaa cgccattact ttgaagaaga agttttatcc cctgggcaag tttttcaaca
    10501 gttgcctgca ttttggactt taaggtcttg taatttagct ggatattggc atccaagcca
    10561 ttcttttttg cagaatttgc cctccttatc acatctccag cagaatggag aaacttggtt
    10621 ggaatgcagc ctcgttgagt acatgttcct cccaaaccgt tcttttctat taggcaaatg
    10681 tttccgccaa gctgggcagc cctgatggca caagaataac ctcctgggcc tccgcctatg
    10741 attacaacat cgtacacgag tcagaatcaa aactcacagc aataaatctt tgattatctt
    10801 ttccaattcc attggtgggt tgcgtatttt tctctaacta ggttttttgt gtccgcttcc
    10861 tcctcctcac caagtttatc cattactagt ctggcatcaa atttttcgct aaatccagcc
    10921 tttagggcag ctgctacctc atcaaacgtt ttgtccaaga ctgtgattct ttgtttcaca
    10981 tcggtgatca tcttgtctct tagcttttca gaaggaacct tcaatacaga aaacatctcg
    11041 tcaatatttg cgtttaataa tattgtgccg tgctgcaata gcgtattttt tttccttgtc
    11101 tgggcatttc ctgaaacttt tcttccatta atggttatgt catttaatgg ggaaaattta
    11161 gatacaaacc caagcttatt gattgcaatt attattgcgt cgcaaatcaa ttcatacgat
    11221 tcaattatgc tttgcggata tttttttgtc aaaaatgaat aggtcagctc agaatcatga
    11281 aaaactgctc cgcccccagt catccttctg accacatcga tgccacagtg tttgcatctg
    11341 tctacgttaa cttcctgatc catgttttgg aagtagccaa tcgatactgc ggggggcctc
    11401 catccgtaga gcctcagcac cggcgtatcg tccatcttgt tgagtagtgc ctcgtcaagt
    11461 gccatattcc acgatgcttg gttgtagcct gtctctaaaa ccctgatttt atcaaacttc
    11521 aaactcattt gtttaaacat ccaagaattg cgtctgccat agattgcgaa ttaaagccaa
    11581 atatttctga accactcagg ctcttttcta ttacgttaat aatggaatct ctatctagtc
    11641 tggcaccagc taaatcggct tcaagcttgt ctattctttc ttctggatga ataaaaaaat
    11701 caccagtaat ggctatcgag tgtattgtat tattggcttc atcatatttt agaaaaatct
    11761 tgattagttt ttgtgatttg tatgtgttat ttacttcctt catggcaaaa cggtcttgac
    11821 tttattgcta gatcatttat tattttgcaa agactggccc catctaagat ttggctttct
    11881 tgcagctgcg acttcgtcta atctgccaat gggtgtattg tgaggtgcac tcattacaac
    11941 ttgggggttt tcttttagct cagacgctat tttctttagg gcagcagcaa attcatccag
    12001 tgtttctttg ctttctgttt cagttggctc aatcatcaac gcctcactaa caattagtgg
    12061 gaaatagatt gttggcggat gaaatccgta atctaaaagc cttttagcaa tgttcagtgc
    12121 gctcttctca cccaagtttt tggaagagat gacgaactcg tgtgcacaac ttcggtcata
    12181 gggaagatca taggtatcct ttagcaaact gagaaggtag ttggcattta gaactgcata
    12241 ctcggaaacc ttccttatgt ttgagcccca cgacatgatg taagaatagg ctctaacgag
    12301 catgccaaaa tttccataga atgaatggat cttaccaaca gacttgggtt tgtcattatc
    12361 cagcatgtat ttggtatttt gtttggttac tctaggtatt ggcagatagt ctgccaaaaa
    12421 tgactttact cctagtactc cagcaccagg accaccaccg ccatgcggtg ttgaaaaact
    12481 cttgtgcaaa ttcaaatgaa tcatatcaaa gccttgttcc ccaggtctgg ttatcccaag
    12541 caatgcgttc atgtttgcac catcgcagta catcagtgtg ccgtttttgt gtgcaagatc
    12601 acaaatctcc aaaatatttt cttcataaag ccccaacgtg ttgggattgg ttagcataac
    12661 tatcgcgatg tcggcagtaa gtgccttttt gaattcattc atgtccatgt tgcccctcgg
    12721 gtcggtggga atgacaacaa tgtctaactt gcaaatggcc gcagtggcgg gattggtacc
    12781 gtgggcagaa tcaggaatta tggccttggt tcttttttcg tttctttgac taaagtgggc
    12841 ttttgctatt gtagccccca aaagctcgct ttgtgccccg gccgcaggct gcagggaaaa
    12901 cgcgtccata cctgttatct cctttaggtg ttgttctaat tcccacatga gctcaagtgc
    12961 cccctgtgta cactcctcat tggaatgagg gtggagtctg ctaaaaccat ctagttttgc
    13021 catgtcctca ttaatcttcg gattgtattt catcgtgcaa gacccaagag gatagaatcc
    13081 aagatcgact ccaaagttct ttttggacaa attggtatag tgcctaatca cattaatctc
    13141 actgacattt ggaagtggta gatcgtttct tgttagacca tcaggcagtc ttattaaaac
    13201 atcttcatca gaaagatgcc cagctgtttc ttcatcatac tcaaatattg tcttcatgat
    13261 tcactcacta ctgatgcaaa gcgttctatg tctgcaattg tattcatttc agtgcagcaa
    13321 attagtaatc tatcttgggc aattttgaga cccccatgca tgccgttttc aaacaacgct
    13381 gaaagaatat tctcagctgg ctttggtgtt ttcacaagga attcgttgta aaacggcttg
    13441 ctgttcattg tcttaaaccc taggccttcc agtttttcct gcaacaggtg tgcccttcca
    13501 taagaaagcc tcgcaaccgt tctaagtcca ttcctgccca ttagtgccaa gtagatggtt
    13561 gaggccaacg ccatcaaacc ttggtttgtt gttatgttgc ttgtagccct ttctcttctg
    13621 atgtgttgtt cacgagcttg aaatgttagt acaaaacctt tgtttccgtt tgaatcagtt
    13681 gtcatgccac aaatcctgcc tggaattttt ttaagaagaa aatccttgac tgccatgaat
    13741 ccaaggtatg gtcccccaaa gtttatcggt ataccaaatg actgtccctc accaactacg
    13801 atgtctgctc cataatctgc aggtgctctt attattgcaa gagatgttgg ttcaacgaca
    13861 caaacaacaa acaatgcgcc ggccttgtgt gcaatgtctg ataattgtgt caggtcctca
    13921 acgttgccgt aaaagtctgg attctgagct atcacacatg atgttttgtc gtcaatttcg
    13981 tttgtgatct ctagattggc agcatcgcaa tatgtccgta gaacctgcag gtattgtggg
    14041 tgtaatcctt ccttgacaaa aatacggttt ctcccgttgt aagatgcgga aaggagtgct
    14101 gcttctgcta gggcagacgc accatcatac agggatgcat ttgcaacatc cattccagta
    14161 agaatgcaca taaaggattg gaactcgtac attgcctgta gtgttccttg gcttatctct
    14221 ggctggtatg gcgtatagcc agtagaaaat tcccctctca ttagcagatg attgagggcc
    14281 gaagggatgt agtgattgta ggagccggcc ccaacgaaat acttcaatat tttatttttg
    14341 ctggccaaag cctgcatgtg ctgtctaatc tcaaactcgc tcaaaggttt tggaagattc
    14401 aggctcgctt tgaggagggg cctgaattcc ttaactaaat catttatcga gtttacacca
    14461 atttgtttta gcatcttgtc tctgtctttt tgggttgtcg gtatgtaatt cataattgat
    14521 cttttacttt atttggccaa cgaactcgtc gtattgcgtt ttagtcatca gcttttcaaa
    14581 ctcactgcca tttgacggtt ttatctttac catccaaccc aagtcataag gagattgatt
    14641 tatccactcg gggtgctcac gaagtccttg attgatttct actatttgac cgctcaaggg
    14701 gcagtagatg tccgaagacg cttttatcga gtcaactatt gccatcactt gcatttgttt
    14761 aaagacgctt ccaacttttg gtaattctac tgaaacaatg tcccccagtt gtttttgagc
    14821 aaaatctgaa atgccaataa ttgccaaact gtcactaatt ttggcccact catgttcttt
    14881 agtaaatttg aggtcttgca acacttgctc tccagtttga cattataaaa ccttaatttc
    14941 ttttctgtag aatctagtgt tgcaaacttt agcttcatag gacttgccgc caatatcgat
    15001 ttcaattaat tgatcggtta caacatctaa tggcacaaaa caaagaccga tagatttttt
    15061 aaaagttgga gaaaaacttc cacttgtcac aaaaccgcac ttggcaccat tcctcatcac
    15121 tttattacca tgtcttgcaa ttcttctttc tttgacatca aaaccaacaa gctttctggc
    15181 tggttttttg tcaacaagtg cttttttccc tatgaattcc ttttcccact tgacggtcca
    15241 ttttagcggt gcctcaagag gcgttgtatc ttcatcaata tcatttccat aaagcatgag
    15301 gcctgcttct agcctcagag tgtctcttgc gccaaggcca attggcttga tattaaattc
    15361 tgctccggcc tgcattaact tgttccaaat ttctggtttt gatgagtcac aaaatatctc
    15421 aaacccatcc tcgccagtat atcccgttct tgatatcatg catttgattc caaaaatgtt
    15481 tgtagtagct atgccaaatg gttttaactg tgtcaggtca aactcgacaa ttttttgtag
    15541 tgctgattgt gcaaaaggtc cctgtagcgc aagtaaggag atctcatcac ttacattttc
    15601 gatatttaca gatttggcat gttgagagat ccattgaaag tctttttctc tgtttgaagc
    15661 gttaactaca attaggaaat tctccttatt gattctaaat actatcacgt cgtcaactat
    15721 gccacctacc tcattacaca tgggagaata caacccatga ttgtcgctga gttttgatac
    15781 gtcgtttgta accaaattct gtatggcatc aaaagagttt gaccctttga gttgatagcg
    15841 acccatgtgt gatacatcaa acatcccaac agaattccta acgttagtgt gctcatcaat
    15901 tatgcctgaa tactccaaag gcatctccca tccatgaaaa tctactattt ttgcattcga
    15961 tgatttgtga atctcgtaaa atggtgtccg caaactaatt tcgtaatgcc ttaactctta
    16021 ttattgtgta tggcaatttt ttagaatatg gagttgattc gaaagcctga ctggatcaaa
    16081 atgaggatcc cgtcgggaga aaattatctt agagtcaaac agacacttgg actgcagcag
    16141 cttcacaccg tttgtgaaga agcaaaatgt ccaaacatat cagaatgctg ggatgctgga
    16201 accgctacga taatgataat gggtgatacg tgcacaagag gatgcagatt ctgcgccgtc
    16261 aatactggaa aacccacatt tcttgatgca ggggaaccgc aaagagtggc agaagcaatt
    16321 aaggattggg gcttgcagta cgtggtaatt actgctgttt gtagggacga tctggcagat
    16381 gggggggcag gacatatggc aaagaccgtg caggcaatca aaggactgtc tcctaaaata
    16441 atagtcgaac cactaattcc agattataat ggggacaaag aatcattaaa aaaaataacg
    16501 aactccgaac cacaagtcat atctcataat atagaaactg tagaacgact aacgcctaaa
    16561 gtaagagatg ggagggcatc atacgaacaa agtctttttg tcctaaaaac aataaaaaac
    16621 attgattcaa aaatttatac aaaatcttca atgatgcttg gtcttgggga aaacaaggaa
    16681 gaagttcttc aaacattaaa ggacttaaga gctgttgggg tagatgtggt gactatgggt
    16741 cagtaccttc agcctacaat taagcatctg cccgttgtgg agtacgtgcc accaaaaaaa
    16801 ttcgacgaat acaaaaaaga agctgaacat atgggattcg catacgtagt ggcaggcccg
    16861 tttgtcagaa gctcatacaa ggcatccgaa ccgtttatca aaaacataat tcaactaaaa
    16921 acaagatagg atctaattgc gatgattggc tacgccgggg tgtaaccctt tgcctttgag
    16981 gcaactgacc gtgcttgggc atttgccttt tgtaatatgg gctcttttgt ctcatcggtg
    17041 acatatcctg cctcaactgc aacagagcgt gagccttgag ctactttggc aagtatttgc
    17101 ttgatgttat ctttcgtgat gtaggctgcc tcaagtgata gtgagagtgc ctcttggtgg
    17161 gctctggcaa ataattctcg gtattttgcc aaatcaatga ttagctcttc ttttttgtat
    17221 tgaatgcctt cttctagtgc ggcctcaagc aggatgcctg cctcaattgt ctttatgtcg
    17281 agtttctgaa gcaaggttgc tagcttttct gatacagcgc cacctttctt tactggagtt
    17341 gtgtcctttg taatccaaat tgttccttga tcaatttttg ttggtacgcc agcttcctta
    17401 aactcagtca gcattggtcc tggagcaata cctgtgttct ttgcaggaat catgacatca
    17461 atacttgcga tatcgcctcc gcgggcatac atcatcactt tattttttcc aagcaaaaca
    17521 ttcagcttaa atggagacat attcgtaaac atcaaaatac attggccagt aagggattct
    17581 accatttttg aaattccagg aatgtttaga gctgaaaatg ccttctttac tactttgtcc
    17641 tttatactta ctacctcaac ttcaccgagg aatttttttc ttaagggtaa tagttgtgat
    17701 gatctaactt tttccattct aactagagca gtaaccttgt atttttttgg aagctcttgc
    17761 atctgttgat acatctgcat ttttttctgt ggataagttg ttctgttttc tttcatacta
    17821 ttttcttgcc tgttgtaatt gtttgattgg ttttcccatt gatgtcttga ttattatttt
    17881 tctaatgttt ctttcgccac ttgggagctt tttctcaatt gcgtttagga tggcgtgtgc
    17941 gtttgcagcc aagtcttcat cagacattga ctcatcgcca attttgcatg atagtgaaag
    18001 agagttcttt acctttactc tgattgatga tctaaatctg tccaaaaacg agtctattgg
    18061 cgcattaaat ggcactggca caggcatctt acctcttgga ccaagaagct gacctaagac
    18121 cttacccaca gttgtcataa gctgagtgtc tgccaaaaag aaatcatagt tgttgatgaa
    18181 cttgcgtgct ttgcgcttgt ctgctgcaag cgagtttatc tcatcgccgc taattacacg
    18241 atcggcattt gcgttttttg cctttaatcc catgtctccg ccagccatga tgcaaaccgt
    18301 agttggggtg ctaagcttct ttggcaactg gatggtttca ttcatcgcaa atcctttttt
    18361 tacatcaata tctttgagaa tcacatacat ctctacggac tgcttgaact tgcgttcctt
    18421 gtcggcagcc tttgcctcct ttatcatttt aactaaatct gcttcactga tcaacgaagt
    18481 ttttccaaga acgcctattt aaaatcgttt gttgcttgca aaataaattt tatcaaaaat
    18541 tgtaaaattt tgcaattagg gcgtggtgct ttgtattata tgcaaattct tgtgcctaaa
    18601 cctacattta ttaagttcaa atccttcatt tcgaagacaa tgcataaact agataatttg
    18661 gacatgaaac ttctctacga attaacaaaa gatggaagca tttctgtccc gaatctctca
    18721 aagaagcttg gaatcaacgc atctgtccta tacagcagga tcaagagact tacaaaaaag
    18781 aagctaatta aaaaatttac aatcgttata gatgaggccc aactaggaat tggcgtcaag
    18841 gctacgatcg gaattaacag agatccaaaa caaaaggacg ccatccacag agaattgttg
    18901 caaacaccag aaattgcttc aatttccgag gttacgggaa gattcgacat aatggttggc
    18961 gttcatgcag aaaaccttga aagattacac aacatagtga ttgacaaaat tggcaaaata
    19021 gaaggaattc agagtacaga aacctttgta gagctgcaaa aaaccgacaa agagccctct
    19081 tatctggttt ctaaataatt cactttagtt tatcgtccca ttttcctgca ttgacttctg
    19141 cagtgaattc ctttgggctc ttgccttcaa ttttaattcc caaactcaca caagtcccag
    19201 caacctcttt tgcaactgat tttacggagc tggcatacga tgattctagt tttgccttgg
    19261 ccactttgac tagtgagtcg acattaatgt cgccaaccca cgttgatcca ctggttcccg
    19321 agcctttttg aatgcctgcc tcttttagca acaaggctgc tgcggatgga attccaacta
    19381 ttacttccca tttcttcgtc tttatgttaa cagaaactgt tactggaacc ttcattccct
    19441 caaagtcgga ggttttttcg tttatggtgt tgattatctc cattatgttt acgcctagtg
    19501 gtcctaatgc tggaccaagc ggtggacctg cagatgcttg tcctcctgtc actagagcag
    19561 aaatggtttg ggtatcggac atgttgtcac tttttggcgt gaagatttaa tccttactag
    19621 gaagcgctag atattttgag atagtttgaa tctacggtga ctggcaactg ataaggcgca
    19681 tccagcagga ttaccgttgc ctcttgttta tcgttgtcaa ctcgagttac ggttgccttc
    19741 attcctttga atggaccgcc aataatttca actatattat caattgcaat ttgtggggct
    19801 gaggtctttg ttacaagata tccttctatg tctttgaatt ccaattcgcc ccttaactgt
    19861 cctcgtatgt gtcgtattcc ttggagtgca tcaaatgcgg catttgcgtc ctttgcctca
    19921 attacaatgt atcctttgag attatcaact atcagaacgg aaagaatgtt aattttgttg
    19981 gtctttatct tgttttgtag caaattcatc actacttttt cttgacctcc agtggtccta
    20041 actgcaaaaa gatgcgattt aatttcttga gacaattcca tctatctgaa taccgaaaat
    20101 acaaactgaa ttataaatcc aatcgtccca atcgaggcga ctcccaaaac aaccaatctt
    20161 agatgttgca tatagtcgtc tttgtcagat tttctggcaa gcttcattgt atttgccatg
    20221 ttcttgaggg tgactttcag atccatggtt ggggattaat aaggtgtctt tatatctctt
    20281 atttagtgga actgctagtc gcatatcaaa aagatcctgc cggtcataac atggccaagt
    20341 tcatctcaaa aaacatgaaa aatgacggcg atgttttcag aggcaaaaat tttgacttgg
    20401 tgataattcc gtccccagca atttctgctg actggcttga ggaaaaatac cactatgacg
    20461 gatttgtttt cttatcaaaa catgcagcgc agtcaggcga tcttgcactg acgtgtcata
    20521 gtacggggaa tttctccgat gcgcaatttg gaggaaacaa aagacagatt gctgtacctc
    20581 acccacacct tcaaaagtca tacctgcagg cactttggca aaacagaaaa aactttgagg
    20641 aatttcaaat cacgattgag gcgactcacc acggtccgac ggccctctca aaaccagcct
    20701 tgtttatcga agtgggaaca actgaaaaac aatggaatga cgaatcgctt tgtaattctg
    20761 ttgcaaaact ggttttgcag gttttatccg aaccgccaaa ggctaatcca gttgcaatct
    20821 gttttggcgg cacgcactat ccagacaaat ttacaaaaga actgattaaa ggaaaacacg
    20881 cacttggcac agtagtccca aaacacgcct tggagtttgt agacgaggag atgttttcac
    20941 acatattgaa ccaaaacagg caggcagaaa cggcactact tgattggacg gggcttggaa
    21001 aatacaaaca aaaagtattt gatttgctaa aaacaacaga tctcaaagtg ataaagcttt
    21061 gagcaggcta gaacacaggg tttatcaaaa attactaaag gtgccaaagg gcaaagtcac
    21121 cacatatggg gagcttgcaa acgcagtggg gttagaaaat ggacaacgaa tcatagggca
    21181 aatcatgaac aaaaacccat ttcctgtcat cataccatgt catcgtgtag taaaatcgga
    21241 tggaaaaatt ggcggctatt attacggtga aaaagtaaaa acaaaaatgc tttctgatga
    21301 aggcgtttca gtcaaaaacg gcaaaatcca agactgggaa aaaactgtct ttaggttcta
    21361 agtcttttgc tttgctttga cttcatcaat taggcttctt aggtgagcga tatttctcac
    21421 ggtgttgccg cctattttgc gatatagctc ccaaaactgg ggatttgtga tttcttttct
    21481 atcttttgag accttgagtc tgtggcgcaa ggatcgaacc tttgctacat aaactcgttt
    21541 tttgccaaca cgtgcacctt ttctaccctt ctttgaacca gttttagttc cacgttttcc
    21601 tttttgtatt tttttgaact ttgctcttcc acgtgatgtg cctttggcag ctttgattgt
    21661 aattatccca gcagaaatca ggcttcgtat gttgtctcta gtgattgcat ctgcgatatc
    21721 atctgaacta tctgaatcaa actttattct acttagtcca acaccggcta ctcttgaaac
    21781 taggcgcttt tttgccttaa gatttactac cacttgtctt caccctagca ttaaaaactt
    21841 tgaatttctt ttcgattgct tttgtaacta tgtctaatct tttctttttt ccaacactgt
    21901 gggcaattct taccccatca ttttttggat caagttttgc taaatcatta acattgtaaa
    21961 caagattgtc tgtaaatcct gatggatgca aaccgcgcac cagtttagga ccaccgtaac
    22021 caatcttcac taatccgggc ctgcctctga acttttgtct tctctgatgg ttgtcaatac
    22081 cctttggctt tctccaagcc aagccgatcc ttctgtatcg ccaact
//
LOCUS       DASWKH010000002         5053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_9052, whole genome shotgun sequence.
ACCESSION   DASWKH010000002 DASWKH010000000
VERSION     DASWKH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9052"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1289)
                     /gene="ychF"
                     /locus_tag="VGA92_00130"
     CDS             complement(69..1289)
                     /gene="ychF"
                     /locus_tag="VGA92_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF-related putative GTPase"
                     /protein_id="HEX9844851.1"
                     /translation="MPIKIGIIGKTNTGKTTFFNSATLSSSEISTYPFTTKKPQSATT
                     NAITICVHPEFNVHDNPKNSKCVDGWRYIPIELIDLPGLIKDAWQGKGLGNQFLSVAA
                     QSDALLHIVDVSGSVDSSGKITEVGSGDPISDFADIEEELIMWYLKILEGNREKVSKT
                     IHSGTDKIIAITDLYRGIGVHNSHVKEALHIAGLEDKDFDEIDFQESKKFASILRKIS
                     KPTLIVANKIDIDGADKNFARLRERYIDTIVLPASADSELSLRRAEQKGLIKYSPGSE
                     QFDIIKPDELSQKQKNALDFIKKEIMGEYMRTGVQFAINVTVFKLLKMNAIYPVADEE
                     NLSDKKGNVLPDLILLKDGATVADLAKEIHSDLTRGLLYAKDIRYNLRLPTTYQLRDR
                     DVISLVSASTKKSH"
     gene            complement(1325..1687)
                     /locus_tag="VGA92_00135"
     CDS             complement(1325..1687)
                     /locus_tag="VGA92_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844852.1"
                     /translation="MTTFDDFYKSLKDLVDSYTKKDLMIKMNADLQSNIVRIFGEGMS
                     SLARAKSGLDDVSELAYTTAEHHPYWNLLYHASQISKTTLDKWDSDLTKEELDEISWS
                     IDELKNTCEKLKEKLGQK"
     gene            complement(1687..2097)
                     /locus_tag="VGA92_00140"
     CDS             complement(1687..2097)
                     /locus_tag="VGA92_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9844853.1"
                     /translation="MEQDSHTDEQIRNILSLKNVAVVGMSKNEEKAAHYVPKYLIQQG
                     FNVMPVNPTADKILDRKCCPSLQDVEQQIDIVDVFRPSDQVLPVIEEAIKKKPKVIWL
                     QEGIHNPEAEALAKKAGIDVVFNRCMLAEHQRLF"
     gene            2170..2706
                     /locus_tag="VGA92_00145"
     CDS             2170..2706
                     /locus_tag="VGA92_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9844854.1"
                     /translation="MEEEPKDIIVLGAIKKGAKKFAKIRDKTGIDPEELNKILEKLEE
                     RGFILVEKKKGFLGGEKTELHVTDKGSNELEQRIHEMEQKWDKMVQLYKSGDKKQLQE
                     YMDQNRMDFPMMMFFGMMNMMMFSMMFSMMGASMYDYVPQDQVPPGAENQPADSGDAG
                     GGAGGDTGDGGFDIDIGF"
     gene            2803..3807
                     /locus_tag="VGA92_00150"
     CDS             2803..3807
                     /locus_tag="VGA92_00150"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9844855.1"
                     /translation="MLYTSYDNLGLVKVLQFLKAHQLEYLSGEDLSEVLKISRVAVWK
                     HIKKIQTLGYKIESKQNLGYRLSRTTDLMLPWEITDGLKTKQIGKKIYYFDSVDSTQN
                     FASTISKNSDEAGSVVIAETQSSGKGRLGRAWISPKGGIWLSVILHPTFDVSKITLIP
                     LAAGVALSNAILKTLKIETELKWPNDITLNGKKVAGMIIDATVESSSIESLVLGIGIN
                     FKINPKEVEKKIKSKENFYGIATLLKINEKKNPSKLVQSFLEELEKILMLLNEDKTNS
                     IISLWTKKSSTMGRMVSISTQNGKIIGKAVKLDRDGSLIVKQNSKQLKVTVGDVTYKK
                     "
     gene            complement(3808..4188)
                     /locus_tag="VGA92_00155"
     CDS             complement(3808..4188)
                     /locus_tag="VGA92_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844856.1"
                     /translation="MSGLGNNLNDLRRRVQKAQEELAQIGAPEPPHPEVINTTNLLRT
                     NEYLAKTDEKKTELLSAYEGYAKQLEQIVASLLSIRTDLNAVKAQTSRTKRKKPQKKQ
                     RVKKRKKTKRRKVKKHKKTKKRKK"
     gene            complement(4290..4580)
                     /locus_tag="VGA92_00160"
     CDS             complement(4290..4580)
                     /locus_tag="VGA92_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844857.1"
                     /translation="MMGFKGVYCEGLKSKQLDSNSASRDLYNKKRADIVNELVDPICV
                     ISNANEILSRCMGKYVDDETRSYFEMIDRAIIKTKMLVEELRNESDQDTQSS"
     gene            complement(4577..>5053)
                     /locus_tag="VGA92_00165"
     CDS             complement(4577..>5053)
                     /locus_tag="VGA92_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844858.1"
                     /translation="SFVIIQGRSNIYSRVSKLLQKASGIVYIVAPPEDLMRMYHTPIP
                     ERIEAIKENGADVRILTEVSGGKELSFIHRLNPTEIRLGKLPSKSRMIVEESKQIIMS
                     GALNASMDLNDESDSVMQTNSMEMVSNMFSLCTLLWNKSKRVEIVVEAKAKKVRGQ"
BASE COUNT         1399 a         1048 c         1042 g         1564 t
ORIGIN      
        1 attatacgtg aatcgttgtt taacatgaag tggttcgaat ccgtccagat tcagcaccta
       61 ttttggatct agtgcgactt ttttgtcgat gcgctcacta gtgagatgac gtctcgatca
      121 cgcagctggt acgtggtagg aaggcgcaaa ttataccgga tgtcttttgc ataaagcagg
      181 cctcttgtca gatcggaatg aatttctttt gccaagtcgg ctaccgtcgc tccgtctttt
      241 aagagtatga gatcgggcag gacatttccc tttttgtctg agagattttc ttcgtccgca
      301 actggataaa tcgcattcat tttgagcagc ttgaaaactg tgacattgat tgcaaactgc
      361 acgccggttc tcatgtactc acccatgatc tcctttttga taaagtccag tgcgtttttt
      421 tgtttttgac taagctcgtc gggcttgata atatcaaact gctcagagcc cggggaatat
      481 tttatgagcc ccttttgctc tgcgcgcctc agcgatagct cgctgtctgc acttgccgga
      541 agcacgattg tgtcgatgta tctctcccgt agtcgcgcaa aattcttgtc tgcgccatct
      601 atgtctattt tgtttgccac aatcagagtc ggcttggaaa tcttgcgcaa tatgcttgca
      661 aattttttgc tttcctgaaa gtcaatttca tcaaaatcct tgtcctcaag gcccgctatg
      721 tgcagtgcct cctttacgtg ggagttgtgc acgcctattc cacggtacaa atccgtaatt
      781 gcaattattt tgtctgttcc agagtgaatt gtctttgaga ccttctctcg gttgccctca
      841 agtattttga gataccacat tatcaattcc tcttcaatat cagcaaaatc agagattggg
      901 tcacctgagc ctacctcagt tatcttacca gacgagtcaa cacttcctga gacatcaact
      961 atatgcagca acgcatcaga ctgggcggca acggaaagga actggttgcc aaggcccttg
     1021 ccctgccatg catctttgat taatcccggt aggtcgatta gctcaattgg aatgtaacgc
     1081 cagccgtcta cgcatttaga gttttttgga ttatcatgca cgttgaactc aggatgtaca
     1141 cagattgtta ttgcgttggt agtagcagat tggggttttt ttgtagtaaa aggataagtc
     1201 gaaatttccg atgacgagag tgtcgcggaa ttaaaaaaag ttgttttgcc ggtgtttgtc
     1261 ttgccgataa ttcctatctt tattggcaca tgtagaattg cgtttttagc tatttatctg
     1321 tgccttattt ttgtcctagt ttttctttga gcttctcaca tgtgtttttt agctcatcta
     1381 ttgaccaaga tatttcatcc agctcttctt ttgtcaaatc agaatcccat ttgtccaaag
     1441 ttgtctttga tatttgtgat gcgtgataca gcaggttcca gtacgggtga tgttctgccg
     1501 ttgtgtatgc cagctcagat acatcatcta gtccgctttt tgccctagct agtgaactca
     1561 tgccttctcc aaaaattcta actatgtttg attgcaagtc tgcattcatt tttatcataa
     1621 gatctttttt tgtgtaagaa tccaccagat cctttagcga tttgtaaaaa tcatcaaatg
     1681 tagtcattag aacaaccgct gatgttcagc aagcatacat ctgttaaaaa ccacatctat
     1741 tcccgccttc tttgcaagtg cttctgcttc cgggttatgg attccctctt ggagccaaat
     1801 cacctttggc tttttcttaa ttgcttcttc tattactggt agcacttggt cagatggcct
     1861 aaaaacgtca actatgtcga tttgctgctc aacgtcttgc aggctagggc aacactttct
     1921 atccaaaatt ttgtccgcag ttggattaac aggcattacg ttgaagcctt gctgtatgag
     1981 atattttggg acatagtgcg ctgccttttc ctcgtttttt gacattccca ccactgccac
     2041 gttctttagc gatagaatat ttcgtatttg ctcatccgta tgggagtctt gttccacaac
     2101 agatagtaca aaaaagagaa ttaaaattta tgtggaaaac gctttataca caaaattcga
     2161 aattatgtca tggaagagga gccaaaggac attatcgttt taggtgccat taaaaaaggc
     2221 gccaagaaat ttgccaagat aagggacaag actggcatcg acccagagga gctaaacaag
     2281 attttagaaa aactcgagga acggggattt atcctggtgg aaaagaaaaa ggggttcttg
     2341 gggggagaaa aaacagaact ccatgtaacc gataagggat caaatgagct agaacaaaga
     2401 atccatgaga tggagcaaaa atgggacaaa atggtccaac tgtacaagtc aggtgataaa
     2461 aaacagcttc aggaatacat ggaccaaaac agaatggatt tcccaatgat gatgtttttt
     2521 ggaatgatga acatgatgat gttttcaatg atgttctcaa tgatgggggc atcaatgtat
     2581 gattatgtcc cacaagacca agttccaccg ggggctgaaa accagcctgc cgattcagga
     2641 gatgccggcg gaggcgctgg aggcgacaca ggtgatggtg gattcgacat tgatattggg
     2701 ttttaaaaat tcaattcaag tattacatgc ccctacacca catttgattc gaatctgagt
     2761 aaatttagaa attttaaatg aatcatatta tgaggcaaga tattgctgta cacgtcttat
     2821 gataaccttg gcctagtcaa ggttctgcag tttttgaagg cacaccagct tgaatatcta
     2881 tcaggtgaag acctaagcga ggtactaaaa atcagccgag tcgcagtctg gaagcacatc
     2941 aaaaaaatcc agacgctcgg ctacaagata gaatcaaaac aaaaccttgg atacaggctc
     3001 agccgcacaa ctgatttgat gctgccatgg gaaataacag acggattaaa gacaaaacaa
     3061 attgggaaaa aaatctatta ttttgattct gtcgattcaa cacaaaactt tgcatcaacc
     3121 atttctaaaa attctgatga ggctggctct gtggtaatag cagaaaccca gagttcagga
     3181 aaaggaagac tgggtcgcgc ttggatttca ccaaagggcg gcatctggtt gtcagtcata
     3241 ctgcacccga catttgatgt atcaaaaatc acactaatac cacttgcagc aggtgttgca
     3301 ctatctaatg caatcctcaa aacactcaag atagaaactg agctaaaatg gccaaacgac
     3361 attacgttaa acggcaaaaa ggttgccgga atgataattg atgcaacggt agaatcaagc
     3421 agtattgaaa gtctcgttct tggaattggg ataaacttta agataaatcc aaaagaagtt
     3481 gagaaaaaaa tcaaatcaaa agaaaacttt tacggcattg ccacgctact aaaaataaat
     3541 gaaaagaaaa atccatctaa gcttgtacaa tcgtttttag aagagcttga aaaaattcta
     3601 atgctcctaa acgaagacaa gacaaattcc ataatcagcc tatggacgaa aaaatcttcc
     3661 acaatgggca gaatggtttc catatccact caaaatggca aaataattgg aaaggcagtc
     3721 aagctggaca gagatggcag tctaatagta aagcagaatt caaaacaact caaggtcaca
     3781 gtaggcgatg taacctacaa aaaatagcta cttttttcgc tttttggtct ttttgtgttt
     3841 tttgaccttt cgcctcttgg tttttttgcg ttttttcacc ctttgctttt tttgcggttt
     3901 tttgcgtttt gttctagatg tctgggcctt tactgcatta aggtctgtcc gaattgaaag
     3961 taatgacgct acgatctgct ccaattgctt tgcatatccc tcatatgcgg agagcaactc
     4021 ggtcttcttt tcgtctgttt ttgccaggta ttcatttgta cgcagcaaat tggtagtatt
     4081 gatgacttcg ggatgcggcg gttcaggtgc accaatctgt gcaagttctt cttgggcttt
     4141 ttgtactcgt cgcctcagat cattcaaatt atttccaaga ccagacatac acgattaaat
     4201 aaaataattc gttataatca tttttgtggt tttgcgcatc aaacagatcg aggcaaatgt
     4261 aaatagtcgt ttaatgccgt caggtgtttt tatgacgact gtgtgtcttg gtcgctttcg
     4321 tttcgaagtt cttctactag catttttgtc tttatgatgg ccctatctat catctcaaag
     4381 tatgatctcg tttcatcatc tacgtattta cccatacatc ttgacagtat ttcgtttgcg
     4441 tttgagatga cgcagattgg atccacaagt tcgtttacta tgtcagcccg ttttttatta
     4501 tacaggtcac ggcttgcaga atttgaatca agctgttttg atttgagccc ctcacaatag
     4561 acccccttga agcccatcat tggcccctca ccttcttggc ttttgcttca accacaatct
     4621 caactctttt tgatttgttc cacaaaagtg tgcaaagact gaacatgttg cttaccatct
     4681 ccatagagtt tgtttgcatt acggaatcac tttcgtcgtt gagatccatt gatgcgttca
     4741 gcgcgccaga catgataatc tgcttgcttt cctccacaat cattctgctc tttgatggaa
     4801 gcttgccaag tcttatctca gttgggttca gcctgtgaat aaatgatagt tctttgccgc
     4861 cagacacttc agtcagaata cggacatcag cgccgttttc ttttattgcc tcaattcttt
     4921 ctgggattgg ggtgtgatac attcgcatca gatcctctgg cggcgcaaca atgtaaacaa
     4981 tacctgaggc cttttggagc agtttactga cccgagaata aatgtttgag cggccctgaa
     5041 taattacaaa aga
//
LOCUS       DASWKH010000003         6354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1118633, whole genome shotgun sequence.
ACCESSION   DASWKH010000003 DASWKH010000000
VERSION     DASWKH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118633"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VGA92_00170"
     CDS             complement(<1..545)
                     /locus_tag="VGA92_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9844859.1"
                     /translation="MDEILRVENLKKYFTKKNWLGKQSSLVRAVDGVSFSVRRGESFV
                     LAGESGSGKSTIAKLILRAITPDDGKIIFDGVEITDKKEDLKKIRMQCQMVYQDPYDS
                     VNPQMKIKDIISEPIEIHRIGDSKQRLELVKEVLREVRLEPVDEILEKYPHQLSGGQR
                     QRVVLARALVLKPKLIIADEPV"
     gene            complement(582..2027)
                     /gene="proS"
                     /locus_tag="VGA92_00175"
     CDS             complement(582..2027)
                     /gene="proS"
                     /locus_tag="VGA92_00175"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9844860.1"
                     /translation="MSKESVGITVSKKDDFSEWYTQVVLKAELADYAPVKGLIVLRPD
                     GYAIWESIKESLDKKFKKTGHKNGFLPVLIPESLLGKEKDHFAGFNPEVFWVTHSGDS
                     EIGDRLALRPTSETLAYTLYSKWVQSWRDLPLKINFWNTALRAEIKATKPFLRTSEFL
                     WQEGHTVHTTKEEAESEVLEILDIYKKTVEEELSIPVIVGKKSDKEKFVGAVYTTTME
                     AMMPDGKALQMGTSHFLGQNFSKPFDVKFLDKNNVETYAWQTSWGVSWRLIGATIMVH
                     GDDKGLVLPPKIAPIQVVIVPIYYSDKDIQRVHSKADDVEKILQDKGIRVHVDRRDEL
                     TPGFKYNEWELKGIPLRIEIGPKDLDKNKVTVARRHTKQKSDLPFDQIESQIDGILQQ
                     IQVEMFETAKKSLNERIVKESDYSGFKSALEEGCFIDAPWCGKTSCEEKIKEETMADI
                     RVIPFESKNTNGKCIYCKEQSVANVIFGRAY"
     gene            2144..3994
                     /locus_tag="VGA92_00180"
     CDS             2144..3994
                     /locus_tag="VGA92_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM domain-containing protein"
                     /protein_id="HEX9844861.1"
                     /translation="MKTKVIPAVIAALFFAVGFSSASADSSEKLDFASYLEETLGHFH
                     ALELNLDDDNAELAIVHATHPIAELYDLMKPELQEHDPEFDARIQDTLTNLRSNTVDV
                     TREDAQAALDEAREIIEEARMLIVGEELSNDPSFKMSLIKTLLETSIVEYAEAVENGA
                     IHEMAEFQDGSAFVWKSQQIFDEIKADLPEHEVEEIDEFYADLWHAYDQRADPSTVET
                     LASGVIHEIEEIAGEESDEKLAFAGYLEETLGHFWAIELNLDEQNAALALVHATHPIA
                     ELYDLMKPVLTEADAEFDAKVKQTLDALGEKTGSDVSREDAQSAIDEARDIIEEARMI
                     VVGEELSNDSTFQLGLIKGLLETSAAECAEAVESGAIHEMAEFQDGSAFVWRSQQIFD
                     EIKADLPEHEVEEIDEFYADLWDAYDKRADPTLVRTLVDGIIHEIEGIAGEESDDGGL
                     HAYFENIEHLLAETSEEYAGGNTDLALSLATKAYLDNYEFLEGPISEHDDELMVEIEV
                     MMREELRNMIKNGAPLDDIDSQIDTILEKLEDAEVLVLGSMEHEEEHEEENTYADLTP
                     LKQVDVGIEPEDVVCASGMELLIKNSNGSPACVKPETAERLIQLGWGTRQ"
     gene            complement(4017..4304)
                     /locus_tag="VGA92_00185"
     CDS             complement(4017..4304)
                     /locus_tag="VGA92_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844862.1"
                     /translation="MGELEHKYEVEVKGAEKPQKLPDNVKEELKASGMMDGKGKIKLK
                     GVSPKMLKRMKQEYVDCPVLKQDVGFVQCFVCPNFQSRVMGKVLCKGNALK"
     gene            4408..5058
                     /gene="serB"
                     /locus_tag="VGA92_00190"
     CDS             4408..5058
                     /gene="serB"
                     /locus_tag="VGA92_00190"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX9844863.1"
                     /translation="MLAIFDVEGVLFDAEYLPILAEKVNKEKEIWEITKKGIQGVINW
                     EDGLRTRIDALRGLSYDVCKQVADELPIMTGAKETCRVLKSAGWKLLAVSGGFTIMTD
                     RLKEELNLDYVFSNELLFKDGKLDGVKINVTSDKAKSAKIKISEWTEKKENIVVTVDG
                     ANDMTLFDICGLGVAFRAQDVVKEKANVTIEEKNLTNLIDVINKHYDLKLQLQTVA"
     gene            complement(5093..5269)
                     /locus_tag="VGA92_00195"
     CDS             complement(5093..5269)
                     /locus_tag="VGA92_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844864.1"
                     /translation="MMQVKLKHEQLEKIHGEIYGLRKDDSPICIQLEKLDRYIQERGG
                     KGFKPDTFDTFEYI"
     gene            5427..6161
                     /gene="cofE"
                     /locus_tag="VGA92_00200"
     CDS             5427..6161
                     /gene="cofE"
                     /locus_tag="VGA92_00200"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX9844865.1"
                     /translation="MPVEIIPVVLKKEIQKGDDIVQLFLSNFKDLSDGDIVVVAQKIV
                     SKQEGRVVDLAGVIPSLLAVGIAAEYEKDPKLIEVILSESKRIVRMHNGIIITETKHG
                     FVCANAGVDESNLPLGFASTLPENPDKAASEFQQKVASKTHKKIAVLIADTFGRTFRE
                     GQTNCAIGIAGIKGIVDYAGKKDVFNRILRVTAIAHADEICGAAELVMGKTKDCPIAV
                     VRNFEFESSDGGVNSLIRSKQTDLFR"
BASE COUNT         1910 a         1296 c         1316 g         1832 t
ORIGIN      
        1 acaggttcgt ctgcgattat cagttttggt tttaagacaa gtgcccttgc taaaaccact
       61 ctttgtctct gacccccaga taactggtgg ggatattttt ctaatatttc gtctactggt
      121 tccagtctta cttcgcgcaa aacctctttt actagttcta atctctgttt tgaatcgcca
      181 attcggtgga tttcgattgg ttctgaaatg atgtccttta ttttcatttg aggattgact
      241 gagtcatagg ggtcttgata caccatttgg cactgcattc gaattttttt tagatcctct
      301 tttttgtctg tgatctcaac accgtcaaaa ataattttcc catcgtctgg cgtgattgct
      361 cttagaatca attttgctat tgttgatttg ccagagcctg attctccggc aagaacaaaa
      421 ctctctcccc gcctcacaga aaaagatact ccgtctactg ccctaactag cgatgattgc
      481 ttgccaagcc agtttttctt tgtaaagtat ttttttaaat tctctacgcg gagtatctca
      541 tccaacatct gaaattactc gtaatactat atcaagaatt gttagtaggc tcgaccaaaa
      601 atcacgtttg ctacactctg ctctttacag taaatgcact tgccgttcgt atttttactc
      661 tcaaatggaa taacgcgaat atcggccatt gtctcttcct ttattttttc ttcacagctt
      721 gtctttccgc accacggcgc atcaataaag cagccctcct ctagcgcaga tttaaagcca
      781 gaataatccg attcctttac aattcgttca ttaagcgatt tttttgccgt ctcaaacatc
      841 tcgacttgaa tttgctgcaa aattccatca atttgtgact cgatttgatc aaacggcagg
      901 tcggattttt gcttggtgtg acgtcttgca acagtcacct tatttttgtc taggtccttt
      961 gggccaatct ctattcggag ggggattccc tttaactccc actcgttgta cttgaagcca
     1021 ggtgtgagct catctcgtct gtcaacatgg acacgaattc ccttgtcttg caaaatcttt
     1081 tccacatcgt ctgcctttga atgaactctt tggatgtctt tatctgaata ataaattggg
     1141 acaattacaa cttggattgg cgcaatcttg ggaggcaata ccaaaccttt gtcgtcgcca
     1201 tggaccatta tcgtggcgcc aattaatcgc catgatactc cccaagacgt ctgccatgca
     1261 tatgtctcaa cattattttt atcaagaaac tttacatcaa atggttttga aaagttctgt
     1321 ccaagaaaat gcgatgtccc catctgcagg gcctttccgt caggcatcat ggcctccata
     1381 gtggtagtat atacagctcc aacaaatttt tccttgtcac tttttttgcc aacaatcaca
     1441 ggaattgaaa gctcttcttc tactgttttt ttgtaaatgt ctaaaatttc taatacttcg
     1501 ctttctgcct cctctttagt tgtgtgaacg gtgtgccctt cctgccacaa aaactccgaa
     1561 gttctaagaa atggctttgt tgcctttatt tctgcacgta gtgccgtatt ccaaaagttg
     1621 atctttaatg gcaagtcgcg ccagctttgt acccatttgg agtatagtgt gtatgcaagg
     1681 gtttcagatg ttgggcgtaa cgccagtctg tcgccaattt cggaatctcc agaatgtgtg
     1741 acccaaaaga cctctgggtt gaatccggca aaatgatctt tttcttttcc taaaagtgat
     1801 tctggaatta aaacgggcaa aaagccgttt ttatgtcctg tctttttgaa tttcttatcc
     1861 agcgattctt tgattgattc ccaaattgca tatccatcag gcctaagaac aatgagtcct
     1921 tttactggag catagtctgc cagctctgct tttaggacaa cctgggtgta ccattcacta
     1981 aagtcgtctt tttttgagac tgtgattcca acactttcct tactcaatct gatatcctgc
     2041 cagaattttg cctaatgagc ttttcttagt tgctccaatt cgttattcta agaatatgta
     2101 ataatctaat ccgtttaaat gatatagttc gatccgcatt cacatgaaga caaaagtaat
     2161 ccctgcagtt attgcagcat tattttttgc agtagggttt tcttcagctt ctgcagactc
     2221 gagcgaaaaa ctcgactttg catcatatct agaagaaaca ctagggcatt ttcatgcact
     2281 tgagcttaac ttggatgatg acaatgcaga acttgcaata gttcatgcaa cacatccaat
     2341 agcagagctg tatgatctaa tgaaacccga gcttcaagaa catgatccag agtttgatgc
     2401 gagaatacag gacacgctca caaacctacg ctcaaacacg gtagatgtca caagggaaga
     2461 tgcacaagca gcacttgatg aagcaagaga aatcatcgaa gaggcaagaa tgttaattgt
     2521 tggtgaagaa ttgagcaacg atccatcctt taagatgtcc ttgattaaga cgctacttga
     2581 aacatctatt gtggaatatg ccgaagcagt agagaatggc gcaatacatg agatggcaga
     2641 attccaagat ggctctgcct ttgtgtggaa gtctcagcaa atctttgacg agattaaagc
     2701 agaccttcca gagcacgagg tagaagaaat cgacgaattc tacgctgatt tgtggcatgc
     2761 atacgatcaa agagcagacc catcaactgt tgaaactctt gcaagtgggg taatacatga
     2821 aatcgaggaa atcgcaggtg aagaatctga tgagaaactt gcgtttgcag gatatctgga
     2881 agaaacacta ggccactttt gggcaataga actaaatcta gatgagcaaa acgcagcact
     2941 tgcgttggtt catgcaacac atccaattgc agaactatac gatctgatga agccagtgtt
     3001 aactgaggca gacgcggaat ttgatgccaa ggttaaacaa acattggatg cattaggtga
     3061 aaaaacaggt tctgatgtat cacgagaaga tgcgcaaagt gcaatagatg aagcaagaga
     3121 tatcatcgaa gaagcaagaa tgattgttgt gggcgaagag ctaagcaacg attctacatt
     3181 ccaactcgga ctgatcaaag gtcttttgga gacatcagca gctgaatgcg cagaagccgt
     3241 agagagcggt gcaatccacg agatggcaga attccaagac ggctctgcct ttgtgtggag
     3301 gtctcagcaa atctttgacg agataaaagc agaccttcca gagcacgagg tggaagaaat
     3361 cgacgaattc tatgccgacc tttgggatgc atatgacaaa agagcagatc caacactagt
     3421 gcgaactctg gtggatggaa taattcatga aatcgaggga atcgcaggag aagaatctga
     3481 tgatgggggt ctacacgcat actttgaaaa cattgagcat cttctagctg aaacaagcga
     3541 agaatacgct ggaggaaata cggaccttgc attaagtctt gcaaccaaag cgtaccttga
     3601 taattacgaa ttccttgaag gtccaatctc agaacacgac gatgaattga tggtagaaat
     3661 tgaggtaatg atgcgtgagg agcttcgtaa catgatcaaa aatggagctc cattagatga
     3721 catagactca cagattgata ccatcttaga aaaactcgaa gatgcagaag tattggttct
     3781 aggctcaatg gaacatgagg aagagcatga agaagaaaat acatacgcag acctcacacc
     3841 actcaaacag gttgatgttg gtattgaacc agaagacgta gtatgcgcat ctggaatgga
     3901 actactcatc aaaaactcaa acggctcacc cgcatgtgtc aaacctgaaa ctgcagaaag
     3961 actaatccaa cttggctggg gtaccagaca ataatctctt tttttattac aaaattttac
     4021 tttagtgcat tgcccttgca gagaactttg cccattaccc tgctctgaaa attaggacac
     4081 acaaagcact ggacaaagcc aacatcctgt tttaggaccg gacagtcgac atattcctgc
     4141 ttcattcgct ttagcatctt tggactgact cctttgagct tgatctttcc tttgccgtcc
     4201 atcatccctg atgcctttag ctcctctttt acattgtctg gaagcttttg tggtttttcg
     4261 gctcccttga cttcaacttc gtatttgtgt tcaagctcgc ccatgttgtg agttttttac
     4321 taccttgatt tattattagc ggttgtcgat ctcaaaataa acttcaactt ttatattgac
     4381 agcgttttgt ccaaaagttg gaaacaattg cttgcaattt tcgatgtgga aggcgttctc
     4441 tttgatgccg aatatctacc aatccttgca gaaaaagtaa ataaagaaaa agaaatttgg
     4501 gaaattacaa aaaaaggtat acagggagtt ataaactggg aagatggact gagaacccga
     4561 attgacgcac taaggggcct aagctatgac gtgtgcaaac aagtggcaga cgagctccca
     4621 attatgactg gtgcaaagga gacgtgcagg gttttaaaat cggcaggatg gaaactactt
     4681 gcagtctctg gtggctttac aataatgact gacagactca aagaggagct gaaccttgac
     4741 tatgtgtttt caaacgagtt gttattcaaa gatggtaaac ttgatggtgt aaaaataaac
     4801 gtgacttcgg ataaggcaaa atctgccaag atcaaaatat cggaatggac ggaaaaaaag
     4861 gaaaacattg ttgttacagt tgatggtgca aacgacatga ctctttttga tatctgcggc
     4921 cttggggttg catttagggc acaagatgta gtaaaagaaa aagcaaatgt cacaattgaa
     4981 gagaaaaatc taacgaacct aatagacgtg ataaacaagc attatgatct taaattacag
     5041 cttcagactg tagcgtaact cagacgcatc aaaagtaacg tgttgactag ttctagatgt
     5101 attcgaaggt gtcaaatgtg tctggcttga agcctttgcc accacgttct tggatgtatc
     5161 tgtcaagctt ttctagttgg atgcaaattg gagaatcgtc ttttcttaac ccgtagattt
     5221 ctccatggat tttttccaac tgctcatgct ttagctttac ttgcatcatg cgttaatact
     5281 ggtggcagct ttatctaaaa atgatcaaaa attattcgtt caaattaatc aaacccgctt
     5341 tgaaacctac atcaagccaa tccatatctg ggatgataaa atccgaacaa ttcaagttaa
     5401 attagaatcc atactttcaa ttttaaatgc cagttgaaat cattccagtc gtactaaaaa
     5461 aggaaataca aaaaggggat gatattgtcc aactcttcct ttctaatttt aaggatctga
     5521 gcgacggaga tattgttgta gtggcacaaa aaatagtttc aaagcaagaa ggacgcgtcg
     5581 ttgatctggc aggcgtaatt ccatcgcttt tagcagttgg aattgcggca gaatatgaaa
     5641 aagatcccaa attaatagag gtgatactct cagaatcaaa acgaattgtt agaatgcaca
     5701 atggaataat tataacggag acaaaacacg ggtttgtctg cgcaaatgca ggagttgatg
     5761 aaagcaacct tcctttggga tttgcgtcaa cgctgccgga aaatcctgac aaagcggcat
     5821 cagagtttca acaaaaagtt gcatctaaga cacacaaaaa aatagcagtg ctcatagctg
     5881 acaccttcgg ccgtacattt cgggaaggcc aaacaaactg tgcaattggc attgctggca
     5941 taaaaggaat tgttgattat gctggaaaaa aggatgtctt taacagaata ttgcgagtaa
     6001 ctgcaattgc ccatgcagac gaaatttgtg gagcagcaga gcttgtcatg ggaaaaacaa
     6061 aggattgccc aattgcagtg gtacggaact ttgaattcga gtcatccgat ggtggcgtca
     6121 atagcctaat tcgctcaaag caaaccgact tgttcaggta acctgcacaa aaaagattat
     6181 aatcattgcc aaaaagattg caattgattt ggactatatc aaaaccgagt tacactgcca
     6241 taactcattt tccaacttta acgtgggtga ggaggaacca ccatacgact gtaacataac
     6301 aattcaagaa caactcgagc atgcaaaggc cttgggcctt gagggggtct ttgt
//
LOCUS       DASWKH010000004        56259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3335600, whole genome shotgun sequence.
ACCESSION   DASWKH010000004 DASWKH010000000
VERSION     DASWKH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 56259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56259
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3335600"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGA92_00205"
     CDS             <1..411
                     /locus_tag="VGA92_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844866.1"
                     /translation="ISEPTKLYSPIVKIKSLPLSLRQNGDEQLEKYELLELEDLASLA
                     KISYGYDEAPFPLFAFPHKDNKWLLGVFMNFNEEGPSYFCHVVLDSEPSRPFLKYAMH
                     NGNMPVFTDNVNEHGYTYIKIIKLREAHPLVDYV"
     gene            404..1147
                     /gene="pyrF"
                     /locus_tag="VGA92_00210"
     CDS             404..1147
                     /gene="pyrF"
                     /locus_tag="VGA92_00210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9844867.1"
                     /translation="MSSFKERIKKLASEKSSLVLANDYENHNTILESKTLGNIKTLNQ
                     YLCAIKFNFHLLLPLSQKQITKINKAAHDSGLQTIADIKLNDIGNTNFVAAQTLWDLG
                     FDAVIVNPMMGPSSLENLVKVAHKNKKGIISLCHMSSPEAKVTYELEVQTQSKKTNLY
                     KLFLEWATKTNADGIIVGATYPQIVKFCKQKTSGKINIYSPGIGIQGGDIQKTIRSGS
                     DYLIVGRTILKSKNPKQTAKSLFDLAGKN"
     gene            complement(1130..1552)
                     /locus_tag="VGA92_00215"
     CDS             complement(1130..1552)
                     /locus_tag="VGA92_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9844868.1"
                     /translation="MSEFDERSAGILLFCEESGKKLFLLLHYPSGHWDFIKGRIEKNE
                     RPKEAAIREAREETGITDIEFIDGFEQKIQYSYQFGGKVKRKEVVFFLAKTNTTTVKL
                     SNEHINYAWLEFDEAFKKTTYQNAKELLKKSKSLVFAS"
     gene            complement(1553..2269)
                     /gene="uppS"
                     /locus_tag="VGA92_00220"
     CDS             complement(1553..2269)
                     /gene="uppS"
                     /locus_tag="VGA92_00220"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX9844869.1"
                     /translation="MRKGNIPNHIAVILDGNRRWAKKILETSQKGHFMGADAVENLLD
                     WCEELDIKIITLYVLSTENLDRTDEELEYLFKLIHQRLEKLYTDARIHKNKMRVKAMG
                     RTELLPDYINDVLQRLDDATKGYNRHYLNIAIAYGGQNELVDAIKKIGTRIKDGSIGV
                     NDINKDVIEANLYTAHLPQPSPDLILRTSGEQRMSGFLLWQAAYSELIFMDILWPEFR
                     KIDLLRAIRIFQKRERRTGK"
     gene            complement(2332..3486)
                     /locus_tag="VGA92_00225"
     CDS             complement(2332..3486)
                     /locus_tag="VGA92_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HEX9844870.1"
                     /translation="MSLKDSNLEKALENQVTSRLLVICVDRDDDVGKKAGIATPVVGR
                     NACIEAAQKLALEDPEDADANSIFFAVKTYEDLARKGYQAEVIVVAGVEERGVQADEK
                     IVREVKAVLQSYSANGAVIVSDGEDDESVIPIIQNVVPIVSVQRVVMRVSRSVEYSYA
                     VFGKYLKMIAYDSKYSKFFLGVPGILLLIGGIGAISGYTEEIFAVLVSILGGAFLIRA
                     FDVDRTLSSWAKPTPASLIRAFTMVAGIIIGLAAVLSGFSAINTNILDPHILDSGIPN
                     ILADTVTLGQFIAGALPFLWMGMGSVFGGILLSHWLKGSLRQITDILRMIVLVSLYPT
                     VHQFTNLLIHDESSFTLIPPFLAGLAITLISATFLFRKYRKKKGGEVLTE"
     gene            complement(3564..4061)
                     /locus_tag="VGA92_00230"
     CDS             complement(3564..4061)
                     /locus_tag="VGA92_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844871.1"
                     /translation="MQSKKGFAITGIILAAITVASFTVWLVPQNTQTKFVVSNAEEEL
                     DALIEQQKTISDSTLEEFDKMFAGQATPDSYISVAEISSSQINSFIIKIIEGEVPQEW
                     QTSYSSYIDFLRAYNSYLRETMVIANKVKENPQADISQDMTALDEYLKQADEFLAISN
                     SARPA"
     gene            4309..4455
                     /locus_tag="VGA92_00235"
     CDS             4309..4455
                     /locus_tag="VGA92_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX9844872.1"
                     /translation="MATAYCVKCRAKREIKDPKVTKLKNGRPAVKGTCPKCGTNVFRI
                     GKLD"
     gene            4518..4940
                     /locus_tag="VGA92_00240"
     CDS             4518..4940
                     /locus_tag="VGA92_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HEX9844873.1"
                     /translation="MAKTDYEKLLKRMQDKISIKKSDSGERFELPTPNVMWEGQRTIL
                     RNFMDYPKILRRDPDKVLQYLAKEFATPAERSGEQAMFVGRRDPHDFVNLFEIYIKDY
                     LECPTCKSPDTRIERENRISFLVCEACGAKSSLKGKYA"
     gene            4982..5236
                     /locus_tag="VGA92_00245"
     CDS             4982..5236
                     /locus_tag="VGA92_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HEX9844874.1"
                     /translation="MLNICDEDVLGKVLKKDHLQINISKSYYGQRLIEKDEAENLMRD
                     STILNLVGKETIEMSINLKIGSRQGVKVIEDVPFLIVFKM"
     gene            5276..5587
                     /gene="eif1A"
                     /locus_tag="VGA92_00250"
     CDS             5276..5587
                     /gene="eif1A"
                     /locus_tag="VGA92_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HEX9844875.1"
                     /translation="MGKRKVLNESELKDIQLPQQGELLGRVIKLLGSDQVLVKCMDGL
                     TRRGRIRGKLKRRIWIRDNDVVILAPWDFKQEDRGDIIWRFTLAQVDWLKNNGHLPKD
                     F"
     gene            5611..6399
                     /locus_tag="VGA92_00255"
     CDS             5611..6399
                     /locus_tag="VGA92_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HEX9844876.1"
                     /translation="MSEDDIEIADDDFDPKFDKIFPKSKHRILQDGFNKDKVVSQVLD
                     RSTMLTMYEMINRKIISYVNGIVKAGKESVLFWAVDSAGKDVALKVYLVTTTSFKNRA
                     KYIIGDPRFSKIKHGTRNLVFLWARKEYRNLKQCEKLNLPVVRPLYVSKNVLAMEFVG
                     KNGIPAPTLHETEIDENDYRQAIQIITRLYKEANLVHGDFSEYNIFKTENGLILFDLG
                     SAVDTLHPNAQNFLERDIKNVSYFFAKRGLTVENPSDVLAKIVS"
     gene            6396..6965
                     /locus_tag="VGA92_00260"
     CDS             6396..6965
                     /locus_tag="VGA92_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX9844877.1"
                     /translation="MSFEKIIRIPVDRVGALIGKSGKVKIQIEKTCFVKLNIDSETGE
                     IEIISDGSISQIQPFKAVEIVTAIGRGFSPQNAMKLIREDNLLYVMDLREFAGKSPQQ
                     IERIKGRIIGEGGRARANMENLTNTSICVYGKTVSIIGEATQIKVVVSAISSISSGSK
                     HGSVYGKLEANRRKQRLSRLQLWEKDDGS"
     gene            6955..8799
                     /locus_tag="VGA92_00265"
     CDS             6955..8799
                     /locus_tag="VGA92_00265"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX9844878.1"
                     /translation="MALKETFNQISPSEFFYRNRDLAGFSNPTRSLYTAVREFVENAL
                     DACDQQGILPGVHLIIKPLDAEKPDPKPYILTVKDNGPGMESKQIPLAFGTVLYGSKF
                     GLKQARGMFGLGATMAILYGQITTNKPVTVASSADGKTEHEYVMMLDIQKNKPVIVKH
                     PAPRQTNKKGLSVSIVLEGDYSKAGSKIRDYVYQTSLITPYATITFDGPKGEKFHYKR
                     IVDTMPPPPTIIKPHPHGVDVETIRRMIVDTHYQIPVLDNTMIEKVRKELGLSKKNLN
                     FDGIMAKAEKKWASLSRPVRVIVALMSFLEMDFEKIMKIRIDDVDLAHKHMTYWDFGE
                     SKSVIVDMPKSNPYYKQLANTVQGESVATFLTKRFQRIGPSTAIKFAEFANLKPEKRI
                     GSLSNEELVQLSDSLQKYEDFLSPDPSCLAPLGEEPLKKGMSKFFEPDFLEVVQRSAS
                     AYSGFPFVIEMGVAYGGKIPSGKITVYRFANRIPLLYDEGSDVVLQVVNETDWSRYKV
                     KNDAPLVIVSHICSTRIPYKTVGKENVADRPEIEKELRLALLFLARKLSAYMSKRGLA
                     EIEKKRTNLYQKYLPLIAQFATDLAGKNKEPDYKKLIKDLNTNVEIKE"
     gene            8780..9883
                     /locus_tag="VGA92_00270"
     CDS             8780..9883
                     /locus_tag="VGA92_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX9844879.1"
                     /translation="MSKSKSKTEKVATAKRDSLLELLKGEGSKIYSELDKGQFPQFYV
                     PSRSVNNIVYDKKLRQYILGKAASLRSSRNMAQLRSFTQLIWLAFFAKKLVNEKKSST
                     LRDIYYSSQAFEIDFEDQPESDNIIIDLEAVLARPREDFHIFPEERSSVFGDLTIEYT
                     VPGYEGRRTNLSDHPDGYLIGPSLSSAELVDTSAELVIAIEKGGLFTRFVEEKVDKKF
                     KAIIIDTAGQAPRSTRHLLKRLHEQMKLPVVILTDGDVYGEHIAMVIKSGSANAAHLR
                     ELTVPDAKWVGVWASDIEKYKLPTIPMTESDIKRIYDLQKDPRYQEGIWKKELEIFLK
                     IKRKAELEAFSKYGLTNITDKYLPEKLQIAKSL"
     gene            complement(9870..10340)
                     /locus_tag="VGA92_00275"
     CDS             complement(9870..10340)
                     /locus_tag="VGA92_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX9844880.1"
                     /translation="MSSYKKTYSNEQSINFIIPIMIILFLGIIYIMSLRSGHGSVSFI
                     LIGIAAATMIYWGFIIKKMTKTDKPKYSVRDTESKNWVYDLIKGDDETIFVAEVPGPD
                     DKIMVRLIEGVLYIRGSGNFSKEVPIEGAANMQIADFKYRNGVLTLRIKKATGS"
     gene            complement(10405..11601)
                     /locus_tag="VGA92_00280"
     CDS             complement(10405..11601)
                     /locus_tag="VGA92_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9844881.1"
                     /translation="MSRKYLNRNEKQSEPTDAFEKTDKEIFLKKDPSSNSTEAKFGSL
                     GDDIRNLDQYIGSLSQHLVDQKARIDAMKKLQNNFEKEIGILNSGGRSIPIQVKKNEF
                     DNVGVEFESEKIIGDITSIVEKQVQNRILVLESQLEEERKNSYEFRKILGENLKEFNR
                     IEKNLKKEKNRLEGEIREKTQKLVQAERLSAIGELASRLAHDLRNPLSVIKATVQLIK
                     HTNESLDDPTRKRVDLIEGSIFRMTHQIDGVLDYVKATPLKKKPSSLNEIIMAALQTL
                     LIPPNITVNVPKAEITFLCDPQKIEIVIANMILNSIQAIGDQKGIINIRTKKYQESVT
                     IRIEDSGNGIVQSIIDKIFDPLFTTKEEGTGLGLASCKNIVEQHKGKISVSTDPTVFS
                     ITLPLY"
     gene            11750..11974
                     /locus_tag="VGA92_00285"
     CDS             11750..11974
                     /locus_tag="VGA92_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844882.1"
                     /translation="MDKSKLNPRPTKGIFLKSSLIGAIITIPSLAAFFISWLITGDKI
                     TSLIIGIIVHFIGMGFSLKISKRLFKIKTP"
     gene            12017..12370
                     /locus_tag="VGA92_00290"
     CDS             12017..12370
                     /locus_tag="VGA92_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844883.1"
                     /translation="MNLSHVESDLVNEIKLAPLEAKVFLLVTTTGKMQADKIATSLGV
                     SGDEALATAKKLVELGGFIDISPTEFEAMHPRFTAVNMYRKMCLRENITFKKNLVVDN
                     IGVALEKYYDHARTK"
     gene            12393..12791
                     /locus_tag="VGA92_00295"
     CDS             12393..12791
                     /locus_tag="VGA92_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844884.1"
                     /translation="MSVDTETCKHQIVYFGVININFNERTIGSVDAWRCGVCKKRFCE
                     EKQLGIESIVDYVGMPKIEPDEKWAVLVRKLLKGKDRWKLVKLKQNGILKTETPDDKV
                     IDLPIKEYKVYDPNHTSFLIDDYINKVIEI"
     gene            12801..13085
                     /locus_tag="VGA92_00300"
     CDS             12801..13085
                     /locus_tag="VGA92_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844885.1"
                     /translation="MDIEVHINNVKGAQMAAKITGTFTIHNNTFKFSAIAFGRIGGHN
                     IGAKISKTTEKALEKLGYNVDEVIDALQKSLIHGNIILPKDLKKDSFADG"
     gene            complement(13082..13876)
                     /locus_tag="VGA92_00305"
     CDS             complement(13082..13876)
                     /locus_tag="VGA92_00305"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX9844886.1"
                     /translation="MLVEQAEIGIFGGTGIYDSGLLKETREVTVETPFGKTSDLITVG
                     IFKGKKIAFMPRHGKKHTIPPHLINYRANIWAFKELGVKRIIAPSAVGSLKEELAPGQ
                     FVLPSQFLDFTKSRKLSFSEEGKVIHISVADPFCPELQAAILEAAKTQSIPVHKDCTY
                     VCIEGPRFSTKAESRFFRSTGADIIGMTLVPECQLAREAQICYASISTVTDYDVWAEK
                     PVTAKEVMETLSKNVENTKKLLMSIPDEIPQVRNCSCAKALEQAEF"
     gene            complement(13870..14382)
                     /locus_tag="VGA92_00310"
     CDS             complement(13870..14382)
                     /locus_tag="VGA92_00310"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX9844887.1"
                     /translation="MNLQNIIVEYPDFPKKGILFRDISPILKSPAALSHVVDEFARHY
                     HTNDIDLFAGIESRGFPFACALALRYNKGMVMIRKQGKLPGSTVKRSYSIEYGKATME
                     IQKNAISEGQRILICDDLLATGGTAKAAAELIERLGGKVVGFAFIVELTELQGIKKIA
                     KYHCQSLVKY"
     gene            complement(14426..16588)
                     /locus_tag="VGA92_00315"
     CDS             complement(14426..16588)
                     /locus_tag="VGA92_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-associated protein"
                     /protein_id="HEX9844888.1"
                     /translation="MFIKVGRLIVTHPRLALFGIIILSVFTVASLSSPVFADTVAKST
                     SFEKTTLIEYVNNEGVPIKTVRMWLGQDAGTFKSFKTEKGWTGALSVQGLLIFTSEEP
                     LPPGDSVKFGIKTEVQNPGINWRTLDLSGNELTTGRTVAGQQPPPQDSTQTPPQDTQK
                     PATSFANAEFKIIPASPKNGDSIRIIGNSFPPNTVVDFYIDGEKLEDFTTDSSGNLIG
                     RTKIPVNKEADRVEFVLSDQQGNKKTLSIRLDHRDTQITAKPAARLAITQITEMVGPG
                     ETARVNGTAKPGSTITISYKDATGIKIQEAVIEVDAQGNWSHERVIPPDAPLGTRVVE
                     FSDGADTITKILSVSITKSIHVTPSAIRYEPSQTMRFNGTTTSDQSVEIVIKDPIGKE
                     IFYDLLKMNGTGIIDFEFPTEQISLKGTYVIIMSQGDDTEILYVGLGVAPTKQVVAKF
                     DKLNYSTSENAKVVLTGPAKANISILILDPSDKVVPGSADTITLGADGSAEYSISLSG
                     YKSGVFTIVLNYLHSETNAVFAVGLQQGSGKITIQVTKQTYQLGDSILILGSTNKNVL
                     LVLEMSDPQGNVVKRKEAFSDKEGKFSDGTFRIPSDAEQGIWTIKASSGSNLADVKLN
                     VVGTVTQAFVVSVDKSTAYSVGDTMTITGSGGGKTQTVIIKIFDSSDVEITELIVFST
                     ENGSFQTFWSVPPDTVPGTYKIETKIANEVAETTFSVQ"
     gene            complement(16647..18734)
                     /locus_tag="VGA92_00320"
     CDS             complement(16647..18734)
                     /locus_tag="VGA92_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9844889.1"
                     /translation="MRLRKFRVRAYRCIQDSGEIKVGDLAAFVGRNESGKTTILQALT
                     LLNKEELVSELDLCDEMTEELKSEVTLVEGEFALSSNETSLIKERFPHLPEIRALKIF
                     RTNKIVDVQYDFGSVKLSDELNKTLNSWENYTEKILDFISTIPNHIRIQFDTKFFEGP
                     PPDKETYLREMTEFSNILNVLAADEPQILTEWSGYYQDAEKTYNELLVGTTEKTALEN
                     FILEHLHPRFVYFSDYKKIYGNINLDEYIKEIKRPEDLTYSEEFDKVETVRNLFYLAE
                     LDTEKLEEYKRSPPKLIKLLNAASRRLTDRLNPAWKGDPIHVDLRYNPGNVMSVVISD
                     VHKDGTVTNTGLLSRRAEGFKWTFSFIVNFAAETQRAELKEAILLLDEPARNLHPAQQ
                     MGISDLLKNLAGSNQVLYATHSPFMIFDYTPGNLLVVELDKRRHLSRIYYDYWNADDA
                     TLIPILYGLSRGLVESIVDREIGTNSRPVIIVETMADAMYLNAFDKFLEDPNISMNPL
                     NVVAAFSKDSALPLAIFYRNHGHNVFVILDNTQESKKIAEHLKTNEFTDMQIIYLEQS
                     GKAIESIEDLIVPDDYLHAVNQTYEIKLRKEGFRNLTKEEVLAKSKKGIVENLRSIWA
                     EHRDDGWNEFDIEEISRYICEKIALKEADFLSDKTKDQFRGLYRLIAERIRQHQNLMA
                     QVGLNKYQRSKSH"
     gene            18836..19288
                     /locus_tag="VGA92_00325"
     CDS             18836..19288
                     /locus_tag="VGA92_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844890.1"
                     /translation="MAASGVKIAVFVIPIVFSFIYGGAVLSLSLQEPNRGIDFQGTTQ
                     LTSIEITSLQDEYSSSDAITAQVLVRDPGFSCGDLYMTIYDVSSAQRKAVAQGAFFDQ
                     CYDSSGTLPVGDQFSQKVDAVGQYLLEVQMFDQSGDKFLTTSQKFSVR"
     gene            19353..20000
                     /locus_tag="VGA92_00330"
     CDS             19353..20000
                     /locus_tag="VGA92_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9844891.1"
                     /translation="MGRPKKSVIDSTEIKKQDKKKEKKTSKVEKKTQKEDKKILPKED
                     KKDKKKDKKIAKEDKKKEKKAKEESEKTLEEELEEQLTDEEIENFQIEKVDMDKLTNR
                     VCLFLANYEDGIVQSELWKKFKLTSRDGSRLALKLERMGIITREKILENKRWTYVLKI
                     KKTPVSTASIENAPCLVCPVEQKCSLDGEVSPRTCQWIEDWTIIELSKPKKKKEQ"
     gene            19997..20590
                     /locus_tag="VGA92_00335"
     CDS             19997..20590
                     /locus_tag="VGA92_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX9844892.1"
                     /translation="MRLVDAKRDHYRKLARERGYRSRATYKLLELNKSYRIIGPGFYV
                     VDLGCAPGGWTQVAVRLAGNRGKVVGVDTAFVEEIEGAYILKGSVEDESVADEILEYL
                     ETKANAVICDISPNITGHWSMDHAKQISLNYSCSKIMDKILASKGNALFKVFDGEYSI
                     EFRDYLQKKFSRVHLRKPNASRKPSSEMYYICLGYRG"
     gene            complement(20587..21723)
                     /locus_tag="VGA92_00340"
     CDS             complement(20587..21723)
                     /locus_tag="VGA92_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N1)-methyltransferase"
                     /protein_id="HEX9844893.1"
                     /translation="MQLNDQIVTIIEGKTQILIPQDSLKNKVPPRDPAFFNPRAKLSR
                     DLSIIAYSAFLKNFEGPKTFLDGLSGLGARGLRVANELGIQVFVNDLNPSALELSKKS
                     AALNNLENYQVSENEICRFLSSFSEKGRRGAIVDVDPFGSPVKHIDCAIRATIHKGLL
                     SVTATDLQVLHGLFKDACKKKYHGTPIKTTYSNEISIRLILGCINMIAARLDAEITPI
                     FVENNQHYYRVYVRVLNKPDTRNTIGFIAHCRACGNRCATREPPLCCEFCGSKVEIAG
                     PLWIGQLFEKEFVESMQSETPNFSVDKKCEKIIQKCVLESNMPACYYTLDEIAEMNKS
                     SPISLEESIKRLQENGFVSSPTSFNPTGFRTDCKISKIRELLAH"
     gene            21784..22413
                     /gene="rnhB"
                     /locus_tag="VGA92_00345"
     CDS             21784..22413
                     /gene="rnhB"
                     /locus_tag="VGA92_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX9844894.1"
                     /translation="MIICGVDEAGRGPMIGPLVVAGIAIEKSKIKKLAEIGVRDSKKL
                     TPLAREKMYKQITRIVDDYAVSKITPKQIDVHVLKHQLNHLEAIQMAKIIRKLQPSVS
                     YVDSCDVNATRFGQVLAKLAKTGKIKSYHRADSRFLVVSAASIIAKVTRDRAIAKLNE
                     SFEIGSGYPSDAKSVQFVREWFCKHGQMPDFIRKSWAPVKLVVKNYALA"
     gene            complement(22398..23078)
                     /locus_tag="VGA92_00350"
     CDS             complement(22398..23078)
                     /locus_tag="VGA92_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HEX9844895.1"
                     /translation="MEKEDNQIFWLTVDGQKRLATINYVEGNQVYGEKLYKKGGVEYR
                     MWDPFRSKLAAALMIGLDVFPIKQGSKILYLGASTGTTVSHISDIVGHNGMIFAVEHT
                     SRVARDFLDRVANFRKNIIPILQDARHPKQYFSIYGKVDVVYSDIAQPDQTQIAITNC
                     EMFLKDGGYLFMTIKTRSIDVTQSPKKIIENEIKKLHQSFSVIQNMDLQPYDKDHAMV
                     VAIYHAKA"
     gene            complement(23062..24297)
                     /locus_tag="VGA92_00355"
     CDS             complement(23062..24297)
                     /locus_tag="VGA92_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX9844896.1"
                     /translation="MYFVVLTELGIVVLENGKMVNSFAFAQPASDFVKVKEGQLRLGS
                     LIDFLRRTNSVFLLSDESLLKTLKKESLDVQLMDEKQMEEIQTNKPQILIDCGFAKDQ
                     ADAMSKLRDFALQLSAAKVTEVSQSPDLHIIQSIKALDEIDVMVNGLSSRLREWYGLH
                     FPELDNIIDSINGYAQIVMTGKRDMLSKQVYEDAGFPESKVEMLSVIQDKSKGGDISD
                     QNLVIVQSIAKQILEMAQIRREIESHIENEMKTIAPNLAIILGTAVGARILAKAGSLK
                     KLACMPASTIQILGAEKALFRSLKTGTQPPKHGILFQHPVVHASPKWQRGKMARAIAS
                     KAAIAARVDVYGGGINHTLLEKLNVRVTEIGQKYKEPSHRPIPPQRIEKQRDNFRHRG
                     PRRDFRADLKKRKKFGKRR"
     gene            24386..25294
                     /locus_tag="VGA92_00360"
     CDS             24386..25294
                     /locus_tag="VGA92_00360"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX9844897.1"
                     /translation="MNPDLSVLIGSISLKRPAMLASGILGISLEVFERIHKSGAGAVV
                     TKSLSKEPWDGYPNPTIVGIKGGYLNAVGLSNPGAPYFAKMISKNTDVPIIVSLVGSL
                     EDEFEFMVKQFEGIKVVAYELNLSCPHVDKVGLEVGDDPNLVTKIVKRIKSTTKVPVI
                     AKVGLGTTNYLDTVAAAIDGGADAITAINTIRAMGIDVETQRPLLSHKIGGLSGTAIK
                     PVAVRCVYEIASEYDIPVIGCGGVSGWDDAVEFILAGASAVQVGSAIGDRWIDVFSEI
                     NDGIANYMKKKGFSKIQEMVGLAKRF"
     gene            25278..26090
                     /locus_tag="VGA92_00365"
     CDS             25278..26090
                     /locus_tag="VGA92_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX9844898.1"
                     /translation="MQNASNTPSIRIIEKVIDETPTVRTMIVSDEKLADVLPGQFAMV
                     WIPGVNELPMSVMITEQKGKAAFTVRKRGESSTAMYDLKEGQQIGVRGPYGNSFDIKD
                     GRILLVGGGTGLVPLMRLAKFAKPNNDVTILIGSKTKEEVFFEELAKRILAKNKHKII
                     VATEDGSYGEQGFVTDVLERLVQNEKFDAIYTCGPEIMMYKIVQLANTKKIFVQASLE
                     RMMKCGIGICGSCCMDQVLVCHDGTVFDGEFLKNSKEFGHTHRSKAGIFENY"
     gene            26173..27903
                     /locus_tag="VGA92_00370"
     CDS             26173..27903
                     /locus_tag="VGA92_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9844899.1"
                     /translation="MAHPKIVLTADRTLMSNYRGISLATFFGCAPALDPHRDHNSFWY
                     KILKNQVTPKVLFDFICNTVPHTNGVANYAPYGLRKVEAGLLRDGFSRQDVVVAHPDH
                     VDKFIGPETEVVGTYEMDPLGMGPVTMTFTYGRKQISYDEYYNIYLHKKINEAKKKNG
                     SHAKVISGASGTWQYNYDPEKIEELGLYAILEGELGGIAPEIDGHAGRFFNYLINGEF
                     DNMNPFRKRSDFKVDIKEYQRGDRTIHGRFVNFWDRPEIDEIPDIVEPSMHGMVEVMR
                     GCGRGCKFCDVTLRALRYYPPEKVKKEIEVNIKKGGLKNAWIHSDDIFVYGMDPRTSK
                     QMEPNREALEELFTAVMSTGIVHTNPTHGTLAGAIADEKLIPNISKIIKAGPANLIGI
                     QCGFETGSLRLIGKYADRKLAPFMPEEWHWVVKEGVKTLNENYWVPAFTLIMGLDNDE
                     TPEDSWETIQLISELEREQPEAMFTTTPLTFVPIGLLEKSEFFNIGNEMSPAQLGVMY
                     KTWQHNFKYGIQKFMTKTGASQTAMQRKFFNGLARSLGGVPLSAMEKFARRKSKEHEQ
                     VIEKIKVKYW"
     gene            27949..28131
                     /locus_tag="VGA92_00375"
     CDS             27949..28131
                     /locus_tag="VGA92_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S30e"
                     /protein_id="HEX9844900.1"
                     /translation="MATHGSITKAGKVKGQTPKVEGRKRIGTIAILRNKSNFRKRFSL
                     HRTPGQNKPGQRKRRR"
     gene            28369..29526
                     /locus_tag="VGA92_00380"
     CDS             28369..29526
                     /locus_tag="VGA92_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844901.1"
                     /translation="MGLGIAIAGGIICATVLAVFSIVFSTSGQIYEINSSRTSAADLQ
                     SVVFQTDMAVSDLTAQSGSQFVNFTLTNTGNEKLWNYEKFDVIVNYTADVLGFPTQRI
                     ESITYTTNSDDISIEAVSSNSQTCISCTLSHTVAGTNKLLVVGISISDDTAVSSMTYS
                     GQSLTLIRFDEVLSTDRRSELWYLVDPPSGTADVVVTLDQVERAIIGAISFTNVDQSN
                     PINVDNGAIDSVGATHPSVSLTTTVDNTLIVDVVSTVQGSMTPSTSQTERWDLVRAQL
                     AGSGSTNQTTTANTYTMSWTNEGGLDQWAMSAAAIRPADPACGPDGTIATNNWLLNSI
                     SNDYADPNLVNTNESALICTSLAYPVYANGDVKVTVSTDNGYTKSSSTTAT"
     gene            29712..29891
                     /locus_tag="VGA92_00385"
     CDS             29712..29891
                     /locus_tag="VGA92_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844902.1"
                     /translation="MKKCVVCGKEFDGIERDVCSWQCDENRLARLRKKLDDAVKNDKG
                     HTKKLSEDSNETTNR"
     gene            30411..30647
                     /locus_tag="VGA92_00390"
     CDS             30411..30647
                     /locus_tag="VGA92_00390"
                     /inference="COORDINATES: protein motif:HMM:NF037845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844903.1"
                     /translation="MSDNLLLVKFRFDGETKSQVGKFTYEQYDNLRSLPITVECKIIS
                     NEKPTLSKEEIDIINQKVIDAWKADRSHTSKLAK"
     regulatory      30705..30781
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            30796..31188
                     /locus_tag="VGA92_00395"
     CDS             30796..31188
                     /locus_tag="VGA92_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX9844904.1"
                     /translation="MKIIELAFLAIGGVAGTFLRYKILTAPYYIGALQANVLIVNVIG
                     SFILGIFAVLSQQWNLDGKYALLVAFGFCGSLTTMSALAYDAHDLLNNAKLGLMAVNI
                     LANVGLSVAAIFGGKALFNLILGNDPLQ"
     gene            31731..32732
                     /gene="radA"
                     /locus_tag="VGA92_00400"
     CDS             31731..32732
                     /gene="radA"
                     /locus_tag="VGA92_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HEX9844905.1"
                     /translation="MVDDIRLDSLDGVGPVTTKKLSDAGIHNIYDLVIRGPVDVAEVT
                     GMDRDTADKIVTKARHLLMEQGLITKDFVTATEIYKRRLDIGKITTGTQCLDDLLDGG
                     LETQALTEVYGEFGSGKTQFCLTMCVMAQKTKEEGGLDGGVLYIDTENTFRPERIVSI
                     AKAKGMEPEKVLDNIIVARAYNSAHQTLILEEAGPIIEQHKIKLIIADSAVGLFRSEY
                     LGRGTLSERQQRLNRFVHLLVRTAETYNCAAIATNQVMASPDVFFGDPTRPVGGNVVA
                     HTSTYRIYFKKSGKKRIARMVDSPHHPEEEVIFTVTEGGVADPEDDTKKKKKSEESE"
     gene            complement(32762..33139)
                     /locus_tag="VGA92_00405"
     CDS             complement(32762..33139)
                     /locus_tag="VGA92_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX9844906.1"
                     /translation="MAKRKQPGYIEKFLKRADKAVDEALTQGVKRADEILADAVEFGK
                     IAAKEADRKSRDLRKQAKIEGAKLKSKGEQEIIKGISTAKRLASTDKADLETLAKLGE
                     LRKAGIITESEFQSKKKKILGRI"
     gene            33225..33932
                     /locus_tag="VGA92_00410"
     CDS             33225..33932
                     /locus_tag="VGA92_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844907.1"
                     /translation="MVNKDYKSQVIVVRKKIDEGKTRQMVEEKKTSLFRSLLKKPAKD
                     EVHIHSIGLNYEAILMISGRYVADFYRKATHPIKVDYNVTQVVLGDGVFPIQIKSRWK
                     TALGGKKGKNKVDLELEEHVSIDDECTLYFDHHGRQTKLPLKLDPKNMENYPTKILQN
                     TKFIVKKPELTHKDAISKLEEELKKPIGTNARNLNEKITITEISEIYIPIYEARLVGP
                     KKKVEILRIDAAKNKIL"
     gene            33979..34281
                     /locus_tag="VGA92_00415"
     CDS             33979..34281
                     /locus_tag="VGA92_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX9844908.1"
                     /translation="MTTKKPSGHSHGFRHKSRSALTKDAPRGVSFLLREYHVGEQALV
                     IIDPRQHKSLPHRRYHGKVGTITSVGRRAVTMDVKLGNKTKTLITRLDHIKPFGVK"
     gene            34283..34609
                     /locus_tag="VGA92_00420"
     CDS             34283..34609
                     /locus_tag="VGA92_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4"
                     /protein_id="HEX9844909.1"
                     /translation="MEQVKKSQPISLAEVKDILGKEDVEKMDQIQRWTYDYVTKFTKV
                     DAKAAKKIKQELKKDCGLTDEEATEIVNIMPSSLAELRAFTFGWKKLILAETLEKILH
                     ILKGNS"
     gene            34628..35194
                     /locus_tag="VGA92_00425"
     CDS             34628..35194
                     /locus_tag="VGA92_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HEX9844910.1"
                     /translation="MERSTSPPRKYEEYAYVLDYVTRGKSTTVRGREGIIITAIGEDR
                     LTLLEVLGVPNAAFEIGERIYIGKEGRTKILSVLGKLEYERISAAAQSELKTVVQTIV
                     TNNEKRFIDYINHSQPLTPRIHALELIPGIGKTYMKTILEERERQPFTDFGDLQNRVG
                     LKEPAKQITQRIMEEITGEVKVTLFVKK"
     gene            35191..35892
                     /locus_tag="VGA92_00430"
     CDS             35191..35892
                     /locus_tag="VGA92_00430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEX9844911.1"
                     /translation="MNKRQRLGQHFLKSVNIAKLIVESAQLTKKDVVLEVGTGRGILT
                     PLLARKAKSVVSVESDEQLYSDAILQFSYIKNLELMYGDGFETDSDFTVFVSNLPYSE
                     SRHAIEWLAQKKFSRAVIMVQKEFADKLASMQKRQMRAISVIANYAFEIEKIMAVNKN
                     NFEPPPKVDSVILRLRQKKQVTPELIESVNGLFSYRRKTISNIAKKFGKKIQSDKRLE
                     DLSGDEIIKLAKQIR"
     gene            35873..36400
                     /locus_tag="VGA92_00435"
     CDS             35873..36400
                     /locus_tag="VGA92_00435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HEX9844912.1"
                     /translation="MQNKSAETYTPAEDTFFLAEHIAKEKGESALEIGTSSGYLSGVL
                     QKNFDFVVSTDIDFGVLSGQKGKTQNKICCDGANALGNKFDLVVCNLPYLPSEKIIDK
                     TTDGGPEGLLVPLGIIKSASICIKSGGKALFLTSSLANYQKLIEKAQSLGFSVTITAK
                     KKLFFEELIIIEMRN"
     gene            complement(36557..36742)
                     /locus_tag="VGA92_00440"
     CDS             complement(36557..36742)
                     /locus_tag="VGA92_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844913.1"
                     /translation="MQSCNDICQRYRARQVTTHSKYGEGQKWCSVCSIFLVSDTIRCP
                     CCNIKLRTKARVKKRMI"
     gene            complement(36771..37247)
                     /locus_tag="VGA92_00445"
     CDS             complement(36771..37247)
                     /locus_tag="VGA92_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX9844914.1"
                     /translation="MKQIDIGISDKDRQEVNRILGKILADEYVLYTKIRNYHWNVVGP
                     HFSEYHKLFADQYAALDGDIDEIAERIRSLGEKTPATLAEFGKTSSIEEHPGSYPDAQ
                     AMVSNLVADHESIIRTLRADVESCVKHSDVGTADFLTGLLEKHEKTAWMLRSVLEK"
     gene            37348..37779
                     /locus_tag="VGA92_00450"
     CDS             37348..37779
                     /locus_tag="VGA92_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9844915.1"
                     /translation="MKQHLEELVTSLRKEGFRITPQRIAIVDYVMNTEEHPSAEHIHK
                     VIQRQYPMVSLSTVYKTLELLREKRLLNEIEVNGESRFDPNTGEHVNLVCLNCGIVED
                     SDEGGLREIQNKIARKSKYLIIRGSLDLYGYCSKCKSKFER"
     gene            37874..38185
                     /locus_tag="VGA92_00455"
     CDS             37874..38185
                     /locus_tag="VGA92_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844916.1"
                     /translation="MSKVLQNKKSTPSQAKTKTSTANPTLDTIKMVEQALSKMSEYTS
                     KNRLWRALPRQVQYPTFKAILDYLEESNKIMYDKDGTIIWIFADNPKLKKLMRTSKSL
                     L"
     gene            complement(38187..38510)
                     /locus_tag="VGA92_00460"
     CDS             complement(38187..38510)
                     /locus_tag="VGA92_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844917.1"
                     /translation="MSKRAKDVIGTKEFRKLFNQISENEPQKRELHNAFKTLQEDCLC
                     GNKITHDLWPQSYIKKYKIKNLWRLPLRSGWRLVYTIMGEKDGFVVCIIEAFSHKEYE
                     KRFGY"
     gene            complement(38606..39634)
                     /locus_tag="VGA92_00465"
     CDS             complement(38606..39634)
                     /locus_tag="VGA92_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX9844918.1"
                     /translation="MAKQAAIIKGDGTGPELVDAMLHVLKSCNTQAELILCDAGSEQW
                     EKHGGATYIPDSTMKLLEESNACYKGPTTTIPKPNAPRSVAVTIRQKFELYSNIRPIK
                     TYDRLSPDKKLDFVCFREATEGLYAGIEFRISEDAAIAIRKTTRKGCQRIVKAAFLWA
                     KQHNLKKIFAITKRNILKETDGIFWSEVEKAIKENSGIDVEEYYIDNMTQQLVKNPER
                     FNNSVLLSTNLFMDIISECASGNIGSIGNVYSANIGDSYAMFEPAHGSAPKYKGMNKV
                     NPTAAILSGAWMVEYLGEPQIRNAIFDATEQVINEGKYVTYDIGGTSTTTQMTDEICR
                     IVKSKLRK"
     gene            39793..40536
                     /locus_tag="VGA92_00470"
     CDS             39793..40536
                     /locus_tag="VGA92_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9844919.1"
                     /translation="MIIVQLSDIHVGSQFREDVFEKVIREVNALKPDAIIVTGDLTNE
                     GLPKEYEKCKKLLSRLETKKIITISGNHDYRNTGYLLFKKHFPFETVNELGSDIVVVT
                     LGTARPDRDEGEVGYRQNLWLERTMQKYKDKIKILAMHHHLIGIPDTGSARVEVIDAG
                     DVLRTILATKVNMVLCGHKHRPWIWNFKELSIVNAGTAASERVRGFFENTYNIITIKN
                     KKIHVDLKIVGGKKIPLESIVEHYSRSEE"
     gene            40570..40653
                     /locus_tag="VGA92_00475"
     tRNA            40570..40653
                     /locus_tag="VGA92_00475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40604..40606,aa:Ser,seq:gct)
     gene            40678..40860
                     /locus_tag="VGA92_00480"
     CDS             40678..40860
                     /locus_tag="VGA92_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844920.1"
                     /translation="MPDPYWNMGRRKTQKIIRTAPKNVTTGLCPKCQTVGRIFLLGQV
                     GAEHAKCAACNQEFDL"
     gene            40925..41098
                     /locus_tag="VGA92_00485"
     CDS             40925..41098
                     /locus_tag="VGA92_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019113026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844921.1"
                     /translation="MSTTEGETIYERVAKLEDEVAVLRNEVDILKKALRNKLARHEIS
                     QIRKGKDISSIID"
     gene            complement(41088..41912)
                     /locus_tag="VGA92_00490"
     CDS             complement(41088..41912)
                     /locus_tag="VGA92_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX9844922.1"
                     /translation="MNDKFDPFVAEWVSFSSDSKYNLVEKCLKLGQILEYPDLNIEDQ
                     VEKINQIAKSLQVMLSDVKNPTYLISMLNEHLFTRLGFSGDTEDYYNPKNNFLNEVLD
                     KKSGIPIILSIIYVEVAKRIGLDLKICGFPSHVVVKYGEEMVLDPFGGGRPLSVEDLE
                     EILYRNFGEEIEFSPEFLDELPEDKVLIRIIRNLKNSYAQSYAYEKALRCANMILAVE
                     PDSPDEIRDKGIIQERLLNYEDALQYLNKYLEIAPEAPDVDFVLELIRNTREKINR"
     gene            complement(41921..43108)
                     /locus_tag="VGA92_00495"
     CDS             complement(41921..43108)
                     /locus_tag="VGA92_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844923.1"
                     /translation="MSELEEFADALLDQISVETNEEKDIASLASKIAEDPDFKVRFDS
                     PRQITELVLPGLKQSVAEFTGISVPDLKIQYPDLEELKNIKGRKVFSTQDSQEFVARL
                     FAALAKQDSGAIVELIKQDTTKFLVYSTYAKAYISKISTTYGDYLDFTIFINNFVLSN
                     YPQIILHKQGKPYELQYETVKSGYIGALKMTVLEELVHSIQQNLYEQNKKAVIEVNKI
                     NEELAKIILNLDDSTATKLADYLQLPAVPDDFPLAKRANLFFTLNPDNFIVNVLGPDV
                     MTFTKVEVDPGISQLVPQLLDIYQRWLEPIQRHHAAFSTMEGMAEFCVQNILKDDDDF
                     AQYLTTFMGTDVSSYQVRKHMGKDLVEAAFSKLGKSAFSVLIQNPPTTRELKQTELYL
                     KRI"
     gene            43188..44069
                     /locus_tag="VGA92_00500"
     CDS             43188..44069
                     /locus_tag="VGA92_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9844924.1"
                     /translation="MHKFSLGYLRCVRCHSTLDVEILREGTQIEEGFLLCPKCKMYFP
                     IINAIPILWNDFANYLSNRPRLGGEFLTSANTSKMRSFVKQTLSKIRKNPSDQSIVEK
                     RWSEIYKQNKNSTFYCKIKEHLRPIANSGVVLEHGCSIGIMTQHLAKTNRLAFGIDKS
                     YYAISEAKKSKLKNLDFFVADSLEHPFGKTKFDLVLGLNLFELIEPKPLLKLLAGQVR
                     KKEWLVLSDPYDFDRGAKSVKEPLYEDSIRKEITRQGFAISKKTTKPSHLLWDLRLYS
                     RANLQYKVDLIIGKKTR"
     gene            44141..45310
                     /locus_tag="VGA92_00505"
     CDS             44141..45310
                     /locus_tag="VGA92_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844925.1"
                     /translation="MGFSHVVTAGIVIIPLMLIAMNIPNISNTILEINEAALDVSELE
                     DAILNTHFQILNLQTTGLNNRIFFDLTNTGTTKLWDYENFDVILTYEGIVNSVATNVT
                     EVLSYSELAPSDDPILFDAVTSHTDSCLVILNPCTFSHTVTNSGSDRILILAISKQNN
                     AVLSTVTYDGEDLTELRADDVDGNTNAHTSLWYLVDPPTGSNTVFIGVGLFDTDVVVG
                     AISFTGVNQTDPFDAVNGATGNSATAATTLTTTTNEAWLVDVVGTNGGTVLVGAGQLE
                     RWNDVQASVRGAGSTEFTSSAGTFSMSWTKSASSNWAITAAALKPANAACCVSVGNWA
                     INNISDDYIDPGIINKDETAAIRAKTSYQIITNGDMYVTVAAENGVRASSFVNAQ"
     gene            45411..45632
                     /locus_tag="VGA92_00510"
     CDS             45411..45632
                     /locus_tag="VGA92_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844926.1"
                     /translation="MANFKALKESLDKNGELMIRLDTGEKIELHKHNVSFNDSTQEVI
                     VDAATEAYWIDAGKIAYYWIHREGIEGKE"
     gene            complement(45722..46588)
                     /locus_tag="VGA92_00515"
     CDS             complement(45722..46588)
                     /locus_tag="VGA92_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844927.1"
                     /translation="MEELVVKKDFSDIYTKKYPAPYLEEMNHVEYRIPDQTRPLYKHL
                     TERMGNHLKRSINILDLGSSYGINSALLNYELIMSELNDFFIQQNPRPSIKEVQDFFD
                     ELPGKNPNLKFYLVDTSSPALEFAEKAGLCEGSFCVNMEKEPLSSEFKLTLSNIDLII
                     STGCIGYIGWKSFEKMFENIDDQNSSTIFAFTVLRMFHMDEIERVFKKNNFELIKTKI
                     GPLKQRQFYNEEEMKKTIELLDRRGVDTHGLEDTGCYFADFYMAGHSHMKPIWTPWVQ
                     NLEDVFVPIQGI"
     gene            complement(46715..46915)
                     /locus_tag="VGA92_00520"
     CDS             complement(46715..46915)
                     /locus_tag="VGA92_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844928.1"
                     /translation="MRFSNKDVETRSRLLKKMEEDRKHEKIRIGQRRKAQLRGKTISR
                     ITQKRRKYTVRRKAAKKKSSVR"
     gene            47050..47448
                     /locus_tag="VGA92_00525"
     CDS             47050..47448
                     /locus_tag="VGA92_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9844929.1"
                     /translation="MKSGLSVILVKDIMSRALITVDASTTIYQVAKMMEQGRLGAIIV
                     KNDSHQGIITDRDFAIKIAVNKHPLDTPVHKVASYPLQTINSSESILAAADSMSAKKI
                     RKLVVVEDGKVVGIITSTDLVNQLAKMKND"
     gene            47621..47908
                     /locus_tag="VGA92_00530"
     CDS             47621..47908
                     /locus_tag="VGA92_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844930.1"
                     /translation="MDTSVIIALADPLDVFHEQSMSFVNKLVMYTIECMSGSPLIMEL
                     GKLVEAKGTKRCLDIINSMEEFGIHLKNIDMKQAWKLSEIYLDEGVLTKRH"
     gene            48084..48251
                     /locus_tag="VGA92_00535"
     CDS             48084..48251
                     /locus_tag="VGA92_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844931.1"
                     /translation="MTKHDNLKMLDQIRKELSQDLEKMTSQQREEFFKGTEEIYEGLK
                     KMAKVRQSITR"
     gene            complement(48297..48401)
                     /locus_tag="VGA92_00540"
     tRNA            complement(join(48297..48339,48370..48401))
                     /locus_tag="VGA92_00540"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48334..48336),aa:Met,seq:cat)
     gene            complement(48476..50041)
                     /locus_tag="VGA92_00545"
     CDS             complement(48476..50041)
                     /locus_tag="VGA92_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX9844932.1"
                     /translation="MKWKTLEHNGIIFPPDFESKRISVKIKGQPVKLDTLQEEMIYQW
                     AKKKDTPYVQDTVFQKNFVADFAATFGGKFKGISISEIDFSEAFKFVDKEKDARLLVT
                     KEEKKSLAQKRKELREKMKSVYGKAILDGHEVEVANYMAEPPGIFIGRGDHPLRGKWK
                     PKITHKDVTLNLGKEAKVPPGNWKKIVHEHDSMWLACWDDFISGKIKYVWLADTAGLK
                     QERDQAKYDKAAKLGKEIKKVEEKIISAMKDRNPRVSKIATACYLIYRTSMRVGDEKD
                     TDEADTVGATTLRKEHINLGNDSIHFDFLGKDSVRWEETIKCQGNDVMLYENIKKLIS
                     NKKPQDEIFDGITSRHVNAFLGEVVKGMTAKVFRTYLATTEVKNYLKNNTNVKGKSEN
                     EKLYIAKMANLQAAMMCNHKRTIPKNFDATLEKKKEQLKKLEHTEPKTDKQKEKLKER
                     REKLKLAIELQRQTRDYNLGTSLRNYIDPRVFKAWTAEVKADWEKLYTTSLQRKFLWV
                     KNADAKWNDIVAQ"
     gene            complement(50114..50941)
                     /locus_tag="VGA92_00550"
     CDS             complement(50114..50941)
                     /locus_tag="VGA92_00550"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9844933.1"
                     /translation="MQKVALVTGSSSGIGFETALALAREGYYTYASMRNTKKSKPLLD
                     EAKKENLPVSVIALDVDKPESVKSAISQIMAEKQRIDILVNNAGYGLFGCLEDLSIDE
                     IKEQFETNFFSIIRIIQEVAPIMRGQKAGTIVNVSSVAGRIAFPGTPAYISSKFALEG
                     LSESMRYELSPFGINTIIIEPGAIKTNFFGALRMPKNAKPDSPYKDLTDKVVNGIKMM
                     AEMGTPPKEVADVIVKALREKDPLPRYPVGNDAVMFLEAKKMKTDIEFENYLKKELF"
     gene            complement(50998..53508)
                     /locus_tag="VGA92_00555"
     CDS             complement(50998..53508)
                     /locus_tag="VGA92_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9844934.1"
                     /translation="MRYSCPKCKSELQIQKTFNKKYMIACQKCDLQDLVDYTKNIDEV
                     YLEFLARYDQGETPTKKQLGLELKTAGIIRDKKEIEKMIADSSPDEITRDVLFSKKDY
                     VSFYKAIKEPEPALGAPVDECGLDDGIIQHLQQKKIEQFYKFQEEAINEIIFGQNVVI
                     TAPTASGKTEAFAIPIIQKIANEVQSGAILAIFVYPTKALSRDQLPKLREVAQRVGVR
                     VEVFDGDTKQKERAQILDEPPQIIVTNFDVLHYHMMYRTRFASAFSTAKFLVVDEAHV
                     YSGIFGSNVHYIIKRLKRICKKIQFVASSATLENAKEFCEQLFGVKMQLIVGSGKKGE
                     TDFVMIFPSLRTQRALTVELLEKLTAKKHKTMVFSNSHLNSELVAMQAKKKKIDIRVH
                     RAGLMANYRSFVEKSFKEDRLMAISCTPTLELGIDVGNVDGVISSTIPINRLLQRIGR
                     AARKGQKGFAFLALGNDPISQYYKNHPDDYFEDVEKTYIDPKNPFVEEFHVLAMAADK
                     PIARHELSEHVGVIRKHVECGNLVLKEDRYVPNFDSVKSIFSDYSIRGIGKSIDIFYN
                     DKKIGDRVLPMALEELHESAVYFLAGTRYKVKEFGYPQKQYAKLAPLPSNYPYYTKAL
                     TEEWPTIKTVYEKRQAVGIEVCFCKLHIQKRVYGYVNIELGQEVNQGQKILLEQPLEY
                     DFITKGIVIHAPVPQNQIQLAENPEYVEASGYHATEHIIIEGSNMVTGGVSQDLGGIS
                     LGTSGLIFIYDGAIGGNGASKALYDRLEKAFERSLSIVRECPCKNEAGCPRCTFSYRC
                     GNNNEFLHKGSALEILQRINDGEVTEIIDPTEGDRPLV"
     gene            53713..53919
                     /locus_tag="VGA92_00560"
     CDS             53713..53919
                     /locus_tag="VGA92_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844935.1"
                     /translation="MGNSSGSYVRGVAAIHKKYQTALKHARSRQSVLNAYWRHKKESE
                     RLLAKHLRDEMAEVKRIKGKMEYR"
     gene            complement(53969..54706)
                     /locus_tag="VGA92_00565"
     CDS             complement(53969..54706)
                     /locus_tag="VGA92_00565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9844936.1"
                     /translation="MNCIDGMQHILPKTIDLIVTDPPFAIDFRATKQNYNRTASRVMQ
                     GYNEIKQKDYYDFSVKWMEQAFRILKESGSMYVFSGWNNLKDILNALDDVGFVTVNHI
                     IWKYQFGVVTTRKFVTSHYHCVYVCKNEKKRKFYPFTRFSQKQKTSEGRSLHYKDKED
                     VWDIKREYWTGDKKTPTKLPAEILRKILEYSSRQGDVVFDPFLGSGQVAMISKQMGRN
                     YLGFEIVKKYHDFAKKRLDKNMYRVKA"
     gene            complement(54742..55089)
                     /locus_tag="VGA92_00570"
     CDS             complement(54742..55089)
                     /locus_tag="VGA92_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9844937.1"
                     /translation="MLEYSLNILGGEWIIIIFVALLALLGTNRLPEVTKKLGRVVGEY
                     NRTKKEIQSQISGVVNTNLDVIAPVQNERQKLEFIAKSLGVEFTGKTNDELKKIISEK
                     MSTQSDTTQKDRS"
     gene            55217..>56259
                     /locus_tag="VGA92_00575"
     CDS             55217..>56259
                     /locus_tag="VGA92_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844938.1"
                     /translation="MKGLIAISAILIATMAIAPAYAVIVEFQTDKTSYKKGDVIKFSG
                     KTDIAHSNKMVSVKVYGPDGEFVMLTGGRSDQDAILVLNPIDTSKEKTVAKFSQKGIY
                     NVTAFYDLEPTYAGKSTLFDYSPDGSPVSPSAAEVMGQQAQQPTQTPTQQPTQTPTQT
                     PTQQPTQTPTQTPTQQPTQTQQETQEPKCGPGTVLNKDGVCVIAEPEPEPEAEPEPAP
                     EPETSGPMVTHIPGFPDPDKDPQYYIDRYNNEPAYKEWFDRNFPDYTIYQVLGVLGPK
                     KTHIPGFPDQDKDPQYYINRYNSDAEYKAWFDRNFPDDTIYAIVGVEEPDSEQTDGCG
                     AGTHLEDGVCILDG"
BASE COUNT        16605 a        11670 c        11887 g        16097 t
ORIGIN      
        1 atatctgagc ctacgaaact ttactcacct attgttaaaa tcaagtctct gcccctatca
       61 ctgaggcaaa atggcgatga gcaacttgaa aagtatgaat tgttagagct tgaagacctt
      121 gccagcctcg caaagataag ctacggctac gatgaggctc cgtttccatt gtttgcattc
      181 cctcacaagg acaacaagtg gcttttaggg gtttttatga atttcaatga agaaggacct
      241 tcatattttt gccatgtcgt gcttgactca gagccatctc gaccatttct caagtacgca
      301 atgcacaatg gcaacatgcc agtctttacc gacaatgtaa atgagcatgg ctacacatac
      361 attaagataa taaaattgag ggaagcacat ccactagttg attatgtcta gtttcaaaga
      421 acggattaaa aagctcgcct ccgaaaaaag ctcattagtt ttagcaaatg actatgaaaa
      481 ccacaacaca atactagaat ccaaaacgct tggtaacatc aaaacattaa atcaatatct
      541 ctgtgcaata aaattcaatt ttcatctttt gttgccgctg agccaaaaac aaatcacaaa
      601 gataaacaag gcagctcacg attcaggcct gcaaacaatc gcagacatta agctaaatga
      661 cattggcaac acgaattttg ttgcagccca aaccctttgg gatttgggat ttgatgcagt
      721 gatagtaaac ccaatgatgg ggccatcaag ccttgagaat ctagtcaagg ttgcacacaa
      781 aaacaaaaaa ggaataattt ctctctgcca tatgagctca ccagaagcca aagtcacata
      841 cgagctggag gttcaaacgc aatcaaagaa aacaaacctc tacaagttat ttttagaatg
      901 ggcaacaaaa actaatgcag acggaataat tgttggtgca acatatcctc aaatagtgaa
      961 attctgcaaa caaaaaacca gcggcaagat taacatctac tctcctggta taggcatcca
     1021 agggggagat atacaaaaga caatccggtc gggctctgac tatctcatag ttggaaggac
     1081 aatattgaaa tcaaaaaacc caaaacaaac tgcaaagtcg ttatttgatc tagctggcaa
     1141 aaactaggct ctttgatttt ttcaaaagtt cttttgcgtt ttgatacgtt gttttcttga
     1201 atgcttcatc gaactcaagc catgcgtaat tgatgtgctc attagaaagc ttgactgtag
     1261 tagtattggt ctttgccaaa aagaagacga cctcttttcg cttcactttt ccaccaaatt
     1321 gatagctgta ctgtattttt tgctcaaaac cgtctatgaa ttcaatgtcc gttattccag
     1381 tctcctctct tgcctctcgt atggcagcct cctttggccg ctcatttttt tctattcgtc
     1441 cctttataaa gtcccaatgg ccggacggat aatgcaaaag caaaaacagt ttcttgccag
     1501 actcttcaca aaatagtaaa attccagcag acctctcatc aaactctgac atttattttc
     1561 ctgtcctcct ctcccttttc tgaaaaattc taatcgccct gagtagatca atctttctaa
     1621 attccggcca caaaatgtcc ataaagatga gttcactgta tgctgcctgc cacaacaaaa
     1681 acccgctcat cctttgctcg ccagacgtcc taagtatgag gtctggagac ggctgtggaa
     1741 ggtgtgcagt atagaggttt gcctcaatta cgtccttgtt gatgtcatta acaccaatac
     1801 ttccatcctt gatcctagtt ccaatctttt ttatggcatc cacaagttcg ttttgtcctc
     1861 catacgcaat tgcaatgttc agatagtgcc ggttgtaacc ttttgttgcg tcatcgagcc
     1921 tttgcagaac atcattgatg tagtcaggca aaagctcagt tcttcccatt gcctttacac
     1981 gcattttgtt tttgtgaatt cttgcatccg tgtagagttt ttcaagtctt tgatgaataa
     2041 gcttaaacaa atactccaac tcctcgtctg ttcgatctag gttttcagtt gataaaacat
     2101 acagcgtgat gattttgatg tcgagttcct cacaccagtc aagaaggttt tctactgcat
     2161 cagcccccat aaagtggcct ttttggcttg tttcaagtat cttttttgcc caccttctgt
     2221 tgccatccaa gattacagcg atgtggtttg gtatgttgcc ttttctgatc tctttttcaa
     2281 gtcttcttgt gtatagtctg taaaaccaag agaggtaaag tgttgttttt tttattccgt
     2341 taatacctcg ccacccttct tcttcctata tttgcggaat aagaaggtgg cagatatcag
     2401 tgttattgca agacctgcaa ggaatggcgg aatcaacgta aatgaactct cgtcgtgtat
     2461 gagcaggttc gtaaattggt gtacagtggg atacaacgaa acaagcacaa tcattcttag
     2521 aatatcagtg atttgtctaa ggcttccctt tagccagtga ctgagcaaaa ttccgccaaa
     2581 aaccgaaccc atacccatcc acaaaaatgg cagtgcacct gcaatgaact gacccagtgt
     2641 taccgtgtct gcaagtatgt ttggaatgcc agagtcaaga atgtgtggat caagtatgtt
     2701 tgtattaatt gcagaaaatc cagaaagaac cgctgccaaa ccgatgatta ttcctgcaac
     2761 catcgtaaat gccctgatta gacttgccgg cgttggtttt gcccagctgg acagggtcct
     2821 atctacatca aatgccctga tcaaaaatgc gccgcccaaa atactaacta aaactgcaaa
     2881 gatttcttca gtgtaaccag aaattgcccc aatgcctcca attaagagca agatgccagg
     2941 tactcccaaa aagaattttg aatacttgga atcgtatgca atcattttca gatattttcc
     3001 aaacactgca tacgaatact ctacactcct gcttaccctc attaccacac gctgcactga
     3061 aacaattggg acaacatttt gtatgattgg aattacggat tcgtcgtcct ctccgtctga
     3121 tacaatcact gctccatttg cagaatagct ttgcaaaact gccttgactt ctcttacaat
     3181 tttttcgtct gcctgcacac ctctttcttc aactccagct actacgatta cttcggcttg
     3241 gtagcccttt cgcgccaaat cctcgtatgt ttttactgca aagaaaatcg agtttgcgtc
     3301 ggcatcttca ggatcctcta gagctagttt ttgtgcagct tcgatgcatg catttcttcc
     3361 aacaacgggt gtcgctatgc ctgctttctt gccaacatca tcgtctctgt ctacacaaat
     3421 taccagtaac ctacttgtta cctgattttc taaggccttt tccagattgc tatccttaag
     3481 agacaatgct actgtatgtt actaaatcgc ctattaacaa tttccaacgt ttgaaattat
     3541 tactaatagg aatacaggcg tggttatgct ggtcttgcac tatttgagat tgccaagaac
     3601 tcgtcagcct gcttgaggta ctcatcaagc gcagtcatat cctggctaat gtctgcctgg
     3661 ggattctctt ttaccttgtt tgcaataacc atggtttctc ttaggtacga gttgtaggcg
     3721 cgcaaaaagt ctatgtagga cgaatagctg gtttgccatt cttgcggaac ctcaccttct
     3781 atgattttga ttatgaatga gttgatctga gatgatgaaa tctctgcaac actgatgtag
     3841 ctgtctggag tagcttggcc agcaaacatc ttgtcaaact cctctaaagt cgagtccgaa
     3901 atcgtttttt gttgctcaat taacgcatcc aactcttctt ctgcatttga tacaacaaac
     3961 ttggtctggg tgttttgtgg tacgagccag actgtaaaac ttgcaactgt tatggcggca
     4021 agtattatcc cagtgatggc aaagcctttt ttggactgca acgatgtttt atcacaaaga
     4081 tcgccgttta taatacaatt gaaattcaca caagttttgc gatcgttttc tgctaaaatt
     4141 ttctgttttc tgattacaat caagttaatt ggcaaaaaac acaaattatt atataataaa
     4201 tcaaaatttg tggcaagttt tagatgataa atttagactc atccatcgtg tagttaaata
     4261 atttttacag cctcgtgcta aatacacgca cctcgtagcc aaatcataat ggctacagct
     4321 tactgtgtaa aatgtagagc aaaaagggag ataaaagatc ctaaagtgac aaagctaaag
     4381 aacgggcgtc ctgccgtaaa aggcacttgc ccaaaatgtg gcactaacgt ttttcgaatc
     4441 gggaaactcg attagtaaag ctaatcactc tttctttttg acttaagctc atataataga
     4501 gtattctatg ctcagcattg gcaaaaacag actatgaaaa gctcctaaaa cgaatgcaag
     4561 ataaaatctc aataaaaaaa tctgacagtg gagagcgatt tgagcttcct acacctaatg
     4621 tgatgtggga gggccagcgt acaattttga gaaactttat ggactatcca aaaatattgc
     4681 ggcgagatcc agacaaggtc ttgcagtatc tggcaaaaga gtttgctaca ccagcagaac
     4741 gaagcggtga gcaggcgatg tttgttggtc gtagagaccc gcacgacttt gtgaatttgt
     4801 ttgaaattta cataaaagac taccttgaat gcccaacatg caagagccca gacactagaa
     4861 tcgagcggga aaacaggatc tcatttcttg tgtgcgaggc gtgcggtgct aaatcatcac
     4921 tcaaaggtaa atacgcataa tgcgattttt tattcggaca tcaaattatc aaaatagcag
     4981 aatgctaaac atttgcgatg aggatgttct aggaaaggtt ctcaaaaaag accacctgca
     5041 aattaacatc agcaaaagct actacggaca acgacttata gaaaaagacg aagctgaaaa
     5101 tctgatgagg gatagcacga ttctaaatct agttggaaaa gaaacaattg agatgtcaat
     5161 aaatctaaag attggatcac gacagggagt caaggtaatt gaggacgttc catttctaat
     5221 tgttttcaaa atgtaaagca cacaattata tacaaaattt tctctaactc aatgattggg
     5281 aaagagaaag gttctaaatg aaagtgagct aaaagacatc cagctcccac aacaaggaga
     5341 gttgctgggt agagtaatca agcttttagg aagtgatcaa gtacttgtaa aatgtatgga
     5401 tggactgaca cgacgtggac gaattcgagg aaagctcaaa cgccggatct ggattcggga
     5461 caacgatgtt gtaattcttg caccatggga tttcaaacaa gaagacagag gagacatcat
     5521 atggagattt actctagcgc aagtagactg gttaaaaaat aatggccatc ttccaaaaga
     5581 tttctagcct acatcattct tctttgtagt atgtctgaag acgacattga aattgcagac
     5641 gacgactttg atccaaagtt tgataaaatc tttccaaaat caaaacacag aattcttcaa
     5701 gatggcttta acaaagacaa agtagtcagc caagtccttg acagatcaac aatgcttaca
     5761 atgtatgaga tgataaaccg caaaataatt tcatacgtaa atggtattgt taaggcaggc
     5821 aaagaatcag ttttgttttg ggcagtcgat agtgcgggaa aagacgttgc gctaaaggtt
     5881 tacctagtta ccactaccag ttttaaaaat cgcgccaagt acataatcgg tgatccgcgt
     5941 ttttcgaaaa tcaagcacgg cacaaggaac ctagtcttcc tgtgggcaag aaaagaatac
     6001 agaaacctca agcaatgtga aaaactgaac ctgcccgttg taaggccact ttatgtctca
     6061 aaaaatgttc ttgcaatgga gtttgtgggt aaaaacggca ttccagcccc aactctgcac
     6121 gaaaccgaaa tagatgaaaa cgattatcga caggcaatac agatcattac gaggctttac
     6181 aaagaggcaa accttgtgca cggggatttt tcagaatata atattttcaa aacggaaaat
     6241 ggtcttattc tctttgatct cggctctgca gtcgatacat tgcacccaaa tgctcaaaac
     6301 tttcttgaaa gagacattaa aaatgtgtct tacttctttg caaaaagagg cctaacagtt
     6361 gaaaatccgt ctgatgtact cgcaaaaata gtatcatgag ctttgaaaaa ataataagaa
     6421 taccagtaga ccgtgtaggt gcgcttattg gaaaatctgg caaagtaaaa atacaaattg
     6481 aaaaaacttg ttttgtcaaa ctaaacattg acagtgagac aggagaaatc gaaatcataa
     6541 gtgatggctc aattagccag atccaaccct tcaaggcagt cgaaattgta accgcgattg
     6601 gtcgaggttt ttcgccacaa aatgcaatga agctgataag ggaagacaat ttgttgtacg
     6661 taatggattt gagagagttt gccggcaagt caccacaaca aattgaaagg ataaaaggaa
     6721 gaataattgg agagggaggt agggctagag caaacatgga gaatcttact aataccagca
     6781 tctgtgttta tggcaaaacc gtttctataa ttggagaagc aacacagatc aaagtggtag
     6841 tatcagctat ctcatctatt tctagtggaa gtaaacacgg ttcagtttat ggaaaacttg
     6901 aggcgaacag acgaaagcaa aggctaagca gattacagtt gtgggaaaaa gacgatggct
     6961 cttaaggaaa cctttaacca gatatcgcca agcgagttct tttatagaaa tcgagaccta
     7021 gctggcttta gcaaccccac gcgctcacta tacacagccg tgagggaatt tgtggaaaac
     7081 gcacttgatg cgtgtgatca gcaaggcata cttcctggcg tgcacctaat aatcaagcca
     7141 cttgatgccg aaaaaccaga cccaaaaccc tacattctga cagtcaaaga caacggccct
     7201 ggcatggaat caaaacaaat cccactggca tttggaactg ttctttatgg ttcaaaattt
     7261 ggactaaaac aagcaagagg aatgtttggt cttggggcta ccatggcaat cctttatgga
     7321 caaatcacga caaacaagcc agtaacagtt gcgagctctg ctgacggtaa aacagaacat
     7381 gaatatgtca tgatgctgga catacaaaaa aacaaaccag taattgttaa acatccggca
     7441 ccaagacaaa caaacaagaa gggactgtct gtcagtatag tgctagaggg ggactactct
     7501 aaggctggct ctaaaatcag agattatgtt taccagacat ctcttatcac gccttatgcc
     7561 accataacat ttgatggccc aaagggagaa aaattccact acaaaagaat cgtcgataca
     7621 atgcctcccc caccaaccat aataaaacca cacccacatg gtgttgatgt cgaaacaata
     7681 cgacgtatga tagttgacac acactatcaa attcctgtac ttgataacac catgatagaa
     7741 aaagttcgca aggagcttgg actttcaaaa aagaatctta actttgatgg cataatggca
     7801 aaagcagaaa agaaatgggc atctctttca agacccgtgc gggtaattgt cgcactaatg
     7861 tcgtttttgg agatggactt tgaaaaaatt atgaagattc gaattgacga cgttgacctt
     7921 gcgcacaagc acatgacata ctgggacttt ggtgagtcaa aatcagtaat agtggatatg
     7981 ccaaaatcaa acccgtacta caagcagctt gcaaatacag tccaaggaga gtctgttgcc
     8041 acgtttctga caaagagatt tcaacgcata ggaccatcaa ctgccataaa atttgcagaa
     8101 tttgcaaacc tcaagccaga aaaacgaata ggctcacttt ctaatgagga gctagtccag
     8161 ctcagtgact ctctgcaaaa atatgaagac tttctttccc cagacccaag ctgccttgcg
     8221 ccactaggcg aagagccgct caaaaaagga atgtcaaagt tctttgagcc tgattttcta
     8281 gaggtggtgc agcgaagcgc atcggcatat tctgggtttc catttgtcat agagatgggg
     8341 gttgcatatg ggggaaagat cccatccggc aaaataaccg tatacaggtt tgctaacaga
     8401 atccctcttc tttatgatga gggcagcgac gtcgtacttc aggtggtaaa tgagactgac
     8461 tggtcaagat acaaggtaaa aaatgatgca ccactagtaa ttgtaagtca tatctgctca
     8521 actcgcattc catacaagac tgttggcaaa gaaaatgttg cagacaggcc ggaaattgaa
     8581 aaagagctgc gtcttgcact gctgtttttg gcaaggaagc tatccgcata catgtcaaaa
     8641 agaggccttg cagagataga aaaaaagaga accaacctat atcagaaata cctaccactc
     8701 atcgcacaat ttgcaactga tcttgcaggt aagaataaag agcctgacta caagaaactg
     8761 atcaaggatt tgaacacaaa tgtcgaaatc aaagagtaaa acagaaaaag tagcaaccgc
     8821 aaagcgggac tcgctactgg aattgctcaa aggtgagggc tcaaaaattt actcagaatt
     8881 agacaaagga caatttccgc aattttatgt gccaagcagg tcagtaaaca atattgttta
     8941 tgacaaaaaa ctccgacagt atattcttgg caaggcggca agccttcgaa gttcaagaaa
     9001 catggcgcaa ctacgatcat ttactcagct gatttggctt gcgttttttg caaaaaaact
     9061 cgtcaacgag aaaaaatcat caacactaag agatatctac tattcttcac aggcatttga
     9121 aatagacttt gaggaccagc cagaatcgga caacataatt attgatcttg aggcagtcct
     9181 ggcaaggcca agagaggact ttcatatttt cccagaggag agaagcagcg tttttggtga
     9241 tctgacaata gagtacacgg ttccaggata tgagggaagg aggactaacc tgtctgacca
     9301 tccagacgga tacctaattg gaccaagcct tagcagcgca gaactcgttg acacaagcgc
     9361 agaactagta attgcaatag aaaaaggcgg tcttttcaca agattcgtag aagaaaaggt
     9421 tgacaaaaaa ttcaaggcaa taataattga tactgcaggg caggcaccgc gctcaacaag
     9481 acatctctta aaacggctcc atgagcaaat gaagcttcca gttgtgatac tcaccgatgg
     9541 ggacgtttat ggcgaacaca ttgcgatggt aatcaagtcc ggttccgcaa atgctgcaca
     9601 cctcagagag cttacggttc ctgacgcaaa atgggtcgga gtctgggcat cagacattga
     9661 gaagtacaag ctgccaacta ttccaatgac cgagtctgac atcaaaagga tctacgatct
     9721 gcaaaaggat cctaggtatc aggaagggat atggaaaaaa gaactggaga tatttctcaa
     9781 gataaaaaga aaggcagagc ttgaggcatt ctcaaagtac ggcctgacaa atattacaga
     9841 caagtacctg cctgaaaagt tgcaaatagc taagagcctg tagctttttt gattctgagg
     9901 gtgagcacac cgtttctgta cttgaagtcg gcaatttgca tgtttgcagc accttcgatt
     9961 ggaacctctt ttgagaaatt accagagccg cgtatgtaca acactccttc gattagtctt
    10021 accattatct tatcatcagg gcctggaacc tctgctacaa agattgtctc atcatccccc
    10081 ttgataagat cgtaaaccca gtttttgctt tctgtgtccc tgactgagta ttttggcttg
    10141 tcagtctttg tcatcttttt gataataaaa ccccagtaaa tcatagtcgc agcagcaatt
    10201 ccaattaaaa taaagctcac cgagccgtgg ccagaacgta gagacatgat gtaaattatg
    10261 cctaaaaaca gaattatcat aattggtatt atgaaattta tcgattgttc gttagaatac
    10321 gtttttttgt agctggacac tttatctgag ttccttttta ctaaatataa agcatttagg
    10381 ctacttgacg attaaaattc tcaattagta gagtggcagc gtaattgaaa acactgtggg
    10441 atctgtgctt acactgatct tgcctttatg ctgttctaca atgtttttac agcttgcaag
    10501 accgaggcct gttccctctt cttttgttgt gaacaaagga tcaaatattt tgtctattat
    10561 ggactggaca atcccatttc cggaatcctc gattcttatt gttacagatt cttggtactt
    10621 tttggttctt atgttgatta ttcctttttg atctcctatt gcctgaatgg aattcaatat
    10681 catattggca atgacaattt caattttttg tggatcgcac agaaaagtta tttctgcttt
    10741 tggcacgttt actgtaatgt ttgggggaat tagcaaggtt tgaagcgcgg ccatgattat
    10801 ctcatttaat gaggaaggtt ttttcttaag tggagttgct tttacataat ctaagacgcc
    10861 atcaatttgg tgcgtcattc tgaaaataga gccctcaatt agatcaactc gttttcttgt
    10921 aggatcatcg aggctctcgt ttgtgtgttt gattaattga accgtcgctt tgatgacaga
    10981 aagaggattc ctcaggtcat gtgcaagcct tgaggccaac tcgcctattg cagacaatct
    11041 ttctgcctgt actagttttt gcgttttttc ccttatttct ccttctaatc gattcttttc
    11101 ttttttgaga tttttttcta tgcggttaaa ctcctttaga ttttctccaa gaatctttct
    11161 aaactcatac gagttctttc tttcctcctc gagctggctt tcaagaacta aaatgcggtt
    11221 ctgcacctgt ttttccacaa ttgatgtaat gtccccaatg attttttctg actcaaactc
    11281 tactcccaca ttatcaaact cgtttttttt aacttggatt ggaatcgacc gtcctcccga
    11341 atttaaaatg ccgatttcct tttcaaaatt attctgtaat ttcttcattg catcaattct
    11401 ggctttttgg tcaactaggt gttgacttaa tgagccaatg tactgatcga gatttcgaat
    11461 atcgtctccc aaactcccaa attttgcttc ggtggaattg gagctgggat cttttttcaa
    11521 aaaaatctcc ttgtcagttt tctcaaatgc atcagtaggt tcagactgct tttcatttct
    11581 gtttagatat ttccggctca tctgtgaaga aaattcgatt aaatcgaaat taaattgcca
    11641 gttgttcgat tcgaacagct tgggggcaag ggggtttaat tatacaaata cgcatcaaaa
    11701 ctagtctgcg caatcttgat ttttattata gccctaaaac tatacgatcg tggataaaag
    11761 caaactcaat ccgcgcccaa caaaaggcat ttttttgaag agctcactaa ttggagcgat
    11821 aataacaatt ccttcgcttg cggccttttt cataagctgg ttaatcaccg gcgacaagat
    11881 tacctccctc ataataggaa taatagtcca ctttattgga atgggctttt cattaaagat
    11941 atcaaaaaga ctcttcaaaa taaaaacgcc ctgagctatt caaacttaat tattcaaaca
    12001 catttcacta attgtattga acctcagtca tgtggaaagc gatctagtca acgaaatcaa
    12061 gctagctcca cttgaagcca aagttttttt gcttgttact acgactggca aaatgcaagc
    12121 agacaaaatt gctacaagtc ttggagtcag cggcgatgag gcacttgcca ctgcaaaaaa
    12181 acttgtagaa cttggcgggt ttattgacat ctctccaaca gagtttgagg caatgcatcc
    12241 aagatttact gcggtaaaca tgtaccgcaa aatgtgcctt cgagaaaaca tcacttttaa
    12301 aaaaaattta gttgttgata acataggagt tgccttggaa aagtattacg accatgcaag
    12361 gactaaataa tgctttttta ccgaaaaaga caatgagtgt ggacacagaa acctgcaagc
    12421 accaaattgt ctactttgga gtaataaaca tcaattttaa cgagcgaaca attgggtccg
    12481 tagatgcttg gcgatgtgga gtgtgtaaaa aaagattttg tgaagaaaaa cagcttggaa
    12541 ttgaatcaat tgtggactat gtggggatgc caaaaattga gccagatgaa aaatgggccg
    12601 ttttagtaag aaagttgctt aaaggaaagg acaggtggaa gcttgtcaag ctcaagcaaa
    12661 atggaattct aaaaactgaa acaccggacg acaaggtcat agatcttcca atcaaagagt
    12721 acaaagtcta tgacccaaac catacaagct ttctaatcga tgattacatc aacaaagtaa
    12781 ttgaaatcta ggtgtgtctt ttggacattg aggttcacat caacaacgta aagggcgcgc
    12841 aaatggcggc aaaaattact ggaaccttta ccatacataa taacacgttc aagttttccg
    12901 caattgcatt tggaaggatt ggaggccaca acattggggc aaagatatca aaaacaacgg
    12961 aaaaagcgct agaaaaactg ggctacaatg tagatgaggt aattgacgcc ctacaaaaaa
    13021 gtctcatcca tggaaatatc atacttccaa aagacctaaa aaaagactcg tttgccgacg
    13081 gctaaaactc tgcttgctct agcgccttcg cgcaagaaca attccttact tggggtatct
    13141 cgtctggaat tgacataagg agtttttttg tgttctccac gttttttgag agcgtctcca
    13201 ttacttcttt tgctgttacg ggtttttctg cccagacatc gtaatctgta acagttgaaa
    13261 ttgaggcata gcagatttgc gcctcccttg caagctggca ctctgggacc agcgtcatac
    13321 caataatgtc tgcgccagtt gatctaaaga accgtgactc tgccttcgtt gaaaatcttg
    13381 gaccttctat gcagacatag gtgcagtcct tgtgtactgg tatgctctga gtttttgcag
    13441 cttcaagtat tgcggcctga agctctggac aaaatgggtc agctacagaa atgtggatta
    13501 cctttccctc ctctgaaaaa cttagctttc gggatttggt aaagtcaaga aactgggacg
    13561 gcaaaacaaa ctggcctggg gcaagctcct cctttaagct acctaccgca gatggagcga
    13621 tgattcgctt tacgccaagc tctttgaatg cccatatgtt tgcgcgatag tttatgaggt
    13681 gtgggggtat tgtgtgcttt tttccatgtc ttggcataaa tgcaattttt ttgcccttga
    13741 aaattccaac agtgatcaaa tctgatgttt tgccaaacgg cgtttcaact gttacctccc
    13801 ttgtttcctt tagaaggccg gaatcataaa tgccggttcc tccaaaaatt ccaatctctg
    13861 cttgttccac tagtatttca ccaaagactg acagtggtat tttgctatct tttttattcc
    13921 ctgcagctct gttagctcaa caatgaacgc gaaccctaca accttgccac caagcctttc
    13981 gatcagctct gctgctgcct ttgccgtgcc acctgttgcc aaaagatcgt cgcaaattag
    14041 tattctttgg ccctcactga tggcattttt ttgaatttcc attgtggcct ttccgtattc
    14101 gatgctgtat gatcttttaa cggtggagcc tggaagcttg ccttgctttc ggatcatcac
    14161 cattcccttg ttatatctta gggcaagagc gcacgcaaac ggaaaaccac gagactcgat
    14221 tccggcaaaa aggtctatgt catttgtgtg gtaatgcctt gcgaactcgt ctactacatg
    14281 tgagagtgct gcagggcttt ttaaaattgg actaatgtca cgaaagagaa ttcctttttt
    14341 tggaaagtct ggatattcca caatgatgtt ttgaaggttc acatcgatta cgtcttagcg
    14401 gagtaataaa aagggttcct aacttttact gtacggaaaa tgttgtctct gcaacctcgt
    14461 ttgcaatctt tgtttcaatt ttgtacgttc caggtactgt gtctgggggg actgaccaga
    14521 atgtctggaa gcttccattt tctgtggaga atacgattag ttccgtaatc tccacgtcac
    14581 ttgaatcaaa tatttttatt atgactgttt gtgtctttcc tccaccacta ccagttattg
    14641 tcatagtgtc gccaacactg tatgctgtgg atttgtccac acttacgaca aatgcctggg
    14701 ttactgtgcc tacgacattc aactttacgt ctgcaaggtt ggatccacta cttgccttaa
    14761 ttgtccatat tccttgctct gcatcacttg gaattctaaa cgtaccgtct gaaaacttgc
    14821 cttctttgtc agaaaaagcc tcttttcgtt ttactacatt tccctgcgga tcactcattt
    14881 ctaatacgag tagtacattt ttgttagttg aacccaaaat cagtatgctg tctccaagct
    14941 ggtatgtctg ctttgttacc tgtatggtga tttttccaga accctgttgt aggccaactg
    15001 caaataccgc gttcgtctca gagtgaagat aatttaaaac tatggtgaag acacctgatt
    15061 tgtatccact cagactaatc gaatattctg cacttccatc tgcgcccaaa gtaatggtgt
    15121 ccgcggatcc gggcacgacc ttgtctgagg ggtcaagtat taaaattgag atatttgcct
    15181 ttgcaggacc agttaatact acctttgcat tttcagatgt ggaatagttt agcttgtcaa
    15241 attttgcaac tacttgtttg gttggagcga caccgagtcc aacgtacaaa atttctgtgt
    15301 catcaccctg tgacataatg ataacatagg ttcctttcaa agagatttgc tctgttggaa
    15361 attcaaaatc aattattcca gttccgttca ttttcaaaag atcataaaat atttcctttc
    15421 ctattggatc tttgatcaca atttcgactg attgatctga tgtggtagtg ccattgaacc
    15481 gcatcgtttg gctaggctca tatcttattg cggacggtgt cacgtgtatt gactttgtga
    15541 tggatacact tagtatctta gttattgtat ctgcaccatc gctgaattct actacccttg
    15601 tgccaagagg ggcgtctggc ggaatgactc tttcatgcga ccagtttccc tgcgcatcaa
    15661 cttcaattac tgcttcctgg attttaattc cggtggcatc cttgtagctg attgtgattg
    15721 tggagcctgg ctttgcagta ccgttaacgc gggcagtctc gcctggtcca accatctcag
    15781 tgatctgggt tatggcaaga cgtgccgccg gcttggctgt tatctgcgta tctctatgat
    15841 caagtcgtat gctcaacgtc tttttgtttc cctgctggtc agaaagtaca aactctactc
    15901 tatctgcttc tttgttaact ggaatttttg tccttccaat caagtttccg gagctatcgg
    15961 ttgtaaagtc ttccagtttt tcaccgtcta tgtaaaagtc aactacggta ttgggcggaa
    16021 agctgtttcc aataatcctg atgctgtcac cgttttttgg cgacgctgga attattttaa
    16081 attcggcatt agcaaaactt gtagctggtt tttgtgtatc ttgaggggga gtttgagtac
    16141 tgtcttgagg gggaggttgc tgtcctgcta cggttcttcc agttgtaagc tcgttgccac
    16201 tcaagtccaa ggttctccag tttatgccag gattttgaac ctcagtcttg atgccaaact
    16261 ttacagaatc gccaggcggc agtggttctt ctgatgtgaa gattagtagt ccctggacag
    16321 atagggcgcc agtccagccc ttttctgttt taaaagactt gaaagtaccg gcgtcttggc
    16381 caagccacat ccttacggtc tttattggaa ctccctcatt attgacatat tcaatcaaag
    16441 tagtcttttc aaagcttgta ctttttgcaa cggtatcggc aaaaacaggc gagcttagac
    16501 tggcaacagt aaacactgac aaaataataa ttccaaacag tgctagtctt gggtgggtca
    16561 caattaatct acctactttg atgaacatta tttatttacc ctttttgagg gttggggatt
    16621 attcataagt aaaatacttt aacgacttag tgtgacttgc ttctttggta tttgtttaat
    16681 cccacctgcg ccatgagatt ttgatgttgc ctaattcttt ccgcaattag cctgtataat
    16741 cccctgaact ggtcctttgt tttatcagac aaaaagtcgg cttcttttag agctattttt
    16801 tcgcagatgt atcgagatat ttcctcaatg tcaaactcgt tccaaccatc gtctctgtgt
    16861 tcggcccaga tgctgcgcag gttttccaca atgccttttt tgctttttgc aagaacctct
    16921 tcttttgtga ggtttcgaaa accctccttt cttagcttga tctcataggt ttggtttact
    16981 gcgtgcaaat aatcgtctgg gactattagg tcttcgatgg attctattgc ttttccactt
    17041 tgctcaagat aaattatctg catgtctgta aactcgtttg tctttaggtg ttctgcaatt
    17101 ttttttgatt cctgggtgtt atcaagtatg acaaagacgt tgtggccgtg gtttcggtaa
    17161 aatatcgcaa ggggtaaagc cgagtcctta ctaaatgctg caacaacgtt tagtgggttc
    17221 atggatatgt ttggatcctc taggaacttg tcaaaggcgt taaggtacat tgcgtcagcc
    17281 atcgtctcta caattatgac tggccttgag ttggtgccaa tctctctgtc tactattgac
    17341 tcgacgaggc ctcttgacaa accatacaaa attggaataa gcgttgcatc gtcagcattc
    17401 caataatcat agtagatgcg gcttaggtgt cgtcgcttgt caagctcaac tactagtagg
    17461 tttcctggag tataatcaaa tatcataaac ggtgagtgtg ttgcataaag cacttggttt
    17521 gatcctgcca agtttttgag aagatcagag attcccattt gttgcgcagg atgcaggttt
    17581 cgcgccggct catcaagaag caagattgcc tcttttagct cagcacgttg cgtctctgcg
    17641 gcaaaattta caataaatga aaatgtccac ttgaatcctt ctgctcttct gctaagaaga
    17701 ccagtgttag ttacagtccc atccttgtgt acatcagata ttactacact cattacattg
    17761 cctgggttgt atctgaggtc aacatgtatt gggtccccct tccatgctgg gttcagcctg
    17821 tctgtgagcc tcctgcttgc ggcgtttagc agtttgataa gctttggcgg gctgcgcttg
    17881 tattcttcaa gtttttctgt gtcaagctct gctagataaa acaggtttct tacggtttct
    17941 actttgtcaa actcttctga gtaggtaaga tcttcgggcc gctttatctc ctttatgtac
    18001 tcgtccaagt ttatgtttcc gtaaattttc ttgtaatctg aaaaatacac gaaccttggg
    18061 tgcaagtgtt ccaaaataaa gttctcaagt gcagtttttt cggtggttcc aactaggagc
    18121 tcgttgtatg tcttttcggc gtcctggtaa tacccagacc actctgttag gatttgtggt
    18181 tcgtctgcag ctagtacatt taggatgttg ctgaactctg tcatctctct aagatacgtc
    18241 tccttgtctg gtggagggcc ctcaaagaac ttggtgtcaa actggattct tatgtggttt
    18301 ggaatagttg agataaaatc aagaatcttt tcagtataat tttcccacga gttcaatgtc
    18361 ttgtttaact cgtcgcttag ctttactgag ccaaagtcgt actggacatc tactatcttg
    18421 ttggtcctaa agatcttcag ggctctaatc tctggcaggt gtgggaatct ttcttttatg
    18481 aggctagtct catttgagct gagggcaaac tcgccttcaa caagtgtgac ttctgatttg
    18541 agttcctctg tcatctcgtc gcaaaggtca agctcagaga ccagttcttc tttgttgagt
    18601 agcgttagtg cctgcaaaat tgtggtcttg ccgctctcgt ttcttcctac aaaggctgcc
    18661 aaatcgccta ctttgatttc gccagagtcc tggatgcatc tgtatgctct gactctgaat
    18721 tttcttaatc tcatctagcg cctatttgct tggcagcgat ttaacttatt cgacacatta
    18781 ttacgctcaa ttttcgctaa aagatataaa gatacagaac accttagaaa aaatattggc
    18841 agcttccggt gtaaaaattg cagtatttgt aattccaatt gtgttttctt tcatctatgg
    18901 aggtgcagta ctctcactgt cactgcagga gccaaaccga ggaattgact ttcaaggaac
    18961 tactcaatta acctcgattg aaattacttc attacaggat gaatattcta gttcggatgc
    19021 aatcactgcc caagttttgg tacgtgatcc cggatttagc tgcggagatc tctacatgac
    19081 gatctacgat gtatcatcag ctcaaagaaa ggcagttgca caaggcgcgt tttttgatca
    19141 atgctatgat tctagtggta cacttccagt aggtgaccag ttttcacaaa aagttgatgc
    19201 tgtaggacag tatttgcttg aagtacagat gtttgaccaa agcggcgaca agtttctaac
    19261 aacaagccaa aaattcagtg taagatagca tcttaatgcg atattatata ttgaaaccaa
    19321 ctatgagtac ctgataagta cacgtgagca aaatgggaag gccaaaaaaa tctgtaattg
    19381 attctaccga aataaaaaaa caagacaaga aaaaagaaaa gaaaacatca aaggtagaaa
    19441 agaaaacaca aaaagaagac aaaaaaattc tgccaaagga agacaagaaa gacaagaaaa
    19501 aagacaaaaa gatagcaaaa gaagacaaga aaaaagaaaa aaaggccaaa gaagagtcgg
    19561 aaaagacact agaagaggaa ctagaagaac aactcaccga cgaagaaatt gaaaactttc
    19621 agatcgaaaa ggtcgacatg gataagctca caaatcgcgt gtgtttgttt ttagcaaatt
    19681 atgaggacgg tattgttcaa agcgagcttt ggaaaaaatt caagcttaca agcagggacg
    19741 gctcgcgtct tgcgctaaag cttgagagga tgggaataat cacacgtgag aaaatactag
    19801 aaaacaaaag atggacctat gttctcaaaa taaagaaaac accagttagc actgcatcca
    19861 ttgaaaatgc tccatgcctt gtttgtccag ttgagcaaaa atgtagcctt gatggtgaag
    19921 taagtccaag aacctgtcag tggattgagg actggacaat tattgaactc tcaaaaccaa
    19981 agaaaaagaa agaacaatga gactagtaga tgcaaaacgt gatcattatc ggaagcttgc
    20041 gcgtgaacgt gggtatagaa gcagggctac ctacaaacta ctagagctaa acaaatcata
    20101 ccgaataatc ggacctgggt tttatgttgt agatttgggg tgtgcgccag ggggctggac
    20161 acaagtggca gtaaggcttg cgggaaaccg cggcaaagta gtcggcgtcg acactgcgtt
    20221 tgtagaagaa attgaagggg catacatcct caaagggagt gtggaggatg aatctgtagc
    20281 agatgaaata ttagaatacc ttgaaactaa ggcaaatgcg gtaatatgtg acatatctcc
    20341 aaacattact ggccactggt caatggatca tgcaaaacaa atttcgttaa actattcctg
    20401 ctcaaaaatc atggacaaga tacttgcaag caaaggaaac gcgctcttta aggtatttga
    20461 tggagaatat tcaatagaat ttagggacta tttgcaaaaa aaattctcgc gcgtgcacct
    20521 aagaaaacca aacgcaagca gaaagccaag cagcgaaatg tactacattt gccttggata
    20581 caggggctag tgtgcaagca attctcgtat cttgcttatt ttacagtcgg ttctaaatcc
    20641 agtcggatta aacgatgtgg ggctggacac aaatccattt tcttgcagac gcttgattga
    20701 ttcttcaagt gaaattggcg aagacttgtt catttcagca atctcatcaa gggtgtagta
    20761 acatgcaggc atattggatt caagtacgca tttttgtatg attttctcac attttttgtc
    20821 cactgagaaa ttgggagtct cgctttgcat cgattctacg aactcttttt caaagagctg
    20881 gccaatccac agcggcccgg caatctctac tttggagcca caaaactcgc agcatagtgg
    20941 aggttcacgt gtggcgcacc tgttcccaca agccctgcaa tgtgcaatga acccaattgt
    21001 gtttcgtgta tctggtttgt ttaggactct tacatagacg cgataataat gctggttgtt
    21061 ttcaacaaag attggagtta tctctgcatc tagtcttgct gcaatcatgt tgatgcagcc
    21121 aagaatgagc ctaattgaaa tctcattgga atatgtggtc ttaattggtg tgccgtgata
    21181 cttttttttg cacgcatctt tgaacaatcc gtgcaagacc tgaagatctg ttgcagtaac
    21241 cgacaagagc cccttgtgaa ttgttgcgcg tattgcacag tcaatgtgtt ttactggcga
    21301 gccaaagggg tcgacatcga ctattgctcc gcgcctgccc ttttctgaaa aactgctcaa
    21361 aaacctgcaa atctcatttt ctgaaacctg gtaattttca agattgttga gggcagcaga
    21421 tttttttgac agctcaagcg cactgggatt aaggtcattt acaaaaactt gaatgccaag
    21481 ctcattcgca actcgcaggc cgcgtgcacc aagacctgat agaccatcaa gaaaagtttt
    21541 tggaccctca aagtttttca gaaatgccga ataggcaata atggaaaggt ccctgctcag
    21601 cttcgcacgc ggattaaaaa atgctggatc gcgcggcgga actttgtttt tcagagaatc
    21661 ctgcggaatg agtatctgtg tctttccctc aattattgtg acgatttggt cattgagttg
    21721 catactggta gttagttttt gtacgtctct aacggtttaa tacgttaggc cattgtggta
    21781 ggtgtgatta tttgcggcgt agatgaggca ggaaggggtc caatgattgg gccactcgtc
    21841 gtagccggaa tagcaataga aaaatcaaag ataaaaaagc tggctgagat tggcgtacgc
    21901 gattctaaga agctaactcc actggcaaga gaaaaaatgt acaaacaaat cacaaggatt
    21961 gttgatgact atgcagtatc aaaaataact ccaaaacaga ttgatgtcca tgttctaaaa
    22021 caccagctaa atcatcttga ggcaatacag atggcaaaga taatcagaaa actacaacca
    22081 tcggtgtcat atgttgattc gtgtgatgtg aatgcgacga ggtttggcca agtgctagca
    22141 aaacttgcaa aaactggaaa aatcaaatcg taccatcgcg cagacagcag gtttttggta
    22201 gtttctgccg catccataat tgccaaggtg actagagaca gggcaattgc aaaactcaac
    22261 gagagctttg agattgggag cggatatccg tctgatgcaa aatctgttca atttgtcaga
    22321 gagtggtttt gcaaacacgg gcagatgcct gattttattc gcaaaagctg ggcacctgta
    22381 aagctagttg taaagaatta tgccttggcg tgatatatcg ctacgaccat ggcgtggtcc
    22441 ttgtcatatg gctgcaggtc catgttctga atgactgaga aactctggtg cagttttttt
    22501 atctcatttt caattatttt ctttggcgac tgagttacat cgatgctccg tgttttgata
    22561 gtcataaaca aataaccacc gtcctttagg aacatttcgc agttcgttat tgctatctgt
    22621 gtttggtctg gctgcgcaat gtcagaatac acaacatcga ctttgccgta aattgaaaag
    22681 tattgctttg gatgtcttgc atcctgcaaa attggaatga tattttttct aaaatttgca
    22741 acacggtcca aaaagtcccg cgcgaccctg cttgtgtgct ctactgcaaa aatcattcca
    22801 ttgtgtccga caatgtctga gatgtggctt acagtcgtgc cagtcgaggc gccaaggtac
    22861 agtatcttgc ttccttgctt tatcggaaat acgtctagtc caatcattag cgctgctgca
    22921 agcttgctcc taaatggatc ccacatccgg tactcgactc cgcctttttt gtataatttt
    22981 tcgccgtaga cctggtttcc ctcaacatag tttatggtgg caagtctttt ttggccatcc
    23041 actgtcagcc aaaagatttg attatcttct ttttccaaac ttttttcgct tttttagatc
    23101 cgctctaaaa tctcttcggg gacctctgtg cctaaagttg tccctttgtt tttcaattct
    23161 ttgtggcgga attggtcggt gtgatggctc tttgtatttt tgtccaattt cagttacccg
    23221 gacatttagc ttttcaagca aggtgtggtt gatgcctcct ccatacacat caactctggc
    23281 tgctatggcg gcctttgatg caattgcccg tgccatcttt cctcgctgcc attttggtga
    23341 agcgtgaaca actgggtgtt ggaacaggat tccgtgcttt ggtggctgcg ttccagtctt
    23401 tagcgaccta aagagcgcct tttctgcacc taggatctgg atggtacttg caggcataca
    23461 tgctaacttt ttgaggcttc ctgccttggc aagaatcctt gcaccaactg cggtccccaa
    23521 aattatcgcc aggtttggtg caatcgtttt catttcgttt tcaatgtgag actcaatctc
    23581 ccttctaatt tgtgccatct caaggatctg ctttgcaatt gactggacaa ttaccaagtt
    23641 ttgatccgag atgtcgcctc ccttactttt gtcttgaatg acagataaca tttctacttt
    23701 ggattctgga aagcctgcgt cctcgtacac ctgctttgag agcatatcgc gctttccagt
    23761 catgacaatc tgcgcatatc cgttgatgct atcgataatg ttgtcgagct ctggaaaatg
    23821 aagaccgtac cactctcgaa gccttgatga gagaccgttt accatgacgt caatttcgtc
    23881 tagtgccttt atggactgga ttatgtgcaa gtctgggctt tgcgagacct cagttacctt
    23941 tgcagccgaa agctgcagcg caaagtcgcg cagtttgctc attgcgtcag cctggtcttt
    24001 tgcaaacccg cagtcaatta ggatttgggg cttgtttgtt tggatctctt ccatctgttt
    24061 ttcatccatt agctgcacat cgagagattc ttttttgaga gtctttagga gcgattcgtc
    24121 acttaacaaa aagacgctat tggtccttct tagaaaatca atcagagagc caagcctgag
    24181 ctggccttcc tttactttta caaaatcaga tgccggttgt gcaaaggcaa acgagttgac
    24241 cattttgcca ttctccaaaa ccacaatgcc aagttctgtt agaactacaa aatacataca
    24301 gcattctctc attccctcat taaaaaagct aacaatcgca attcataaat caaacattat
    24361 aagactacaa aatcaactca atcaagtgaa tccagacctt tctgtcttga ttggatcaat
    24421 aagcctcaag cggccagcaa tgttggcttc aggaatttta ggcatctccc ttgaagtctt
    24481 tgaaagaatt cacaagagtg gtgccggagc agtagtgaca aaatcgctaa gcaaggagcc
    24541 gtgggacgga tatccgaatc cgaccatagt aggaatcaaa ggcggatatc ttaacgcagt
    24601 cggcctctca aatcccggcg ccccgtactt tgcaaaaatg atttcaaaaa atacggacgt
    24661 tccaataatt gtgagtcttg ttggttctct agaagacgaa tttgaattca tggtaaaaca
    24721 gtttgaggga atcaaagttg ttgcatatga gctgaatctc tcatgtccgc acgtagacaa
    24781 agtcggactc gaagtcggtg atgatccaaa cttggtcaca aaaattgtaa agagaataaa
    24841 atcaacaacc aaagtccccg taattgccaa agtggggctt ggcactacta actatcttga
    24901 tacggtggca gcagcaatag atgggggagc agacgcaatt actgccatca acacaattag
    24961 ggcaatggga atagatgtgg aaacacaaag gccgcttttg agccacaaga ttggcgggct
    25021 gtcaggtact gcaataaagc cagtggcagt aaggtgcgtc tatgaaatag catcagaata
    25081 tgacattcca gtgattgggt gcggcggggt gtcgggctgg gacgatgcag tcgagttcat
    25141 cttagctgga gcatcggcag tacaggtggg cagtgcaatt ggtgatcggt ggattgatgt
    25201 gttttctgaa attaacgacg gaattgcaaa ttacatgaaa aaaaaggggt tttcaaaaat
    25261 ccaagagatg gtggggcttg caaaacgctt ctaatactcc gtcgattaga attattgaaa
    25321 aggtaatcga tgaaacaccg acagtaagga cgatgatagt ttcagatgaa aaattagccg
    25381 acgtgttgcc tgggcagttt gcaatggtct ggattccggg tgtaaatgag cttccaatga
    25441 gtgtgatgat aacagaacaa aaaggaaaag cagcgtttac cgttcgaaag cgcggcgagt
    25501 cgtctactgc catgtatgat ttgaaggaag ggcagcaaat aggcgttcga ggcccatatg
    25561 gaaattcttt tgacatcaag gacggcagga ttttgcttgt gggagggggg acaggacttg
    25621 ttccgcttat gcggcttgcc aagtttgcaa aaccaaacaa cgacgtgacg attttgatag
    25681 gctctaaaac aaaagaagag gtcttctttg aggaactcgc aaagaggatt ttagcaaaaa
    25741 ataaacacaa aataattgtt gcaacagagg atggatctta cggggagcaa ggattcgtca
    25801 cagatgtttt ggaaagacta gtacagaacg aaaagtttga tgcaatctat acgtgcgggc
    25861 cggaaataat gatgtacaaa atagttcagc ttgcaaacac aaaaaaaatc tttgtgcagg
    25921 caagccttga gagaatgatg aagtgcggaa taggaatctg tggcagctgc tgcatggacc
    25981 aagtgcttgt atgccatgac ggaactgtct ttgatggcga gtttttgaaa aactcaaagg
    26041 agtttgggca cacacataga agcaaagcgg gaatttttga gaattactaa agtgccacaa
    26101 agtaccatcc tacgggcgta aaaataccta aaggtttaaa ataaataaaa taagagacgc
    26161 gacaggagaa aattggcaca cccaaagatc gtacttactg cagatcgtac actaatgtca
    26221 aattatcgtg gtatatcgct tgcaacattt tttggctgtg cgcctgcact tgacccacac
    26281 agggatcata acagcttttg gtacaagatt ctaaaaaacc aagtgactcc aaaggttctc
    26341 tttgatttta tatgcaacac ggttcctcac acaaacggcg ttgcaaacta tgcgccgtac
    26401 ggcctccgaa aggtggaagc cgggcttttg cgcgacggct tttcgcgtca ggacgtggtt
    26461 gtagcacatc cagatcacgt tgacaagttt attggacctg agaccgaagt cgtcggaaca
    26521 tacgagatgg atccacttgg catgggccca gtcaccatga catttaccta tggaagaaag
    26581 cagatctcat acgatgaata ttacaacatt tatcttcaca aaaaaattaa cgaggccaaa
    26641 aagaaaaacg gcagccacgc caaagtgatt tccggggcgt ctggaacctg gcagtacaac
    26701 tatgacccag aaaagattga ggaattgggc ctgtacgcca tactagaagg ggagcttggg
    26761 gggattgcgc cagaaatcga cgggcatgcg ggcagatttt tcaattatct gattaacggc
    26821 gaatttgaca acatgaaccc gtttagaaaa agaagcgact ttaaagttga catcaaagag
    26881 taccagcgcg gggatcgtac cattcacgga agatttgtga acttttggga tcggccagaa
    26941 atagacgaga ttccagatat agtcgagcca tcaatgcacg ggatggttga ggtgatgcgc
    27001 ggatgcggac ggggctgcaa gttctgcgac gttacactga gggcactgag gtactatcct
    27061 ccagaaaaag tcaaaaagga aatcgaagta aatatcaaaa aaggcggact taaaaacgcc
    27121 tggattcaca gcgacgacat ttttgtctat gggatggatc cgagaacaag caagcagatg
    27181 gagccaaaca gggaggcact agaggaattg ttcacagcag taatgtcaac tggtatagtg
    27241 cacaccaatc cgacacacgg aacacttgct ggcgcaattg cagacgaaaa actaattcca
    27301 aacatttcaa agataataaa ggctggcccg gcaaacctaa ttggaatcca gtgcggattt
    27361 gagactggaa gccttcgact gattggaaaa tatgcagaca gaaagcttgc accatttatg
    27421 ccagaggaat ggcactgggt agtaaaagaa ggcgtaaaga cactaaacga gaactattgg
    27481 gttccggcat ttacgctaat catgggacta gataacgacg agacacctga ggactcgtgg
    27541 gagacaatcc aactcataag tgaactagag cgggagcagc cagaggcgat gtttacaaca
    27601 acaccactca catttgtccc aatcggactg cttgaaaaat cggaattctt taacattggc
    27661 aacgagatga gccctgccca gcttggcgta atgtataaga cgtggcagca caacttcaag
    27721 tacggaatcc agaaattcat gacaaagact ggtgctagtc agactgccat gcaaagaaag
    27781 ttctttaacg gacttgcacg ctctcttggc ggcgtgccgc tttctgcaat ggagaaattc
    27841 gcaagaagaa agagcaaaga gcacgaacaa gtcatcgaga aaatcaaagt caagtactgg
    27901 taaaccccta gtaaagactc ataagtgcaa ttctaagact caatacacat ggcaactcac
    27961 ggatcgatta caaaggcagg aaaggtaaag ggccaaacac caaaagttga aggacgaaag
    28021 cgaataggaa caatcgctat tttgcgaaac aagagcaact ttaggaaaag attctcacta
    28081 cacaggactc ctggtcaaaa caagcccggc caaagaaaga gaagacgata aacacacttt
    28141 tttattcatt ttgtaggctc ggatcatcaa atttctaagc aaacctaatt tacttggggg
    28201 tacatagata ctgcggcaca gaattgacat gccgtttttt agaacggaaa atgaacaaag
    28261 gcttgggacg tgcgaacccc cgggcttttg ctcattgatt tattctgtgt ttgtttgatt
    28321 cgaataaact atctcataga ttctctacca gattaacaaa aatggaatat gggactaggt
    28381 atagcgatag ctggtggaat catttgcgca actgtacttg cggtattctc tattgttttt
    28441 tctactagtg ggcagatcta cgaaatcaat tcgtctagga cttctgctgc agacctgcag
    28501 agtgtagttt ttcagacaga catggcagtt agtgatctca cggcccaatc aggaagccag
    28561 tttgttaatt tcaccctgac taatactgga aacgaaaaac tttggaatta cgaaaaattt
    28621 gatgtcatag taaattatac ggcagatgta ctaggttttc ccacccaaag aatagagagc
    28681 attacctata cgacaaactc tgatgacatc tcaattgaag cagtttcaag taattcacaa
    28741 acttgtattt catgtaccct atcacatacc gttgctggga caaacaagct gctggttgtg
    28801 ggcatatcaa tatcggacga tacggctgtg agctcgatga catattcggg ccaaagcctt
    28861 actctgattc gctttgacga agttttatca actgataggc gctctgagct atggtatcta
    28921 gttgatcctc cttctgggac agctgatgta gtggtgacac tggatcaagt tgagagagcg
    28981 attatcgggg caatctcgtt taccaacgtt gatcagtcaa atccaattaa tgtcgataat
    29041 ggtgctattg attcggtggg ggcgactcat ccttcggtaa gtctaacgac tactgtagat
    29101 aataccttga ttgttgatgt ggtcagcacg gtacaggggt caatgacgcc tagcacatct
    29161 caaacagaac gatgggactt ggttcgagcg cagctggctg gcagtggaag cacaaaccag
    29221 acaactacgg caaatactta cacgatgagc tggacaaacg aggggggact ggatcagtgg
    29281 gcaatgagtg ctgcggctat acggcctgca gacccagcat gtggtccaga cggaaccatc
    29341 gcgacaaaca actggctact aaacagcatt tcaaatgatt atgcagatcc aaatctggtt
    29401 aacacaaatg aaagcgcgtt gatatgcaca agtcttgcgt atccagttta tgccaacgga
    29461 gatgtgaaag ttaccgtttc aacagacaat ggctatacca aaagcagttc tacaacagca
    29521 acctagtctg taaaacaaaa tttcgaatca gataattcta gtggaatccg ccccaaacta
    29581 gaaatcaatt taatgtaaat catttccaaa tctggaaatc ttacaagtct tttgtatcac
    29641 ttgtgcaaaa attttgatca agtttagaat aaaccttatc acgcataatg tatgaagata
    29701 agaggtatac cttgaaaaaa tgtgttgtgt gtggaaagga gtttgacgga atagagcgcg
    29761 atgtgtgctc ttggcagtgc gatgaaaatc gtcttgcccg actcagaaaa aaactagatg
    29821 atgcggtaaa aaacgataaa gggcatacaa aaaagttgag cgaagattct aacgaaacga
    29881 caaatagata atttaagttg catcctttga aggtcagctg ttagctgatc aattacattt
    29941 tatcgaaaac cgatcttaga taatccatgt catatgtatg cgatgaatgc aaaatcatgt
    30001 tttttcgcaa gatggtcttg atgctcacaa ccaaacaaaa cacagaaaaa ttgaagaaaa
    30061 agacgacaaa gttttggatt cgtttgtatg agtacaaatg agcacaatcg ttttaaaaaa
    30121 atacaattgt ctgcagcaat ttcaaacaac ttaaagagca cgacctgata attttatcag
    30181 attcgtgcga aatgaaacat agaaaatggt cttgaccgga gcttgggatc gtctgctgtt
    30241 ctttttctta ggctctggcc ttcattacca atatagtaac aagcggatcg tagagcataa
    30301 caaagtcaaa aggggcccgg tattagaacc agtcataaac aacacgattt catttttgaa
    30361 aaccggctga gcgattaaat accttaaagc tctagtgatt ccataactaa atgagcgaca
    30421 atctactttt ggtgaaattc cggtttgacg gcgaaacaaa atcccaagtt ggcaagttca
    30481 cctatgaaca atatgataat ctacgttccc ttcctattac ggtagagtgc aaaattatct
    30541 ctaatgaaaa acccaccctg agtaaggagg agattgatat cattaatcaa aaggtaattg
    30601 acgcttggaa ggcagacagg agccatacct caaaacttgc aaaataacta gttcgtaaga
    30661 ataacaaaaa ctacaagaaa aagtataaag aatgcatacc agaagatgaa ttaggctatg
    30721 acattagcct tggtttaacc acctaaaccg agtctgtgac tctgataatg tctactagag
    30781 gagaaaatgg taggcatgaa aataatagaa cttgcatttt tggcgatagg tggagtcgca
    30841 gggacattcc taagatacaa gattttgaca gccccatact acatcggggc gctgcaagcc
    30901 aacgtgttga ttgtaaatgt aattggcagc ttcattcttg ggatttttgc ggttttatca
    30961 caacaatgga accttgatgg gaaatatgcg ctgcttgtcg cgtttgggtt ctgtgggtca
    31021 cttactacga tgtctgcact ggcctatgac gcccatgatc tgttaaacaa cgccaagctc
    31081 ggtcttatgg cagtaaacat tcttgcaaac gttggcttgt ctgtggcagc catatttggc
    31141 ggaaaagctt tgttcaactt gatactggga aacgatccac tgcaatgatt gattgatcca
    31201 cctgaaaaat gagtactaga atcgaaggcc agaataaacg gttcacccta attcaaaaca
    31261 cctcagcaaa aaacacttaa attttataaa aacttggtca aaacagtgtc tacaaactat
    31321 ttgccataaa cagtagtatt ttggattctt tgcgacacta gattttgtaa tttttcaatc
    31381 attgtcttgt tcgtaaacat cctcaagtag accaattgat gtgatatgga aaaaaagaaa
    31441 aacacagcaa aaattgtgga cagatcaagt gttaactata cttttaaaaa taacaatcca
    31501 atctatcttc aaccaaaaga caagcagaat gatgaattaa accaaaaaaa gtcacagaat
    31561 cctaccaaaa gagcaattaa ggatgtaaaa agtcacaatc gccacaaagt aaatagtaaa
    31621 aagatcaagt cattacaagg ccattcacca gtctacgaat aagatcaact aaagaggcaa
    31681 caattctcac tacaataacc ttataaagta ctagtacggt accttacggt atggtagacg
    31741 acataagatt agacagtctt gatggcgtag ggccagttac gacaaaaaaa ctaagcgatg
    31801 cgggaattca caacatttac gatctggtta tcaggggtcc agtagatgta gccgaagtaa
    31861 ctggcatgga cagggacaca gccgataaaa ttgtgaccaa ggcgcgacac cttttgatgg
    31921 aacagggctt gatcacaaag gactttgtta ctgcaactga aatttacaaa agaagactag
    31981 acataggcaa gatcacaacc ggaacgcaat gtttagatga cttgcttgat ggcggtctgg
    32041 aaacccaggc actaaccgaa gtttatggcg agttcggttc tggaaagaca caattttgcc
    32101 tcacaatgtg tgtgatggca caaaaaacta aagaagaagg cggccttgat ggcggagtct
    32161 tgtacattga cacagaaaac accttcagac cagaaagaat cgtatcaatt gcaaaagcaa
    32221 agggaatgga accggaaaag gttctagata acatcattgt ggcaagggcg tacaacagtg
    32281 ctcaccagac actaatttta gaagaggcag gtccaataat tgagcagcac aaaataaagc
    32341 taattattgc agattctgca gtggggctgt tcaggtctga atacctaggg cgtgggacac
    32401 tgtctgaaag acaacaaaga ctaaacagat ttgtacatct tttggttcga actgcagaga
    32461 catacaactg tgcagcaatt gcaacaaacc aagtcatggc atccccagac gtattctttg
    32521 gcgacccgac aagaccagtt ggtggaaatg tcgtagcaca tactagcaca tacaggattt
    32581 actttaagaa atccggcaaa aagagaatcg ccagaatggt cgacagtcct caccacccag
    32641 aagaagaggt aatctttact gtaactgagg gcggggttgc ggatcctgaa gacgacacca
    32701 aaaagaagaa aaaatctgaa gaatccgaat agtcacgacg atgtttagac atctatggag
    32761 cttagattcg gcccaagatc ttctttttct ttgactgaaa ttcgctttca gtaattattc
    32821 cggcctttct caactcgcca agttttgcaa gggtttctag atccgcttta tcagttgatg
    32881 caagtctctt tgcagtcgat atacctttga tgatttcctg ctctcctttt gactttagtt
    32941 ttgcaccttc aatctttgcc tgttttctaa gatctctgct ctttctgtca gcttcctttg
    33001 ctgcaatctt tccaaactcc actgcatctg ccaaaatctc gtcggctcgt tttactcctt
    33061 gggttaatgc ttcatctact gccttgtctg cccttttgag aaatttttca atatatcctg
    33121 gctgtttacg ttttgccata ctctataatt tcatcttcta gtattattat ttttgatgtt
    33181 ttggctagac tttaatagaa atggcatgta gaataagatg aattttggtc aacaaggatt
    33241 acaagagcca agtgattgtg gttcgaaaaa aaatcgacga gggcaaaaca agacaaatgg
    33301 tggaagaaaa aaagacaagc ctgtttcgct cacttctcaa aaaacctgcc aaagacgaag
    33361 tgcacataca ctctattggt cttaactatg aagcaatttt gatgatttct ggcagatatg
    33421 ttgccgattt ttatcgcaag gcaacacacc caatcaaagt ggattacaac gtaacacaag
    33481 ttgttctggg ggacggcgtc tttccaatcc aaataaaatc tcgctggaaa acagcgcttg
    33541 gcggaaaaaa aggaaaaaac aaagttgact tggagctaga agaacacgtt tccattgatg
    33601 atgagtgcac actttatttt gatcaccatg gccggcaaac caaacttcca ctcaaactgg
    33661 atcctaaaaa catggaaaac tatccaacaa aaatactgca aaacacaaaa ttcatagtaa
    33721 aaaaaccaga gcttacccac aaagatgcaa ttagtaaact tgaggaggaa ctcaaaaagc
    33781 caatagggac caacgcccgg aatcttaacg aaaaaattac aataactgaa atctcggaaa
    33841 tctatatccc gatttatgag gctaggctgg ttgggccaaa aaagaaagta gaaattttaa
    33901 gaattgacgc agctaaaaac aagattcttt gagtcttttt tgtaagtctt aaaatatggg
    33961 tttaaaaccc aacttttcat gaccactaaa aagccatccg gccactctca cggttttcga
    34021 cacaagtcaa gatcagccct tacaaaggat gcgccccgcg gagtatcgtt tttactaaga
    34081 gagtaccatg tcggagaaca ggcactagta atcatagatc caagacagca caagtccctc
    34141 ccgcacagac ggtaccacgg aaaggtgggt acaatcacct cggtgggcag aagagccgta
    34201 acaatggatg taaaactggg taacaagacg aaaaccttaa tcactagatt agaccatatc
    34261 aaaccgtttg gggtaaagta agatggaaca agtaaagaaa agccagccaa tatcactggc
    34321 cgaagtaaag gacattttgg gaaaagagga tgttgaaaaa atggatcaaa ttcagcgctg
    34381 gacttatgat tatgttacca aatttaccaa agttgacgct aaagccgcaa aaaaaataaa
    34441 acaggagcta aaaaaggatt gtggcctcac agatgaggaa gcaactgaaa tagtgaacat
    34501 aatgccaagc tcgcttgcag agctcagagc gttcaccttc ggatggaaga agctgattct
    34561 agcagaaaca ctagaaaaaa ttctacatat acttaagggg aactcctaga ataggcaagt
    34621 ggttttcttg gaaaggagca ccagcccacc acgtaagtat gaagagtatg catacgtact
    34681 agactatgtt acgcgcggca aatcaacaac agtcagagga cgcgagggaa taatcataac
    34741 tgcaataggg gaggacaggc tgacactcct agaggtgctt ggggtaccaa acgccgcatt
    34801 tgagataggc gagcgaatct atattggaaa ggaaggccgc actaagatct tatcagtact
    34861 tggcaagctg gaatacgaga ggatctcagc agcagcccaa tctgagctca aaaccgtagt
    34921 gcaaacgatt gtgacaaaca atgaaaaaag attcatagat tacatcaacc actcgcagcc
    34981 gctgacgccg agaattcacg cactggagct cataccggga atcggcaaga cctacatgaa
    35041 gacaatactt gaggagcgag aacgacagcc atttacggac tttggcgact tgcaaaaccg
    35101 cgtcggacta aaagagcctg caaagcagat aacgcagaga ataatggaag agataacagg
    35161 cgaagtaaag gtaacactct ttgtcaaaaa atgaataaac ggcaaaggct tgggcaacac
    35221 tttctcaaat ctgtaaacat tgcaaaactc atagtcgagt ccgctcagct aacaaaaaag
    35281 gatgtggtgc ttgaggtggg aacaggaagg ggaattctga ccccgttgct tgccagaaag
    35341 gcaaagtctg ttgtctctgt cgaatcagat gagcagctat attctgatgc catcttgcag
    35401 ttttcttaca tcaaaaacct tgagctgatg tatggtgacg gctttgagac ggattctgat
    35461 tttacggtat ttgtatcaaa cctgccgtat tctgagagca ggcatgcaat agaatggctt
    35521 gcgcaaaaaa agttctcaag ggccgtaatc atggtccaaa aagagtttgc agacaagctt
    35581 gcctcgatgc aaaaaagaca gatgcgtgca atctctgtta ttgcaaatta tgcctttgag
    35641 attgaaaaaa tcatggcagt aaataagaac aactttgagc cgcccccaaa ggtggactct
    35701 gtgatactgc gcctaaggca gaaaaaacaa gtcacgccag agctaatcga gtccgtaaac
    35761 ggcctttttt cataccggcg caagaccata tcaaacattg caaaaaagtt cggcaaaaaa
    35821 atacaatcgg ataaaaggct agaggatttg agcggagatg agataataaa acttgcaaaa
    35881 caaatccgct gagacctaca ctcctgcaga ggatacgttt tttttggcag aacatatcgc
    35941 aaaagaaaag ggcgagtctg ccttggaaat aggtacgagc tctggttatc tgtctggggt
    36001 tttgcagaaa aattttgatt ttgtagtatc tacggacatt gattttggcg tactaagcgg
    36061 ccagaaagga aaaactcaaa acaaaatatg ctgcgatggc gcaaatgcgc tgggcaacaa
    36121 gtttgatctt gtggtttgca atcttccata tttgccatca gaaaaaataa ttgacaaaac
    36181 tacagacggt ggtcctgagg ggctactagt tccacttggc ataataaagt cggcaagcat
    36241 ctgtataaag tctggaggaa aggcactttt tcttacatcg tctttggcaa actatcaaaa
    36301 actaattgaa aaagcccagt ccttgggatt ttcagtcaca attacagcaa aaaagaagct
    36361 gttttttgaa gaattgataa taatcgagat gaggaactaa gtggtaaggg tggcacggca
    36421 acaggggcat ctccaatact ttgaaacaag atgcagcttg caaaaataac accattttct
    36481 aaatgttgta tggtttgcat gtcttggcga ttccgcatta aaataagaac aaatttttct
    36541 gcaatctctt tccaagtcag atcatcctct tctttaccct tgccttggtt cgaagcttga
    36601 tgttacaaca agggcagcgt attgtgtctg acacaaggaa aatggagcat acggagcacc
    36661 atttctgccc ttccccatac ttggagtgtg tagttacttg tcttgccctg tatctttgac
    36721 agatatcgtt acagctttgc atcaaaaaac cacctcaaaa aaaatggagt ttatttctcc
    36781 agaacagatc tgagcatcca tgcagtcttt tcatgttttt ctagcagtcc tgtgagaaag
    36841 tctgcggttc caacatcact gtgttttaca catgactcca catctgcacg cagggtgcga
    36901 attatggatt cgtggtctgc tacgaggttt gataccattg cctgcgcatc tgggtatgag
    36961 ccgggatgtt cctctattga tgaggtcttg ccaaactctg caagtgttgc aggtgttttt
    37021 tcacctagtg agcgaattcg ctctgctatc tcatcaatgt cgccatctag ggctgcgtat
    37081 tgatctgcaa agagcttgtg gtactcgcta aagtgcggtc ctaccacatt ccagtgatag
    37141 tttcgaatct ttgtgtacaa gacatactcg tctgcaagta ttttgccaag gatgcgattg
    37201 acttcttgtc ggtctttgtc tgagattcca atatcgattt gcttcatgcc acttattagg
    37261 aactattcct atttaattgt tattattagt taggaattat tattaataag gattaaatac
    37321 cgatcagaac aagtaagaac atcaaacatg aagcaacacc tcgaagagct tgtaacatcg
    37381 ctaagaaaag aaggatttag aataactccg cagagaatag caatcgttga ttacgtgatg
    37441 aacaccgaag aacacccaag tgcagaacac atccacaagg tgattcaaag gcaatatcca
    37501 atggtgagcc tctctacggt atacaagaca ctagaactac taagagaaaa aagacttcta
    37561 aatgagatcg aagtcaacgg cgaatcaagg tttgatccaa acactgggga gcacgtcaat
    37621 ctggtatgcc tcaactgcgg catagtggaa gactctgatg aaggcgggct aagagaaatc
    37681 cagaacaaaa ttgccagaaa gtcaaaatat ctcataatac gaggcagcct tgatctgtac
    37741 ggttactgca gcaaatgcaa atcaaaattt gaaagatagg tgacgcactg catttcactg
    37801 ttttgaaacg gctatatgta catatacgta ttatagtact acatatatac atgtacgtac
    37861 atattaaaac cagatgagta aagttttaca aaataaaaaa tccacacctt ctcaagcaaa
    37921 gactaagact agcacagcta atccaactct agatacaatc aagatggtag aacaagcact
    37981 ttctaaaatg tcagaataca catcaaaaaa caggctctgg agggcactgc caagacaagt
    38041 acaatatcca acatttaagg cgatcctgga ctatcttgag gaatcaaata agataatgta
    38101 tgacaaggat ggtacaatta tctggatatt tgcggacaat ccaaaactaa agaagctaat
    38161 gcgaacctca aaatcattat tgtagtctag taaccaaatc ttttctcata ttctttatgt
    38221 gaaaatgctt caataataca aacaacaaaa ccgtcttttt ctcccattat tgtgtacact
    38281 agtctccaac ctgaacgcaa tgggagtctc caaagatttt ttattttgta ttttttgata
    38341 tatgactgag gccataggtc gtgagtaatt ttattcccac acaaacagtc ctcctgtaaa
    38401 gttttgaatg cattatgcaa ttctcttttt tgtggctcat tttcagaaat ttgattaaag
    38461 agttttctaa actccttagt gccaattaca tcctttgctc tttttgacac ataacatcta
    38521 acatatatca aaaagataag attatctgag tgtaaagatg atcagtttgg ttaagcattg
    38581 atgtaaatta tgagatttga caaagctatt ttcttagctt gctttttaca attctgcaga
    38641 tttcatcagt catttgtgtg gtagttgaag ttccgccaat atcatatgtg acgtattttc
    38701 cctcattaat cacctgctct gttgcatcaa aaattgcatt tcttatttga ggttctccaa
    38761 gatattcaac catccatgcc cccgatagta tggccgctgt gggatttacc ttgttcattc
    38821 ctttgtactt gggagcgctt ccatgtgctg gctcaaacat ggcatatgaa tctccgatgt
    38881 ttgcagaata cacatttcct atagagccaa tgttcccaga cgcacattcc gatatgatat
    38941 ccataaacag atttgtgcta agaagtacag aattgttgaa tctttctgga tttttgacca
    39001 gttgttgggt catgttgtca atataatatt cctcaacatc aattccggag ttttctttaa
    39061 ttgccttttc aacctcgctc caaaatatgc catcggtttc tttgagtatg tttctttttg
    39121 tgatggcaaa gatttttttt agattgtgct gctttgccca caaaaaggcc gcctttacta
    39181 ttctctggca gcccttacgt gtggtttttc ttattgctat ggccgcatcc tcagatattc
    39241 taaactcgat tcctgcatag agtccttctg ttgcctccct aaagcagaca aagtcgagtt
    39301 ttttgtctgg agagagtcta tcatatgtct tgattggtct gatgttgctg taaagctcaa
    39361 acttttggcg aatggtgact gctacacttc ttggcgcatt tggttttggt atggtagtgg
    39421 ttggaccttt gtagcaagca ttgctttctt caagtaactt cattgttgaa tcggggatat
    39481 atgtagcgcc tccatgtttt tcccattgtt cagagccggc atcacacaaa attagctcag
    39541 cttgtgtgtt acatgatttc aaaacatgta gcatggcatc aacaagctcg ggaccggtgc
    39601 cgtctccttt tatgattgca gcttgctttg ccaaaacagc gatgcttgta gatccagtat
    39661 atttaactct aactagaaaa ttttcacttt tttctgaatg tatgatccca acgcataaat
    39721 ttctggaaaa ttatgcgcca agatcgtaca atttcagaaa actaaattac tacatttctg
    39781 ttaaaacgtc tgatgataat agtccaacta tccgacattc atgtgggctc ccaattccgg
    39841 gaggatgttt ttgaaaaggt aatcagggag gtaaatgcgc tcaagcctga cgcaatcata
    39901 gtgacaggcg accttacaaa tgagggccta ccaaaagaat acgaaaaatg caaaaaacta
    39961 ctctcaagac ttgagacaaa aaaaatcatt acaatatcag gaaatcatga ttataggaac
    40021 acggggtatt tgttgttcaa aaaacacttt ccatttgaga cggtaaacga gcttggcagc
    40081 gatattgtgg tagtgacgct tggtactgca aggccggaca gggatgaggg cgaagtagga
    40141 taccgacaaa atttgtggct tgagagaacc atgcaaaaat acaaagacaa gataaaaatt
    40201 ttggcaatgc accaccacct gattggaatt ccggacacgg gatcagcaag agttgaggta
    40261 attgatgcag gcgatgtgtt gcggacaata cttgcaacaa aggttaacat ggttttgtgt
    40321 gggcacaagc accgcccgtg gatttggaac tttaaggagc tgtcgattgt taatgcagga
    40381 actgctgctt ctgaacgggt aaggggcttt tttgagaaca catacaacat catcaccatt
    40441 aagaacaaga aaatacacgt tgatctgaaa atcgtgggcg gaaaaaagat tccactggaa
    40501 agcatcgttg agcattattc aagatctgaa gagtgaattt atttacagaa ccaccttagc
    40561 aacccttgag cgggggtcgc ctagcctggt agggcgtggg attgctaatc ccatgtccgc
    40621 aaggacacgt gggttcaaat cccatccccc gcgctttatg cttaaattta atagggtatg
    40681 ccagacccat attggaatat gggacgcaga aaaactcaga agataattag aaccgcccca
    40741 aaaaacgtca caacgggcct ttgtcccaaa tgtcagacgg ttggtcgaat cttccttttg
    40801 ggccaggttg gtgccgagca tgcaaaatgc gctgcgtgca accaagagtt tgacctgtag
    40861 caagtcaggc acaaaaattt cacccataaa tttttaagct cttttttagt ttgtacatct
    40921 ggacatgagt acaaccgaag gcgagacaat ttatgagcga gtagccaaac tcgaagacga
    40981 agttgcagta ctgcgcaacg aagttgacat tttaaaaaag gcactacgca acaaactggc
    41041 tcgacatgaa atctcacaaa ttagaaaagg aaaagacatt agctcaatta tcgattaatc
    41101 ttttctcttg tgtttcgtat aagctccaag acaaaatcaa cgtcgggggc ctctggggca
    41161 atctccagat atttgtttag gtattgtagt gcgtcctcgt agtttagcag cctttcctgg
    41221 attattccct tgtcgcgtat ctcatcagga gagtctggct ctactgctag gatcatgttt
    41281 gcacacctca gcgccttttc gtacgcatat gactgagcat acgagttttt caggttccgt
    41341 attattctga tgagcacctt gtcttctggc agctcgtcta gaaattccgg cgaaaactcg
    41401 atctcctccc caaagttcct gtacaggatt tcctcaagat cctcgacgct gagtggcctt
    41461 ccacccccaa aggggtctaa taccatctcc tcgccgtact ttacaactac gtggcttgga
    41521 aagccgcaga ttttcagatc aaggccgatt cgttttgcga cctcgacata aattatcgac
    41581 aagataatgg gtatgccgct ttttttgtcc agtacctcgt ttagaaaatt attttttggg
    41641 ttgtaatagt cctctgtgtc gccactaaag ccaagtctgg tgaataggtg ttcattgagc
    41701 atcgaaatca gatatgtggg gtttttgaca tcagagagca ttacttggag cgactttgca
    41761 atctggttta tcttttcaac ttgatcctcg atgtttaggt ccggatactc taagatctgt
    41821 ccaagcttga ggcatttttc caccaagttg tacttggaat cagaagaaaa ggaaacccac
    41881 tcggcgacaa aggggtcaaa tttatcattc attgatcttc tcagattctc tttagatata
    41941 actcggtctg ctttagctcg cgtgtagtgg gtgggttttg aatcaaaact gaaaatgctg
    42001 acttgcctag cttggaaaat gctgcctcaa ctaggtcctt tcccatgtgc tttcgtacct
    42061 ggtacgatga gacgtcggtt cccatgaatg tggtgaggta ctgcgcaaaa tcatcgtcat
    42121 cttttagtat gttttgtacg caaaattcgg ccattccctc cattgtggaa aacgctgcat
    42181 ggtgcctttg aattggctct agccaacgct ggtaaatatc gagaagctgg ggtacaagct
    42241 gggatatgcc ggggtcgact tcgactttgg taaacgtcat gacgtctggg cctagaacat
    42301 ttacaataaa gttgtccgga tttagcgtaa agaacaggtt tgcgcgcttt gctaatggaa
    42361 agtcgtctgg cactgcaggc agctgcaggt aatctgcaag ctttgtcgca gtggaatcat
    42421 caagattcaa aattattttt gcaagctctt cgtttatctt gtttacctcg attactgcct
    42481 ttttgttttg ctcgtagagg ttttgctgaa tggagtgaac caattcttct agcacagtca
    42541 tctttagtgc gccgatatat ccagatttta cagtctcgta ttgcagctcg tatggcttgc
    42601 cttgcttgtg caagatgatt tgcggatagt ttgacaaaac gaaattgttg ataaatattg
    42661 taaagtccag ataatcgccg tatgtagttg agatctttga gatgtaggcc ttggcatatg
    42721 tggagtagac taggaacttg gtagtgtcct gttttatcag ctcgacaatt gcgccactat
    42781 cttgttttgc aagtgctgca aacagtctgg ctacaaactc ctgcgagtcc tgagtggaaa
    42841 agacctttct tcccttgatg ttttttagct cctcaagatc ggggtactgt attttgagat
    42901 caggcacaga aatgccggta aattccgcta cactctgctt tagtcctggg aggactagct
    42961 ctgttatctg ccttggcgag tcaaatctta ccttaaagtc tgggtcctct gcgattttcg
    43021 aggcaaggct tgcgatgtct ttttcctcgt ttgtctcaac tgagatctgg tcaagcaggg
    43081 catctgcaaa ctcctcaagc tcgctcatca cggtttgtca ttttttgtca caatttaacg
    43141 gtaactagca catagtatta atccgattta gaaactgatc aaaactattg cacaaattca
    43201 gcttgggtta tctgaggtgt gttaggtgtc actccacgct ggatgtggaa attctaaggg
    43261 agggcacaca aatagaagaa ggctttcttt tatgcccaaa atgcaagatg tattttccaa
    43321 taattaatgc gattccaatc ctttggaatg attttgccaa ttatttatca aataggccaa
    43381 ggcttggagg cgagtttcta actagtgcaa atacttccaa gatgcgatcc tttgtcaagc
    43441 aaacactatc caaaatccgc aaaaatccgt ccgaccaatc catagttgaa aagcgctggt
    43501 ctgaaatcta caagcaaaac aaaaactcga cattttactg caaaatcaag gagcatctaa
    43561 gaccaattgc aaactctggt gtggtgttgg aacacggctg ctcaattggt attatgacgc
    43621 aacatctggc aaaaactaat cggctcgctt ttggaattga caaatcatac tatgctatct
    43681 ctgaagcaaa aaaatccaag ctaaaaaacc ttgatttctt tgtggcagat tctctggaac
    43741 atccatttgg aaaaacaaaa tttgatttgg tattggggct gaacctcttt gagctaatcg
    43801 aaccaaaacc gttgctaaaa ttgcttgcag gccaagtgag aaaaaaagaa tggctggtac
    43861 tatcagaccc atatgatttt gacaggggtg caaaatctgt caaagaacct ctctatgagg
    43921 actcgatacg aaaggaaatc acaagacagg ggtttgccat atccaagaaa acgacaaagc
    43981 caagtcatct tttgtgggat ttacggcttt acagcagagc aaacttgcag tataaggtag
    44041 atttgatcat aggcaaaaag actagataga tatcaaagca agcttgtttg ctttaaccat
    44101 acaacatcat ttgctcattt ttaccacatg atgaaattgt atgggtttta gccacgtcgt
    44161 tactgcaggc attgtcataa tacccctaat gctaattgcg atgaatatac caaacatatc
    44221 aaatacgatt ttggaaataa acgaagccgc gctagatgtg agcgagctag aagatgcaat
    44281 actgaacact cactttcaaa ttttaaacct tcaaacaacc ggtctcaaca ataggatctt
    44341 ctttgatcta accaacactg gaaccaccaa actctgggat tacgaaaatt ttgatgtaat
    44401 actcacttac gaggggattg taaattctgt tgccacaaac gtaaccgaag tccttagcta
    44461 ttctgagctc gcaccatctg atgacccaat actgtttgat gctgtaacca gtcatactga
    44521 cagctgtctt gtgatactta atccttgtac attttctcac actgtgacca attctggaag
    44581 cgacagaatt ctaattcttg cgataagcaa gcaaaacaat gcagtgctaa gtacagtaac
    44641 atatgacggt gaagatctga cagaacttcg agccgatgac gttgatggaa atactaatgc
    44701 gcatacttcg ttgtggtatc tagtagaccc gccaaccggt tcaaacactg tttttattgg
    44761 tgtaggactc tttgacacag atgttgtagt tggtgcaata tcttttacag gagttaatca
    44821 gactgatccg tttgacgcag taaatggtgc aaccggaaac tcggcaactg cggcgacaac
    44881 tctgactact acaacaaatg aggcatggct agttgatgta gtggggacca acggtggaac
    44941 agtcttagtc ggtgcaggcc agcttgaacg ctggaatgat gttcaagcct cagttagggg
    45001 ggcaggcagt accgagttta caagctcagc tggcacattt tcaatgagtt ggacaaaaag
    45061 cgcaagctca aactgggcaa ttactgcagc tgcattaaag ccagctaatg ctgcatgttg
    45121 tgtgtctgta gggaattggg ccataaacaa catttcagac gattacattg atccgggaat
    45181 aatcaacaaa gatgaaactg cggcaatcag ggcaaagaca tcatatcaaa tcataactaa
    45241 cggggatatg tacgtaacag ttgctgcaga aaacggggta agagcctcat catttgtaaa
    45301 cgcacagtga gtttatgatc ttatttactg cagaaattga ttttccattg agattatcaa
    45361 tttcaataat cacgcttacc attaagtatc acaagaaata acgattcaca atggcaaatt
    45421 tcaaagcgct aaaggaatcc ctggacaaaa acggcgagtt aatgataaga ctagatactg
    45481 gcgagaaaat cgagctacac aaacacaatg tcagcttcaa cgactctaca caagaagtca
    45541 tagtcgatgc ggcaactgag gcttactgga ttgatgccgg caaaatcgcc tattactgga
    45601 ttcaccgaga aggaatagaa ggaaaagagt aaacccaccc tacaattttt ttgttctcaa
    45661 aaagatacaa agtttgaatc gatgagagtc agaacaacat aaatggattt gtgtcctaag
    45721 actaaatacc ttgaatgggt acaaaaacat cttcaaggtt ttgtacccat ggcgtccaga
    45781 tcggtttcat gtgtgaatgc ccagccatgt aaaagtcagc aaaataacaa ccggtatctt
    45841 ccagaccatg tgtgtcaaca cctcgtctat ctaacaattc tatggttttt ttcatttcct
    45901 cctcattgta aaactgtctt tgttttaggg gtccaatctt tgtcttgatt aactcaaagt
    45961 tatttttttt aaagactctc tctatttcat ccatgtgaaa cattcgaaga acagtgaagg
    46021 caaaaattgt agatgagttt tgatcatcga tattctcaaa catcttttca aaagatttcc
    46081 atccaatata cccaatacag ccagtagaaa taatcaaatc gatattgctc aaagttagtt
    46141 tgaattctga ggaaagtggc tctttttcca tatttacaca aaaactccct tcacacagac
    46201 cagctttttc agcaaattcc aaagcgggtg aggaagtatc aactagataa aatttaaggt
    46261 tagggttttt gccaggtaat tcatcaaaaa agtcttgaac ttccttgatg gagggtctgg
    46321 gattctgttg aataaaaaaa tcattcaatt ctgacattat caactcataa tttaacaatg
    46381 cagagttgat tccataggag cttcccaaat caagaatgtt aattgatctt ttaagatggt
    46441 ttcccatccg ctcagtaagg tgtttgtaaa gcggtcttgt ttgatcaggt attcggtatt
    46501 caacatgatt catttcttct aggtacggtg ctgggtattt tttcgtataa atgtctgaaa
    46561 aatctttctt aactactaat tcctccatag tggataatga tgaagcctca tcccttataa
    46621 aagaacctca aaaaaatttt cttaaactaa aaacatgatt tgaatttttg attatttttc
    46681 gtattcaaga attatgtgat ttaagactct aaatttatct cacagatgat tttttctttg
    46741 cggcctttct tcttacagta tattttcttc ttttttgcgt gattctgctt attgtctttc
    46801 cacgaagttg tgcctttcgc ctctgcccaa ttctgatttt ttcatgtttt ctgtcttctt
    46861 ccatcttttt gagaagccgt gaccgcgttt ctacgtcttt gtttgaaaat ctcaatattt
    46921 gccatgatta tgatgctaat tttaagtttt taatacgcat taaatgatgt gtgctttctt
    46981 ctaggtctgc attaaaggaa acacctaatc gataaccata atacaaacta tgactttact
    47041 aaatgaatat tgaaaagcgg actgtcagtt attctagtta aagacatcat gtcaagagca
    47101 ctaataactg tggatgcttc aacaacgatt tatcaagtag caaagatgat ggagcagggg
    47161 aggctgggcg ccataatagt aaagaatgat agccaccaag gaataatcac tgacagggat
    47221 tttgcaataa aaatagctgt aaacaaacat cctttggaca ccccagttca taaggtagcg
    47281 tcataccctt tacagacaat taattcaagc gagtcgattt tggccgcagc ggattccatg
    47341 tctgcaaaaa aaattcgaaa acttgtggta gttgaagacg gcaaagtggt cgggatcatc
    47401 acatcaacag atctggtaaa ccagttagct aaaatgaaaa atgattagaa tctaggcaca
    47461 agactggaca agaatagatt attgtagaaa atgactgtgt tctgtttgaa tattaaacag
    47521 aattatgttc gctgttttgg gctaatttta ggcccatgag aagcaactgg tcaaaagtta
    47581 ttagcattca caatgatgtt aaaagagatt gaaggtttac ttggatactt ctgtcataat
    47641 cgcgcttgct gaccctttgg atgtgtttca cgaacagtcg atgagttttg ttaacaagct
    47701 ggtcatgtac acgattgaat gcatgtctgg cagtccgctt ataatggagc tgggaaaatt
    47761 agttgaagcc aagggaacaa aaagatgtct agatatcatc aactcgatgg aagaatttgg
    47821 aattcatctc aaaaacatag acatgaaaca ggcctggaaa ctatccgaga tttatcttga
    47881 tgaaggtgtg ctaacaaaaa gacattgact tgatttgcta cattatgcat ctgcatcatt
    47941 acttggctgc acacatctgg catcatggaa cagcagacaa ttcaacgaca ggatatccaa
    48001 gaaggtaagc aatgcaaatg cgaaacaaag actgtcaaac ttaatagcag gcaaaccaga
    48061 gtatattatg aggcaagaaa atcttgacta aacacgacaa cctcaagatg ctggaccaaa
    48121 tacgcaaaga gctatcacaa gatctggaaa agatgacgtc acaacaacgc gaggaatttt
    48181 tcaaagggac agaggagatt tatgaaggtc taaagaagat ggcaaaggtt aggcaatcta
    48241 ttactagata atcacagaaa atttacgcat gtcagagttt ttaacacaat tatgtatgcc
    48301 gggggtgggt tttgaaccca cgacctccag attatgagta ttgccccttt tattgggagg
    48361 atagaaagac tggcactcta accaggctga gctaccccgg cacaagaacg gctaatttta
    48421 tggcgcaatt aaatgattct gaaatttgtt atgtaatttg aaaaagtaca atcagttatt
    48481 gcgcaactat gtcattccac ttggcatctg cgtttttgac ccagaggaat tttcgctgga
    48541 gtgatgttgt gtagagtttt tcccagtctg cctttacttc ggctgtccac gccttgaaga
    48601 cccggggatc gatatagttt cgaagcgacg ttcccagatt gtagtcacgg gtttgtcttt
    48661 gcaactctat tgccagcttt aacttttccc ttctttcttt gagcttttct ttttgcttgt
    48721 cggtttttgg ctctgtatgc tctagttttt tgagctgttc ttttttcttt tctagtgtgg
    48781 catcaaagtt ctttgggatg gttcgcttgt ggttgcacat catggctgcc tggaggtttg
    48841 ccattttcgc gatgtagagt ttttcgtttt ccgacttgcc tttaacattg gtgttgtttt
    48901 tgagatagtt tttgacctca gttgttgcca gatacgttct gaataccttg gcagtcatgc
    48961 ctttgactac ttcgcccaga aatgcattga cgtgccttga tgttatgcca tcaaagatct
    49021 cgtcctgcgg ctttttgttt gagatgagct ttttgatgtt ttcataaagc ataacgtcgt
    49081 ttccctgaca tttgatggtc tcctcccatc ggacggaatc ttttcctaaa aagtcaaagt
    49141 ggatgctgtc gttgccaaga ttgatgtgtt cttttcggag tgttgtggcg cctaccgtgt
    49201 ccgcctcgtc tgtgtctttt tcgtctccga ctctcatcga cgttctataa atcagatagc
    49261 acgcagttgc aatttttgat acccttgggt tcctgtcttt cattgccgaa attatttttt
    49321 cttcgacctt tttgatctct tttccaagct ttgccgcctt gtcgtacttt gcctggtcgc
    49381 gctcctgctt taggcctgca gtgtccgcaa gccagacgta cttgatcttg ccagagataa
    49441 agtcatccca gcatgcaagc cacatggaat cgtgctcatg gacaatcttc ttccagtttc
    49501 ctggcggaac cttggcctcc ttgcccaagt tcagcgtgac gtccttatgc gttatttttg
    49561 gcttccactt gccacgcaat gggtggtcac ctcggccaat gaatattcca ggcggctctg
    49621 ccatgtagtt tgctacttcg acttcatgcc catcaagtat tgccttaccg taaactgact
    49681 tcattttttc ccgcagctct tttcgttttt gggcaaggga ttttttctcc tcctttgtta
    49741 ccagaagcct tgcgtccttt tccttatcga caaacttgaa tgcctcagaa aaatcaatct
    49801 cagaaatgct aatgcctttg aacttgcccc caaaggtggc agcaaaatct gcaacaaagt
    49861 ttttttggaa aaccgtatcc tgcacgtagg gcgtgtcctt tttctttgcc cactggtata
    49921 tcatctcttc ctgtagtgtg tctagtttga ctggctggcc ctttattttg acagagatgc
    49981 gctttgactc aaaatcgggc ggaaagatta tgccgttgtg ttcaagggtt ttccatttca
    50041 tgattcatca tttgtggttt aaagtgagga ctttgataaa aactgggttt tttggctata
    50101 tcaatttaac ccatcaaaaa agctcttttt ttagataatt ttcaaactcg atgtcagtct
    50161 tcatcttttt tgcctccaag aacatcactg cgtcatttcc aactgggtat cggggcaacg
    50221 ggtctttttc ccttaacgcc ttgactatga catcggcaac ctcttttggc ggcgtgccca
    50281 tttctgccat catctttatg ccatttacga ccttgtctgt gaggtccttg tatggggagt
    50341 caggcttggc attctttggc attctcaagg ctccaaaaaa gttggtctta attgcgccag
    50401 gctctatgat tatggtgttt attccaaacg gcgacagctc gtagcgcatc gactcgctta
    50461 atccttccag agcaaacttg gagctgatgt atgcaggcgt tccaggaaac gcgattcttc
    50521 ctgcaactga gctgacattt acaatcgttc ctgccttttg gcctctcatg attggcgcaa
    50581 cctcctgaat aattctgatt attgaaaaaa agttcgtctc aaactgttct tttatctcat
    50641 cgatgctcag gtcttcaagg catccaaaca atccataccc tgcgttgttt accagaatgt
    50701 cgattctttg tttttctgcc atgatctggg agatggctga ctttactgat tctggcttgt
    50761 caacatctag ggcaattacg gaaactggca ggttttcttt ttttgcctca tctaaaagtg
    50821 gttttgattt ttttgtattc ctcatcgacg cgtaggtata gtatccctcc ctggcaaggg
    50881 caagtgctgt ttcaaatccg attccacttg agctcccagt aacgagggcc actttttgca
    50941 tggtttttgt aaattactca ctcttaatta attatcttga cgttgcaaaa tcatatacta
    51001 gacaaggggc ctgtctcctt cggtggggtc gattatctcg gtgacctcgc catcatttat
    51061 tctctgcagg atttcaagtg ctgatccttt gtgcaaaaac tcgttgttgt tgccgcaccg
    51121 atatgaaaag gtgcaccgcg gacagcctgc ctcgtttttg caggggcact cccttacaat
    51181 tgagaggctt cgctcaaatg ccttttccaa tctgtcgtat agtgccttac ttgccccgtt
    51241 tccgccgatt gcgccatcat aaatgaagat gagtcctgat gtgccaagag atatgccgcc
    51301 taggtcctgt gacacaccgc cagtcaccat gttgcttccc tcaattatga tgtgctctgt
    51361 tgcgtgatat ccgcttgcct ctacatattc cggattttct gctagctgga tttggttttg
    51421 tggcactggt gcatgaatga caatcccctt tgtgataaaa tcatactcta gtggctgctc
    51481 tagcagaatt ttctgtccct ggtttacctc ctggcccagc tcgatattga catatccgta
    51541 gacccgtttt tgtatgtgga gcttgcaaaa acagacctct attccaactg cctgcctttt
    51601 ttcgtatacg gtcttaattg ttggccactc ctctgttagc gccttggtgt agtagggata
    51661 gttggatgga agaggtgcta gcttggcgta ttgtttttgt gggtatccaa actcctttac
    51721 cttgtagcgg gttccagcca aaaaatagac tgcggactca tgcagctctt cgagggccat
    51781 ggggaggacc ctgtcgccta tttttttgtc gttgtaaaaa atgtcaattg acttgccaat
    51841 tcccctaatg gaataatcag aaaaaatgga ttttaccgaa tcaaaattgg ggacgtacct
    51901 gtcctctttg agaactaggt ttccgcactc gacgtgtttt cgtattacac ccacatgctc
    51961 gctcagctcg tgcctggcaa tgggcttgtc tgctgccatt gcaaggacgt gaaactcttc
    52021 aacaaagggg ttctttgggt cgatgtacgt tttttcaacg tcctcaaaat aatcgtcagg
    52081 atggttcttg tagtattgtg atatggggtc gttgccaagt gccaaaaacg caaatccctt
    52141 ttgtcctttt cttgcggctc gtccgattct ttgcaagagc ctgttaattg gaatcgttga
    52201 tgaaatcaca ccatcaacat ttccaacatc gattccaagc tcaagtgtcg gtgtgcagga
    52261 aattgccatc agcctgtctt ctttgaatga tttttcaaca aagctccgat agttggccat
    52321 cagtccggcc cgatggaccc gaatgtcaat cttctttttt ttggcctgca ttgccacgag
    52381 ctcagaattt aggtgcgagt tggaaaacac catggtcttg tgcttttttg cggtgagctt
    52441 ttcgagcagc tcaactgtca gggccctctg ggttcgaagc gacggaaata tcattacaaa
    52501 gtccgtctcg ccctttttgc cagagcccac aattagctgc atttttacac caaagagctg
    52561 ctcgcaaaac tcttttgcgt tctctagtgt tgcagatgat gcaacaaact ggatcttttt
    52621 gcagattcgc tttaaccttt taatgatgta gtgaacgttt gagccaaaaa ttccggaata
    52681 aacgtgggcc tcatccacta cgaggaattt tgcagtgcta aatgcagatg caaaccgcgt
    52741 cctgtacatc atatgatagt gcagcacatc aaagtttgtg acaattatct ggggcggctc
    52801 atcgaggatt tgggcccgct ctttttgttt tgtgtcgcca tcaaagactt cgactcggac
    52861 tccgactctt tgcgcaacct ctctgagctt tggcagctgg tctcgcgaaa gcgcctttgt
    52921 ggggtagaca aaaattgcta aaatcgcgcc tgactgcacc tcgtttgcaa tcttttggat
    52981 gattgggatt gcaaaggcct ctgtttttcc agaggcagtg ggcgcagtaa ttaccacgtt
    53041 ttgcccaaaa atgatttcat tgattgcctc ttcttggaac ttgtagaatt gctctatctt
    53101 tttttgctgc aagtgctgga ttattccgtc atcgaggccg cactcatcta ctggtgcacc
    53161 aagggctggc tctggctctt tgattgcttt gtaaaacgag acataatcct tttttgagaa
    53221 aagaacgtct cttgttattt cgtctggact ggagtctgcg atcatctttt caatttcttt
    53281 tttgtcgcga attattccgg cagtctttag ctccagtcca agctgttttt tggtgggtgt
    53341 ctccccctgg tcgtatcgag ctaaaaactc caagtacacc tcgtcgatgt tctttgtata
    53401 gtcaactagg tcctggaggt cgcatttttg gcatgcgatc atgtattttt tgttaaatgt
    53461 cttttggatc tgcagctccg acttgcattt tgggcaggaa tacctcaact atatcacacc
    53521 tatttgggtg cagtatggat ttattgtttg gttggtgtac ggcactgatc tgggggttgt
    53581 gccatgctat actgggcaat catccgaccc tccaagtgtt tacctgcatg gatctagtca
    53641 accttaactc atcaatcatg ataatatgat aaacacgatc cagttatcta tgaggatccc
    53701 atctacaaaa ccatgggaaa tagttctgga agctacgtca gaggcgtagc tgcaattcac
    53761 aaaaaatacc aaacagccct aaaacacgca aggagcagac aatcagttct taatgcttat
    53821 tggaggcaca aaaaagagtc tgaacggctg ctggcaaagc atctcagaga cgagatggcc
    53881 gaagtaaagc gcataaaggg caaaatggaa tacagatagt caaatatggg attgggacgc
    53941 aagcccaaat tccgtccttt ttgtgttttt acgccttgac gcgatacatg tttttgtcaa
    54001 gcctcttttt tgcaaagtcg tggtattttt tgacaatctc aaagccaaga tagttccgcc
    54061 ccatctgctt tgatatcatc gccacctggc ctgatcctaa gaacggatca aataccacat
    54121 cgccctgcct actggaatat tctagaattt ttctgagaat ctctgccgga agctttgtcg
    54181 gcgttttctt atcaccagtc cagtactcgc gctttatgtc ccagacgtct tctttgtctt
    54241 tgtagtggag gctgcggccc tcagatgttt tttgtttttg tgaaaacctt gtgaatgggt
    54301 agaacttgcg ctttttttcg tttttgcaca catacacgca atgatagtga gacgtgacaa
    54361 actttcttgt ggttactacg ccaaactggt acttccaaat tatgtggttt acagtcacaa
    54421 acccgacgtc gtctagcgca ttgaggatgt ccttgagatt gttccaccca gaaaaaacgt
    54481 acatacttcc agactctttt agaattctaa atgcctgctc catccatttt actgaaaaat
    54541 cataatagtc tttttgttta atctcgttgt atccctgcat gacccgcgat gcggttctgt
    54601 tgtagttttg ttttgtagcc ctaaagtcaa ttgcaaacgg gggatcggtg acaattagat
    54661 caattgtctt tggtagtatg tgctgcatgc catcaataca gttcatgttg tatatctgat
    54721 tgaatgtgat ttttttcaaa actagcttct gtccttttgg gttgtatcgg attgagtgga
    54781 cattttctcc gaaatgatct ttttgagctc atcgtttgtc tttcctgtaa actccacgcc
    54841 aagagatttt gcgataaact ccagcttttg gcgctcgttt tgtacgggag caatcacatc
    54901 aagatttgta tttacaacgc ccgaaatctg gctctgaatc tctttttttg tgcggttgta
    54961 ttcgcctact acgcggccga gcttttttgt cacctctgga agcctgtttg tcccaagaag
    55021 ggccaaaagt gccacaaata ttatgataat ccactcgcct ccaagtatgt tcagcgaata
    55081 ttccaacatg tcttgatttt gcaaggtgcg aaattaaagc ctagtggatt agtcgaaatg
    55141 tactgataat ttgatagaag aattgctaca caagctaatt aaacgtctaa agaccaatta
    55201 gtacgctagg taaagattga agggactaat tgccatttcg gctattctga tagctactat
    55261 ggccatcgca ccggcatacg cagtaatcgt agagtttcaa actgacaaga cgtcatacaa
    55321 aaaaggtgat gtcataaaat tcagcggaaa gacggacatt gcacattcaa acaagatggt
    55381 ctcagtaaaa gtctatggcc ctgatggtga atttgtgatg cttactggcg gaagatccga
    55441 tcaggatgca attctcgtac taaatccaat tgatacatca aaggaaaaga ctgtggccaa
    55501 attttcacaa aaaggaatct ataacgtgac tgcattttat gatctagagc cgacatatgc
    55561 tggcaagtcg actctttttg attactctcc agatggctca ccggttagtc cttcggcagc
    55621 agaggtaatg ggccagcaag ctcaacaacc gacacaaact cctacacaac aaccgacaca
    55681 aaccccgact caaaccccta cacaacaacc gacacaaacc ccgactcaaa cccctacaca
    55741 gcagccaaca caaactcaac aagaaacaca agaacccaag tgcgggccgg gaacagttct
    55801 aaacaaagac ggagtctgcg ttattgcaga gccagagcct gagccagaag ccgagccaga
    55861 accggcacca gaacctgaaa catcaggacc aatggtgact cacatcccag gattcccgga
    55921 cccggacaag gatccacagt attacatcga taggtacaac aacgagcctg cctacaagga
    55981 atggtttgac agaaactttc cagattatac aatttatcag gtattagggg tgctaggccc
    56041 aaagaagact cacatcccag gattcccaga ccaagacaag gatccgcaat attacattaa
    56101 caggtacaac tctgatgcag aatacaaagc ctggtttgat agaaactttc cagacgatac
    56161 catctatgcg atagtggggg tggaggagcc tgactcagag cagactgatg ggtgcggagc
    56221 gggaacacat cttgaagatg gggtgtgtat cttagatgg
//
LOCUS       DASWKH010000005         6296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_750233, whole genome shotgun sequence.
ACCESSION   DASWKH010000005 DASWKH010000000
VERSION     DASWKH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750233"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGA92_00580"
     CDS             <1..245
                     /locus_tag="VGA92_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEX9844939.1"
                     /translation="KTIGKFDLNVQDAANNLSPKVIAKYCYNLAVMFNAFYEHVRVLD
                     IKDVSLLNARLCLVHSFKSCLEEALGLIGIETPTRM"
     gene            461..841
                     /locus_tag="VGA92_00585"
     CDS             461..841
                     /locus_tag="VGA92_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9844940.1"
                     /translation="MVKVIVVDDDVDTVEVFCEYLEIKDITVLDRGYNGKTAVELYER
                     FKPDIALLDVMMPEYDGFYGLENIKKINPNAKVIMVTADLTYDTEKQLKALNASAVIY
                     KPYEIDSVIDTIHKVHKGAVLLQQ"
     gene            883..1380
                     /locus_tag="VGA92_00590"
     CDS             883..1380
                     /locus_tag="VGA92_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9844941.1"
                     /translation="MKILIADDEPDLLSQFRAVLVDRGHTVVTTGDGDACVMAYSKEY
                     AANPESPFDVVVLDYSMPKMNGLDAAKEILGIAPSQRIIFASAYVQETLVDSIKNLKQ
                     IVELLQKPFSLQDLIDTIEDKTIYEDLAKLNVNIQNLRDLNPTHAQIRDYLDALRKLQ
                     KAKTF"
     gene            1420..2226
                     /locus_tag="VGA92_00595"
     CDS             1420..2226
                     /locus_tag="VGA92_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX9844942.1"
                     /translation="MTKIKNNSYVLFFYNDSKKWLNKISKDQALHTHVGVIKHSDALG
                     KEYGSRVRTNKDKYVYLLEPTIHDFVMKSQHGTQIVYPKDLGYIAARAGVQSGQTVVE
                     IGTGSGALTTFLASIVKPRGHVYTFDVEPKFMEIAKKNITRAGMIKYVTMENLDIKTA
                     KKMPLTEADIAIIDLGDPWSVVPQVRKMLKGSGAVIAICPTMNQLEKLTGSLVENEFT
                     DIECSEHILRTIDAREGKTRHSFYGIGHTTYIAFARKAFFDRKIRKDNDA"
     gene            2299..2823
                     /locus_tag="VGA92_00600"
     CDS             2299..2823
                     /locus_tag="VGA92_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844943.1"
                     /translation="MLSESKKPEKYVASQINKARKSLETFGTVSPENHITKEDLQKLG
                     LADEIRKLKSIQREKLLEDLSTLLNDKIREFEKHEQEMKWKDSMLQRLNTELSEKITQ
                     LEKTNKQLVEEKARSDELNRQLQETLLKLHSSEEQLKLERDWLAEQVETKSKEVLETI
                     REMIKDAETQNKTA"
     gene            2869..3732
                     /locus_tag="VGA92_00605"
     CDS             2869..3732
                     /locus_tag="VGA92_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9844944.1"
                     /translation="MAQKLQSALIKKFIPARKPDSRKGQNGKVLVVGGSYMYHGAPVL
                     SSLSALRSGVDLVYTAVPKIIAPSVRAISANLIVIPVVDSKLTRGATNKLLGQLPTDL
                     DSATIGMGLAIADEEALKNLVRSLISQDVRLSLDASALVSYILPFISEKNIVVTPHAG
                     EFKRMFGIMPSASIKERVKTVEKLAKEHSVTILLKASTDVISNGKQTFVNPKNLAAMT
                     VGGTGDVLSGLVAGLLAKNRNPLESAAAASYINGLAGKLVQRKNGLHMVATDLIDTIP
                     TVMKPFDKVTR"
     gene            3729..5084
                     /locus_tag="VGA92_00610"
     CDS             3729..5084
                     /locus_tag="VGA92_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9844945.1"
                     /translation="MKKILTHVDKNFGSLISDLQVLIRQPSVSAKNQGIEECALLVSR
                     ILKKSGISSQILRLKKGVAPIVFGEVKSKKNPNKTLLFYNHYDVQPAEPFELWDDEPF
                     SGKVKGNKIFGRGSADDKGELITRIKAVESFLKNTGDVPCNIKFVIEGEEEIGSANIE
                     TFLKKYKRKFACDAVIWEFGYVDSKNRPIIGLGMKGLLYVELLAQESIRDAHSSLAVI
                     IKNPAWRLVDALKTLRDGSGRILIKDWYKEVVPFRGVDLKLLNKEPFDENDFKAEYGI
                     KEFVNGKKGIAVKKALAGDPTCNIAGFVSGYVLQGAKTVLPSSALVKIDFRLVPKMDP
                     KKQFARLRNHLRQKGFSDIKVKVYHGEAASRTNPNDPFVVDVKKSAKESFGNYVINVS
                     NAGTGPMHSFVNVLKSPCVSIGSTYIFSRIHSPNEFARIDLLKKTTKCMCLIIDKFSK
                     R"
     gene            5159..5740
                     /locus_tag="VGA92_00615"
     CDS             5159..5740
                     /locus_tag="VGA92_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX9844946.1"
                     /translation="MPGATAVGITFDGGVVFASEKRIAYGNFVVSKTTKKTFQLTDKV
                     GATIAGLVADMQILVLQIKALAKVRKMELKRDIPPNSIAKMMSNLMYERRFFPFLTQI
                     IVGGIVDKPTIYTLDPLGSVLPDEYAAVGTGAESALGVLDQQFKAGMKEKEAVDLAIK
                     SVKAATMRDSFSGDGIDVLVITKEGTKEFTEKL"
     gene            complement(5737..6213)
                     /locus_tag="VGA92_00620"
     CDS             complement(5737..6213)
                     /locus_tag="VGA92_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEX9844947.1"
                     /translation="MKSNLISKTDTAIVLKEISEQWRLELPKIKNLKIYELEDNARII
                     AGDGITALKVDQTYLPFLTDVKILERFPNVTVDMGAVKFMCDGANVMRPGIRSFSEFE
                     KGQLVCVVEESQKKFLAVGRALVPSSELQTMTKGVVIENLHYVSDKYWEIKKSVKD"
BASE COUNT         2092 a         1201 c         1366 g         1637 t
ORIGIN      
        1 taaagacaat cggaaaattt gatctaaatg tgcaggatgc tgcaaacaac ctctcaccaa
       61 aagtaattgc aaaatattgt tataatctgg cagttatgtt taacgcattt tatgaacatg
      121 tacgggttct ggatatcaaa gatgtgagtc ttcttaacgc cagattgtgc ctggtgcact
      181 ctttcaaatc ctgtcttgaa gaagcgctgg gtctaattgg cattgaaact cctacacgaa
      241 tgtagctacc caacgtttgt ttaaagtaaa caaactaggg ataatattga attattttaa
      301 aaaaaatcat aatagatggc tcaatttggt aggtcccaag catatagtca atgtgaattt
      361 tgcaatcaag tcgtagagat aacgggcgga actgtgcttt atggcggaaa atggtatcat
      421 aaacaatgtt cggaatttct gagctcaagg ggtggttgat ttggtaaaag taatagtagt
      481 tgatgatgat gtagatacag tcgaagtatt ttgtgagtat ttagaaatta aggacataac
      541 tgttttggac cgcggttaca atggaaagac agcagttgaa ctctatgaaa gattcaaacc
      601 cgatattgca ttattagatg tgatgatgcc agaatatgat ggattctatg gactagaaaa
      661 cattaaaaaa attaacccaa atgcaaaggt aatcatggtt acagctgatt tgacatatga
      721 tacagaaaaa caactaaagg cgctaaatgc ttctgcagta atctacaagc catatgaaat
      781 tgacagtgtc attgacacca ttcacaaagt gcacaaaggg gcagtcctac tccaacaata
      841 agcgcaaaaa acgctatatt tgtgaataaa cccatacaaa acatgaaaat actgatagct
      901 gacgacgagc cagatttact ttctcagttc cgggcagttc ttgtggatag gggccatacg
      961 gttgttacta ctggtgatgg tgatgcatgc gttatggcat actcaaagga atatgcagca
     1021 aacccggaaa gtccctttga tgttgtggtt cttgattatt ctatgccaaa aatgaatggc
     1081 cttgacgcgg caaaggagat tcttgggatt gcaccgtcac aacgaataat ctttgcatcc
     1141 gcctatgtgc aagaaacact cgtagattcg attaaaaacc ttaaacaaat tgtagagttg
     1201 ctgcaaaaac cattcagctt acaggatttg attgatacca tagaagacaa gacaatctat
     1261 gaagatttgg caaaactaaa tgtcaacata cagaacctca gggatctaaa cccgacacat
     1321 gcccaaattc gagattatct tgacgcacta aggaaactgc aaaaagcaaa aacgttctaa
     1381 gatgtataga ttcttatccg aaagatccaa gccacaatca tgactaagat aaagaataac
     1441 tcgtacgttt tatttttcta taatgactca aagaaatggt taaacaaaat ctcaaaagac
     1501 caagcactgc atactcatgt gggcgtcata aagcatagtg atgcgcttgg taaggaatat
     1561 ggctctcggg ttcgcacaaa taaggacaag tatgtctatc ttttggagcc gacaattcat
     1621 gattttgtga tgaaaagtca gcatgggacg cagatcgttt atccaaagga tctaggatat
     1681 attgctgcaa gggctggagt gcaaagcggt cagacagttg tggagatagg aactggtagt
     1741 ggggcactca ctacgttttt ggcaagcata gtaaagcctc gtggccacgt ctacaccttt
     1801 gatgttgagc caaaattcat ggaaattgcc aaaaaaaaca tcacacgggc aggcatgatc
     1861 aagtatgtga ccatggagaa tctggatatt aagactgcaa aaaaaatgcc cctcaccgag
     1921 gcagacattg cgataataga cttgggcgac ccttggagtg ttgtgccgca ggtgcgaaag
     1981 atgctaaagg gaagcggggc agttatagca atatgtccaa caatgaacca acttgagaaa
     2041 cttacaggct cgttagttga aaatgagttt actgacattg agtgcagcga acacattttg
     2101 cgaacaatcg acgcaagaga aggaaaaacc agacactctt tttatgggat tgggcacacg
     2161 acatacattg cgtttgcaag aaaggccttt tttgacagaa aaatcagaaa ggataatgac
     2221 gcctagattg cgttgtttgt acgtaacaca caaacataat ctaatagtct ttttatggtt
     2281 gtaaaagact gttgcggttt gctaagtgaa tcaaaaaaac cagaaaaata tgttgcatca
     2341 cagatcaata aggcacgtaa aagtctagaa acttttggaa ctgtcagtcc tgaaaaccac
     2401 ataacaaaag aagatttaca aaaacttggg cttgcagacg agattcgaaa actaaaatcg
     2461 attcaacgcg aaaaattact agaggatctc tccaccttgc tcaatgacaa gatacgcgaa
     2521 tttgaaaaac atgagcagga aatgaaatgg aaagactcta tgcttcaaag gttaaacaca
     2581 gaattgagcg aaaagataac acaacttgaa aaaacgaaca agcagctagt ggaagaaaaa
     2641 gcccgttccg acgagctaaa taggcagtta caggaaacat tgctcaagct tcacagctca
     2701 gaagaacaac tcaagctgga acgagactgg ttagccgagc aggtcgagac aaagtcaaaa
     2761 gaagtcctag aaacgattag ggaaatgatc aaagatgcag aaacccagaa taaaactgct
     2821 taaaaccaaa tagaagaaaa tttatccaca caacattcat cctacaacgt ggcccaaaaa
     2881 ctccaatccg cactgatcaa aaaattcatt cctgcaagaa agccagactc gcgcaaagga
     2941 caaaatggca aggtcctagt cgtgggaggg agctacatgt atcatggcgc tccggtgctt
     3001 tcctcacttt ctgcgttgcg ttcaggcgta gacttggttt ataccgcggt cccaaaaata
     3061 atagcgcctt ccgttagggc aatttctgca aacctgatag tgattccagt agttgactcc
     3121 aagctaacta gaggtgctac aaacaaattg cttggacagc ttccaactga tttggattct
     3181 gcaacaattg gtatggggct tgccattgcc gatgaagaag cactcaaaaa cctagtaagg
     3241 tcgttgatct cacaagatgt tagactgtca cttgatgcaa gcgcgttggt aagctacatt
     3301 ctaccattta tttcagaaaa aaacatagtt gtgactccac acgcaggaga attcaaaaga
     3361 atgtttggaa ttatgccatc agcctcaatt aaagaaaggg taaaaacagt ggaaaaactt
     3421 gcaaaagaac actcggtgac tattcttttg aaggcaagca ccgacgtaat atcaaacgga
     3481 aagcaaacct ttgtcaatcc aaaaaatctt gcagcaatga ctgttggggg gacgggtgat
     3541 gtgttgtctg gtttggttgc agggcttctt gcaaaaaatc gtaatccgct agaatcggct
     3601 gctgcagcat catacattaa tggccttgca ggtaagctgg tacaaagaaa aaacgggctg
     3661 catatggttg caacggattt gattgatact attccaacag ttatgaaacc gtttgataaa
     3721 gtgacaagat gaagaaaatt ctaacgcatg tggacaagaa tttcggcagc ctaatttctg
     3781 atcttcaagt gctgataaga cagccaagcg tttctgcaaa aaaccaagga atagaagaat
     3841 gtgctttgct tgtaagtcga attctcaaaa aatctggcat atcctcccaa atcttacgtc
     3901 taaaaaaagg agtcgcacca atagtctttg gggaagtaaa atcaaagaaa aatccaaaca
     3961 agacgttatt gttttacaat cattatgatg ttcaaccagc ggagccgttt gagctgtggg
     4021 atgatgaacc gtttagcggc aaagttaagg gaaacaaaat ttttggaagg ggctcagcgg
     4081 atgacaaggg cgagctaatc acgcgaataa aagcagtaga gtcttttcta aaaaacacgg
     4141 gggatgttcc atgcaacatc aagttcgtaa ttgagggtga ggaggaaatc ggaagcgcca
     4201 atatcgaaac atttctaaaa aaatacaaaa gaaagtttgc gtgcgatgcg gtgatctggg
     4261 aatttggcta tgtggactct aaaaaccgcc caataattgg gcttggaatg aagggtcttc
     4321 tttatgttga gcttttggca caggagtcta tcagagatgc gcactctagc cttgctgtga
     4381 tcatcaaaaa tcctgcatgg cggctagttg atgcactaaa aacactgcga gatggctctg
     4441 gtcgaattct catcaaagac tggtacaaag aggttgttcc atttcgtggg gtggacttga
     4501 aactcctaaa caaggagcca tttgacgaaa atgacttcaa agcagagtat ggaatcaaag
     4561 agtttgtcaa cggcaaaaaa ggcatcgcag taaaaaaagc actggcagga gacccaacat
     4621 gcaatatcgc tggttttgtg tcaggctatg tactgcaagg cgcaaaaact gttctacctt
     4681 catcagcact tgtaaagatt gattttagac ttgttccaaa gatggatcca aaaaaacaat
     4741 ttgcacgact caggaatcat cttaggcaaa aagggttctc agatatcaaa gtaaaggtgt
     4801 atcatggcga ggctgcctca agaaccaacc ccaacgatcc atttgtggtg gatgtaaaaa
     4861 agtcagccaa ggaatccttt ggcaactatg tgataaatgt ctcaaatgcg ggaactggac
     4921 caatgcattc ttttgtaaat gtactaaagt caccatgcgt ctcaattggc tctacataca
     4981 ttttttcaag aattcactca ccaaacgagt ttgcaagaat agatctgcta aaaaagacaa
     5041 ccaaatgcat gtgcctgatt atcgacaagt tttcaaaaag ataatacgtc tacgcatgtt
     5101 gtgtcagaaa agactttaat gtacatcaaa gggttgtgaa attatggctg caatgtatat
     5161 gcctggtgca accgccgtag ggattacctt tgatggcggc gttgtatttg caagcgaaaa
     5221 aagaatcgca tacggtaact ttgtggtaag caagactaca aagaagacat ttcagcttac
     5281 cgacaaggtg ggtgccacaa tagccggtct agttgcagac atgcagatcc tagtgttgca
     5341 gataaaggcc ttggcaaagg ttcgtaaaat ggagctaaaa cgagacattc ctccaaactc
     5401 gatagcaaaa atgatgtcaa atttgatgta tgagcgaaga ttctttccat ttctaaccca
     5461 aatcatcgtt ggtgggattg ttgacaaacc tacgatctat acgctagacc cattaggctc
     5521 tgttcttcca gatgagtatg cagcagtggg aactggagca gaatctgcac ttggagtttt
     5581 agaccagcaa ttcaaggcag gtatgaaaga aaaagaggca gttgatcttg ccataaaatc
     5641 agtaaaggcg gcaactatga gagattcctt tagcggggac ggaatcgacg ttctagtaat
     5701 aacaaaagaa ggaacaaaag agtttacaga aaaactctag tcttttactg attttttaat
     5761 ttcccagtat ttgtctgata cataatgaag gttttctatt acgacacctt tagtcatagt
     5821 ctgaagttca ctgcttggta caagcgctct tccaactgcg aggaattttt tttgtgattc
     5881 ctccacaaca cagacaagct ggcctttttc aaattctgaa aagcttcgaa tcccaggcct
     5941 cattacgttt gccccatcac acatgaattt tactgcgccc atgtctactg tgacgtttgg
     6001 gaacctttct agaattttaa catctgtcag aaagggcaag taggtctgat ctacctttaa
     6061 tgcagtgatg ccatcccctg ctatgattct tgcattgtcc tcaagctcgt aaatctttag
     6121 gttttttatt tttggcagct cgagtctcca ctgctcgcta atctctttta gtactatcgc
     6181 agtgtctgtt tttgagatta gatttgattt caatcccgta gtatttcctt acgaatatcc
     6241 agtagatctc ttagaatgga gcttgcagtt tcggttccac ccgctccctt accaat
//
LOCUS       DASWKH010000006         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_383092, whole genome shotgun sequence.
ACCESSION   DASWKH010000006 DASWKH010000000
VERSION     DASWKH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383092"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..252
                     /locus_tag="VGA92_00625"
     CDS             49..252
                     /locus_tag="VGA92_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844948.1"
                     /translation="MNIYDKRQISCTKCGKCIGEIDYDAEVVNPRCAHCANPLPDGFD
                     KLSYSVQRIKNMPDKKVISSVLT"
     gene            complement(261..1082)
                     /gene="nadC"
                     /locus_tag="VGA92_00630"
     CDS             complement(261..1082)
                     /gene="nadC"
                     /locus_tag="VGA92_00630"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX9844949.1"
                     /translation="MDIKKELARFLSEDIGRGDITASILTNKRISAKIVSRQNGVVAG
                     TQLAKEIFSMKGCSAKMTKKDGSSIKPNQTVLRVSGPARSILSCERTALNLLSRMSGI
                     ATQTSELVRKIKKVNPKTDLYSTRKTAPGLRYFDKEAVVIGGGKKHRMTLGDMIMIKD
                     NHIASEGSLVKLIQKAKKRKKTFEVEVETLADAVLAATLGVPIIMLDNFTPDRIRKTI
                     TTFKKLGLRSKVRLEASGGINSQNIKEFAKTGVDMISVGFITNSAKAIDFSLEVD"
     gene            complement(1150..>1711)
                     /locus_tag="VGA92_00635"
     CDS             complement(1150..>1711)
                     /locus_tag="VGA92_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9844950.1"
                     /translation="LDRAIYRMTHQLEDVMDYVRMPQLKKKDYSISLIFQDVMERVEV
                     PPTITIHPPLNDHTVFCDPEKMEIVFVNLLVNAIQAIGDKKGDVTVEIVDESSDENVV
                     IKVIDTGAGIQEEIMGKIFDPLFTTKQVGTGLGLSSCKSIVDQHGGTISASSKVGRGT
                     TFTIKMPKKSDWSAISKEKTQNSLIV"
BASE COUNT          418 a          381 c          332 g          580 t
ORIGIN      
        1 tacacaagat taattagatt tgttacacaa atatttactt gagcgccttt gaatatctac
       61 gacaaaagac aaatcagttg cacaaaatgc ggaaaatgca ttggagaaat tgattatgat
      121 gccgaagttg tcaacccaag atgtgctcac tgcgcaaatc ctctacctga cggctttgat
      181 aagctctcct acagcgtgca acgaataaaa aatatgcctg ataagaaggt aatttccagc
      241 gtactcactt agatttgaga ctagtcaact tcaagactaa agtcaattgc tttagcagag
      301 tttgtgatga acccaactga gatcatgtcg acgccggttt tggcaaattc tttgatgttt
      361 tgagagttga tgccacctga cgcctccagt cttactttgc tgcgcaatcc aagttttttg
      421 aacgtcgtta tggtttttcg tattctgtct ggagtaaaat tatcaagcat aattattggc
      481 acccccagtg ttgctgctaa aaccgcatct gctagagtct ctacttcgac ttcgaatgtt
      541 ttttttcttt tctttgcctt ttggattagt ttgactagtg aaccctctga tgcaatatgg
      601 ttgtccttta tcattatcat gtcccccaac gtcattctgt gtttttttcc acctccaatt
      661 actactgctt ccttgtcaaa gtagcgcaga ccaggtgcag tcttgcgcgt ggaataaaga
      721 tctgtttttg gatttacttt ttttattttt ctgacaagct cagatgtctg cgtggcaata
      781 ccgctcatcc ttgagagcaa gtttagtgct gttcgctcgc agcttaggat tgatcttgca
      841 gggcctgaaa ctctgagtac tgtctggttt ggtttgattg atgagccgtc ctttttcgtc
      901 attttggcag aacaaccctt catggaaaag atctcttttg caagttgcgt ccctgccaca
      961 accccatttt gtcgtgaaac tattttggca gaaattcttt tgtttgtcag aatgcttgcg
     1021 gtaatgtcgc ctcttccgat gtcttctgat aaaaatctgg caagctcttt ttttatatcc
     1081 atttacagaa ctggtttttt gtgcctaaat agaacctttt ctatggccaa actagtccgc
     1141 atacgcgctt tatacaatta aagaattttg cgtcttttct tttgaaatcg cagaccagtc
     1201 agattttttt ggcatcttga tggtaaatgt ggtgcccctg ccaaccttgc ttgaggcgct
     1261 tatcgtacct ccgtgctgat ctacaatgct tttgcagctt gacagcccaa ggccggttcc
     1321 gacctgcttt gttgtaaaca acggatcaaa aattttcccc atgatttctt cttgtatgcc
     1381 agccccagtg tcaatcacct taattaccac attttcatct gatgattcat ctactatctc
     1441 tactgttacg tctccttttt tgtcaccaat tgcctgtatt gcgtttacaa gcaggttgac
     1501 aaaaacaatc tccatttttt cagggtcgca aaagactgtg tgatcgttta gcggaggatg
     1561 gattgtaatt gtcggcggca cctcgactcg ttccatcaca tcctgaaaaa tcagcgaaat
     1621 cgaataatcc ttctttttga gctgcggcat tctaacataa tccatgacgt cttcaagctg
     1681 gtgcgtcatt cgatatatgg cgcggtcaag t
//
LOCUS       DASWKH010000007        26598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2973355, whole genome shotgun sequence.
ACCESSION   DASWKH010000007 DASWKH010000000
VERSION     DASWKH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 26598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26598
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973355"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGA92_00640"
     CDS             <1..186
                     /locus_tag="VGA92_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9844951.1"
                     /translation="YQKNTTKEAQLLHQVDKLEMALQAAEYEKSGFTKAQIASFIESA
                     KIAVTDSDLQKILSKFL"
     gene            221..454
                     /locus_tag="VGA92_00645"
     CDS             221..454
                     /locus_tag="VGA92_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX9844952.1"
                     /translation="MSEQDKDEMIRAQNDLIGVLFEIVKRLQANNTLDEEYFQIVSGK
                     QQRDVDKKRLEKILEERQENSKVVSRLLEKLQT"
     gene            complement(451..753)
                     /locus_tag="VGA92_00650"
     CDS             complement(451..753)
                     /locus_tag="VGA92_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844953.1"
                     /translation="MPLSNNIILKLNEITSQISNKKSLSDDDEHLIKDTFNKILENGE
                     NYDVDEIGSWFENEGSWNHKPVILRITNISHYVQTRFWQNPKKMRLISDHDDCGCD"
     gene            824..1171
                     /locus_tag="VGA92_00655"
     CDS             824..1171
                     /locus_tag="VGA92_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844954.1"
                     /translation="MQYFQALKMGQKRVQASREYLNKLTNNKAMPALALRDTKSDVWE
                     PVGEENYYAIVDESSGFVLTDTSGYIVSLCDKDGFSKAIVQGVTKEQRDQLVIEFQKD
                     SIPKFNGNVILPV"
     gene            1250..2476
                     /locus_tag="VGA92_00660"
     CDS             1250..2476
                     /locus_tag="VGA92_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX9844955.1"
                     /translation="MKYSTEVEVRGHLIDSLILTKIFDVIMDLGGEFEVLVMQVGKKK
                     KDPSYAKLLVQGKNQAQLDKILEQVYRVGATSTIGKEVALKAAPKDMVMPDNFYSTTN
                     NTTQILHKGKWITVENTMMDKCIVVKNGKARCVPIREVKKGDMIIVGEAGVKVTPPER
                     PREGSNVFEFMGSSSSSERPTQHIAKKVAQDIYKTKKQKGKIILVGGPAIVHTGAADS
                     VAELIRLGYIDGVLTGNALAVHDIEYATLGTSLGMNVHDGSLAVKGHRNHMDAINSVF
                     KSGSIEAMVKTKKLTRGIMYECVRKKIPFVLAGSIRDDGPLPDVITDVSVAQKKYKEV
                     LKDAKMVIMVSTMLHSIATGNMIPADVKVVVVDISQPTVTKLMDRGTWQALGIVTDVG
                     AFLPLVAQEIRKLGVL"
     gene            complement(2634..3044)
                     /locus_tag="VGA92_00665"
     CDS             complement(2634..3044)
                     /locus_tag="VGA92_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEX9844956.1"
                     /translation="MLRIVLDTNIMISAIIRNGKPRKLLEFGINGKYRILMSREILDE
                     FSEVLQRPKFKTTLDEVVSIVSALVESSENVPIKSNLQVIVSDPDDDIIINTAHDGKA
                     DYIVSGDPDLKDLKNFKGIKIMSVDEMLKILDGS"
     gene            complement(3049..3330)
                     /locus_tag="VGA92_00670"
     CDS             complement(3049..3330)
                     /locus_tag="VGA92_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9844957.1"
                     /translation="MLEEPEITTMSEKGQVVIPQNLREYLGIKPKTKLLVFVMEGNII
                     MKKMDMPDVKKEWNRIFQRMDKKGLKLDESDVTKEIKAYRKVKHKKTMG"
     gene            3409..3534
                     /locus_tag="VGA92_00675"
     CDS             3409..3534
                     /locus_tag="VGA92_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844958.1"
                     /translation="MQQNRHDNNKTHGYGNGQALGIVTDVGAFLPLVAQEIRKLH"
     gene            complement(3531..5456)
                     /locus_tag="VGA92_00680"
     CDS             complement(3531..5456)
                     /locus_tag="VGA92_00680"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9844959.1"
                     /translation="MTGFVFVPTKEQIENSNIFKFMQKHNIKSFKELHKKSVADLDWY
                     WNAVNEDIGIVWDKKYTIVSDFSKGKPWPKWFVDGKTNIINSGVDKFATKYPNQIAYC
                     FVSEDDTEKIITYSQLETDVNKLANALKALNVKKGDVVAVYMPMIFEAIVAILACAKI
                     GAVQTVIFSGYSTEALRIRLQDCGAKILFVSDGFTRKGKPVSQTKTVLDATRDTGVEK
                     IIVAPYKGVDRYYFSESVIDYSKLVANQSPECQAEIMDSEDPLFILYTSGTTGKPKGV
                     VHTHGSYSVYAGQQASYLIDLHSDDVLLWPADIGWITGLAWNVYGLLEVGATAIIYDG
                     AIDWPNPSRLWQMVQKYKVTIFGTSPTAVRMFRKYKIEPQKQFNLSSIRIIPTTGEPI
                     DEDSWWWLYEKVGNKKIPIMNLAGGTEIGGAMLSVFPGMKLKPTTVGMPCPGFDLDVI
                     DEKQKSLKNKKGFLVVRSPWPTMTRGLLNDDDRYTQTYWSQYKDVWFHGDYVLVDNDG
                     LWYMHGRVDDVINISGHRLSTVEIEQVAVVHQKISEAAAISIPDDMTGEAIALFVVLK
                     NKNDSDSIQKEITDHISEKIGKLAKPKLVCVISDMPKTRTGKIMRRLLKTKLLGEPLG
                     DLSALENPYVLDEIVPI"
     gene            complement(5492..5767)
                     /locus_tag="VGA92_00685"
     CDS             complement(5492..5767)
                     /locus_tag="VGA92_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844960.1"
                     /translation="MSNISNINADEAEIVRIISRLPEFAWLESADLSKIRSEIRHEIA
                     KILGEYFAEKTRNTKPNWTIKFKEAGISEDDGKTAISCARRLGIEIS"
     gene            complement(5764..7164)
                     /gene="gatB"
                     /locus_tag="VGA92_00690"
     CDS             complement(5764..7164)
                     /gene="gatB"
                     /locus_tag="VGA92_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9844961.1"
                     /translation="MIGLEIHCQLTNLESKLFCSCKANYRSFEPNTNICPVCIGLPGS
                     LPRLNKKAVEKATMIAMAFNCKTPQKLGFFRKNYFYPDLPKNFQITQINLYGPTSTGE
                     QGRISIEGKDIRIRRIQLEEDPGRLIYEGASERTQITLVDYNRAGTPLVEIVTEPDFE
                     DARQVRIFLNILSDLLENLGVSDPTLEGAMRADANVSIEGGAKVEVKNIGSFYDLEKA
                     IDFELIRQKSLKERGIQIVQETRHWDDRRKITISARTKEADEDYRYILEGDIPWVIMD
                     SQSLEKWKTTMPESISSKKDRYSGYGIPAQVADILSSDKYYSDLFEKAHNESNAKEIA
                     NMITTDLMGLVDTRERREKSKLDPKHLSDLVELIQSGKVTRSSAKNALQEIVKTGKPV
                     SEIVSQIGLGKISDQSEITKIIDEIITAEPAAAKQAKENPQTINYLVGKVMQKTKGRA
                     DPTMTLKLLKEKIASL"
     gene            complement(7161..8609)
                     /gene="gatA"
                     /locus_tag="VGA92_00695"
     CDS             complement(7161..8609)
                     /gene="gatA"
                     /locus_tag="VGA92_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX9844962.1"
                     /translation="MNLATSSIDYINNVKNGIITAEEFVAKTLEQISKHDKNLHAFIS
                     INENALDQARQIDRKIKSKEKVGAFYGMPISVKDNICVKGGKTTCASKMLQDFVAPYD
                     ATVISKLKSQDATIIGKTNLDEFAMGVATEFSAFGPSRNPWNQEYVPGGSSGGSAVSI
                     SALECVLSLGSDTGGSVRNPASFCSVVGLKPTYGLVSRYGLVSYANTIEQIGPMGRKV
                     EDTALLLNHIAGKDPNDNTSIDNGSQDYLEGIDAGIQGKKIGIIKEMSKGEGLDKNVE
                     SSFWRAASVFEKLGAKVDEVSLDMVSYTIAAYYVLVSAEAGSNLARYDNIRYGYDFDS
                     EGYEFNSYVSKARKNFGPEVTRRMILGGFVPSAGFAGKYLLKALKVKSKLTKEIQEAF
                     KKFDFLISPTVPILPFKFGEKINDPLAMYLADTNTATANLTNVPAISVPFEIHNGLPI
                     GIQIHANILQEKQLLQAAYALQSTTNIPGVPL"
     gene            complement(8611..8877)
                     /locus_tag="VGA92_00700"
     CDS             complement(8611..8877)
                     /locus_tag="VGA92_00700"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9844963.1"
                     /translation="MVTKEDIEHVAKLMRIDIDNTDQYFEQIQKILNYFDILDKANIE
                     SEEISVQEVSINDLRDDKYTPFESKLIDQLKNYKGSYIRAPKMN"
     gene            complement(8886..10196)
                     /gene="aspS"
                     /locus_tag="VGA92_00705"
     CDS             complement(8886..10196)
                     /gene="aspS"
                     /locus_tag="VGA92_00705"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX9844964.1"
                     /translation="MIGTDLEDWRRTHYSNQLSPQMADTDVTVMGWVLSVRGHGNISF
                     MMLKDMKDEIQIVAKAGSCPDEIRERITQLKPHSSVGIRGTIRPSEKAPGGIEIIPKE
                     MKVFSEVEKIPPFEPYAKTVKNIDTRLEVRPLDLKRKSLQHVFKARSLVLKSIRSYFY
                     ESEFLEINTPKMIATATEGGAALFPIFYYNKEAFLAQSPQLYKEQLTMSFEKVFEIAP
                     IFRAEPSRTNRHLSEAISIDVEEAYVDYNDIMARIEQIIKLSIKTIQDYSKENPDSEF
                     VVPEMPSKIPQYSYSDLVERMQKAGAKTEWGDDLYPSNLKKIGLNGFYFIKDWPTGPK
                     PFYVKVSKQDPKISESFDLMFGDLELSSGSTRVEKRSELEERMKNKGLKTDAFEYHLR
                     AFDYGVPPHAGCGIGLERLMMALTNTENIRDTTFYPRDVDRLTP"
     gene            complement(10277..11656)
                     /locus_tag="VGA92_00710"
     CDS             complement(10277..11656)
                     /locus_tag="VGA92_00710"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9844965.1"
                     /translation="MQATQVNQTKILILGGGFAGISVLRHLQKIIKKNKQLKITLVNE
                     DNFFLFTPMLQEIASGMLHPSSISIPLRVFSKDSEFIQAKVSSVDLKNKLVAITRKFD
                     GKVKVLDYDYLIIAIGSTTNFFGNKRIEKNSFTIKTIQDAIAIKTHLINMMEIADNEE
                     NQAIQEKILTVAVVGAGFAGVEVISELNDFVKDSIKSYYHNIDPANVKMILVSAKAGI
                     LPELGEGLAQKAYESLEKAGIRIIPNTKAVDAGEDFVFLGNGETIPCATLIWTAGVTI
                     DSVITSLECEHNGGRIVVDSYLRMPSYDNVFALGDCAAITDTKTGSLYPPTAQHALRE
                     SKIVTHNLKCAITKNGSQKPFNFESKGMMAVIGKGNGIAKIFGYDLSGIPAWFVWRTY
                     YLTMLPTFEKKLKVAIDWTVNLFFRRDITLVRKIKRKSLNKFDLDDISSLDVMLFKKE
                     EPAPDQKVT"
     gene            11972..12199
                     /locus_tag="VGA92_00715"
     CDS             11972..12199
                     /locus_tag="VGA92_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844966.1"
                     /translation="MSDNSDLVDYIKETRLILADSLDVLLNLEHEALYEKDEELVPIN
                     NVVELVDMSLDVSHSEKLKQAESELLKRQPI"
     gene            complement(12196..12996)
                     /locus_tag="VGA92_00720"
     CDS             complement(12196..12996)
                     /locus_tag="VGA92_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844967.1"
                     /translation="MTKTKRFHGYSTDIERLATRIEAYLSENNFEVAFSKDQTTPVST
                     FFIQARKLGMLRTAAGIRRSTDIKIQGTPENFEVTIGTGQWGNNLIVSAPLFVIPVMG
                     IVATVARLYTAKKFESNLWKYIKEQASFLRNSAESNKTEVAKPLDRREYKCDYVDGYP
                     GWNSQVLDGKIILQKQKSGFDRIIFESPDGEQFTIPATKIEKATILSRKKGLAENDLM
                     LEITCKDKNGNTINPVLNLSDDVIAGVLAGINEFVAEDQQLRSLYKQH"
     gene            13306..13695
                     /locus_tag="VGA92_00725"
     CDS             13306..13695
                     /locus_tag="VGA92_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX9844968.1"
                     /translation="MEISFDEISDLRSFINNLNYVQESIVVVEGKRDSEALKKLGFTE
                     QVLEFHSFGGITKFADSVAHHKNLIILFDSDRKGKYLTRRIIEQLERRVRIDLSHKKR
                     LVEITKGKIRAIEELIRYESPVNDYII"
     gene            13801..14751
                     /locus_tag="VGA92_00730"
     CDS             13801..14751
                     /locus_tag="VGA92_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX9844969.1"
                     /translation="MVLFIDGHVTSKASLEKIKGIVNGDVDEFGVSTIELFYNNKAQE
                     MYCILDAPDEGSVVKHHAKIGLTCNFITQIEKIHTKTADNTARLVAIGELAARLAHDL
                     RNPLSVIKNTVEIMESKPKFRIEDRVIYYNRLHRAVDRISHQIEDVLDFVRPLTLTFE
                     NHLINDIIASAIEKITVPDSVRITLPNNFVYLACDFTKLEVVFTNLIINAIQAMNNVG
                     QVDIKIVDLDKHVVIQIADTGCGIPQNIMSKIFEPLFTTKQTGTGLGLASCKKIIEQH
                     DGAIEISSTEGKETIFTIKLPKRNIVPSQEKQLEKNTLVS"
     gene            complement(14776..15564)
                     /locus_tag="VGA92_00735"
     CDS             complement(14776..15564)
                     /locus_tag="VGA92_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550416.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9844970.1"
                     /translation="MNKTITIAAATGIVLSLAFVLGAIPSGIVAQAQTEEPTPFPSRE
                     KTISVTGTATTSVDPDLLVVQFGVEVQETTAKEALDANSQLMTAVVEAIRAAGITDNE
                     INTAQLTIYPVYESYQEKETGIYRQKLVGYAVSNIIRVETKNLSLASDIIDSGVSAGA
                     NRVDSVYYTLSPQRQMQVSDDLLSEAIINAKSKAEKALAPLDYQIIGVKHVSLSDFGY
                     PPPPIPYYKGAFDAMAESAIRTPVFSSDQDVSTTASVVFIIGSN"
     gene            complement(15689..15904)
                     /locus_tag="VGA92_00740"
     CDS             complement(15689..15904)
                     /locus_tag="VGA92_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEX9844971.1"
                     /translation="MERIRVDSGNIRIIGYDESTQTLEIEFQQSGTYQYFGISKKIHD
                     DFMKYALSHNQYYTRYIKNRYRHKKIR"
     gene            complement(15939..16076)
                     /locus_tag="VGA92_00745"
     CDS             complement(15939..16076)
                     /locus_tag="VGA92_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844972.1"
                     /translation="MTQPKTAPIRQKNKNNKTKPENLMKKEYLEDFDELKKSIIENQK
                     T"
     gene            16483..16833
                     /locus_tag="VGA92_00750"
     CDS             16483..16833
                     /locus_tag="VGA92_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844973.1"
                     /translation="MTKVGIELFVIINNSGRIIDSFGNKDLSISDEKQRLLLMQMALQ
                     SSMQGDYNEEFGKVRSCIVQRNKLKFICCPMGEDKTALVVANKTIPDIDIINHINQLA
                     NDTYIVAEGATLLV"
     gene            16830..17021
                     /locus_tag="VGA92_00755"
     CDS             16830..17021
                     /locus_tag="VGA92_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844974.1"
                     /translation="MNLYDLKQLHCHKCGKCIGEIHYDAEITMPLCGPCANPMPEGDD
                     KLLYTASKYGRSLVNTISA"
     gene            17186..17875
                     /locus_tag="VGA92_00760"
     CDS             17186..17875
                     /locus_tag="VGA92_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9844975.1"
                     /translation="MGDSEDPMEINDKVEILSTDDDKIKSVGELLSSDSSRAILKLLF
                     NDQLTANQIAQKTEISLPLVIYHLKKMQETGMVKISQTGKNTKSHDMKYYTVDKFAIV
                     ILPSGATEKAKSSKSLFNSFNRIYRFSMIGGAAIAAWFSSQFLQIQQASLPTESMRGA
                     DVMMEQAEEAAEKSLQAPAPSITESVPFAAQGDIQTGEPATDIYISIIVTLGVVVLGL
                     IIERVLRAQRK"
     gene            17907..18125
                     /locus_tag="VGA92_00765"
     CDS             17907..18125
                     /locus_tag="VGA92_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844976.1"
                     /translation="MALTNREKALVVISNAISVYSIYTKNPDMLPKNISLIDFVLKST
                     PVELKKEISMDLIDEIFDFVSKTQMELS"
     gene            18156..19181
                     /locus_tag="VGA92_00770"
     CDS             18156..19181
                     /locus_tag="VGA92_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase"
                     /protein_id="HEX9844977.1"
                     /translation="MASIATLGSHCALQVLKGAKDEGFKTLLVCEKKRENLYRRFKFI
                     DELVLVDSFLELLDSKCASILEKNKAVLIPHGTLIAQMSTEQIESIKTPIFGNKWILK
                     WESDRLLKEKLMLEANLDIPASISDPKQIKKLTIAKRHGAAGGKGYFLTTNEADYIKK
                     RDRLVSMGLIKGDSDLYLQEYVMGVLAYLQFFYSPLKDELEFFGVDKRHESDIEGLAR
                     IPAQEQLGTDYISSFNVIGNSPMVLRESLLQDVYDMGQKFVDASKRLVSPGMNGPFCI
                     EGVYDQDGKFWSFEFSARIVAGTNIYMDGSPYSTLLYDVPMSMGRRIAREVKNAEKAK
                     KLDKITT"
     gene            complement(19174..19647)
                     /locus_tag="VGA92_00775"
     CDS             complement(19174..19647)
                     /locus_tag="VGA92_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844978.1"
                     /translation="MKNYLFVIVSTVMLSIIPLSLADQGTYKLEVDENTFDVLYSFDG
                     ELLAMTIDQESTSLLIGTQNVEDSFFEVSFSSELLSAQNAEFVVLVDGLEADYTITYD
                     GDNPALTFPIPADSEEIEIIGTSVIPEFPLGALAIMGAVSATVIILTRMRQRLFK"
     gene            complement(19755..20369)
                     /locus_tag="VGA92_00780"
     CDS             complement(19755..20369)
                     /locus_tag="VGA92_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844979.1"
                     /translation="MKTTIMMTVMAAAIAISTIALVVSFSQTEEIVAAESPQGNDAYL
                     FGESVYPLVTFKFRETTVTYPFQTFVQLNGLFGTSGSFTTKSVAPEFTLQRVAGDTPF
                     LHRAVDQTYVHGGKNSLGEYAYKEFGITVDLVQEGKVVRSFDYGRCGVANYKVNTEFD
                     KAESFTGKDGFAVLEQYTFQCAGFEPKNPTYDELMKPKSIILDD"
     gene            20539..21504
                     /locus_tag="VGA92_00785"
     CDS             20539..21504
                     /locus_tag="VGA92_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9844980.1"
                     /translation="MNKLFAVIPIMLAVGIGIGFGIFSVANNADLESSQTPSKKIVTL
                     AIQPTAQASDIESQAKELEQFLEERTNYDVQIYVPTSYAGVVEALRFGKADMAFMSAW
                     PSYLAVQKADATLEIAEVREVVIGDTMTAETYYYSYWVVPKDSPYNTLEDLRGKRAAF
                     PSPLSTSGYVTPMKTLVETGLITVESGKEIDPKAYFSDVIFAGGYGQAWEALKNNKVD
                     VSIIAGDVSEKLYREVRDNTKVVHEQGPIPSHGVVFSKDMDAEAKQKVKDALLEMGKD
                     ESSKQIMKKLVSAIFVGFEETTADQHLGNLQDALQLTGLKYTEKV"
     gene            21515..22237
                     /locus_tag="VGA92_00790"
     CDS             21515..22237
                     /locus_tag="VGA92_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9844981.1"
                     /translation="MSDVLSMNQIQLIINERKILNNISMSVKQNSAVTIMGPNGAGKT
                     TLLKIALGLLQPTSGELYLFGKKGLDKQRRNLLGYIPQNLGLVNELSVFSNVMMGALR
                     RMPRWRAITNMYPKELLTEAYSLMYELKITHLKDIKIKKLSGGEKQRVAIARTLIQKP
                     SMILADEMTASLDFKAAVAVMDIFAEIRKKKGITLLMTHHNPDIAKIYSDTVFIMING
                     VIEKTMESKEMSEDTISRLYEA"
     gene            22296..22985
                     /locus_tag="VGA92_00795"
     CDS             22296..22985
                     /locus_tag="VGA92_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9844982.1"
                     /translation="MGFNPLSIIEDADNSLIIIQELLAFDFSVGDKVLFSLIETLEMA
                     LIGSSMGFVLSIPAALLAARNVSPPFISAIFRGILGILWSMPPLLWAILLVVIVGLGP
                     TAGIFAIALYIMGLSGKYLYEIYESQNLSAYDAMHVIGATRMQIAKFVTIPEALPHMS
                     NQYLFLLAYSVRESSILGLVGAGGIGFYIIHHLESLNYGKAAPFILGILAVTLGMDYI
                     STKLRKRLVYH"
     gene            23018..24166
                     /locus_tag="VGA92_00800"
     CDS             23018..24166
                     /locus_tag="VGA92_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844983.1"
                     /translation="MVIEYDKAMKKIFGLLIVVLITTGVLVQINPIFAPSHPVFAGQN
                     MLVFYGKGTTDQSSPMPDEVIRTVINKDTATIVHPAKGIEIIRMNIQTSDACIQTQAT
                     VCFDGIVTDTRNVDVHKIGDKIGITFDFQNKKQIISFDSGLMQGVSFTVDLSKTIIRL
                     DGPLTVTLAQEGGFAGVQNTFVVDMASAQLTKNDSIIMLDEDSISEITSLIKKIKLFD
                     VDEETYPPIEGSADYFTYFIQISHGQIQKTVSWTDTSEIIPEKMNALKDVIIKISSNI
                     QTSEPAPDILQAELAIDFVKNAPTFAFDGMGDSLTVEDVMILESFPVQYVVTINFESL
                     HGGYGDRTGQYLTQAITPHTIIVTVVDGQITSAIIDDIWDELSQETIE"
     gene            complement(24173..26287)
                     /locus_tag="VGA92_00805"
     CDS             complement(24173..26287)
                     /locus_tag="VGA92_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9844984.1"
                     /translation="MRRIRVGIDVGGTFTKAVAIDIKTGQIIGKSTVPTTHRAEKGVS
                     KGIVTALSNLLEESKIEISEIELIAHSTTQAINALLESDTAKVGIIAMGVTPEKGEVI
                     KRTNLHDTKFNSDQSLKTEHVFLDTSHLISESEVKRALTDLKNKGAQVIVATEAFGVD
                     DPSNEMFVMKNAMANQIPATASHEISGIYGLEIRTLTAAINASVLPKTFEVANFVEDA
                     IRNAGVTAPLMIMKGDGGVTSMDTFRTKPILTILSGPAASVAGALLYLKITNGIFIEV
                     GGTSTNICIIKNGKPEVRYVTIKDHPTCIRSMDVRILGVAGGSMVKLRDKRVWKVGPR
                     SAHIAGMRYACFAEPEVLKKGKLVLVRPKENDTESYAAIRCEGETFAITNTCAANALG
                     MIEKNDYSYANQNSAKIAMELLGREIGISGPEVAMSIIQTASFEITKAVSKILKEFKM
                     DKTKTQIIGGGGGASVLVPFVAKQLTIPYKKAEHAEVISSIGVASSMIQEEIEHTITK
                     PTPEIISDLHKKIHALMIDRGAVPESIVINSEFISEKSLLRVSATGNVELDSSENAKN
                     VFTLDEALIRAGEMMQINKDLVELSFETDHYFVFIGHVSEKKFFSKKNKHHILVLDRF
                     GRQKLSIKNGKLFQGGKISILEELDDFLESRRSDIAPQVYLLNNLKLIDFSSLTSASH
                     ILGAVQQELDSSEKAAVIIEIS"
     gene            complement(26274..>26598)
                     /locus_tag="VGA92_00810"
     CDS             complement(26274..>26598)
                     /locus_tag="VGA92_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HEX9844985.1"
                     /translation="RLPLKMLFGLTMGVGAYLSIAFLGNAIRELQTLDIVPYTSMLGI
                     IPRLDINMATMTGIYPTLETLVSQIILLGVYLVAASYVLILRPKREEKISAMRKSRSE
                     IDETH"
BASE COUNT         7686 a         5265 c         5317 g         8330 t
ORIGIN      
        1 tatcaaaaaa acacaacaaa ggaagcacag ctgctacacc aagtagacaa gcttgagatg
       61 gcactgcagg ccgcagaata tgaaaagtct gggtttacaa aggcacagat tgcctcattc
      121 atcgagtcag caaaaattgc agtgacagat tctgacttgc aaaaaatact ctcaaaattt
      181 ttgtaaaact tacaaatagc tgtaacttac gcaaatcagg ttgtcagaac aggacaaaga
      241 tgagatgatt cgtgcgcaaa acgatctaat cggagtattg tttgaaatag ttaagagact
      301 tcaggcaaac aacacactcg atgaggaata ttttcagatt gtttctggta agcaacaacg
      361 agacgtagac aaaaaacgcc tagaaaaaat ccttgaagaa cgacaagaaa acagcaaggt
      421 tgtctcaaga ctgcttgaaa agctacaaac ctagtcgcag ccacaatcgt cgtgatctga
      481 tatcagtctc atcttttttg gattttgcca aaacctagtt tgtacatagt gtgagatgtt
      541 ggtgattcta agtattaccg gcttgtgatt ccagcttccc tcgttttcaa accagctccc
      601 gatctcatcg acgtcataat tttctccatt ttccagtatt ttgttgaagg tgtctttgat
      661 tagatgctca tcatcatctg agagagattt tttgtttgat atctgggatg taatctcgtt
      721 tagctttagt attatgttgt ttgagagtgg cacatcatcg tgtcaattct gacgcaatat
      781 aatgatgctg tacattttaa taattaaagc ataaaatcaa tttgtgcaat actttcaggc
      841 actcaaaatg gggcaaaaac gtgttcaggc atcaagagaa tatcttaaca aacttacaaa
      901 caacaaggca atgcctgcac ttgcactacg tgacacaaaa tcggatgttt gggaaccagt
      961 aggagaggag aactattacg ctattgtaga tgaatcaagc ggctttgtct taactgacac
     1021 aagcggatac atcgtgtcac tttgtgacaa agatggattt tcaaaagcaa tagtacaggg
     1081 tgtgacaaaa gaacaacgtg accaactagt aatagaattt caaaaagaca gtatcccgaa
     1141 attcaatggc aacgtaattc ttccagtata attgtaaaga aaaaacctag tcgtgtcaac
     1201 aattctacat ggcacatatt ttaaattccg tatgggtggt aatctaggaa tgaagtattc
     1261 aaccgaagtc gaggtacgcg gacacctaat tgattctctg atacttacaa aaatctttga
     1321 tgtgataatg gaccttggag gagagttcga agtccttgta atgcaggttg gaaaaaagaa
     1381 aaaggaccca agctatgcta agctcctagt tcagggcaaa aaccaggcgc aactcgataa
     1441 aattttagaa caagtctatc gtgttggtgc gacatcgaca ataggaaaag aggtggcact
     1501 caaagctgcg ccaaaagaca tggtcatgcc agataacttt tacagcacaa caaacaacac
     1561 gacacagatc cttcacaaag gaaaatggat tacagtagaa aacacgatga tggataagtg
     1621 cattgtagtc aaaaacggca aggcaagatg tgttcccatc cgtgaagtaa aaaaaggtga
     1681 catgataata gtcggtgagg caggcgttaa ggtaactccg cctgagcgac caagagaagg
     1741 ctccaacgta tttgagttta tgggaagcag tagctcaagt gagcgaccaa cacagcacat
     1801 tgcaaaaaaa gtggcacaag acatttacaa aacaaaaaag caaaaaggaa aaatcatatt
     1861 ggttggtggc cctgcaatag tgcatactgg tgcagcagat tctgttgccg agttgattag
     1921 gcttggttac atcgacggag tattgactgg aaacgcactg gctgttcacg acatagaata
     1981 cgctactctt ggaacttcgc ttggaatgaa tgtccatgat ggctcgcttg cagtcaaggg
     2041 gcacagaaat cacatggatg caattaactc ggttttcaaa tctggttcta ttgaagccat
     2101 ggtcaaaaca aaaaagctaa ctaggggaat aatgtacgaa tgtgtacgaa aaaagattcc
     2161 gtttgttttg gctggctcca ttcgagatga cggaccacta ccagatgtca taactgatgt
     2221 gtctgttgct caaaaaaaat acaaagaagt tctaaaggat gcaaaaatgg tcatcatggt
     2281 ttcaacgatg cttcattcta ttgcaactgg aaacatgatt ccagcagacg tcaaagtagt
     2341 agtagtcgat atcagccagc caacagtaac aaagctcatg gacaggggaa cgtggcaggc
     2401 cttgggaata gtgactgatg ttggcgcgtt tttgcccctt gttgcccaag aaataagaaa
     2461 actaggcgta ttgtagactc tggcttactg atgttgagaa ctagagtacg acatgattta
     2521 tctgtggata cgaattccgt tagccccgcc ttaactctgg gtgctaatcc ttttgcagca
     2581 cgaccttgcc gttaggaaac attccgacga aaatccatcc gctagaaatt atgctagctc
     2641 ccatctaata ttttcaacat ttcatctacc gacataatct taattccttt aaaatttttg
     2701 agatctttta gatctggatc tccagaaact atgtaatctg cctttccatc atgtgctgta
     2761 ttgattataa tgtcatcatc tggatcactg actatgactt gtaaatttga tttgatcggc
     2821 acattctcac ttgattcaac taatgcagaa acgatgctga cgacctcatc taatgtcgtc
     2881 ttgaattttg gtctctgaag aacttcagaa aattcatcca agatttccct tgacatcaag
     2941 atccgatatt tgccgttaat tccaaactcg agcagctttc taggttttcc gttacggatt
     3001 atagcactta tcatgatgtt ggtatcaagg actatcctta acacgggatc aacccattgt
     3061 tttcttgtgt tttaccttcc tatacgcttt gatctctttg gtcacgtcgc tttcgtctag
     3121 tttgagaccc ttcttgtcca tacgctggaa tattcgattc cattcttttt ttacgtctgg
     3181 catgtccatc tttttcatta tgatgttgcc ttccataacg aacacgagta gcttggtttt
     3241 cggctttatt ccaaggtatt cccttagatt ctgcggtatg actacctgcc ccttttcact
     3301 cattgttgta atctcgggtt cttctaacat tcctaccagt aagattactt tactttctta
     3361 ctatttatag tcattgtatt ttggttaaaa caggctacct accacatctt gcagcagaat
     3421 agacacgaca ataacaaaac tcatggatat gggaacgggc aggccttggg aattgtgact
     3481 gatgttggcg cgtttttgcc ccttgttgcc caagaaataa gaaaactgca ctaaatcggc
     3541 actatttcat caagtacgta tgggttttca agcgcgctta ggtctcctag cggctcccca
     3601 agaagcttcg ttttgagcag tcttctcatt atttttccag ttcttgtctt tggcatgtct
     3661 gatatgacac aaaccagttt gggctttgca agcttgccta ttttttctga tatgtgatct
     3721 gtgatttctt tttgaataga atcactgtcg tttttgtttt ttagcacaac aaaaagtgcg
     3781 atggcctcac cagtcatatc gtcagggatt gatattgcgg cagcctctga aattttttgg
     3841 tgcacgactg caacttgttc tatttccaca gtactaagcc tgtggcctga aatatttatg
     3901 acatcatcaa ctcggccatg catgtaccac aaaccatcat tgtcaactag gacatagtca
     3961 ccatggaacc agacatcctt gtattggctc caatatgtct gggtgtatct atcatcatca
     4021 tttaaaagtc ctcgagtcat cgtcggccac ggggatctaa ccactagaaa tccttttttg
     4081 ttttttagcg atttttgctt ctcatcgatt acatcaagat caaaaccagg gcacggcatg
     4141 cctactgtgg tgggttttag cttcattcca ggaaaaacag aaagcattgc accgcctatc
     4201 tcggttccac cagccaaatt cattattgga attttcttgt ttccaacctt ttcataaagc
     4261 caccaccatg aatcctcgtc gattggctcg ccagtcgtag gaattattct aattgaggag
     4321 aggttgaatt gtttttgtgg ctctattttg tattttctaa acatccttac tgctgttggt
     4381 gaggtgccaa aaatcgttac cttgtatttt tgtaccatct gccagagtct tgatgggttt
     4441 ggccagtcga ttgcgccgtc ataaattatc gcagttgcac caacctccaa aaggccatac
     4501 acgttccagg ccaaccctgt aatccagcca atgtctgctg gccagagcag aacatcgtca
     4561 gaatgtaggt cgattagata tgaagcctgc tgcccggcat atacagaata gctgccgtgt
     4621 gtatgaacca cgccctttgg cttgccagtc gtgccggatg tatacaaaat aaacaacgga
     4681 tcttcagagt ccatgatttc ggcttggcat tctggtgatt ggtttgcaac aagttttgag
     4741 taatcgatta cagattctga aaaataatac ctgtcaactc ctttgtatgg tgcgacaata
     4801 atcttttcaa caccggtgtc cctcgttgcg tcaaggactg tctttgtctg cgagactggt
     4861 tttcccttcc ttgtaaagcc gtctgaaaca aaaagtattt ttgcaccgca atcctgtagt
     4921 ctgatcctaa gtgcttctgt gctgtatcca gaaaaaatta ctgtttgtac agcaccaatc
     4981 tttgcacagg caagtatggc aactattgct tcaaaaatca ttggcatata gacggcaaca
     5041 acgtcacctt ttttgacatt taatgccttt agcgcgtttg caagcttgtt tacatcggtt
     5101 tccagctgtg aatacgtgat tattttttcc gtatcatctt ctgagacaaa acaatacgca
     5161 atttggtttg gatattttgt tgcaaattta tcgacacctg aatttatgat gtttgttttg
     5221 ccatcgacaa accacttggg ccaaggcttg cctttggaaa aatctgaaac aatggtgtat
     5281 tttttgtccc acactattcc aatgtcttca tttacagcgt tccaatacca atccaagtct
     5341 gcgaccgatt ttttgtgaag ttctttgaat gacttgatgt tgtgtttttg cataaatttg
     5401 aaaatattcg agttttctat ctgctctttt gtgggaacaa aaacaaagcc agtcaaacag
     5461 ttttctttga aattgtttta atttaaatga attaagaaat ttctattcct agccttcttg
     5521 cgcaggagat tgcggttttt ccatcgtctt ctgatatgcc agcctctttg aacttgatgg
     5581 tccagttggg tttggtattg cgcgtttttt ctgcaaaata ttcgccaaga attttggcta
     5641 tttcgtgcct gatttcactc ctaattttgg aaaggtcggc tgattctagc catgcaaact
     5701 cgggtaatct tgagattatt cttacaatct cggcttcgtc ggcattaatg ttggaaatat
     5761 tactcatagg gatgctatct tttctttgag aagttttagc gtcattgtag gatctgccct
     5821 tccttttgtt ttttgcatca ccttgccaac aagatagttt attgtctgtg ggttttcttt
     5881 tgcttgtttg gctgcagctg gttctgcagt tattatctca tctattattt ttgtaatctc
     5941 ggattgatca gatattttac caaggcctat ctgtgagaca atttctgata caggttttcc
     6001 agtcttgact atttcttgaa gtgcattttt tgccgagctt cgagtaactt tgcctgactg
     6061 aattagctca acaaggtcag agaggtgttt ggggtcaagc tttgatttct ctcttctttc
     6121 tcttgtgtca acaagaccca tcaaatctgt ggtaatcatg tttgcaatct cttttgcatt
     6181 tgattcgttg tgtgctttct caaacaaatc tgaataatat ttgtcagacg acaaaatgtc
     6241 tgccacttgg gccggtatgc catacccaga atacctgtct ttttttgagc taatgctttc
     6301 tggcatggtt gttttccatt tttccagcga ctgtgagtcc ataatgaccc acgggatgtc
     6361 gccttcgagg atatagcgat agtcctcgtc tgcttctttt gttctggcag atattgtaat
     6421 ttttcgtcta tcgtcccaat gcctcgtctc ttgcacgatt tgtattcctc tttctttgag
     6481 acttttctgt cttattagct caaaatcaat tgctttttcc aaatcataaa aagaaccaat
     6541 gtttttgacc tctactttgg ccccgccctc aatagacacg ttagcgtctg cgcgcattgc
     6601 accctccagt gtcggatcag atactcccaa attttcaagc aaatccgaca agatatttag
     6661 aaatattcta acctgccttg catcctcaaa gtctggctct gtcactattt ctacaagggg
     6721 cgtgcctgcg cggttgtaat ccacaagcgt aatttgtgta cgctctgagg cgccttcgta
     6781 gattagcctt cctgggtcct cctcaagctg gattctgcga attctgatgt cctttccctc
     6841 tatggatatc ctgccttgct cgccggtact tgttggccca tacaagttaa tctgggtaat
     6901 ttggaagttt tttggcagat caggataaaa ataatttttc ctaaaaaaac caagtttttg
     6961 tggggttttg cagttgaatg ccatggcaat catggttgcc ttttcgactg cctttttgtt
     7021 tagtcttggt aaagatcctg gcaatccaat acaaactgga caaatgtttg tgtttggttc
     7081 aaaactcctg taatttgcct tgcaagaaca aaacagcttg ctttcaagat tagttagctg
     7141 gcagtgaatc tcaagcccaa tcatagcggc acgcctggaa tatttgtggt actttgtagg
     7201 gcgtatgctg cttgcaataa ttgtttttct tgcaatatgt ttgcatgaat ctgtattcca
     7261 attggcaagc cgttatggat ctcaaaggga acggaaattg ccggcacatt cgtaaggttt
     7321 gcagtagctg tgtttgtgtc tgcaagatac atggcaagtg ggtcgttaat tttttctccg
     7381 aacttgaatg gcaaaattgg tactgtgggt gaaatcaaaa agtcaaattt tttgaatgct
     7441 tcttggatct cttttgttag cttgcttttt accttgagtg ccttgagaag atattttcct
     7501 gcaaaacctg cagatggtac aaaaccgcca agaatcattc ttcttgtaac ttctgggccg
     7561 aagttttttc gtgcctttga tacatatgag ttgaattcgt atccttctga atcaaaatca
     7621 tatccatatc ggatgttatc gtatcttgca aggttgcttc cggcttcagc tgacaccaaa
     7681 acatagtagg ctgcaatggt atagcttacc atatctagtg agacttcatc aactttggcg
     7741 ccgagttttt caaaaactga agccgctctc caaaatgatg attcgacatt tttgtcaagg
     7801 ccttcaccct tgctcatctc tttgattatg cctattttct taccttgtat tccggcatca
     7861 attccttcaa ggtaatcttg actgccatta tctattgagg tgttatcgtt tgggtctttt
     7921 cctgctatgt ggttcaaaag caaggctgtg tcctctactt tacgccccat tggaccaatc
     7981 tgctcaatgg tgtttgcgta tgatacaagg ccatatctgc tgactaggcc gtaggtgggt
     8041 tttaggccta caactgagca aaaactggcc gggtttcgaa cggaaccgcc agtatctgag
     8101 ccaagcgaca aaacacattc aagggcagaa atggaaactg cacttccacc agacgagccg
     8161 ccaggaacat attcttggtt ccaaggattt ctgcttggac caaaagcaga aaactctgtt
     8221 gccacaccca ttgcaaactc gtctaggttt gttttcccaa ttattgtggc gtcttgcgat
     8281 ttcaattttg atatgactgt tgcatcatat ggggctacaa aatcctggag catttttgat
     8341 gcgcatgtag tcttgccccc tttaacacag atgttgtctt taactgatat tggcatccca
     8401 taaaaggcgc caactttttc ttttgatttt atttttctat caatctgtct tgcttggtct
     8461 aaggcatttt cattaattga gataaatgca tgcagatttt tgtcatgctt tgatatctgt
     8521 tctaatgttt ttgcgacaaa ctcttctgcg gtgatgattc cattctttac attgttgata
     8581 tagtctattg aagatgttgc aagattcaat ttagttcatc tttggagctc taatgtagct
     8641 tcccttgtag ttttttagct gatcaattag ctttgattca aacggtgtgt atttgtcatc
     8701 cctaagatca tttattgata cttcttggac tgaaatctct tctgattcga tgtttgcctt
     8761 atctaggata tcaaaatagt tcaagatttt ttgaatttgt tcaaaatact ggtcagtgtt
     8821 gtctatgtca attctcatta attttgcaac gtgttctatg tcttcttttg taaccaagta
     8881 atcacctatg gagtaagtct gtctacatct cttgggtaaa atgtggtatc tcttatgttt
     8941 tcagtgtttg ttagtgccat catcagtctt tcaaggccaa ttccacagcc tgcgtgcgga
     9001 ggaaccccat agtcaaacgc ccttagatgg tactcaaagg catcggtttt gagcccttta
     9061 ttcttcattc tttcctccag ttctgagcgt ttttcgactc ttgtgctacc tgaagacagc
     9121 tcaaggtcgc caaacataag atcaaaggac tctgaaatct tggggtcttg cttgctgacc
     9181 ttgacgtaaa acggctttgg gccagtaggc caatccttga taaagtaaaa cccgttcaat
     9241 ccgatttttt taaggtttga tgggtataga tcatcgcccc attctgtctt tgctccagcc
     9301 ttttgcattc tttctacaag atcagaatag gaatactgtg gaattttgct tggcatctct
     9361 gggactacaa attcggaatc tggattttcc tttgaatagt cttgaattgt tttgattgac
     9421 agttttatga tttgctcgat tcgtgccatg atgtcattgt aatctacata cgcttcttcc
     9481 acatctattg agattgcctc agaaaggtgt ctgtttgttc ttgatggttc tgctctaaag
     9541 attggcgcaa tctcaaagac cttctcaaag ctcatggtga gctgttcttt gtacaactgt
     9601 ggtgattgtg ccaaaaatgc ctctttgtta tagtagaata ttggaaataa tgcagctcct
     9661 ccctctgtgg cagtagcaat catttttggg gtgtttatct ccaaaaattc actttcgtaa
     9721 aaataactcc taattgattt taggacaaga cttcgcgcct tgaagacgtg ttgtagagat
     9781 ttacgcttta agtcaagcgg cctgacctca agccgggtat caatgttttt gactgtcttt
     9841 gcatatggct caaatggcgg gattttttcc acttcggaaa aaaccttcat ttctttaggg
     9901 atgatctcta ttccccccgg tgctttttct gatggtctta tggttcccct gatgcctaca
     9961 gatgaatgcg gttttaactg tgtaattctt tcacgaatct catctggaca gcttccagct
    10021 ttggcaacaa tttgaatctc gtctttcata tcttttagca tcataaatga aatgttgcca
    10081 tggcctcgaa cgcttaagac ccaacccatt actgtgacat ctgtgtctgc catctgtggg
    10141 gagagctgat tagaataatg ggttctgcgc cagtcctcaa gatcggttcc tatcatgtgt
    10201 attttggcta tttctcactg tatttatttt gtgaaagacg aaattttact ggctgtctat
    10261 ttgttgttaa ctaggtttaa gtgactttct ggtctggagc cggctcttcc tttttgaata
    10321 acatcacatc aagacttgaa atgtcatcaa gatcaaactt gtttagggac tttcttttta
    10381 tctttctgac tagtgtgatg tcgcgtctaa agaagaggtt tacagtccag tctattgcga
    10441 ctttaagttt tttctcaaat gtgggcagca tggtaagata ataggtcctc caaacaaacc
    10501 atgccggaat gccagacagg tcataaccga atattttggc aatgccatta ccttttccga
    10561 ttactgccat catgcccttg ctttcaaaat taaatggttt ttgggagccg tttttagtga
    10621 tggcgcattt gaggttatgc gttacaattt tgctttcacg tagtgcatgc tgtgctgtgg
    10681 gtggatagag actacctgtt tttgtatcag taattgctgc acagtcacct aatgcaaaca
    10741 cattatcata agacggcatt ctgagatagc tgtcaactac aatcctcccg ccgttgtgct
    10801 cacattctag gctggttatg acagaatcta ttgtgacccc ggcagtccaa atcagtgtgg
    10861 cgcaggggat tgtctcacca tttcctaaaa atacaaaatc ttcgccggca tcaactgcct
    10921 ttgtgtttgg aattattcta attccagcct tttcaagtga ttcgtatgct ttttgtgcca
    10981 atccctctcc aagttctggt agtattcctg cttttgcaga aacgagaatc attttgacat
    11041 ttgcaggatc aatgttgtga tagtaacttt ttatagaatc tttgacaaaa tcgttgagct
    11101 cactaatcac ttcgacgcca gcaaacccag ctccgactac tgccactgtc agaatttttt
    11161 cctgaatggc ttgattttcc tcattatctg caatttccat catgttgata aggtgagttt
    11221 ttattgcaat tgcatcctgg atggttttta ttgtaaaaga atttttttct attctcttgt
    11281 ttccaaaaaa gtttgttgta ctgccaatag ctatgatcaa ataatcataa tctagaacct
    11341 ttacctttcc gtcaaatttt cgtgtaatgg caactagctt gtttttaaga tcaactgatg
    11401 acaccttggc ctgtatgaat tctgaatctt tggaaaagac ccgcagcggg attgaaatgc
    11461 tgcttggatg aagcattcct gacgctattt cctgaagcat tggtgtgaac aaaaagaaat
    11521 tgtcctcatt taccagtgtt atcttcagtt gcttgttttt tttaatgatc ttttgtagat
    11581 ggcgcagaac ggaaattcct gcgaatccac cgccgagaat gagaattttt gtttggttaa
    11641 cttgggttgc ttgcaatttg ggtaccacaa ttttttatta cgatgattgt cttttggagt
    11701 tgactattct ttcgttgatg gaaaaacaag acctttcgtg tttttgtaat tatcatgcca
    11761 aaaattctaa caatgtcttt tatttttaca atataaaatg agaaaattcc attagacgtt
    11821 ttacaaacat aatgcatgat gttttttgac tttggttatg ctaaagtgat agtttgtttg
    11881 cagaaaacga acgaattaag ggatttcatg cctgattgtc ctgtgcagac aactgaaaaa
    11941 ataattaccc aaaatatccc atacaccaat tatgagtgat aactcggatt tggttgatta
    12001 tataaaagag actaggctga tactcgctga cagcttagat gtcttactaa atctagagca
    12061 tgaggcactt tacgaaaaag acgaagaact cgtaccaatt aacaatgtag ttgagcttgt
    12121 agacatgagc cttgatgtgt ctcactctga aaaattaaaa caggccgaat ctgaactgct
    12181 aaaacggcag ccaatttagt gttgtttgta aagacttcgt agttgctggt cttctgcaac
    12241 gaattcgttt attcctgcca agacgccggc aattacatcg tctgacagat tcaagacagg
    12301 attgatggta tttccgtttt tatctttaca tgtgatctct agcattaaat cattttcagc
    12361 taggcctttt ttgcgagaga gtattgtagc tttttcaatt tttgttgcag ggatcgtgaa
    12421 ttgctctccg tctggagatt caaaaattat tctatcaaag cctgattttt gcttttgaag
    12481 aattattttg ccatctagga cctgagagtt ccagcctgga tacccgtcca catagtcgca
    12541 tttgtactcg cgtcgatcta gtggttttgc aacctctgtt ttgttcgact cggcgctatt
    12601 tctaagaaaa ctcgcttgtt ctttgatgta tttccagaga ttgctttcaa attttttggc
    12661 agtgtaaagc ctcgctactg tggcaacaat ccccatcact ggtattacaa acaatggtgc
    12721 tgagacgatt aggttgttgc cccactggcc tgtgccaata gtcacttcaa aattttctgg
    12781 agtcccctga attttgatat cagtacttcg tcttattcca gccgcagttc tgagcatacc
    12841 tagttttcgt gcctgaataa agaatgtgga aacgggcgtt gtctgatcct ttgaaaatgc
    12901 cacctcaaag ttattttctg ataaatacgc ttcaatccta gttgcaagtc tttcaatgtc
    12961 tgtgctataa ccatgaaatc ttttggtttt tgtcattttt gtagtcaaac aaatttgtat
    13021 tatttatgat taatctgtgt gcaaaatcgg aatgaacaaa caaactgata actcagttaa
    13081 gagttcactg aatatcacac agcaaagact gtaaagtaat gtgccttggg agaaagtaat
    13141 ctcccaagac ttggttggca cagcgaccat atggttcgct atttggggat tcttaatgga
    13201 atttagtgat gaaatactaa aaacagttac gtagaattta tccagtttat tattctatta
    13261 aatcgaccaa atagactgat taatgagttc ccttattatt aagacgtgga gatttctttt
    13321 gatgaaattt ctgatctgag gagttttatc aataacctaa actatgtgca agaaagtatt
    13381 gtagtagtag agggtaagcg cgactcagag gcacttaaga aacttggatt taccgagcag
    13441 gtcttagagt ttcacagctt tggcgggatc acaaaatttg ccgactctgt tgcgcaccac
    13501 aaaaacttga taattctttt tgattcggac agaaaaggta agtacttgac aagaagaata
    13561 attgaacagc ttgaacgcag ggtgcgaatt gatctatcac ataagaagcg actagttgag
    13621 atcaccaaag gcaaaattcg cgcaattgag gaactaatca gatacgaatc acccgtaaac
    13681 gactacataa tttgattatc tattaaccgt gcacagtttg tattattata taataataag
    13741 aactgtttgt gcaaaacaat aaccataatt tacttaaaaa aattttaagc atgttttttt
    13801 gtggtattat tcatagacgg acatgtaaca agtaaggcgt ccttagaaaa aatcaagggc
    13861 atagtaaatg gcgatgttga tgagtttggt gtctcaacaa ttgaattatt ctacaacaac
    13921 aaggcacaag agatgtactg cattttagat gcaccggatg agggctccgt agtaaaacac
    13981 catgcaaaaa ttgggctaac atgcaatttt ataactcaaa ttgaaaaaat tcatacaaaa
    14041 actgctgaca acactgcaag acttgttgca ataggtgagc ttgcagcacg gcttgcacat
    14101 gatttgagaa atccactatc cgttatcaaa aacactgtcg agatcatgga atcaaaacca
    14161 aaattccgta ttgaggatag ggtaatctac tataacagac tgcaccgggc agtggacaga
    14221 atatctcacc aaattgaaga tgttcttgat tttgtcaggc cactaacact aacttttgag
    14281 aatcatctga taaatgacat aatagcatct gccatagaaa aaattaccgt tcctgattca
    14341 gttaggatca ccctcccaaa caatttcgtt tacttggcgt gcgatttcac aaaactcgaa
    14401 gtcgtgttta caaatctgat aataaatgca attcaggcaa tgaacaatgt tggccaggtt
    14461 gacataaaga ttgttgatct tgacaagcac gtcgtaatac aaattgccga tactggatgt
    14521 ggcatcccac aaaacatcat gtcaaaaata tttgaaccgt tatttacgac aaagcagact
    14581 gggactggtc ttggactggc gagctgtaaa aaaatcatcg agcagcacga cggtgcaatt
    14641 gaaatatcca gtacggaggg aaaggaaaca atttttacaa taaagctgcc aaagcgaaac
    14701 atcgttccaa gtcaagaaaa acaactagaa aaaaatacac tagtttcata atctaaaaac
    14761 aaaaaagaag ggaagttagt tacttccgat gataaatacc acgcttgcag tagtgctaac
    14821 atcctgatca gatgagaaca ctggcgttct tattgcagat tcagccatgg cgtcaaacgc
    14881 tcccttgtag tatgggatgg gaggtggcgg atagccgaaa tctgataggc tgacatgctt
    14941 tactccgatt atttggtaat caagaggggc aagtgccttt tctgcctttg attttgcgtt
    15001 tatgattgct tctgacaaca agtcgtcgct tacttgcatt tgcctttgtg gggacagggt
    15061 gtagtaaaca ctatctactc tgtttgcacc agcactgact ccgctgtcaa ttatgtcgga
    15121 tgccaagctt aggttctttg tctcaacacg tattatgttt gagactgcat atccaacaag
    15181 tttttgcctg tatatcccag tttccttttc ttggtatgat tcatagactg ggtagatagt
    15241 cagttgtgca gtgtttatct cattgtctgt gatgcctgct gccctgattg cttcaactac
    15301 tgcagtcatt agctgcgagt tggcatcaag cgcttctttt gctgttgttt cttgcacttc
    15361 gacaccaaac tgtacaacta acaaatcagg gtcaactgat gtggtagctg tccccgtcac
    15421 agagattgtt ttctctcttg acggaaatgg tgttggctct tctgtttgag cttgggctac
    15481 gattcctgat ggaattgcgc caaggacaaa cgccagtgaa agaacaattc ctgttgctgc
    15541 tgcaatcgta attgttttgt tcatggcgtg tataggaaat ttttctttat tacctttgat
    15601 taaaatagga ttgaaccgta gggtttttta ggttaaatgt agccgtattt gtcttatctg
    15661 taaatgatta gagagaaatt tgaaagaatc agcgaatttt cttgtgccta tatctgttct
    15721 taatgtatct tgtataatat tgattgtgag ataatgcata tttcataaaa tcatcatgga
    15781 ttttttttga tatgccaaag tactgatagg ttccactctg ttgaaattct atttctagtg
    15841 tttgcgttga ctcatcatat ccaattattc tgatattgcc agagtctact cgtattcttt
    15901 ccatatcttc cttaaatcta gatgtatgtg ttttcttcct atgttttttg attttctata
    15961 atggatttct ttaactcatc aaagtcttct agatattcct ttttcattaa attctcaggt
    16021 tttgttttgt tgtttttgtt cttttggcgt atgggtgcgg tttttggctg tgtcatacta
    16081 ttagatacac tcagtagcat atatcttagt tacattaaaa cagtttattc aaggtttttt
    16141 gatttaaaat atttcaacaa aaaaggtgtg tttagattgt tgtcgagtat gccccaattc
    16201 caaggccaac gccaatgccc aagatgaaaa acaactcatt ccaagccatg acttttttga
    16261 atggtgctct aatttcagaa ttccagtgat ttgatgttgt ttttatgcca taacaacagt
    16321 gtgttgtgtc aataagaacc tagttagcca tcatgaagat tttgcttagg ctgccgtttc
    16381 aggtatgaaa ttaagaaagc gttatgttat tagtcagaag caccatctat aagcatgtca
    16441 tatgttagaa cgatgccgca tcgacaaatg ctagaaagat ggatgacaaa agtaggaatt
    16501 gagttatttg ttatcatcaa caatagtggt agaataatag actcgtttgg caacaaggat
    16561 ctcagcatat ccgatgaaaa acaacgcttg ttacttatgc aaatggcact ccaaagttct
    16621 atgcaaggtg actataacga agaatttggg aaagtaagat cttgtatagt tcaacgaaac
    16681 aagttaaaat ttatctgctg tccaatgggt gaagataaaa ccgctctagt tgtcgcaaat
    16741 aaaaccattc ctgacataga tatcatcaac cacattaatc aactagcaaa tgacacttac
    16801 atagttgctg agggagcaac acttctagta tgaacctcta tgatctgaag cagcttcatt
    16861 gccacaagtg tggaaaatgt ataggcgaga ttcattacga cgcagagatc accatgccgc
    16921 tttgtggtcc ttgtgccaat ccaatgcctg aaggcgatga caagttatta tatactgcaa
    16981 gtaaatacgg cagatctttg gtaaatacaa tttccgccta agactctcat aattatttaa
    17041 tcaaaaaaac tgactaaatt gatctataat ctacaaaata tgtgattaaa tttcttcaga
    17101 cataaaaaaa ttaacctaaa agctgttgat aaacgtagaa ttcactcgac aattttaata
    17161 agacgtatca aagcagccat gttttatggg cgattctgaa gatccaatgg agattaacga
    17221 caaagttgaa attctttcaa cagatgatga taagataaaa tcagttggtg agcttttgag
    17281 ctcagattct agtagggcaa ttctaaaatt attgttcaat gatcagctta ctgcaaatca
    17341 aatcgcacaa aaaaccgaga tttcattgcc cctagtcata tatcacctaa aaaaaatgca
    17401 agagactgga atggtcaaga tttcacaaac aggaaaaaac acaaagtcgc acgacatgaa
    17461 atattacaca gtagacaagt ttgcaatagt gattttgcct tcaggcgcaa cagaaaaggc
    17521 aaaatcaagc aaatcccttt tcaactcgtt taatcgaatc tacaggtttt ccatgattgg
    17581 cggcgcagca attgccgctt ggttttcatc acaattcttg caaatacaac aagcatcatt
    17641 gccaacagaa agcatgaggg gcgcagatgt gatgatggag caggctgaag aagccgcgga
    17701 aaaatcctta caggcaccag caccatccat tacagaatct gtgccatttg cagcgcaagg
    17761 agacatacag acaggcgagc cggcaaccga catttacatt tcaatcattg ttacgcttgg
    17821 agttgttgta ttggggctca taattgagag agtattgcgc gctcaaagaa aataacacta
    17881 aaaaccagac aacacattac aaaatcatgg ctttgacaaa cagagaaaag gcactagttg
    17941 tcatatcaaa cgcaatttca gtctactcca tttacacaaa aaatccagac atgctgccaa
    18001 agaacatatc tttgattgac tttgtcctaa agtctacgcc tgtggagcta aaaaaagaga
    18061 tttcaatgga cctaattgat gagattttcg attttgtctc taaaacacaa atggaacttt
    18121 cataaagcct caaaattaac agagatcaaa ataacatggc ttcgattgct acgcttggtt
    18181 cccactgcgc actgcaggtt ctaaagggag caaaagacga aggcttcaag acactccttg
    18241 tttgtgaaaa aaagcgcgag aatctttacc gcagattcaa attcatagat gaattggtat
    18301 tagttgattc attcttagaa ctacttgact caaaatgtgc gtcaatttta gaaaaaaaca
    18361 aggcagtctt gattccacat ggaacactta tcgcgcaaat gtcgacagaa caaatcgagt
    18421 caataaagac accaatcttt ggaaacaaat ggatcctcaa atgggaatca gacagattgc
    18481 taaaggaaaa actgatgctt gaggcaaatc tagacatccc agcatcaatt tcagacccaa
    18541 aacagatcaa aaaactcacc atagcaaaaa gacatggggc agccggtggg aaaggatatt
    18601 ttcttacgac aaacgaggca gattacataa aaaaacggga caggcttgtt tctatgggct
    18661 taatcaaagg cgatagcgac ttgtatttgc aggagtatgt catgggcgtt ttggcatatc
    18721 tgcagttttt ctattcacca ttaaaggacg aactggaatt ttttggagtg gataaaaggc
    18781 acgagtcaga cattgaagga ctggcaagaa ttccagcaca agaacagctc ggcacagatt
    18841 acatatcatc gtttaatgtt attggaaaca gcccgatggt tttacgagaa tcgttgttgc
    18901 aagatgtata tgatatgggg caaaaatttg tcgatgcctc aaaaaggctc gtatcacctg
    18961 gaatgaacgg gccgttctgt atagagggcg tttacgatca agacggcaag ttctggtcct
    19021 ttgagttttc tgcgcgaata gttgccggaa caaacatcta catggatggc tcaccatact
    19081 cgacactgct ctatgatgtg ccaatgagca tgggtagaag aatagccaga gaagtaaaaa
    19141 atgcggaaaa ggcaaaaaaa ctagataaaa tcactacttg aagagccttt gtcgcatcct
    19201 tgtgagaata attaccgttg ctgacacagc acccattatt gccaaggcac caaggggaaa
    19261 ttctggtatg acactagttc caattatctc aatttcttca gaatctgctg gaattggaaa
    19321 tgtaagtgcg ggattgtctc catcatatgt tattgtgtag tcagcctcaa gtccatcaac
    19381 tagcacaaca aactctgcgt tttgcgcgct cagcaattcc gaggaaaatg aaacctcaaa
    19441 gaacgagtct tcgacgtttt gtgttccaat aagcagtgat gtagattctt ggtcaatggt
    19501 cattgcaaga agttcaccat caaagctgta taaaacatcg aatgtgtttt catcaacttc
    19561 aagcttgtat gttccttgat cagcaagtga cagtggaatg atggaaagca ttacagttga
    19621 tacgataacg aatagataat ttttcatatg caaatgtcta tttaatctat gaatataggt
    19681 tttttcagtc attttaattt taaaattgtt aaataacaaa aattcattac aaaaataaaa
    19741 aatatgattg taaatcaatc atctaagatg attgattttg gtttcattaa ttcatcatat
    19801 gttggatttt ttggctcaaa tccggcgcat tggaatgtgt actgctcgag tactgcaaat
    19861 ccatcttttc cagtaaagct ttcggccttg tcaaattccg tgtttacctt gtagtttgca
    19921 acaccgcacc gaccataatc aaaactacga acaactttgc cttcttgcac cagatctaca
    19981 gttatgccaa actccttgta tgcatattca cctagtgagt tttttccacc atgtacgtac
    20041 gtttgatcca cagctctgtg tagaaacggt gtatcgccag caactcgttg gagtgtgaat
    20101 tctggtgcta ctgactttgt tgtgaaacta ccagatgtgc caaacaagcc atttagttgt
    20161 acaaacgtct ggaatggata tgtgacggtt gtttctctga acttgaatgt tactagtgga
    20221 tagacgcttt caccaaaaag atacgcgtcg tttccttgtg gcgattcagc tgctactatc
    20281 tcttctgtct gtgaaaagct tacaaccaat gcaattgttg aaattgcaat cgctgcggcc
    20341 atgactgtca tcatgattgt tgttttcatt ggtgtgcata atttgtttat tgataataaa
    20401 ccgtctaaag aattcagata cgtgttacaa cgcagtgttc taaattctta ttacatcatg
    20461 gcatgtgttc taaaatcata ttccacgatt gcagattctt gtctggaatt taaataatca
    20521 tgagaagtcg atggaagcgt gaacaaactt ttcgccgtta ttccaatcat gttagctgtt
    20581 ggaataggca taggatttgg aatattctca gtagcaaaca atgcagactt ggaatcatca
    20641 caaactcctt ctaaaaaaat tgtcactttg gcaatacagc caactgccca agcaagcgac
    20701 attgagtccc aagcaaaaga gctggaacag ttcttagaag aaagaacaaa ctatgacgtg
    20761 caaatttatg tcccaacctc atatgcgggg gtggtagagg ccctaaggtt tggaaaagct
    20821 gacatggcat ttatgagcgc gtggccatcg tatcttgctg ttcaaaaagc agatgccaca
    20881 ctagagattg ccgaagtcag agaggtagta ataggagata ccatgacagc tgaaacatat
    20941 tattactcgt actgggtggt accaaaagac tccccataca acacattaga agatctaaga
    21001 ggaaaacgtg cagcgtttcc aagtccgctt tctacatccg gttatgtaac accaatgaaa
    21061 accttggtgg aaacaggact gattactgta gaatctggca aagaaattga tccaaaagca
    21121 tacttttcag atgtcatttt tgctggaggc tatggccagg catgggaggc actaaagaac
    21181 aacaaagtcg atgtaagcat tatagctgga gatgtaagcg aaaaactcta tcgtgaggta
    21241 cgtgataaca ccaaagtagt tcacgagcaa ggaccaattc cgtcgcatgg tgtagtattc
    21301 agcaaagaca tggatgctga ggcaaaacaa aaagtcaaag atgcattact tgaaatgggc
    21361 aaagatgaga gctccaaaca aatcatgaaa aagctcgtgt ctgcaatatt tgtaggcttt
    21421 gaggagacca cagccgacca acatcttggg aatctgcaag atgcgctaca attaacaggt
    21481 ttgaaatata cagagaaagt ttagtcgtaa catcatgtcg gatgttctca gcatgaacca
    21541 aattcagctg attatcaatg aaagaaaaat ccttaataat atctcaatga gcgtaaagca
    21601 aaacagcgca gtaacaataa tggggcctaa cggtgctgga aaaactactc ttttgaaaat
    21661 tgccttgggt cttttgcaac ctacttctgg agaactatac ttgtttggaa aaaagggctt
    21721 ggataaacaa aggcgtaatc ttcttggcta cataccacaa aatttggggc ttgtcaacga
    21781 gctaagcgtt ttttcaaatg tgatgatggg tgcgttaaga cgcatgccac gctggcgtgc
    21841 gatcacaaac atgtatccaa aagaattgct aactgaggca tattcgttga tgtatgagct
    21901 aaagatcact cacctcaaag acatcaaaat aaaaaaactg agcggaggtg aaaaacaaag
    21961 agttgcaata gcaagaacac tcattcagaa accctctatg atcctagccg atgaaatgac
    22021 tgccagtctt gatttcaagg cagctgttgc agttatggat atttttgcag aaataagaaa
    22081 gaagaaggga atcacattac tgatgaccca tcataaccca gacattgcaa agatatacag
    22141 cgatactgtt ttcattatga ttaatggcgt gatagagaaa accatggaat caaaggagat
    22201 gagcgaagat acaatatcaa ggctttatga ggcctagtcg ttttcagtac acatggattg
    22261 tgttgcttct aataataatt ggaagctcta tacatttggg tttcaatcca ctctcgataa
    22321 ttgaggatgc agataattct ttaataataa tccaagaatt actggcattt gatttcagtg
    22381 tgggggacaa agttctcttc tccctcattg agacactgga aatggcttta attggatcaa
    22441 gtatgggatt tgtattatca attccagctg cattattggc agcaagaaac gtgtcaccgc
    22501 ctttcattag tgctatcttc agaggtattt tgggaatatt gtggtcaatg ccacctctgc
    22561 tttgggcaat actgctagtt gtgattgtgg gcctcgggcc aaccgctgga atatttgcca
    22621 tagcgcttta catcatgggt ctgagtggaa aatatctata cgaaatatac gaatcacaga
    22681 acctttctgc atatgatgcc atgcatgtaa ttggtgcaac acgaatgcaa attgccaaat
    22741 ttgtaacaat tccagaagca ctaccgcata tgagtaatca atatctgttt ttgctagctt
    22801 acagtgtgag agaatcatca attcttggcc ttgttggggc aggtggtatt gggttctaca
    22861 taatacacca tcttgaaagc ctcaattatg gaaaggccgc acccttcata cttgggatat
    22921 tagctgtaac gctaggcatg gattatatca gcacaaaatt acgcaaaagg ctcgtttatc
    22981 attaaaatca aatttaatca aaagttataa tcgctttgtg gttattgagt atgataaagc
    23041 aatgaagaaa atatttggtc tgctaatagt tgtattaatt acaacaggag tgcttgtgca
    23101 aataaaccca atatttgctc catcacatcc agtttttgca gggcaaaaca tgttggtatt
    23161 ttacggaaag ggtactactg atcagtcaag cccgatgcca gatgaagtaa tcagaaccgt
    23221 gatcaataag gacacggcta caattgtgca tcctgcaaaa ggcattgaaa tcattagaat
    23281 gaatattcaa acaagtgatg catgcattca aactcaagca actgtgtgtt ttgatgggat
    23341 agtcaccgat acaagaaacg tagatgtgca caaaataggc gacaaaatag gcataacatt
    23401 tgattttcaa aacaaaaaac aaatcatatc atttgattca ggattgatgc aaggagtgtc
    23461 attcacagtt gatctttcaa aaaccatcat acgtcttgat ggtccactca cagttacatt
    23521 ggcgcaagaa ggtgggtttg caggcgtgca aaacacattt gttgttgata tggcatcagc
    23581 gcagctaaca aaaaacgact cgataattat gcttgatgaa gattcaatca gtgaaatcac
    23641 aagcttaatc aaaaaaatca aattatttga tgtcgatgag gagacatatc ctccgataga
    23701 gggctctgct gattacttta catattttat tcagatatcg catggtcaaa ttcaaaaaac
    23761 agtttcatgg acagacacat cagaaattat tccagaaaag atgaacgcac taaaagatgt
    23821 aattataaaa atatctagca atatccaaac cagtgagccg gctccagata tcctacaagc
    23881 cgagttggca atagattttg taaagaatgc cccaacattt gcctttgatg gaatgggtga
    23941 tagcttaaca gtagaagacg tgatgatttt agaatcattt ccagtgcaat acgtcgtaac
    24001 aattaacttt gagtcactac atggcggata cggggacaga accggccagt acttaactca
    24061 ggcaataact ccacacacaa tcatagttac cgtagttgat gggcaaataa catcagcaat
    24121 cattgacgac atatgggacg agctaagtca agaaacaata gaataacgaa aactagctta
    24181 tttctattat tacggcggcc ttctcagagc tatccaactc ctgctgaacg gcgccaagta
    24241 tgtgtgatgc cgaagtcagg ctcgaaaaat cgataagctt gaggttgttt agtaggtaca
    24301 cctgcggtgc aatatcgctt cttcttgatt ccaaaaaatc atcaagctct tcaagaattg
    24361 agattttacc accttggaat aacttgccat ttttaattga aagcttttgc cttccaaacc
    24421 tgtccaagac taggatgtgg tgcttgtttt tcttgctgaa aaactttttt tcggaaacat
    24481 gtcctatgaa aacaaaataa tgatcagtct caaagctaag ctcgactagg tctttgttta
    24541 tttgcatcat ctcaccagca cgtattagtg cctcatctag tgtaaagaca ttttttgcat
    24601 tctcagagct gtcaagctcc acgttacctg ttgcagaaac tcgaagcagt gatttttctg
    24661 agataaactc gctgtttatg acaatagatt caggaactgc gcccctgtcg atcattagcg
    24721 catggatctt tttgtgcaga tccgaaatta tttctggtgt cggttttgta attgtgtgtt
    24781 cgatttcttc ttgaatcatt gaagacgcca caccaattga tgatatcact tcggcatgtt
    24841 ctgccttttt gtaaggtatt gtaagctgct ttgccacaaa cggcacaagt acagatgccc
    24901 ctcctccacc acctattatc tgggtttttg tcttgtccat tttgaattct tttagtatct
    24961 ttgatactgc ctttgtgatt tcaaaagatg ccgtttgaat tattgacatt gccacttcgg
    25021 gtccagatat tccaatctca cgacccagta gttccatcgc tattttcgca gagttttggt
    25081 ttgcatacga gtaatcgttt ttttctatca tgccaagcgc atttgcagca cacgtattcg
    25141 ttattgcaaa cgtctcgcct tcacaccgta ttgccgcata tgactcagtg tcattttctt
    25201 ttggtctgac taggactagt tttccttttt tgagcacctc tggctcggca aaacacgcat
    25261 aacgcatgcc tgcaatgtgt gcactcctcg ggccgacctt ccagacgcgt ttgtccctca
    25321 actttaccat gctgccacca gctacaccta aaattctaac atccattgag cggatgcagg
    25381 tagggtgatc cttaattgtt acatatcgta cctccggttt tccatttttt attatgcaga
    25441 tattggtgct agtgccgcca acttcgataa atattccgtt tgtaattttg agatacagta
    25501 gtgcacctgc aacacttgct gcagggcctg aaaggattgt aagaattggt ttggttctaa
    25561 aggtgtccat gctagtgacg cccccatcac ctttcattat cataaggggg gcagtcacac
    25621 cggcatttcg tatcgcatct tcgacaaaat ttgcaacctc aaacgttttt ggaagaacgc
    25681 ttgcgttgat tgcagcagta agtgttctga tttcaaggcc gtaaattcca gaaatctcgt
    25741 gtgatgcagt ggctggaatt tgatttgcca ttgcgttttt catcacaaac atctcatttg
    25801 atggatcgtc aactccaaag gcttcagtag ccactataac ctgtgcacct ttgtttttca
    25861 gatcagtcag tgcgcgcttc acttctgatt ctgaaatcag atgtgatgta tctagaaaaa
    25921 catgttctgt tttaagactc tgatcagaat taaacttggt atcatgcagg tttgttcttt
    25981 tgatcacttc gcctttttca ggcgttacac ccatagctat tatgccaacc tttgcagtat
    26041 ctgattcaag caatgcattg attgcctgag tagtgctgtg agcaataagc tcaatttcag
    26101 aaatttcaat ctttgattct tcaaggaggt ttgataatgc cgtaacgatt ccttttgaga
    26161 cgcctttttc tgctctgtgt gttgtcggaa cggtggattt tccaattatt tggccggttt
    26221 tgatgtctat tgctactgct tttgtgaatg tgccgccaac atctattcct actctaatgc
    26281 gtctcatcga tttcacttct tgatttacgc atggctgata tcttttcttc ccttttaggc
    26341 ctcaggatta gtacgtatga cgcagctact aggtatacgc caaggaggat tatttgtgag
    26401 actagagtct ctaatgttgg atagattcca gtcattgttg ccatgttaat gtctagtctt
    26461 ggaattattc caagcatact ggtatatggt acgatgtcca gcgtttgaag ttctcttatc
    26521 gcatttccca aaaatgcaat agatagatac gcgccaacac ccattgtcag gccaaatagc
    26581 atttttagtg ggagcctt
//
LOCUS       DASWKH010000008         7788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_388124, whole genome shotgun sequence.
ACCESSION   DASWKH010000008 DASWKH010000000
VERSION     DASWKH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388124"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..567
                     /locus_tag="VGA92_00815"
     CDS             190..567
                     /locus_tag="VGA92_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX9844986.1"
                     /translation="MKITYLELDTILEAIERHLGWTNTKATYLNKSNLQFILEATKTK
                     FNSEKKPLQKKSSYLLTQIVQSHPMADGNKRIAVILTETFVGMNGAEITASDNEIFEK
                     VTSFAAGKITESDIRKWLEENIR"
     gene            753..1979
                     /locus_tag="VGA92_00820"
     CDS             753..1979
                     /locus_tag="VGA92_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9844987.1"
                     /translation="MNGREKIQLSVIGVLLVMLAVGFTTNFESFVTRAVLSEQAPQIT
                     VIGEKQNDNAEPKDTVRPAMLATETQAKKQSSQLTLMFKQVENSVVQITSKVTSINPN
                     VIINGNPLESQSTKLGSGFVYDTSGHIITNNHVVEGAKTVDVRFVDGNIYSANVIGTD
                     PYNDIAVLQITDDFSEENLQPVILGDSSQLEVGQQVIAIGNPYGLSGTVTTGIVSQVG
                     RLLPNSDAGYSIPNVIQTDAAINPGNSGGPLLDMQGHVIGINTAIQSGTGEFSGIGFA
                     VPSNTVKRLIPSLIENGKYSHPWLGVSGTSLTPDIAKQIGLPKNYKGVFVTTVIPDGP
                     AQKAGLQESSYNINREIKGGDVIIAMDGYQVRDIDDLIIYLADNKNVGDTVVLQVNRN
                     NNLVELTAMLQERPAN"
     gene            2015..2158
                     /locus_tag="VGA92_00825"
     CDS             2015..2158
                     /locus_tag="VGA92_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844988.1"
                     /translation="MKKEVNSITGGFSNKFLDELAKEHDKAKDLANKQKELKQKHNQS
                     VLQ"
     gene            2172..3116
                     /locus_tag="VGA92_00830"
     CDS             2172..3116
                     /locus_tag="VGA92_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844989.1"
                     /translation="MGKSLTLVILAALVFVVFSTAEAATIKQTMEGGMDVTITYPDSV
                     IAGRDFAVSFLVKNNGWEDKQDIYLVITTQDDSIATKNGTLSVTRLSKEGSYGGNIDF
                     TSSAGAQPGTYYLNALYSQVLLSNNETPMPPTQKNIAIPVELKGQPEIQLNTVTPTAI
                     FPNAEFAFDVEIISKDIDLKDVSVELVAPSDITFRGQSMHTFSSLQKNTPLLIHSQIV
                     TESADIPSEHKLPFEVIVKYTDDTGEEKTTSKTVSLILRPRTFMEITPDGGIWVGSVF
                     LAPYVSIGTIVGIPAGALFSILIHRLVKKKDTKKKKAK"
     gene            3121..3441
                     /locus_tag="VGA92_00835"
     CDS             3121..3441
                     /locus_tag="VGA92_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9844990.1"
                     /translation="MKIEFDVKKYIDEIKNSDEYFHTFIDRANIAAGVLVLEPGEEDT
                     QAPHDSDELYYVIRGDGFLKINNKDYEISEGKAYYVQKDVPHKFFGNKKEIVALYFFS
                     GHDS"
     gene            complement(3422..4051)
                     /gene="purN"
                     /locus_tag="VGA92_00840"
     CDS             complement(3422..4051)
                     /gene="purN"
                     /locus_tag="VGA92_00840"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9844991.1"
                     /translation="MLNLGILISGRGSNMETILKSIKKQKIPIKPSVVISNNPKAKGL
                     KIAQRLGVRTEIIESKDFVGTRWEYDQKIIKTLSKYGVTEKNGLVCLAGFMRIISPEF
                     IRKYKNRILNIHPALLPSFAGLHAQKQALEYGSKYSGCTVHFVDDGVDTGPIVLQAIV
                     RIKDSDTEETLAKRILAKEHKIYPQAVRLFAEGKISISGRRTIIKNHGR"
     gene            complement(4082..4897)
                     /locus_tag="VGA92_00845"
     CDS             complement(4082..4897)
                     /locus_tag="VGA92_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9844992.1"
                     /translation="MHHIKESVDRILKNAQEDAARLLRNSQEYEEGPEPQEFSVPAGN
                     ESSILKKDKLHTIGEMSSRLAHDLQNPLSIIKNTLEIIKLKQPRIDEKTKENYERIER
                     AATRMSQQIKDVLDYVRTSNLLMEKASLTSLLKAVIADIDVPECTKIILPTEDIPVYG
                     DIKQIEVVFSNLILNAMQAIEEKGKIMIQASETDGMTVIDVIDNGQGIKKEDLSHIFE
                     PLFTTKQNGTGLGLASCKSIIDNHGGIIECSSIVGKGTVFTIKLPILRSLSKT"
     gene            5037..5321
                     /locus_tag="VGA92_00850"
     CDS             5037..5321
                     /locus_tag="VGA92_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844993.1"
                     /translation="MDVADIQIKKALVAFAIEKALLEMGEPVLQKVAKTLKDDYNCYI
                     PDCYEHPEYLKRILSDLYGNAHDAIISSIKNSLHDFSHQEPIQKFVSAIE"
     gene            5323..6120
                     /locus_tag="VGA92_00855"
     CDS             5323..6120
                     /locus_tag="VGA92_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844994.1"
                     /translation="MRTTLDAVLTKKYLVVALAACAAVILASNFISQDTAILVGNLTY
                     IPAAGSLLILSLLILARFGTTGHHGVAWFSFAAYAISLFIAEMLWIVQELYLQIDPFP
                     SAADIFYLTSYPFLLMFYVSYLQPVKNAITKKMIIASLVFSVGILIPGLYLALGPSTD
                     ADLFDVILGSIYPVFDAMVLIPALIGVSLFFKGQVNLMWTLFCLGTISTFVADSAFLF
                     AQSEDSYYTGNPMEIPYFWNYVLLSFGVGSHLVLFQKSKTDKLEDLR"
     gene            6254..6415
                     /locus_tag="VGA92_00860"
     CDS             6254..6415
                     /locus_tag="VGA92_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9844995.1"
                     /translation="MSETDDKIVKLRDPLFVIETSLAIIKNRSSDEKIQPELKRIESA
                     LTRIEQIIK"
     gene            complement(6418..6771)
                     /locus_tag="VGA92_00865"
     CDS             complement(6418..6771)
                     /locus_tag="VGA92_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9844996.1"
                     /translation="MTTAVVVDDDKDTVDVFCDFLDMKHVKVLGRGYSGKDALELYQI
                     YTPDVMFLDLMMPEYDGFYALEKIRKIDPAAKIVVITADLRADSAKKLDELKPTEIFI
                     KPYDLERITEMLERI"
     gene            complement(6771..7268)
                     /locus_tag="VGA92_00870"
     CDS             complement(6771..7268)
                     /locus_tag="VGA92_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9844997.1"
                     /translation="MAILILDDIKIRKLPIVERLKHCQQIIQHEQDESKRWDAVWLAG
                     EMAENRIEGDPVFDKVSELMAWVLENDDNNVVKHEACYQIAARNMRSKIPVLMQAALN
                     DKSGLTKHEALESLGLMRATEAIELIKDALEDPNEDVKQTASFVIKRLRRMQNAGMYK
                     PSQII"
     gene            7355..>7788
                     /locus_tag="VGA92_00875"
     CDS             7355..>7788
                     /locus_tag="VGA92_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9844998.1"
                     /translation="MVGLDRLLAKSLDSLIRENLGNVTIQKIENRLFEKYGLSVTQSI
                     EQFHKLDAVLREFFGAGADGIEQRALKSVCEVKLANGQGQILIENPVLTKIILESFGD
                     EDKKKILGAINSEPLIISQIITNCKIPQTSGYRKVNALIDAGL"
BASE COUNT         2338 a         1586 c         1558 g         2306 t
ORIGIN      
        1 tgtgacttga gctcttcgac tgcttttttt accctctctc ttactgcagc tgctacctga
       61 attccatcaa ttttcgttcc agtcaatacg agttttcctt taatgtgatt ctaaattaac
      121 ttaaccgaac tctgactaga atcaaagtca tcgcgccctt ttatgctcta ctagcatttt
      181 tgggcaagaa tgaaaataac ctatcttgag cttgatacaa ttcttgaagc aattgaacga
      241 catcttggct ggacaaacac aaaagccacg tatctgaaca aatcaaacct tcaattcatc
      301 ttggaagcta caaaaacaaa attcaattcg gagaaaaaac cattacaaaa aaaatcgtcg
      361 tatttgttga ctcaaatagt acagagccat cctatggcag atggcaacaa aagaattgct
      421 gttatattga cagagacttt tgtagggatg aatggtgcgg aaatcactgc atctgataat
      481 gaaatttttg agaaagtgac gtcatttgca gccggaaaga taacagaaag tgatattagg
      541 aaatggctag aagaaaatat tagatgaaac atttggaaag attaactcca caaagaaatg
      601 agaatcacga tgaaattctc aaattagttg atcaaaaaac aaagaaacgc cttgatctat
      661 acaaacgcct ctctaaatgc tgaatattga cgaattatgc gtaataatta taggtaatca
      721 ccgacaataa atcatacttt attggtggaa ctatgaacgg tcgagaaaaa attcaacttt
      781 cagtaattgg cgtgcttttg gtaatgcttg ctgttgggtt tacaacaaac tttgaaagtt
      841 ttgtcacaag ggcagttttg tcagaacaag ctccccaaat aacggtaatt ggtgagaaac
      901 aaaatgacaa tgcagaacct aaagacaccg ttcggccagc catgcttgcc acagagaccc
      961 aggcaaaaaa acaatcaagc caacttaccc tgatgttcaa acaagtggaa aactctgtag
     1021 tacagataac tagcaaagta actagcataa atccaaatgt gatcataaat ggaaaccccc
     1081 ttgaaagcca atcaaccaaa ctgggatctg gttttgtgta tgatacgtcg ggccatatta
     1141 ttaccaacaa ccatgtagtt gagggggcaa aaacagtgga tgtgagattc gttgacggaa
     1201 acatctattc ggcaaacgta ataggaacag acccatacaa cgacatcgca gtactgcaaa
     1261 ttacagatga tttttcagag gagaatcttc aacctgtgat cctaggagac tcatcacagc
     1321 ttgaggtagg ccagcaagta atagcaattg gcaacccata cggtctgagt ggaactgtga
     1381 ctactggcat tgtaagtcag gtggggcggc tcttgccaaa cagtgacgct ggttattcca
     1441 taccaaatgt aattcaaacc gatgctgcaa ttaatcctgg aaactctggg ggaccactgc
     1501 ttgacatgca ggggcatgta ataggaataa acacggcaat ccagtctgga acgggagaat
     1561 tttcaggaat tggatttgca gtcccatcaa acaccgtaaa aagactaatt ccatccctga
     1621 ttgaaaatgg aaaatattca cacccatggc ttggcgtttc cggaacgagt cttacaccag
     1681 atattgcaaa acaaatagga cttccaaaaa actacaaggg ggtctttgtc acaacggtga
     1741 taccggatgg acctgcccaa aaggcaggcc tgcaggaatc ttcctacaac attaaccgcg
     1801 agataaaggg cggagatgta ataattgcaa tggatggcta ccaagtacga gacattgatg
     1861 atcttatcat ttatcttgca gacaacaaaa atgtcggaga cactgtagtc ttgcaagtaa
     1921 accgaaacaa caacctagtt gagcttactg caatgctgca agagcgtcca gcaaattaaa
     1981 gaatttaaat tcaggattct ccatggtcgt ttgtatgaaa aaagaagtta attctattac
     2041 tggtggattt tcaaataaat tccttgatga gcttgccaaa gaacatgaca aagccaaaga
     2101 tctggcaaac aagcaaaaag aactaaaaca aaaacacaac cagtcggtgc tacaataaag
     2161 aaggcatgtg catgggaaaa tcgttaacgc tggtcatctt agctgcgttg gtatttgttg
     2221 tgtttagcac tgcagaggct gccacaataa agcagacaat ggagggtgga atggatgtta
     2281 caataaccta ccctgattct gtcatcgccg gaagggattt tgcagtgtct tttcttgtga
     2341 agaacaacgg atgggaggac aagcaagaca tctaccttgt aataacaact caggatgatt
     2401 ccattgcgac aaagaacgga acgctaagtg tgactcgact ctcaaaggag ggctcgtatg
     2461 ggggaaatat tgattttaca agctctgctg gggcccagcc tggcacgtac tatcttaacg
     2521 cactgtactc tcaggtgctg ctaagcaaca acgaaacgcc aatgccacca actcaaaaaa
     2581 acatcgcaat cccagttgag ctaaaggggc agccagaaat tcagctaaac acagttacac
     2641 ctactgcgat atttccaaat gccgagtttg cattcgatgt ggagataata tcaaaagaca
     2701 ttgatctaaa agacgtctca gtggaacttg ttgcgccaag cgacattacg tttaggggcc
     2761 agtcgatgca cactttttca tcactccaaa aaaacacgcc tcttttgatc cactcacaaa
     2821 tagtcacaga gtctgccgac atcccatctg agcacaagct gccatttgaa gtcatagtaa
     2881 agtacacaga cgacacagga gaagaaaaaa caacctcaaa gacggtgtct ttgattttga
     2941 ggccaagaac attcatggag ataacacctg atggcggcat ctgggttggc agcgtatttt
     3001 tggcaccata tgtgagcatt ggaacaatag ttgggatacc tgctggggcg ctgttttcca
     3061 ttctcattca caggctggta aagaaaaaag atacaaaaaa gaaaaaggca aagtgatttt
     3121 atgaaaatag agtttgacgt aaaaaaatac attgatgaga taaaaaactc agacgagtat
     3181 tttcacacct ttatcgaccg cgcaaacatc gcagcaggag tgctggtctt agagccagga
     3241 gaggaagaca cacaagcccc acatgattct gatgagctat actatgtgat tcgcggtgac
     3301 ggctttttga agataaacaa caaagactat gaaatatctg agggaaaggc ctactatgta
     3361 caaaaagacg tgccgcacaa attttttgga aacaaaaaag agattgttgc cttgtatttt
     3421 tttagcggcc atgattctta attatcgtcc ttcttccaga gattgatatc tttccttcag
     3481 caaacagtct gactgcctgt gggtagatct tgtgctcttt tgctaaaatc ctctttgcaa
     3541 gcgtttcttc ggtatctgag tcttttattc tgactattgc ctgcaaaact attggacctg
     3601 tatcaacacc atcgtcgaca aagtgcaccg tgcagccgga atatttggag ccatattcta
     3661 gtgcctgttt ttgtgcatgc aagccggcaa atgatggcaa aagggcggga tggatgttta
     3721 gtattctgtt tttgtatttt cggataaact cagggctgat aattctcata aagcctgcaa
     3781 ggcaaacaag cccgtttttt tcagtcacgc catattttga gagtgtcttg atgatttttt
     3841 ggtcgtactc ccatctggtg ccgacaaaat ccttgctctc aattatctct gttcttacgc
     3901 caagcctttg agctattttg aggcccttgg cttttgggtt atttgatatg acaactgagg
     3961 gctttattgg aattttttgt tttttgatgg attttagtat tgtctccata ttgcttccgc
     4021 gtcctgaaat gagaattcct aaattaagca agactggctt ttctcaaaga atcaaacaat
     4081 attatgtttt agaaagggaa cgcaatattg gcagttttat tgtaaagacg gtccccttac
     4141 caacgatgct tgagcactcg atgatcccgc catgattatc tataattgac ttgcagcttg
     4201 caagcccgag gccggtgccg ttttgttttg tggtaaacaa gggctcaaag atgtgtgaga
     4261 ggtcttcttt tttgataccc tggccattat ctatgacgtc tattactgtc atgccatcgg
     4321 tttctgaagc ctggatcatt atcttgcctt tttcttcaat tgcttgcatt gcgtttaata
     4381 tcaaattcga aaagacaact tctatctgtt ttatgtcgcc ataaactgga atgtcttctg
     4441 ttggcaaaat tatctttgtg cattctggaa catcaatgtc ggcaatcact gcctttaaca
     4501 agcttgtaag tgaggctttt tccataagca agttacttgt tctgacataa tctaggacat
     4561 ctttgatttg ctgggacatt cgcgtagcgg ctcgctcgat tcgctcgtag ttctcctttg
     4621 tcttttcgtc tatacgcggt tgttttaatt tgataatctc aagtgtgttt ttaattattg
     4681 ataatggatt ttgcaaatca tgtgccagcc ttgaagacat ttctccaatt gtgtgaagct
     4741 tgtctttttt taatatgcta ctttcgtttc ctgcaggtac tgaaaattct tgaggttctg
     4801 gcccctcctc atattcctgc gaatttctca aaagcctagc ggcatcctct tgtgcattct
     4861 ttaaaatgcg atcaacactt tcttttatgt gatgcatact aactcgttat gaatttctta
     4921 agaatatttg agttagtctg tttcttgcaa acaatatccg cacgatcttg caataattgg
     4981 cgtcacggct caaaaagaca tgtggtttta taactgggga tcggtatttc aaaaccatgg
     5041 atgtagcaga cattcagata aaaaaagcac tggttgcttt tgctatagaa aaagcacttt
     5101 tggaaatggg tgaaccagtt ttacaaaagg ttgcaaagac actaaaagac gattacaact
     5161 gttacattcc tgactgctat gaacatccag agtatttgaa aagaattcta tctgaccttt
     5221 atggcaatgc tcacgatgca atcatatcat caataaagaa cagccttcac gatttttccc
     5281 accaagaacc aattcaaaaa tttgtttctg caatagagta gcatgcgaac tacacttgat
     5341 gccgttttaa caaaaaagta tctggttgta gcgcttgctg cgtgcgctgc agttattctg
     5401 gcctcaaatt ttatcagtca agatactgca attcttgtag gcaatctgac atacatccca
     5461 gctgcaggct cgcttctgat tctttcattg ctaattcttg caaggtttgg cactactggt
     5521 caccatggtg ttgcgtggtt ttcttttgca gcttatgcaa tttctttgtt tatagcagaa
     5581 atgctttgga tagtccaaga actatatcta caaattgatc cgtttccatc cgcggcagat
     5641 attttctatc ttactagcta cccattcctt ctgatgtttt acgtttcata tcttcaacca
     5701 gtcaaaaatg caataacaaa gaaaatgatt attgcgtcgc ttgttttttc tgtaggaatt
     5761 ttaatcccgg gcctgtatct ggcacttgga cctagtaccg atgctgatct gtttgatgta
     5821 attttgggta gcatttatcc ggtttttgac gcaatggtgc tgattccagc actaatcggc
     5881 gtctcgcttt ttttcaaagg ccaggtcaat cttatgtgga ctttgttctg ccttggaaca
     5941 atttcgactt ttgtagcaga ctcggcattt ttgtttgcgc aaagcgagga ttcctattat
     6001 actgggaatc caatggaaat accgtacttt tggaactatg tcctgctttc ctttggtgtg
     6061 ggcagtcatc tagtattgtt tcaaaaatct aaaacagaca aactagagga tctaagatga
     6121 ggttctcatc tggcttttct atgaattcta gcctcatctg ggccaaaact agccaatgag
     6181 atcttcgtat ggtgagaata tatttccaat tttggcagag ctatttttta taattacatg
     6241 acaagtaata tcattgtccg aaacagacga caagatagtg aaacttcggg atccgttatt
     6301 tgttatagag acctcgttgg caataataaa gaaccgttct tctgatgaga aaatacagcc
     6361 agaactcaaa agaatcgagt ctgccctcac aagaatagag caaattataa aataagacta
     6421 gattctctct agcatctcag taattctttc aagatcgtat ggtttgataa aaatctcagt
     6481 tggcttgagc tcatcaagct tttttgcgct gtcggctctt aggtcggcag ttattaccac
     6541 aattttggct gcaggatcga tttttcttat tttttctaga gcgtaaaatc catcatactc
     6601 aggcatcata aggtccaaaa acatgacatc tggtgtgtaa atctgataca attctagggc
     6661 gtcttttcca ctatatcctc ttccaagcac cttgacatgt ttcatgtcta aaaaatcaca
     6721 aaatacatct actgtgtctt tatcatcatc tactacgact gcagttgtca ttagattatc
     6781 tgggatggtt tgtacatccc cgcattttgc atgcgcctga gccttttaat tacaaacgat
     6841 gccgtttgct ttacatcctc atttggatcc tcaagtgcat cctttatcag ctcaattgcc
     6901 tcagttgccc tcattaaccc aagtgattct agggcctcgt gttttgtcag tccgcttttg
     6961 tcgtttaggg cagcttgcat caaaactggt atctttgagc gcatgtttct tgcagcaatc
     7021 tgatagcacg cctcatgctt taccacattg ttgtcatcgt tttctagcac ccatgccata
     7081 agctcagaca ccttgtcaaa aactgggtct ccctctatgc gattttcagc catctcacct
     7141 gcaagccaaa ccgcatccca tcgtttcgac tcatcttgtt catgttgtat gatttgttgg
     7201 caatgcttta gcctctcaac tatgggcagt tttctaatct taatgtcatc tagaataaga
     7261 atagccaata ccaactttgg aagtgacaaa attctattaa actgttgtgt tcacttgtgg
     7321 aaataataat ataatatgat ggtaatcaca tactgtggta ggcctcgaca ggcttttggc
     7381 aaaatccctg gactcgttaa ttcgtgaaaa tctgggcaat gtaacaatac aaaaaataga
     7441 aaacaggctt tttgaaaagt acgggctttc agtaacccaa tcaattgagc agttccacaa
     7501 actcgacgca gtcttgcggg agttttttgg tgcgggggca gacggcatag aacaaagagc
     7561 tctcaagtca gtttgcgaag taaaactagc aaatgggcaa ggccagatct taattgaaaa
     7621 tccagtatta acaaagatta ttttggaatc ctttggcgac gaagacaaaa agaaaattct
     7681 tggcgcaata aacagtgaac cattaattat ttcgcagata ataacaaact gcaagattcc
     7741 acaaacctct ggttatagaa aggtaaatgc actaattgat gctggcct
//
LOCUS       DASWKH010000009         6753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_760989, whole genome shotgun sequence.
ACCESSION   DASWKH010000009 DASWKH010000000
VERSION     DASWKH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760989"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGA92_00880"
     CDS             <1..384
                     /locus_tag="VGA92_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sialidase"
                     /protein_id="HEX9844999.1"
                     /translation="TKVEVPTSDWNLQKAKVTGYTQPSNKYFATRERLAYHPRDKYFA
                     GYGIKGGATQLTTTAPPPKPQIAPPQTAQSSSGGKTKKEQLEDYEKQYLARLQQQQQE
                     ARSRPPPPPTPTVSKGSMPKGWTPS"
     gene            complement(406..864)
                     /gene="bcp"
                     /locus_tag="VGA92_00885"
     CDS             complement(406..864)
                     /gene="bcp"
                     /locus_tag="VGA92_00885"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX9845000.1"
                     /translation="MIAEGEQVPKFELQDANGKMIKSSDFKGKKHVIYFYPRDFTPGC
                     TIEADEFSKEYKKFQKSGVEIIGISTDDVESHKKFCDKMKIPYVLLSDTDSQVCKKFG
                     VWGKKQFMGKEYMGIQRSTFLVNEKGIIFKAYPSVKPKGHAEEVLLALKN"
     gene            963..1667
                     /locus_tag="VGA92_00890"
     CDS             963..1667
                     /locus_tag="VGA92_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEX9845001.1"
                     /translation="MVQGRSWFAESIATFCLVFFGPLSVIMAAAAFGTGLSIEGILLI
                     SFAHGSAIGLMVYAFGHVSGAHINPAVTISMLVTRKIGIIDGIGYIVFQIIGAIIAAF
                     SLKAILPELGAKVNFGTQGGPSDLLNYSVMSGFAVELVLTFFLVTVIFMTAVHKKAPG
                     GIAGLAIGGMIFLIHLVGVPLTGASVNPARTLGPAIASGYWDFHWIYWAGPIIGAIIA
                     GLIMNYIFVKKAEESS"
     gene            1718..1792
                     /locus_tag="VGA92_00895"
     tRNA            1718..1792
                     /locus_tag="VGA92_00895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1753..1755,aa:Arg,seq:gcg)
     gene            complement(1795..3093)
                     /locus_tag="VGA92_00900"
     CDS             complement(1795..3093)
                     /locus_tag="VGA92_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845002.1"
                     /translation="MKTVNTTKHHVLTDEDCKAVQSDMGKKLTYEQSQTTTAKKHKIT
                     KKDQLLSNQDIRRWYDNLARSSIVTAEVRLRKLGKFCENNKITPKELIELGLKDQRAV
                     ADLLEDNITTMEKQRKAPQYIKAVITSVKSWLHHFDIEVKRKIRITNVDATPTLADER
                     VPDASELTELFNRAKLRAGAIMALMGKAGLRPEVLGNHDATDGLIIQDLPELEIVHGI
                     ARFAKTPPKIVVRRTLSKAGHKYFTFITESGAEKILAYLNQRMIDGETLSPESPVIAP
                     FSKYKRFRGKNEGRKFLSTPIVERDVRITMRPRFKWRPYVLRAFFDTQLLIAESRGKI
                     AHDFRVFFMGHTGSIEAKYTTNKSILPKVLTDEMYESFRKCQEFLDYETNTRQDESIT
                     NQRVVTEEEFERLVTEGWQYLGTTPNGKIVVKNDGARTNR"
     gene            complement(3090..3332)
                     /locus_tag="VGA92_00905"
     CDS             complement(3090..3332)
                     /locus_tag="VGA92_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845003.1"
                     /translation="MTKLNRSECSHDLESRKVDLGERKVSKMNFSHIVTLPKRFVNSP
                     FGEIRLVKMTLLEDGSLKIIPVRQNDEPDVFSTFKP"
     gene            3397..3966
                     /locus_tag="VGA92_00910"
     CDS             3397..3966
                     /locus_tag="VGA92_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845004.1"
                     /translation="MVGRRAACHVVGLQIYTYLPIYIVSYQPIIAEEARRTFHDGTRL
                     FRLVDTRMSHGIKVLKEDFQKCKENLAKLGYEKIQARQLSTSFPKYCPKCDKSDGYPH
                     LRLVKKTFSEKDKNFRALKKAKYQVYYNHSKPKYHQCFIGYWTIDNRIRLAKGIDIRK
                     MSPFYSLKEGQSVSFPLPTEHMKKPEVLR"
     gene            4021..4296
                     /locus_tag="VGA92_00915"
     CDS             4021..4296
                     /locus_tag="VGA92_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845005.1"
                     /translation="MSKTVSARIPKEMHGKLIEMCNKIGCTVNEYLTGSLELALDGTT
                     EKELGLDMDDESEPETKIDLKKQENKPIPEAKITKISYDNGKTWINI"
     gene            4914..6194
                     /locus_tag="VGA92_00920"
     CDS             4914..6194
                     /locus_tag="VGA92_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845006.1"
                     /translation="MLTIPDYTKYIEIVKNNVDQRWLHRETEKILTYYNTKKNTRILG
                     AIDDSANPLALLIYQAEKQAQNNQSSQIKVVFNELATIVRLGLYIENLQTKNIKGFAE
                     KLTELTTANKDQFEKVLFELDMASAFSKANHKISFIETNSKEQKPTPDLLVDENIEVE
                     CKKKDRLTKRDIKNNEHWQFLERKLLELMREQKKFYYVNLFFEEDPTGKSIYEILRKI
                     RKLIINNESGEFSLDVAKLKMYKICDFAEDFLLPIHAKSQHELQAQLTVDILDNVMKE
                     RIPNPDLLDYVKQKPDFDVTLPRVTLHPNGEVVLGEIMKFMFKMKQVSDRLKSVINSI
                     KDAKQQLTGNRCSIICVNFTYLAEKMIEHDFARLGEMINDVLGNNSSISAVAVTTESH
                     IRDANGLRFVHQASVIRNKSAKFPLPDDFIIRND"
     gene            complement(6210..6446)
                     /locus_tag="VGA92_00925"
     CDS             complement(6210..6446)
                     /locus_tag="VGA92_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845007.1"
                     /translation="MGLYNMGLTESIKTITELLAELNKTESNSELKRVASALRKIQRE
                     MEKQQRNAKRDLRISIAITSVIAFVIGYALKYYF"
     gene            6500..>6753
                     /locus_tag="VGA92_00930"
     CDS             6500..>6753
                     /locus_tag="VGA92_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845008.1"
                     /translation="MVESNEGKKEPEVRSESKSLKIKKIFNYIKSEKSHLINTIAILI
                     FFSLGIMSAIFASDIIFELSQQEWSDTKISQESSSKLLNLA"
BASE COUNT         2036 a         1319 c         1322 g         2076 t
ORIGIN      
        1 accaaagttg aggttccaac atctgactgg aacctgcaaa aggcaaaggt gacaggctac
       61 actcaacctt caaacaaata ctttgcaacc agagaaagat tggcatacca tccaagagac
      121 aagtactttg caggatatgg aatcaaaggg ggcgctacac agttaacaac aactgcacca
      181 ccaccaaaac cgcaaatagc accaccacag acggcacaat catcatctgg cggcaagacc
      241 aaaaaagaac aactcgaaga ctatgaaaaa caatacttgg caagactgca acaacaacag
      301 caagaagcta ggtcacgacc tcctccacca ccgacaccaa cagtatctaa gggttcgatg
      361 ccaaaaggct ggacgccgtc ctaaagaaat ctcttttttt attttctaat tttttaacgc
      421 tagtaatact tcttctgcgt ggccctttgg ttttacgctc ggatatgcct tgaagatgat
      481 gcctttctca tttaccaaaa acgtgctcct ctgtatgccc atgtactctt tgcccatgaa
      541 ctgttttttt ccccagacgc caaacttttt gcagacctga gagtcagtat cggataacaa
      601 aacgtatgga atcttcattt tgtcgcagaa ctttttgtgg gattctacat catcggtact
      661 aatgccgata atttctactc cacttttttg gaattttttg tattcttttg agaactcgtc
      721 tgcctctatt gtgcagcctg gcgtaaagtc ccttgggtaa aaatagatca cgtgtttttt
      781 gcccttaaag tcggatgatt ttatcatctt tccatttgcg tcctgtagct caaactttgg
      841 gacctgctct ccttctgcta tcatggattg catctgtatg tcctcaatta attatttttg
      901 tactcgatag atagtaaatt gactcgaata ttttatattg aaagtggagt ggctgacctt
      961 tgttggtaca agggagatca tggtttgcag aatcaatagc tacgttttgc ctagtgttct
     1021 ttggtccact atctgtaatc atggcagcag ctgcctttgg gacaggcctc tcaattgagg
     1081 gaattttgct aatttcgttt gcgcacggct cggcaattgg actgatggtt tatgcgtttg
     1141 gccatgtatc tggcgctcac atcaaccctg cagtcacaat ctcgatgtta gttacaagaa
     1201 aaattgggat aattgacgga attgggtata tagtatttca gataattggt gcaataattg
     1261 cggcattctc actcaaggca atactgcctg agcttggagc taaggtaaac tttggaacgc
     1321 agggcggccc aagtgatctc ttaaactaca gcgtaatgtc cggctttgct gtagaattgg
     1381 ttttgacatt cttcctagtc acggtaattt tcatgactgc cgttcacaaa aaggccccag
     1441 gaggaatagc tggccttgca ataggtggca tgatattttt gatacaccta gtcggcgtgc
     1501 cattgactgg tgcctctgtg aatcctgcaa gaactctggg tcctgcaatc gcatcaggct
     1561 actgggattt ccactggatc tactgggcag gcccaataat cggggcaata attgctggcc
     1621 tgattatgaa ctatatcttt gtcaaaaaag cagaagaatc tagttagagt tcatttttct
     1681 gcaaacgttt tttacaaatc tgtcttttgc tagtttaggg cccgttgcac agcttggata
     1741 gtgcgaacgc ttgcggagcg ttaggtcgtg ggttcgagtc cccccgggcc cgctttatct
     1801 gttagtccga gccccgtcat tttttacgac aattttgccg tttggcgtgg ttccaagata
     1861 ctgccagcct tccgttacca gtctttcaaa ctcttcttcc gtgacaactc gctggtttgt
     1921 tattgattcg tcctgtcttg tgttggtctc atagtccaaa aactcctgac actttcgaaa
     1981 tgactcgtac atttcgtctg tcagaacctt tggaagtatg ctcttgtttg tggtgtactt
     2041 tgcctcaatg gaaccagtgt gtcccataaa gaagacgcga aagtcgtgcg ctatttttcc
     2101 tctggactct gcaattagca gctgtgtgtc aaagaacgct cgcaatacat acggcctcca
     2161 cttgaagcgc ggcctcatgg tgattcgaac atccctttca actattggag tcgaaaggaa
     2221 ttttcttccc tcgtttttgc cgcggaacct tttgtacttt gagaaaggcg ctatcacagg
     2281 gctttcagga ctgagagtct caccgtcaat cattctctga ttgaggtatg ctagtatctt
     2341 ttctgctcca ctttctgtta tgaaagtaaa gtacttgtgt cctgcctttg agagtgtcct
     2401 tcttaccacg atttttgggg gcgtttttgc aaaccttgcg attccatgta ctatctcaag
     2461 ttctggcaga tcttgtatga ttagtccatc tgtcgcatcg tgattcccaa gcacttctgg
     2521 gcgcagtccg gcctttccca ttagtgccat tatcgcgcca gccctcagtt ttgccctatt
     2581 gaacaattct gtcagttctg atgcgtctgg aacccgctcg tctgcaagtg ttggtgtggc
     2641 atccacgttt gtgatccgta tttttcgctt tacttcgatg tcaaagtggt gtagccatga
     2701 cttgactgaa gtgattactg ccttgatgta ttgaggcgct tttctttgct tttccattgt
     2761 ggtgatgttg tcttcaagaa ggtctgctac tgctctttgg tctttgagcc ctaactcgat
     2821 cagctctttt ggggtaatct tgttgttctc acagaacttg ccaagctttc gcagtcttac
     2881 ttcagctgtg acaatactgc ttcttgctag gttgtcgtac cagcgtctta tgtcttggtt
     2941 tgaaagaagt tgatcttttt ttgtgatttt gtgttttttt gcagtggttg tttggctttg
     3001 ttcatacgtc aatttttttc ccatatcaga ctgcaccgct ttgcagtcct catctgtgag
     3061 aacatgatgt tttgtggtat ttaccgtttt catggtttga aggttgaaaa tacgtcaggt
     3121 tcatcatttt ggcgcactgg tatgatcttt agcgatccat cctctagtaa tgtcattttt
     3181 actaatctta tctcgccaaa cggcgagttc acaaaacgct ttggaagcgt tacaatatgg
     3241 ctaaaattca tttttgagac ctttctttct cccagatcca cctttcttga ttctagatcg
     3301 tgggaacatt ctgatctatt gagtttggtc atgatattac aataaccgcg gtttttttaa
     3361 tgacggagtc atgaaatatg cctaggcgtt tgcaaaatgg taggaagaag ggcggcttgt
     3421 catgtagttg gattgcagat ttacacatac cttccaattt acattgtatc ttaccagcca
     3481 atcattgctg aagaggcaag aaggacattt catgatggaa ccagattatt tcgcttggtt
     3541 gacactagga tgagtcatgg aatcaaggtt ctcaaagaag actttcaaaa atgcaaagaa
     3601 aatcttgcta aactcgggta tgagaaaatt caagcccgtc agttaagtac atcatttcca
     3661 aaatactgtc ccaaatgtga caaatcagat ggttatccgc acctgagact ggtcaagaag
     3721 acatttagtg aaaaagacaa aaactttcgt gcgttaaaga aagcaaaata tcaagtctat
     3781 tacaatcata gtaaaccaaa atatcatcag tgtttcatcg gttattggac aattgacaat
     3841 cgaattcgac tggctaaagg aatagacatt aggaaaatga gtccgttcta ttctctaaaa
     3901 gaaggtcaat ctgttagctt tcctcttcca acagaacata tgaaaaaacc agaagttttg
     3961 cgttgatcat aatccgtgtt tattagtcac tgtttaccgt ttgtttactg gtgtaaacag
     4021 ttgtcaaaga cagtctcggc tcgcatacca aaagaaatgc atggcaaact aattgaaatg
     4081 tgtaataaaa taggatgtac tgtaaacgag tacctgactg gatccttaga gcttgcattg
     4141 gatggaacca cagaaaaaga gcttggccta gatatggatg atgagtcaga accagaaacc
     4201 aaaattgatc tgaaaaaaca agaaaacaaa ccaattcccg aggctaaaat aacaaaaatc
     4261 agttatgata atgggaaaac ttggattaat atctaatttc ctaaaacctt aaagtttatt
     4321 caattaataa aaccaatatg ggaatctttg atattcttcg aagaaaaaaa aattcagaaa
     4381 atatagttag aataacacgt gttgacccaa caacaggaaa aacgatatgg tctgtgccag
     4441 ttacaagtca cacatctcaa agacacccaa aacctcaaaa gccatttaga attagtaaat
     4501 gcaaagaatg tggtcgttca gtaagacata cgtgtatctc agaacgtcgt aaaacaattg
     4561 atgtgggtgg gatggaatcg attagtcaga tttgggcaat ttcaaatcac aaatatggaa
     4621 atggaaaatg taggggttca aatcgcagat acaaaaaaca atttattaaa taaaatgctc
     4681 tccaacgaat cctaactttc gtcatgttct agttattgac gatttatctt tgttttcact
     4741 cttcatcaat atagtacaac tgacagcact tctttgtgtc tgacttgtgt ctgtgatttc
     4801 ctctatggga atatgatttt gacacagaaa tgacgtgaat catgctacct cattgctact
     4861 tcacatagaa ccaagcttta aacatttgca acactgttga tttagtcgtc agtatgctta
     4921 cgattcctga ttatacgaaa tacatagaga tagtaaaaaa taacgttgac caaagatggc
     4981 tacatcgtga aactgaaaaa atccttacct attacaatac gaagaaaaat actcgtattc
     5041 taggcgcaat tgatgactcg gcaaatcctt tggctctgtt aatctatcaa gccgagaaac
     5101 aggctcaaaa caaccaatcc agtcagatta aagtcgtatt taatgagtta gcaacaatag
     5161 taagattggg tctgtacata gaaaatctgc aaactaaaaa catcaaaggt tttgcagaaa
     5221 aactcaccga gctaaccact gccaataaag atcagtttga aaaggttctt tttgaacttg
     5281 atatggcaag tgcattttcc aaagcaaatc acaaaatttc cttcatagag accaattcta
     5341 aagaacagaa accaactcca gatttgcttg ttgatgaaaa catagaggtt gaatgcaaaa
     5401 agaaggatcg actgactaag agagacataa aaaataatga acattggcag tttttggaga
     5461 gaaaactttt agaattaatg agggagcaaa agaaatttta ttatgtaaat ttgttttttg
     5521 aagaagatcc gactggaaaa tcgatctatg aaatacttcg aaaaataagg aaattaatta
     5581 tcaataacga gagtggagaa ttttctctag atgttgccaa actaaagatg tacaagatct
     5641 gtgattttgc cgaggatttt ctgttaccaa tacatgcaaa aagccaacat gaattgcagg
     5701 cacaactaac agtagacata cttgataacg tgatgaagga aagaataccg aatccagatt
     5761 tgttagatta tgtaaaacaa aaaccagatt ttgatgtaac gttgcctaga gtcactcttc
     5821 atccaaacgg cgaagttgta ttaggtgaga tcatgaaatt tatgttcaaa atgaagcaag
     5881 tgtcggatag gttgaaaagt gtaataaatt ccatcaagga tgccaagcaa caactaactg
     5941 gaaaccgatg tagtattatt tgtgtgaatt tcacttacct tgctgaaaaa atgatagaac
     6001 atgattttgc aagactaggt gaaatgataa atgatgtttt gggtaacaac tcgagtattt
     6061 cggcagtagc tgttactact gaatctcaca ttagggatgc aaacggactt agatttgtac
     6121 atcaagctag cgtgattcgt aacaaaagtg caaaatttcc acttcctgat gattttataa
     6181 tacgtaatga ttgaacaacg aatcaatcac taaaaataat attttaaagc atatcctatc
     6241 acaaacgcaa ttacagacgt tatggcaatg ctaattctta aatctctttt tgcatttctt
     6301 tgctgttttt ccatttccct ctgaatctta cgtaacgcag atgcgactct tttcaattca
     6361 gaattgcttt ctgttttatt aagttcagca agtagttcag taattgtttt aatgctttca
     6421 gtaagaccca tattgtatag tcccaatttt gttttaaatt tgtaatgcct tattaggtta
     6481 aataattaag aaaatttgat tggtagaaag caacgaaggt aagaaagagc cagaggttag
     6541 atcggaatct aaatctctta aaataaaaaa aatttttaac tacataaaaa gtgaaaaatc
     6601 tcacctaatc aacacaattg cgatactcat attcttctca ttaggaatta tgtcagctat
     6661 ttttgcatca gacataatat tcgaattgag tcaacaagaa tggtcagata caaaaatctc
     6721 acaggaatca agcagtaaat tactaaatct tgc
//
LOCUS       DASWKH010000010         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1493609, whole genome shotgun sequence.
ACCESSION   DASWKH010000010 DASWKH010000000
VERSION     DASWKH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493609"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGA92_00935"
     CDS             complement(<1..239)
                     /locus_tag="VGA92_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845009.1"
                     /translation="MEKIKSPKYVQLASHMEFSRLVCALERAPRVSFLHEHDGKKILA
                     IQMDLLKERPVIYYVPIEHNGHYISYGFRNGKEEAG"
     gene            complement(297..1295)
                     /locus_tag="VGA92_00940"
     CDS             complement(297..1295)
                     /locus_tag="VGA92_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX9845010.1"
                     /translation="MPSMVVVGGFFGDEGKGKIISYLAQKDDPTIVVRGGAGPNAGHT
                     IEDGNKKYKVRMLPSGFLNKKAKLMIGPGVVVNPDVLHKEIEEFGVAKRTFVDSSCGI
                     IEKSHTDADSAGRLKEKIGSTGSGTGPANADRAMRTLKLAKDIDSLKPYLTDVAEQIN
                     LALSNDETVLVEGTQGTFLSLWHGTYPFVTSKDVTASGICADIGLGPKNVDDVMVVFK
                     SYVTRVGTGHLENELPAEEVAARGWSEVGTVTGRQRRAAEFNFNMAKRAIMLNSATQI
                     AITKLDVMFPECAHKNSFEDLSDRAKSFITKIEDAVDVPVTLIGTGAGIDDIIDLR"
     gene            1384..1845
                     /locus_tag="VGA92_00945"
     CDS             1384..1845
                     /locus_tag="VGA92_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845011.1"
                     /translation="MSEPNYAGNIIISLASLPDFLRKPILKKRMDEFFTLSAPDRLEI
                     INNALEAGPTIPFPNFSKLFSTWLEILATNPEEKRTVMFSAYISEILKNPQKLVSFNL
                     DGILEIYVSLDSEKKQTIANSIRQIISRLSDEEKRTLYLIIPQNAKAHLGI"
     gene            complement(1842..2120)
                     /locus_tag="VGA92_00950"
     CDS             complement(1842..2120)
                     /locus_tag="VGA92_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEX9845012.1"
                     /translation="MTKVCTKCKSEIPDSEQLEPTAATYPCCTKCWNEWKEYRIMVMN
                     ELRLDMSLPDHRKLLKKNEKIFVGVLSPQGDIIDFRNEDNRKPDEPQA"
     gene            2197..2406
                     /locus_tag="VGA92_00955"
     CDS             2197..2406
                     /locus_tag="VGA92_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845013.1"
                     /translation="MKIKCPKCKGIAELESDFSLVRCQNCDLEMTYGEYVKYVAHNDV
                     TYSDILGDYTGTTAGQNAGTLDEWD"
     gene            2438..>2984
                     /locus_tag="VGA92_00960"
     CDS             2438..>2984
                     /locus_tag="VGA92_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845014.1"
                     /translation="MVELFFLENILNLVGFAVGLVIGIISYIGFKNTGSPTLFRLTIA
                     FFSIGVGFMIMWLGQTLDVFVYKTDQPTRWMQTLGIGSQTVGYFFIAFSHTIKSFFPK
                     SNYLRSIGVVPLFLISFISVENIIRSISFILLVYGAIETIMSYFESKRRTALFVAAGL
                     GALAFGEFLGWYYFVFPQSILN"
BASE COUNT          775 a          659 c          616 g          934 t
ORIGIN      
        1 cctgcctcct cctttccgtt tctaaatcca tacgagatgt aatgcccgtt gtgctcgatt
       61 ggcacatagt agattactgg gcgctccttt agcagatcca tctgaatggc tagaattttt
      121 ttgccatcat gctcatgaag aaatgatact cgcggtgctc gctcaagggc acaaaccaag
      181 cgtgaaaatt ccatatgcga ggcaagttga acgtattttg gagattttat tttttccaca
      241 gactctgtaa agtaaatggc aaattaaagc ttagcaaaaa aaattcaaac aatcaattaa
      301 cgcaagtcga taatgtcgtc gatgcctgca ccagtaccaa tcagtgttac aggcacgtca
      361 accgcatcct cgattttcgt tatgaatgat tttgctcgat cactcagatc ctcaaacgaa
      421 tttttgtgcg cacattctgg aaacatcacg tcaagcttag ttatcgcaat ctgcgtagcc
      481 gagttgagca tgattgcacg cttggccata ttgaaattaa attcggctgc gcgtctttgt
      541 ctgcctgtta ctgtgcctac ctccgaccag cctcgtgcgg caacctcctc tgcaggcagc
      601 tcgttttcaa ggtggcccgt gcctacgcgt gtcacgtaag acttgaagac gaccattacg
      661 tcatcaacgt tctttgggcc aaggccaatg tctgcacaaa ttcctgaggc tgtgacgtcc
      721 tttgaggtca caaatgggta cgtcccatgc caaagcgaaa gaaaagttcc ctgtgttccc
      781 tccacgagca ctgtttcatc gtttgagagg gccagattaa tctgctctgc gacatctgta
      841 agatatggtt tgagcgagtc aatgtctttt gcaagcttga gcgtacgcat ggccctgtct
      901 gcatttgcag ggccagttcc agaaccagtg cttccaattt tttcttttag tcttcctgcc
      961 gagtctgcat cggtgtgaga tttttcaatt atgccgcagc tagaatctac aaatgttctt
     1021 tttgccactc caaattcttc gatttctttg tgcaaaacat ccggattgac aacgacccca
     1081 gggcctatca tgagttttgc ttttttgttc aaaaatccac ttggcaacat tctgactttg
     1141 tattttttgt tgccatcctc aattgtgtgg ccggcgtttg ggcctgcacc accccttacc
     1201 acaattgtcg gatcgtcttt ttgtgccaag tacgagatta tcttgccctt tccctcatcg
     1261 ccaaagaatc caccaactac taccatcgaa ggcatgtctt gctcacattt tcgacttaat
     1321 ttaagctaat tattggtgat cggtcaaaat agaattttat tggtatggat ctggacaaaa
     1381 cttgtgtcag aaccaaatta tgccggaaac attattatca gtttggcaag tctgccggac
     1441 tttctaagaa agccgatttt aaaaaagaga atggacgagt tctttacctt atcggcacca
     1501 gacagactag agataatcaa caatgctctg gaggctggtc caaccatccc gtttccaaat
     1561 ttttccaagc ttttttcaac atggcttgag atccttgcaa ccaaccctga ggaaaaaaga
     1621 accgtcatgt tttctgccta catctctgag atcctaaaga acccacaaaa actagtctcg
     1681 tttaacttgg atgggatact tgagatctat gtatccttag attctgaaaa aaagcagacc
     1741 attgcaaact ccattaggca gataatctca aggttgtctg atgaggaaaa aagaacgctc
     1801 tacctgataa ttccacaaaa tgccaaagcc cacctcggaa tttaggcttg gggctcgtct
     1861 ggctttctgt tgtcttcgtt cctaaagtcg attatgtccc cttggggaga caaaacgcct
     1921 acaaagatct tttcgttctt tttgagaagc tttcggtggt ctggcaacga catgtccaat
     1981 cgtagttcgt tcattaccat gattctgtat tccttccact cgttccagca ttttgtacaa
     2041 cacgggtagg ttgccgccgt tggttcaagc tgctcagagt cagggatttc agatttgcat
     2101 ttggtgcaaa ctttggtcat tgtttattca acccttttgc tgtcaattta ttcttttttt
     2161 ggcaacggta ataaggcaaa atgagatata tcttgtgtga aaatcaagtg cccaaagtgc
     2221 aaaggcatag cagagctcga gtctgatttt tcgctcgtac gatgtcaaaa ttgtgatttg
     2281 gagatgacgt atggtgaata tgtcaaatat gtagcacata acgacgtcac atattctgat
     2341 attttgggtg attatacagg aaccactgca gggcaaaacg ccggaacgct cgatgagtgg
     2401 gactagtttt aaaaatcaaa ccaaattata gatagcgatg gtagaattat tttttcttga
     2461 aaacatatta aatttggttg gctttgcagt gggccttgta attggtataa tttcctacat
     2521 tggttttaaa aacactggca gtccaactct gttcagacta acaattgcgt ttttttcaat
     2581 cggtgttggt tttatgataa tgtggcttgg tcagacactt gatgtgtttg tgtacaagac
     2641 agatcagccc acccggtgga tgcagacact tggcattgga tctcagacag taggttactt
     2701 tttcattgca ttctcccata ccattaaatc attttttcca aaatcaaact atcttcgttc
     2761 cataggagtg gttccactct ttttgatttc gtttatttca gtggagaaca tcataagatc
     2821 gatttcgttc atcttgcttg tatacggcgc aatagaaacc atcatgtcgt attttgagag
     2881 taagcgaagg actgcgcttt ttgttgcggc aggacttggc gcacttgcgt ttggcgaatt
     2941 tcttggctgg tattattttg tatttccaca atcaattctt aacg
//
LOCUS       DASWKH010000011         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_23277, whole genome shotgun sequence.
ACCESSION   DASWKH010000011 DASWKH010000000
VERSION     DASWKH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23277"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VGA92_00965"
     CDS             complement(<1..672)
                     /locus_tag="VGA92_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9845015.1"
                     /translation="MIDIIFSGFIYYYDKDLVWGQIVDQISKPILDKIFAAPSESELI
                     IQDFAVIMIIASIMALISYKLKQPMIMAYIIAGMIIGPHTPPFSLIVHVDILHVFAEI
                     GIILLLFVIGMEFPIEKLKNIGKKATFIAVAEASGTFVAGYFVSQALGFGFFDSLFLA
                     LAISVTSTVIIMRVLEELGMINDEATYLIVGIAVIEDIIIVSLLAVLQSVAATGDLSF
                     VEVSVS"
     gene            726..1544
                     /locus_tag="VGA92_00970"
     CDS             726..1544
                     /locus_tag="VGA92_00970"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX9845016.1"
                     /translation="MKKIGLLGCGAIGTQIALAIDSGKIPAELTHVYDSDKAKSEILA
                     SKLKKKPIIVDNPHLLSSNPVDIVVEAASQDAVRNHALSIIQNRKDLMIMSVGALLDE
                     SVLEILLFACKEFNKKIYLPSGAIAGLDAIKSVKAELESVTLTTTKNPKSLAGAKFFE
                     TNKIDLGSITKPTVIYEGNAKDAVKLFPANINVAALLSLAGLGSEQTHVKIVADPAAD
                     KNIHQIEAKGKFGNLAINVQNVPDLGNPKTSRLAILSAIECLRLACSDDIRIGT"
     gene            1636..2571
                     /gene="nadA"
                     /locus_tag="VGA92_00975"
     CDS             1636..2571
                     /gene="nadA"
                     /locus_tag="VGA92_00975"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX9845017.1"
                     /translation="MLADKILELKKKKDIVILAHNYQLPEVQDVADFVGDSLGLSRQA
                     AKTKSKTILFCGVHFMAETAAIICPDKKVLIPDLAAGCSLSDSITADQLRVWKKEHPD
                     AVTVGYVNTTAEIKSELDYCCTSSNAVNVVNAIPKSREILFLPDMFLGSYVAKVTGRK
                     NMYIWAGECHVHAGISSEDIQKKLGLYAKSELLVHPECSCTSQIMYDVAVGDYKNRQV
                     QILSTEGMMNYAKESAAKNFVVATETGILYRMQQQNPDKTFVPASRAAVCQYMKMITL
                     DKVYDSLLEDKYEVRVPKQIADKARLAIERMLAIS"
     gene            2685..3101
                     /locus_tag="VGA92_00980"
     CDS             2685..3101
                     /locus_tag="VGA92_00980"
                     /inference="COORDINATES: protein motif:HMM:NF042555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX9845018.1"
                     /translation="MSPLTVTDLSVFNEICKRIMSEPGVRFAGIVNQMGNRIAGGFRE
                     DVVPFVDYTKENMMYMELVLDLRMRGEFDGELGKVQYIHSRRDKVNMVSIPLGNLIAV
                     LATELEVNVQRLVNLAINEFKTKSDTNLSFDQFNVS"
BASE COUNT         1021 a          627 c          665 g          809 t
ORIGIN      
        1 tgaaacagaa acttcgacaa aagagagatc acctgtggca gctactgact gcaagactgc
       61 aaggagtgat acaatgatga tgtcctctat tacagcaatt ccaacaatca ggtaggttgc
      121 ctcgtcgttt atcatgccaa gctcttcgag tactctcatt atgattacag tacttgtaac
      181 agaaattgca agcgccaaaa acaggctgtc aaagaaccca aagcccagtg cttgtgatac
      241 aaagtaacct gccacaaagg tgcctgatgc ctctgcaact gcaatgaatg ttgccttttt
      301 accaatgttt ttgagctttt cgattgggaa ttccattcca attacaaaca acagtaggat
      361 gattccaatt tctgcaaaga catggagtat gtcgacatgt acaatcaagc tgaatggcgg
      421 tgtgtgtggt ccaatgatca tccctgcgat gatgtatgcc atgatcatcg gctgctttag
      481 cttgtatgag attagcgcca taattgacgc aatgatcata attactgcaa aatcctgaat
      541 tatcagctca gattctgatg gggcggcaaa aattttgtct aaaattggct ttgatatttg
      601 gtcaacaatc tgtccccaca caaggtcttt gtcgtaataa tagataaatc cactgaaaat
      661 tatatctatc aaaaaattcc tgattctaat acttcattta ctcctttgat gttttgaaac
      721 ttggtatgaa aaaaattggg cttttggggt gcggcgcaat tggaacgcaa attgctctgg
      781 cgatagattc gggcaaaatt ccagccgagc tgacgcatgt gtatgattct gataaggcaa
      841 aatccgaaat tctggcatca aagctaaaga aaaagccaat tattgttgat aacccccacc
      901 tcttatcctc aaacccagtg gatattgtag tggaggcggc atctcaggac gcagtcagaa
      961 accatgccct gagtattatt caaaacagaa aagacctgat gataatgagt gtaggtgccc
     1021 tactagatga atcagttttg gaaattcttc tttttgcatg caaagaattt aacaaaaaga
     1081 tctaccttcc atctggtgca attgcaggcc ttgatgcaat aaaatcagtc aaagctgagc
     1141 tggaatcagt aactcttact acaacaaaaa acccaaaatc acttgccgga gcaaagttct
     1201 ttgagacaaa caaaattgat cttggctcca tcacaaaacc cactgtaatc tatgagggaa
     1261 acgcaaaaga cgcagtcaag ctgtttccag caaacatcaa tgtcgcagcg cttttgagtc
     1321 ttgcaggcct tggaagtgag cagacgcatg tcaaaattgt cgccgacccg gcagcggaca
     1381 aaaacatcca ccaaattgag gcaaaaggaa agtttggaaa tctggcaatt aatgtgcaaa
     1441 acgtcccaga tctgggcaac ccaaagacta gcaggcttgc aatactgtct gcaatcgagt
     1501 gtcttaggct ggcatgctct gatgacatcc gaataggaac ataatggcaa aaattgccgt
     1561 aatagtacag ttttctgaaa aaatcaactc taatctcatt ctttaaatga atacacaaag
     1621 caagctttag atcgtatgct tgctgacaaa atcttggagc taaaaaagaa aaaagatata
     1681 gtaattttgg cacacaacta ccagctgccc gaagtccaag atgtggcaga ctttgtaggc
     1741 gactctttag gattatcaag acaggcggca aaaaccaaaa gcaagacaat cttattttgt
     1801 ggcgtacatt tcatggcaga gactgccgca ataatttgcc cagacaaaaa agtcctaatt
     1861 ccggatttag ctgcaggctg ctcgctttct gactccataa ctgctgatca gctgcgtgta
     1921 tggaaaaaag aacacccaga cgcagtaact gtgggatatg tcaatacgac tgcagaaatc
     1981 aagtccgagc ttgattattg ctgcacatca tcaaatgcag taaacgtcgt aaatgcaata
     2041 ccaaaaagca gagaaatctt gtttttgcct gacatgtttt tgggctcgta tgttgccaag
     2101 gtcacaggta gaaaaaacat gtacatatgg gcaggtgagt gccatgtgca cgcaggaatc
     2161 agttcagagg acatccagaa aaaacttggc ttgtacgcaa aatcagaact tttggttcac
     2221 ccagagtgca gctgcacatc acaaataatg tatgatgtgg cagtaggtga ctacaaaaac
     2281 aggcaggtgc aaatcctctc aacggaagga atgatgaact atgcaaaaga atctgcagca
     2341 aagaactttg ttgttgcaac agagactgga atcctgtatc gaatgcagca gcaaaaccca
     2401 gacaagacgt tcgttccggc atcacgtgcg gcagtgtgcc agtacatgaa gatgataaca
     2461 ctggataagg tctatgactc attacttgaa gacaaatacg aagtacgtgt tccaaaacaa
     2521 atagcagaca aggcacgact tgcaattgag cgtatgcttg caattagcta accaataact
     2581 taggaattta ccactttcac taaaaactgt aaaaaactag caaaccagat gttttgagat
     2641 catcagctaa acttttaact taagaagtgc tatttgttgc gaccatgtct ccattaaccg
     2701 ttacagatct ttcggtattc aatgagatct gtaagagaat aatgtcggaa ccaggagttc
     2761 gttttgctgg cattgtgaat caaatgggaa acagaatagc aggtggtttc agagaagacg
     2821 tggtgccatt tgttgattat acaaaagaga atatgatgta catggagctg gtcttggact
     2881 tgaggatgag aggcgagttt gacggcgagt tgggaaaagt gcaatacatc cactcaaggc
     2941 gggacaaagt caacatggtg agcattccac ttggtaatct tattgcagtg cttgcaactg
     3001 agctggaagt aaatgtgcaa cgcctagtta atctggcaat aaatgagttt aaaaccaaat
     3061 ctgatacaaa tctgtctttt gatcaattca acgtgtccta atattggatt ctgttcaaaa
     3121 at
//
LOCUS       DASWKH010000012         9708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1873060, whole genome shotgun sequence.
ACCESSION   DASWKH010000012 DASWKH010000000
VERSION     DASWKH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873060"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VGA92_00985"
     CDS             complement(<1..594)
                     /locus_tag="VGA92_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX9845019.1"
                     /translation="MPKVLVIKNTGREGPGTIGGLFASDGYDLDVVFAKKQKIPPLDH
                     DVVLILGAPQSANDSRGYLKDEMSLIREAEKKNIPTLGICLGSQLMARAFGARVYAGP
                     KKEIGFYHDIRIDQDSKSRLFDGIADPFTVFHWHGDTFEIPKNAIRLAHSEMYNQAFR
                     YGSAVGVQFHFEVNKEIILSWLDNAQDLNLSYIDPAKI"
     gene            complement(594..1115)
                     /locus_tag="VGA92_00990"
     CDS             complement(594..1115)
                     /locus_tag="VGA92_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845020.1"
                     /translation="MANKHTIVVAVSIIVIAASLGYSSLNLLSAKDLQFRWYQPDSFD
                     LLSIIFGGKLAVCNNSDYPAAFASYSFDVIYEGQSLGVFTANGAGLAPHTNTMVDGKF
                     ATDNKQVANILFASLDTALGGGQAARINPNNMQVITTLETKIIGLVPFSLTHQYSGEE
                     FVKMMSQKTECDE"
     gene            complement(1174..2445)
                     /locus_tag="VGA92_00995"
     CDS             complement(1174..2445)
                     /locus_tag="VGA92_00995"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX9845021.1"
                     /translation="MVQGDPWKNALKQLDDACQILKIDKGTRDYLAEPNKVLRVKLPV
                     KMDNGKIRVFTGFRSQHNNDRGPYKGGIRYFDPEGGIDYMEKEVKALSSWMTWKCAVV
                     DVPLGGGKGGIYVNPKKEKLSENELERLTRRFAFAISEIIGPKKDIPAPDVYTTGKEM
                     SQIMDVYSKLSGMESTPGVITGKPLSLGGSLARNVATGLGCAYCVREAAKTIGLKLRG
                     AKVVIQGFGNAGTFTAEYLEKMGAKVIAVSDTKGSIINPKGLDSKKVLAFKNKTGSVV
                     GYPGSKKISTEQLLTTKCDILVPAALENQINASIAKKLQCKIIAEAANGPTMPEADPI
                     IYSKKIIVIPDILANSGGVCISYLEWVQNNMGYYWTFDEVASKMEHHIVRGFKDTLAL
                     SKKHKIDMRRAAMVLAVGRVVEAFKAKGLWP"
     gene            2758..3045
                     /locus_tag="VGA92_01000"
     CDS             2758..3045
                     /locus_tag="VGA92_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX9845022.1"
                     /translation="MADLKIKLSIHARERAVSRGIDENEIRRIVVNPIEEVFDEENLN
                     YKCYGQAMDHYTKQTRYLMVVHSGKFNNSVMVITSMWIDPRRLKFYGFSKV"
     gene            3026..3247
                     /locus_tag="VGA92_01005"
     CDS             3026..3247
                     /locus_tag="VGA92_01005"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX9845023.1"
                     /translation="MDSVKFSREAKALYVKFDKENRRIAETIPIGQDRFLDIDEKGNV
                     LGIELLFPKNMPEEVYEAIIRTDSIEITS"
     gene            complement(3367..3801)
                     /locus_tag="VGA92_01010"
     CDS             complement(3367..3801)
                     /locus_tag="VGA92_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845024.1"
                     /translation="MSKSKVPVKGFMQKNVATISSGSSIKEAAKIMYEKKIGSLVIED
                     GGELRGIVTERDISRSLFVYDVPSDSRVKDIYSTPLVSVDPDSSILDAADMISKTQIL
                     RIPVIKDGKLLGIISATDLAVLFSMFENEELEKKLGPYVSEL"
     gene            3975..4346
                     /locus_tag="VGA92_01015"
     CDS             3975..4346
                     /locus_tag="VGA92_01015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX9845025.1"
                     /translation="MSSDKITQFTKHKYINLETYKKSGQPVQTPVWFVIDGGQIFVTT
                     RPDTGKVKRLKNNQSVKIMPCGMRGDPKGEWISGAARFANETESENAVKLRNKRYGMR
                     AKMVGMFAYRGIKPTVIAISV"
     gene            complement(4343..4696)
                     /locus_tag="VGA92_01020"
     CDS             complement(4343..4696)
                     /locus_tag="VGA92_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9845026.1"
                     /translation="MIQEKSGSATHRPDRDARRLLLYVFTATRGGYTRIQIIRLLSEN
                     SMNTNQLATELGLDYKAIQHHIGVLEKNNLVTKIGAKYGATLSLSNYLEANILALDEV
                     VTKLERQLSRKIVYL"
     gene            4850..5581
                     /locus_tag="VGA92_01025"
     CDS             4850..5581
                     /locus_tag="VGA92_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HEX9845027.1"
                     /translation="MMPARGYDMTPTMYSPDGRIYQVEYAIETVKRGTLAIGIKSKDG
                     IILAVEEIPRKLQVSGLTQKIFLVDDHIGVAAAGYIPDARVQVDDARMFSQSHKLIYD
                     EQVDVETVAKHLADRCHQYTQYSGVRPFGVALIIAGVDPHGNIIYVTDPSGTFVSYAA
                     VAIGAGAEEVNTFLEKNYKDDMSLDDASALAIAAIELKSEQGDGIKHIKMARITKDKK
                     NLEKFSDADLEKYSSMAKTKFPKSP"
     gene            complement(5582..5956)
                     /locus_tag="VGA92_01030"
     CDS             complement(5582..5956)
                     /locus_tag="VGA92_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845028.1"
                     /translation="MPRAIVVDDDRDTVDVFCEYLRLIDVDVVGWGYNGKDAAELYEK
                     HRPDIAFVDLSMPEHDGFFALEGIKGLDPGARVVILTANYTDDIVRKLEQLKPHKILK
                     KPFGTEQVYEIINEIRQSIVMV"
     gene            complement(6095..7294)
                     /locus_tag="VGA92_01035"
     CDS             complement(6095..7294)
                     /locus_tag="VGA92_01035"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9845029.1"
                     /translation="MPVKSTRQNYKPNSEQLQMMEIFTQMVNTCIKIGLDNHCSTLKR
                     LSLLSYQKLAKYDIMSYYKINAISQAVGRLQQRKQSIKRGVKTKSPFVQRPFLVSYYG
                     FKINGCLLTIPYKPRQPIHILLNEHTQKILSDSALKMNSFSMSQNSLGLCISKQVQKI
                     RPENIIGIDRNLRNVTVGNNDKVIFFKTNKLLSIKENSMHARAGFKRNDHRMKRRFYQ
                     KFNQRVQHRTKKLVHKISKQIVEKAKQNKSAIIFENLKGIRKLYRKGNGQGNKYRRKL
                     NGWPYYELQHQVEYKAAWDGVPVRFEDPKCTSKLCPVCGKRIQEDRQNRRKLWCSNCM
                     KLMDRDVVASLNIAYKGWARFTHPRGDTGEAQSGIFEPAMSEPKISNYDSLVIRIVDV
                     SKGESWN"
     gene            7440..8129
                     /locus_tag="VGA92_01040"
     CDS             7440..8129
                     /locus_tag="VGA92_01040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9845030.1"
                     /translation="MGLGSYWGEVIEVLRQIIPVYDKVNSYISLGKDVEHRNRGIKGR
                     VNQGDTILDAGSGFGNMSRTAEKLCSGNLSITLYDPLVPMLKNAGTRFAKTPELSCGV
                     FEHIPFKEEHFDAVLCGYSLRDAINLRIAISEIHRVLKEGGRFVIVDLGKPDNSLIRA
                     GVSFYLRAVLPIIAFCVGGRLGLKFATLYGTYRRWPQNKKLESLLLEKFSRVEFEKDL
                     MGGAIMVAAYK"
     gene            8126..9277
                     /locus_tag="VGA92_01045"
     CDS             8126..9277
                     /locus_tag="VGA92_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9845031.1"
                     /translation="MNKTIVLINIVGLIIGISYGLHNPIVPIFAKNEIGASYAELGLI
                     GLANFVPYMFIPVFVGILLGRINNGHLLSLGILINASSIFLLSVSKSVPEMMVLRAMT
                     GVAHAFFWPPAEAIISNSSGGESRVKNIGRFTGFFVSGFMIGPLIGTFLLDGLDISFR
                     MLFEIATFVMAAAIIFSIPLSRHVSPKQDSKFSFSALKEIARFPQIIVILIFCTSAFG
                     TILTIYPAFLNDRSMSATEIEVLYFVFGVSRVITLASTGRLARHTGGTLIASTLSIAA
                     GLFVSFYSHSILEFAAAMLLMGFGFSIIFPLTLEIVLRRTKKEASGKMIGAYETTFGM
                     GWTSGPIIAGLISEFSGNAMPYMVFFAIGIGVAILSIIKRKSLDPLANP"
     gene            complement(9298..>9708)
                     /locus_tag="VGA92_01050"
     CDS             complement(9298..>9708)
                     /locus_tag="VGA92_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEX9845032.1"
                     /translation="CILDGPNKQQLLSGCLIATATYGTEFAPQVQQLREIRESTLLAT
                     NSGATFLSAFNDAYYSFSPTVADWERQNPAFKALVQTTITPMISTLSILNYVDIDSES
                     EMIGYGIGIILLNIGMYFAAPAFAIIKLKKILYK"
BASE COUNT         2562 a         2116 c         2121 g         2909 t
ORIGIN      
        1 aatctttgca ggatctatgt aggaaagatt caggtcttgc gcattatcaa gccatgacaa
       61 tatgatttct ttatttacct caaagtgaaa ctgtacccct actgcgctgc cgtaccggaa
      121 cgcctggtta tacatctcag aatgcgcaag ccttatcgcg ttttttggaa tctcgaatgt
      181 gtcgccgtgc cagtgaaaaa cagtaaaggg gtctgcaatc ccatcaaaga gtcttgattt
      241 agagtcctga tcaattctga tatcatggta aaagccaatc tccttttttg gcccggcata
      301 gactctggcc ccaaacgccc ttgccattag ctgcgagcca aggcatatgc caagtgtcgg
      361 gatgtttttt ttctcagctt cgcgtatcaa actcatttcg tctttgagat atccccggga
      421 atcgtttgca ctttgcggag cacccagaat cagaaccaca tcatgatcaa gtggtggaat
      481 tttttgcttt tttgcaaaca ccacatccag atcatacccg tctgatgcga aaagcccccc
      541 tatggtgccc ggcccctcgc gccctgtgtt ttttatgact aggacctttg gcattactca
      601 tcacactctg tcttttggct catcattttg acaaattcct cgccggaata ctggtgcgtt
      661 agtgaaaacg gcacaaggcc gattattttt gtctcaagcg ttgtgattac ttgcatgttg
      721 ttcgggttta tccttgctgc ctggccgccg ccaagagcag tatcaaggga tgcaaaaagt
      781 atgttggcta cctgcttgtt gtctgtggca aacttgccat caaccatcgt atttgtgtgg
      841 ggcgccagtc ctgcgccatt ggcagtaaac acaccaaggc tttgtccctc atagataaca
      901 tcaaatgaat agctggcaaa ggccgcagga tagtccgagt tgttgcagac tgcaagcttt
      961 ccgccaaaga taattgagag cagatcaaaa gagtctggct ggtaccacct aaactgcaag
     1021 tccttagcag acagaaggtt cagcgacgaa tagccaagcg aggccgcaat tactatgatt
     1081 gaaactgcca caactatggt gtgcttgttt gccacaagtt acttggaaaa aaaggcgtaa
     1141 ttttatgtat tgacaaaatg aaagtggtgg gttttatggc caaaggcctt tagctttgaa
     1201 tgcctcaacg actcgaccga ctgctagaac catcgctgcg cgcctcatgt cgattttgtg
     1261 ctttttggat agtgcaagtg tgtccttgaa tccccgcact atgtggtgct ccatttttga
     1321 tgcgacctca tcaaatgtcc aataatatcc catgttgttt tgcacccact ccaaatacga
     1381 tatgcatacg ccgccagagt ttgccagaat atccggtatg actataattt ttttgctgta
     1441 gatgattggg tctgcttccg gcatcgtagg tccgtttgca gcttcggcaa ttatcttgca
     1501 ttgcagtttt tttgcaatcg atgcgttgat ctggttttct agtgccgcag gtactagtat
     1561 atcgcacttt gtcgtaagca gctgctctgt ggaaatcttt ttgcttccag gatatcccac
     1621 aacagagcca gtcttgttct taaatgcgag aaccttcttt gagtcaagcc cctttggatt
     1681 gattatagag ccctttgtgt cgcttaccgc aattactttt gcgcccatct tttcgagata
     1741 ttcggcagta aaagttcctg cgttgccaaa tccctgtatg actaccttgg cacccctcaa
     1801 cttgaggcct attgtctttg ctgcctcgcg cacacagtat gcgcacccaa ggcctgttgc
     1861 aacgtttctt gcaagcgagc ctccaagtga gagcggcttt ccggtaatta ctccgggtgt
     1921 gctctccatt ccggacagct tcgaatacac gtccattatc tgcgacattt cctttcctgt
     1981 cgtgtagacg tcgggtgcgg gaatgtcttt ttttgggcca atgatttcag atattgcaaa
     2041 ggcaaatctc ctagtcagcc tttcaagctc gttttcactt agcttttctt tttttggatt
     2101 gacatagatt ccgccctttc cgcctccgag tggaacgtcg actactgcgc acttccaagt
     2161 catccacgag gaaagcgcct tgacttcttt ttccatgtaa tctattccac cctctgggtc
     2221 aaagtatctg attcctccct tgtatgggcc cctgtcgttg ttgtgctggc ttctaaatcc
     2281 cgtgaagact cggatcttgc cgttgtccat cttgacagga agctttactc gcagcacctt
     2341 gtttggctct gcaagataat cccgggtgcc tttgtctatt tttaagatct gacaggcatc
     2401 atcgagttgt tttaatgcgt tcttccatgg atctccctga accaaattga tcgagatcta
     2461 ctgaagccag ttcgtattta agtttgacta gaaatgatgc tgtaaagtct ccgtcggttt
     2521 ttattcgtgt caaatgtcgg ttggtcgatc aagaaaaaat agtgttacat tttgattgat
     2581 tgttccaact tacattgatt gcactaacaa ggttgctatt tttggggaaa ttatgtaaac
     2641 aaatttctgc ttatctactg ttagcataat tagagaaagt gtcgcttcga tttaatggat
     2701 acgaacgatc agaaagacag aagaagataa caagtagatt attcaatcta attcttgttg
     2761 gcagacctta aaattaaact gagcatacac gcaagagaaa gagcagtatc tcgaggcatt
     2821 gatgagaatg agatcaggcg aatagttgta aatcctattg aggaggtttt tgacgaggaa
     2881 aatttgaact ataaatgcta tggtcaagcc atggatcatt acacaaaaca gacacgttac
     2941 ttaatggttg ttcacagtgg taaatttaat aattcagtta tggtcataac atcaatgtgg
     3001 atagatccgc gaaggttgaa attttatgga ttcagtaaag tttagtcgtg aagcaaaagc
     3061 attgtacgtc aaattcgaca aggaaaacag acgcatagcg gaaactatac cgattggtca
     3121 agatagattc ttagacattg acgaaaaagg taatgtttta ggcattgagt tattgtttcc
     3181 aaaaaatatg cctgaagaag tgtatgaagc aatcattaga acggattcaa tagaaataac
     3241 ctcctaattc caaagttttt tacaaaataa aattagattc tgtatgatta acgagacacg
     3301 cctgaatttt attcgtgtca agtctcagtt gatggatcaa gaaaaatctg aaacttgaga
     3361 tcacgtctat aattcactaa cgtatggacc taattttttt tctaattcct cgttttcaaa
     3421 catagaaaat aacactgcaa gatcagtggc ggaaataatc cctaataatt ttccatcctt
     3481 tatgaccgga attctaagaa tctgtgtttt gcttatcata tccgcagcat ctaaaattga
     3541 agaatctggg tctacagata caagaggcgt tgaatagatg tcttttactc tggaatcgct
     3601 tggaacatca tacacaaaca aggacctaga gatgtccctt tcagtaacta ttcctcttag
     3661 ttcaccacca tcttctatta ccagagaacc aatctttttt tcatacatta tttttgcagc
     3721 ttccttaatt gaagaacccg aactgattgt tgccacattt ttctgcatga atcctttaac
     3781 tggcaccttt gatttactca agttgaggtt atttatcaaa ccaagtataa taatggacag
     3841 tctaaatctc aattaagaaa atcaatctag taaagtgaga tatcaaccga tgtttcgcag
     3901 gcatacgatc aactacagaa ctgatagagc cagaataatt gaactaaaat aatcgaacag
     3961 attatcatag tataatgagt tcagacaaga tcacacaatt tacaaaacac aagtacatca
     4021 acttggagac ctacaagaaa agtggtcagc cagtccagac gccggtttgg tttgtcatag
     4081 atggcgggca gatttttgtc acaacgcgtc cagacacagg caaagtaaaa cgcctcaaaa
     4141 acaaccagag cgtcaagata atgccgtgcg gaatgcgagg agatccaaag ggggagtgga
     4201 tctcaggggc tgccagattt gcaaatgaga ccgagtcgga aaacgcagtc aagctccgaa
     4261 acaaaagata cggaatgcgc gcaaagatgg taggaatgtt tgcctacagg ggcatcaagc
     4321 caacagtgat tgcaatttct gtctaaaggt agactatttt gcggctcagc tggcgctcaa
     4381 gctttgttac tacctcatct agtgccagaa tgtttgcctc aagatagttt gagagcgaaa
     4441 gtgtcgcgcc atattttgcg ccaatttttg tgaccagatt gtttttttcc aataccccga
     4501 tgtggtgctg aattgccttg tagtctaggc ccagctcagt tgcaagctgg tttgtgttca
     4561 ttgagttttc tgataaaagc ctaattatct gaattctagt atagccgccc ctagttgcag
     4621 taaagacgta gagcagcagc cttcgtgcgt cacgatcggg cctgtgtgta gccgatccag
     4681 atttttcttg gatcatttga cggacctcta ctagtgtttc tgcgctcatc agattccagt
     4741 aatgcagttt tgatttaaaa cactatttcc aaatctcttc caaattacac caaaattttg
     4801 aggctcgcaa ctaacgctta aattgacaac aaagtttagc tgaatcatta tgatgccagc
     4861 gcgcggctac gacatgacgc ctactatgta ttccccagac gggaggatat accaagtaga
     4921 gtacgcaata gagacagtaa agcgcggaac gctggcaatt ggaatcaagt ccaaagacgg
     4981 gatcatcttg gcagtagagg agatcccccg aaagctccaa gtctccggtc tcacccaaaa
     5041 aatcttccta gtagatgatc acatcggggt tgcagcggca ggctacattc cagatgcacg
     5101 agtccaagta gacgatgcgc gaatgttttc gcaaagccac aagctgattt acgatgagca
     5161 ggtagatgtg gaaacggtgg caaaacatct agcagacaga tgccatcaat acacccagta
     5221 ctccggcgtg cgaccgtttg gtgttgcact aataattgca ggagtcgacc ctcatggaaa
     5281 cataatctat gtcactgatc cttcaggaac ctttgtctca tatgcggcag ttgcaattgg
     5341 cgccggtgca gaagaggtaa acacgttttt ggaaaaaaac tacaaagacg acatgtcact
     5401 tgacgatgcg tctgctttgg caattgcggc aattgagcta aaaagcgagc agggggacgg
     5461 aatcaagcac ataaagatgg cccgcatcac aaaggacaaa aagaatcttg aaaagttcag
     5521 cgatgcagac ttggaaaaat actccagcat ggcaaaaacc aagttcccaa aatcaccata
     5581 gtcacaccat tacgattgac tgtcggatct catttattat ttcatagacc tgctctgttc
     5641 caaaaggttt tttgagtatt ttgtggggct ttagctgctc aagctttctt actatgtcat
     5701 ctgtgtaatt ggctgtaagt attacaactc ttgctcccgg atccaggcct ttgatgccct
     5761 ctagtgcaaa aaacccgtca tgctctggca ttgagaggtc cacaaaggca atatccggcc
     5821 tgtgtttttc atacagctca gctgcgtcct ttccattata cccccatcct accacatcca
     5881 cgtcgatcag tcttagatat tcgcaaaaaa catccactgt gtccctgtcg tcgtcaacta
     5941 cgattgctct tggcattttg tgcacaggat cgattgggtg tgaatttatt tgttaactag
     6001 tgtgttacaa gcggacagat aggtagccat ttggtatgat ggtatgatgg ctagcaggca
     6061 gtgtttacta ggttctgttc aaaagccggc atttttaatt ccaactctct cccttcgaca
     6121 catcgactat tcggattacc aaactatcat agtttgatat tttgggttcc gacattgctg
     6181 gctcaaaaat gccagactgc gcttcacctg tgtcgcctcg aggatgggta aatctagccc
     6241 accccttgta tgcaatgttc aaagacgcca ctacgtctct gtccattaat ttcatacaat
     6301 tactgcacca tagttttcgt ctgttttgcc tgtcctcttg gattctcttt ccgcataccg
     6361 gacagagctt gctggtgcat tttggatcct caaaccgtac cggcactcca tcccaagcag
     6421 ccttatactc tacttggtgc tgtaattcgt agtatggcca gccgttgagc tttcgtctgt
     6481 atttgttgcc ttgaccgttt cctttcctgt atagttttct tattcccttc aaattctcga
     6541 agatgattgc tgatttgttt tgctttgcct tctcaactat ctgttttgat attttgtgga
     6601 cgagtttttt agttctgtgc tgcactctct ggttgaattt ctgatagaac ctacgtttca
     6661 ttctgtggtc gtttcttttg aaaccagctc ttgcatgcat gctattttcc ttgatagaaa
     6721 gcagtttgtt ggtcttgaag aatattactt tgtcattatt tcctactgtg acattcctga
     6781 ggtttctgtc tattccgatt atgttttctg gcctgatttt ttggacttgt tttgagatac
     6841 aaagacccaa gctgttctgg ctcattgaaa acgaattcat cttcagggct gaatctgaga
     6901 gaattttttg tgtgtgttcg ttgagcagga tgtggattgg ttgtcttggc ttgtacggta
     6961 ttgttaacag gcagccgttg attttgaacc catagtaact caccaaaaat ggtctttgta
     7021 caaacgggga ttttgtcttt actcctcttt tgattgactg ctttctttgt tgcagccttc
     7081 caacagcctg tgagattgca tttattttgt aatacgacat gatgtcgtat ttggcaagtt
     7141 tttggtatga aagaagcgat agtcttttca gagttgaaca gtggttgtct agtcctattt
     7201 tgatgcaggt attaaccatt tgagtgaaga tctccatcat ttgtagttgc tcagaatttg
     7261 gtttgtagtt ctgccttgtg gatttgacag gcaacaaatc attttgtcaa ttttagtatt
     7321 aaaccaattt gatgtgtcag gtgttaaccc acgatgcatg gaatttacat cgtgccatca
     7381 aacccaactt ttgaacagaa cggtttacta caaattatcc tgttttgata ccaatccaaa
     7441 tgggtcttgg aagctattgg ggcgaggtaa tcgaggtcct ccggcaaata atcccagtct
     7501 atgacaaggt aaactcgtac atctcacttg gcaaagacgt agagcacaga aaccgcggaa
     7561 tcaagggccg cgtaaatcag ggcgatacaa tacttgatgc gggctctggg tttggcaaca
     7621 tgtctaggac cgcagaaaaa ctctgcagcg gaaatctctc aattacactt tatgatccac
     7681 ttgtgccgat gctaaagaat gccggcacac gttttgcaaa aacaccagag ctctcatgcg
     7741 gagtctttga gcacatacca ttcaaagaag aacactttga tgcagttttg tgtggctatt
     7801 cgctgcgcga tgcaataaat cttagaattg caatctctga aatccacaga gtcctcaaag
     7861 agggcggaag gttcgtaata gtggacctcg gcaagccaga caactcgctc atcagggcag
     7921 gagtatcgtt ttacctcaga gcagttttgc caatcattgc cttttgtgtc ggcggaaggc
     7981 ttgggctaaa gtttgccaca ttgtacggaa cgtacaggcg ctggccgcaa aacaaaaagc
     8041 ttgaatcatt gcttttagaa aagttctcca gagtggaatt tgaaaaggat ttgatgggcg
     8101 gggcaataat ggtcgcagca tacaaatgaa caagacaata gttctaatca acatagtagg
     8161 gctgataatt ggaatatcat acggattgca caatccaata gtcccaatct ttgcaaaaaa
     8221 cgagatcggc gcatcttatg cggagctggg actaatcggc cttgcaaact ttgttcccta
     8281 catgttcatt cctgtctttg tcggcatttt gcttggcaga atcaacaacg ggcacctcct
     8341 ctcactgggg attctgataa acgcatcctc catcttcctt ctgtcggttt caaagtcggt
     8401 tccagaaatg atggtgctca gggcaatgac tggcgttgca catgcgtttt tttggccccc
     8461 tgcagaggca atcatctcaa attcaagtgg tggcgagtcc cgcgtaaaaa acatcggacg
     8521 gttcacggga ttttttgtaa gcggctttat gatagggccg ctaatcggga cgttcctact
     8581 tgatgggttg gacatttcat tccggatgtt atttgagatt gccacatttg tgatggcagc
     8641 tgcgataatt ttttccattc ctctttcaag acatgtatcg ccaaagcagg actccaagtt
     8701 ttcattttcc gcactcaaag agattgcaag gtttccgcaa atcatcgtga tcctgatttt
     8761 ttgcacgtcc gcgtttggaa ctattttgac catctatcct gcgtttttga acgatagaag
     8821 catgtccgca acagaaattg aggtgttgta ctttgtcttt ggggtatcgc gtgttatcac
     8881 gcttgcctca actgggaggc ttgcaaggca cacgggcggc acgctgattg cgtcgactct
     8941 gagcattgcc gctggcctgt ttgtgtcgtt ttactcgcac tcgattttgg agtttgccgc
     9001 agcgatgctt ctgatgggct ttgggtttag cataatattt cctctgacgc tggagattgt
     9061 tttgaggagg accaaaaaag aggcctcagg caaaatgatt ggggcgtacg agactacatt
     9121 tggcatgggc tggacgagcg ggccaatcat tgcggggctg atatcggaat tttctggaaa
     9181 cgccatgccg tacatggtgt tctttgctat tggaatcgga gtcgccatac tcagcataat
     9241 aaaaagaaag tcgctcgatc cccttgcaaa tccgtaaagc ttggaatcag ctataaatta
     9301 tttgtaaaga atttttttca gttttataat cgcaaatgct ggcgcagcaa aatacatccc
     9361 gatgtttagc agaattattc caattccata tcctatcatt tctgattccg aatcaatgtc
     9421 gacatagttt agtatcgaaa gagtcgagat catgggagta attgttgttt gaactagtgc
     9481 cttaaaggct gggttttgtc tttcccagtc tgccacagtt ggcgaaaatg aataatacgc
     9541 atcgttgaac gcactcaaaa atgtcgcccc cgagtttgtt gcaagtagtg tgctctcgcg
     9601 gatttctctt agctgctgga cttgaggtgc aaactccgtg ccatatgtgg cagtggcaat
     9661 aagacatcca cttagcagct gttgcttgtt tggcccatct aagataca
//
LOCUS       DASWKH010000013        73792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3348332, whole genome shotgun sequence.
ACCESSION   DASWKH010000013 DASWKH010000000
VERSION     DASWKH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 73792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73792
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3348332"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4204)
                     /locus_tag="VGA92_01055"
     CDS             complement(<1..4204)
                     /locus_tag="VGA92_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX9845033.1"
                     /translation="MNNAKIISISLIFILLSSFSSSFGDTISQNYKQEITPPKFIEKP
                     SVTELTRDFTLALDEKKLQLTNQEISITLLRQPIVEYITNIHISEDITISNDDGSENQ
                     IYLLKHNRYQAILERIFNYRKFKFDNIVGLPISTGSIVSLYHDIVGSPTNNNDIQNDD
                     FDQILLDINNIFTTDLDTDLLKLQPIIAALAVQSTYDDFGKQIRFITDSFEQQSSTTL
                     FIFTVAAFYIIVRSENPKLKIQTYNRILSLVFISLLASSLIVTPLSISESYYAHAETE
                     KNVNFTDDMPPESLSDQIFEQENILESDIETIIPSSALANITEYPLGETMSESNSTLE
                     LESYENTIISTNTTSITTTNSTNQTTSATFIPTPTNATTPTTPATPLQTVLSLIENLN
                     IIGNSATPTNATTPTILPHIVIPNATKSWNVNATTDSKFVGDVHVNQTGIILDGGFIK
                     SAGNYTNQTSNLAVAAWIKPEYTSGASVFTILSKDKSFELALNNIVHPQRIATFSVFD
                     GIKWHSVQTSTEIGQSWSHIAATFNGTEISIYVNGTISKTVKTIPTITLDSSGNRQNT
                     TPEMAAKDSEIVVGATLDTRTVDTAQKAFTGSLDEVEIYDKYLTAQQILALYQRTLPL
                     ISAKPVPLPPIQIELPPINLLNQTNINGTLATNATEHVIVPTVNQTNKLTISAWVDPN
                     YNNLSDELTVVSKAGSFALSLNNILPPQHTTTFSVFDGIKWTELQGKSKIESLSHLAA
                     VIDGTEISLYVNGTLQEKKTLQNSIEVSEGKLVTSSAEVVKSDTDVVIGAYISILREE
                     PQLSNRFSGTIDDAIIYNRALTEYEIKQIFSQVIGEPRQTFPLFSENILSFTDIVTLI
                     YIPAEVTTFTNSSILNEETIGFADTIALTLIPQNATFVKSHIIDETLGFTDSVTLTLN
                     VTNVLTNATDITKVDTLGFADYVELITNTGLSANGTSNIDALGLVDSISLQKLEALQD
                     SDIAFEERGKDYYKVEFTNGTGFVELGLPEWIYDNATNKYVDHIVAETDTEITVDSMQ
                     IPFEFDKTDCSVSIFEQGRLNKNLAKLVDKYYWKVMESNNGQNWKENAANSAICHVTK
                     TQNSTGTFINALRENEKGIFLTVYGKKIDQPLEVFLYYTNKDANAVSTKIGFVSHMEG
                     LDADEIDFGDEQVATDTKVQEKKIKNQLKKLFNKDAKEEILDTLKELKSSQKLIKSKS
                     QLFKEFALNKEGKKSLLFDFSKAHKEFKQINVENANGKLDTTLEFLDSAQTAEPGETV
                     FLDPVFGFSTGSNIVISTSGASGTSCPSPTTKSSGVGSPILRMRAASQTASPACTRLT
                     TEWNISTIPDDAIIQDTRMRFDISAISGGGRIVDINSMESSPVIGTSPGTIWNDIGDG
                     TTFVTTSNSTVKANDYVEDLGTF"
     gene            complement(4544..5314)
                     /locus_tag="VGA92_01060"
     CDS             complement(4544..5314)
                     /locus_tag="VGA92_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9845034.1"
                     /translation="MGKIVVFDSGFGSLSIIKTIQKRTKAEIIYFADQKNFPYGKKSK
                     RELEKIIKNTINSLQKKFNPDLIVVGSNTPSLLLDKIISNNSRVMGVLPPLLEAQQLT
                     KTNSIAMLVTSSVAKSSALNNFIKKNLIKKIKIIKIDSSDLVELVESGKFIHKKNICA
                     KKIISILQKKFITNNIDVVTLSSTHLPFLLPLLQKLFPHIEFLDPAINVANQLVTHKL
                     FSPAKKNTLKIFSSGDINKFQTNLHKVGIRKPVQQINF"
     gene            5421..6071
                     /locus_tag="VGA92_01065"
     CDS             5421..6071
                     /locus_tag="VGA92_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HEX9845035.1"
                     /translation="MTGFEKNWARQDTASFSDKIKDSVKPQGALKPRIQTAVNKLQLQ
                     ISKMDSMLNKLHERDAQLFKRIVAAMQQHDTSASRVLSNELAEIRKVTKMLSNARIAL
                     EQVQLRLTTIHDLGDAMVAIGPAMSTMKGLKSSLGRFMPEADAELNAMTQTLGGLMMD
                     SLAGDSFSVESDVSSEETERILQEASAVAEQQIGERFPSVPSPSGLSSQSSSSTYE"
     gene            complement(6132..6746)
                     /locus_tag="VGA92_01070"
     CDS             complement(6132..6746)
                     /locus_tag="VGA92_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HEX9845036.1"
                     /translation="MASYNVSDQDGEILVQTARKIVTEFVTNSRKLELDENLRSHLSL
                     ESGLFVTLDLQDELRGCVGFPMPKKLYEALPEAAIAAATQDPRFPPVKSAELDKITFE
                     VTVLTPPVELKVNDPLQLPSRIKVGRDGLIVKQGYRSGLLLPQVPVEYGWSQEEFLDF
                     TCQKAGLPSDCWKDKQTKIFSFEGIIFKEEKPNGKVVREQIHNK"
     gene            complement(6733..7215)
                     /locus_tag="VGA92_01075"
     CDS             complement(6733..7215)
                     /locus_tag="VGA92_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEX9845037.1"
                     /translation="MSQNPSFVIDSMLGTIAKKMRILGFDSKYFATIDDDDLILLAKK
                     ENRAIITKDHQVADNAKKHDILTVDITTHTEKDQLIEIAKKTGFTKYEFNPNIARCPI
                     CNGILHGVEKHQIIDKVPPKIAQNVKEFWICEDCKHIYWEGTHIRNLEKLIAEINGQL
                     "
     gene            complement(7199..9130)
                     /gene="rqcH"
                     /locus_tag="VGA92_01080"
     CDS             complement(7199..9130)
                     /gene="rqcH"
                     /locus_tag="VGA92_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HEX9845038.1"
                     /translation="MALAGIELTFLVGEISKKTSGYYVNNIYSINQDSILFKLHHPQN
                     ADLFLVVSTIGMWLSTIKIDKIEENRLVRRLRNDLIRLKLVKLEQIGLERIAYLRFSG
                     FDKEFVLICEFFGDGNIILCNKDLKILALLHSIEVRHRELQVGMAYTPPPQKGLNILD
                     INEKDFVELKSSSTPIVKWFGRTFGLPSKYAEEILRVAQVDFDTHGSGLTDDKAKEIV
                     TAARALTQRVVSGNHEPVIVKTKDSLDVYPIILNAQDSEYEKIPSFMEGLDKVFSKIL
                     LERGKVSQSSELDKKINDIQSRIDEQNKATSQVKELSEKISSVAKSLFELSSSGITTI
                     IDPAAAEILKSQNTEIVKEKGLDYIKIDGEKIQIRTDASIPAIASTLFDESKRQAAAI
                     ASIESLKAKNQKEIVKLKSQSLDAKGSVSFTQVRKKNWFERYRWFYTSDGFLAIGGRD
                     SSSNSAIIRKHLEKNDKVFHAEIFGSPFFILKDVPESIPFDSLNEVAHATACFSRAWR
                     EAMYGVSAYWINPDQVKKAAPSGQFLSRGSFVLEGQKNFVKVLNLRLAVGILERDGYH
                     LITCGPPEPIKNSCLCYAIIEPGDSEMPEVAKRIRLEFIKLNEDVAKHFTIDDYVRVL
                     PAGGSRIVDVGLSKLEPKP"
     gene            9228..9650
                     /locus_tag="VGA92_01085"
     CDS             9228..9650
                     /locus_tag="VGA92_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845039.1"
                     /translation="MTKHPKKKSPPQDDSKKDEDQPKTEAAENQDEVTRRKKLLDKLF
                     WIRMGLAAMAGVAATFLFESIDGEERRWASIGFMIVTFIASVVIGKGMRIGLPSSDRK
                     KLVTTGIGSYVFIYLFMWILSYTLTHHPDTGLIPLNPL"
     gene            9659..10249
                     /gene="cbiT"
                     /locus_tag="VGA92_01090"
     CDS             9659..10249
                     /gene="cbiT"
                     /locus_tag="VGA92_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HEX9845040.1"
                     /translation="MWKYKTPGIPDEYFDRTEEVPITKEEVRAVQISKARLASGNLVY
                     DIGCGSGSITIEAALQVGEQGKIYAVDIDPKAVELTRKNLAKFQITNAEIILGDAKEK
                     IAALPMADAIFVGGTGGDTKDIVKLCNQKLKSGARIIIGIILIETLYTVLDVIYKLDF
                     GDIDITQITVSKSKKTSTGTMMLARNPVTIISATKN"
     gene            10289..11008
                     /gene="cobI"
                     /locus_tag="VGA92_01095"
     CDS             10289..11008
                     /gene="cobI"
                     /locus_tag="VGA92_01095"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HEX9845041.1"
                     /translation="MHELVCVGCGPGDPELLTVKAVKAIQSAQVIACPTAKEGKPSIA
                     LAVVESIIDKSKPEILNLVFPMVKDKETLESTWEKNTKILAQKVLEGKKVVYLTVGDP
                     YLYSTWIYLHRKMQIKYPEIKIAVIPGIVSMFTFASKVGLSLAEGAETMAVIPSCYDL
                     SRVKETAKNCDTMIFLKDGRYFDQVIKLLKEAGFSDDSIFAIGQDLGTPNEIVRKMRL
                     GDVNENTMTTKYFSIMVVKRV"
     gene            11001..11771
                     /gene="cobM"
                     /locus_tag="VGA92_01100"
     CDS             11001..11771
                     /gene="cobM"
                     /locus_tag="VGA92_01100"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HEX9845042.1"
                     /translation="MYKVYFVGCGPGDPDLITVKAKKLLSKADVVVYSGSLIPQQILK
                     ICKKAKLYDASGLVREEIFEILKNNAQKNKLVIRLHDGDPAIYGAIREQTDNLKKEGI
                     VCEIVPGVTSFLASSAALGLQLTLPGVTQTIIITRAEKRTKVPQQERISELAKHKSTM
                     IFYLSVHLLSDIVKEAIKGGYSKSTPVAIVYKASWPDQKIITGTLEDIVKKVWAEKIT
                     KTAIVMIGDVIQPKSYEYSKLYDKTFSHGFRKAKKNLQ"
     gene            complement(11824..12531)
                     /locus_tag="VGA92_01105"
     CDS             complement(11824..12531)
                     /locus_tag="VGA92_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845043.1"
                     /translation="MKLSTTALILGVALAVVAGLFSSTIFGLVGTKTDAQTNTAATGL
                     FTGHVTTIHEDAAGNVIGYRQSDNLIVNGGENCALKLLFAATGGDASGNTVCVGTNDA
                     GFRWIAIGNTSNAEAGTPAAPLDTDIRLYNPHNSTSYGLENGLARMFGNVNSVTGWTN
                     ATGSTEASVVMSATFTNTAGAARSVSESGLFNATDGNESTDAMFARQVFQTISLNNGD
                     SLTVQWTINVGGTGTFN"
     gene            12769..13449
                     /locus_tag="VGA92_01110"
     CDS             12769..13449
                     /locus_tag="VGA92_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845044.1"
                     /translation="MDKIVFDGKWTHEFEWKASSLDTYHYDNNTEVILRSAHQGDFVY
                     IFLDPITDYQLDEVSDYAIICFDTKNNKSTKADFDDYCFMTYLDSKNSFVYHGGHASE
                     TTSFFNEIPNPEGFIGISTVSDENDRYTSVPHPSYEFRIPTDLIGRESVYGFYFLVYD
                     GHSNKTYTYPQNLDPEGFVSNPSQWGEIYSPDKSLPEFELPLLSLVLAILSVVFFSRI
                     KHRIASTN"
     gene            13454..14119
                     /locus_tag="VGA92_01115"
     CDS             13454..14119
                     /locus_tag="VGA92_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9845045.1"
                     /translation="MRIRSISKLNKQIIKCQKCPRLAAYIRKVAAEKVKRYRHEKYWG
                     RPLGGFGDPNAKLLVIGLAPAAHGGNRTGRMFTGDSSGDWVARVLFGNGFATKATSTH
                     IDDGFSLINAYITAAVKCAPPDNKPTKQEISTCSEYLVQELLVLENVKVIVCLGKIAF
                     DTLKQLLGIKYQKFFHGNKFSFNNKIILCSYHPSRQNTQTGRLTWDQWNKIFLQAKTL
                     LND"
     gene            complement(14127..14510)
                     /locus_tag="VGA92_01120"
     CDS             complement(14127..14510)
                     /locus_tag="VGA92_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX9845046.1"
                     /translation="MTLKVIVKGKTQGKILKTNTPINFLGSVDKKTGVINDNRHDLAG
                     KSIKDTILVFPHGVGSSVGAYTIYSLKSNNSAPLGMVCKKVDLTVASGCALANIPLVI
                     ATEQEYDSLEDGKQITLDAESGTIL"
     gene            complement(14507..15661)
                     /locus_tag="VGA92_01125"
     CDS             complement(14507..15661)
                     /locus_tag="VGA92_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEX9845047.1"
                     /translation="MELTKEEELALKGEYGETLETAYRILIATGEATNAEKLVPIKWA
                     HLSGANYNTIGDAGEEFLSKLSQSAKVKVMTTVNPIGYDFEKVSDYKLSDEFIKKQKS
                     IVQSYQKIGTTPSFSCIPYEIFDLPQKGTQVSFAESNAAIYANSIANLKTNKESSFSA
                     LASALTGKSPYSDLRINDKPNLTIRMKVEQPDEMAFGMLGYFAGKIADNSVAISGVSE
                     MDKRSCKSLCGGMGTSGRCGKFVLGEGTQDSEKVDFDKKEMQKIYDELNTSDRGDLIT
                     LGSPQLGLEEMSSLSLMLKGKSFRKRCMVFCPRAVQEQARNLGYTNELERAGCEILSD
                     CCTCLTPLIDKDDVDSVTTNSIKGAYYLRNSNGVDVNLKPLSQIIKDETK"
     gene            15746..16399
                     /gene="nth"
                     /locus_tag="VGA92_01130"
     CDS             15746..16399
                     /gene="nth"
                     /locus_tag="VGA92_01130"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9845048.1"
                     /translation="MERILSGMINTMNSVKPPRMTALRQLHDVEKGDPFAILIGTILS
                     ARTKDENTTKVVGRLFLQYKTSKDLANAKTKDVEKIIRPIGFYHVKAKRIIDVAKIIE
                     SQYDGVVPDNLDQLVQLPGVGRKTANCVLVYAYEKPAIPVDVHVHRISNRLGLVETKT
                     PEETELALIKKIPKKYWLKINDTFVMYGQNICKPISPMCNVCKIKKYCKYYKTRNAS"
     gene            complement(16383..17054)
                     /locus_tag="VGA92_01135"
     CDS             complement(16383..17054)
                     /locus_tag="VGA92_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845049.1"
                     /translation="MKLSKLVLLISIIAAFVPAYGQEFSNTSLVLQKIDIPANEFNRV
                     LDDSTPIPFDTAHAGNWQINIENDLLYGNPKGSAIIRIYDANIADKFIEIGMGSPPDR
                     KFWVTIDDPERGLIPATRLDKDGWYEDSKIIAAYSDSQGISVGNGKRIVVSNVILDNF
                     VVGSYAVYGMNEPTDPPAINSGTFIIDIISGDVSQNPIHYYPYYVTGAVGAIIGVLLL
                     VKKRS"
     gene            complement(17057..18199)
                     /gene="sat"
                     /locus_tag="VGA92_01140"
     CDS             complement(17057..18199)
                     /gene="sat"
                     /locus_tag="VGA92_01140"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX9845050.1"
                     /translation="MTEHEGVIKPHGGRLINRIAQKNPDGLYSISISEDLANDVENIA
                     DGIFSPLEGFLLQADFESVIKKGRLANDLAWTVPIVLDVDKDTANKIKRAGDVALKNS
                     NGEFAILHIEDVYSFDKQKAAQAIYGTTDANHPGVAKTNSMNEYLVGGKIDYIRKPGE
                     DSIRKYRMTPQQTRKAFSDAGWKTIVAFQTRNPPHVAHEMLQKTSLTTHDGVFVNPLI
                     GKKKSGDFVDEVILESYLAMIDNYYPKNRCILATLHTEMRYAGPKEAIHHAIMRQNYG
                     CTHIIIGRDHAGVGKYYSPFAAQEIFSDYTDMAIRPIFFPAFYYCKKCLAFTNERACP
                     HSPEFHEQISGTKLRALIDEGQSPSEFIMRPEVIKVILSHKKPFVE"
     gene            complement(18196..18918)
                     /locus_tag="VGA92_01145"
     CDS             complement(18196..18918)
                     /locus_tag="VGA92_01145"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9845051.1"
                     /translation="MARFSQEEIDKINSKLKTPEDALRWAYDTFGDKIAKASSFGAED
                     SVVTDMITKINPKARFFTLDTGRLNQETYDIMDELAKKYNIKFEVMFPDANEVQEMVT
                     IKGINLFYESVENRKLCCEIRKVHPLNKILSTLDAWITGLRRDQNENRSMIEMVEVDS
                     LHGGIIKINPLIDWTWDQTLNYIKERKIPYNKLLDKGYTSIGCEPCTRAIKPGEDLRA
                     GRWWWESDTHKECGLHMDHSEQ"
     gene            19009..20127
                     /locus_tag="VGA92_01150"
     CDS             19009..20127
                     /locus_tag="VGA92_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein"
                     /protein_id="HEX9845052.1"
                     /translation="MNEAAFVVVFPSIFAKNKQNQLITNIKKILKIQNQHFGQLRKDD
                     GLIVIDANDPVFASSAINLLFGIDKITISRRTENRFDLLVSSIAKIGANLLLKGDQFH
                     IKVEGHSSGYLPKDVEVAATSALIEKTHHMDCRPGTEEKHDKQIYCFLTKKNAYISIF
                     LDSGHGGIPYNSQGEKIVCCIFDELSAVGCLESIKQGFDVRILVCYNDSNLLELVKMI
                     NRILPRTVSTQVTLDFFRLPLKQENAKSLQLKVRAVTEILCAAAKKEKIKRISLPLSP
                     LVFPIWFIDQNVSTILQNRLVPWIALAGVDNAIINTAKEIGLGKYLHKIERLGTLRFA
                     KNDSDVSGIVQNTLKTRQSITVDVGPNNTHDILDSLKH"
     gene            20159..21394
                     /locus_tag="VGA92_01155"
     CDS             20159..21394
                     /locus_tag="VGA92_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9845053.1"
                     /translation="MKRIGQFIYSWGNGHYSRMMSLNEELPNFVKDEYEIHYSSKDEI
                     YQKLLKQFPDKKQNIHEILMPTPIDGKYGPSIFLSMLNVLLPISGNPPVVRQISSYLR
                     KEAKLFDSIGFDLVINDGDMGPNILAYNRGVNCIFVTNQFMPKLWKSRFYFYPGVVFV
                     AKQIAKATKIVVADSPPPYTMCEYNLNFPEKLKEKIVYTGHFAADKKLRSAQKTDLER
                     LIEGNEFGYWMRTGNKSTNQITGKKYEEVFTSPEMKNEKRIISHATSDPPIDRVLGMD
                     GRTYSVCEALEKKIDWVQIDIGFLSEQQKDTALELCKYAVINGSHTVMGEIIGVKGKP
                     IIGIPVYDEQSNQIRWAQEHRLGIGANNKKQVITAILELKKNYAKFEESIQEFRKNFV
                     ANGAKTAARLAAQMLDDKR"
     gene            21541..21828
                     /locus_tag="VGA92_01160"
     CDS             21541..21828
                     /locus_tag="VGA92_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845054.1"
                     /translation="MVNCPECVAKERKAAFERQAASGSQEGLQKVIEEIEIPMKFDPV
                     TKHFICKRCGLYATREQVHDIKDRLNQRERTREDKHYDYLEWWQKSKKDKK"
     gene            21841..22236
                     /locus_tag="VGA92_01165"
     CDS             21841..22236
                     /locus_tag="VGA92_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845055.1"
                     /translation="MVKTKDELPDWVLDEIRNAKFAKSEPITRTGYILEVYDDDKKID
                     AQIYEAVEDGRHIVTLDLPKNIKPDDCQKGVVYEFNFDLLKVSLGKKVVDFLKNEKEI
                     EMPAIYQFALQKMELIDVGSDEAPSESEE"
     gene            complement(22239..23030)
                     /locus_tag="VGA92_01170"
     CDS             complement(22239..23030)
                     /locus_tag="VGA92_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9845056.1"
                     /translation="MSFKQKVVVVTGASSGIGAASCIEFAKKGASLVLVARRKNKLEE
                     MQKNLGKFGVKTLVCECDVSQKSQAQKMSKEVLDVFGRVDVLVNNAGFAIYGTVSELT
                     IEDIESQMATNYFGMVYCIKYFLPKMLEQNSGHIVNVASVAASFGLPGIASYCASKFA
                     MLGFSEGLRHELHGTKIGVTVVSPIMVKTNFFDHQSFEKMPKYSPTALSAQTVAKAVV
                     SAATSSRLEIIVPSIVRGAVWAKHTFPYIINPILGSAFRKQMAKK"
     gene            complement(23067..24077)
                     /locus_tag="VGA92_01175"
     CDS             complement(23067..24077)
                     /locus_tag="VGA92_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX9845057.1"
                     /translation="MNWRIVAFLASLVPFIIIAVTFKVKPEDIFAIGLINFLAAFAAM
                     MCKLFLQGIKFHYIIRKFHGSVETFWKTIFVRIGSEFVTMTTPMFVGGEVVRIYWMNK
                     RGLSAAKASWLGIFEIVTEVLAAGILAVSAGIVAIASGYTIIGVVVLATSIPIVSLWA
                     GLFFFSSKITFRVPSAVSKIIIKFGKERGTHYLEKTNQWMNEICTMSRENISSKQTRK
                     AFVVTFAISLASWAIFGLSFMLILAGSENAISAIESIFAVMAGNAIGNLPITVGGSGL
                     TELGVWAYLNHLSEFKLEMPKNSVEWNSIIAWRIATYQLPIPITWFLLMKMALRKYEK
                     LQ"
     gene            24311..25513
                     /locus_tag="VGA92_01180"
     CDS             24311..25513
                     /locus_tag="VGA92_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9845058.1"
                     /translation="MSIIRNYKFRLYPTIEQAKELQNNLDACRWVYNKFVESIQSEFV
                     SRNDLNYFLTELKQSEPWLYKYHAKMLQMVSTQLAGAQKALIKMHEIGRKPGNLEFTT
                     YNKHRTFTYNQFGYEITKHGNTDLLYLSKIGFVEIRLHRNFVGEIKQVTVTKSKSGKW
                     HACLVCDVDAELPKISLTKSVGIDVGIKNFAYDSDGFVTPNPLNLQKMLKPLARIQRK
                     ISRRQKGSNNRKKAVKFYQIIHERIKNRRRDFLHKLSTQYAKKYDVVFVERLQKLNMV
                     KNHRLARNILDSGWGTFANMLDYKTMLIDVPAKNTTIDCSRCGNMVPKSLAIRTHRCN
                     MCGLVLDRDHNAAINILKKGLDIFGITYQGSNLPQELREVTPVEITEWSRKQECSTAL
                     IGSKFTSQ"
     gene            25530..26288
                     /locus_tag="VGA92_01185"
     CDS             25530..26288
                     /locus_tag="VGA92_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX9845059.1"
                     /translation="MKVIFVAGTAGAGKSLLSSKIYQYYTRNGAFAAILNLDPGVISL
                     PYTPDVDIRDLVDIVSLMKQYDLGPNGALMMANDLIASKLDEIQQQADDINPDYLIID
                     TPGQIELFAYRASGPYFVNNFVAEQKTSLFLFDGVLMTTATNFVSMALLATSIKLRLG
                     LPTINVLTKIDLIADKISEILRWTTNIKTLENAISAEADGEAYVLLTNLLRSLNIGGF
                     AQGLIPISNASGDGLINLEAALSRILNLGEEVED"
     gene            26285..27202
                     /locus_tag="VGA92_01190"
     CDS             26285..27202
                     /locus_tag="VGA92_01190"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9845060.1"
                     /translation="MMASITVRAPSSTANLGPGFDVFGLALDAFYDEVTLTKKKDGIT
                     IITFDNIPTSAQKNTAGLVVQYMKKRFKVKSGVQIKIEKGVPAGFGMGSSAASAAATA
                     VAFDRLFQLGLDDNSLVECAGVGEKASAGSIHYDNVSASVLGGFVIVRTNPLDVINIL
                     PPKDLLLCVAIPKLDVPPKKTAVSRSVVPKNVKLKDSVTNLSNAAAIVAGFVRGDPEL
                     IGRSIKDVIVEPARQHLIPGFSAVKKNALRAGALGVTISGAGPSVIAFATKKSDLKKI
                     AKAMKNGFASAKTDCNVVICKPTNGARIK"
     gene            27220..27894
                     /locus_tag="VGA92_01195"
     CDS             27220..27894
                     /locus_tag="VGA92_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX9845061.1"
                     /translation="MKKKAVVILSGGLDSLCLGAHLRSKYDLYGITFSYGQRASKELN
                     AARHVGKILKLKEHKVVRIEFMKSLYGQSNVLTSTKKKIPSKFDYSIVVPIRNAIFLS
                     VATAWAFSLRASLVAYGAHTDDKKYPDCRPAFSKKLESALNEGEIDGIRLGLRNKIQI
                     WTPYLAGLSKSTLIKTGYKKFGNNIFKTWSCYSNTALHCGRCESCNNRKLAFLQAKIK
                     DKTKYL"
     gene            complement(27891..28145)
                     /locus_tag="VGA92_01200"
     CDS             complement(27891..28145)
                     /locus_tag="VGA92_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845062.1"
                     /translation="MGVHSNVHIPKESWPYWTWFAIEFFIVMAVAALSSREVVRMFEQ
                     TFDPAMQNWVFFGIIGAIYLAWYIGIRPLVFKRKILETRY"
     gene            complement(28181..28735)
                     /gene="folE"
                     /locus_tag="VGA92_01205"
     CDS             complement(28181..28735)
                     /gene="folE"
                     /locus_tag="VGA92_01205"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9845063.1"
                     /translation="MNKERVKKLVRELIIEIGEDPTREGLRGTPDRIADMYEEIFSGY
                     DSDSELSVQFCEDSDAVVIRDINFYSMCEHHMLPFFGKVSIAYLPNGRVFGLSKLVRL
                     VEKHAKRLQIQERMTKDIADELYSQGVKGAIVLSDAEHLCMKMRGVKNNATMTSAAYR
                     GVYERRENREDIVAMIRNSKPSVF"
     gene            complement(28735..29448)
                     /locus_tag="VGA92_01210"
     CDS             complement(28735..29448)
                     /locus_tag="VGA92_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX9845064.1"
                     /translation="MKLRLYEIFTSIEGEGILYGTKTLFVRLAGCPFTCFYCDTKEAL
                     PLDSGQEYELADACALIDKHLQSQTYKVNFTGGDPLIQHEAVAELAKHVHSKNIPTYL
                     ESSCFDSKKFSQVLPHIDYVKIEFKTQDSEFVDNKHYSTLLENALECLKSAIVSKKST
                     YIKIVVSAKTEPKPFEDLVSKIFKIANPKDLKGFIIQPTYGIAEPNLQQLFGFYDIVY
                     PYYNEVRVVPQLHKFIGAP"
     gene            complement(29484..29678)
                     /locus_tag="VGA92_01215"
     CDS             complement(29484..29678)
                     /locus_tag="VGA92_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845065.1"
                     /translation="MASRQIYGGVGIPMIVVGALMAFLWAPTNTEFIETIEFVGALIG
                     ILGVVFFFAGLLYAKEPVVS"
     gene            complement(29717..30949)
                     /locus_tag="VGA92_01220"
     CDS             complement(29717..30949)
                     /locus_tag="VGA92_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845066.1"
                     /translation="MKQILTFTLIALVASMIVGLPTTFANSTLDSLVNIATKARTQVQ
                     FQLERTANVSDELKALFERGSQETELLISAVKQNDVAQAKQHFLAAMKIFKQVSVELS
                     EPQQAVTTLKTSQVDPAILTRYQNDIDRTEKYVDMLKDLVTKNDFTVDFLKVEELIQN
                     ARSSLAASDIPSIERIVGQLRVALNDVQNAISEQTRERQNERIRSFVNDYIATTETKL
                     ADFEPYMDETIKPKFEAVRQTIAELKNQTSADEATKLLRHLDSLVKEIEDYAKAKRDQ
                     MATQQAQAEQAKSEERPAEPKQERSTQDDTKNQKVSSEVLRLEARLANIKPYVDENIQ
                     FKFETSERLLTKLTSQGTSGYADYLRTVRILDSLVDSLERYVESLQNNDDDRSDIADK
                     NQPNKSSEKEKSSTERNN"
     gene            complement(30946..32151)
                     /locus_tag="VGA92_01225"
     CDS             complement(30946..32151)
                     /locus_tag="VGA92_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845067.1"
                     /translation="MKTQEAVLCSIHDIPDIEGMLFVRITDILIRMLKAPLLAIGIIL
                     AVLLPTQHTFGLLRTAEFTIYPDGTTHVSQEASADPQNPELSIPLFGSVIDNFVAQDE
                     NGLLLLYEIGENQVVIQTFGATNVSIDYDTFDLVSKQGKIWTFEIDSPVDYTMTMPED
                     TVIVGMTNFPNLVETINNKPHLSLPKGENQIDYFFGVSGPAQSASNNIEKSKSLIEQI
                     NDIGIKTPQATAKLEEAVLSFESRQYADAEKLALEAQQIALQEQQTAKNNTVLPGDIS
                     TLSDNIIGIAASIAVIGGAITTITLVFKRIKSAVKKKIEPIMAREESEIESEETTQQM
                     REDDKQLVAFLEQNGGQAFEKELRKKFLLPRTTMWRAVKRLERQGVIVIEKKDFQNLV
                     KLRKGEGEQ"
     gene            complement(32154..32441)
                     /locus_tag="VGA92_01230"
     CDS             complement(32154..32441)
                     /locus_tag="VGA92_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9845068.1"
                     /translation="MSKQQYRSEMGIMGDILNVTMEGGRQGTIVSAISRRANLSHYAV
                     IEKCEKLSTAGLVQSVRTDKNRLYMITEKGIEFVQEFRKFQSLLDSLNLRY"
     gene            32673..33167
                     /locus_tag="VGA92_01235"
     CDS             32673..33167
                     /locus_tag="VGA92_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9845069.1"
                     /translation="MFERFKRGDTSHEDAGVMEQERPDTNSENVAEQVRIPEPPSSSG
                     SDIGISELMSKRAKLEEAIDYVGLMIKNLKDKRTKLEKEIEDESVDIKNLKEKLAKVG
                     EYIDEENRGIRDLSNKRAQVEREADEVGSLINNLRSKLANIDSIIDGEANKVKNIKDS
                     RSKA"
     gene            complement(33243..33884)
                     /locus_tag="VGA92_01240"
     CDS             complement(33243..33884)
                     /locus_tag="VGA92_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Snf7 family protein"
                     /protein_id="HEX9845070.1"
                     /translation="MTHFQKSWSKPPTPSISEKINDVIKPKGALKPRIEQAVKKLQSQ
                     ISKLDGMLTKLKQREEKLFQRIVAATQKHDIHTSKVLANELAEVRKVSKMLGNVRTAL
                     EQIELRLTTFHDLGDTVVTIAPTIGLMRSLKGSLGKFMPEADRELGMMTEMLNGLMME
                     SFSGESAFGMDQSLNEESEKILEEAAAVAESSVDAKFPSMPVSSQSATSSRYL"
     gene            34600..35511
                     /gene="tfb"
                     /locus_tag="VGA92_01245"
     CDS             34600..35511
                     /gene="tfb"
                     /locus_tag="VGA92_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483118.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HEX9845071.1"
                     /translation="MTQTTRVSDRCPRCAKGSMLTDGSTGEMFCNTCGFVATERVEEE
                     GPEWRSFSKEEGENRTRTGTPTSLAMHDMGLATIIGQADKDASGKPLTSSMKSTIERL
                     RTWDSRSQVHEPVDRNFRQAFSELDRLKDKLALSDAVIEKTAYIYRKALDKGLVRGRS
                     IPGLVAAALYAACRNTETPRTLTDVANGINIKRKDVARCYRLLLRELDLKMPVVDPIK
                     GVARIASIADLSEKTKRKALEFLKEASRIEVSAGKDPMGLAAAALYLACVMLGENKTQ
                     KDIAQAAGVTEVTIRNRYKGLKEALKL"
     gene            35567..35998
                     /locus_tag="VGA92_01250"
     CDS             35567..35998
                     /locus_tag="VGA92_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845072.1"
                     /translation="MNKRTVSGAIVVGIVTILAALVLMPSEKSERQEIYHARLADPKL
                     YTGGIFTDTFAIEKGTYQFRFVPNGDSPQILSIALKGDAFSFSEDFELEGTPHETGVS
                     TYYTWEYLGQDTIEVSEDQELQIVIDPHGNTLGPVSVYLIK"
     gene            36264..37007
                     /locus_tag="VGA92_01255"
     CDS             36264..37007
                     /locus_tag="VGA92_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9845073.1"
                     /translation="MKLEGKVALITGGSRGIGKAIANLFAKEGASVVISAKDPTSLQK
                     TAKELGDVFAVPADIREESEVQNVVNKIIEKFGRLDILVNNAGILPKIKPLHEITEDE
                     WNEVIDINLNGQFRFTKAAIAHLQKNGGCIINIASTAGLKSYENFSADAYSASKAALV
                     LLTKCWSLEYAKSKVRVNCICPGVIETDMTKEFLATPALREMLNFEYPIGRIGTVDDV
                     AKSALYFASDDSSWVTGAVLALDGGESAK"
     gene            37052..37261
                     /locus_tag="VGA92_01260"
     CDS             37052..37261
                     /locus_tag="VGA92_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845074.1"
                     /translation="MDSKKKTRAKTIILLAIIWFVVSLPLPWFFTAPDEVKPQVFVLL
                     QIVGIISIPFIMLAIVWTIKPELTT"
     gene            37263..38081
                     /locus_tag="VGA92_01265"
     CDS             37263..38081
                     /locus_tag="VGA92_01265"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9845075.1"
                     /translation="MSVKIPFTNPLDEAALAVDAAIKASKAVMDIYNTTFRTELKDDD
                     SPITQADLESNEIIKEVLSSSGLPILSEEDEDDVGRLSHKKIWIIDPLDGTSDFVNRT
                     GEFTIMIGLVENKKPILGVICRPTTNTIFLAQKGNGAHKLENNSWQKLTVSKTLDLRK
                     CKAVGSRFHLSDQEKQFFKNLGVSSFESRGSSLKVAEICMGLADLYLTTSNKIKQWDT
                     CASYCLITEAGGKMTSMNGAEILYNTEKINHENGLLVTNGLVHDEIIRRYLSVN"
     gene            complement(38070..38585)
                     /gene="cysC"
                     /locus_tag="VGA92_01270"
     CDS             complement(38070..38585)
                     /gene="cysC"
                     /locus_tag="VGA92_01270"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX9845076.1"
                     /translation="MAFVVWMTGLPCSGKTTIVRAMQKHVPNLAILDGDELREWLSPK
                     DFSKEGRIEHNKRVAHLAKLLLKHNVPCGVSLVSPFIVNREDARKIIGDDKRFFEVYV
                     KCSLQECETRDVKGMYKKARNNEIKAFTGISDPYEAPENPDLILDTEKETLDQSVQNL
                     LNFLKSKNLVD"
     gene            complement(38622..39164)
                     /locus_tag="VGA92_01275"
     CDS             complement(38622..39164)
                     /locus_tag="VGA92_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9845077.1"
                     /translation="MSATEVLRQDHIKIKRLEKIIEKCYADLYAGKNIPLPDIQKITF
                     VISEFLDSIHYSREEDSYFACVASYGTLKDEIRKFMIEHEFSRGIAKNIVKHLQQWQN
                     GEDAREPVARFLRTYHIYLNDHLQKEEEFFKKAQGEVLSKEEEQEMYEQFQSVMAVST
                     KIQTIIKEIEYLEKQDWVTS"
     gene            complement(39309..39950)
                     /locus_tag="VGA92_01280"
     CDS             complement(39309..39950)
                     /locus_tag="VGA92_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9845078.1"
                     /translation="MKAIVLGGSKGIGKAIADALSSINCDVIATSRKEIDTSNLSSVK
                     KFIAKHKQTDILVLNTGGPPPKQFRDVTEKEWQKYHNQLFLGFCLLLQKLKVRDSGYI
                     FVITSSVIREPNPRLVISSAYRLAFTAVFKLASKDLAARKISCINIAPGLINTDRLRD
                     LGNADDLAKTVPMRRLGEPKEIGNFVKAIIQNKVKYLSGVTINFDGANSNFVL"
     gene            complement(39991..40818)
                     /locus_tag="VGA92_01285"
     CDS             complement(39991..40818)
                     /locus_tag="VGA92_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9845079.1"
                     /translation="MDLVFSDIHADQDGLDTILDIVFSDEFQNQYGTVSRIINLGDVL
                     ERGTTPKEVLQKMTELSKSYPMISVMGNHDEGFLYKRFLNGSSYASLKAHELLSEQDL
                     EFFKQNKDGTYGNQFVIDQKNRFLIVHGGPITPEKVIQEGDDPWLYQRTWQRLSEEDN
                     EFYSYYGYHYKPASAFAEGKTHFDNFIILCGHQHIEAAIKQDKNQITNIWSFQHETQK
                     IGAHTLRKHEFPIEQNHNYLFRFGLAGPQGHHSGGFAKPHFGIIQDDPRNVILFGLE"
     gene            complement(40893..41054)
                     /locus_tag="VGA92_01290"
     CDS             complement(40893..41054)
                     /locus_tag="VGA92_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845080.1"
                     /translation="MSKSNQTLVSVYTSMLKTLIDRRAELIKEIEDINNQIIELKQEA
                     NESGVALAS"
     gene            41255..41584
                     /locus_tag="VGA92_01295"
     CDS             41255..41584
                     /locus_tag="VGA92_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845081.1"
                     /translation="MFEDLVREAIREPTEAAASSVMQEGIPSEVRGFIPDLVRQLDAG
                     VPSDVVADQALNKTFDIVESVVLTNATSHVLDKFSQRIVDLLFGEPFLNILAQAAALI
                     TNSTSPV"
     gene            42016..42888
                     /locus_tag="VGA92_01300"
     CDS             42016..42888
                     /locus_tag="VGA92_01300"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9845082.1"
                     /translation="MSITVIGGKVSEGLAKRLAEKLKADFVSAELRVFPDGESKITIS
                     KKPRKGKIIVVNSTCPPVDSNLIQTLSLISKARQFSGQVICVMPYIGYARQDREFLPG
                     EVVTLSVIAKLLQSAGASKIIVVDIHSRLGLKHFKIPIKNVSAVSELVKYFKKLKLKD
                     PLVVSPDLGGMIRAKEFARMYGTDFVALKKQRDKKTGKVRIMSSSLDGVRGRDLVLVD
                     DMVSTGGSIIKATEFLKRQKCGRVFVACTHGLLIDNAEKKIRKSGVSQIVSTNTIPGN
                     TSVVDVSGIIAKAI"
     gene            42889..43836
                     /locus_tag="VGA92_01305"
     CDS             42889..43836
                     /locus_tag="VGA92_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX9845083.1"
                     /translation="MPESFFIVSKEYLELAVDEIVTLVKMYDRFSKINVISNLIIIQT
                     KTPWEKIARRATFVKTSGQLIRKMSNVFFDEKNYALLFGAKSFMCKAINLSQKHIDIS
                     EIERSLGSMVTTFCNAKVSLENPDMVIYLIFTDEENFFGFSTRFEASRRPKKPTKFHH
                     ELDWKLTRAMINLARLNDDETVCDPFCGTGSTLLEAESMGIRSIGIDFDKKMCEISKK
                     NLRFNGFDAQVFNQNYDYMLQIKDKFDGIVTDLPYGTASKVSEPPQKLISNFVSKIPK
                     QAKFAIMCKKGLDKDIKLKPTKKYEIYRHKSLTRVILVK"
     gene            43833..44732
                     /gene="rnz"
                     /locus_tag="VGA92_01310"
     CDS             43833..44732
                     /gene="rnz"
                     /locus_tag="VGA92_01310"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEX9845084.1"
                     /translation="MKLVFLGTSAAQPTEARGTSCICLEKDGEVLMFDAGEGAQVSYL
                     RSGLGWNKKMKIFVTHLHGDHCIGVLGLLQTMTLQHRTEPIEIYGPDGIDEFIAQNIK
                     ILNFGLSFPVMITTIKPGLVCEEKTYLVFAEHAEHSVVAFSYLFQEKDRPGKFDLQKA
                     KSLGIPEGPLWHDLQMGKEIKVGNKIVKPSEVLGEKRPGKKIGISGDTRPTEILEGFF
                     NNCDYLSFDCTFSDKLKDKAVETYHSTAKEAAFFAKKANVANLILTHFSARYKDESEL
                     LQEAKEIHGSVIAAKDLLEIEIK"
     gene            44734..45456
                     /locus_tag="VGA92_01315"
     CDS             44734..45456
                     /locus_tag="VGA92_01315"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX9845085.1"
                     /translation="MFDLVAKQIKGAKKIVFVTGAGISQESGIPTFRGKDGYWKKYDP
                     MQLATIDAFYQNPKLVWEWYEERRKNIFAAQPNKGHLAIAELAAHKDVIVLTQNIDGL
                     HQKAGSKKVFELHGSIIRIKCTTCDHIDNVTTSFDFLPPKCKCGNMLRPDVVWFGEPL
                     PQDVWINAIMHAQSCDVMVIAGTSLVVSPANTLPLYAKQNGAVLIEVNPERTLMSEDV
                     NLSLRETSANALPHLVSLFDRT"
     gene            complement(45465..46265)
                     /locus_tag="VGA92_01320"
     CDS             complement(45465..46265)
                     /locus_tag="VGA92_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845086.1"
                     /translation="MILKIAIVGVILVIGGIMLYPEIGKLSSTHTSGLDAVAQDLGDL
                     KDTTVQRVNYEFDKTVDSVGDKIEQIAPDPDEINPIKKIQEKITVPQPQKEVYYGEVY
                     EKDDENNCKISVPKMVKTVNGVKELTHTITLPDCQYEKRQSVQVTVTNPQTNTQTISV
                     DPTPQTQIFETLQLTTMRNDDNTVSIHYEDTSGKTLKVTVTLRNSEKELFSGEFFASQ
                     FDTSVNDVSTSPHIVEMIVEHADYGTVSSSVFNPQGSDDATIYGVFTQ"
     gene            complement(46340..46744)
                     /locus_tag="VGA92_01325"
     CDS             complement(46340..46744)
                     /locus_tag="VGA92_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX9845087.1"
                     /translation="MAQMIPVVDCKIEAFCEINPSEDPQKLEQVISNVFDNIEIKTTN
                     DTIKANSKDISSLSKIHESIQNHKSQRVYFRFLNNNLEENSTWFYLNKQAAFANFISL
                     CEHDNESPLGPIKVVIHSKNIERIIEWLTGQN"
     gene            complement(46732..47280)
                     /locus_tag="VGA92_01330"
     CDS             complement(46732..47280)
                     /locus_tag="VGA92_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845088.1"
                     /translation="MPKLIVCLTGMPGAGKSTIAAGLQKKGFTSINMGDAVRAEAKKR
                     NLAPTGENLGKLMLDLRQKNGQGAVAELIKDQIINSESDVVVIDGVRSNTEIEVLKKI
                     ATVKLLSIHASTNTRYNFLTTRKRTDDPQDRNTFDERDNREISVGISASIALADETIS
                     NNNLTIDQLIDTAYEIIQKWLK"
     gene            47385..47924
                     /gene="thpR"
                     /locus_tag="VGA92_01335"
     CDS             47385..47924
                     /gene="thpR"
                     /locus_tag="VGA92_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9845089.1"
                     /translation="MRTFVAVEISNQQILDSIKRLQSSLKIEAKPVDTQNMHFTLMFL
                     GEVSDEMAQKIGAQLKTIEFSQFNMSFEGIGAFPKPKFPRVVWVGLDKEGGSMLSSLA
                     KKVEEKLAPLGFSSDKPFKPHATIFRIKNKVEDISDELVKYSSTKLGIQKVSELKFKK
                     SVLTPSGPVYSDLEVILAK"
     gene            47921..49255
                     /gene="cca"
                     /locus_tag="VGA92_01340"
     CDS             47921..49255
                     /gene="cca"
                     /locus_tag="VGA92_01340"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9845090.1"
                     /translation="MIEILKRAEELVTPSEKLHKQKDSIVEQVIRLVQKQISSYPQIT
                     GIEIVGSFAKGTWLPDKADIDIFIKFQTSVSEKEFSELGKKIGFATLKDYNPYVRYAQ
                     HPFVEAEIKGTKVNLVPCYDVQEGGWKSAADRSPFHTRFMINSLTAQMKNEVRLLKAF
                     LRANDIYGAEIARQGLSGYVAEVLVLNFGSFAGVIKAISELKQGQIIGKAAKEFDTAV
                     VIMDPIDSNRNLGAAISTESVGKFVLASRAFLKKPSISFFKPKKKPQRKPNDLRKNVL
                     VLSFRYKQRSPDIIWGQLKRAATSIATQIEEEGFSVLRKTAIISEDANAVLLFLLQST
                     KLEQNLLRVGPDFYTVEHVEKFISSNKKKSVTMWLNEQGKICSIQKRPQTDVMLFLKD
                     LIRKNLDRVGVPAGLKDDIKAFKLVLGHKVTSKSIKEAISELVSTDETIFSS"
     gene            49233..49988
                     /locus_tag="VGA92_01345"
     CDS             49233..49988
                     /locus_tag="VGA92_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX9845091.1"
                     /translation="MRQFFPVDILAKEPYSIVLGYPKASRRQLKSRLAELKKLGIEDV
                     SFQGPILIGKIAVLGKGYTGVVILAKKGAKRVALKIRRIDSPRESMAKETILLEAANK
                     AKVGPRLIESSDNFVVMEYLDGKKIYDWINELKGRGRATRLKSVIKKVLEDCHSLDRA
                     GLDHGELSNITKHVIVGKSVTIIDFESSSLERRVSNVTSATQAVLIGSGLAKMVRRIY
                     SAPSKQKIIKSLRQYKEDQTQESFDKVLEVLRL"
     gene            49990..50595
                     /locus_tag="VGA92_01350"
     CDS             49990..50595
                     /locus_tag="VGA92_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9845092.1"
                     /translation="MSKQAVKFLKKDPKLAKIIKYVGSYEISLMKNPYESLVEAIITQ
                     QLSGKAAESISKRFRKLFVRFPKPADVIKMPDSKIRLAGLSGMKVKYIKDLSEKIESK
                     ELKMRSLKNMTDEEVIMQLTQVKGVGRWTAEMFLIFSLGRLDVLPVGDLGLKKGIQRM
                     YSMPDLPEKDEIEDLAEKWRPYRTVATWYIWKSLNQFDKIG"
     gene            50643..51626
                     /locus_tag="VGA92_01355"
     CDS             50643..51626
                     /locus_tag="VGA92_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845093.1"
                     /translation="MQNTLVTVFSCLVVISIVSFVLQDQFANAQEIRIPNWIKSQIKS
                     WADRQASDGDFVELLGELNKQGLIKTHDMNKNQNMYLLPKYGQTIFVKIAGRTGDFEQ
                     TSPVTLIVVDPQGQRSEYTILVLESSVYSTVVPISHDSPIGTYTVFAYHTNKELPTSY
                     FFVKADPQIPWWIKDVAKWLAADKISDRDFILSMQYLIDKKIIDLGFIASTNQDSFLD
                     VSVDGLKAVRRGTMQSLDVHVSNMAGPVDGAMVFVRVEDYGENILEEFTGVTDSSGNY
                     NLSWELSMDEFNDIKTLLVYVDVTNGVSSKTKLFTFEVYCLCGEPNCKCRN"
     gene            complement(51639..52418)
                     /locus_tag="VGA92_01360"
     CDS             complement(51639..52418)
                     /locus_tag="VGA92_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9845094.1"
                     /translation="MGKNETEVVPEKLDSPINLKIIMLIVVSVTVFHYASIYVLQGDI
                     SDLASYAFSILNPIITTAAGFIIAIRYRSTKIFFKSYLFLAIAYFCAAGGETLYFLYD
                     YVLEEPAFPSPADILFIPFYPLVLGHLFLNVSFFKPKIRAKTLLWMMILPISLVLFYL
                     NLVWGFERDGLFLLSLYYIIVSSVSLTMTIYGASIFREGIMGKAWLLLLFGVIAFSVA
                     DVIYYNLEANAGYTLEHPVNLLWYAGYWIVTYALYKHRKIL"
     gene            complement(52463..52536)
                     /locus_tag="VGA92_01365"
     tRNA            complement(52463..52536)
                     /locus_tag="VGA92_01365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52500..52502),aa:Pro,seq:ggg)
     gene            52590..52799
                     /locus_tag="VGA92_01370"
     CDS             52590..52799
                     /locus_tag="VGA92_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845095.1"
                     /translation="MKICSICHKISATDQDHTDCKEKLRVELEAEDFKKGIPEKLDMV
                     KNANDIGPDIKALLGHISREKKKES"
     gene            complement(52794..53933)
                     /locus_tag="VGA92_01375"
     CDS             complement(52794..53933)
                     /locus_tag="VGA92_01375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX9845096.1"
                     /translation="MQKQILILGGGFGGLAAANELRSNLSSDVRITIIDKKDYFMMDL
                     VKLWIIKGTRQFETSKKPLQSITKKGIEFVNEPVTVIDPQQRIVKTTTKEFSYDYLII
                     AVGVELAPEKISNLATYGTILYDIEHVPKIRQRILDMKSGRLVFAITGMPYKCPPAPF
                     EAALIINAMLKELGKRNAITIDFYSLAPITLPAAGPEVSKQLLEMLESENIQFHGSCK
                     TISVEQNTLKFENGNFTKFDLLIAVPPHKAPKVVYDAGFAQEGQFISVKRDCKTQHDG
                     VYAIGDVTNMLVTSNTAVPKAGIFAEGEGVAVAHDIISQIKKEKTDSIFDGKGGCFVE
                     VGETAGYVYVDMFAEPNPITRLDKPAEEHMLEKEKFERERLQKWL"
     gene            complement(53935..54675)
                     /locus_tag="VGA92_01380"
     CDS             complement(53935..54675)
                     /locus_tag="VGA92_01380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9845097.1"
                     /translation="MIKDKVAIITGASSGIGKATAIALSNAGAKIAIGARRSDRLEEL
                     ESQIAKNGGDVFAQKLDVTKKSDCDSFVDTVVKKWGTVDILINNAGLMPLSFFKNLKV
                     SEWDQMIDVNIKGVLYCTAATIPHMLAKKSGHIVNISSVAGRIVFPAGSVYCATKHAI
                     VALSEGLRQEFSQRSNIRVTCVEPGVVATELTNTITDDSLKAFVESAKKMEALKAEDI
                     ANAIVYAVESPPHVNVNEILIRPTTQER"
     gene            54794..55012
                     /locus_tag="VGA92_01385"
     CDS             54794..55012
                     /locus_tag="VGA92_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger domain-containing protein"
                     /protein_id="HEX9845098.1"
                     /translation="MKIVNCTYCGDETDLPFECNYCKDQFCAEHRLPEEHRCVKLTSI
                     RAKRFGEKKVIRRQKRGFLDKVFGKHKD"
     gene            complement(55009..55284)
                     /locus_tag="VGA92_01390"
     CDS             complement(55009..55284)
                     /locus_tag="VGA92_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845099.1"
                     /translation="MSHLDVDVIDFLLLTIYPVVGLFVIEIISRVVKLANWIKLVSQG
                     IVSVGFGIAYVTLITAHWLTALVLFALAVALFYQARISKLTPGKSIY"
     gene            complement(55313..55870)
                     /locus_tag="VGA92_01395"
     CDS             complement(55313..55870)
                     /locus_tag="VGA92_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845100.1"
                     /translation="MSETKKIDVNSLYSVLLREIENDQVQEIDPEFYKTLSEYLGKLK
                     SEGYDGLENKIKNRLVEMITQITFLLLKTRIEKVTTQDDLDYTNLLDEEKFIVESQQQ
                     LQERKEMILSATLNGRTKLLESVSKKHKTKPITVRFLRDFDQFVGSDFTRYGPFKAED
                     VATIPNENAQALIAKNISIKLHLED"
     gene            55927..57387
                     /gene="glnA"
                     /locus_tag="VGA92_01400"
     CDS             55927..57387
                     /gene="glnA"
                     /locus_tag="VGA92_01400"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9845101.1"
                     /translation="MPYKVTHGEATQTKYSADEVFAKIKHENIRFIDLQFTSLVGRYH
                     HTTISAALFTPDQMKDGLPKLDGSSIVGFTNVDDSDLILKPDPNTFAIIPWMTEHKTA
                     RLICDIYWGRGRGRLERDPRGIAQRAEEHLKTQGFDFSYWGPEVEFFVFDKIVWDVLT
                     PYKGQSYSIESKEAPWSQEGTGYPMGLQEGYYPSTPSDTLTAFRNECVDVLNENFGIL
                     CDNHHHEVATAGQCEIDIRFDYLTNAADGAQSYKYVVRNTAKKHGKVATMMPKPISMD
                     AGSGMHCNVSLWKDAKNAFFDKDAKDEISQVGRYFCGGMMTHARALCAITNPTTNSYH
                     RLVPGYEAPVYIAWSPSNRSAAVRVPEHFRGEKYAYLKRFEYRVPDPSSNPYLVFPAL
                     LAAGLDGIKKKMDPGDPVLEDIYKMTKEERAKRGIKNVPANLGEALDELESDRKFLNP
                     IFSNDVIDKIIELERKDHREISIRPHPHEFYLYFDV"
     gene            complement(57384..57665)
                     /locus_tag="VGA92_01405"
     CDS             complement(57384..57665)
                     /locus_tag="VGA92_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845102.1"
                     /translation="MAGITKVITSVKYAYLAAFFALLSGVFYPFIQGGTLDYMFLGIF
                     ILVVGLAGTVLVYKAATSNKRRGIYLGIGFGLTAISLFYILQLTGRPLA"
     gene            complement(57721..59424)
                     /gene="thsB"
                     /locus_tag="VGA92_01410"
     CDS             complement(57721..59424)
                     /gene="thsB"
                     /locus_tag="VGA92_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX9845103.1"
                     /translation="MSLQAARGGSLPVVILKEGSSENKGREAQKNNIMAAKIIAELVH
                     TSLGPRGMDKMLVDSLGDVTITNDGATILKEIDVQHPAAKMLVEISKTTDNEVGDGTT
                     SAVVLAGALLENAESLLTQDVHPTIIVDGYRKASKKAIQYLKEISEDVTPNDKSVLNK
                     IASTSMETKLVRKGGELLATLVVKAVLAVAERNGEKFIVDQDDVKVEKKAGGSIKDST
                     LIEGIVLDKEVVHGGMPKKIAEAKIALINTALEIDKTEFDAKINISNPQQMKTFLDEE
                     NRMLKNMVDKVIASGANVLLCQKGIDDMAQHYLARAGILAVRRVKESDMAKLAKATGA
                     RIVTNLDDLFEKDLGAAELVEERKIEEDRWVFIEGCKNPKSVTLLLRGGSQRVVDEVE
                     RSVHDAIMVVKDVMERPAIVAGGGAPETYAATKIRTWAKSLEGREQLAAEKFADSLEV
                     IPLTLAESAGMDPIDTLTNLRSRQLKGEKWTGIDVMKAKISNMKTSDIVEPLAVKNQI
                     VSAATEAACMILRIDDVIAVAKSASPGGPEGGMPGMGGMPGMGGMGGMGGMGDMGMGE
                     M"
     gene            59626..60948
                     /gene="glyA"
                     /locus_tag="VGA92_01415"
     CDS             59626..60948
                     /gene="glyA"
                     /locus_tag="VGA92_01415"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX9845104.1"
                     /translation="MTKSTYKTAYKKILADLQEHSRWFENSIPLIASENVPSPAVREA
                     LTSDFGNRYAEGWPGERVYAGCIFIDKVEIQCMDLAKKLYRAEFADVRPISGVVANLV
                     VYSAFTNPGDVMIAPSIPAGGHISHGKKEHSGTAGLVHGLDVEFYPFDADEMTIDVDK
                     TKEKIEELKKANRLPKMAMFGGSLFLFPHPVKELADFLKAYNIHINYDAAHVAGLIAG
                     GQFQDPLREGADTMTMSTHKTLFGPQGGLVLSYEKFGEAIKKATFPGLTSSHHIHHMA
                     AKAITFAEALEFGKDYAKQVIKNAKALAVALSENGFKVLGEKRGFTKSHQIAVNVLDY
                     SDGGKVEAELEKANIIVNRQLIPGDIKAGRNYFHPGGIRLGVAEITRLGMKESEMKEI
                     AAFIKKIVIDKKDPKKMTPKVKAFRKDYQKVRYCFDNKLGAYEYVKLR"
     gene            complement(60949..64326)
                     /locus_tag="VGA92_01420"
     CDS             complement(60949..64326)
                     /locus_tag="VGA92_01420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HEX9845105.1"
                     /translation="MSENAAISRIKGVQMPNYYLDYYSQISEKTYQTFEKAAEAKSTL
                     TDCSGNVEPKIAFDLADRVAKMHDIDIAEPLRALLKTKGKELAALDLSESIALGQYLP
                     PETPLEQRLDHAVRVGLAIVTEGVTIAPLQGISAVTIKKNRDGSEYLSVSIAGPMRSA
                     GGTESAVTMLIADHVRKVVGLGKYEANAFDDETGRFVEELRVYERDVGNFQYHVLDED
                     VVTVISNLPVELDGVDTDPNEVVNHKNMARIKTDRVRGGALRVLNDGIIGRAKKLLKR
                     IELYGLDGWEWLSELKGGIQKGDEADSAARRLKEVIAGRSVLSLPNKIGGFRLRYGRS
                     CNTGFAAVGIHPVVAEILNHTIAVGTQVKLDVPGKGATIAFVDSIETPTVRLKNGNVV
                     KIQNVEHGIKIKDQIEKILYLGDILISFGDFLENNAQLIPTGYVEEFWIEELKSKILK
                     YEPNSQELQQFLIRTPNFEEALELSLNFQIPLHPKYLFYWEQISVMELRQLLQPIKIQ
                     DDTISYSLNCKKILENLGVPHEVVSDELILQETEAQIFYQLLFANQIKLDDADTVPQI
                     ITKSSAIIIRPKFSTSIGVRIGRPEKAAPRLMKPPVHTLFPVGDKGGSTRDLLKASSS
                     PNFYCNIYNRICKNCNEPSISINCQKCGNKTTIEFVCTICRASLEQPFCQKCKKKVPT
                     HSFKPFPLKEKILAIQEKTGIRVQEPFKGVMELISQDRIAEPLEKGLIRQSLDLTVFK
                     DGTIRFDATNSPLTHFKPSWIGTTVERLYELGYTEDIDGKPLTNPDQIVEIKMQDVVI
                     PYESGRYLVNVCKYIDTELEKLYGKMPFYNVRNTDELIGQLVIGLAPHTSVGIVGRII
                     GYTNTHVCFATPNWHSAKRRDADGDADSIMLLMDALLNFSRQFLSDKIGGLMDAPLLV
                     QPTVLPHESQPQAHNLEVTKILPLEFFEDTLLGRKATEVTSVEIIKSRLETEAQFYGY
                     YFTHITTTLTTSRSRSAYSTLGSMLDKFDMQIRTAELINAVDTNEIVTNVITTHLVPD
                     IIGNLRAYGKQKFRCTSCGRKYRRTPLLKHCICGNDLIQTITRPSIEKYLHLAKRLVE
                     KYEVGPYVKGRIATLADEIDLVFGKRKGDQLLLTDYQ"
     gene            complement(64328..65080)
                     /locus_tag="VGA92_01425"
     CDS             complement(64328..65080)
                     /locus_tag="VGA92_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEX9845106.1"
                     /translation="MAKIEEYLRVTRKKKSALIFILIDSEASKIKESVRLAKSVERIG
                     ASAILVGGSSAIDQLEMAQVVKTLKKSIKIPIILFPGNVTGVVPNADAILFSSLLNSE
                     NPYYITQAQALGAPSVLKFGLEPLPTAYIIIGEGTTAWFVGSARSIPFDKPNIAAAYA
                     LAAQFLGMRFVYLEAGSGAKQNVRPDMVRTVRKVFKGFLIVGGGIRDAKTAKELVRAG
                     ADALVIGTMLEKEKDSLRKLTEIANSIKSNKK"
     gene            65146..65508
                     /locus_tag="VGA92_01430"
     CDS             65146..65508
                     /locus_tag="VGA92_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845107.1"
                     /translation="MVEFEKSNFNNIISQIIKKSLFTERQLQIILNKKNLSEFNFNIS
                     KGAYFRQVSQSREKLMGLFYSIILLRGLGVLLPDDIDVISRLAEQVSVIKDSDVFPER
                     EEQVIGVIDKLVRQACGM"
     gene            65633..66199
                     /locus_tag="VGA92_01435"
     CDS             65633..66199
                     /locus_tag="VGA92_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9845108.1"
                     /translation="MLVEIPDPQIIAGVIIAFLVGVFGIIIYNKIKVFAGQKPTDPSY
                     FSRLEYYERQLIDMKIRLDALDLEEKIPLESVEERFEPVLEKKEERVVKKEEKQVEKP
                     AQRPRMPNMGFDGIAEHVLGLITTKEMTSRDIQITIGRSREHTARLMKRLFEEGLVER
                     NTKNKPFTYKITGQGKSKLGNLEHPITA"
     gene            66332..66490
                     /locus_tag="VGA92_01440"
     CDS             66332..66490
                     /locus_tag="VGA92_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9845109.1"
                     /translation="MLSENEVLVKITAVGTISIPKQFRKYLGIQKGDYVKVSLQDDFL
                     VLKRVMIS"
     gene            complement(66487..66873)
                     /locus_tag="VGA92_01445"
     CDS             complement(66487..66873)
                     /locus_tag="VGA92_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845110.1"
                     /translation="MARKRIRIDLEDTDGAKYNFNIEGNITRDKVLKIFEMMDLMNIE
                     EQEAAPQLDSVGAKIWHIVDKYFPMGGFTSNEILEKYEDEYNEPIKLSIISTYLARFN
                     TKGRINRLRNGREWVYQIAKIVQRTD"
     gene            complement(67164..67469)
                     /locus_tag="VGA92_01450"
     CDS             complement(67164..67469)
                     /locus_tag="VGA92_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845111.1"
                     /translation="MGKIGGSDHEKTLDEMVKTLEFDGWRVIKLHGKSPDAIAVKENK
                     IVAVEILKQWRKERTDPIEIKKYGKNQLRYYGGFTMASKRSNYDMFGDVFFKTFQSN"
     gene            67723..68934
                     /locus_tag="VGA92_01455"
     CDS             67723..68934
                     /locus_tag="VGA92_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845112.1"
                     /translation="MSDPIDRLLDAAESGKSLIKNRDVLHFTYIPDVIYHRDEEQEKV
                     TQSLLPILKQSRPSNLLVYGKPGTGKTLVIRKVLSKIQTRVEKSSFPIKMAYVNSKNE
                     TTLYGLLVSFGRQLTQNQSEKDRFPTTGLAISEIFKRILKTINDNHFNTIFVIDEIDF
                     LAHLVSKTGNDILYQLTRANERLKQGSLTLVGISNDLTFKERLDPRVISSLGEEETIF
                     TNYSVDQIKKILEERIKEAFAENTIENSALNLCAAMAGREHGDARRAIDLLRVAGEIA
                     EREQSDKVKEEHIRIASQKMEEDKEVTALRSYPLHEKLLILAVMKASGLSTGEIYAAY
                     KTLCKTTRQKELTQRRITQMLSEIEMSGIISGKIVHQGIHGRTKKYSLTIPTETVKDT
                     FRNEATLEDVI"
     gene            complement(68931..70355)
                     /locus_tag="VGA92_01460"
     CDS             complement(68931..70355)
                     /locus_tag="VGA92_01460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HEX9845113.1"
                     /translation="MIKDLNAALDFALNRGFQVHPDALKILEQVDEKELEGIIRQVVR
                     EKEKQRAYLISQKDLESFLGIKEDEELDDSHEILFDATTKITSAEGVEGFNALFSSRY
                     AKLKKIMSNRPEAKMLKPISSAISIKSKDEIYVCGLVSDRRTDRNVTKITLDDPSGSI
                     ETIVIDEDLQKIASSLLMDQFVMEKIVASKNGGFIVKDIILPDIPDHITNRSKTETYA
                     LFVSDLHIGSKFFMEKEFLDLVSWLSSSDPVARKIRFMVIGGDVVDGIGIYPNQDKEL
                     VALETSEQLRLVFEVLDKIPKHIKVFISPGNHDPGRRALPQPAIPAKYNPNLWGRKNF
                     FMVGNPAMISLNGVKVLIFHGQSVDDIVKTTPGLSYDKPSKVMQCLLKARHLSPIYGA
                     QTPLAPELEDFMVIDEVPDIFHAGHVHVVGFDLYKGVLIVNSGTWQSQTPFQVGIGIM
                     PTSGLAILVNLKTFKVYTKDFASN"
     gene            70466..70987
                     /locus_tag="VGA92_01465"
     CDS             70466..70987
                     /locus_tag="VGA92_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX9845114.1"
                     /translation="MFLAIFSAAFSAGAEMTVPEEEAKIFLDEFNKLLDALQGENFGI
                     EIFLHNIQIALPMFIPGFGVGWGIFSAFSTGFAFAALATTTPLLNEIPPLAMIFATPF
                     GLMELAAYSIGMSRSSLLIISLVKKTPISQQWKQTTIEIGIVIGLLLAGGIIEAYMIE
                     MFSVPENPFSEVK"
     gene            71062..71943
                     /locus_tag="VGA92_01470"
     CDS             71062..71943
                     /locus_tag="VGA92_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845115.1"
                     /translation="MRYVAFAALALVGITLIISQDASAQLAGNKAFSLVGNGFAVSEN
                     DISDTSVDLLFTTNQMNNTIDFSLQTGVIVVGDNDLNISDLSGLLLHKGKIFRIISNA
                     ADSEGNQFTVRILGRIIDTAQTDSIYSLTGTLTDSSKKVTKLVFTTKVSEFTIKTTEK
                     TSKSAITVKILKGSSNPGERTYIDQTAGYTFKFFSEDRITIPVGGTITFVNEDTVSHI
                     LKSGTANYGSRHKTFDPDGKISSGDIQPGQSWSVTFDKPGFYRLFNENYRWMDTTIFV
                     TSETGSQSLGSNIKPHN"
     gene            complement(71940..72644)
                     /gene="cbiE"
                     /locus_tag="VGA92_01475"
     CDS             complement(71940..72644)
                     /gene="cbiE"
                     /locus_tag="VGA92_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX9845116.1"
                     /translation="MITVYAVGVGPGSSAYVTDIVKETIRNSDIIIGYKYTLRTIESL
                     IKDKDVYEITMKDQEETYQKVAKTIGNKKLVVPFTGDVNFSESEVVDRLIEIFGDVQL
                     IPGISSIQVAASKAKVPLDKSKIITMHITTPIEEKKRELQKALIDGFSVILVPRPWPK
                     NPQKHFMQSEIAYYLEKNGFDTSHLRVHVFEFLTTDKETEFVGTVKDLKEKEFSDLSV
                     MVINQAKLESYINFKD"
     gene            complement(72646..73128)
                     /locus_tag="VGA92_01480"
     CDS             complement(72646..73128)
                     /locus_tag="VGA92_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845117.1"
                     /translation="MEPNEKIKAHLLSVWKEEKKLFSKGRECMFFLTNKHVIFITKTE
                     AKTQWWKPAVQRQIITLMKSNDIMLTHDGYGEENLKLDLENKKNEEYPLEDIIDAEAE
                     EKVWGGVLKLKIRHGDKEKKIHLSIAKDWVAYPIRDPVKFLKVDWIPVVEYIKNNQGR
                     "
     gene            73222..>73792
                     /locus_tag="VGA92_01485"
     CDS             73222..>73792
                     /locus_tag="VGA92_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9845118.1"
                     /translation="MSYLQNTLSRQEWLDRTYRKSKSQRALELAQAALKSFDAFCSEK
                     YGKDPDTIISELRGNPGDRTYIFLNNFVAFMDNKSPKTVQTYFGLVKSFLRTQGVKTD
                     RDDVREFVKLPTLLKIRRKPLTKEIIRKLLDNSKERRKALYLTLLSSGMRLGEALSLK
                     KENFDFTKDPVMVFVPAACTKTRESRETFI"
BASE COUNT        21554 a        14445 c        15091 g        22702 t
ORIGIN      
        1 caaaggtgcc taaatcttca acataatcat ttgccttcac agtactattt gatgtggtga
       61 caaacgttgt gccatcccca atatcattcc agattgttcc aggtgaagtt ccaattacag
      121 ggctgctttc catagaatta atatcaacaa ttcttccgcc tccactaatt gcacttatgt
      181 caaatcgcat tcttgtgtcc tgaattatcg catcgtctgg gatagtagag atgttccatt
      241 ctgtggtaag acgtgtacac gcagggctag cggtttggct tgctgctctc attcttagta
      301 ttggcgatcc tacaccagag ctctttgtag taggtgaagg gcagcttgta ccgcttgcac
      361 ctgatgtaga tatgacgatg ttgcttcctg tagaaaaacc aaacactggg tcaagaaata
      421 ctgtctcacc tggttctgct gtttgtgccg aatcaaggaa ttctaaagta gtatccagtt
      481 tgccatttgc attttctaca ttgatttgct tgaattcttt gtgtgccttt gaaaagtcaa
      541 agagtaacga ctttttgcct tctttgttta atgcaaattc cttgaataac tgtgatttag
      601 atttgattag tttttgagaa gatttgagtt ccttgagtgt atctaggatc tcttctttag
      661 catctttatt gaagagtttt tttagttggt tcttgatctt cttttcttgt actttggtgt
      721 ctgttgctac ctgttcgtcg ccaaagtcga tttcatcagc atctaatcct tccatatgag
      781 agacaaatcc aattttggtg ctgactgcgt ttgcatcttt gttggtatag tacaaaaata
      841 cctctaatgg ttgatcaatt tttttgccat ataccgtgag gaagattcct ttttcatttt
      901 ctcttagcgc attaatgaaa gtacctgttg agttttgtgt tttggttaca tgacaaatag
      961 ctgagtttgc agcattttct ttccagtttt ggccgttgtt agattccatc actttccaat
     1021 aatatttatc cacaagtttt gcaagattct tgtttagtct accttgctca aatattgaaa
     1081 cagaacaatc agttttgtca aattcaaatg gaatctgcat cgagtctact gtaatctcag
     1141 tatcagtttc tgctacgatg tgatctacgt atttgtttgt tgcattatca tatatccact
     1201 ctgggagacc aagctcaaca aaccctgttc cgttagtaaa ttcaactttg tagtagtctt
     1261 tgcctctttc ttcaaaggca atatcactat cttgtagtgc ttccaatttt tgcaaggata
     1321 tgctatctac aagcccaaga gcatcaatgt ttgatgtgcc gttagcagac aagccagtat
     1381 ttgtgattaa ttcgacatag tctgcaaacc ctaatgtgtc aacttttgta atatctgtag
     1441 catttgtgag tacatttgtt acgtttaagg tgagtgtgac actgtcagta aatccaagtg
     1501 tttcatctat tatgtgtgat ttgacaaatg ttgcattttg tggaattaat gtgagtgcaa
     1561 tagtatctgc aaatcctatt gtctcttcat ttagaatact agagtttgtg aatgttgtta
     1621 cctctgctgg aatgtatatt agtgtaacaa tgtctgtaaa ggaaagaata ttttctgaaa
     1681 atagtggaaa tgtttgtcgc ggttcaccaa taacttgtga gaatatttgc ttgatttcat
     1741 actctgttaa tgcacggttg taaattattg catcatcaat tgttccagag aatctgtttg
     1801 agagttgtgg ttcttctctt aagatagaga tgtatgcacc aattacaaca tcagtatcag
     1861 attttaccac ctctgcagaa gacgttacaa gtttaccttc tgatacctca attgagtttt
     1921 gcagtgtctt tttttcttgt agtgtgccgt ttacgtataa tgagatctct gttccgtcta
     1981 ttacggctgc aagatgtgag aggctttcaa ttttactttt gccttgtaat tctgtccact
     2041 ttattccatc aaatactgag aatgtggttg tgtgttgtgg tggtaagatg ttgtttagag
     2101 acaatgcaaa tgagccggct tttgatacta ctgtaagttc atctgacaga ttgttgtagt
     2161 ttggatcaac ccatgcagat atggtaagct tgtttgtctg gttaacggta ggtactataa
     2221 catgttctgt tgcgttggta gccagcgtgc cgtttatgtt tgtctggtta agtaggttga
     2281 ttggtggtag ttcgatttga attggaggta aaggtactgg ttttgccgag attaatggta
     2341 gtgttctctg gtataacgcc aagatttgtt gtgcggtaag atatttgtca tagatttcca
     2401 cttcatcaag gcttccagta aatgcttttt gtgccgtatc aactgttctt gtatccaagg
     2461 ttgcaccaac cacaatttca gagtcttttg ccgccatctc aggcgtggta ttctgcctgt
     2521 tacctgaaga atcaagtgtt atggttggaa ttgtttttac cgtttttgag atagtgccgt
     2581 ttacgtaaat tgagatctct gttccgttaa atgtggcagc tatatgagac caactctggc
     2641 ctatttctgt gctggtttgt acgctatgcc actttattcc atcaaataca gaaaaggttg
     2701 cgattcgttg tggatgtacg atgttattta gggctagttc aaatgatttg tccttggata
     2761 gtatggtaaa tacggatgcg ccagaggtat actctggctt tatccaagct gcaacggcta
     2821 gattcgaggt ctgattggtg taattgccgg ctgatttgat aaatccgcca tcaagtatga
     2881 ttcctgtttg gtttacatgt acatcgccta cgaatttaga gtcagttgta gcattcacat
     2941 tccaagattt agtggcattt ggaatgacta tgtggggcag gattgttggg gttgtggcgt
     3001 tagttggagt agcagaattg cctatgatgt ttaggttctc tatgagcgat aggactgttt
     3061 ggaggggggt agccggtgtt gttggggttg tggcgttagt tggagttggt atgaatgtag
     3121 cgctggttgt ttgatttgta gaattagtag ttgttattga agtggtgttt gtagaaatta
     3181 tagtattttc gtaactttct aattccaaag tagaattgga ttcgctcata gtctccccta
     3241 atggatattc tgttatgtta gccaatgcag atgagggtat gatggtttca atatcagatt
     3301 ccaagatatt ttcctgctca aaaatttggt cagagagtga ttcaggaggc atatcatcgg
     3361 taaaattgac atttttttcg gtttcggcat gagcataata tgattcagag attgataatg
     3421 gagtaacaat cagtgatgaa gctaaaagtg aaataaaaac aagcgataat attcgattat
     3481 aagtttgaat ttttaatttt gggttttcag atcttacgat aatgtaaaag gccgcaacgg
     3541 taaaaatgaa aagcgttgta gatgattgtt gctcaaacga atcagtgatg aatctgattt
     3601 gttttccaaa gtcgtcataa gttgattgga ctgcaagtgc ggcaatgatt ggctgtagct
     3661 ttagtaagtc ggtatctaga tcagtagtaa agatgttgtt gatgtcaagt aaaatttgat
     3721 caaagtcatc attttgtatg tcattattat tagtcggtga tcccactata tcatggtata
     3781 atgatacaat tgatccagtg gatatcggta atccaactat gttgtcaaat ttgaattttc
     3841 tatagttgaa tattctttcc agtattgctt ggtagcgatt gtgtttgaga agataaattt
     3901 ggttttccga accatcatca ttagaaattg taatgtcttc tgatatgtga atattagtga
     3961 tgtattcaac tattggttgt cttaatagag ttatagatat ctcctgattt gtcaattgta
     4021 gttttttctc atcaagtgct aatgtgaaat ctcttgttaa ttctgtaaca cttggctttt
     4081 caataaattt tggcggagtt atttcttgtt tataattttg tgatattgta tcaccaaatg
     4141 atgagctaaa tgatgaaagt agaatgaaaa ttaatgatat tgaaatgatt tttgcattat
     4201 tcaaaattat ttacctcatt aaaatttggc agtcatgatt tgtgcaaaac gagaatggaa
     4261 ctgaatttac tgcgtgatat ccagatatag tatccagctg aatatcgtca agattgttac
     4321 tttttgcgtt gttcactgta tgaatgatca gctgaaaaaa tcagataagc ggtgaggaat
     4381 ttttggggaa aaagcaatac cactatggca aattactgta ttgatgatac cagtttggtt
     4441 tacaaaattt caatcaaaat ttttaaaaaa tattttaaga tttagatcac aaataactag
     4501 aaaacaaaaa tttagaattt atgatcaaat aaaatgatca atattaaaaa ttaatttgtt
     4561 ggactggttt tcttattcct actttgtgca aatttgtttg gaatttgttg atatctccag
     4621 agctaaaaat ttttaaagta ttttttttag ctggtgaaaa taatttatga gttacaagtt
     4681 gatttgcaac gttaattgct gggtctaaaa attcaatatg cgggaataac ttttgtaata
     4741 agggaagtaa gaatggtagg tgtgtactcg ataatgttac cacatcgata ttatttgtaa
     4801 tgaatttttt ttgcaagata gaaattattt ttttagcgca aatatttttc ttatgaatga
     4861 atttaccaga ttcaacgagt tcaacaagat cacttgaatc aatttttata atttttattt
     4921 tctttattag attttttttt ataaaattat ttaatgcgga gctttttgca accgaacttg
     4981 taacaagcat agcaatagaa tttgtttttg taagctgttg tgcttccaat agaggtggta
     5041 acactcccat tactctagaa ttattagaaa ttattttatc aagtaataat gatggcgtgt
     5101 tagaacccac aactataagg tctgggttaa attttttttg cagactattg attgtgtttt
     5161 ttattatttt ttcaagttct cttttagatt tttttccata tggaaagttt ttttgatctg
     5221 caaaatagat aatttccgcc tttgttcgtt tttgaattgt tttaattatt gacagagagc
     5281 caaaaccaga atcaaaaaca actattttac ccacatacca cacctccaac tatgcaattt
     5341 agatcttgtc agctaaattt acacatcagt tgattataat gcactatatc aattaagaat
     5401 taaattcaaa acagttgcgc atgaccggct tcgagaagaa ttgggcacgt caagacactg
     5461 caagtttttc agacaaaatc aaagactctg ttaaaccgca aggagcattg aaaccaagaa
     5521 tccaaaccgc agtaaacaaa ctgcaattac aaatttcaaa aatggattca atgctaaaca
     5581 aactgcacga acgtgacgct caacttttta aacgaatcgt agctgcaatg caacaacatg
     5641 acacaagcgc aagtcgcgtg ttatcaaacg aactagctga aatacgaaaa gtaacaaaga
     5701 tgctaagcaa tgcaagaata gcattagaac aagtacagct ccgtttgact actatccacg
     5761 acctgggcga tgctatggta gcaattggtc cagcaatgtc aaccatgaaa ggattgaaat
     5821 catcgcttgg acgattcatg ccagaagctg atgcagagct taatgcaatg acacagacac
     5881 taggtggcct catgatggat tcacttgctg gagactcgtt cagcgttgaa tctgacgtat
     5941 caagtgagga gacagaaaga atattgcaag aagcatcggc agttgcagaa cagcaaatcg
     6001 gggaacgatt cccatcagtc ccctctccct caggactttc gtcccaatca agctcttcta
     6061 cgtacgagta gagtttgaag gcgaaaaatt cctttttgtt ttcttaaatt tttgttaatg
     6121 tattaaaaat attatttgtt atgtatctgt tctcttacta ctttgccgtt tggtttttct
     6181 tctttgaata ttattccttc aaaagaaaag atctttgtct gcttgtcctt ccagcagtcg
     6241 cttggaagac ccgccttttg gcaggtaaaa tccaaaaact cttcttggct ccaaccgtat
     6301 tctactggga cctgtggcaa aagaaggcca gagcgatatc cctgcttgac tattaggcca
     6361 tctctaccta ctttgatcct agatggaagc tgtaatggat catttacttt aagctcaact
     6421 ggtggggtaa gtacggttac ctcaaaagtg atcttatcta gctctgccga tttaactgga
     6481 ggaaatcttg gatcctgtgt tgcagcagcg attgctgcct caggtaatgc ttcgtacaat
     6541 ttttttggca ttgggaatcc aacacaccca cgaagctcat cttgcaaatc tagcgtgaca
     6601 aataatccag actccaagga aagatgtgat ctcagatttt catccagttc tagcttgcgg
     6661 ctgtttgtga caaactctgt tactatcttt cttgcagttt gaacaagaat ctctccgtct
     6721 tggtctgata cattatagct ggccatttat ttctgcaatc aatttttcca agtttctaat
     6781 atgagttccc tcccagtaga tgtgcttaca gtcctcacaa atccagaatt cttttacgtt
     6841 ttgagctatt tttggtggta ctttgtcgat aatctggtgt ttttcgactc catgtaagat
     6901 tccattacat atggggcatc ttgcgatgtt tggattgaat tcatatttcg taaagccagt
     6961 cttttttgca atttctatta gctgatcttt ttccgtatgt gtggtgatat caactgtcaa
     7021 aatatcatgc tttttggcat tgtctgcaac ctgatggtcc tttgtgatta tggcgcggtt
     7081 ttcttttttt gcaagcaaaa ttagatcatc gtcgtcaatc gtggcaaaat acttggagtc
     7141 aaagcctaaa attcgcattt tttttgcaat agttccaagc atcgaatcta ttacaaagct
     7201 agggttttgg ctcaagtttt gacaatccga catctactat gcggcttcca cctgcaggca
     7261 aaacccgcac gtaatcatct atggtaaaat gttttgcaac atcttcgttt aatttgataa
     7321 attcaagccg aattcttttt gcaacctcgg gcatctcaga gtctcctggc tcaattattg
     7381 cataacaaag acagctgttc tttattggct ctggtggtcc acacgtaatt aggtggtatc
     7441 catctctctc tagtatccca actgccaatc ttagattcag aactttgacg aaatttttct
     7501 ggccttctag gacaaaggaa ccccttgaaa gaaactgtcc gcttggcgct gcttttttta
     7561 cttgatctgg gtttatccag tatgcactca caccgtacat tgcctctcgc catgccctgc
     7621 taaaacacgc agttgcgtgc gcaacctcat ttagtgaatc aaacggaatg gactctggaa
     7681 cgtcttttag tatgaaaaat ggtgagccaa agatttctgc atgaaaaacc ttgtcgtttt
     7741 tttcaagatg ctttcgaata attgcagaat ttgatgatga atcccttcca cctatggcta
     7801 ggaatccgtc tgaggtgtaa aaccacctgt atcgctcaaa ccagtttttc tttctaacct
     7861 gggtaaatga gacggatcct tttgcatcaa gtgactgtga tttgagcttt acaatctctt
     7921 tttggttttt tgcctttagt gattcaattg aagctattgc agcagcttgg cgctttgatt
     7981 catcaaataa tgtggatgca attgcaggaa ttgaagcatc ggttcggatc tgaatttttt
     8041 cgccatcaat tttgatataa tctagtcctt tttcttttac tatctcggta ttttgtgatt
     8101 tgagtatttc agctgctgcg ggatcaatta tcgtagttat gccgctggag cttagctcaa
     8161 aaagggattt tgcgacactt gaaatttttt cagagagttc ttttacttga gaggttgcct
     8221 tgttctgttc atctattctg ctttgtatgt cgtttatttt tttatcaagc tcgcttgatt
     8281 gtgaaacttt acccctttct agcagaatct ttgagaacac tttgtccaga ccctccatga
     8341 agcttggaat tttttcatat tcagagtctt gtgcattcaa aataattgga tatacatcaa
     8401 ggctgtcttt ggttttgaca attactggtt cgtggttgcc actcacaact ctctgtgtta
     8461 gcgctctagc agctgtgact atctctttgg ccttatcatc tgtgagaccg ctaccgtgtg
     8521 tgtcaaaatc gacttgggcg actcgaagta tttcttctgc atattttgag ggcaggccaa
     8581 aagttcttcc aaaccattta actatgggag ttgagctaga cttgagctca acaaagtctt
     8641 tttcgttgat atcaagtatg ttgaggcctt tttgtggtgg aggggtgtat gccatgccaa
     8701 cctgtagctc tctgtgccgt acttcgattg aatgaagtag ggccaaaatt ttgagatcct
     8761 tattacacag tatgatgtta ccatcaccaa aaaactcgca aattaacacg aactctttat
     8821 caaaaccaga aaatctcaga tacgcgattc tctcaagacc aatttgttct agttttacta
     8881 gctttaatcg gatcagatca ttgcgcaatc gccttaccag cctgttttcc tcgattttat
     8941 caattttaat ggtgctaagc cacattccaa ttgtggatac caccaaaaac aagtctgcat
     9001 tctgcggatg gtgcagcttg aatagtatgc tgtcttggtt tatcgaatag atattgttta
     9061 catagtaacc gcttgttttc tttgatatct cgcctacaag aaaggtcagt tctattccag
     9121 ctagtgccat aatgaaccag taaaaatctg aaatatactc ttactgatgt ggaatatgcg
     9181 accaaacatt ttaaaccgga cactattgag tcatcctaga aatttcattg acaaaacacc
     9241 caaagaagaa atcaccacca caagacgatt ctaaaaaaga cgaggatcag ccaaagactg
     9301 aggctgctga gaaccaagac gaggtaacta ggcgtaaaaa gctacttgat aagctgtttt
     9361 ggattagaat gggtcttgca gcaatggctg gcgttgccgc gacatttctt tttgaatcaa
     9421 ttgatggcga ggaaagaaga tgggcatcaa ttggttttat gatcgtaact ttcatagctt
     9481 ctgtagtgat tggaaaggga atgagaatag ggcttccgtc atccgatagg aaaaagcttg
     9541 tcacaactgg aattggaagc tatgtgttca tctatctgtt catgtggatc ctctcatata
     9601 ctcttacaca tcatccagac acgggcttga ttccactaaa tccgctttag agtcacttat
     9661 gtggaaatac aaaactcccg gcatacctga tgaatatttt gatagaacag aagaggttcc
     9721 aataacaaaa gaagaagtca gggcagtcca aatcagtaaa gcaagactgg catctgggaa
     9781 tttagtttat gacattggtt gtggaagcgg ctccataaca atagaggcgg cactgcaggt
     9841 aggggagcag ggaaaaatct atgcagtcga cattgatcca aaggcagtcg agcttacacg
     9901 taaaaatctt gctaaattcc aaataacaaa cgctgagatc atattaggtg atgcaaaaga
     9961 aaagattgct gcacttccaa tggcagatgc aattttcgtt ggaggaacag gtggcgacac
    10021 aaaagacatc gtcaaattat gtaatcaaaa actaaagtcg ggcgcaagaa tcataattgg
    10081 aataatacta attgagacac tgtatacagt tttggatgta atatacaagt tggactttgg
    10141 tgatattgac attacacaga ttaccgtctc aaagagcaaa aagacctcaa ctggaacgat
    10201 gatgcttgca cgaaaccccg taacaataat ttctgcgaca aaaaactagt ccaattcgct
    10261 tttaactagg aaaattttga ttcaaggcat gcacgagctt gtctgcgttg gatgcggtcc
    10321 tggagatccc gaattactta cagtcaaggc agtaaaggca atacaaagtg cccaagttat
    10381 tgcgtgccct actgcaaagg aaggcaaacc aagcatagcg cttgccgttg tggaatcgat
    10441 aattgacaag tcaaaaccag agatactgaa tctagtcttt ccaatggtaa aggacaagga
    10501 aacactagag tctacatggg aaaaaaatac caaaatttta gctcaaaagg tgcttgaggg
    10561 gaaaaaagta gtctatctta cagttggcga tccatacctg tacagtacgt ggatctatct
    10621 tcacaggaaa atgcaaatca aatatccgga gataaagata gcagtaattc caggaattgt
    10681 ttcaatgttt acgtttgcat caaaggttgg actaagtctt gcagaaggtg ctgagaccat
    10741 ggcagtaatt ccttcatgtt atgatctatc aagagtaaaa gaaactgcaa aaaactgtga
    10801 tacaatgata tttctcaaag atggaagata tttcgatcag gtaatcaagc tattaaagga
    10861 ggcaggattt tcagatgatt ctatttttgc aattggtcag gatcttggta caccaaatga
    10921 aatagtaaga aagatgagat taggcgatgt aaatgaaaac acaatgacta cgaaatattt
    10981 ttcgataatg gtggtaaaac gtgtataaag tttactttgt agggtgtgga cctggcgatc
    11041 cagatctcat cacagtaaag gcaaagaaac tcctctcaaa agcagatgta gttgtgtatt
    11101 ctggttctct gatcccacaa caaatactca aaatctgcaa aaaggcaaaa ctttacgatg
    11161 cttcaggtct tgtacgcgag gagatctttg aaattttgaa gaataatgca cagaaaaaca
    11221 aacttgtgat acgactccac gacggcgatc ctgccatata tggagcaata cgggagcaga
    11281 cagacaacct gaaaaaggaa ggaatagtat gtgaaattgt accaggtgtt acgtcgtttt
    11341 tagcctcatc tgcggcactt ggattgcagc ttaccttgcc aggtgtaact caaaccatca
    11401 taatcacaag ggcagaaaag cgtaccaaag tacctcaaca ggagcgaatt tcagagcttg
    11461 ccaagcacaa atcgactatg atcttctacc tcagcgttca tttgttgtca gatatagtaa
    11521 aggaagcaat caagggaggg tactccaaat caacacctgt agccattgta tacaaggcaa
    11581 gctggcctga tcaaaaaata atcactggaa ccctagaaga cattgtcaaa aaagtctggg
    11641 ccgaaaaaat aacaaaaact gccatagtca tgattggaga tgtaatacaa ccaaagtcgt
    11701 acgagtattc taaactatac gataaaacat ttagtcatgg gtttagaaag gcaaagaaga
    11761 atctacaatg atatttcaca atatctcata aattaaaata aaaaagaaag agtgttttag
    11821 gacttagttg aatgtgcctg ttccgcctac gttgatggtc cactgtacag tcagcgagtc
    11881 gccgttgttc agtgagattg tttggaatac ttgtcttgca aacattgcgt cagtgctttc
    11941 atttccatct gttgcgttaa atagaccaga ttcgcttaca cttcttgcag cgcctgcagt
    12001 gtttgtgaat gtagctgaca ttactacact tgcttctgtg ctacctgtgg cattagtcca
    12061 accagtaaca gaatttacgt tgccaaacat tctggcaagt ccgttctcaa gaccgtatga
    12121 ggttgagttg tgtgggttgt agagtctaat gtctgtatct agtggggctg ctggtgtgcc
    12181 tgcttctgcg ttgctagtgt ttcctattgc aatccatctg aatccagcat cgtttgtacc
    12241 tacacaaact gtatttccag atgcatcacc gccggttgct gcaaacaata gttttaacgc
    12301 acagttttct cctccgttta cgatcaggtt gtcagattgt ctgtatccta tgacgttacc
    12361 tgcggcgtct tcgtgtattg tagttacgtg tccagtaaat aatcctgtgg cagctgtatt
    12421 agtttgagca tcagtctttg tcccaactag accaaaaatg gttgagctga acaagcctgc
    12481 tactactgct aatgcaacac caagaattag tgctgtagta ctcaatttca ttaaggaatg
    12541 gatccttttc cgctatatag tgattgtgga attttattaa ataatatcgt tccaaagtgg
    12601 taatttttag ctatattggc atattgtgta ttgcagccaa aaatatatct tgatttttta
    12661 tctaaaaaca ctagattgaa aacgcgtgta accattttca tgttgttttt tttgggattc
    12721 actaatgttt acgcttttga gcccatacca ataacagttt caggtacaat ggataagata
    12781 gtttttgatg gaaaatggac gcatgaattt gaatggaagg catcaagtct tgacacttat
    12841 cattacgaca ataacacaga agttattcta agaagtgcac accaaggcga ctttgtttac
    12901 atatttttgg atccaataac cgattatcaa ctagatgaag taagtgatta tgcgatcatc
    12961 tgctttgaca caaagaacaa caaatcaaca aaggcagact ttgatgatta ttgttttatg
    13021 acatacctgg atagcaaaaa cagctttgtc taccatggcg gtcatgcttc agagacaact
    13081 agttttttca atgaaatccc caatcctgaa ggatttattg ggataagtac tgtctctgat
    13141 gagaatgata ggtatacatc agtacctcat ccaagctatg agtttaggat tcctacagat
    13201 ttgatcggaa gagaaagcgt ttatgggttt tatttccttg tttatgatgg tcattcaaac
    13261 aaaacttaca cttatccaca aaatttggat cctgaaggct ttgtctcaaa tccaagtcag
    13321 tggggagaga tatactcgcc agataaatca ctgccagaat ttgaattgcc tttgttatcc
    13381 ttggttttgg cgatcttgtc agttgtgttt ttttcaagaa taaaacatag aatcgctagt
    13441 acaaattaat cagatgagga tacgatctat ttccaaatta aacaaacaaa tcattaaatg
    13501 ccaaaagtgc cctcgccttg ctgcatacat cagaaaagtg gccgctgaaa aagtaaaacg
    13561 atacagacat gaaaaatact ggggaaggcc attaggtggt tttggcgatc ccaacgctaa
    13621 attgttagtc ataggactgg ctcctgctgc ccatggtgga aaccggacag gtagaatgtt
    13681 tacaggtgat agttcaggag actgggtagc aagggttctc tttgggaatg gttttgccac
    13741 aaaggcaaca agcacgcata tcgatgatgg atttagcctc attaatgcat acattactgc
    13801 agcagtaaaa tgcgcaccac cagataacaa acctaccaag caggaaatca gtacgtgttc
    13861 tgagtatctt gtacaggaat tacttgtttt ggaaaatgtc aaagtgatag tatgtttagg
    13921 taagatagca tttgatactc taaaacaact tcttggaatt aaatatcaga aattttttca
    13981 tggaaacaag ttttcattta acaacaagat aattttgtgt agctatcatc ctagcagaca
    14041 gaacacgcag acaggcaggt taacatggga tcaatggaac aaaattttct tgcaagctaa
    14101 aaccctgtta aatgattaat gtgaatctat agaattgttc cagattccgc atctagcgtt
    14161 atttgttttc cgtcctcaag tgagtcgtat tcttgttctg ttgcaatgac tagtgggatg
    14221 tttgcaagtg cacatcccga tgcaactgtg agatcgactt ttttgcacac catcccaagt
    14281 ggggcagagt tgtttgactt gagtgagtat atggtatatg caccaacact gcttccaacc
    14341 ccgtggggaa agacaaggat tgtgtctttt attgattttc ctgccaagtc gtgtctgttg
    14401 tcgtttatga ctccggtctt tttgtcaaca gagccaagaa agttaattgg cgtgtttgtt
    14461 ttgagaattt ttccctgtgt ttttcccttc actattacct tgagtgtcat ttcgtctcgt
    14521 ctttgatgat ttgcgatagt ggttttaggt tgacatcaac gccgtttgaa ttcctcagat
    14581 agtacgcgcc tttgatgcta ttggtggtaa cagagtcaac gtcgtctttg tcaattaacg
    14641 gggtaaggca ggtgcaacag tccgataaaa tttcacagcc tgcgcgttcc agctcatttg
    14701 tatatccaag atttcttgct tgttcttgta ctgcccttgg gcaaaaaacc atacagcgtt
    14761 tcctaaatga tttgcctttg agcatcaaag aaaggctaga catctcttct agccctagct
    14821 gcgggctgcc aagtgtgatt aaatccccac ggtctgatgt gtttagctca tcgtaaattt
    14881 tctgcatctc ttttttgtca aaatcaacct tttcagagtc ttgtgtccct tctcctaaga
    14941 caaacttacc gcatctgccc gacgtcccca ttcctccaca caatgacttg cagctacgct
    15001 tgtccatttc tgagacgcct gaaattgcaa cagagttatc agcaattttt cctgcaaaat
    15061 atccaagcat gccaaatgcc atctcgtctg gctgctctac cttcattcga attgtgaggt
    15121 ttggcttatc gttaattcta agatcagagt aggggctttt tccagtcagc gcactagcta
    15181 gtgcgctaaa cgagctttcc ttgtttgttt ttaggttggc aatcgagttt gcatagattg
    15241 cagcgttgct ttctgcaaaa gacacctgcg ttcccttttg cggcagatca aatatctcgt
    15301 acggaatgca tgaaaacgac ggagttgttc ctattttctg ataagactgt acaatgcttt
    15361 tctgtttttt tatgaattcg tctgaaagtt tgtaatctga gaccttttca aagtcatacc
    15421 caattgggtt taccgttgtc atcaccttta cctttgcgct ttgactgagt tttgagagaa
    15481 attcctctcc ggcatcgcca atcgtgttgt agttggcccc agaaagatga gcccacttga
    15541 ttggtactag cttttctgcg tttgttgcct cgcctgttgc gataagaatt ctgtacgcag
    15601 tctccagcgt ctctccatat tctcccttta gtgcaagttc ttcttctttt gttagctcca
    15661 caagtatggt tgcattgagc gatataatac ttctatggaa attttcaagt ttaacttatt
    15721 atggcataat gcatctcaga aaataatgga gaggattctt agcggcatga taaatacaat
    15781 gaattccgtc aagccaccaa gaatgactgc actgcgccag ctccatgacg tagaaaaagg
    15841 agacccattt gcaatcctaa ttggaacaat tctttccgca aggacaaagg acgaaaacac
    15901 aacaaaggtg gtcggccgat tgttcttgca atacaaaaca tcaaaagact tggcaaatgc
    15961 aaaaacaaaa gacgtggaaa aaataatcag gccgattgga ttttaccacg taaaggcaaa
    16021 aagaataatc gatgttgcaa aaataatcga gtcacagtat gatggagttg tgcctgataa
    16081 tttggaccag cttgtgcagc tgccaggagt tgggcgcaaa acagcaaact gcgtactagt
    16141 ttatgcctat gaaaaaccag ccattccagt agacgttcat gtacacagaa tatcaaacag
    16201 attaggtcta gttgagacaa aaactccaga agaaacagag cttgccctga ttaaaaaaat
    16261 tccaaaaaaa tactggctta aaatcaatga tacctttgta atgtacggcc aaaacatctg
    16321 caaacccatc tcaccgatgt gtaatgtctg caagatcaaa aaatactgca aatactacaa
    16381 gactaggaac gcttcttgac tagcaacaac acgccaatta ttgcgccaac tgcaccagtc
    16441 acataatacg gataataatg gattgggttt tgtgatacat cgcccgatat tatatcaatg
    16501 atgaatgtgc cagagtttat tgcaggcggg tcagttggct cattcattcc atacaccgca
    16561 tagctgccaa caacaaagtt atctaagata acgttactta caacgattct ctttccattg
    16621 cctaccgaaa tgccttgaga atcactatat gctgcaataa ttttcgagtc ttcataccat
    16681 ccgtccttat caagtcgtgt ggcaggaatc aagccccgct ccggatcatc aattgtgacc
    16741 cagaatttac gatcgggcgg gctgcccatc cctatctcga tgaacttgtc tgcaatgttt
    16801 gcatcgtaaa ttcgtattat tgcactcccc tttggatttc cgtaaagaag gtcattttct
    16861 atgtttatct gccagtttcc agcatgagcc gtatcaaatg gtataggtgt agaatcgtct
    16921 agaaccctgt tgaactcgtt tgccggtatg tctatttttt gtaataccag tgaggtgttt
    16981 gaaaactctt ggccgtatgc tggcacaaat gccgcaatta tggatatcag taaaaccagt
    17041 tttgatagtt tcatgcttat tccacaaatg gttttttgtg tgagaggatt actttgatga
    17101 cttcgggcct cattatgaac tccgaaggcg attggccctc atcaattagt gcacggagtt
    17161 ttgttccgct tatctgctca tggaattcag gcgagtgtgg acatgcacgc tcgtttgtaa
    17221 acgcaaggca ttttttgcaa taatagaatg ccggaaaaaa gataggcctt attgccatgt
    17281 ccgtatagtc agaaaagatc tcctgagctg caaacggcga gtagtatttt ccgacacctg
    17341 cgtgatctcg tccaattatg atgtgtgtac agccatagtt ttgtctcatt attgcgtggt
    17401 gaattgcctc ttttggtcca gcatatctca tctcggtgtg tagcgttgca agaatgcatc
    17461 tgttttttgg gtaataatta tcaatcatgg ccaaataaga ctccaagatc acctcatcaa
    17521 caaagtcgcc tgactttttc ttcccaatga gtggattgac aaaaacacca tcgtgtgtag
    17581 taagcgaggt tttttgcagc atctcgtgcg ctacgtgtgg cggatttctt gtctgaaacg
    17641 caacaattgt tttccatccc gcatctgaga acgcttttct tgtttgctgt ggcgtcattc
    17701 tatattttcg aatcgagtct tcccctggtt ttctgatgta gtcgattttc ccaccaacca
    17761 gatactcgtt catcgagttt gtttttgcta cgccgggatg gtttgcatca gttgttccat
    17821 aaatggcctg agcagccttt tgcttgtcaa atgaatacac atcttcaatg tgcaatatag
    17881 caaattcgcc atttgagttc tttagtgcca cgtcacctgc tcgcttgatc ttatttgcag
    17941 tgtctttgtc gacatccaaa acaattggca ctgtccacgc aaggtcgttt gcaagccttc
    18001 ctttttttat aacactttca aagtcggcct gaagcaaaaa tccttcaagg gggctgaaaa
    18061 tcccgtctgc gatgttttct acgtcatttg ccaagtcttc acttattgaa atagaataca
    18121 gtccgtctgg atttttttgt gcaattctgt ttatcagcct cccaccgtga ggcttgataa
    18181 cgccttcatg ttcagtcatt gctcagagtg atccatgtgg aggccacatt ccttgtgtgt
    18241 atcagactcc caccaccaac gtcctgctcg caggtcctct cctggtttta tcgctctagt
    18301 acatggctca cacccgatgc ttgtgtagcc tttgtcaagt agcttgttgt atggtatttt
    18361 gcgttctttg atgtagttta gtgtctgatc ccatgtccaa tcaattaatg ggttaatttt
    18421 gattattcct ccatgaaggc tgtctacttc aaccatttct atcattgatc tgttttcgtt
    18481 ttggtctcgt cgcagtcctg taatccacgc atcaagcgta cttagaattt tgtttagtgg
    18541 atggaccttt cttatctcac aacagagctt tctgttttct acgctttcat agaaaagatt
    18601 tattcccttt attgtcacca tctcctgaac ctcgtttgcg tcaggaaaca tcacctcgaa
    18661 tttgatgttg tatttttttg caagctcgtc cattatgtcg tacgtttctt ggtttagtcg
    18721 tcctgtgtca agtgtgaaaa atctggcctt tggattaatc ttggttatca tgtcagtaac
    18781 tacggaatct tctgccccaa agcttgacgc ttttgcgatt ttgtcgccaa aagtatcata
    18841 tgcccacctc agagcatcct caggcgtttt taacttggag ttaattttgt caatttcttc
    18901 ttgcgagaat cttgccacat tgattttggt cgtaagttgt tttataatta ttactggata
    18961 gtagacaagt aaatcaaaat tataaacagg caaagtccat gaaagaaaat gaacgaggct
    19021 gcctttgtag ttgttttccc ttcgattttt gccaaaaaca agcaaaacca actaatcaca
    19081 aatatcaaga aaattctcaa aattcaaaac cagcactttg gccaacttcg aaaagatgac
    19141 ggcctgatag tgattgacgc aaatgacccc gtttttgcct cctccgcaat taacctgctt
    19201 tttggcatcg acaaaataac aatatcaaga cgtactgaaa acaggtttga tcttctagtc
    19261 tcctcaatag caaagattgg cgcaaacctt ttgctaaagg gggaccaatt ccacatcaaa
    19321 gtagagggcc actcctctgg ctatttgcca aaggacgtag aggttgccgc aacatctgca
    19381 ctaattgaga aaactcacca tatggactgc aggcctggaa cagaggaaaa acatgacaag
    19441 cagatctact gctttcttac aaaaaagaac gcctacattt cgatcttttt agacagcggc
    19501 catggtggaa taccatacaa ctcccaaggc gaaaaaattg tctgctgcat ctttgatgag
    19561 ctatcagcag taggctgcct tgagtcaatc aagcagggat ttgatgttag gattctagtc
    19621 tgctacaacg attctaattt gcttgagcta gtcaaaatga taaatagaat tctgccaaga
    19681 actgtcagca ctcaggttac gctagacttt ttcaggctcc cattaaagca ggaaaatgca
    19741 aagtcgctcc agctcaaagt cagggcagta actgagattt tgtgcgctgc tgcaaaaaaa
    19801 gaaaaaatca aaagaatatc tctaccgctt tctccgcttg tgtttcccat ttggttcatt
    19861 gatcaaaatg tcagcaccat actccaaaac aggcttgtac catggattgc gcttgcagga
    19921 gttgacaatg ctataatcaa tacggcaaaa gagatcggcc ttggcaaata tctgcacaaa
    19981 atcgaaaggc tcggcacact cagatttgca aaaaacgatt cggatgtttc aggcattgtt
    20041 cagaataccc taaagacaag gcagtcaata actgtggatg tcgggccgaa taacactcac
    20101 gacattttgg actcgttaaa gcattgaaaa tttatgcccg ctctttttga gtgaggctgt
    20161 gaagagaata ggccagttca tctattcgtg gggcaacgga cactactccc gaatgatgtc
    20221 gcttaatgag gagctgccaa actttgtcaa agacgagtac gagattcatt actccagcaa
    20281 ggacgagatc taccaaaagc tgctaaagca gtttccagac aaaaagcaaa acatccatga
    20341 gattctgatg ccaacgccaa ttgacggaaa atacggccca agtatttttt tatcaatgct
    20401 aaacgttttg ttgcccatat ctggcaaccc gccagtagta aggcagatca gcagttattt
    20461 gagaaaagag gccaagttgt ttgattccat tggatttgat ctagttatca acgacggcga
    20521 tatgggccca aacatcttag catacaaccg aggagtaaac tgcatttttg tgacaaacca
    20581 gtttatgcca aaattgtgga aatcccgttt ctatttttat ccaggtgtcg tatttgtcgc
    20641 aaagcaaatt gccaaggcaa caaagattgt tgttgcagac tcgcctccgc catatactat
    20701 gtgtgaatac aacctgaact tcccagaaaa actcaaagaa aagatagttt acacaggcca
    20761 ttttgcagct gataaaaaac tcagatcagc tcaaaagaca gatcttgaga ggctaattga
    20821 gggcaatgaa tttggctact ggatgaggac tggcaacaag tccaccaacc agataactgg
    20881 caaaaaatat gaagaggttt ttacatcgcc agagatgaaa aacgaaaaaa gaataatttc
    20941 gcacgctaca agtgatcctc caatagacag agttcttgga atggatggca ggacctattc
    21001 tgtttgcgag gcgctagaaa agaaaatcga ctgggttcaa atcgacattg ggtttttgtc
    21061 agaacagcaa aaggatacag ctcttgagct ctgcaagtac gcagtcataa acggctcaca
    21121 tacagtaatg ggcgagataa ttggggtaaa aggcaagcca ataattggca ttcccgtata
    21181 tgacgagcag tcaaaccaga taagatgggc gcaagagcac agactcggaa ttggcgcaaa
    21241 taacaaaaaa caagtcataa cagcaattct ggagctgaaa aaaaactatg ccaagttcga
    21301 agaatcaata caggaattcc gcaaaaactt tgtagcaaat ggcgccaaga ccgccgcaag
    21361 gcttgcagca cagatgcttg atgataagag ataatagctt ttttgaagac tttaagattg
    21421 tctggtacgc ggtatttcga tgggcgaact gaccgaaagg gatgacgata taatccgcgt
    21481 accagaactt tgcgatcacg attgggaacg cttttatgga aaaatttttt gacaaaggca
    21541 ttggtaaatt gcccagaatg tgttgcaaaa gaacgcaagg cagcatttga gcgccaggct
    21601 gcaagtggaa gccaagaagg actccaaaag gtcatagaag aaattgagat tcctatgaaa
    21661 ttcgatcctg taacaaaaca cttcatctgc aaaagatgtg ggctttatgc caccagagaa
    21721 caggtccacg atatcaaaga ccgactaaat caaagagagc gtacacgaga agacaagcat
    21781 tatgactatc ttgagtggtg gcaaaaaagt aaaaaagaca aaaaatagag agggtgcctt
    21841 atggtaaaaa ccaaggatga gctgccagac tgggtattag atgagataag aaacgccaag
    21901 tttgccaagt cagaacccat tacgaggact ggatacattc tcgaagtata cgatgatgat
    21961 aaaaaaatcg acgcccagat ctatgaggca gtcgaagatg gtcgtcacat agtcaccttg
    22021 gatctaccaa aaaacatcaa accagatgac tgccaaaaag gcgtagtgta cgagtttaat
    22081 ttcgatttgc tcaaagtatc acttggcaaa aaagtagttg actttctaaa gaatgaaaaa
    22141 gaaatcgaaa tgcctgcaat ataccagttt gcgttacaaa aaatggagtt aatcgatgtt
    22201 ggctcagacg aagctccgtc tgagagcgaa gaatagaatt atttttttgc catctgtttt
    22261 ctaaatgcgc ttccaagaat tgggtttatg atgtagggaa aggtgtgctt tgcccacact
    22321 gctcctcgga ctatcgacgg tacgatgatt tcaagccttg aagaggttgc tgcggagact
    22381 actgcctttg ccacagtttg tgcactcagt gcagtaggcg agtattttgg cattttttca
    22441 aatgactgat gatcaaaaaa gtttgtttta accataattg ggctgacaac ggtcacgccg
    22501 attttggttc cgtgcagctc atgtcgcaat ccctctgaga atccgagcat tgcaaatttt
    22561 gatgcgcaat acgacgcaat tcctggaagg ccaaatgacg ctgcaacaga tgccacatta
    22621 actatgtgac cagaattctg ctctagcatt tttggcaaaa aatacttgat gcagtaaacc
    22681 attccaaaat agtttgttgc catctgggat tcaatgtcct ctattgtcag ctcagaaact
    22741 gttccgtata tggcaaagcc cgcgttgttt actagaacat caactctgcc aaagacatcc
    22801 aagacttctt ttgacatttt ttgggcttgg gatttttgtg agacgtcgca ttcacagaca
    22861 agtgttttga cgccaaattt gccaagattt ttttgcatct cctcaagctt gttttttctt
    22921 cgcgcaacta aaacaaggct tgcgcctttt tttgcaaact cgatgcaaga tgcagcccca
    22981 attccgcttg aggcaccagt taccacaaca actttctgct taaatgacat tgctttgcaa
    23041 attaatcctg gaagataagt tggtttctat tgcagtttct cgtattttct gagtgccatc
    23101 ttcattagta aaaaccatgt aattggaatt ggaagctgat atgttgctat tctccatgct
    23161 atgatagaat tccactctac actgtttttt ggcatctcca gcttgaactc acttagatgg
    23221 ttaagatacg cccacacgcc aagttccgtt agacccgacc cgcctaccgt tattggcagg
    23281 tttcctattg cgtttcctgc catgacggca aagatcgatt caatggcgct gattgcattt
    23341 tcgcttccag ctaaaatcag cataaatgag aggccaaaaa tggcccagga tgcaagtgaa
    23401 attgcaaatg ttacaacaaa ggcctttcgt gtttgctttg agctaatgtt ttctcggctc
    23461 atcgtgcaga tctcattcat ccattggttt gtcttttcga ggtaatgagt gccccgttct
    23521 tttccaaact ttattatgat tttagaaact gctgaaggaa ctctaaatgt aatctttgat
    23581 gaaaagaaaa agagccccgc ccacagactt acaattggaa tggaggttgc caaaacgacg
    23641 accccaatta tggtatagcc gcttgctatt gcgacaatac ccgcactcac agcaagtatt
    23701 ccggctgcaa gaacctcggt tactatctca aatattccaa gccaggaggc cttggcagcc
    23761 gagaggcctc gcttgttcat ccagtagatt ctgactacct cccctccaac aaacatgggc
    23821 gtagtcattg tcacaaactc gcttccgatt ctgacaaaaa tcgttttcca gaatgtctcc
    23881 actgaaccat ggaacttgcg tatgatatag tgaaatttta taccctgcaa gaaaagcttg
    23941 cacatcattg cagcaaaggc cgccaagaaa ttgatcagac ctatcgcaaa aatatcctct
    24001 ggctttacct tgaatgtgac tgcaataatt atgaatggca ccagactagc tagaaaagca
    24061 acaattctcc aattcattta aaaaaaattc tagttgattc aaatgtaaac ttactgttag
    24121 acaatatgcc taaagatatt caatagtgat taattcgaag aaaggatagt attctaagat
    24181 tttataggga aaaagattac tgaattgcca cagcggtgat cgtattagtt aatgatatag
    24241 acaatacagc ttgatctttt ttcgatcata actgttttaa acggattaga tgtctaaatc
    24301 tcaataaaaa atgtcaataa taagaaatta caaatttcga ttgtatccta ctatagaaca
    24361 agcaaaggaa ctacagaata atcttgatgc ctgccgctgg gtatacaata aatttgttga
    24421 atcaattcag agtgaattcg tatcaaggaa tgatcttaac tattttctga ccgaattaaa
    24481 acagtcagaa ccttggcttt acaaatacca tgccaaaatg ctacaaatgg tctcaacgca
    24541 gctggctgga gctcagaagg ctctcattaa gatgcatgaa atagggcgca aaccgggtaa
    24601 cctggaattt accacatata ataaacaccg aactttcacc tacaatcaat tcggatatga
    24661 aataacaaag catggtaaca ccgaccttct atatctgtca aaaattggat ttgtggaaat
    24721 caggttgcat cggaattttg tcggtgaaat caaacaagtc acagtaacaa aatcaaaatc
    24781 aggaaaatgg catgcatgtc tggtgtgcga tgttgatgcg gaactgccga aaatcagcct
    24841 gacaaaatcg gttggaatcg atgttggaat caaaaacttt gcgtatgact ctgacggatt
    24901 tgtaactccg aatcctctca acttacaaaa aatgctaaag cctcttgcaa gaatacaaag
    24961 aaagatctcc agaagacaaa agggatcaaa taaccgcaaa aaagcagtca aattctacca
    25021 gataatccat gaaagaatca aaaacagacg cagggatttt ctgcacaaac tatcgacaca
    25081 gtatgcaaaa aaatacgatg tggtgttcgt ggaaagattg caaaaactga acatggtaaa
    25141 aaatcacagg ttggcaagaa acattctgga ttctggttgg ggcacatttg caaacatgct
    25201 tgattacaaa acaatgctga ttgatgttcc agcaaaaaat acaaccattg attgctcaag
    25261 gtgcggcaat atggtgccaa aatctttggc cattcggact cacagatgta atatgtgtgg
    25321 tttggtgctt gacagagatc ataacgcggc aatcaatatt ttaaaaaaag gattagatat
    25381 ttttggaatc acatatcaag gatcaaacct accgcaggaa ctgcgggaag taacgcctgt
    25441 agagatcaca gagtggtcaa ggaagcagga atgttctacc gctttaattg gtagtaagtt
    25501 cacaagccaa taacaattat tttgagaaat tgaaagtaat ttttgttgca ggtactgctg
    25561 gagctggcaa atccctgctc agctcaaaaa tttaccaata ctataccaga aatggagcgt
    25621 ttgcagcaat tttaaatcta gatcccggtg tgatttctct gccatatact ccagatgtag
    25681 acatacggga tttagtcgac attgtttctt taatgaagca gtatgatctt ggaccaaatg
    25741 gcgcccttat gatggcaaac gatctgattg cctcaaaact agatgagatt caacaacagg
    25801 cagacgatat taatcccgac tatctaatta ttgatacacc gggacaaatc gagctgtttg
    25861 cgtacagggc aagtggtccg tattttgtta acaattttgt agccgagcaa aagacaagtc
    25921 tttttttgtt tgatggcgtg cttatgacga ctgcaacaaa ctttgtttca atggcgcttt
    25981 tggcaacctc aattaagctc aggcttggcc tcccaacaat taatgttctg acaaaaatag
    26041 atttgattgc agacaagatt tctgagattc ttaggtggac tacaaatatc aagacattag
    26101 aaaatgcaat ttctgcagaa gccgatgggg aggcttacgt tcttttaaca aatctgctgc
    26161 gaagcctaaa cataggtggg tttgcgcaag ggctcattcc aatctcaaac gcaagtgggg
    26221 atggcctgat taatctagag gctgccttga gcagaattct taacttggga gaagaggtag
    26281 aggactgatg gcatcaataa cggtaagagc accgtcatct actgcgaatt tgggcccagg
    26341 ctttgatgtc tttgggttgg cactagatgc cttctacgac gaagtaacac taacaaagaa
    26401 aaaggacgga attacaataa ttacttttga taacatcccc acatctgcac aaaaaaacac
    26461 cgcaggcctt gtggtgcaat acatgaaaaa aagattcaaa gtaaaaagcg gcgttcaaat
    26521 caaaatcgaa aagggcgttc cggcaggatt tggaatggga agcagtgcag cgtctgctgc
    26581 agcaactgca gtagcatttg acagactttt tcagcttgga ttagacgata attcactcgt
    26641 tgaatgtgca ggagttggag aaaaggcaag tgcaggctcc attcattacg acaatgtatc
    26701 agcctctgtt ttgggagggt ttgtaatagt taggacaaat ccgcttgatg tgatcaatat
    26761 tctgcctcca aaagatcttc tgctgtgcgt tgccatacca aaacttgatg tgccgccaaa
    26821 gaagacagca gtatctcgga gtgtggttcc aaaaaatgtc aagctcaaag attctgttac
    26881 aaacctctca aatgctgcgg caattgttgc tggatttgta agaggcgacc cggagctaat
    26941 aggtagatca atcaaagacg tcatagtaga gccggcaagg cagcacctaa ttcccgggtt
    27001 ttccgccgta aagaaaaatg cgctcagagc cggggcactt ggcgtcacaa ttagcggcgc
    27061 aggtccttca gttattgcat ttgcaacaaa aaaatccgac ctaaaaaaaa ttgccaaggc
    27121 aatgaaaaac ggttttgcct ctgcaaaaac tgattgtaat gttgttatct gcaagccaac
    27181 caatggagca aggataaaat aatttacaaa gttggaaaaa tgaagaaaaa agcagtagta
    27241 attcttagtg gcggtctgga ttcgctgtgc cttggggccc accttagatc aaagtacgat
    27301 ctctatggga ttacattttc ttatggacag cgcgcctcaa aggagctaaa tgctgccagg
    27361 catgtcggaa aaatcctaaa gctaaaagag cacaaggttg tccgaattga gtttatgaaa
    27421 agtctctatg gccaaagcaa tgttttgaca agcacaaaaa agaagattcc ctcaaaattt
    27481 gattactcga tagttgttcc aatacgaaat gccatctttt tgagcgttgc aactgcgtgg
    27541 gctttttcgc ttagggcttc cctagttgca tatggagccc acaccgatga taaaaaatat
    27601 cccgactgca ggccagcctt ttccaaaaaa ctcgagtcgg ccctaaatga gggagaaata
    27661 gacggaataa ggctcgggct cagaaacaaa atacaaatct ggacgccgta tttggcaggc
    27721 ctatcaaaga gcacgctaat taagacaggc tacaaaaagt ttggcaacaa tattttcaag
    27781 acttggagtt gttattctaa tacagcgctt cactgcggca ggtgcgaatc atgcaacaac
    27841 agaaagcttg cctttttgca ggcaaaaatc aaggacaaaa caaagtatct ctagtatcgg
    27901 gtttctagga ttttcctttt gaaaactagt ggccttattc caatgtacca agcaaggtaa
    27961 attgccccaa ttattccaaa gaaaacccag ttctgcattg ccggatcaaa ggtctgctca
    28021 aacatcctta caacttcgcg cgaagacaag gctgctactg ccatgacaat aaagaactct
    28081 attgcaaacc aagtccagta cggccacgac tcttttggta tgtgaacatt actgtgaact
    28141 cccatgagaa gctatccctt tgattgatta tttaattttt tcagaaaaca gacggttttg
    28201 agttgcggat catagctaca atgtcttcgc ggttttccct tctttcatag actcccctgt
    28261 atgcagccga agtcatagta gcgttgtttt tgactccgcg catcttcatg cacaagtgct
    28321 cagcatctga tagtacgatt gcccccttta ctccttgtga gtacagctcg tctgcaatgt
    28381 cttttgtcat tcgttcttgg atttggaggc gttttgcgtg tttttcaact aggcgtacga
    28441 gttttgatag tccaaagact cggccatttg gcaaatatgc gatacttact ttgccaaaga
    28501 atggaagcat gtggtgctcg cacattgagt aaaagttaat gtcacgtatc actactgcgt
    28561 ctgagtcttc acaaaactgc acagaaagct ccgagtccga atcatatccc gaaaagattt
    28621 cctcatacat gtctgcaatt ctgtctggtg ttccgcgcaa gccctcgcgg gttggatcct
    28681 cgccaatttc gattattagc tcacgtacta gcttttttac tcgttctttg ttcattatgg
    28741 cgctccaatg aatttgtgaa gttggggtac aacgcgcacc tcattatagt atggatatac
    28801 aatgtcataa aaaccgaaaa gttgttgcaa atttggttct gctattccat aggttggctg
    28861 tattataaaa cccttaagat ccttaggatt agctattttg aagatttttg agactagatc
    28921 ctcaaatggt tttggttctg tttttgcgct aaccactatt ttgatgtagg ttgatttctt
    28981 tgatacaatc gcagatttca gacactctag cgcattttct aagagtgtag aataatgttt
    29041 gttatccaca aattccgagt cttgtgtttt gaattcgatt ttaacatagt caatatgtgg
    29101 tagaacctgc gaaaatttct ttgaatcaaa gcaggaggac tctagatatg tcgggatgtt
    29161 tttagaatgc acatgttttg caagctcagc tactgcctcg tgctgaatca gcgggtcgcc
    29221 tcccgtaaag ttgaccttgt aggtctggct ctgcagatgc ttgtcaatta gcgcgcatgc
    29281 gtctgcaagc tcgtattctt ggccagagtc tagcggcagt gcttcttttg tgtcacagta
    29341 aaaacaagta aagggacatc cagccagtct tacaaatagc gtctttgttc catataggat
    29401 tccttctcct tcaattgagg tgaaaatctc atagagcctt agtttcaagt agatgatctt
    29461 gctctaatcg ttatttaatg atactatgaa acgactggct cttttgcata gagcagtcct
    29521 gcgaaaaaaa agaccacacc taaaattcca attagggccc caacaaattc aattgtttca
    29581 atgaactctg tatttgtagg cgcccacaaa aatgccatca acgctcctac gactatcatt
    29641 ggtattccga ctccaccata aatttgccta gaagccatca attttgctga tttacaaaat
    29701 ctatatgaag tttgttctag ttgtttcgtt ctgtgctgct cttttccttt tcgctggatt
    29761 tattgggttg attcttgtcg gctatgtctg atctgtcatc atcattgttt tgcaaagatt
    29821 ctacgtatcg ttctagactg tcaactagtg aatcaagtat ccttactgtc ctaagataat
    29881 cagcatagcc tgatgttccc tgacttgtaa gcttggtaag aagtctttct gatgtttcaa
    29941 acttgaactg gatgttttca tcaacgtacg gtttgatgtt tgcaagcctt gcctcaagtc
    30001 gcaatacttc tgatgagact ttttggtttt ttgtgtcatc ttgtgttgag cgttcttgtt
    30061 ttggttcagc aggtcgctcc tcggatttgg cttgttctgc ttgagcctgt tgtgttgcca
    30121 tctgatcccg tttggccttg gcatagtctt ctatttcttt gactagagaa tccaaatgcc
    30181 tcagtaattt tgtagcttcg tcagcagatg tctggttttt tagctccgcg atggtttgcc
    30241 ttactgcctc aaattttggc ttgattgttt cgtccatgta tggctcaaag tctgcaagtt
    30301 tcgtttcagt tgttgcaatg taatcgttta caaatgatct tatcctctcg ttttggcgct
    30361 ctctggtttg ttcactgatt gcgttttgga catcatttag tgcgactctg agctgaccta
    30421 cgattctctc aatcgaggga atgtcacttg ccgcaagact tgatcttgcg ttttgtatca
    30481 actcttcaac ctttaagaaa tcgactgtaa agtcgttctt tgttactagg tcttttaaca
    30541 tgtcaacata tttttcagtt ctgtcaatgt cgttttggta tctagttaga atggctgggt
    30601 cgacttggga tgtttttagt gttgtaactg cttgctgcgg ttcagaaagc tcgacgctga
    30661 cctgtttgaa tatcttcatt gcagccaaaa agtgttgttt tgcctgcgcc acatcatttt
    30721 gttttactgc agaaatcaac aactcagttt cctggctgcc tcgctcaaag agagccttta
    30781 gctcatctga tacatttgct gtcctttcca gctggaattg tacttgtgtt cttgcctttg
    30841 ttgcaatgtt gactagtgag tcgagtgtag aattggcaaa tgttgttgga agtccaacta
    30901 tcatgcttgc aactagggca attagtgtaa atgtcagaat ctgtttcatt gttccccctc
    30961 cccttttctt agcttaacta ggttttgaaa gtcttttttc tctatcacaa taacaccctg
    31021 cctctcaagc ctcttgactg ctcgccacat tgtcgtgcgt ggtaaaagga acttttttcg
    31081 tagttctttt tcgaacgcct ggccgccatt ttgctccaaa aaggcgacaa gctgcttgtc
    31141 gtcttctctc atctgctggg tagtttcctc agactctatc tcagattcct ctcgcgccat
    31201 gatcggctct attttctttt tgactgcgct ttttattctc ttgaagacta gtgtgattgt
    31261 ggtgattgca ccaccaatta ctgcaatcga agccgcaatt ccaattatgt tgtcgcttag
    31321 tgttgagatg tcgcctggca ataccgtgtt gtttttagct gtttgttgtt cttgtaatgc
    31381 aatctgttgt gcttcaagtg ctagcttttc ggcatcagca tattgcctgc tctcaaatga
    31441 caagactgcc tcttccagtt ttgctgttgc ttgcggggtt ttgattccaa tgtcatttat
    31501 ctgctcaatt agactttttg atttctcaat attatttgag gcagattgtg ctgggcctga
    31561 gacaccaaag aaatagtcga tttgattttc tccttttgga agtgagaggt gaggcttgtt
    31621 gttaatggtt tcaaccaagt ttggaaagtt cgtcattcct acaattactg tgtcttctgg
    31681 catcgtcatg gtataatcaa caggtgaatc aatttcaaat gtccagatct tgccttgttt
    31741 tgatacaaga tcaaaagtat catagtcgat agaaacgtta gttgccccaa atgtctggat
    31801 gactacttga ttttcgccaa tctcatataa caataacaag ccgttttcgt cttgtgcaac
    31861 aaaattatca attacgctgc caaagagtgg gatcgaaagt tcagggtttt gtggatctgc
    31921 tgatgcctct tgagaaacat gagttgtgcc atctggataa atggtaaatt ccgcggtgcg
    31981 cagcaagcca aaagtgtgtt gagtaggcaa taacactgca aggataatac ctatcgctag
    32041 taatggcgct tttagcattc taatgaggat atctgtaatc cttacaaaaa gcattccctc
    32101 tatgtctgga atgtcatgta tgctacagag cactgcctct tgggttttca tggtcaatac
    32161 cttaaattga gcgaatctag gagcgactgg aactttctga actcttggac aaactcaatg
    32221 cctttttcag ttatcatata aaggcggttt ttgtcggttc gtacggattg taccaagcct
    32281 gcagttgaga gcttttcaca cttttcgatt acagcatagt gtgaaaggtt tgctcgtctg
    32341 gagattgcag aaacaatagt tccttgtctt cctccttcca ttgtgacatt taggatgtca
    32401 cccattatgc ccatttcgga cctgtattgt tgttttgaca tggtttagta atagtgagga
    32461 tgattgatta gggacgtttt gaaaccgcaa agcggaacac aaagcggaac aggtgtttca
    32521 ggtactgaaa caccaaaaaa atggcatatt tttgtgcggg gtagacaaaa acaagagata
    32581 tgccaactag aatttttctc aaaaacagtc ctttttttga ctttatattt attaggctac
    32641 ggtctgtata gtttacaaga attcgtgata caatgtttga aagatttaag cggggcgata
    32701 cctcgcatga agatgctgga gttatggaac aagaaagacc agatactaat tcagaaaacg
    32761 tggcggaaca ggtaagaata cctgaaccac cgtcgagttc tggctccgat attggcatta
    32821 gtgagcttat gagtaaacgc gccaagttgg aagaagcaat tgattatgtc ggccttatga
    32881 tcaaaaacct caaggacaaa cgaaccaagc ttgaaaaaga gatagaggat gaatccgttg
    32941 atatcaaaaa tctcaaagaa aaactggcca aggttggcga gtatattgac gaggaaaacc
    33001 gtggaattcg tgatctttct aataagcggg cccaagttga aagagaggca gacgaggttg
    33061 gctccctaat caataacctt cgaagcaagc ttgccaatat tgatagtatt atcgatggcg
    33121 aggcaaacaa ggtcaaaaac atcaaagatt caagatcaaa ggcctaaaca ttcatagcga
    33181 aacgcaaagc ggaacaggta aaatccttaa gatttttgaa taagaaaaag gagtttgata
    33241 ctttagaggt atcttgatga agtagcagat tgtgaactga caggcatcga tgggaacttg
    33301 gcgtccacgg atgattctgc gactgctgct gcctcttcga gaatcttttc ggattcttcg
    33361 ttgagtgact gatccatgcc aaatgccgat tcgcctgaga aggactccat cattagtccg
    33421 tttagcatct ctgtcatcat gcccaattct cggtctgctt ctggcataaa tttgccaagt
    33481 gaacctttga ggcttctcat cagcccgatt gttggtgcaa ttgtgactac tgtgtctcct
    33541 aggtcgtgga atgttgtcag ccttagctcg atttgctcaa gggctgttct tacgtttcct
    33601 agcatttttg agaccttgcg tacttcggca agttcgtttg ctaaaacctt gctagtgtga
    33661 atatcatgtt tttgagttgc ggcaacgatt ctttggaata gtttttcctc tcgctgtttt
    33721 agtttggtaa gcattccgtc aagcttgctg atttggcttt ggagtttctt tacagcctgc
    33781 tcaattcttg gcttgagtgc tccctttggt ttgattacgt cgttgatctt ctctgatatg
    33841 cttggtgttg gcggctttga ccatgatttc tggaagtgag tcatggctga ttttagctag
    33901 tttattgaaa tcaaaaggtg tgaaatagat ttcacggctc tgtcaaatct gcggtttatt
    33961 ggttgagggc tgatttcact aaaagataga atcacgtgcg gaatacatcc tagtaaaaac
    34021 gaagaaaaat aaaaaacaat tcgggcctag tttattgcgc tagaatgctt tgcggatgca
    34081 tttttttgat gtgtatatct accgcgtgtg tggtagacac attttcacca cataatggac
    34141 attgttccat ttttatagct acaccctcct atagatagta tttaaggctt acgttttttg
    34201 actctggata atcgtcggaa caaaaattag tgtgttgacg atcggaacaa acaaaatttt
    34261 taatagaaaa ctttttggaa atcaagaaaa aaatttttga tattcaaaaa taatttgttt
    34321 tacaaattaa aaatctgaca agaataagtc aaaaataatt tgtttctttc aaactaaatt
    34381 gcaaagaaaa atttgaatcc gtatttacaa aaaatttcag aacaaaaatt tttacaaatt
    34441 caaagacaaa atttttgatc ccgttttgtt tggtttgtag tgtttggttt tttgctgttt
    34501 ccactgtctg aaattagcat tttaggcaca aaatatacgt catgatagat aaattttgtt
    34561 aaatatttat atagagagtc aaaaaatgga ttacacctta tgacacaaac tacacgggtt
    34621 agcgatcgat gcccacgttg cgcaaaggga tcgatgctaa cagatggtag taccggagag
    34681 atgttttgta acacatgtgg gtttgtcgca accgagcgag tagaagagga aggaccagag
    34741 tggagatcat tttcaaaaga agaaggtgaa aatagaacac gaactggaac gcccacttca
    34801 ttggcaatgc acgacatggg ccttgcaaca ataattggcc aagcagataa ggacgcatct
    34861 ggaaaaccgc ttacgtcttc gatgaaaagc acgattgagc gattaagaac ttgggattcg
    34921 cgaagccaag ttcatgagcc agttgacaga aactttagac aggcgttttc ggagcttgac
    34981 agactaaagg acaagcttgc gctttctgat gcagtgattg aaaagactgc atacatttac
    35041 agaaaggcgc tagacaaagg ccttgtcagg ggacgctcaa ttccaggact tgtggcagcg
    35101 gcgttatacg cggcatgcag aaacacagag acgccaagaa cactaactga tgttgcaaat
    35161 ggaatcaata tcaagcgaaa agatgttgca agatgttaca gactgcttct cagagaacta
    35221 gacttgaaaa tgcccgtagt cgacccaatt aagggagtgg caagaattgc aagcattgca
    35281 gaccttagcg aaaaaacaaa gcgaaaggcg ttagaattcc taaaggaggc aagccgaatt
    35341 gaggtctccg ctggaaaaga tccgatgggt cttgcagcag ctgcattata cttggcttgc
    35401 gtgatgcttg gcgaaaacaa gacacaaaaa gacattgccc aggcagcagg cgtgactgag
    35461 gtcaccattc gaaacaggta caaaggtctc aaagaggcac taaaactcta aatctttttt
    35521 tagttttttt tgtaaatttt aatatcaaat agttggtttt gaatctgtga acaaaagaac
    35581 cgtttctggc gcaattgtag tagggattgt aactattttg gctgcacttg ttttaatgcc
    35641 gtcagaaaaa agtgaacgcc aggaaattta tcatgcaaga ctggcagatc ccaaattata
    35701 caccggtggg atttttaccg atacctttgc aatcgaaaaa ggaacgtatc aattcagatt
    35761 tgttccaaat ggggacagtc cccagatttt atcaattgcc ttaaagggag acgccttttc
    35821 gttttctgag gatttcgagc tggagggaac gccacacgag actggcgtct caacttatta
    35881 tacttgggaa tatctaggcc aagacaccat cgaagtatca gaagaccaag agcttcaaat
    35941 tgtaattgac ccgcatggaa atacgcttgg ccctgtttca gtttatctta taaaataaaa
    36001 ggtggcgtga ccccaaacgt agaacgttga gagaacacct ctcactccca tcatttcgcc
    36061 aatgcgttga tggaccacca aactgttcta ctaggtcacg cgtcctcagt cgataggatt
    36121 tatgtccttt cgcattattt tttataggaa tttatgttat ttaagaatta gtgtggatta
    36181 ttatattaaa gatctacata taatgtaata tttttggaca aattttcaaa atcaatatta
    36241 caagacgagc taaaacacat gcaatgaagc ttgaaggtaa agttgcacta attactggtg
    36301 ggagtagagg aatcggaaaa gcaattgcca atctttttgc aaaagagggg gcatctgttg
    36361 tgatatcagc aaaggatccg actagtctac aaaagaccgc aaaagagctt ggagatgttt
    36421 ttgcagtccc agccgatatt agagaggaat ctgaggtgca aaatgtcgta aacaaaataa
    36481 tagaaaagtt tggtaggctg gatattttgg taaacaatgc cggaatctta ccaaaaataa
    36541 agcccctcca tgagattacc gaagatgagt ggaatgaggt aatcgacatt aacctaaatg
    36601 ggcagtttcg ctttacaaag gctgcaattg cacaccttca aaaaaatggt ggttgcataa
    36661 tcaacatagc atcaactgca ggtctcaaat cctatgaaaa tttcagtgcc gatgcatatt
    36721 ctgcatcaaa ggcagcactt gtacttctta caaaatgttg gtctttggag tatgcaaaga
    36781 gtaaggttcg tgtaaactgt atctgccctg gagttatcga aaccgacatg acaaaagagt
    36841 ttcttgctac gccagcatta agggaaatgc tgaattttga gtacccaatt ggcagaattg
    36901 gcacagtaga cgatgttgca aaatcagccc tttattttgc ctcagatgat tcatcttggg
    36961 tgactggtgc tgttttggcc ctagacggtg gcgaatctgc aaaatgaaaa aatcccaaat
    37021 caatttatca tccataggtt gagtcaaagc gttggattct aaaaagaaaa cacgagccaa
    37081 gacgattata cttttagcga taatttggtt tgttgtttca cttccattgc cgtggttttt
    37141 tactgcgcct gatgaggtaa agccacaggt gtttgtattg ttgcaaatag ttggaataat
    37201 atcaattccg tttattatgc tggcaatagt ttggacgata aagccagagc tcaccacgta
    37261 acatgtctgt taagatacca tttacaaatc cgttagacga ggccgcgctt gcagttgatg
    37321 cggcaataaa ggcaagcaaa gcagtcatgg acatctacaa tactacattt agaactgagc
    37381 tcaaagacga tgattctcca ataacgcagg cagacctaga aagcaacgag attataaaag
    37441 aggttttgtc ctcctctggc cttccaattt tgtcagaaga ggacgaagac gatgtgggac
    37501 gattaagcca taaaaaaatc tggatcatag atcctcttga tggtacaagt gattttgtaa
    37561 acaggactgg cgagtttacc attatgattg ggctagtcga aaataaaaag ccaattcttg
    37621 gtgtgatttg cagacctact actaatacta tttttttggc gcaaaaggga aatggtgcac
    37681 acaagcttga aaataactct tggcaaaaac tgacagttag caaaacattg gatcttagaa
    37741 aatgcaaagc agttggcagc cgatttcact tatcagatca agaaaaacaa tttttcaaaa
    37801 accttggtgt atcaagcttt gagagtaggg gcagctcgct aaaggttgcg gagatttgca
    37861 tggggttagc tgacctgtat ctgacaacct caaataagat aaagcagtgg gatacatgcg
    37921 cttcatattg cctcattaca gaagctggtg gaaaaatgac aagcatgaac ggtgctgaga
    37981 ttttgtataa tactgaaaag ataaaccacg aaaatggcct gcttgtaaca aacggcctag
    38041 ttcatgatga aataataaga cgatatcttt cagtcaacta ggttttttga ttttaggaaa
    38101 ttcaaaaggt tttgaacgct ttggtctaat gtttcctttt ctgtatcaag aatgaggtct
    38161 gggttttctg gcgcctcgta tgggtcactt attccagtaa aggctttgat ctcgttgttt
    38221 cgagcctttt tgtacatgcc ttttacatca cgcgtctcgc attcttgcag ggagcatttg
    38281 acatagactt caaagaagcg tttgtcgtcg ccgataattt ttcttgcgtc ctccctgttt
    38341 acgataaatg ggctgactag cgatacgccg catggaacgt tgtgctttag caaaagcttt
    38401 gctagatgcg caactcgctt gttgtgctca attctgccct cctttgaaaa gtcctttggt
    38461 gaaagccatt ctcgcagctc atcaccatcc aaaatagcaa gatttggtac gtgtttttgc
    38521 attgcacgaa ctatggttgt tttgccagaa catggaaggc ccgtcatcca cactacaaat
    38581 gccattttca gtttacctca aaatcaaagt tcttaaagac tttacgacgt aacccagtct
    38641 tgtttttcca gatattctat ttccttaatt atggtctgaa tcttcgtaga gactgccatt
    38701 actgattgaa actgctcgta catttcctgt tcttcttctt ttgagagcac ctcgccttgg
    38761 gcttttttga aaaactcttc ctctttttgt aagtgatcgt ttaggtagat atgataggtt
    38821 cgcaagaatc ttgcaactgg ctctcgcgca tcctcaccgt tttgccattg ctgtaggtgc
    38881 tttacgatgt tttttgcaat ccctctgcta aactcatgct ctatcatgaa tttgcggatt
    38941 tcgtctttta gtgtcccata gcttgcaacg catgcaaaat acgagtcttc ttctctagaa
    39001 taatgaatcg aatctaaaaa ttctgatatt acaaatgtga ttttttgtat gtctggtagt
    39061 ggaatgtttt ttcctgcata caagtccgcg tagcattttt cgataatttt ttcgagtctt
    39121 tttattttta tgtggtcttg ccggagaact tcggttgcgc tcatgtgatc acctttatgg
    39181 attgcaaaca ctgcaagtgc atttcatcat gcccattttc cccatgcatt ggtcatgttt
    39241 ttcttcaaaa cacttgtggc aggtcaccat ggttctgatt caattttaga gactttaatc
    39301 gtttgtactt agagaacaaa gttggagttt gccccatcaa agttaattgt aacaccagaa
    39361 agatacttga ctttgttttg aataattgct tttacaaaat tcccaatctc ctttggctca
    39421 cctagtctcc tcattggcac tgtttttgca agatcgtctg cgtttccaag atctcgtagt
    39481 ctgtctgtgt tgatcaggcc tggcgcaatg tttatgcagc tgatttttct tgcagccaag
    39541 tctttgcttg caagcttgaa gaccgctgtg aatgcaagcc tatatgcgct cgatatgaca
    39601 agccttgggt ttggctccct tatgacgcta gacgttatta caaagatata gccgctgtct
    39661 cgcaccttta gcttttgcag taaaagacaa aatcccaaaa acagctggtt gtggtatttt
    39721 tgccactctt tttccgttac atcccggaat tgttttggcg ggggtcctcc agtattcaaa
    39781 accaaaatgt ctgtctgttt gtgttttgca atgaattttt tgacgcttga taaatttgat
    39841 gtatcaattt cttttcgcga tgtagcaatg acatcgcaat taattgagga aagcgcatct
    39901 gctattgcct ttcctattcc ctttgagccc ccaagtacga ttgccttcat tgctttgttg
    39961 tgacaccgta attgatttta atatactgct ttattcgagt ccaaagagaa ttacgtttct
    40021 tggatcatct tggattatgc caaagtgcgg cttggcaaac cccccactat gatgcccttg
    40081 tggcccagca agcccgaatc ggaataggta gttgtggttt tgctcgattg gaaattcgtg
    40141 ctttcttaga gtatgtgcac caattttttg ggtttcgtgc tggaacgacc agatgtttgt
    40201 gatttggttt ttgtcttgtt ttattgcagc ctcgatgtgt tggtgaccac ataaaattat
    40261 gaaattatca aaatgtgttt ttccttccgc aaatgccgag gccggcttgt agtggtatcc
    40321 gtaatagcta taaaactcgt tgtcctcttc tgaaagcctc tgccaagttc tctggtacag
    40381 ccaaggatca tctccttctt ggatgacttt ttctggcgta attggtccgc catgcacaat
    40441 caaaaacctg tttttttgat ctattacaaa ctggttcccg tacgtgccat ccttgttttg
    40501 tttgaagaac tccaagtctt gttctgataa aagctcgtgg gctttgaggc ttgcatatga
    40561 gctcccattc aggaatcgct tgtacaaaaa cccctcatca tggttaccca tgactgaaat
    40621 catcggatac gattttgaaa gctcggtcat tttttgtagt acctcctttg gggtcgtacc
    40681 tcgttccaga acatcaccca aattgattat tcttgatact gttccatact ggttttggaa
    40741 ttcatctgaa aaaacaatat ctaaaattgt atcaaggccg tcttggtctg cgtggatatc
    40801 agagaaaaca agatccattg gattagagcc tttcttacgt aatataataa tagataaaaa
    40861 atgactatgt tttacaaaaa tattgtatct ttttagcttg ctagcgctac gccgctttca
    40921 ttggcttctt gtttcaactc tattatctga ttattgatat cctcgatttc ctttattagc
    40981 tccgcacgcc tgtctatcaa tgtctttagc attgaggtgt acacgcttac caatgtttga
    41041 tttgatttgc tcatcatgaa gcctttccgg attccctctt aaactcttac taatgaattg
    41101 ttctatcact atagtcaaca aacagctcac cattccaaaa cactagattt accgggctgg
    41161 aaaggagatt ttttctaatg tgccaaattg gccttgggca ctttaaaaac cacagtagta
    41221 caggttaaag gtcaagcgtc tttaggtcgt ccaaatgttt gaggatctgg taagggaggc
    41281 aattagggag ccaacagagg cggcagcatc ttcggttatg caggaaggca taccaagtga
    41341 ggtgcgtggc tttattcctg acctagtaag acagttagat gcaggagtac catcagatgt
    41401 tgtcgcagat caggcattaa acaaaacatt tgatattgtc gaaagcgtag ttctgacaaa
    41461 cgcaacatct catgtgttag acaagttctc ccaacgaata gtagatctgc tttttggaga
    41521 accattccta aacattttgg cgcaggcagc tgcactcata acaaacagca cctctccagt
    41581 ctagtttatt ttgacaaatg cggtacattt acagcccttt acaagacatt ttcctccacc
    41641 gtcatgtaag atagaatcat gaaaacagcc aactgccaca ttacggcact tcattgatta
    41701 acttgggcaa aaccccaatt aatccgtctt acgctagtgc gattttaatt tgtcttgatt
    41761 cgtggttaca gatgcttttg tgtaaggttg cataggattc ctctttgaat gtcagatcgc
    41821 acctgtagca tttccaagca gttgttgtca tattgtagaa atagagtttt tttgtattta
    41881 acgcgcgcgt agcttttgta tggatgattt gtcctgttct ttgttcgtcg gattttggat
    41941 gattttatgg tttgtcgtca ttctgtggat ttcatatgct caaaatttaa gagccgcttt
    42001 taggacgatg agttattgtc aataaccgtg attgggggga aggtttctga aggtcttgca
    42061 aagaggctgg cagaaaaact aaaggctgac tttgtatcgg ctgaacttcg agtctttcct
    42121 gatggggaaa gcaagatcac catatcaaaa aaaccaagaa aaggcaaaat catagttgtc
    42181 aattcgacgt gtccgccagt cgattccaat ctgattcaaa ctctttcact aatctccaag
    42241 gcaagacagt tctcaggaca ggtgatttgc gttatgcctt acattgggta tgcaaggcag
    42301 gaccgggagt ttcttccagg cgaagtagtt acattgtcag taattgccaa gctcctccaa
    42361 tctgctggcg cctcaaaaat tattgtagtt gacatccaca gtaggcttgg cctcaagcat
    42421 tttaagatcc caatcaaaaa cgtttctgca gtatcggagc tggtaaagta cttcaaaaag
    42481 ctgaaactaa aagatccgct tgttgtatca cccgatcttg gcggaatgat cagagcaaaa
    42541 gaatttgcaa ggatgtacgg cactgatttt gttgcactaa aaaaacaacg agataaaaag
    42601 acgggcaagg taagaataat gagttctagt ttggatgggg tcagaggacg tgatttggtg
    42661 ctagttgatg acatggtaag tactggaggt agcatcataa aagcaactga atttctaaaa
    42721 aggcaaaagt gtggacgggt ctttgttgcg tgtacacacg gtctgctaat tgataacgca
    42781 gaaaaaaaga taagaaagtc tggcgtctca cagatagtca gtacaaatac gattcctggc
    42841 aacacctctg ttgtagatgt atctgggata attgcaaagg caatctagat gcctgagagt
    42901 tttttcattg tttcaaaaga gtatctggag cttgcagtag acgagatagt tactttggta
    42961 aaaatgtatg atagattttc caagatcaat gtaatatcaa atctcataat aatacaaacc
    43021 aagactccgt gggaaaagat tgcaagacgt gccacctttg tcaagacatc aggacagctg
    43081 atcagaaaga tgtcaaatgt attctttgat gagaaaaact atgctctgtt gtttggtgca
    43141 aaatcgttta tgtgtaaggc aatcaacctg tcgcaaaaac acatcgatat ttcggaaata
    43201 gaacgctcac ttggcagtat ggtcactacc ttttgcaatg ccaaagtttc acttgagaat
    43261 ccagatatgg taatctatct aatttttaca gatgaagaga atttttttgg tttttcaact
    43321 aggtttgagg catcaagacg gccaaaaaag ccaaccaagt tccaccacga gctagactgg
    43381 aagcttactc gcgcaatgat taatctagca aggctaaacg acgatgaaac tgtctgtgat
    43441 cctttctgcg gtactggaag cactcttttg gaagcagagt cgatgggcat cagatcaatt
    43501 ggaattgatt ttgacaaaaa aatgtgtgag atatcaaaaa aaaatctgcg gttcaacggt
    43561 tttgatgccc aagtctttaa ccaaaattat gactatatgc tgcaaataaa ggacaaattt
    43621 gatgggatag taaccgatct accatatggt acggcatcta aggtctcaga gcccccacaa
    43681 aaactgattt caaattttgt atcaaagatt ccaaagcagg ccaagtttgc aatcatgtgc
    43741 aaaaaaggcc tagacaaaga cattaagcta aaaccaacaa aaaaatatga aatctaccgg
    43801 cacaagagtc taactagggt gattttggta aaatgaagct tgtatttttg ggaacatcgg
    43861 ctgcgcagcc aacagaggcc cgtggtacgt cttgtatttg tcttgaaaaa gacggcgaag
    43921 tcttaatgtt tgatgccgga gagggcgctc aagtatccta tcttaggtca ggccttggct
    43981 ggaacaaaaa aatgaagatc tttgtgacgc acctacatgg agaccattgt attggagttt
    44041 tgggtttact acaaacaatg acactacagc atagaacaga accaattgag atctacggtc
    44101 ctgatgggat tgacgagttt attgcgcaaa acatcaaaat cctaaatttt ggcttgtcct
    44161 ttccagtcat gattactacc ataaagcccg ggttggtttg cgaggaaaaa acctatcttg
    44221 tttttgcaga acatgcagaa cattctgttg tagcattttc ctatctgttc caagaaaaag
    44281 acaggccggg caaatttgat ctacaaaaag caaagagcct tggaattcca gagggtcctc
    44341 tctggcacga tttgcagatg ggcaaagaga tcaaggttgg aaataaaatt gttaagccgt
    44401 ctgaagtact tggagagaaa agaccgggca aaaaaattgg catctcagga gatacgcgcc
    44461 cgacagaaat acttgaagga tttttcaata actgtgatta tctaagcttt gattgtactt
    44521 tctcagacaa gctgaaagac aaggcagttg agacgtatca ctctactgca aaagaggcag
    44581 cattttttgc aaaaaaagca aacgtagcaa atttgatttt gactcatttt tcggccagat
    44641 acaaagacga gtccgagctt ttgcaggaag caaaagaaat tcatggttct gttattgctg
    44701 caaaggacct acttgagata gaaataaagt aaaatgtttg atttagttgc aaaacaaatc
    44761 aaaggggcaa aaaaaatcgt ctttgtgact ggtgctggga tctcacagga aagtgggatt
    44821 ccaacgttta ggggtaagga tggatattgg aaaaaatacg acccgatgca gcttgcaaca
    44881 attgatgcgt tttaccaaaa cccaaagctt gtctgggagt ggtatgaaga gcggagaaaa
    44941 aacatctttg ctgcacagcc aaataaaggg catttggcaa tcgccgaact tgctgctcat
    45001 aaagatgtaa tagttctgac acaaaacatt gatggtctgc atcaaaaggc cggaagcaag
    45061 aaagtctttg agcttcacgg gagcataatt agaatcaagt gcactacctg cgaccacata
    45121 gataatgtta ctaccagttt tgattttctg cctccaaaat gcaaatgtgg aaacatgcta
    45181 agaccagatg ttgtttggtt tggcgagccg ctacctcagg atgtgtggat aaacgcaatc
    45241 atgcatgccc aaagctgcga tgtaatggta attgcaggaa cgtccctagt cgtatcgcct
    45301 gcgaacacac ttcctttgta tgcaaagcaa aacggtgctg tcctaattga ggtaaatccg
    45361 gaacgaacct tgatgtcaga agacgtgaat ttatcattac gagaaacatc agctaatgca
    45421 ctgccgcatc ttgtgtcatt atttgatagg acttgaaatt ttaattattg agtgaatacg
    45481 ccgtatatcg tcgcatcatc gcttccctgt ggattaaaca cggatgatga tacggttcca
    45541 tagtctgcgt gttctactat catttctact atgtgaggac tagtcgatac atcgtttaca
    45601 ctggtatcaa attgcgatgc aaagaattcg ccagaaaaca gctccttttc agagtttcgt
    45661 agcgtgacag taactttgag tgtttttcct gaggtgtctt catagtggat tgatactgtg
    45721 ttgtcatcat ttctcattgt tgtgagctgc agcgtttcaa aaatttgtgt ttgaggagta
    45781 ggatctactg aaattgtttg ggtgtttgtt tgaggattag ttacagttac ttggactgat
    45841 tgacgttttt catattggca gtctggcagt gtgattgtgt gggtaagctc ttttacacca
    45901 tttactgtct ttaccatttt aggaacagaa atcttgcagt tgttttcgtc atctttttca
    45961 taaacttcgc cgtaatagac ttctttttgt ggttgtggaa cggttatttt ttcctgaatt
    46021 ttcttaattg gatttatctc gtctgggtct ggtgcaattt gttcaatttt atctccaaca
    46081 ctgtcaactg ttttatcaaa ttcgtagttt actctctgaa cagtagtatc cttaagatca
    46141 cctaggtctt gtgccaccgc atcaaggcca gaagtatgag tagaagacag cttgccaatc
    46201 tccggataga gcattattcc tccaattacc agtattacgc ctactatggc aatctttagt
    46261 atcaactacc taaggctttt gtaaaaatca taaaagtcta ggaattcatt ttcgctgata
    46321 aattaggaaa aaaagctgac taattttgtc cagtaagcca ctcgattatg cgctcgatgt
    46381 tctttgagtg tattactacc ttgattggcc ctagcgggga ctcgttatca tgctcgcata
    46441 acgatataaa gttggcaaat gcagcctgtt tgttaagata aaaccacgtc gaattttctt
    46501 ccaagttgtt gttaaggaat cgaaaataga cacgttgtga tttgtggttt tggatagatt
    46561 catgaatttt cgatagtgat gatatgtcct ttgagtttgc cttaattgta tcgttggtag
    46621 ttttgatttc tatgttatca aagacattag aaattacttg ttcaagtttt tgtggatctt
    46681 ctgaagggtt tatctcgcaa aacgcttcta ttttacaatc aactactggg atcatttgag
    46741 ccatttttga attatttcgt atgctgtgtc aatcagctga tcaattgtaa gattattgtt
    46801 tgagatggtc tcatcagcta gtgcaattga tgcactgata cctacactga tttctcggtt
    46861 gtcgcgctca tcaaatgtat tacggtcttg tggatcatca gttctcttac gcgtagtcaa
    46921 gaaattgtac cgtgtattag ttgatgcatg aattgacaaa agttttactg tcgcaatttt
    46981 ttttaggact tcaatttctg tgtttgagcg aacaccatca attaccacaa cgtcagattc
    47041 agagtttatg atttggtctt tgatcagctc agctactgcc ccctgtccgt ttttttgccg
    47101 gagatcaagc attagcttgc caagattttc tccggttggc gctaaattcc tttttttagc
    47161 ttctgcccgt accgcatcgc ccatattgat acttgtaaat cccttttttt gtagaccggc
    47221 agctattgtg gatttaccag ctcctggcat tccagtaaga cagacgataa gttttggcaa
    47281 ttaggcacac tttttgtatc tattctatga atttaccggc ttctgtataa tctacgagac
    47341 caatttaccg gaatccatta aaaacgtaaa aacaaaaacc cgtaatgcga acatttgttg
    47401 cagtggagat atcaaatcag cagatattgg attcaatcaa aaggctacaa tccagcctca
    47461 aaattgaagc aaagccagtt gatacacaaa acatgcactt tacattgatg tttttaggtg
    47521 aagtatctga cgagatggca caaaaaattg gggctcagct aaaaacaata gagttttctc
    47581 aatttaatat gagttttgaa gggattggtg cctttccaaa gcccaaattc ccacgggtag
    47641 tgtgggttgg actcgacaaa gagggcggat cgatgctctc atcgcttgca aaaaaagtag
    47701 aagaaaagct agccccactt gggttttcta gtgataaacc gttcaagcca cacgcgacaa
    47761 tatttaggat taaaaataaa gttgaggata tttcagatga gttggtaaaa tattcatcta
    47821 caaagctcgg aatacaaaaa gtaagtgagc tcaaattcaa aaagagtgtc cttacccctt
    47881 caggtccagt ctattccgat ttagaggtga tactggcaaa atgatcgaga tcctaaaacg
    47941 tgcagaagag cttgttacac cttctgaaaa attacataaa caaaaagatt ctattgtcga
    48001 acaagtaatc aggcttgttc aaaaacaaat atcgtcttat ccccagataa ctggcattga
    48061 gatagttggt tcttttgcaa agggaacttg gttgccagac aaggcagata tcgatatttt
    48121 cataaaattc cagacaagtg tttcagaaaa agaattttct gagcttggaa aaaaaattgg
    48181 gtttgcgaca cttaaggact acaacccata tgtaagatac gcccaacatc catttgttga
    48241 agcagaaatc aaaggtacca aggtaaatct ggttccttgt tatgatgttc aagaaggtgg
    48301 ctggaaaagc gcagcagaca ggtcaccatt tcacaccaga tttatgataa actctctgac
    48361 agctcagatg aaaaacgagg tgcgactgct caaggcattt cttagggcta acgatattta
    48421 cggcgccgag attgcaaggc agggcctttc gggttatgta gcagaggtat tggtcctcaa
    48481 ctttggttca tttgcaggcg taatcaaggc aatctcagag ctaaaacaag gccagataat
    48541 tggtaaagca gcaaaggagt ttgatactgc cgttgtgatc atggatccaa ttgatagtaa
    48601 cagaaacctc ggtgctgcaa tatctacaga aagtgtaggt aaattcgttc tcgcatcaag
    48661 ggcatttttg aaaaagcctt caatttcgtt tttcaaacca aagaaaaaac ctcaaagaaa
    48721 gcctaatgat ttacgcaaaa atgttcttgt gttgtcattt aggtacaaac aaaggagtcc
    48781 agacataatt tgggggcagc taaaaagagc tgctacgtcc attgcaacac aaatagaaga
    48841 ggaaggattt tccgttttga gaaaaacagc tataatatca gaagacgcta atgcggtgtt
    48901 actgtttttg cttcaatcaa caaagcttga acaaaatctt ctcagagttg gaccggattt
    48961 ctacactgta gaacatgtgg agaaattcat ctcatcaaac aaaaagaaaa gtgttacaat
    49021 gtggttaaat gaacaaggaa agatttgctc catacaaaaa agaccgcaga ctgatgtaat
    49081 gttgtttttg aaagatttga taagaaagaa tttggatcgg gtcggcgtgc cggcaggtct
    49141 caaagatgac atcaaggcat tcaagcttgt cttaggtcac aaagtaacaa gcaaatccat
    49201 taaagaggcg atctcagagc ttgtctcaac agatgagaca attttttcca gttgatattt
    49261 tagccaagga accatattct atagttcttg gatatccaaa agccagtaga cgacagctca
    49321 aatcaagact agctgagctc aaaaagcttg gaattgaaga tgtgtcattt caaggaccta
    49381 ttcttattgg caagattgca gttttaggaa agggctacac aggtgttgta attctggcaa
    49441 aaaaaggtgc aaaacgagtt gcactcaaga ttcgtagaat agattcgcct cgtgaatcca
    49501 tggcaaagga gactatacta cttgaggctg caaacaaggc caaagtaggt cctagactga
    49561 ttgaaagtag cgacaatttt gttgtaatgg agtatctaga tggaaaaaag atctacgatt
    49621 ggatcaatga gcttaaagga cgtggaaggg ctacgagatt aaaatctgta ataaaaaaag
    49681 ttcttgagga ttgccacagt cttgatagag ctggcctaga tcatggtgag cttagcaaca
    49741 tcacaaaaca cgtcattgtg ggaaaatcag tcacaataat tgattttgaa agctctagcc
    49801 ttgagcgcag ggtttcaaat gtaacttccg caacacaagc agttcttatt ggttctggcc
    49861 ttgcaaaaat ggttcgacgg atttacagcg ctccatcaaa acaaaagatc atcaagtcat
    49921 tacgacaata caaagaagac caaacgcagg aaagttttga taaagttctt gaggttctaa
    49981 ggttatagta tgtcaaagca ggcagtaaag tttctaaaaa aggatccaaa gcttgcaaaa
    50041 ataatcaagt atgttggaag ctatgaaatc agccttatga aaaacccgta cgaatcgttg
    50101 gttgaagcaa ttattactca acagctctca ggaaaagcag cagaatccat ttcaaaaaga
    50161 tttagaaaat tatttgtcag atttccaaaa ccagcagatg tgatcaaaat gcccgattcc
    50221 aagattcgtt tagctgggct gtcaggcatg aaggtaaaat acattaagga tttgtcagaa
    50281 aaaatcgaat caaaagagct gaagatgcgt tcgctaaaaa acatgacaga tgaggaagtg
    50341 atcatgcagc ttacccaagt caagggggtt ggccgctgga ctgcagaaat gtttttgatc
    50401 ttttctctag gtagattgga tgttttgccc gtaggggatt tgggtctcaa aaaggggata
    50461 cagaggatgt attccatgcc tgatctccca gaaaaggacg aaattgaaga tctggctgaa
    50521 aaatggaggc cctacaggac agttgctacg tggtatattt ggaagagcct caatcagttt
    50581 gataaaattg gatgaattgg gagaactttt tattattatg aaaacaatta atgcgttcta
    50641 gattgcaaaa cactcttgtc accgtatttt cttgtctagt tgtaatttca attgtatcat
    50701 ttgttttgca ggatcaattt gctaatgccc aagaaattcg aattccaaat tggataaaaa
    50761 gtcaaatcaa gtcttgggca gacagacaag cttcagatgg cgactttgta gagttacttg
    50821 gcgagctaaa caagcaaggt ctgattaaaa ctcacgatat gaataaaaac caaaacatgt
    50881 atcttttacc aaaatatggg caaaccattt ttgtaaaaat tgcaggaagg actggcgatt
    50941 ttgaacaaac aagcccagtt actctgattg ttgtagaccc ccaaggccaa agatcagagt
    51001 acaccatact ggttctcgaa agcagtgttt attctactgt agtaccaatt agtcatgatt
    51061 caccaattgg cacatacacc gtgtttgctt atcataccaa caaggagctg cctacatcat
    51121 atttttttgt aaaagctgat ccacagattc cttggtggat caaagatgta gccaaatggt
    51181 tggctgcaga taaaatctct gacagagatt ttattttatc aatgcagtat ttgattgata
    51241 agaaaataat tgatcttggt tttatagcta gcaccaacca agactcgttt cttgatgtga
    51301 gtgtggacgg tttgaaggca gtcaggcgtg gaacgatgca gtctttggat gtgcatgtta
    51361 gcaatatggc tggccctgtg gacggtgcca tggtttttgt gcgcgtagag gattatggtg
    51421 aaaacatact tgaagaattt actggtgtaa ccgattctag tggaaattac aatctatcgt
    51481 gggaattaag tatggacgaa ttcaatgaca ttaagacctt gcttgtgtat gttgatgtaa
    51541 cgaatggagt gtcttcaaag acaaaactgt tcacatttga ggtgtattgt ctttgtggtg
    51601 aaccaaactg caagtgtcgt aactaaagta ttggtttgtt aaaggatttt tctgtgtttg
    51661 tataatgcgt atgtaactat ccagtaacct gcataccaca acaaattaac aggatgttcc
    51721 aaagtgtatc ctgcatttgc ttccaggtta tagtagatga catcagctac tgaaaaagct
    51781 atgacgccaa ataacaaaag aagccaggcc tttcccataa ttccttctct gaaaatgctt
    51841 gcaccgtaaa tggtcatggt aagtgaaaca gatgaaacga tgatgtaata caatgaaaga
    51901 aggaacaacc cgtctcgttc aaaaccccat accagattca aatagaaaag aaccagtgaa
    51961 atgggtagta tcatcatcca tagaagtgtt tttgctctaa tctttggttt gaaaaatgaa
    52021 acatttagga aaaggtgtcc tagcacaagt gggtaaaacg gaataaaaag aatgtcagcg
    52081 ggggatggaa aggctggctc ttctaagaca taatcgtaca gaaaatagag tgtttctcct
    52141 cctgctgcac aaaaatacgc tattgccaaa aatagataag atttgaagaa aatttttgtt
    52201 gatcggtatc ttatcgcaat aataaacccg gcagcagttg ttataattgg atttaaaatt
    52261 gaaaaagcgt atgacgccaa atcgctaata tccccttgga gcacgtaaat ggatgcataa
    52321 tgaaatacgg tcactgaaac gacgatcagc atgattatct tcagatttat tgggctgtct
    52381 aatttctcag gcacaacctc agtttcgttt tttcccaatg tggtgcttat gaattcatct
    52441 tttaagtaat ttttgaattt agtgggaccg gtgggacttg aacccacgac ctctagcacc
    52501 ccaggctagc atcataccaa gctagacaac ggtccctcat ggtgaaaaca aattgtgaat
    52561 ttattaaatc gttgtatcta taatgaagtg tgaagatctg ctcaatttgt cacaagattt
    52621 ctgcaacaga ccaagaccac acagactgca aagaaaagct tagagtggag ctagaggcag
    52681 aagatttcaa aaaaggtatc cctgagaaat tggacatggt gaaaaatgca aacgacattg
    52741 gcccagatat taaggcactc ttaggtcaca tatccagaga aaagaaaaag gaatcatagc
    52801 catttttgca ggcgttctcg ttcaaatttt tccttttcta acatgtgttc ttctgctggt
    52861 ttgtctagtc gtgttattgg atttggttct gcaaacatgt ctacatatac atagccagca
    52921 gtttccccca cctcaacaaa acatccacct tttccatcaa aaattgagtc agttttttcc
    52981 tttttaattt gtgagattat atcgtgagct actgccaccc cttcaccctc tgcaaaaatt
    53041 cctgcttttg gcactgcagt gttagatgta actaacatgt ttgttacatc accaattgca
    53101 taaacaccat cgtgttgagt cttgcagtcg cgctttacag aaatgaattg gccttcttgc
    53161 gcaaaacctg catcatacac taccttgggt gccttatgag gtggaactgc aatcagcaga
    53221 tcaaattttg taaaattccc attttcaaat tttaatgtat tttgttcaac tgaaattgtt
    53281 ttgcaagagc catggaattg gatgttctca gattctaaca tctctaaaag ctgcttgctc
    53341 acctctggcc ctgcagccgg aagtgtgatt ggcgcaagac tgtaaaagtc aattgtaatt
    53401 gcattccgtt ttccaagctc ctttaacatt gcattgatta tcaatgcagc ctcaaatgga
    53461 gctggcgggc atttgtatgg catcccagta atagcaaaga cgagccttcc tgacttcata
    53521 tcaagtattc tttgtcgaat tttcggtacg tgctcaatat catatagaat tgttccgtat
    53581 gtggcaaggt ttgagatttt ttctggggct agctcaactc ccactgcaat tatcagatag
    53641 tcgtaagaga attcttttgt tgttgtcttg acaattctct gttgcgggtc aattacggtg
    53701 actggctcat ttacaaattc aattcccttt tttgtaatgg attgaagagg tttttttgag
    53761 gtctcaaact gccttgttcc ctttatgatc caaagcttaa ctagatccat catgaagtag
    53821 tcttttttgt caattatggt aattctcaca tcagatgaaa ggtttgatcg aagctcattt
    53881 gcagctgcta atcccccaaa acccccacct aagattagaa tttgtttttg caacttagcg
    53941 ttcttgtgtc gttggtctaa tcaaaatctc attcacattg acgtgtggtg gtgattctac
    54001 tgcgtatact atcgcatttg caatgtcttc tgcctttaat gcctccatct tttttgcaga
    54061 ttctacaaat gccttaagtg agtcatctgt gatggtattg gttagctcag ttgcaacaac
    54121 tcctggctca acacatgtga cgcgtatgtt agatcgctgg ctgaattcct gtcgcagtcc
    54181 ttcacttagg gcaacaattg catgctttgt tgcgcaatac acagagcctg caggaaagac
    54241 aatacgtcct gcaacagatg aaatattgac tatgtggcct gattttttgg caagcatatg
    54301 aggaattgtc gctgcagtac agtacaagac tccctttatg ttcacatcaa tcatttgatc
    54361 ccattcgctt accttgagat ttttgaaaaa gcttagtggc atgaggcctg cgttgtttat
    54421 caaaatgtct actgtgcccc attttttcac aactgtatca acaaaggagt cgcagtcaga
    54481 tttttttgtc acgtctaatt tttgggcaaa aacatcgcca ccgttttttg caatttggct
    54541 ctctagttcc tctagtctat cagatcgtcg cgcaccaatt gctatctttg cgccagcatt
    54601 tgagagtgct atggcggttg cctttcctat tccactgctt gcgcctgtga taattgcaac
    54661 cttgtcttta atcattctag tcagtgtggt ttgaatggtt gttttatgtt tttgcggctc
    54721 tgccaaaccc atcaacatag atttggcaag caataattta caatttatta gctgtgaaat
    54781 ctaagaataa ttagtgaaaa tagtaaactg tacatattgc ggcgatgaaa cagatcttcc
    54841 gtttgagtgt aattactgca aggatcagtt ttgtgccgaa caccgattgc cagaagaaca
    54901 tcgatgtgtc aagcttacat cgatccgtgc aaaaagattt ggcgaaaaaa aagtaatcag
    54961 aagacaaaaa cgtggcttct tagacaaagt ctttggtaag cacaaagact agtagattga
    55021 tttacctggc gttagctttg aaatccttgc ttggtaaaat aaagccacag caagtgcaaa
    55081 caatacaagc gcagtaagcc aatgcgccgt aatcagtgtg acatatgcta ttccaaatcc
    55141 aactgagaca attccctgag atacaagctt aatccagttt gcaagtttga ccaccctgct
    55201 aataatctct atgacaaata atcccacaac cggataaatt gtcagcaaca gaaaatctat
    55261 aacatctaca tcaaggtggg acatcttttc tttattttac agtcactata atttagtctt
    55321 ccaaatgcag cttgattgag atgttttttg caatcaatgc ctgagcgttc tcgtttggaa
    55381 ttgtggcaac atcctctgcc ttaaatggcc catatctagt aaagtccgaa cccacaaact
    55441 gatcaaagtc ccttaagaat cggacggtaa ttggttttgt cttgtgtttt tttgacacag
    55501 actctaatag ctttgttctg ccgttgagtg ttgcagaaag tatcatttcc tttctttctt
    55561 ggagttgttg ttgagactcg actatgaatt tttcctcatc tagtaaattt gtataatcaa
    55621 gatcgtcttg ggttgtcacc ttttcaattc ttgttttcaa gaggagaaaa gtgatctgtg
    55681 ttatcatttc cacaagtctg ttttttatct tgttttctag tccgtcatat ccttcacttt
    55741 ttagcttgcc gagatattca gagagtgtct tatagaattc cggatcaatt tcctgtacct
    55801 gatcattttc gatttctcga agtagaactg aatacaacga gtttacatca attttttttg
    55861 tttctgacat cacatacctt aatccttaaa tgttgcggtt tttcgtgtaa aaatgaagga
    55921 atcaaaatgc catataaagt aactcatggt gaagcaacac aaacaaaata ttcagcggac
    55981 gaagtttttg caaaaataaa acacgaaaac attcgtttta tcgatttaca gtttacgagt
    56041 cttgttggga gataccacca cacgacgatc tccgcagcac tttttacgcc cgatcagatg
    56101 aaagacggcc tccccaagct ggacggctct tcaattgttg gttttacaaa tgtcgacgat
    56161 tccgatctta tcttaaagcc ggatcctaac acgtttgcta tcatcccatg gatgacagag
    56221 cataaaacag cacgattaat ttgtgatatc tattggggcc gtggaagagg taggctagag
    56281 cgagacccta ggggaattgc acaaagagca gaggaacatc taaagacaca gggatttgat
    56341 tttagttatt ggggtccaga agtagagttc tttgtttttg acaagatagt ttgggatgtc
    56401 ctgaccccat acaaaggcca gtcttactcc attgaatcaa aggaggcacc atggagtcaa
    56461 gaaggaaccg gttatccgat gggattgcag gaaggatact atccaagcac gccatctgac
    56521 actttgactg cctttagaaa cgaatgcgtt gatgtgctaa atgagaactt tgggatttta
    56581 tgtgataatc atcaccacga ggttgcaact gcgggccagt gtgagattga tattagattt
    56641 gattatttga caaatgccgc agacggtgca caaagctaca agtatgttgt cagaaacact
    56701 gctaaaaaac acggaaaggt tgcgacgatg atgccaaagc cgatttcaat ggatgctggt
    56761 tctgggatgc actgtaatgt tagtttatgg aaggatgcca agaatgcgtt ttttgacaaa
    56821 gacgccaaag atgagataag ccaagttggt agatattttt gtggcggtat gatgactcat
    56881 gcaagagcat tgtgtgcaat tacaaacccg acaacaaact cgtatcacag attggtgcca
    56941 ggatatgaag cgccagtcta cattgcttgg agtccaagca acagatctgc tgctgttcgc
    57001 gtaccagaac atttcagagg ggaaaagtac gcgtatctta agagatttga gtacagggtg
    57061 ccggatcctt cctcaaaccc ctacctggtg tttcctgctc ttttggcagc tggtcttgat
    57121 ggcataaaga aaaagatgga tccgggtgac ccagtcttag aagacattta caagatgaca
    57181 aaagaagagc gtgcaaagcg tggaatcaaa aacgttccag ctaatttggg cgaggcatta
    57241 gatgagcttg aaagtgaccg caaattcctc aatccaattt tctcaaatga tgtaattgac
    57301 aaaataatag aacttgaacg aaaagatcac agagagatct caattagacc ccatccacac
    57361 gagttctatc tttatttcga tgtctaggct agcggtcttc cagtaagctg caaaatatag
    57421 aaaagcgata tggcagtcag accaaaccca attccaagat aaattccgcg tcttttgtta
    57481 gatgttgctg ccttgtatac aagaactgtc ccagcaagtc ctacaaccag aataaaaatt
    57541 ccaagaaaca tatagtcgag tgtgccgccc tgaataaacg ggtagaaaac acctgagaga
    57601 agggcaaaga atgctgcaag ataggcgtat tttacacttg tgattacctt ggtaatacca
    57661 gccattggtt ttggtaaata attttagcaa ataaacgttt gagagtgatt ttggccgaaa
    57721 ctacatttcg cccatgccca tatcgcccat tccacccata cctcccatgc cacccattcc
    57781 cggcatacct cccattcctg gcatcccacc ttctgggcca cctggagaag cagatttggc
    57841 gacggcaatt acatcatcaa ttcttaaaat catacaagct gcttctgttg ccgcagaaac
    57901 gatctgattc tttactgcca gtggctctac aatatcgctt gttttcatgt tgcttatttt
    57961 tgctttcatc acgtctattc ctgtccattt ttctcctttg agctgtcttg agcgtaagtt
    58021 agttagagtg tcaattggat ccatgccagc actttctgcc agtgttaatg ggattacttc
    58081 aagcgagtct gcaaattttt ctgccgcaag ctgttcacga ccttcaaggg attttgccca
    58141 agttctgatt ttagttgcag catatgtctc tggagcccca ccgccagcaa cgattgccgg
    58201 tcgctccatt acatctttta ctaccataat tgcgtcatga actgatcttt ctacttcgtc
    58261 aacaacgcgt tgtgatcctc ctcttaagag cagggtgaca gattttggat ttttgcatcc
    58321 ttcaatgaac acccatctat cttcttcgat ttttctttcc tcgactagtt ctgcagcacc
    58381 aagatctttt tcaaacagat catcaaggtt tgtgacaatt cttgcgccag ttgctttggc
    58441 aagctttgcc atatcacttt cttttacacg ccttactgcc aaaattcctg ccctagccaa
    58501 atagtgttgt gccatgtcat caattccttt ttgacacaaa agcacgtttg caccagaagc
    58561 aattactttg tctaccatgt ttttgagcat tctgttttcc tcatcaagaa atgttttcat
    58621 ctgttgaggg tttgagatgt ttatctttgc atcaaattca gttttgtcta tttctaatgc
    58681 cgtgttaatt aacgcaatct ttgcctcggc aatttttttt ggcattccgc catgaactac
    58741 ctctttgtca agtactatac cttcaatcag agtcgagtct ttaatggagc ctcctgcttt
    58801 cttttcaact ttgacatcat cttgatcaac tatgaatttt tcaccgtttc tttcagcaac
    58861 agcaagaact gcttttacaa caagcgtagc taacaattcg ccaccctttc ttacaagttt
    58921 tgtttccatt gaggtacttg caattttatt aagaaccgat ttgtcgttgg gagtaacatc
    58981 ctctgaaatt tccttaagat attgaattgc ttttttggag gcctttctat aaccgtccac
    59041 aattattgta ggatgaacat cttgggttag aagagattca gcattttcta acaacgcccc
    59101 agcaagtact actgcagatg ttgtcccgtc accaacctca ttgtctgtag tctttgagat
    59161 ttcaactagc atttttgctg cagggtgttg aacgtcaatt tcctttagga ttgttgcgcc
    59221 atcgtttgta attgtaacat cgcccaaaga atcaactagc atcttgtcca tgccgcgtgg
    59281 gccaagacta gtatgaacaa gttcggcaat aattttagct gccataatgt tgtttttttg
    59341 ggcttctctg cctttgtttt ctgaagagcc ttcttttagt atgacgactg gaagtgaacc
    59401 gccccttgca gcttgtaaac tcataacgaa gagaatttta tttttccctt tttatacttt
    59461 gtttgctgaa cagtgtgtgc caaaagaaac cactaaactg gacaatgttt gttttttatt
    59521 gttggtttgg agttttactg tttttcttag tgcgatctat gaagaaaatt ttcgccttta
    59581 aaccgaataa tattgtttta aattgggaca ttcaagtgcc agattatgac aaaatccacg
    59641 tacaaaacag cctacaaaaa aattctagca gatttacaag aacacagtag gtggtttgaa
    59701 aactcgattc ccctaattgc gagtgaaaat gtgccgagtc cggctgttcg cgaggcattg
    59761 acatctgatt ttggtaaccg ttatgcagaa ggctggccag gcgagcgagt atacgcaggc
    59821 tgcatcttca ttgacaaagt agaaattcag tgcatggatc ttgcaaaaaa actctacaga
    59881 gccgagtttg cagatgtaag gccaatttca ggtgttgtag ctaatttggt agtttattct
    59941 gcatttacaa atccaggtga tgtgatgatt gcgccatcaa ttccagctgg tgggcacatc
    60001 tcacatggta aaaaagaaca ttctggaact gcaggccttg tccatggcct tgatgtagaa
    60061 ttttatccat ttgatgccga cgagatgaca atcgatgttg acaaaacaaa agaaaagatt
    60121 gaagagttga aaaaagcaaa cagacttccc aaaatggcaa tgtttggagg atcattgttt
    60181 ttgtttccac atccagtaaa ggagcttgca gattttctca aggcttacaa catccacatc
    60241 aactatgacg cagcccacgt tgcgggccta attgcaggtg gacaattcca agatcctcta
    60301 agagagggtg cggacaccat gacgatgagt actcataaga cattgtttgg cccccaaggt
    60361 ggtttagtat tgtcatatga aaaatttgga gaggcaataa aaaaggcaac cttcccaggc
    60421 ctcacaagta gccaccacat tcaccacatg gcggcaaaag caattacgtt tgcagaggca
    60481 ttagagtttg gcaaagatta tgcaaagcaa gttatcaaga atgcaaaggc gcttgcagtt
    60541 gcccttagtg aaaacggttt caaagtgtta ggagaaaaac ggggttttac aaaatcacac
    60601 caaattgcag taaatgtttt agattattcc gatggtggca aagtcgaagc agaattagaa
    60661 aaggcaaaca tcattgtaaa tcgtcagcta atcccaggcg atatcaaggc aggaagaaac
    60721 tattttcatc cgggtggaat caggcttggt gtagctgaga tcacaaggct tggcatgaaa
    60781 gaatcagaaa tgaaagagat tgccgcgttt atcaaaaaaa tcgttattga taaaaaagat
    60841 ccaaaaaaga tgacgccaaa ggtcaaggct ttcagaaagg attaccaaaa ggtccgctac
    60901 tgttttgata acaagcttgg tgcctacgag tacgtcaagt taagatagtt attggtaatc
    60961 agtaagtaaa agctgatcgc cttttctttt tccaaacacc agatcgattt cgtctgccag
    61021 tgtagcaatc ctacctttga catatggacc aacttcatat ttttcaacga gtcttttagc
    61081 aagatgtagg tatttttcaa ttgatggtcg tgtaattgtc tggataagat catttccaca
    61141 aatacaatgt ttcaaaagtg gtgttcttct gtattttctt ccacatgaag tacaccggaa
    61201 tttttgtttt ccatatgctc gtaggttccc aattatgtcc ggtactagat gtgtggtgat
    61261 tacatttgtt acaatttcat ttgtgtccac tgcgtttatc agctcagctg ttcgaatctg
    61321 catgtcaaat ttgtcaagca ttgagccaag tgtcgaatat gcactcctag atctagaggt
    61381 agtaagtgtt gttgttatgt gagtaaaata atatccataa aattgcgctt ctgtttctag
    61441 tcgtgatttg attatctcaa ctgatgttac ttcggttgcc tttctaccca gtaatgtatc
    61501 ctcaaaaaat tctaatggca gtatctttgt aacctccaag ttgtgtgctt gtggttgtga
    61561 ttcgtgcggc aaaacggttg gttggaccaa aagtggcgca tccattagtc ctccaatttt
    61621 atctgataga aactgtcgtg aaaaattgag tagtgcatcc ataagcaaca taattgagtc
    61681 tgcatccccg tctgcatcac gtcgtttagc agaatgccag ttcggagttg caaaacagac
    61741 gtgtgtgtta gtatatccaa ttattcgacc gacaatcccc acagatgtgt gtggtgcaag
    61801 gccaattacc agttgaccga ttaattcatc agtgtttctg acattataga acggcatctt
    61861 tccgtagagc ttttcaagtt ctgtgtcgat atatttgcaa acattaacca gatacctacc
    61921 actttcataa ggaataacta catcttgcat cttgatttcg acaatttgat cagggtttgt
    61981 tagtggtttt ccgtcaatgt cttctgtata tcctagctca tagagccttt caaccgtagt
    62041 tccaatccac gacggtttga agtgtgttag tggtgagttt gttgcatcga atctgatcgt
    62101 tccatctttg aataccgtta gatcaaggct ttgtcgtatg agtccttttt caagtggttc
    62161 tgctattctg tcttggctta tcagctccat cacacctttg aatggttctt gtacccgtat
    62221 gcccgtcttt tcctgtattg caaggatttt ttcttttaat ggaaagggtt tgaatgagtg
    62281 tgttggtact ttctttttgc atttttggca aaatggctgc tcaaggcttg ccctgcagat
    62341 agtgcataca aactcaattg ttgttttgtt accacatttt tggcagttta tgctaattga
    62401 tggttcgttg cagtttttgc atatacggtt gtagatgttg cagtaaaagt tagggctcga
    62461 gcttgctttt agaaggtctc gtgtgctccc acccttatca ccaacgggaa atagtgtgtg
    62521 tactggtggc ttcattagtc gtggagcggc cttctccggc cttccaatcc ttacaccgat
    62581 tgaggttgaa aacttgggcc tgattattat tgcggaagat tttgtgatta tttgtggaac
    62641 tgtatcagca tcatcaagct tgatttggtt tgcaaataat aattggtaga aaatttgcgc
    62701 ttctgtttct tgtagtatta attcgtcact gacaacctcg tgtgggacgc caagattttc
    62761 caagattttc ttgcagttta atgaatagct gatagtatcg tcttgtattt tgattggctg
    62821 caaaagttgt cttagctcca taaccgagat ttgctcccaa taaaacaaat acttgggatg
    62881 aagcggtatt tggaagttta gtgacagctc taaggcctcc tcaaagtttg gagttctgat
    62941 cagaaactgc tgcagttctt gcgagtttgg ctcgtatttg agaatttttg attttagctc
    63001 ctcaatccaa aactcctcta catatccagt tgggatgagc tgtgcattat tttctaaaaa
    63061 atctccaaat gagatcagaa tgtctccaag atagaggatt ttttcgattt gatctttgat
    63121 tttaatccca tgttcgacat tttgaatttt tactacgttt ccatttttga gtcgaactgt
    63181 tggtgtttca attgaatcaa caaacgcgat ggttgcccct tttcctggaa cgtcaagctt
    63241 tacttgcgtg ccaacagcaa tggtgtgatt tagaatttcc gcaacaactg gatgaattcc
    63301 aactgcggca aaacctgtat tacaagatct cccatatcga agccgaaatc caccaatctt
    63361 gtttggaagt gaaagaactg atcttcctgc aataacctct tttagtcttc gtgcagcaga
    63421 gtccgcctcg tcgccttttt gtattccgcc ttttagttcg ctgagccatt cccaaccatc
    63481 cagtccatat agttcgattc gtttaagcag tttttttgct cgcccaatta tgccgtcatt
    63541 tagcacgcgt aatgcacccc cccgaacgcg gtcggtcttg attcgggcca tgtttttgtg
    63601 atttactacc tcgtttgggt ctgtgtccac accgtcaagc tcaactggca ggtttgagat
    63661 tacagtaaca acgtcctcat ctaaaacatg gtattgaaaa tttcctacat cccgctcata
    63721 gactcgaagc tcttcaacaa acctgcctgt ttcgtcatca aacgcattgg cctcatattt
    63781 tccgagtccg actaccttac gtacgtgatc tgcaataagc atggtcactg cagattcggt
    63841 tcctcctgca gaacgcattg gtcctgcgat ggaaacagat aggtattcag aaccatccct
    63901 atttttcttg attgtaacag ctgagattcc ctgtaatggt gcaatcgtga ctccttcggt
    63961 tactattgca agccctaccc gaactgcatg atcaagtcgt tgctcaagcg gtgtttctgg
    64021 tggaagatat tgcccaagtg ctatgctttc tgacagatct agtgcggcaa gctcctttcc
    64081 ctttgtcttt aagagggctc ttagcggttc agcaatgtca atatcgtgca tttttgcaac
    64141 cctatctgca agatcaaacg cgatttttgg ctctacgttt ccagaacaat cagtaagtgt
    64201 tgattttgcc tcagctgctt tctcaaaagt ctggtatgtt ttttccgaaa tttggctgta
    64261 ataatccaaa tagtagttgg gcatttgaac tcccttgata cgggatattg ccgcgttttc
    64321 agacatgtta tttcttatta ctcttaatgc tatttgctat ttcggttagt tttctgagac
    64381 tgtctttttc cttttcaagc atagtaccaa ttacgagggc gtctgctccc gccctgacta
    64441 attcttttgc agtcttggca tcacggattc caccacctac aatcaggaat cctttgaaga
    64501 ccttacgaac agttctgacc atgtcgggcc tgacgttttg ctttgcccca gatccagcct
    64561 ccagatagac aaacctcatg cctaaaaatt gcgcagctaa tgcatatgca gccgcaatgt
    64621 ttggtttgtc aaaaggaata cttcttgccg aaccaacaaa ccaagcagtc gttccctcac
    64681 caattatgat ataggccgtt gggagtggct caagaccaaa tttaagcaca cttggcgcac
    64741 ccaatgcctg tgcttgtgtt atgtagtacg ggttttccga gttcaaaagt gagctaaaca
    64801 agattgcgtc tgcatttgga accacgcctg taacgtttcc aggaaacaaa ataattggaa
    64861 tttttataga tttttttaga gtttttacga cttgggccat ctctaactga tcaatagcag
    64921 aagatccacc aactaagatt gctgaggctc caatccgctc tacactcttt gcaagcctga
    64981 cagattcttt aattttagag gcttcagagt caattagaat gaagattagt gctgattttt
    65041 tcttgcgtgt tacgcgcaag tactcctcta tttttgccat gtctgtggtt tgatcatatt
    65101 gttttaaagt ttaattgcag tgttggtata cagatctgta tagctatggt agagtttgaa
    65161 aaatccaatt ttaataatat tattagtcaa attatcaaaa aatcactgtt tacagagaga
    65221 caacttcaaa ttattctaaa taaaaaaaac ctctccgagt tcaattttaa catctcaaaa
    65281 ggcgcctatt ttaggcaggt tagccagtcc cgagaaaaac ttatgggttt gttttattca
    65341 attattttgc ttcgtggcct tggggttcta ctaccagatg atattgatgt gatatctagg
    65401 cttgcagaac aggtctctgt gataaaggat agtgatgttt tcccagagcg ggaagaacaa
    65461 gtgataggtg ttattgacaa actggttcgt caggcgtgtg gtatgtgatg aatcacatag
    65521 tttttgctac tgtgtttgca atgccgtgat tgtgtctgac attgctgttg atgtaaaatg
    65581 tgaaattatg ttgtcctatc acaataaatc acaactaact actgcataag tgatgttagt
    65641 agagattcct gacccccaga ttatagcagg tgtgataatt gcttttcttg ttggggtttt
    65701 tggaataata atttacaaca aaattaaggt atttgcaggc caaaaaccca cagatccttc
    65761 ctatttttca cgcctagaat attatgaaag gcagctaatt gacatgaaaa tacgtctaga
    65821 tgccttggat ttggaagaaa agataccttt agagtcggtt gaagaaaggt ttgagccagt
    65881 tttggagaaa aaagaggagc gtgttgttaa aaaagaggaa aaacaagttg aaaagccagc
    65941 ccaaaggcca agaatgccca atatggggtt tgatggaata gcagaacatg tcttgggtct
    66001 aatcacgaca aaggaaatga cgtcacgtga catccaaatc acaataggta gaagtcggga
    66061 gcacactgca aggcttatga aaaggctttt tgaggaaggg ttagttgaaa ggaataccaa
    66121 aaacaaaccg ttcacttaca agatcaccgg ccagggaaaa tccaaactcg gtaatttaga
    66181 acaccccatt acggcctaaa ttattggtat tagttgagaa atctttctat gggttggaga
    66241 agttccgatt attctatgtt gtttatccct acttttatta taaaataata ttttaaaaaa
    66301 tcttaaatat cttaaattct ttaattaatt aatgctgtca gaaaatgaag tgttggtcaa
    66361 gatcacggct gtagggacca tttccatacc caaacaattc cgcaagtatc ttggaataca
    66421 aaagggggat tatgtcaagg ttagcttgca agatgatttt ttagttttaa aaagggtaat
    66481 gatttcctaa tctgttcttt ggactatttt tgctatttgg taaacccatt ctcggccgtt
    66541 gcgaagacgg tttattcttc cctttgtgtt aaatcttgcc aaataggtcg aaattatgct
    66601 aagtttaatt ggttcgttgt actcatcctc atatttttcg aggatctcat ttgaggtaaa
    66661 gcctcccatt gggaagtatt tatcaacaat atgccagatt tttgctccaa ctgagtctag
    66721 ttgtggggct gcttcctgtt cttctatatt catcaaatcc atcatctcaa atatcttgag
    66781 aaccttatct ctagtgatat ttccttctat gttaaagttg tatttggcgc cgtcagtatc
    66841 ctccaagtct attcgaatcc gctttctagc cattgtgatc gatcgaatta accagttaac
    66901 aattgaacag atccgatgat atttgttaac aatgagggtt gttaacgttg actgtatggc
    66961 ctacgcctag taggttttcc cttgttaaca actaccttcc tacacaaacc cccgttttgg
    67021 ttaattttat gcctggactg gaaatgttgg ggtcataatt ggctcgttaa caatgttaat
    67081 ggtaagctta acgcctggac tggaaatgtt ggggtcataa ttggcttttt ttgaggtaat
    67141 cttcacatta tattaactgg ttgttaatta gattggaatg ttttgaagaa cacgtcgcca
    67201 aacatatcat agtttgacct ttttgatgcc attgtaaacc caccataata cctcaattgg
    67261 ttcttaccgt attttttaat ttcaattgga tcggttcttt cctttctcca ttgttttaat
    67321 atctccacgg ctacaatttt gttttcttta actgctatgg cgtctggtga tttaccatga
    67381 agtttaataa ctctccaacc atcaaattct aatgttttca ccatctcatc cagggttttt
    67441 tcatggtcag aaccaccaat ttttcccata tatctaacac cacattcaag tataaaacgc
    67501 acacgccttc atttccactc tttcttcttt caaatttttt ttaaaattaa ataattctaa
    67561 taatcatcat taataactta acatcataat catcagtaga aacgtaggtg tctgtgattt
    67621 ctgatcggtg ttaatagaaa cgatctgaaa agtgaaaatt tatcagagga tctcctgaag
    67681 tcctaatcat agatcttaaa tgatctcaat atgagcataa ccatgagcga tccgattgat
    67741 agattacttg atgctgcaga aagcggcaaa tctctaatca aaaatagaga tgtcctacat
    67801 tttacctaca tcccagacgt aatctatcat agagatgaag aacaagaaaa agtaacccaa
    67861 tcactcttac caatcttaaa acaatcaaga ccgtccaacc tacttgttta cggcaagccg
    67921 ggaactggta aaacactggt tatcagaaag gttctatcca aaattcaaac aagggttgaa
    67981 aaaagcagct tcccaataaa aatggcgtat gtgaattcaa agaacgaaac aacgctttac
    68041 ggattattag tgagctttgg aagacaacta acccaaaatc aatccgaaaa agaccgattt
    68101 cctacaactg gccttgcaat cagtgagatc ttcaaacgaa tcctcaaaac aatcaatgat
    68161 aaccacttta acacaatctt tgttatagat gaaatcgatt ttctagctca tttggtatca
    68221 aaaactggta acgatatact ttaccaacta accagagcaa acgagcgtct aaaacaaggc
    68281 tcgttgacat tggtgggaat atcaaacgat cttactttca aagaaagact tgatccgcga
    68341 gtaataagca gtttgggcga agaggaaaca atattcacaa actacagtgt tgaccaaatc
    68401 aaaaaaatcc tagaagaaag aatcaaggaa gcctttgccg aaaacaccat agagaattcc
    68461 gcattaaact tatgcgcagc gatggctggt agagaacacg gtgatgcaag acgagcaatc
    68521 gatctattaa gggttgcagg cgaaattgct gaacgggagc agtctgataa ggtcaaagag
    68581 gagcacattc gaatagcctc acaaaaaatg gaagaagata aagaagttac cgcgctccgg
    68641 tcgtatccac ttcatgaaaa actcctaatc ctcgctgtga tgaaggcctc agggctttct
    68701 actggagaga tctatgctgc atacaaaacc ctctgcaaaa ccacacggca aaaagagcta
    68761 acacaaagac gtattactca aatgctaagc gaaatcgaaa tgtcaggaat aatctcgggc
    68821 aagatagtac atcagggaat tcatggaagg acgaagaaat actcgcttac aatacccact
    68881 gaaacagtaa aggacacctt tagaaacgaa gcaacgcttg aagatgttat ctagtttgag
    68941 gcaaagtctt ttgtgtagac tttgaaggtc ttcaagttaa ctaagatggc taggccagaa
    69001 gtcggcatga ttccaatgcc tacctgaaat ggagtctgcg actgccaagt accagaattt
    69061 acaattagca cgcccttgta tagatcaaaa ccaaccacat gcacatgacc tgcatggaaa
    69121 atatcaggaa cctcatcaat taccataaaa tcttccaatt ctggagcaag tggagtctga
    69181 gccccataaa ttgggcttag gtgtctggcc ttgagcaagc attgcatcac cttggaaggc
    69241 ttgtcgtaac taaggccggg tgtggttttt acaatatcat cgacactttg tccatgaaag
    69301 atcagcactt taacaccatt aagtgaaatc atcgcgggat taccaaccat gaaaaagttt
    69361 tttcgtcccc aaagattcgg gttgtatttt gccggtattg ctggctgggg gagcgctctt
    69421 cttccaggat catggtttcc aggagatatg aagactttga tgtgttttgg gatcttgtcc
    69481 aaaacctcaa agaccaacct taactgctcg cttgtttcaa gggcaactaa ctccttgtct
    69541 tggtttggat agataccaat tccatccacc acatcgcccc caatcaccat aaatctaatt
    69601 tttcgtgcaa caggatcaga gcttgaaagc catgagacca gatctaagaa ttctttttcc
    69661 atgaaaaact tacttccaat atgcaaatcg gaaacaaaca acgcgtatgt ttcagttttt
    69721 gagcgatttg taatgtgatc tgggatgtca ggcaaaatta tatcctttac aataaatcca
    69781 ccgtttttac ttgcgactat cttttccata acaaactgat ccatgaggag agaagaggct
    69841 atcttttgga ggtcctcatc aattactata gtttcgatcg agcctgacgg atcgtcaagc
    69901 gttatttttg taacatttct atccgtcctt ctatctgaaa ctaaaccaca aacatagatt
    69961 tcgtcctttg attttatgga aatggccgaa gagatgggtt ttagcatctt tgcctcgggc
    70021 cgattcgaca ttatcttttt tagcttagcg tacctacttg agaaaagagc attaaatccc
    70081 tccaccccct cagcagaggt aatttttgtg gtggcatcaa acaagatttc atgtgagtca
    70141 tctaattcct catcctcttt aattcctaag aaggattcga gatctttttg tgaaatcaag
    70201 taggcgcgct gtttttcttt ttctcgcaca acttgcctga tgatcccctc taactctttt
    70261 tcatcaacct gctcaagaat cttgagcgca tctggatgta cctgaaaccc acgatttaaa
    70321 gcaaaatcta atgcagcatt gagatcttta atcataatga aacttcatcc aaacaagttt
    70381 taattaaatc tgcgcaaagc aacagactca aataagctca tcaaaaaaac ccgtcgtgat
    70441 taaaaaacga atagtttttt tctttatctt tttagccata ttttcagcgg ctttttcagc
    70501 cggtgctgaa atgactgttc cagaagaaga ggccaaaata tttcttgatg aatttaacaa
    70561 attacttgac gcactacaag gtgaaaattt tggcatcgaa atcttcctac acaacatcca
    70621 gatcgcatta cccatgttta ttcctggatt tggtgtgggg tggggaattt tttctgcatt
    70681 ttcaacaggt tttgcgtttg cagcacttgc aacgaccaca ccacttttga atgaaattcc
    70741 gccacttgca atgatctttg caactccttt tgggctcatg gagcttgccg catattcgat
    70801 tgggatgtca agaagctctc tattgataat cagtctagtt aaaaaaaccc caatctcgca
    70861 acaatggaaa cagaccacaa tcgagatagg gatagtgatt gggttgttgc ttgcaggagg
    70921 gatcattgag gcgtatatga tagagatgtt ttctgtacca gaaaacccgt tttcagaagt
    70981 aaaataacaa ataaaattta gtggctttgc caaaaagaat tcagtagtaa attataaaca
    71041 aaatcaaaac aagactatac catgagatat gttgcgtttg cagcccttgc gttagtaggc
    71101 ataaccctta tcatatcaca agatgcaagc gcgcagcttg cgggaaacaa ggcgttctca
    71161 ctagttggaa atggatttgc agtatctgaa aatgatattt ctgatacaag cgttgatttg
    71221 ttattcacca caaaccaaat gaacaacacg attgacttta gtcttcaaac tggagtgatt
    71281 gtggttggtg ataatgatct aaacatttct gatcttagtg gactgctctt acataaaggc
    71341 aaaatttttc gaattatttc aaatgcagct gattcagaag gaaatcagtt tacagttagg
    71401 atacttggta gaataattga cactgcccaa acagactcga tctacagctt aactggaacc
    71461 ctgacagatt cttcaaaaaa agtaacaaaa cttgttttta caacaaaggt atcggaattt
    71521 acaataaaaa caactgagaa aacatcaaaa tctgccatca ccgttaaaat tctaaaaggc
    71581 tcttcaaacc caggcgaacg aacttacatt gatcaaacag cgggatatac attcaagttc
    71641 ttctcagagg acagaataac aatccctgtt ggggggacaa tcacctttgt aaatgaagac
    71701 accgtatcac acatcctaaa aagcggtact gcaaactatg gctcgcgtca caagacattt
    71761 gatccagatg gtaaaatctc gagtggggac attcaaccag gccagtcttg gagtgtgacg
    71821 tttgacaagc caggatttta cagactattt aatgaaaact atagatggat ggatactaca
    71881 atttttgtga cttcggagac cggttcacag agtctgggtt ctaatataaa accacataac
    71941 tagtctttga aattgatgta cgactctaat tttgcctgat taattaccat cacagaaaga
    72001 tcagaaaact ctttttcttt taggtctttt actgtaccaa caaattctgt ttctttgtcg
    72061 gtggtcaaaa attcaaacac atgcaccctt aagtggctcg tgtcaaaacc attcttttcg
    72121 agatagtaag caatttcaga ctgcataaag tgtttttggg ggttttttgg ccatggccgt
    72181 ggaaccaaaa tcacactaaa cccatcaatg agcgcctttt gtaactctcg ttttttctcc
    72241 tcaataggtg tagttatgtg cattgtaatg attttggatt tgtccaacgg cacttttgcc
    72301 tttgaggcag ctacctgaat tgagctaatt ccagggatta gttgtacatc gccaaaaatt
    72361 tcaattaacc tatctacaac ttctgattct gaaaaattaa catcgcccgt gaatggaact
    72421 actagttttt tgttaccgat tgtctttgca accttttggt aagtttcttc ctgatccttc
    72481 attgttattt cgtagacatc tttatctttg atcagagact cgatggttct taacgtatac
    72541 ttgtaaccaa taataatatc tgagttacgt atcgtctctt tgacaatgtc agttacataa
    72601 gcacttgagc ctggaccgac gccaacagcg taaacagtta tcaatttacc taccttgatt
    72661 atttttgata tattccacaa cggggatcca gtctactttc aaaaatttaa ctggatctct
    72721 gatcggatat gcaacccaat ctttagcaat tgataagtga attttttttt ccttgtcgcc
    72781 atgtctaatt tttaacttta aaactcctcc ccagaccttt tcttcagctt cagcatcaat
    72841 aatatcttct agtggatatt cttcattctt cttattttct aaatccaatt taagattctc
    72901 ttcgccataa ccatcatgag tcaacattat gtcgtttgat ttcataaggg taataatttg
    72961 tctttggacc gcaggtttcc accactgagt ctttgcctca gttttagtta tgaagattac
    73021 atgcttgttt gttaaaaaaa acatacactc tctacctttt gaaaataatt ttttttcttc
    73081 tttccacacc gaaagtaggt gagccttgat tttttcatta ggttccatat cctaaatctt
    73141 tgaaaaagac ttgttaaaaa ctaactgggt ttttagttag acttagttac ttcctatatc
    73201 aagagaatga agctaaacag catgagttac ctccagaaca cactcagtcg tcaggaatgg
    73261 ctggatagaa cctatagaaa gagtaaatcc caaagggcat tagaacttgc ccaagctgct
    73321 ttaaaatcat ttgatgcctt ttgttcggaa aaatatggga aggatcctga tactatcata
    73381 tcggaattaa gaggcaatcc gggtgataga acttacattt tcctaaacaa ttttgtcgca
    73441 tttatggata ataagagccc gaaaacagtc caaacctact ttggtttagt aaaatcattt
    73501 ctccgaactc agggagtcaa aacagatcga gatgatgtta gagaatttgt caagttacca
    73561 actctactta aaatcagacg taagccatta actaaagaga taatccggaa actacttgat
    73621 aattcgaaag aacgaagaaa agccttgtat ctaacccttc tctccagcgg catgagattg
    73681 ggcgaagctt tatcactcaa gaaggagaac ttcgatttta ctaaggatcc tgttatggta
    73741 tttgtacccg cagcctgtac aaagactaga gaatctaggg agacattcat tt
//
LOCUS       DASWKH010000014         9158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3349506, whole genome shotgun sequence.
ACCESSION   DASWKH010000014 DASWKH010000000
VERSION     DASWKH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3349506"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGA92_01490"
     CDS             <1..502
                     /locus_tag="VGA92_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HEX9845119.1"
                     /translation="YRAVTHNKGILNGIIAVANATGQDTRAIEAAAHAYASRTGRYHS
                     LTEWSKDNDGNLVGKIELPMSVGVVGGIINVHSMAKICTKILGVQSAAELACVIAATG
                     LAQNLGAIRALASEGIQKGHMRLHARNLAAAAGAEPAQIDSIVEKMIKEENISINRAK
                     ELVDKK"
     gene            611..859
                     /locus_tag="VGA92_01495"
     CDS             611..859
                     /locus_tag="VGA92_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845120.1"
                     /translation="MKRDLLTVRDVDDEVLDIFKAKAAEHKMKMGKALTEAMQVWVKT
                     KKHPKKDSKMFVKARPFDWGKNTEKTSTQIDDILYDNA"
     gene            846..1241
                     /locus_tag="VGA92_01500"
     CDS             846..1241
                     /locus_tag="VGA92_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9845121.1"
                     /translation="MIMLDTSFVVAYFNTRDQNHDSAIKTAKILARQNHELYVTDYLF
                     GEIVTVSLVRLKNLEKATHIGKIILEYCNLIHVDKEIFDHAWKIFSEQKLRLSFVDCT
                     TIVAMKSQKMQQIATFDNDFNNVEGIEIC"
     gene            1386..1523
                     /locus_tag="VGA92_01505"
     CDS             1386..1523
                     /locus_tag="VGA92_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845122.1"
                     /translation="MTDEVLKLSGKAAKSFLEYDKRELTSDEKESLKKARKYYKDHCD
                     F"
     gene            1725..1943
                     /locus_tag="VGA92_01510"
     CDS             1725..1943
                     /locus_tag="VGA92_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845123.1"
                     /translation="MDSKKEKIYTVLIEQDEDGIYVAKVPDIIGCHTQAKSVSEVMER
                     IKEAIQACSKNESVEPLKFVGIQQIQIE"
     gene            2233..3210
                     /gene="hisG"
                     /locus_tag="VGA92_01515"
     CDS             2233..3210
                     /gene="hisG"
                     /locus_tag="VGA92_01515"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX9845124.1"
                     /translation="MTKVKFAIPKGSLEEATFTILERSWTKVKRKSRTYLVTLDDPQI
                     TVKMLRPQEIPTFVSDGLYDVGITGKDWIAETKSDVQPLLDLEYGKIKLVVAIPDSYR
                     FKSLDEMIASYAKQKKILRISSEYLTTASKYIKQQKSYKKYFGNKDPQIVTPWLRIGT
                     NKNVQIHLSFGATEAKPPDAVDAIMDVTETGTTLEQNQLKILDTVLESTAHLIANKNA
                     LRDKAKREKIYDILTVMRGAVQGRKYLHIYLNVQEKNMSKLLTSLPSLKRPTVSPLSA
                     KGWYGVNTIVPKAEFHKMIPKLRKIAQGLVVHEPRQILELEEIKRDEEN"
     gene            3210..4469
                     /gene="hisD"
                     /locus_tag="VGA92_01520"
     CDS             3210..4469
                     /gene="hisD"
                     /locus_tag="VGA92_01520"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9845125.1"
                     /translation="MKVIFVNNVQAFAKPNYASYDTKRVQSIVNQVRKQGDAALRRFE
                     KKFNGVNVKTLQVSKKEIQGAYKLVTKEQLDAIRLVKTRLEKTESAIKKQLKNITIKS
                     EGTTIEKSFIPLESVGCYVPGGLARYPSSAVMSITPAKIAGVKNIVVVTPPNKQGNID
                     PVVLVAADICGADKIFKVGGAQAIAALAFGTKSIPAVDKIVGPGGSFVTAAKSLIANV
                     TSIDMLAGPTELTIIADDSANPKLVALDLISQSEHSTDTSCYLITTSKTLAGRVIDII
                     KKRAPSLKRKEIVESSLKKNGAVFVCKKVSDAISVANNLAPEHLQIITKNPNQIAKQI
                     KNAGLVLIGQDTPSAASDYLLGTNHILPTNRFGKSRGSLSVLDFVKIKTSVESSKPAL
                     KRIGRNLQVLTKSENLPNHYEAVRSRL"
     gene            4466..5536
                     /gene="hisC"
                     /locus_tag="VGA92_01525"
     CDS             4466..5536
                     /gene="hisC"
                     /locus_tag="VGA92_01525"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX9845126.1"
                     /translation="MTKDWFYKRLQELSKLQGYQKPEYHQGVLKLDSNENFVINKQLQ
                     QELVDSAQKISDIREYPLGKSEKLVEALSRYLQIPSSMIGLGNGSDQILDLILSNFAT
                     KKTKILTSDPTFGFFEERCKLYAIPTIKVPFSKDMTLSLNDFVSKSKKADILYIDSPN
                     NPTGFQFKKEDLTELIKKSDGIAIIDEAYGEFSDYSVVNLVKKFDNLIVVRTFSKTFG
                     LAGLRLGYVVASKKFTDVLTRVLQYPYPLNTLAIEAGILALQKAKQVSETIKIIKQER
                     LRIIKKLRESGAFDVFDSKANFVLFDARGADKRIYTALLEQGISIRKLGKIGKHEGCL
                     RVTVGTKDMNSKFLLAIRDLLQ"
     gene            5533..6510
                     /locus_tag="VGA92_01530"
     CDS             5533..6510
                     /locus_tag="VGA92_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9845127.1"
                     /translation="MMYNNKGQDIFIDETISNIIDQIDGIIFDCDGVLVDVSKSYDLA
                     IRQTTAFVLKKFANLESIPITTEIINGFKDTGGFNDEVDVTYASILSLSAAKKLGANP
                     EKFIFDVIKNADETGIESVEKFLDSQNVDLSEIRKKLDYPSFRLTNPLIQIFDQIFYG
                     QALYEKIFHKKSQFVEPGLIENDLVLITAELLSSLKKKFGNKIAIVTGRGKESISYSL
                     KELLGQFNVDCCVFLEDEPRGLAKPNPKSLIMSFEKLNTSHCLYVGDSMEDLMMANEA
                     NKMGRKITFCGIYGTNKNPESKRQFFEKNQAPLILESINYLPKALNLAN"
     gene            6517..7107
                     /locus_tag="VGA92_01535"
     CDS             6517..7107
                     /locus_tag="VGA92_01535"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX9845128.1"
                     /translation="MFVARTASIKRETKETSVTVQVNLDGTGKTSVNTGIEFFDHLIG
                     SFGKHAMLDLTVKAKSNDKILHHLIEDTGITIGAALDKALGERKGVVRFAYASVPMDE
                     SLAEASIDLVRRQYNKINLSIKRSQIEGMSKEDIEHFVFSLTQNINSCIHLNVKYGEN
                     DHHKIEAAIKSLAVAFRIAASLDKKQKGIPSTKGSM"
     gene            7108..7713
                     /gene="hisH"
                     /locus_tag="VGA92_01540"
     CDS             7108..7713
                     /gene="hisH"
                     /locus_tag="VGA92_01540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9845129.1"
                     /translation="MKVAIFDYGAGNIFSLKIALEKQGATVDVITNFDSANNYSGLLL
                     PGVGNFDPAIRSIRDYSSTSFADYVKDKTPVLGICLGMEMFFEKSEEGKEKGLAVMDG
                     EVILLPNKFKIPHMGWNSIRIKKSNPLLDDIPDSSWMYFVHSYRVKPKNADVVVADSD
                     YGIEVPAVINKGNLFGTQFHPEKSGKIGSIMIRNFLRECKK"
     gene            7710..8417
                     /gene="hisA"
                     /locus_tag="VGA92_01545"
     CDS             7710..8417
                     /gene="hisA"
                     /locus_tag="VGA92_01545"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX9845130.1"
                     /translation="MKIIPAIDLMEGQVVRLVKGKPEDKTIYSNNPQEIAKKWEKAGA
                     DMLHMVDLDATLQRGSNLGMIQKIAKEVSIPIQVAGGLRDESTVSTALDFAERVVIGT
                     IAFNDAKLVSDLAEKFGKKRLVIAVDHNDEIIVINGWQRSTGINLGDAVADFTKKNFT
                     EFLLTNVSRDGTLQGPDIENLSKICKVENTNVIASGGISNSSDIIKVKQCNAYGVILG
                     KALYDGKITIEEAKKLA"
     gene            8414..9124
                     /locus_tag="VGA92_01550"
     CDS             8414..9124
                     /locus_tag="VGA92_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEX9845131.1"
                     /translation="MSLTKRVIPCLDVANGRVVKGKPEDKTVYSNNPQEVAKKWEKKG
                     ADMIHLVDLDATLQRGSNLGTIQKIAKEVSIPIQVAGGLRDESAVSTALDFAQRAVIG
                     TIAFNDIKLVSDLAEKFGKKRLVIAVDHNDGIIVINGWQRSTGINLGDAVADFTKKNF
                     TEFLLTNVSRDGTLQGPDIENLYKICKVENTNVIASGGISNSFDIIKVKQCNAYGVIL
                     GKALYDGKITIEEAKKLA"
BASE COUNT         3264 a         1713 c         1881 g         2300 t
ORIGIN      
        1 gtatcgagca gtgacacaca acaaggggat actaaacggc ataatcgcag tagcaaatgc
       61 aacaggacaa gacacgcgcg caatagaggc tgcggcacat gcatatgctt caagaactgg
      121 aagatatcac tccctcacag agtggagcaa agacaatgac ggaaatctgg ttggaaaaat
      181 tgagctgcca atgtcagttg gtgtggttgg cggaataatc aatgtccatt caatggctaa
      241 aatttgcaca aaaatacttg gtgtccaatc tgctgctgag ctagcatgcg ttattgctgc
      301 tactggcctg gctcaaaatc ttggcgcaat tcgcgcttta gcctcagaag gaatccaaaa
      361 aggacacatg aggctacatg cacgaaactt ggctgcagcc gcaggagcag agccagctca
      421 gattgattcc atagtagaaa aaatgatcaa ggaagaaaac atctcaatta atcgggcaaa
      481 agaactcgta gacaaaaaat aagatccaga aatttcggtg tattgttttt tgatatgact
      541 aatcgaattt gtatggtttt attaaaatga ttcaaatatg tttttatact atgtaataca
      601 tgtattacat atgaaaagag acctgttgac agtccgtgat gttgatgatg aagtgcttga
      661 tatattcaaa gcaaaagctg cggaacataa aatgaagatg ggcaaggcac tcactgaggc
      721 aatgcaagtt tgggtaaaaa ccaaaaaaca tcccaaaaaa gatagcaaga tgtttgtcaa
      781 agctcgtcca tttgattggg gaaaaaatac agaaaagaca agcactcaga ttgatgatat
      841 tctatatgat aatgcttgac actagctttg tagtagcgta tttcaataca agagatcaaa
      901 accacgactc tgcaataaag actgctaaaa tactagctcg ccagaaccat gagctttacg
      961 taactgatta cctatttgga gagattgtca cagtaagtct tgtcagacta aagaacttgg
     1021 aaaaggcaac acatatagga aaaataatct tagaatattg caatttaata catgttgata
     1081 aagagatctt tgatcatgca tggaaaatat tctcagaaca aaaactacga ctaagctttg
     1141 tagattgcac tacaattgta gcaatgaaaa gccaaaaaat gcaacaaatt gccacatttg
     1201 ataatgattt taacaatgtt gagggaatag agatctgttg aagtaaacct ctaccgcctg
     1261 tccggcatat taggttacga gttaaattca aagaggaaaa agcaataatc tttggttgtc
     1321 tgtttaggag tccttaacaa gctttttaaa gtttgtaaaa gaacttgtaa ttaagcgtga
     1381 ccaagatgac cgatgaagtt ttaaaattaa gtggtaaagc ggcaaaatct tttctggaat
     1441 atgataagcg tgagctgact tctgatgaaa aagaatctct caaaaaagca cgcaagtact
     1501 acaaagatca ttgtgatttt tagttgtgtt taagaacaat ctaatcttct attaatcgta
     1561 ttgaatttac gttacaagtt gctctctgat gaagagtctt ttccacaatt gaattttaca
     1621 aatggtgatg tatagacgat tagtttgatc agaaaaagga atttgacaga gtcaaaagga
     1681 ctatcaacag cacaacttaa aaaatatccc attgcatgta ttctgtggat tccaagaagg
     1741 aaaaaattta caccgtgcta atagaacaag atgaagacgg aatctatgta gcaaaggttc
     1801 cggatattat tggttgtcac actcaagcca aatctgtttc tgaggtcatg gaacgaatca
     1861 aagaagcaat ccaagcttgt agcaagaatg aatctgttga acctctcaag tttgtcggga
     1921 tccagcagat tcagatagaa tgaaacctgg tgttatttct ggcaagtact accacttggc
     1981 aaattctgtg aagaatttcg atgccatgga tttttctccc ttatcacttt ctatttcctt
     2041 ccacgcatca ttgattgatt tgagcgtctc aatatcatct tgcttcagtt gcttgttttg
     2101 cttttttaaa ataattcact cctttctatt attacaagta cattccaatc ttttatgtca
     2161 atcttttctc atggcggcgc tatggttttc tcgtcgcttt gtttatagaa tgctagcagc
     2221 ccaacattgc atatgaccaa ggtcaaattc gcaattccaa agggcagtct ggaggaggca
     2281 acatttacca tcctggagag atcctggact aaggtgaaaa gaaaaagccg cacgtaccta
     2341 gttacactag atgaccctca aatcacagtc aaaatgctca ggccccagga gatcccgacg
     2401 tttgtctcag acggcctgta tgatgttgga attactggaa aagactggat tgcagaaacc
     2461 aagtcggatg ttcaaccgct tttagattta gaatatggca aaataaaact agtagtcgca
     2521 attcctgact cttaccgatt caaatcactt gatgaaatga tagcatcata tgcaaaacaa
     2581 aaaaagatcc ttcgaatttc ctcagaatat cttaccaccg catccaagta catcaagcag
     2641 caaaaatcat acaaaaaata ctttggaaac aaagacccgc aaatcgttac accatggctt
     2701 cgcatcggca caaacaaaaa tgtgcaaata cacctctcgt ttggcgcaac tgaggcaaag
     2761 cctcctgatg cagtagatgc aataatggat gtaacagaaa caggaactac gcttgagcaa
     2821 aaccagctga aaatacttga tactgttttg gaatcaactg cacacctaat tgcaaacaaa
     2881 aacgcactac gtgacaaagc aaaaagagag aaaatctacg acatactaac tgtgatgcgc
     2941 ggcgcagtgc agggaagaaa atacctgcac atctacctta acgtacagga aaaaaacatg
     3001 tcaaagctac tgaccagtct gccctcactt aagaggccta cagtaagccc acttagcgcc
     3061 aagggctggt acggagtaaa cacgattgtt ccaaaagcag agtttcacaa aatgatccca
     3121 aagctgcgca aaatagctca agggctggtt gttcacgagc caagacaaat actggagctt
     3181 gaggagataa agcgtgatga agaaaattga tgaaagtcat attcgtaaac aatgtacagg
     3241 catttgcaaa gcctaactat gcaagctatg acacaaaacg agtccaatca atcgtaaacc
     3301 aagtaagaaa gcagggtgat gctgcacttc gacgctttga gaaaaaattc aacggcgtta
     3361 acgtaaagac actccaagtc tcaaaaaaag agattcaggg cgcatacaaa ctagtgacaa
     3421 aagagcagct tgacgcaata aggctagtta agactagact ggaaaaaact gaatcggcaa
     3481 taaaaaaaca gctaaaaaac atcacaataa agtcagaagg cacaacaatt gaaaaatcct
     3541 tcattccact ggaaagtgta ggatgctacg taccaggggg tcttgcccgt tatccaagct
     3601 cagctgtaat gtctataact ccagcaaaaa ttgctggcgt aaaaaatatt gtagtagtca
     3661 caccgccaaa caaacaagga aacatagatc cagttgtctt agtggcagct gacatttgtg
     3721 gcgcagacaa gattttcaaa gttgggggcg cacaagcaat tgcagccttg gcatttggta
     3781 caaaatcaat tccagcggtg gacaaaatag tgggtcctgg agggtctttt gttactgcag
     3841 caaaatcact tattgcgaac gttacctcaa ttgacatgct ggcaggccca accgagctta
     3901 caataatagc tgatgattct gcaaatccaa aacttgtcgc acttgatctt atttcacagt
     3961 cagagcacag cactgataca tcatgctatt tgataactac atcaaaaaca ctcgcaggca
     4021 gggtaattga tatcatcaaa aaaagagctc caagcttgaa aagaaaggag attgtagaat
     4081 caagcctcaa aaaaaatggg gctgtttttg tctgcaaaaa agtctctgat gcaatatcgg
     4141 ttgcaaacaa tcttgcaccc gaacacctac aaataatcac aaaaaaccca aaccagatag
     4201 caaaacaaat caaaaatgca ggattggtcc tgattggcca agatactcca tcggctgcaa
     4261 gcgactatct gcttgggaca aaccacattc tgcctacaaa cagatttgga aaaagcaggg
     4321 gctcgctttc agttcttgac tttgtaaaaa taaaaacaag cgtggaatca tcaaaaccag
     4381 ccttgaaaag aataggcagg aaccttcagg tattgacaaa atcagaaaat ttgccaaatc
     4441 actacgaggc agtacggagc aggctatgac aaaggactgg ttttacaaaa gattgcagga
     4501 attatccaaa ttacagggat accaaaaacc ggaataccac caaggagttc taaaactgga
     4561 ttctaatgaa aactttgtaa tcaataagca actccaacaa gaactagtag atagtgcaca
     4621 gaaaatctca gatatacgcg agtatccact tggcaagtca gaaaaactag ttgaagcact
     4681 ttcaagatat ctacaaattc catcttcaat gattggtctt gggaatggtt ctgatcaaat
     4741 ccttgatctt atcctttcaa actttgcaac aaaaaagacc aaaatactga cttcagatcc
     4801 aacatttgga ttttttgaag aacgctgcaa gctgtacgca atacctacca tcaaagttcc
     4861 attctcaaaa gacatgacat taagtcttaa tgactttgta tcaaagtcaa aaaaagccga
     4921 cattctatac attgattcac caaacaatcc aactgggttt cagttcaaaa aagaagatct
     4981 tacagaatta ataaaaaaat ccgacggaat agcaattatt gatgaagcat acggggagtt
     5041 ttccgactac tctgtcgtaa atcttgtaaa aaaatttgat aatttgattg tggttagaac
     5101 attttcaaag acgtttggac tggcaggact gcgactaggc tatgttgttg caagcaagaa
     5161 gttcaccgat gttcttacac gagtgcttca atacccatat ccactaaaca cactggcaat
     5221 agaggcaggc attttagcgc tacaaaaggc aaaacaagtc tctgagacaa taaagataat
     5281 caaacaggaa cgcctgcgaa tcatcaaaaa attgcgtgaa agcggtgcat ttgacgtctt
     5341 tgactctaag gcaaactttg ttttatttga tgctaggggg gcagacaaac ggatttacac
     5401 cgctctctta gaacaaggaa tctctattcg taaactaggc aagattggaa aacatgaggg
     5461 atgcctacgt gtaaccgttg gcacaaaaga catgaactcc aagtttttgc ttgcaatacg
     5521 tgatctttta caatgatgta caacaacaaa ggccaagaca tcttcataga tgagaccatc
     5581 tctaacatca ttgatcaaat agatggaata atctttgatt gcgacggagt tttagtcgat
     5641 gtatccaagt cgtatgatct ggcaataagg cagacaacgg catttgttct aaaaaaattt
     5701 gcaaatctgg aatcaattcc aatcacaact gagataatca acggcttcaa agatactggg
     5761 ggattcaacg atgaggtgga tgtcacatat gcatcaatac tttctttgag tgctgcaaaa
     5821 aaacttggag ccaatccaga aaaattcatc tttgatgtga ttaaaaatgc agacgaaact
     5881 gggattgaat cggttgaaaa atttctggat tctcaaaatg tagatttgtc agaaattaga
     5941 aaaaaactgg actatcctag ctttcgctta acaaacccac tgatccagat ctttgatcaa
     6001 attttttatg gccaggcatt atatgagaaa atctttcaca aaaaatccca atttgtagaa
     6061 cctggactaa ttgaaaatga tctggttctt atcacagccg aacttttatc ttcgctaaag
     6121 aaaaaatttg gaaacaagat tgccatagta acggggcgtg gcaaagaatc aatcagctac
     6181 tctctaaagg agctgttggg tcaatttaat gtggattgtt gtgtttttct tgaggatgaa
     6241 ccaagggggc ttgcaaagcc aaacccaaaa tccctaatca tgtcttttga gaagctaaat
     6301 acaagtcact gtctctatgt tggtgattca atggaagacc tcatgatggc aaatgaggca
     6361 aacaaaatgg ggagaaaaat caccttttgt ggcatttacg gcacaaacaa aaacccagaa
     6421 tcaaaaagac agttttttga aaaaaatcaa gcgccactca tacttgaatc cataaattat
     6481 cttccaaagg cattaaattt agcaaactag gattcgatat ttgtggcaag aacagctagc
     6541 attaagcgag aaacaaagga aacctcggtt acggtacaag taaatctgga tggtactggc
     6601 aagacttcag tcaatactgg aatagagttc tttgatcact tgataggctc ttttggaaaa
     6661 cacgcaatgc ttgatttgac agtaaaggca aagtcaaatg ataaaatttt acaccaccta
     6721 atagaggaca ctggaattac aattggagct gcactcgaca aggcacttgg ggaaagaaaa
     6781 ggagtggtga gatttgccta tgcctcagta ccaatggatg agtccttggc agaggcatca
     6841 atagacctag ttaggcgtca gtacaacaaa atcaacctct caatcaaacg ctcacaaata
     6901 gaaggaatgt caaaagaaga cattgaacac tttgtctttt ctttgactca gaacatcaac
     6961 agctgcatcc acctaaacgt caagtatggg gaaaatgatc accacaaaat agaggctgcc
     7021 atcaagtcct tggcagttgc atttaggata gcagcaagtt tggataaaaa acaaaaggga
     7081 ataccaagca caaaaggttc aatgtagatg aaagtagcga tatttgacta cggtgcagga
     7141 aacatattca gcctcaaaat agcgctggaa aaacaaggtg ctacagtaga cgtcattaca
     7201 aattttgata gtgcaaacaa ttactcgggc ttgttgctac caggagttgg aaactttgat
     7261 ccggcaattc gaagcatacg ggattactct agtacctcct ttgcagatta tgtgaaagac
     7321 aaaactccag ttctaggaat atgtcttggt atggaaatgt tttttgaaaa aagcgaggaa
     7381 ggaaaagaaa aagggcttgc agtaatggat ggtgaggtga tccttcttcc aaacaaattc
     7441 aaaataccac acatgggctg gaacagcatc agaataaaaa aatcgaatcc acttttagat
     7501 gacataccag attcttcgtg gatgtatttt gttcactcgt acagagtaaa gccaaaaaat
     7561 gctgatgtgg tagtcgcaga ttctgactat ggcattgagg ttccagcggt aatcaacaaa
     7621 ggaaacctgt ttggtaccca atttcatccg gaaaaatcag gcaaaattgg ttccattatg
     7681 atccgaaatt tcctgcgaga gtgcaaaaag tgaagataat tccggcaatt gatctgatgg
     7741 aaggccaggt agtcagacta gtaaagggaa agccagaaga caagacgatc tacagtaaca
     7801 acccacaaga gattgcaaaa aaatgggaaa aggctggggc agatatgcta catatggtag
     7861 accttgacgc aacacttcag cgtggctcaa accttggaat gattcaaaaa atcgcaaagg
     7921 aggtgtcaat accaattcaa gtcgctggtg ggttgcgtga cgagtctacg gtaagtacgg
     7981 ctttggactt tgcggaaagg gtcgtaatag gcacaattgc attcaatgat gccaaattag
     8041 tttcagactt ggcagaaaaa tttggaaaaa aaaggcttgt aatagcagta gaccacaacg
     8101 atgaaataat cgtaataaat ggatggcaac gatctactgg aataaacctt ggtgatgcag
     8161 ttgcagattt taccaaaaaa aactttaccg agtttttact gacaaatgta tcgcgagacg
     8221 gtacactgca aggaccagac attgaaaacc tttccaagat atgtaaggta gaaaatacaa
     8281 acgtgattgc aagcggtgga atctccaatt cttctgatat aataaaagta aaacaatgta
     8341 atgcatatgg ggttattttg ggaaaggcac tctatgatgg aaaaattaca attgaggagg
     8401 caaaaaagct agcttgagtc taacaaaaag agtaattccc tgtcttgatg tagcaaatgg
     8461 cagagtagta aagggaaagc cagaagacaa gacagtctac agtaacaatc cacaagaggt
     8521 tgcaaaaaaa tgggaaaaga aaggagcaga catgatacac ctagtagacc ttgacgcaac
     8581 acttcagcgc ggctcaaacc ttggaacgat tcaaaaaatc gcaaaggagg tgtcaatacc
     8641 gattcaagtc gctggtggat tgcgcgacga gtctgcggta agtacggcct tggactttgc
     8701 acaaagggcc gtaataggca caatcgcatt caatgatatc aaactagtct cagatctggc
     8761 agaaaaattt ggaaaaaaaa ggcttgtaat agcagtggat cacaacgatg gtataattgt
     8821 aataaatgga tggcaacgat ctactggaat aaatcttggt gatgcagttg cagattttac
     8881 caaaaaaaac tttaccgagt ttttgctgac aaatgtatcg cgagacggta cactgcaagg
     8941 accagacatt gaaaaccttt acaagatatg taaggtagaa aatacaaatg tgattgcaag
     9001 cggtggaatc tccaattctt ttgatataat aaaagtaaaa caatgtaacg cgtatggggt
     9061 tattttggga aaggcactct atgatggaaa aattacaatt gaggaggcaa agaaactagc
     9121 atgagtctaa caaagagagt gattccatgt cttgatgt
//
LOCUS       DASWKH010000015         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2248068, whole genome shotgun sequence.
ACCESSION   DASWKH010000015 DASWKH010000000
VERSION     DASWKH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2248068"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1446)
                     /locus_tag="VGA92_01555"
     CDS             complement(100..1446)
                     /locus_tag="VGA92_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845132.1"
                     /translation="MELDSEKIAEIRKKVQKQNTIFKDKRYLDSLFLPSKIIGREKQA
                     EQLIQYINSLKQGLLVPILSVYGRSGTGKSTVVRFVCKNLSDMLCFSFVNLRKAKTVF
                     GCANLILSELGFANLKSAQGMNKAIDLMEQKIQEILKKEDKKFFVLALDEYDVIFSDT
                     RGRPSDFVYKLLTLEENLREKGFWLCIITISNNALADNDLDDRVKSRMGNSEIFFEPY
                     KEDDIFNILRDRAKKAFAKKVDDSVLQHCARLSTDDHGDARRALDLLRVAAELSDGTN
                     VTKAEIEKAVDFIQKDRISTIISNAALHQRVVIAAICACSLLNEYAWNSTSSIYEKYK
                     KYVGKNAKPLSYRRIVDLLVEIKNSGLVESRTLSKGRGGYGTEYKLKLSPEMVGPFVD
                     KKWWDSIVLDKIEYEAELKHRKMMDGLSTSPGYKMLRKQIDRMSEEQWRKKIGLDT"
BASE COUNT          453 a          361 c          268 g          552 t
ORIGIN      
        1 catctgtcat aaaccttgta catgccgtct ttgtcaaatt tgttcagagc cgaaagatca
       61 atcaattata atttcggagt aaagttacaa tatattgatt taggtgtcaa gaccaatttt
      121 tttgcgccac tgttcttctg acattctgtc tatttgtttt ctcagcattt tgtagcctgg
      181 gcttgtagac aggccatcca tcatctttct gtgtttgagc tctgcctcgt attctatttt
      241 gtctaataca atcgagtccc accacttttt gtccacaaac ggccctacca tctcaggcga
      301 aagttttagc ttgtactctg ttccatatcc gccccttcct tttgataagg ttcttgattc
      361 cacaaggccg gagtttttaa tctcaactaa aaggtccact atacgtctat acgataacgg
      421 ttttgcattt tttccaacat attttttata cttctcatag attgaggatg tagagttcca
      481 ggcatattca ttcaaaagag agcaggcaca aattgcagca ataactactc tctggtgcag
      541 tgccgcattt gatataatgg tggaaattct atccttttgg ataaaatcaa ctgccttttc
      601 aatttcggct tttgttacat tggtaccatc tgaaagctca gcagcaaccc gaagcaaatc
      661 aagtgcgcgt cttgcatcgc cgtgatcatc ggtgctaagc cttgcgcaat gctgtaaaac
      721 agaatcatct actttttttg caaacgcctt ttttgccctg tcacgcagaa tgttaaaaat
      781 atcatcttcc ttgtatggct caaaaaatat ttcagagttt cccatcctag actttaccct
      841 gtcgtcaaga tcattgtcag ccaaagcatt gtttgatatt gtgattatac aaagccaaaa
      901 tcccttttct cgaaggttct cctcaagcgt tagcagtttg tacacaaagt cagatggcct
      961 cccacgagta tcagaaaaga ttacatcgta ttcatcaagt gccaaaacaa aaaatttttt
     1021 gtcttctttt ttcaaaattt cctgaatttt ttgctccatc aagtcaattg ccttgttcat
     1081 tccctgagcg cttttcaggt ttgcaaatcc aagctctgat aaaatcaaat tggcacatcc
     1141 aaacacggtt tttgcctttc taaggtttac aaaagaaaag caaagcatgt ctgacaaatt
     1201 cttgcaaaca aaccgtacta cagttgattt tccagtaccg cttcttccat agactgatag
     1261 tatgggaaca agcagtccct gctttaggct gttaatatac tggattagct gctctgcctg
     1321 cttttcacga ccaatgattt ttgatggcaa aaacaaggaa tcaagatatc ttttatcctt
     1381 gaagatagtg ttttgttttt gaaccttttt cctaatttct gctatttttt cagaatctag
     1441 ttccacttgc aacgcatata tcaataaaac aaagcccgtt tctcgtactt aatgaccaaa
     1501 tggcaataaa atttgacacc gcttgagttt taagcaacaa attaatcctc ataaccctgg
     1561 ttttgggcaa aaaattcaag cttggatttt tgccatgacc ttgccaaaaa ttcgctctgc
     1621 cgattttatc gagt
//
LOCUS       DASWKH010000016         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_771441, whole genome shotgun sequence.
ACCESSION   DASWKH010000016 DASWKH010000000
VERSION     DASWKH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771441"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VGA92_01560"
     CDS             <1..984
                     /locus_tag="VGA92_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9845133.1"
                     /translation="GSAVAVSAFESVTGLGSDTGGSVRNPASFCSIVGIKPTYGLVSR
                     YGLISYANSIEQIGPMARTVKDAAFLLNIISGIDPNDNTTVNSHGEDYLKDIESGIDG
                     KKIGVISEMVGKGIDKEVSSATKKALAKFEDLGAKCEEISIDMVDYSVASYYTITSTE
                     AGSNLARYDNLRYGFDFDLEGYEFNSYISKARKNFGPEVTRRLIIGGFVPSAGHAGKY
                     FLKALKVKSKLTKEINSAFKNVDFLVAPTVPVLPFKFGEKIDDPIAMFLIDFNTSTAN
                     LSGKPAISVPFEISNGLPIGIQIIADSMQEKSLFQAAYALESTTNLPKVPL"
     gene            981..>1573
                     /locus_tag="VGA92_01565"
     CDS             981..>1573
                     /locus_tag="VGA92_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX9845134.1"
                     /translation="MTKIGLEIHCQLTKLESKLFCSCKADYRGFEPNTNICPVCVGLP
                     GSLPRLNKKAIEKATVIAMALNCQTPPKIAFFRKNYFYPDLPKNFQITQLNIYGPTSI
                     GQEGKISVEGKEIQIRRIQLEEDPGRLIYEGSSERTQITLVDYNRAGTPLVEIVTEPD
                     FENPKQVRLFLNILSDLLENLGVSDPSLEGAMRADANV"
BASE COUNT          541 a          275 c          315 g          442 t
ORIGIN      
        1 gggagcgcag ttgcagtcag tgcatttgaa tctgtcacag gacttggctc cgatactggt
       61 ggttctgtaa gaaacccagc tagtttttgt tcaatagttg gtatcaaacc aacatatggt
      121 cttgttagca gatatggtct gatttcatat gcaaacagca ttgaacagat tggtccgatg
      181 gcaagaactg taaaagacgc tgcatttctt ctcaatataa tttctggaat agatcctaat
      241 gataatacta cagttaacag ccatggtgaa gactatctaa aagacatcga atctggaatt
      301 gatggaaaaa aaatcggagt aatttctgag atggttggaa aaggaattga caaagaagta
      361 tcttctgcaa caaaaaaagc acttgcaaaa tttgaagatc ttggagcaaa atgcgaagaa
      421 atttctattg atatggtaga ttattctgtt gcatcatact atacaattac atcaacagaa
      481 gccggaagca accttgcaag atatgacaat cttcgttatg gttttgattt tgatttagaa
      541 ggatacgagt ttaactcata catttcaaaa gcaaggaaaa attttggacc tgaagtaaca
      601 aggagattaa tcattggcgg gtttgtacca tctgcaggtc atgctggaaa atattttcta
      661 aaggcattaa aagtaaaaag caaattaaca aaagaaatca attccgcatt caaaaacgta
      721 gattttcttg tagcaccaac agttccagtt ctcccattca agtttggaga aaaaatcgat
      781 gatcctatag caatgtttct aattgatttt aacacatcaa ctgcaaatct tagtggcaaa
      841 cctgcaattt ctgttccatt tgaaatctca aatggtctgc caattggaat tcaaattatt
      901 gcagactcga tgcaagaaaa atcgcttttt caggcagcat atgctcttga gagtacaaca
      961 aatcttccaa aggtgcctct atgacaaaga ttggtcttga aattcactgt cagttgacaa
     1021 agcttgagag caagctgttt tgttcttgca aggcagatta tcgtggattt gagccaaaca
     1081 caaacatctg tcctgtatgt gttggcctcc caggctcgct tccaaggcta aacaaaaagg
     1141 ccattgaaaa ggccacagtt atagcaatgg cattaaactg tcaaacaccg ccaaaaattg
     1201 cattttttag aaaaaactac ttttatccag atttgccaaa aaactttcag attacacagc
     1261 taaacatcta tggtccaaca agcattggcc aagaaggaaa aatcagtgta gaggggaaag
     1321 aaattcaaat cagaagaatt cagcttgaag aagatcctgg caggttaatt tatgaaggaa
     1381 gttcagagcg aacccaaatt acgcttgttg actataaccg tgcaggaacg ccacttgttg
     1441 aaattgtaac agaacctgat tttgagaatc caaaacaggt gagattattt ttgaatatat
     1501 tatcggattt actcgaaaac ttgggagtgt cagatccgtc acttgaaggt gcgatgaggg
     1561 cagatgcaaa tgt
//
LOCUS       DASWKH010000017        13379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3353177, whole genome shotgun sequence.
ACCESSION   DASWKH010000017 DASWKH010000000
VERSION     DASWKH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353177"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VGA92_01570"
     CDS             complement(<1..1379)
                     /locus_tag="VGA92_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845135.1"
                     /translation="MNAAIKLVIIISSVLLASGIATEDAFAKTAKGTANQPFNEEQVC
                     GDRLCGPDEKLSIKEKIGYYLLSFLEFDESTVIPQSRFQISGVTQQGTGILTTGNVMI
                     KDFSTSTIIDRGKVNSLAVPTTQVGSKQTSTMKIDSANLAGNIRISNIDRDRFVALVD
                     RTNVLNVNVFPDTTTLKIIKDGTPMKVKQANLVCPSATMWNGYKCVSVKLLQLSCDSL
                     PKEKKLLCYQLVEKRATETFTLSKTYKLDSSKQQALSTRVYSDTTNLVKSISNYNSNV
                     KVLCKANDFGTIRCADMLSLLSFYDQTIGKSIMEDIDAIYESTELNRFDEMLIDCGSG
                     TWATHDIEDTKTQPNNWESGAYEDMVTEEDGEEISDKCSSANSGGKSSETSTSSGFSG
                     MTGIDTMGGMCGSEFNFYTKDDMANKFVEMVGDYRQQCGQWIGSGVAAGDGGSDSATP
                     TPPPPPPPPP"
     gene            complement(1746..2345)
                     /locus_tag="VGA92_01575"
     CDS             complement(1746..2345)
                     /locus_tag="VGA92_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX9845136.1"
                     /translation="MADSPNLLLFRKWDLDGIEIKDPGLKSVISLKKAIMPLTFGRSS
                     LKRFNKADVNIVERLANKLMHFGKKYAKNTGRMGGKKTRSINTVKAAFEIINLKTGKN
                     PVEVLVRAIEHSSPNEDTTRIVYGGTAYHVSVDVSPLRRVDLALRFIADGVKESSFSN
                     PKAMEEYLAEHLISASTNDAGAPSVKKKNELERIAQASR"
     gene            complement(2350..2787)
                     /locus_tag="VGA92_01580"
     CDS             complement(2350..2787)
                     /locus_tag="VGA92_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9845137.1"
                     /translation="MAKSPLGLFAGRVLKTKRKKQRWAIGVYKRRMLGLDKKASPLGG
                     SPQARGIVLEKVGVEAKQPNSAVRKCVRVQLIKNGKTVTAFLPRDGAMNFIDEHDEVH
                     LEGMGASMGGAMGDIPGVRFKVFKVNGTSLRELVRGRKEKPRR"
     gene            complement(2792..3250)
                     /locus_tag="VGA92_01585"
     CDS             complement(2792..3250)
                     /locus_tag="VGA92_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HEX9845138.1"
                     /translation="MPQTIKLTTDQMRLISLFQNVTGASARDCVEDEKQNRVIFVVNE
                     GKMGLAIGKGGSHIKNLQNIIKKNVELVEYSDDPIEFLKNMLNSKLVTEVKLNKRLDG
                     TTQAIVLVDPKKKGIVVGREGRNAEKARLLARRYFEITSVLINSPDKFTE"
     gene            complement(3319..3627)
                     /locus_tag="VGA92_01590"
     CDS             complement(3319..3627)
                     /locus_tag="VGA92_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEX9845139.1"
                     /translation="MGKLLEKALKDALQESKCVLGVKQVLQSIKNAKLVVVSKSASKE
                     SIDKIESSKDQKVTTLQFDGSSVALGKVCGLQFRVSAASLTSIPDSNIKAILKESEVQ
                     "
     gene            complement(3717..4895)
                     /locus_tag="VGA92_01595"
     CDS             complement(3717..4895)
                     /locus_tag="VGA92_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845140.1"
                     /translation="MLALIGVVFLVIGAYAEQIPDYNKPYAPIYTDKEVYSWTDKIRI
                     TIAAPSWNENKYGIDSIGDDASHPVKISTSSHSLEPYRLTETAPNSGIFTGEVVLTGF
                     LHDADGDGQSDTQPRTMGVGPTSGLLETKRDDGITISFEFADGVVLTKSVKVSWNFAE
                     IEFSNPNYLVTDQVVIQVRDADMNLNPESLDQVNVDVSSDSDSAGISVAATETGEDSG
                     IFEALVVLTQTNDSSGNRLLAIPGNTITARYEDRTLPSPYSISDEQDVMAKSIIGSNI
                     PDIEKITVIDLYIADSSGKQITELRSDQRVQIVNNIQNNEGYPQEFTCIIQISDSQDS
                     VILLSWIGGELSLGQNFEVSQSWTPTKSDNYKIETFVWKSLGDARPLSTSYVKSVYVE
                     "
     gene            complement(4940..8728)
                     /locus_tag="VGA92_01600"
     CDS             complement(4940..8728)
                     /locus_tag="VGA92_01600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HEX9845141.1"
                     /translation="MSVQTVKAIDGIKFSVWSPTEIRKYSVAEITAPETYDEDGMAVQ
                     GGLMDGRLGTLEPGQKCLTCGNTAARCPGHFGHIELAEPVLHIAFIDNIHKLLLATCR
                     SCSRLKLPQEELDIFGQIRKKEAAYTIISQKRIPEEILDKAKKAKECPHCGKPQYDLI
                     FTKPTIFIEKTEIGEHRLLPITIRERFTQIRDEDLKLLGYDSNTARPEWFILQVLPVP
                     PVTVRPSIILETGIRSEDDLTHKMVDIIRVNQRLKESKDAGTPPLIVQDLVDLLQYHT
                     TTYFDNEVSGIPQAHHRSGRPLKTLTQRLKGKEGRFRGSLSGKRVDFSSRTVISPDPN
                     LDLSEVGVPEAVAKKLTIPEIVTDWNIERLRELVINGPDKFPGVNYIVRPDGVKIRLD
                     FVEDRTTIAENLEVGYLVERHLSDGDIVMFNRQPSLHQMSIMAHYVKVLPGKTFRLHP
                     SVCPPYNADFDGDEMNLHVPQSEEARAEAILLMRVQDQLISPRYGGPIIGGLRDFITG
                     SYLLTKDDTVLTPQEFANYAMLGGHEGTFPEPASKGKGGPLYTGKQLFSLFLPTDFNF
                     VITSKWSKGTKGPQKDVVIKNGQLISGVIDKASIGAEEPESVLHRIAKDYGNAAAKQF
                     LNSVLIIAKQFITHYGFSYGYADLELPDKVKEGISEGIEESYNTVYDFISQAKKGTLK
                     LTRGLSAEEALEAYIVNELSKARDKAGNTADQQLDKNNAARIMATTGARGSSLNIGQM
                     AGALGQQSIRGNRLNKGYNNRALPHFKENDDNPDAHGFVKSNYREGLSTIEFFFHAMG
                     GREGLVDTAVRTQQSGYMQRRLVNALEHIKLEYDGTVRDPHGHIIQFLYGEDGIDVAK
                     SDHGEAFNINRLIESETIVDAGKKATKEEAEDLLKKYTKVFNPRLTMLVEEGVIHSKL
                     SRDGIDKVLKKALDLYYKAKAEPGQAVGIVTAQSIGEPGTQMTLRTFHFAGIRERNVT
                     LGLPRLIELVDARKKPVTPTMDIYLEEKYKKSREKAIEVARNTLQTKISALIENVETD
                     YSSQITLEISSVALNQRGCTLEEVETALQSNKKFKIETSGHTITLNLVEVADAPTVIA
                     IRNKVLNAIVKGVPDITRVTVAQKDEEWVIQTTGSNLAKVLEIDGIDKRNVRTNNVFE
                     IAATLGIEAARNALINELNTTLEDQGLEVDSRYIMLVADMMCSRGYLQQIGRHGIAGT
                     KDSVLARAAFEITVPTIAQAALTGEVEELKGVTENVIVGSNIPVGSGTVDLYMQISKN
                     "
     gene            complement(8728..12075)
                     /locus_tag="VGA92_01605"
     CDS             complement(8728..12075)
                     /locus_tag="VGA92_01605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HEX9845142.1"
                     /translation="MPDSSNKRWPIIQDLLKREGIARQHLNSFDEFLERGLQSIIDEV
                     AQIEIENAEYPYKIQLGKVKLQQPRMMELDGSITHITPAEARLRNVSYSAPIMMEASV
                     VEDGKILESRFVHIGDIPVMVRSNACILHNFSTQKLIEHGEDPNDPGGYFIINGSERV
                     IVGLEDLSYNKIIVDKEKVGGNDVFKAKVYSSIVGYRAKLELVMKNDGLIVARIPGSP
                     VDIPVVTLMRALGLEADREIAAAVALVELIQNELEASFEKAGEVQTTKDAIVYISKRI
                     APGMLEEFQIKRAETLLDWGLLPHLGKHQENRREKAQFLGEAACKLIELKLGWISPDD
                     KDHYGNKVIKFAGQMLADLFRTAFRNLVRDMKYQLERSGQKRGINAVAAAIRPGIVSD
                     KLNNAIATGNWGRGRVGVTQLLDRTNYLSTISHLRRIQSPLSRTQPNFEARDLHPTHF
                     GRICPSETPEGSNCGLVKNLALSAIISVNVSPEEIIEKLYDLGSVHFSDAKEDLKRDG
                     TRVFVDGRLVGYYKDGEKLVESLRDLRRNSKIHPHVAISFYKPSTEGATKRLYVNCNA
                     GRVLRPLVVITDGKSLLTQELIEKVSKKLMSWNDLLRMGVIELIDANEEENCYITIGD
                     KDTKKFTHLEIFPPAILGAGASIIPYPEHNQSPRNTYESAMAKQSLGFSTPMMNTSTY
                     VRQHFMWYPQTPIVTTKAMNLLGLEDRPAGQNCVVAVLPFDGYNIEDAIVISKAAVDR
                     GLGRTFFYRIYEAEAKQYPGGMRDNFEIPNSEDNIRGYKGEKAYRLLEEDGVIATESN
                     VTGGDILIGKSSPPRFMEEYREFESKGPYRRDTSIGVRPSETGVVDTVVMTQSNEGGK
                     MYKIRVRDMRIPEIGDKFASRHGQKGVVGILAKYEDLPYSAEGVIPDVLINPHAFPSR
                     MTVGMFMESISGKAAAIRGSKFDGSAFVGEKMDEIKAALEHSDFKYSGKETLYDGRTG
                     KPFPVEVFMGVVYYQKLHHMVADKIHARARGQVQMLTKQPTEGRARGGGLRFGEMERD
                     CLIAYGASMILKDRLLDESDKAEIFVCERCGLVAYHDVKQRKYVCRVCGDKAKVSSVS
                     VAYAFKLLLQEMQSLNVAPRLMIKERV"
     gene            complement(12076..12327)
                     /locus_tag="VGA92_01610"
     CDS             complement(12076..12327)
                     /locus_tag="VGA92_01610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HEX9845143.1"
                     /translation="MATKKSHVLVPDHVYVPKHEIISKKEAEEFLTKFNCKPTEMPLI
                     FANDPAIIGLGVKPGDMIKITRKSLTAGESTYYRYVVEV"
     gene            12508..13344
                     /locus_tag="VGA92_01615"
     CDS             12508..13344
                     /locus_tag="VGA92_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HEX9845144.1"
                     /translation="MSTLYAAIALFAILATGVSIVPALAQVFEDAITVATDQDSYSDG
                     DIITITGLVRERLSGYPVTLQVIAANGNLVTVKQLDVSDDKTYGIDIAAGGSLWRSAG
                     TYTIKVLYGTETRTAETTFEFSGSTGGPTPAGKSVAVEGTDFLVSYSIKGGKVISIVP
                     SLPDKSLIITIETTNDGELTVTLPRGLIDAKLGPDGMSGEDDSFFVLVDGAEVDFEET
                     TTSEDRTLTIQFEDGTTEIEIIGTFVIPEFGAMAAIILAIAIVSIIAVSARTRLSILP
                     RY"
BASE COUNT         3208 a         2932 c         2680 g         4559 t
ORIGIN      
        1 ggtggtggag gtggtggagg tggtggtgtg ggcgtggcac tgtcagagcc accgtcacct
       61 gctgctactc cagagcctat ccattgacca cattgttgac gatagtcgcc aaccatctca
      121 acgaatttgt ttgccatatc atctttagtg taaaaattaa attcagaccc acacattcct
      181 cccattgtgt caattccagt cattccagaa aatccagacg aggtgcttgt ttcagaagac
      241 ttgcctccgc tatttgcgct gctgcatttg tcagatattt cttcgccatc ttcttctgtc
      301 accatatctt catatgcgcc tgattcccag ttatttggct gcgtttttgt gtcttcaatg
      361 tcatgcgttg cccacgtgcc ggaaccgcag tcaattagca tttcatcgaa tctattcagt
      421 tcagtggact cgtagattgc gtctatatcc tccattatcg acttgccaat cgtttggtca
      481 taaaatgaca aaagtgacaa catgtctgcg cacctgatgg tcccaaagtc gtttgctttg
      541 cacaacactt tgacattaga gttgtaattt gaaattgatt ttacgagatt tgttgtatca
      601 ctatataccc ttgttgagag tgcttgctgt tttgaggaat ctagtttgta agttttagac
      661 agcgtaaatg tttctgttgc tcttttttcg actagctgat agcacaataa tttcttttct
      721 tttggtaatg aatcacagct cagttgaagt agttttactg aaacgcattt gtatccattc
      781 cacatggttg cactgggaca cacaagattt gcttgcttta ctttcatcgg cgtaccatct
      841 ttgattattt tgagagttgt agtgtctgga aatacattaa cattgagcac gtttgttctg
      901 tcaactagtg caacaaacct atccctgtca atattgctga ttcttatatt ccccgccaaa
      961 tttgctgaat cgattttcat tgttgaggtt tgttttgagc ccacctgtgt tgttggaacg
     1021 gccagcgagt ttaccttgcc cctatcaatt atggtgcttg ttgagaaatc ctttatcatt
     1081 acgtttccag ttgtcaatat gcctgtgccc tgttgtgtga caccagaaat ctggaagcgg
     1141 ctttgaggaa ttaccgtgga ctcgtcaaat tccaaaaacg acagaaggta gtagccgatt
     1201 ttttctttga ttgagagttt ttcatcaggc ccgcagagcc tgtcaccaca gacctgttcc
     1261 tcattgaatg gttggtttgc agtacccttg gctgtcttag caaatgcgtc ttctgttgca
     1321 attccagatg ctaaaagaac actagatata atgatgacca gtttaattgc ggcattcact
     1381 gaagaataag taagatttta ttataaaaat atgatcttat tgtgcatcac tttaaaaaat
     1441 catataaaaa gaactgagcc actgtaagtg gcaatagatg taaatatatt ttgaaaaaat
     1501 aatccatatt gatgcaaaaa attttaactt tttaatattg taagatgttg tgtttttacg
     1561 catcctacaa ttccaaagtg ttaaatttta ttgcaaacat agtgtcataa ttcgccacaa
     1621 ttggtgctgt aaagtccgtg tcggtttagg gaattacaaa aaatcatgca atattgagaa
     1681 aatttcagaa tatgaaaata cagggctacc aactagaact gccgagcctg cttcgggctc
     1741 tactcttatc ttgatgcttg cgctattctt tctagctcgt tctttttctt aacagacggc
     1801 gcacctgcat cattagttga tgccgaaatt agatgctctg caagatattc ttccattgct
     1861 tttggatttg aaaatgagga ttcctttacc ccgtctgcaa taaatcgcaa tgccaagtcc
     1921 acccttctta gtggagacac atcaactgat acgtggtatg cagtaccacc gtacactatt
     1981 cttgtcgtgt cttcgtttgg tgatgaatgt tctattgcac ggactagaac ctctactgga
     2041 tttttgccag tctttagatt aatgatttca aatgctgcct tgactgtatt aattgatcga
     2101 gtctttttgc cacccatcct acctgtgttt tttgcatatt tttttccaaa gtgcataagc
     2161 ttgtttgcaa gcctctcaac aatgtttacg tcggctttgt tgaatcgctt taatgaagaa
     2221 cggccaaagg tcagcggcat tattgccttt ttgagtgaga ttacactctt tagacccgga
     2281 tcctttattt caatgccgtc aagatcccat tttctaaata acaacaggtt tggtgagtct
     2341 gccatttttc tatctccttg gtttttcttt tctgcccctt acgagctcac gcaaagatgt
     2401 tccatttact ttgaaaactt tgaatcttac tcccggaata tcacccatcg ctccacccat
     2461 tgatgcaccc attccttcga gatgaacctc gtcgtgttcg tcaataaagt tcatcgcgcc
     2521 gtctcttggc aaaaacgcag taaccgtttt gccgtttttg attagctgaa cgcgaacgca
     2581 ctttcttact gcagagtttg gctgttttgc ctcaactccc actttttcca aaacaatccc
     2641 ccttgcctgc ggagaaccgc caagtggact tgcttttttg tctagtccaa gcatgcgtcg
     2701 cttgtacacg ccaattgccc agcgttgttt ttttcgcttt gtttttagaa ctctgcctgc
     2761 gaataaccct aaaggagact ttgccatacc atcactctgt aaatttatct ggactgttaa
     2821 ttaaaacgct tgtaatttca aaatatcttc ttgccaaaag cctagccttt tctgcattgc
     2881 ggccctctcg ccccacaaca attcctttct tttttggatc gactagcaca attgcctgag
     2941 ttgtcccatc aagccttttg tttaatttga cttccgttac aagttttgag tttagcatgt
     3001 ttttcaaaaa ctctattggg tcgtcggaat attctacaag ctcaacgttt ttcttgataa
     3061 tattttgcag attcttaatg tgtgagccgc ctttgcctat tgcaagaccc atctttcctt
     3121 catttacaac aaaaatcaca cggttttgtt tttcatcttc cacacaatca cgagctgacg
     3181 cgcctgttac attttggaaa agcgagatta atcgcatttg atcagttgtg agtttgattg
     3241 tttgtggcat ttttgctaat tatctcgaag ccctgagggt ctcatttaaa ctttgagaga
     3301 gttgtgatgg tgtcatgttt attgtacctc tgattctttt agtattgcct tgatgttaga
     3361 gtcaggaatt gatgtcaatg atgctgctga aactctaaat tgcaatccgc aaacttttcc
     3421 aagtgccaca gaagaaccat caaactgcag tgttgtaact ttttggtctt tggaactttc
     3481 aattttatct atggattctt ttgatgcgga tttggaaact acaactagct ttgcgttctt
     3541 aattgattgc agcacctgct ttacgcccaa tacgcatttg ctttcctgta gggcgtcctt
     3601 tagtgctttt tcgagtaatt ttcccattat atcacgtgta atgacggttc aaaacctccc
     3661 ttatagattt atcactttgt gatttgaaca aaatatcaac ggttcaaatt gaaaaattat
     3721 tcgacgtaaa cagatttgac gtaacttgta ctaagtggtc gtgcatcacc tagtgatttc
     3781 cacacaaaag tttctatttt gtaattatcg ctttttgtcg gagtccacga ctgtgaaacc
     3841 tcaaagtttt gtcctagcga caactctcct cctatccaag aaagcaaaat cacgctatct
     3901 tgcgagtcgc tgatttgtat aatgcaggta aattcttgtg ggtaaccctc attgttctga
     3961 atgttgttta ctatctgaac ccgttgatct gatctcagct cagttatttg ttttccactt
     4021 gaatccgcaa tgtaaagatc aataacagta attttttcaa tatctggaat gtttgatccg
     4081 ataattgatt ttgccattac gtcttgttcg tctgatatgc tgtatggcga tggaagtgtt
     4141 ctgtcctcat atcttgcagt aatggtattg cctggaattg caagaagcct gtttccgcta
     4201 gagtcgtttg tttgtgtcaa aaccaccaga gcttcaaaaa ttccagagtc ctcgcctgtt
     4261 tctgttgcgg caactgaaat tccagcggaa tcagaatctg atgatacatc tacattgacc
     4321 tgatctaaag actctggatt tagattcatg tctgcatccc ttacttggat gactacctga
     4381 tcagttacaa gatagtttgg attagagaat tcaatttctg caaaattcca gcttactttg
     4441 actgatttgg taagcaccac gccatcagca aactcaaatg aaatcgtaat tccgtcatcc
     4501 ctttttgtct caagcagtcc acttgttggg cccaccccca tcgttcgcgg ttgtgtatct
     4561 gattgcccgt caccgtcagc atcatgcaga aacccagtca ggacaacctc gcctgtaaag
     4621 attccgctgt ttggcgcagt ttctgtaagc ctgtatggct caagggagtg gctcgatgta
     4681 gaaattttta ctgggtgcga tgcatcatct ccaatcgaat caatgccgta cttgttttca
     4741 ttccagcttg gcgcagctat tgttatgcga attttgtctg tccacgagta cacttctttg
     4801 tcagtgtaga ttggcgcata cggtttgtta tagtccggaa tttgctctgc gtatgctcca
     4861 attacaagaa atactacgcc tattagtgct aacaatcgaa tttagtttag ttgttgtgat
     4921 ttttgaagct agctgagaac tagttctttg agatctgcat gtacaggtcc actgttcctg
     4981 aaccaaccgg aatgttgctt ccaacaatta cgttttcagt aacacctttg agctcctcta
     5041 cttcgcccgt cagagcggcc tgcgcaattg tcgggacggt aatttcgaat gcggctcttg
     5101 caagaacgct gtctttggtt cctgcgattc cgtgacggcc aatttgttgc aggtatccac
     5161 gagagcacat catgtctgca acaagcatga tatatcttga gtcaacttct agtccttggt
     5221 cttctagtgt tgtgtttagt tcgttgatta gtgcatttct tgctgcctcg attccaagtg
     5281 ttgcagcaat ctcaaagacg ttgtttgttc gaacgtttct tttgtcgatt ccatcaattt
     5341 caagcacttt agcaagattt gagccagttg tctgaatgac ccattcttcg tctttttgcg
     5401 cgacggtgac ccttgtgatg tctgggactc ctttcactat tgcgtttagt accttgtttc
     5461 ttattgcaat gactgttggc gcatctgcca cctctacaag attcagtgtt attgtatgtc
     5521 cactagtctc gattttgaat ttcttgtttg actggagtgc tgtctctact tcctcaagtg
     5581 tacagcctct ctggtttagt gcaacagagc tgatctccaa tgttatttgt gatgaataat
     5641 cggtttcaac attttctatt agtgctgaaa ttttggtttg tagtgtattt cttgcaacct
     5701 cgattgcctt ttctcttgat tttttgtatt tttcctccaa gtagatatcc atagttggag
     5761 taacaggttt ctttcttgcg tcaactagtt caattagtct tggaaggcca agtgttacgt
     5821 ttcgttccct gattcctgca aagtggaatg tcctaagtgt catctgagtt cctggctcgc
     5881 caatggactg ggctgtgaca attcccactg cctgtcctgg ctctgccttt gccttgtagt
     5941 aaagatcaag tgcttttttg agaactttgt caattccgtc tctgcttagc tttgagtgta
     6001 tgactccctc ctcaactagc attgtaagtc ttggattgaa tactttggtg tattttttga
     6061 gcaggtcttc tgcttcttcc tttgttgcct ttttgccggc atctacaatt gtctcagatt
     6121 cgattagtct gttaatgtta aatgcctcgc cgtggtcgct ttttgcaacg tcgatgccat
     6181 cctcaccata caggaactgg attatgtggc cgtgtggatc acgtaccgtt ccatcatact
     6241 ctagcttaat gtgctcaagt gcgttgacta gtcttctctg catgtaacca ctttgctggg
     6301 ttctgactgc ggtgtcgact aggccttctc gtccacccat tgcgtggaag aagaattcta
     6361 ttgttgagag gccctccctg tagtttgact ttacaaatcc atgggcgtct gggttatcat
     6421 cattttcttt aaagtgcggc aaagccctgt tgttgtaacc cttgttaagc ctgttaccac
     6481 gaattgattg ttgtcccaac gcacctgcca tctgaccgat gtttagtgat gagcccctag
     6541 ccccagtagt tgccatgatt cttgccgcat tgtttttgtc aagctgttgg tctgctgtgt
     6601 ttcctgcctt gtctcttgcc tttgagagct catttacaat atatgcctca agtgcctctt
     6661 cagcagaaag acccctagtg agtttcagtg ttcccttctt tgcctgcgat atgaaatcat
     6721 aaactgtatt gtatgattcc tcgattccct ctgagatgcc ttctttgact ttgtctggca
     6781 gctccaagtc ggcatatccg tagctgaatc catagtgagt gatgaattgt tttgcaatta
     6841 tcaaaacgga gtttaggaat tgttttgcag ctgcatttcc atagtctttt gcaattctgt
     6901 gtaaaacgct ttctggttct tccgcaccaa ttgatgcctt gtcaattaca ccgctaatca
     6961 gctgaccgtt tttgattaca acgtcttttt gtggtccctt tgttcccttg gaccacttgg
     7021 atgttatgac aaaattaaag tcagttggta agaatagtga gaacagctgc ttgccagtgt
     7081 acagtggtcc cccttttcct ttgctcgcag gttctggaaa cgtcccttcg tgtcctccga
     7141 gcattgcata gtttgcaaat tcctgtgggg taagaaccgt gtcgtctttt gttagaagat
     7201 aggagccagt gataaagtcc cgcagccctc cgataattgg gccaccgtat cttggtgaaa
     7261 tgagttggtc ttgtactctc atcagtaaaa ttgcctctgc tcttgcctcc tcgctttgcg
     7321 gaacgtgtag attcatctca tccccatcaa agtctgcgtt gtatggcgga catactgacg
     7381 gatgcagtct gaaggttttt ccaggaagca ctttgacata gtgagccata attgacattt
     7441 ggtgcaatga tggctgcctg ttaaacatga caatgtcgcc atccgacaaa tgtctttcaa
     7501 caagatatcc tacttcgagg ttttctgcta tcgttgtgcg atcctctaca aaatccagtc
     7561 tgatttttac tccgtctggc cttacaatgt agttgacacc tggaaacttg tcaggaccat
     7621 ttatgaccag ctctctcaat ctctcaatat tccagtctgt tacaatttct gggatcgtca
     7681 gcttttttgc aactgcttct ggtacgccga cttcggatag atccaagttt ggatcaggcg
     7741 agatgaccgt tctgctagaa aagtctactc ttttgccaga aagtgatcct ctgaatcttc
     7801 cctcttttcc tttgagtctt tgtgtaagtg tctttagtgg tctgcctgag cggtggtgag
     7861 cctgtgggat gcctgatact tcgttatcaa agtatgtggt ggtgtgatat tgtaatagat
     7921 caactaggtc ttgaacaata agtggtgggg ttccggcatc tttgctttct ttgagtctct
     7981 ggtttaccct tatgatgtcg accattttgt gagtaagatc atcttcggac ctaatgccag
     8041 tctctaaaat tattgatggt cttactgtga ctggtggaac aggtaggact tggagaatga
     8101 accattctgg tcttgctgtg tttgagtcat atcccaaaag cttgaggtct tcgtctcgga
     8161 tttgggtaaa tctttcacgt atggtgattg gaagtagcct gtgttcgcca atttcggttt
     8221 tctcaataaa gattgttggc tttgtaaaaa tcaaatcata ctgcggcttg ccacagtgtg
     8281 ggcattcttt tgcctttttt gccttgtcta ggatttcctc tgggattctt ttttgtgaaa
     8341 ttatggtgta tgctgcttct ttctttctga tttgaccaaa aatatcgagt tcctcttgcg
     8401 gaagctttag tcttgagcag gaacgacacg ttgcaagcag taacttgtga atattgtcaa
     8461 taaatgcaat gtgcaagacg ggctctgcta gctcgatatg gccaaagtgc cccggacatc
     8521 ttgctgcagt gtttccgcat gtaagacatt tttgtcctgg ctccagcgtt ccaagtcttc
     8581 cgtccatcag acccccttgg actgccattc cgtcctcatc atatgtttca ggtgcggtga
     8641 tttcagcaac agaatacttt cttatctctg ttggagacca aaccgaaaac ttgattccgt
     8701 caattgcttt tactgtttga accgacatta cactctctcc tttatcatta gtcttggtgc
     8761 cacattgaga ctttgcattt cttgaagcag tagcttaaat gcataagcaa cagaaactga
     8821 ggatactttt gctttgtcgc cacaaactcg gcacacatat tttctttgtt taacatcatg
     8881 atatgctaca agaccgcatc tttcgcaaac aaagatttca gctttgtctg attcatcaag
     8941 taatctgtct ttgagaatca tcgatgcacc atatgcgata agacagtctc tttccatttc
     9001 accaaatctt aggcctccgc cccttgctct tccctcagtt ggttgttttg tgagcatttg
     9061 gacttggcct cttgctcttg catgaatttt gtctgcaacc atgtggtgaa gtttctgata
     9121 atagactaca cccataaaga cctcaactgg gaatggtttt cccgttctgc cgtcatacag
     9181 tgtttctttt ccagaatact tgaagtcgct gtgttctagt gcagccttga tttcatccat
     9241 tttttcgcca acaaatgcag agccgtcgaa tttacttccg cgaattgctg cagccttgcc
     9301 tgaaatcgac tccatgaaca ttcccactgt catacgtgat gggaacgcgt gtgggttgat
     9361 caaaacatct ggaataactc cttctgctga atatggcaga tcctcatatt ttgctaaaat
     9421 tccaactacg cctttttgtc cgtgtcttga tgcgaacttg tctccgattt ctggaattct
     9481 catatccctt actctaatct tgtacatttt tccgccctcg tttgattgtg tcattactac
     9541 agtatctaca accccagtct cagacggtct tacacctatt gatgtgtctc gcctgtatgg
     9601 accctttgac tcaaattctc tgtattcttc catgaatctt ggagggctgc tctttccaat
     9661 caaaatgtct cccccagtga cattagactc agtagctatt acgccgtctt cttctagtag
     9721 acgatatgct ttttctcctt tgtaccctcg aatgttgtct tcagagtttg gtatctcaaa
     9781 gttgtctcgc attccacccg gatactgttt tgcttctgcc tcatagattc tataaaagaa
     9841 agttctgcca aggcccctgt ctactgccgc cttgcttatc acaatcgcat cttcaatgtt
     9901 gtaaccatca aaaggcaaga cagcgacaac acagttttgt cctgcaggcc tgtcttcgag
     9961 tccaagtagg ttcattgcct ttgttgtaac gattggtgtc tgtggatacc acataaagtg
    10021 ctgtcttaca taggtgctcg tgttcatcat cggagtcgaa aagccgaggc tttgctttgc
    10081 cattgcagac tcgtatgtgt ttcttggaga ctggttatgt tccggatatg gaattattga
    10141 cgcgcctgcg cccaagattg caggtgggaa tatttccagg tgggtgaatt tttttgtatc
    10201 cttatcgcca attgtgatgt agcagttttc ttcctcgttt gcgtcgatta gctcaattac
    10261 gcccattcta agaaggtcat tccatgacat tagtttcttt gaaacctttt cgatcagttc
    10321 ttgcgttagc agtgacttgc cgtcagttat gacaactagt ggacgtaaaa cacggcccgc
    10381 gttgcaattc acatagagac gttttgtcgc accctctgtg cttggcttgt aaaatgatat
    10441 tgctacgtgc ggatgaattt tggagtttcg tctcagatca cgcaatgact cgactagctt
    10501 ttcgccatct ttgtaatagc caactagtct gccatctaca aagacgcgcg ttccgtctcg
    10561 ctttagatcc tcttttgcat cgctaaagtg aacagatcct aggtcataca gtttttcaat
    10621 aatttcttct ggtgaaacgt taactgaaat gattgcagat agtgcgagat tttttacaag
    10681 accgcagttg gagccctctg gtgtttcact tggacagatt cttccaaagt gtgttggatg
    10741 aagatctctt gcttcaaagt ttggttgggt cctcgaaagt ggggactgga ttcttcttag
    10801 atggctaatt gttgaaagat agtttgttct atcaagaagt tgtgttacac caactctacc
    10861 ccttccccag tttcctgtag caattgcgtt gttgagctta tctgatacaa ttccaggtcg
    10921 tattgctgct gccaccgcgt tgattccacg tttttgtcct gatctttcaa gttggtattt
    10981 catatcacgc accaagtttc taaacgcagt tctaaacaag tcagcaagca tctgtcctgc
    11041 aaatttgatt accttgtttc cataatggtc tttatcgtcc ggtgaaatcc agcctagctt
    11101 tagttcaatg agcttacatg ccgcctctcc tagaaattgt gctttttccc ttctgttttc
    11161 ttggtgtttg cccaagtgtg gtaaaagccc ccaatccaaa agcgtctctg ctcttttgat
    11221 ttggaattct tctagcattc caggagcaat tcttttgctg atgtagacaa ttgcgtcctt
    11281 tgttgtttgg acttcgcctg ccttttcaaa tgacgcctct agctcgtttt gtattaattc
    11341 tacaagtgcg actgcagctg caatttccct gtctgcctca agacctaggg cgcgcatcaa
    11401 tgttacaact ggaatgtcaa caggagatcc gggaattcgt gcaacaatga gaccgtcgtt
    11461 tttcattaca agttctagtt ttgcacgata cccaacaata gacgagtaga ctttagcctt
    11521 gaagacatcg ttgcctccaa ctttttcctt atctacaata attttgttgt atgaaagatc
    11581 ttctagtcct acaattactc tttctgatcc attaatgata aagtatccgc caggatcatt
    11641 tgggtcttca ccgtgctcaa ttaatttttg tgtagaaaaa ttatgcaaga tgcaagcgtt
    11701 tgatctaacc attactggta tgtcgccaat gtggacaaat cttgattcga gaatttttcc
    11761 atcctctacg acgcttgcct ccatcataat tggtgcagag tatgagacgt ttcttagtcg
    11821 agcttcggct ggggtgatgt gtgtaatgga gccgtctagt tccatcattc ttggttgttg
    11881 aagttttact ttgcctagct gaattttgta tggatattcg gcattctcta tttcgatttg
    11941 tgctacctca tcgatgatgc tttgtaatcc acgctctaag aattcatcaa aagagtttag
    12001 atgttgtctt gcaatgcctt ctctttttag caggtcttga ataattggcc agcgtttgtt
    12061 agaagaatct ggcatttaga cttccaccac atatctgtaa taggtacttt cgccagcagt
    12121 taggcttttt cttgtaattt ttatcatgtc tcctggtttt actcctagtc cgattattgc
    12181 tgggtcgttt gcaaaaatta atggcatctc agttggcttg cagttgaatt tcgttaggaa
    12241 ttcttcagct tccttttttg atatgatttc atgttttggt acgtaaacat gatctggaac
    12301 aagtacgtga cttttcttag ttgccaactt tacgatcacc ttggatagca ttagctaaat
    12361 atgaaaaatt accacacaaa ctgaaaaaac tccaaaagtg gtttatatat atgtaatcaa
    12421 gatctacaaa gaaatcaata ttttttgttt ttagtcaatg atttttttct taagcttaaa
    12481 tagtatttgt taacacggat aagcaagatg agtactctgt acgcagcaat agcattgttt
    12541 gcaattctgg ccacaggtgt atctatagta ccagctcttg ctcaagtatt tgaagatgca
    12601 attacagtag caactgatca agactcttac agtgatggag acatcattac aataactggt
    12661 ctagtcagag aaagactttc tgggtatcca gtgactttgc aagtaattgc agccaatgga
    12721 aacttggtta ctgttaaaca actggatgtt tcagacgaca aaacatatgg aattgacatc
    12781 gcagcaggtg gttcactatg gagatcggca ggaacataca cgataaaggt actctacgga
    12841 accgaaacaa gaacagcaga aactaccttt gagtttagcg gctctactgg tggtccaact
    12901 cccgcaggca aatcggtcgc agtagaaggc actgatttct tggtaagcta ctccataaaa
    12961 ggaggtaaag tgataagtat cgtaccaagc ttacctgaca aatcattaat cattacgatc
    13021 gagactacca atgacgggga attgactgta accttaccaa gaggcctaat tgatgcaaaa
    13081 ttaggtcctg atggcatgtc aggcgaagat gacagtttct ttgttctagt agatggtgcc
    13141 gaagtagact ttgaggaaac aacaacatct gaagatcgca ccttaaccat acagtttgaa
    13201 gatggtacaa ctgagatcga aatcattggt accttcgtaa ttccagaatt tggcgcaatg
    13261 gcagcaataa tccttgcaat agcaatcgta tcaataattg ccgtctctgc gagaacacga
    13321 cttagtatac tgccaagata ctagatcttc aacttttttc attttttaaa tatacataa
//
LOCUS       DASWKH010000018         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2994264, whole genome shotgun sequence.
ACCESSION   DASWKH010000018 DASWKH010000000
VERSION     DASWKH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2994264"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VGA92_01620"
     CDS             <1..147
                     /locus_tag="VGA92_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9845145.1"
                     /translation="AIKSANNPMKPGESGEVTFTAGAPGTYYYICTVPGHSALGMKGS
                     FIVE"
     gene            149..592
                     /locus_tag="VGA92_01625"
     CDS             149..592
                     /locus_tag="VGA92_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9845146.1"
                     /translation="MLLKYLALSSLVILYSLMFIGGYVSAAGLGLSCPDWPLCPNGIL
                     PDNDYLIEWIHRFVAATTGMLIIATTIGVWLNKHTDKKIKLTSTLAAIFVVTQITLGA
                     LVIDLKLHAVLVAIHLGIGILLFAMTLLTTIFVFRLAKETIQTKA"
     gene            complement(582..1091)
                     /locus_tag="VGA92_01630"
     CDS             complement(582..1091)
                     /locus_tag="VGA92_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845147.1"
                     /translation="MLFRLALFAFLIVSSIPIQQASAHALFNSAESTIGNNRVQIATD
                     PEIPTVGEKSRILLHVTDLDLNDVNRFTMGIRIFYNDIQIDGVPPQSIDGAFWDFDYT
                     FKTSGNHIFRVDLYDPDLGVVTHTFNLSTQNPFGYIFIYVIASGAIGLAVLVGYIYLP
                     KKLKKLTRP"
     gene            1185..1787
                     /locus_tag="VGA92_01635"
     CDS             1185..1787
                     /locus_tag="VGA92_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9845148.1"
                     /translation="MTTVRKSIDIKAPIDAVFTYFARPEHVSDQMTEKGVAMSVIPMD
                     IKDGMGVGTTFRIVGDFNGKRLEWDCETTEFVREQRIAAKMIKGPFKKWGITSEFAPK
                     SERVTTVTMTVNYDMPFGPLGGIINKLKFAKTAERGMESALYKVKGLLEGNGSIPVYI
                     TLDAYQKLLEEKKKMNNVPVSTVLTAILEKYNEIEAKTSN"
     gene            1837..1925
                     /locus_tag="VGA92_01640"
     tRNA            join(1837..1874,1890..1925)
                     /locus_tag="VGA92_01640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1871..1873,aa:Lys,seq:ctt)
     gene            2160..>2775
                     /locus_tag="VGA92_01645"
     CDS             2160..>2775
                     /locus_tag="VGA92_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845149.1"
                     /translation="MKVFLILVFILIGFISTANAQVTEKLLISVGLQTNENNVPVISG
                     IIKNEKGDLIGDTIIQISSVMGTAETKSGADGKFVYHMPILPSEDKFNISVKAQKEGY
                     LAGYANTSFFVQNKNQTNTESKPLDSGFKIVTSDKIKNDPIAFKILQNIELNKQKEEE
                     RQKRLQEIEAREKFIEEQRQIANQDLLADLQSMVEQFDPFNPRNV"
BASE COUNT          934 a          536 c          545 g          760 t
ORIGIN      
        1 gcaatcaagt ctgcaaacaa tccaatgaag ccgggtgaaa gcggtgaggt gacatttact
       61 gcgggagcac caggaacata ttattacatt tgtacagtgc caggtcactc tgcactggga
      121 atgaaaggta gtttcatagt agagtaactt gcttctaaaa tatctggcat tatcatcgct
      181 tgtaattctt tactctctga tgtttatcgg cggatatgtt tctgcagcgg gtcttggact
      241 atcatgtcct gactggccgc tttgcccaaa tggaatcctg cctgacaatg attatctaat
      301 agaatggatt cacagattcg ttgcggcaac tactggtatg ctgataatag ctactacgat
      361 tggcgtatgg ctaaacaagc acactgacaa aaaaatcaaa ctaacgtcga ctcttgcagc
      421 aatctttgtc gtcacacaga ttaccttggg tgcactagta attgacctaa agcttcatgc
      481 agtcctagtt gcaatacatc ttggaattgg aattttgctt tttgcaatga ctttgcttac
      541 tacgatcttt gtgtttcgac tagccaagga aacgattcag actaaggcct agtcagtttt
      601 tttagttttt ttggcaggta gatatatcca accaaaactg caaggcctat tgcgcctgac
      661 gcaataacat agatgaaaat atacccaaat ggattctgcg tgcttagatt aaacgtgtgt
      721 gtaactactc ccaaatcggg gtcgtacagg tctactctaa agatgtggtt tccagatgtt
      781 ttgaaggtat agtcaaaatc ccagaatgcg ccatctattg attggggtgg cacaccatct
      841 atctggatgt cattataaaa tatcctaatt cccatcgtaa aacgattaac atcgtttagg
      901 tcaaggtctg tgacgtgcaa caaaattcta gatttttcac caaccgttgg gatctcggga
      961 tctgttgcga tctgaaccct attgtttcca atggtggatt cggcagaatt gaaaagcgca
     1021 tgtgcactag cttgttgtat tggtattgat gagacaatca aaaacgcgaa aagcgctaat
     1081 ctaaataaca cgatctgaag actcttgcac tcaatatatg caaaatgatt tttctccgat
     1141 aaatgacaaa agctttaaat gcagactaga aaaaaagcca agcgttgact acggtcagaa
     1201 aatcaattga tatcaaagca ccaattgacg cagtttttac atatttcgca agacctgagc
     1261 atgtctctga tcagatgact gaaaaaggcg ttgccatgag cgtgattcca atggacatca
     1321 aagacggcat gggtgttgga actaccttta gaattgtcgg cgactttaac ggaaaaagac
     1381 tagaatggga ctgtgaaacg actgagtttg ttcgagagca gagaattgcc gcaaaaatga
     1441 tcaaaggacc attcaaaaaa tggggcatta catcagaatt tgcgccaaaa tcagagcgag
     1501 tcactaccgt aacaatgact gtaaactatg acatgccatt tggcccactt ggcggaataa
     1561 ttaacaaact caagtttgcc aaaactgcag agcgtggaat ggaaagcgcc ctgtataagg
     1621 taaagggatt gcttgaagga aacggctcaa ttcctgtgta tatcacatta gatgcatacc
     1681 aaaaattgct tgaagagaaa aagaagatga acaacgttcc tgtttctact gttctgactg
     1741 caattttgga aaaatacaac gaaatcgaag ccaaaacttc aaactaaatt ttcccgctca
     1801 actttaaata accactctgg cgttttttgc tctgtggggt ctatggctca gccaggtaga
     1861 gcgaaggact cttaaaatta cttggagaca tccttatgtc gtgggttcaa atcccactag
     1921 acccgcttta ctatctaatc caaaacgaca tagaatttgg taaaaaatct gggattttca
     1981 tcaaaataac atctgggagg aattagtgaa caatttcttt tatactctct caaattatct
     2041 gcaaaaaagg tgcaaatatt aaaaaatttg taaaatggtt ccaagctcca aactttaaac
     2101 aacaaaatac gaaaaaatcg attcatttct ttaaggtaaa aaacaaaaac tcgtaaaaaa
     2161 tgaaagtttt cctgattttg gtttttatac tgataggttt tatcagcaca gcaaacgctc
     2221 aggtcactga aaaactgctc attagtgttg gtcttcaaac aaatgaaaac aatgtccctg
     2281 taatttctgg tatcattaaa aacgaaaaag gtgatctcat cggagatact attattcaaa
     2341 tttcttccgt catgggcaca gcagaaacaa aatctggtgc tgatggcaaa ttcgtatatc
     2401 atatgccaat tttaccttca gaggacaaat tcaacataag tgtaaaggcg caaaaggaag
     2461 gctatctggc aggttatgca aacacctcat tttttgtgca aaataaaaat caaacaaata
     2521 cagaatcaaa acccttggat tctggcttta aaattgtaac ttcggacaag ataaaaaatg
     2581 atccaattgc ctttaaaatt ttacaaaaca ttgagctaaa caagcaaaaa gaagaagaaa
     2641 ggcaaaaaag actgcaagaa attgaggcgc gtgaaaaatt catagaagaa caacgccaaa
     2701 tagcaaacca agacctgcta gccgatcttc aatccatggt ggagcagttt gacccattca
     2761 acccacgaaa cgtat
//
LOCUS       DASWKH010000019         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_784300, whole genome shotgun sequence.
ACCESSION   DASWKH010000019 DASWKH010000000
VERSION     DASWKH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784300"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGA92_01650"
     CDS             <1..285
                     /locus_tag="VGA92_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845150.1"
                     /translation="EKDIEFTIIKNNNEGESAIIVTEETETQNTILEIESNENERTLV
                     IPISSEDNHIAIVGTKVVPEFPIGTMIIIISTISGMIIIATLKRNLFMQS"
     gene            complement(422..1621)
                     /locus_tag="VGA92_01655"
     CDS             complement(422..1621)
                     /locus_tag="VGA92_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845151.1"
                     /translation="MSLAPQELENNASRYASDAIRLDSQGARGMAVANYQKAIDSLVK
                     LIHLYPDNKLNRVYTERCRAYENRIKALQMAHGVDLEPAVDPNATPSEQKAQLEKKKD
                     EDDFDDLVMKEKPDISWKEVIGLDDAKNALRESIVYPTQRPDLFPLGWPRGILLYGPP
                     GCGKTILAAATASELDGYFIVVDAASMMSKWLGEAEKNVSKVFKLARSYVEKENKPVI
                     LFVDEVDSLLGNRSSEVGGEVRIRNQFLTEMDGINEKGKDLKLYVIGATNKPWSLDWP
                     FLRRFQKRVYVPLPTLEAREKLFELYTNPLQKDDRVRPLELAKIFDGYSASDIKDVCQ
                     GVQLMVVNELFSNPSYHEPIAGENQPQPRDLNMNDFREIMTRRKPSVSMDMIRAYYKW
                     SEQFKAL"
BASE COUNT          471 a          383 c          325 g          586 t
ORIGIN      
        1 gagaaagata ttgagttcac catcataaaa aacaacaatg agggtgagtc agcaataata
       61 gtcactgaag aaacagagac acaaaacaca attttggaaa ttgaatcaaa tgaaaatgaa
      121 agaacattag tcattccaat atcttcagag gataaccata ttgcaatagt aggcacaaag
      181 gtagttccag aatttccaat tggaaccatg ataataatta tatcgacaat atcgggtatg
      241 ataattattg ctacattaaa aagaaacttg tttatgcaaa gttaatctat cttacaaaaa
      301 tatgctcaaa cataaaaacg tcaaattgta ataaaatttt aaaaatcatt cataccgccc
      361 ttgttaaaca acgatcttca taaaatggaa actttttctc taatggtgtt ttagagtgat
      421 tttacagggc tttgaactgc tcgctccact tgtagtatgc tcggatcatg tccattgaga
      481 cacttggctt tcttcttgtc atgatctccc taaagtcatt catgttcaga tcccttggtt
      541 gcggctggtt ttctccagct attggctcat gatatgacgg attcgaaaat agttcattta
      601 ccaccatgag ctgcacgccc tggcagacat ccttaatgtc gcttgcgctg tacccatcga
      661 agatttttgc aagctccagt ggccttaccc tgtcgtcttt ttggagagga tttgtgtata
      721 actcgaataa tttttctctt gcttcaagtg ttggaagtgg aacgtaaact cttttttgga
      781 atcttcgcaa aaatggccaa tcaaggctcc atggcttgtt tgttgcacca atcacataca
      841 gttttaggtc tttgcctttt tcgtttattc catccatctc tgttaagaac tgatttctta
      901 ttctaacctc tcctcccact tcggagcttc tgtttcctaa aagtgagtcg acctcatcga
      961 caaataaaat cacaggtttg ttttcttttt ctacataact acgtgcaagc ttgaaaacct
     1021 ttgagacatt tttttctgcc tctccaagcc atttgctcat cattgatgct gcatcaacta
     1081 cgataaagta accatcaagt tcacttgctg ttgccgcagc caagattgtc ttcccacatc
     1141 ctggtggacc ataaagtaga attccgcgcg gccacccaag tgggaacagg tctggcctct
     1201 gtgtcggata gacgattgat tcacgtaagg cattctttgc gtcatcaagg ccgattacct
     1261 ccttccaaga gatatctggc ttttctttca tcactagatc atcaaagtcg tcttcgtcct
     1321 ttttcttttc aagctgggct ttttgttcag aaggtgttgc gtttggatca actgctggct
     1381 ccaagtccac tccgtgcgcc atttgcagcg ccttgattct attctcataa gccctgcagc
     1441 gttccgtgta aaccctgttt agcttgttat ccggatacaa atgaattagt tttactagcg
     1501 aatctatcgc tttttgataa tttgcaactg ccatgcctct tgcgccttgc gaatcaagcc
     1561 taattgcgtc agatgcatac ctgcttgcat tattttctag ttcctgcggt gccaaactca
     1621 tcttaaacta cctgctcgtt ttggactgga ttattttgtt ggtatccctg tgggtaaaca
     1681 ccagtagcag atttggttaa atttattgta ccttgatttg acgccaaggt ttgcggttgt
     1741 ttttttgtat catctattga gaggt
//
LOCUS       DASWKH010000020         3305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_409573, whole genome shotgun sequence.
ACCESSION   DASWKH010000020 DASWKH010000000
VERSION     DASWKH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409573"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VGA92_01660"
     CDS             <1..866
                     /locus_tag="VGA92_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX9845152.1"
                     /translation="DESYTLSLDQIEARVKTAWEMFGIRQVLIQGGHNPNLGIEYYED
                     AFRMIRKKYPQVGVHGLSASEIDMIAKIEKTSTKEVLLRLKEAGLQSVPGAGAEILVD
                     SVKEIISPKKISSDEWIRIMGEAHAIGLPASATMMYGHVETIEDIAEHFRKTVQLQEK
                     TGGFMAFIPWSFEPNNTLMQKEKTVLYGVGGIKLLKMIAISRLVFDGLIPHIQSSWLT
                     NGVGMAQIALQYGADDFGGTLIGEEVVSCTGARSTELTGQKIIDAIHQIGYDVEERDN
                     FYNPVKMCYMP"
     gene            complement(854..2296)
                     /locus_tag="VGA92_01665"
     CDS             complement(854..2296)
                     /locus_tag="VGA92_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX9845153.1"
                     /translation="MPIEDTSEFIKALEDAGELRRVKTQVDADLEIAEILRRTMYANG
                     PAILFENVKNYDMPVLGNAFGSLKRLEIGLQTSDFTEIGQRIVDMTKMEIPSGILNKI
                     KKLPELSKMGEVFPKLEGSGPVTEVFDDSPSFNKIPILKTWYKDAGRFITLGLVATKH
                     PETGVRNLGVYRMQILDERTALMHWQKHKRGAHHHDISKEQGKKIEAAVIIGGDPATV
                     FSAVAPVPEGLDKYLFAGITRQKGIKTVKCRTIDLEVPANAELVLEGYVDPSDIRDEG
                     PFGDHTGYYTPKEPYPTFTLTGIMRRKKPIYLTTIVGKPILEDAYIGKVIERSFLPLI
                     KMFQPEVVDFAMPAAGWFQGMAIISIKKRYPGQAKKVMMGLWGLGQLALTKMFVVVDD
                     DVNVHSMDDVIWAITTRADPARDTIIINNAPTDTLDPASPLVNLGSKLGIDATQKTKE
                     EGFTREIQEKVAVDKDTKEFVDSKWSSYGI"
     gene            2399..2629
                     /locus_tag="VGA92_01670"
     CDS             2399..2629
                     /locus_tag="VGA92_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845154.1"
                     /translation="MSAEKCVVCKGKGTIELLATQCPFCNGTGEYNKSGDSYLKSHIC
                     QCIFLDRKTCPLCGKKCHHDTPNKPKILIGPV"
     gene            complement(2642..3019)
                     /locus_tag="VGA92_01675"
     CDS             complement(2642..3019)
                     /locus_tag="VGA92_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845155.1"
                     /translation="MDDFEREFPEFDWKNTPAYKHPGGKDCPCPKHEYIREQINFAKT
                     VNQTSKEPTKVTLKFCPEHYKVYMDEVIPSMPLKYKILTKVALKIGAIQLEKLTHMQS
                     DLCFYCKFGSGGHGKKSELPPIQ"
     gene            3118..>3305
                     /locus_tag="VGA92_01680"
     CDS             3118..>3305
                     /locus_tag="VGA92_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sialidase"
                     /protein_id="HEX9845156.1"
                     /translation="MSSGKLTKKDLEAKIAELEAKLEKLSAQIEAKPVQTKTPPPPQV
                     QTPPKPQTQTPPPKPAEEA"
BASE COUNT          874 a          738 c          748 g          945 t
ORIGIN      
        1 atgacgagtc atacaccctg agccttgatc agatcgaagc aagagtaaag actgcatggg
       61 agatgtttgg gattcgccag gttttgatcc agggaggcca caacccaaac ctcggaatag
      121 aatactacga ggatgcgttt aggatgatcc gaaaaaaata cccccaagtt ggagttcacg
      181 gcctttcagc atccgaaatt gacatgattg caaaaattga aaaaacatca acaaaagaag
      241 tcttgctgcg actaaaggag gcgggcctcc agtcggtacc gggtgctgga gctgaaattc
      301 tagtcgactc tgtaaaagaa ataatcagcc caaaaaagat ctcaagtgat gagtggatta
      361 gaattatggg cgaagcacac gcaattggac ttccggcatc agctactatg atgtatggac
      421 acgttgaaac cattgaagac attgctgagc attttcgcaa aactgtacaa cttcaggaaa
      481 aaacgggcgg atttatggcg ttcattccgt ggagctttga gccaaacaac acgctcatgc
      541 aaaaagaaaa aacagttctt tatggcgttg ggggaataaa gctgctaaaa atgattgcaa
      601 tctcgaggct tgtctttgac ggcctaattc cgcacatcca gtcatcatgg cttacaaatg
      661 gggtcggcat ggcgcaaatt gcgctacagt atggcgcaga cgattttggc ggcacgttaa
      721 ttggcgagga ggtggtctct tgcacaggtg cccgctcaac tgagcttact ggccagaaaa
      781 taattgacgc aattcatcaa ataggatatg acgtagaaga acgggacaac ttttacaatc
      841 ctgtcaagat gtgctatatg ccgtagctgg accacttgga gtcaacaaac tcttttgtgt
      901 ctttgtcgac tgcgactttt tcctgaatct ctcgcgtaaa tccttcttct tttgtctttt
      961 gtgtcgcgtc gattccaagc tttgagccca agttcacaag gggcgaggcg ggatcaagcg
     1021 tatctgtcgg cgcattgttg ataattattg tgtcgcgtgc agggtcggca cgtgttgtaa
     1081 ttgcccaaat cacatcatcc atgctgtgca cgttgacgtc atcgtctact actacaaaca
     1141 tctttgttag ggcaagctgt cctagccccc agaggcccat catcaccttt tttgcctgac
     1201 cggggtatct ttttttaatt gaaattattg ccatgccctg gaaccaccca gcagcaggca
     1261 ttgcaaagtc gactacttct ggctggaaca ttttgatcag aggaagaaac gatctttcaa
     1321 ttactttgcc aatgtatgcg tcttctaata tcggcttgcc cactatggtg gtaaggtaga
     1381 ttggtttttt gcgcctcatg attccagtta acgtaaatgt cgggtatggc tcctttggcg
     1441 tgtagtatcc tgtgtggtct ccaaacggcc cctcgtctct gatgtctgat gggtcaacat
     1501 atccttctag gactagttct gcgtttgctg gaacctccaa atcaattgtc ctgcacttga
     1561 ctgtcttgat tcctttctgt ctggtaattc ctgcaaacaa gtacttgtct agtccctctg
     1621 gcactggagc aacagcagaa aatacggttg ctgggtctcc gccgatgatc actgctgcct
     1681 cgattttttt tccttgttct tttgagatat cgtggtggtg cgcgccgcgc ttgtgttttt
     1741 gccagtgcat gagcgcagtt ctctcatcaa gaatctgcat tcgataaacg ccaagatttc
     1801 tgacaccggt ctccgggtgc tttgttgcaa caaggccaag tgtgatgaat cgtcccgcat
     1861 ctttgtacca tgtctttaga attggaattt tgttaaatga cggggagtca tcgaagacct
     1921 cagttactgg cccactgcct tcaagcttgg ggaacacctc gcccatcttt gatagctctg
     1981 ggagtttttt tatcttgttc aaaatgccgg atgggatttc catttttgtc atatcgacaa
     2041 ttctttgccc gatttccgta aagtcggatg tctgaagacc gatttccagt cttttgagtg
     2101 agccaaacgc atttcccaag accggcatgt cgtagttttt tacattttca aaaagaatcg
     2161 cagggccgtt tgcatacatc gtccttctga gaatttcggc aatctccaaa tccgcatcga
     2221 cttgggtctt gactcgcctg agctcgcctg catcctctaa tgccttgata aattcggatg
     2281 tgtcttctat tggcacaact gcaggtttga gaatatgtgg tataaatctt gacgagaatg
     2341 cggtcttttc taaaatatca acaaaggcat tttaatcagc acgccaattt catactggtt
     2401 gagcgcagaa aaatgtgtgg tgtgcaaggg caagggtacg attgagcttt tggctacaca
     2461 atgcccgttt tgcaacggaa ctggggaata caacaaatct ggagacagct atcttaagtc
     2521 acacatctgc cagtgcatat ttttggacag aaagacgtgc ccgctttgcg gaaaaaaatg
     2581 tcatcacgat acaccaaaca agccaaaaat cttaattgga cccgtctgat cttttaggaa
     2641 tttactggat tggcggaagc tcgcttttct ttccgtggcc gcctgagccg aacttgcagt
     2701 aaaaacatag atccgactgc atgtgcgtca gcttttcaag ctggattgca ccaattttga
     2761 gtgctacttt tgttaggatc ttgtacttta gcggcatgga tggaattact tcgtccatgt
     2821 agactttgta gtgttctgga caaaacttga gtgtgacctt tgttggttct ttgcttgttt
     2881 ggtttacggt ttttgcaaaa ttgatctgct ctcgtatgta ctcgtgtttt ggacaaggac
     2941 agtcctttcc accaggatgt ttgtaggctg gtgtattttt ccagtcaaat tcagggaact
     3001 cgcgctcaaa gtcatccatt gggtagtatt gtttgttgct tctattaaat ttgatccaga
     3061 aatggatctg atattcatcc aaatgtaaat aaaccccgtt cattgagtta gacttagatg
     3121 tctagcggta agctaactaa aaaagacttg gaagccaaaa ttgcagaatt agaagcaaaa
     3181 cttgaaaaac tgtctgcaca aattgaagca aaaccagtac aaacaaaaac acctccacca
     3241 ccacaagtgc aaacaccacc aaaaccacaa acacaaacac ctccaccaaa accagcagaa
     3301 gaagc
//
LOCUS       DASWKH010000021         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1902182, whole genome shotgun sequence.
ACCESSION   DASWKH010000021 DASWKH010000000
VERSION     DASWKH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902182"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VGA92_01685"
     CDS             <1..981
                     /locus_tag="VGA92_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845157.1"
                     /translation="RPGVLGNYHGTDGLKISDIKDLEIQQNGDVCFTKIPAKVVVRSA
                     LSKAGHQYFTLLPKAGCQYLQGYLRERIADGEIIKPEAPVITFQKGYRTKTNGNTCHL
                     RTNTITKEIKGAFGIIIKERPYVLRSYFDTQLLLAESKGKITHAYRQFFMGHKGDIEA
                     KYTTNKNRLPESLTNDMREAYERSQNFLSPFEEEAVNEKNKKELFLEMWKEQAKLYGI
                     DPLKIRIEKQRIVQQQQNSTPDTSNATDIEIDAIKTAIQNTIRKNVKDATQPQTDGTK
                     YESKLVDNEDELVSCIQNGWEVVKELNSGKVLLRKTINTADRIQIPHHSN"
     gene            complement(904..1284)
                     /locus_tag="VGA92_01690"
     CDS             complement(904..1284)
                     /locus_tag="VGA92_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845158.1"
                     /translation="MKLESQTLKKELENLEQMAGIKTRYKIIWIPKTNSTKEGEVVGN
                     TIYIYSTNFTDALETLRHEFIDAMVSSVSKPYLDLVNALLYVISEKAYQKKEEIVESL
                     VGMMRDLYPVCCVDSFPKKDLATV"
     gene            complement(1353..2069)
                     /locus_tag="VGA92_01695"
     CDS             complement(1353..2069)
                     /locus_tag="VGA92_01695"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9845159.1"
                     /translation="MYGIYHKESTVKDYLIFKGGTSIRKIFFPGTWRFSEDLDFTVLP
                     DTPPEIITSGLDAIYDILENESGIKYEGEINVPHSGRAILGFIHFTGPIGMKNKVKID
                     ASRIEKMVDSPTSQTVTTSYGDLSNFQINGYTLNEVVAEKIRSLMQRSKVRDYYDIWK
                     MFSQANDFDTSLIGSMVQKKCTINNIEYSPSKIFDSSRMDGLREHWKKELERLVVEEL
                     PDPDVVFGNMKKLLAFLPKK"
BASE COUNT          735 a          477 c          430 g          646 t
ORIGIN      
        1 cgaccggggg tacttgggaa ctatcacggt accgacggcc tgaaaatctc tgacatcaag
       61 gatctggaga tccagcaaaa cggagacgtt tgttttacga aaattcctgc aaaagttgtg
      121 gtaaggtccg cgttgagcaa ggcaggccac cagtacttta cactcttgcc aaaagcagga
      181 tgccagtact tgcagggata ccttcgagaa agaatagcag atggcgagat cataaagcca
      241 gaagcccctg tgattacctt tcaaaagggc taccgaacaa agacaaacgg caacacatgt
      301 cacctgagaa caaacacaat aacaaaggaa atcaaaggcg catttgggat aatcataaag
      361 gagcgtccct acgttttacg gtcgtacttt gacacacagc tgcttttggc tgaaagcaag
      421 ggcaagataa cccatgccta ccgccagttc tttatgggcc acaagggcga cattgaggca
      481 aaatacacca ccaacaaaaa caggcttcct gagtcactga ccaacgacat gagagaggcc
      541 tatgagagaa gccagaactt tctctcgcct tttgaggaag aagcggtaaa cgagaaaaac
      601 aaaaaggagt tgttcttgga gatgtggaag gagcaggcaa agctctacgg aatcgaccca
      661 ttgaaaataa gaatcgaaaa acagcgaata gttcagcaac aacaaaactc cactccagat
      721 acatcgaatg ccactgatat cgaaatagat gcaatcaaga cagccattca aaacacgata
      781 agaaaaaatg tcaaagatgc gacacagcca caaacagacg gaacaaaata cgaaagcaag
      841 ctcgttgaca atgaagacga gctggtgtca tgcattcaga acggatggga ggtagtaaag
      901 gagctaaaca gtggcaaggt ccttcttagg aaaactatca acacagcaga caggatacaa
      961 atccctcatc attccaacta gcgactccac aatctcttct tttttttgat aagctttctc
     1021 agatattaca tacaggagag cattgactag atcaagataa ggttttgata cagaacttac
     1081 cattgcatca ataaactcgt ggcgtaaagt ttcaagagca tctgtgaaat ttgtagaata
     1141 gatgtaaatt gtatttccga ctacctcacc ttcttttgta gaatttgttt tgggaatcca
     1201 aattatctta taacgtgtct tgattccagc catttgttct aaattttcaa gttctttttt
     1261 gagtgtctgt gattctagtt tcatgtattc taaaaactga tttgcatata agacggcgtg
     1321 gcagacaagt ttcaagtaat cctaaacacg tattattttt taggaagaaa cgcaagcagt
     1381 tttttcatat tcccaaaaac tacatctgga tcaggtaatt cttcaacaac tagcctctct
     1441 aattcttttt tccagtgttc ccttagtcca tccatccgac ttgaatcaaa aatttttgat
     1501 gggctgtatt caatgttatt aattgtgcac ttcttctgaa ccatacttcc aataagcgac
     1561 gtatcaaaat cattggcctg actaaacatt ttccaaatat cataatagtc ccttactttg
     1621 cttcgctgca tcaagctgcg gattttttcg gctacaacct cattgagagt gtatccattt
     1681 atttgaaaat ttgaaaggtc cccataagag gtagttacag tttgtgaggt aggcgaatct
     1741 accatttttt ctattctgga ggcatcaatt tttaccttat ttttcattcc aattgggcct
     1801 gtaaaatgaa taaatcccaa aattgctctg ccactatgtg gaacatttat ttcgccctcg
     1861 tattttattc cactctcatt ttctaaaata tcataaatcg catcaagacc tgatgtaata
     1921 atttctggtg gagtatctgg caaaacagta aagtcaaggt cctctgaaaa tcgccatgtg
     1981 ccaggaaaaa agatttttct tattgatgtt ccacctttga aaattaaata atctttaact
     2041 gttgattcct tgtgataaat tccatacaaa agccattcta acgcataatc tttgtcaact
     2101 actcctgcag gttttccttg ctgccttgca agtacacgta tctgttcatc agtaagaata
     2161 ccaaatcacc tatgtcgtgg attttgcctc aaggatagat tctgttgaca cgtttacaaa
     2221 taatttccat ttcaaattgg attttttatt ttttgtagaa tgaggatcca accaggaacg
     2281 tcctgaac
//
LOCUS       DASWKH010000022        17257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_47188, whole genome shotgun sequence.
ACCESSION   DASWKH010000022 DASWKH010000000
VERSION     DASWKH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 17257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17257
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47188"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGA92_01700"
     CDS             complement(<1..291)
                     /locus_tag="VGA92_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845160.1"
                     /translation="MSNTIETKLAIFETFKTKHDELTGEALRQRAIIVSLATQNNPSQ
                     MTRTAIAQKIAQENGTVWKNIYSGIFRDFDEILIPLGIVEEAGRLPLRRGPRA"
     gene            406..1968
                     /locus_tag="VGA92_01705"
     CDS             406..1968
                     /locus_tag="VGA92_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX9845161.1"
                     /translation="MKNRNHKYALLLVIAVAATSTGVLSHAYAQQTTDGMDGYVLGTS
                     GIYTGNANECWYDNGEGGMLPCRIDTGDTAWILTATSLVLFMTPGVAFFYGGLARSKN
                     AVNTIGMVLVVMGLISVQWVLWGYTLAFGPNDNDANLYMGALDYVGFNKVSHYAPLGE
                     LGPCADTWSAAYQMNAMVDAEQCSVGWPGTVPHQMFAVFQLTFAIITPALIVGGLIDR
                     IKFSALVIFILLWATFVYDPLAHWVWGGGFIGGGSLDLNPDLSPSFGLDFAGGTVVHI
                     SSGFAALAGALVLGRRLGYGKVPMEPHNIPFVVLGASMLWFGWFGFNAGSEVMVDGIT
                     VSAWVVTNTATGMAVLTWLLMSWAHTGKPSIVGAATGAVAGLVAITPASGWVGPMASI
                     IIGIAAGTICYGCVAFKNSRKWDDALDVWGVHGMGGFTGAVLTGTLASPHIWDTGDGI
                     GAWTGTPEGYEQQAINIIGALMSVGYAFGVTIVILKIMDAVWPGGIRVTPKEEEIGLD
                     LAQHGERAYVNE"
     gene            2039..2812
                     /locus_tag="VGA92_01710"
     CDS             2039..2812
                     /locus_tag="VGA92_01710"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9845162.1"
                     /translation="MLITPKELAKELDNPNLILIDCRSFKEYSQGHVPGAVNLDLFGF
                     HWFDTSKEGLESFNEQSKRILSFVGITDEKKVVFYDDVSGMLAARGVWLLLYFSHKNV
                     LMLDGGIKKWQLEGLPLEQKTNGFTPSKFDGKINLDIITGYEHLNKNLNRLLILDARS
                     KEEYDGDVIRGARRGHIPGAVNIDWNLNISEDGAFKDEKSLSELYKFPKDTEIVTYCQ
                     GAYRAANSFLVLKKLGFQNVKVYLGSWGEWANRIDLPVE"
     gene            complement(2813..4906)
                     /locus_tag="VGA92_01715"
     CDS             complement(2813..4906)
                     /locus_tag="VGA92_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX9845163.1"
                     /translation="MVVAELKLGSIILPRSESPHAVSRLAEFEWFHKIETENDIVTPE
                     IDDLLLRAQKSYQSIDDVVKGLQIPLQVGMMEILFKGTVIKKKQYELDEIQTMIANVE
                     QKSPQIIDKPAKLLEEAAATRKSIDEYSTIQETFDVVKKLNVDVEGFGLMKHFYTNLF
                     VIDSDKFAEISRSLEGITIYRYELETKDKVAVIVIASATDADKVLKVFRAFNSNPFVI
                     PRELPQVPSQAYSLIESKLGELTQKQKKLQAEILTTKKSIRGDILSLHESAFLAKEVL
                     ETLRKPGGTKRFAVIQGYIPKNMEAKFKDVTKQWMSITEDIRDPKMLQNRPTLFNNKR
                     WIRTFEVITRSQGIPKRGEADPTPMIALMWPIFYGIMFADLGHGLLLMGFGLIFKLKG
                     QGTLSRWGMLIAISGASAAVAGVGVGEMFGFHIDHLEPFEKLLEGPLHPISWLVGVIT
                     VAELTFDQVITILKVSIFLGIIHLVWAFLLRIRKLHREGHKMIMFTEAIPNLTLYGGI
                     VVVMMCAIGSGYDVTNMYSRVHTEAVPWVTVFLGDWAQVWIITRISIIVVIASMVVMM
                     VGGILHTKHHPEEGGSVANVVMEVFLGKTVECLSHTISYARIGIMLLVHAALLLTVNN
                     SFASFGGFSSPAGIAMIVGGNIGIMMIEGLIVYIQSLRLHLYEFFTKWYDGGAQPFRQ
                     VVPELLYNQLIWKKK"
     gene            4964..5575
                     /locus_tag="VGA92_01720"
     CDS             4964..5575
                     /locus_tag="VGA92_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HEX9845164.1"
                     /translation="MPAAKIMGVDSALERTVEKILQSTEEQILASLQGSLKSSQETLS
                     NSRALLEQEYDKILSEGKKEAEKLEKQIIGNADLEARNKQLLLVEESIEKVFDAAIKK
                     IKDSDRNKDYTKLISLLLEESINTIGTSDIIIQTNSKDKPAVESSLSKFPGATLSSDP
                     IECLGGIKVQSKDGTMTFDNTIDARIERLKPLIRKNIASKFGR"
     gene            5581..7353
                     /locus_tag="VGA92_01725"
     CDS             5581..7353
                     /locus_tag="VGA92_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEX9845165.1"
                     /translation="MVAKGRIVWVSGPAVRADGMSDAKMYETVSVGDAKLIGEVIRLT
                     GDVAFIQVYESTSGLKPGEPVVGTGNPLSVLLGPGIIGQIYDGIQRPLKDLAQKSGSF
                     IGRGITTTPVDMSKKYNFKPLVKVGDEVKPGSVIGTVKETDLIDHSIMVPPDHPGGKI
                     KKIIGEGEYDLETELATTSDSKVPLKMYHYWPVRKPRPYKSRYDPTVPLLTGQRVIDT
                     FFPIAKGGTGSIPGAFGTGKTVTLHQIAKWADSQVVVYIGCGERGNEMTEVLVEFPHL
                     KDPRTGKPLMDRTVLVANTSNMPVAAREASIYTGVTIAEYYRDMGKDVVLVADSTSRW
                     AEALREMSGRLEEMPAEEGYPSYLASRLAEFYERAGRVRAQGSPDRDGSVTLIGAVSP
                     SGGDFTEPVTTHTMRFIKTFWALDAKLAYSRHYPSINWMNSYSGYLSDIGKWWSENVS
                     KDWFETRREAYGILQREDTLKEIVRLLGPEALPDEEKLILEVARMIKIGILQQNSFDE
                     VDTYCSPQKQFKLLTLLVEFYKKGQTALKEGASLADIRAMPIIATLLKARMEVKEDEL
                     AKLDQLMTTIDQEFKKITGVKVAA"
     gene            7350..8735
                     /locus_tag="VGA92_01730"
     CDS             7350..8735
                     /locus_tag="VGA92_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEX9845166.1"
                     /translation="MSTQGGVQYSKIAEIKGPLVIVDGVESVAFDELVEIQMTDGQKR
                     LGKVLEVGNRKAVVQVFEGTTGLSISGTSAKFAGKVMDMPVSEQVLGRVFDGLGRPID
                     GLPDPIAEKFIDINGAPMNPEQREYPRDFIQTGVSVIDGMLTLVRGQKLPIFSGSGMS
                     HNILAAQIARQAAVVGTTDEFAVVFAAIGVQFSEAEYFRRSLEESGALKRSVLFLNLA
                     DDPAIERIITPRVALTVAEYLAYDLGMHVLVILTDMTNYAEALREISAAREEVPGRKG
                     YPGYLYTDLSTIYERAGRLQGRKGSVTQMPILTMPSDDITHPIPDLTGYITEGQIVLG
                     RDLFRQGVYPPVNILMSLSRIMKDGIGEGRTRVDHQEVSNQNYDAYSRAQEVRALAGI
                     VGKAGLTGVDLKYLEVGDSFEKEFLSQGVDENRTIEETLSLQWKVVSLLPKNELTKVK
                     DKYIEQYYKER"
     gene            8740..9372
                     /locus_tag="VGA92_01735"
     CDS             8740..9372
                     /locus_tag="VGA92_01735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEX9845167.1"
                     /translation="MSFGQNVVATKIELLKYKRSSQVASMVQKILDDKRKVLLKNIEE
                     MITEANKARGGIWEPLQDVYKSVTDAYLSLGTATVDSVAQSTPAVMEVDAKVRRIVDV
                     SIPSLTVTEKDTKSMPYGFADTNSSIDRAAKQIKALLPKICKAAEYENSIFALAKALE
                     KTQKLLNALENVIIPQYRLRIKFILATLEEREREEFARLKKVKAVMEKKK"
     gene            9372..9518
                     /locus_tag="VGA92_01740"
     CDS             9372..9518
                     /locus_tag="VGA92_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845168.1"
                     /translation="MPKDDIKKLLDDANKSLGTEYENQVNSIKTELRSFKTKTLEKIQ
                     KTIP"
     gene            9553..9861
                     /locus_tag="VGA92_01745"
     CDS             9553..9861
                     /locus_tag="VGA92_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HEX9845169.1"
                     /translation="MRPFTLLLLATVVSLSAMTGLAFAQEEMTATSDSDSFKVLGAGL
                     AFGLAAGGAGVGLGYICSAGLAVISENPALQSKVFIFVGMVESIAIYGIVMMFIILGQ
                     "
     gene            complement(9876..10337)
                     /gene="pyrI"
                     /locus_tag="VGA92_01750"
     CDS             complement(9876..10337)
                     /gene="pyrI"
                     /locus_tag="VGA92_01750"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEX9845170.1"
                     /translation="MQESNLIVRRIKDGTVIDHIDGGKGLKVLEALGIDGKDGNVITI
                     ALNVPSGKFNKKDIIKVENRYLQDYDTNKLAVISPTATINIIKDYKLVEKRRVALPNK
                     IEKIFRCSNPECITNSQEHIESVMEVIDKSGRVLRCKYCTRILDVNQLKYY"
     gene            10379..11308
                     /gene="pyrB"
                     /locus_tag="VGA92_01755"
     CDS             10379..11308
                     /gene="pyrB"
                     /locus_tag="VGA92_01755"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX9845171.1"
                     /translation="MNDFYQKDIISVKDLDKTKFEKIFAATDKLIQMDPNQRRELGRG
                     NTLGYLFFEPSTRTRLSFQAAMALIGGTSIGIADVSSSSTKKGESLADTVRMMSIYSD
                     VLTLRHTLDGSSRFAAEISQKPVLNAGSGTEEHPTQAIQDLYTIKKEKNKIDGLKIGI
                     VGDLKYGRTVYSLLYALSNYDVDIRLISPKSLKIRTDSIYEIQQKLSLKESTNLEEFI
                     DELDVVYVTRIQKERFPDEEEYQKVRGSYNIGLDVVNKMKENSIILHPLPRIDEISTD
                     VDDTKQAKYFKQAEYGKFTRAALLALILNENGF"
     gene            complement(11310..12287)
                     /locus_tag="VGA92_01760"
     CDS             complement(11310..12287)
                     /locus_tag="VGA92_01760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9845172.1"
                     /translation="MSANKSLNRLRDHLEMMKRELGETTNQIKKLEDKNSTEWNSLDL
                     VEGKSIDDLKKHESELAQEISKIQKEIKKSHSKKLLVAELSVLPVVFLLIMFSGITDT
                     LTDSAPQQTMMKTRYYTENLRGDTVDTWKSWRLVGTTLNVNILKSPRVTEHQIDIIKN
                     AIASEKAVEFDDSIVHKGPKGSMSTYYEGWTGAIKSATQSQTKYNLPTSFHFIDTTGG
                     EGDIIITLSNIKDSDGYTGYTKSITEENEILKSFITIYDISNLSDQQLSTIIRHEFGH
                     ALGLGHSTAPEDLMAPSIDITIPYISECNVDAIVDLYNANEGKQTVCEK"
     gene            complement(12289..13089)
                     /locus_tag="VGA92_01765"
     CDS             complement(12289..13089)
                     /locus_tag="VGA92_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845173.1"
                     /translation="MTGEDMTLDVCSFEEVLPYSLMSIPISTVTTNDNVWIATSMLSR
                     ISDVTNNLVVIEDEFPIGTISVYEIIAGLQKNPTAQYFEQSVTKIMNADFYIDSRNTN
                     LANVLKRINRSKNPFTIIENDRTNFSQFSIRQVLEIGTLCKTNVGVSSFPKKQTPIFK
                     RDNTIRDVIGKLEQDENKLVLLEGGLSFVNHEILLEKIKELNTTQNDNLLELSASTFK
                     TITPTLISERLTISEICKIMLNMSYPCVMTSEQVITPHDILNLLCKEF"
     gene            complement(13306..15651)
                     /locus_tag="VGA92_01770"
     CDS             complement(13306..15651)
                     /locus_tag="VGA92_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845174.1"
                     /translation="MKVGIILAVLIVYALSASLAYAEEFDIRFLPSKLVENSQGRMHV
                     FISESGQIIPKKINDLTVTSLDSSILHIEKVEQSNAFTTEVIVKSGKPGTTTLYLAAP
                     GFTSKEIPVTIYGNKNNAATLLVKITPDTFTTSGHREGYISVELADEDGFPVIAKEDM
                     TISLSTANRDIVELSSSSMMIKKGEYFAYDKFQIKKSGEAVLYATAPGIQTQSSTVTV
                     EKDEDLTVKLYTYPETISIHDASRGFIIAQLQDSSGSPVLAQNDITVFYKVVDSDYSE
                     ATNYSTNYKQKSSGYFHIAKGSYWGYIEYSLPKGLEDTYDLSISTADPLTVEEVEIEA
                     EDLELMDDKLVKFESVPVLATGNRELIGIAYLEDENGNPVVAKNDMAVKIDSSDAKSL
                     SVEDAVFSRGNQMVLVYGKVGHSVLTSLELRPVVKEGERESVTVFGPNKDSLELVAEP
                     LISDVLVGTSFPMVLYLKDSDQVTSFLDDADVFLSPNEYVEIQKKKILQKDGLVVVDA
                     KSLKKGAVDLSVEVGDFKAEPTIDSLSSDPVSLVLDYSKTMFVGTNDVLSVQILNSGG
                     LPTYATNDVEINLVVKDQGILEAPAKVTIGKGSYYSLFDVAPKASGKTDVSLLSKELP
                     LLKKEITVASLALQLGISAPDSVDATETFIAKISAKVNEKPLVGVNVQWKVDGGIVQI
                     SDSKTGTTGEAIISIIPRAEIVNIEAVVSGEGYQASTVTKRVQINSNTAEVFVEEQQA
                     VSPEYEPFEVFGIDPVLIIVPGVIGAAGFMLKKKGQLTIKK"
     gene            15749..>17257
                     /locus_tag="VGA92_01775"
     CDS             15749..>17257
                     /locus_tag="VGA92_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9845175.1"
                     /translation="MGSQYEVVLNQIHLVNGLIKRLIENQDIKAELSPSEQPQLTFDN
                     KSKILSMLKVREKLQNRLPKQQNQIDKNLELLIGNFYYYDKQYEKATIFYDKSLKTDP
                     KNVSALINKGITTSKLGNHKEAITLYDKVLEIDPNYFDALHCKGDSLSLLSKHDEALS
                     CYEQTLTIDPTYFDGLYKKGLALFNLQNYEEATSYFDKALSIEPKHIPSLINKGITTS
                     KLGNHKEAIALYDKVLEVEPKYFDAIYNKGLALIALEEYEDALLCFDKTLEIDPHHAD
                     VLFNKGLCLAGLGKHQIAISYYDKALEFNAQHIVALYSKGCSFLQLGKKSDALGCFDK
                     AVEIDSNFEDAILYKGKVLLLLHDYKQAIKQFNTILNRNPANIVALYGKGVSLSGLGL
                     HEQAISYYDKTLEIFPNHFDAICKKGQILSLLGNNEKALECFERALKIKSGHHDSILN
                     KAFCLFRLERHQDALVFFDKALAINQGHTDTLVGKANSLSQLGRHQEALTFYD"
BASE COUNT         5371 a         3496 c         3476 g         4914 t
ORIGIN      
        1 tgccctaggc ccccttctca gtggcagtcg tcccgcctcc tcaacaattc caagtggaat
       61 gagtatctca tcaaagtcac gaaatatgcc agagtagata ttcttccaaa ccgtcccgtt
      121 ttcttgagca attttctgag caattgcagt tcttgtcatc tgtgaaggat tgttttgcgt
      181 tgctaaacta acaattatgg ccctctgacg cagtgcttca cctgttagct cgtcatgttt
      241 tgtcttaaac gtctcaaata tggcaagttt tgtttctatg gtattactca tagcataatt
      301 ttgcaatttt atgttattat cttaaaatga atctatataa caatttactt tttttgtgac
      361 tgaaacttcc ctatccttaa ataattttca cttttccaaa cctcaatgaa gaacaggaat
      421 cacaagtatg ctctattgct tgtgatagca gttgccgcaa catcaacggg agtattatct
      481 cacgcttatg cacagcaaac aaccgacgga atggatggtt atgtgctagg cacaagtggg
      541 atttacaccg gaaacgcaaa tgagtgttgg tatgacaacg gcgagggagg aatgcttcct
      601 tgtagaattg atactggcga taccgcatgg atacttacag caacatctct ggtcttattc
      661 atgaccccag gtgtcgcatt cttctatggc ggtttggcca gatcaaagaa cgctgtcaac
      721 actattggca tggtcttagt tgtaatgggt ctcatctcag tacaatgggt cctttggggc
      781 tacacgttag cctttggtcc aaatgacaat gacgcaaacc tctacatggg cgcactagac
      841 tatgttggat tcaacaaagt ctcccactat gcacctctag gtgagcttgg tccatgtgca
      901 gatacatggt cagcagcata tcaaatgaat gccatggtgg acgccgagca gtgtagtgta
      961 ggttggccag gtacagtacc acaccagatg ttcgcagtat tccaattgac attcgcaatc
     1021 atcacaccgg cactaatcgt tggtggactg attgacagaa tcaagttcag cgccttagtg
     1081 atcttcattc tcttatgggc aacctttgtc tatgacccac tagcacactg ggtctggggc
     1141 ggaggattca tcggaggagg atcattagac ctcaatccag atctgtcacc aagcttcgga
     1201 ctagactttg caggcggaac cgtagtccac atatcatccg gatttgcagc actcgcaggt
     1261 gcactagtcc taggaagaag actgggatac ggcaaggtcc caatggaacc acacaacata
     1321 ccgtttgtgg tcttgggagc aagcatgctc tggtttggtt ggttcggatt caacgcagga
     1381 agcgaagtga tggtcgatgg cattactgta agcgcatggg ttgtgacaaa caccgcaaca
     1441 ggcatggcag tactcacatg gctgcttatg tcatgggcac acacaggcaa accaagcatt
     1501 gtaggtgcag caacaggtgc agtagctgga ctagtagcaa ttacaccagc ctcaggttgg
     1561 gtaggaccta tggcatcaat cataatcgga attgcagcag gaactatatg ctatggatgc
     1621 gttgcattca agaattccag aaaatgggac gatgcactag acgtctgggg agtccacgga
     1681 atgggaggct ttacaggagc agtgctcact ggaacattag ctagcccaca catctgggac
     1741 acaggcgacg gaatcggcgc atggactgga acaccagaag gctacgagca acaagctatc
     1801 aacatcatag gtgcgttaat gtctgtagga tacgcatttg gcgtcactat tgtcatccta
     1861 aagataatgg atgcagtatg gccaggcgga atcagggtca ctccaaaaga agaagaaatt
     1921 ggacttgatc tagcacaaca cggcgaaaga gcatacgtaa acgaataaaa cccaaaacct
     1981 cttttctttt tgttttttta agaaaatgaa tttaatgttt attgaatcta gttgaactgt
     2041 gttaattaca ccaaaagagc ttgcaaaaga actggataac ccaaacctaa tactaattga
     2101 ttgccgctcc tttaaggaat actcacaagg acacgttcca ggcgctgtaa acttggattt
     2161 gtttggattt cactggtttg atacatcaaa ggaaggactc gaatccttta acgaacaaag
     2221 caaaagaatc ctatcgtttg tgggaatcac tgatgaaaaa aaagtggtat tttatgacga
     2281 tgtatcgggg atgcttgcag caaggggagt ttggttgctg ctatacttct cacacaaaaa
     2341 cgtcttgatg ctagatggtg gtataaaaaa atggcaatta gaaggattac cactggaaca
     2401 aaaaacaaat ggatttacac catcaaaatt tgacggaaag ataaaccttg acatcataac
     2461 aggatatgaa catctaaaca aaaatctgaa cagattgctg atattagatg cacgctcaaa
     2521 agaagaatat gatggggatg taattagggg agcacgtcgc ggtcacatac cgggtgcagt
     2581 caatattgat tggaacctca acatatcgga agatggggcc tttaaggacg aaaaatccct
     2641 ttccgaactg tacaagtttc caaaagacac agaaattgta acatactgtc agggtgcata
     2701 tagggctgca aactcgtttt tagtcctaaa aaaactcggc ttccaaaatg tcaaagtgta
     2761 tctcgggtct tggggagaat gggcaaacag aatagatcta ccggtggaat gattactttt
     2821 ttttccagat tagctgattg tacaataatt ctggcacaac ctgtctgaac ggctgtgctc
     2881 ccccatcgta ccactttgta aagaattcat agagatgaag cctgagggac tggatgtaaa
     2941 caattaatcc ttcaatcatc atgataccga tatttccacc aacaatcatt gcaattccgg
     3001 ctggtgatga aaagccacca aaagacgcaa atgagttgtt tacagtaagc aacaaggcag
     3061 catgaactag cagcatgatc ccaattcgcg catagctaat tgtgtgcgag agacactcta
     3121 ctgtttttcc taaaaacact tccattacca cgttagctac actacccccc tcctctggat
     3181 ggtgtttcgt atgcaaaatc ccaccaacca tcattactac cattgatgct atgactacga
     3241 ttattgaaat tcttgtaatt atccagactt gagcccagtc gcccaaaaat acggttaccc
     3301 atggaactgc ctcagtatga actcgagaat acatattagt aacatcatag cctgagccga
     3361 ttgcgcacat cataactact acaatcccac catacaaagt caagtttgga attgcctcgg
     3421 taaacattat catcttgtga ccttccctgt gtagtttcct gattcgaagt aggaatgccc
     3481 acacgaggtg gattattccc aaaaatattg aaaccttgag tattgtgatg acctgatcaa
     3541 aggttagctc agctacggta atcactccca ctaaccacga aattggatgg agtggacctt
     3601 caagtaattt ttcaaaaggt tctaggtgat ctatgtggaa tccaaacatc tcacctactc
     3661 caacacctgc gacagcagca gatgcaccag aaattgcaat taacatcccc caccttgaga
     3721 gggttccctg ccctttgagt ttgaagatta atccaaaccc cataagcaaa agaccatgcc
     3781 ccaaatctgc aaacattatt ccataaaata ttggccacat caaggcgatc attggagtcg
     3841 gatctgcctc accgcgcttt ggaattccct gactcctggt tatgacttca aaggttctaa
     3901 tccatctttt gttattaaac agagtcggtc tgttctgcaa catttttgga tctctaatgt
     3961 cttctgtaat cgacatccat tgttttgtaa cgtccttaaa cttggcttcc atgttttttg
     4021 gaatgtatcc ctgaattact gcaaatcttt ttgttccgcc aggtttgcga agtgtctcta
     4081 gtacttcttt tgcaagaaaa gcactctcat gtaaagacaa aatatcgcct cgaattgatt
     4141 tttttgttgt cagaatctcc gcttgtaatt tcttttgttt ttgtgtaagc tcgcctagtt
     4201 ttgactcgat taacgaatat gcctgcgatg gaacctgcgg cagctcacgt ggaattacaa
     4261 atgggttgga gttgaaagca cgaaagacct tcagaacttt atctgcatct gttgcactag
     4321 caatcacaat aactgcaact ttgtcctttg tctcaagctc gtagcggtat attgttatcc
     4381 cttctagaga acgacttatc tcagcaaatt tgtcagaatc aatcacaaaa agattggtgt
     4441 aaaaatgctt cattaatcca aagccctcaa catccacatt cagtttcttt actacatcaa
     4501 atgtttcctg tattgtggag tactcatcaa tggatttgcg cgttgcagct gcctcttcta
     4561 gtagtttggc aggtttgtcg ataatttgcg ggcttttttg ctctacatta gctatcatgg
     4621 tctggatctc gtcaagctcg tattgttttt tcttgataac agtacctttg aacaaaattt
     4681 ccatcattcc aacttgtagc ggaatctgaa gtcccttcac aacgtcatca attgactggt
     4741 aggacttttg agcacgcaag agcagatcat ctatttctgg tgttacaatg tcgttttctg
     4801 tctctatttt atgaaaccat tcaaattcag caaggcgaga aactgcatgt ggcgattctg
     4861 atctaggtaa aattatactc cctaatttta gctcagcaac aaccaagtca atttacggtt
     4921 tagaggacga ttttaaaatc tttccaaaca ttaaaatcca tttatgcctg cagccaaaat
     4981 aatgggtgtt gattctgcac ttgaacgcac tgtagaaaaa attcttcaaa gcacagaaga
     5041 acagatcttg gcaagcctac aaggatccct aaaatcatca caagagacac tttcaaactc
     5101 tagagccttg ctggaacaag aatatgacaa aatactttct gagggcaaaa aggaggcgga
     5161 aaagctagaa aaacaaatca taggtaatgc agatcttgag gctagaaata aacaactttt
     5221 gcttgttgaa gagtccattg aaaaggtctt tgatgctgct ataaaaaaaa tcaaagactc
     5281 tgatcgcaat aaagattaca ctaaattaat ttcattgttg ttagaagaat cgattaacac
     5341 cataggcact tctgatatta taattcaaac aaattcaaag gacaagccag ctgtcgagtc
     5401 ctcactttca aaatttccag gagcaacact ttcgtccgat ccaattgaat gtcttggcgg
     5461 aatcaaggtt caatcaaagg acggcactat gacatttgat aatacaattg atgcaaggat
     5521 tgaacgtctg aagcctttaa taaggaagaa tattgcatca aaattcggaa ggtagggtat
     5581 atggtagcaa agggtaggat tgtttgggtt agtggtcctg cagtaagagc agacggaatg
     5641 tctgatgcaa aaatgtatga gaccgtttct gtaggtgatg caaagctcat tggggaggta
     5701 attcgtctta cgggagacgt tgcatttatt caagtttacg aatctacaag tggattaaaa
     5761 ccaggtgagc ctgtagtcgg aacaggaaat ccgctaagtg ttttgcttgg acctggaatc
     5821 attggacaaa tttatgatgg aattcaaaga ccattaaagg atcttgcaca aaagtctggt
     5881 tcctttatcg gcagaggcat tacaacaacg ccagtcgata tgtcaaaaaa gtacaacttc
     5941 aagcctctcg tcaaagtagg cgatgaggtt aagcctggta gcgtaattgg cacagtaaag
     6001 gaaaccgatc taattgatca cagcataatg gttcccccag atcatcctgg tggaaaaata
     6061 aaaaagatta tcggtgaagg cgaatatgat cttgaaacag aattagctac taccagtgat
     6121 agcaaagttc cactcaagat gtatcattat tggcctgtta gaaagccgcg accatacaag
     6181 agcagatacg acccgaccgt tccgcttctg accggccagc gcgtcattga cacattcttt
     6241 ccaattgcaa agggaggaac aggttcaatt ccgggagcat ttggcacagg caagacagtc
     6301 actttgcacc aaattgcaaa gtgggcagat tctcaagttg tagtttacat tggatgtggt
     6361 gagcgaggaa acgaaatgac cgaagtactc gtcgagtttc cacacctaaa agacccaagg
     6421 actggtaagc cactcatgga cagaacggtt cttgttgcaa acacaagcaa catgccagtg
     6481 gctgcaaggg aggcaagcat ctacactggt gtaacaattg cagaatatta tcgcgacatg
     6541 ggcaaagacg tagttctagt agctgactca acaagcagat gggcagaggc cctaagagaa
     6601 atgagtggaa ggctagaaga gatgccagca gaagaaggat atccatcata cctcgcatca
     6661 agattggcag aattttacga aagagcagga cgtgtgagag cacaaggctc cccagatcgt
     6721 gatggctctg taactctaat tggcgcagtc tctccatctg gcggtgactt taccgaacca
     6781 gtaacaaccc acacgatgag attcatcaaa accttctggg cacttgatgc caaactagca
     6841 tattcaagac attatccatc aattaactgg atgaacagct attctggata tctatccgat
     6901 attggcaagt ggtggagcga aaacgttagc aaagactggt ttgaaacaag acgcgaggca
     6961 tacggaatat tacaaagaga ggacacacta aaagaaatcg taagactcct tggacctgaa
     7021 gcacttcctg atgaagaaaa actgattctt gaagtagcaa gaatgataaa aattggaatc
     7081 ttgcaacaaa actcttttga tgaggtcgac acgtattgca gcccgcaaaa acaattcaaa
     7141 ctcctgacat tacttgttga gttctacaaa aagggtcaaa ctgcgctaaa ggagggtgca
     7201 tcacttgcgg acattagagc aatgccaatt attgcgacac tactcaaagc aagaatggaa
     7261 gttaaagaag atgaattagc caaactagat cagctgatga ccacgataga tcaagaattc
     7321 aaaaagatta ctggagtaaa ggtggcagct tgagcacaca gggcggagtt cagtatagta
     7381 aaattgcaga aatcaaaggt ccactagtaa ttgtagacgg agtggaaagc gtagcatttg
     7441 atgagcttgt agaaatccaa atgacagacg gacaaaaaag actaggcaaa gtactagagg
     7501 ttggaaacag aaaagcagtt gttcaggtct ttgagggaac aacaggcctt tcaatttctg
     7561 ggacaagtgc caagtttgcc ggcaaagtca tggatatgcc agtatcagaa caagtgctgg
     7621 gacgagtctt cgatggtctt ggaaggccaa tcgatgggct tcctgatcca atcgcagaaa
     7681 aattcataga cattaacgga gcgccaatga atccagaaca aagagaatat ccacgagact
     7741 ttatccaaac aggcgtctcc gtcattgatg gaatgttaac acttgtccgg ggccaaaagc
     7801 ttccaatatt ttcaggatct ggaatgtcgc acaacatctt ggcagcacaa attgcaagac
     7861 aggctgcagt tgttggaaca actgacgagt ttgccgtagt ctttgcagca attggcgtac
     7921 agttctctga ggcagagtat ttcagaagaa gccttgagga atctggtgcg ctaaaacgaa
     7981 gtgtgttatt tttgaatctc gcagacgatc ctgcaattga aagaattatc acaccaagag
     8041 tcgcccttac cgtagctgaa tatcttgcat acgaccttgg tatgcacgtg ctagtaatct
     8101 taactgacat gacaaactat gcagaagcac taagagaaat tagtgcggca agagaagagg
     8161 ttccgggaag aaagggatat ccaggatatc tctatactga cctctcaaca atttatgaaa
     8221 gagcgggaag actacaaggt agaaaaggca gtgtgacaca gatgccaatt ttgacaatgc
     8281 catcagatga tatcactcac ccaattcctg accttactgg ttacattacg gaaggccaaa
     8341 tcgtccttgg acgtgacttg ttcagacagg gagtgtatcc gccagtaaac attctgatga
     8401 gtctgagcag aatcatgaaa gatggtatcg gcgagggtag gacaagagta gaccaccaag
     8461 aagtttccaa tcaaaactat gatgcgtatt ctagagcaca agaggtacgt gcactagcgg
     8521 gcattgttgg aaaggcaggt cttactgggg ttgacctcaa gtatctagag gtgggcgact
     8581 cgtttgaaaa agaatttttg tctcagggcg ttgatgaaaa caggacaatt gaggaaacac
     8641 tttcacttca atggaaggta gtatcacttt tgccaaaaaa cgagctaacc aaagttaaag
     8701 acaagtatat cgaacaatat tacaaggaaa gataactaga tgtcatttgg gcaaaatgtt
     8761 gtagcaacta aaatcgagct gctcaaatac aaacgctcaa gccaagttgc ttcaatggtt
     8821 caaaaaattc ttgacgacaa aagaaaagtt ttgttaaaaa acattgaaga aatgataacg
     8881 gaggcaaaca aagcgcgagg agggatctgg gaaccgcttc aagacgtcta caagtctgta
     8941 actgacgcat atctttcact tggaacagca actgtcgatt ctgttgccca atcaacacca
     9001 gccgtaatgg aagttgatgc caaagtaaga agaatagttg acgtatcaat tccatctcta
     9061 actgttacgg aaaaagatac aaaatcaatg ccttatggct ttgcagacac aaactcctca
     9121 atagacagag cggcaaaaca aatcaaggct cttttaccaa aaatttgcaa agcagctgaa
     9181 tacgaaaact ccatttttgc tttggcaaag gcacttgaaa aaactcaaaa actactaaac
     9241 gcccttgaaa atgtaatcat cccacaatac aggttgcgca taaaattcat cttggccaca
     9301 ttagaggagc gagaacgaga agaattcgca aggttaaaaa aagtgaaggc agtcatggag
     9361 aagaagaagt aatgccaaaa gatgatatca aaaaattact ggatgatgcc aataaatcgt
     9421 taggaacaga gtatgaaaac caagttaact cgatcaaaac cgagctgaga tcattcaaaa
     9481 ccaagactct ggaaaaaatt caaaaaacta taccatgatt taaataacgg aaatcgcgga
     9541 gcaaaatcaa aaatgagacc ttttacatta ttacttttgg ctactgtcgt atcactttca
     9601 gcaatgaccg gacttgcatt tgcacaagaa gaaatgactg caacatctga ttctgactca
     9661 ttcaaagttt tgggtgcagg cttggcattt ggtcttgcag caggtggtgc aggtgttgga
     9721 ctaggttaca tttgttctgc gggtctagca gtaattagcg aaaatccagc attacagtcc
     9781 aaagtgttca tattcgtagg tatggtcgag tcaatcgcaa tctacggtat agtcatgatg
     9841 ttcattatct tgggacaata agcccacaca tatttttagt aatatttcag ttggttcaca
     9901 tcaagaattc ttgtacaata tttgcaacgc agtactctac cactcttgtc aattacttcc
     9961 attactgact cgatgtgctc ctggctattt gtaatacatt ctggatttga gcagcgaaaa
    10021 attttctcaa ttttgtttgg aagggcaact cgacgttttt cgactagctt gtagtccttg
    10081 attatgttaa tggtagcagt tggagagatt acggctaact tgtttgtgtc atagtcttgc
    10141 agatatctat tttccacttt gatgatgtct tttttgttga atttaccgct tggcacgtta
    10201 agagcaattg taataacatt cccatccttt ccatctattc ctagcgcctc aagcactttg
    10261 aggccttttc cgccatctat gtggtcaatt acggttccat ccttgatgcg ccttacaatt
    10321 aggtttgact cttgcatcag aatactttgt atagtgacca ctatatatca ttggaagaat
    10381 gaacgacttt tatcaaaaag atatcatatc tgtcaaagat cttgacaaaa caaagtttga
    10441 gaaaatcttt gcggcaacag acaaattaat ccaaatggac ccaaaccaaa gacgcgagct
    10501 tgggcgagga aacaccttgg gatatctttt ttttgaacca agcactagaa cgcgacttag
    10561 ctttcaggct gctatggcat taattggagg tacctcaatt ggaatcgctg atgtttcatc
    10621 ttcgtcaaca aaaaagggcg aaagcctggc agatactgta cgaatgatgt cgatttactc
    10681 cgacgtcttg acattaagac acacacttga tggctcaagt aggtttgcag cagaaatttc
    10741 acaaaaacca gtccttaatg caggaagtgg aacagaggaa catccaaccc aagcaataca
    10801 ggatctttac acaatcaaaa aagaaaaaaa caaaatcgat ggactgaaaa taggcatagt
    10861 tggtgatctc aaatacggca gaacagtcta ttcattgttg tatgccctat caaattatga
    10921 tgttgatata cgactcatct ctccaaaatc tctcaaaatt agaaccgatt ctatctatga
    10981 aatacaacaa aaactatcac taaaagaatc aacaaatctt gaagagttca tcgatgagct
    11041 agatgtggta tatgtgacaa gaatacaaaa agaaagattt cctgacgaag aagaatacca
    11101 aaaggtcagg ggaagttaca acataggact tgatgttgta aataagatga aagaaaactc
    11161 cataatatta cacccactgc caagaattga tgaaatctca accgatgttg atgatacaaa
    11221 acaggcaaaa tacttcaagc aagcagaata tggcaaattc acacgtgctg cgttgcttgc
    11281 actgatacta aacgaaaacg ggttctaagc tatttttcgc aaacagtttg cttgccttca
    11341 tttgcattgt aaagatcaac aattgcatca acattacatt ctgaaatgta tgggatagtt
    11401 atgtcaattg acggcgccat taaatcctca ggtgcagtag agtgaccaag cccaagcgca
    11461 tgaccaaatt catgccttat gatagtagac aactgttgat ctgatagatt tgaaatgtca
    11521 taaattgtga taaatgattt tagaatttca ttttcttctg taattgattt tgtataacca
    11581 gtgtacccat cagaatcttt aatgttggat agtgttatga tgatatctcc ttctccacct
    11641 gtggtgtcaa tgaaatgaaa actggttgga aggttgtatt ttgtctgact ttgtgttgca
    11701 ctttttattg cacctgtcca tccttcataa taagttgaca tagaaccttt tggtccttta
    11761 tgtactattg agtcatcaaa ctctacagcc ttttcagatg ctatggcatt ttttataata
    11821 tcaatctgat gttcggttac ccgcggtgat ttcagaatgt tcacgtttag tgtagttcca
    11881 acaagtcgcc aagacttcca agtatcgacc gtatcacccc ttaggttttc agtatagtat
    11941 ctggttttca tcattgtttg ttgtggtgca gagtctgtga gagtatctgt tatgccagaa
    12001 aacatgatta gcaaaaacac aacaggtaga acagacagtt ctgcaacaag taattttttt
    12061 gagtgtgatt tttttatctc tttttgaatt ttagagattt cttgagcaag ttcgctttca
    12121 tgctttttta gatcatcaat tgattttcct tctaccagat caagcgaatt ccattctgta
    12181 ctgttcttat cttctagttt tttaatctga tttgttgttt cgccaagctc tcttttcatc
    12241 atctccaaat gatctcttaa tcggttcaat gatttattag ccgacaactt agaactcctt
    12301 acaaagaaga ttcagtatgt catgaggtgt gatcacctgt tcagatgtca taacacacgg
    12361 atagctcatg ttgagcatga tcttgcaaat ttcagaaatt gtcagtcttt ctgaaattaa
    12421 tgtcggcgta attgttttga aagtgctcgc acttagctct aagaggttat cgttctgggt
    12481 tgtgtttagc tctttaattt tttctaagag aatttcatga ttgacaaatg ataatcctcc
    12541 ttcaagtaaa acaagcttgt tttcatcctg ctccagcttt ccgatgacat ctctaatcgt
    12601 gttatctctt ttgaagatag gtgtctgttt ttttggaaat gaggatactc caacattagt
    12661 cttgcagagc gtgcctatct ccagaacttg tctaattgag aactgggaaa agttggttct
    12721 atcattttca attatggtga aaggattctt tgaccgattg attcgtttta ggacatttgc
    12781 gagattggta ttccgagagt caatataaaa atcggcattc attattttgg taacactttg
    12841 ttcaaaatat tgcgctgtgg ggtttttttg cagcccggca attatttcat aaacactaat
    12901 cgtacctatt ggaaactcgt cttcgattac caccaaatta ttggtaacat cggatattcg
    12961 tgacaacatg gaagttgcaa tccaaacgtt gtcatttgtg gttacagttg agattggaat
    13021 ggacattaac gaatatggga gcacttcttc aaaggagcaa acatcaagcg tcatgtcttc
    13081 tcccgtcaac aaagactgga ataccagagg ttttttttct ttagaagaac tcttgagaat
    13141 tcttccaagt ttaaagttta gattagctaa tttattctca actccttttt ccctatttta
    13201 gattctgaaa ctaaatttga taatcaagct gatgttcgaa ctgaacatac gatttctttg
    13261 attgtttaat aaacaaaaat tctgtttgat cttaatttac aaaatttatt tttttattgt
    13321 gagctgacct ttctttttta acataaagcc agctgcacct attactcctg gtacgatgat
    13381 cagcactgga tctatgccaa atacctcaaa tggctcatat tctggagaaa cagcttgttg
    13441 ttcttctaca aacacttcag cagtattaga attgatttgg actctttttg ttacggtaga
    13501 tgcctgatat ccttcaccac ttaccactgc ttcaatgttg actatctctg cccgtggaat
    13561 aattgaaatt atggcctcac cagttgtacc tgttttgcta tcggagattt gtacaattcc
    13621 gccatctact ttccactgca catttactcc gacaagcggt ttttcattta ctttagcaga
    13681 gatttttgct ataaatgtct cagtagcatc tactgaatct ggagcagaaa tgccaagctg
    13741 cagtgccaag cttgctacgg ttatctcttt tttgagtagt ggcaactcct ttgataacaa
    13801 tgatacatct gtctttccac tagcttttgg cgcaacatca aataggctat aataactacc
    13861 ttttcctatc gtcactttag ctggcgcttc tagaattccc tgatccttta ctaccaagtt
    13921 gatctcaaca tcgtttgttg catatgtagg taacccccca gaattgagaa tctgaaccga
    13981 aaggacatca ttagtgccaa caaacattgt ctttgaataa tctaagacaa gactgactgg
    14041 atctgaggac agactatcaa ttgtgggctc agccttgaaa tcaccaacct caacggaaag
    14101 atcgaccgct ccttttttta gcgatttagc atcaactact actagtccgt ctttttgtaa
    14161 aatctttttt ttctgaattt cgacatattc gtttggtgaa agaaagacat cagcatcatc
    14221 taggaaacta gtgacctgat cactgtcctt tagatacaaa accattggaa aactagtccc
    14281 tactagcaca tctgatatca atggctcagc tacaagttcc aacgagtctt tattaggacc
    14341 gaaaaccgta acactctcgc gctccccttc ttttacaaca ggtcttagct caagagaagt
    14401 taatacacta tgacctactt tgccataaac tagtaccatc tggtttcccc gtgagaaaac
    14461 tgcatcctcg acagaaagtg atttagcgtc ggatgagtca attttcacag ccatgtcatt
    14521 ttttgcaaca accggatttc cattttcatc ctccaagtac gctatgccaa ttaattccct
    14581 attaccagta gccaaaacag gcacactctc aaattttact agtttgtcat ccattaactc
    14641 caaatcttct gcctcaattt ctacttcttc aactgtaagt gggtctgctg tagaaatcga
    14701 aagatcatat gtgtcctcga gtcctttggg aagtgaatat tctatgtacc cccagtatga
    14761 acccttggct atatgaaaat agccagatga cttttgtttg taattggtac tgtagtttgt
    14821 ggcctcagaa taatctgaat ctacaacttt gtaaaagaca gtaatgtcat tttgcgctaa
    14881 taccgggctt ccactagagt cttgcagctg cgctatgata aaccctcttg aggcgtcatg
    14941 aatggagatt gtttctgggt atgtgtaaag tttgactgtg agatcctcat ctttttctac
    15001 ggttactgta ctgctttgag tttgaattcc aggtgcagtg gcatacaaca cagcttctcc
    15061 tgattttttg atctgaaatt tatcatatgc aaaatactcg ccttttttta tcatcatgct
    15121 tgagctagaa agctcaacga tgtctctgtt tgcagtgcta agtgaaatgg tcatgtcttc
    15181 tttagctatt acgggaaaac catcttcatc tgcaagctca actgaaatgt atccctctct
    15241 gtggccactc gttgtaaaag tatcgggagt tatttttact aaaagtgttg ccgcgttgtt
    15301 tttgttacca taaattgtaa ctgggatctc ttttgaggta aagccgggtg ctgcaagata
    15361 caaagttgtc gttccaggtt taccagactt tactattact tctgtagtaa aggcattact
    15421 ttgctcaact ttttcaatat gcagtatgga ggaatcaagc gatgtaacag taaggtcatt
    15481 gatctttttt ggaattattt gtccactctc tgaaataaaa acatgcattc ttccttgcga
    15541 gttttcaact agttttgatg ggagaaatct gatatcgaat tcctcagcat aggctaagct
    15601 agcactgaga gcatacacta taagtacagc taggattatt cctaccttca aacaacttgt
    15661 tgcactaaaa aaatcatata agacagtgtt tgagtttttt gaacaaatac tacgatatta
    15721 gaaaaaatct ttgaagtaaa gtaatgctat gggatcgcaa tatgaagttg tactaaatca
    15781 aattcaccta gtaaatggtc ttatcaagcg tttaattgaa aatcaggaca tcaaagctga
    15841 gcttagtcca agtgaacagc cgcagttaac gtttgataac aagtcaaaga tactcagtat
    15901 gctaaaggta cgagaaaaac tgcaaaaccg actaccaaaa caacaaaacc aaattgacaa
    15961 aaatctggaa ttgcttattg gaaatttcta ttactatgac aaacaatatg aaaaggcaac
    16021 cattttttat gacaaatcgc taaagacaga tccaaaaaat gtcagcgcat taatcaacaa
    16081 aggaatcacc acatcaaagc ttggtaacca caaagaagca attacactct acgacaaagt
    16141 actagaaatt gatcctaact attttgatgc cttgcactgc aaaggagatt cgctttcact
    16201 gctctcaaaa cacgatgagg cgctgtcgtg ttatgaacag acgctgacaa tcgatcccac
    16261 ttattttgat gggctttaca aaaaaggact ggcgctcttt aatcttcaaa attatgagga
    16321 agccacatca tattttgaca aggcactaag tatagaacca aaacacattc cgtcactaat
    16381 caacaaagga atcaccacat caaagcttgg caaccacaaa gaagcaattg cactttacga
    16441 caaagtatta gaagttgagc caaaatattt tgatgccatc tacaacaagg ggcttgcact
    16501 aattgccctt gaagaatatg aagacgcatt attgtgtttt gataagacgt tggaaataga
    16561 tcctcatcac gccgacgttc ttttcaacaa gggactgtgt cttgcgggcc ttgggaaaca
    16621 tcagatagca atttcttact acgataaggc actggaattt aatgcccaac acatagtggc
    16681 cttatacagc aaaggctgct cttttttgca gctaggaaaa aaatctgatg ccttgggatg
    16741 ctttgataag gcagtcgaaa tagattccaa ttttgaggat gcgatattat acaaaggaaa
    16801 ggtactgctt ttactacatg attataaaca agcaataaaa cagttcaata caattctgaa
    16861 tagaaacccc gccaacatag tggcacttta tggcaaagga gtctcattat ctggccttgg
    16921 cctgcatgaa caagctatat cctattatga caagacattg gaaatctttc caaatcattt
    16981 tgatgcaatt tgcaagaagg gccaaattct ttcattgctt ggtaataatg aaaaagcact
    17041 tgaatgcttt gagcgggcat taaaaatcaa atcaggacat cacgatagta ttcttaacaa
    17101 ggccttttgc ctattcaggc ttgaaagaca ccaagatgca cttgtcttct ttgacaaggc
    17161 gcttgcaatt aaccagggcc acacagacac actggtcggc aaggcaaact cactatcaca
    17221 gcttggccgc catcaagaag cactaacatt ctacgac
//
LOCUS       DASWKH010000023        15823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2271920, whole genome shotgun sequence.
ACCESSION   DASWKH010000023 DASWKH010000000
VERSION     DASWKH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271920"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VGA92_01780"
     CDS             complement(<1..580)
                     /locus_tag="VGA92_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX9845176.1"
                     /translation="MTTTRTIQVVFKLVPLIIALRKDRREWVRHEGKNVDYEKYQKNA
                     RKVLNTFVSLGPVYIKLGQWLSSRADILPQPYMEELAKLQDDVPAAPFDQVKPIIEGD
                     LGPISQAFTSIDTKPLSGASLGQVYRAKIQDKEVILKVKRPNIEKIIEEDIRVLKKVL
                     PLAMRFVDPNLRFSAHSMLAQFIETIHEELDYT"
     gene            complement(594..986)
                     /locus_tag="VGA92_01785"
     CDS             complement(594..986)
                     /locus_tag="VGA92_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845177.1"
                     /translation="MTWDEIEVPKELRPFMLEKAKETSLGHKNGASKQYRYGNLHIRE
                     YNDKFLVHMDKVDPLKNPLGHLLADAPEVLIGLASAAVGGTKIASYFYNKSNKTKNDK
                     QISTAVGLLSSLAIGYVGYRLAKKIKED"
     gene            complement(1027..2328)
                     /locus_tag="VGA92_01790"
     CDS             complement(1027..2328)
                     /locus_tag="VGA92_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845178.1"
                     /translation="MITRMVGIATLVFLGQFIGLQAYGQLGTGDSPFERNFGDIKFLD
                     AYFGRQDEKMEVTSGDKNVPFTVVFANVGSQDITGIKGQLQLPIGFGPADGKGALILA
                     DSDAEALAGKHFSLTFFVNLDKNIPIQQYPGTVKVDYSRLRESGQRNAFFDFKFKVTG
                     ESVLNLKAQNPFLTSLTNNDVTVEIANFGTAPLSNAKVVLQNTNTEVTATANPITNVE
                     SVVFDQNKWDIGTIDPKSSKKFTFRIYIPENVRSETLRTPMQITYFNAHGDRVIDNRI
                     VDFYINGLIDAKIYDVRVIDLSGQQTIIGEIINEGNINALFAFVTLEPLGDSNIKKTI
                     QFIDEVEIDSPVPFNIPVEFDGEPKYGEHEIRITLRYKDDLRSEHLLNHDTTVFLNDI
                     SKQPEPAISDYLLVIVGLAAASGVGAIIIKKIRKKENPIPS"
     gene            complement(2325..3035)
                     /locus_tag="VGA92_01795"
     CDS             complement(2325..3035)
                     /locus_tag="VGA92_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9845179.1"
                     /translation="MSDTVLEVKNLNKVYGRGDTEVHALMDVSFSVKRGEFLLIVGSS
                     GSGKSTLLNMIGLLDRPTSGQIYIDGVNTTKLTDNQISSFRNSKLGFIFQFSNLLADL
                     TVLENVLLPRNIQRTDKNAINDTINLLKAVGLESQMYKRANKISGGQAQRAAIARGLV
                     NKPAIVLADEPTGNLDSITAETTVQLMRSMAKKLNQTFIIVTHDRHQFGDADRIITIK
                     DGRAFEGEDQPHKMEITV"
     gene            complement(3028..4185)
                     /locus_tag="VGA92_01800"
     CDS             complement(3028..4185)
                     /locus_tag="VGA92_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9845180.1"
                     /translation="MLANKKGSLIGAVLAVSIGILVIHVNFVIFQGLYDAIVRDLSSY
                     RFGDVRVTDEEDFITKSDVFLVNWLERIPYVEAASSRIGSSVSINVTKNGKLIEEFQI
                     PVVGIDPFRDPRVSTVHETVKEGQYVFSRNSIVVGSSVARDLGGVRVGDSLQVQVTDR
                     FGQDQIRRFLVTGIATSPGGQGLDTSVIVHIDALRDMLSRNGETGEILVKLNDPKKAA
                     DVKDLFLRSFPNDDFRAETIEESAEEALSGFRSGIAMINMIGYFGMMSSAFAVVTIQM
                     MLVSSKTREVGIMRAIGAKRMDVLIIFIVQGMMIGAIGAGVGTAFGLTYTFYAKETKM
                     SFQGSLPLEVSYNWPNITQTALMAFALAVVASIYPAFKATKLQPVEAMRYV"
     gene            4332..5234
                     /locus_tag="VGA92_01805"
     CDS             4332..5234
                     /locus_tag="VGA92_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX9845181.1"
                     /translation="MDRLDLKILSVLLDNCRESDRQIGNRIGISGASVKSRIEKMIQN
                     GTIESFALKIEPPVLGYNVLYIVITGQDVDKILKQVELIGGPFFVVPCIGGITVCSIV
                     VKENVEQKIELVKKLMQNVRILSIFEAENPGIRSDLTKTDLEIINHLLKNPRIKIEEL
                     SRLTKLSTKTIARSLKKIQNDEAILFTVIYDPSKFGQYISFAVLVWVEENLNETLKQL
                     RKEFSSSFLQKPFLAKNQIVLFLYSNNIFELDNITQRIRDVKGVKTADLFIPKKINLP
                     QNWVSNIIKEAKSSQKLHLMYQRH"
     gene            5262..5777
                     /locus_tag="VGA92_01810"
     CDS             5262..5777
                     /locus_tag="VGA92_01810"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9845182.1"
                     /translation="MKKKTIYDGKILGLNIYDITVRGKKLKREIIEHRGAAAILAFDV
                     EGKVILVKQHRFGHGYILEIPAGTLEKGEKPEKCAYREIQEETGYKAKKLTHLITYYP
                     SVGYNKEAIYCFVAKNVQKISGQALDEDEIMSVVKISLKKLLNMIKMGKIIDSKTICA
                     VLAYALKNKIY"
     gene            complement(5771..6754)
                     /locus_tag="VGA92_01815"
     CDS             complement(5771..6754)
                     /locus_tag="VGA92_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEX9845183.1"
                     /translation="MPKFIRRLQKIGSSMLVSLPKEWVEKNHLEKSTEVELETNENTV
                     SITVNKGDRQSKEVVISYPLPQDENIVADITGAYLLGYDIIRVKGKTNIPIEDREKIR
                     NSMRRLVGMEIVEEDSSNVNVQFLLDATTLSPEKILKRISSIALGMFNDISSALISDD
                     KSNLQTFPNRDDEVDRQYFLLVRLIRSSMIDKRLATAFNLENIDVLDYRIAANILETA
                     SDTLVEIANSILHTSLSKNDLRRIYELTKDIENIHQKSTDAFIAQDRRLAIDAISSHR
                     KFQKKISDIRSSIEQKNQLPIDLLDLIYMFERVSRSWSDIADLVKPIYNAQ"
     gene            6819..7715
                     /locus_tag="VGA92_01820"
     CDS             6819..7715
                     /locus_tag="VGA92_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX9845184.1"
                     /translation="MKGVAFSPAHITGFFKAEMDQSQRPEFQGSLGAGFSIQAGVITT
                     VEIQDSDFIDYTIKVSGYQPDNTQVSEFVIKEFLKHVQGNYFVSVHHDIKIPVGYGLG
                     CSAAVALSLTYALNQAFNTNFSKERLGQIAHSAEVICQTGLGDVLASYHGGFEIRVKG
                     GAPGIGQVKKIPVESFSAIMICFSAISTKQFLKDKLQSINGLGGRMVDKLVNTKDIGQ
                     FHDYSVEFANYVNIITPKMKTVIDDLRAHGIKCGVALFGETIFTLVPQSLERDVLKIL
                     EKYNDGIIIRSKIDEHGARLVR"
     gene            7716..8471
                     /locus_tag="VGA92_01825"
     CDS             7716..8471
                     /locus_tag="VGA92_01825"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HEX9845185.1"
                     /translation="MTKLIIPNSHPRAKSLYIREKLVHAFDVGLVAKEGLMAHGRGEA
                     FDYLIGEKTSKTAKKAVKAAAFMLISAKKPVISVNGNIAGLCPREIVELAKTTGAKIE
                     INLFYESQKRKQNILKVLKKNGAKQVFGIEKKHLVKLSGLDSARRMVDKNGIYAADVV
                     LVPLEDGDRTLALKKAGKKVITFDLNPLSRTAQTADVTIVDNVIRGIREMINSCKIMK
                     RKKPSKIKFNNKKNLDQSIIEIRNHLTRRANHA"
     gene            8464..9303
                     /gene="panB"
                     /locus_tag="VGA92_01830"
     CDS             8464..9303
                     /gene="panB"
                     /locus_tag="VGA92_01830"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX9845186.1"
                     /translation="MHKSVKDIIDKKKSGNKISAITAYDYAMASLCDKAGIDILLVGD
                     SAGMVMLGYENTIPVTMDQMVLFTEAVSRARQNALVVADMPFMSYQASTSDAIANSGR
                     LIKAGADAIKLEGGRVVAPTIRAIVETGIPVMGHIGFQPQTTALAQGYRVQAKTKDAA
                     ITLIEDAKALEESGVFSIALEMVTSEVSKIISESVSVPTIGIGSGKYCDGQVLVVHDV
                     LGMYDKLKPKFVKQYLSLSDQITKAVTSYKLDVESGRFPSKENWFSMDKAELDKLREE
                     IGS"
     gene            9293..10531
                     /gene="coaBC"
                     /locus_tag="VGA92_01835"
     CDS             9293..10531
                     /gene="coaBC"
                     /locus_tag="VGA92_01835"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX9845187.1"
                     /translation="MVAKSKHPSFDITGSYGTELSGKKIVLCVAGSVAAYKAIELARL
                     LMRHGADVTCVASQAVTKMIQPDYFRWATGNKVITKLTGELEHIRLADYKRADLIIVY
                     PSTANTLGKLANGIDDTPISTVLTVGFGAKIPILMALAMHKAMYENAAVLRNIEFLKD
                     KIEFISPIMIEGKAKAAEPEDVLEHILRKFGFSTILAGKQVLITAGPTIEPIDPVRVI
                     TNQSTGKTGILLASELISAGAKVTLVYGPGITEPPKGVKIISVKTVNEMFDAVKKQLK
                     SKKFDIVILSAAAADYTVKANKSKIKSTEKHIVIKLQKAPKIIDQIKKIQKNVFLVGF
                     KAESNVSKKVLVDSAIRKLRDSSADMIVANDIGTKYQKNPELNEVVIVDGFGKTVSSG
                     RKTKLSISKFIRKQIELKLT"
     gene            complement(10573..12144)
                     /locus_tag="VGA92_01840"
     CDS             complement(10573..12144)
                     /locus_tag="VGA92_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9845188.1"
                     /translation="MIENENTWAKAVQSNATEEFHKKFDQALGSLKNELGKTYPLIIG
                     GKEVWTDNYFEVKSPADTKIVLAKFPLATKEETEYAIRAAKDAFSEWSQSHYQHRVGL
                     FREIASQLSSQKFTLAAIVSMENGKNRLEAMGEVDEMIDFLRFYADQLETNQGFVKPT
                     KSANPNEKTQSVLKPYGVWGIISPFNYPSAIAIGMSSGALITANTIVLKPASDTPISA
                     FKFVESIYRKIPAGAINLVTGQGNVVGQAIIDSPLVSGIAFTGSKEVGLSGFRTFTSK
                     NPRPFISEMGGKNPVIVTESADLEKATNGVLQAAFGYGGQKCSACSRVYVQKNIANKF
                     TEKLISKTQNLNIGLPWERNTYLGPVINEAAKKKFERAVELAKKDGKIIFGGEVLKDG
                     PYQNGYYVKPTIVTNLPKNHQLIKEELFLPFLCVQEFENYNDAIKEANDSEYGLTAGI
                     FSQNKKEIDEFFSKIEAGVTYANRAQSATTGAIVQSQPFVGWKNSGISGKGAGGVYYL
                     TQFLREQTQTICNES"
     gene            complement(12199..12330)
                     /locus_tag="VGA92_01845"
     CDS             complement(12199..12330)
                     /locus_tag="VGA92_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845189.1"
                     /translation="MKAKFATSCVSCGEKISPGKEIAKNEGGKWVHKHCAPESEQLL"
     gene            complement(12407..13201)
                     /locus_tag="VGA92_01850"
     CDS             complement(12407..13201)
                     /locus_tag="VGA92_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9845190.1"
                     /translation="MVIRNAKPSDKKPILDFCQRTFRWGDYIANVWDSWLSKGNLFAI
                     EAKGKPIGIFNTSFSKNQIWIEGLRINPKFRRKGYGSKLILKAEAIARQRGCKISRML
                     IAQHNTRSLLLAKSIGYKIEDKWWLYNLRPKKCTTKTRLATGVKQIEGLLDSNTYSKS
                     WEWFPLDKTTLARLVKKGRVITFSQNNKTSAIGIWNKSQIDRDVLQIGYLNGTKTGIN
                     AILHFMQNKAYIQKSKRIQILAQQKIDLKMKELNKRMLFCLMKKDL"
     gene            13490..14983
                     /locus_tag="VGA92_01855"
     CDS             13490..14983
                     /locus_tag="VGA92_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9845191.1"
                     /translation="MVIFVTIPAFGDEIWVIVETDKTQYSTGESLIVTGYIIERKMPV
                     IAMSVYDPDGIILSANSVDLEVDDSFTKTVSLDSPFYDKSGVYLIDFDYGKNTEQITF
                     EIVSSQFPQEQDHPTPEIIPEVIFLTTDKISYQNNEFITISGIVSIKNDPTILVGIYD
                     PSDMPAGFYTPEINSGLEFSVSFLAKDGVNFKTGGIYSVKAHYGTSKYVTNFEFVEVS
                     PPTNPISQQSDVQDEQDNDVVTQVPSTSIVNKPQIPLEKPSSKPAKTKIVDLKPSPGI
                     SGATDQPKQEEVDYNDLDNLTVEDVELGKILNEIMLNCDTNQYRDSIAHYDGMGPALM
                     RLCNYDQAISYFDQSLIEDPNSVEIITNKGAALDKLGQSDVAIDHYDLALDIDPTYLP
                     AMNNKANVLAEQGELEEAIKIYNLVLKQDPSYAISQTNLQKAKENLAAHMSSRQINLP
                     DTESSTSVEKLVKTETTVLERAQTSMPPSNIIQQIGSIFASFFGFLK"
     gene            complement(15255..>15823)
                     /locus_tag="VGA92_01860"
     CDS             complement(15255..>15823)
                     /locus_tag="VGA92_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9845192.1"
                     /translation="PPKIRCLLFVEFDSKIQQSIKKLKKTSTGKLLRTLNDKQSITKW
                     WNYRNSALYYSLKNLLPRESLPHIIEDATVPLDKLEQLITLAQKIRKKFHAKLVMYGH
                     AGNGNIHIRIASKYNDKTTTRRLGKMFFSEVIQMGGTITGEHGDGLARTDFVKLQYGP
                     KLYSIFNELKREFDPDLILNPNKIVTIR"
BASE COUNT         4806 a         2876 c         3035 g         5106 t
ORIGIN      
        1 tggtgtagtc tagctcttca tgtattgttt cgatgaattg tgctaacatc gagtgagcag
       61 aaaaacggag atttggatct acaaacctca ttgcaagtgg taggactttt tttagaactc
      121 taatgtcttc ttctattatc ttttcaatgt tgggacgctt gactttgaga attacctctt
      181 tgtcctgaat ttttgcgcga taaacctggc caagagatgc tccagatagt ggctttgtat
      241 caattgatgt aaaggcttga ctaattggac caagatctcc ttcgatgatt ggttttacct
      301 gatcaaaggg agctgcagga acatcgtctt gaagttttgc tagctcttcc atgtacggtt
      361 gcggtaaaat gtctgctctt gaggagagcc actgtccaag ctttatgtaa actgggccaa
      421 gcgaaacaaa tgtgtttaga actttgcgtg catttttttg atatttttca taatcaacat
      481 tttttccttc atgtcttacc cattctcttc tatcttttct tagtgcaata attaacggta
      541 caagtttgaa gacgacctga attgttcttg tggttgtgat gattattacc tccttagtct
      601 tctttgattt tttttgctag cctgtatcca acgtaaccaa ttgctaaaga tgacaaaagg
      661 ccaacggctg tagaaatttg tttgtcattt tttgttttgt tacttttgtt gtaaaaatac
      721 gatgctatct ttgttccacc cactgctgcg cttgccagcc caatgagcac ttctggcgcg
      781 tcagctaaaa gatgcccaag gggatttttg aggggatcaa ccttgtccat gtgaacaaga
      841 aacttgtcgt tgtattctcg aatgtgcaga tttccatatc ggtattgctt gcttgcgcca
      901 tttttgtgac caagtgaggt ctcctttgct ttctcaagca taaatggtct tagttccttt
      961 gggacttcga tttcatccca agtcatgatc ggtcattgct tttatctgat ataatcttta
     1021 gtgggtttag cttggaattg ggttttcttt ttttctaatt tttttgatta ttattgcgcc
     1081 tacgccagat gcagctgcca gaccaactat gactaaaaga tagtctgaga ttgctggttc
     1141 tggctgtttg cttatatcat taagaaaaac tgttgtatca tgatttagca ggtgttcact
     1201 tctaagatca tctttgtacc tgagagtaat tctgatctcg tgttctccgt attttggttc
     1261 accgtcaaat tctactggaa tattgaaggg taccggtgag tctatttcca cttcatcaat
     1321 aaactggatt gtctttttta tgttagaatc cccaagtggt tctaaagtta cgaatgcaaa
     1381 caaggcgttt atgtttccct cgttgattat ttcgccaatg attgtttgtt ggcctgaaag
     1441 atcaattacc ctaacatcgt aaatttttgc atcaattaga ccattaatgt aaaaatcaac
     1501 tattctatta tcgattaccc tatcaccgtg ggcgttaaag tatgttattt gcataggtgt
     1561 acgaagagtc tctgagcgaa cgttttctgg aatgtatatt ctaaatgtga atttctttga
     1621 ggatttagga tctattgtcc ctatatccca tttgttctga tcaaatacta cactttccac
     1681 atttgtaatt gggtttgcag tagcagttac ctcagtgttt gtattttgta atacaacttt
     1741 tgcatttgat aatggcgcag ttccaaagtt tgcaatttca acagttacgt cattatttgt
     1801 aagagatgtc agaaatggat tctgcgcttt cagatttaag acactttcac ccgttacctt
     1861 aaatttgaaa tcaaaaaatg catttctctg gcccgactct ctgagtcttg aatagtcaac
     1921 ctttacggtt cctgggtatt gttgaattgg gatgtttttg tccaaattca caaagaatgt
     1981 cagcgaaaaa tgttttcctg ccaaggcttc tgcatcgctg tctgctagaa tcagcgcacc
     2041 tttgccatct gcaggaccaa agcctatggg aagttggagc tgacccttta tgcctgtaat
     2101 gtcttgcgag ccaacgtttg caaacactac cgtaaatgga acatttttgt cgccagacgt
     2161 tacctccatt ttttcatcct gcctaccaaa ataggcatca aggaatttga tgtccccgaa
     2221 atttcgctca aaaggcgaat cacccgttcc aagctgccca tatgcctgca aaccaatgaa
     2281 ttgcccaaga aataccaatg tagcaattcc aaccatcctt gtaatcatac tgtaatctcc
     2341 attttgtgtg gttggtcttc tccttcaaat gctcgcccat ctttgattgt gatgatgcga
     2401 tctgcatcac caaactgatg acgatcgtgt gtcactatga tgaatgtctg attcagcttc
     2461 tttgccatcg atctcataag ttgaactgtc gtctcagctg ttatagaatc gagattcccc
     2521 gtaggttcgt ctgctaaaac aattgctggt ttgttaacta aacctcttgc aattgccgct
     2581 ctctgggctt gcccacctga gattttgttt gctcgtttgt acatttgact ttcaagtccc
     2641 acggctttta gcaaattgat cgtatcgttt atcgcatttt tgtctgttcg ctgaatgttt
     2701 cgcgggagca atacgttttc cagaactgtc agatctgcca acaagtttga gaattggaaa
     2761 ataaaaccaa gctttgaatt tctaaaagat gaaatttggt tgtcggttag cttcgttgtg
     2821 tttactccgt cgatatagat ttgaccactt gttggcctat caagcaaacc aatcatgttc
     2881 aataatgtgg atttcccaga tcctgagctt ccaacaatta gtaaaaactc gccacgctta
     2941 acagaaaatg agacatccat taatgcgtga acttctgtgt ccccccggcc gtagactttg
     3001 ttgaggtttt ttacctctaa gacagtatca gacatagcgc attgcctcca ctggttgaag
     3061 ttttgttgcc ttgaatgctg gataaattga ggcaacaact gcaagcgcaa atgccatcag
     3121 ggcagtctga gttatgtttg gccaattgta gcttacctca agtggaagac taccttggaa
     3181 ggacatttta gtttcttttg cataaaaagt ataagtcaaa ccaaaggctg ttcccactcc
     3241 tgcacctatt gcaccaatca tcattccttg gacgataaat attatcagga catccattct
     3301 ttttgcgcca attgcacgca taattcctac ctcacgagtc ttgcttgaaa caagcatcat
     3361 ttgaattgtt acaactgcaa atgcagaaga catcattcca aaatatccaa tcatgttaat
     3421 cattgcaatg ccagacctga atccgcttaa tgcctcctct gctgattctt ctattgtttc
     3481 agccctaaag tcatcatttg gaaatgaacg taaaaagaga tctttcacat cagctgcctt
     3541 ttttggatcg tttagcttaa ctagtatctc tcctgtttct ccgtttcgtg ataacatatc
     3601 acggagtgcg tcaatgtgta caattacact agtatcaagc ccttgaccac ctggtgaagt
     3661 ggcaatacca gtaacaagaa agcgtcggat ttgatcttgg ccaaatctgt cagttacttg
     3721 tacttgcagg ctgtcaccaa ctcttacacc gccaagatca cgtgcaacag atgatcctac
     3781 tacaatagaa tttcttgaaa aaacatattg cccttctttt actgtctcat gaactgttga
     3841 tactctagga tcacggaacg gatcaatgcc cacaacaggt atttgaaatt cttcaattag
     3901 ttttccattt ttggttacat tgatagatac agaagaccct atccttgatg atgctgcttc
     3961 cacgtatggt attctctcta accagtttac caaaaatacg tctgattttg tgataaagtc
     4021 ttcttcgtct gtcactctga catcaccaaa tctgtaactt gataggtcac gaactattgc
     4081 gtcgtatagt ccttggaata ttacaaaatt tacatgaatt accaaaattc caatcgaaac
     4141 tgccaaaact gcaccaatga ggctgccttt tttgttagcg agcattcgct tagctagctg
     4201 aatcctatag tccacgttat tataaaaaca gtctgatatt taatgtataa catgcatatt
     4261 atgatgattc aagacatttt tattaaaaaa gagtattctt taataagaaa taccattaaa
     4321 agaaggataa attggacaga ttagacctta aaattctaag tgttttgctt gacaactgta
     4381 gggaatcaga tagacagatt ggcaacagaa ttggcatttc tggcgcttcg gtaaaatcac
     4441 gcattgaaaa aatgattcaa aacggaacaa ttgagagttt tgcattaaaa attgagccac
     4501 ctgttttagg ttacaatgtt ttgtacattg ttataacagg gcaagatgtt gataagattc
     4561 taaaacaagt tgagttgatc ggagggcctt tttttgtggt gccatgtata gggggaataa
     4621 ctgtttgtag tattgtagta aaagagaatg ttgaacaaaa aattgaactg gtaaaaaaac
     4681 tgatgcagaa tgttcgaatt ctttctattt ttgaggcaga aaatcctggc atacgatcag
     4741 acctaacaaa gactgatctt gaaataatta atcatctttt aaaaaatcca cgtataaaaa
     4801 tcgaagaatt atcaaggcta acaaaacttt caacaaagac aattgcacga tcattgaaaa
     4861 agatacaaaa tgatgaagcg atattattta cagtaattta cgatccatca aaatttggcc
     4921 agtatatttc atttgctgtt ttagtttggg ttgaagaaaa tctaaatgaa acattaaaac
     4981 aattgaggaa ggaattttca agctcatttt tacaaaaacc gtttcttgca aaaaaccaga
     5041 ttgttttgtt tttgtatagc aataacatct ttgaattgga caacataaca cagaggatac
     5101 gagatgtaaa aggcgttaaa acagcagatc ttttcattcc aaaaaagatt aatttgccac
     5161 aaaattgggt aagcaacata atcaaggagg caaaatcttc acaaaaacta catttaatgt
     5221 atcaaagaca ttaaatatta tctaattaga aaaccaaacg aatgaaaaaa aagacaattt
     5281 atgatggtaa aattttaggt ttgaatattt atgacataac tgttcgcggt aaaaagttaa
     5341 aacgagagat aattgaacat cgtggcgctg cagcaatttt ggcctttgat gtggaaggta
     5401 aagtcatact tgttaagcaa caccgctttg gtcatggtta tattctggag attcccgcag
     5461 gcacgcttga gaaaggcgaa aaaccagaaa aatgtgccta tcgtgaaatc caagaagaaa
     5521 caggctacaa agccaaaaag ctaacccacc ttatcacata ctatccctct gtaggctaca
     5581 ataaagaagc aatttactgc tttgtggcca agaacgttca aaaaatcagt ggtcaggcct
     5641 tagacgagga tgagatcatg tccgtagtaa aaatcagcct caaaaagctt cttaatatga
     5701 taaaaatggg caaaataatt gattcaaaaa caatttgtgc tgttttggca tatgcgctaa
     5761 aaaacaaaat ttattgagca ttataaattg gttttactag atccgcaata tctgaccaag
     5821 atctcgatac acgttcaaac atatagatga gatctagcag atcaattgga agctggtttt
     5881 tttgctcaat tgatgagcga atatcagaga tctttttttg aaattttctg tgacttgaga
     5941 tggcatcaat tgctagcctt ctatcttgag caataaatgc atcagttgat ttttggtgta
     6001 tattttcaat atcttttgtc agctcataaa ttcttcgtag atcatttttt gaaagtgatg
     6061 tgtgcaaaat agaattagca atttcaacta gagtatcact tgctgtctct aaaatatttg
     6121 cagcaattct atagtctaga acgtcaatgt tttctaaatt aaatgcagta gctagtcttt
     6181 tgtcaatcat tgagcttcga ataagtctta ctagaagaaa gtattgtctg tctacctcat
     6241 catctctgtt tggaaatgtt tgcagatttg atttatcatc tgagatcaag gcagatgaaa
     6301 tatcgttaaa cattccaaga gcaattgagc ttattctttt tagaattttt tcagggctta
     6361 gtgttgttgc atccaaaaga aattggacat taacgtttga tgaatcctct tctacaattt
     6421 ccattccaac aagccttctc atcgaattgc gaattttttc tcgatcctca attggaatgt
     6481 ttgtttttcc tttaactcga attatgtcat agccaagaag atatgctccg gtgatgtctg
     6541 cgacaatgtt ttcatcctgt ggcagaggat acgaaattac gacttctttt gattgtcgat
     6601 cacccttgtt tacagtaatt gagacggtgt tttcattcgt ctcaagctca acttcagtac
     6661 ttttctcaag gtggtttttc tcaacccatt cctttggtaa tgaaactagc atgctgcttc
     6721 ctattttttg taggcgtcga atgaatttag gcaatttata ctaatttaat taatttaagc
     6781 catattctta tatttttgct ataatttaaa tttgaatcat gaagggagtt gcttttagtc
     6841 ctgcacacat tacgggattt tttaaggcag agatggacca aagccaaaga ccagaatttc
     6901 aaggttctct tggtgccggg ttttcgattc aggcaggtgt cataacaaca gttgaaattc
     6961 aagactccga ttttatcgac tataccatta aggttagtgg ataccagccg gataatacgc
     7021 aggtatcaga atttgtcata aaggaatttt taaaacatgt acaaggaaat tattttgttt
     7081 cggtccatca tgacataaaa attcctgtag gatatggact tggatgttct gctgccgttg
     7141 ctctttcttt gacatatgca ctaaatcaag catttaatac taatttttca aaggagcggc
     7201 ttggacaaat tgcacatagc gcagaggtga tatgccaaac aggacttggt gatgtgttag
     7261 catcatatca tggagggttt gaaatacgtg tcaagggcgg cgctccagga atagggcaag
     7321 tcaaaaagat tccagttgaa tcattttctg cgattatgat ctgtttttct gcaatttcta
     7381 caaagcagtt tctcaaagat aaattacaat caatcaatgg acttggtgga agaatggttg
     7441 ataaacttgt aaatacaaaa gatattggtc aattccatga ttattcagtc gagtttgcaa
     7501 attatgttaa tattataaca ccaaagatga agactgtgat tgatgatcta cgagcacatg
     7561 gaataaagtg tggcgtagcg ttgtttggcg aaaccatatt tacgctcgtt ccacagagtt
     7621 tggaaagaga cgttcttaaa attttagaaa aatataatga cggaattata attagatcaa
     7681 aaatagacga gcatggtgca agactcgtca gataaatgac aaaattaata attccgaatt
     7741 ctcatccgcg agcaaaatcg ctctacatca gagaaaagtt agtgcatgca tttgatgtcg
     7801 gtctagtagc aaaagagggg ctaatggcgc atggaagagg tgaagccttt gattatttga
     7861 ttggtgaaaa aacttccaaa actgcgaaaa aagcagtaaa agcagcggcg tttatgctta
     7921 tttcggcaaa aaaaccagtt atttcggtaa atggaaatat tgctggtttg tgcccaaggg
     7981 aaattgttga gttagcaaaa acaactgggg caaagattga gatcaatttg ttttatgaaa
     8041 gccaaaaaag aaaacaaaac attctcaaag ttctcaaaaa aaatggtgca aaacaggtat
     8101 ttgggataga aaaaaagcat ttggtaaaac tttctggtct tgacagtgcg agaagaatgg
     8161 tagacaaaaa tgggatttat gccgctgatg tggtattggt accactcgaa gatggtgata
     8221 gaacattagc actaaaaaaa gcaggaaaaa aagtaataac atttgatctg aatcctttat
     8281 cgagaactgc tcaaactgct gatgtcacca tagttgacaa tgtaattcgc ggcataagag
     8341 agatgattaa ttcatgtaaa attatgaagc gaaaaaaacc ttcaaaaata aaatttaata
     8401 ataaaaaaaa tctagatcag tcgatcatag agataaggaa tcatcttact aggagagcaa
     8461 accatgcata aatcagttaa agacattata gacaagaaaa aatctgggaa caagatttct
     8521 gcgattactg catatgacta cgccatggca tcattatgtg ataaagccgg aattgatatt
     8581 ttgcttgttg gtgatagcgc cgggatggta atgttaggat atgaaaacac gattccagtt
     8641 acaatggatc aaatggtctt gtttactgaa gccgtaagtc gagcaagaca aaatgcgtta
     8701 gttgttgctg acatgccatt catgtcttat caggcaagta caagtgatgc tattgcaaat
     8761 tcaggtcgat tgataaaagc tggagctgat gcaatcaagc ttgaaggagg cagggttgtt
     8821 gcaccgacca tacgagcaat agtggaaaca ggcattcctg ttatgggcca cattggtttt
     8881 cagcctcaaa ctactgcctt agcccaaggc tatagagttc aagcaaaaac aaaagatgct
     8941 gcaatcacgc taattgagga tgccaaagcg cttgaagaat caggtgtgtt tagtattgcg
     9001 ttggaaatgg ttacgtccga agtatcaaaa ataatttcag aatctgtttc tgttccaaca
     9061 attggtattg gttctggtaa atattgtgat ggacaggtgc tagtagttca tgatgtttta
     9121 ggaatgtatg acaagctcaa accaaaattt gtcaagcagt atctttcatt gtctgatcag
     9181 attacaaagg cggtaacaag ctacaaactt gatgtagaat ctggcagatt tccatcaaaa
     9241 gaaaattggt tttctatgga caaggctgag ctcgataagc taagagaaga aattggtagc
     9301 taaatcaaaa catccttctt ttgatattac tggatcgtac ggcactgagc tgtcaggtaa
     9361 aaaaattgtt ttgtgtgttg ctggaagtgt cgcagcatac aaggcaatcg agcttgcaag
     9421 gcttttgatg aggcatggag ctgatgtgac atgtgttgca agtcaagctg taacaaagat
     9481 gatacagcct gattatttta gatgggctac aggaaacaaa gtgattacca agcttacagg
     9541 agagcttgaa cacattcgtc ttgcagacta taaaagagct gatctaatca tagtatatcc
     9601 atcaactgca aacactctgg gcaagcttgc aaatggaatt gatgacacgc caatatctac
     9661 tgtgttaact gttggatttg gagcaaaaat tcctattttg atggcattag caatgcacaa
     9721 ggcaatgtat gaaaatgctg ctgttttgcg aaatattgaa ttcttgaagg ataaaatcga
     9781 gtttatttcc ccgattatga tagaggggaa agcaaaggct gctgagcctg aggatgtgtt
     9841 agagcacatt ttaagaaaat ttggattttc tactattctt gcaggcaagc aggtgctgat
     9901 cactgcaggc ccgacaatag aacccattga tcctgtgcgc gtgataacaa accaaagtac
     9961 aggtaaaaca ggaattttgc ttgcatctga gctgatttct gctggagcaa aggtaacttt
    10021 agtttatgga ccaggaataa ccgagccgcc aaagggagtc aaaataatat cagtgaaaac
    10081 ggtaaatgaa atgtttgatg ctgtcaaaaa acaattaaag tccaaaaaat tcgatattgt
    10141 gattttgtct gcagctgcag ctgattatac tgtaaaagca aacaagtcaa aaatcaaaag
    10201 cactgaaaaa cacattgtta taaaattaca aaaggcacca aaaattatcg atcaaataaa
    10261 aaagatccaa aagaatgtgt ttttggttgg attcaaagca gaatcaaacg tatctaagaa
    10321 agtactagtt gattctgcga taagaaagct cagagattca agtgctgata tgatagttgc
    10381 aaacgatatt ggtacaaaat atcaaaaaaa tccagagcta aacgaagtag tgattgtaga
    10441 tggatttggt aaaacagtaa gctccggacg caagactaag cttagcatct caaagtttat
    10501 cagaaagcaa atagagctaa aattaacttg atttatcctc tgaatttatt caagatatag
    10561 atttttcaga agctagcttt cattacagat tgtttgagtc tgctctcgca agaactgcgt
    10621 taggtaatac acgccgcctg ctccttttcc tgaaatacca gagtttttcc atccaacaaa
    10681 gggttgggat tgtacaattg cgcctgttgt tgcactttgt gctctatttg cgtatgttac
    10741 accagcctca atttttgaga aaaactcgtc aatttccttt ttgttctgac tgaatattcc
    10801 tgcggttaag ccatattcag aatcatttgc ttcttttatt gcgtcattat aattttcaaa
    10861 ttcctgaaca cataagaacg gcagaaacag ttcttctttg attagctggt gattttttgg
    10921 taaatttgtc acaattgttg gcttgacata atatccgttt tgatatgggc catctttgag
    10981 gacttctcca ccaaaaataa ttttgccatc cttttttgca agctcaactg ccctttcaaa
    11041 tttctttttt gcagcttcgt taatgactgg tcccagatag gtgttccttt cccatggaag
    11101 accaatattg aggttttgag tttttgatat caatttttcc gtgaatttat ttgcaatgtt
    11161 tttttgcaca tagactcgag aacatgcgct acatttttgc ccgccatacc caaatgcggc
    11221 ttgtaataca ccattagttg ccttttcaag atcagctgat tctgtaacaa ttactgggtt
    11281 ttttccaccc atttctgata tgaatggtcg cggattttta cttgtgaatg tcctaaatcc
    11341 cgatagccct acctcttttg atccagtaaa tgcaattcca ctaaccaaag gactgtcgat
    11401 tatagcttga cccaccacat ttccctgtcc tgttacaaga ttaattgcgc cggctgggat
    11461 ttttcgataa atagactcga caaacttgaa tgcggagatt ggagtgtcgc ttgctggttt
    11521 gagaactatg gtgtttgcag taattagtgc cccactgctc attccgattg caattgctga
    11581 tggataatta aatggagaaa ttattcccca aacgccgtac ggttttagaa cactttgagt
    11641 tttttcgttt gggtttgcac tcttggttgg ttttacaaat ccctggtttg tctcaagctg
    11701 atcagcataa aatctaagaa agtcaatcat ctcatcaact tcgcccattg cctcaagcct
    11761 attttttccg ttttccatac tcactatagc agccaaagtg aatttttgtg aagagagttg
    11821 acttgcaatc tctcgaaaaa ggccaacgcg atgctgataa tgactttggc tccactcgga
    11881 gaatgcatcc tttgctgccc tgatggcgta ctctgtttct tcttttgttg caagcggaaa
    11941 cttggctaag acaatttttg tatcagctgg agactttacc tcaaagtaat tgtcagtcca
    12001 gacctccttt cccccaatta tgagtgggta ggtcttgcca agctcgtttt tgagtgatcc
    12061 tagtgcctgg tcaaattttt tgtgaaactc ttctgttgcg ttgctttgta ctgcctttgc
    12121 ccaagtgttt tcgttttcaa tcaaggtatg tgatttagta aatcatctaa taaaatgatt
    12181 taggactggt taaaaaaatt acagaagctg ttctgattcg ggggcacagt gtttatgcac
    12241 ccattttcca ccctcgtttt tggctatttc tttgccaggt gagatctttt caccacatga
    12301 tacacatgag gttgcaaact ttgctttcat agtttggttt gatcttgatc tcttatcaac
    12361 aatatcattg atttattgtt gagctgaggt tagctaatga tcgattctac agatcctttt
    12421 tcataagaca aaaaagcatt cttttattca attctttcat tttaagatcg attttttgtt
    12481 gagcaagaat ttggattcgt tttgattttt gtatgtaggc tttattttgc ataaagtgca
    12541 agatggcatt tattcctgtt ttcgttccgt ttagatatcc tatctgcaag acatccctgt
    12601 ctatttgcga tttattccaa atgccaattg ctgatgtttt gttgttttga gaaaacgtaa
    12661 ttactctgcc ctttttgacc agtcttgcaa gcgtagtttt gtctagtgga aaccactccc
    12721 acgattttga atatgtgtta gagtcaagta agccttcaat ctgttttacg ccagtagcta
    12781 gacgtgtttt tgtggtacat ttttttggtc ttaggttgta taaccaccac ttgtcttcta
    12841 ttttatagcc aatggatttt gcaagcaata atgatcttgt attatgttga gcaatcagca
    12901 ttcttgatat tttgcatcct ctttgtcttg caatcgcctc tgcctttaag ataagcttac
    12961 tgccatatcc tttccttcta aattttggat ttatgcgcaa accttcaatc cagatttggt
    13021 tttttgaaaa cgaagtgttg aagattccaa ttggcttgcc ttttgcttca attgcaaata
    13081 ggtttccttt tgaaagccat gaatcccaaa catttgcaat gtaatcaccc catctaaagg
    13141 tcctctggca gaaatccaag attggctttt tgtcacttgg ttttgcgttt cgaattacca
    13201 acttgaattt tatgatatta atcgattatt tttcttgtta gctttatgtt actaatggaa
    13261 tatggagaag aggacaggct ttttgaactt taagtgtcta cggtaaatca agatctttga
    13321 gctggcattt agagtgttag ctgaactcat gactccacgt accttttgat ttactgggtt
    13381 atagattgct taaaaagtac aagtacaaat tatacgattt tattaaatac aaaaccacgc
    13441 atgcagacat gaattgggca tcacaagaca atggttgctc tctgcgatct tggttatttt
    13501 cgtcaccatt cccgcatttg gagatgaaat ctgggttata gtagagacag acaagacgca
    13561 atattctaca ggtgaatcat tgattgtcac aggctacatc atagaacgaa aaatgcctgt
    13621 aattgcaatg agtgtatatg atccagatgg catcatactt tcagcaaata gtgttgatct
    13681 cgaagtagac gatagtttca caaaaacggt ttctcttgat tcgccatttt atgacaaatc
    13741 gggagtttac ctaatagatt ttgattatgg gaaaaacact gaacagataa catttgaaat
    13801 cgtatcttct caatttccac aagaacaaga ccatcctacg cctgaaataa ttccagaagt
    13861 aatttttctt actacggata agatatcata tcagaacaat gaattcatta ctatttccgg
    13921 catagtttct ataaaaaatg acccgacgat tcttgttggc atatacgatc caagcgatat
    13981 gccagcaggg ttttacactc ctgaaatcaa ttcaggtttg gaattttccg tttcatttct
    14041 ggcaaaagat ggtgttaatt tcaaaacagg tggaatctat tcagtaaagg ctcattatgg
    14101 cacatcaaaa tatgtaacta attttgagtt tgttgaggtg tctccaccta cgaatcctat
    14161 ttcgcagcaa agtgacgttc aagatgaaca agataatgat gtggttactc aggttccatc
    14221 cacaagcatt gtaaacaaac cgcagatacc gttagaaaaa ccaagcagca aacctgccaa
    14281 aacaaaaata gtcgatttga agccaagccc gggaatatct ggtgccactg accagccaaa
    14341 acaagaagag gtggattaca atgatttaga caaccttaca gttgaagatg tcgagcttgg
    14401 aaagatatta aatgaaatca tgttgaactg cgatacaaat cagtatcgtg actccattgc
    14461 ccattatgat ggaatgggtc cggcattgat gagattatgt aattacgatc aagcaatttc
    14521 atattttgat caatcattaa ttgaagatcc aaacagtgtt gaaattatca ctaacaaagg
    14581 cgctgcatta gataagcttg ggcagtctga cgtagcaata gatcattatg atttggcact
    14641 tgacatagat ccaacatatc taccagctat gaacaacaaa gcaaacgtat tagcagaaca
    14701 aggagagctt gaagaggcaa ttaagattta caatttggta ttgaaacaag acccatcata
    14761 tgcgatatca cagactaatc tacaaaaagc aaaagagaat cttgctgcac atatgtcaag
    14821 tcgacagatc aatttaccag ataccgagtc ttccacgtca gttgaaaaat tagttaaaac
    14881 agaaacaacg gtgttggagc gtgcacaaac aagcatgcca ccatcaaaca taatacaaca
    14941 gataggtagc atctttgcaa gcttctttgg cttcctaaaa taagataata tgcacagtat
    15001 cgcgtatgat agcagtcttt ttaatacaaa aacatatttt ttgtacaaga gatcttgtgt
    15061 gttcatttca aaatcataat agattataga atatcaagca aatgaaaggt atggaagcta
    15121 aatgcaaatg ttgtggtaag aatttggagg atatgccgcc tgatgccaaa tattgttcca
    15181 tagaatgcac gtatggtatt ccagacttta aaaaagaggg tgaagtttac ccctaaatta
    15241 tttttaaata aactctatct tattgtcact attttgtttg gatttaggat tagatctgga
    15301 tcaaattctc tttttaattc attaaagata gagtagagtt ttggcccata ctgcaacttg
    15361 acaaaatctg tgcgtgcaag accatcacca tgttcacctg tgattgtccc acccatctga
    15421 attacttcgc taaaaaacat ctttccaagt cgtcttgttg tagttttgtc gttatatttt
    15481 gatgcaattc tgatgtgtat gttaccattt ccagcatggc catacattac aagttttgca
    15541 tgaaattttt ttctaatctt ttgtgccagc gtgataagct gttcaagctt atccaatggg
    15601 actgttgcat cttcgatgat atgaggaagt gactctcttg gaaggagatt tttgagacta
    15661 tagtacaggg ctgaatttct ataattccac cattttgtga ttgattgttt gtcgtttagc
    15721 gtacgcagta acttacctgt ggaggtcttc ttcagttttt tgatactttg ttgaattttt
    15781 gaatcaaact ctacaaataa aagacatctt atttttggtg gaa
//
LOCUS       DASWKH010000024        12956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1913618, whole genome shotgun sequence.
ACCESSION   DASWKH010000024 DASWKH010000000
VERSION     DASWKH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12956
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913618"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VGA92_01865"
     CDS             <1..1540
                     /locus_tag="VGA92_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9845193.1"
                     /translation="EVPVPKPVEVPVPKPVEVPVPKPVEKRVKKTTKKVRPDPVLIVL
                     SVAIFALLIYNVYSISTYLSLISIILAAAIAFYHILSRKPPQTQYQYKSRLASSYSMI
                     ILVLPFIFGTILAFEGYPTYVTLTQAIFVWALTLSFWQTMLFVPLAVRSAAREAMLEE
                     PNTWPKISVIVPAYNEENVIQTTIESLLSTDYPDKEIIVVDDGSVDKTLEIAMKYKDK
                     IKLIHKENGGKASALNQGFLYATGEIVTIVDADTIIGHSSLKHIAKSMVAENVAAVAG
                     NVKIRNKVNWLTWCQALEYLSGIQIMRRGLDYFGAITIVPGALGAFRKKKLEEAGTYH
                     KDTLVEDFDATMKVLRSGMIVSGSSAATAYTQAPQTLRDYYEQRKRWYRGNLQVLRRH
                     SDILLNPRFGYLQKLSYPLMAIHMLVIPIASVMLWVFVGYQLVIGNYQFVAFTLGIYI
                     TLQYLLSAMAVRMDNDDKRVILYSVFLVIGYKQLMDVLQIKAVIEEVLGQKAKWTSAK
                     RVRQ"
     gene            1541..4228
                     /locus_tag="VGA92_01870"
     CDS             1541..4228
                     /locus_tag="VGA92_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9845194.1"
                     /translation="MIDAKLAFVILAVCFVAIMPSALAQENTGAIDVFIKSENNDRLF
                     PQGVNIKVYKNQETTTTQEILSLENNPFSLSSLPLGHRYKVEVYVNDMYAGVGFVNLQ
                     KSKESLEITVKNMGGMRLNVFYKDAQTPLAGAYVVLKSQDGKSWHTSETDQYGNSIRA
                     WLYPSTKDGNYYYAEISLGPNLKYTYTPIKLQPDVAQEFKVVTSWPTIVDNLITVEVY
                     NSTKNKVTKQDGDFVAQLFDAKKSKVAESAVSDKGLAYFSNLKVNNYALYIKTKDSGN
                     QLQTVVGKSVTITDTTNTIKVYLNNPELNSDHLNCNCVAFRLDDIQDFYLAPAQLAII
                     SLFGQNDIPLTIGVIGGVIGTDQNLISTIKTSLVSDNPIEIASHSWNNKVLTTMTKKN
                     QEDLINQTNDKIKTVFGMQPTTFIPPENVFNDDTLSVLKNNGFTHISYDSREINPPQF
                     KKSDFHHFPVMPSTADLDTQTGIWHSSSNTQIMDSIDQSLLDYGYAVVMMHPYEFSVY
                     EGGFYVNKVNTTKVQELQSLIEKIQSKNLKILPIGSIQDYDNSLVEKPSNTKPEMPNC
                     NCVAFRLDNVQDFWLNDVQNTVLDTFNKKNAPLTISILGKFIGDDPKVVDHIKESLQN
                     KTTQIRLANRSWEYVDHTDYDLQKQLASIKQTNDKIFDVFGSRVVIFMPPYDAFNKDT
                     IEAVKQTKMIYFSASITNDPPPFPTDSLKHIPSTVSFANLSGDDPFLSGTTQQKALTK
                     VQASVKQHGFAVVSLHPPDFAVQTDVFENEVDADKMKLLESLIDDIRSSGLDVVTLEK
                     IPALLDEKSIVIPDWVKDNAAGWSEEKITDSDFTKGLEYLIDQNIIKIPSTQVEGDYT
                     QKIPGWIKINAAWWSEGKIGNYDFVKGIQYLIENGIIRI"
     gene            complement(4225..4542)
                     /locus_tag="VGA92_01875"
     CDS             complement(4225..4542)
                     /locus_tag="VGA92_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845195.1"
                     /translation="MSDTRIILLGVAFIFAGFLILGIFGQHHYEISVQAQEFGQCFEF
                     EDGIQTEIDCNVALQDRTVFFALVLGLIGVGIFFLIKGVRGRWDQDVKPQDKLGPDTS
                     FPS"
     gene            complement(4565..5041)
                     /locus_tag="VGA92_01880"
     CDS             complement(4565..5041)
                     /locus_tag="VGA92_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9845196.1"
                     /translation="MTYTIIHHSNNGHYLAKVSQAATLDALPKTIWDKISNIIGITEW
                     VIDVKKTEFLSKTRRGIGAIRKITFLDQSQVIEYVVGWKPENYLSYIATSGLPLDAYH
                     ATLDILPKGKMSQLTWTSFLISDSSDKKQFEEFLSFIDVFYEKSLQNLKAKIEKAT"
     gene            5126..6403
                     /locus_tag="VGA92_01885"
     CDS             5126..6403
                     /locus_tag="VGA92_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845197.1"
                     /translation="MVFGWGKKKDDAAEQISTQSQAISLSEIESILQEKKHKKQKLAV
                     QQTKPLFEKIQKEMGSIYKIISHLKSDDLKVDDIDKTLKVLVVRSKSEVIEIISKESQ
                     KTLPQISSYDDLLKASEISSHILKKIGDVLGKNSRVIHVFAKKYAQDLKTHLELITTH
                     QSTISKQIHVISSFDSSALTIKNIAEKILQFNNEIVEKTQHIDKLKEFQNECVDTINL
                     TEQKIVSMRSLPQFTKLLECQKQIKQVAVQQGGLNKEIDDEFSKISRALGKYVYVSSL
                     EKPFKVILEKLIANHADTISSENKDAIVTVLESCMKGIMSGTVSVKETDKSVAQITNL
                     ISLLDDFIARKTALQSQSKGLQEKLGIFDSRQLEELERQLDRAKLDKADTESKIKTLQ
                     ADLDNDYNQRQKLIEDLQSSLEGASGTKYTIQF"
     gene            6424..6807
                     /locus_tag="VGA92_01890"
     CDS             6424..6807
                     /locus_tag="VGA92_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX9845198.1"
                     /translation="MERKIILKKQARDGILTYCKIKHPNEGILILKGKSKNGIITVDG
                     LVIPPFSYSDQTFAGFPQSFLPLDLSYVGSAHSHPSGPAKPSITDLHNFFGLVSIIVK
                     SPYENNDDIFAWDSNGNQLEIIFDE"
     gene            6855..7181
                     /locus_tag="VGA92_01895"
     CDS             6855..7181
                     /locus_tag="VGA92_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845199.1"
                     /translation="MTVLFTYKNYLIFLFASLAVSIGLQMVLPFPYGLAAALAIFIAL
                     PLFIRRKYMSRMRGYGDSGSGGGGFFGFNSEGSGGVKYVCLTCNHRFKGAACPRCGSK
                     MKRADF"
     gene            7184..7507
                     /locus_tag="VGA92_01900"
     CDS             7184..7507
                     /locus_tag="VGA92_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845200.1"
                     /translation="MSLEKLRTQAIYQNSIDTWIAICEEKQLDWYETESYKKFIDYLF
                     KNGITMKKFPLCVKETGGMYERGKDKTKFAEMLAQLVDPNAAAYTIKLNDQTLKVIRD
                     FQITS"
     gene            complement(7504..8868)
                     /locus_tag="VGA92_01905"
     CDS             complement(7504..8868)
                     /locus_tag="VGA92_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9845201.1"
                     /translation="MSTFSSEIKQSFLKNKLGLAGIGILVLLILLSAFTFAFIPLDTY
                     KQWNNPASWTSFPRSAQPSWVNFFSEKKIPEHQIIDNPQTTLQQRGSTLTVLEKFETD
                     YEFDYFPNDFIFQYVAKYENSPLLQIDVIRPDGKTLRLVSLSLPESNGMSTYHDKIFS
                     TNDAIKKNLLLQSSEYSYQIDRIPSKEIIFSKSDSHEVLKGRYVFLVKLVGVDSQNLI
                     SESSLILGGKVFGLVGTDELRRDLSIGLMWGTPIALFIGIVVSIGSVVAGLVFGVYAG
                     YKGGKTDESMMRFNDIIYALPALPFLIILSVTTSNSIFLLIGFLMIFGWVGVAKVSRS
                     MALQIKTRQFVDAAKTMGQKDSKIIFRHIIPQILPYALASIAISVPAAITTEAGLSFL
                     GLGDPTFTTWGQILHDAKSYGAAARGLWWWILPPGIMIALTGLAFVFIGNSLETVANP
                     KLRR"
     gene            complement(8870..9922)
                     /locus_tag="VGA92_01910"
     CDS             complement(8870..9922)
                     /locus_tag="VGA92_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9845202.1"
                     /translation="MGLKRYFATRATILFGVLIATLFLTILLVGSNMDAIMKKGVAIQ
                     VRAEIVENKALASSFQSAGELDLYVQNQIEQRTKNLGLDTPWYSPNRIALSMYKILIL
                     DFGHATFLTSDGGSTAVSDIILEKLPRTILLFTTATIIISLIGIFVGAASGSNIHSKL
                     DKVTSSFAIISSSFPVWWVGLIMIFTFSFSYNLFPARATPLIAPTDPGYIGALLYHMT
                     LPLITIVLIGFGTWAYLVRNFMVGVMHEDFIFVKKAIGIKQSKIVFGSALKNAAPPIV
                     TILALSLSGSLGGAIITEAVFDWPGMGRLYFEAINVMDLPVIIGATYVLTAFFLVSIF
                     VSDLLYGYLDPRIRTI"
     gene            complement(9956..10321)
                     /locus_tag="VGA92_01915"
     CDS             complement(9956..10321)
                     /locus_tag="VGA92_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845203.1"
                     /translation="MRALVLEDNDRIIELYKKIFSQKKYEADFVSDTSSCLDRVTGQT
                     HGYDFVVLEKPTKLDNHANLEDQIRQASPKQKVFFLSPYMTQRDKGFEPVKATLDLVD
                     KPFALITLLSYLEIKTVQQ"
     gene            complement(10326..10751)
                     /locus_tag="VGA92_01920"
     CDS             complement(10326..10751)
                     /locus_tag="VGA92_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9845204.1"
                     /translation="MTSVQTQTVVLDSNVFIKEIQEGRVLRPIAKRMKRYKSKLVIPE
                     IVLGEISKITGADPIATMERVYQYCKQPITVDKTNEIVFESQRLAEKYYECHSPDNLI
                     LATAKVTGSTLVSFDRDLLQTAKMEGVQAFLPRNYIRWG"
     gene            10853..11695
                     /locus_tag="VGA92_01925"
     CDS             10853..11695
                     /locus_tag="VGA92_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845205.1"
                     /translation="MAENGEKSLAEKLDIAIENLSIWHRVTLVMVIIGLLSLSVVISL
                     SQYYFQQEISKHGEIEETKDEKTGQVGLEFVPHSDEVFSTESVLRLYVTQIWLMASNG
                     VVIGLLSFLFLWITFRGQAYKKDLKEIENQLIRQSYLVNFETSIPEGDTRVDKILSHS
                     ALVFPELQYLLRKAKPENKKLPYKLNQPIKNDTVDVLVSTKRGDFIVKFFDNVSFKTL
                     EEFTNNLSKSGKMIFRVLCVAKNFDEEMQSSQLSSLMQKLNLDFDIDLIFEEEQGYSM
                     LWIN"
     gene            complement(11692..12009)
                     /locus_tag="VGA92_01930"
     CDS             complement(11692..12009)
                     /locus_tag="VGA92_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845206.1"
                     /translation="MERKFNQTPGDTNIAILGYLTKVGTWMNLRQITTGTPGSDLNRE
                     RLRLILESFNKKGFAEIRDSQNPKAKFEYRITERGKNAFLKCVGFDPDVKYLFGVRDF
                     DAD"
     gene            12117..12362
                     /locus_tag="VGA92_01935"
     CDS             12117..12362
                     /locus_tag="VGA92_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845207.1"
                     /translation="MSASTIRKAKKLVEEGNIVKIADDLYQVRSSSDPNKSYVVTLDA
                     CDCLGFKNFYKFHHGKGIKANCSHLEAVRLYLKDQKG"
     gene            complement(12371..>12956)
                     /locus_tag="VGA92_01940"
     CDS             complement(12371..>12956)
                     /locus_tag="VGA92_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845208.1"
                     /translation="VVVTALEAQPETENPIVTNKPSYNTGQTVSVNGQVHSDFAGTPY
                     QILVYKNSDILVDTVDGKFGEDASYTQTIKTIDMAASEYTLKVVYGLPTQVGITTFKL
                     DSKQEPVIPTWIKNGAKWWASGEISDTEFIDAVEYLAKENIIVIQKTQPLEHIQSIPG
                     WLKTNASWWVDGLISDAEFVNGLEYLANVGIIQI"
BASE COUNT         4215 a         2588 c         2541 g         3612 t
ORIGIN      
        1 agaggtacca gtaccaaaac cagtagaggt accagtacca aaaccagtag aggtaccagt
       61 accaaaacca gtagagaaac gcgtaaaaaa gacaacaaaa aaagtcaggc cagatccagt
      121 gctcattgta ctgtctgtgg caatttttgc cttgttgatt tacaatgtgt atagcatctc
      181 tacatacctg agtctcattt cgattatctt agcagcggca atagcatttt atcacattct
      241 ctcaagaaag ccaccacaaa cacaatatca atacaaatct agattggcgt catcttattc
      301 tatgataatt ctagttttgc catttatctt tggtacaatt ttggcctttg agggttatcc
      361 tacgtatgtg actctgacac aagcaatatt tgtctgggcg ttgacactgt cattttggca
      421 aacgatgctt tttgttccat tggcagtgcg aagtgcagca agagaagcca tgctagaaga
      481 acctaacact tggccaaaga ttagtgtgat tgtgcccgcg tacaatgaag aaaatgtaat
      541 tcaaacaaca atagagtcac ttctttcgac tgattatcca gacaaagaaa taattgtagt
      601 tgatgacgga agcgtagaca aaacgcttga aattgcaatg aaatacaaag acaaaattaa
      661 actgatacat aaagaaaatg gaggtaaggc atcggctcta aatcaaggct ttctgtatgc
      721 tacaggcgaa atagttacca tcgtggacgc agatacaata attgggcact cctcactaaa
      781 acacattgca aaatcaatgg tggccgaaaa cgtagctgcc gttgctggca atgtcaaaat
      841 tcgtaacaag gtaaactggc ttacttggtg tcaggcacta gagtatctct ctggaatcca
      901 aattatgagg aggggcttgg attattttgg cgcaatcact atagttcctg gagcgttagg
      961 agcatttagg aaaaagaaac tagaagaagc aggtacatat cacaaggaca ctttggttga
     1021 ggattttgat gctacgatga aggtacttcg gtctggcatg atagtgagtg gaagcagtgc
     1081 cgcaacagcg tatacgcaag ctccacagac attacgtgat tattatgagc agagaaaaag
     1141 atggtaccgc ggaaacctcc aagtcctaag gagacactct gacattttgc taaatccgag
     1201 atttggctat ttgcaaaagc tttcataccc acttatggca attcacatgc ttgtaatacc
     1261 aattgcaagt gttatgcttt gggtttttgt aggataccaa ctagtaattg gcaactatca
     1321 atttgtggca tttaccttgg gcatatacat cacattacaa tacctgcttt cggcaatggc
     1381 agtacgaatg gacaatgacg ataaaagagt gatcctttat tcggtatttt tggtaattgg
     1441 gtacaaacaa ttaatggatg ttttgcagat caaggcagta attgaagagg ttttagggca
     1501 aaaagcaaaa tggacaagcg caaagagggt aagacagtaa atgattgatg caaaactagc
     1561 atttgtaatt cttgctgttt gttttgtggc aattatgcca tctgcacttg ctcaagaaaa
     1621 tactggtgca attgatgtat tcataaaaag tgaaaacaat gacaggctgt tcccacaggg
     1681 tgtaaatatc aaagtttaca aaaaccaaga aactaccaca actcaagaga ttctctcact
     1741 tgagaacaat cccttctcgc tttcatcttt gccgttggga cacagataca aagtcgaagt
     1801 atacgtaaat gatatgtatg ctggagtggg atttgttaat ctacaaaaaa gcaaagaaag
     1861 tcttgagatc acagtaaaaa atatgggtgg tatgaggcta aacgtctttt acaaagatgc
     1921 tcaaaccccg cttgccggtg cttacgtagt gctaaaatca caagatggaa aaagctggca
     1981 cacctcagag accgatcaat acggaaactc gataagggca tggctgtatc catctacaaa
     2041 agatggcaat tattattatg cagaaatctc acttggcccc aacctcaagt atacttacac
     2101 accaataaaa ctgcaacctg atgttgcaca agaattcaag gtcgtcacaa gctggcctac
     2161 aattgttgat aatctaatca cagttgaggt gtacaactct acaaaaaaca aagtcacaaa
     2221 acaggatggt gattttgtcg cacaactatt tgatgcaaaa aagagcaagg ttgcagaatc
     2281 tgcagtatcc gataaaggat tggcctattt ttcaaatctc aaagttaaca attatgcgtt
     2341 gtacattaaa acaaaggata gtggtaacca acttcagaca gttgtcggaa aaagtgtgac
     2401 aataacagat acaactaaca caatcaaagt ttacctgaac aatcctgaac taaatagtga
     2461 ccacctaaat tgtaactgtg tcgcgtttag gctcgatgat atacaggact tttatctcgc
     2521 acctgcccaa ttggcaataa tttcattatt tggacaaaat gatattccgc ttacaatagg
     2581 agtgataggg ggagtaatag gcactgacca aaacctaatt tccacaatca aaactagctt
     2641 ggtgtctgat aatccaatag agattgcaag ccacagctgg aacaacaagg ttttgacaac
     2701 aatgacaaaa aagaatcaag aagacctaat caatcaaaca aatgacaaaa taaagacagt
     2761 atttgggatg cagccaacaa cattcattcc accggagaac gtgtttaacg acgatactct
     2821 ctcagtgtta aaaaacaacg ggttcacaca cattagctat gattcaagag agataaatcc
     2881 accacaattc aaaaaatcag actttcacca tttcccagta atgccatcaa ctgcggatct
     2941 tgacacccaa acgggcatct ggcactcttc atcaaatact caaatcatgg atagcattga
     3001 tcaaagtctt cttgactatg ggtatgctgt tgttatgatg cacccgtacg aattttcagt
     3061 ctatgaaggt gggttttacg tcaacaaggt aaacacaacc aaggttcaag agttacaatc
     3121 attaattgaa aaaatacaat caaagaacct taaaatttta ccaattggct caatccaaga
     3181 ttatgataac tcattggtcg aaaaaccctc taatacaaaa ccggaaatgc ccaactgcaa
     3241 ctgtgttgca ttcaggctag ataacgttca ggacttttgg ctaaatgacg tgcaaaacac
     3301 ggttttggat acatttaaca aaaaaaacgc accactaaca atatcgatac ttggaaagtt
     3361 tattggtgat gatccaaagg ttgtcgacca cataaaggag agtctacaaa acaaaactac
     3421 ccagataagg cttgcaaata gaagttggga gtacgttgac catacagact atgatttaca
     3481 aaaacaacta gcaagcatca aacaaacaaa cgacaaaatc tttgacgtct ttggctcaag
     3541 agtggtaata tttatgcccc catacgatgc gttcaacaaa gacactatcg aggctgtaaa
     3601 acaaacgaaa atgatctact ttagtgcaag cataacaaat gatccacctc cctttcctac
     3661 tgactcgcta aagcatatcc ctagtactgt atcttttgct aatctgtctg gtgatgatcc
     3721 tttcttgtcg ggcactacac aacaaaaggc actgacaaaa gtacaggcaa gcgtaaagca
     3781 gcacggcttt gctgtagtca gccttcaccc gcccgacttt gcagtacaaa ctgacgtgtt
     3841 tgagaatgaa gttgatgccg acaagatgaa attgcttgaa tcactaatcg atgatattag
     3901 atcaagcgga ttggatgtag tgaccttaga aaaaatacct gcactattgg atgaaaaatc
     3961 aatcgttatt ccggattggg taaaagacaa tgcagctggg tggtctgaag aaaaaattac
     4021 tgattctgat tttacaaaag gactggaata tttaattgat caaaatatta taaaaattcc
     4081 aagcacacaa gtagaaggtg actatacaca aaaaataccc ggctggatca aaataaatgc
     4141 agcttggtgg tctgaaggaa aaattggcaa ttatgatttt gtaaaaggta ttcaatattt
     4201 gattgaaaat ggaataatca gaatctagct tggaaatgat gtgtctggac caagcttgtc
     4261 ttgtggcttt acgtcctgat cccacctgcc tctgactcct ttgattagaa aaaatattcc
     4321 tacacctatc agtcccaaaa ccagagcaaa aaatacggtt ctgtcctgca atgccacatt
     4381 gcagtctatc tccgtttgaa taccatcctc aaactcaaaa cattggccga attcttgggc
     4441 ttgcactgaa atctcgtaat gatgttggcc aaatatccct aaaatcaaaa aacctgcaaa
     4501 aataaatgca actccaagga gaattattct tgtatcactc atatctttga ttagctcaag
     4561 gtccttaagt tgctttttct atcttggctt tgaggttttg aagcgatttt tcataaaaca
     4621 catcaataaa cgacaaaaat tcttcaaatt gttttttatc gctactgtcg ctaatcaaaa
     4681 acgacgtcca tgttaactga gacattttgc cttttggcaa gatatctagt gtcgcatgat
     4741 aggcatcaag tggaaggcca cttgttgcaa tatatgacaa gtaattttct ggtttccaac
     4801 caaccacata ctcaattact tgactttgat ccaaaaaggt gatcttccta atggcaccaa
     4861 tcccacgtct agttttggac aaaaactcag tcttttttac atctattacc cattctgtta
     4921 tgcctatgat attgcttatt ttatcccaga tcgtctttgg caaggcatcg agtgttgcag
     4981 cctgagatac cttcgccaaa taatgaccgt tattgctgtg gtgtatgata gtatatgtca
     5041 tcccttgaag agtttttaat attttttaaa ttttttttaa caaaaattcg acagatatac
     5101 atttactaaa tactcaaagt taaacatggt ttttggatgg ggtaaaaaaa aggatgatgc
     5161 cgcagaacaa atatcaacac aatctcaggc gatatctctt tcagaaatag aatcaattct
     5221 tcaagaaaaa aaacacaaaa aacaaaaact agcagtacaa caaacaaagc ctctttttga
     5281 aaaaattcaa aaagaaatgg gatcaatata taagataatt tctcatctca aatcagacga
     5341 cttaaaagta gatgatatag acaagactct aaaagttcta gttgttcgta gcaagtctga
     5401 ggtaatagaa atcatctcca aagaatcaca aaaaacgctt ccacaaatct catcatacga
     5461 cgacttgttg aaggcttcgg agatctcttc acatatatta aaaaaaattg gagatgtgct
     5521 tggaaaaaac agccgggtta tccacgtgtt tgcaaaaaaa tatgctcaag acctaaagac
     5581 acatcttgag ctaatcacga cacaccaatc gacaatatca aaacagatac atgttatttc
     5641 ttcttttgac tcatccgcat taaccataaa aaatattgca gaaaaaattt tacaatttaa
     5701 caatgaaatt gttgaaaaaa cacaacacat tgacaaatta aaagaatttc aaaacgagtg
     5761 tgtagataca attaatttga cagaacaaaa aattgtctca atgcgttcct tgcctcagtt
     5821 tactaaactt ctagaatgtc aaaaacaaat aaaacaagtt gccgtccaac aaggtggtct
     5881 aaacaaggaa attgatgacg aattttccaa aatttcaagg gcacttggca agtacgttta
     5941 cgtatcatcg cttgaaaaac cattcaaggt aatactggag aaattgattg ctaatcatgc
     6001 tgatacaata tcatcagaaa acaaagatgc aatagtaacg gttttggaat cgtgcatgaa
     6061 agggatcatg tctggaacag tttcggtaaa ggaaactgac aagtcggttg cccaaataac
     6121 caatctgata tcactccttg atgactttat tgcaagaaaa accgccctgc aatcacagag
     6181 caagggcctg caagaaaaac ttggcatatt tgattctaga caacttgagg agctagaaag
     6241 gcagttagat agggcaaaat tagacaaagc cgatacagag tcaaaaataa aaacacttca
     6301 ggcagatcta gacaatgact acaaccaaag acaaaaactc atcgaagatc tgcagagctc
     6361 acttgaggga gcatctggaa caaagtacac aattcaattc tgaggtatgc attgaaatcc
     6421 aaagtggagc gtaaaattat actcaaaaaa caagccaggg atggcatact aacatactgc
     6481 aagataaagc atccaaacga gggaatcctc atactaaaag gcaaatcaaa aaacggcatc
     6541 atcacagttg acggactggt cattccgcca ttttcgtaca gcgaccaaac gtttgctggc
     6601 tttccacaat catttcttcc tctggatcta tcatatgttg gcagcgcaca ctcacacccc
     6661 agtggacctg caaaaccatc cattacagat ttgcacaact tttttggtct tgtctcaata
     6721 attgtaaaat cgccatatga aaacaatgat gatatctttg cttgggatag taatggaaac
     6781 cagctagaga taatcttcga cgagtagctg agatagaaaa aaactcacaa agctttataa
     6841 ggtttttgga aggtatgact gtgttgttta cttacaaaaa ctatctaata tttctatttg
     6901 catccttggc cgtaagtatt ggcctgcaga tggttttacc atttccatac ggtcttgcag
     6961 ccgcacttgc aattttcatt gctcttccac ttttcatccg ccgcaaatac atgagtagaa
     7021 tgcgaggtta tggcgactct ggcagtggtg gagggggatt ctttggattt aactctgaag
     7081 gcagtggtgg agtcaaatat gtatgtctta cctgtaacca tagattcaag ggagctgcct
     7141 gtcctaggtg tggctctaag atgaagcgtg ccgatttcta agtatgtcac tagaaaaact
     7201 aaggacgcag gcaatctatc aaaattctat agatacgtgg attgcgatat gtgaagaaaa
     7261 acaactagac tggtacgaga ctgaaagcta caaaaaattc attgattatc ttttcaaaaa
     7321 tggaataacc atgaaaaagt tcccactctg tgttaaggag actggaggaa tgtatgaacg
     7381 tggaaaggac aaaacaaaat ttgcggaaat gcttgcccag cttgttgatc ctaatgccgc
     7441 agcatacaca ataaaactaa acgaccaaac actaaaggta atacgggatt ttcagattac
     7501 gagttagcgc ctaagtttcg ggtttgcgac cgtttcaagt gaattgccaa tgaatacaaa
     7561 cgcaaggcct gtgagggcta tcattattcc gggtgggagt atccaccacc acagtcctct
     7621 tgcggcagca ccatatgatt tggcgtcgtg cagtatttga ccccatgtag taaatgttgg
     7681 atcccccaaa cccaaaaagc ttagccctgc ctcggtagtg attgcagctg gaacggaaat
     7741 ggcgatgctt gcaagcgcat acggcaaaat ctgaggtatg atgtgcctaa aaatgatctt
     7801 agagtctttt tggcccatag tctttgcagc atcaacaaac tgccttgttt tgatttgcag
     7861 tgccatgctt cttgagacct tggcaacacc cacccagcca aatatcatca aaaatccaat
     7921 caacaaaaat atgctgtttg aggttgtgac ggataaaatg atcagaaacg gaagggcggg
     7981 aagcgcataa atgatgtcgt tgaatcgcat catggactcg tcggttttgc cacccttgta
     8041 acctgcatac acgccaaaaa caaggccggc tactacagag ccaatagata ctactattcc
     8101 aatgaagagg gcaatggggg ttccccacat taatccaatc gataggtcgc gtctcaactc
     8161 gtctgtacca acaaggccaa agaccttgcc acccaaaatc agactagact ccgaaatcaa
     8221 attttgtgaa tcgactccaa cgagctttac caaaaatacg tatctgccct ttagcacttc
     8281 gtgactatct gattttgaaa aaatgatctc ctttgagggg attctgtcaa tttggtatga
     8341 gtattctgat gattgcagca aaaggttctt tttaatggcg tcatttgtag aaaaaatctt
     8401 gtcatggtat gtactcatac cgtttgactc tggaagcgac aaagacacca accgcaaagt
     8461 cttaccatct ggccttataa cgtcaatttg taggagtggc gagttttcat atttggcgac
     8521 atattggaat atgaagtcgt ttggaaagta gtcaaactca tagtctgttt caaatttttc
     8581 aagcactgtt agtgttgagc ctctttgctg aagtgtagtc tgaggattat caataatttg
     8641 atgttctgga atttttttct cactaaaaaa attcacccag ctgggctgag ctgatcttgg
     8701 gaatgatgtc cagcttgcgg gattgttcca ttgcttgtat gtgtctaatg gaatgaatgc
     8761 aaaagtaaac gcagacaaca atatgagcaa aactaggatt ccaattccag ccaatcctaa
     8821 tttgttttta agaaaactct gctttatttc ggatgaaaaa gtagacatct caaatagtcc
     8881 tgatcctcgg atctagatat ccgtacaaca aatccgacac aaaaatgctg actaggaaaa
     8941 atgcagtaag cacataggtt gccccaataa ttacgggcaa atccattaca tttattgcct
     9001 caaaatatag ccggcccatt ccgggccagt caaagacagc ttcagtaatt atcgcgccac
     9061 caagtgagcc ggagagactg agcgcaagaa ttgtaactat tggcggcgcg gcatttttca
     9121 gtgcactgcc aaagacaatc ttgctttgct ttatgccgat tgcttttttt acaaagataa
     9181 aatcttcatg catgactccg accataaagt ttctgacaag gtatgcccac gtcccaaaac
     9241 caatcaggac tattgtgatc aacggaagtg tcatgtggta aagcaatgct ccgatatatc
     9301 cgggatcagt tggcgcaatt aggggggtgg cccttgcagg aaataaattg tatgaaaaag
     9361 aaaatgtaaa gatcattatc aaaccaaccc accagacagg gaagctactg ctaattattg
     9421 caaatgatga cgttaccttg tctaacttgg agtgaatatt gcttccagac gcagcgccca
     9481 caaatatgcc aattagagaa attatgattg tagctgtagt aaaaagcaaa atggttcgtg
     9541 gaagtttttc caaaattatg tcacttactg cagtcgagcc accatcactt gtcaaaaatg
     9601 tcgcatggcc aaaatctaaa atgagtatct tgtacatgga aagggcgatt ctgtttggag
     9661 aataccatgg agtatcgagc cctaggttct tggttctctg ctctatttgg ttttgaacgt
     9721 acaagtcgag ctccccagca ctctgaaagc tgcttgcaag tgccttgttt tctactattt
     9781 ctgctctgac ttgaattgca acaccttttt tcattatggc gtccatgttg gaaccaacaa
     9841 gaagaatggt gagaaacaaa gtagctataa gaacaccaaa caagatggtt gctcgagtag
     9901 cgaaataccg cttgaggccc attgatcaga ttctattgaa atttgcttaa atcctctact
     9961 gttgcacggt tttgatctcc aaataggaaa gcaaggtgat gagggcaaaa ggcttgtcaa
    10021 ctaggtccaa ggtggccttg actggctcaa accccttgtc gcgctgggtc atgtatggtg
    10081 aaaggaaaaa gaccttttgt ttggggctgg cctgccttat ttggtcctca aggtttgcgt
    10141 ggttgtctag ctttgttggt ttttcaagaa ctacaaaatc atatccatga gtttggcccg
    10201 tgactctgtc aagacatgag gatgtgtctg agacaaaatc tgcctcgtat tttttttggg
    10261 aaaagatttt tttgtaaagt tcgataattc tgtcattgtc ctcaagaact agtgccctca
    10321 tcagtttagc cccaccggat ataatttctt ggaagaaatg cctggactcc ttccattttt
    10381 gcagtctgca acaaatccct atcaaagctt acaagtgtgg agccagtaac ctttgcagtc
    10441 gctaaaatca ggttgtctgg actgtggcat tcgtaatatt tttcagctag tctctgagac
    10501 tcgaagacta tctcatttgt cttatcgaca gttattggtt gcttacaata ttgatatacc
    10561 cgctccatcg tcgcaatcgg atcggcacct gtgatcttgc tgatctcgcc taggactatt
    10621 tccggaatca caagctttga cttgtatctt ttcatcctct ttgcaatggg cctcagaacg
    10681 cggccttctt gaatttcttt tatgaataca tttgaatcta aaactacggt ttgggtttgt
    10741 actgatgtca ttaacctaga ttgggtttag gtataaaagc aattttgcaa gaactgtatc
    10801 atgttacaaa ttgtaacaac tggttaaata cacacagaaa agctcaccat cgttggccga
    10861 aaacggcgaa aagtcgcttg cagaaaagct tgacatcgca atagaaaatc tcagcatttg
    10921 gcatcgcgtg acacttgtta tggtaattat tggtcttttg tcgctctccg tggtaatctc
    10981 gctctcacaa tattactttc agcaagaaat aagcaaacac ggtgagattg aagaaacaaa
    11041 ggatgaaaaa actggtcaag taggactgga gtttgtccca cactcagacg aagtcttttc
    11101 aacggaaagc gtccttaggt tatatgttac tcaaatttgg ctaatggcaa gtaacggtgt
    11161 agtcataggg cttctctcgt ttctgttctt atggattaca ttccgtggcc aggcgtacaa
    11221 aaaagatctt aaagaaattg aaaaccagct gataaggcaa tcatatcttg taaactttga
    11281 gacttcgatt ccggaaggag atacacgcgt ggataaaatc ctaagccact ctgctcttgt
    11341 atttccagaa ttgcaatacc tcctcagaaa agctaagcct gaaaacaaaa aacttccata
    11401 caagttaaac cagcctataa aaaatgacac agtcgatgta cttgtttcaa caaaacgagg
    11461 ggactttatc gtcaagtttt tcgataacgt ctcattcaaa acactagagg aattcacaaa
    11521 caacctctcc aagtctggca agatgatatt ccgagttttg tgtgttgcaa aaaactttga
    11581 tgaggagatg caatcatcac aactatcatc actaatgcag aagctaaacc ttgattttga
    11641 tatagacttg atctttgaag aagaacaagg atattcaatg ctatggatta actaatcagc
    11701 gtcaaaatct cttaccccaa aaagatactt tacgtcggga tcgaaaccga cacattttag
    11761 gaaagcattc ttgccccttt ctgtgatcct gtattcaaat tttgccttgg gattttggct
    11821 gtctctgatt tcggcaaatc cctttttgtt aaaactttcc aagatcagcc ttaaccgctc
    11881 cctgtttagg tcagagccgg gagtgcctgt agtaatctgt cttaggttca tccatgtgcc
    11941 tacctttgtc agatatccaa gaatagctat gttggtatct cctggggtct gattgaattt
    12001 tcgctccact tgttatccaa agaaaaactc aattgatatg ccttatgtgt tatatgaatt
    12061 aattatttgc actaaagatc gcaatctatt ctagtgacta aaacgaaata ttcctattga
    12121 gtgcaagcac gatcagaaaa gcaaaaaagc ttgtagagga gggcaacata gtcaaaatag
    12181 ccgacgatct atatcaggta agatcctcat ccgatccaaa caagtcatac gtggtgacat
    12241 tggatgcatg tgactgcctc ggcttcaaaa atttttacaa attccatcac ggaaagggaa
    12301 taaaggcaaa ctgctcgcac ctagaagcag ttaggttata cctcaaagat caaaaaggct
    12361 aacttgaaat tcagatctgt atgattccga cattggcaag atattcaaga ccatttacaa
    12421 attctgcatc tgagatcaaa ccgtcgaccc accacgatgc atttgtctta agccagcctg
    12481 gtatgctctg aatgtgttca aggggctggg tcttctgaat tacaatgatg ttttcttttg
    12541 caagatattc tactgcatca ataaattcag tatcagaaat ttcaccagat gcccaccact
    12601 tggcaccgtt ttttatccat gtcggaatta cgggttcttg ttttgagtca agtttgaagg
    12661 tggtaatgcc tacctgtgtg ggtagcccgt acacaacttt gagtgtgtat tctgatgcag
    12721 ccatgtctat agtctttatt gtttgtgtgt atgatgcatc ctcgccaaat tttccatcta
    12781 ctgtgtcaac tagtatgtca gaatttttgt aaactagaat ttgatatggt gtgcctgcaa
    12841 aatcactgtg aacttggcca tttacggaaa cagtctggcc agtattgtaa cttggcttgt
    12901 ttgtgacaat cgggttttca gtttctggct gggcttctag cgcagtaacg actaca
//
LOCUS       DASWKH010000025         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3379673, whole genome shotgun sequence.
ACCESSION   DASWKH010000025 DASWKH010000000
VERSION     DASWKH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3379673"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGA92_01945"
     CDS             <1..292
                     /locus_tag="VGA92_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9845209.1"
                     /translation="ALLLAGIDGIKQKLDPGDPIEENVYKLSSQQKRQYGIGSLPSSL
                     KEALDTLETDRKFLEHVFTKDFLDTYIELKYKEYTAFSQIPTAWEVSMYSDA"
     gene            complement(342..1748)
                     /locus_tag="VGA92_01950"
     CDS             complement(342..1748)
                     /locus_tag="VGA92_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9845210.1"
                     /translation="MLKSYVGEFNLTTLERGEFSNILSNIFPTNFQIGNADSNFFEAG
                     LARVLESLSSVSSDSIVSAPVLLLAAGVVIFLGVLGEAFFKRTGIPDIAFVMILGVIL
                     GPVLGLIQPEVVIEIVPYFAALALIIIMFDGGINLDIKSMARTAHFATVLSVFGFVIS
                     LAIVAILAHYGFGWDWLASVLLGSIVGGSSSVIVFGLVRNLHISEESKSLLSLESALT
                     DVLATIVAFVMFEAILIGSFELSTFSETMGRAVSVGLILGFGVGIPWMYVASKLSNSQ
                     HVYMLTLGILFVLYFMANAFGESGALTALVFGLMLGNRHHFARFLRFKLPKIETNDSM
                     HSQLTFLVRTFFFIFVGLLASFGNIEYMIFGAIAAIAIYMGRIILTKITITKRFSRLD
                     KKVISVMIPRGLAAAVLATFPITMGLPNAGAYPQIVFMIILVSVIITTIGLGSAKKIP
                     PPEPKGGGFIKDNKINDT"
     gene            complement(2055..3275)
                     /locus_tag="VGA92_01955"
     CDS             complement(2055..3275)
                     /locus_tag="VGA92_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845211.1"
                     /translation="MNKTLKYAGLLAILPLFTASLTTIAFENPSAEGQRVCGDKICTS
                     QNGPDAEKEREKMRQEIAGKTTTSETSQKEETKTFEESDLGSVLRLSRANVPATIPMH
                     EGFYNGEAVYFIITDSSDKTHADTITKNQGWKVELAPPLANAPKEALSKTYMFTNGVN
                     GNGVHGFQGEVFTSTPAQPEIYSALTSHVHVTWNEGTTPKVLDSEQAILDAQSVGEVS
                     LQDLKVVINMPQIVWPGGQMMVKEDKTLSDETPYGGGQVLDIDLDKMTVTFIAHRGWG
                     PDGRTIYYIVTDATPEMPANMMGVASAPTSAKLIANSAAVDLFQFMNGITGSGPMGFQ
                     AGIAVSAPGDQNYSPMWRISVIGWKDPTSAKLLETTTDISAFTKAGLIDVGLAGPMVG
                     GDHIVNCPFIDPFQ"
     gene            3386..4513
                     /locus_tag="VGA92_01960"
     CDS             3386..4513
                     /locus_tag="VGA92_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HEX9845212.1"
                     /translation="MNYQYPILGGIGVVAVLVIMYAAAVMTVPYQTEIDSIENTSIES
                     SMTSQEQSLSQIQITDGIKHIVPLDKIKSGGPPKDGIPSIDKPDFVTASEANFISDDD
                     LVIGLIINGKTKAYPLFILVWHEIVNDVVGGVPVAVTYCPLCFTNQVFERTINGQATE
                     FGTSGKLYNSNLVMYDRNTDSQWSQAIGTAITGELTGLTLKRVPFDVAKWSDWKTLYP
                     ETLVLTTKTGHIRAYGSDPYGDYYTDPQIIFPVENKDDRLHPKEIILGFDNGNIYKAY
                     KLADVEEKSVINDEIGDKKLLLVSLYPDMARAFNRVVDDTILEFQYIDGKIIDTQTNS
                     QWNLEGEAIKGQLIGKKLEREVFNPGFWFEWIAFHPNSLVY"
     gene            4522..>4953
                     /locus_tag="VGA92_01965"
     CDS             4522..>4953
                     /locus_tag="VGA92_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845213.1"
                     /translation="MKPLLKGIFVGVITVIVYLGVVVATTPALAPIDAISAALQLNSI
                     IIAGMGIGVGLQMYLSSYAKSFGCRLAIKRKTFSGNSGGTAFTSFLSFFSLVPLGCCG
                     WWLYALSFLPSIFGTGISAVLIEYSQPLSYLGLVIIFGFAGL"
BASE COUNT         1560 a          948 c          917 g         1528 t
ORIGIN      
        1 ggcattgctt ttagctggaa ttgatgggat aaaacaaaaa cttgatccag gagaccctat
       61 agaagaaaac gtctacaaac tttcttcaca gcaaaaaagg caatacggaa taggttcgct
      121 gccatcatca cttaaagaag cactggacac acttgaaaca gatagaaaat tcctagaaca
      181 tgtatttaca aaagattttc ttgacaccta cattgaacta aaatacaaag agtatactgc
      241 attttcgcag attcctactg catgggaagt ttcaatgtat tcagacgctt agcttttaac
      301 aaagttagag catgagatta tgctaaaaac atagaaaatc atcacgtgtc atttatttta
      361 ttatctttga taaaaccccc gccctttggc tctggaggtg gaattttttt tgcagaacca
      421 agacctattg tagtgattat tacagatacc aatattatca taaacacgat ttgtggatag
      481 gctcctgcat ttggtaagcc cattgtaatt ggaaatgttg ctagtacagc tgctgctaac
      541 ccacgtggta tcattactga gataactttc ttatcaagac gagaaaatcg ttttgttata
      601 gtaatttttg tgagaattat tcttcccatg tatattgcaa ttgctgctat ggcaccaaat
      661 atcatgtact ctatatttcc aaaacttgca agaaggccca caaaaataaa gaagaacgtt
      721 cttaccaaaa aggtaagttg actgtgcata gagtcattgg tttctatctt tggaagtttg
      781 aatcttagaa atcttgcaaa atgatgtctg tttccaagca ttagtccaaa aacaagagca
      841 gttaatgctc ctgactcacc aaaagcattt gccataaagt aaagaacaaa caagattcca
      901 agagtgagca tgtatacatg ttgactgttt gaaagctttg atgcaacata catccatggt
      961 attccaacac caaaacctag aatcaaccca actgagactg ctcttcccat agtttcagaa
     1021 aacgttgata attcaaaact gccaatcaaa atagcttcaa acattacaaa tgcgactata
     1081 gtagctagaa catcagttaa tgcagattct aaactaagca atgatttgga ttcttctgat
     1141 atgtgtaaat ttctaaccaa accaaatact atgacagagc tactaccgcc tacaatagat
     1201 ccaagtaaaa cactagccaa ccaatcccac ccaaatccat aatgtgcaag aatagcaact
     1261 attgcaaggg atatgacaaa cccaaaaact gacaatacag ttgcaaagtg tgcagtgcgt
     1321 gccatacttt ttatgtcaag attaatgcca ccatcaaaca tgattataat taaggcaaga
     1381 gctgcaaagt atggaactat ctcaatgacc acttcgggct gaattaatcc aagaactggt
     1441 ccaagaatta cacccaaaat cattacgaat gcgatatctg gaatacctgt tcttttgaaa
     1501 aatgcctctc ctaatacgcc caagaaaatt acaacaccag ctgctaggag taaaactggc
     1561 gcagaaacaa tagaatctga ggaaacagag cttagtgact ctagaactct agctaaccca
     1621 gcttcaaaga aattggaatc tgcattccca atctggaaat ttgttggaaa aatatttgat
     1681 aagatgtttg aaaattcacc tcgttccaaa gttgtaagat taaactctcc aacataagac
     1741 ttgagcattt caaaaatact atatagtaat atcagcatat atggccttat ttactttgaa
     1801 cttcactttg taaaattcga gaagatcaac attttttgat tttgtacatc taaacgtagt
     1861 ttgataaaaa aagctagtat gtaaaggaac tataaaattg gtttacctaa tccgacagca
     1921 acattcgtcc catgtacagt ttggacattc ttttggagaa ctgcttttct tacattcttc
     1981 aggcaaacaa ttacagtcag cacatcttat ctgcatattt ttcataattt ttcaaaaata
     2041 aaaatgagaa tccattactg gaatggatct ataaatggac agttaactat gtgatcgcca
     2101 ccaaccattg gtcctgcaag ccctacatca attaggcctg cttttgtgaa ggcactaata
     2161 tcagtcgtgg tttccaaaag ttttgcactt gttgggtctt tccagccaat cacagagatt
     2221 ctccacattg gactatagtt ttgatcacct ggtgcagata ccgcaattcc agcttggaat
     2281 cccataggtc ctgatcctgt gattccgttc ataaactgga atagatcaac tgctgccgag
     2341 tttgcaatta gttttgcaga agttggtgct gaagcgactc ccatcatatt tgcaggcatc
     2401 tcaggtgttg cgtctgtaac aatgtagtag attgttctac catctggacc ccatccccgg
     2461 tgagcaatga atgtcacagt cattttatca agatcaatgt caagtacctg acctccacca
     2521 tatggtgtct catcagaaag tgtcttgtcc tcttttacca tcatctggcc accaggccaa
     2581 acaatttgtg gcatgttgat tacaactttg agatcttgca gcgaaacttc acctacagat
     2641 tgtgcgtcaa gaattgcttg ttcagagtca agcactttag gcgttgtacc ctcgttccat
     2701 gttacatgaa catgtgatgt tagtgcgcta tagatctctg gctgagctgg tgtgcttgta
     2761 aaaacctcac cttgaaatcc atggacaccg tttccgttta caccattagt aaacatgtat
     2821 gtttttgaca gtgcttcttt tggtgcattt gcaagtggtg gtgcaagttc gactttccat
     2881 ccctgattct ttgttattgt atcagcatgt gtcttgtcac ttgaatctgt gatgataaaa
     2941 tacactgctt caccattgta gaatccctcg tgcattggaa ttgtggcagg aacatttgct
     3001 cttgaaagtc gtagtacaga accaagatca ctttcctcaa atgttttagt ttcttccttt
     3061 tgggatgttt ccgatgtagt tgtttttcca gctatctctt gtcgcatttt ttcgcgttct
     3121 ttttcagcat caggaccatt ttgtgaagta caaattttat ctccacaaac tctttgtcct
     3181 tcagctgaag ggttttcaaa agctatggtg gttaatgaag ccgtaaatag cggtagaatt
     3241 gctaacaatc ctgcgtattt tagtgtctta ttcattgcat gctcgttttt ctaatcaatt
     3301 aaaagcatta ttccaaatta gatccaaaag tgcaatagca tttggaacaa ttttttaata
     3361 ctttcttaac cgtttttttt gcaaaatgaa ttatcaatat ccaattttag gaggcatagg
     3421 cgtagtcgct gttttagtaa taatgtatgc agctgcagtg atgactgtac catatcaaac
     3481 ggaaattgac tctattgaaa atacaagtat tgaatcatct atgacatctc aggaacaaag
     3541 tttatcgcaa attcagatca cagacggaat caaacacatt gttccactcg ataagatcaa
     3601 aagtggcggg ccgccaaaag atggaattcc atcaattgac aagccagact ttgtaactgc
     3661 gtctgaagcc aattttattt ctgatgatga tcttgttata ggtcttataa taaatgggaa
     3721 aacaaaggct tatccacttt tcattcttgt ttggcatgag atagtaaatg atgtggtagg
     3781 tggcgtgcct gttgcagtca catactgtcc tctatgtttt acaaatcagg tttttgaaag
     3841 aacaatcaac ggacaagcta ctgaatttgg aacctctggc aaactataca acagcaattt
     3901 agtgatgtat gacagaaata ctgattcaca gtggagccaa gcaattggaa ctgctataac
     3961 tggcgaacta acaggcctaa cactaaaaag agtcccattt gatgttgcca agtggtctga
     4021 ctggaagaca ctgtatccag aaacacttgt tttgacaaca aagacaggtc acataagggc
     4081 ctatggctct gatccttatg gtgattacta tacagatccg caaattattt tcccagttga
     4141 aaacaaagat gataggcttc atccaaagga gataatttta ggatttgata acggcaatat
     4201 ctacaaggca tacaaacttg ccgatgtaga agaaaaaagt gtgataaacg acgaaattgg
     4261 tgacaaaaaa ttacttttgg tctctctata cccagacatg gcaagggcat ttaacagagt
     4321 tgtagacgat acaattttgg aatttcagta cattgatggt aagataattg acacgcaaac
     4381 aaacagtcag tggaatcttg aaggagaagc aatcaaaggt caactaattg gtaaaaaact
     4441 tgaaagagaa gtcttcaatc ccggattttg gtttgaatgg attgcatttc atccaaactc
     4501 acttgtttat tgagatgtga aatgaaacct ctgctgaagg gaatttttgt aggagtaata
     4561 acagtgattg tgtaccttgg cgtagttgta gctactactc ctgcacttgc tccaattgat
     4621 gctatttctg ctgcactgca attgaattct ataatcattg caggtatggg aataggagtt
     4681 gggctccaga tgtatctttc aagctatgct aaaagttttg gatgcagact agcaattaaa
     4741 agaaaaactt ttagtggaaa ttcgggtggt actgcattta catcattttt atcatttttc
     4801 tcactagttc ctcttggttg ctgcggttgg tggctatacg cactttcatt tcttccaagt
     4861 atttttggta ctgggatttc tgcagttctg attgagtata gtcagccttt atcatatttg
     4921 gggttagtta ttatctttgg atttgcagga ttg
//
LOCUS       DASWKH010000026         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_806407, whole genome shotgun sequence.
ACCESSION   DASWKH010000026 DASWKH010000000
VERSION     DASWKH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806407"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..903)
                     /locus_tag="VGA92_01970"
     CDS             complement(103..903)
                     /locus_tag="VGA92_01970"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9845214.1"
                     /translation="MISGKTIAITRSKEDSKEFIELATKENAIPMPLPTIELVSKGDK
                     IVDEFLSDIAEFSPDYSVFMSSKAVKLLFDVAKNNSKHSQLQLAVANTIVMAVGPKTK
                     AALESEGIRVSYMPERYSSVGVGETFTKLNAEGKKVIIPRSGASTEFLAKLLQKIGLV
                     VKENYLYDVQAFSDTAQWQEFKETFSKNKVDGIVFTSASSVRGFFEIMSEEFEQADLL
                     RRLQKIQIVAIGPFTADELKKFNAKPKVADVHTVPGAFEAMKNIFSLA"
     gene            complement(900..1643)
                     /gene="cobA"
                     /locus_tag="VGA92_01975"
     CDS             complement(900..1643)
                     /gene="cobA"
                     /locus_tag="VGA92_01975"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX9845215.1"
                     /translation="MSGVVYLVGAGPGDPKLITMRAVELIKKADVVLYDRLVSKKIIS
                     MIPKKADKVYVGREVGDDYKHQDATNQMMIKYAKSHKHVVRLKGGDPFIFGRGGEEAE
                     YLRKHKIRFEIIPGITSGIGSAVYCGIPLTHRKYSSSVVFVTGHEDPQKSKEQVKWKA
                     LAKSADTIVIMMGLSRIGVISKNLIAGGLGKNTPVAVIQNGTSKNQRMIIGTIANIVD
                     KIKQNKITPPSIIIIGDVVKLHKIVGWKK"
     gene            complement(1640..2572)
                     /gene="hemC"
                     /locus_tag="VGA92_01980"
     CDS             complement(1640..2572)
                     /gene="hemC"
                     /locus_tag="VGA92_01980"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9845216.1"
                     /translation="MKLILGTRGSQLSLAQTNWVVSELKKKNPDYDFEIKTITTKGDT
                     DARPLFTIDQKGIFEKEIDRAVVNKTIDFAVHSLKDIPSQLMEGLVLGCVPRRQDVND
                     VFISKQDYTLQTIPANSVIGTSSLRRAVQISRIRNDVTVKPIRGNIESRIKKIQDGNY
                     DAIVLAQAGINRLGVNVRYTKLPISEFSPSPGQGALGIVCRADDTRTLDMLKQIEDED
                     SRIAIESERTLSKFVESGCRFPVGALGRVNGASMTLTVVAYSIDGKKALVVEKTGPKN
                     EPQKLGQEAAEELRQKGVQELAADWREKLEEWNK"
     gene            complement(2569..3846)
                     /gene="hemL"
                     /locus_tag="VGA92_01985"
     CDS             complement(2569..3846)
                     /gene="hemL"
                     /locus_tag="VGA92_01985"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX9845217.1"
                     /translation="MSRSKGLFGAAKKVIPSGVNSPVRYFEPYPFFVHRAQGSSIWDV
                     DGNRYVDFCNGYGALLLGHRNKDVISAVKKQLDIGTMYGAPTELEINLAKLITEIFPS
                     MQKLRVVNSGSEATMTAIRLARGITKKKKIIKFEGCYHGAHESMLVKAGSGSAHSGIS
                     ISDGVPQDFSKNTIVVQYNNIEQLESVISKNKDVAGVIVEPVLANMGLVLPQKNYLQE
                     MRKITKENDIPLIFDEIITGFRVSLGGAQEYFGIRPDITTLGKALSNGFVISAVGGKR
                     EIMDQLAPAGKVYEASTFAGNPVSVAAAISSIKTMTRLKSKVYPRLAKYCRVLSKTID
                     DVACDLKIPHQVNSLASMFQVFFTGSPVVDYASSKKSDTKKFHWLFHYLLKRGVFVAP
                     SQFETGFFSYMHTESDLRKAMDAYQYGLKMVKS"
     gene            complement(3905..>4187)
                     /locus_tag="VGA92_01990"
     CDS             complement(3905..>4187)
                     /locus_tag="VGA92_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX9845218.1"
                     /translation="TFADLIFGLGGDDIISGEKGNDCIFAGDGDDIIYGNDGSDTING
                     EDGADIIKGQSGDDTLTGLTGNDIIDGGDGNDSCIVTPTDNDILLSCES"
BASE COUNT          940 a          999 c          853 g         1395 t
ORIGIN      
        1 tcgagatttt tagctgtcat gcattacaga gcttcgttat gttataaata gattatacac
       61 tgcttactag ttgtctgacg gagtagggac agctaagata gcttaagcta atgaaaaaat
      121 atttttcatc gcttcaaatg cgccgggaac tgtgtgaaca tctgcaactt ttggttttgc
      181 gttaaatttt ttcagctcgt ctgctgtgaa cggccctatt gcaacgatct gtattttttg
      241 caaacgcctc agtagatcag cttgctcaaa ttcttctgac attatttcaa agaaccctcg
      301 aacagatgat gcgcttgtaa aaacaattcc atcaactttg ttttttgaaa acgtttcttt
      361 gaactcctgc cactgtgcag tgtctgagaa cgcctgcacg tcataaagat agttttcttt
      421 taccaccagt ccaatctttt gcaaaagctt tgccaaaaat tcagttgatg cgccacttcg
      481 aggtatgatg actttttttc cctcggcatt tagtttggta aacgtctctc ctacgccaac
      541 tgaggagtac ctctccggca tgtacgagac acgaatccct tctgattcta gtgcggcctt
      601 tgtctttggg cctactgcca tgactatggt atttgcaact gcaagctgta gctgcgagtg
      661 cttggagttg ttttttgcca catcaaagag cagttttact gcctttgagc tcataaacac
      721 cgaataatct gggctaaact cggcaatgtc tgagaggaac tcatctacta ttttgtcccc
      781 cttgctcaca agctcaattg tcgggagcgg cattggtatt gcattttcct ttgttgcaag
      841 ctcgatgaac tccttcgagt cctcctttga gcgcgtaatt gcgattgttt ttcctgaaat
      901 cattttttcc atcccactat tttgtggagt tttactacgt ctcctattat aattattgat
      961 gggggcgtga tcttgttttg ctttattttg tcgacaatgt ttgcaatagt accaattatc
     1021 attctttgat ttttcgaagt gccgttttgg attactgcga caggtgtgtt tttgccaagg
     1081 cccccggcaa tcagattttt tgatatcact ccaattcttg agaggcccat cattattact
     1141 attgtatcag cagattttgc aagcgccttc cactttactt gctcctttga cttttgtggg
     1201 tcttcgtgtc ccgtaacaaa cacaacagaa gacgaatatt ttctatgggt gagtggtatt
     1261 ccgcaataca cagctgagcc aatcccagat gtgatgccgg gaattatttc aaatcttatt
     1321 ttgtgttttc gcagatattc cgcttcctcc cctccccgtc caaagataaa cgggtccccg
     1381 cccttgagtc tgactacatg tttgtgggat tttgcgtatt ttatcatcat ctgatttgtt
     1441 gcgtcttggt gcttgtagtc atcacctacc tccctgccca catagacttt gtctgctttt
     1501 tttggaatca ttgagattat ttttttgctt accagtctgt catacagtac aacgtctgcc
     1561 tttttgatga gctcaactgc acgcatcgtg atgagctttg ggtcccccgg tcctgcacca
     1621 actagataca ctactccact catttgttcc attcctccag tttttctctc cagtcagctg
     1681 ctagctcctg aacccctttt tgtcttaact cctctgctgc ttcctggcct agcttttgcg
     1741 gctcgttttt tgggccagtt ttttcaacaa ctagcgcttt tttgccatca atggaatacg
     1801 ccacaaccgt aagagtcatg cttgcaccgt ttacgcgccc taacgcacca actggaaacc
     1861 tgcagccaga ctctacaaat tttgaaagtg tccgctctga ctctatagca attctcgaat
     1921 cctcatcttc gatttgtttt agcatgtcta gtgtcctagt gtcatctgca cggcaaacta
     1981 ttcccagtgc accctgcccg ggggatgggg aaaactcgga aattggaagc ttggtgtacc
     2041 tgacatttac tccaaggcga ttaattcctg cttgtgctaa aactattgcg tcatagtttc
     2101 cgtcttggat cttctttatt ctggactcta tgtttccccg gatcggcttt accgtaacat
     2161 cgtttctgat tcttgagatt tgtactgcgc gccgcaggct gcttgtgcca atcacagagt
     2221 ttgccgggat tgtttgcagc gtgtagtctt gttttgagat aaagacgtcg tttacgtctt
     2281 ggcgccttgg aacgcaccca agtacgaggc cctccattag ttgtgatgga atgtccttta
     2341 ggctgtgcac tgcaaagtct attgttttat ttacgactgc cctgtctatt tctttttcaa
     2401 atatcccttt ctgatctatg gtgaagagcg gtcttgcgtc agtgtccccc tttgtcgtaa
     2461 ttgtctttat ttcaaaatca tagtccgggt ttttcttttt cagctcagag acaacccagt
     2521 ttgtttgcgc aagcgacagc tggctccccc tggttcccaa tatgagtttc aacttttcac
     2581 catctttagg ccgtattggt atgcgtccat tgcctttcgc aggtcagatt ctgtgtgcat
     2641 atatgagaaa aacccggtct caaactgcga tggtgcaaca aagacgcctc gctttaatag
     2701 ataatgaaat agccagtgga atttttttgt gtcggatttt tttgagcttg catagtctac
     2761 gactggagaa ccagtaaaga atacctgaaa cattgaagct agggagttga cctggtgagg
     2821 aattttgagg tcgcatgcca catcatcaat tgtcttagac agtacacgac agtattttgc
     2881 aagccttgga tagactttgc tttttagcct agtcatcgtc ttaattgagc tgattgctgc
     2941 tgcgactgag actgggtttc ctgcaaaggt gcttgcttcg tacactttgc ctgcaggcgc
     3001 aagctgatcc atgatctccc gtttcccccc cactgccgaa attacaaatc cattgcttag
     3061 tgccttccca agtgttgtga tgtccggcct tattccaaag tattcctgcg ccccgccaag
     3121 cgatactcga aagccggtaa ttatctcatc aaagattaat ggaatgtcat tttcctttgt
     3181 tatctttctc atttcttgca aatagttttt ttgcggcaat accaagccca tgtttgctag
     3241 gaccggctcc acaatcaccc ctgcaacgtc tttatttttt gagatgactg attctaattg
     3301 ttcgatgttg ttgtactgca caactatggt gttttttgaa aaatcttgcg gcacgccatc
     3361 ggaaattgaa atcccactgt gtgcggaacc agagcccgcc tttacaagca tcgattcgtg
     3421 ggccccgtga tagcatcctt cgaacttgat tatttttttc ttttttgtaa ttcctcttgc
     3481 aagtcttatt gcagtcattg tggcctcaga gccgctgttt accaccctga gcttttgcat
     3541 tgatgggaat atttctgtga tcagctttgc aagattgatt tcaagctcgg ttggcgcccc
     3601 atacattgta cctatgtcta gttgtttttt gacggcagaa atcacatctt tgtttctgtg
     3661 gccaagtaat aacgccccat aaccgttgca aaaatccacg taacgattgc catccacatc
     3721 ccaaatcgag ctgccctgtg cccgatgtac aaagaacgga tatggctcaa agtaacgaac
     3781 tgggctgttt accccagacg ggattacctt ttttgctgcg ccaaagaggc cttttgatct
     3841 cgacaacgat ttgacataat tttttgttat tattaatcct aaatgttatt gcgtcagcct
     3901 aagattagga ctcgcagcta agtagaatgt cgttgtctgt tggtgttaca atgcaggaat
     3961 cattgccgtc gccaccgtca ataatgtcat tgccagtcaa tccagttagt gtgtcatcac
     4021 cagactggcc tttgattatg tctgcgccgt cttcgccgtt aattgtgtca cttccatcgt
     4081 tgccgtaaat gatgtcgtcg ccgtcaccag caaagatgca gtcgtttcct ttctcgccag
     4141 atatgatgtc atcgcctccg agtccgaata tcagatcagc aaaagtc
//
LOCUS       DASWKH010000027         5429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_68312, whole genome shotgun sequence.
ACCESSION   DASWKH010000027 DASWKH010000000
VERSION     DASWKH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68312"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGA92_01995"
     CDS             <1..379
                     /locus_tag="VGA92_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6775 family putative metallopeptidase"
                     /protein_id="HEX9845219.1"
                     /translation="RALIGANPYIISTTGIIEAPAKPRQYYLDLMVNYGQGLNIDSIK
                     QKYQGTYLEYHDSRLGKIVEGYCLQALFYHITGDPFCDLLECRLNNAHWQRDLLYSQL
                     EFGKMCTKHTQILEEWTLHNLNC"
     gene            395..1381
                     /gene="trxB"
                     /locus_tag="VGA92_02000"
     CDS             395..1381
                     /gene="trxB"
                     /locus_tag="VGA92_02000"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9845220.1"
                     /translation="MMMADSGATVVVEKDLPKSEKKKTLYDVIIIGAGPAGYTAGIYC
                     ARARHETLILSGILPGGQLVNTTDVENYPGFENGIMGPDLMVTMRKQTQRMGAMIIDD
                     EVVNVDFRRKPFKILTASEEYECKAVIIATGASPRKLGLSGETTFGGRGVSYCATCDG
                     PFFRNQELIVAGGGDSAIEEATFLTKFASKVHLVHRRDQLRASKVMQERAFSNPKIQF
                     HWDTEVVDIIGDQKVQKAALKNIKTQEKTVLDVGGIFVAIGHTPNTKIFEDQVEIDPQ
                     GYIVLKDHTHTSVQGVFAAGDVHDRRYRQAITAAGFGCMAAIDVDRYITESK"
     gene            1470..2897
                     /locus_tag="VGA92_02005"
     CDS             1470..2897
                     /locus_tag="VGA92_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9845221.1"
                     /translation="MLFGKTGFKISGTVGIIILLLGITILFGTYQMSKVSGEIVMISQ
                     SYEPLQNILSDIKFHQSNQMNSFEKIKSVSQDGGDSENALETFWSSNVLTKSGIQQAK
                     KITEASYSIAPTESTENNFKLIHQTILDIEQMRDEFEELTKSSLSSTQNDQFLINQIA
                     VKERQITSKVDFASLEIKKFNEQSITVIEEYERDWLFVQIGIIVVVGIIAIFLRYFIS
                     QINNDLKKEIEEKTHELQEANKKLQELDMRKNEFISIASHELKSPIQPIFGFAELAQS
                     GDMDQKEAWDGVITLAKKLQDLATDVLDVTRIESNRLILYPQKFAINEIILETTKMIR
                     TGLTKDIKIIEDLGENIVIEADKTRIEQVLRNILDNAIKFTENGTVTVKTHLDKKAGE
                     LIFTISDTGLGIPEDILPNIFGKFVTKGHEMENQSGNGLGLFLCKGIINAHDGRILAH
                     NNKGSGSTFEFSLPLILKKPTPLTN"
     gene            2970..3353
                     /locus_tag="VGA92_02010"
     CDS             2970..3353
                     /locus_tag="VGA92_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845222.1"
                     /translation="MNILVAEDNVFTATQYTKALEKNGHKVTIAKDGQECLQLYDNEI
                     AKSEFNSLEKNPFDVVLLDNNMPKKSGVEVAKEILDKRPNQRIIFASAYDINSLLKAP
                     GNIPETVEILEKPFSLNTMISKVQT"
     gene            complement(3354..4352)
                     /locus_tag="VGA92_02015"
     CDS             complement(3354..4352)
                     /locus_tag="VGA92_02015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX9845223.1"
                     /translation="MATKVLTDTDTLKKIGNTPIVKLESLSNNKVTYYAKLEGHNPFG
                     SVKDRAAFWMIKDAEEKGKIKRGQTIIIEPTSGNTGIALAGIANLLGYQVEVVIPEKA
                     SEETKKIIASLGAKIYETSDDLCPKVGAGTDQSIALATSIASSRPDKYFSPNQYSNEA
                     NFLGHYKGTGPEIWKQTEGKITHFFTGVGTGGTVTGISTYLKEQNQKIKTIGVQPQQN
                     HLIQGLRNFEESAKPDLFLRRENVVDDWITITNEQAFAAVKEIFQKEKMLVSPSSATV
                     YAAMKKYQISEGIVVGIFADDGRKFKSLYAQQKVFSEEEFDRALKEAKHVSDIVYL"
     gene            complement(4356..4589)
                     /locus_tag="VGA92_02020"
     CDS             complement(4356..4589)
                     /locus_tag="VGA92_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX9845224.1"
                     /translation="MSTTTKKLDARGLFCPEPVFRTKIEVERMQVGETLTVLADDPNA
                     EDDISRWVKRNGHELLSLQKRESELEFSIKKVK"
     gene            complement(4689..>5429)
                     /locus_tag="VGA92_02025"
     CDS             complement(4689..>5429)
                     /locus_tag="VGA92_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX9845225.1"
                     /translation="QMLEYKNNHAKYKQIQVYPAEEITTSTGAHVIAYGITKEIKAGM
                     SLEEVLDEIKKQDAISCAPHPFSLLDALREKAGLCDLVEVFNSNNVDVISNARAAKFS
                     LEHNKVGVAGSDSHVLSTLGRCVNIIESENTLDTTLFALKHRKITIKQTGYAQEKETL
                     EHIKYKINNSKDYVAEYIRENYPNSQWILSLLLKMYDLNQNSYLWSLIYKLAVYLMKR
                     ISKKINLLNHDPSLLSERNLASMLRMAI"
BASE COUNT         1675 a         1036 c         1111 g         1607 t
ORIGIN      
        1 tagggcgctt attggcgcaa acccatacat catttcgaca actggcataa ttgaagcccc
       61 tgcaaagcca cggcagtact atcttgattt gatggtaaac tacggtcagg gcttaaacat
      121 tgattctatc aaacaaaaat atcaaggcac atatctagaa taccacgata gccgtcttgg
      181 aaaaatagtg gaagggtatt gcctgcaagc gttgttttac cacataacag gtgatccatt
      241 ttgtgatctt ttagagtgta gactaaacaa cgcccattgg cagcgggatc ttttgtactc
      301 gcaattagag ttcggcaaga tgtgtacaaa acacacacag attcttgaag aatggacttt
      361 gcataactta aattgttgag catttttagc ctcaattatg atggctgatt ctggcgctac
      421 agttgtagta gagaaggatc taccaaaatc agaaaaaaag aagacgttat acgatgtcat
      481 tattattggt gcaggaccgg cagggtatac tgctggaatt tattgtgcac gtgcaagaca
      541 tgaaactttg atattatctg gaatattgcc tggcggacag cttgtcaaca caacagatgt
      601 tgaaaactat ccgggatttg aaaatggaat aatgggacca gatctcatgg tgacgatgag
      661 aaagcaaact caaagaatgg gcgctatgat tattgatgat gaggtagtaa atgtcgattt
      721 tagacgcaaa ccattcaaga ttttgacggc atcagaagaa tacgagtgta aagcagtaat
      781 catcgcaaca ggcgcatccc caagaaaatt aggactctca ggcgaaacca cgtttggtgg
      841 aagaggcgtt tcttattgcg ctacgtgtga cggccctttt tttaggaatc aagaactaat
      901 tgtagcgggc ggaggagact cggcaattga agaggcaaca tttcttacta aatttgcaag
      961 caaggttcac ctagtccaca gacgtgatca gcttcgtgca agcaaggtga tgcaggaacg
     1021 ggcattttca aatccaaaaa tccagttcca ttgggatacc gaggtagttg acatcatagg
     1081 ggatcaaaaa gtgcaaaagg ctgcactcaa gaacattaag acacaggaaa aaacagttct
     1141 tgatgttggt ggaatttttg tggcaatagg ccatacgcca aacacgaaaa tttttgaaga
     1201 ccaagtagag attgaccctc aaggatacat tgtcctaaag gatcacacac atacaagcgt
     1261 gcagggggtt tttgcggcag gagacgttca tgaccgcaga tacaggcaag caatcactgc
     1321 ggcaggtttt ggatgcatgg cagcaataga cgttgacagg tatataacag aaagcaaatg
     1381 atttctgtct gttcgattta gacaaacact cacagaaaga attcatacgt tcagtcaatt
     1441 tgtgaagcaa gaccaatcaa gtgataatta tgctgtttgg taaaacaggg tttaagataa
     1501 gtggcactgt cggaataatc attttacttt taggaattac aattttgttt ggaacatatc
     1561 agatgtcaaa ggttagcggc gaaattgtta tgatttctca atcatacgaa cccttacaaa
     1621 atattttgag tgatatcaag tttcaccaat caaaccaaat gaacagtttt gaaaaaatta
     1681 aatctgtatc tcaggacggt ggagattccg aaaacgcgtt agaaacattt tggtcaagta
     1741 atgtcctgac aaaatcaggc atacagcagg ccaaaaaaat cacagaggct agctattcta
     1801 ttgccccaac cgaatctact gaaaataact ttaagctaat ccaccaaacc attttggata
     1861 ttgagcaaat gcgcgatgag tttgaagaac taacaaaaag ttctttaagt tcaacacaaa
     1921 acgaccagtt tttgataaac caaattgccg taaaggagag acaaatcaca agcaaagtag
     1981 actttgcatc attagaaatc aaaaaattca acgagcaatc aattactgtt attgaggagt
     2041 atgaaagaga ttggcttttt gtccaaattg gaataattgt agtagtggga ataattgcta
     2101 tttttttgag gtattttatc agccagataa acaacgacct aaaaaaagag attgaagaaa
     2161 aaacgcatga gttgcaagag gcaaataaaa agctacaaga gctagatatg agaaaaaacg
     2221 agtttatcag catagcatcg catgagctga aaagtcctat ccaaccaatc tttggctttg
     2281 cagagcttgc tcaatcaggg gacatggacc aaaaagaagc atgggatgga gtcatcacac
     2341 ttgcaaaaaa gttacaagat ttggcaacag atgttttaga tgtaacaaga attgagagca
     2401 atcgtctgat actatatcca caaaagttcg caattaacga gattatcttg gaaacaacaa
     2461 aaatgataag aactggattg accaaagaca tcaaaatcat tgaagatctt ggtgaaaaca
     2521 ttgtgattga ggcagacaag acaagaatcg agcaggtttt acgaaacatc ttggacaatg
     2581 caatcaagtt cacagaaaac ggcacagtta cagtaaaaac acacctagac aaaaaagcgg
     2641 gcgagttaat ttttacaata agtgatacag ggcttgggat acccgaagac atcctgccaa
     2701 acatttttgg caagtttgtt acaaaaggcc atgagatgga aaatcagagt ggaaacggtc
     2761 ttggcttatt tttgtgcaaa gggataatca atgcccatga cgggaggata ttagcacata
     2821 acaacaaagg ctccggatca acgtttgaat tttcattgcc attaattcta aagaagccaa
     2881 ctccccttac aaactaaccc tgtttgtgtg cttcaaacaa acttgtatta aattattgat
     2941 ggaaaacttg gaagcttgta gacatgccct tgaacatact cgtagcagaa gacaatgtat
     3001 ttacagccac ccaatacacc aaagcccttg aaaaaaatgg tcacaaagtg acaatagcaa
     3061 aggacggcca agaatgccta caattgtatg ataacgagat tgctaaatca gagtttaact
     3121 cgcttgaaaa gaatcccttt gatgtagtgt tgcttgataa caacatgcca aaaaaaagtg
     3181 gagttgaagt agcaaaggaa atcttggaca aaaggccaaa ccaacgaatc attttcgcat
     3241 ctgcgtacga catcaattca ctcctaaagg cgccaggcaa cataccagag acagttgaga
     3301 tattagaaaa accgttttca cttaacacga tgataagcaa ggtacaaacc tagttacagg
     3361 tagactatat ctgagacgtg ttttgcttct tttagggccc tgtcaaattc ttcttctgag
     3421 aagacttttt gctgtgcata aaggcttttg aattttcgac catcatcagc aaagatacca
     3481 actactatcc cttcggagat ttggtatttt ttcattgcag catacactgt tgcagaagaa
     3541 gggctgacta gcatcttttc tttttgaaat atctccttta cagctgcaaa tgcttgctcg
     3601 tttgtaattg taatccaatc atcaactaca ttttcgcgtc gcaaaaacaa atctggcttg
     3661 gcagattctt caaagttgcg cagtccctga attagatggt tttgttgtgg ttgaacgcct
     3721 attgttttga ttttttggtt ctgttctttt aggtacgtgg aaatcccggt aactgtgcct
     3781 ccagttccaa cgcctgtgaa aaagtgagtt atttttcctt cggtttgttt ccagatttct
     3841 ggtcctgtgc ccttgtagtg tccaagaaag tttgcctcat ttgagtattg atttggtgaa
     3901 aaatacttgt ctggccttga cgatgctatc gatgttgcca acgctatgct ctgatcagtt
     3961 cctgcaccca cctttggaca caagtcgtcg ctcgtctcat atattttcgc tccaagagat
     4021 gcgattatct tctttgtctc ctcgcttgcc ttttctggaa tcacgacttc aacttgatat
     4081 ccaagaaggt ttgctattcc agcaagtgca attccagtgt tgccagatgt tggctctata
     4141 attattgtct gtcctctctt tatcttgcct ttttcttcgg catcttttat catccagaac
     4201 gcagctctat ctttgacaga accaaatggg ttgtggcctt ccagttttgc ataataagtt
     4261 accttgttat ttgataatga ttctaattta acaatcgggg tgttgccaat tttctttagt
     4321 gtatctgtgt cagttagaac tttggttgcc acagactact tcaccttttt gatggaaaat
     4381 tctaattcgc tttctctttt ttgtaaagac agcaattcat gaccatttcg tttgacccaa
     4441 cgtgagatgt catcttctgc atttggatcg tctgcaagaa ctgtgagtgt ctcacctacc
     4501 tgcatcctct ctacttcaat tttagttcta aagacaggtt ctggacaaaa caatccacgc
     4561 gcatctaatt ttttagtggt tgtactcatt gatatttttt ggaggctggt ttgtatatta
     4621 aaagctattc aacttagttt tagctaaatg tcttttttga atgcgtagtc tcttgttcat
     4681 gatgtaaatc aaattgccat tctaagcata ctagctaggt ttctttcgct tagtaaagaa
     4741 ggatcatgat ttagaagatt aattttcttt gagatgcgct tcatcagata taccgctagc
     4801 ttgtaaatta gcgaccatag atagctattt tggtttagat catacatttt tagcaagagc
     4861 gagagaatcc attgcgagtt tggataattt tccctgatgt attctgcaac atagtcctta
     4921 gagttgttta ttttgtattt gatgtgttca agtgtttcct tttcttgcgc atatcctgtt
     4981 tgcttgattg ttattttacg atgttttaat gcaaacagag tagtatcgag cgtattttct
     5041 gactcgatta tgttcacgca tctgccaaga gtggacagga cgtgcgaatc gcttccagcc
     5101 acaccgactt tgttgtgttc tagtgaaaat ttcgctgccc ttgcgttaga gatcacatcg
     5161 acattgttgc tgttaaagac ctcaaccaaa tcgcacagtc ctgccttttc gcgcaatgcg
     5221 tcaagaaggc tgaatgggtg aggcgcacat gaaatggcat cctgtttctt aatttcgtcc
     5281 agcacttcct ccaaagacat cccagctttg atttcctttg ttatgccata agcgatcaca
     5341 tgtgcccccg tacttgttgt gatctcctct gcaggataaa cctgaatttg cttgtatttt
     5401 gcatggttgt ttttgtactc taacatctg
//
LOCUS       DASWKH010000028         8321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3039005, whole genome shotgun sequence.
ACCESSION   DASWKH010000028 DASWKH010000000
VERSION     DASWKH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039005"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VGA92_02030"
     CDS             complement(<1..1259)
                     /locus_tag="VGA92_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845226.1"
                     /translation="MSKSPDLSVHLSQQITPYADVDVLTLSESVIIPDACKHMVRTNS
                     DEIIVVDSNGHPIGIVTDEDILKKTSEAFINPSNTTLGDVMTFPVISITQDKTLSDAL
                     DKMRQYKLRKLVVLSEQNRIVGMLYKNTIIDLMKKHLAIRQEKRSSLWAILWNLGIVL
                     QFAGVLMFVPAILATVLWETVPATGIYLMSTMLFVTGFFMNTYGEKYPLKLRDAAILV
                     FASFLILVLFGTIPHVLVVPFDADKPLEHFARSFFESSAGFTTGGLTTIPIPEELPQS
                     FTFYRSYTQFVGGLSFIYLIVTAFYPENKLHSMRSFISGRIPQLKELFITITILFSIY
                     ITIIAVALFYFGEANLIDNFALAMNAVSTGGWIPHSQILVDLTIPEYIVLMVGLIFGA
                     LPFGLHYAFVRTKFMSFQINQEVAIYLA"
     gene            complement(1398..2288)
                     /locus_tag="VGA92_02035"
     CDS             complement(1398..2288)
                     /locus_tag="VGA92_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX9845227.1"
                     /translation="MARGYQQEHVQQQLIEILLESKTGLSGVEIAEKLGLNRATMAKY
                     LNIFAAEGIIKQKNIGNANLWFVESGTETLEFPADYFHVKEKFLEYLTSGLQHQAYHL
                     IRNCQYSGADTSKIIGEVIIPAIASLEDMYLKAKIGKSEAKLFDGIISNSIQTLSLAP
                     YDFDPKKNLLTLAADSASVLYAQAAACVLSSKGWQGWPLGDMSDAIDVLYDLDLQKLL
                     TRIWKQKHGLMVVVIFSATEKGLKFFSESVNSVKPKFGKNLHLGVYSKAEKKNIKAEF
                     ASENFEAVLQWVESVSPSVS"
     gene            2395..2781
                     /locus_tag="VGA92_02040"
     CDS             2395..2781
                     /locus_tag="VGA92_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845228.1"
                     /translation="MPKAGFKSITVSEDVYDKFFDVFEKNKPELMMKGINSFSGYVTY
                     MLEQTMQKDKTFARYAPKIEKVSVDDDRVILKDNIKNRIAEVAIQRGELFCQLCEEKD
                     CVHIGYVFSLPDVYEVLNSKGIKHPK"
     gene            2821..3219
                     /locus_tag="VGA92_02045"
     CDS             2821..3219
                     /locus_tag="VGA92_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845229.1"
                     /translation="MSKEPRSVIVKTMVVDRPTDRVFDFFANPKNWESGGALKNIRPQ
                     SDGWWLADSPFGSSKFKLHPNKEAGILDHDFIGEGEWIVFVRITPNERGSTISWTFIR
                     PEAMIHERFECQLQNFDKEIEGWKKAIELT"
     gene            complement(3241..3365)
                     /locus_tag="VGA92_02050"
     tRNA            complement(join(3241..3288,3304..3317,3340..3365))
                     /locus_tag="VGA92_02050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3305..3307),aa:Leu,seq:taa)
     gene            3456..3529
                     /locus_tag="VGA92_02055"
     tRNA            3456..3529
                     /locus_tag="VGA92_02055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3490..3492,aa:Thr,seq:ggt)
     gene            complement(3534..4388)
                     /locus_tag="VGA92_02060"
     CDS             complement(3534..4388)
                     /locus_tag="VGA92_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX9845230.1"
                     /translation="MKQRQILTTEPKPFVKWAGGKRQLVPIIEKHIPQEFGTYYEPFL
                     GGGAVLFHLLCKKPDTKCHVSDLNSDLVLAYVTIRDRVEELISSLQTHAKNYHKSPDS
                     YYYSVREGQPKSQIEKVSRLLFLNRTCFNGLYRVNSKGKFNVPLGRYSNPNIVNDENL
                     VSVSHTLQSKKIQISCRDFSSIVSDAKKNDFVYFDPPYQPVSRTANFTSYTNRDFTYE
                     DLRRLVEVSERLADKGCKVLHSNSNSREVRDLFSRDWKIVEISANRAINSDSTKRTGH
                     TELLIKSY"
     gene            complement(4517..5167)
                     /locus_tag="VGA92_02065"
     CDS             complement(4517..5167)
                     /locus_tag="VGA92_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia monooxygenase"
                     /protein_id="HEX9845231.1"
                     /translation="MVWLRRCTHYLFIVVVAVNSTLLTINAGDYIFYTDWAWTSFVVF
                     SISQTLMLVVGACYYLTFTGVPGTATYYALIMTVYTWIAKGAWFALGYPYDFIVTPVW
                     LPSAMLLDLAYWATKKNKHSLILFGGVMCGLSLPLFNMVNLITVADPLETAFKYPRPT
                     LPPYMTPIEPAVGKFYNSPVALGAGAGAVLSVTMAALGCKLNTWTYRWMAAWSKWD"
     gene            complement(5291..5650)
                     /locus_tag="VGA92_02070"
     CDS             complement(5291..5650)
                     /locus_tag="VGA92_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845232.1"
                     /translation="MTLPKGFGSGGGGATSADVEKMIGRRVENMTGIITLSYIAAWLA
                     TFGGTAAGYFYYPWAYPTPSGHYAFIVLTIVEAIGYLFCVKVMEEGTKKKSNGIIGAT
                     LAATAIGTILISIFVGV"
     gene            complement(5750..6313)
                     /locus_tag="VGA92_02075"
     CDS             complement(5750..6313)
                     /locus_tag="VGA92_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit C"
                     /protein_id="HEX9845233.1"
                     /translation="MAQMPALIPKEVEIQRLKKIWLIVIAMGSTAASVEVDNFVDGSL
                     HQTSIRDSAFTPAHWWLYSHFVALPLGWGAVAIYDRRVPILRGPNNSMNTGLKMTILG
                     YLATMFTIGVNEMWHFWFVEEIFAVPNHWMFNMGVVVAFMGALAYVVRVYARLVELGA
                     ETPGENPYVAEMYKMALEGKLYSRSIP"
     gene            6597..7163
                     /locus_tag="VGA92_02080"
     CDS             6597..7163
                     /locus_tag="VGA92_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methane monooxygenase/ammonia monooxygenase
                     subunit B"
                     /protein_id="HEX9845234.1"
                     /translation="MVDKKVFVVALGVILALGTFGPNIAQMIQTAEAHGVQAQLQSRF
                     VKIEDETFNRQSLQTGEELVISGKFVSLVERDLRAWNSIFSESTNAGNRWEILARSPP
                     GNVFQIPGNAVIPYEIRAKALEPGVYHVHTQLNVASVGPGLGPGQTVVVSGDPIIKPI
                     PYTNIAYQSIIIGVGYVITFATRPWQVI"
     gene            7241..7759
                     /locus_tag="VGA92_02085"
     CDS             7241..7759
                     /locus_tag="VGA92_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845235.1"
                     /translation="MIWPGMGDPSKRRKTLKFLIITAAIGVGVAAASSGVQILLNTDN
                     PLKVCINDKEINYRVSATLEVYIDGQKADIPAKVGFKDGCQRALYTETPDGTIHAAWS
                     EQYPFELGHFLWLWEFPLRDMNEAKSTIFVNGETSSDFIRTPLVDGNHYRAEFTSKAY
                     DESQEKDFLPPQ"
     gene            complement(7820..8089)
                     /locus_tag="VGA92_02090"
     CDS             complement(7820..8089)
                     /locus_tag="VGA92_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845236.1"
                     /translation="MTPLLGTVFSILSTVFGQLGPNYDPLFYDVMVYIFGTMMFTVFL
                     LGIIAYWLRVHGWSYKDNRERWVDELDKHFEREGIGLIPDNGKYW"
BASE COUNT         2440 a         1790 c         1766 g         2325 t
ORIGIN      
        1 gcgagatata tggcaacctc ctggtttatc tgaaaagaca tgaattttgt tctgacaaaa
       61 gcataatgaa gtccaaatgg aagtgcccca aagatcaatc caaccatgag cacaatatac
      121 tctggaatgg taagatcaac tagaatctgg gaatgcggta tccacccgcc agtcgatacg
      181 gcgttcattg ccaaggcaaa gttatcaatg aggtttgctt ctccaaaata aaatagcgcg
      241 acagcaatga tcgtaatgta gattgaaaaa agaatcgtaa ttgtgatgaa cagttctttg
      301 agctgtggaa tccttcctga gataaagctc ctcatggagt ggagtttgtt ttctggataa
      361 aatgcagtaa caatcagata gatgaaactc aatcctccaa caaactgtgt gtagcttcta
      421 tagaaggtaa agctttgagg cagctcttct ggaattggta ttgttgttag tccgcccgtt
      481 gtgaatccag cagagctttc aaaaaagctc ctagcgaaat gctcaagagg tttgtcagca
      541 tcgaatggaa ctaccagtac gtgaggtatg gttccaaaca gtaccagaat cagaaagctt
      601 gcaaacacca gaattgcagc gtcccttagc tttagcgggt atttttcgcc atacgtgttc
      661 atgaaaaatc ccgtcacaaa caacattgtg gacatcagat agattccagt tgcagggacc
      721 gtttcccaca gtactgtggc aagtattgct ggaacaaaca tcagaactcc tgcaaactgc
      781 agtacaattc ccagattcca gagtatggcc caaagcgagg atcgtttttc ctgccttatt
      841 gctaggtgtt ttttcatcaa gtcaatgatt gtgtttttgt agagcattcc aacaatcctg
      901 ttttgctctg aaagtacgac gagttttctt agcttgtact ggcgcatctt atcgagtgcg
      961 tccgatagtg tcttgtcttg agttatggaa atgactggaa acgtcatgac atcacccaaa
     1021 gttgtgtttg atggattgat gaacgcctcc gatgtttttt tcagtatgtc ttcgtcggtg
     1081 acgattccaa ttgggtgtcc attactgtca acaacaatga tttcgtctga attggttcta
     1141 accatgtgct tgcacgcatc tggaataatg actgactcgg aaagcgtgag gacatctacg
     1201 tcagcgtagg gcgtgatctg ctggctgaga tggactgaaa gatcaggcga cttggacatg
     1261 attacttcaa cgccaagatc tgttcttgat taatgttatg agattccttg atttagtgga
     1321 aattgtggtt ttaaataaag cgtgaacggc tgcatgactt gtagctttca tattcatcac
     1381 atcattttga gatttcatta tgataccgat ggagaaacag actcaaccca ttgtaacact
     1441 gcctcaaagt tctcagatgc aaattccgcc ttgatgtttt tcttttctgc cttggaatag
     1501 accccaaggt gcaggttttt gccaaacttt ggtttaacag agtttacaga ttccgaaaaa
     1561 aacttgagtc ctttttctgt tgcagaaaag atgaccacta ccatcagccc gtgtttttgc
     1621 ttccagattc tagtcaaaag cttctgcaaa tcaagatcat aaagaacgtc gattgcatcc
     1681 gacatatccc caagtggcca accctgccaa ccctttgaag aaagcacaca tgcagctgcc
     1741 tgcgcataaa gcacgctagc cgagtctgct gcaagtgtaa gtaggttttt tttgggatca
     1801 aagtcgtatg gggcaagact gagggtttga attgaatttg atattatacc gtcgaatagc
     1861 tttgcctctg attttcctat tttcgccttc agatacatat cctcaagtga ggcaattgcc
     1921 ggaattatca cctcaccgat aattttcgat gtgtctgccc cagaatactg gcagtttcga
     1981 ataagatggt acgcctggtg ctgcaggccc gaagtaagat attccaagaa tttttctttg
     2041 acgtggaaat agtctgctgg gaattcgagc gtttccgttc cagattctac aaaccataaa
     2101 tttgcattgc caatgttttt ttgttttatg atgccttcgg ctgcaaatat gttcagatat
     2161 tttgccatcg ttgcacggtt gagtccaagt ttttctgcaa tctccactcc tgataaaccc
     2221 gtctttgatt ctaataggat ctcaattagc tgctgctgca catgttcttg ttgatagcct
     2281 cttgccaaag ttgattttac tacgagtgct aattatttat gctgtaatca aggctttacg
     2341 tacaaaaaac attaaatagt attgtacctt aaagtacggt aataggtaag taaaatgcca
     2401 aaagcaggat tcaagtcaat caccgtttca gaagatgtct acgacaagtt ctttgatgtc
     2461 tttgagaaga acaagccgga gctgatgatg aaaggcatca acagcttttc tggttatgtc
     2521 acctacatgc tagagcagac gatgcaaaag gacaagacct ttgcgcgata tgctccaaaa
     2581 atagaaaaag tatcagtaga cgatgatcga gtcatactaa aggacaacat caaaaacaga
     2641 attgcagaag ttgccataca aaggggagaa ctattttgcc agctttgcga ggaaaaagac
     2701 tgcgtccaca ttggctacgt gttctcactt cctgatgtct acgaggtctt aaactcaaag
     2761 ggcatcaaac acccaaagta acttggcata atttttatca gttccacaac tggacaaccc
     2821 atgagtaaag aacccagatc cgtgattgta aagaccatgg ttgtcgacag acccacagat
     2881 agggtttttg atttttttgc aaatccaaag aactgggaat ctggaggtgc cctcaaaaac
     2941 atcagacccc aaagtgacgg atggtggctt gcggactcgc cttttggcag ctcaaagttc
     3001 aaactacatc caaacaaaga ggccggaatt ttagatcatg attttattgg tgaaggagag
     3061 tggatagttt ttgtaaggat tactccaaac gaaagaggtt ccactatttc atggacattc
     3121 attaggcccg aggcaatgat ccacgaacga tttgagtgcc aactgcaaaa tttcgataag
     3181 gaaatcgagg gctggaaaaa agccatcgag ctgacataat tttttttggt gtggataaaa
     3241 tggtgcaggg agtgggattt gaacccacga acccctaaga gacgggatca cccattatta
     3301 gatcttaagt cccgcgcatc caaaagcaag ctttgctttc tttggccagg ctcggcaatc
     3361 cctgcggatt agctaaaatt tggctgagat ttaacagttt agcgttgccg aaatttttga
     3421 aagaataata aggattcgct ttagcaaaaa tttgtgcttc gatagctcag cctggtagag
     3481 cgttaccttg gtaaggtaaa ggtcgcgggt tcggatcccg ttcgaagctt tttctaataa
     3541 ctttttatca aaagttctgt gtgcccggtt ctttttgtag aatcagagtt gatggcgcgg
     3601 tttgccgaaa tctccactat cttccagtcc cttgaaaaca gatcgcgaac ctccctcgag
     3661 tttgagtttg agtgcaagac cttgcagccc ttgtctgcca gcctttcaga tacctcaact
     3721 agccttcgca gatcctcgta ggtaaagtcg cggtttgtgt agctcgtaaa gttggcagtc
     3781 ctgcttaccg gctggtacgg aggatcaaaa tacacaaaat catttttttt ggcatcagat
     3841 acaattgagg aaaaatcccg gcagctaatc tggatttttt ttgactgcag agtatgactt
     3901 actgagacca agttttcatc atttacaatg ttcgggtttg agtacctccc aagcgggacg
     3961 ttgaacttgc ctttgctgtt gacacggtac agcccattaa agcaggtccg attcaaaaac
     4021 aacaatcttg ataccttttc aatctggctt tttggctggc cttctcttac cgaataataa
     4081 tacgaatccg gacttttatg ataatttttt gcatgagttt gtagcgatga gattagctcc
     4141 tcgactctgt cacgaatggt aacatacgcc agcacaaggt cagagtttag atcagacaca
     4201 tgacatttgg tgtcaggttt tttgcagagt agatgaaaca acactgcccc tccgcccaaa
     4261 aacggctcat agtatgtgcc aaattcctgc gggatgtgtt tttcaatgat tgggactagc
     4321 tgcctttttc ctccggccca cttgacaaag ggctttggct ctgtggttag tatttggcgt
     4381 tgtttcaata atacatgatt tcaaaaaatc atatcccaaa catagacaac aaaagatcac
     4441 aacctatgca atatttttta atcaaaacct cgcggtagcg ctcaaaaaat aagataaagg
     4501 aaaaaggtgt tgggttttag tcccacttgg accaagctgc catccatcta tacgtccacg
     4561 tgttcagctt acatcccaga gcggccatgg taactgataa tacagcacct gcgccagcac
     4621 ctaatgcaac cggactgtta tagaacttgc ctactgcagg ttctatcgga gtcatgtatg
     4681 ggggcaaagt tggtcttgga tatttgaatg cagtctctaa tgggtcagca accgtgatta
     4741 gatttaccat gttaaacaat ggtaacgaca gaccgcacat tacaccacca aatagtatca
     4801 gtgagtgctt gttcttcttt gtagcccagt acgccaaatc caacagcatt gctgatggca
     4861 gccaaactgg tgtgacaatg aagtcatatg gataacctag tgcgaaccat gcgcctttgg
     4921 caatccacgt gtacacggtc atgatcaacg cgtaatacgt cgctgttcct ggaacgcctg
     4981 taaatgtcag atagtagcat gcgccaacta ctaacattag tgtctgcgat atagagaaga
     5041 caacaaacga agtccaggcc caatcagtat agaagatgta gtcccctgcg ttaattgtta
     5101 gcagagtaga gttgactgca accactacta tgaataagta gtgagtacat cgtctaagcc
     5161 agaccattaa tcccctaggg aggggctgaa tatataaaat tttatgcgcc tgtgtagatc
     5221 ggagacctaa atttcggcaa aatattacga ataggggtcg aattaaaaat aagaaaaaga
     5281 ctggagagtc ttatactcca acgaatatcg agattagtat agttccaata gcagttgcgg
     5341 ctaatgttgc gcctataatg ccattgctct tctttttggt gccctcttcc atgaccttta
     5401 cgcagaagag ataaccgatt gcctcgacta ttgtcaggac tatgaaggca tagtgaccgc
     5461 ttggtgttgg ataagcccat ggatagtaaa agtatccagc agctgttcca ccaaaggttg
     5521 caagccatgc agcaatgtat gaaagcgtga ttatgccagt catgttttct acacgacgac
     5581 caatcatttt ctctacatca gcactggtcg cgccaccgcc acctgagcca aatccttttg
     5641 gtagagtcat ttgccatact aatcgataga atacttttaa gcctttcgta aaaaccgggc
     5701 atatcccgat ctagcatatg aatgaaaaat gagaaaaagg gagttttgtt tatgggatgc
     5761 ttctgctgta caatttgcct tctagggcca tcttgtacat ttcagcaaca tatgggttct
     5821 cacctggtgt ttctgcacct agttcgacta gccttgcata gactctaacg acgtatgcaa
     5881 gagcgcccat gaatgctact actacaccca tgttgaacat ccagtggttt ggtactgcga
     5941 agatttcctc tacaaaccag aagtgccaca tttcattgac tccgattgtg aacatggttg
     6001 caaggtaacc aagaatggtc atcttgagtc ctgtgttcat cgagttgttt ggacctctga
     6061 gtattggtac tcttctatca tagattgcta ctgcacccca acctaatggt aatgcaacaa
     6121 agtggctata caaccaccag tgtgctggag tgaatgcaga atctctaata gaggtttgat
     6181 gtaaagaacc atctacgaag ttatctactt ccacggatgc tgctgttgat cccattgcaa
     6241 taacgatgag ccagattttc tttagtctct ggatctcaac ttcttttggg attagtgccg
     6301 gcatctgtgc cataattcaa caaaggatac tttcaatata taaaagatga gtcaatttgg
     6361 gccaaaattc attgtaaatt ttacataaaa tccttagaat tattatacgt cttgcataac
     6421 cacaccaaca tagataaaaa ttccacaaaa cactattttt atggtttcaa tgttcaacat
     6481 tgatgtagaa caatgagatc cgtttttgca gtcaggattt ttgagatccc cattgcaccg
     6541 caacggtaaa acatatatcg ttctatgttg agtttttgaa tattaaaggg ataactatgg
     6601 tcgacaagaa agtattcgta gtagcactcg gtgtcattct ggcacttgga acatttggtc
     6661 ctaatattgc ccagatgatt caaactgcag aggcacatgg tgtacaagca caactacaga
     6721 gtcgttttgt aaaaatcgaa gacgagacat tcaacagaca gtcactgcaa acaggtgaag
     6781 aactagtcat ctctggcaag ttcgttagtc ttgttgaaag agacctaagg gcatggaact
     6841 cgatattctc agagtcaacc aacgcaggta acagatggga aatccttgcg agaagcccac
     6901 caggaaacgt ctttcagata ccaggtaacg ccgtaatccc atacgagatc cgagcaaagg
     6961 cactcgaacc aggcgtatac cacgtacata cacaactcaa cgttgcctca gttggaccag
     7021 gattaggccc aggacaaaca gtagtagttt ccggcgatcc aataatcaaa ccaattccat
     7081 acacaaacat tgcataccaa tcaatcatca ttggagttgg ctacgtcata acatttgcaa
     7141 ctcgcccctg gcaagtaatc taaaccaaca caccctctct tttcatttta ttatggtctc
     7201 ctagagaaac gactttatgt tattccagtc cagttcagcc atgatttggc ccggaatggg
     7261 agacccaagc aaaagacgca agaccctcaa atttctcatc atcactgccg caatcggagt
     7321 cggtgttgca gctgcaagtt ccggagtcca gattctgcta aacactgaca atcccctcaa
     7381 agtctgcata aacgacaagg agatcaacta tcgggtttct gccacactcg aagtctacat
     7441 cgacgggcaa aaggccgaca ttccagccaa ggttggcttc aaggacggct gccagagggc
     7501 gctatatact gagactccag atgggacaat ccatgcagca tggagtgagc aatacccatt
     7561 tgagctaggc cactttctat ggctctggga gtttccccta agggacatga atgaggccaa
     7621 gtcaacgatt tttgtaaacg gggagacatc atctgacttt atccgaaccc cactagttga
     7681 cgggaatcat taccgcgccg aatttacatc aaaggcatac gacgagtccc aagaaaaaga
     7741 ctttctgcca ccgcaataag tcggcaaaag acaccctaac gactaagata aaaaactaaa
     7801 aagaatgaaa aaagaagttt taccagtatt tgccgttgtc tggtatcagt ccgatacctt
     7861 ctctctcaaa gtgtttgtcc aactcatcaa cccatctttc gcggttgtct ttgtatgacc
     7921 agccgtgaac acgtagccag tatgctatga ttccaagaag gaaaacagta aacatcattg
     7981 tgccaaagat gtaaaccatg acgtcataga ataatgggtc atagtttggc cctagttgtc
     8041 caaatacggt agacagaata ctaaacactg taccgagtaa tggagtcatt gaattactaa
     8101 cctaataatc tgcgatatat acatttccct gatagatgct gtcagaggaa cggttgattt
     8161 gatcggcttt tcctctgtaa aaagtcggtt gaaatggctt ttttgccaaa aatcaaccga
     8221 aaatcgacaa gaacatggac tttcagaaac attgggtatt ttattgtagc aatgacagat
     8281 cccatagcca ttattggctt tattgtgtat ggtattgtta t
//
LOCUS       DASWKH010000029        44727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_810546, whole genome shotgun sequence.
ACCESSION   DASWKH010000029 DASWKH010000000
VERSION     DASWKH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 44727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44727
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810546"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGA92_02095"
     CDS             complement(<1..210)
                     /locus_tag="VGA92_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9845237.1"
                     /translation="MIYLDNAASTPVRDDVLQEMMPYFKENYGNPSSIHRFGRIATKA
                     IETARKRIAELINAQPSEILLTSGGT"
     gene            complement(207..1007)
                     /gene="larE"
                     /locus_tag="VGA92_02100"
     CDS             complement(207..1007)
                     /gene="larE"
                     /locus_tag="VGA92_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX9845238.1"
                     /translation="MNKIEQLKDWFSDKQGVIVALSGGVDSALVAYAAHGKLGSAALA
                     VTADYKTLAQEELESAKKICQEIGIRHLIISYDELENEDFTKNDKERCFHCRTELSEH
                     LKEIAQKENVQLVVDGTNIDDLGDYRPGIQALKNNGIQSPLVQVGLSKQEIRRIAKLV
                     GLSVFDRPSNSCLASRIPWGQRVTAEKLARIEVGEIFVKQILGAKQVRVRDIGGSAKI
                     ELGRDELSLFAEKTKLDELIAKLKLIGFDSVTVDEDGYRPGKINVIAD"
     gene            complement(1004..2230)
                     /gene="larC"
                     /locus_tag="VGA92_02105"
     CDS             complement(1004..2230)
                     /gene="larC"
                     /locus_tag="VGA92_02105"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX9845239.1"
                     /translation="MVLVIDPQIAGVSGDMILSSLVHLGANKAKIIDGVNTSQSYLHG
                     SKIKKIDFRRVNKHGIKATSLVLQLDEHNHKRKGIEIQNCIGKTAQKLGLSEQASRFA
                     INSINTLIAAESKIHGVPKDSVHFHEASSIDTVIDIVGTAIALDDLGHFDDEIITLPV
                     AVGSGTVSFSHGTVSNPAGAILGIFEKSGILIHGSKVRDELTTPTGASILVNLAEKCV
                     EFYPMMKVSSIGYGAGQKNFEGFSNVLKIVQGQSTSGLESDSVAILETNVDDVSGEVL
                     AHVIDKVISRGAKDVSVIPTITKKGRPGHIISVICDSNHVNQLLDVLVSETGTLGVRI
                     RTSDRLIVPRQILRMKIQARNQNFTVRCKVAKSGSNRKQFKVEYDDIKKISEKLALSF
                     KQTEELIKEQVKRQLK"
     gene            2341..3249
                     /locus_tag="VGA92_02110"
     CDS             2341..3249
                     /locus_tag="VGA92_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9845240.1"
                     /translation="MITIIGSGKVGGDAALFSALKKIDTEILLLDVVKGLPQGEAMDL
                     NHMLSEQGIDVSIRGSNDYSDMKGSDVVVVVAGSGRKPGMTRMDLLKINASIVKDVVE
                     NIKKHAKDSIIVPVTNPLDPMVHITYKTSGFERSRIIGMGNMLDLSRFIQFIHEATGY
                     SRDSIRALVIGEHGENMLPLPRFSTVAGIPLANILPKDKLDQLIQDTRGVAAKVIELK
                     GATVHAPGNAISSIVEAILRDKKKVIPVATPLDGEYGHSDVSIGVPAVVGKDGIEKII
                     ELDLNQQEKEWFNKGIDSVKGALSGI"
     gene            3286..4056
                     /gene="larB"
                     /locus_tag="VGA92_02115"
     CDS             3286..4056
                     /gene="larB"
                     /locus_tag="VGA92_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX9845241.1"
                     /translation="MEILDVLESLKSGKISVTEAKKLLSLYSIEKIEDFAQIDMGRRY
                     RKGVPEVIFAERKQLDEIKKIITKILGKSNSVLVSRIQKQDYSKIIAFAKKNKFKIKD
                     GKNTTALLLYKKEQKRSGGMVGIVTAGTSDIGVAEEARLTCEAMNCTCICSYDVGIAG
                     LHRIFPIIKKFVQQNVDVIVVAAGMEGALASVVASLVDVPVIGLPTSVGYGYGEKGVA
                     ALASMLQSCSLGLSVVNIDNGIGAGAVAANIANNAKRQ"
     gene            complement(4043..4765)
                     /locus_tag="VGA92_02120"
     CDS             complement(4043..4765)
                     /locus_tag="VGA92_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9845242.1"
                     /translation="MKDMSEPRHTEPHLSESSGIRDFVFGFGDGINTSLGIAAGVGGA
                     DVSSNFIILAALVGMFTGAKAMAVQNYLAVKAHRELLKSEIAREEWEIENKPDLERQE
                     IEDIYKAKGFSGKELEMVVNKITSDKKVWLDTMLTEELKLNVEVLGNPLKSAFRMFGA
                     FLVGGILPIIPFFFLDGYVPLIIAIGISLSTSFLVGAVKSKIANTNILKGGLEMAGLG
                     TGIALVGFGIGTELGNLGIIDV"
     gene            4910..6436
                     /locus_tag="VGA92_02125"
     CDS             4910..6436
                     /locus_tag="VGA92_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9845243.1"
                     /translation="MTNYRGNFLYGFIACGPYELLPEWVFDKVFCPAVETDPNTGEAK
                     VAQVGLRRVESALLQGYKRDEVFVANPDYLEKSIGPDTKVVGINVMDPLGMAPVTTTM
                     SPEKLSYVAMKFKRMCAKIIQLKKKYNFHVVVGGNGAWELAKTDRMRIHGLDTVVVGE
                     ADELALDLFKDLENGDAPELMHCFVKNIQNIPIIHGPTVNSLIEAMRGCGRGCDFCDV
                     NKRSKKDLPLERLQYEAKINLDYGFDSIWLHSDEMLLYGCDNKDFQPNRDAITELWRG
                     LKSLGANFVGTTHMTFSAVAADPELLKQMSEINNMHTTGRWLATNLGIETVAPRMVKK
                     HLGVKTKPFSTDEWGWVVREGARVMNENHWFPAATLIIGWPDETPDETQYTIDLIEDF
                     RKTSFRGLVAPLLYQDFSEKNSMHFGNLNEAQFTLFWRCWENNLRVINDIVPIILRNK
                     TYGPPMKVFMYGLIKAGTWAIMRYLRGLCKDLFNGKLPEDIVEKYARSRSITAPAYTR
                     "
     gene            complement(6440..6763)
                     /locus_tag="VGA92_02130"
     CDS             complement(6440..6763)
                     /locus_tag="VGA92_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845244.1"
                     /translation="MHVEYEEFASIEDIFSYMASVMPPMKNAMPINSYKGYVFAFIPL
                     THSNGDVYLMIYTKGTLDGRILEFDINTNTYRKVSAIERADKTYFIVLTPKRNTIADE
                     AIKQI"
     gene            complement(6795..7889)
                     /locus_tag="VGA92_02135"
     CDS             complement(6795..7889)
                     /locus_tag="VGA92_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEX9845245.1"
                     /translation="MKGAGILAVVFLSLMITSSALPTVQGQVDGSQIPPLSVKTELPL
                     YDQGDSVVFNGFVKNLDLNNLVDVTVRVIGPLVNGTSSNVVQVKQIKPQLDGSFEGSF
                     IVSGPLWQKKGDYQIIVNYGPQKAQTTFFYNGGTGSTDVDVPPPPPPPKCGEGEILKN
                     NQCVPFETTPQCGPGTVLDTVTGLCVVAPPVDCPPGQMLVGGQCIDEPPKDTGPECGP
                     GTVAKDGICVPEVTPPPPTNGCLIATAAFGSELAPQVQLLREVRDNVLFGTSSGTSFM
                     TGFNSVYYSFSPTIADWERNNPVFKEVVKTAITPMLSTLSILNYVEIDSEQEMLGYGI
                     GIILLNIGMYFVAPAIAIVKLRGIISKKHL"
     gene            complement(8061..10982)
                     /locus_tag="VGA92_02140"
     CDS             complement(8061..10982)
                     /locus_tag="VGA92_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9845246.1"
                     /translation="MVLAALGLLLIPLLSEANAASNPNLYVSAENPFYDNHFAGSMVI
                     EVVVSDPNLSDTDEGKGEPDVTLNGNALRMVQAGDGKWYAYFANVDKAKIADQISLDA
                     GVAGEGLDFGVFCGRDTISLGPSFSETDGIALPKDTMTGSTNGNSGFNTCTGAPSGSI
                     INNVVRNPRSINTNSAVPTGQIGLDDDAWPIIQLYSFSSNVVIQYNAAGGTQQVTLQY
                     DDIPNISLSLDRADYPPGSEVFVTIHDIQLNQDPTARDSWTFNVGSPQATFYAAFTEN
                     GANAANGAAGLTNLVSKLASLGFDNNGKISMNLGSVADLKENGFQSDEATDGGPNTYT
                     QIVTFVESQPNTGIFENFDFGNEANIMVLSTTPRGQAATIEYNSKSASIVSGQGTASV
                     NLGTTQVLSGQKIPVTVTDSDQNINSGARDRLEVFRSSAIIPSLTIGSPITLEDADDV
                     QIYNPAAEPPVGGVSVSSSVNDTNSDRLILDTRPSTGIANQNFEQISIDLDLTATDLQ
                     SLLIDVSVANNFGTNWINYDFRSLEKQLGITDFSDTSMSLYFGLGDLTPITIVDVGDM
                     TGAQGFIQFDDADVDLISSESGSVFLVINFDESANSAPQGAISTETDTQPIVFDLFSF
                     GTKNNVDVNNAIYRFELQETSTNTGIFTGTLEYLIANQLNQYDASTIRTLRIIDEDVK
                     FFVNQRLIDEKGINIAYSDVSQVGSTEISSSKTDINTHSGVVSLTAKAFRFGQPVTVV
                     LNDPDLNVKHDGIDIYSVIDDPASANVDTVGTTGGGILLEVLIKDIRYKRCTINGTEH
                     GGLAASGFSLVETGPNTGRFQGVFKLPSQICNKDGTKLISSAGGIIDLKYHDFRDSSG
                     QQNIFSLSKGATSTPTSPPVSNAAKPELKTPAIPSWIKSNAERWSSGVISDTEFIDAI
                     EFLAKEKIIAVKKNQSLEQSKSVPDWIKKTASWWADSLISDEDFAKGLEYLVNTGVIR
                     L"
     gene            11233..12402
                     /locus_tag="VGA92_02145"
     CDS             11233..12402
                     /locus_tag="VGA92_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9845247.1"
                     /translation="MTRNLKFRLYPNHSQWKRLQNTLGACKWTYNKMVEKINNEGFQT
                     RNDLNYFLTELKEQESWLYSYHSKMLQMISTQIDGAQKSLIALNKKGHKVGKLKFAKY
                     IEYRTFAYNQSGFEIKDGFLHLSKIGKIKIMQHRKIPENIEIKQIAVTRSKTGKWYCS
                     ITCDVDAVLTKFSITKSVGIDVGIKNFAYDSDGFVTPNPLNLQKMLKPLARAQRKIAR
                     RQKGSNNRKKAVRWYQIVHERVKNRRKDFLHQISTQYARKYDVIFLERLQKLNMVKNH
                     KWARNILDSGWGTFTNMLDYKCMLVEVPAKNTTIDCSRCGNAVPKSLVVRTHRCDVCG
                     LVLDRDHNAAINILKKGLDIFNINLPQELREVTPVEIIPWSVKQEEATGRLVVVH"
     gene            12426..13784
                     /locus_tag="VGA92_02150"
     CDS             12426..13784
                     /locus_tag="VGA92_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9845248.1"
                     /translation="MQNVFLQRTNALKISLIAILSAFVVEFVFGIISNSLALLTDSIH
                     ALLDSVVTVVLLMAARFAIKPPDAEHTYGHGKIESLGGLIGGIAIFLIAIFFIYEAII
                     RIQSPPPTIVPGMLALAAAIYTIGTDVFRITLLKKAINKVGGTTLKADFYHAFMDLGS
                     TLVVIVGIILVTVGFHDGDFVAALILGVLLCFLSLKLVYKTAQELTDVISPTLVSRVR
                     EIVLSTDEVLDAGPILMRKSGDLIFADVTISLRADASFDRAHEISSRVEENIKKSISN
                     SEITVHFEPSWRDVPRDSKIYEVASNVPGVKGVHNVSQHTSDSGIFASLHVMVDRQMS
                     LEEAHKVSEIIESKIKQSLPEIENITIHLEPYIAIPKDLKGHKTSDEKIIQLLKGHPE
                     VKRVGRIVTLNFDELLKIDINCSFDKNLSIEKVHDLTSQIEQNIRNYFKNSVITIHPE
                     PY"
     gene            complement(13781..14959)
                     /locus_tag="VGA92_02155"
     CDS             complement(13781..14959)
                     /locus_tag="VGA92_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation transporter"
                     /protein_id="HEX9845249.1"
                     /translation="MELSNKSSKGKMIASGIIPFALLILLVAYIFGPGSELLDLGVAL
                     PEVTIEKVDFVDHEIRATVRNTGPIPVEVTMADINDRIQPAAVEPDRFLERYETALVR
                     IPFEWNEAQPYIIGITIEDGTRFEKEIEAAATALEPSLELAGFFAIIGTYVGIIPVMI
                     GLLWLPFIKKISKSKYHFFLALTAGLLLFLGIDSVEEALEVSRESLASSFNGALLVAT
                     VIVLSFFGLYYTGEKLVKRTESSRMTKPVAIALMIAIGIGLHNFGEGLAIGAAVGLGS
                     IAFSTFLIVGFALHNTTEGIAIAAPMSRGKTMIGKLAGLGMIAGAPAIFGAWIGGFVY
                     SPFTSVVFLSIGAGAIFQVTITILRWMREEGDKNLSSAAVASGFAIGMLIMYLTSILV
                     "
     gene            complement(14949..15986)
                     /locus_tag="VGA92_02160"
     CDS             complement(14949..15986)
                     /locus_tag="VGA92_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9845250.1"
                     /translation="MTSILVVTTILATLYFVYPNISDAKNEEKTFVTHTGAVIKTSGE
                     VLDPVYTAQTVEFDPEKYLREFNYGRISKLEDGRTLREFTIIAEDDKIMEISPGVFYN
                     VWTFNGTVPGPTIRATEGDLIRIYFINNGSKEHTMHFHGIHRAEMDGVFEPVGSGGGR
                     FPYEFEAGPVGVHPYHCHVMPLEEHIVHGLYGVYIVDPKEGRPAADEMVMVLNGFDTD
                     FDTENNFYAANTIPFYYQHHPIQINTNELIRVYVVNMVEFDPINNLHLHGNLYYYYPT
                     GTDTVPSTYTDMITLSQTERGIMEFEYTYPGKYLFHAHKVEFSEKGWVGIFLVKDNPD
                     QKSQGEIGYGT"
     gene            16151..16651
                     /locus_tag="VGA92_02165"
     CDS             16151..16651
                     /locus_tag="VGA92_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845251.1"
                     /translation="MRKSHLILFIGGALVAIGMVTSFYGSNLVTQDLAIVEGVASDAS
                     PVELIKELDPSIANTGVFVVQAESFDGLVATILDPIGQQITSIEISQRSTEEQFEITV
                     KGSYKLLLQSSATSEVPIVMGLTHMPDKNFIALNLLGQSLIVSGFVGVGLAVILEIKN
                     RKKKIS"
     gene            complement(16644..16949)
                     /locus_tag="VGA92_02170"
     CDS             complement(16644..16949)
                     /locus_tag="VGA92_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845252.1"
                     /translation="MTENYDKGKVLEDLFNPDISIILAELENGSKESAYLANKIGISE
                     EEIKTRLGYLIGAGFVISSNSPLSYTVNADKIAKIMESDENYKNVVDGLTELDSYLN"
     gene            complement(16957..18300)
                     /locus_tag="VGA92_02175"
     CDS             complement(16957..18300)
                     /locus_tag="VGA92_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9845253.1"
                     /translation="MSLHNFKNFVYFSATQEKRQDSNEKSRTLDQKTLERILKTLASQ
                     SMREKAPTLDELENILLTNIRELSSDSVPHEQSDQIPKSDDIHHITKYLIEQGYLKDD
                     TNWLTKKGFFAIGNQILRDLMNELKTGEFGLHETKSVGIGTTILDTTKKFELGDELKL
                     LSVPITILNTISRLTKKGSELKFPLDIDVDDFEEFETIEDVRTAVVYCIDLSSTMKSS
                     LTGPSISRIEAAKRALWSLYVLNNKFFPNDSISVVGFASMASIVDPFDIPFLRTYDAN
                     DDFLHYTNYQAAFRLAKKILQKNSAKNKRIVMITDGQPSACFVDTENQKNSILSEKPY
                     SNFYEPDPSLLSKIREERNMKIDSGSMVYLCYRYKKVDPKVNEQTINEAKKCKRENIQ
                     IDTIVVSEEAELLDYVKQMEKQLQGKTYHINQNNMEKVLVIDYLYNTKKILSVKN"
     gene            complement(18287..19915)
                     /locus_tag="VGA92_02180"
     CDS             complement(18287..19915)
                     /locus_tag="VGA92_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845254.1"
                     /translation="MTAVSELVQRCYSSEPKYTEIKAGVPEDYEDIKTIRDLLEINYK
                     LLEAREQLRKNLISAMKSGEKKYPGILGYDEDVIPALDRAILSGHDIFLIGQIGQAKT
                     KLVETIAKNLLSPIPIVDGSITSDCPMDLPADELISLLEGQEANKSSPKFYISPESSQ
                     KILDNNLDTKITWVDGQQRFRYVLATPDISVKDLVGYIDAIKVAKKGVEMFKIESYSP
                     GQLMQAKHGIFCIDELPVLDPRKQVALLSVLQEGRYTTGSYPVMFEPKTVFFATANPI
                     DYTHSGKIIEPLYDRLKSHVHTHYPKTMDDEMLIILQEAKTANCFIPVFVLRTLTRLV
                     QKARNNPEINQAKGVSVRFGIHGLELLVGESERTRAIPYQTVAVPRHSDMYCLAQIAK
                     FELSEIDDTLENRHHIFGELLGSAIKDTCLEYLQGIDAEILESIKQEFTGKTFQVSQK
                     IVWSNGQTSYEKQLQNFTQLNNLIISKLEQIKQEQSSFVEKLAADKIPNKNITLGTQA
                     VNELKAAVLEVVLDGLCFVTPKIIDKKEAGYVAA"
     gene            20016..20669
                     /locus_tag="VGA92_02185"
     CDS             20016..20669
                     /locus_tag="VGA92_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9845255.1"
                     /translation="MSAQEYRHIVRIVGKDVPGDKKLSVGLAQIKGVGYNFANALIGA
                     LDLNPNSNIGFLTESQVEVIEKALKDPTSVNFPAWIYNRRKDVESGNSFHLITSDVAF
                     TVRNDIEREKQVNSWRGFRHTFGLKVRGQRTRTTGRKGASVGVKKGGKVLPAGVLPPG
                     AEAGAAPAAAGAAPAAGAAPAATAPAKAAAAGKSPAAGKTPADTKAKAPAPDAKAKK"
     gene            20679..21224
                     /locus_tag="VGA92_02190"
     CDS             20679..21224
                     /locus_tag="VGA92_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9845256.1"
                     /translation="MGDPHYPSRIWRKPKRPLNYDFMMEDLNTLGTYGLKNKRELWKA
                     RTELSRVRHQARSLLALRQEIREQKEPILMNSLTRIGLVKEGATLDDVLNLDIDDLLS
                     RRFQTMVQKKFSFKTPYQARQAIVHGHVTIGDRVVTIPSYIVNIKEEGLIQLSPKFKV
                     QNNPPAEAKQEESEPQSESQS"
     gene            21271..23031
                     /gene="glmS"
                     /locus_tag="VGA92_02195"
     CDS             21271..23031
                     /gene="glmS"
                     /locus_tag="VGA92_02195"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9845257.1"
                     /translation="MCSIIGYYGDGLAAPILVKGLKRMEYRGYDSVGVATKSNQEILV
                     KKGVGKVAEVNSSLHLDSLPGNIGIGHTRWATHGNVTDVNAHPHPSSSGQIAIVHNGI
                     IENYDELKVDLEKKGYQFKSQTDSEVIANLLQFNYDQTSDIKKAMIKTILGLKGHYAF
                     VAMFQNGTLSAARHHEPLIIGIGKHGYFLSSDVLGFIEKTDDAIYLDNGEFVLLDKIG
                     IQIYNFEGEPVKHQVTKVSKEFADAYKGDYAHFTLKEISEQPETIIQAGESSMDAINA
                     ATDFIKHAKNIYITGSGTSYNAALVAKYLWQKHAKIKVEPIISSELSFSPDAIEPNSI
                     LVAISQSGESADVLEAVNIAKKSNAKILSLVNILTSSLAQESALVMGMSCGPEIGVAA
                     TKSFTSQLSVIYKITEKLCDGCIKIDNAKISDAVGVVLSDHSKIKQIAKDLRDVTDIY
                     ILGRAVHYPIAAESALKLKELAYIHAEGIPGGELKHGPLALMDSSVYVIIINPNDFTY
                     NDTLTSAREIKARGAKIIGISDKPSDVYDYWISIPTIDEAFYPLIEIIPIQLLAYYSA
                     IEKNTDPDYPRNLAKSVTVK"
     gene            complement(23018..24082)
                     /locus_tag="VGA92_02200"
     CDS             complement(23018..24082)
                     /locus_tag="VGA92_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HEX9845258.1"
                     /translation="MNQDIDILVDQQKGREFLESVEKKHFLFSLVISYTDTCTIPGIT
                     VAGKNPALLQYTSPADAEFLNFGYCKCINVIPMTPDGKPTPALLTKTALESASISNIV
                     INAGSKILPKLPYFETGLPFGKNIATGPAMPLDAVTHAVEYGRMIGRSLGSITDCLVL
                     GESIPGGTTTAQAVLASLGIDAKVSSSMQDNPLALKNEIANTASKRIDSKNPFDVVAN
                     AGDPMIPTIAGILSTASSSTKVILAGGTQMAAVLAFSKITGYDKDNTAIATTSYVIDD
                     NTANLVDTIKQIDQIPVFSVRLALKDSKINGLRSFADGFVKEGVGAGGASLAAMLKVG
                     IDSKHLLQLTEQQYENTILL"
     gene            complement(24083..24667)
                     /locus_tag="VGA92_02205"
     CDS             complement(24083..24667)
                     /locus_tag="VGA92_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase family protein"
                     /protein_id="HEX9845259.1"
                     /translation="MQNFTYVVEDENAGDAIIIDPSWDLDDLKRVIEKNNLKVQYIVN
                     THYHFDHTLGNETMAKETDAKIIQYKTSTLKHDIAVSDGDKIKFGSSELSVLHTPGHS
                     KDSMCLVGDGKIFSGDTLFVGTCGRVDLPGGDAKELYHSLVDVLRTLDDDLIMYPGHN
                     YGSTPTATLGNQKKTNFVMQPRTEQEFLQIMGIG"
     gene            complement(24694..25341)
                     /locus_tag="VGA92_02210"
     CDS             complement(24694..25341)
                     /locus_tag="VGA92_02210"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX9845260.1"
                     /translation="MEITVKQMYQIEENGHQMGFLRQLMMENAGAAMVKQLVNKFPDI
                     SSKKVLVFAGLGNNGGDALVVSRHLAGFGCKVIVVLLGNPKGIKTEECRTNWQILEKM
                     KSVQLGFGPDDASNFNVDIIVDGILGTGITGTIREPQASAIDLINKSSAFKISVDVPT
                     GMNPDTGQTADKYVKADMTVTFHKMKVGMPKRIDICGTIFVEKIGIPPEAEIGVL"
     gene            25415..26026
                     /locus_tag="VGA92_02215"
     CDS             25415..26026
                     /locus_tag="VGA92_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX9845261.1"
                     /translation="MLDSTKRSLEKISKSLEKNLEGRESLIKNTRQIVILCSESIIDC
                     HKNDTKSAAKKVLAAEKLLAQYRKKTAEGLYRYLLLPEQEFVEASSLLSILQGNQVPA
                     IESLHVKEESYVLGLLDCIGELRRNVYDKIRTGHAAEAKKMFDIMEELYLQLYPFAYF
                     DKIVKEVRRKLDVARILVEETRIAITEEIRRSELIKNMKKSRK"
     gene            complement(26220..26417)
                     /locus_tag="VGA92_02220"
     CDS             complement(26220..26417)
                     /locus_tag="VGA92_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845262.1"
                     /translation="MWKIASVDRTISKKLEKYKANKQLTDNYFKFIEDLTNADDPRKM
                     GEPKQGRFRKCFGKHRQNHTV"
     gene            complement(26421..26570)
                     /locus_tag="VGA92_02225"
     CDS             complement(26421..26570)
                     /locus_tag="VGA92_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845263.1"
                     /translation="MQKSHKEAKSELNPELDDLLERVVSGKEKVTVYDTPEDYLKHAK
                     KIIKE"
     gene            complement(27054..27138)
                     /locus_tag="VGA92_02230"
     tRNA            complement(27054..27138)
                     /locus_tag="VGA92_02230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27100..27102),aa:Leu,seq:gag)
     gene            27188..27424
                     /locus_tag="VGA92_02235"
     CDS             27188..27424
                     /locus_tag="VGA92_02235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HEX9845264.1"
                     /translation="MLIPVRCFTCGNLIADRYEEYQNRIKAGEEPAKVLDSLGFKRYC
                     CRRMLLTTVETIQQIIPFYEAIQRRYLEIQSELE"
     gene            27426..28664
                     /locus_tag="VGA92_02240"
     CDS             27426..28664
                     /locus_tag="VGA92_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX9845265.1"
                     /translation="MPKISSIRCRLVYNSRGGKTIEVDVISDGKHLGRACVPSGASVG
                     KHEAQSFANNKPESSLQILKKNLRKFVGLDSNDLKSIQKVLRQVDNTPNYSKIGGAAA
                     YAITIASVDSAAKALGVPMFKLISQTKKWRFPFPLGNILGGGAHAGPGTPDIQEILIC
                     AVGAKSVREAIETNFAVHKELGKILRSEDPRFTNGRGDEGGWAPELDNERALEVSARA
                     CEKLGYTLGKEVALGVDFASSTQWDEKKKKYLYERAGFENTPDEQIDFAANIIKKYKL
                     VYAEDAVHEEAFDQMARLAKKFPKTLIVGDDLTVTNAKILKKAIAKKSCNAAILKVNQ
                     AGSLYDALEFAQVATKHNIRLITSHRSGESTDYQISHIGIATGSKMLKVGVVGGERVA
                     KLNELIRLSEYNLIHGMAEI"
     gene            28672..29295
                     /gene="rpsB"
                     /locus_tag="VGA92_02245"
     CDS             28672..29295
                     /gene="rpsB"
                     /locus_tag="VGA92_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9845266.1"
                     /translation="MSQQAEAQDIKKKILSTGIRVGTTVKTTFMQPFITKASPEGLYM
                     IDLDKTLARIKTAAKFANKIEFDKILVCSGREYAFTPIEKFCELTGAKMMLGRFMPGT
                     LTNPSLSYYTEPKLVIISDPQIDSQAITEATNAGIPVIGVSNTDNVTSKIDLVIPANN
                     RGRKSLATVFWLLAREILIQKGQLSESDPMKYEIDDFETKITEEEME"
     gene            29295..30125
                     /locus_tag="VGA92_02250"
     CDS             29295..30125
                     /locus_tag="VGA92_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HEX9845267.1"
                     /translation="MRIRTPAVAGMFYPKEKNELRESIQSCISHKYGVGQSKTEQLKV
                     YGAICPHAGYMYSGPVASHSFQYLSNQDFELAIILGPNHWGVGCSIATLKDCTWQTPL
                     GDVDVDSEAAVEVNKNSKLIEIDFFSHTRDHSIEVQVPMLQEFYKKTFKILPIILNDQ
                     DYEFALEVGAAIAKLAAKKKSIIIGSSDFTHYEENEFAHSQDKALIEPILDLDVEKFY
                     KVLNERRVTACGYGAIASTMVACKELGATQGKLLQYATSGDIAGDKSSVVGYASIIFT
                     "
     gene            30122..31084
                     /gene="mvk"
                     /locus_tag="VGA92_02255"
     CDS             30122..31084
                     /gene="mvk"
                     /locus_tag="VGA92_02255"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEX9845268.1"
                     /translation="MRSIASAPGKVILFGEHFIVYGGKAILCSINKRITIESQLSNSD
                     KIEINSGLGTISVSKNEPLQNIDPVFKPVIYVAQKILQKFNSDSGLKISITSEIPAGV
                     GLGSSSACCVAAASSISGLFSNYSKEEVLQLAIEAEKTIFENVSGADSTVCTYGGIIE
                     YSRDGKIKKLDFVPKFQLVVADSKIMHSTSQVVSKVKKFKDDNPQVFSMLCDKEASLI
                     EETLGALKKNDLNTIGAKMNQNQAHLEKIGVSNDLLGSMLGTVNNVAYGAKMTGAGDG
                     GCIIALVNDTNLDRVIGALRSKNYECFAAQIDTTGVEQKVKDSP"
     gene            31129..31869
                     /locus_tag="VGA92_02260"
     CDS             31129..31869
                     /locus_tag="VGA92_02260"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HEX9845269.1"
                     /translation="MILIKLGGSIITNKERPLSPRISTVDKIMRQLKKIQEPFIVVHG
                     GGSFGHYWSVKYDMHTKPAKYDLHGVSIVKNSMIELNKIILDRFVKNRLNPYCLPPTD
                     FMDGDKPIPSKVKEIKKIAESGLIPVTYGDALWYGEKKSYILSGDRIMSILAKSLRPR
                     LVVFVLNVDGLYSDLKTKKLVYEMKDHQVSIQKISMDVTGGMRRKVQEATKISKMGLK
                     VFFANGNKPERITNAIQKNKFEGTIFRG"
     gene            31873..32514
                     /gene="idi"
                     /locus_tag="VGA92_02265"
     CDS             31873..32514
                     /gene="idi"
                     /locus_tag="VGA92_02265"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX9845270.1"
                     /translation="MADEHLILVDSDDNPIGFEEKVKCHLPNGKLHRAFTVLLFNKEG
                     KLLLTRRSQSKMLWPNDWDGTVASHPRKSETYVSSAERRLPEEIGAACKLDYLFKFEY
                     HVPYKDVGSENEICGTLIGQVNEPFELKLVRDEISETKWVNGDDLVSDIEKNPQSYCP
                     WMLVALYLLPESDKKMIEKHKHIFDKWLKTEFKNNLEKSLQYHFPTNNWRLLN"
     gene            32514..33491
                     /locus_tag="VGA92_02270"
     CDS             32514..33491
                     /locus_tag="VGA92_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9845271.1"
                     /translation="MTSTLPKNTKLVNKYLGAKLDGSPAELYRASAHLIVNGGKRLRP
                     YLVIKSCQMLGGSTRDAIPAAAAIEMIHNFTLVHDDIMDNDEMRHGVPTVHTKFGMPL
                     AILAGDVLFSKAFETVSIDNVPNNKTSAKLVARLAKACVNVCEGQVLDIQMAEGKKIP
                     SKSDYIKMIEKKTSALFEVSCAMGAICANTNGRDIANLASFGKNLGVAFQITDDFIGV
                     LGDPKVTKKPVGNDLREGKKSLPILLAIQKADSKKKRIILRVFGNPKATKKEINDAVQ
                     AMRSLEIEKDVRKVALQYADKAKRSLSAYSGSAKAEIISLLDFVVKRSL"
     gene            33495..35195
                     /locus_tag="VGA92_02275"
     CDS             33495..35195
                     /locus_tag="VGA92_02275"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9845272.1"
                     /translation="MDDVVKSKIRKLALQNAAEHGKTQDKIVLAKILATEPQLRSQVK
                     EIMPAILEIVSSVNQMSQEQQRKEIEKEFPDLLAEKPKKEERDGLPPLEGAEHGKVVT
                     RFPPEPNGYPHIGHAKAAIIDEEYAKMYGGKLVLRFDDTNPEAERLEFYAAIKVGLDW
                     LEIKFDKIKNTSDDIELLYKKCLEMLNGNFAYVCTCKQETISANRREMKSCKCSLGAL
                     EQNLERWDKMFSKYKQGEAIVRFRGDMKSENTVMRDPVMFRIIETKHPLLSDKYRVWP
                     SYDFAVAIEDSIDGITHAFRTKEYELRNELYYAILDALKMRKPQVLEFSRLGFEGMPV
                     SKRVLRPLIEEGKVSGYDDPRLPTLEALRRRGIRAEAIRKFVLSLGFTKADTLAPFET
                     LESFNRKIIDSDSIRLHMVKNPASIKVRGLPKTVIMQNHPTKDMGSRTIELDENFYVE
                     QDDLKNAKEGDQLRLIGLGNVRLTKQQPHEAEYVGDDLTPNIAKIHWVVQKTAQKIKI
                     LIPSQLFFGEQFNEKSLEEINVFSEPYYLELKEGKEVQFVRFGYCRKDSMLQAIYTHK
                     "
     gene            35202..36062
                     /locus_tag="VGA92_02280"
     CDS             35202..36062
                     /locus_tag="VGA92_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9845273.1"
                     /translation="MKIGRFLIAGRETYGFVKDGRIATKDEIITKTGIPLPLNIKEFL
                     FDGWYEEIISHKPDLEYGVSLDDAKILAPIPNPPKIICLAFNYVDHAEEQNLTPPTEP
                     AIVLIPRTTLNGTESEIVCPSFVKQLDYEVELALVIGKNCKNISEKDAMSTIFGYMVF
                     NDVSARDVQEQDKQFGRAKGFDTFAPCGPWITTRDEISDPQNLKLITKINGHLRQNSS
                     TSNMFIKIPSIISKLSQVMTLEKGDVISTGTPAGVILNKPNATFLKDGDKIEMEIEGL
                     GKLENTIRFI"
     gene            36097..36405
                     /locus_tag="VGA92_02285"
     CDS             36097..36405
                     /locus_tag="VGA92_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX9845274.1"
                     /translation="MGKIIVGKVSDIPAGKMQKVTVDGKEILVANVDGSFYACDDTCT
                     HAGASLAEGTLEGGIITCGWHGAKFNCSSGKLDKFPAKISDLKSYKTLVEADNVFVEV
                     "
     gene            36452..36724
                     /locus_tag="VGA92_02290"
     CDS             36452..36724
                     /locus_tag="VGA92_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HEX9845275.1"
                     /translation="MSDKKAQNQKSFDLALQTLTQIASNPTTPKNIKKTISDLITNLK
                     SSQDPMPIRVANAISQLDDITQDPNMPSYVRVTLWQAVSTLESIRE"
     gene            36775..37536
                     /locus_tag="VGA92_02295"
     CDS             36775..37536
                     /locus_tag="VGA92_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9845276.1"
                     /translation="MKIEEYLASLPPSIISGEDVQLSDDSVREVFKFVKLGKNDVFYH
                     LGCGSGNSLVIALEEFFVNKAVGIDKDDTKIAKAKKLTKEKNLQNAELRCQDILDSDL
                     SDATVILFWFSDTKTVEKMFERFSKLKPGTKIVTIFDPLPRILPEMVSFPYIVHQVPF
                     RPARDLREQLVAIFETECIDFTTAWEFAERYTKAVGSPDSGNDRFLTILQTLMIWINA
                     RNLGLSCTQEIPAPVKSYMEILQNFFNIEVKHLLK"
     gene            37580..37927
                     /locus_tag="VGA92_02300"
     CDS             37580..37927
                     /locus_tag="VGA92_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845277.1"
                     /translation="MNGQISINVSATDYDKSSKEITNTLRLVEEMVHETDGFVVTDSE
                     FAFGWHFYVISVRTDLVQKLADQMGSDFQKLKGKTLDKKFLSWLGQKMEQKSIKVKFA
                     IKEEMESSKYGIF"
     gene            complement(37934..38884)
                     /locus_tag="VGA92_02305"
     CDS             complement(37934..38884)
                     /locus_tag="VGA92_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9845278.1"
                     /translation="MKALYFDGTKIALRDDYPEPKSGEALVKVKLAGICGTDLEMIRG
                     YMSYDGVLGHEFVGVVEKSDNPNLVGKRVVGEINAGCGKCNYCTTGLERHCPNRTVLG
                     ILKRDGAFAEYLVLPEKNLHVIPDSISDRQAVFVEPLAAAFEINEQLRIEPNQKIAII
                     GDGRLAQLIARVLRLNNSHITCFGRHQNKLQMLQKIGIDTKTQITSADEHQFDIVVEA
                     TGRESGFSDTMRLVKPRGVIVLKSTMASKNKLDFAPAIINEITIVGSRCGPFRPAINA
                     LASGLVLVDDLISAVYPISDYQKAFEEAAKSENLKILLEP"
     gene            complement(39022..39375)
                     /locus_tag="VGA92_02310"
     CDS             complement(39022..39375)
                     /locus_tag="VGA92_02310"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX9845279.1"
                     /translation="MGYLIKAINSLEIAKIAMQKEAYDNAVMNAVHSSINALDALATS
                     RKAKRASSQHIDVLILIKGILNPQEYKDVERQFKLLLGLKNASEYQPDLMSPKDAESS
                     IKLAERILAKVKAKM"
     gene            complement(39401..40009)
                     /locus_tag="VGA92_02315"
     CDS             complement(39401..40009)
                     /locus_tag="VGA92_02315"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9845280.1"
                     /translation="MKLHNYLEQILGNRIAVRVLRTLVRYKGKIYTIRRLAEDAGASH
                     PEVSKTLDELEKFGIVQIQPVGRAHQVSLNERSYVLGKIIKPALKAEENTLAEVISIL
                     KNRLGTKKIISAVIFGSVAKREERDDSDIDVLVISNDLDHAISAISDAALEISLKFQS
                     KVSHIVFSESQFRSKKKNNTFVRSILDNHILICGKELASVLK"
     gene            complement(40218..40289)
                     /locus_tag="VGA92_02320"
     tRNA            complement(40218..40289)
                     /locus_tag="VGA92_02320"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40254..40256),aa:His,seq:gtg)
     gene            complement(40500..41522)
                     /locus_tag="VGA92_02325"
     CDS             complement(40500..41522)
                     /locus_tag="VGA92_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX9845281.1"
                     /translation="MKKVIFTPRLPHLRRHPQADEVIQSHVETIQGNGFSWMDIQNPD
                     RAEIEKLGQIYNFNTLNLDDSISKIQLSKIDNYDDHSFIILHFPPLVLKRGMPKFSQL
                     AVFIGKDYLVTIHYGELKPLVDMFAHCKENAQLRGELLGKSSGFLLYKIIDALVDDLL
                     HTLRRIIANLDEIEDDVFDENKSTPKKISLLRREITILRRIVNPLRKIVLETSKNTQR
                     FSNPDDDLVIHFDDVIDHIDKVVETLEESKETMEIYKDTDFMLSTEKSNKILATLTII
                     FTLTIPPTIVGALYGMNVNLPGGIQTGSWDFFGPYTTLIIMMIFSFIPSLLMFLYFYN
                     KKWLVD"
     gene            41906..42934
                     /gene="twy1"
                     /locus_tag="VGA92_02330"
     CDS             41906..42934
                     /gene="twy1"
                     /locus_tag="VGA92_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-demethylwyosine synthase TYW1"
                     /protein_id="HEX9845282.1"
                     /translation="MSCSGEVIEPENELIQIRPNILKQLKKAKYGVADHATVELCHWT
                     KKSFKNGGDCYKHKFYGISTHQCMEFSPAGMHCENRCVYCWRPMEFYDSLKMDPRKVA
                     EPAEMMEKLMEERRNLIMGHYGDPKSDRQKLDESLLPSHYAISLSGEPTMYPKLPQLI
                     KYLKDLKTTKSIFLVTNGQEPEMLQRLQDENALPTQIYLSTNAADYESFIKINKPKYK
                     DSWERWNKSLELLSKLDTRTVLRITLIREYNDGEKIIPAFAEMLRRANVHFIEVKSYM
                     HIGRSTNRLERDNMLEFDEVRHFAFELARQSGIYSIMDESDASRIVVLQNQQRFTERW
                     IAAYLNTK"
     gene            complement(42941..43195)
                     /locus_tag="VGA92_02335"
     CDS             complement(42941..43195)
                     /locus_tag="VGA92_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845283.1"
                     /translation="MQLDALAIRVLSKMYHYGYVGGRHTSTESLKRSFASHEKGMVDK
                     AIKKLVKSGLITFHPTSYGHQYSLNPNRLKEIEAIIENQM"
     gene            43289..43642
                     /locus_tag="VGA92_02340"
     CDS             43289..43642
                     /locus_tag="VGA92_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845284.1"
                     /translation="MMKQKTKRAALSEKKSYEIGPLTSVFPCSESKILDHMVTMRGLD
                     YSISDISRISGVNFKTTLNIIHKLEGQEVIKKTRTVGKAIMYILNENSSQAKSIIKLA
                     FEIAKKRNNEILAKR"
     gene            complement(43639..44193)
                     /gene="rdgB"
                     /locus_tag="VGA92_02345"
     CDS             complement(43639..44193)
                     /gene="rdgB"
                     /locus_tag="VGA92_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX9845285.1"
                     /translation="MRKSYDLVFASSNKNKYREAKEILSQFDIRLGFLKFSAVEIQSH
                     SIGQIAKQKALDANKKCRSAVIVEDDGLFIDSLGGFPGPYSSYVFKTIGNAGIINLVK
                     TKKNADFVSVIAFCDNRRRPMLFEGRIRGRISAKPKGKGWGYDPIFVPNGKNKTYAEM
                     SDKNTISHRYRALQKFTTWYSKIH"
     gene            complement(44177..>44727)
                     /locus_tag="VGA92_02350"
     CDS             complement(44177..>44727)
                     /locus_tag="VGA92_02350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HEX9845286.1"
                     /translation="KTRKKKPYRNTILDDKIRRQRTIREASLLAESKLFGVRTPLVYQ
                     VNANDCTILMQHIKGDLARNLKGSKLINACKEIGKITGTLHKNGIMHGDLTTSNFVVS
                     KNKTYIFDFGLAQKTSKVEDHAVDLRLFKEILNSAHVDMMNVFWPSFLAGYKSVVETE
                     RFNKVLNYVSVIEGRGRYAQVV"
BASE COUNT        13367 a         8950 c         9401 g        13009 t
ORIGIN      
        1 tgttcccccc gacgttagca atatctcgct tggttgtgcg tttatcagct cagcaattct
       61 ttttcttgca gtctcgattg cctttgtggc tatccttcca aacctgtgaa tggaagaagg
      121 attgccatag ttttctttga aatatggcat catctcctgt aaaacatcgt ctcttacagg
      181 cgttgatgct gcattatcca gatagatcaa tctgcaatca cgttgatttt gcccggtctg
      241 tatccgtcct cgtcgactgt cacagaatca aatccgatta gttttagctt tgcaataagc
      301 tcatcaagtt ttgtcttttc tgcaaaaaga gacaactcgt ctctgccaag ctcgattttt
      361 gcagatcctc caatgtcacg tacacgaacc tgttttgctc caagaatttg ttttacaaag
      421 atttccccta cctcaattct ggccaatttt tctgccgtta cacgctgacc ccaaggtatg
      481 cgtgaggcaa gacaggaatt ggatggccta tcaaatactg acagcccaac tagttttgct
      541 attctacgaa tctcttgctt tgagagcccg acttgcacca aaggactttg aattccattg
      601 ttttttagag cctgaattcc aggacggtaa tcacctagat cgtcaatgtt tgtgccatct
      661 actactagct ggacgttttc tttttgagcg atttctttga gatgttctga tagctcagtt
      721 ctacaatgaa agcacctttc tttgtcgttt tttgtaaaat cctcgttttc tagttcatca
      781 tatgagatta tcaggtgtct aatcccaatt tcttggcaga tcttttttgc agactcgagc
      841 tcttcttgtg caagtgtttt gtagtctgca gtaactgcaa gtgctgcact gccaagtttg
      901 ccatgagctg catatgcaac aagcgcacta tccactccac cagagagtgc cacaatgaca
      961 ccttgtttgt ccgaaaacca atcctttagt tgctcgattt tgttcatttt agttgtcttt
     1021 ttacttgttc tttgattaat tcttcggttt gtttgaatga gagtgctagt ttttcagata
     1081 ttttcttgat atcgtcatat tctactttga attgctttcg attagaacct gactttgcta
     1141 ctttgcatcg tacagtaaag ttttggttac gagcctgtat tttcattctg agtatttgtc
     1201 ttggtacgat gagtcgatca gatgtcctga ttcttacacc caaggttcca gtctctgaga
     1261 ccagcacgtc aagtaactga ttgacatgat tcgaatcaca gattactgaa atgatgtgac
     1321 ccggtctacc tttttttgtg atagtaggga tgacagagac gtcttttgca ccccgtgaga
     1381 ttactttgtc tattacatgt gctaagacct cacctgaaac atcatcaacg ttcgtttcaa
     1441 gaatggcgac agaatccgat tctaggccac ttgttgattg tccttggact attttgagca
     1501 cgtttgagaa gccttcaaag tttttttggc ctgccccgta tccaatggag ctgactttca
     1561 tcattggata aaactctaca catttttctg ccagatttac caagatgctt gcgccagtag
     1621 gagttgtgag ctcgtctcgt actttgcttc cgtgtatcag tatgcctgat ttttcaaata
     1681 taccaagtat tgctccagct ggatttgata ctgtcccatg tgaaaagcta acagttccac
     1741 ttcccactgc aactggcaac gtaatgatct catcatcaaa atggccaagg tcatcaagtg
     1801 caattgcggt ccccacaata tctattacag tatcaatgct tgatgcttcg tgaaaatgga
     1861 cagaatcttt tggaacacca tgaatttttg attctgctgc aatgagtgta tttatgctgt
     1921 ttattgcaaa tctacttgcc tgctctgaga ggcctagttt ttgtgcggtt ttgccaatgc
     1981 agttttgaat ttctatgcct tttcgtttgt gattgtgttc atctaattga agaactaggg
     2041 atgttgcttt gattccgtgc ttgttgactc gtctaaaatc aattttttta atttttgatc
     2101 cgtgcagata actttgcgaa gtgttgacac cgtcaataat tttggctttg tttgcaccca
     2161 aatgtactag agatgataga atcatgtctc cagagactcc agctatttgt ggatcaatta
     2221 ctaaaaccat gagaattcaa gttcaaaaat gcatataaat tctcatgtca tggcgtgccg
     2281 agatcaaaaa gacagtcttt ttacaataaa tttaattatt gaacataaag gtgccataat
     2341 atgattacaa taatcggctc tggcaaagtt ggtggtgatg ccgcactatt ttcagcttta
     2401 aaaaaaatag acaccgagat tttgttgctc gatgttgtaa aggggcttcc acaaggcgag
     2461 gcaatggatc taaaccacat gttatcagaa caaggaattg atgtttccat cagaggttca
     2521 aacgactatt ctgacatgaa gggttcagac gttgtagtgg tagtagcagg ctctggaaga
     2581 aagcccggca tgacaagaat ggacctgctc aaaattaacg ccagcattgt aaaggatgtt
     2641 gtagaaaaca tcaaaaaaca tgccaaagac tccataatcg ttcccgttac aaatccgctt
     2701 gacccaatgg tacacatcac ttacaaaaca tctgggtttg agagaagcag aatcataggt
     2761 atgggaaaca tgcttgatct gtccagattc attcagttta ttcacgaggc gacaggttat
     2821 tcgcgcgatt cgattcgcgc cctagtaatt ggagagcatg gggaaaacat gctgccactg
     2881 ccaagatttt caactgtggc aggaattcca ctggcaaata ttttgccaaa ggacaaactt
     2941 gatcagctaa ttcaagacac aagaggagtt gctgccaaag taattgagct caagggcgcg
     3001 accgttcatg ccccaggaaa cgcaatatcc tcaatagttg aagcaatact cagggacaaa
     3061 aagaaggtaa ttccagttgc aacaccgctc gatggtgaat atggccattc tgatgtttcg
     3121 attggagttc cagcagtggt tggaaaagac ggaattgaaa aaataataga gctggatttg
     3181 aaccagcaag aaaaagaatg gttcaacaag ggaattgaca gtgtcaaggg cgccctttct
     3241 gggatctaga tcaatttttt aaacaaatca aacgcacaat cagcaatgga aatacttgat
     3301 gtgctagaat cattaaagag tggtaaaatt agcgtaacag aggcaaaaaa acttctttct
     3361 ttgtattcta ttgagaaaat cgaggatttt gcccaaattg atatgggaag acgataccga
     3421 aaaggagttc ccgaggtaat ttttgctgaa agaaagcagc ttgatgaaat aaaaaagata
     3481 attaccaaaa tactgggaaa gtcaaattct gttctagtct caagaataca aaaacaagat
     3541 tattccaaaa taatcgcatt tgctaaaaaa aacaagttca aaataaagga tggaaaaaac
     3601 acgacagctc tgttacttta caaaaaagaa caaaaaagat ctggaggaat ggttggaatc
     3661 gttactgctg gcacatccga tattggggtt gccgaagagg ccaggctcac ctgcgaggcc
     3721 atgaattgta cctgtatttg cagctatgat gttggaatag caggcttgca ccgaatattt
     3781 ccaatcataa aaaaattcgt gcagcagaac gtagatgtaa ttgtagttgc tgctggaatg
     3841 gagggcgcat tagcatctgt tgtggcgtct ttagtcgatg ttcccgtaat tggccttcca
     3901 acatctgtcg gatatggata tggtgaaaaa ggggttgccg cccttgcatc gatgcttcaa
     3961 agctgctcac ttggcctgtc agtagtaaat atagacaatg ggattggcgc aggggcagtt
     4021 gccgcaaaca ttgcaaacaa tgctaaacgt caataattcc aagattccca agctctgtac
     4081 caataccaaa tcccaccaat gcgattcccg taccaagtcc tgccatctca aggcctccct
     4141 tgagaatgtt tgtgttagct atctttgatt tgactgcacc aacaagaaat gatgtagata
     4201 ggcttattcc aatggcaatt atcaagggaa catatccatc taaaaagaaa aacggaatta
     4261 ttggtaatat gcctccaacc aaaaatgccc caaacatcct aaatgcactc tttagtggat
     4321 ttcctaagac ttcgacattt agctttaatt cttctgtgag cattgtgtca agccatactt
     4381 ttttgtccga agttattttg tttacaacca tttcaagttc ttttcccgaa aatccctttg
     4441 ccttgtagat atcttctatt tcttgtcgtt ccaagtctgg cttgttttca atttcccatt
     4501 cctctcttgc aatttctgat tttagtagtt cacgatgtgc ttttactgca agatagtttt
     4561 gaactgccat tgcttttgct cccgtaaaca ttccaacaag tgccgcaagt atgataaaat
     4621 tggatgacac gtctgctcct ccaacgcctg cagctatacc tagtgaggta ttgattccat
     4681 caccaaaacc aaaaacaaaa tccctaatcc cacttgattc ggaaagatgt ggttcggtgt
     4741 gtcttggctc agacatatct ttcatttgaa aaacctagtt aactgtgtaa tttaagcttg
     4801 aaaaatacgg ttctgtcaaa aatacactca gtaagtaatt tatagtatat ttcaagtcat
     4861 tacttagttg gctggcaaaa ggatagttct tactgcagac cgcagtctta tgactaatta
     4921 tcgcggtaat tttctttatg ggttcatagc gtgcggccca tacgagcttc tcccagaatg
     4981 ggtctttgac aaggtttttt gtccggcagt cgagacagac cctaatactg gtgaggccaa
     5041 ggtagcccaa gtaggcctgc gaagagtaga aagtgccttg ttgcagggat acaaaaggga
     5101 cgaagtcttt gtagcaaatc ctgattatct tgaaaaatca attggtcctg acaccaaagt
     5161 agtcggaatt aatgtaatgg acccgttagg aatggctccg gtgacaacta ccatgtcgcc
     5221 tgaaaaatta tcctatgtgg caatgaagtt caagaggatg tgcgcaaaaa taattcagct
     5281 aaagaaaaaa tataattttc acgtagtcgt tggcggaaac ggcgcttggg agctggccaa
     5341 aacagacaga atgagaatac acggactcga cactgtcgta gtcggcgaag cagacgagct
     5401 tgcactagac ttgttcaaag acttggaaaa tggggatgct ccagagctga tgcattgttt
     5461 tgtcaaaaac atacaaaaca taccaatcat tcatggtcca acagtcaatt ccttaattga
     5521 ggcaatgcga ggatgtggta ggggttgcga cttttgtgat gtaaacaaga gatccaaaaa
     5581 ggatcttcca ctagaaaggc tccagtacga ggcaaaaatc aacttggact atgggtttga
     5641 ttcaatttgg ttacactctg atgagatgtt actttatggg tgtgataaca aagactttca
     5701 gccaaaccgc gacgcaataa ctgagttgtg gcgaggactc aagtcacttg gcgcaaactt
     5761 tgttggaaca acccacatga cgttttctgc agttgccgca gaccctgagc ttctaaaaca
     5821 aatgtctgaa ataaacaaca tgcacacaac cgggagatgg cttgcaacaa accttggaat
     5881 tgaaactgtt gccccaagaa tggtaaaaaa acaccttggc gtaaagacaa aaccgttctc
     5941 aactgacgag tggggatggg ttgtaagaga gggtgcaaga gtcatgaatg agaatcactg
     6001 gttcccagca gccacgctaa ttatcggctg gccagacgaa acgcctgatg aaacacaata
     6061 caccatagac ttgattgaag actttagaaa gacaagcttt agaggcctag tcgcgccgtt
     6121 actataccag gactttagtg aaaagaactc gatgcacttt ggcaacctca acgaggcgca
     6181 gtttacacta ttttggagat gttgggaaaa caacctgcgt gtaattaacg acattgttcc
     6241 aatcatactt cgaaacaaga catacgggcc tccgatgaag gtcttcatgt atggcttgat
     6301 aaaggcagga acatgggcaa taatgcgata tttgcgcggc ctctgcaaag atctcttcaa
     6361 tggcaagctg ccagaagaca ttgttgaaaa atatgcaaga agcagatcta ttactgcgcc
     6421 ggcgtacact agatagaaat tatatttgtt ttattgcttc gtctgcaatt gtatttcttt
     6481 ttggagtcaa gacaataaag taggtcttgt ctgccctttc aattgcgctg acctttctgt
     6541 aagtgtttgt gttgatgtca aactctaaaa ttcttccatc aagtgtgcct ttggtgtaaa
     6601 tcataagata gacatcaccg ttgctatgag tcagcgggat aaacgcaaag acatatcctt
     6661 tgtacgagtt tattggcatt gcatttttca ttggaggcat tactgatgcc atgtacgaaa
     6721 agatatcctc aattgatgcg aattcctcgt attcaacgtg catatgattg gtgtgagaaa
     6781 tggggatatt aatcttagag gtgttttttg ctaattatgc ctctaagttt gactatggct
     6841 attgccggag caacaaagta cattccaatg tttagcaata tgatgccaat tccatatcca
     6901 agcatttctt gctcagagtc aatctcaaca tagttgagta tcgacagagt cgaaagcatt
     6961 ggtgtaattg ctgtctttac tacttctttg aatactggat tgtttcgctc ccagtctgca
     7021 atagttgggc taaatgagta gtagacagag ttaaagccag tcatgaagct tgttccagag
     7081 cttgtgccga ataacacgtt gtctcttact tctctgagta gttgtacttg tggtgcaagc
     7141 tcgctaccaa atgcggcagt tgcaatcaaa cagccgtttg ttggtggtgg aggtgtcact
     7201 tctggaacgc aaataccatc cttagctact gttcctgggc cgcattctgg tccggtatcc
     7261 ttaggtggct cgtcaatgca ttggcctcca actagcattt gtcctggtgg acagtctact
     7321 ggaggtgcaa caacacatag ccctgttacc gtgtctaaga cagttccagg cccacattgc
     7381 ggcgttgttt caaacggtac gcattgattg tttttgagaa tttcaccctc gccacatttt
     7441 ggtggtggag gaggtggtgg tacatcaacg tcagtacttc ccgtgccgcc attatagaag
     7501 aatgtagttt gtgctttttg agggccataa tttactataa tttgatagtc accctttttt
     7561 tgccaaagag gaccactcac aataaaagat ccttcaaagg agccatccaa ctgtggtttg
     7621 atttgcttta cttggacaac gttacttgaa gtgccattaa caagtggacc aataactcta
     7681 actgtaacat ccacgagatt gtttagatcc agatttttta caaagccgtt aaagactact
     7741 gaatccccct gatcgtacaa aggaagctcg gttttgactg aaagtggcgg aatctgactg
     7801 ccatcaactt gtccctgtac agtcggaaga gctgatgatg tgatcataag cgacaaaaac
     7861 actactgcca gtattccagc tcctttcatt tctaggcatg tacaacgttt tagggtattt
     7921 atgtattcta tgaaagaaaa gttgacttga ttatactctt cttgaacctt ttttgacttc
     7981 aaaacgtcaa ctcttttaaa attttgtttt tatttgttgt atatcgattt ttaattaaca
     8041 aaataaaccc caattgtagt ttatagccgt atgaccccag tattgacaag atattctagc
     8101 ccttttgcaa aatcttcatc tgagatcaga ctgtctgccc accacgatgc ggtttttttg
     8161 atccagtcag gtacagattt tgattgttca agggactggt ttttctttac tgcaattatc
     8221 ttttcttttg ccagaaattc tattgcatca ataaattcag tatcagaaat tacaccggat
     8281 gaccaccgct cggcattact tttaatccag cttggaatgg caggcgtctt taattctggt
     8341 tttgcggcat ttgagacagg aggactagtt ggtgtagatg ttgcaccttt acttagacta
     8401 aagatgtttt gctgtcctga agaatctcta aagtcatgat acttgagatc aattattccg
     8461 ccagccgaag aaataagttt ggtgccatcc ttgttgcaaa tttgtgaggg tagtttgaag
     8521 acaccttgga accgtccggt gtttggccct gtttcaacta ggctgaaccc agaagcggca
     8581 agacctccgt gctctgttcc atttatggta cagcgtttgt atcgaatgtc ttttatcaag
     8641 acctcaagca ggatgcctcc gcccgtagta cctactgtgt caacgtttgc cgatgccggg
     8701 tcgtcaatta cagagtaaat gtcaattccg tcgtgtttta cattaaggtc aggatcgttt
     8761 agaaccaccg ttactggctg gccaaacctg aatgcttttg cagtaaggga aaccacacct
     8821 gaatgcgtgt ttatgtcagt cttagatgat gagatttctg ttgagccaac ttgggataca
     8881 tcagaatatg ctatgttgat tcctttttcg tcaatcagtc tttggtttac aaagaatttg
     8941 acatcctcat caattattct caaagttctt atcgtgcttg catcatactg attgagctga
     9001 tttgcaatta gatattccag tgttccagta aagattccag tgttagtcga ggtttcttgc
     9061 agctcaaatc tgtatattgc attgtttaca tcgacattat tttttgttcc aaaggagaaa
     9121 agatcaaaga ctattggttg ggtgtctgtt tcagtagata tagctccttg tggtgcagaa
     9181 tttgctgact catcgaaatt gatgaccaaa aataccgatc cagattctga agatatcaag
     9241 tcgacgtcag catcatcaaa ctggatgaac ccttgtgcac ctgtcatatc acctacatca
     9301 acaattgtga ttggagttag atctcccaac ccaaaatata atgacattga tgtgtcagaa
     9361 aaatctgtga taccaagttg tttttcaagt gatctgaaat catagtttat ccagtttgtt
     9421 ccaaagttgt tagcaacaga gacgtcaatc aataagcttt gcaagtcagt agcggtaaga
     9481 tcaagatcaa ttgagatctg ctcaaagttt tggttggcaa taccagtaga tggcctcgta
     9541 tcaagtatta gtctgtcaga gtttgtatca ttaactgacg agctgacaga taccccacct
     9601 actggaggtt ctgctgcagg attgtagatt tggacgtcgt ctgcatcttc caaagtgatt
     9661 gggcttccaa ttgtaagaga gggaattatt gcagaacttc taaagacctc aagtctgtcc
     9721 cttgcccccg agtttatgtt ttgatcagaa tccgttactg ttactggaat tttttggcca
     9781 gaaagcacct gtgttgtgcc taaattgacg cttgcagttc cttggccgga cacaatcgag
     9841 gcggacttgg aattgtattc tattgttgcg gcttggcccc ttggtgtagt agacaaaacc
     9901 attatgtttg cctcattgcc aaagtcaaag ttctcaaata tgccagtgtt tggctgggac
     9961 tctacgaatg tcacaatctg ggtgtatgta ttgggaccac catcagtggc ctcatctgac
    10021 tgaaatccat tttcttttag atcagctact gacccaagat tcatcgagat ttttccgttg
    10081 ttatcaaacc ccaagctggc cagctttgag actaggttgg taagacctgc agccccattt
    10141 gctgcatttg cgccattttc tgtgaatgct gcataaaatg ttgcttgcgg agaaccgacg
    10201 ttaaatgtcc aagaatctcg cgctgtcgga tcctgattca gctgtatgtc atgaatggta
    10261 acaaaaactt cggagcctgg cgggtagtca gccctatcta gactcagtga gatgtttggt
    10321 atgtcgtcat attgtaaggt tacttgttgt gtaccacctg ctgcgttgta ctgtattacc
    10381 acattgctgc taaatgaata aagttggatt attggccatg catcatcatc caaaccaatt
    10441 tgtccagttg ggacagctga gtttgtgtta attgatcttg gatttctaac cacattattt
    10501 atgattgagc ctgacggtgc gccagtgcac gtgttaaatc cagagtttcc atttgttgaa
    10561 ccagtcatag tgtcttttgg taatgcgatt ccgtctgttt ctgaaaatga tgggccaagt
    10621 gatatggtgt ctcttccaca aaagacgcca aagtccaaac cttcgccagc aactcccgca
    10681 tcaagggaaa tctggtctgc aatctttgcc ttgtcaacat ttgcaaaata tgcgtaccat
    10741 tttccatctc ctgcctgaac catccttaga gcatttccgt ttagtgtaac gtctggctcg
    10801 ccctttcctt catcagtatc agaaaggttg ggatcactaa ctacgacctc aattaccatc
    10861 gagccggcaa aatgattgtc ataaaacggg ttttctgcag acacgtacaa gtttgggttt
    10921 gatgccgcat ttgcctctga taacaatgga attagtagta accctaatgc cgccagtacc
    10981 atcttgttca atttattttg attttgcatt cataaaagat aatgttatcg tataatttat
    11041 ctcaaagtag gttatagaaa cacgacgaga agatcaccca ttctatcgaa gattttctaa
    11101 atagatggat cgcattccta ctcacgaaga taccacccga ttgtatgtta gttgatattg
    11161 cacacatcag agattacaat cttttcagtc ataattgttt taggcatgca tgtctaacta
    11221 aaaccattga cagtgacaag aaacctcaag ttcagactgt atccgaatca ttcccagtgg
    11281 aaaaggctgc aaaacacgct tggtgcatgc aaatggacct acaacaagat ggttgaaaaa
    11341 ataaacaatg agggattcca gacaagaaac gacctcaatt attttctgac cgaattaaag
    11401 gaacaggaat cgtggcttta ctcatatcat tccaaaatgc tccagatgat atcaacgcag
    11461 atagatgggg cccagaaatc cctgatcgcc ttgaacaaaa aaggacacaa ggtaggcaag
    11521 ttgaaatttg caaaatacat agaataccgc acattcgcat acaaccagtc aggatttgaa
    11581 atcaaagatg gttttctgca tctctcaaaa ataggaaaaa tcaaaatcat gcagcaccga
    11641 aaaattccag aaaatataga aatcaagcaa atagcagtaa caagatcaaa aaccggcaaa
    11701 tggtattgtt ccataacatg tgatgttgat gccgtactga caaaattcag tatcacaaaa
    11761 tcagtcggaa tagatgtagg aatcaagaat tttgcatatg attcggatgg atttgtaact
    11821 ccaaacccat tgaatctgca aaaaatgctc aagccattgg caagagctca gagaaagata
    11881 gccagaaggc aaaaaggctc aaacaacaga aagaaggcag taagatggta tcagatagtc
    11941 catgaacgag ttaaaaaccg cagaaaagac ttcttgcatc agatatctac acaatatgca
    12001 agaaagtatg atgtgatatt tttggaaaga ctgcaaaaac tgaatatggt aaaaaaccac
    12061 aaatgggcaa gaaacattct agattctgga tggggtacat tcacaaatat gctggactac
    12121 aagtgcatgc tagtagaggt tccagcaaag aatacaacca ttgactgctc aagatgtgga
    12181 aatgcagtgc caaagtcttt ggtcgttcgg actcacagat gtgatgtatg tggcctggtg
    12241 cttgacaggg accacaatgc agcaattaac attttgaaaa agggattaga tattttcaac
    12301 attaatctac cgcaggaact gcgggaagtt acgcctgtag agatcatacc atggtcagtg
    12361 aagcaggaag aggctaccgg acgtctggta gtggttcact agtaagcttt gaaaatagag
    12421 ttttaatgca aaacgtcttt ttacaaagga caaatgccct aaagatctcc ttaattgcaa
    12481 tactgtctgc atttgtagtc gagtttgtct ttggcataat ttccaacagc cttgcgttat
    12541 tgacagacag cattcacgca ttattggata gtgttgtgac agttgtgctg ctgatggcag
    12601 ccaggtttgc cataaagcca ccagatgcag aacacacata cggtcatgga aagattgaat
    12661 cacttggcgg cctaattggc ggcattgcga tatttctaat tgcaatcttt ttcatttatg
    12721 aggcaattat cagaattcaa agccctcctc ccacaatagt cccaggaatg cttgcgcttg
    12781 ctgccgcaat ttacacaata ggtactgatg tttttaggat tactttacta aaaaaggcaa
    12841 taaacaaagt tggcggcacc acgctcaaag ctgactttta ccacgcgttc atggatttgg
    12901 gctcaactct tgtagtcatt gtggggataa ttctagttac ggttggtttt catgatggag
    12961 actttgttgc agcactgatt cttggagtgc tgctttgctt tctcagcctt aagctagtct
    13021 acaaaacagc gcaagagctg acagatgtta tttcacctac gctggtaagt agggtaaggg
    13081 agattgtttt gtcaacagac gaagtcctcg atgcaggacc aatccttatg cgaaagtcag
    13141 gcgatttgat ttttgcagac gtcaccatat cgctgcgtgc cgacgctagc tttgataggg
    13201 ctcacgagat tagcagccga gtcgaagaaa atatcaaaaa atcgatctca aactcggaga
    13261 taactgtcca ttttgagccc agctggcggg atgtgccaag ggattcaaaa atttatgaag
    13321 ttgcctcaaa tgttccagga gtcaagggag tgcacaatgt tagtcaacat acctccgata
    13381 gtggcatatt tgcaagcttg cacgtaatgg ttgacaggca gatgagcctt gaggaggccc
    13441 acaaggtttc agaaataata gaaagtaaaa tcaagcagtc actgccagaa atagagaaca
    13501 ttacaattca tctggagcca tacattgcca taccaaagga tctaaaagga cacaaaacat
    13561 cagatgagaa aataattcag ctgttaaagg ggcatcccga agtcaagaga gttggacgca
    13621 tcgtgacgct gaattttgat gagctgttaa aaatcgacat caattgttcg tttgacaaaa
    13681 acctgtcaat tgaaaaggtt cacgatctta cctcgcaaat cgagcaaaac atacgaaatt
    13741 attttaaaaa ttctgtcata acaatacacc ccgagccgta ttagactaga atgcttgtca
    13801 gatacatgat cagcatccct atggcaaatc cggatgctac tgcggcactg gagaggtttt
    13861 tgtcgccttc ttctcgcatc cacctcagta tggtaattgt tacttgaaat atggcgcctg
    13921 cccctattga gagaaaaact accgaagtaa atggagagta aacaaacccg cctatccatg
    13981 caccaaaaat tgccggagct cctgcaatca ttcccagtcc tgcaagcttt ccaatcattg
    14041 tttttcctcg tgacattggc gccgcaatgg caataccctc agttgtattg tgcaaagcaa
    14101 atccaactat tagaaaggtg ctaaacgcaa tagagccaag accaacagct gcacctattg
    14161 caagtccttc gccaaaatta tgcagcccga tgcctattgc aatcattaat gcaattgcca
    14221 caggttttgt cattctagat gattctgttc tttttactaa tttctcgcca gtatagtata
    14281 acccaaagaa ggacagcaca ataactgtgg ccacaagcaa tgcgccatta aagctactag
    14341 caagactttc tctagaaact tctagcgcct cttcaacaga atcaatgcct aagaacagta
    14401 aaagtcctgc agtcagtgcc aaaaaaaagt gatacttgct tttgctaatc tttttgataa
    14461 atggaagcca aagtaatcct atcatgacag gtattattcc aacataagtg ccgattatag
    14521 caaaaaatcc agcaagctca agacttggtt ctaatgcagt agctgcagct tctatttctt
    14581 tttcaaatct tgttccatct tcgatggtaa ttccaattat gtagggttgt gcctcgttcc
    14641 attcaaaggg tattcttacc agagccgttt catatcgctc cagaaatcta tctggctcta
    14701 ctgctgcagg ttgtattctg tcgttgatat ctgccatcgt aacttcaaca ggtattggtc
    14761 ctgtgtttct tacagttgct cgaatttcat ggtccacaaa atcaaccttt tctattgtta
    14821 cctcgggtag tgctacacct aaatccaaaa gttcagagcc tggaccaaag atatacgcca
    14881 ccaaaagtat gagaagcgca aatggaatta ttccacttgc gatcattttt ccttttgatg
    14941 atttgttgct aagttccata tcctatttcc ccttgtgatt tttgatctgg attgtccttt
    15001 actaggaata ttccaaccca gcccttttct gagaattcga ccttatgagc gtggaataga
    15061 tattttcctg ggtatgtata ttcaaattcc atgattcctc tctcagtttg agacagcgtt
    15121 atcatgtcag tgtatgtaga aggaaccgta tcagttcctg taggataata atagtatagg
    15181 ttgccgtgca ggtgcagatt gtttattgga tcaaattcca ccatgtttac aacataaact
    15241 ctgattaatt cattagtgtt aatttgaatt ggatggtgtt gatagtaaaa cggaattgtg
    15301 tttgctgcat agaaattatt ctcagtatca aaatcagtat caaagccgtt tagaaccata
    15361 accatctcat ctgctgccgg tctgccttct tttggatcaa ctatgtaaac accatacagg
    15421 ccatgcacga tgtgttcctc aagtggcatc acatgacagt gatatggatg aacgcctaca
    15481 ggccctgcct caaactcata aggaaatcta ccacccccag accctactgg ctcaaacacc
    15541 ccatccattt cagctcgatg gattccatga aagtgcatgg tatgctcttt tgagccattg
    15601 ttgatgaagt atatgcgaat aagatcgcct tctgtggccc ttatagtcgg acctggaacc
    15661 gtaccattaa aggtccatac attatagaaa acacctgggg agatttccat aattttatcg
    15721 tcttctgcaa taattgtaaa ttcgcgcaat gttctgccgt cttctaactt tgagatgcgg
    15781 ccatagttga attctcgaag atatttttcg ggatcaaact cgactgtttg tgcagtgtat
    15841 actggatcca aaacctcgcc tgatgttttt atgactgcac cagtatgcgt gacaaacgtt
    15901 ttttcctcgt ttttggcatc tgagatattt gggtagacaa agtataacgt ggcaagtatc
    15961 gtagttacta ctaggatgga tgtcattttt gaaaatgctt tcaagatcta tacgcataat
    16021 gaccattttt tgtctattta aaagttagcc taggctaact tttttagcca agactaactt
    16081 ttttgattca accatcataa aaaacattct caaaaatacc tcaatatagg accccactga
    16141 gcggtgtgta atgagaaaat cccatctaat cttgttcatt gggggcgcac ttgtagcaat
    16201 tggcatggta acttcgtttt atggatcaaa tcttgtaacg caggacctcg caatagttga
    16261 gggagtggca agcgatgcct cgccagttga gctgataaag gaattagatc cctccatagc
    16321 aaatacaggt gtttttgtcg ttcaggcaga aagttttgat ggattagttg ccacaatatt
    16381 agatccaatt ggacaacaga taacatcaat tgagatcagc caaagatcaa cagaggagca
    16441 gttcgaaatt acggtaaagg gaagctacaa attattattg caaagttcgg ctacaagcga
    16501 agttccaatt gtaatgggtt tgacacacat gccagacaag aactttattg cactaaacct
    16561 cttagggcag tcgctaatag tttccggatt tgtcggagtt ggactcgctg taatattaga
    16621 aataaaaaac cggaaaaaga aaatcagtta agatagctgt caagctcagt taggccatca
    16681 actacgtttt tgtagttttc atcactttcc atgatttttg caatcttgtc tgcgttgaca
    16741 gtgtatgata aaggagagtt tgatgatatg acaaaaccgg ccccgattag gtagccaagg
    16801 cgtgttttga tctcttcttc tgagattcca atcttgtttg ccagataggc tgattctttg
    16861 ctaccattct ccagctctgc taaaatgatt gatatgtctg ggttgaacaa gtcctcaagc
    16921 accttgccct tgtcatagtt ttccgtcatg gcgtgcctag ttttttacac ttaatatttt
    16981 cttggtgttg tacagatagt caatgactag gaccttttcc atgttgtttt gatttatgtg
    17041 ataggttttg ccttgcagtt gtttttccat ttgcttgaca tagtctagca gctctgcttc
    17101 ctcgctgact acaattgtgt caatttggat gttttcgcgc ttgcattttt ttgcctcatt
    17161 aatggtttgc tcgtttactt ttggatctac ctttttgtat ctatagcaga ggtaaaccat
    17221 gctgccactg tcaattttca tgttgcgctc ctctcgtatt tttgatagca atgatgggtc
    17281 tggctcataa aaattggagt atggtttttc agatagtatt gagttttttt ggttttcagt
    17341 atctacaaag catgcacttg gctgaccgtc cgttatcatg acgattcgtt tgtttttggc
    17401 ggaatttttt tgtagaattt tttttgccag tctaaatgct gcctgatagt tggtatagtg
    17461 caaaaaatca tcgtttgcgt catatgtcct caggaacggt atgtcaaatg gatccacaat
    17521 ggatgccatc gaagcaaagc cgacaaccga aatagaatcg tttggaaaga acttgttgtt
    17581 tagaacgtaa aggctccata gcgctctttt tgctgcctcg attcgactaa tactggggcc
    17641 agtcagagaa gacttcatag tagaactcaa gtctatgcag tagacaactg cagtccttac
    17701 atcctcgatt gtttcaaact cttcaaagtc atccacatct atatcaagtg gaaattttaa
    17761 ttctgatcct ttctttgtga gtcggcttat ggtgttaagt atggtaattg gaacgctcag
    17821 tagtttcagc tcatcgccaa gctcaaactt ttttgtagta tccaaaattg tcgtgccaat
    17881 gcctactgac ttggtttcat gtaatccaaa ctcgcctgtc tttagctcat tcattagatc
    17941 acggagaatt tggttgccta ttgcaaagaa ccccttcttt gtaagccagt ttgtgtcgtc
    18001 tttgagatag ccctgttcta ttaggtattt tgtgatatgg tgtatatcgt cactttttgg
    18061 aatttggtct gattgttcgt gcggcaccga atctgagctt agttcccgta tgttagttag
    18121 cagaatgttt tcaagttcat ctaatgttgg tgctttttcg cgcatcgatt ggctcgcaag
    18181 cgttttgaga attcgctcta gtgttttttg gtcaagggtt cgcgattttt cattggaatc
    18241 ttgtcttttt tcttgcgtgg ccgaaaaata cacaaaattt ttgaaattat gcagcgacat
    18301 aaccagcttc ttttttatca attatctttg gcgtgacaaa gcacaggccg tctaagacta
    18361 cctctaagac tgcggccttt agctcattta cggcttgggt tccaagtgtg atgtttttgt
    18421 ttgggatctt gtctgcagct agtttttcca caaagcttga ctgttcttgt tttatttgtt
    18481 ctagttttga gattatgagg ttgttaagct gggtaaagtt ttgcagttgt ttttcatacg
    18541 aggtttggcc gtttgaccaa acaatttttt gcgagacctg gaatgtcttg ccagtaaatt
    18601 cctgctttat tgattctaga atttcggcat caatgccttg cagatactca aggcatgtgt
    18661 ctttgatcgc actgccaaga agttcgccaa atatgtggtg gcggttttca agtgtatcat
    18721 caatttcaga aagctcaaac ttggcaattt gtgcaagaca gtacatatca gagtgccttg
    18781 gtactgccac tgtttggtag gggattgctc gcgtcctctc cgactcgcca actagaagct
    18841 ccaaaccatg tatgccaaat cttacgctta cgcccttggc ttgatttatt tccggattgt
    18901 tgcgtgcttt ttgcaccaat cttgtaaggg ttcttaatac aaaaactgga ataaagcagt
    18961 tggctgtttt tgcctcttgt aaaattatca gcatttcatc atccatggtt tttggataat
    19021 gggtgtgcac atgactcttt agcctatcat aaagcggttc aatgattttg ccagaatgtg
    19081 tgtagtcaat tgggtttgca gtcgcaaaaa agacagtctt tggctcaaac attactggat
    19141 acgagcctgt tgtgtatctg ccttcttgca atacagagag tagtgcaacc tgttttcttg
    19201 gatccaaaac cggaagctca tcaatgcaaa atattccgtg ttttgcctgc atcagttgtc
    19261 ctggcgaata tgattcaatt ttgaacatct ctacgccttt tttggcaacc ttgattgcat
    19321 caatgtaccc aacaagatcc tttaccgata tgtcaggtgt tgccaagaca taccggaacc
    19381 tttgttgccc gtcaacccat gtgatttttg tatctagatt attgtcaaga attttttgag
    19441 agctctcagg gcttatgtag aatttcggtg atgatttgtt ggcttcttgt ccttccaata
    19501 atgaaatcag ctcatctgct ggcaggtcca ttgggcagtc acttgttatg ctaccgtcaa
    19561 ctattgggat tggcgataaa agattctttg cgattgtctc gactaatttt gttttggctt
    19621 gtcctatttg gccaatcaaa aagatgtcat gcccagaaag aatcgcccta tccagtgcag
    19681 ggatcacgtc ttcgtcatat cctagaattc ccggatattt tttctcgcca gatttcatgg
    19741 cactgataag attttttcta agctgctctc ttgcttcaag aagcttgtaa ttaatttcaa
    19801 gaagatcccg tattgttttt atgtcctcat agtcttctgg aactcctgct tttatttcgg
    19861 tgtatttggg ctcagagctg tagcatctct gaacaagttc tgaaactgct gtcaatcgct
    19921 actgttacgt tatgttatga tataatatta gtgaacgaat ctttgctcaa tattcgggta
    19981 gggtttttat cggggttgaa ttgattttcc aatacttgtc agcacaagaa tataggcaca
    20041 ttgttaggat tgttggaaag gatgttccag gagacaaaaa gctctcagtt ggacttgccc
    20101 agattaaggg agtaggctac aattttgcca atgcgctaat tggcgctttg gatttgaacc
    20161 caaatagcaa cattgggttt cttacagaat ctcaagtaga agtcattgaa aaggcgctaa
    20221 aagatcccac atctgttaat tttccagctt ggatctacaa tagaagaaag gatgtcgaat
    20281 ctggaaatag ttttcatcta ataacatcag acgttgcatt tacagtaaga aatgacattg
    20341 agagggaaaa acaggtaaac agctggcgag gttttcgtca tacttttgga ttaaaggtta
    20401 gaggtcaacg aactagaact actggtcgta agggagcatc agttggagtt aaaaagggcg
    20461 gtaaagtatt acctgcaggt gtgctacctc caggagcaga ggcaggtgca gcaccagcag
    20521 ctgcaggagc agctccggca gcaggtgcag caccagcagc cactgcaccg gcaaaagctg
    20581 cagcggcagg aaagtctcca gctgcaggaa agacaccggc tgacacaaag gcaaaggcac
    20641 cagctccgga tgcaaaggca aagaaatagg tgaaatgatt gggagatcct cattatccaa
    20701 gtagaatctg gagaaaacca aaacgtcctc taaattatga ctttatgatg gaggatctca
    20761 acactttggg aacctatgga ttgaaaaaca agcgagagtt atggaaggca agaactgagc
    20821 tatcacgagt cagacaccag gcaagatcac ttttagcact cagacaagaa ataagagaac
    20881 aaaaagaacc aatcttgatg aactcactga caagaattgg acttgtaaag gagggcgcca
    20941 ccttagatga cgttcttaac ttggatatcg atgacttgct ctcgcgcaga tttcagacaa
    21001 tggttcagaa aaagttctca tttaagacgc cgtaccaagc aagacaggcc atagttcacg
    21061 gacacgtaac aatcggagac agagtcgtca ccatcccgtc atatattgta aatatcaaag
    21121 aagaagggct cattcaacta tcgccaaaat tcaaagtgca aaacaatccc ccagccgaag
    21181 caaaacaaga agagtcagag cctcaatcag agtctcaatc ataaacgacg cttatatcta
    21241 aatagctgat ttgttaaatc actgcagaaa atgtgttcta taataggata ttatggcgat
    21301 gggttggcgg cacccattct tgtaaagggc ctaaagagaa tggagtaccg aggctacgat
    21361 agtgtaggtg ttgccacaaa gtccaaccag gagattcttg tcaaaaaggg agtaggcaag
    21421 gtcgcagaag taaacagctc gcttcaccta gattcgttgc caggaaatat tggaattggc
    21481 cacacaaggt gggctacaca cggtaatgtg actgatgtca atgcacaccc acacccaagc
    21541 agttctggtc aaattgccat agtgcataat ggcatcattg aaaactatga tgagctcaaa
    21601 gtggatttag aaaaaaaagg ctaccagttc aaaagccaga ccgacagtga agtaattgcc
    21661 aatttgttgc agttcaatta tgaccaaaca agcgacataa aaaaagccat gattaaaacg
    21721 attttaggac tcaaaggtca ttatgcgttt gttgcaatgt ttcaaaacgg aactctctca
    21781 gctgccagac atcatgaacc cctgataatt ggtattggaa agcacggata ctttttgtcg
    21841 agtgacgttc ttggattcat agaaaaaact gatgatgcga tttatcttga taatggcgag
    21901 tttgtactac ttgacaaaat tgggattcag atatacaact ttgaaggcga gccggtaaaa
    21961 caccaggtaa caaaggtctc aaaggagttt gcggatgcat acaagggcga ttatgcgcac
    22021 tttacactaa aagagatctc agagcagcca gagacaataa ttcaggcagg cgagagttcc
    22081 atggatgcaa taaacgcagc cactgatttt atcaagcatg caaaaaatat ctacattact
    22141 ggtagcggca caagctacaa tgcggcacta gtcgcaaaat acctttggca aaaacatgca
    22201 aagataaagg tagagccaat aatatcaagc gagctttctt tttcacctga tgctattgag
    22261 ccaaactcca ttcttgttgc catctcgcaa agcggtgaga gtgccgacgt tttggaggca
    22321 gtaaacattg caaaaaaatc caacgcaaag atactgtccc ttgtcaatat tttgacgtct
    22381 tctctggctc aagagtcagc actcgttatg ggaatgagtt gcggcccaga aattggcgtt
    22441 gctgcaacaa agagctttac atcgcagctt agcgtcattt acaagataac ggaaaagctg
    22501 tgcgatggct gcatcaaaat tgataacgcc aagatctcag atgcagttgg agtggttctt
    22561 tcggatcatt caaagataaa gcagatagca aaggatctta gggacgttac agacatttac
    22621 attctgggta gggccgtcca ttatccaatt gctgccgagt ctgcactgaa gctaaaggag
    22681 cttgcgtaca ttcatgccga aggcatcccg ggtggtgagc taaaacacgg cccgctcgct
    22741 ttgatggatt caagcgttta tgttatcata ataaatccaa atgactttac ctacaatgat
    22801 acgctcacaa gtgcaagaga gatcaaggcg cgaggtgcaa aaattattgg aatttctgat
    22861 aagccaagcg acgtatacga ttattggatt tcaattccaa ctattgacga ggcgttttat
    22921 ccgctaatag agataatccc aattcagctt cttgcatatt attctgcaat agaaaaaaac
    22981 acggatccgg attacccaag aaatcttgcc aagtcggtta cagtaaaata gtgttttcgt
    23041 actgttgttc ggtcagctgc aacaaatgct ttgagtcaat tccaactttt agcattgctg
    23101 caagcgatgc accgcctgct ccaactcctt cctttacaaa tccgtcggca aacgatctta
    23161 gaccgtttat tttagagtcc ttcagagcaa gcctgacaga aaagactggt atttggtcga
    23221 tttgctttat ggtatcaact aggtttgcag tgttatcatc gattacataa gaggttgtgg
    23281 caattgccgt gttgtctttg tcatatcctg tgattttact aaatgccaaa acagctgcca
    23341 tttgagttcc tcctgccaaa atcactttgg ttgatgacga tgcagtgcta agaatcccag
    23401 caattgttgg gatcatcgga tctccggcgt ttgcaacaac atcaaatgga tttttggaat
    23461 cgattcgttt tgaggcagta tttgctattt cgtttttgag tgcaagcggg ttgtcctgca
    23521 tgctgctact cacctttgcg tcaattccaa gtgaggcaag cactgcctgc gccgttgtgg
    23581 ttcctccagg tatgctttca cctagcacca gacagtcagt tatggagcca aggcttcgcc
    23641 caatcattct cccatattct actgcgtgtg taactgcatc aagtggcatt gccggacctg
    23701 ttgctatgtt tttgccaaaa ggaagcccag tctcaaagta cggcaacttt ggcagtatct
    23761 tgctgcctgc attaatcaca atgtttgaaa tgctagctga ctctagtgcg gtttttgtca
    23821 gcagcgccgg agtcggcttg ccatcaggag tcattgggat tacgtttatg catttgcagt
    23881 aaccaaaatt caaaaattct gcgtctgcag gagaagtata ctgcaaaagc gccgggtttt
    23941 ttccagcaac tgtaattccc ggaattgtgc aggtatcagt atacgatata accaaagaaa
    24001 aaagaaaatg ctttttttca actgattcta aaaactcgcg gcctttttgt tggtcgacaa
    24061 gaatgtcaat gtcttgattc aattatccta ttcccatgat ttgtagaaat tcctgctctg
    24121 ttcttggctg cattacaaaa tttgtctttt tttggtttcc aagtgtcgca gtaggagttg
    24181 agccgtagtt gtgacctggg tacataatca aatcatcgtc cagtgttcgt aaaacatcaa
    24241 cgaggctgtg atacagctct tttgcatccc ccccgggcag atcaacgcgg ccacaagtgc
    24301 caacaaagag agtatcgcca gagaaaatct tgccatcacc tacaaggcac atgctgtcct
    24361 ttgagtggcc cggagtatgc aagacgctca gctccgagct tccaaacttt atcttatcac
    24421 cgtctgatac ggcaatgtca tgctttagtg ttgaggtttt gtactggatt atttttgcat
    24481 ctgtttcttt tgccattgtt tcattgccaa gtgtgtgatc aaagtggtag tgcgtgttta
    24541 caatgtattg tactttgagg ttgttttttt caattactcg ttttagatca tcaagatccc
    24601 acgacgggtc tattattatt gcgtctccag cgttctcgtc ttctacaacg taggtaaaat
    24661 tttgcatgtt acctacctct aattgatgga ctatcataat acgccgatct ctgcttctgg
    24721 tggaatgcca attttttcaa caaaaatcgt tccgcaaatg tctatcctct ttggcatgcc
    24781 gaccttcatt ttatgaaacg ttactgtcat gtcagctttg acgtacttgt ctgcagtttg
    24841 gccagtatct ggattcatgc ctgttggtac atccactgat attttgaagg cactcgactt
    24901 gttaatcaga tcaattgctg acgcttgtgg ctccctaata gttccagtaa ttccggtacc
    24961 aagtatgcca tctactataa tgtcgacatt aaagttggag gcatcatctg gtccaaaacc
    25021 aagttgtact gatttcattt tctcaagaat ttgccagttt gttctgcatt cttctgtttt
    25081 gatgcctttt ggattgccaa gcaaaactac aattactttg cagccaaatc ctgcaaggtg
    25141 ccttgaaaca actagtgcgt ccccgccatt gtttcccagt cctgcaaata caagtacctt
    25201 ttttgatgaa atgtctggaa atttgtttac tagttgtttt accattgccg cacctgcatt
    25261 ttccatcatc agctgacgca aaaatcccat ttggtggccg ttttcttcta tttggtacat
    25321 ttgttttaca gtgatttcca tgatgaaatc ataatgtgga cataaaataa gccatttgga
    25381 aaacgacttt atgctagttt tttgtggttc ttacatgcta gattccacaa agcgatcact
    25441 tgagaaaatt tcaaaatctc ttgagaaaaa ccttgagggg cgtgagagcc tgatcaaaaa
    25501 cacccgccaa atagtgattt tgtgcagcga gtcaataatt gactgccaca aaaatgatac
    25561 caagtctgct gcaaaaaaag tacttgccgc agaaaaactg ctagcgcagt accgcaaaaa
    25621 aactgctgag ggtttgtaca gatatctttt gctaccagaa caagaattcg tagaggcgag
    25681 ttcgcttttg tcaattctgc aaggaaacca agtacccgct atagaatcac tccatgtaaa
    25741 agaagagtcg tatgttttgg gtttgcttga ctgcattggg gagctgagaa gaaatgtcta
    25801 cgataagata agaactggtc atgccgccga ggcaaaaaag atgttcgaca taatggaaga
    25861 gttgtatttg cagttgtatc cttttgcgta ttttgataaa atagtaaaag aagtaagaag
    25921 aaagctagac gttgccagaa ttttagttga ggagacgcga attgcaataa ctgaggagat
    25981 cagacgctct gaattaatca aaaacatgaa gaagtccaga aaataatcta taattcaggc
    26041 gttttgtgtt atcgtagtgg gattagaata gatggtacag atctaaagta ggtagaaaaa
    26101 aatcaaaatc gcttcggaga aaacaaagat tcaagatctg ttatccctcc catacaaatt
    26161 tttgtgatcg tcaagatcaa ttagataaac tgttcgtttc tcaaaatcaa catagtagat
    26221 taaactgtgt gattttgtct gtgttttcca aaacacttcc taaagcgtcc ttgttttggt
    26281 tctcccatct ttcttggatc atctgcattt gtaagatctt cgatgaattt gaaatagttg
    26341 tcagttaact gcttatttgc cttgtatttt tctagtttct ttgatattgt tctatcaacg
    26401 gaagctattt tccacaagtt ttattctttt ataatttttt tagcatgctt tagatagtct
    26461 tctggcgtat catagactgt aactttttct tttccagaaa caacacgttc taaaagatcg
    26521 tcaagttctg gatttaactc actttttgcc tctttgtgag atttttgcat atcatatcta
    26581 taacacaaga tctttataac atcttggtat gtaatctaga gttttcttca taaattatta
    26641 tctgagagat caaaccgcta aaggattaaa acatcacacc agaaacggtt tcgatatgaa
    26701 tcacaacatt tcacgtattc aattttagtg tagaataaca ttggttattt gaatcagttt
    26761 gcttgaattt ttctgctaat tctttataga ataactttgc tttagatttg ttgctaaaca
    26821 atatctctac aagctcacct acacgaataa gtgttagtga agttgttcca gtggcatact
    26881 gattagtttc catatcttta atcatttgag taataatggt gttcgtagga tttaatggat
    26941 tactgtgaat attgatgcat gttttgtgaa catgtaatcg atcgatatgt ctttctagcg
    27001 gaaattcatc gatatttgtt caacatattg tgaatatttg aaaataatta gaatgcggga
    27061 agtgggattt gaacccacga aatcctaaga tactagcccc tcaagctagc gccgttggcc
    27121 gggcttggca actcccgcgg taaacttcca tgcagggttt ttataacctt aattactttt
    27181 tgagttcgtg ttaattccag tcagatgttt tacttgcgga aacctaattg ctgacagata
    27241 tgaggaatac cagaacagaa ttaaagctgg agaagagcca gccaaggttt tagactcact
    27301 tggtttcaaa agatactgtt gcagacgaat gctgctcacc acagttgaga caatacagca
    27361 aatcattcca ttttacgagg caattcaaag gcgatatttg gaaatccaat ctgagttaga
    27421 gtagcttgcc aaagatctca tcgataagat gcagactagt ttacaatagc agaggaggta
    27481 aaacaatcga agttgatgta atttctgatg gcaaacacct tggaagggct tgtgtgcctt
    27541 cgggtgcaag tgttggaaaa cacgaggcgc aaagttttgc caacaacaag ccagagtcaa
    27601 gcctgcaaat actcaaaaaa aacctcagaa aatttgttgg ccttgattca aacgacctaa
    27661 aatcgattca aaaggtgctg cgtcaagttg acaacacccc aaattattca aagataggcg
    27721 gcgctgcagc atacgccata accattgctt ctgttgattc tgctgccaaa gcccttggcg
    27781 tgccaatgtt taagctgata tctcagacaa aaaaatggag attcccgttt ccacttggaa
    27841 atattttggg tgggggagcg catgcaggtc ctggaacacc agacattcaa gagattttga
    27901 tttgtgcagt cggtgcaaaa tcagtcagag aggcaatcga aacaaatttt gcagtgcaca
    27961 aggagttggg caaaatacta cgaagtgagg atcctcgatt taccaatggt cgtggagacg
    28021 agggcggctg ggccccagaa ttagacaatg agagggcttt ggaggtctcc gcaagggctt
    28081 gtgagaaatt gggttacact ctaggaaaag aagtcgcact aggtgttgat tttgcttcat
    28141 ctacgcaatg ggatgagaaa aagaaaaaat atctctatga gagggccggc tttgagaaca
    28201 cgccagacga gcagattgat tttgcagcta atatcataaa aaaatacaag ctggtttatg
    28261 cagaagatgc ggttcacgag gaagcgtttg accagatggc aaggctagct aaaaaattcc
    28321 caaagacact cattgtaggc gatgatctca ccgtaacaaa tgccaagatt ctaaaaaagg
    28381 cgattgcaaa aaagtcttgc aatgccgcaa tcctaaaggt aaatcaggca ggaagccttt
    28441 acgacgcact agaatttgcc caagtggcaa caaaacacaa cataagatta atcacatcac
    28501 acagatcagg cgaatcaact gattaccaaa tatcccacat tggcattgca actggttcta
    28561 agatgctaaa ggttggcgta gtgggaggcg aaagggtagc aaaactcaac gagttgattc
    28621 gtctctcaga gtataactta atacacggta tggcagagat ttaaaatcgc gatgagtcag
    28681 caagcagaag ctcaagacat caaaaagaag atcctatcca caggaattag ggtagggaca
    28741 actgtaaaga ctacctttat gcagccgttt atcacaaagg caagcccaga gggcctttac
    28801 atgatagatc ttgacaagac cctcgcaaga ataaagactg cagcaaaatt tgccaacaaa
    28861 atagaatttg ataaaattct agtctgctct ggaagagaat atgcttttac tccaattgag
    28921 aaattctgcg agttgacagg ggcaaaaatg atgttaggca gattcatgcc tggaacgctt
    28981 accaacccat ctctgtcgta ctatacagag ccaaagctgg tcataatatc cgatcctcag
    29041 atcgactccc aagcaataac tgaggcaaca aatgcaggca ttccagtaat cggagtatcc
    29101 aacacggata atgtgacttc aaaaattgat ctggtcatac ctgcaaacaa ccgaggaaga
    29161 aaatcacttg cgaccgtgtt ttggttgctt gcaagagaaa ttttgatcca aaagggacag
    29221 ctcagtgaga gcgacccaat gaaatacgaa atagacgatt ttgagacaaa gataaccgaa
    29281 gaggaaatgg agtaatgcgg attagaacac cggctgttgc tggcatgttc tatccaaaag
    29341 aaaaaaacga actaagagaa tcaattcaga gttgcatttc acacaaatat ggtgttggtc
    29401 agtcaaaaac agaacaactc aaagtttacg gtgcgatatg tccgcacgca ggctacatgt
    29461 attccgggcc cgtagcgagt cattcctttc aatatctttc caaccaagac tttgagcttg
    29521 ccataatact tgggccaaac cactgggggg ttggttgcag tatcgccaca ctaaaggact
    29581 gtacatggca gaccccactt ggagatgtag atgttgactc agaggccgca gtcgaagtca
    29641 ataaaaattc aaagctaata gagatagatt ttttttcaca cacaagggat cacagcatcg
    29701 aagttcaggt tcccatgtta caagagtttt acaaaaagac attcaagata ttgcccataa
    29761 ttttaaatga ccaagactat gagtttgcac tcgaagttgg cgctgccatt gccaagctcg
    29821 cagcaaagaa aaaatcaatc ataattggct catctgattt tacccactat gaggaaaacg
    29881 agtttgcgca cagccaagac aaagcactaa ttgagccaat tttggattta gatgtggaaa
    29941 aattctacaa agtactaaat gagaggaggg tgactgcgtg tggatacgga gcaattgcat
    30001 ccacgatggt tgcatgcaaa gaacttgggg caacacaagg caagctgtta cagtacgcca
    30061 caagtggcga tattgcaggg gataaaagct ccgttgtcgg ttacgcatca ataatattta
    30121 cttgaggtcc atagcgtctg cgccaggaaa agtcattctt tttggcgagc atttcatagt
    30181 ttatggcgga aaagccattt tgtgctctat taacaagcga ataacaatcg agtctcagct
    30241 ttcaaattca gataaaattg agataaactc agggcttgga acaattagcg tgtcaaaaaa
    30301 tgaacccctc caaaacatcg atcctgtttt taagccagtc atatatgtgg cacaaaagat
    30361 tcttcagaaa ttcaattctg attctggact aaaaatatca attacgtcag aaattccagc
    30421 aggcgttgga cttggttctt catcggcatg ctgcgttgct gcagcaagct caatttctgg
    30481 ccttttttca aattattcaa aagaagaagt tttgcagctt gcaatagagg cggaaaagac
    30541 aatctttgag aatgtgtcag gtgccgactc gactgtttgc acatatgggg gaataataga
    30601 atacagcaga gacggtaaaa tcaaaaaact tgattttgta ccaaagtttc agctagtagt
    30661 tgcagattca aaaattatgc attcgacaag ccaagtagta tcaaaggtaa aaaaattcaa
    30721 agatgacaat ccccaggttt tttcgatgct ttgtgacaaa gaggcttccc ttattgaaga
    30781 aacacttggg gcattgaaaa aaaacgattt gaatacaatt ggtgcaaaaa tgaaccaaaa
    30841 tcaggcacat cttgaaaaaa ttggagtctc aaatgatctt ttaggctcga tgcttggcac
    30901 cgtaaataat gtagcatatg gcgctaagat gactggtgca ggagatggtg gttgtatcat
    30961 tgcccttgta aatgacacaa acttggatag agtgattgga gcgttgcgct caaaaaatta
    31021 cgaatgtttt gcagcgcaaa tagacactac tggtgtcgaa caaaaggtaa aagatagtcc
    31081 gtaagtcaaa tagatacttt ttaactatct gtttttctga aaacaaatat gattctcatt
    31141 aagcttggag gctccattat tacaaacaag gaaaggcccc tttctccaag aattagtacc
    31201 gttgataaaa taatgaggca gctaaaaaaa atccaagaac cattcatcgt tgtacatggt
    31261 ggcggatcct ttggccatta ctggtctgtc aaatatgaca tgcatacaaa gccagcaaaa
    31321 tatgatcttc acggcgtatc aatagtaaaa aactctatga tagagctaaa caaaataatt
    31381 ttggatcgtt ttgtaaaaaa tagattaaat ccgtactgcc tgccaccgac tgactttatg
    31441 gatggagaca aaccaattcc aagcaaggtt aaagaaatta aaaaaattgc cgagtctggc
    31501 ctgattccag taacgtatgg tgacgcactt tggtatggtg aaaaaaaatc ttacattctt
    31561 tcaggagata gaatcatgag cattttagcc aagtccttgc gaccaagact tgtagttttt
    31621 gtcctcaatg tagatggcct gtattctgac ttgaagacaa aaaagctggt ttatgagatg
    31681 aaagaccacc aagtttcaat acaaaaaata tcaatggatg tcacaggtgg aatgagacgc
    31741 aaggtgcaag aagcgacaaa aatttccaaa atgggactaa aagtattctt tgcaaatgga
    31801 aacaaaccgg aacgaatcac aaatgcaata caaaaaaaca agtttgaggg cacgatattt
    31861 agaggctaat acatggcaga cgagcacctg atcttagttg atagtgatga taacccaatc
    31921 gggtttgagg aaaaggtaaa gtgccatctc ccaaatggca aattacatcg agcgtttacc
    31981 gtcctgcttt tcaacaaaga gggaaaactc ttgctcacaa gacgaagcca aagcaagatg
    32041 ttgtggccaa atgattggga tggtactgtg gcaagccacc caagaaagtc cgaaacctat
    32101 gtttcctcag ccgaaagaag gctgccagaa gagattggtg cagcgtgcaa gcttgattat
    32161 ctattcaagt ttgagtatca tgtgccatac aaggatgttg gctcagaaaa tgagatttgt
    32221 ggaacgctaa tcggccaggt aaacgagcct tttgagctaa agctggtaag agatgaaatc
    32281 agcgagacaa agtgggtaaa tggcgacgat ctagtctcag acatagaaaa aaatccacaa
    32341 tcatattgcc catggatgtt agttgcgttg tatttgttgc cagaatcgga caaaaaaatg
    32401 attgaaaaac acaaacatat ttttgataaa tggttaaaga cagaattcaa aaacaatctg
    32461 gaaaaatcac ttcaatacca ctttcctaca aacaattgga ggctcctaaa ttaatgacct
    32521 cgactttgcc aaagaatacc aagcttgtaa acaagtactt gggggcaaag cttgacggca
    32581 gtccagctga gctgtaccga gcatcggcac acctcatagt aaatggcggc aaacgcctgc
    32641 gaccatatct ggtcataaag agttgtcaga tgcttggcgg ttccacaagg gatgcgatac
    32701 cggctgctgc cgcaatagag atgatccaca actttactct tgttcatgac gacatcatgg
    32761 ataatgacga aatgaggcat ggggtgccta cagtgcacac aaaatttggt atgccgcttg
    32821 ccattttggc aggagacgtg cttttctcaa aggcatttga aaccgtctca attgataatg
    32881 ttccaaacaa caagacttct gccaaactcg tagcaagact tgccaaggcg tgtgtaaatg
    32941 tttgtgaggg ccaggttcta gacatccaga tggcagaggg gaaaaaaatc cccagtaaat
    33001 cagactatat caaaatgata gaaaaaaaga cctctgctct ctttgaggtt tcttgtgcaa
    33061 tgggcgcaat ttgtgcaaat acaaatggca gggacattgc aaatcttgca tcttttggaa
    33121 aaaatcttgg tgtcgcgttt caaattactg atgattttat tggggtgctc ggcgacccca
    33181 aggttacaaa aaagcccgta ggaaacgatc tgcgtgaggg gaaaaaatcc cttcccatct
    33241 tgcttgcaat acaaaaggca gattctaaga aaaaaagaat aattctcaga gtgtttggaa
    33301 accccaaagc cacaaaaaaa gagatcaatg atgcagtgca ggctatgcgt tcgctggaaa
    33361 ttgaaaaaga cgtcagaaag gtggctttgc agtacgcaga caaggcaaaa agatcccttt
    33421 ctgcatattc tggctctgca aaggctgaga tcatctctct tttggatttt gtagtaaaaa
    33481 ggagcttgta agatttggat gatgttgtga aatccaaaat aagaaagcta gcactgcaaa
    33541 acgcagcaga gcatggaaaa actcaggaca agatagtcct tgcaaagatt ttggcaactg
    33601 agccgcagct tcgaagccaa gtcaaggaaa taatgccagc cattttggaa attgtaagtt
    33661 ccgtaaatca aatgtcgcaa gaacagcaaa gaaaggaaat agaaaaagag tttccagatc
    33721 tactagcaga aaagccaaaa aaagaagaac gtgatggatt accaccactt gagggcgcag
    33781 aacacggaaa ggtggtaaca agattcccac cggagccaaa cggatacccg cacataggac
    33841 atgccaaggc tgcaataatt gacgaagaat atgcaaagat gtatgggggc aagctggttc
    33901 tcagatttga cgacacaaat cccgaagctg agaggctcga gttctatgca gcaataaagg
    33961 tggggctgga ctggcttgag ataaaattcg acaaaataaa gaacacgtct gatgacattg
    34021 agcttcttta caaaaaatgc ctagagatgt taaatgggaa ttttgcatat gtttgcacct
    34081 gcaagcagga aaccatttct gcaaacagaa gggagatgaa atcatgcaag tgcagcttgg
    34141 gcgcactaga gcaaaacttg gagcgatggg acaaaatgtt ctcaaaatac aagcaaggtg
    34201 aggcaattgt caggtttcgc ggagacatga agtccgaaaa cacggtaatg cgggatcccg
    34261 taatgtttag aattattgaa acaaagcatc ctttgctttc agacaagtac agggtatggc
    34321 caagctatga ctttgctgtt gcaatagaag acagcattga tggcatcact catgccttta
    34381 gaacaaagga atacgagcta agaaacgaat tgtactatgc aatcctggat gcattaaaga
    34441 tgagaaagcc ccaggtctta gagttctcaa ggcttgggtt tgagggaatg ccggtctcaa
    34501 agcgagtact caggccgtta attgaggagg gaaaggtctc aggctatgac gacccaaggc
    34561 ttccaacact tgaggcgcta agaaggcgcg gcatacgggc agaggcaata cggaaatttg
    34621 ttttgtcttt gggatttaca aaggcagata ccttggcgcc atttgagaca ttagagtcgt
    34681 ttaatcgaaa aattattgat tctgatagca ttcgactaca catggtaaaa aacccggcaa
    34741 gcatcaaggt gcgaggccta ccaaagactg tcattatgca gaaccatcca acaaaagaca
    34801 tgggaagtag aaccatagaa ctagatgaga atttttacgt agaacaagac gacttgaaaa
    34861 acgccaagga gggcgaccag cttaggctaa ttggtcttgg caatgtgaga ctaacaaaac
    34921 agcagcctca tgaagcagaa tatgtaggtg atgatctaac accaaacatt gcaaagatac
    34981 actgggtggt gcaaaaaact gctcaaaaaa tcaaaattct aattccaagt cagcttttct
    35041 ttggggagca gttcaatgaa aaaagccttg aggagattaa cgtttttagt gagccgtatt
    35101 accttgagct aaaagaggga aaagaggttc aatttgtcag attcgggtac tgtagaaagg
    35161 attctatgct gcaggcaatt tacacgcaca agtgatgtga tatgaagata ggaagatttt
    35221 tgattgcagg tagggaaact tatgggtttg taaaagacgg ccgtattgcc acaaaagatg
    35281 agatcatcac aaagactgga ataccactac cactgaacat caaagagttc ctctttgatg
    35341 ggtggtatga ggagatcata tctcacaagc cagacttgga gtacggagtc agtttagatg
    35401 atgcaaaaat tttagcgcca atcccaaatc ccccaaagat aatctgtttg gcttttaact
    35461 atgttgacca cgcagaagag caaaacctca caccaccgac agagcctgca atagtgctca
    35521 tcccaagaac tacacttaat gggacggaat ccgaaattgt ttgcccaagc tttgtcaaac
    35581 agctagacta tgaagtggag cttgccttag taattggcaa aaactgcaaa aacattagtg
    35641 aaaaagacgc aatgagtacc atatttgggt acatggtatt caacgacgtt tcggcaagag
    35701 acgttcagga acaagacaag cagtttggga gggcaaaggg atttgatacg tttgctccct
    35761 gcggcccgtg gattacgact agggatgaaa tttcagatcc ccaaaatctc aaactaatca
    35821 ccaagataaa cgggcatctt cgacaaaatt cgtcgactag taatatgttc ataaaaatcc
    35881 catcaatcat atcaaagcta agccaagtga tgaccctaga aaaaggagat gtaatatcaa
    35941 ctgggacacc ggctggagtc atactaaata agccaaacgc aacttttcta aaagacggag
    36001 acaaaattga gatggaaatt gaagggcttg gaaaattaga aaataccatc cgatttattt
    36061 aataaaatta ttaaagagtt aaaaccaaag atcatcatgg gcaaaatcat agtcggcaaa
    36121 gtatccgaca ttcctgcagg caaaatgcaa aaggtaaccg tagacggtaa agagattctt
    36181 gttgcaaacg ttgatggcag tttttatgcg tgtgacgata cctgcacgca tgctggagca
    36241 agtctagcag aaggaaccct tgaagggggc ataattactt gcggatggca cggagccaag
    36301 tttaactgtt caagtggcaa gcttgacaag ttcccagcaa agataagcga cctcaagtcc
    36361 tacaaaacac tagtagaagc agacaacgtt tttgtcgaag tgtaaggtgt agacttaaaa
    36421 caagtgttta caatacaaaa atgaatcaga tatgtcagac aaaaaggcgc aaaaccaaaa
    36481 atcatttgat cttgcacttc aaaccctaac ccaaatcgca tcaaatccta cgaccccaaa
    36541 aaacatcaaa aaaaccatct cagatctgat tacaaacctc aaaagcagcc aagacccaat
    36601 gccgattcgt gttgccaatg cgattagtca gctagatgac atcacccaag accccaacat
    36661 gccatcatac gtaagagtga cactctggca agcagtctca acgcttgaga gtataagaga
    36721 atagtctgtt tttccatgta agagaataaa tttctcaaac accaaatttc cagtatgaaa
    36781 attgaagaat atcttgcatc attgccgcca tccataatct caggcgagga tgttcagctt
    36841 tcagatgatt cagtaagaga ggtcttcaag tttgtcaagc ttggaaaaaa cgatgtcttt
    36901 taccatcttg gatgcggaag tgggaacagt cttgtaattg cgttagagga attttttgtc
    36961 aataaggcag tcggcataga caaagacgac acaaagattg ccaaagcaaa aaaactcact
    37021 aaagaaaaaa acctgcaaaa cgcagagctt agatgccaag acatccttga ttctgatttg
    37081 tctgatgcga ctgtgattct tttttggttt tctgatacaa aaacagttga aaagatgttt
    37141 gaaaggttct caaaattaaa accgggaaca aaaatagtta caatatttga tccgcttcct
    37201 agaattcttc cagaaatggt tagcttccca tacattgttc accaagtacc gtttagacct
    37261 gcaagggatc tcagagaaca actagttgcg atatttgaaa cagaatgcat tgattttacg
    37321 actgcgtggg agtttgctga aaggtacaca aaagcagttg gctcaccaga ctctggaaat
    37381 gatcggttcc ttacaattct gcagacactg atgatctgga ttaatgcaag aaacctaggt
    37441 ctgtcctgta cgcaggaaat accggcccca gtcaaaagct acatggagat cttgcagaac
    37501 tttttcaaca tcgaggtaaa acacctcctc aaatgaattt agggacaacc ttttattttg
    37561 aagaagacta gttttagtaa tgaatggaca aataagcatc aacgtatctg ccaccgatta
    37621 tgacaaatcc agcaaagaga taaccaatac actcaggctt gtagaggaga tggtgcacga
    37681 gacagacggt tttgtcgtaa ctgattcaga gtttgcgttt ggatggcatt tttacgtcat
    37741 ttctgtaaga acagacttgg tccaaaaact cgcagaccaa atgggctctg attttcaaaa
    37801 gttaaaggga aagacgcttg ataaaaaatt cctttcatgg cttggccaaa aaatggagca
    37861 aaaaagcatc aaagtcaaat ttgcaataaa agaagaaatg gagtcaagca aatacgggat
    37921 tttctgaaaa aaactatggt tctagcagga ttttgagatt ttctgatttt gccgcttcct
    37981 caaatgcttt ttgataatca gatattggat aaacggcgct aattaggtcg tcgactagaa
    38041 caagaccgga tgccaatgcg ttaattgcag gcctaaatgg cccacaacgg gagccaacaa
    38101 tggtaatttc gttaattatg gcaggcgcaa agtcaagctt gttttttgat gccatagttg
    38161 attttagtac aatgactcca cgtggcttta caagccgcat ggtatctgaa aatccgcttt
    38221 cacgccccgt tgcttccact acaatatcga attggtgttc atcggccgat gtgatctgtg
    38281 tttttgtatc aattcctatc ttttgcagca tttgcagttt gttttgatgc cttccaaagc
    38341 aagtaatgtg cgaattattc aaccttaaca cccttgcaat caattgtgca agacgtccgt
    38401 caccaattat tgcaattttt tgattcggtt caatgcggag ctgctcattt atctcaaatg
    38461 cggctgcaag tggctctaca aacactgctt gcctatcaga aatcgaatca ggaattacgt
    38521 gcaggttttt ttcaggaaga accagatatt ctgcaaatgc gccatctctt tttaaaattc
    38581 caagaacagt cctgttagga caatgccttt caaggccggt tgtgcaataa ttgcattttc
    38641 cacaccctgc gttaatttct ccaacaactc gttttcctac aagatttgga ttgtccgatt
    38701 tttctaccac acctacgaat tcgtggccca agacgccatc atatgacatg taaccgcgaa
    38761 tcatttcaag atcggttccg caaatcccag caagttttac tttgactagt gcttcgcctg
    38821 attttggttc tggataatcg tcccttaagg cgatctttgt tccatcaaag taaagcgctt
    38881 tcattgtttt tgtctatttt ttagctaatt tgtggcttgt cacaggttcg attatgatat
    38941 gtaggccctc gagggacatt gtcaaattag tttgataccc tcatattttg tttataatat
    39001 gtggaaaaaa ttaccgcgga attacatttt tgccttgact tttgcaagga tccgttctgc
    39061 tagttttatc gaactttcag catctttggg actcatcaaa tcaggttggt attcggaggc
    39121 attttttaga ccaagcaata atttgaattg cctttcaaca tctttgtatt cttgaggatt
    39181 cagtattcct ttaataagaa taagtacgtc tatgtgctga ctcgatgcac gctttgcttt
    39241 gcgggatgtg gctaatgcgt caagggcatt gatcgaactg tgaactgcat tcattaccgc
    39301 attatcatac gcctcttttt gcatggcaat ttttgcaatt tcaagggagt ttattgcttt
    39361 tatgagatac ccgatgcttt ttttggatct atgtctctcg tcatttcaat acacttgcta
    39421 gttcttttcc acatatgagg atgtgattat ctaagattga acgtacaaat gtgttgtttt
    39481 tcttttttga tcgaaattgg gattcagaga aaacaatatg tgaaacctta ctctgaaatt
    39541 ttagtgagat ctctagtgcc gcatcagaaa tggcagaaat tgcgtgatca agatcattag
    39601 atattactaa gacgtcaata tcactgtcat ctcgctcttc cctttttgcc acactgccaa
    39661 atatcacagc tgatatgatt ttctttgtgc caagacggtt tttgagaatc gagattactt
    39721 cagccagtgt attttcttcg gctttaagag ctggtttgat tattttacct agtacgtagc
    39781 ttctctcgtt tagagatact tggtgtgctc tgccaactgg ttgaatttgc acaataccga
    39841 acttttctag ttcatccaat gtttttgata cttccggatg agatgcgcca gcatcttctg
    39901 ccagccttcg gatggtatag atcttaccct tgtacctaac cagagtacgg agtaccctaa
    39961 ccgcgatcct gtttccaagt atttgttcta gatagttgtg taatttcatg caatacccct
    40021 cttccatatg gaaggaatat ttaccatatg gtaagtaaat ctaccatata actttattga
    40081 taaattttaa attcgtggtc atctagagtt cagcaattag ttgcatttgt ataattcacg
    40141 ctaaataatg actaatcttt taaaagattt caaattaagg ataatttgga atttgattcg
    40201 aaattttatt tttaaagggc cctcgagggg aatcgaaccc ctgtccgggg atccacagtc
    40261 ccctatacta gccactgtac tacgaaggcc acaaaggagc catgatttta aagcaagtat
    40321 taacttaacg tatcgatttg gcaaaaaaag atcagaacta agtttattat tctctacaca
    40381 aaaaccccaa taatgcaaag taagggttgg tttattgcca tggccattac aatagccctg
    40441 gtttggtggg gagcaaacta cctatttccg tggcaacact agtgaaatta ggcgtaatgc
    40501 tagtcgacta gccacttttt attgtaaaag tacaggaaca tcaaaagtga cggaataaac
    40561 gaaaatatca tcatgattat gagagtcgta tacggtccaa aaaagtccca gctgccagtc
    40621 tgaattccac cgggcagatt tacgttcatg ccatatagtg ccccaactat tgttggtggg
    40681 atcgtgagtg tgaatattat ggtaagtgtt gcaagaattt tgtttgactt ttccgtgctt
    40741 agcataaagt ccgtatcttt gtagatttcc attgtttctt ttgactcttc aagtgtttca
    40801 actaccttgt caatgtgatc aattacgtca tcaaagtgta tgactagatc atcgtctgga
    40861 tttgaaaatc gctgggtgtt tttcgatgtc tccagcacta tctttcgtaa cgggtttacg
    40921 attctccgaa gtattgtgat ttcccttctt agaagcgaga tcttttttgg agtggatttg
    40981 ttttcatcaa atacatcatc ttctatttca tcaagatttg caatgattct tcgcaacgtg
    41041 tgcaaaagat cgtcaactag tgcatcaatt attttgtaaa gaagaaatcc tgatgacttg
    41101 ccaagcaact cgcctcggag ctgtgcattt tccttgcagt gggcaaacat atcaactagt
    41161 ggtttgagct cgccatagtg gatagttaca agatagtctt ttccaataaa gactgcaagc
    41221 tggctgaatt ttggcattcc cctttttaga actagtggag gaaagtggag gatgataaaa
    41281 gaatgatcat catagttgtc tattttggag agctgaatct ttgatatcga gtcgtccaga
    41341 tttagtgtat tgaaattgta aatttgcccc agtttttcaa tttctgccct gtcaggattt
    41401 tgaatgtcca tccaagaaaa tccgtttcct tggattgtct ccacgtgact ttgtatgact
    41461 tcgtctgcct gtgggtgcct tctaagatgc ggaagtcttg gggtgaagat tacctttttc
    41521 aaatcagcta ccatgccttt ggtaattaat ttaaattttg attatgctat cgcaaacaaa
    41581 cattttacca attggtgtaa tttgaggcta gttaccaaaa catcaagaaa aggaaaaaca
    41641 aatggcgtgg ccccaagcgt agaacgctga gagaacacct ctcactttcc aaacgctaat
    41701 gcgtctatgg atcaccaaac tgttctactg agccacgcgc ggtctatttg ttaggaatcg
    41761 cttccttccg catcagtcct tataggaatt tatgatattt aagatttaga tgtaaaattt
    41821 ttgcataaac tcagttgaat gtgaaaattt tagtccatac gatccaaaca aagatttcat
    41881 atctgatgcc ggaccaccaa ttctgatgag ttgttctgga gaggtcatag aaccagaaaa
    41941 tgagctgatt cagatcaggc ctaacatctt aaagcagcta aaaaaggcca agtacggcgt
    42001 agcagatcat gccacagttg agctgtgtca ttggacaaaa aaatccttca aaaacggcgg
    42061 cgactgctac aagcacaagt tctatggaat ctcgactcac cagtgcatgg agttctcacc
    42121 tgccgggatg cattgtgaaa acaggtgcgt ttactgctgg cgcccaatgg agttttatga
    42181 ttcactaaaa atggatccac gcaaagttgc cgagcctgca gagatgatgg aaaagctgat
    42241 ggaggagcga agaaacctaa tcatgggtca ttacggtgat ccaaaatccg acagacaaaa
    42301 gcttgacgag tcactgctgc caagccatta tgcaatctca ctttctggcg agccgacaat
    42361 gtacccaaag cttccccagc tgataaaata tctaaaagat ttgaagacga caaagtcaat
    42421 ctttttggta acaaacggcc aagagccaga gatgttgcaa aggctacaag acgagaatgc
    42481 cctgccaaca cagatctacc tttcaacaaa tgccgcagac tatgaatcat ttatcaaaat
    42541 aaacaagcca aaatacaagg attcttggga gcgatggaac aagtcccttg agcttctctc
    42601 aaaactggac acaaggactg ttcttaggat cacattaatc agagaataca atgacggtga
    42661 gaaaataatt ccggcatttg cggaaatgct aagaagagca aacgtgcact ttatcgaggt
    42721 aaagtcctac atgcatattg gccgctcaac taatagattg gagcgggaca acatgctaga
    42781 gtttgacgag gtaaggcatt ttgcgttcga gcttgcaaga caaagtggga tttactccat
    42841 catggatgaa agtgatgcgt caagaatcgt cgttttgcaa aaccagcaaa gattcaccga
    42901 acgctggatt gcagcttatt tgaataccaa gtagcgaatt tcacatctga ttttcaatta
    42961 tagcctcaat ttctttgagc ctgtttggat ttaatgagta ttggtgaccg tagcttgtag
    43021 gatgaaacgt tatcaggcca gatttgacta gttttttaat tgccttgtca accattcctt
    43081 tttcatgaga tgcaaagctc ctcttgagcg attcagttga tgtgtgcctt ccaccaacat
    43141 acccataatg atacatttta cttagcacac ggatagcaag agcatccaac tgcattgtac
    43201 attatagcta aaaccgatat ttatctatta tgtattaaaa taccgtatat caggaatata
    43261 tttatacaat agatgtatca agtaaaacat gatgaaacag aaaacaaaac gagctgcgtt
    43321 atctgagaaa aaatcatacg agattggacc gttgacaagt gttttcccat gcagtgagtc
    43381 aaagattcta gatcacatgg tgacaatgag aggtttggat tattctattt ccgatatttc
    43441 aagaatttct ggtgtcaatt tcaaaacaac actcaacata atccacaagc tggaagggca
    43501 ggaagtaatt aaaaagacaa gaacagtcgg caaagcaata atgtacatac taaacgaaaa
    43561 ttcatctcaa gcaaagtcaa ttatcaagct tgcctttgag atagcaaaaa agcgaaataa
    43621 tgagattcta gctaaacgct aatgaatctt tgaataccaa gtagtaaatt tttgcaaagc
    43681 cctatacctg tgcgatatgg tgtttttgtc gctcatttct gcgtaggttt tgtttttgcc
    43741 atttggaaca aagattgggt catagcccca gcctttgcct tttggctttg cagaaatccg
    43801 cccccttatt ctgccctcaa aaagcatcgg tcttcttcga ttatcacaaa atgcgattac
    43861 agagacaaag tccgcgtttt ttttcgtttt taccaaattt atgattccgg cattgccaat
    43921 tgtcttaaaa acatacgatg agtacgggcc tggaaatcct cccagcgaat cgatgaaaag
    43981 gccgtcgtct tcaacaatta ctgcagatct gcattttttg tttgcatcga gcgccttttg
    44041 ttttgcaatt tgcccaatgg aatgggattg gatctcaact gcggagaatt tcaaaaaccc
    44101 aagccttatg tcaaattgcg ataagatttc ttttgcttcc ctgtacttgt ttttattaga
    44161 tgatgcaaaa accaagtcat acgacttgcg catatcttcc cctgccctca attactgaaa
    44221 cgtagtttag aaccttattg aatctctcag tttccaccac tgacttgtat cctgccaaaa
    44281 agcttggcca gaatacgttc atcatatcaa cgtgtgcgct gtttagaatt tccttgaaga
    44341 gtcttagatc cactgcatgg tcttcgactt tagaagtttt ttgcgccagc ccgaaatcga
    44401 aaatgtatgt tttatttttg cttacaacaa agttggatgt tgtaagatca ccgtgcatta
    44461 tcccgttttt gtgcagcgtt cctgtgattt tgcctatttc tttgcaggca tttatcagct
    44521 ttgagccttt taggtttcgt gccagatccc ctttgatgtg ttgcatcaaa atggtacagt
    44581 catttgcgtt tacttggtag actagtggtg tgcgaactcc aaataatttt gattcagcaa
    44641 gcagacttgc ttcccgtatt gtgcgttgcc ttctaatttt gtcatcgagt atagtgttgc
    44701 ggtatggttt tttcttgcgc gtcttga
//
LOCUS       DASWKH010000030        17428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3389711, whole genome shotgun sequence.
ACCESSION   DASWKH010000030 DASWKH010000000
VERSION     DASWKH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3389711"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VGA92_02355"
     CDS             <1..389
                     /locus_tag="VGA92_02355"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9845287.1"
                     /translation="IIREGNIVNIGSVDALGTPNISPRYVMAILDDERLIFADAYMNK
                     TYANVKLWPKVTAAIVDKANRAGFQLKGDAEEITDPDLIKQCANKLKELGFESGPVKV
                     WALDVKEIYSIKPSESSKLPLMSAYG"
     gene            519..1106
                     /locus_tag="VGA92_02360"
     CDS             519..1106
                     /locus_tag="VGA92_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845288.1"
                     /translation="MVNALEQQISNFIDKSFVIQEGNQTVSDGVKKMEEYGVDSLVII
                     NNNDINGMVTHRDVLFDVVAKGKDPTRITLKEIMRTPLVAIQKDAKVKDAISMMNKNN
                     VRRLVVLDNKIPIGMVSQKVLVGNIAKQAIVLPELEIPNKIKCPYCSSIFDNKGALSS
                     HIDNIHIGRGLFEGNLARVDELGSINPSSDFPKTL"
     gene            1212..1676
                     /locus_tag="VGA92_02365"
     CDS             1212..1676
                     /locus_tag="VGA92_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9845289.1"
                     /translation="MTYKTILVPHDGSKAADKALSHAIQIAKLSNAKIVIVNVVTEIL
                     YPNYISFRGAAVNVKSARDLAKEIYYEMKKIAQKLLDSKKKQCEDNGVSVEMVVSYGN
                     VSGSILDLIKSKNVDLVVMGTTGLSGISKIKVLGSVARNISEKSPCPVLLVH"
     gene            1782..3200
                     /locus_tag="VGA92_02370"
     CDS             1782..3200
                     /locus_tag="VGA92_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX9845290.1"
                     /translation="MQTKRRFNIGSTGFVLGPILFFAIILAPFGELSFEAKAVLGLAL
                     WMGTWWVTEAIPIYVTALLPLVLFPLLNVTDFGKTSSFYADRIVFLLLGGFFLAATIE
                     RSNLHQRFAFTMLKFSGTNPKYIVGAFVIVTGILSAWMSNTATTVLMLPIAAAILSSI
                     NIHNKERFGVCLMLCIGYAASIGGVGTLIGTPPNAIFASLSKSLVGIDVSFVQWMLVG
                     IPVSAISLFVMWLYMVNVGVKLKDVTISGEGDFVLKKLQALGKLSKDEKIVALVFAVT
                     ITAWITRGLVWGQFLPFVDDSTIAILAAIAVFLIPSTKNKRLLDWSTATKIPWGILLL
                     IGGGLALAGGFTITGVDEWIASQLSFLKGLHYFVILLVLVLIVVFAEFISNTATAALI
                     IPISAALASVLDINPILLMTPVAIAASFGFILPTSTPPNAIIISSGYVNAKKMARIGL
                     PLNIIAVVLVALLTSILVPILW"
     gene            3204..4160
                     /locus_tag="VGA92_02375"
     CDS             3204..4160
                     /locus_tag="VGA92_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX9845291.1"
                     /translation="MSELIIQILILGGFIALLVKSAIFAIESIVKFSKITGIGELAAG
                     FVIVAVATSTPEISVAFFSTASENIGITLGDIFGSNVTNIALISAIFLLISPIKKIEH
                     KTLRTMLPLLLASSAIPLLLLMTQDGTKFIGVALLAVFGFFLYYVFKFHPSEQKERVG
                     GSAIKPLVYFFIGISLVIVSSKFIVDSASFLAESTGIRESVIGATVVAIGTSLPELTI
                     DIVAVKRRHLDLALGDIVGSCISNITLVLGIVLVLSSVSINFGILSSLIGFAMIAPFA
                     LFVFIRKALVKKWHSFLLFGIYAAFLLTIYEIQLHIGGIKIG"
     gene            complement(4549..5415)
                     /locus_tag="VGA92_02380"
     CDS             complement(4549..5415)
                     /locus_tag="VGA92_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX9845292.1"
                     /translation="MDCKEHLLVNDHESGELVCGRCGLVIQEKIIEERPEWRTLENSS
                     ERSRVGDGTYLSKHDRGLATIIGPANKDVTGKPLSVSMKNTISRLRIWDSRSQAYNSS
                     ARNLRRAFSELLSMREKLGLSDVIVEKTAYLYRKAHEKKLTRGRPISAFICAALYAAC
                     RDSETPRTVKDIAKASGIKSKQINKCYRRIIEGLDLRMPTVNPIQCVSKIANKVGVSE
                     KTKRVAVSILQNYEKSGNSAGKSPMCIAATAVYLAAIQNDENYTQKAIANAANVTEVA
                     IRNRVAEIRMLN"
     gene            5543..5695
                     /locus_tag="VGA92_02385"
     CDS             5543..5695
                     /locus_tag="VGA92_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845293.1"
                     /translation="MSISFVTKLAKSGSASNGKERRIIIIPTDKVKNIVEYYEGKQVK
                     VTIEEI"
     gene            complement(5958..6188)
                     /locus_tag="VGA92_02390"
     CDS             complement(5958..6188)
                     /locus_tag="VGA92_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845294.1"
                     /translation="MQKSQPLCLNDNCYNVVQQLSKTMRFLSHVDQYVADANKTGDKD
                     SEKIWNTIKTDRQKHAELLKNLIISEIRNNKF"
     gene            complement(6290..6433)
                     /locus_tag="VGA92_02395"
     CDS             complement(6290..6433)
                     /locus_tag="VGA92_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845295.1"
                     /translation="MKPKKSKKLEEEKSKKEVEREQMLEQAHQLDLQKNYEYDEKLEE
                     STD"
     gene            6698..7063
                     /locus_tag="VGA92_02400"
     CDS             6698..7063
                     /locus_tag="VGA92_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9845296.1"
                     /translation="MVLCFSTMKRIEAIIQEDKLSIVVDALKTTGVGGVTIMQSRGIG
                     AGERPIIGGARGTSKRVAPFNRLCTILVIIDDAKVSSVVSSIMDAAHTGKAGDGKIFI
                     TNVEEAFDIATKESGKDII"
     gene            7176..7958
                     /locus_tag="VGA92_02405"
     CDS             7176..7958
                     /locus_tag="VGA92_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845297.1"
                     /translation="MSLNITDLKVADYLSPHPITISKKASFADAVSLMTDKGIGNLII
                     KDGHSVEGILTERELLRYLAKEKKIPDEPIQNITASNFTKIPLETTLLIAAKTMLSTK
                     SRILVFDNEKLIGIITASDMLRGFRKTNQNPTLEEVMSKKIYQCSYNDTILKAVKLMH
                     LKRIGSVIITKDKKPYGIFTERDLLVNVLANEVELDGKVGGYCSSPLITARVGIGCKD
                     VSDVMSENKIKRLVLIDDKGMQSIVTARDIVDAFSSYFTPYL"
     gene            complement(8036..9091)
                     /gene="amrS"
                     /locus_tag="VGA92_02410"
     CDS             complement(8036..9091)
                     /gene="amrS"
                     /locus_tag="VGA92_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9845298.1"
                     /translation="MQKEAILYERLPNDKVRCLACARYCEIGKDQIGSCGIRGNKDGK
                     LMLYVYGKVAAVHIDPIEKKPVTHYMPGTKIFSIGTSGCNWYCRFCQNFDLSQRRKIE
                     GQDYSPEELVASALNSKCHGIAYTYNEPTIFIEFARDCGVLARKNGLFNIFVSNGYCT
                     PESVKMMTEFLDCITVDFKGNGDQQFVRRYVGIPNSQPVFDTLLELKKTNIHVEITDL
                     VVPRVGDNLEHAKKMCKFIYDNFGPEMSIHFLRFHPDYKMMEFEPTPVETLEAHYNVA
                     KKEGLKYVYIGNVPGHPYEHTYCSECKKIVVRRYNFDILKWDLDDDNKCKFCGNKIPI
                     TGKLDKDYKERRFEFVT"
     gene            9194..10396
                     /locus_tag="VGA92_02415"
     CDS             9194..10396
                     /locus_tag="VGA92_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9845299.1"
                     /translation="MSYKTFVENTHDTPTFKKQFEIVERKGLGHPDTICDLVMDKVSI
                     ELSKLYIKETGTIQHHNMDKTMLVAGQTENKFGGGRVIKPLKLVIGDRVTILSDDHKV
                     PISDFIITTAKAWFEKNLKNIGEKNIEFQMEIGQSSKELQSIFKNPKSFASNDTSALV
                     GYAPFTATESIVFETEQYINSKKFKDEFPASGEDVKIMGFRNDNWVDLTVAIAFVDKY
                     VESVDDYFRKKHQMLEAINEFAKTKTDLNIHTAINCLDDQNKGMDGIYLTVLGTSADN
                     ADSGEVGRGNRANRVISISRPAGAEALAGKNPVSHIGKIYNSLSFKLADEIYNKVSGI
                     EEVFVWMYNVIGRPVNDPKAVVVRPLTKNAELDKSQQIQITEIVSDNLEKMDEFCKKL
                     LSEEIQIA"
     gene            complement(10398..11852)
                     /locus_tag="VGA92_02420"
     CDS             complement(10398..11852)
                     /locus_tag="VGA92_02420"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9845300.1"
                     /translation="MESIKKVSNLEYRIETDFASGMKVPVRIFANEHLLAKMVLDRTL
                     EQAVNVTMLPGVQKNVVVLPDGHQGYGFPVGGVAIMDYDEGVISPGSVGYDINCGVRL
                     IRTNLTEAQLKPKLKELVTDLFVSIPAGWSRHRSEGCIDLSSSQLDDILVQGVPWLLN
                     NGYATKDDVELCEEYGHMHGADTNKVSATAHKRGEPQLGSLGSGNHFLEVQKIDKIFD
                     KEAAQLMGLYNEGDITVLIHCGSRGFGHQICKDYLEVCERSYQKYGIEIPDRQLACVP
                     NLSEEGESYKKAMKAAMNFAWSNRQTITHWTRKSFERVFGQSEADLDMKLVYDIAHNS
                     AKTETHLVDGMEKKVVVHRKGATRAFPAGRPEIPSKYRSMGQPTFIPGSMGSASWVLL
                     GKANSLELTFGSTVHGAGRLMSRTAAHKTYNYKQVIDQLEEKGIFLKSVTRYDVVEEA
                     PQVYKDVDTVAEISHQLGIATKVARLVPLGVIKG"
     gene            complement(11863..12063)
                     /locus_tag="VGA92_02425"
     CDS             complement(11863..12063)
                     /locus_tag="VGA92_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9845301.1"
                     /translation="MFDDIFASLDLGLGSVWVGSFDEGSVSQILDIPSSLQPIALLVV
                     GFATEKPEITPRKPLSEITHIV"
     gene            12164..12604
                     /locus_tag="VGA92_02430"
     CDS             12164..12604
                     /locus_tag="VGA92_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX9845302.1"
                     /translation="MKIDEQPDEDYEVVKIDKIGVVDQLTGIMILKSMDGKEFHITAF
                     SSEVVGHIMNFIDGQRQTIPTIYNMFEQICEENELVLVSVKIYESGGIFRANLYFTGK
                     KEVVLRNYRASDAITLATFYSVPILVRKNLLKEPEPQPVESSKK"
     gene            complement(12638..12958)
                     /locus_tag="VGA92_02435"
     CDS             complement(12638..12958)
                     /locus_tag="VGA92_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9845303.1"
                     /translation="MQTLAIMDESKKESILEAVSDRYSRIILEATMSKPKTALEISTE
                     YGVPISTVYRRLQALHDARLVGISGSISADGKKYFMYKSKLRSVSTSFDGSKIEISII
                     PNVG"
     gene            13406..13930
                     /locus_tag="VGA92_02440"
     CDS             13406..13930
                     /locus_tag="VGA92_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845304.1"
                     /translation="MTTFQLGYSESVDSIQLVAKLDIAELMMQKYQLNADQRDFTIFY
                     RISTLESVGEGNTEDFDAKVVAMEINQERTSLVITLDNIKKTDLMSVRFPQELISAEK
                     GKFTLLVDGEERGYEWSVQEESRNLIFIIPKQTTEVEIIGNRVIPEFTSGLLVLIIVS
                     STLLITQRLYKKLV"
     gene            complement(13927..14739)
                     /locus_tag="VGA92_02445"
     CDS             complement(13927..14739)
                     /locus_tag="VGA92_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845305.1"
                     /translation="MKISEAAIRDLLPLSLMTTPCVSIEAKNNVQEAVGLLVPYLESM
                     ADSLVVTGDKNQPIGIIGGKEVIEKVLANPSSRIFEASAESIMSPNITKISGATKLRE
                     IIEQWKQTGRAFSIIPNALGGYSAISARKLLEVGKTSMTEMTISELPKKKIVTFKIEA
                     KLNEIINMMLKHKTRKLLLENTNQFVSDRIIIEKIAADLNYLRDSNRFLDLPLVGFNL
                     EYAKVIYKDIKINELASIMFGMMHPYVVFRDQVVSPWDICLSLLSDRFEEYG"
     gene            14873..16486
                     /locus_tag="VGA92_02450"
     CDS             14873..16486
                     /locus_tag="VGA92_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9845306.1"
                     /translation="MSRVLALIPLLALFSFGLVYGQHHGGEAAPPVSFGNKQVTVSTI
                     IEPADFIPGTHPSANLKVRFFDSQTNTNIEKVTYRVQIFYGQSLLASQMFYDSDGELD
                     VKIHPSSECAEKELWRCTKYEGDKDLVVPGALTSSFTSTPIIRGPVFDKSGTYTVKVA
                     IIGATNPKTQTTEDINFETNINIAQEQKFSIKTAQGETPILIKAFQDKLSNFQFSEST
                     KSISFEMPFHWEYAENTLLVRNDIEIPKGFAPFQNINSFKGTINGIPIFPKDIHFDPY
                     SNKDSNVIHFLVTGEELKILKQKIGSDEHTMIVQIMPDTSVAIKSTDVTFSNGYKAAV
                     SYDTRYGASKDVSFTIAFFDSAGNLAKDIRYAYAVKDSTGDEFIVNTGGNTNRLGISL
                     ASGVDSRLITIPSEGSYALQLVLIGKGLIDFDPFVPASMQFDISKIASAAPSTPPTQA
                     AIPNWIKNSAKWWADGTIGDSDFVYGIQFLIKEGIVKIPPTTAGEASSNQIPVWVKKN
                     AKWWADGTIGDSDFISGIQYLIKQGIIRV"
BASE COUNT         5431 a         3408 c         3237 g         5352 t
ORIGIN      
        1 taatcataag agaaggaaac atagtaaata ttggaagcgt tgatgctttg ggtacgccaa
       61 acatctcccc acgatatgtt atggccatac tggatgatga aaggctaatc tttgcagacg
      121 catacatgaa caaaacttat gctaacgtga agctatggcc aaaggtcaca gcggcaatag
      181 ttgacaaggc aaacagagct gggttccaac ttaaaggaga cgctgaagaa attacagatc
      241 cagatttgat taaacaatgc gcaaataaac taaaagaact tggatttgaa tctggacccg
      301 taaaagtctg ggcacttgac gtaaaagaga tctactccat aaagccaagc gagtcatcaa
      361 aacttccact catgtctgca tatgggtaaa agctcaaatc aaagaaaaat ttgctaaaga
      421 atcaattatc ccagctgttt gactgactac tcacattatt attcccacaa ttgataatcg
      481 atcttggatt taaaatgaat gtaaattgag tataatctat ggttaatgcg cttgaacagc
      541 aaatttcaaa tttcatagat aaatcatttg tgattcaaga aggaaaccaa accgtatctg
      601 atggagtcaa gaaaatggaa gaatatggtg ttgatagcct agtaatcata aacaataatg
      661 acataaatgg tatggttacg cacagagacg ttctctttga tgttgtagcc aaaggaaaag
      721 atcctactag gatcacacta aaagaaatta tgcgaacgcc acttgttgcc atacaaaaag
      781 atgccaaagt caaagatgcc atatctatga tgaacaaaaa caatgttcgt aggcttgtcg
      841 ttttggacaa caaaatacca attggtatgg tttctcagaa ggtcttggtt gggaatattg
      901 caaagcaagc aatagtctta ccagaacttg agattccaaa taaaatcaaa tgtccgtact
      961 gttcttcgat atttgataat aaaggtgcac tttcgtcaca catagacaat attcatatcg
     1021 gcagaggcct ctttgagggc aatttagcaa gagttgacga attaggttcc ataaacccat
     1081 catctgattt cccaaagacg ctttaattgt tgcaaaatct ggttttacaa tgaacttgta
     1141 caaaagaaaa cacgatatcg tttttaatat ttagaaattc atttaattac cgtgccttgt
     1201 aattcacata tatgacatac aaaacaatct tagttccaca tgatggttct aaagccgctg
     1261 acaaagcgct atcacatgca attcagatag caaaactatc aaatgcaaaa atagtcatag
     1321 taaatgtggt aactgagatt ctgtacccaa attacatctc atttcgtggg gcagcagtta
     1381 atgtcaaatc tgcaagggat ttagcaaaag aaatctatta cgaaatgaaa aaaattgctc
     1441 aaaaactact tgattcaaaa aagaaacaat gtgaggataa tggagtatct gttgaaatgg
     1501 ttgtttccta tgggaatgta agcggttcta ttcttgatct aataaaaagt aaaaatgttg
     1561 atcttgtcgt aatgggaact acgggcctga gtggaatatc taagatcaaa gtgctgggaa
     1621 gcgttgcaag aaacatctca gaaaaatccc cctgcccagt cctattggtt cactaaatta
     1681 tgaatatgcg ctgattataa caaaagaatt gtcatacggt tacaaatttc agacttgata
     1741 atcgtctttg ttttataatt tgtcattgat acacataaat catgcaaact aaacgacggt
     1801 tcaacatagg aagcacagga tttgtcttag gaccgattct tttctttgca atcattctgg
     1861 ccccgtttgg tgagttatct tttgaggcaa aggccgttct tggcttggcg ttgtggatgg
     1921 gaacctggtg ggtaactgaa gcgattccaa tttacgttac tgcacttttg cccttggtac
     1981 tttttccgct actaaatgtg actgattttg gaaaaacatc ctcgttttat gcagacagaa
     2041 tagtcttttt gcttttgggg ggattttttt tggctgcaac aattgagagg agcaacctgc
     2101 accagaggtt tgcatttacg atgctcaagt tttctggtac taatccaaaa tacattgtag
     2161 gtgcgtttgt aatagtaact ggaatcctta gcgcatggat gagtaacact gcaacgacgg
     2221 ttctaatgct tccaattgct gctgctattc ttagctcaat taacatacac aacaaagagc
     2281 gattcggtgt ttgcttgatg ctctgcatcg gatatgcagc aagcataggg ggggttggaa
     2341 ccctaattgg aacccccccc aatgcaattt ttgcctcact ctcaaaatcg cttgttggca
     2401 tagatgtgag ctttgtacag tggatgttag ttggcatccc agttagtgct atctcgcttt
     2461 ttgtaatgtg gctttacatg gtaaatgtcg gagtcaaatt aaaagacgta acaatttctg
     2521 gggaaggcga ctttgtttta aaaaagcttc aagctcttgg caagctaagc aaggatgaaa
     2581 aaattgtagc acttgttttt gctgtaacca taactgcctg gataactagg ggcctagttt
     2641 ggggtcaatt cttaccattt gttgacgatt ccacaattgc aattttggca gcgattgctg
     2701 tgtttcttat cccctctaca aaaaacaaaa gactccttga ctggagtact gcaacaaaaa
     2761 tcccttgggg catcttgtta ctaattggag gtggattggc gcttgcaggg ggatttacca
     2821 ttaccggcgt tgatgagtgg attgcatcac agctttcatt tcttaagggt ttacattatt
     2881 ttgtaatact tctcgtattg gtattaatcg tggtatttgc tgaatttatc agcaacactg
     2941 ctactgcagc cctaataatt ccaatctcag cagcacttgc atctgttttg gacataaacc
     3001 caatactgct tatgacgcct gttgccattg ctgccagctt tggattcata ctaccaacga
     3061 gcacgcctcc taacgcaatc ataatttcaa gcggttatgt gaatgcaaag aaaatggcca
     3121 gaatcggatt gcccctaaat attattgcag tagtactagt agctttattg acgtcaatac
     3181 ttgtgcctat tttgtggtag gaattgtcag aactaataat ccagatacta atacttggtg
     3241 gctttatcgc actccttgta aagagtgcaa tttttgcaat cgagtcgata gtcaagttct
     3301 caaaaattac aggcattggc gagcttgcag caggatttgt tatagtggca gttgccacat
     3361 ctactcctga gattagtgtt gcattttttt caactgcaag cgaaaacatt ggaataacac
     3421 tcggagatat atttggatca aatgttacca acattgcgtt aatttctgcc atattcttac
     3481 tgatatcacc aattaaaaaa attgagcaca agacactacg aacaatgctt ccactacttt
     3541 tggcatcttc tgcaattccc ttactcttgt tgatgacgca agatggcacc aagtttatcg
     3601 gtgtggcact tcttgccgtc tttggattct ttttgtacta tgtattcaag tttcatccat
     3661 ctgaacaaaa agaacgagtg gggggctctg caataaaacc tctagtttac ttttttatcg
     3721 gcatatcact tgtaatagtt agttcaaaat tcattgtgga tagtgcttcc tttcttgcgg
     3781 aatccactgg aataagagag tctgtaattg gagctacggt agtggcaata ggcacctcgc
     3841 ttcctgagct gaccatagat attgttgctg tcaagcgtcg acaccttgat ttggcccttg
     3901 gcgatattgt gggctcttgc atttccaaca taactttggt actgggaatt gtacttgtcc
     3961 tttcatcagt cagcatcaat tttggaatac tatcatcgct gattggattt gcgatgattg
     4021 ctccatttgc cttgtttgtg ttcatcagga aggctttggt aaaaaaatgg catagctttc
     4081 ttctttttgg aatttacgca gcatttctgc taacaatata tgaaattcag ctacacattg
     4141 gcggaattaa aattggctaa attttgtaaa gaagcgaatc gtagttaaat tggctttata
     4201 ttatagtcgt ccaatagaag ctaagaactt acaaaagtcc atgacatctt gactagaaat
     4261 tgaatttctt tcaattttat ggctaatttc gttaactata cccaaccacc ctgtcatcta
     4321 ttccttccaa aagcaccctg atgatcgcta aggcaacact ggcattcctt tttgtaacat
     4381 tcttgtactg actcatgacc acaggcgcta caatcacaac gcttttcttc catctatttt
     4441 actagccata taatttctat taaagcaggc tgtgattatc aaagttgaat gtatatcttt
     4501 caaaacgtgc tcacatttga gagtgtgtca cagcagaaat gctttctact aatttaacat
     4561 tcttatctct gctaccctgt ttctaattgc tacttctgtt acgtttgcgg cattggcaat
     4621 cgctttttgt gtataatttt cgtcattttg tattgcagca agataaactg ccgttgcagc
     4681 aatgcacatt gggcttttgc cagccgagtt tccacttttt tcataattct ggaggattga
     4741 aactgcaact cgttttgttt tctcagacac acccacttta ttggctattt ttgaaacaca
     4801 ttgtatggga ttcacagtgg gcattctcaa atctagtcct tctattattc gacgataaca
     4861 tttgttgatc tgttttgatt ttataccact tgccttcgct atatccttta cagtcctggg
     4921 cgtttcacta tctctacatg ctgcatatag agctgcgcaa ataaatgcag aaatcggtct
     4981 tcctctagtt agtttttttt catgggcttt tcgataaagg taagctgttt tttctacaat
     5041 aacatctgat aatccaagct tttctctcat tgacagtaat tcgctaaacg cccgtcgtag
     5101 gtttcttgca ctagaattgt atgcctgact tcgactgtcc catattctca gtcgagatat
     5161 ggtatttttc atacttactg aaagtggttt tcctgttacg tccttgtttg caggaccaat
     5221 tattgttgct aaacctctat catgtttgct aagataggtg ccatcaccta ctctgcttct
     5281 ttcagaagaa ttctcaaggg tacgccattc cggtctttct tcaattattt tttcttgtat
     5341 tactagacca catctgccgc agactagctc cccactttcg tggtcattga ctaagaggtg
     5401 ttccttacaa tccatggtat ttactacatc ctgattttgc ttcaataaca tacataatta
     5461 tctacaatta tacatcaccc cacatattta aatattgtgt ataattatac ataattatat
     5521 acaggagctt gatacaatta tcatgtcaat atcgtttgta acaaagcttg caaagtctgg
     5581 aagtgcctca aacggtaaag aaagaagaat catcataatt cctacggaca aagtaaaaaa
     5641 catcgtcgaa tactacgaag gcaaacaagt caaagttaca atagaagaga tatagtaact
     5701 tgaaattcat taatgagtca agtgagcaat agtcttcttt gcttagatta atccgaagac
     5761 caatcagttg atttgatcgc tcaatcagct ttcatcaaag ttggctatta cccaagatat
     5821 aatgctcaac atcatattag aacttgatca agattttcag gtctgatact aggatctaaa
     5881 aattaatttt ttggaattta atttataatt taatgcgaaa ctgtgattat ttttagtata
     5941 atttaaaaaa tatgggatta gaatttgtta tttctaatct cactgattat tagatttttt
     6001 aacaattctg cgtgtttttg tctgtccgtc ttgatcgtgt tccagatttt ctctgagtct
     6061 ttgtctcctg ttttgtttgc atcagctaca tactgatcaa catgagacaa gaatctcatt
     6121 gtttttgata gctgttgaac aacattgtag cagttatcat ttaggcatag gggttgtgat
     6181 ttttgcatta ttaaataact gattttttaa tattagaatt atacagttga ttatcaagaa
     6241 tgatactttc tacccaaaat ccaccatttt ggtgcagatc gaagtacgat cagtcagttg
     6301 actcttcaag tttttcgtca tattcgtaat ttttctgtag atcgagttga tgtgcctgtt
     6361 caagcatctg ttctcgttca acttcttttt tgctcttttc ttcttcaagt ttttttgact
     6421 ttttaggctt catacatgta atgcgtggta actaaattat gaaaagtacc tagattttca
     6481 taaatgaaaa ttatcactta tcttttatta aatagtcctc ttctcattaa cagggtaatg
     6541 acactcgaga gaaaatgcgt gcctttacgg taatgcgttt ttaagaagac gagtaactaa
     6601 aggattacca cgctctgaat cctgggaggt tgttaccctt tttagattat gaaatatgtc
     6661 aaaatcttat ttgataatca tctaatcatg ttttaaaatg gtattgtgtt tttctaccat
     6721 gaaaagaata gaggcgatta tacaagaaga taagcttagt atcgttgtag acgccttaaa
     6781 gactaccggt gttggcgggg tcacaatcat gcaatcccgt ggtattggag caggtgaaag
     6841 gccaataatt ggcggtgcca ggggaacttc aaaacgtgtg gcgccattta atagactatg
     6901 cacgatttta gtaataatag atgacgctaa ggtcagctca gttgtttcat ccattatgga
     6961 tgctgcacat acgggcaaag ctggcgacgg caagatcttc ataacaaatg tagaagaagc
     7021 ctttgacatc gcaacaaaag aaagtggaaa agacatcatc tgagattcat tttttataat
     7081 atggaattat aatgaatggg actaaaccta ggatgattat cacctctgag atccgacatt
     7141 cataaacaaa tagtgttatg acgtaataca acaaaatgag cttaaacata acggatttga
     7201 aagtagcaga ttacttgtca ccacatccaa taaccataag caaaaaagct tcatttgccg
     7261 acgcagtgtc gcttatgacc gataaaggaa ttggcaattt aataataaaa gacggtcata
     7321 gtgttgaagg aatcctcaca gaaagagagc ttttacgtta tttggcaaag gaaaaaaaga
     7381 tacctgacga accaatccaa aatattactg ccagcaattt taccaaaata cctttagaaa
     7441 caactcttct tattgcagca aaaacaatgc tttctacaaa atcaaggata ctcgtatttg
     7501 acaatgaaaa actaattgga ataattactg cctctgatat gcttagagga tttagaaaaa
     7561 caaaccaaaa tcctactttg gaagaggtaa tgagtaaaaa gatttaccag tgttcataca
     7621 atgatactat tctaaaagct gtaaaactta tgcacctaaa gaggatagga agtgtgatta
     7681 ttacaaagga caaaaagcca tacgggattt ttaccgaacg agatcttctt gtgaatgtgc
     7741 ttgcaaatga ggttgaatta gatggaaaag tgggtggtta ctgttcatct ccattaatca
     7801 ctgcaagggt gggaataggc tgtaaagatg tttcagacgt aatgtcagaa aacaagataa
     7861 agcgattggt gttaattgat gataaaggaa tgcaatctat tgtgacagca agagacatag
     7921 ttgacgcatt ttcaagttat tttacgcctt acctataact actgatatta gttcacacaa
     7981 tcacgcctcg aatttgacgt cttaaactca ttttgacaaa aatgaggttt taaaactagg
     8041 tgacaaactc aaaccgtctt tctttgtaat ctttgtctaa ctttccagtt attgggattt
     8101 tgtttccaca aaacttacat ttgttatcgt catctaaatc ccactttaga atgtcaaaat
     8161 tgtatcttct tacaactatt tttttgcatt cggaacagta agtgtgctcg tatggatgtc
     8221 caggtacgtt tccaatatac acatacttca aaccttcttt ttttgcaaca ttataatgag
     8281 cttctagcgt ctctacaggc gttggctcaa actccatcat cttgtagtct ggatgaaatc
     8341 tcaaaaaatg aatcgacatc tctggaccaa aattatcata aataaattta cacatttttt
     8401 ttgcgtgttc taggttgtcc cccaccctag gtacgacaag atctgtaatc tctacatgta
     8461 tgttggtctt tttcaactca agcaacgtat caaagactgg ctgcgagttt ggaatcccaa
     8521 catagcgtct gacaaattgt tgatctccat ttcctttaaa gtcaactgta atacaatcaa
     8581 gaaactctgt catcattttt acagattctg gagtgcaata cccatttgag acaaaaatgt
     8641 taaacaaacc attctttctt gccaaaacac cacagtctct tgcaaattcg ataaagattg
     8701 ttggctcatt gtaggtatag gcaattccat ggcacttgga gtttaaggcg cttgcaacaa
     8761 gctcctctgg agaatagtct tgaccctcga tctttcttct ttggctaaga tcaaagttct
     8821 ggcaaaatct gcaataccaa ttacaaccac tcgtaccaat agagaagatc tttgtgccag
     8881 gcatataatg tgtaactggt tttttctcaa tagggtctat gtgtactgca gcaaccttgc
     8941 catatacgta aagcattagc ttaccgtcct tgtttccacg aattccacac gacccaattt
     9001 ggtccttgcc aatctcacaa taccttgcgc acgcaagaca tctcacctta tcatttggca
     9061 acctttcgta caggattgct tctttttgca ttagcattga tttgcttttg tttgatatta
     9121 atgtggaaac caattatcaa gtctggaaat tatattttaa gctaatttag gataaatccc
     9181 atttcaaagt ataatgtcat acaaaacttt tgtagaaaat acacacgata ctcccacatt
     9241 taaaaaacaa tttgaaattg ttgaaagaaa aggacttgga cacccagaca caatttgtga
     9301 tttagtcatg gacaaagttt caatcgaatt atcaaagctg tacataaaag aaactggaac
     9361 catacagcac cacaacatgg acaaaacgat gcttgtggca ggtcagacag aaaacaagtt
     9421 tggcggaggt cgcgtcataa aaccactcaa actcgtcata ggtgatagag tcacaatcct
     9481 ctcagacgat cacaaagttc caattagtga tttcataata acaaccgcaa aagcttggtt
     9541 tgaaaaaaat ctaaaaaaca ttggagaaaa aaacatcgag tttcagatgg agataggtca
     9601 gagctcaaaa gaacttcaat caatcttcaa aaatccaaaa tcgtttgctt caaatgatac
     9661 atcggcactt gttggatatg ctccatttac tgcaacggaa tcgattgttt ttgaaacaga
     9721 gcaatacatt aactcaaaaa agttcaagga tgaattccca gcttcgggcg aagatgtcaa
     9781 gataatgggt tttagaaatg acaattgggt tgatcttact gtggcaattg cttttgttga
     9841 caaatatgta gagtcagttg atgactattt tagaaaaaaa caccagatgc tcgaagcaat
     9901 aaacgaattt gcgaaaacaa agacggatct aaacattcac acggccataa attgtcttga
     9961 tgatcaaaat aaaggaatgg atggaatcta tcttaccgtg cttggaacat ctgcagacaa
    10021 cgctgattca ggcgaggttg gaaggggcaa tagggccaac cgagtgattt ctattagtag
    10081 gcctgctggt gccgaagcac ttgctggaaa aaacccagtc agccatattg gaaaaatcta
    10141 caactcgtta tccttcaagc ttgcggatga aatctataac aaggtgtctg ggattgaaga
    10201 ggtttttgtt tggatgtata acgtgattgg aagaccggta aatgacccaa aggcagtggt
    10261 agtaagacca ttaacaaaaa atgctgaact tgataaatca caacaaatcc agatcacaga
    10321 aatagtaagt gacaaccttg aaaaaatgga cgagttttgt aaaaaattac tttcagaaga
    10381 aatccaaatt gcctagccta tcctttaatg acgcctaaag gaacaagtct tgcaaccttg
    10441 gttgcaattc cgagctggtg tgagatttcc gcaacagtat ctacgtcttt gtagacttgg
    10501 ggtgcttcct ctaccacgtc atatcttgta accgatttca aaaagatccc tttttcttca
    10561 agctgatcaa tcacttgttt atagttgtag gttttatggg cggcagtcct tgacatgagt
    10621 cttcctgccc catgaactgt ggaaccaaaa gttagctcaa gtgagtttgc ctttcctaat
    10681 agaacccagc ttgccgaccc catagaacca ggaatgaatg tcggctgacc catacttctg
    10741 tacttggagg gaatttcagg ccttcctgca ggaaatgcgc gtgttgctcc ttttctatga
    10801 acaactactt ttttctccat tccgtcgact agatgagttt cggtctttgc actgttgtgt
    10861 gcaatatcat aaaccagttt catgtccaaa tccgcctctg attgaccaaa aacacgttca
    10921 aaggattttc ttgtccaatg agtgatggtc tgtctgttgc tccaagcaaa attcattgcg
    10981 gctttcattg cctttttgta cgactccccc tcttcagaaa gatttggaac acacgcaagc
    11041 tgtctgtctg gaatctcaat tccgtacttt tggtagctac gctcacaaac ttcaaggtaa
    11101 tctttgcaga tttggtgccc aaaacccctt gaaccacaat gaatcaaaac tgtaatgtct
    11161 ccctcattgt acaaacccat caactgagct gcttccttat cgaatatctt gtcgattttt
    11221 tgaacttcaa gaaaatgatt tccagaacca agacttccaa gctgcggctc gcccctttta
    11281 tgcgccgttg ctgagacttt gtttgtatca gcaccatgca tatgaccata ttcctcacac
    11341 agttccacat catcttttgt ggcatagcca ttattgagaa gccaaggaac accttgcaca
    11401 agaatatcgt caagctgcga actagataga tctatacaac cctcagacct atgcctactc
    11461 catcctgcag gtattgatac aaaaaggtct gtcacaagct cctttagctt tggttttagc
    11521 tgcgcttcag taagattggt tctaatcaac ctaacaccgc aattgatatc ataaccaaca
    11581 cttccagggc tgatgactcc ttcatcataa tccattatcg caaccccgcc tacgggaaaa
    11641 ccataaccct gatgaccatc tggcaagact acgacatttt tttgaacccc aggtaacatt
    11701 gtaacattaa cagcctgttc aagggttctg tccaaaacca tttttgcaag caagtgttca
    11761 tttgcaaaaa ttctgactgg aaccttcatt ccacttgcaa aatctgtctc aattcggtat
    11821 tctaggttgc taaccttctt gatactttcc atcattacaa attcacacaa tatgtgtaat
    11881 ttcacttaga ggttttcttg gagtaatttc tggtttttct gtggcaaaac ctactacaag
    11941 caaagcaatt ggttgcaatg atgagggaat atcaagaatc tgtgagactg atccctcatc
    12001 aaaggaacct acccacactg aaccaagacc caagtcaaga ctagcaaaga tgtcatcaaa
    12061 cattactggt ttgttatgat tttggataaa ataagatgtg ctccattttc agtaatgaaa
    12121 atcataaagt caattttatc tatgaaaaac tacattattg ataatgaaaa ttgatgaaca
    12181 accagacgaa gactatgaag tagtcaaaat agacaagatt ggggtggtag accaactcac
    12241 aggcataatg attctaaaat caatggatgg aaaagaattc cacataactg cgttttcatc
    12301 tgaggtagtg ggccacatca tgaatttcat agatgggcaa cgtcaaacaa ttccaacaat
    12361 ttacaacatg tttgagcaaa tctgtgaaga aaatgaactt gttttagtaa gtgtcaaaat
    12421 ttatgaaagt ggaggaattt tcagagccaa tctgtatttt actggcaaaa aagaggttgt
    12481 tttaagaaac tacagggcat ctgatgcaat cacacttgcc acattctaca gtgtacctat
    12541 cttggttaga aaaaatctac taaaagagcc agagccacaa ccagtggaaa gttctaaaaa
    12601 atagatctct gaaataaaaa aaataaaaat atctcaatta accaacattt ggaataattg
    12661 agatctctat tttgcttcca tcaaaggatg ttgaaaccga ccttagcttg cttttgtaca
    12721 taaagtactt ttttccatca gcactaatag aaccagagat tcctacaaga cgagcatcgt
    12781 gcaaggcctg caatcttcta tagactgtag agattggaac gccatattcc gtgcttatct
    12841 ccagtgcagt tttaggcttg ctcatggtgg cttcaagtat tattctggaa tatctgtctg
    12901 aaacagcttc caaaatagac tccttttttg attcgtccat tatagcaaga gtttgcatga
    12961 taagaggatg gattcttttg atataacgag taggactgca ctatagtcta atgacagaca
    13021 gtgcaagaca gtgcactatc taacgcagag gtttcttcta caggcaaggc actttccatc
    13081 cttgaactct gctttgaata tacatttctt acatacactg caacagcaaa gaagttcagc
    13141 tttcatttca atcgctatat ggtgatcttt tctaataaac ggtcagcact aaaatcataa
    13201 ctgaaaaaca atatgtcgtt ttgcagtgca ttgcactaaa agaaatgaat ttgttcaact
    13261 ttactcacga tgtacggaat gctgatgttg ataatgaaca agcattttcg attttggaaa
    13321 tcgacaattt gttcaaataa acaaatcaca tcaatttatc gtgaaaacac aaattatcta
    13381 tgtttttttc tttttatttt taattttgac aacctttcaa ttaggatatt cagaatcagt
    13441 agattccata caattggtag caaaactgga tattgcagaa ttgatgatgc aaaaatacca
    13501 actaaatgct gaccaacgag attttaccat attttataga ataagcacgc ttgaatctgt
    13561 aggagaaggt aacacagagg attttgatgc caaagttgta gccatggaga taaatcaaga
    13621 acgaacgtcc ctcgtaatta cactcgataa cattaagaaa actgatctga tgtctgttag
    13681 attcccacag gagctcatat ctgcagaaaa gggaaaattc acactacttg tcgatgggga
    13741 ggaacggggt tatgagtggt cagtacagga agaatctcgc aacttgattt tcattattcc
    13801 aaaacaaaca acagaagttg aaataattgg aaacagagtc atacctgaat ttacctcggg
    13861 gttactggta ttaattatcg tctcttcaac attactaatt acacaaaggc tttacaagaa
    13921 actggtctaa ccatattcct caaatctgtc agaaagcaaa gacagacaaa tatcccaagg
    13981 acttactacc tgatctctga agaccacata cggatgcatc attccaaaca ttatgcttgc
    14041 aagttcgttt atcttgatgt ctttatagat tactttggca tattccaagt tgaatccaac
    14101 aagtggtaga tcaagaaatc tatttgaatc acgcaggtag ttgagatctg ctgcaatctt
    14161 ttctatgatt attcggtcgc tgacgaactg atttgtattt tctaaaagca gttttcgtgt
    14221 tttgtgcttt agcatcatat taataatctc attaagtttt gcttctatct taaatgtcac
    14281 aatcttcttt ttgggcagct cagagattgt catctctgtc atagatgtct tgcccacttc
    14341 aagcaatttt ctggcagaaa ttgcggaata gccacctaac gcatttggta taatggaaaa
    14401 cgcccttcca gtctgcttcc attgttctat gatttctctt agtttagttg ccccggaaat
    14461 cttagttatg tttggtgaca ttatgctttc agcacttgcc tcaaaaatcc ttgaagaagg
    14521 atttgccagc accttttcta tgacttcttt tccacctatg atcccaattg gctgattttt
    14581 atcacctgtg acaacaagcg aatcagccat tgactccaaa tatggcacaa gaaggccaac
    14641 tgcttcttgc acattgtttt ttgcttctat gcttacgcaa ggcgttgtca ttagcgataa
    14701 tggcaatagg tccctgattg cagcctcgga aatcttcatt tacaaacgtc ttatctatac
    14761 agtggtttta attgggtatt tcgattatca agtctgagaa ttttcaatat tttgcctcat
    14821 tttcggataa atcataaaat aattaataat gccacaaaat gggagaagat cgatgtctag
    14881 agtcttagca ctcattcctt tgcttgcctt gttttcattt gggttagttt atgggcagca
    14941 tcatgggggc gaggctgcac cgccagtaag ctttggcaac aagcaggtca cagtctcaac
    15001 aataatcgag cctgcagact ttattccagg aacgcacccc agcgcaaacc taaaggtgcg
    15061 attttttgac agccagacaa acacaaacat cgaaaaggta acctatcggg ttcagatatt
    15121 ttatggccaa agccttcttg catcccaaat gttttacgac agtgacggcg aacttgatgt
    15181 caagatccat ccaagctcag agtgcgcaga aaaggaactc tggcgctgca ccaaatatga
    15241 gggcgacaag gatttagtcg tgccaggtgc gctcacgtcg tcttttacta gcacgccaat
    15301 aattagaggt ccagtctttg acaagagcgg tacatacaca gtcaaagttg ccataattgg
    15361 agcaacaaac ccaaagaccc agactaccga agacatcaac tttgaaacta acataaacat
    15421 agctcaggag caaaaattct caattaagac tgcacagggc gaaacaccca ttcttatcaa
    15481 ggcgtttcag gacaaactct caaatttcca gtttagcgaa tcaacaaaat caatttcatt
    15541 tgagatgccg tttcattggg aatacgcaga gaatacattg cttgtaagaa atgacatcga
    15601 gattccaaag ggatttgctc ccttccagaa cattaacagc ttcaaaggta caataaacgg
    15661 aatcccaatt tttccaaaag acatacactt tgatccttac tcaaacaagg attcaaacgt
    15721 tattcatttt cttgtaactg gcgaggagct aaagatcctc aagcaaaaga tcggatctga
    15781 tgagcacacc atgattgtgc aaataatgcc agacacaagc gttgcaatca aatcaaccga
    15841 tgtcacattc tcaaacggct acaaggcagc agtctcatac gacacaagat acggtgcaag
    15901 caaagatgtt tcatttacaa ttgcgttttt tgactctgca ggaaatctag caaaagacat
    15961 cagatatgca tacgctgtaa aagactcgac aggtgacgag ttcatcgtca acacaggagg
    16021 aaacacaaat cgacttggaa tctcgcttgc aagcggcgtt gattcaaggc taatcacaat
    16081 tccatcagag ggaagctatg cactacaact ggttctcata ggtaaaggct tgattgattt
    16141 tgatccgttt gttccagcaa gcatgcagtt tgatatctca aaaattgcat ctgctgcgcc
    16201 ttcaacgccg cctacgcagg ctgccattcc aaactggatt aaaaatagcg ccaagtggtg
    16261 ggcagacggc acaataggtg attctgactt tgtgtatgga attcagtttt tgataaagga
    16321 gggaatagta aagatcccac caactacggc aggtgaggcg tcctcaaacc agataccagt
    16381 ctgggtcaaa aaaaacgcca agtggtgggc agacggcaca ataggtgatt ctgactttat
    16441 ttctggcatt caatatctga taaaacaagg aataattcga gtctaaacta ttatccgaat
    16501 cccaacctca aataattggc cgtaatcaag atttcggaaa attgtttttc aaaaaacaca
    16561 aacaaccggt tttaaaatta tctggcacat ccatgctatg ctttcgcaga gagaatcaaa
    16621 acacagcccc aatgagacat gggtaacctg tagaaaatgc agtcaaaaga tgaaactttc
    16681 gtctttgggt gggacattat acagggaaaa ctggtaccat ctgtcatgtt ggaaatcact
    16741 cgtaggtaaa ttctaaccta aaaattttag acattatctc caccaagatt ttttattatt
    16801 acgttaaatt ttgataatta tgatgagcgt ataaaaaagg aaaagtacag cctaatcaaa
    16861 catgcgcagt atcgcgcaat tctgatgatt tgatagtatg atttcttagc gttcctttaa
    16921 ttgttgaaat gcgttagaat atttggtgag caggacgatc tcttgtcggg cttgcctgat
    16981 atcatgtgaa cctgcttgcc tgacaaattt gttttaaatg tttagaatat gaaaataaaa
    17041 tcttcattat tcttaatagc attgtttttg caacttaggt aacgtaaaac aagcactctt
    17101 gttttatgcg acctgtatta aggtcactgg caccctcgtg aaacgttgat cagtaagcat
    17161 tggtctacca gcccgaagtt gctattggca tcatgacttt gtgccagtga cattttttgc
    17221 ataattatcg tctaatctaa ttgcaaagaa aaaaaaagta ggagatagtt ggggatagga
    17281 tttttctatc tcctacctgt ttcagaaaat atcttctgca actttgatgc gagctctttt
    17341 tttgcctcag attgagtaag catagaactc catactttgc ctattgtctc aaagacggca
    17401 tctgctgctt tatccatcgt cggaccgt
//
LOCUS       DASWKH010000031         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_814090, whole genome shotgun sequence.
ACCESSION   DASWKH010000031 DASWKH010000000
VERSION     DASWKH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814090"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VGA92_02455"
     CDS             <1..846
                     /locus_tag="VGA92_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9845307.1"
                     /translation="HFDKKYKTLKILGSTEETTAGVTRIKAIERKGKLRYPVIVVNEA
                     YTKHMFDNRYGTGQSTIDGLLRAMNLLLASKRVVVSGYGWVGKGVAARSHGMGSKVIV
                     TETDPVKALEAHMDGFEVMSMAEAAKLGDIFITCTGMRDIIRKEHILKMKDGAVMGNV
                     GHFDVEIDSDFLLNKSKSVREVRPNLDECTLQNGKKVYLIGKGRLANLVAAEGHPPEV
                     MAQSFSNQILSILYILKNHKKIGKHVITVPAEIDTQIAIDALDAFGVKIDKLSTKQEK
                     YRSSW"
     gene            881..2527
                     /gene="metG"
                     /locus_tag="VGA92_02460"
     CDS             881..2527
                     /gene="metG"
                     /locus_tag="VGA92_02460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9845308.1"
                     /translation="MNLKAIITSALPYANGEIHLGHVASTYLPADVTTRFLKLNGVEA
                     YYVCASDDFGTPILIQAEREKKTPQEYVAHWNRRDYEDFTAFDIDFDFFYKTSSQENI
                     EFVQYVFKKLKENGYIYESEIIQFYCQNDKKFLPDRYVVGTCPYCKAQDQYSDLCEKC
                     GRVPEEIENPKCAICGAVPVKQKTMHYFFKLKNFGDKLTKWLEENEKLQKDVKKYVQN
                     WIASGLVDWDITRDISWGVPIPGAESKVFYGWFDNHLAYISSALKFLNDKGINGKEFW
                     NSADIYHFIGKDIVYHHFLFLPAMRLGIDEEYKLPDYIPTRGHLTLQSKKISKSRNWY
                     IGLKEFLGYYPADYLRFYLVSINPYSQDDLNFDWDDFAVRINSELIGNLGNLINRALG
                     FTKKSFDGVIPTPSEFDEKDKEAEQKIKGLSSELSTLMAQNHLDRALKKIMEFSMFFN
                     QYFQHKEPWKKGPGTNACVYLSVNAVGSIAIALYPFLPTSSEKIWGQLGLSGKIMDKS
                     WNSISELMLKPSHKLGDVSPLFAKVEDADIKKRKEKFATN"
     gene            2514..3266
                     /locus_tag="VGA92_02465"
     CDS             2514..3266
                     /locus_tag="VGA92_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HEX9845309.1"
                     /translation="MPQTKRIPRISTRGFFDLKTGKTLKKKSYDLYPKKFFENIGGFP
                     EFTIMVHGLRNNKEGALAKFKIAQQRLRHLGYKYPVIGFSYDSNVKNAHIKSYETRAM
                     EIGKVIARKNAINLAKFILYCKKQNPNARIRLMGHSLGSEVIIYALDKLKRKKNIIEA
                     VYFFGGSIPANSIHPQKFGKVLQNTVARKILNYYSPHDEVLKHAYENNTTEKPIGYLG
                     KEGKAVPKYVQKQVRPRNHRFASYAAVLRSYP"
     gene            3305..4222
                     /locus_tag="VGA92_02470"
     CDS             3305..4222
                     /locus_tag="VGA92_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX9845310.1"
                     /translation="MVKSVNFVVLGDQTIASQFGKKGTATDMTMYDKKEAGVIRTYTA
                     PNGFPEKIQPLLQTINLAEYVIIHVNKLDKFTGEQIIALDSLKKKDGILSHSFEVDEN
                     TLNSMIKNTVLENYTKVESDKIKEQSDKLDQVSKDGNARVVIDHCFDVKGVGTVILGK
                     VEQGKIKQYDNLKLLPAGIDVLVKSIQMHDDPVEEAVSPGRVGLAVKGVTPDQVSRGD
                     MLCAAAEQTSTELELDFTKSPFYKGEIAPNQMCLVNVGLQIKPAKFSSTTPLKLTLEK
                     PVVCTKGDICVILKPESQTIRILGSGTIK"
     gene            4266..4388
                     /locus_tag="VGA92_02475"
     CDS             4266..4388
                     /locus_tag="VGA92_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845311.1"
                     /translation="MKGLRACPICPACGSKKFEKLEDKDHVKVRCLSCNKIISI"
     gene            4428..>4750
                     /locus_tag="VGA92_02480"
     CDS             4428..>4750
                     /locus_tag="VGA92_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9845312.1"
                     /translation="MAKTWKDSDISLDPIKNQTIAVIGYGIQGDAQANNLKDSGLKVI
                     VGLKEGSPTWNKAKSDGHTVMSVAEATKKGDIVHILIPDMIQARVYREEIGPNLSKGK
                     ALSFS"
BASE COUNT         1621 a          921 c         1005 g         1203 t
ORIGIN      
        1 cactttgata agaaatacaa gacactcaaa attcttggct caacagaaga gactactgca
       61 ggagttacaa gaatcaaggc aattgaaagg aaaggaaagc tccgatatcc tgtaattgtt
      121 gtaaatgaag cttacacaaa acacatgttt gataataggt atgggactgg acagagcaca
      181 attgatggat tgctgcgcgc aatgaatctc ttgcttgcat caaaacgcgt agttgtatcg
      241 gggtatggat gggttggaaa aggtgttgct gcgcgctctc atggaatggg ctcaaaggta
      301 atcgttactg aaacagaccc tgtcaaagca ctagaggccc acatggatgg ctttgaggta
      361 atgtcaatgg cagaggctgc aaagcttgga gatattttca ttacctgcac tggcatgcgt
      421 gatataatca gaaaagagca cattttgaaa atgaaagatg gtgcagtcat gggaaatgtt
      481 ggacactttg atgtggaaat tgacagtgac tttttgttaa acaaatcaaa atctgtccgc
      541 gaggtacgac caaaccttga tgagtgcaca ttacaaaacg gtaaaaaagt ctatcttatt
      601 ggcaaaggaa ggcttgcaaa tcttgttgca gcagaaggcc atccgccaga ggtaatggct
      661 cagtccttct caaatcaaat tctttctatt ctttacatcc tcaaaaatca caaaaaaatt
      721 ggcaagcacg taatcacagt acctgctgaa attgacaccc aaatagcaat tgatgcactc
      781 gatgcgtttg gcgtcaaaat agacaagctt tcaacaaaac aagaaaaata ccgaagcagt
      841 tggtaaacct tcttaagctg cacgactcca agttcattta ttgaatttaa aagcaatcat
      901 tacaagtgca ctgccgtatg caaatggcga aatacatctg gggcatgtcg catcaacata
      961 tcttccagca gatgtcacga cgagatttct aaaactaaat ggtgttgagg cttactatgt
     1021 ttgcgcatcg gatgattttg gaacaccaat actaatccaa gcagaacgag aaaaaaaaac
     1081 acctcaggaa tatgttgcgc attggaaccg gcgtgactat gaggatttta ctgcctttga
     1141 cattgacttt gacttttttt acaagacaag ctcgcaagaa aacatagagt ttgtacaata
     1201 cgtgttcaaa aaactaaagg aaaatgggta catttacgag tccgaaataa tccaatttta
     1261 ctgccaaaac gacaaaaaat tccttcccga caggtacgtt gtaggtacgt gtccatactg
     1321 caaggcgcaa gaccagtatt ctgatctctg tgaaaaatgc ggccgtgttc cagaagagat
     1381 cgagaatcca aaatgcgcaa tttgcggcgc agtaccagta aagcaaaaga cgatgcatta
     1441 tttttttaag ctgaaaaact ttggcgacaa gctgaccaag tggcttgagg aaaatgaaaa
     1501 actgcaaaaa gatgtcaaaa aatacgtcca aaactggata gcatccggac tagtagactg
     1561 ggatataacg cgagatattt cgtggggtgt tccaatacct ggggctgaat caaaggtgtt
     1621 ctatgggtgg tttgataacc acttggcata catttcaagt gctctaaaat tcctaaacga
     1681 caagggaata aacggaaaag agttttggaa ttcggctgac atttaccact ttattggcaa
     1741 agacatagta taccatcatt ttttgttttt gcctgcaatg cgcctgggaa ttgatgagga
     1801 atacaaactg ccggactata ttccgactcg tggccatctt acactgcagt caaaaaagat
     1861 ctcaaagagt cgcaactggt acattggcct aaaggagttt cttgggtact atccagcgga
     1921 ctatctacgg ttttatctgg tatcaataaa cccatactca caggacgatt tgaattttga
     1981 ctgggatgat tttgctgtac gaatcaattc agagctaatt ggaaatcttg gtaatctgat
     2041 taatcgtgcc ttggggttta caaagaaaag ttttgacggc gtgattccaa cacctagtga
     2101 attcgatgag aaggacaaag aagctgaaca aaaaatcaag ggcctctcct cagaactctc
     2161 aacactaatg gcgcaaaacc atctggacag agctctaaag aagataatgg agttttccat
     2221 gttctttaac cagtattttc agcacaaaga gccgtggaaa aagggccctg gaactaatgc
     2281 ctgcgtatat ctttccgtaa atgccgtggg ctcaattgcg attgcactgt atccattttt
     2341 gccaacatct tctgaaaaaa tctggggcca gcttgggctg tcaggaaaaa ttatggacaa
     2401 gtcttggaat tccatttctg agttgatgct caagccgtcg cacaagcttg gtgatgtatc
     2461 gccattattt gccaaagtag aagatgctga catcaaaaag cgtaaagaaa aatttgccac
     2521 aaactaagag aattccaaga atttctacgc gaggattttt tgaccttaaa acaggaaaga
     2581 cactaaagaa aaaatcatac gatctttacc caaagaagtt ttttgaaaat attgggggat
     2641 ttccggaatt cacaataatg gttcatgggc tgcgaaataa caaggagggt gcactagcaa
     2701 aatttaagat tgcgcaacaa agattacgac accttggata caagtatcct gtcattggtt
     2761 ttagttatga ctccaatgta aaaaatgccc acattaaatc atatgaaaca agagcaatgg
     2821 aaattggtaa ggttattgca agaaaaaacg caattaatct tgcaaaattt attttatatt
     2881 gtaaaaaaca aaatccgaat gcacggattc ggctgatggg tcactccctt ggctctgagg
     2941 tgattatcta tgcacttgat aaactcaaac gcaaaaagaa cataattgaa gcagtctatt
     3001 tttttggagg ctcaattcct gcaaactcga ttcatccaca aaaatttggc aaggttctac
     3061 aaaacaccgt tgcacgaaaa attctcaatt actacagtcc gcatgacgag gtcctaaaac
     3121 acgcatacga aaacaacacg actgaaaaac caattgggta tttgggaaaa gaaggcaaag
     3181 ccgtgcctaa atatgtgcaa aaacaagtaa ggccaaggaa ccatcgcttt gccagctatg
     3241 ccgcagtctt aagatcatat ccgtaatagc tagcttgatt actggataat ttttggataa
     3301 aattatggta aaatcggtga attttgtcgt actgggcgac caaaccattg catcccaatt
     3361 cggaaaaaaa ggaactgcaa cagacatgac aatgtatgac aaaaaagaag ccggagtgat
     3421 tcgaacctat actgctccaa acgggtttcc tgaaaaaatt caaccgcttt tgcagacaat
     3481 caacttggca gaatatgtga taattcatgt aaacaagctt gacaagttta ctggcgagca
     3541 gataatagcg cttgacagcc tcaaaaagaa ggacggaatt ttatcacaca gcttcgaggt
     3601 ggatgaaaac acgctaaact ccatgataaa aaatactgtt ttggaaaatt atacaaaagt
     3661 tgaatctgat aaaataaaag aacaatcgga caagcttgat caagtatcaa aagacggaaa
     3721 cgcaagggtt gtaatcgacc attgttttga tgtcaaaggt gtcggaactg taatcttggg
     3781 aaaagtagaa caaggcaaaa taaaacagta tgataatctc aagcttttgc cggcaggaat
     3841 tgacgtgttg gtaaaatcca tccagatgca cgatgatcca gtagaagagg cagtatctcc
     3901 gggcagagtt ggccttgcag taaaaggcgt aactccagat caggtgagcc gaggtgatat
     3961 gctatgtgcc gcagcagagc aaacctctac agaacttgag ctggacttta ccaaaagccc
     4021 attttacaaa ggagaaattg cgccaaacca gatgtgtctt gttaatgtag gcctgcaaat
     4081 caagcctgcc aagttttcct caacaactcc actcaagctt acgctcgaaa agccagttgt
     4141 gtgtaccaaa ggcgatattt gtgtaatcct aaaacccgag tcacaaacaa ttaggatcct
     4201 tggaagcggc acaatcaagt aacaaattca acaataatta attagatgaa ttttgatctt
     4261 catacatgaa aggactaagg gcgtgtccca tctgccccgc ttgtggctct aagaaatttg
     4321 aaaaactaga agacaaagac cacgtcaagg ttcgctgcct ttcatgcaac aaaatcatat
     4381 caatttaatt tcgatggttt ttattcccag ttaatttagg caaaaacatg gcaaagacat
     4441 ggaaggactc tgatatcagc ttagatccaa taaaaaatca gacaatagca gttattggtt
     4501 atggaattca gggcgatgcc caggcaaaca acctcaaaga ttccggactc aaagtaattg
     4561 tcgggctcaa ggagggaagt cctacttgga acaaggccaa gtctgacggc cataccgtaa
     4621 tgtcagttgc agaagccaca aaaaaaggcg acatcgttca cattttgatt cctgatatga
     4681 ttcaggccag agtttaccgc gaagaaattg gcccaaacct atcgaaagga aaggcattat
     4741 cgttttctca
//
LOCUS       DASWKH010000032        12917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1181399, whole genome shotgun sequence.
ACCESSION   DASWKH010000032 DASWKH010000000
VERSION     DASWKH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 12917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12917
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181399"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VGA92_02485"
     CDS             complement(<1..942)
                     /locus_tag="VGA92_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9845313.1"
                     /translation="MEQHLEPVYRTSPERTAKMLAIMVGICIAGGVLFFAFWDYWISL
                     PPGNQMMDQEKQQGPAAATGKEIRQTMKFVESADFRTLAFNALPGEAGNNPDIQANVG
                     DKIIFDVTNDGKSFHSFGVTPSTEGFESILAGTAIGSPNSPLKPGEGGQATFVPTSEG
                     TFYYICTVPGHRELGMEGKIIVGPKQAPAEAAAPTGVSHDFNLDFIESADFKTYAFNA
                     LPGEAGNNPEFKVKSGDSVTFKVANKGKSFHSFGIVSDVDDPNSVVFSSAIKSANNPM
                     KPGESGEVTFTAGAPGTYYYICTVPGHSALGMKGSFIV"
     gene            complement(953..2479)
                     /locus_tag="VGA92_02490"
     CDS             complement(953..2479)
                     /locus_tag="VGA92_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9845314.1"
                     /translation="MVLELQKPRPIWQIMFSTHHTDIGLLYTISSITFFFLGGALALG
                     IRAELFLPGSQIIADSMTFNRMFTVHGTTLIFLFLLPFASGVGNYFVPIMVRYKDMAY
                     PKLNAIAFWMIPPAGALIWLGFADFTWYATPPYSSIRAPGPAADMWIFGLKILGVSSV
                     LGAINFIVTIMKCKHPDLPLSKVPLLAWSFLTSSLIVIVAIPTFAAALVMLLTDRLGV
                     SGFFDPSKGGDPIAYQHLFWFTFHPEVYVLVIPAIGMMYEIIPRFSRKPIFSQSSGIF
                     AFVMLSIVSFSSWAHHMYATGMSFTEKTVFMIGTLAAVPASAMHVFNFLATMWGGRIK
                     FATPMMWSVGGIALFFSAGAGGVANSAMPFDFITHDSYWVVGHFHLFVMGTIAFGSIG
                     FLYYMFPYITGRMYNEAMGKVHFIMAFIGTVMVFFTQHVLGLYGMPRRVFDYMPLPEY
                     IVMNQIASVGAMIIGVSMGLWIFNMIYSSAKGKEANMEDPWGLGGKYYFPYQAKTPHH
                     "
     gene            complement(2529..2951)
                     /locus_tag="VGA92_02495"
     CDS             complement(2529..2951)
                     /locus_tag="VGA92_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845315.1"
                     /translation="MSGHSNWPEWIYIGVVVALLVYVGAEAWNVERLVEHAPAEAEVI
                     KVTGQQWFWTFERADGTKEVGELHVKKGQPYKFEIAAKDVNHSFNIHDYVVLQDAVVG
                     RINTVWFVPDKAGEYTIQCREYCGLLHYNMRATLYVEE"
     gene            complement(2952..3215)
                     /locus_tag="VGA92_02500"
     CDS             complement(2952..3215)
                     /locus_tag="VGA92_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme transporter CcmC"
                     /protein_id="HEX9845316.1"
                     /translation="MARAIILMSVLAVLVVLPTLQLALGAELDGGEYIQRKVEIWNLF
                     FKMMTIAFTVGAVVSGTIIWLVWRFRESHPKAKPTKYEEKGEW"
     gene            complement(3340..3966)
                     /locus_tag="VGA92_02505"
     CDS             complement(3340..3966)
                     /locus_tag="VGA92_02505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9845317.1"
                     /translation="MGLLIFLRHGQAQNNTARVLAGRAPGVSLTKTGIEQANDIGKFL
                     KSFDVSHIYASPIERAKTTAEIVGSHLDLPTTIDERLFELEMGKFSGMEYDELFAKHG
                     NVFLKFYQGDPTIAENGVETFPQVKKRVLDLVDFACKQHTGKNVLFVTHMDPIKAMIS
                     NILNLSPHSLFELIIANASLTIVREEQGKLSLSAINAMNSQRYIQGPF"
     gene            4022..4693
                     /gene="npdG"
                     /locus_tag="VGA92_02510"
     CDS             4022..4693
                     /gene="npdG"
                     /locus_tag="VGA92_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX9845318.1"
                     /translation="MKIGIIGGTGGMGKGFALRWCPKHEILVGSRDAQRAATAASEYL
                     DFAKQIYGNISGTISGTDNVSVAKESDVLILSIPYENIDSVCSELLGKVKDGCTVISP
                     IVPMVKTETGFEFIPIKEGKPFSHQLVQKYMKNKAKLVSAFHVISEKKLVDPNLTLDY
                     DIFVCGDDKESIDVVNALISEIKGLRPIFLGSGALAYMAEIATPLLLNAMIKNKLKNP
                     GIKIL"
     gene            4738..5001
                     /locus_tag="VGA92_02515"
     CDS             4738..5001
                     /locus_tag="VGA92_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9845319.1"
                     /translation="MKEIVMLKIVSVLASGQISIPVDIRKMLGIKKGDRLLLTVASKR
                     ILIEKLSEITEKIEDDFSCLIKISERTAKKLWNNESDEIWNRL"
     gene            5050..5277
                     /locus_tag="VGA92_02520"
     CDS             5050..5277
                     /locus_tag="VGA92_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845320.1"
                     /translation="MESSRPKKGRNWYSAMGILFIVIGAIVMVRNVLIWSPDFVLDFL
                     LNSEITNEKISVGMFVFGGFLLFLGFRKKVV"
     gene            5270..5668
                     /locus_tag="VGA92_02525"
     CDS             5270..5668
                     /locus_tag="VGA92_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9845321.1"
                     /translation="MFKKEKFLKEQKILHLATLDAKGNPHLVPVWYNYVGKKFYVGTN
                     TRTAKAKNIQKNKNICFCVDAGIWSPIHGIMGSGRAKMILEKNQVKKIAGKILLRYFK
                     SLDEKTAKQLLDDTDCIIEIVPTKITSWHY"
     gene            complement(5652..6833)
                     /locus_tag="VGA92_02530"
     CDS             complement(5652..6833)
                     /locus_tag="VGA92_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9845322.1"
                     /translation="MKVSKKVSGVEYAIRDIVLAARQVEKQGKKITYLNIGDPVQYGF
                     QPPDNVKEAMINAIRNGQNYYASSEGLPELREAIAKKESAKGLSVGSDDILVTNGVSE
                     ALDMVMSSIVEEGDEVLLPGPYYPPYASYVRLHGGIPVEFAVDLKNSTPDIDDIKSKI
                     TPKTVAICLISPNNPTGVVFSEQSLKKLVDIANEHDLYIICDEIYDQIVFDEKFTGIG
                     KVAGDSPVILLNGFSKVHLMSGWRIGYIAFNNSQKLAPLRQNLPKLGRVRISTNLPVQ
                     YAALESLRGPQGYVSEFVSELKKRRDFVVKRLNSVSGLSCSNPRGAFYAFPKIENNRY
                     KSDEEFVMELLKKTGVLTVHGSGFGTKYGSGHFRIVFLPDLNTLSFALNEIEKFVSNA
                     S"
     gene            7078..7689
                     /locus_tag="VGA92_02535"
     CDS             7078..7689
                     /locus_tag="VGA92_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845323.1"
                     /translation="MSYYELWTILSNWRDEFTSEDFARTFASPNPRKVLHDMTGKGLL
                     EHRGYGRYRVRPVDEYVRSKNDVSSGYELLKKSDLPYALTDVDGVFVWTKGGYNAGRF
                     FGSYPIHMKVLKSDVARWKRFLTEKGRKVTLGGKTPRETLFGVYYILYPEVKVKSETE
                     GGLKVEPLKDTVEFCKKNIYTFEPALEMLDEEYNLGLKVRYSR"
     gene            7694..8395
                     /locus_tag="VGA92_02540"
     CDS             7694..8395
                     /locus_tag="VGA92_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845324.1"
                     /translation="MSQKEDLTAREAEVIRILKVLGAKSSDFVVIGGYAINALTSHRF
                     SVDCDIVTDSKQMKEISKILADEGYKALSRVRKQSIYGVKMKKYVKPVGTRKVSVEVL
                     PNEVSCRQTDGTWSFESIMQNSTKLRVVGLTDSAHALVPKKELLMAMKTHAGRDTDLR
                     DVVMLSERADWKAVKEFASRGSIDKVKKQIESAIATISKQEFASALKAEFGLRQDVSP
                     IIKRTLDGLEKLKSH"
     gene            complement(8777..9007)
                     /locus_tag="VGA92_02545"
     CDS             complement(8777..9007)
                     /locus_tag="VGA92_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9845325.1"
                     /translation="MLKFPKLSGKDVVKVLVKEFAFETTRQTGSHVVLRKFVAGKKIV
                     TIVPLHRELKTGTLLGVLGLAKIEKDDFLKHL"
     gene            complement(8991..9200)
                     /locus_tag="VGA92_02550"
     CDS             complement(8991..9200)
                     /locus_tag="VGA92_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011250462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845326.1"
                     /translation="MKLQTILWKEDDVYVIKEAITGVTTQGSTVEEALTNLKEAVSLY
                     VDEVPEAREIITKATTVGALSVEIP"
     gene            9398..9472
                     /locus_tag="VGA92_02555"
     tRNA            9398..9472
                     /locus_tag="VGA92_02555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9433..9435,aa:Arg,seq:tcg)
     gene            9599..10771
                     /locus_tag="VGA92_02560"
     CDS             9599..10771
                     /locus_tag="VGA92_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9845327.1"
                     /translation="MSFSLYSVSGKDFIGRKEIIKELVGELASKNRIGFSLSGVRRIG
                     KTSILKETKRVLEKKGVVVIYISAWSIVPPTVDELTKMLSRTVIAEFSKKLPKKFKFE
                     QLLVTGAKALATFLQNLKLTSSVAKDLEISISYIRKESDDVDGAIKKSFSLLEDVAKM
                     TNTKSVLIIDEFPSLVDLTYGTKNQKIGNEVVRLLRTLFEDFRYTKLVVSGSFRDTLE
                     NLITKERAPFYKQLLLRQIQPFDEREFEAFLQHYLPRLKFSNDDVKIKLYNITSGIPY
                     NLQLIGKEIQYLGLAKLDEQKLDKVIEDVLKKEGEQSFKEFIAGLSPSEIKVLKGLVR
                     NPGIAPVEISRREFIDENSVSACLNTLEKKSIIKKIERGKYDFTDNLFAAWLNQNL"
     gene            10938..11156
                     /locus_tag="VGA92_02565"
     CDS             10938..11156
                     /locus_tag="VGA92_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845328.1"
                     /translation="MKKRLETEKQAVSLAVSGIVKKYGVTPSDIKTLEIKYDDEAGDW
                     YVALGFGGKRTVVKMDSVHATISEINEM"
     gene            complement(11387..12019)
                     /locus_tag="VGA92_02570"
     CDS             complement(11387..12019)
                     /locus_tag="VGA92_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX9845329.1"
                     /translation="MSAVTVEQVRNSLTKCMDPEVPLNIVEMGLIYGIDITESNDVHI
                     KMTMTTQGCPLHQTLVQDVTRYTKKVPGVNNVQVDIVWDPPWTIDKMSDEARAKLKGF
                     TSKGTPAPIDYDTAMPQGIGSLVQQEDGSMVLANEHNQGFMVNEAIVDFWKSCDGKRK
                     ITELVDIFAQKTGLQRSQIEKEVIQLIGQLRDGGLIVIPTQEAPNVEFRK"
     gene            complement(12055..>12917)
                     /gene="mtnA"
                     /locus_tag="VGA92_02575"
     CDS             complement(12055..>12917)
                     /gene="mtnA"
                     /locus_tag="VGA92_02575"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9845330.1"
                     /translation="ALAALQSKATTKDQLLADLETARKILFETRPTAINLAWGLNKIM
                     SVAKEGNDVSEIKNSIIKTAQQMAEDDIKINMQMGKNGSILFDDNDTIMTHCNAGALA
                     TVAYGTALGVVRATREAGKSIKVIATETRPVQQGSRLTAFELKHDGIDVSLIPDTAVG
                     YTMANKLVNKVIVGADRVLRTGHVFNKIGTYQVAMMAKQHGIPFYVAAPLSTFDMTSN
                     PKDVVIEQRKASEVTQIGDKKTAPDGINVINPAFDMTPPELVSGIITEAGIAKPPFEE
                     SIKKLFAANK"
BASE COUNT         3695 a         2615 c         2723 g         3884 t
ORIGIN      
        1 tactatgaaa ctacctttca ttcccagtgc agagtgacct ggcactgtac aaatgtaata
       61 atatgttcct ggtgctcccg cagtaaatgt cacctcaccg ctttcacccg gcttcattgg
      121 attgtttgca gacttgattg cagagctaaa gaccacactg tttggatcat ccacatcaga
      181 tacaattcca aatgagtgga aagatttgcc cttgtttgca actttgaatg taacagagtc
      241 gccagacttt accttgaatt ctgggttgtt tcccgcctca cctggcaatg cattaaacgc
      301 atatgtctta aagtccgctg attctatgaa atccaaatta aaatcatggc tcacaccagt
      361 tggtgccgct gcttcagcgg gtgcctgttt tgggccaact atgatttttc cttccatacc
      421 gagttctctg tgtcctggga cagtacaaat gtagtaaaaa gtaccctcac ttgtcgggac
      481 aaatgtagcc tgcccgcctt cacctggctt tagtgggctg tttggacttc ctattgcagt
      541 gccagcaaga attgactcaa atccttcagt tgagggtgtc actccaaatg agtgaaatga
      601 cttgccgtca tttgtgacat caaaaattat tttatcgccc acgtttgcct gaatgtcggg
      661 attgtttcct gcctctcctg gaagtgcgtt aaatgcaagt gttctaaagt cggcggattc
      721 gacaaatttc attgtctgtc ggatctcttt tcctgttgca gctgctgggc cttgttgctt
      781 ttcctgatcc atcatctggt ttccaggcgg cagtgatatc caataatccc aaaatgcaaa
      841 aaagagtacg cctcctgcaa tgcaaatgcc gaccatgatt gcaagcatct ttgctgtcct
      901 ttcaggcgag gtcctgtaga ctggttctag gtgttgttcc attttttatc tcctagtggt
      961 gcggtgtctt ggcctgatat gggaagtaat acttgccgcc aagcccccac gggtcctcca
     1021 tgttggcctc tttgcctttt gcagagctgt agatcatatt gaaaatccat agtcccatgc
     1081 ttacgccaat tatcattgca ccaactgatg caatctggtt cattacaata tattccggta
     1141 atggcatata gtcgaaaacc cttcttggca ttccatagag tccaagtacg tgttgtgtaa
     1201 agaagaccat caccgtccca ataaacgcca tgataaagtg aaccttgccc attgcctcat
     1261 tgtacattct tcctgtgatg tatgggaaca tgtagtatag gaatccaatt gagccaaatg
     1321 caattgttcc catcacaaat agatggaagt gtcctaccac ccagtacgaa tcatgtgtga
     1381 taaagtcaaa cggcattgcg ctgtttgcaa ctccgcctgc tcctgctgag aagaataatg
     1441 ctataccgcc aactgaccac atcattggtg ttgcaaactt gattcgcccg ccccacatag
     1501 tggccaaaaa gttaaagacg tgcattgcag atgccggtac tgctgcaagc gttccaatca
     1561 taaatactgt tttttctgta aacgacattc ctgttgcgta catgtggtgc gcccaagatg
     1621 aaaatgagac aatgctcagc attacaaatg caaagattcc agaggactgg ctaaagatcg
     1681 gctttcttga gaaccttgga atgatttcgt acatcatgcc aattgccgga attacaagaa
     1741 catacacctc gggatggaat gtaaaccaaa acagatgctg gtacgcaatt gggtctcctc
     1801 cttttgatgg atcaaaaaat ccgcttactc ccagtctgtc tgttagtaac attacaagcg
     1861 ctgctgcaaa tgtaggtatg gcaacaatta caattagcga agatgtcaaa aacgaccaag
     1921 caagaagcgg caccttgctt agaggcaaat caggatgttt gcatttcata atggtaacta
     1981 taaagttaat tgcgccaagt actgatgata cgcccaagat ttttaggcca aaaatccaca
     2041 tgtctgctgc aggacccggt gcacgaattg aagaatatgg cggggttgca taccacgtaa
     2101 agtcagcaaa gccaagccag attagtgcac ctgctggtgg aatcatccaa aacgcgattg
     2161 cgtttagttt tggatatgcc atgtctttgt agcgaaccat tattgggaca aaatagttgc
     2221 caaccccaga tgcaaatggc aaaaggaaca aaaagataag cgttgttcca tggactgtaa
     2281 acattctgtt aaacgtcatt gaatcggcaa ttatttggga gccgggaagg aatagttctg
     2341 ctctaattcc cagtgccagt gcaccaccca aaaagaagaa tgtaattgac gaaattgtgt
     2401 atagcaatcc gatatcggtg tgatgagttg aaaacattat ctgccagatt ggacgcggct
     2461 tttgtagctc tagtaccatt tagttgtcca cctctacttc ttttattaag ttatcaatgc
     2521 ttttcatttc attcctccac atacaaagta gccctcatgt tatagtgaag caaaccgcag
     2581 tactctctgc actggattgt gtactctcct gccttgtctg gcacaaacca aaccgtgttg
     2641 attcttccaa ctacggcatc ttgcagtacc acataatcat gaatgttaaa tgaatggttg
     2701 acgtctttgg cagctatttc aaatttgtac ggctgtcctt tctttacgtg tagttctcct
     2761 acttcttttg ttccgtcggc acgctcaaag gtccaaaacc attgttggcc tgttactttg
     2821 atgacttcgg cttcggctgg ggcgtgttca actagtcttt cgacattcca tgcctctgct
     2881 ccaacataga ccaaaagcgc aactactacg ccaatgtaaa tccattcagg ccagttagag
     2941 tgtccgctca tttaccattc acccttttct tcgtattttg taggcttggc ctttggatga
     3001 gattctcgga atctccacac aagccagatt atcgtaccag agactactgc acctactgtg
     3061 aacgctatag tcatcatctt aaagaacagg ttccagatct ctactttgcg ttgtatgtac
     3121 tcgcctccgt ctaattcagc cccaagtgca agctgcaaag taggtaaaac aactagaaca
     3181 gctaaaactg acattaggat aattgccctg gccataacca attactgctc gtggccttcg
     3241 gattctattt aagggttttg agctttgtca ggtcggtaaa tactggtaaa ttgatcggtt
     3301 tgattacagc tactgaattt tgtgatattt tgtaaattgc taaaatggtc cttggatata
     3361 tctttgtgaa ttcattgcat tgattgcaga aagtgaaagt tttccctgtt cttctcttac
     3421 tattgtcagc gatgcatttg cgattattag ctcaaaaagt gagtgcgggc ttaggtttag
     3481 tatgtttgag atcattgctt tgattggatc catgtgggtc acaaacaaga cgtttttgcc
     3541 agtgtgttgt ttgcaagcaa aatccacaag atccaagact cgttttttca cttggggaaa
     3601 tgtttccaca ccgttttctg caattgtggg atcgccttgg taaaatttca aaaaaacgtt
     3661 tccgtgtttt gcaaataatt catcatattc cattcctgaa aacttgccca tttccagctc
     3721 gaacaatcgt tcatctattg tggttggaag gtctaaatgg cttccaacga tttcggcagt
     3781 agtttttgca cgctcaattg ggcttgcata gatgtgcgaa acatcaaagg attttagaaa
     3841 ttttccaata tcgtttgcct gctcgattcc agtttttgta agcgatacgc ccggggccct
     3901 tcctgccaaa actcttgctg tattgttttg agcctgaccg tggcgcaaaa atatgagtaa
     3961 tcccattgga ctttttgaat ttcaaataaa agataataat aacattgcct gccgtttagc
     4021 gttgaagata ggaataattg gcggaactgg tggcatgggc aaaggttttg cactacgctg
     4081 gtgcccaaaa catgaaattc ttgttggctc aagggatgcc cagcgagctg ctactgccgc
     4141 atcagaatat cttgattttg caaaacagat ctatggaaac attagtggaa ccatatctgg
     4201 tactgataat gtttctgtgg caaaagaaag cgatgttttg attttatcaa ttccgtatga
     4261 gaatattgat tctgtgtgct ctgaactgct tggaaaagta aaggatggct gcactgtgat
     4321 atcgccaatt gttccaatgg taaaaacaga aactggattt gagttcatac caataaagga
     4381 gggcaagcct ttctcgcacc agctagtcca aaaatacatg aaaaacaaag ctaaactggt
     4441 ttctgcattt catgtcatat cagaaaaaaa gcttgtcgat ccaaacctga cactagacta
     4501 tgacattttt gtctgtggtg atgacaaaga gtccattgat gtggttaacg cattgatctc
     4561 cgaaattaaa ggcctaagac caatcttttt gggctcaggt gcacttgcat atatggcaga
     4621 gattgcaacg ccgcttctgt tgaacgcgat gataaaaaac aagctcaaaa atccgggaat
     4681 aaagattctg tgacaaattc ttgttaatat tataagtgag aaatctctta cttgggtatg
     4741 aaagaaattg tcatgcttaa gatcgtaagt gttttggcaa gtgggcagat ttcaatacct
     4801 gtagatataa gaaagatgct tggaatcaaa aaaggagaca gattattact cactgtagca
     4861 agtaaaagaa ttctcattga aaaattaagt gaaataacag aaaaaattga agatgatttt
     4921 agctgcttga taaagatttc agagagaact gcaaaaaagt tgtggaataa tgaaagcgac
     4981 gaaatctgga atcgacttta agaagataaa aacaagttaa aaagcccaaa tatcagaata
     5041 gattagttta tggaatcatc ccgtccaaaa aaaggaagaa actggtattc tgcaatggga
     5101 atattgttta ttgtgatagg agcaattgtc atggtgcgaa atgtcctaat ttggagcccg
     5161 gactttgtct tggattttct tttaaattcc gagattacga atgaaaagat ttcagttgga
     5221 atgtttgtat ttggaggatt cctgttgttt ttagggttta ggaagaaagt tgtttaaaaa
     5281 agaaaagttc ctaaaagagc aaaagattct gcatcttgcc acacttgacg ctaagggcaa
     5341 tccgcaccta gttcctgtct ggtataatta tgttggaaaa aaattctatg ttgggacaaa
     5401 tacaaggact gccaaggcaa aaaatattca aaaaaacaaa aacatttgtt tttgtgtaga
     5461 tgccggaatt tggtcgccaa ttcatggcat tatgggctct ggcagggcga aaatgatact
     5521 agaaaaaaac caagtcaaaa agattgcagg taaaatttta cttcgttatt tcaagtcact
     5581 tgatgagaaa acagcaaaac agttacttga tgacacagat tgtataatag agattgtgcc
     5641 aaccaaaatt actagctggc attactgaca aatttttcaa tttcgtttag ggcaaagctt
     5701 agcgtattca aatcaggcaa aaacacaatt ctaaaatgcc cactaccata ttttgttcca
     5761 aacccagagc catgcactgt tagaactcct gtttttttga gtagttccat tacaaattct
     5821 tcatctgatt tgtacctgtt gttttcgatt tttggaaatg cgtaaaatgc accccttgga
     5881 tttgagcagg aaagtcccga gacagaatta agccttttta ccacaaagtc ccgccttttt
     5941 tttagctccg atacaaactc agatacgtat ccttgtggac ctcgaagcga ttccagtgca
     6001 gcatattgga ctggcaggtt tgttgatatt cttacgcgcc caagctttgg caagttttga
     6061 cgtaatggag ctaatttttg cgagttgtta aacgcgatat accctattct ccagccggac
     6121 atcaagtgaa ccttggaaaa tccgtttaac aggattactg gcgaatctcc tgccaccttg
     6181 ccaatacctg tgaatttttc atcaaataca atttggtcgt atatctcgtc acaaattatg
     6241 tacagatcat gctcgtttgc aatgtctact agctttttta gtgattgctc ggaaaatacc
     6301 acacctgttg gattgtttgg actaattagg cagattgcaa ctgtctttgg tgttattttt
     6361 gatttgatat catcaatgtc aggtgtagag tttttcaaat caactgcaaa ctctactgga
     6421 atgccgccgt gcagcctcac atatgatgcg tatggcggat agtaggggcc aggaagcaag
     6481 acctcgtctc cttcttctac aattgatgac attaccatgt ctagtgcttc tgagacaccg
     6541 tttgtgacaa gtatgtcgtc tgagccaact gagagtcctt ttgcgctttc tttttttgct
     6601 attgcctctc gcagctcagg cagtccttct gatgatgcgt aatagttctg gccgtttctg
     6661 attgcattta tcattgcttc tttgacattg tctggtggtt ggaagccgta ctggactggg
     6721 tcacctatgt taaggtaggt tatctttttt ccttgttttt ctacctgacg tgcagcaagt
     6781 acgatatctc taattgcata ttccactcct gaaacttttt ttgaaacctt caagtaacta
     6841 gacagatatt tagcctcgtt ttagtcttac ctctttttga cttatatcac agtctggata
     6901 gtgcaaacgg cttctttaat ttagaaataa ttatgtgcat ccatcattag cctttcaatt
     6961 acaacttaga ttacggagtt tttcttaaat tcaaagcatc ttcgatcgtt tcctaaatct
     7021 accaatacag actagcacgc cgccatcttt ataagatgtt actgtcatag taacattatg
     7081 tcgtactacg agctgtggac tatactgagc aactggaggg atgagttcac tagtgaggac
     7141 tttgctagga cctttgctag tcccaatccc agaaaagtcc tgcacgacat gacagggaag
     7201 ggtcttctcg agcaccgcgg ctacgggagg tacagggtca ggccggtcga cgagtacgtg
     7261 aggtcgaaga acgacgtgag ctccggatac gagctgctca agaagtctga tctgccgtat
     7321 gccctgaccg atgtggatgg ggtctttgtc tggacgaagg gcgggtataa tgctggtagg
     7381 ttcttcggct catatccgat ccacatgaaa gtcctgaagt cggacgtggc aagatggaag
     7441 aggttcctca cggagaaggg gagaaaggtc acgctcggcg gaaaaactcc gagagagacg
     7501 ctgtttggag tctattacat cctgtatccg gaagtcaagg tgaagtcaga gaccgagggg
     7561 gggctgaagg tggagccgct caaggatacg gtggaatttt gcaagaagaa catatacact
     7621 ttcgagcctg ccttagagat gcttgacgag gaatacaatc ttggcctcaa agtgagatac
     7681 agtagataat acaatgtcgc aaaaagaaga tctcactgca agggaagccg aagtaatcag
     7741 gatcctaaag gtgctcggcg cgaagagcag tgatttcgtc gtgatcggcg gttatgccat
     7801 caacgcgcta acgtcacaca ggttttccgt tgactgcgac atcgtgacag actcaaagca
     7861 gatgaaggag attagcaaga ttctggcgga tgaaggatac aaggcgctct cgagagtaag
     7921 aaagcagtcc atttacggag taaagatgaa aaagtacgtc aagccggtcg gcacaaggaa
     7981 ggtatctgta gaggtgctcc cgaacgaagt gagctgtagg cagactgacg ggacgtggag
     8041 ctttgagagt atcatgcaga attcgactaa actacgggtg gtaggtctga cagattcggc
     8101 acatgccctt gtgccaaaga aggagctact catggccatg aagacgcacg caggcaggga
     8161 tacggacctc agagatgtgg ttatgctgag tgagcgggca gactggaaag cggtaaaaga
     8221 attcgcgtcg cgcggcagca tcgacaaagt aaagaagcaa attgagtctg cgatcgcaac
     8281 gataagcaag caggagtttg cctcggcgtt gaaggcagag ttcggcctgc gacaggacgt
     8341 ctcaccgata atcaaaagaa cattggacgg cctcgaaaaa ttgaaaagtc attaaggtca
     8401 taaatgactt taatctttta gatgaaatct attctgttgg catacagata cacctagctg
     8461 acgaatatgc aatcctcgat attgttctag ttgtacgagg ggtagaaaaa acggaaaaac
     8521 atctaacttt tttgaaacct tcaagtaact agacagatat ttagcctcgt taaagtctta
     8581 cctctctgga ctcgtagcac aatctggata gtgctggtgg ttttaattcg cttaggaata
     8641 attggtgcat ccatggtcaa tctttcagtt acttgtccgg tttacattga tgaaatttga
     8701 atggaattaa ttagctcaaa gcatgcataa aatccttgtt aaaacattaa tgcaaaatca
     8761 ggattttaag aatggattat agatgcttta aaaaatcatc tttttcaatc tttgccaagc
     8821 caagaacgcc taatagcgta cccgtcttta attccctatg aagcggaact attgtaacaa
     8881 tctttttgcc tgcaacaaat tttcttaata caacatgact tccagtctgt cttgttgttt
     8941 caaaagcaaa ttcttttaca agaactttaa cgacatcttt tccagaaagt ttagggaatt
     9001 tcaacactta gtgcaccaac tgtagttgct tttgttataa tctcccttgc ctcaggaact
     9061 tcgtcaacat acagacttac agcttctttg agatttgtga gtgcttcttc aactgtgctg
     9121 ccctgtgttg ttacaccagt tattgcttct tttattacat atacgtcatc ttctttccac
     9181 agaattgttt gtaatttcac aagttattga tgtcaataga tgattttaat cttttagatg
     9241 agatctgttt tatcggcata gaaaacccag ttaggaaata ctagctggcg ttgaatatgc
     9301 aattcgtgac attatcctag ttgcgttagt agagaaacac ctacttgaaa tcttcaacta
     9361 gacagatatt tagcctcgtt ttagtcttac cccttttgga ctcgtagcac agtctggata
     9421 gtgcgggcgg cttcggaccg ccaggtcgag ggatcgaagc cctccgagcc ctctttttgt
     9481 agtgttacga tttggtttgc agctgatttt gtaatttttg atcgcagtat caaagatcaa
     9541 ctgccaagtt gtactaaaac ttataaagtt tataacttat aaaatctata aggaattgat
     9601 gtccttttca ctttattccg tctctggcaa ggatttcata ggtaggaagg aaataataaa
     9661 ggaactagtt ggggagcttg catccaaaaa caggatcggt ttttcactat cgggagtaag
     9721 aagaattggc aagactagca tcctaaaaga aacaaagcgc gttctagaaa aaaaaggcgt
     9781 tgtggtaatc tacatttcag cctggagcat agttccgcct acggtggatg aacttacaaa
     9841 aatgctgagt cgcaccgtaa ttgcagagtt ttccaagaaa ctgccaaaaa aattcaaatt
     9901 cgaacagctg cttgttacgg gtgcaaaagc acttgcaaca tttctccaaa acctaaaact
     9961 gacctcgagt gtggccaagg atctggagat ctccatttcg tatatcagaa aagaatctga
    10021 cgatgtggac ggtgcaatca aaaagtcatt ttcgttattg gaggatgtgg caaagatgac
    10081 caataccaaa agcgtgctga ttatagatga attcccatca ctagtagatc tgacgtatgg
    10141 cacaaaaaat cagaaaatag gcaacgaggt agttcgactt ttgagaactc tgtttgaaga
    10201 ctttaggtac accaaactgg tggtatcagg ctcatttcgg gatacgctgg aaaacctgat
    10261 aacaaaagaa agagcgccat tttacaaaca actgcttttg cgtcagatcc agccatttga
    10321 tgagcgtgag tttgaggcgt ttttacaaca ctacctgcca agactaaagt tctcaaatga
    10381 cgacgtaaaa ataaaactgt acaatatcac tagcgggatt ccctacaatc tgcaattgat
    10441 tggaaaggaa atacagtatc ttgggcttgc aaaactcgat gaacaaaagc tggacaaagt
    10501 cattgaggac gtcctaaaaa aggaaggtga acaatccttt aaggaattca tcgcaggtct
    10561 ttcaccctct gagataaagg tcctaaaagg gcttgtcagg aatcctggaa tcgcaccagt
    10621 tgaaatctcg aggagggaat ttatcgatga aaacagcgtt agtgcctgtc ttaacacact
    10681 tgaaaaaaaa tcgattatca aaaaaattga gaggggtaaa tacgatttta cggacaacct
    10741 atttgccgcc tggctgaatc aaaatttgta gtttttcaaa ttgacttgga aacacagcct
    10801 aaacgcacct gcaaaatcgc aaatctgata gttgagaatc tgcatgtaca agtgaaatta
    10861 aaggggcagc taaattgtca tcaccttaac tatattagaa gaatcatcgt acatcagcta
    10921 tgcccggaat aggattaatg aaaaaaagac tggaaactga aaaacaggca gtatctttgg
    10981 cagtttctgg aattgtgaaa aaatatggcg taacaccttc agatatcaag acacttgaaa
    11041 taaaatatga tgatgaggca ggcgactggt atgttgcgct aggatttggg ggaaaacgta
    11101 cagtagtcaa aatggattct gttcatgcca caatatcaga aataaatgag atgtaatgtt
    11161 ctttaagggt ttgaaattat acgcccatta atttctctcg gtattgtaac aatagacatt
    11221 ttttacacac acaatcatca aacctaatgt ctttttcatt cagttttgat tcctcaaaac
    11281 accaacacaa tatggtctcc tcacactcaa aagaagcccc acatcttttg cagtttttaa
    11341 tcaattgaga acatttcgtc atacgtacta aaatttttta tgatatctat tttctaaatt
    11401 caacgtttgg cgcttcctga gttggtatta cgattaggcc gccgtctctt agctgcccaa
    11461 taagctgaat cacttctttt tctatttggg agcgctgcag tcctgttttt tgggcaaaaa
    11521 tatctactaa ttcggttatc tttctttttc cgtcgcatga cttccaaaag tcaactatgg
    11581 cctcatttac catgaatcct tggttgtgtt catttgctaa aaccatggaa ccatcttctt
    11641 gctgtactaa tgagccgatt ccttgtggca ttgcagtatc ataatctatg ggtgctggag
    11701 ttcccttgga agtaaagcct tttagttttg ctcttgcctc atctgacatt ttatcaattg
    11761 tccatggagg atcccagaca atgtctactt gtacattatt gacgcctggt acttttttgg
    11821 tgtaccttgt tacatcttgg actagggttt gatgcagtgg acatccttgt gtagtcattg
    11881 tcattttgat gtgcacatca tttgattctg taatatcaat tccataaatt agtcccattt
    11941 caacaatgtt taatggtact tctggatcca tgcattttgt aagggagttt cgtacttgtt
    12001 caacggtaac tgcagacatg tttttaatct cttttcaaaa ataataaaaa ctttctattt
    12061 attagctgca aataactttt tgatagattc ttcaaatgga ggttttgcaa ttccagcctc
    12121 ggtaattatg cctgagacga gctcaggtgg agtcatatca aatgccggat ttatgacatt
    12181 gataccgtct ggagcagttt ttttgtcacc gatttgggta acctcgcttg cctttcgttg
    12241 ttcaattacg acatcctttg gattactcgt catatcaaat gtagaaagtg gggctgccac
    12301 atagaatggg attccatgct gttttgccat cattgccact tggtatgtcc ctattttgtt
    12361 aaagacgtgt cctgtacgga gcactctgtc ggcaccgaca attactttgt ttacaagttt
    12421 atttgccatt gtatatccta ccgcagtatc tggaatcaga ctaacatcaa tcccatcgtg
    12481 ttttagctca aacgctgtta gacgagagcc ttgttgtact ggtcttgttt cggttgcaat
    12541 tactttgatg ctttttccag cttctcttgt tgccctgact acgccaagtg ctgtgccata
    12601 tgctacggtt gctagtgcgc ctgcattaca atgagtcatt atggtatcgt tgtcatcaaa
    12661 tagaatagag ccgtttttgc ccatttgcat gttaattttg atgtcatctt ctgccatttg
    12721 ttgtgccgtt tttatgatgg aatttttaat ttcagaaaca tcgttacctt cttttgccac
    12781 actcataatt ttgttcagac cccacgcaag atttattgca gttggtcttg tctcaaatag
    12841 aattttcctt gctgtctcga gatcagccaa taattgatct tttgttgttg ctttgctctg
    12901 caaagcggca agtgcaa
//
LOCUS       DASWKH010000033         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3047467, whole genome shotgun sequence.
ACCESSION   DASWKH010000033 DASWKH010000000
VERSION     DASWKH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047467"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VGA92_02580"
     CDS             <1..918
                     /locus_tag="VGA92_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX9845331.1"
                     /translation="NTLALSSTHNLPAKVIAGFVDEAVNLLLDLNTQKEVSLALVPIG
                     FTKEIPTEKFLVQKLNLATESLSSYSIDDPEIQVIHQASSLTTEKEVIEWREKVIKTI
                     PLGLTKNDVPLDFQVDKEILQNPTESVIIKRGSTREFSRQSISFRQLSAIIYYSTRGI
                     PADFLKEGCSLVDLYLIVNSVDGLDSGAYYYNREQNSLQLLKAGNLRSAAGHLGLDQS
                     LPADGSVCVFFMSDLNEVLDKFGNRGYRASQLEASTIGGRFYLASYAQGLGATGLTFY
                     DDEVTQFFSPHTKNKSVMFLVAIGKKATI"
     gene            1397..2386
                     /gene="trxB"
                     /locus_tag="VGA92_02585"
     CDS             1397..2386
                     /gene="trxB"
                     /locus_tag="VGA92_02585"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9845332.1"
                     /translation="MMAATSENTIQVEKNPPKKPGKKKTKYDVIIIGAGPAGYTAGIY
                     CSRARHDTLILSGILPGGQLVNTTDVENYPGFAEGIMGPDLMIVMRKQTEKMGATIID
                     DEAVDVDFRHRPFKILTASEEYEARAVIICTGADYRKLGLEGEKTFAGKGVSYCATCD
                     GAFFRNQELIVVGGGDSAMEESTFLTKFASTVHVVHRKDTLRASKIMQERAMANPKIK
                     FHWNKEIEAISGNQKVQSVTLKDTKTGEKTTLNVGGVFVAIGHVPSSKIFQNQIELDK
                     NGYVVLKNNTETSVKGVFAAGDVHDHTYKQAVTAAGFGCMAAIDVDRYLTENS"
     gene            complement(2387..>2947)
                     /locus_tag="VGA92_02590"
     CDS             complement(2387..>2947)
                     /locus_tag="VGA92_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9845333.1"
                     /translation="PDKYFSPNQYANEANYLGHYNGTGPEIWKQTDGKITHFFTGVGT
                     GGTVTGVSAFLKEKNPKIKIIGVQPQQNHLIQGLRNFEESAKPDLFLKREYVVDDWIT
                     INNVKAFSAVKAVFAKDRLLVSPSSGAVYAAMKEYKIDSGNIVGIFADDGRKFKSLYS
                     QQKVFSEAEFDAALKKAEHMSKIVYM"
BASE COUNT          948 a          570 c          580 g          849 t
ORIGIN      
        1 aatactttgg ctctaagctc gacacataat ttgccagcca aagtaattgc agggtttgta
       61 gatgaggcag tcaatttgct tcttgacctc aacacccaaa aggaggtatc tcttgcacta
      121 gtaccaattg gatttacaaa agaaattcct accgaaaaat tcttagtgca aaagctaaac
      181 ctggcaaccg agtcgctttc aagctatagt attgatgatc ctgaaattca agtcatacat
      241 caggcttcct cactaaccac agaaaaagag gtcattgaat ggagagaaaa agttatcaaa
      301 actattccac tgggcttgac aaaaaatgat gtcccattag actttcaagt ggacaaggaa
      361 attttgcaaa atccaacaga atcagtgata atcaaacgtg gttcgacaag agagttttca
      421 cgccaatcaa tctcatttcg ccaattatct gccatcattt attattcgac aagaggaata
      481 cctgcagatt ttctaaaaga gggatgctcc ttagttgatt tgtatttgat cgtaaattca
      541 gtggacggac tagattctgg cgcctattat tataacagag aacaaaactc acttcaactg
      601 ctcaaagcgg gaaatctaag aagcgctgca ggccacttgg gactggatca aagccttcct
      661 gcagatggaa gcgtatgtgt tttctttatg agtgatttga atgaggttct tgataaattt
      721 ggaaatcggg gatacagggc atcacaactg gaagcaagca caataggagg aagattctat
      781 cttgcttctt acgcacaagg actaggtgca acaggtctta ccttttatga cgatgaggta
      841 acccagtttt tttcaccaca tacaaaaaac aaaagtgtaa tgtttttggt tgcaataggt
      901 aaaaaggcaa caatttagta tctcttttta gtttctcctt tctttatcat ccaccaccga
      961 aagtaaacaa gaaaagacaa ccctgcaatg atgcttgcta tgagcacgta ttggcctgta
     1021 tggtcttgaa aaatgcattg ctgaatctga ctgtatagtt cgtagtactc taaaagcgtg
     1081 ggatcaccat agctgtccag attctgttca ttgagaaaat tgattctctc ttgaatttga
     1141 ttttgcaact cttcaagatt ttccttatct tgtgagctgc aatcatagcg tatttgtaga
     1201 aagagttttc ctagattaat caagatttac tagagattag ttttctttac atttagtatt
     1261 gttggttact ggtttttcga tcctgctaat ttttctataa tctttgatac aaaactgtcc
     1321 tatcttacaa aaacttgaga ttcaacacag ccatatcaaa gatggacttt aatattgaat
     1381 taattgattt tattctatga tggcagcaac ttcagaaaat accatacaag ttgaaaaaaa
     1441 cccaccaaaa aaaccaggca aaaaaaagac aaaatatgat gtaataatta ttggcgcagg
     1501 accagctgga tatacagcgg gaatttactg ctcaagagct agacatgaca ctctaatcct
     1561 atcaggcatc ttacctggag gtcagctagt aaacacaact gacgttgaaa actatccagg
     1621 ctttgcagag ggaatcatgg gtcctgatct tatgatagtt atgagaaaac agacagagaa
     1681 aatgggtgca acaataattg atgatgaggc agtagacgtt gattttagac acaggccatt
     1741 caagatcctt actgcatcag aagaatacga ggcaagagca gtaattatct gcacgggtgc
     1801 agactataga aaacttggcc ttgaaggcga aaagaccttt gcaggaaagg gagtatcata
     1861 ctgtgcaaca tgtgatggtg ctttttttag aaaccaagag ctaattgtag ttggcggagg
     1921 cgactctgca atggaggaat ccacctttct tacaaaattt gcatcaactg tccatgtggt
     1981 tcacagaaaa gatactctaa gggcaagcaa aatcatgcaa gagcgtgcca tggcaaaccc
     2041 caaaataaaa tttcactgga acaaggaaat tgaggcaatc tcaggaaacc aaaaagtaca
     2101 aagtgtcaca ttaaaggata caaaaactgg tgaaaaaact acgctaaatg ttggtggcgt
     2161 ttttgtagca atcggccatg tgccaagctc aaagattttc caaaaccaaa tcgagcttga
     2221 caaaaatgga tatgttgtat taaaaaataa cacagagact agtgtcaaag gagtctttgc
     2281 agctggtgat gtccatgacc acacatacaa gcaggctgtt actgctgcag gatttggctg
     2341 catggcagca atcgatgtag acaggtacct aacagaaaat agctaattac atgtacacaa
     2401 tttttgacat gtgctctgcc tttttgagtg ctgcatcaaa ctctgcttcg ctaaacactt
     2461 tttgctgaga atacaaactc ttgaatttgc gtccatcgtc tgcaaatatt ccaactatat
     2521 tcccagaatc aattttgtat tctttcattg cagcatagac tgcaccagat gatgggctaa
     2581 caagcagtct gtcttttgca aaaaccgctt ttactgcaga aaatgccttt acattgttta
     2641 ttgtaatcca gtcatcaact acatattcac gtttcaaaaa cagatcaggt tttgcagatt
     2701 cctcaaagtt tcgaaggccc tgaatcaggt ggttttgctg cggctgaaca ccaattatct
     2761 ttatttttgg atttttttct ttcaagaacg cagaaacgcc agttactgta ccgcctgttc
     2821 caacacctgt aaaaaagtga gttatcttgc catctgtttg cttccagatt tctggccctg
     2881 tcccattgta gtggccaagg tagtttgcct catttgcata ctggtttggc gaaaaatact
     2941 tgtcagg
//
LOCUS       DASWKH010000034         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3048712, whole genome shotgun sequence.
ACCESSION   DASWKH010000034 DASWKH010000000
VERSION     DASWKH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048712"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1039)
                     /locus_tag="VGA92_02595"
     CDS             complement(131..1039)
                     /locus_tag="VGA92_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX9845334.1"
                     /translation="MVMHVFNAERSCKRCKGMMVTDTATGERFCGNCGYVISEQIEDS
                     GPERHFSKDERDDKSRSGIPTSLAMHDMGLATIIGAINKDATGKPLSTNMKHNMKRLR
                     TWDSRSQMHEQIDRNLRHAFTQLDKLKDKLALSDAVVEKAAYIYRKALAKSLVRGRSI
                     EGVLAAAVYAACRDAETPRTLNDVSDAINVKRKDLSKNYRLLVCELDLRIPVVNSINC
                     MSKIASKVGLSEKVKRGALLILQDANDRRITAGKDPMGMAASALYISCMKYNADVSQK
                     EIAMAAGVTEVTIRNRYKDLRKSLNL"
     gene            1232..>1773
                     /locus_tag="VGA92_02600"
     CDS             1232..>1773
                     /locus_tag="VGA92_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845335.1"
                     /translation="MVVGALENTLYSRQVCLVLVGKVPLIACHSLSKCTDTSFFHMTC
                     FGTKPVPIRTIGFVRVDYQQLIVIKREKCVPKSRQDHKSLVKKLIDHFVSSGLEIQYA
                     NYDGYDKPFVITRHAPDVIAFDRTKQLGYIGEAEMCNEITEQLTKEKFEDFSKKLMRK
                     GKSEHTRLPFFIASPHECNS"
BASE COUNT          500 a          385 c          349 g          539 t
ORIGIN      
        1 aggcgaagag ccagacaaaa tctacacgta tgacaagaac ggcataagaa ccgaagtcaa
       61 gccataaact tttgcaccat attcacaaaa atagtaatca ccatacaatg gttgattcat
      121 tatgaatcga ctagaggttt agcgattttc ttaggtcttt gtacctgttt cgtattgtaa
      181 cttcagttac acctgctgcc atggcgattt ctttttgcga aacatctgca ttgtatttca
      241 tacatgaaat gtatagtgca gatgctgcca tacccatcgg gtcctttcct gcggtaattc
      301 ttctgtcgtt tgcatcctgc aagatcagta gtgccccacg ctttaccttt tcactcagcc
      361 cgactttgct cgctattttt gacatgcagt ttatggaatt tacaactgga attctaagat
      421 caagctcgca cacaagcagt ctgtaatttt ttgagaggtc ttttcttttt acgttgattg
      481 cgtctgaaac atcgtttaat gttcttggcg tctcagcatc cctgcaggct gcatagactg
      541 cggctgctag gaccccttcg attgacctgc ccctgacaag gctctttgcc aaagcctttc
      601 ggtagatgta ggctgctttc tcaactacgg catcagataa cgcaagcttg tcctttagtt
      661 tgtccagctg ggtgaaggcg tgccttaggt ttctgtctat ttgctcgtgc atctggcttc
      721 ggctatccca agtcctcaat cgcttcatgt tgtgtttcat gtttgtagaa agcggctttc
      781 ctgttgcatc tttgttgatt gcgccaatta ttgtggcaag acccatgtca tgcattgcaa
      841 gcgaagttgg aatcccagac ctgcttttat catcacgttc atcctttgaa aaatggcgct
      901 caggaccaga atcctcaatc tgctcagaaa taacataacc gcagtttcca cagaatcgtt
      961 cacctgtggc agtatcggtg accatcattc ccttacatcg cttacagctt ctttcagcat
     1021 taaatacatg cataaccata cagatcagac acaccttgta gcttattcga taataataac
     1081 tagtggattt ccccttccgt gataccgaaa ttcacacagc aaatttgcaa aaatagagtt
     1141 tgtcgataaa aaaaatgaaa aaagaagttg ttttggcaag aaattaaaat taattaaaag
     1201 cttagtttag cttaaaatct tctttgaaat cttggttgtt ggtgctttgg aaaacactct
     1261 ttacagtaga caggtctgtc ttgttttggt tggaaaggta cctctgattg cttgccacag
     1321 tctgagcaag tgcacggata catctttctt tcatatgaca tgttttggca ccaagccggt
     1381 cccaataaga accattggat ttgtacgtgt tgattaccaa caattaatag taataaagcg
     1441 agaaaagtgc gtgccaaaat caagacaaga tcacaaatct ctagtcaaaa aactaattga
     1501 tcattttgtc tctagcggcc ttgagatcca gtatgcaaat tatgatggat acgacaagcc
     1561 gtttgtaatt actcgacatg caccagatgt gattgcgttt gatcgtacaa aacagcttgg
     1621 ctatattgga gaggcagaaa tgtgcaacga gattacagag cagctaacaa aggaaaaatt
     1681 tgaggatttt tccaaaaaac ttatgagaaa aggcaagtcc gagcacacaa gacttccgtt
     1741 tttcatagca tccccgcacg aatgtaattc cag
//
LOCUS       DASWKH010000035         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3395980, whole genome shotgun sequence.
ACCESSION   DASWKH010000035 DASWKH010000000
VERSION     DASWKH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3395980"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..386)
                     /locus_tag="VGA92_02605"
     CDS             complement(150..386)
                     /locus_tag="VGA92_02605"
                     /inference="COORDINATES: protein motif:HMM:NF021921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9845336.1"
                     /translation="MVFDKTTNTIQSIKITGDFFLHPEEKIDLIESELVGTKLEKNQI
                     KEKIESLLSGCEFYGFSIDSLSDAIIKCKEANSH"
     gene            complement(458..1864)
                     /gene="gcvPB"
                     /locus_tag="VGA92_02610"
     CDS             complement(458..1864)
                     /gene="gcvPB"
                     /locus_tag="VGA92_02610"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9845337.1"
                     /translation="MKLIFEYDQQPTEHVEQIQVQLDLPKELIRDNIPIPNVNEIDVV
                     RHYTKLSKKNFGIDNNLYPLGSCTMKYNPKVSEEIARLKGFASIHPRSPAEYSQGSLE
                     VIWELQEALKEITGMDDVSLQPAAGSQCELLGIMIAKRHFENKNEKRTKVIIPDSSHG
                     TNPASVAMCKLETITVNSDSRGNMDFNEVKKQIGPDVALVMVTNPNTLGLYEENLLEI
                     KDMAHENGTLMYCDGANMNALLGISRPKEQGFDMMHLNLHKTFATPHGGGGPGAGALA
                     VRSFLSDYLPVPRITKESGGFNLDYKKEKSVGKVHSFYGNFGILVRALCYIRIYGNQI
                     RSVSENAVLNANYLFAKIRDHYHVPYDRRCAHEFVVSAKNIPNGGTIDIAKRILDYGF
                     HPPTIYFPLIVDEALMIEPTETESKETLDKYAEVLVKIASELREKPEIVKEAPHTDPG
                     RLDEVKAARQLNLRWFPQ"
     gene            complement(1861..>2188)
                     /locus_tag="VGA92_02615"
     CDS             complement(1861..>2188)
                     /locus_tag="VGA92_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845338.1"
                     /translation="NQALLALAATIYLATVGRKGLVDVAKNSYYKAHLLSEKLKKTGF
                     KALNEKYFYNEFCVKTPRSASEIFSHLLEQKIIGGLVVDDERMIICCTEKNSTVDIDS
                     YLEALK"
BASE COUNT          591 a          431 c          375 g          791 t
ORIGIN      
        1 gaaaatatcc tattgagaca catggatttg accatccata aatcctaaaa attggcgtat
       61 ctgtcacatg agtcataaga tattcatcaa ttgccatatt cattgggccg ctgttgttgt
      121 tagtttccaa aatcttgact tcgttgaatt taatgggaat ttgcctcctt gcattttatt
      181 attgcatctg atagcgaatc tatgctaaac ccgtaaaact cgcaccctga taggagtgat
      241 tcaatcttct cctttatctg atttttttca agttttgttc ccacaagttc tgattcgatt
      301 aaatcaattt tctcttcagg atgcaaaaaa aagtctccag taattttgat tgattgtatt
      361 gtgtttgtag ttttgtcaaa aaccaaatca acacatagca tcttttcgaa tttaaaatac
      421 ctcttgacag atgcagaatt tgcgttgttc tgattattta ttgaggaaac catctcaagt
      481 ttagttgtct tgccgccttg acctcatcaa gtctgcctgg atcagtatgt ggggcctctt
      541 ttacaatttc aggtttttct cttagctcag atgctatttt tacaagaacc tctgcatact
      601 tgtctaatgt ctccttgctt tcagtttcgg ttggctctat cataagtgcc tcgtcaacta
      661 ttaaaggaaa gtatatcgtg ggtggatgaa aaccgtaatc taaaattctt ttcgcaatgt
      721 caattgttcc accatttgga atgttttttg cagatacgac aaattcgtgt gcacatcttc
      781 tgtcgtatgg aacatgataa tggtcacgaa tttttgcaaa aagatagtta gcatttagta
      841 cggcattttc tgaaacactc cttatttgat ttccatatat ccttatgtag caaagtgcac
      901 gaaccaaaat tccaaaattt ccataaaatg agtgaacctt gcctactgac ttttcttttt
      961 tataatctag attaaaccca ccactttctt ttgttattcg tggtactggt agataatctg
     1021 acaaaaatga tcttactgca agtgcaccag ctccaggacc acccccaccg tggggagttg
     1081 caaaggtttt gtgtaaatta agatgcatca tgtcaaagcc ttgttctttt ggtctgctta
     1141 tcccaagaag tgcattcatg tttgcaccgt cacagtacat caatgttcca ttttcgtgtg
     1201 ccatatcctt tatctccaag agattttctt cgtaaagacc aagcgtgttt ggatttgtta
     1261 ccatgacaag ggcaacgtca ggaccaatct gcttttttac ttcgttaaag tccatgtttc
     1321 ctctggagtc tgaatttact gtgattgttt ctaatttgca catagcaacg cttgcaggat
     1381 tcgtaccatg agacgaatct ggaatgataa ccttggttct tttttcattt ttattttcaa
     1441 aatgtctttt tgctatcata atccctaaaa gctcacactg agatcctgcg gcaggttgaa
     1501 gtgagacatc gtccatgcct gtaatttcct ttaatgcctc ctgtaattcc cagattacct
     1561 ctaaggatcc ttgagaatat tcggcaggag agcgtggatg aattgatgca aatcctttga
     1621 gtcttgctat ctcttcactt acctttggat tgtatttcat tgtacaggag cccaaaggat
     1681 ataggttgtt gtctattcca aaatttttct ttgataattt ggtataatgt cgtacaacat
     1741 ctatctcatt tacgtttgga atgggaatat tgtctcgtat cagttccttt ggcaaatcaa
     1801 gttgtacttg tatctgctct acatgttctg ttggttgttg atcatattca aagatgagtt
     1861 tcattttaat gcctccagat aagaatcaat atctactgta gaattttttt ctgtgcaaca
     1921 tatgatcatt ctctcatcat caactactaa tccaccaata atcttttgtt caagaagatg
     1981 tgagaaaatt tcacttgctg atcttggagt ttttacacaa aactcgttgt aaaaatattt
     2041 ttcattcaaa gccttaaagc cagttttttt cagtttttca gaaagcaaat gtgccttata
     2101 gtaggaattt tttgccacat caacaagccc ttttcttcct actgttgcca agtaaatggt
     2161 tgcagcaagt gcaagcagcg cctgattt
//
LOCUS       DASWKH010000036        24827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_82336, whole genome shotgun sequence.
ACCESSION   DASWKH010000036 DASWKH010000000
VERSION     DASWKH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 24827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24827
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82336"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VGA92_02620"
     CDS             <1..353
                     /locus_tag="VGA92_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845339.1"
                     /translation="IKLDFNEVERRTLDMNPLFQLSTRNYDEEIPLIRKAMVELENIC
                     KVKNGYKIGEPFTRAGWTFFDLQISSEMASIIEKSGMMEGALGYRIAEQLKNFLGHHI
                     EKYGSNVRITKIDY"
     gene            complement(350..1393)
                     /locus_tag="VGA92_02625"
     CDS             complement(350..1393)
                     /locus_tag="VGA92_02625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9845340.1"
                     /translation="MKRNHLLAMNLAVSSLLVFLFVPVNAAQLMDKTESHIHDHLALV
                     DYKTPRAQFMDKVPVTMVTCEEGFELIMKKSNGMPACVKPLTVAILIERGWGIHVLPD
                     YENGDNNSDMLKTEKGEFAIKTETVTYFENYTGYLAMPDIDEENLPGVILIHEVWGLN
                     DNMKEMAEELASHGYVALAVNLYGADAATTIDEGRQLMSSYDPEKGVDSMSGAVDYLQ
                     ANYGVQKVGSIGWCFGGGQSLELALNNSDMDATVIYYGRLTTDPERLSSISWPVLGIF
                     AGLDTGITPESVNEFEKALDELGIQNEIHIYPNVNHAFANPSGDRYAPEETKDAWAKT
                     LAFFEATLKNTSG"
     gene            1493..1690
                     /locus_tag="VGA92_02630"
     CDS             1493..1690
                     /locus_tag="VGA92_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845341.1"
                     /translation="MMTRIYMITFVAIVAVLAVAVSPITTAFAQGSSDGNNPQTHSED
                     KTYDDKDGKSCPGKYKGEMST"
     gene            complement(1794..2153)
                     /locus_tag="VGA92_02635"
     CDS             complement(1794..2153)
                     /locus_tag="VGA92_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX9845342.1"
                     /translation="MTKYVDGYVLPIPKKQLQAYRRMAQKAGKVWRKYGALEYFECVG
                     DDLNSKWAGIKFPKTVRAKPSEKVVFSFIVFKSKTHRNQVNAKVMNDLMKEFKDKPMP
                     FDVKRMVYGGFKVLVDV"
     gene            complement(2266..3003)
                     /locus_tag="VGA92_02640"
     CDS             complement(2266..3003)
                     /locus_tag="VGA92_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9845343.1"
                     /translation="MDLTDIIFFVVGFISEVVGTMAGFGSSTIFLPLASYFVDFKTAL
                     VLVAIFHLFGNISRITFFRHGLDKRVLLVFGLPSFALSLIGATLVGDLSQTILKLILG
                     VFLISLSLTFLLRPKLAFPSNTKSLVLGGGISGFIVGLVGTGGALRATFLTGLSISKE
                     RYIATAAVIALCTDATRIPSYVAQGFLTEQYYYYIPILFAIAISGSFVGRKLVSRINQ
                     EKFKKLVLIAVILASIKFIIDGIVGLA"
     gene            complement(3009..3320)
                     /locus_tag="VGA92_02645"
     CDS             complement(3009..3320)
                     /locus_tag="VGA92_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9845344.1"
                     /translation="MDISREFLLGFIKIHLLYHANKEGFCGIDMMDELKRHGYRIGPS
                     TLYPTLHKLQESGYLRSEKRNESGKMRIYYRATSKGKLALSRIKPKIDELVSEITENN
                     K"
     gene            complement(3471..3544)
                     /locus_tag="VGA92_02650"
     tRNA            complement(3471..3544)
                     /locus_tag="VGA92_02650"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3508..3510),aa:Ile,seq:cat)
     gene            complement(3576..4223)
                     /locus_tag="VGA92_02655"
     CDS             complement(3576..4223)
                     /locus_tag="VGA92_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX9845345.1"
                     /translation="MNSLVKKIDELIEQKSLLKHPFYQMWRDGKLSMDSLAGYSKEYF
                     QMVKAVPSFVDAIAKFAPETMIGEIRSNGAEESEHIQPWTDFAVSLGVQRDEIDNYTG
                     LEKTNQAVSELSELMTSFEGGAAAMYAFEKEIPKISHTKLDGLEKFYNITSDDATQYF
                     RLHMEADIRHAATWQNILENIPEEKYSALIEVAEKSLSAQNHLLDSCYESYCQTL"
     gene            4304..4379
                     /locus_tag="VGA92_02660"
     tRNA            4304..4379
                     /locus_tag="VGA92_02660"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4340..4342,aa:Glu,seq:ttc)
     gene            4462..5313
                     /locus_tag="VGA92_02665"
     CDS             4462..5313
                     /locus_tag="VGA92_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9845346.1"
                     /translation="MDRKNLKINPLLETYKEYITKIDDALESELTLYSKSEFMQPLMY
                     AIEGGKRIRPLILLLAAECVGRVDENAYTAGCAVEFLHTESVIHDDIIDNETSRRHKD
                     PFHIKYGYNASILTGDFVLGLILNISSRLNNPRITRDLANTAMLMSEGEVIETRLETS
                     QDLTFDDYLKVIEYKTATAFETAARLGAILGNGSEDQIIALSEYGRNIGIAYQIRDDL
                     IDWKNEDKLFNLLVKHSLDPRDVFNHMEELLKDYAAKATSSLRILPDNDAKSHLEYLL
                     RLTTLKL"
     gene            complement(5297..6784)
                     /locus_tag="VGA92_02670"
     CDS             complement(5297..6784)
                     /locus_tag="VGA92_02670"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX9845347.1"
                     /translation="MIEITSTPIILIAILGTVGMILPIISIARKEKSSNSLYAAIAFG
                     ALLVSIGYVIYETVTNHIPQGALFSEDVLADDSFSSLFAIAMLIVAIITTVGSFNFMR
                     QHSHHAVYYSLILLSTIGMVLVAYSTDLVMLFVAWELMSIPTYVLAGFIKKNPSSNEA
                     ALKYFLFGAVSSAIIIYGISLVYGLTGTTNIGGVITGLSVLDPSMMPLALLAVGMFIA
                     GFGFKMGLVPFHMWLPDTYEGAPPTITALLAAGTKKAGFAAALRVIVMGTIALNVDWT
                     LALGVIAIMTMTVGNIAAIMQKNISRMLAYSSIGHAGYILIGLAVAPFTVLGIQGSLF
                     HVLNHAVMKGAAFIAIAGIVATLGITNIDKLKGLGRKMPITTLGLVISLLALAGIPPT
                     AGFWSKLILFGAALDPSVPAWAAWLAIAGVLNSALSLAYYGWLIRKMYFEGETEKRIA
                     EPKAIIGVMVFSIIFLVAIGVYPDPIIEFTRNAVPILGATLTASG"
     gene            complement(6795..8861)
                     /locus_tag="VGA92_02675"
     CDS             complement(6795..8861)
                     /locus_tag="VGA92_02675"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9845348.1"
                     /translation="MLNFGFEASGINAWLVWILPFVAALIIPAVGKFSKRGTGWVAVG
                     LAAVSAISASMLLPGALEAQEIHEQVNWISSIGIKAGVLADPLAVIMANVVGWISFLI
                     MVYSTGYMKGDRDIVRFWFWMMFFVGSMQLIVLSDNLLMMFFGWEGVGLASYALIGFW
                     YRDKKKDYVGVHGKTVLGIADYYSPTHSGMKAFIMTKVGDIMMLAGMFLIFAFAGTFG
                     FRELLQDTAWASSMQAQGLLVPAAVLLFGGAIGKSAQFPLNEWLLEAMTGPTAVSALI
                     HAATMVKAGVFLVARLGPLFFALGAAGFAVDQFFEIIAWVGGITALLLATQGMVNPEI
                     KKVLAYSTGSQIGYMMMALGVAGLSHQFVDGYTAGFFHLMSHAMFKASLFMAAGSLLH
                     VVGSRFMTDMGGLRKHMRKTYAFMWAAGLGLMGAPFITTGFWSKDAIFAAVFESGNTW
                     AMPLFIIAVVTAVITAFYTTRMIGMVFFGDKSKHIEHAEKEGRHIHEAPMSMWVPYGI
                     LAAMTIGIGVIGLSAENGIHELFTEYLATTFHIESAHEVMESTGLPSFLEGINPVALA
                     ASLAAFGIGISLGYVFYIGRFVDPVKFVNSNIFFYSIHKVILNRWYLNAIIYWCFVVA
                     PLWIARGVWRYFERTAIDYGMNVGIERAVGWGAKVVQATQTGVAQSYLYVFGAGILFV
                     VLFLFI"
     gene            complement(8876..10450)
                     /locus_tag="VGA92_02680"
     CDS             complement(8876..10450)
                     /locus_tag="VGA92_02680"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9845349.1"
                     /translation="MEFALLQAVFLPLLLAPVAYIIGRKMGPTPATWFTFGLLLYCTV
                     LIIYAALQGTYEEHYQWTSLFGEFGFMLDGLAVPFAIIIYVLSTILVLYSQPYMIHKF
                     KEFYEEHAHGHTTDSTGTQTTALVESAGLTNYVNNQSGLYFALYLVFAMGMLGTVLAT
                     NLIEFYIFFEVMLIPAFFLVAFWGDGPRRRISLMFLFWTHVGAVVLLLGFLAIGLSVG
                     SFDFAAIQESKIPPDIVTIAAVAIILGLGVKLAAFMFHIWLPYVHGAAPTPISALLSP
                     AMIGIGAYGIFRLVVEFLPNTYSELAIWLHIWGIATMIYGGAMALMQDDIKRLLAYSS
                     VSQMGYILFGIGSYSTLGLAGAEFMYVTHGLGKALLFMTAGILIVQCGTRSLTKLGGL
                     AGKMPITAVCAVIGVLTIAGVPPTSGFMGEWTLFAGALDTAIKENAADGAILRYVVFG
                     LGLVATVLTMSYLLWMLKRVFFGKLPEHLSHVKEASWYMTAPMMVVAGFTIVVGIYPD
                     IFFDKIIPYMRGVLGA"
     gene            complement(10450..10755)
                     /gene="nuoK"
                     /locus_tag="VGA92_02685"
     CDS             complement(10450..10755)
                     /gene="nuoK"
                     /locus_tag="VGA92_02685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9845350.1"
                     /translation="MSNTILDFVIVSIALLAIGIYGVSVKRNAIRMLFAIEIIVNAAN
                     LNLVAFARFLPNSQGQTFALFSIAIAAAEVGVGLALIIVAYRMYKNVDIAEFRSLKG"
     gene            complement(10736..11248)
                     /locus_tag="VGA92_02690"
     CDS             complement(10736..11248)
                     /locus_tag="VGA92_02690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9845351.1"
                     /translation="MADAVFLALAVMTIGSAIAALEVRSLIYGSIALMGTLGGIAGFF
                     LLLDSPFVAMFQIAVYIGSIAVLILFTVMLVRRQLIFIKIEDPRRRYAGIGLMLIMMV
                     ALGAVILSSGIKSITTDEPPVDFRAIGEDFLIYYWPALILMGLILASAVIGALTLARR
                     EDVTNEQHNT"
     gene            complement(11241..11738)
                     /locus_tag="VGA92_02695"
     CDS             complement(11241..11738)
                     /locus_tag="VGA92_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX9845352.1"
                     /translation="MGTATGIIKALNSGIKHLAVKRFTLRYPEQKLKLIGDGYQYDPT
                     SGVGIAGYKGRHMLFHDKCTGCQLCAIACEGVAEAIAMVKVQEDWKQNKKAIMPQIDY
                     GKCVFCGLCVDACPFYALYMTNDYELSSFTKEGLIYTPAQLQVKPEVQQDAEIVFDKR
                     GATHG"
     gene            complement(11738..13039)
                     /gene="nuoH"
                     /locus_tag="VGA92_02700"
     CDS             complement(11738..13039)
                     /gene="nuoH"
                     /locus_tag="VGA92_02700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX9845353.1"
                     /translation="MSALAPKFRFSYFIKSILDNLFWIIILLTLTGIPLVMILLFYLE
                     LPVIGGKLLDPYLAFTTLADPSRQIPLLKSLLQTEFFRIAVFPGFGFAALIAAGTIFV
                     ERKMLAKLQLRVGPFYCGKVEGILQLMSDGIKLLSKEIIIPAKADKPIFWAAPVLFVA
                     TAAAFIALIPAAGGGWVVADVDVGLIAVFAVIGFFPVITVLSSWSANSKFPFIGGIRA
                     LHQMVSFEIPLILSGLGVVILTGTLNLTEIVESQDPYWWIIFLPIGAIVFFIAMLAEL
                     ERIPFDLPEAESEIVAGWLTEFSGMMYGLVQLGSYLKLYAFAALFVVLFLGGWSGPQI
                     WPPFPEEIITEGIQMGPINAQFPGLPLLDQQMLNDVLWFVVKVVGVIFFILLPRGVFP
                     RVRIDLLLHIGWYKLIGLAFVNIFIALGLLYAGVLGPEGIL"
     gene            complement(13040..14173)
                     /locus_tag="VGA92_02705"
     CDS             complement(13040..14173)
                     /locus_tag="VGA92_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9845354.1"
                     /translation="MTTQLPPGLQIEKVDEKIMTLNVGPQHPGSGHMRLIVKVDGDYI
                     VSCDPDPGYVHRGEEKMAEHRNYITNIPHLERPVIHDSCNILYPYCLGVEELLGLEVP
                     ERAKYIRVIAAELNRCVYTQYWLAIYGIFLGHSTMFMWPAGDRELLIDALEKMTGARV
                     THAYFVPGGIRNDVPANFEEMCLRRVNYFEKRIKEYADIFYDNPILISRTEGSGVLSR
                     NDAIRLGTTGSTLRASGVDFDVRTKEPYDVYDELDIKTNVLKAGDSYARSKIPWLDML
                     ESCNIIRQALQKMPKSGSVRVKLKPNPKGGNDEVYKRVESGRGALGCYIVSRSKPEPY
                     RLKMSVGSFRNLICLPYLLKGEKLGNMPAVYWSLNYWPVEADR"
     gene            complement(14175..14723)
                     /locus_tag="VGA92_02710"
     CDS             complement(14175..14723)
                     /locus_tag="VGA92_02710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9845355.1"
                     /translation="MSTTPEQTKPVAKAETKELPKFEKSITDKLEAKFASKVAIEFVK
                     PTRIRVKVAKEDILDVAKFIRDELHFDHAESVTGVDYPDKKEIEVVYHLGSYTDPELA
                     KQILALATGVPREDDPNPGNDSTKLASLRDIFYSVEFHERECFEMLGVYFEGHPDNRR
                     LLLPEDWSDLPPLRKDFKLKGR"
     gene            complement(14733..15248)
                     /gene="nuoB"
                     /locus_tag="VGA92_02715"
     CDS             complement(14733..15248)
                     /gene="nuoB"
                     /locus_tag="VGA92_02715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX9845356.1"
                     /translation="MLKDLLTPYNANVFVGKLGDVLVKAIGAPLGYAINWSRLWSLWP
                     VHIETACCSVEFGAASGPRYDVERFGIIEAFGSLRQCDLVVVQGTITRKMAPRLRLVY
                     DQMPEPKFVIAMGACAITGGLYFDSYNVLPGIDGVLPVDVYVPGCPPRPETLIQGCML
                     LQEKIKRMKAR"
     gene            complement(15291..15623)
                     /locus_tag="VGA92_02720"
     CDS             complement(15291..15623)
                     /locus_tag="VGA92_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9845357.1"
                     /translation="MFGFAIVAVGPALLISRMISPRRHSNPVKFLPMESGQVPKGEGR
                     THFMMQYYAYVLMFVIFDVMAIFLYAWGSSILSLPKTATLPIIAFLGIMFAAMAFALY
                     QSKRRDIW"
     gene            15781..16254
                     /locus_tag="VGA92_02725"
     CDS             15781..16254
                     /locus_tag="VGA92_02725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9845358.1"
                     /translation="MAVNVGDKAPEFELVDTELKMRKLGDFKGKKVVLSFFVAASSPV
                     CTNEMCTFRDRWSEISNLGAQVIGISNDGPFANKAFAEKFNLKFPILGDYKSKTIRDY
                     DILMPDLLHVKGYDAAKRSVFVVDEDGKVVYRWVSDNPLVEPNYQEIIDFLKKEK"
     gene            complement(16267..16779)
                     /locus_tag="VGA92_02730"
     CDS             complement(16267..16779)
                     /locus_tag="VGA92_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX9845359.1"
                     /translation="MEYKIEDVEGTFNAQILKNLGNNEYVIKIGTQEHQLKILHMDSR
                     GIEFILDQKYHKAKYLESATAQMNLVVDGIPMKLNPNSKFDEIVYKNTGGGGSVDAQI
                     SLRSQIPGKVVSVNVTEGDSVKKGDVVCVLESMKMQVSIKSHKDGVIKKLKIKSGASV
                     AKNDVLAEIE"
     gene            complement(16784..18271)
                     /locus_tag="VGA92_02735"
     CDS             complement(16784..18271)
                     /locus_tag="VGA92_02735"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9845360.1"
                     /translation="MIEKILISNRGEIALRVIRTCKALGIKTVAVYSDEDYNSLHVKQ
                     ANEAYHIGKAAPKESYLNQEKIMEVILASGADAVHPGYGFLSENSDFAKLCEDNKINF
                     IGPSSASMDLCGDKMRCKAAMLKAKVPTVPGSPDLVKDVEEALSIANKIGYPVLLKSV
                     YGGGGRGIRLVNNDKELREAYETVTGESIAAFGKSAILVEKFLEKIRHIEYQLARDKH
                     GNTIHIFERECSIQRRNQKLIEQTPSPAVDQKTRDRIGELVISAANAVGYTNLGTAEF
                     LRADNGEFYFIEINARLQVEHPITELVSGLDLVKLQIDIANGEPIPFKQKDLKMNGYA
                     IECRINAEDTFLDFAPSTGPVPDVTIPSGPGVRCDTYLYPGCTVSPFYDSLMAKLVTW
                     GQNFEESRLRMLNALNDFYIQGVETSIPLYKTILKTEEYKKGELSTDFLKRYGIIEKL
                     TEDVKSEKSEKKEPALAAAIMHSEYFKSRVISSANSNHSWKSKLG"
     gene            complement(18277..19824)
                     /locus_tag="VGA92_02740"
     CDS             complement(18277..19824)
                     /locus_tag="VGA92_02740"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9845361.1"
                     /translation="MHSEKIEKFLEKQNKALTAGGQDRILAQHEKGKLTARERINLLL
                     DEGSFTEIDALVTHHYYEYDMQKKKFFTDGVITGYGTINGRQVFVFAYDFTVLGGTLS
                     QMGAKKITKLMDHATRTGCPIIGIIDSGGARIQEGILSLDGFADIFYHNELASGVVPQ
                     ITASIGPSAGGAVYSPAMTDFVIMVEKAGTMYVTGPEVVKTVLGEEVSFEDLGGAITH
                     GTKSGVAHFVAKNEYDCFDKIKTLLSYLPANNTEDSPLVVSDDDPNRMDHNLINKIPE
                     NSLQPYDMKEIVLSIVDNHQFFEVHELFAQNVMVGFARMHGKTVGIVANQPLYLAGAL
                     DIDSSNKASRFIRFCDSFNIPIISLVDTPGYMPGTNQEHNGIIRHGSKLLYAYCEATV
                     PKITLVIGKAYGGAYIAMGSKYLRTDINYAWPTAQIAVLGSESAVKIMNKKDLDAAKN
                     PKELKKQLTDEFNEKFANPYVAASNGSVDAVIDPAQTRPMLIKALEMLANKRDKQLPR
                     KHGNINL"
     gene            complement(19967..20248)
                     /locus_tag="VGA92_02745"
     CDS             complement(19967..20248)
                     /locus_tag="VGA92_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845362.1"
                     /translation="MDEMTYLVPTYNPTRVLSPEFRRTSNYELGIVDLTKEKIETLER
                     LKRDPEANKVKINQAEEDLKTLGYLYENYNVGMNVFRTAKGGRDKLRTE"
     gene            complement(20368..21216)
                     /locus_tag="VGA92_02750"
     CDS             complement(20368..21216)
                     /locus_tag="VGA92_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9845363.1"
                     /translation="MDSKDTRRDRVSTIDSIIEEERKELNNIQTLIKKQSKSLERAKK
                     FLTEKQMLLQRELDKKAFKFSNNEKFTIMGELSSRMAHDIRNPLNVIKVQVDLLKLRY
                     SKQEDKIMLDSLGRMERAVNGITDQLEDILNFLKDSPMQFEGSNIMKILNDSLLNIHK
                     PDNVLVELPNCDVTVVCDSYKMQRVLTNMIQNSIHAMEDGGTIIIQIFEQNDEVKIEI
                     KDTGLGIPDEVLPRIFEPLFTTKRTGTGLGLSICRKIVEDHNGSISVKNNPTTFTITI
                     PKLQQD"
     gene            21537..21845
                     /locus_tag="VGA92_02755"
     CDS             21537..21845
                     /locus_tag="VGA92_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845364.1"
                     /translation="MLFGVFATHSPESCPLNNKDSKKIFLGIEGKLKANLKKFNISKI
                     VGFYMSVLEHQWIIILDAKTAHDIEQFCISVGISAISTVKIVPINDFAVTANKLRSQK
                     "
     gene            complement(21915..22241)
                     /locus_tag="VGA92_02760"
     CDS             complement(21915..22241)
                     /locus_tag="VGA92_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845365.1"
                     /translation="MSWRKIPMKFPGTCIVCSQKIEVNEMGLWAKGIGVKHEKCASKE
                     VKELKCIICGGPAGCQKCEFFDDCDRESVSELCICKKCSDSKDSFIAYQNAVKKNFVL
                     LNLKTD"
     gene            complement(22238..23395)
                     /locus_tag="VGA92_02765"
     CDS             complement(22238..23395)
                     /locus_tag="VGA92_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845366.1"
                     /translation="MWSEKHRPKKIIDMVGNEEARKSFVDWLVKWKKGVKPVLLVGPP
                     GIGKTTLAQLAAKEFGYDLIGLNASDVRNKANIEEILTPLLGNVTLLGKPMIFVDEVD
                     GIHGRADFGGVEALIKILKEPTVPIILAANSDQSDKMKNIKKTTKPIYLKPLPPRLLG
                     LYLQKVLKEEGATLGPGATIKIIIDSRGDIRSLLNMAQAHVTGFDPPTEKSFETLNID
                     AAVNAFFKAKSKEEAQVVLYSLRIDPREKINAFYSSIVSSNLQSKDLAKMLGVISEAD
                     MLYGKIMRTQEWRLLRYLDNILLRLYEENLPVQFSQYNLSWPLLNRLRWDGKTIKALG
                     ALLAKKFHVSKSTIATFYLPYILFCMKNKKLEMELSPEYDEILQKEIDLLR"
     gene            complement(23406..24521)
                     /locus_tag="VGA92_02770"
     CDS             complement(23406..24521)
                     /locus_tag="VGA92_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845367.1"
                     /translation="MSSKDQSVVSKEALMSTKPGKQILKQGLFKSKGFKLFNQYKEEA
                     EAEFPKFADRFTDDLLREIKSDASASSTQKEFAQEVGSTEIILQDSQIPDIKSRLENR
                     DVLKDRVLRILNSNFVKMTLPVFNALYDAASGFYGNKNAQMRQDLVDGHIIAIDLSEP
                     MDRIVDKDEDLEYLDDYKLMNPYILRLARAKIAKGGEEILREFEEGFRDARLGQYTDT
                     KLKANPTKITEEQMNQCYKKYRAVMGTAGRNMALAKNPLGEIFYLGMARAAESVGCGN
                     EIEDSIKNKFVKIPSWPLYYSLLTNNVEAGFVFTMKKSELYLSDARLALELLPKDFSH
                     TEFLEFLFLTVEHYNQYWFNQLEKANLWDEFSSKIPK"
     gene            24599..>24827
                     /locus_tag="VGA92_02775"
     CDS             24599..>24827
                     /locus_tag="VGA92_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HEX9845368.1"
                     /translation="MQPIDVLKEASKLVYKNVKDLAGTKAAAEDHGVGRGGDISRKID
                     IVAEKTVLDYLKKIKFDCIVLGEECGRVELSK"
BASE COUNT         6727 a         5561 c         5024 g         7515 t
ORIGIN      
        1 gtattaaact agattttaat gaggtggaaa gacgcacatt agatatgaat ccgttatttc
       61 agctgagcac tcgaaactat gacgaagaaa ttcccttaat tagaaaagca atggtagaac
      121 ttgagaacat ctgcaaggta aaaaatggat acaaaattgg agagccattt acaagggcag
      181 gctggacttt ttttgatctc cagataagct ccgagatggc atcgataata gaaaaatctg
      241 gaatgatgga aggggcacta ggatatagga ttgcagaaca gctgaaaaat tttctggggc
      301 accatattga aaaatatgga agcaatgtga gaattacaaa aattgattac tagcctgatg
      361 tattctttag agtcgcctca aagaaggcaa gcgtttttgc ccatgcgtcc ttggtttcct
      421 ctggtgcata tctatcccct gatggatttg caaatgcatg gtttacgttt ggatatatgt
      481 ggatctcgtt ttggattcca agctcgtcta gtgctttttc aaactcgttt accgattctg
      541 gtgtaattcc agtatcaagg cctgcaaata ttccaagcac tggccagcta attgatgaga
      601 gtctttctgg gtcagtagtt agtctgccat aatagattac agtggcatcc atatcagaat
      661 tgtttagtgc aagttcaagg gactgtccgc caccaaagca ccacccaatt gagccaactt
      721 tttgcacacc atagtttgct tgcagataat ccactgctcc actcatgcta tcaactcctt
      781 tttctggatc atacgaagac atcagctgcc ttccctcatc aattgtggta gccgcatccg
      841 ctccgtagag atttactgca agggccacat atccatgaga ggccagctcc tctgccatct
      901 ccttcatgtt gtcgttgagg ccccatactt cgtggattag tatcactccg ggaaggtttt
      961 cttcatcaat atccggcatt gcaagatatc ccgtataatt ctcaaaatat gttacagttt
     1021 ccgtcttgat ggcaaactct cctttttctg ttttgagcat atcagagttg ttgtccccat
     1081 tttcatagtc tggcaggaca tgtatgcccc agccgcgctc aattagaatt gccaccgtta
     1141 atggcttgac gcatgcaggc attccattgg attttttcat gattagctca aagccttctt
     1201 cacacgtaac catcgttact ggaactttgt ccatgaattg ggcccttggg gttttgtaat
     1261 cgactaacgc cagatgatca tgaatgtggg attctgtctt gtccattagc tgtgccgcat
     1321 tgactgggac aaacaagaat accaatagtg aagaaactgc caaattcatt gccagaagat
     1381 ggtttcgctt cattgcatgt aaaattggta aaacatgaat attaaattaa ggattggtaa
     1441 cattgttaca cattatttaa tagtgatctg agccatactg acttgagaac gcatgatgac
     1501 tagaatctac atgattacat ttgttgccat agtagctgtg cttgcagttg cagtatcccc
     1561 cattactacc gcatttgcac aaggttcttc agatggcaac aacccccaaa cccattccga
     1621 agacaagacc tatgatgata aagacggcaa gtcttgcccc ggcaagtaca agggcgaaat
     1681 gagcacctag gcaacatcaa aaaacttctt tttttctaaa ttaaattccg gcatatcaaa
     1741 atttgattgt cagtgaaaaa aggaatttaa gattcgagaa acggtacacg gattcacacg
     1801 tcgactagaa ctttgaatcc accgtagacc atgcgcttta cgtcaaatgg catcggcttg
     1861 tccttgaatt ccttcatcaa gtcattcatt accttagcat tgacttgatt tcgatgcgtc
     1921 tttgacttga atacaataaa tgagaacact actttctcac tgggctttgc tctgactgtt
     1981 tttgggaatt tgataccggc ccactttgaa ttgaggtcgt cacctacgca ctcaaaatac
     2041 tcaagggccc catactttcg ccatactttt ccggccttct gagccatgcg gcggtaggct
     2101 tgtagttgtt tttttggtat tggcagcaca tatccatcaa catatttggt catgcaaggt
     2161 ctcctctcgt tttatgacgt tagctgaaat atttagcttt tctgctgccc atcggtgggc
     2221 caacaataca catcaaattt tttgtcatat tgagagttgt tatttttatg ccagaccaac
     2281 tatgccatcg ataatgaact tgatgctagc aagaattaca gctattagta ctagtttctt
     2341 gaatttttct tgatttattc tgctaacgag cttacggcct acaaatgagc cagatattgc
     2401 gatggcaaaa agaatcggga tgtagtaata atattgctca gtcagaaaac cctgcgccac
     2461 atacgatgga attcttgtgg catcagtgca aagtgcgatt acagcagcgg ttgcaatgta
     2521 tctttccttg ctaatgctca gcccagtgag aaatgtcgct cttaaggcac ctccagtccc
     2581 cactaagcca acgatgaaac ccgatatccc acctcccaga acaagggatt tggtatttga
     2641 tggaaaagca agtttgggcc tgagaagaaa cgtaagagaa agcgagatca aaaatacgcc
     2701 caaaattagt ttgaggattg tctgcgacag atccccaacc agagttgcgc caatcaggct
     2761 gagggcaaag ctcggtagcc caaatactag caaaactctt ttgtcaagac catgtctgaa
     2821 aaaagtaatc ctgctgatat ttccaaatag atggaatatg gctactagaa ccaacgctgt
     2881 tttaaaatcc acaaaatatg atgccagtgg caaaaagatt gtggatgagc caaaccctgc
     2941 catcgttcct acgacttccg atatgaagcc tacaacaaag aaaatgatgt ccgttagatc
     3001 cacgtttgtc acttgttatt ttctgtaatt tccgatacga gctcgtctat ctttggtttg
     3061 attcgactca gagcaagctt gccctttgat gtggctctgt agtatatcct cattttgcca
     3121 ctctcgtttc ttttttcact ccggagataa ccgctctctt gtagtttgtg aagcgttgga
     3181 tatagtgtac taggccctat gcgataacca tgtctcttta gctcatccat catatctatt
     3241 ccacaaaaac cttctttgtt tgcatgataa agaagatgaa ttttgatgaa cccaagtaaa
     3301 aattctctag atatatccat aatatcagtt tatgatatcg ttaagcgata ttataaaggt
     3361 taatggaggt tgtaaaagaa tttataatta agtgcaagat ctcatcttcc aattaaccct
     3421 cttaggactt ttgaaaagga ctcgttttaa tttaggaatg aaaaatttta tgggcccaaa
     3481 gggcttcgat cccttggctc accggttatg agccggttac tctaccaagc tgagttatgg
     3541 gccccgaaaa aagagatttc ccctaagtat aaaagttata gagtctggca gtagctttcg
     3601 taacagctat ccaaaagatg gttttgcgcc gacagggatt tttctgcaac ttcgatcaga
     3661 gctgaatatt tttcttctgg aatgttttct aggatgtttt gccatgttgc tgcgtgtcga
     3721 atgtccgctt ccatgtgcag cctaaagtat tgtgttgcgt catctgatgt gatgttgtag
     3781 aatttctcaa gaccatctag ttttgtgtgg cttatttttg gaatctcttt ttcaaacgca
     3841 tacatcgctg ctgcgccccc ctcaaatgac gtcattagtt ccgatagttc agatactgcc
     3901 tggtttgtct tttccagtcc tgtataattg tcaatttcat ctctttggac acctagcgat
     3961 actgcaaagt cagtccacgg ctggatgtgc tctgattctt ctgctccgtt tgagcgtatt
     4021 tccccaatca ttgtctctgg ggcaaacttg gcaatcgcat caacaaatga gggtactgcc
     4081 tttaccatct gaaagtattc tttagagtaa ccagcaagtg agtccattga tagtttgcca
     4141 tcgcgccaca tttggtaaaa tggatgtttt agcaagcttt tctgctcgat gagctcgtca
     4201 atttttttca caagagaatt catgacatta ccacgtttgt gccaataaaa aaatctttca
     4261 cgttcaaaaa gattttaagg tctcaagacg gcgttgcaaa cgggctcctg tggtgtagtc
     4321 cggtcaagca tgtcgccctt tcaaggcgga gatcccgggt ccaaatcccg gcgggagcac
     4381 tttacactca ccaattaata accacgcaat tacacctaca aactattaat accaataaaa
     4441 aaaaggattt ttagaagttt tttggatcga aaaaatctaa aaatcaaccc actacttgaa
     4501 acatacaagg aatatatcac aaaaatagat gatgccctag agtccgagct tacactttat
     4561 tcaaaatccg agttcatgca gccgcttatg tacgcaattg agggtggaaa gcgaatcagg
     4621 ccactcatct tgcttttggc agcagaatgt gtcggaagag tagacgagaa cgcatacacc
     4681 gcaggatgtg cagtcgagtt tttgcacaca gagtccgtca tacatgatga cataatagac
     4741 aatgaaacat cgcgaaggca caaggaccca ttccacatca agtacggata caacgcaagc
     4801 attctgacgg gagattttgt tttgggcctg atactaaaca tctcatcaag gctcaacaac
     4861 cctagaatca cgcgagatct ggcaaacact gcgatgctta tgagcgaagg cgaggtaatt
     4921 gagacgcgcc ttgagacaag ccaggatttg acatttgatg attatctcaa agtaatagag
     4981 tacaagactg ccacggcatt tgagactgcg gcaagactcg gagcaatcct tgggaacggc
     5041 tcagaggacc aaataattgc actatcagaa tacggcagaa acattggaat tgcgtatcaa
     5101 atccgcgatg atctaattga ctggaaaaac gaggacaagc tcttcaattt gctagtcaag
     5161 cacagcttgg atcctcgtga cgtctttaac catatggaag agcttctcaa agattacgca
     5221 gcaaaggcaa catctagtct gagaattctg ccagacaacg atgcaaaatc acatttagaa
     5281 tatctgctaa ggcttactac cctgaagctg taagcgttgc gccaagtatt ggcacagcat
     5341 tccttgtaaa ttcaattatc ggatctggat agacaccgat tgccaccaga aatattattg
     5401 aaaataccat tacaccaatt attgcctttg gctcggcgat tcgtttttct gtctctccct
     5461 caaagtacat ctttctaatc agccaaccat agtaagctaa agagagcgcc gagttgagaa
     5521 cgcctgctat tgcaagccat gctgcccagg ccggaacact cggatcaagt gcagcgccaa
     5581 ataggataag cttactccaa aatccagccg ttggaggaat tccagcaagt gccaataacg
     5641 atatcacaag gccaagcgtt gtgattggca tctttctgcc aagccccttt agcttgtcga
     5701 tgtttgtaat cccaagtgtt gccacaatgc ctgcaatggc aatgaatgcg gcgcccttca
     5761 ttactgcgtg gtttagcaca tggaacaatg atccctgtat tccaagcacc gtaaatggcg
     5821 caactgcaag gccaattagg atgtagcctg catgtccaat gctggaatat gcaagcatcc
     5881 ttgagatgtt tttttgcatg attgccgcaa tgtttccaac agtcatagtc attatcgcaa
     5941 ttactcccag tgccaaagtc cagtcaacat ttagcgcaat tgttcccatg actatgactc
     6001 gcagcgcagc tgcaaatcca gcctttttgg ttccagcagc aagtagtgca gtaattgttg
     6061 gcggtgcgcc ctcgtatgta tctggaagcc acatgtggaa tggaaccaga cccatcttga
     6121 atccaaatcc tgcaatgaac attcctactg caagaagcgc caggggcatc atggaaggat
     6181 cgaggacgga gagcccggta attacgccac caatgtttgt agttccagtc aggccataca
     6241 caagtgagat tccatagatt atgattgcag acgatactgc cccaaacaaa aagtacttga
     6301 gtgctgcctc gtttgagctt ggattctttt ttataaagcc ggccagcaca tatgtcggaa
     6361 tgctcatcag ctcccatgca acaaagagca tgacaaggtc tgtcgagtat gccaccagaa
     6421 ccataccgat tgttgaaagc aaaataagcg aataatagac tgcatggtgc gagtgttgcc
     6481 gcataaagtt aaacgagccg acagtggtga ttattgccac aatcagcatt gcgattgcaa
     6541 agaggctgct aaacgaatca tcagcaagta cgtcttctga aaacagagct ccctgcggaa
     6601 tgtggtttgt tacagtctca tatatgacat agccaatcga tactagcagt gcgccaaatg
     6661 caattgccgc ataaagcgag ttggagcttt tctcttttcg cgcaatgcta attattggaa
     6721 gtatcattcc aactgttcca agtattgcaa tcagaattat tggagtcgaa gtaatctcta
     6781 tcataccata tcacctaaat gaacaaaaac aggacaacaa acagaattcc tgctccaaat
     6841 acgtacaggt atgactgggc aacacctgtc tgagttgctt gcactacttt ggctccccag
     6901 ccgactgccc gctcgatacc tacattcatc ccatagtcga ttgcggttcg ctcaaagtat
     6961 ctccacacac cccttgcaat ccatagtggc gctacaacaa agcaccagta tatgattgcg
     7021 ttgaggtacc acctgtttaa gataactttg tgaattgagt aaaagaagat attagaattt
     7081 acgaatttta ctgggtctac aaatcgccca atatagaaca cgtacccaag gcttattcct
     7141 attccaaatg ccgcaagcga ggctgccagc gctactgggt tgattccctc caagaacgac
     7201 ggaaggccag tgctttccat tacttcatgt gcagattcga tgtggaatgt ggtagcaaga
     7261 tattctgtga atagttcgtg aattccattt tctgctgaga ggcctattac tccgattcca
     7321 attgtcatgg ctgccaaaat tccgtatggc acccacatcg acattggtgc ttcgtggatg
     7381 tggcgtcctt ctttttctgc gtgttcgatg tgcttgcttt tgtctccaaa gaagaccatt
     7441 ccaatcattc gtgtagtata gaaggcagta attactgcag tcaccactgc aatgataaag
     7501 agcggcatgg cccatgtgtt gccagactca aagactgcgg caaatattgc gtccttgctc
     7561 caaaatcctg tcgtgataaa tggagcaccc atcagtccca ggcctgctgc ccacatgaat
     7621 gcgtatgtct tgcgcatatg ttttcgcaga ccgcccatgt ctgtcatgaa cctagatccc
     7681 acaacatgca gcagcgatcc tgccgccatg aagagtgatg ctttgaacat tgcatgagac
     7741 atcaggtgga aaaatccagc agtgtaaccg tcaacgaact ggtgcgagag tcctgcgaca
     7801 ccaagtgcca tcatcatgta acctatttga gagccagtcg agtatgcaag tacctttttt
     7861 atttctggat ttaccattcc ctgggttgcc aataatagtg cagtaattcc tccaacccaa
     7921 gcaattatct caaagaactg atccactgca aacccagctg caccaagtgc aaagaacagc
     7981 ggcccaagcc ttgcaaccaa gaacacgcca gccttgacca tcgttgctgc gtgaatcagg
     8041 gcagaaactg cagttgggcc agtcattgcc tcaagcagcc actcatttag tgggaattgt
     8101 gcggacttgc ctattgctcc gccaaacagc aagactgctg ctggaacaag cagcccttgg
     8161 gcttgcattg atgaagccca ggcagtatct tggagcagtt cgcgaaatcc aaacgtccca
     8221 gcaaatgcga aaatcagaaa cattccagca agcatcatga tgtctccgac tttggtcatg
     8281 atgaacgcct tcattccaga gtgcgtagga gaataatagt ccgctattcc aagtactgtt
     8341 ttgccatgta ctccgacata gtctttcttt ttgtctcggt accaaaatcc aatgagtgca
     8401 tatgacgcaa gtcctactcc ttcccaacca aagaacatca taagcaagtt gtctgagaga
     8461 actatgagct gcattgagcc aacaaagaac atcatccaga accaaaatct gacaatgtcg
     8521 cggtctcctt tcatgtagcc agtactgtac accatgatca aaaatgaaat ccagccaact
     8581 acgtttgcca tgatgactgc aagtgggtct gctagtacac ctgccttgat tccgattgag
     8641 gaaatccagt ttacctgctc gtgtatttct tgtgcctcca gtgcgccagg caacagcatt
     8701 gatgcagaaa tggcacttac tgccgctagt cccactgcca cccatccagt gcccctcttg
     8761 gagaacttgc cgacagctgg aatgattagt gctgcaacaa acggcaaaat ccagacaagc
     8821 catgcgttga ttccgcttgc ctcaaatcca aaattcaaca tgctagattc caccattatg
     8881 caccaagcac tcccctcatg tatggaataa ttttgtcaaa gaaaatgtcc gggtatattc
     8941 ccacgacaat ggtaaatcct gctacgacca tcattggggc agtcatgtac cagctagctt
     9001 cttttacatg cgagagatgt tcaggaagct tgccaaagaa gacccgcttt agcatccaga
     9061 gcaggtacga cattgtaagt actgttgcga caaggcctag tccaaatact acatatctca
     9121 gaattgcacc gtctgccgcg ttttccttta ttgctgtgtc cagcgcgcct gcaaacagtg
     9181 tccattctcc cataaatccg cttgttggcg gaacaccagc aatagttagt actccaataa
     9241 ctgcacagac tgcagtaatt ggcatcttgc ctgcaagccc tccaagcttt gtcaggcttc
     9301 tagtaccgca ctgtacgatc aaaattcctg cagtcatgaa gagcaaagcc tttccaagac
     9361 catgggtcac atacatgaac tctgcgccag caagtccaag tgtagagtac gagccaattc
     9421 caaagagaat gtatcccatc tggctgacac tagagtacgc caacaaccgc tttatgtcgt
     9481 cttgcattag tgccatcgcg ccaccgtaaa tcattgtggc aataccccag atgtgcaacc
     9541 aaattgcaag ctcagagtag gtatttggca aaaactcgac tactagtctg aaaattccat
     9601 aagccccgat tccgatcatt gcgggagaca aaagagcact aattggggtg ggggctgcac
     9661 cgtggacata gggcaaccaa atatggaaca taaacgcagc aagcttgacg ccaaggccca
     9721 aaattattgc aactgccgca atagtcacaa tgtctggcgg gatctttgat tcctggattg
     9781 cggcaaaatc aaagcttcca accgaaagac cgattgccaa aaatcccaac aaaagaacta
     9841 ctgcgccaac gtgtgtccag aaaagaaaca tcagagaaat tcttcttcgt ggcccgtcac
     9901 cccagaatgc aaccaaaaag aaagctggaa tcagcatgac ttcaaagaaa atgtaaaact
     9961 cgataagatt tgttgcaagg accgttccaa gcattcccat tgcaaacacc agatacagtg
    10021 caaagtatag gccggactgg ttgttgacat aattggtaag tcctgctgat tccacaagtg
    10081 cagttgtttg tgttccagtg ctgtctgtgg tgtgtccatg cgcgtgttct tcgtagaact
    10141 ctttgaactt gtgaatcatg tatggctgtg aatacaggac caaaattgta cttagaacgt
    10201 agattatgat tgcaaacgga actgccagcc catcaagcat aaatccaaat tcgccaaaaa
    10261 gactagtcca ctggtaatgc tcctcatagg ttccctgcag tgcggcgtaa ataatcagaa
    10321 ctgtgcagta cagcagcaat ccaaatgtga accaggttgc cggtgtcggg cccatttttc
    10381 ttccaattat gtaggccact ggtgccagta acaatggcag gaagactgcc tgaagtaatg
    10441 cgaactccac tagcccttca gactcctaaa ttctgcaatg tcaacgttct tgtacattcg
    10501 gtatgccact atgatcagtg caagtccaac tcccacttcg gcagctgcaa tagcaattga
    10561 aaatagtgca aaggtctgtc cctggctgtt tggcaaaaat cttgcaaagg caaccaagtt
    10621 caggttagct gcatttacta tgatttcaat tgcaaaaagc atacggattg catttcgttt
    10681 aactgataca ccatagattc caattgccaa aagtgcaatt gatacaatta caaagtcaag
    10741 tattgtgttg ctcatttgtt acatcctccc ttctagctag agttagtgcg ccaattacag
    10801 ctgatgccaa aataagtccc atcaggataa gggctggcca gtagtatatg agaaagtcct
    10861 cacctattgc cctaaagtca acaggtggct catcagtagt aatggattta attccagagc
    10921 tcaaaattac tgctcctaag gcaaccatca ttattagcat cagaccgatt ccggcgtacc
    10981 gccttcttgg gtcctcaatt ttgataaaga ttagctgtct tcgcacaagc atcacggtaa
    11041 acagaatcaa aacggcaata gagccgatat agactgcaat ctgaaacatt gcgacaaaag
    11101 gcgaatccaa aagcaaaaag aatcctgcta ttcctccgag tgtccccata agggcaattg
    11161 agccgtaaat tagagagcgg acctcaagtg ctgcaattgc agagccaatt gtcataacgg
    11221 caagtgccaa aaaaactgca tcagccatgg gtggcacccc tcttatcaaa gacaatctcg
    11281 gcgtcctgct gaacctccgg ctttacttgg agttgtgctg gagtgtagat gagtccttcc
    11341 ttagtaaatg acgataactc gtagtcgttt gtcatgtata gtgcatagaa tgggcatgcg
    11401 tcaacgcaca gcccgcaaaa gacacacttg ccgtagtcga tttgcggcat gattgctttt
    11461 ttgttttgtt tccagtcttc ctgcactttg accatcgcga ttgcctctgc aacgccttca
    11521 catgcgattg cgcagagctg gcatcccgtg cacttgtcgt ggaatagcat gtgccttccc
    11581 ttgtaccctg caatacccac accactggtt gggtcgtact ggtatccgtc tccgatcagt
    11641 ttgagcttct gctctggata tctgagtgtg aatcgtttga ctgcaaggtg ttttattccg
    11701 gagtttagcg ccttgattat tcctgtagct gttcccatta caaaattccc tccggaccta
    11761 gtacccctgc atacagcaat ccgagtgcta taaagatgtt aacgaatgca aggccaatca
    11821 atttgtacca tccaatgtgt aagagaagat caattctgac tcttggaaag acaccccttg
    11881 gtagcaagat aaagaagatg actccgacta ctttgactac aaaccacaat acatcgttta
    11941 gcatttgctg atccaataat ggaaggcctg ggaattgggc gtttattggg cccatttgga
    12001 ttccttctgt gattatttct tctgggaatg gtggccagat ctgcggaccg ctccacccgc
    12061 ctaagaacaa cactacaaac aacgcggcaa aggcgtagag tttgaggtat gagcccagct
    12121 gcactagccc gtacatcatg cctgagaatt cggttagcca gcctgccacg atttcactct
    12181 ctgcttctgg tagatcaaat ggaattcttt ctagttctgc gagcattgca ataaaaaaca
    12241 caattgcgcc aattggcaaa aatataatcc accagtacgg gtcctgactt tcaacaattt
    12301 ccgtaaggtt tagcgtacca gtcaaaatca ctactccaag acctgataag atgagtggga
    12361 tctcaaatga gaccatttgg tgtagtgcgc ggattccgcc aatgaatggg aatttagagt
    12421 ttgcagacca cgatgataac acggtaatta ccggaaagaa accaattact gcaaagactg
    12481 caattagtcc tacatcgaca tcagctacta cccacccccc acctgctgcc gggataagtg
    12541 ctataaatgc ggcagcagtt gccacaaaga gtacaggtgc tgcccaaaag attggcttat
    12601 ctgctttggc aggaattatg atttctttag acaatagttt gattccatcg ctcatcagct
    12661 gcaatattcc ttcgacttta ccacagtaga atggccctac tcgcagctgg agtttggcaa
    12721 gcatctttct ttcaacaaaa attgttccag ctgctattag tgcggcaaat ccaaatcctg
    12781 gaaatactgc aattctaaaa aattctgttt ggagtaacga ctttagtaat ggaatctgcc
    12841 ttgacggatc agccaaagtt gtaaatgcaa ggtatggatc cagcagctta ccaccaatta
    12901 ctggcagctc caaatagaac aaaaggatca tcactagtgg tatgccagtt agtgtgagca
    12961 gtattattat ccaaaataga ttatcaagaa tagacttgat aaaataacta aatcggaatt
    13021 ttggtgcaag tgcggacatt tacctgtcag cctccactgg ccagtagttt aggctccagt
    13081 aaactgccgg catgtttcct agtttttctc cttttagcag atacggcagg cagatcaaat
    13141 ttctaaacga gcctacgctc atcttaagac ggtatggctc cggtttgctg cgagatacaa
    13201 tgtagcatcc aagtgcgccg cgtccagatt cgactcgctt gtagacttcg tcgtttccac
    13261 cctttgggtt tggctttagc tttactctga cagagccaga ttttggcatc ttttgcagcg
    13321 cctgtcgtat gatgttgcag ctctcaagca tgtcaagcca tggaatcttg gagcgtgcgt
    13381 acgagtctcc ggcctttagg acgtttgtct taatgtcaag ctcatcatat acatcgtatg
    13441 gctcttttgt tctaacatca aagtccacac cgcttgcacg caaagtagac ccagtcgttc
    13501 caagtctgat tgcgtcattt cttgaaagaa caccgcttcc ttcggttctt gaaattaaaa
    13561 tcgggttgtc gtaaaaaatg tctgcatatt ctttgattcg tttttcaaaa taattgacac
    13621 gcctcagaca catttcctca aagttagctg gaacgtcgtt tcgaattcca cctgggacaa
    13681 aatatgcatg tgtaacacgt gcccccgtca tcttttctaa tgcgtcgatg aggagctccc
    13741 tgtctcctgc aggccacatg aacatagtcg agtggcccaa aaagattcca tatattgcaa
    13801 gccagtactg cgtgtatacg cacctgttaa gctctgctgc aattactcga atgtatttgg
    13861 cgcgctctgg aacttcgagt cctagcagtt cttctacgcc aaggcagtac ggatacagaa
    13921 tgttacatga atcatgaatt actggtcgtt caaggtgtgg gatgtttgtg atatagtttc
    13981 tatgttcggc catcttttct tcgccacgat gtacatatcc tgggtctggg tcgcaggaga
    14041 caatatagtc gccatctact ttgactatca gcctcatgtg gcctgatcct gggtgttgcg
    14101 gtcctacgtt gagcgtcatt atcttttcat ccactttttc tatttgcagg cctggcggta
    14161 gttgtgtagt catcttatct tccctttaac ttaaagtcct tgcgaagtgg tggtaggtct
    14221 gaccagtcct ctggcaaaag aagtcttctg ttatccggat gaccctcaaa gtaaacgcca
    14281 agcatttcaa aacactccct ttcatggaac tctacgctgt agaatatatc tctaagactg
    14341 gcaagttttg tggaatcatt tccaggattt ggatcgtctt cccgtggaac acctgtcgca
    14401 agtgccaaga tttgttttgc tagttccgga tcagtatagg agccaaggtg atacaccacc
    14461 tcgatttcct ttttgtcagg ataatcgacg ccggtcacag attctgcatg gtcaaagtgt
    14521 aattcgtcgc ggatgaattt tgcgacatca agtatgtcct cttttgctac ctttactcgg
    14581 attcttgtcg gcttgacaaa ctcgattgct acttttgatg cgaatttagc ttcaagcttg
    14641 tctgtgattg atttttcaaa ctttggtagt tcttttgtct ctgccttagc tactggtttt
    14701 gtttgttctg gagttgtgct cattaagatt ctctacctag ccttcatcct cttgattttt
    14761 tcctgcagta acatgcatcc ttgtatgagg gtttcaggtc taggggggca tcctgggacg
    14821 tacacatcta ctggaaggac gccatctatt cccggcagga cattgtacga atcaaagtac
    14881 agccctccag tgatggcgca tgctcccatc gcgattacga attttggttc cggcatttgg
    14941 tcatatacga gtctgagtcg cggagccatc tttcttgtga tggttccttg cactactacc
    15001 agatcgcatt gtcgcaaaga tccgaaggct tcgatgattc cgaatctttc cacatcgtat
    15061 cttggaccag atgcagcgcc aaactcgaca ctacaacatg ccgtttctat gtgtactggc
    15121 cacaaagacc acagtctact ccagttaatg gcgtagccaa gtggtgcacc gattgctttt
    15181 acaaggacgt ctcctagctt gcctacaaag acgtttgcgt tatacggagt taagagatcc
    15241 tttagcaatc taatccctac ctgtcctttc gtctgaatgt ataattagtc ttaccaaatg
    15301 tctcgcctct ttgattgata gagtgcaaag gccattgccg caaacattat tcccaaaaac
    15361 gcaattattg gcaaagtcgc agtcttaggc aggctcagaa tcgagcttcc ccaagcatat
    15421 aggaatattg ccataacatc aaaaatgaca aacattagca catacgcata atactgcatc
    15481 ataaagtggg ttcttccctc accctttgga acctgacccg actccattgg aaggaatttg
    15541 actggattgc tatggcgccg tggtgagatc atacgtgaaa tcaatagtgc aggccccact
    15601 gcaactatag caaagccgaa cattagaaga acatgactat agttagctgc atattcccca
    15661 accaaatcag cttgacaccc tattgtttat ataaaaaggt atgcaaaaaa ttgcacaaaa
    15721 aattgcgatc agaaattttt tgcatttgat tgatatacat cgaacccaag tttttcattt
    15781 atggcagtaa atgttggcga caaggctcca gaatttgagc ttgtggacac tgaactgaaa
    15841 atgagaaaac ttggcgactt taaaggcaaa aaggtagttt tgtcattttt tgttgccgca
    15901 agttctcctg tgtgcacaaa tgaaatgtgc acgtttcgtg atagatggag tgagatatca
    15961 aaccttgggg ctcaggtaat tggaattagt aacgacggac catttgcaaa caaggcgttt
    16021 gcagaaaagt tcaatcttaa atttccaata cttggagact acaaaagcaa aacaatacgt
    16081 gactatgaca tcctcatgcc ggatctgttg cacgtcaaag gatacgatgc tgcaaaacgt
    16141 tctgtctttg ttgtggacga agacggtaaa gtagtctaca gatgggtttc cgacaatcca
    16201 ctagttgagc caaactacca agagataatt gattttctaa agaaagaaaa gtaatttttc
    16261 tcttttttat tctatttccg ccaagacatc attttttgca actgatgccc cggattttat
    16321 cttgagtttt ttgataactc catctttgtg tgattttatc gatacttgca tcttcattga
    16381 ttccaagaca caaacgacat cacctttttt gacgctgtct ccttcggtta cgttaactga
    16441 gactaccttg cctggaatct ggctgcgcag actaatttgc gcatctacac tgccaccccc
    16501 acccgtattt ttgtacacaa tttcatcgaa tttagaattc gggtttagtt tcataggaat
    16561 gccatctact actaggttca tttgagccgt agcactttca agatacttgg ctttgtggta
    16621 tttttgatct aaaatgaact caattcccct agaatccatg tgtagaattt ttaactggtg
    16681 ttcttgagtg cctatcttaa ttacatattc attgtttccc agatttttga gtatttgggc
    16741 gttaaatgtt ccttctacat cctctatttt gtattccaca atctcaccct agtttgcttt
    16801 tccatgaatg attgctatta gctgagctaa ttactcggct cttaaaatat tctgaatgca
    16861 ttatagctgc ggcaagtgcg ggttctttct tttcagattt ttcagatttg acatcttctg
    16921 tcaatttctc aatgatgcca tatcttttca aaaagtccgt tgaaagttca ccttttttgt
    16981 attcttccgt ctttagaatt gttttgtaaa gagggatcga ggtctcaaca ccttgaatat
    17041 aaaaatcgtt taatgcgttg agcattcgga gtcttgactc ttcaaaattt tgtccccatg
    17101 tgactagttt tgccatgagc gaatcataga atggtgaaac tgtacatcct ggatacaggt
    17161 aagtgtcaca tcgaactccc ggcccggatg gaattgtaac gtccggtaca ggaccagtag
    17221 atggtgcaaa gtccaaaaac gtatcttctg catttattct gcattcaatt gcatagccat
    17281 tcatctttag atctttttgc ttaaatggaa ttggctcccc atttgcaata tcaatttgta
    17341 attttaccaa atctaatcca gaaacaagtt ctgtaattgg atgctctact tgtaatctag
    17401 cattaatttc aataaaataa aactccccat tatctgccct aagaaattct gcggttccaa
    17461 gattggtata tcctactgcg ttagctgcgc taatgactag ttctccaatt ctatcccttg
    17521 ttttttgatc aactgctggc gagggagttt gctcgataag cttttggtta cgcctttgaa
    17581 tcgagcattc cctttcaaat atgtgaatgg tattgccatg cttgtctcgc gctaactgat
    17641 actcaatgtg acgaattttc tcaaggaatt tttcaacaag gattgcagat ttcccaaagg
    17701 cggctattga ctcacctgtt acagtctcat acgcttcacg cagttccttg tcattgttta
    17761 ctagcctgat ccctcgtcct ccaccaccat aaactgactt tagtaaaact ggatatccga
    17821 ttttgtttgc aatgcttaaa gcctcttcaa cgtcttttac aagatctgga cttcccggca
    17881 ctgtaggaac tttggctttt aacatggcag ctttgcacct catcttatcg ccgcaaagat
    17941 ccatcgatgc agaagacgga ccgatgaaat tgatcttatt gtcttcacac agttttgcaa
    18001 agtcagagtt ttcagaaagg aagccatagc ctggatggac tgcatctgcg ccagacgcaa
    18061 gaatgacttc catgattttt tcttgattta gataactttc ttttggcgct gccttgccaa
    18121 tatgatacgc ttcatttgct tgttttacat gcaaggaatt gtagtcttcg tcagaataaa
    18181 ctgcaacagt tttgattccc aaagctttgc atgtgcgtat gactctaagg gcaatctcgc
    18241 ccctatttga aatcagaatt ttttcaatca taatactcac aagttgatgt ttccgtgttt
    18301 tcttggaagc tgcttgtccc gcttgtttgc aagcatctca agcgccttaa tcagcatcgg
    18361 ccgtgtttgt gccgggtcaa ttactgcatc gactgatcca tttgatgcag ccacgtatgg
    18421 gtttgcaaat ttctcgttaa attcatcagt tagttgtttt ttgagctcct ttggattttt
    18481 tgctgcgtca agatcctttt tgttcataat tttaactgcc gactcagagc caaggactgc
    18541 tatttgtgca gttggccatg cgtagttgat gtcggttcgc aggtatttgc ttcccattgc
    18601 gatatatgcg ccaccgtatg ccttgcctat tacgagtgtt attttgggaa ccgttgcttc
    18661 acaatatgca tacaaaagct tgcttccgtg tctgattatg ccgttgtgtt cttggtttgt
    18721 gcccggcatg tatcctggtg tgtccaccaa agaaattatt gggatgttaa aggagtcgca
    18781 aaatctgatg aatcgtgaag ccttgtttga cgagtcaata tcaagtgcac ctgcaagata
    18841 aagtggctgg tttgcaacaa tccctacagt ttttccatgc attcttgcaa atcccaccat
    18901 aacattttgc gcaaacaatt catggacttc aaagaattgg tgattgtcca caatcgagag
    18961 aactatttct ttcatgtcgt acggttgcag cgagttttct ggaattttgt ttattagatt
    19021 gtgatccatt ctgtttggat catcatcgct tacaaccagt ggggagtctt ctgtattgtt
    19081 tgcaggaagg tatgaaagca aagttttgat cttatcaaaa cagtcatact cattttttgc
    19141 aacaaagtgc gcaaccccgc tttttgttcc gtgtgttatt gctccgccca gatcttcaaa
    19201 cgatacctct tcaccaagaa ctgtctttac cacctcagga cctgtcacgt acatggttcc
    19261 ggctttttca accattataa caaagtccgt cattgcaggt gaatatactg cccctcctgc
    19321 agatgggcct atacttgcag tgatttgtgg aacgacccca gaggcaagct cgttgtgata
    19381 gaaaatgtct gcaaacccat caaggcttag aatgccttcc tgaattcttg ctccgccaga
    19441 gtcaattatt ccaattattg ggcatcctgt gcgtgttgca tgatccatga gttttgtaat
    19501 ttttttagcc cccatctggc ttagcgttcc acccagaact gtaaaatcat atgcgaagac
    19561 aaacacctgt cttccgttaa ttgtgccata accagtaatc acgccgtctg taaagaactt
    19621 ctttttttgc atgtcatatt cataataatg atgagttacc aaggcgtcaa tctcagtaaa
    19681 gcttccctca tccaaaagaa ggttgattcg ttctcttgca gtgagttttc ccttttcgtg
    19741 ctgtgctaga attctgtctt gtccacccgc agtaagtgct ttgttttgtt tttctaaaaa
    19801 tttttcaatc ttttcagaat gcatatgacc ccgcaacaaa tgaccgcata gcagactata
    19861 ttaattttag atccatgatg gagctttagg gtaaaaaact agatcgcgtt ttgggtttgg
    19921 tttttgcgca gcacaaaaaa tttgttagtt agattttgtt aaaagatcat tctgttctta
    19981 atttgtctct gccgcccttt gccgtcctaa agacattcat tccaacatta taattctcat
    20041 acagatagcc aagtgtcttt agatcctctt ctgcttgatt gatttttact ttatttgctt
    20101 ccggatcacg ctttaatcgc tcaagtgttt ctattttttc tttggtcaag tcaacgatac
    20161 ctagctcata gtttgatgtt ctccgaaatt caggactgag aacacgtgtg gggttgtatg
    20221 tggggaccag atacgtcatt tcatccattt tttcttcttt ttttggcttg atcttggtgt
    20281 gttcagtcca actagttcaa ttcttttgtg ggcggacttt aagggtttat ctttagtttg
    20341 agcagatttt tgttagtttt tggcagttca gtcttgttga agttttggaa ttgttattgt
    20401 aaatgtggtt ggattgtttt ttactgaaat ggagccgttg tggtcctcaa caatcttcct
    20461 gcatattgat aatcccaatc ccgttcccgt tctttttgtt gtaaataatg gctcaaagat
    20521 ccttggtaaa acctcatcag gtattcccaa acctgtgtct ttgatttcaa tttttacttc
    20581 atcgttttgc tcaaaaatct ggattatgat tgtaccacca tcttccattg catgaataga
    20641 attttgaatc atatttgtta gcaccctttg cattttgtag ctgtcacata cgacagtaac
    20701 atcacagttt ggaagctcga ctagtacatt atctggcttg tgtatattta gaagcgaatc
    20761 atttaaaatt ttcatgatgt tactgccttc aaattgcatg ggtgagtctt tgagaaagtt
    20821 caaaatgtct tcaagctgat cggtgattcc atttacggct ctttccattc tgcctaggga
    20881 atcaagcatt atcttatctt cctgttttga atagcgtaat ttcaaaaggt ccacctgtac
    20941 tttgattacg tttagtgggt ttcgtatatc gtgtgccatc ctagacgata gctcgcccat
    21001 gattgtgaat ttttcattgt tggaaaactt gaatgctttt ttgtcaagct cgcgttgaag
    21061 taacatttgt ttttctgtca ggaatttttt tgcccgctca agactttttg attgtttttt
    21121 aattaatgtc tgaatgttgt ttaactcttt tctctcttcc tcaattatac tgtcaatggt
    21181 actgaccctg tctctccttg tatcctttga atccacgatt aaaatttgct cagctgtatt
    21241 tttcgttaat ttttgtgatt tattgccatc acgtccaatc tgactttctt gttggttttg
    21301 aagatcttca tctgtgtttt gggtgggtgt ttttgagaaa tatatcattc tcccattatt
    21361 agtagatagg tcatcctctg tattcttttt atgaataaac tttgccaaca catacacgtt
    21421 atattgatgt ctcatatcgt catatctgat caaattgcac gagcttgctt taaaatcgtg
    21481 gtaaatgctg gaacaaaaaa caagtttaat aaaatcagat ctgccagaaa aacaacatgt
    21541 tgtttggagt ttttgcgaca catagcccag agtcatgtcc tctaaacaat aaggatagta
    21601 aaaagatctt tttagggatt gagggcaagc taaaagctaa cctaaaaaaa ttcaacattt
    21661 ccaaaatcgt gggtttttac atgtctgttc ttgaacacca gtggatcatc atcttggatg
    21721 caaaaactgc ccacgacatt gagcagtttt gtatcagtgt tgggatttcg gcaattagca
    21781 cagtcaagat tgtgccaatc aatgactttg cagttacggc aaacaagctt agatctcaaa
    21841 aataatttca acgcaagatc attttgtgat gcatcagatt agaagaaatt gtttgcgcaa
    21901 attgaaatgt cgtgttagtc tgtctttagg tttaacaaca caaagttctt ttttactgca
    21961 ttttgataag cgataaaaga atccttagag tcgctgcatt ttttacagat acataattcc
    22021 gatacagact ctctgtcaca gtcatcaaag aattcacatt tttggcatcc ggctggcccg
    22081 ccgcaaatta tacattttag ctcctttact tctttggatg cgcatttttc gtgtttgact
    22141 ccaatccctt ttgcccaaag acccatctca tttacttcga ttttctggct gcatactatg
    22201 caagtgccgg gaaatttcat tggtattttt cgccagctca tcttagcaga tcaatttcct
    22261 tttggagtat ttcgtcatat tccggactta attccatctc tagttttttg tttttcatgc
    22321 aaaacaaaat gtatggaaga taaaatgtcg caattgtact tttcgagaca tgaaattttt
    22381 ttgcaagcaa ggctccaagt gctttgattg tttttccatc ccatcttaat ctgttcaaaa
    22441 gtggccatga aaggttgtac tgtgaaaact gtactggcag attctcctca tacagcctta
    22501 gcaaaatgtt gtctagatat ctaagtaatc gccattcctg cgttctcatt atttttccat
    22561 acaacatgtc tgcttccgat atgacgccaa gcatctttgc gaggtctttg ctctgcaggt
    22621 tgctagacac aatgcttgaa taaaatgcgt tgattttttc tcttggatcg attcgcagtg
    22681 agtacagcac aacctgcgct tcttcttttg attttgcctt aaagaaagcg tttactgctg
    22741 cgtcgatgtt gagagtttca aacgattttt ctgttggggg atcaaagcca gttacatgtg
    22801 cctgcgccat gtttagaagc gatctgatgt ctccgcgtga gtctatgatt atcttgattg
    22861 tagcgccagg cccaagcgta gctccttctt cttttaggac cttttgtagg tacaatccaa
    22921 gtagtcttgg cggaagcggt tttaggtaaa ttggctttgt tgtctttttg atgtttttca
    22981 tcttgtctga ttgatcagag tttgctgcca aaattattgg aactgttggc tctttgagaa
    23041 tttttatcag tgcctctact ccgccaaaat cggcccgacc atgaatccca tctacctcat
    23101 cgacaaaaat cattggcttg ccaagcaacg ttacgtttcc caaaagcggt gtcaagatct
    23161 cttcaatgtt tgccttgttt ctaacatcgc ttgcattaag cccgattagg tcgtatccaa
    23221 attcctttgc tgcaagctgt gcaagtgttg ttttgccaat ccccggagga ccaactagta
    23281 atactggctt tacgcccttt ttccatttta cgagccagtc aacaaatgat ttacgagcct
    23341 cttcgtttcc aaccatatct attatttttt taggtcggtg tttttcagac cacatcatat
    23401 ctttttcact ttggaatttt tgatgaaaat tcatcccaga gatttgcctt ctctagttgg
    23461 ttgaaccagt attggttgta gtgctcaaca gtcaaaaaca aaaactccaa aaattccgtg
    23521 tgagaaaagt cttttggcaa aagctccagt gccagccttg catcgctcag atagagttca
    23581 ctttttttca tggtaaaaac aaatcccgcc tctacattat ttgtcaaaag tgagtaataa
    23641 agaggccacg aaggtatttt gacaaacttg ttttttatcg agtcctcaat ttcgtttcca
    23701 cacccaacag actctgcagc ccttgccatg ccaagataaa agatctcacc aagcggattt
    23761 tttgccaaag ccatgtttct gccggcagtt cccattactg cgcgatattt tttgtagcat
    23821 tggttcatct gttcttccgt aatttttgtt ggattcgcct tgagtttggt atcggtgtac
    23881 tggccaaggc gggcatccct gaatccttct tcaaattcgc gtaggatttc ttctccgcct
    23941 tttgcaattt ttgcacgcgc aagcctcaaa atatatgggt tcatgagctt gtaatcgtca
    24001 agatattcca agtcttcgtc tttgtctacg attctgtcca tcggctcaga caaatcgatt
    24061 gctattatgt ggccgtcaac taggtcttgg cgcatttgag cattcttgtt tccataaaat
    24121 cccgatgcgg cgtcataaag tgcgttaaat actggcagag tcatctttac aaaattagag
    24181 tttaggattc taagaacccg atcctttagg acgtcacggt tttcaagcct tgactttatg
    24241 tcaggaattt gtgaatcttg cagaattatt tcagtagaac cgacctcttg cgcaaattct
    24301 ttttgtgttg atgaggcgga cgcatctgat tttatttctc ggagtaaatc gtctgtgaaa
    24361 cgatccgcaa attttggaaa ttctgcttct gcttcttctt tgtactgatt gaaaagcttg
    24421 aatccttttg atttaaagag gccttgtttt agtatctgtt tgccgggttt tgttgacatt
    24481 agcgcttctt tgcttacaac agactgatcc ttactactca cagtggcaaa ctctttttct
    24541 tgttatttat gctttcaaag tatccttagc ttaaattacg aaaaattcca gacacgttgt
    24601 gcaaccaatt gatgttctca aagaagcatc gaaactagtc tacaaaaatg taaaagatct
    24661 tgctggaacc aaagcagctg cagaagacca tggtgttgga agaggtggag acatatctcg
    24721 caaaattgac attgtagcag aaaaaactgt tttagattat ctaaaaaaaa taaaatttga
    24781 ttgtattgtt cttggcgaag aatgtggcag ggttgaacta tcaaaaa
//
LOCUS       DASWKH010000037        13612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1188426, whole genome shotgun sequence.
ACCESSION   DASWKH010000037 DASWKH010000000
VERSION     DASWKH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188426"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VGA92_02780"
     CDS             complement(<1..891)
                     /locus_tag="VGA92_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9845369.1"
                     /translation="MKPKLRFANQTLDDLKRKNLYRALHYGKINGPYITIGSKKLVNF
                     CSNDYLGLSATKITTNQLQSSSRLVSGNDTSFKKLEETLAEHKSQGASLVFPTGYMAN
                     IGAISALTGKNDLILSDELNHASIIEACRLSDAKTLVYKHNNVDDLAKKLHSQRRSKK
                     FIITEGIFSMDGDFAALDKIADISEKHDAVLIIDDAHGDFTVGDDGRGSANYFGVEKK
                     IDVYISSLSKGLGSFGGYVASDKPIIDLCINKARSFIYTSALPSFLVDLSLQRIAQNR
                     EKRRKKLGQNVLMLCSGLKKI"
     gene            complement(881..1861)
                     /gene="bioB"
                     /locus_tag="VGA92_02785"
     CDS             complement(881..1861)
                     /gene="bioB"
                     /locus_tag="VGA92_02785"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX9845370.1"
                     /translation="MDSDHQFILDCQKKIFSGIKLTEEELVGLINVSGTELVTLSESA
                     NKITRTFQGNKVDVEQLANIKKNYCSEDCSFCGQSAFFNTGVDSYQLLPADQIVQMAK
                     KAKDEGAESYCLVAAWRESSGSDFEKICEIISQINEQVGISIECSLGFLTLEQAKQLK
                     ALGVHRYNHNLETARSKFAEICTTHTYQDRIDTLHIARRAGLELCTGGIIGLGETREQ
                     RLELISDLAKLEPEEVTINLLVPIPGTPLEIQAPLSFEEILRTFAVTRFAVPKSIIKI
                     SGGREVHLQDAGEELLLSGANGIISSGYLTIGGNEMTRDLEMIKKINLEA"
     gene            2078..2287
                     /locus_tag="VGA92_02790"
     CDS             2078..2287
                     /locus_tag="VGA92_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845371.1"
                     /translation="MKYRITLQKDEDGMYVVKCPSLPGCISQGKTRDEAVNNIKDAIE
                     GYLQSLKKHNEPIPPIDEELVEINV"
     gene            2280..2504
                     /locus_tag="VGA92_02795"
     CDS             2280..2504
                     /locus_tag="VGA92_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9845372.1"
                     /translation="MSKLPVLSATKLIKILSKLGYYTDHQTGSHIILRNSKPPFRRLT
                     IPNHKEIARGTLNSIIDDARLTRDEFLSLL"
     gene            complement(2546..3871)
                     /gene="bioA"
                     /locus_tag="VGA92_02800"
     CDS             complement(2546..3871)
                     /gene="bioA"
                     /locus_tag="VGA92_02800"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX9845373.1"
                     /translation="MQNRSFVWQPYTQMKEWAEFPKIVRGEGMWLIDSNGNKLLDGVA
                     SMWCNVWGHSKKEIISTIQRQSEKIIHSSLFNLTNERAETLAKKLVGISPGMHRAFYS
                     DDGSTAMEVSFKIALQYWQNIGEKNKTQFVTLQNGYHGDTFGAMSVGYVPGFFSRFKN
                     QLYKTTQIPFSNSYRIPHDMKINDYYDQVLEKIEKTFSTDDKVAAFVMESGAQMAGGV
                     NIYSAKLQMRISNLCKKYGVLLVLDEIATGFGRLGSFVEYVTQKSRPDIVSYGKMLTG
                     GYITLASTLVSKKIYDAFLGDYSEMSHFFHGHTFTGNPLACAVANENLNLYEKSHLIK
                     KIQKTSHVFEKRLDEISKIGLVGDVRFKGMLMGIELVKSKKSKMPVSFGKSTNKVIFD
                     EAKKHKIYLRSLGNIVMLVPPLAISEDELNLLIDGTISTIKNVSKQINS"
     gene            3947..4633
                     /gene="bioD"
                     /locus_tag="VGA92_02805"
     CDS             3947..4633
                     /gene="bioD"
                     /locus_tag="VGA92_02805"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX9845374.1"
                     /translation="MESYFITATDTGVGKTTITAAMAFALKRLGVNVGVMKPFATGTQ
                     QKTGYKSEDVAILANSAQTTDPEELMNPYFFPVPVSPYAAANKLNVDIDVDMVMDRFE
                     KLQAMHDVMLVEGIGGILTPILKNYCIANLIKDMNLDTLVVTSSRIGTVNHTLMTLNV
                     AQQYGLKIRGLIINNIDPAGYDVNELKNDLKNLSGVDVLCTIPHLNNAKMGEVAEILQ
                     RSNLISQLTS"
     gene            complement(4628..5182)
                     /locus_tag="VGA92_02810"
     CDS             complement(4628..5182)
                     /locus_tag="VGA92_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845375.1"
                     /translation="MENTPIKDILYGEINHITESRILQEISSKNYSKIINEILTRCIP
                     IISKLDGDSFKIFGTFAESMMHYLLTNALIPSQRKITIKNIDLDITIPDSRTLDTSPK
                     DAIILVFAKTSEQDTITNYLKKLQKIQPIKENIWIISKSKLDIPCKTYELESKERLST
                     ILDDINDFLLSRPQSKFRIFKTKL"
     gene            complement(5219..6613)
                     /locus_tag="VGA92_02815"
     CDS             complement(5219..6613)
                     /locus_tag="VGA92_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX9845376.1"
                     /translation="MAAGIDDIAVYIPRLFLDSADFAVARGLDPAKLKRGLGISKMAI
                     VDANQDPACLAANACLKVMQRNNIAPKDVGRLYVATESALDESKALNSYVIGMLEQVY
                     GPDSFEHCGGIECKFACVSGSYALYDNCNWIRAGEAEGKSAIVVVSDIAKYDMGSAGE
                     YTQGAGAIAMLLNDNPRLLQFDTKVTATSIKNEYDFYRPFGKETPIVHGQYSNLLYMI
                     QVKKAFEIYKKKALDTGIIKIKEGETILDHIDYLCMHLPYSNMGKKALAYLLRHEWRH
                     LPRWKNIIQQTVMEEPTPKDKFGTIESILADGEYMGKDHEFTKLFTKTKEYQQFYEDK
                     LASSLIASAMIGNLYTASLYLGFRSCLEFEFQKGVALEGKHFGFGSYGSGSSAMIFSG
                     IVQPSYKELVKNMNLEVEIGERKKLSLKEYEEIHENKLGPHQNLLDAKEEFVLISVEN
                     TPESRGERKYAFCG"
     gene            complement(6771..7334)
                     /locus_tag="VGA92_02820"
     CDS             complement(6771..7334)
                     /locus_tag="VGA92_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX9845377.1"
                     /translation="MEFNCVKDCSQCCVERQYYPTKKFGKVGVLIIPEEKERIEALAE
                     RHHLPITILPRIGVSHQNHDGPDQIMAYQMMGKEDNGNTCPFLDTESDKRSPHGGFVC
                     KIYNERPLACKAYPLIESKPIILDSKCKFCQHNQTADSNLDGEVEALIKIQSKMSSDA
                     PIIWRYATGIGDVEDRSQIQKGWIRES"
     gene            complement(7363..8079)
                     /locus_tag="VGA92_02825"
     CDS             complement(7363..8079)
                     /locus_tag="VGA92_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238549798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9845378.1"
                     /translation="MIHAPSLGIGAAIAAVAIIGIFYAMDNAPTDTAFKTEDIKPSEV
                     ENQAGDAVKLSIFSENASPYLGDASAPITIVEFGDYQCFYCNKFFHETEDQIYENYIK
                     TGKAKMIFKDYTIIGPDSIVAAHAAHCADEQGKFWEYHDTLYNNWNGENNGWASAENQ
                     IKFAQDLGLDMTAFSECMSSEKYKQKIQASTNDAQTLGLSGTPGFFIIGPNNNVVRVP
                     GAQPFDVFVNVLESEELKGN"
     gene            8176..8379
                     /locus_tag="VGA92_02830"
     CDS             8176..8379
                     /locus_tag="VGA92_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845379.1"
                     /translation="MSNDKLWIITVTIGFSLLLTLSYFVLVPMASEQGMLVRTDAGLN
                     NAIEKFEQLKEQKQGSDESDLNT"
     gene            complement(8439..8621)
                     /locus_tag="VGA92_02835"
     CDS             complement(8439..8621)
                     /locus_tag="VGA92_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845380.1"
                     /translation="MSGLGKMEEGRAKDMGLYQSPRKAETGLGSDRLPQELREFTPAS
                     QDSTSKKWARDQKLGR"
     gene            8764..9813
                     /locus_tag="VGA92_02840"
     CDS             8764..9813
                     /locus_tag="VGA92_02840"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX9845381.1"
                     /translation="MKKVFVNGYGSIGSRVAQFIKDDPEIKVLGVGKYSPDDKVNDAI
                     SRGFDVYVPTKRIDSFKNYKIKGVIEDVLDDCDIVIDASPGGMGYTNKKQLYEPRGIM
                     AIYQGGESVFGDERVSDLLFNSRVNYTEAFEKKHVMQGSCNVTGMGRVLQPLREKYGS
                     QIVRFDAVLVRRWADLEQTDKTVPDTIEWTSQPHHQEDVKHYMGKDMPLFIQAIKVPT
                     RQMHVHLMNIRFKDVAPKPSEILEIFKDEHGVATLWTAKGTKQIRDAAESMKFSFKDT
                     NMIHIHANMLESVGDTVKMVYSDDQTGIVIPENHILMQAMLFQKPYSEAFAHTEGLFH
                     MNEKKRNLEEFFTKK"
     gene            complement(9831..10772)
                     /locus_tag="VGA92_02845"
     CDS             complement(9831..10772)
                     /locus_tag="VGA92_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9845382.1"
                     /translation="MGIGSKRAVYAALFGNLGIAITKFIAAIFSGSASMWAEAYHSSS
                     DTFNQILLLFGMKTSTKAASYRHQFGYGREMFFWSFIVATMIFGISGILSLEQGVASL
                     LHGPRIIENVLLSYVVLVIAFGFEANALRIALKQFTKEIRENGEKITFSSMINHFKES
                     KDTALLTVVVEDTAALLGIVVAAIGIFLSDITGNSVYDAISSLFIGGILMFFAFFLAK
                     ENRGLLIGESITPREYRRIVKLIKEMPEVNRIVSMRTMHLGPEDILVGVQVNLVNNLD
                     TDKIETLTDAIEQKIMQVIPNTNREHIFVELERERIR"
     gene            complement(10823..11728)
                     /locus_tag="VGA92_02850"
     CDS             complement(10823..11728)
                     /locus_tag="VGA92_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9845383.1"
                     /translation="MSHTNKGTRADSATILRKGQLAAKKSVITLAAIGIVEITVAVFT
                     GSITLTADGFDSIADAMISFIVWFGIMFAKKSRSNTFQFGYRKIEVLAAFIAAIIIII
                     LGIFIVIHAIDALDKPKTFQYPEIVIITLIGAGSVSLHRAFVIRKIAIESDLVSLKLD
                     AKNSIKDGTASFVGVASVVAATYFGFTFMDAIGGIIIAGYIFIVAYTAIKESALVLVD
                     AVDNPLLSDSIKLTIEKKFNIKTNEVFLRPIGNDFNAEIHIMLPKETRLDETSRIVNE
                     VSRVIQDDFALARVVIVPLPDNVKS"
     gene            complement(11790..13127)
                     /gene="thrC"
                     /locus_tag="VGA92_02855"
     CDS             complement(11790..13127)
                     /gene="thrC"
                     /locus_tag="VGA92_02855"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9845384.1"
                     /translation="MKDNAFLKCIDPSCGLEYPITSGNIECDRGHMLDVKYKNIPSQE
                     LKELFYTRRNPQGSIFNESGVWRFRELINFCQIDTTSIQECAKFLVSLDGAEGRQSKP
                     YQMSKVANFIGVNHDNLWLQPEGYNPSGSFKDNGMSTAITHAKLIGAKKIVCASTGNT
                     SAAAAMFAANENMECDVYIPAGQIAPGKLSQAYQFGAQIIQVQGNFDDALTKSLQDAK
                     KYEGYTVNSINPFRIEGQKTIPYRAIEYLNWEAPDWIVYPGGALGNTSSCGKAIMELY
                     EWGWIKKIPRIAVINAEGASTLYDLYNGNFEGAELRWNKGKPDTEMINRYYKDLDQKG
                     IRPKTKATAIQIGRPANILKGLRALEYTNGVVEKVSDSEMLDGMSIVGLNGFDCEMAS
                     GATPSGIKKLVEKEIIKKDDVVVGILTGRQKDSSLPVDYHNNPANKFAIPPKG"
     gene            complement(13148..>13612)
                     /locus_tag="VGA92_02860"
     CDS             complement(13148..>13612)
                     /locus_tag="VGA92_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9845385.1"
                     /translation="YQIIHERIKNRRKDFLHQLSTQYAKKYNVVFVERLQKLNMVKNH
                     KLARNILDSGWGTFTNMLDYKTMLAGVTAKNTTIDCSRCGNTVPKSLAVRIHRCNVCG
                     LMLDRDHNAAINILKNGLDIFNIKLPQELREVTPVEITKWSMKQEESIGQVR"
BASE COUNT         3677 a         2840 c         2655 g         4440 t
ORIGIN      
        1 aatttttttt agtccagaac aaagcatcaa tacgttttgc ccaagttttt tccttctttt
       61 ttcccggttt tgggcaattc tttgcaatga caaatctaca agaaaagagg gaagtgcgga
      121 tgtgtaaata aaagatctgg ccttgtttat acataaatca ataattggtt tgtcagaagc
      181 tacataaccg ccaaaggagc caagcccttt gcttaggctg cttatgtaaa cgtctatttt
      241 cttttcgact ccaaaataat ttgcagaacc ccttccgtca tcgcctacgg taaagtcccc
      301 atgggcatca tctattatta atacggcatc atgtttttca gaaatatctg caatcttatc
      361 tagtgcagca aaatccccat ccatgctaaa aattccttca gtaatgatga atttttttga
      421 tcttctttga ctatgaagct tttttgccaa atcgtccaca ttgttgtgtt tgtagactag
      481 tgtttttgcg tcagacaacc tacatgcttc aatgatgctt gcgtgattca attcatcact
      541 tagaattaga tcgttttttc cagtcaaggc ggaaattgca ccaatgtttg ccatgtatcc
      601 agtcggaaaa accaaagatg cgccttgaga tttgtgctct gcaagggttt cttctaactt
      661 cttaaatgat gtatcattac ctgaaacgag tcttgagctt gattgaagtt gattagttgt
      721 gatttttgtt gcagataggc ccaaatagtc atttgagcaa aaatttacga gtttttttga
      781 gccaattgtg atgtatggcc cgtttatctt accataatgt agtgcgcggt acagattttt
      841 tctttttaga tcgtccagag tctgatttgc gaatcgcagc ttaggcttca agattaattt
      901 ttttgatcat ttcgaggtct cgcgtcatct catttccacc aattgttaga tagcctgagc
      961 tgatgattcc atttgcaccg cttaagagta gttcttcgcc tgcgtcctgc agatgtacct
     1021 cgcgcccgcc tgaaattttg atgattgatt ttggtactgc aaatctagtg acggcaaaag
     1081 ttcgcaaaat ctcctcgaag gaaagcggtg cttgaatttc aagtggtgtt ccgggaattg
     1141 gaacaagaag atttatcgtt acttcctctg gttcaagttt tgccaaatct gaaattagtt
     1201 ccagtctttg ttctcttgtt tcacccaatc cgattattcc gccagtacaa agctcaagtc
     1261 ctgcccttcg tgcaatatgt agcgtgtcta ttctgtcttg gtatgtgtgg gtagtgcaaa
     1321 tctctgcaaa cttggacctt gctgtttcca agttgtggtt gtatctgtga accccaagtg
     1381 ccttgagttg ttttgcttgc tctaatgtaa gaaacccaag gctgcattct atgcttattc
     1441 caacttgttc gtttatctgg ctgatgattt cacaaatctt ttcaaaatca gaaccagatg
     1501 actctctcca tgcagccaca agacagtatg attctgcgcc ttcatctttt gctttttttg
     1561 ccatctgcac tatctggtct gcaggcaata attggtagct gtcaacgcca gtgttaaaaa
     1621 acgctgactg gccacagaaa ctacagtctt cactacaata attcttttta atatttgcaa
     1681 gctgttctac atccactttg tttccttgaa aagtgcgtgt aattttattt gccgattctg
     1741 aaagtgttac aagttctgta cctgagacat tgatcaatcc tacaagttct tcttcagtta
     1801 gcttgattcc agaaaagatc tttttctgac aatctaaaat gaattgatga tcagaatcca
     1861 atgtttcgta acaagttttt acggttaatt aagattttca aattgttaac catgattgct
     1921 acatgtggtt aacaatccga tagttattga ggaaattcat ctagttgtga accgaatgag
     1981 ttgcaggtac aaattcactt ggatcacagt gattttgttg actgtttatt catcagactt
     2041 gaatggctta aataagaaaa cttccattct aagttagatg aaatatagaa ttacactaca
     2101 gaaagacgaa gatggcatgt atgttgtaaa atgcccatca ttgccaggct gcatttcaca
     2161 gggaaaaaca cgagacgagg cagtaaacaa catcaaagat gcaatagaag gatatcttca
     2221 aagtctaaaa aaacacaacg aaccaattcc gcccatagat gaggaattgg tagaaatcaa
     2281 tgtctaaact tccagtttta tcagcaacaa aactaatcaa aatattatca aaattaggat
     2341 attatacgga tcatcaaact ggaagccaca taatactgag aaatagtaaa ccaccttttc
     2401 gaagactaac aataccaaat cacaaagaaa tcgcacgggg taccttgaat tcaataatcg
     2461 atgatgccag gctcacaaga gacgagtttt tgtctttatt atagaaacca aaattagtag
     2521 accaatgggg ttcgtgacct aatttttaag aatttatctg ctttgataca tttttgattg
     2581 tgctgatcgt cccatcaata agcaagttca gctcgtcttc tgaaattgca agaggtggta
     2641 ctagcatcac aatgtttcca agcgagcgaa ggtagatttt gtgttttttt gcctcatcaa
     2701 agattacctt gtttgtagat ttcccaaaag aaacaggcat cttgcttttt ttgctcttta
     2761 ccaactctat tcccatgagc attcctttga accgtacatc gccaacaagc ccaatcttac
     2821 tgatttcgtc cagtcttttc tcaaagacat gagatgtttt ttggattttt ttgattaggt
     2881 gtgacttttc atatagattc aagttttcat ttgctactgc gcaggcaaga ggatttccag
     2941 taaacgtgtg gccgtgaaaa aaatgactca tttcagagta atctcccaag aatgcatcat
     3001 agatcttctt tgaaactagc gttgaagcca aagttatgta gccaccagtc agcattttcc
     3061 catatgagac gatgtctggc ctgctttttt gcgtcacata ttcaacaaat gagcctagcc
     3121 taccaaaccc tgttgctatt tcgtctaaaa ccagcaagac tccatatttt ttgcaaagat
     3181 tgctaatcct catttggagc ttagcagaat agatgttgac tccgcctgcc atctgggccc
     3241 cgctttccat cacaaaggcg gcaaccttat cgtcagttga aaatgttttt tctatttttt
     3301 ctaaaacttg gtcataataa tcattgattt tcatgtcatg tggtattctg tagctgtttg
     3361 aaaatggaat ctgagttgtt ttgtacaatt ggtttttgaa cctagagaaa aaaccgggaa
     3421 catacccaac cgacatggca ccaaacgtgt ctccatgata cccattttga agcgtaacaa
     3481 attgtgtctt gtttttctca cctatatttt gccaatattg caatgcaatt ttaaatgaaa
     3541 cctccattgc tgtggaaccg tcatcagaat agaaagctcg atgcatgccg ggtgaaatcc
     3601 cgacaagttt tttggcaaga gtttctgccc gctcgtttgt caaattaaaa agtgacgaat
     3661 gtattatttt ttctgactgt ctttgtattg tagatattat ctcttttttt gaatggcccc
     3721 agacattgca ccacatgctt gcgaccccat caagcagttt gtttccatta gagtcaatga
     3781 gccacatgcc ttctcctctt acaatttttg gaaactcggc ccattccttc atctgggtgt
     3841 atggctgcca aacaaagcta cggttttgca ttgtctgggt tttccaaact gattttaata
     3901 atcaaccgct gaaagattaa tcaagtgatt tggctgaatt gttgcattgg agtcctactt
     3961 tataaccgca acagatactg gcgttggaaa gacgactata actgcggcga tggcttttgc
     4021 actaaaacgt ctgggggtga atgtgggggt aatgaagcca tttgccacag gcacgcaaca
     4081 aaaaacaggc tataaatcag aagatgttgc aattttagca aattctgccc aaacaacgga
     4141 tcctgaggag ttgatgaacc catatttttt tccagttccc gtctctccat atgctgctgc
     4201 aaacaagctc aatgtggata tcgacgttga catggtcatg gacagattcg aaaaactgca
     4261 agccatgcac gatgttatgc tagttgaagg aatcggtgga atcctaacac caattttgaa
     4321 gaattactgc attgcaaatc tcataaaaga catgaacctt gacactctgg ttgttacaag
     4381 ctcaaggatt ggaaccgtaa accacacctt gatgactctc aatgttgctc agcaatacgg
     4441 cttgaagata cgaggcttaa tcataaacaa cattgatccg gctggctatg acgtcaatga
     4501 gctaaaaaat gatctgaaga atctgagtgg agttgatgtt ctctgcacga tccctcattt
     4561 gaataatgcc aaaatgggcg aagttgcaga aattctacag cgcagcaatc tcatttcgca
     4621 actaacctca tagttttgtc ttaaaaattc taaattttga ttgtggtctt gataaaagaa
     4681 aatcattaat gtcgtcaagt attgttgaaa gtctttcctt gctttcaagc tcatatgttt
     4741 tgcaggggat atcaagtttg gatttggata ttatccaaat gttttctttt attggttgaa
     4801 ttttttgcaa ttttttaaga taatttgtga tagtgtcttg ctctgatgtt tttgcaaaaa
     4861 ccagtattat tgcgtctttt ggcgatgtat cgagtgttct tgaatctgga attgtgatgt
     4921 ctaggtctat gtttttgatc gtaatttttc gctggcttgg aattaacgcg ttagtcaaaa
     4981 gataatgcat catgctttct gcaaatgttc caaaaatctt gaaactatct ccatctaatt
     5041 ttgaaatgat tggaatgcat ctggtgagaa tctcgttgat tatcttgcta tagttcttgc
     5101 ttgaaatttc ttgcagaatc cttgattctg ttatgtgatt tatttcgcca tacaatatgt
     5161 ccttaattgg agtgttttcc acgactaaac ccacaaaaac tgaatttctt gccttggctt
     5221 acccacaaaa ggcgtacttg cgctcgcccc tgctttctgg ggtgttttca acgctgatta
     5281 gaacgaattc ttctttggca tcaaggaggt tttggtgtgg gccaagtttg ttttcatgta
     5341 tttcctcata ttctttgagt gaaagttttt tgcgctcgcc aatttccact tcaaggttca
     5401 tgttcttgac taactccttg tagcttggtt ggactatacc gctaaagatc atagcgctac
     5461 ttccactgcc atatgatcca aatccaaagt gttttccttc aagggctact cctttctgga
     5521 attcaaactc aagacaactt ctaaatccca agtacagtga tgcggtatac aggttgccaa
     5581 tcatcgcaga tgcaatgagt gaacttgcaa gtttgtcttc gtagaattgc tggtattctt
     5641 ttgttttggt aaacaatttt gtaaattcat ggtcctttcc catgtattcg ccatctgcta
     5701 agatggattc tattgttccg aatttgtcct ttggtgtcgg ctcttccatt actgtctgct
     5761 gaattatatt cttccatctt gggaggtgtc tccactcatg tcgcagaaga tatgcaagcg
     5821 ccttttttcc catgttgctg tatggaaggt gcatgcaaag atagtcgata tgatctaaga
     5881 ttgtttctcc ttcctttatc ttaattatgc cagtatcgag tgccttcttt ttgtaaatct
     5941 caaaggcctt tttgacttgg atcatataca acaagttgga atactggccg tgaacaattg
     6001 gtgtctcctt gccaaagggc ctgtagaaat catactcgtt tttaattgac gtagcagtaa
     6061 ccttggtgtc aaactgcaac agtcttgggt tatcatttag aagcatcgcg attgctcctg
     6121 cgccctgcgt gtattctcca gccgaaccca tatcgtattt ggcaatatcc gacactacga
     6181 ctattgcaga cttgccttct gcttctcctg ccctaatcca gttacaatta tcataaagcg
     6241 cataagaacc agaaacgcac gcaaatttgc actcaattcc accacagtgc tcaaacgagt
     6301 ctggtccata gacttgctca agcatgccaa taacatacga gtttagcgcc tttgactcgt
     6361 caagggcgga ttcagtggcg acataaagcc ttcctacatc ttttggtgca atgttgtttc
     6421 tctgcattac tttgaggcag gcatttgcag ccaggcatgc tgggtcttgg ttggcatcca
     6481 ctattgccat cttggatatg cccagtccac gctttagctt ggcaggatct aggcccctgg
     6541 ctacggcaaa gtcagccgag tctaaaaata gccttggtat gtaaacggca atatcgtcta
     6601 tgcctgctgc catgagcttt cccttctact tttagatata agggttattg ggtgactaaa
     6661 ttccactaat atgtgatttt aatcaaattt ctcatagttt acgtaaattc gaattattaa
     6721 atcgggtttt tgtacaagaa ttaatcgctt tttgagtttt tgatatagtt ctagctttcc
     6781 ctgatccagc ctttttgaat ttgtgatctg tcctctacat cgccaatgcc tgtggcatac
     6841 cgccaaatga ttggtgcatc agaagacatc tttgattgga tttttatcaa ggcctctact
     6901 tcgccgtcga gattactatc tgcagtttgg ttgtgctggc agaacttgca tttagaatca
     6961 agaattattg gctttgactc tattagcgga taggccttgc acgcaagtgg cctttcattg
     7021 tatattttac aaacaaatcc tccatgtggg gatcttttgt ccgattctgt gtccaaaaat
     7081 gggcatgtat taccattgtc ttcttttccc atcatctggt atgccattat ctggtctggc
     7141 ccatcatgat tttgatgtga cacaccaatc cttggaagga ttgttattgg gagatggtgt
     7201 ctttcagcta aggcttcgat tctttctttt tcctctggaa taattaaaac cccaactttg
     7261 ccaaattttt ttgtagggta atactggcgt tcaacgcagc actgcgaaca atcctttacg
     7321 caattaaatt ccatgttatc tattgatggt tgaaggataa aactagtttc ctttgagctc
     7381 ttctgattct aacacgttaa caaagacatc gaatggctgc gcccctggaa ctctcaccac
     7441 attgttgttt ggaccaatta taaaaaagcc aggcgttcct gaaagcccga gtgtctgcgc
     7501 gtcgttcgta ctggcttgga ttttttgctt gtatttctca cttgacatgc attctgaaaa
     7561 tgcagtcata tctaatccta aatcttgagc aaatttaatc tggttttctg ctgatgccca
     7621 cccattgttt tccccattcc agttattgta caaagtatcg tggtactccc agaactttcc
     7681 ctgctcgtct gcgcaatgtg ctgcgtgtgc agcaactatg gaatctgggc ctattattgt
     7741 atagtccttg aagatcattt tggccttgcc agtctttatg taattttcat aaatttgatc
     7801 ttctgtttca tggaaaaact tgttgcagta aaagcactga tagtcgccaa actctactat
     7861 tgtgattggg gcgctggcat ctcctaggta tggggatgcg ttctcagaaa agattgataa
     7921 cttgactgcg tcccctgcct ggttttcgac ttctgatggt ttgatgtctt ctgtcttaaa
     7981 ggcagtatct gtgggggcat tatccatagc gtagaatatg ccaattattg caactgctgc
     8041 tattgcagca ccaattccaa gtgaaggtgc gtgtatcata cggctcctaa aattcagatt
     8101 aatttagcct tttagattgt gtgattgatt cgaacacaat caagctaatc taaactcgca
     8161 aagaacaaga aagcattgtc aaacgataag ctctggataa ttactgtcac cattggtttt
     8221 tcactgcttt tgactctgtc atattttgtt ttggtgccaa tggcaagtga gcagggcatg
     8281 cttgttcgca cagacgctgg ccttaataat gccatagaaa aatttgagca gctcaaagag
     8341 caaaaacaag gctcagacga atccgatctg aacacgtaat tttagcggtg taatctaagt
     8401 gaaccactat cggacaagtc cggtagcctt ttcctgcttc atcgaccgag cttttgatct
     8461 ctagcccatt tttttgatgt gctgtcttgc gacgcaggcg taaattcccg cagttcctgc
     8521 ggtaatctgt ctgaacccaa tccagtttcg gctttacgtg gtgactggta gaggcccata
     8581 tctttagcac gaccttcttc cattttgcct agccctgaca ttggatattt ttgtgaatta
     8641 ttacgtttaa catgctggct attatgtaat taccagctat tcattatcgg cgattcatcc
     8701 accggttaaa accggtggtc ttctcgccga attctctata aatcccgtag aatgataaga
     8761 tacatgaaga aagtttttgt aaatggctac ggctcaattg gaagccgagt tgcccagttt
     8821 atcaaagacg accctgaaat caaagtactt ggcgttggga aatactcgcc agacgataag
     8881 gtaaatgatg ccatatcaag aggctttgat gtttacgtac ctacaaaacg cattgactct
     8941 tttaaaaatt acaaaatcaa aggcgtcata gaggatgtgc tagatgactg tgatattgta
     9001 attgacgcgt cgcctggcgg gatgggatac actaacaaaa aacaactcta cgagccaagg
     9061 gggataatgg caatctatca gggaggtgaa tcagtctttg gagacgagcg tgtttcggac
     9121 ttgttgttta actctcgtgt aaattacaca gaggcatttg aaaaaaaaca tgtcatgcag
     9181 ggaagctgca acgtaactgg aatgggacga gtcttgcagc cactgcgaga aaaatatggc
     9241 tcacaaattg tccgatttga cgcagtactt gttaggcgat gggcagactt ggagcaaacg
     9301 gacaaaacag tgccagacac aatagagtgg acttcgcagc cccaccacca agaagacgta
     9361 aaacactaca tgggaaagga tatgccactc tttattcaag caataaaggt tccaacaaga
     9421 caaatgcacg tgcatctgat gaacattcga ttcaaagatg ttgcgccaaa accatctgaa
     9481 atactggaga tattcaaaga cgagcatggc gttgccaccc tgtggactgc caagggaaca
     9541 aagcagatac gcgatgcagc agagtcaatg aagttcagct tcaaggacac aaacatgatt
     9601 cacatccacg caaacatgct cgagtcagtt ggtgatactg tcaagatggt gtattctgat
     9661 gatcagacgg gcattgtgat tccggaaaac cacatattga tgcaggcaat gctttttcaa
     9721 aaaccatatt cagaggcatt tgcgcacacc gaaggcctct ttcatatgaa tgaaaagaaa
     9781 agaaatttgg aagagttttt taccaaaaag taatttttca aagcgacaaa ttacctgatt
     9841 cgttctcgtt caagttcaac aaaaatgtgc tctcggtttg tattggggat gacttgcatt
     9901 attttttgct caattgcatc agttagtgtc tcgattttgt ccgtatcgag gttgttaact
     9961 aggtttacct ggactccaac taaaatgtcc tctggtccta ggtgcatcgt cctcattgat
    10021 acaattctgt ttacttcggg catttctttg attagtttta ctatcctgcg gtattctctt
    10081 ggcgtgatgg actctccaat tagtaagcct cggttttctt ttgccaagaa aaaggcaaag
    10141 aacatcaaga ttcctccaat gaaaagcgag ctgattgcgt cgtaaaccga atttccagta
    10201 atatctgaca aaaatattcc aatggcagct acgactatgc ccaaaagagc agcagtgtcc
    10261 tctacaacta cagtcagtaa cgcagtgtcc ttgctttctt taaagtgatt aatcatagaa
    10321 gaaaaagtaa ttttttctcc attttctctg atctcttttg taaattgttt tagtgcaatt
    10381 cttagcgcgt ttgcctcaaa accaaaagca attaccaaaa caacatagct gagcagcaca
    10441 ttttctatta tgcgtggacc atgaagcaga gatgcgactc cttgctccaa tgacaaaatt
    10501 ccagagatgc caaaaatcat cgtagccaca ataaatgacc aaaaaaacat ctcgcgacca
    10561 tagccgaact ggtgtcggta gcttgcagcc tttgtactag ttttcatgcc aaacagcaaa
    10621 agaatctgat taaatgtgtc tgaagatgag tggtatgctt ctgcccacat tgaggcacta
    10681 ccgctaaata tcgcagcaat gaactttgta attgctatgc ctaaatttcc gaacaaggca
    10741 gcatagactg cccttttaga acctattccc aattactcaa ttgttaaact ttggacatct
    10801 aataaatcaa tgtcaaaaac ctttaactct tgacattgtc gggtagtggt actatcacta
    10861 cacgtgcaag cgcaaaatca tcttggatta cacgtgatac ttcattgacg atcctacttg
    10921 tctcatcaag ccgtgtttcc tttggaagca taatgtgtat ttcagcgtta aagtcatttc
    10981 ctatgggccg taggaatacc tcgtttgttt ttatgttgaa ttttttttct attgtcagtt
    11041 ttatgctatc tgaaagtagt ggattgtcca cagcatctac caacactagt gcagattctt
    11101 ttatggcagt atatgcaact atgaaaatgt acccagctat gatgatccct cctatcgcat
    11161 ccatgaatgt gaagccaaaa taagtggcgg ctaccacact tgcaactcct acaaacgatg
    11221 cagttccgtc cttaatggag ttctttgcat ccaatttcaa tgatactaga tctgattcta
    11281 ttgcgatttt tcgtatgaca aaggcacgat gtagtgagac gctgcctgct ccaatcagcg
    11341 ttatgatgac tatttctgga tattggaatg ttttgggctt atcaagtgca tcaatggcat
    11401 gaatgactat gaatatgccc aaaatgatga tgattattgc agcaataaac gccgcaagaa
    11461 cctcaatttt tctgtaacca aactggaagg tgttgcttct tgatttcttt gcaaacatta
    11521 ttccaaacca aacgatgaaa gatatcatgg catctgctat ggaatcaaaa ccatcggctg
    11581 ttagtgttat actgccagta aaaacagcta ctgttatctc cactatgcct atggctgcca
    11641 aagtgattac agatttttta gctgctaact gtccttttct taaaattgtt gcactatcag
    11701 ctctagtgcc tttatttgta tgactcaatc tgttttttat ctgataaaat gtagttgttg
    11761 ttgattttta aatcatttgt acgtctgtgt tatccttttg gagggatggc aaatttgttt
    11821 gctggattgt tgtggtagtc aacaggcagg ctagagtctt tttgtctgcc cgtcaaaatt
    11881 cctactacga catcgtcttt tttgataatc tctttttcaa ctagtttttt tattccagat
    11941 ggtgttgcgc ctgatgccat ctcacaatca aagccgttga gtccaactat ggacatgcca
    12001 tctagcatct cagagtctga gaccttttct accacgccat tcgtgtattc taacgctctt
    12061 agtcctttga gaatgtttgc cggcctgcca atttgaattg cagttgcttt tgtcttgggt
    12121 ctaattcctt tctgatccaa atctttgtaa taacggttaa tcatctcggt atctggtttt
    12181 cctttgttcc agcggagctc tgctccttca aaattaccat tatacaggtc atatagcgtg
    12241 cttgcacctt ctgcgttgat gactgctatt cttggaattt ttttgatcca cccccattca
    12301 tacaactcca ttattgcttt accacagctt gaggtgtttc caagcgcacc tcctggatac
    12361 acaatccagt ctggggcctc ccaattgaga tattcaattg ccctgtatgg aattgttttt
    12421 tgaccttcga ttctaaatgg gtttatcgag tttacagtat atccttcgta tttttttgca
    12481 tcctgcaatg atttagtcag agcatcatca aaatttcctt gtacttgaat aatctgagcg
    12541 ccaaactggt atgcctggct aagcttgcct ggggcaatct gccctgcagg aatgtatacg
    12601 tcgcactcca tgttttcatt tgctgcaaac atggcagctg cagcagatgt gtttccagtt
    12661 gatgcgcaaa caattttttt tgcgccaatt aattttgcat gagtaatggc agtagacatt
    12721 ccattgtcct taaatgaacc actcgggttg tagccttcag gctggagcca caaattatcg
    12781 tggttgactc caatgaaatt tgcaaccttt gacatctgat atggctttga ctgtcttcct
    12841 tccgctccat ctaatgacac tagaaacttg gcgcattctt gtatgcttgt tgtatctatc
    12901 tggcagaaat tgataagctc gcgaaatctc caaaccccgc tttcattaaa gatgctaccc
    12961 tgcgggtttc gtcgagtata aaataactcc tttagttctt gtgatggaat gtttttgtat
    13021 ttgacgtcaa gcatgtggcc tctgtcgcac tcgatatttc ctgaggtgat tgggtattcc
    13081 aaaccgcacg acgggtctat gcacttgaga aacgcgttgt ctttcaatct agattgccag
    13141 tgaacttcta tcggacttgt ccgatagact cttcctgctt cattgaccac tttgtgatct
    13201 ctacaggcgt tacttcccgc agttcctgcg gtagtttaat gttgaaaata tctaatccat
    13261 ttttcaaaat attgattgcc gcattatgat ctctgtcgag catcagacca cacacattgc
    13321 atctgtgaat tcgaacagct agggattttg gcaccgtatt gccacacctt gaacagtcaa
    13381 tggttgtgtt ttttgccgta actccggcga gcattgtttt atagtccaac atatttgtaa
    13441 aggttcccca tccagaatcc aagatatttc tagccagttt gtggtttttt accatattca
    13501 gtttttgcag cctttccaca aatacaacat tgtatttttt tgcatattgt gttgacagtt
    13561 gatgcagaaa atccttacgt ctgtttttaa ttcgctcgtg aattatttga ta
//
LOCUS       DASWKH010000038         7139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_85568, whole genome shotgun sequence.
ACCESSION   DASWKH010000038 DASWKH010000000
VERSION     DASWKH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85568"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VGA92_02865"
     CDS             complement(<1..581)
                     /locus_tag="VGA92_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845386.1"
                     /translation="MKPSLVFSLLAFSLIMPNLAFAETGTSSVDVDGTTFEVSYDATG
                     LTIDDMEADTATGTLTIFITTTDIVASLEITLERSFFDSQIDGEDVEFVVLADGFDTP
                     FEETATDTARTLTIDVPEGTNSLDIISFGATSFGSVEMPEEVPDEEQVEETPTDAPEE
                     TTEESEMQCGPGTILQDGVCVLDERCGPGTIFK"
     gene            complement(877..1518)
                     /locus_tag="VGA92_02870"
     CDS             complement(877..1518)
                     /locus_tag="VGA92_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845387.1"
                     /translation="MRHKKINEEGWRYFSTILKEYQISKNQLELAIQEGFVRVKEVPN
                     PHYTTKTSRVILENDIKNNLGRIQSLRKTSDEEKKKRKQYATRSHRSKTLENVKCPIC
                     GEKCIPEEKSKSEMLKLFKDGDVDFNGAASKVIFAHVQHAHTNYESDLKRYRQRFSKM
                     ISELMDDGCSFNDARMLVDDELEDEYRQIKNNAIDKARQLMQTFSTDLLKTST"
     gene            complement(1537..1815)
                     /locus_tag="VGA92_02875"
     CDS             complement(1537..1815)
                     /locus_tag="VGA92_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845388.1"
                     /translation="MVEHRIELSGSNVPILASMGMVENVLKKHREFASKSALQRALPK
                     TIQTQTFNRILNYLEKSNKIIFTKDRAVVWIFSTDPKLRKIWKKSVPL"
     gene            1878..2186
                     /locus_tag="VGA92_02880"
     CDS             1878..2186
                     /locus_tag="VGA92_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845389.1"
                     /translation="MGIKSVLQKKDHKHSRSAEHTPNLRTIEMVEHVLTKEKEFSSRN
                     RLSRALPKQVQQQTIEVILEYLERSNKIIRDKKGTILWIFGDNNPKLMKLHKESIELK
                     "
     gene            complement(2550..3482)
                     /locus_tag="VGA92_02885"
     CDS             complement(2550..3482)
                     /locus_tag="VGA92_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX9845390.1"
                     /translation="MSFNQSVLICDQVDSILENILKKNGLQVTSEPQITPEELAKKIA
                     SFDIVVVRSRTTITKELIEKATKCKIIARVGVGLDNVDAEFAKTKNIRVINAVEGAMN
                     AVAELVICFMLSMAREVPRADRDLRGGKWIKKELMGTELAGKYLGIVGLGNIGKRLGR
                     LARSLNMNIIGYDVIPIDQEFVKEVGLIKTDLDTLLQSSDYVSLHVPLLDSTKHMINA
                     ERMAKMKKTAKIINTSRGGTIDEDALYEALNSGNLGGAALDVFEKEPAIGNKLATLPN
                     VICTPHIGAQTKEAQSLAANVIAEKIIQILRGVI"
     gene            complement(3555..4889)
                     /locus_tag="VGA92_02890"
     CDS             complement(3555..4889)
                     /locus_tag="VGA92_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9845391.1"
                     /translation="MLFSVTAVAVLAATLFGSTYTQTQVVGTTMAASKGDNDLLQQLH
                     DMGGLKLVMPQAFASVDCDNIEEGRNVVSFDLTGESAELPVIGGGTYHAMTFSGQVPG
                     PTLRVTQGDVVMMTLTIPSDEPTPHGNDMHASEMSAGNFGKVLPGESKTYCYVAQVPG
                     TFKYHCSGVDVAAMDQHVLSGMYGITIVDPLKGYKPLLVEKTKVEDGQVVRDRQMYSA
                     DALEFQLQYNQLYLTDEGGYDQGKMFKHETTWTVVNGISFGYVPNAAHNELILGAANK
                     NIFVAQPWNSADLAQYQSQLLFIEEGQKVRFFIENQGNEPVFFHIVGEIIDRVTQANR
                     VQAQGTETWLVGGSQGMIADVVFDEPGVYVAVNHDYAAIFTGAATVMVVGDPFGLNAQ
                     LGTNAASYAHLLGNPSDAIPPMGKNSVEHPMVNLHGLYTDDRAAEIAEELGI"
     gene            5034..6395
                     /locus_tag="VGA92_02895"
     CDS             5034..6395
                     /locus_tag="VGA92_02895"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX9845392.1"
                     /translation="MEYRTDKDSLGMVKIPANAYYGAFTGRAIKQYHVTGKKSHRHLV
                     DAFVMIKRSAAVANIRCGAIDKKRGTAIIRACDKILAGKHLDQFVIEEINSGAGTAFN
                     MNANEVICNVALEVLGKKKGRYEFLHPNDHVNMSQSSNDTYPTAMHVAILLNLREMLP
                     ALDTLIKSLSKKAKQFSSYKKIGRTHLMDALPVTLGSEFAAYSISVLKAKNAIIAATK
                     ELELVALGGTAVGSGANTPRGYRKIAIVELAKVSKLNLRPEKDMQYSLQSKFAVSNVS
                     ATVRNLALELNKIANDIRLMASGPIAGFSEIGIPAVHAGSSIMPGKVNPSLAECLNMV
                     CFSIIGNDTAVANAAQAGQFELNVMLPGMLKAVLDSTDMLKNFLPIFSANLIDGLTAN
                     EQRLKQNIEKSPVIVTLLAPKIGYQKSADLYKESLKTGKTIRQIVVSQKLMTQKQVDA
                     LLK"
     gene            6397..>7139
                     /locus_tag="VGA92_02900"
     CDS             6397..>7139
                     /locus_tag="VGA92_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylthioadenine synthetase"
                     /protein_id="HEX9845393.1"
                     /translation="MAKIWIESYGCSASFADSEMISGLIVNGGHVLASSAKESDLNLI
                     VTCSVKDATANKMVNRIKKLRSKPLVIAGCLSKAEPKTVERLSSSASLMGPNSIGKTL
                     QIINATLSGRRQVELADTDLTKTGLPKVRLNPAVGIVEIASGCMSECTFCQTKLAKGD
                     LKSYRIGDIVRQVRAEVDDGCKEIWLTSTDNGCYGLDIGTTLPDLVNKVAEIPEEFMV
                     RVGMMNPMYMPKIRDGLLKSFESSKVYKFL"
BASE COUNT         2047 a         1520 c         1427 g         2145 t
ORIGIN      
        1 tctttgaata tggttccggg gccacacctt tcatctagta cacaaacgcc gtcttggagt
       61 atggttccag ggccgcattg catttctgac tcttctgtag tttcttctgg cgcgtcagtt
      121 ggcgtctctt ctacttgttc ttcatctggc acctcttctg gcatttccac agatccaaag
      181 cttgtcgcgc caaatgagat tatatcaagc gagtttgtcc cttcaggaac atcaattgtc
      241 agagttcttg cagtgtctgt cgctgtttcc tcaaatggag tgtcaaatcc gtctgctagc
      301 accacaaatt caacgtcttc accatctatc tgtgaatcaa aaaaacttct ctcaagtgta
      361 atttccaagc ttgccacaat atcagtcgtt gtaataaaaa tagtcagcgt gccagtagct
      421 gtgtctgctt ccatgtcatc gatagtaaga cctgtcgcat catagctaac ctcaaaagtt
      481 gttccgtcca catcaacact tgaggttcct gtttctgcaa atgccaagtt cggcatgatc
      541 agactaaagg ccagcaatga aaaaacaaga cttggtttca atctagatta cgcctcgcaa
      601 aatctgcatt actctcctat atttagttaa attagtcatt tgatgcgtga taattaggct
      661 caaatcattg taaaaattgc agaatttttg ataaattgtt ggataatttt attgtgattg
      721 attggtcaaa aatgtcagtt actaagtaga ggaatatgcc agattagtac atattctatc
      781 aaacaaaagt ggcaacacat tttattctta tcaggatttg cgctatttga tgacgtgttt
      841 ttggaaaatc agaaaccaat caaacaaaaa gagaaatcat gtagatgtct tcagtagatc
      901 agttgagaat gtctgcatca actgtctggc tttgtcgatg gcattgtttt tgatttgacg
      961 gtattcatct tctaactcgt catctactaa cattctggca tcattaaaac tacaaccatc
     1021 atccatcaat tcagaaatca tcttactgaa tctctgacga tatcttttga gatcggattc
     1081 gtaattggta tgtgcgtgct ggacatgcgc aaaaatcact tttgaagctg caccgttaaa
     1141 atcaacatct ccatctttaa acaatttcag catttctgac tttgactttt cctcagggat
     1201 gcatttttca ccacatatcg ggcatttcac gttttccagt gtcttagagc ggtggcttcg
     1261 tgttgcatat tgcttccgtt tcttcttttc ttcatcactg gtttttctaa gtgactggat
     1321 ccgccccaaa ttattcttaa tatcattctc taaaatgact cgcgaagttt tcgtagtata
     1381 gtgagggttt gggacttcct tcacacgtac aaaaccttct tggatggcca attcgagttg
     1441 gtttttactg atttgatatt cttttaaaat agttgaaaaa tatctccagc cctcttcatt
     1501 tattttttta tgcctcaaaa tcaaaaattc ccataattat aatggaactg atttcttcca
     1561 tatttttcgt agtttaggat cagttgagaa tatccaaaca actgcacgat ccttggtgaa
     1621 gattattttg tttgattttt ccaaataatt taaaattctg ttgaatgtct gagtttgtat
     1681 cgttttaggt aatgcccttt gcaaggcact tttggatgca aactcgcgat gtttttttag
     1741 aacattctct accattccca tacttgcaag tataggaaca ttacttccac ttaattcaat
     1801 gcggtgttct accaataatc ttagtataaa gttatactat ataaaagtat tatatataag
     1861 ttcatctaat gagtggcatg ggtataaaat cagttttaca aaaaaaagat cataaacatt
     1921 ccagatccgc agaacacact ccaaacctta gaaccatcga gatggtggaa catgttctaa
     1981 ctaaggaaaa ggagttcagt tcaagaaaca gactgtcaag agcattacca aaacaagttc
     2041 aacaacaaac catcgaagtg attcttgaat acctagaaag atcaaacaaa ataatacgag
     2101 acaaaaaagg aaccattttg tggatattcg gagacaataa tccaaaatta atgaaactac
     2161 ataaggaatc aatagaattg aaatagttca gtaaccgaat ctcttttcat attctttgtg
     2221 agtggaaaac atctccaaga tattgcaata tcttacatct gattttgtat atattgtata
     2281 gatcaaccgt ctaccttctc ttaattcgaa tcttagtaga acattcacat cgaaagcttg
     2341 tacataacac tgtggccatt tcttgcgttc tatcctttga ccacgcatac aatcttcatt
     2401 taagagagtt aaagcaccat ctatttctct gtaaagatca tcggatgtca ctaaagaatt
     2461 aaagatcttc ataaactttg gagagcccat aatttttgtt tccaaacaaa gcaaaatgtt
     2521 aatagaacga tttaatgatt attgctatcc tagattacac cacgaagtat ctgtattatc
     2581 ttttctgcta tgacatttgc ggccaatgac tgagcttctt ttgtctgggc cccgatatga
     2641 ggcgtgcata ttacgtttgg aagtgtcgca agtttgtttc cgatggctgg ttctttttca
     2701 aacacatcta gtgccgcacc cccaaggttt ccgctattta gcgcctcata caatgcatct
     2761 tcgtcaattg tgccgcccct tgaggtattg atgatttttg cagtcttttt catctttgcc
     2821 atccgttctg cgttaatcat gtgttttgtg ctatccaaaa gaggcacgtg taatgagaca
     2881 tagtcggagc tttgcaaaag cgtatccaag tctgtcttga tgagccccac ctcttttaca
     2941 aactcttgat caattggaat gacatcataa ccaatgatgt tcatattaag cgatcttgca
     3001 agccttccaa gtctttttcc aatgtttcca agccccacta ttccaagata tttccctgca
     3061 agctcagttc ccataagttc tttttttatc cactttccgc ccctcagatc cctgtcggcc
     3121 cgcggaacct cccgtgccat tgaaagcata aagcagatta ccagttctgc tactgcgttc
     3181 attgccccct ccacggcatt gatgactcgg atgttttttg tcttggcaaa ttcggcatca
     3241 acattatcca aacccacccc aactcgtgca attattttgc acttggttgc cttttctatc
     3301 agctcttttg taattgttgt tctacttctg actacaacaa tatcaaaact agcaattttt
     3361 tttgccaatt cttccggtgt tatctgcggc tcagatgtta cctgtaggcc gttttttttc
     3421 aaaatgtttt ctagaataga gtctacttgg tcgcagatca aaactgactg attaaaactc
     3481 atgtagtttg cctttttttg tacagaatat taatcaatat gggatctaat aaaaataaga
     3541 aaatggggaa gtgtttagat tcctagttct tctgctattt cggcagcacg atcatctgtg
     3601 tacaatccgt gcaagttaac catcgggtgc tcaacgctgt ttttacccat tggtgggatt
     3661 gcgtcgcttg ggttgcctaa gagatgtgca tatgatgctg cgtttgtgcc caattgtgcg
     3721 tttaagccaa atggatcgcc tacgaccatt actgttgctg cacctgtgaa gattgcggca
     3781 tagtcatggt ttactgcaac atatactcct ggttcatcaa agaccacatc ggcaatcatg
     3841 ccttgtgagc ctccaactaa ccaagtctca gtgccctgtg cttgcactcg gtttgcttgt
     3901 gtgacacggt cgatgatttc acctacaatg tggaagaata ctggctcgtt tccttgattt
     3961 tcaataaaga agcgtacctt ttgtccttcc tcaatgaaca atagctgtga ctggtattgt
     4021 gccagatcag cagagttcca tggttgtgct acaaagatgt tcttgttagc tgcacccagt
     4081 atgagttcgt tgtgggccgc atttggaaca tagccaaatg aaatgccgtt aactactgtc
     4141 catgtggttt catgcttgaa catttttcct tgatcatatc caccttcgtc agtcagatac
     4201 aactggttgt actgaagctg gaattcaagg gcatcagcgg agtacatctg cctgtccctt
     4261 accacctgtc cgtcctcaac ttttgtcttt tctaccaaca atggcttgta gcctttgagt
     4321 gggtcgacaa ttgtgattcc gtacattcca gagaggacat gctggtccat tgctgctacg
     4381 tcaacgccag agcagtggta cttgaaggta cccggcactt gggcaacata gcagtatgtc
     4441 ttggattcac ctggcaatac tttgccaaag tttcctgcgc tcatttcaga tgcgtgcatg
     4501 tcgtttccgt gtggggttgg ttcatcgctt ggaatggtaa gtgtcatcat tacgacatca
     4561 ccctgtgtga ctctaagtgt tggtcctggg acctgtccac taaaagtcat ggcatgatat
     4621 gtgccgccgc caattactgg cagttcagca ctctcgccgg tgaggtcaaa tgaaacaacg
     4681 tttctaccct cctcgatatt gtcacagtct actgaggcaa atgcctgtgg catgacaagc
     4741 ttcaatccac ccatatcatg gagttgctga agtaagtcat tgtcgccttt tgaggctgcc
     4801 atagtagtac cgacaacttg tgtctgtgtg tatgtgcttc cgaataaggt tgctgctaaa
     4861 accgctacgg ctgtaacgga aaacagcata ctgtaacgct tattcatgtg tttgagactt
     4921 gtaccacggt atataataat tcccattatt tctacgggaa ttttgatgaa attgtgtttg
     4981 taataccatt gtgacccgtt gagaatttat cttagttgta agaaggaaaa ttgttggagt
     5041 acagaacaga caaggactca cttggcatgg taaaaattcc tgcaaacgcc tactatggag
     5101 catttactgg aagggcaata aagcaatatc acgtcactgg aaaaaaatcc cacaggcacc
     5161 tagttgacgc atttgtaatg ataaagcgtt ctgcagctgt tgcaaacatc agatgcggcg
     5221 caattgacaa aaaacgtgga actgcgataa ttagggcgtg tgataagatt ttggctggaa
     5281 aacatttgga tcagtttgta atagaagaga tcaattcagg tgctggaact gcgtttaaca
     5341 tgaatgccaa cgaagtcatt tgtaatgtcg cgttggaggt cttgggaaaa aagaaagggc
     5401 gatatgaatt cttgcaccca aatgatcatg taaacatgtc gcaatcaagc aatgacacgt
     5461 atcctactgc gatgcatgtg gcaattcttt tgaatctgcg tgagatgcta ccagcacttg
     5521 acacactaat caaatcattg agcaaaaaag caaaacaatt ttcaagctac aaaaaaattg
     5581 gcagaacaca tctaatggat gcgctcccag taacattggg aagtgagttt gcagcttatt
     5641 ccatatctgt tttaaaggca aaaaatgcaa tcattgcggc aacaaaggag ctagaattgg
     5701 ttgcgcttgg cggaacggcg gtaggaagtg gggcaaacac accaagggga tacagaaaaa
     5761 ttgcaattgt cgagcttgca aaggtttcta agctaaatct aaggccggaa aaagacatgc
     5821 agtactcgct gcaatccaaa ttcgcagtgt caaatgtttc tgccacagtt agaaacttgg
     5881 cactagagct aaacaaaatt gcaaacgata tcagactcat ggcatcagga ccgattgcgg
     5941 gtttctcaga gattggaatt cctgcagtgc atgccggctc atcaatcatg ccaggcaaag
     6001 tcaacccatc gcttgcagaa tgcctcaaca tggtttgttt tagcataatt ggaaatgaca
     6061 ctgccgttgc aaatgccgct caggcaggac agtttgagct aaacgtaatg cttcctggga
     6121 tgctaaaggc agtgcttgac tcaactgata tgctcaagaa ctttttgcca atattttctg
     6181 caaacctaat tgatgggctt actgcaaacg agcaacgcct aaaacaaaac attgagaaaa
     6241 gcccagtcat tgtcaccctg cttgcgccaa agattggcta tcaaaaatct gcagacctct
     6301 ataaagagtc actaaagaca ggcaagacca ttcgccagat agtagtctca caaaagctaa
     6361 tgactcaaaa acaagtagac gcactactca agtaacatgg caaaaatctg gatagagtca
     6421 tacggctgtt ctgccagctt tgcagattct gagatgatct caggcctcat tgtaaacgga
     6481 ggtcatgttc ttgcatcaag tgcaaaggaa tctgacctaa acctcattgt cacatgttcg
     6541 gtaaaggacg caacagcaaa caagatggta aatagaatca aaaaactccg ctcaaagccg
     6601 cttgtaatag ctggatgcct atcaaaggca gagccaaaga cagttgagcg gctttcttct
     6661 tcggcaagcc ttatgggtcc aaattccatc ggaaagacgc tgcaaatcat aaatgctacg
     6721 ctttctggaa ggcgtcaagt agagcttgcc gacacagatt tgacaaagac tgggcttcca
     6781 aaggtccggc tgaatccggc tgtcggaata gtagagattg ccagtggttg catgagtgaa
     6841 tgtacgtttt gtcaaaccaa gcttgcaaaa ggcgatctca aatcatacag aattggcgac
     6901 atagtcaggc aagtaagggc cgaagtagac gatggatgca aggagatctg gcttacgtca
     6961 actgacaatg gctgctatgg gctagacatt ggaacaacac tacccgatct cgtaaacaag
     7021 gttgccgaaa ttccagagga attcatggtt cgcgtgggga tgatgaatcc gatgtacatg
     7081 ccaaaaatca gggatggtct tctcaaatca tttgaaagca gcaaagtcta caaattcct
//
LOCUS       DASWKH010000039         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3403348, whole genome shotgun sequence.
ACCESSION   DASWKH010000039 DASWKH010000000
VERSION     DASWKH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403348"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1029)
                     /locus_tag="VGA92_02905"
     CDS             complement(94..1029)
                     /locus_tag="VGA92_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9845394.1"
                     /translation="MEFLVVEDLVTHYATEKGPVEAVQNVSFSIDEGESIGIAGESAC
                     GKSTLGLSLMRTIQGGRIISGKISFDGQSVLDLSEEEFNKNYRWKKISMVFQGAMNSL
                     DPVYTIYQQFLDILKQHNYAENHDEIVKSSLQSVGLNEDVLKKYPHELSGGMKQRVVI
                     AMALLLKPKLVIADEPTTALDVLVQSQILNLFKKLKKQGVSFMLISHDLSTISEVCEK
                     IGIMYGGKMVEFGSSAEILQNPKHPYTQALIQSIPKLRGDEKPKFLAGSPPTLVNPKP
                     QCRFIDRCPQAMEKCKKEPPVLKTDSGYVLCWLYE"
     gene            1186..1659
                     /locus_tag="VGA92_02910"
     CDS             1186..1659
                     /locus_tag="VGA92_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9845395.1"
                     /translation="MMQKIIESPQVRKKLAELRQEILILEKLNKFLEMKVQKMTQVDD
                     APPSWIKIENRETILSIVTSFADWDKKQILEILKEKPLTVLEIVQRTNLSQTSTYRKV
                     NELIKDNMIKSVGLILNKEGKRVHIYSATIKHMRIYFEENDILMFLMLENNQESF"
     gene            1743..2075
                     /locus_tag="VGA92_02915"
     CDS             1743..2075
                     /locus_tag="VGA92_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845396.1"
                     /translation="MICNNRDVAETIKEFFDVCGHKCEVAADRKSAVLLLFLNKFDIV
                     IADLPLPDFDYSDLLISLKRNGTTNAKVFIVAETDLSTSTTNILREVGITGVLKKPLS
                     VRLLERML"
BASE COUNT          669 a          421 c          431 g          660 t
ORIGIN      
        1 aaaaagaaac cgggtttggg tatcaattga tagaaaagtg gttcgttctg agatcctcag
       61 aatcattgat tcgtgtcttc cgattgcaaa gagttactcg tagagccagc acaacacata
      121 gcccgagtct gttttcagaa caggtggctc ttttttgcat ttttccattg cttgtgggca
      181 tctgtcaatg aacctacatt gtggttttgg attaaccagt gttggtgggc tgcctgcaag
      241 gaattttggc ttttcatcac cccgaagttt tggaatggat tgaattagtg cttgcgtgta
      301 tgggtgtttt gggttttgaa ggatttcagc cgagctccca aactctacca ttttgccacc
      361 atacatgatg ccgatttttt cgcagacttc ggatatggtt gacaagtcat gtgaaatgag
      421 cataaatgat acaccttgtt tcttcagttt tttgaataga ttgaggattt gagactgcac
      481 taggacatcg agtgcagttg ttggctcatc tgcaatgact agttttggct ttagtaaaag
      541 cgccattgct attacgacac gctgcttcat ccctccactc aactcgtgtg gatatttttt
      601 taagacgtct tcgtttaggc caactgactg tagtgatgat tttactattt catcatggtt
      661 ttctgcgtag ttgtgctgtt ttaggatgtc taaaaactgt tgataaattg tgtaaactgg
      721 atcaagagag ttcattgcgc cttgaaaaac cattgagatt ttcttccagc gataattttt
      781 gttgaattcc tcttcagata gatcaagcac tgattgccca tcaaatgaaa ttttgccaga
      841 aatgatcctg ccgccctgta ttgttcgcat aagcgacagg ccaagtgtac tttttccaca
      901 tgcactttcg cctgcgattc caatagactc gccctcatcg atggaaaagc ttacgttttg
      961 cactgcttct actggacctt tttctgttgc atagtgagta accaagtctt ccactactag
     1021 aaattccacg atacgatcac agccgctgtt ttacttaagg atacgcgtta ctgctacaga
     1081 tggtggtttt tgatgtagtc gatcaagtac cgcattggaa cccgataacc gtgataattt
     1141 ccgagctttt tatttaaaaa ctggatcaag ttgttaacag cacaaatgat gcaaaaaata
     1201 atagaatcac cacaagttag gaaaaaacta gcggagctta ggcaggaaat tctaatttta
     1261 gaaaagctaa acaaattctt agaaatgaaa gtacaaaaaa tgacacaagt cgatgacgcg
     1321 cccccatcat ggataaaaat tgaaaatcgt gaaactatct tatccatagt cacatctttt
     1381 gctgactggg acaaaaaaca aattctggaa atactcaagg aaaaaccact aaccgtccta
     1441 gaaatagttc agcgcacaaa cctatcgcag acttcgacgt ataggaaagt caatgagtta
     1501 atcaaagaca acatgattaa aagcgtgggc ttgattctca acaaggaagg caaaagggtc
     1561 cacatttatt ccgcaacaat caagcacatg aggatctatt ttgaagaaaa cgacatactg
     1621 atgtttttga tgttagaaaa caaccaagaa agtttctaaa tcaccgtcaa gatttccaaa
     1681 aaatgacaat tgtgaagtag cgttttaagt gtataatcta gagcgggtgt atgaaaatcc
     1741 tcatgatctg taacaacaga gatgtagctg agacaattaa ggaattcttt gatgtttgcg
     1801 gccataaatg tgaagttgca gctgacagaa aatcagctgt gttactgctt tttttaaaca
     1861 aattcgacat agttattgct gatctacctt taccagattt cgattattct gatttactca
     1921 ttagcttaaa gcgtaatggc actacaaacg ctaaagtgtt tattgtagct gaaactgatc
     1981 tttcaactag cacaacaaac atcctaagag aagttggaat aactggagtt ctcaaaaagc
     2041 cactatcagt caggcttctt gagcggatgt tatgaaagta aaatcgtcca aataacattt
     2101 aaaaaaatca attccgggtt tcatagatca cattgtcctt ggtaccaacg aactcccgat
     2161 aagtttacag atcccaccta t
//
LOCUS       DASWKH010000040        12235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_87173, whole genome shotgun sequence.
ACCESSION   DASWKH010000040 DASWKH010000000
VERSION     DASWKH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87173"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="ilvC"
                     /locus_tag="VGA92_02920"
     CDS             <1..817
                     /gene="ilvC"
                     /locus_tag="VGA92_02920"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9845397.1"
                     /translation="HTVMSVAEATKKGDIVHILIPDMIQARVYREEIGPNLSKGKALS
                     FSHAAAIHWGWITAPDDVDVIMVAPKGPGSKVRETYLEGFGTPSIVAVYQDKTGTAWN
                     RILAMGKAIGSARAGLIQTTFKEEVETDWFGEQADLCGGCASMVVNAFETLVEAGYQP
                     EIAYFEVLHELKLIVDMIQKYGINGMWRRVSETARYGGLTRGPMVMDKDVKEKMKKVL
                     QMIQDGTFNEEWISDYEKNGKNSFEKYMKQLEAHQIEQVGKQMRKMMWPDSKE"
     gene            905..1126
                     /locus_tag="VGA92_02925"
     CDS             905..1126
                     /locus_tag="VGA92_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845398.1"
                     /translation="MKKNKLTFSVKKDEEGGYTAQCMEFPGIITEGDTKRELMEMIND
                     AVEGYFEAFPEEKEKLPSIQKSEVIVVTV"
     gene            1214..1324
                     /locus_tag="VGA92_02930"
     CDS             1214..1324
                     /locus_tag="VGA92_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9845399.1"
                     /translation="MTNGKYKVTIPRHNELRKGTLLSIIGQSRLDRNLFS"
     gene            complement(1340..1762)
                     /locus_tag="VGA92_02935"
     CDS             complement(1340..1762)
                     /locus_tag="VGA92_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX9845400.1"
                     /translation="MKQKLASRRVKILVAKLGLDGHDRGALVLCRAFRDAGMEVIYSG
                     LFATPERVAQIAEDEDVDAVALSLLNGAHNTLFPRVVKELHKKGLKDVLVIGGGVIPN
                     EDKSGLEKAGVSGVFGPGTPLQTIIDHINNGVAKLRKL"
     gene            complement(1796..1996)
                     /locus_tag="VGA92_02940"
     CDS             complement(1796..1996)
                     /locus_tag="VGA92_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845401.1"
                     /translation="MNNTDGLGKWIEKHANNKFSKSLFSHDRITLHSLKERIIELERM
                     RDQILYELDRLKSLAEERHLWR"
     gene            2119..4605
                     /locus_tag="VGA92_02945"
     CDS             2119..4605
                     /locus_tag="VGA92_02945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9845402.1"
                     /translation="MQVKPINYTLTFEPHFKNFTFSGNEIIDVKISKPTNTINLHAAE
                     LKIKNCHIIWAGQKITARTRLDEKTESLQIKTSKKIKGQAKIFIDFIGILNDRLVGFY
                     RSQYKDRQGKTKFLTTTQFEAADARRAFPCWDEPAIKATFDISIITETNHTAISNTNV
                     ISKKKLGSKTLYKFAKTPIMSTYLVYLGVGEFEFLSGKLGKVTIRIVTTKGNSKRGRF
                     ALDLCKELVKSYENYFGIRYPLPKLDLIAVPDFASGAMENWGAITFRETILLYDPRTS
                     STRTKQYIAEVVSHELAHQWFGNLVTMQWWNDLWLNESFATFMATKFVDKFYPEWKLW
                     DQFLEDTMNAAMGLDALHSSHPIDVKVSSPSEIREIFDAISYDKGGCVLRMLESFVTE
                     KMFRAGLRAYLKKFAYKNAKGGDLWDEIGKAAKMPVRTMVNSWLRQVGFPLLEAQKLD
                     SKLVLRQKRFVHEKKGKKNGLWHIPVSITQDNKTTKKLVTKTQDIIPIRQMPLVINSG
                     RTGFYRVKYSDELLDDLKTLILEKSISHVDRWALQNDLFALCISGDGTCRGYLDFTTA
                     YENEDDYITRSNIASNLYFLYQKTRDESFSYEIKEITHNFFKNIFYQVGWDAKENEPH
                     TNALLRSFILSALGKLDDDEIITEANKRFEQYLKNPLSVKPDIQDPIFSIVAWSGDSR
                     TYQKLLGLYRTVKTQEEKIRLLGALCNFKDEKLLLDTLKFSQSKEVRSQNMHLPIMRT
                     AANPYGKKILWPWLKKNWKSIRAKVGAGSPLLNRIVSSISAIADASMEREIRQFFRAN
                     PTPGTERTLEQTLERIRIHSALLQRMRQEF"
     gene            4608..5012
                     /locus_tag="VGA92_02950"
     CDS             4608..5012
                     /locus_tag="VGA92_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845403.1"
                     /translation="MNKKIALAAIGTVIAFGIGISISQSIEIQEKYSKVFYFDAVFYE
                     DKKYVEITFSDDTQKTTNTVLEILGMEESFQKTFEGWNFKTTVPFEQEPEYGWKVVPV
                     TLVVNHPEFGKVGIKTEIHKEGMQGKVIFSQL"
     gene            5055..5972
                     /gene="meaB"
                     /locus_tag="VGA92_02955"
     CDS             5055..5972
                     /gene="meaB"
                     /locus_tag="VGA92_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9845404.1"
                     /translation="MELLSELKKGKRGAIAKAISIIENDPAQAKKITKKIFKTTGNSI
                     IIGITGPAGAGKSSLINKTSLALKKLGFKAAVLAVDPTSHITGGAILGDRVRMTESTD
                     SGTYIRSIASRGATGAVSRSIRSSIRILEYAGFNPIIIESVGAGQTEVEIASIADITV
                     VVFNPNTGDSIQTIKAGITEIGDIYLVNKSDLDGASQLYDSVKEFIGMTEKNPIILQT
                     SATKNKGIEDLAKKLVELMKEKKKSKKEHDKMRLEAELKDIVLNNVKHKIDSLLESDK
                     NYAKYLKKLQSKEMDPFEAADKISGSILK"
     gene            5978..7570
                     /locus_tag="VGA92_02960"
     CDS             5978..7570
                     /locus_tag="VGA92_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9845405.1"
                     /translation="MAQIEKQKPKEILTDSNFPVSRIYSQSAKKKPKEDSGKYPFTRG
                     IHPEMYRERLWTMRQYSGFGDAKQTNERFKFMLEKGQTGLSMAFDLPTQIGHDPDAPQ
                     AEGEVGKVGVSIASLKDMLTAFDGIPLGKVSTSMTINSTASTLLAYYIAVGESQGFKS
                     VDLRGTTQNDILKEYIARNTYIYPPQPSMRLIGDMIAYCAKSVPQWYPISISGYHMRE
                     AGSTATQEVAFTIANAIAYIQTCLDRGLKIDDFAPRLSFFFCCTIEFFEEVAKFRVAR
                     KVYAKILKEKFHAKDPKSIQLKFHTQTSGESLTAQQPDNNIVRVAIQTMASVLGGTQS
                     LHTNSRDEALALPTAESAKIALRTQQIVAHESGITKTVDPMAGSYYLEYLCDEIEDGV
                     WKYLKQIEKMGGALKAIEKGFFQSEIRQNAYRLKKEVDSGERVLVGVNKFSDNAESKQ
                     ELLRIDESVGEQQKKAMKKLRAERDNKKVGYTLSKLKAVAEKNENLMPYILDSVRVYA
                     TTGEISNTFREVFGEYRPKEVF"
     gene            7570..7965
                     /gene="mce"
                     /locus_tag="VGA92_02965"
     CDS             7570..7965
                     /gene="mce"
                     /locus_tag="VGA92_02965"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9845406.1"
                     /translation="MKIDHIAIAVNNVEEAAKKYQQALGVDKVIFETIESEGVKLAIL
                     KLENARIELMQPTRDDSPIKKFLEKKGEGLHHMALATNDIEAEYKRMEGCGIQFLGKI
                     RDGSEGTKITFIHPKSLSGVLAELCSHPH"
     gene            8022..8414
                     /locus_tag="VGA92_02970"
     CDS             8022..8414
                     /locus_tag="VGA92_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845407.1"
                     /translation="MSTFEALVEKKGNLFILCVFAAIGIMFLPVILPHIFHGSHIIHI
                     GLHVAGLALAIFLTISTTYAYVKIRTKRLATAAIAFSLFIAAEVFAVIEATWPFAFYI
                     DAVSMLEISHLLIIGMLGIFSLGVFKRD"
     gene            8416..8910
                     /locus_tag="VGA92_02975"
     CDS             8416..8910
                     /locus_tag="VGA92_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9845408.1"
                     /translation="MERVEQIVHLVNANPGIRYSEIMMETGLKNGVLSHHLSKIEQAG
                     RIIIERTPRVARIYPCGTSQQESTIIKNLRNATTQRILVALLEEDLTFKELVGRAKKS
                     QGTVSLSLKKLCEEGIVRRKLDDGLSVFQLTNKSLLDSLIDKHKPSFIENSANNISDI
                     FSSL"
     gene            complement(8921..9478)
                     /locus_tag="VGA92_02980"
     CDS             complement(8921..9478)
                     /locus_tag="VGA92_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845409.1"
                     /translation="MLPRLESIKQARTKLGITQKELARMAGISTSMINQIESGRSQPS
                     YETAKRIFDGLATLEGRSDPNKAGDICSKDIVRLKPVDSLHDAIHKMRKLSISQIPIF
                     NGSEPVGVITEDGVVKHLTDSDESSWKRIRLADVMESRPPIVDHQTPAKALVPLIRYS
                     KCILVAKTGKIIGIITASDTLKMLE"
     gene            9614..10531
                     /gene="tfb"
                     /locus_tag="VGA92_02985"
     CDS             9614..10531
                     /gene="tfb"
                     /locus_tag="VGA92_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966100.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HEX9845410.1"
                     /translation="MIMQTLNVNGRCSRCKGLMLTDGTTGERYCESCGFVIIERLEDA
                     RPERRSFVDDHDNASRTGAPSSLAIHDRGLATMIGYVNKDSTGKTLSVSMKQNINRLR
                     IWDGRSQTQENTDKNLKQAFMELGKLKDKLTLPDAVVEKTAHIYRKAMAKKLVRGRSI
                     PSVLAAATYAACRDAGMPRTLNDVSDALNIKRKDISKNYRMLVNELDLRMPLVDSISC
                     MSKIASRVGVGEKVKRYALEILRKANSMEITAGKDPTGLAAAALYISCVKHNVKISQR
                     EISIASGVTEVTIRNRHRGLRESLDLILK"
     gene            complement(10534..11400)
                     /locus_tag="VGA92_02990"
     CDS             complement(10534..11400)
                     /locus_tag="VGA92_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HEX9845411.1"
                     /translation="MLLIASISVIGAANAHKSEVIGDYKVEIGWDKEPPIVGMNNKIT
                     VMITHASPEEKAAAGEATHDEAEHEHAEGISGLASSMDVTVTLNGEKTALIMTEDEEI
                     PGLYLGEFTPPASGFPTVHLFVEIDGEPLEIDMHPEKTEDGAVIRTATSDGSINVDVI
                     TTTPTADEDMLIKIEFTDMHGNPIEHVNYDIVAIQDGSAVLTESTAHSHEGDTKHSTE
                     TLRSSDPVDIQVTILGIGLPDDEVNWSGPMGDTISLNVVPEFGPIAMIVFAVATVSIV
                     AMAAKSKIIPKL"
     gene            complement(11617..12144)
                     /locus_tag="VGA92_02995"
     CDS             complement(11617..12144)
                     /locus_tag="VGA92_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845412.1"
                     /translation="MPQSEQVHGYIFLSYDMLGTKPVPIRTIGFVRVDYQQLIVIKRE
                     KCVPKLRQDHKSLVKKLIDHFVSSGLEIQYANYTGYEKPFVITRHAPDVIAFDRTKQL
                     GYIGEAEMCNEITEQLTKEKFEDFSKKLMRKGKSEHTRLPFFIASPHECNSRIPQILR
                     DLGINERDNIHVLSF"
     gene            12095..>12235
                     /locus_tag="VGA92_03000"
     CDS             12095..>12235
                     /locus_tag="VGA92_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEX9845413.1"
                     /translation="MSYERKMYPCTCSDCGKQSEVPFQPKQDRPVYCKECFPKHQQPR
                     FQR"
BASE COUNT         3961 a         2456 c         2597 g         3221 t
ORIGIN      
        1 ccataccgta atgtcagttg cagaagccac aaaaaaaggc gacatcgttc acattttgat
       61 tcctgatatg attcaggcca gagtttaccg cgaagaaatt ggcccaaacc tatcgaaagg
      121 aaaggcatta tcgttttctc atgcagctgc aatccattgg ggttggataa cggcaccaga
      181 tgatgtggat gttatcatgg ttgcaccaaa ggggcctgga tcaaaggtta gagaaacata
      241 cctggaggga tttggaacac catcaattgt cgcagtatac caagacaaga caggtactgc
      301 atggaatcga attttagcaa tgggaaaagc aattgggtct gcacgtgctg gactaatcca
      361 gactaccttc aaagaagaag tagaaacaga ctggtttgga gaacaagctg acctttgcgg
      421 tggatgtgct tcaatggttg tcaatgcgtt tgagacgcta gtagaagctg gctatcagcc
      481 ggaaattgca tactttgagg tgttgcacga actcaaacta attgtagata tgattcaaaa
      541 atacggaata aatggaatgt ggcgaagggt gagcgagact gcaagatatg gtggactcac
      601 aagaggccca atggtcatgg ataaggacgt caaagaaaag atgaaaaagg ttctgcagat
      661 gattcaggac ggcacattca acgaggaatg gatctcagac tatgaaaaaa acggtaagaa
      721 ctcgtttgaa aaatacatga agcagcttga ggcacatcaa atagaacaag taggcaagca
      781 aatgcggaaa atgatgtggc ctgactctaa agagtaattt ctgccacgtt ttggtaccta
      841 tccaattaag ttttacaaaa caaaccaatc aaactttaat tagaatatgc gaataaagta
      901 taacatgaaa aagaacaaac tgacctttag tgtcaaaaaa gacgaagaag gcggttatac
      961 tgcgcaatgc atggaatttc caggaattat aactgaggga gatacaaaac gggaactaat
     1021 ggaaatgata aatgatgccg tggaaggcta ttttgaggca tttcctgaag aaaaagaaaa
     1081 actaccttcg atacaaaaaa gcgaagtgat agtagttacc gtctaaactg ccagttcttt
     1141 cttggagaga tgttcttaag attttgcgga aaactggatt ttggcaaacc ggcagactgg
     1201 tagtcatatc ttcatgacca atgggaaata caaagttaca ataccaaggc ataatgaatt
     1261 aagaaaaggt acactcctct cgatcatagg gcagtcgaga ttagatcgaa atttattttc
     1321 ataagtatga aaaagttttc taaagttttc ttagttttgc gacaccattg ttgatgtgat
     1381 caattattgt ttgtagtggt gttccaggac caaacactcc tgagactccc gctttctcta
     1441 gcccactctt atcctcgttt ggtattacgc ccccaccaat tactagaacg tctttgagcc
     1501 cttttttgtg gagctctttg actacgcgag gaaagagcgt attatgggcg ccattaagca
     1561 aactcaaagc aacagcatcg acgtcttcgt cttctgcaat ctgggcaaca cgttcgggtg
     1621 tggcaaaaag tccagaataa attacttcca ttcctgcatc tctaaaggcg cggcacaaaa
     1681 ccaatgcccc tctatcgtgg ccatcaagac caagcttggc aacaagaatc ttgactcgcc
     1741 ttgatgcaag tttctgcttc atttgaagag taattgatcg gttggatttt aaaacttacc
     1801 tccagaggtg ccgctcttcg gctagcgact ttagcctgtc aagctcatat agaatctggt
     1861 cgcgcattct ctcaagctca atgattctct cctttaggga gtgtagcgta atgcggtcgt
     1921 gagaaaaaag cgatttggaa aatttgttgt tggcatgttt ttcaatccat ttaccaagtc
     1981 catctgtgtt gttcatggga tccgaatttt ttacgaacaa taaaaccaat cccatgcatt
     2041 ccaagctaag cactagctaa gatcaaaata atagaatttt tctaaatata cataaagcca
     2101 gtcaaataag caaatttcgt gcaagtaaaa ccaataaact acactcttac atttgagcct
     2161 cactttaaga atttcacgtt ttcaggaaac gagataattg atgtaaaaat ttccaaacct
     2221 acgaacacaa taaatctgca cgcggcagaa ctcaaaataa aaaactgcca catcatttgg
     2281 gctggccaaa aaataaccgc aagaacaagg cttgatgaaa aaaccgaatc ccttcaaatc
     2341 aagacctcaa aaaaaatcaa ggggcaggca aaaatcttca tcgactttat tggtattcta
     2401 aacgacaggc ttgtcgggtt ttatcgtagc cagtacaaag acaggcaagg aaaaaccaag
     2461 tttttgacaa caacacaatt tgaggcagcc gatgcgcgaa gggcctttcc gtgctgggat
     2521 gagcctgcaa taaaggctac ctttgatatc tcgataatta cagaaacgaa tcacactgca
     2581 atctcaaaca ctaatgtgat atcaaagaaa aaacttggaa gcaaaacact gtacaagttt
     2641 gccaaaactc caatcatgtc tacatatctt gtttaccttg gggtcggaga gtttgagttt
     2701 ctttcaggca aacttggcaa agttacaatc agaattgtca ccactaaggg aaatagcaaa
     2761 agaggaaggt tcgcactgga cttgtgcaaa gaactggtaa aatcatatga aaattatttt
     2821 ggaataagat acccgctgcc aaagcttgac ttaattgcgg ttccagattt tgcatcgggt
     2881 gccatggaaa actggggcgc aatcacgttt agagagacta ttttgcttta cgacccaagg
     2941 acatcgtcaa ctcggacaaa acaatacatt gcagaggtgg tatcgcacga gcttgcacat
     3001 cagtggtttg gaaacctagt cacgatgcaa tggtggaatg atttgtggct aaatgagagc
     3061 tttgccacgt ttatggcaac caagtttgtc gacaagtttt atccagaatg gaaactatgg
     3121 gaccaattcc tagaagacac gatgaatgct gcaatggggc ttgatgcact acactcatcg
     3181 cacccaatcg acgtcaaggt aagcagcccc tcagaaatcc gagaaatctt tgatgccatc
     3241 tcttatgaca agggcggatg cgtcctaagg atgcttgaga gctttgttac ggaaaaaatg
     3301 tttcgcgcag gcctgcgtgc atatttgaaa aaatttgcat acaaaaatgc caaaggtggt
     3361 gatctgtggg atgagatagg caaggcagca aaaatgcctg ttaggacaat ggtaaattca
     3421 tggctaagac aggttggatt tccacttctt gaggcgcaaa aactggactc aaaactcgtt
     3481 ttgcgtcaaa aacgcttcgt tcatgaaaaa aagggaaaga aaaatggcct gtggcatatc
     3541 ccagtctcaa taactcaaga taataaaaca acaaaaaaac ttgttacaaa aacacaagac
     3601 atcatcccaa tacgacaaat gccgctagtg atcaattctg gcagaactgg attttatcga
     3661 gtcaaatatt cagacgaact actcgatgat ctaaaaacac taattttgga aaaatcaata
     3721 tcgcatgtag ataggtgggc actgcaaaac gatctatttg cactgtgcat ttctggtgat
     3781 gggacatgca ggggctatct tgactttacg actgcatacg aaaatgagga tgactacatc
     3841 accagatcaa acattgcgag caacctgtac tttttgtacc aaaaaacgcg agacgagtca
     3901 ttctcttatg aaataaaaga aataacacat aattttttca aaaatatttt ttaccaggta
     3961 gggtgggatg caaaagaaaa cgagccacac acaaacgcat tgcttagaag cttcattctc
     4021 agcgcacttg ggaaactaga tgatgacgaa ataatcactg aggcaaacaa acgatttgag
     4081 cagtatctga aaaatccctt atcggtaaag ccagatattc aggatccaat attttcaatc
     4141 gttgcgtgga gtggcgattc taggacatat caaaaactac taggtctata cagaactgtc
     4201 aagacccaag aagaaaagat tcgactcctt ggggcattat gcaacttcaa agacgaaaaa
     4261 ctcttacttg acacactgaa attctcacaa tcaaaagaag tccgctcaca aaacatgcat
     4321 ctgccaataa tgcgaactgc cgcaaatcct tacggcaaga aaattctttg gccgtggcta
     4381 aaaaagaatt ggaaatccat acgcgccaag gttggtgcag gaagtccgct tcttaatcga
     4441 atcgtatcga gcatatccgc aatagctgac gcatcaatgg agagagaaat caggcagttt
     4501 tttagggcaa atccgacacc tggaaccgaa agaacactag agcaaaccct tgaaagaata
     4561 cgaattcact cggcattatt acaaagaatg aggcaggagt tttgagattg aacaaaaaaa
     4621 tcgctcttgc cgcaattggc acggtaatcg catttggaat aggcatctca atttcacaaa
     4681 gcatcgagat tcaagaaaaa tactccaagg ttttttattt tgatgcggta ttctacgagg
     4741 acaaaaaata tgtagagatt actttttctg atgatactca aaaaacaaca aacactgttc
     4801 tggaaatact gggaatggaa gagtcatttc agaagacctt tgaaggatgg aattttaaaa
     4861 ccactgtccc atttgagcaa gagccagaat atgggtggaa ggtagtgcca gttacacttg
     4921 tggtgaatca tcctgaattt ggcaaggttg gaataaaaac agagattcac aaagaaggaa
     4981 tgcaaggcaa agtgatattc agccagcttt gacattcaac aagaatttat tgtagataca
     5041 aagagtaaga ctgtttggaa cttttatctg agctaaaaaa aggaaaacgc ggtgccattg
     5101 caaaggcaat ctcaattata gaaaacgatc cggcccaagc aaaaaaaata acaaaaaaga
     5161 ttttcaaaac tactggcaac tcgatcataa ttggaattac ggggccggca ggtgcgggaa
     5221 aaagctctct aattaacaag acctcgcttg cactaaaaaa actcggcttc aaggcagctg
     5281 ttctggcagt tgacccaaca agccacatta ccggtggtgc aattttggga gatagagtaa
     5341 ggatgacaga atccacagat tctggaacat acatccgaag cattgcatca cgaggagcaa
     5401 caggtgcagt ttcgcggtcg attcgaagta gtattcgaat tctagaatat gccggattca
     5461 atccaataat tattgaaagt gtcggcgcag gccagaccga agtagaaatt gcaagcattg
     5521 ccgacatcac tgtggtagtc tttaatccca acactggtga tagcatccag acaatcaagg
     5581 caggaattac ggaaattggc gatatttacc ttgtaaacaa aagcgaccta gatggggcat
     5641 cgcagcttta tgactccgta aaagaattca tagggatgac tgagaaaaac ccgataatct
     5701 tgcaaacttc ggctacaaaa aacaagggaa ttgaagatct tgcaaaaaaa ctagtcgagc
     5761 ttatgaaaga aaagaaaaaa tcaaaaaaag aacacgacaa gatgcgactt gaagctgagc
     5821 tcaaagatat tgttttaaat aatgtaaaac acaaaattga ttcactcttg gaatcagata
     5881 aaaattatgc caaatatcta aaaaagcttc aatcaaagga aatggaccca tttgaggcag
     5941 cagacaaaat atctggctcg atactaaagt gatacacatg gcacaaattg aaaaacaaaa
     6001 accaaaagaa attcttacag actctaactt tccagtaagt aggatctatt cccaatcagc
     6061 taaaaagaaa ccaaaagaag attctggcaa atacccgttc actcgaggaa tccatcctga
     6121 gatgtaccga gaaaggctct ggactatgcg acagtattct ggttttggag atgcaaagca
     6181 gacaaatgag cgcttcaagt tcatgctcga aaaagggcag actgggcttt caatggcatt
     6241 tgatctccca acgcaaattg ggcatgaccc agatgcgcct caggcagagg gagaagtagg
     6301 aaaagttggt gtgtcaattg cgtcgctaaa ggacatgctt actgcatttg atgggattcc
     6361 acttggcaag gtaagcacat ctatgaccat taattctact gcatcaactc tgttggcgta
     6421 ctacattgca gttggagagt cgcaaggatt caagagcgta gacctcagag gcacaacaca
     6481 aaacgacatt ctcaaagaat acattgcaag aaacacctac atctatccac cacaaccctc
     6541 aatgagatta attggagaca tgattgcata ttgcgcaaaa tctgtcccac agtggtatcc
     6601 aatttctatt tctgggtacc atatgaggga ggctggctct actgcaacac aagaagtcgc
     6661 ctttacaatt gcaaatgcaa ttgcatacat ccagacctgc cttgaccgcg gtttaaagat
     6721 tgacgacttt gctccaaggc tttcgttttt cttttgttgc actatagaat tctttgagga
     6781 ggttgccaaa tttcgagtag cgcgcaaagt ctatgccaag attctaaaag aaaaatttca
     6841 cgcaaaggat ccaaaatcaa tacagctaaa attccacacc cagacaagcg gcgagtcact
     6901 tactgcacaa cagccagaca acaatatcgt acgcgttgcc atccagacaa tggcctcagt
     6961 tcttggcggc acacaatcac ttcacacaaa ctctcgcgat gaggcacttg cactcccaac
     7021 tgcagaatct gcaaaaattg cactgagaac acaacaaata gtggcacatg aaagcggaat
     7081 tacaaagaca gtagatccga tggcgggctc ttattatctt gaatatctat gcgatgaaat
     7141 tgaagacggt gtctggaagt acctaaaaca aattgaaaag atgggcgggg cgctaaaggc
     7201 aatagaaaag gggtttttcc agtcagaaat acgccagaat gcctaccggc tcaaaaaaga
     7261 agttgactca ggtgagcgag tcctagttgg cgtaaacaag ttttctgata atgccgaatc
     7321 aaagcaagaa ctgctcagaa ttgacgagtc ggtaggagaa cagcaaaaaa aggcaatgaa
     7381 aaaactccga gcagaacgtg acaacaaaaa ggtaggatat acattatcaa aactaaaggc
     7441 cgtagcagaa aagaacgaaa acctcatgcc atacatactg gactctgtca gagtgtatgc
     7501 aactactgga gagatcagca acacgtttcg ggaagtgttt ggcgaataca ggccaaagga
     7561 agttttctaa tgaaaattga tcacattgca atcgcagtaa acaatgtaga agaggcagca
     7621 aaaaaatacc aacaggcact tggagtagac aaggtgattt ttgagacgat agaatcagaa
     7681 ggagtcaagc ttgccatact gaaattggaa aatgcaagaa ttgagctgat gcaaccgaca
     7741 cgagatgaca gcccaattaa gaagtttttg gaaaaaaaag gagaaggact acaccacatg
     7801 gcacttgcca caaatgatat tgaagccgaa tacaaaagaa tggaaggatg tggaatccag
     7861 tttttgggta aaatacgaga cggctctgag ggaaccaaaa ttactttcat tcatccaaaa
     7921 tcgctgagcg gagttttagc agagctctgc tctcacccac actagcatag tttgatagtt
     7981 ctaccagaaa ttctttaatt ttgtatgtgg cactttggtt gttgtctacc tttgaggctc
     8041 tggttgaaaa gaagggtaat ctgtttatcc tttgcgtgtt tgccgcaatt ggaataatgt
     8101 ttcttccagt aatacttcca cacatatttc atggctcgca tataatacac atcggattgc
     8161 atgttgctgg attggcactt gcaatctttc ttaccatatc caccacatat gcatatgtaa
     8221 aaatccgaac aaagcgtctg gcaactgccg caattgcatt ttcactgttt attgcagcag
     8281 aagtatttgc agtaattgag gcaacgtggc catttgcatt ttacatcgat gcagtttcaa
     8341 tgcttgagat tagtcatttg cttattatag gaatgcttgg tatattctct ttaggagtgt
     8401 tcaaaaggga ttaagatgga acgagtcgaa caaatagttc atctggtaaa cgcaaaccca
     8461 ggcatacgtt attctgaaat aatgatggag actgggctaa aaaacggcgt tctgagtcat
     8521 catctatcca aaattgaaca agctggcaga attataattg agcgaactcc gagggttgca
     8581 agaatctatc catgtggaac ctcacaacaa gaaagcacaa taataaaaaa tcttagaaat
     8641 gcaacaacac aaagaatact tgtagcactt cttgaggagg atttgacatt taaagaattg
     8701 gtaggcagag ccaaaaaatc acaaggaact gtatcactgt ccctcaaaaa actatgtgag
     8761 gaaggaatcg ttcgccgtaa actggatgat gggctatctg tatttcagct aactaacaaa
     8821 tcattacttg actctttgat cgacaaacac aaaccatcat ttatcgaaaa ctctgcaaac
     8881 aacatttcgg acatcttcag ctcgctctaa actcaacaaa tcattctagc atctttaatg
     8941 tgtcagatgc agtaatgatt ccaattattt tgcctgtctt tgcgacaagg atgcattttg
     9001 agtacctgat taacggcaca agtgcctttg caggcgtttg atggtccaca attggcggcc
     9061 ttgactccat tacgtcggca agccgaattc tcttccatga cgactcatcg ctgtccgtga
     9121 ggtgtttgac tacgccgtct tctgtgatta cgccaactgg ttcactacca ttaaagattg
     9181 gaatctggga aattgagagc tttcgcatct tgtgtattgc atcatggagc gaatcaaccg
     9241 gttttaacct gacaatatcc ttactgcaaa tatctcctgc cttgttagga tcggatctgc
     9301 cctcaagcgt tgctaggcca tcaaaaattc tttttgcagt ctcgtagctt ggctggcttc
     9361 gaccagattc tatctggttt atcatcgatg tgcttatgcc tgccattctt gcaagctctt
     9421 tttgtgttat tccaagctta gttcgtgcct gcttgatact ttctagtcgt ggaagcatga
     9481 tcttgtttca aagattaatc ctatgaagtt tacgtaaaac aacatacgcc cagaattctc
     9541 caatttgacg agagatgcca ccgtatacac tagttcttat tagtgagcgg cctaacttgg
     9601 gtgcctgatt ttgatgatta tgcaaacttt gaatgtgaat ggtcgatgca gccgatgtaa
     9661 aggactcatg ctaaccgatg gcactactgg tgagcgatac tgtgaaagct gcggttttgt
     9721 tattatagaa cgtcttgaag atgcgcgccc tgaacgtcgg tcatttgtag acgatcatga
     9781 taatgctagc agaaccggtg caccaagctc acttgccatt catgacaggg ggcttgctac
     9841 aatgattggt tatgtgaaca aggattcgac tggaaaaaca ctttctgtat ctatgaaaca
     9901 gaacatcaat cgacttcgaa tttgggatgg taggagccaa acacaggaaa atacagacaa
     9961 gaatctaaag caagcattta tggagttggg caaactaaag gacaaactta cattgcctga
    10021 tgcagtagtt gaaaaaactg cccatattta tcgaaaggcc atggcaaaga aacttgtcag
    10081 gggcaggtcc attccatcgg ttcttgccgc agcaacatat gcggcatgta gagacgcggg
    10141 aatgccaaga actctaaacg atgtttcaga cgcactcaac ataaaaagaa aagatatttc
    10201 aaaaaactac cgaatgcttg tcaacgaact ggacctaagg atgcctcttg tagattcgat
    10261 tagctgcatg tctaaaatag caagcagggt tggggtgggt gaaaaggtaa agcgatatgc
    10321 tcttgaaatc ttgagaaaag caaacagtat ggaaattacg gcaggaaaag accctacggg
    10381 gcttgcagct gcggcattgt acatttcatg cgtaaaacac aacgtgaaaa tatcgcaaag
    10441 agaaatctcg attgcttctg gtgtgaccga agtgacaata cgaaacagac accggggatt
    10501 aagagaatcc ctggatctga tcttgaaata aaattaaagt tttggaatga tttttgactt
    10561 ggcagccatg gcaacaatgc ttaccgttgc aactgcaaac acaatcatgg caattgggcc
    10621 aaattctggt acaacattta gcgatattgt atcacccatc ggcccactcc agtttacctc
    10681 atcgtctggt aatccaattc caagaatggt cacttggatg tctacaggat cacttgatct
    10741 tagggtctca gtagagtgtt ttgtgtctcc ttcatgtgag tgtgcagtgc tttcagttag
    10801 gactgcgctt ccatcctgta ttgcgacaat gtcataattg acatgctcaa ttgggttgcc
    10861 atgcatatct gtgaactcga ttttgattaa catgtcttcg tcggcagttg gggttgtcgt
    10921 gataacgtcg acgtttatgg aaccatctga tgtcgcagtt ctgatcactg ctccgtcttc
    10981 tgtcttttct gggtgcatgt caatctctaa tggttctccg tcaatttcta caaaaagatg
    11041 aactgttgga aatccagatg cgggcggtgt gaattcaccc aagtataatc caggtatttc
    11101 ttcatcttca gtcattatga gtgctgtctt ttcaccattt aatgtgactg taacatccat
    11161 gcttgaagca aggccgctga ttccttcagc atgttcatgc tctgcctcat catgtgttgc
    11221 ttcccccgcg gctgcttttt cttcggggct cgcatgtgtg atcatcacag taatcttgtt
    11281 gttcattcca actattggtg gctctttatc ccatccaatt tcgactttgt agtccccaat
    11341 aacctctgat ttatgtgcgt tggcggcgcc tatcacacta attgaagcta tcagtagcat
    11401 caatacaaga aatttgattt gcacattatg atctatggtg gatatttttt taacagtttt
    11461 ggtagaaatg tcaaacaaaa ttcgtttatt ttgatcatac ttcaatttta gtgaaacaaa
    11521 acattcgatt tttccagctc tctgcttttt gttgtaactg atggcaagaa aatatttggc
    11581 gttagtattc aggactggct ttctcagaat atgtggttaa aagctcagca catgtatgtt
    11641 gtctctttcg tttattccca aatctctgag gatctgggga attctggaat tacattcgtg
    11701 cggggatgct atgaaaaacg gaagtcttgt gtgctcggac ttgccttttc tcatcagttt
    11761 tttggaaaaa tcctcaaact tttcctttgt tagctgctct gtaatctcgt tgcacatttc
    11821 tgcctcgcca atgtaaccaa gctgttttgt acgatcaaac gcaattacat ctggcgcatg
    11881 tcgagtaatt acaaacggtt tttcatatcc agtataattt gcgtactgga tctcaaggcc
    11941 gctagagaca aaatgatcaa ttagtttttt gactaaagat ttgtgatctt gtcttaattt
    12001 tggcacgcac ttttctcgct ttattactat taattgttgg taatcaacac gtacaaatcc
    12061 aatggttctt attgggaccg gtttggtgcc aagcatgtca tatgaaagaa agatgtatcc
    12121 atgcacttgc tcagactgtg gcaagcaatc agaggtacct ttccaaccaa aacaagacag
    12181 acctgtctac tgtaaagagt gttttccaaa gcaccaacaa ccaagatttc aaaga
//
LOCUS       DASWKH010000041        32587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1192680, whole genome shotgun sequence.
ACCESSION   DASWKH010000041 DASWKH010000000
VERSION     DASWKH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 32587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32587
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192680"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VGA92_03005"
     CDS             complement(<1..718)
                     /locus_tag="VGA92_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845414.1"
                     /translation="MNVNKAQNDSYFIRIRKTNPFNFSKFVLNKNESADHADSKQILN
                     TSETRFAKIASQQDSPINEIENVLVPKFKTLNKIDFEDAEIYTGLIADTTSRNGLRYM
                     IIEPQMNKSDNVNFRVIKKLLISELDISLNSIKTKKEAAKELKSKILHLIKKYNIDIQ
                     TKSLSKITYYAIRDFVYMGKIEPLMRDHMIEEISCDGTNVPLYIWHREFESVPTNIVF
                     ENEDDLNNFVQKLSYIGGKHA"
     gene            844..1956
                     /locus_tag="VGA92_03010"
     CDS             844..1956
                     /locus_tag="VGA92_03010"
                     /inference="COORDINATES: protein motif:HMM:NF037270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845415.1"
                     /translation="MFQLNRNHSKLKGAQSADQKIPKLAQKPALVDDDLPEIKELIRW
                     NEENKQKISKLLDKIKQNFNGVLIPQIDHNKGAVHYPALGEIGELTTNPVFLEKLSHR
                     KLGFLEKFVYNQFLICPDHRASFLLNVRLCCPKCNSIDVHKLHLFEHKICGCIMEKPK
                     FVDAQEADKLKCPSCNRVIKNPQKELRSPAMWYFCNDCKEKFDEVLINFHCKEFDHDF
                     TINDAQTVTVCGYALTDGSEHSPLDYQELKSEISRVLSKLGFSVDENYTIKGKSGHDH
                     KIDIYGSNNKNQTAFILVNDSNDSEIDSKIIQILDTSPKIALIIGAASVNEKTKAIAS
                     KYNVSIISSQNMSEIVSEAEKILTYRLQKLDGVQLR"
     gene            1953..2459
                     /locus_tag="VGA92_03015"
     CDS             1953..2459
                     /locus_tag="VGA92_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845416.1"
                     /translation="MNLKRYATCTKKRGVSQIIGTMFMLALVVPVGTVIVTKGLNEVG
                     EMSNRLTSGITYQNEGGREDIVFEHIRLDPTGNQVTITLRNAGTIDTTINKITMVKID
                     TQELLISDKSLAVDAQPRIGTDILSTASLQFSGRWDDPNYVNSEYKISILTSKGNFFE
                     TVARPFNT"
     gene            2456..3124
                     /locus_tag="VGA92_03020"
     CDS             2456..3124
                     /locus_tag="VGA92_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9845417.1"
                     /translation="MRQKLLQAKRRGISEIIATLLLLGITTAGSVFLATVVQGSGLAS
                     IQGSAVTPVSPPYSIRLTGYDTRDGANLIDLNTLDNKSDKKLCTISCSLTPSNIPEND
                     GTDFIAIQIKNVSTNPVFIKNIQINGVTHDWDAQTGTKTFDASADNLLGNYPLDGKFS
                     IVSSNGVQKSDNRIVDDEEVRLVVKLSKDIASDVLLSKPIQVLVDFGGTRSTEFVVLS
                     GDAK"
     gene            3130..4851
                     /locus_tag="VGA92_03025"
     CDS             3130..4851
                     /locus_tag="VGA92_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845418.1"
                     /translation="MNFDKKRRGISTVIGSVFFMVLMTAGLSISYLVIETQSDMIQAQ
                     QIIADGEIKKIQEKFYASASTDTGNRLAVYVENEGSNSLEIDTLWIVNKTNPTQAAQK
                     YELSHADAILAPGYGGDILQNNPLFMNPGTYDVKVVSSLGTIKTADHLNVGGPNKLKA
                     KLMMNPPDVRLGENATALLFVTNTGNTKILNVTSGPIIVTPISAVLDVSPIVQMKSDL
                     APAESTVLSWSYRLMGPAGTNVTFSTFAQGIEQPTNATIQSNTDQSLILLRDNSEPGS
                     IVSSDLFSRPEIYMIIPNTFGDANDKGLWGVNVVNPTDQPIYVSKVVISAQTSPANSN
                     DEIFDGGCANITVPPTTNDWSCPTANQLMWKNTGTPQLINPKSVFPFLVKVKPGDLGG
                     SGAEPETVIVQTNVFTTLGQFGKSAYGTSMRTSSSIPNVYLSKEVDSMSSTNIVTNMT
                     GILPGSTQTFNVVLADFDTGAASINAGSRLIINIPRGWDNVSVVGNTGFAAPTFQSFP
                     DGSSQLVGTLLAPLSGAAKTISFTATAPPITDTRMYVMYILADGTTGSGYTVGPLAEV
                     VLRVSPS"
     gene            complement(4883..5215)
                     /locus_tag="VGA92_03030"
     CDS             complement(4883..5215)
                     /locus_tag="VGA92_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008296981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEX9845419.1"
                     /translation="MVEIIVTTLGLFFFAALLEIGGGYLVWKWLRDHKGKVFGLIGGL
                     ILFSYGIIPTLQPASFGRVYATYGGIFVISSILWGYWVDKKKPDKFEILGSIIVLVGV
                     AVMFYFPR"
     gene            complement(5200..5568)
                     /locus_tag="VGA92_03035"
     CDS             complement(5200..5568)
                     /locus_tag="VGA92_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEX9845420.1"
                     /translation="MSEKAKGFASSILLFFLAGLCEIGGGYLVWLWLREDFGWIFGAL
                     GGFVLFLYGIVPVFQKANFHRIYAAYGGVFIVMSVVWGWLIDGVTPDRYDIIGTVIAV
                     IGVVIIFYIPRKGERVWSKS"
     gene            complement(5636..8140)
                     /locus_tag="VGA92_03040"
     CDS             complement(5636..8140)
                     /locus_tag="VGA92_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845421.1"
                     /translation="MLRELCLKTLLIALFACVLVLGNIIIVFAQSETDTNLMIKLSPS
                     IIEAGEKAHKIGYVGFVNPNGNLIKQTKDVIVKLESSNPEIATVPSQIMIPAGSEFSI
                     FEVTTAKSGTAKILASFGEKIKFDTLTVGQSINIGNDLRLIINLPTSEMNVNSQMPFS
                     LYLHDSNGDIAQAPFDIDIVLDYEDSLVGLETNELKISKGNSYVWSIIKSKDKVGNAF
                     LRATSDKLGFDEAKEIRISSSLPSSLAVNIFPETVPATLKRDLDVIVTLKDSDGLPTL
                     AQEDIKLQFFADDESITRQIDRLIKESQINGIIKKGEFSYRLKLKLDLPNEDKTHTIG
                     ATTKGLGVASDTFETVEPLTTNNPQAANKTLQIFTLNQIPTKSQALAIIQIGALIDDD
                     ETSDSEDESEGEFHPVIVNENYMSSGSEQKINLISSNDLLVKISNIGNIDVTSTYGTA
                     IIETGQETGQVVLSTTIKGIGSASTVTEVINTLKHERTMIFSPTGSIAMLFDKNGYFD
                     LFIISLDTKNRPTIVENENRYLLAPINSIVSIEKNKTFSHLLFQGNAIQEQDKMITIS
                     AIPIGESADVGLEAKNDFEIKPTAQLRTYVPFETLNSENVRYTGIIQVLDFYDNPIIR
                     TSDLRVKISPSEFDKVDIPEYVTIPAGASYAAFPMETNGDTSAISFVASSKGLVSGSK
                     EVDVKSQITNLKISIGSVIEPIPAEQPTVLTVYVDDEYQNAVDGVTLRVLSDDSSVSS
                     QTVRTNADGSATIQFSPKQAPKMSLQILASAEGYSEEQESFEFAVSGVVEENKTILPE
                     WMIYGGIGGIAAIVGGVIYFLKKPKKQLEDEDELYE"
     gene            complement(8171..8794)
                     /locus_tag="VGA92_03045"
     CDS             complement(8171..8794)
                     /locus_tag="VGA92_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9845422.1"
                     /translation="MNTNSDKLRLHKSRRAVAPVIATLLLVAIAVVGGAIVFAYSQNF
                     FSSSQISGRPTIEAVKILGYDARAVEIINNQNGSPFGAGSGGDIDASKEMGENIAVYV
                     KNDSVQSITVSELRFGGSIYTFASPAPDDLTNLGNGTYTIAGVAGGTGTDLITSASVA
                     EIQPGQTGSVVLSLSEDMKSGRDTQFKMTTTNGAVFVGTVVVGQQSG"
     gene            complement(8876..12799)
                     /locus_tag="VGA92_03050"
     CDS             complement(8876..12799)
                     /locus_tag="VGA92_03050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase I"
                     /protein_id="HEX9845423.1"
                     /translation="MQAQEPQIKSLSPSLLVSATYDNQKQAAVLKFYNPDSQKIILWA
                     DETGHKPYCYSKLNPDELGFLEGRKDIIKIEQIKRKDLLRDKEILVSKIIVTDPLAIG
                     GTQTEKSIRNIIETWESDIKYYENYLYDRSLIVGRFYKVQDGKIVPHNFEVTEEVSLA
                     MKSLLLDVNTTKGIVNTKEFQDYITQWAELLNQPIPKIRRISFDIEVDAEIGRIPDAK
                     IAERRVTAVGFDSSDGLKQIFILKKPGSADGKNELPPDVKMVTYDESDEKKMIEDTFK
                     LISEYPFVITFNGDDFDMPYLYNRAERLGIKNSENPLYMMRDSATLKHGVHIDLYRTM
                     SNRSFQIYAFSHKYTDFSLNSVSKALLSESKLDFGVEFDKLTNYQLAAYCYNDARLTY
                     KLTSFNNDLLMNLLVVISRIARMPIDDIARMGVSQWIRSLLYYEHRQRNALIPRREEL
                     DARSQGVVNEAIIKDKKYRGGLVIDPVEGIHFDVTVMDFASLYPSIIKVRNISYETLR
                     CSHEECKKNTIPDTNHWVCTKYNGLTSMLIGSLRDLRVNYYKSLAKKAKTLEQKELYT
                     VVSQALKVILNASYGVMGAEIFPLYFLPAAEATTAVGRHIIMETIKKCQENGIQVLYS
                     DTDSLFVKNPTSEQINKVIDGAKRDHGVELEVDKEYRYVVLSNRKKNYLGVTKDGKVD
                     VKGLTGKKSVDGKTPILANVNNVIKFSTVEQIYHEFHDNNSNVKLLTITNGLQGVWSP
                     ISDATKHMVKDVYKITTHNGRVLTLSGDHSVYTINEYGQLNCKETKNLNANNVLVGLK
                     TIPSIHNIKSINTSEYVGDITIGRNSYLISTIKHSTTGFPIKKKIPITPEVGFLLGIY
                     TSEEGYYTRTAEISQNCKFNKEVCEQIEKSWIAVFNAKMKKYGKKNKSSSYRMPFLIS
                     KLFSRACGEKSHNKKIPDLIFNAADEVKISYLRGLFSGDGFVDKKQVSLTSKSKMLIE
                     QTAYLLACFNISSNIKISTHKKYGKYYTLRVKSVQDRILFHNTIGFIQPRFRIKTTKT
                     PYNKELLPISTNGIIQIKKSILKRLGITRFRNLNMHDLRYYNMSILERYNKVIDQLDT
                     YATTNEKIALANISRMLNSNDVTYDKILKVEKMSGSQIMYDFSVPKYERFVAGNLPTL
                     LHNSHTPPFIRSLFYELLDILSKVESEKDFEEAKKKISEKIAICAVKVKEKKIPIAEL
                     AFNVMISKAPSEYNKTVPQHIRAAKLLEQHREIKRGDIISYVKIINKPGVKPVEMARP
                     DEIDSAKYMEFMESTLDQITSSMDLDFDSIIGKPKQTGLDQFFWN"
     gene            complement(12806..13327)
                     /locus_tag="VGA92_03055"
     CDS             complement(12806..13327)
                     /locus_tag="VGA92_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX9845424.1"
                     /translation="MKANFERPSWDEYFMLQAELAKLRSNCMTRQVGAVIVRNNRQIA
                     TGYNGTPPGVKNCFEGGCKRCQLRMEGKIASGESLDRCLCNHAEANAIMHCAIMGIEA
                     GTKDAILYTTFVPCLECTKMAITIGIKRIVCLDTYPETDYDLIKEAGIDVVTLDKKRI
                     QYWASTLLSEQKR"
     gene            13451..14947
                     /locus_tag="VGA92_03060"
     CDS             13451..14947
                     /locus_tag="VGA92_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9845425.1"
                     /translation="MADYIQHKFIKPNTIEAREYQINLANQAKTENCLVVLPTGLGKT
                     AVALQVIADHLSKGENGVLFLAPTRVLANQHYEFLKNNMNLEDIALLTGEDTLAKRKK
                     LWANSVICATPEITKNDLDRKIITPEQFGLVIFDEAHRTAGDYAYSSIAKRLAATSAR
                     IMGMTATLPSEKEKATEIITTLRIASVAQRNEESPDVKPYIQQTNTEWVKVELPREMK
                     EIQYYLRKALEERYGELQRCGLRLGTNSSLSQLLRARDFVLRQNRRAAKPLFTAIRLH
                     YALNILESHGVTSFLRFCERTKEKKGVGIKDLFENDSNFLRALAHAKDAQKKGIEHSK
                     IVKLAEIIKSIQGKAIVFTSYRDSVEIIHSKLVEMGVSAAFLIGKAGETGLKQKEQID
                     TVQKFRDGIYNVLVATRVGEEGLDISEVNDVIFFDNVPSSIRFVQRKGRTGRKSIGKL
                     TVLIAKDTIDEAYYWVGQRKIKAAQGMGDKLTKDLQKQQTKSVLDAYF"
     gene            complement(15163..15441)
                     /locus_tag="VGA92_03065"
     CDS             complement(15163..15441)
                     /locus_tag="VGA92_03065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9845426.1"
                     /translation="MSDKIKCAHILVEKQSQALAILERLKNGEKFAEIAKQLSLDPGS
                     GKRGGDLGYFGRGKMVKPFEAAAFKLSVGQLSEPIKTEYGYHIIKRLA"
     gene            complement(15475..15867)
                     /locus_tag="VGA92_03070"
     CDS             complement(15475..15867)
                     /locus_tag="VGA92_03070"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9845427.1"
                     /translation="MKFTEGEKKFLGSLEEARLATANDSMPHVKPVSFIFLDDVFYVA
                     TDYSTRTFKNVKKNPRAAISVDIYKPGGHKAVLAQGKIEIVEKGLKFKKIYEKFFEKF
                     EWVRRDPWKEGEAPFLKLIPTKKTSWGT"
     gene            complement(15864..16148)
                     /locus_tag="VGA92_03075"
     CDS             complement(15864..16148)
                     /locus_tag="VGA92_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845428.1"
                     /translation="MSEDEQIEQLIIQTLDGALQSIPAYLEEIEENKAELKVGNVKEF
                     VYGTVMGMALGMASAAIAALRGGMPTEADQQKIRDMVYSKIPQVRERIFG"
     gene            16234..16635
                     /locus_tag="VGA92_03080"
     CDS             16234..16635
                     /locus_tag="VGA92_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX9845429.1"
                     /translation="MPSYFTIAAANEALPKVIEKYKNLQKQKQEVQKLEAQLNTQMSV
                     SFNLADYVAIKQQLNAAVTKFYQLVEDLENTGVVLKSLEEGLLDFPSKRFDEEVWLCW
                     KEGEKEIKFWHEKDVGFIGRKPLSVSDESLV"
     gene            16632..17534
                     /locus_tag="VGA92_03085"
     CDS             16632..17534
                     /locus_tag="VGA92_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX9845430.1"
                     /translation="MSLSTWFRIIRVKFLLASVIAVSLGLAVSYWQTQRLDVAHSIIT
                     MAGVLALHASVDLLNDFWDYKRGIDTTTKRTKFSGGTGVLPEGLLESSHVYRAGVIFL
                     ILGSLAGIYFIVLYGWIIAAILGFAVLSIYFYSTKIVDSGLGEIFVGIKGTMIVLGTI
                     FIQTQGIEPANIVAGVIAGALSSFVLYITSFPDYEADKQKGRRTLVIVLGKNKASVLF
                     WVFPAIVYGLIVYGVATEILPVFCLVAFATIPLVGIGTKIKKTLDDAERFVSIMKTTL
                     VFSRITGLLLIVGFLIEIIKNTYS"
     gene            17560..18660
                     /locus_tag="VGA92_03090"
     CDS             17560..18660
                     /locus_tag="VGA92_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9845431.1"
                     /translation="MLSGFATPNGTKKFAEKFGQLQDNYKTFQDLTLSNVGIGTYLGD
                     QDDQTDLLVTAAVKKSVLSGINVIDTAINYRAQKAERSVGKAVSELVSEGKIERDQVF
                     ISTKNGYVTNDAAIKEEFWSYVQREYAKPGIIGPNDISSGYHCMTIKYLEDQLGRSLK
                     NLGLECVDLLYLHNAIEGQIQDISKEQFIKNLKDVFGMYEQKRKEGKIRYYGMATWEC
                     FRVQKENPQHLSLSDVIRLAKEAGGEDHGFRFIQLPYNLYYDQALMLKNQPISGDEVS
                     ILGAAQNYGVGVFTSVPLMQGRLLAPGALPDFDGLKPSLRCLQFIRSTPGVLAPLVGQ
                     KNPSHVDENLEIMKIPPMKKDEFEGLVKKLVS"
     gene            complement(18657..19355)
                     /locus_tag="VGA92_03095"
     CDS             complement(18657..19355)
                     /locus_tag="VGA92_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845432.1"
                     /translation="MDRLAKKHRGLTFWYNAALITFAAVAIGLTWTVMSSGFATSEAV
                     KETVEDAVVSADTLQIIGKMAGTAEVSNNKVTATATPITATTSGVVDMSAEDVRVTYQ
                     LIKDGSYTITHDDIYAGSLYGESHNSLNSALIAAKEKGLIAINPQADQQKPDTTTAFL
                     YWVINQDFDQNVQNNEIASLVVVYADKDRPSTGEYIRIQVVENDGVILDIERTVPDIS
                     SSILDFGGKVKDEN"
     gene            complement(19450..19599)
                     /locus_tag="VGA92_03100"
     CDS             complement(19450..19599)
                     /locus_tag="VGA92_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845433.1"
                     /translation="MLEPGKIKEGLDNNTSPYLMSCKAETGWVGQITVGTTGIHACRD
                     QKLGR"
     gene            19719..20672
                     /gene="dusB"
                     /locus_tag="VGA92_03105"
     CDS             19719..20672
                     /gene="dusB"
                     /locus_tag="VGA92_03105"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX9845434.1"
                     /translation="MSELPKFPSRAFLAPMAGISDVALRLLCREMGAGLVVTELTSIH
                     AIIAKEKQLTLHNKQISEFIEFSEKERPISVQLFGSDLDALQRAAKIVEPFFDMIDYN
                     MGCPAPHITEQMAGGALLQNIDLTRKIFRTLTASVKKPVTLKMRAGVENTGLFKEIAM
                     VAQQEGIKMITLHARTVKQGYSGMSNWDLIRDLKELVDIPVVGNGDITNPELAKQMMQ
                     QTNCDYVMVGRGAMGNPFLFKQINDYLSSGSYQHYQLKTRLEIFNKYIEYAIHYKIRF
                     SSIKQQAMRFTKGIADGTKLRPKIMLANTIDELNTIMVAAA"
     gene            20731..20877
                     /locus_tag="VGA92_03110"
     CDS             20731..20877
                     /locus_tag="VGA92_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX9845435.1"
                     /translation="MPVDPVCGIEMDESLAVTFEFDSKKYFFCCNGCRSIFKKKPKKW
                     AKKT"
     gene            complement(20874..21821)
                     /locus_tag="VGA92_03115"
     CDS             complement(20874..21821)
                     /locus_tag="VGA92_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX9845436.1"
                     /translation="MQEIIDSAYEILEKAASKCTAEHLLLSGGLDSTILAYFLKNRKT
                     SCITIISKDFVANDLTYCQLAAQRFGLPLHIKMCDAAEIYNGVEETIKILKNFNDIEI
                     RNNVVIYLALSELKKLGFDKIITGDGADEIFAGYNFLLNKSKDDLERDLQRISKIMHF
                     PSQKIGKSLGVVVESPFCTKEVIDFAKTIPVDLMVRQENEKKFGKWILRKAFEDKIPR
                     SIAWRSKSPMQDGAGTQGLTGLFEVTIPDNVFAEKAKKIKENDGITIRTKESLQYYEI
                     YRKYCDMPVFDEAGTKCPDCHHMIDEDSKFCRMCGRFPL"
     gene            21966..22382
                     /locus_tag="VGA92_03120"
     CDS             21966..22382
                     /locus_tag="VGA92_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9845437.1"
                     /translation="MLLPAEIESKTLIPALRAILAKKLAEEHNVREDEISKMLGVTQA
                     AISNYIRGTRGDPKLIQKLVTDEQVSQLINDLSDRLASDMAYTPSSLAKFISLCNYIK
                     SSLLICEIHHNLESNIDEAICKECENMLLKGPGSVY"
     gene            complement(22384..22692)
                     /locus_tag="VGA92_03125"
     CDS             complement(22384..22692)
                     /locus_tag="VGA92_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9845438.1"
                     /translation="MISMEGIQNPDQVSPKNEPYVYYVRDEPLMQAALYVFTLINKHG
                     KSILLAKGNSIPSAVAIANIIVEKMLNRVCTVEHITLDSENIYGKKMLSSIEISLVRK
                     "
     gene            complement(22744..23244)
                     /locus_tag="VGA92_03130"
     CDS             complement(22744..23244)
                     /locus_tag="VGA92_03130"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HEX9845439.1"
                     /translation="MSEPEEAEVEVVEEVEEEPEFVRPVVEDGSEDTSMEDAINAYKK
                     IFETKEITDDERAELDKKIKAMEQREIIDVDPIHVIVEIPLAGKGKIAIGPPVLTRFE
                     KARILGARALQLSQGAPPFIQIPATARTSLDIALQELEDRAIPIVIRRRLPNGDYQNI
                     PIDYFN"
     gene            23296..23700
                     /locus_tag="VGA92_03135"
     CDS             23296..23700
                     /locus_tag="VGA92_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEX9845440.1"
                     /translation="MSAGSVSRSHLIVEVKGKSRMTCELKRHLSPKTVGSILRSLPLE
                     GNAHFLGQNIVYFETAVNSGVERQRKEFKKGDIAFSPAGGSICFFISDVVSSKAMTPI
                     GKLLSDVDAISAVKPGDVIAVYQQSADISSGH"
     gene            complement(23654..24010)
                     /locus_tag="VGA92_03140"
     CDS             complement(23654..24010)
                     /locus_tag="VGA92_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX9845441.1"
                     /translation="MDKISTKIMGGTKKKSAAQMEKSQDTDKTKKDSQGKKDKKGEKT
                     ERRAEITVLVNEDQALKLIKSNNYLTVQELAKQTGVKISAANACLVNLVKKGSVKRAG
                     GFSGHWIYQQIADKPQ"
     gene            24135..24515
                     /locus_tag="VGA92_03145"
     CDS             24135..24515
                     /locus_tag="VGA92_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845442.1"
                     /translation="MTRRYLDKKTKDRFKRLVRHDDGSENKKYITESGPKVPEFEEAV
                     QAKRMIFLRDLGGDAQSADEILNEKIGELLNTAQETSDYIKNVNTHLDKQKIRVDELK
                     KLQKMFESQVSALETKDKQKSNSD"
     gene            complement(24564..24944)
                     /locus_tag="VGA92_03150"
     CDS             complement(24564..24944)
                     /locus_tag="VGA92_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845443.1"
                     /translation="MSTTSWQPSILIVDDSPFSRRAIRNIIEQNRLSNIIIEAGDGVE
                     AVMKYKEHKPTLVTMDVLMPKADGIQALRAIKKLDPNAKVIMISSTGKSHIVQDAMRA
                     GAVDYILKPFEQSQIAIALSKHSR"
     gene            complement(24941..25834)
                     /locus_tag="VGA92_03155"
     CDS             complement(24941..25834)
                     /locus_tag="VGA92_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845444.1"
                     /translation="MSHSEYANVNSKIVKTIHDIKNTGIIGVQINSTLHNIILNLVSN
                     KISKIFVFDNDRPVGIITDKDIIRFLYIDKSGRSLDSVYAAEIMNSVCFVFDNMTCQQ
                     AAQMMIINKISTLAIGSKENLEGIVTKTDLLKYYVNNQQDKSKVSDYMTVSYFSARTD
                     DRLYEIIKKMILYDISRIVVTDSANNPVGIITNGDIFKITMTTSNLDIVQSSLSRYTE
                     QDGLWSETGFVGSQLAAETMTEGIITIDSDTNVKTAAKILVDKRIDCLGINNSQGQLV
                     GLINKTNILYALANSNRECPQ"
     gene            25993..26205
                     /locus_tag="VGA92_03160"
     CDS             25993..26205
                     /locus_tag="VGA92_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845445.1"
                     /translation="MGIVAKGAKCDVEGCDKDGTRSLGSSRVEAAGLRLKSSGKKAVL
                     CKEHYKEWKKETKEDRELERARYDRF"
     gene            complement(26202..27287)
                     /gene="speY"
                     /locus_tag="VGA92_03165"
     CDS             complement(26202..27287)
                     /gene="speY"
                     /locus_tag="VGA92_03165"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX9845446.1"
                     /translation="MDHHHFHGKNIPHIRLNQKTTISELVDVFAGSGYNGRQLGDAAK
                     LYAQMIKEGATICLTVSGAMTPVGFGGIIKTLIEKGFVDWIITTGANVYHEDHFAWGL
                     PVKQGHFEVDDMVLYEKEIVRIRDVYIKFCETLEAEDQIIQKMFKNQLTDAPFTTAEF
                     CNFMGKVSSQKAKHPDKSFLVSAYKYDVPVYVSTLKDSSLALNLGIHRLHNKVYSLDF
                     VREVLEQAAIVYNSKKSGILELGGGVPKNTAQQTGPMLDQILRRHDGGQDYIIQITDA
                     RPDTGGLSGATLQEGKSWGKVQDSHEGLVTVYTDSTIAFPIIAAYVLATQKPRKPKRI
                     YKNLNKFYTKLQTEFLKNAKTNNYEQD"
     gene            27371..27700
                     /locus_tag="VGA92_03170"
     CDS             27371..27700
                     /locus_tag="VGA92_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845447.1"
                     /translation="MKIIDVANPERINRKPDRTIMLLSSGNLAEQGYVIKKIELRLFV
                     EKIDQKLGPFSLITSLVETDKGEVEMMYDEGYRGEDVLEKTANFIVNNLGLSGMILRS
                     LLVLQSK"
     gene            27756..28124
                     /locus_tag="VGA92_03175"
     CDS             27756..28124
                     /locus_tag="VGA92_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845448.1"
                     /translation="MSLRILIADDEKDNADSYAEILRQRGHQVVVTFSGVDCITEYLS
                     AFDYEQNSPYDVVIIDYSMPDMSGVQAARKMLDMNPKQQIIFISGYGSELLSHLDAFH
                     NVDLLTKPIIPNALIRLVED"
     gene            28215..30059
                     /locus_tag="VGA92_03180"
     CDS             28215..30059
                     /locus_tag="VGA92_03180"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9845449.1"
                     /translation="MRILQLHCDSIEFTPTKKEIKSAEEIEPATKRFEEIVVAFVAIE
                     DGDDSEIAKKAIAEIKSSMEKVGCNKLLLYPYAHLSSNLASPGTALNLLKEMESLANC
                     EVSHAPFGWTKSYNIKVKGHPLAESSKTITKGEHEHTSEALKSESKIQSFWHILTPDG
                     KLHDVGTFDFSKHKKLEVLAKYETAKKRAVDEPPPHVKLMKKMAIADYEPASDSGNMR
                     FYPNGRLIKSLLERFVTDEVKNYGGLEVETPIMYDSHHPSMESYFNRFPARQYNINAE
                     GKQLFLRFAACFGQFLMANDFQISYKNLPLKLYELTRYSFRREQSGELVGLRRLRAFT
                     MPDCHAFCADMPQAIEEIRKRFDLSRLVLRGVGIAESDYEMAIRFTEEFYNENKSIIE
                     ELVKKIGKPVLVEMWKERFFYFVLKWEFNYVDNLGKASALSTDQIDVENGERYGIKYY
                     DENNVPHHPIILHNSPSGAIERLIYALLENAAFDQKQGKKPHLPLWLAPTQVRIIPLK
                     EEFLKYCEDLADKISQSEIRVDIDDRNETIGKRIRESETDWIRYTLVIGEKEINQSNL
                     SVRDRSSGDVKELSFDGFIKEIKEQTKDKPFSKLNLAKYLSQRPQIMV"
     gene            30126..30548
                     /locus_tag="VGA92_03185"
     CDS             30126..30548
                     /locus_tag="VGA92_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845450.1"
                     /translation="MGLNILVAEDSVAYAVLYQQIFESRGHTVKLTLDGEECMSAYAQ
                     EAAKKQDSQNPFDVVILDHSMPKKTGFDVAKEILETKPNQKIMIITGFDDEISAKLKQ
                     SDMGKNIEVIEKPFNSSVLINKIERETMKEKASHVLIS"
     gene            30607..31473
                     /gene="speB"
                     /locus_tag="VGA92_03190"
     CDS             30607..31473
                     /gene="speB"
                     /locus_tag="VGA92_03190"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX9845451.1"
                     /translation="MSYLDLYMNKAPLIIPPNEEGEPVAIVFGIPFDSTHSYKPGTRF
                     GPDAIRDAFNNIEIFHPQLGVDLESVNIEDLGNTKHTVNVSEMTDMVNKITGELAKKN
                     KLLVILGGEHSLTYGTYTAFPKDTGYIVFDAHYDLRDGYADVHLSHAAYLRRVVEKHG
                     AENIIHVGARSFVKEELAFLKEHNIKTISDKEIRNGDGPKLVKDALSNFSKTYVSIDL
                     DVLDPAFAPGVGNPEAVGITSREMFDMVYAMGGSKISCVDIVELNPTYDNGATASLAA
                     KLMSTIIAMSIK"
     gene            31496..32074
                     /gene="pgsA"
                     /locus_tag="VGA92_03195"
     CDS             31496..32074
                     /gene="pgsA"
                     /locus_tag="VGA92_03195"
                     /EC_number="2.7.8.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylinositol phosphate synthase"
                     /protein_id="HEX9845452.1"
                     /translation="MLNNFRDSLKPHLEKLGNEFASTGFSPNFWTAVGLAFAFLSAIA
                     YGLNGQIQHVAIIGGILLLISGFFDVVDGQVARVTKKTSKKGGFLDSVFDKIAEVAIF
                     LGILVGNFAEPFLVFLAITLSLLVSYTRSRAESLGVKLQGIGIGERAERLLVIAIIGM
                     VGFMQYAVIIVIIIAAITFIQRIIVTAKNIPE"
     gene            complement(32076..32363)
                     /locus_tag="VGA92_03200"
     CDS             complement(32076..32363)
                     /locus_tag="VGA92_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S26e"
                     /protein_id="HEX9845453.1"
                     /translation="MPLKRASRGRRKGGKGSSDRIQCTNCGATVPKDKAKKVTSRLNL
                     VEHTLARELRAQGAYIAAPKVLKWYCISCAIHFGILKIRSADSRRQAGRLY"
     gene            complement(32386..>32587)
                     /locus_tag="VGA92_03205"
     CDS             complement(32386..>32587)
                     /locus_tag="VGA92_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845454.1"
                     /translation="IFGATVNTCSKINSLAKPNTMIVGESLYEKVKGIKGYIFEKTSS
                     YTIDVENKFDVYEVRVETAPRI"
BASE COUNT         9310 a         6730 c         6745 g         9802 t
ORIGIN      
        1 aggcgtgctt tcctcctata tatgataatt tttgaacgaa gttattcagg tcatcctcat
       61 tttcaaatac tatgtttgtt ggcactgatt cgaactctcg atgccatatg tacagtggga
      121 cgttggttcc atcgcagctg atctcctcaa tcatgtggtc gcgcatgagt ggctcaattt
      181 ttcccatgta tacaaagtcc cttatcgcat agtatgtgat ctttgagagg ctttttgtct
      241 ggatgtctat gttgtatttt tttatcaggt gaagaatttt gcttttgagc tcctttgcag
      301 cttctttttt tgtcttgatg ctgtttaatg aaatgtcaag ctctgatatc aaaagttttt
      361 ttatcacacg gaaattgacg ttgtcgcttt tgttcatctg gggctcgatt atcatgtatc
      421 gcaagccgtt tctcgatgtc gtatcagcta tgagtccagt ataaatttct gcatcctcaa
      481 agtctatttt gtttaatgtt ttgaattttg gcaccagtac attttcaatc tcgtttattg
      541 gactgtcttg ttgactggca atttttgcaa accttgtttc cgaagtattt agaatttgtt
      601 tagagtcagc atgatctgca gattcgtttt tgtttaaaac aaactttgaa aaattaaatg
      661 gattagtttt tcggatccgt ataaaataag aatcattctg ggctttgtta acattcaacg
      721 ttttgtagta ccttcaaatc tctcataaag aattgttagt gcaaatgaac agcatccgat
      781 ttacattttt gactacagaa tagaagtgaa gcgcaagatc atgactaaaa ggaatggggt
      841 gtaatgtttc agctaaatag gaatcactca aagctcaaag gtgcacagtc agcagaccaa
      901 aaaatcccca aattggctca aaaaccggca ctagttgacg atgatctgcc agaaatcaag
      961 gaactaatcc ggtggaatga ggaaaacaag caaaaaatct caaaactgct ggataaaatc
     1021 aagcaaaact tcaacggcgt gctcatacct cagattgacc ataacaaagg cgccgttcat
     1081 tatccagccc tcggtgaaat aggagagcta acaactaatc cagtttttct tgaaaaactc
     1141 tcccacagaa agttaggatt tcttgaaaaa tttgtttaca accaattctt aatctgcccg
     1201 gatcacaggg cgtctttttt gctcaatgtg aggctgtgct gcccaaagtg caactcaatt
     1261 gatgtccaca agctgcatct ctttgagcac aagatctgtg ggtgcataat ggaaaaacca
     1321 aagttcgttg atgcacaaga agcagacaag ctaaagtgtc catcctgtaa ccgggtgatt
     1381 aaaaatccgc aaaaagaact aagatctcca gcgatgtggt atttttgcaa tgactgcaaa
     1441 gaaaaatttg atgaggtatt aatcaacttt cactgcaaag agtttgatca tgattttaca
     1501 ataaatgatg cacaaacagt aactgtctgt ggatacgcgc ttaccgacgg ctcagaacac
     1561 tctccacttg actatcaaga attaaaaagt gaaatctcca gggtcttatc caagcttggc
     1621 ttttctgtag atgaaaacta taccataaaa ggtaagagcg gacacgatca caaaatcgac
     1681 atttatggaa gcaataacaa aaaccagact gcattcattt tggtaaatga ctcaaacgac
     1741 tctgagatcg attcaaaaat aattcaaatc cttgacacct caccaaaaat agcgctaatt
     1801 atcggtgctg cgtcagttaa cgagaaaaca aaagcgattg catcaaaata caacgtctcg
     1861 ataatctcgt ctcagaacat gagcgaaata gtttctgagg ctgaaaagat actcacatac
     1921 aggctccaaa agctggatgg cgtccaactt agatgaacct aaaaagatac gccacctgta
     1981 caaaaaaacg cggagtcagc caaatcattg gtacgatgtt tatgcttgca ctggtggttc
     2041 cggtgggaac ggtaattgtg accaaagggc tcaatgaggt gggcgagatg agcaatcggc
     2101 ttacaagcgg aatcacgtat caaaacgaag gaggccgtga ggacattgta tttgagcaca
     2161 taaggctaga tcctacggga aaccaagtta ccatcacgct acgaaatgct ggaaccattg
     2221 atacgacaat taacaagatt acgatggtaa aaattgacac gcaagaattg ctaataagcg
     2281 acaaaagtct tgccgtcgac gctcagccga gaattggcac cgacatacta tcaacagcga
     2341 gccttcagtt ttcaggacgc tgggatgatc caaactacgt aaattcggaa tacaagatct
     2401 caattcttac atcaaaagga aatttcttcg aaacagtggc gaggccgttt aacacttgag
     2461 acaaaaatta ttgcaagcta aaagacgcgg aatatctgag ataattgcca cgttgctgtt
     2521 gcttggaatc acaactgccg gctctgtgtt tttggcaaca gttgtgcagg gtagcgggct
     2581 tgcaagcatt cagggtagtg cggtaacccc agtctcccca ccatactcaa tacgactaac
     2641 aggttatgat actcgggatg gtgcaaacct catagatctt aacacacttg ataacaaatc
     2701 tgacaaaaaa ctttgcacaa tctcttgctc tttgacgcca agcaacatcc cggaaaatga
     2761 tggtactgat tttattgcaa tccagataaa aaatgtcagt acaaacccgg tctttatcaa
     2821 aaacatccag atcaacggcg taacacatga ctgggatgct cagacgggaa ccaaaacatt
     2881 tgatgcaagt gcggacaatc ttttaggaaa ttatcctctt gatggaaaat ttagcatagt
     2941 gtcctcaaac ggcgtgcaaa aatcagacaa caggattgta gatgacgaag aagtacgact
     3001 agttgtaaag ctaagcaagg atattgcctc tgatgttttg ctctcaaagc caatccaagt
     3061 tttagttgac tttggcggta cacgctctac tgagtttgta gtgttaagcg gtgatgcaaa
     3121 gtgactggaa tgaattttga caaaaagaga aggggaattt cgactgtaat cggctctgtg
     3181 ttttttatgg tgcttatgac tgctggcctc tcaatctcat atcttgtcat agagacgcaa
     3241 tccgatatga tacaggccca gcaaatcata gcagacggcg aaatcaagaa aatccaagaa
     3301 aaattctatg cgtcggcatc tactgatacg ggtaataggc ttgcagtgta tgtagaaaac
     3361 gaaggctcca attctttaga gatagatacg ctttggatcg taaataagac aaacccgact
     3421 caggcggcac aaaaatacga actaagtcat gctgatgcga ttctggcacc tggatacgga
     3481 ggggacatct tacaaaacaa tccgttgttc atgaatcctg gaacatatga tgtcaaagtt
     3541 gtatcctctc ttggcacaat caaaacagct gatcatctga atgtgggtgg gccaaacaag
     3601 ctcaaagcaa agcttatgat gaatccacct gatgtgcgat tgggcgagaa cgcaacggca
     3661 ttgttgtttg tgacaaatac tggaaacacc aagatactga atgttacttc tggaccaatt
     3721 attgtaacgc cgatatcagc cgtgcttgat gtatcgccaa tagttcagat gaaatcggat
     3781 ttggcaccgg cagaatctac cgtactttca tggagttata gattgatggg tcctgcggga
     3841 accaatgtca cattttctac atttgcgcaa ggaatagaac agccaacaaa cgcaacgata
     3901 caaagcaata cagaccaaag cttgatctta ttacgcgata attcggaacc aggctccata
     3961 gtttcatcgg atcttttctc aagaccagaa atatacatga tcatacccaa tacatttggt
     4021 gacgctaatg ataaaggact ttggggagta aatgttgtta atccaacaga tcagccaatt
     4081 tatgtcagta aggtagtcat tagcgcccag acttcgcctg caaacagcaa cgacgaaata
     4141 tttgatggcg gttgcgctaa cataacagtg cccccgacca caaatgattg gtcctgtcct
     4201 acagctaacc agctaatgtg gaaaaacact ggtacgccac agctaataaa tccaaaatca
     4261 gtgtttccat ttttagttaa agttaaacct ggggatttgg gtggaagtgg agctgagccg
     4321 gaaactgtta ttgttcagac gaatgttttt acaacacttg gccaatttgg taaatcggcg
     4381 tatggtacat ctatgagaac ctcttcttcg ataccaaatg tgtacctatc caaagaggtc
     4441 gactctatga gtagtactaa cattgtgaca aacatgactg gaatccttcc cggttcgact
     4501 caaacattca atgtagtttt ggcggacttt gatactgggg cagcttcgat taacgcgggt
     4561 tccagactaa taataaacat accaagggga tgggataatg tcagtgttgt gggaaataca
     4621 ggttttgctg ctccaacatt tcaatcattc ccggacggtt cttcacaact agtaggaaca
     4681 ctacttgcgc ctctgagtgg ggcagctaaa accataagct ttactgcaac tgctccacca
     4741 ataacagaca ccagaatgta tgtcatgtat attcttgcag atggaactac aggaagtggt
     4801 tatacagttg gcccattggc agaagttgtt ctacgtgtct cgccaagcta atttttttat
     4861 tattgaaaaa taaaaaaagg cttcatcgtg ggaaataaaa cattacagct acgccgacaa
     4921 gaacgattat cgaaccaaga atctcaaact tgtctggctt tttcttgtct acccagtacc
     4981 cccaaagaat tgatgatatg acgaatatcc caccataagt agcatacacc cgtccaaagc
     5041 ttgcaggctg aagcgtcggt attatcccat atgaaaacaa aatcagtccg ccaattaatc
     5101 caaaaacctt tcctttgtga tcccgcagcc acttccatac taggtatcct cctcctattt
     5161 ccagcagggc tgcaaaaaag aacagcccaa gtgttgttac tatgatttcg accaaaccct
     5221 ctcacctttt cttgggatgt agaaaattat gacaacgcca attactgcaa tgactgtccc
     5281 aatgatgtca tatctatctg gagttacacc atcaattaac cacccccaaa caactgacat
     5341 cacaataaag acgccaccat acgcggcata gattctatga aagttggcct tttgaaacac
     5401 cggaactata ccatacaaaa acagaacaaa accgcctagg gcaccaaaaa tccacccaaa
     5461 gtcctccctt agccatagcc acaccagata accgccgcca atctcgcaaa ggcctgccaa
     5521 aaagaaaagc agtattgatg atgcaaaacc cttggccttt tcactcatac cagtgtagcg
     5581 ttaagacgct aaaattatag ttattacggc aatgctatgg tgtgtctggt gggatttact
     5641 cgtaaagttc gtcttcgtct tctagctgtt ttttgggttt ctttagaaag taaatcacgc
     5701 cgccaacaat tgctgcaatg ccgccaatgc caccataaat catccattct ggcaagatgg
     5761 ttttgttttc ctcaactaca ccacttacgg caaactcaaa tgattcctgt tcttcgctgt
     5821 atccttcagc tgaggccaaa atctgcaaag acatctttgg cgcctgcttt ggactgaact
     5881 gtattgttgc actgccatcg gcatttgtcc tcactgtttg cgagctgacg gacgaatcat
     5941 cagataatac acgcaaagta acaccgtcaa cggcattttg atattcatca tcaacgtata
     6001 ctgtcagtac tgttggctgt tcggcaggaa tcggttcaat tacacttcca atgcttatct
     6061 ttaggtttgt gatttgtgat tttacatcta cttctttaga accgcttacc aatccctttg
     6121 agctggctac aaacgatatt gcgcttgtgt ctccatttgt ttccattggg aatgcggcat
     6181 acgaggcccc agctggtatt gtaacatatt ctggtatgtc tactttgtca aactctgatg
     6241 gagaaatctt tactctcaag tcactagtcc gaattattgg gttgtcataa aagtccagga
     6301 cttggattat tcctgtatat ctgacatttt ctgaattcag agtttcaaac ggaacatagg
     6361 ttcgtaattg agcagtgggc tttatctcaa aatcattttt ggcctcaaga cctacatctg
     6421 ctgattctcc tatcgggatt gcactgattg tgatcatctt gtcttgttct tggatggcgt
     6481 ttccctgaaa gagaaggtgg gagaaggtct tgtttttctc aattgagacg atgctattga
     6541 tgggggccag taggtacctg ttttcgtttt caactattgt gggccgattc tttgtatcta
     6601 aggaaattat gaacaagtca aagtagccgt ttttgtcaaa tagcatggca atgcttcccg
     6661 tgggagaaaa tatcatggtc ctttcatgct tgagcgtatt gattacctca gttactgtag
     6721 atgcagaccc tatgcccttg attgtggtgg ataaaaccac ctggccagtt tcctggccag
     6781 tctcaattat tgccgtacca tatgttgatg tgacgtcgat atttccaatg ttgcttattt
     6841 tcaccaaaag gtcgttgctg gaaatcagat tgatcttttg ctcgctccct gacgacatat
     6901 aattttcgtt tactataacg ggatggaatt cgccttctga ctcgtcttca gaatcagatg
     6961 tctcgtcgtc atcaattagt gcgcctatct ggattattgc aagtgcctgt gactttgttg
     7021 ggatctggtt taaagtaaat atctgtaatg tcttattggc agcttgcggg ttgtttgttg
     7081 taagcggctc cactgtctca aatgtgtctg aggccacacc aagacccttc gttgttgcgc
     7141 caatggtgtg tgttttgtcc tcattgggga ggtcaagctt tagctttagt ctatagctaa
     7201 actcgccttt ctttattatt ccattaatct gggattcttt gatgagtctg tctatctgac
     7261 gggtaatgga ctcgtcatct gcaaaaaatt gtaatttaat gtcttcttgt gcaagtgtgg
     7321 gcaagccatc tgagtctttt agcgtcacga ttacatcaag atctcttttg agggtggcag
     7381 gaactgtttc tgggaatatg ttgactgcaa gagaagatgg cagtgacgag gatatgcgta
     7441 tttcttttgc ctcatcaaat cctagtttgt ctgatgtggc cctcaaaaat gcattaccga
     7501 ctttgtcctt tgattttatg atggaccaga catagctgtt gcccttgctt attttaagct
     7561 cgtttgtttc taatcccact agactgtctt cgtaatctaa aactatgtcg atatcaaatg
     7621 gtgcttgtgc gatatcgcca tttgagtcgt gcaggtataa tgaaaatggc atctgcgagt
     7681 ttacgttcat ctcacttgtt ggcaggttta tgatgagcct cagatcattg cctatgttga
     7741 tgctttggcc aactgtgagt gtgtcaaatt ttattttttc tccaaatgag gcaagtatct
     7801 ttgccgtgcc gctttttgca gtggttacct caaagataga aaattctgag cctgcaggta
     7861 tcataatctg tgatggaacg gtggcaatct ctgggttgct tgattctagc ttgactatga
     7921 catcctttgt ctgctttatc agatttccat ttggatttac aaaaccgaca tagccgattt
     7981 tgtgcgcttt ttctcctgcc tctatgattg aggggctgag ttttatcatc aggtttgtat
     8041 cagtttcaga ttgtgcaaaa acgattatga tgtttcctaa aactaggacg cacgcaaata
     8101 acgctatcaa aagtgttttc agacatagtt cgcggagcat tttggcctcc ataaaaaaga
     8161 aaagaggggg ttatccactc tgttgaccta caacaactgt gccaacaaat actgcaccgt
     8221 ttgtagtggt cattttgaat tgggtgtccc tacctgactt catgtcttca ctgagtgata
     8281 gtactacact gcctgtttgg cctggttgga tttctgcaac agaagctgag gtaatgagat
     8341 ctgttccagt tcctcctgca actcctgcaa tggtgtaagt accgttcccg agatttgtca
     8401 aatcatctgg tgctgggctg gcaaatgtgt atattgatcc gccaaatctg agttcgctta
     8461 cagttattga ctgtacacta tcgtttttga cgtatactgc aatgttttct cccatttcct
     8521 ttgatgcgtc aatatcacca ccagaacctg ctccaaatgg agaaccgttt tggttgttga
     8581 taatttcaac tgcccttgca tcatatccta gaatctttac tgcctcgatt gtgggtcttc
     8641 cactaatctg tgaagagcta aagaagttct gtgagtatgc aaagacgatt gctccgccta
     8701 ctactgctat tgcaactagc aacagagtag ctatgactgg cgctacagct cggcgtgatt
     8761 tgtgaagtcg tagtttgtct gagtttgtat tcattgatga attgtagcca ttagatgctg
     8821 ttaagacgga tgctgtgcat caaacacaac atttttccat ataaaaacgc aaaaactagt
     8881 tccaaaaaaa ctggtcaaga ccagtctgct ttggcttgcc aattatagag tcaaaatcca
     8941 agtccatcga agacgttatc tggtctagtg tgctttccat gaattccatg tattttgcag
     9001 agtcaatttc gtctggccgg gccatttcca ctggcttgac gcctggcttg tttatgattt
     9061 tgacataaga tatgatgtcg ccgcgcttta tttcgcggtg ctgctctagc agctttgcgg
     9121 cacgaatgtg ctgcggaacg gtcttgttgt attctgatgg ggccttgcta atcataacat
     9181 tgaaggcaag ctcagcaatt ggaatctttt tttctttgac ctttactgca caaatggcaa
     9241 ttttttcgct gatcttcttt tttgcctctt cgaaatcttt ttctgattct acctttgata
     9301 aaatatcgag caattcataa aacaggcttc tgataaatgg cggggtatga ctgttgtgta
     9361 ataatgttgg caaatttcca gcaacaaaac gttcgtattt aggcaccgag aaatcataca
     9421 ttatttgact gcccgacatc ttttcaacct ttaatatttt atcatatgtg acatcgttgg
     9481 aatttagcat tcttgaaatg ttagctaatg ctattttttc atttgttgtt gcatacgtgt
     9541 ctaattgatc aattacttta ttgtatctct ccaaaattga catattataa taccgtaaat
     9601 catgcatatt caaatttcta aatctcgtaa tacctaacct tttaagaatt gattttttta
     9661 tctgaattat tccatttgtt gatattggta gcaattcctt gttatatggt gtcttagttg
     9721 ttttaattct gaatctgggt tggatgaatc ctatagtatt gtgaaacaat attctatctt
     9781 ggacactttt aacacgaagt gtataatatt ttccatattt tttatgagtc gatattttta
     9841 tgttagatga aatgttaaag catgccaaca aatatgctgt ttgttctatc aacattttac
     9901 ttttagatgt aagagaaacc tgttttttat ctacgaatcc atctccagaa aaaagaccac
     9961 gcaagtaaga tatctttact tcatcagctg cattaaaaat taaatctgga atttttttat
    10021 tatgactttt ttctccgcat gctctactaa acaattttga tatcaaaaat ggcattcggt
    10081 acgaactcga tttatttttt ttaccatatt ttttcatttt tgcattaaat acggctatcc
    10141 atgatttttc tatctgctca caaacttctt tattaaattt acaattttgt gatatctcgg
    10201 cagttcttgt atagtaacct tcctcagatg tgtaaatgcc taacaagaat ccaacctctg
    10261 gcgtgattgg gatttttttc tttataggaa atccagtagt cgaatgttta atggtggaaa
    10321 ttagataact atttcttcct atggttatgt cgccgacata ttctgaagtg tttatggatt
    10381 ttatgttgtg tattgatggt attgttttca atccaactaa cacattgttt gcattcaagt
    10441 ttttggtttc tttgcaattc agctgaccat attcattaat cgtgtataca ctgtgatctc
    10501 ctgaaagagt taaaactcta ccattatgtg tggttatctt atacacatct ttgaccatgt
    10561 gtttggttgc atcagaaata ggagaccaaa caccttgcaa tccgttggtg attgttagta
    10621 gctttacatt tgaattatta tcgtgaaatt catggtatat ctgttcgacg gttgaaaatt
    10681 ttataacatt gttaacattt gccaaaatcg gcgtcttacc atctactgat tttttgccag
    10741 tcaaaccctt gacatcgact ttgccgtcct ttgtaacacc aagatagttt ttcttcctgt
    10801 tgcttagtac gacatatcgg tattccttgt ctacttcaag ctctactcca tgatctcttt
    10861 ttgcgccatc gattaccttg ttgatttgtt ccgatgttgg attttttaca aataagctat
    10921 ccgtatccga atataacact tggattccgt tttcttggca tttcttgatg gtttccatga
    10981 ttatgtgcct gccaacagct gtcgttgcct cagctgcggg aagaaaatac agaggaaata
    11041 tttctgcacc catcacccca tagctggcat ttagaattac tttgagtgct tggcttacca
    11101 cagtatacag ttctttttgt tccagagtct ttgccttttt tgccagactc ttgtaataat
    11161 ttacgcgcaa gtcgcgaaga gagccaatga gcatcgatgt cagcccgttg tattttgtgc
    11221 agacccagtg gtttgtgtca ggtatggtat tttttttgca ctcctcatgt gagcacctca
    11281 atgtctcata tgagatattt ctgaccttga ttatacttgg atacaggctt gcaaagtcca
    11341 tcacggtgac atcaaagtgg attccctcta ctggatcaat taccagtcca cctcggtatt
    11401 ttttgtcctt aattattgcc tcattgacta caccctgaga cctggcatcc agctcctctc
    11461 ttcttggaat cagggcgttt ctctgcctgt gttcatagta taggaggctg cgaatccact
    11521 gcgatactcc catccttgcg atatcatcta ttggcattct tgcaattctg gatattacta
    11581 cgagcaggtt catcagcaaa tcgttgttaa agctggtaag cttgtatgtc agcctggcat
    11641 cgttgtagca atacgcggca agctggtagt ttgtcagctt gtcaaactct acgccaaagt
    11701 caagttttga ctcacttagg agtgcctttg agactgagtt gagtgaaaaa tcagtgtact
    11761 tgtgggaaaa cgcatagatt tgaaaggagc gattcgacat ggtgcgatac aagtcgatgt
    11821 ggactccgtg ctttagtgtg gcagagtccc tcatcatgta cagcgggttt tccgaatttt
    11881 ttatgccaag cctttcagca cggttgtaca ggtatggcat atcaaagtcg tcgccgttga
    11941 atgtaattac aaaggggtat tccgaaatta atttgaaggt gtcctcaatc atcttttttt
    12001 cgtctgactc gtcgtaagtg accatcttga cgtctggtgg aagctcgttt tttccgtcag
    12061 ctgagcccgg ttttttcaaa ataaagatct gctttagtcc gtctgagcta tcaaatccta
    12121 cggcagtgac gcgccgctct gcaatttttg cgtctggaat ccttcctatt tcggcatcta
    12181 cttcgatatc aaagctaatc ctccgtatct ttggtattgg ctggtttagg agctccgccc
    12241 attgtgtgat gtagtcctgg aattcctttg tgttgactat tcccttagtc gtgtttacat
    12301 caagcagcag gctcttcatt gccaagctca cctcttctgt tacctcaaag ttgtgtggaa
    12361 caattttgcc gtcttgtact ttgtaaaacc tgcctacaat gaggctcctg tcatacagat
    12421 agttttcgta atattttatg tcggactccc aagtctctat tatgtttctg atactttttt
    12481 ctgtctgggt gccgccaatg gcaagcgggt cagttacaat tatttttgag acgaggattt
    12541 ctttgtctct gagcaaatcc tttcgtttta tctgctcgat ttttatgatg tcctttcttc
    12601 cttcaagaaa tccaagctca tctgggttca gctttgaata gcagtatggt ttgtggccag
    12661 tctcgtctgc ccaaagaata attttctgag agtccggatt gtagaatttt agcactgcgg
    12721 cttgtttctg gttatcatat gttgctgaga ctaggagcga tggcgaaaga gatttgattt
    12781 gcggttcttg ggcctgcatc gtatcttacc ttttttgctc agagagcaga gtgcttgccc
    12841 agtactggat tcttttttta tcaagcgtca cgacgtctat tcctgcctcc ttgatcagat
    12901 catagtcggt ttctgggtat gtgtcaaggc agactattct tttgatgcct attgttattg
    12961 ccatctttgt gcattcaagg cacggaacaa atgtggtgta caagattgcg tcctttgttc
    13021 cagcctcgat tcccataatt gcgcagtgca ttatggcgtt tgcctcggcg tggttgcata
    13081 ggcagcggtc aagcgattca ccagacgcta tctttccctc cattcgaagc tggcacctct
    13141 tgcagccgcc ctcaaagcag tttttcacgc caggaggcgt tccgttatat ccagttgcaa
    13201 tctgcctgtt gtttctaaca attactgcgc cgacctgcct tgtcatgcag ttggatcgaa
    13261 gctttgccag ctctgcctga agcataaaat attcgtccca acttgggcgc tcaaaatttg
    13321 ccttcacaaa gaaatcatga tctctttaat atttagatgg atccatttgt gtttcaccga
    13381 taattaacta acgagctact attgctcact cgaaagtata aaatgcgatc tagtctgatg
    13441 tatcaggtaa ttggcagact acatacaaca caagttcatc aagcccaaca cgattgaggc
    13501 acgcgaatac caaatcaatc ttgcaaacca ggcaaaaaca gaaaactgtc tagtcgtcct
    13561 cccaacagga ctagggaaaa ccgccgttgc attacaagta attgcagacc atctatcaaa
    13621 aggggaaaac ggagttttgt ttttggcgcc aactcgagtt ctggcaaacc agcattacga
    13681 gtttttaaaa aataacatga atctagaaga cattgcactg cttacgggag aagacaccct
    13741 ggcaaaaaga aaaaagcttt gggcaaacag cgtcatttgc gcaacgccgg aaataacaaa
    13801 aaacgacctt gacagaaaaa tcatcacacc agaacaattc ggcctagtga tatttgatga
    13861 ggcgcacagg actgccggtg actatgcata ctcgtcaatt gccaaaagac ttgcagcaac
    13921 tagtgcaaga ataatgggga tgactgccac gctcccaagc gaaaaagaaa aggcaactga
    13981 aatcatcacg acactcagga ttgcaagcgt agctcaaaga aacgaggaaa gcccagacgt
    14041 caagccgtac atccagcaaa caaacaccga gtgggtcaaa gtagaactcc cgcgtgaaat
    14101 gaaggaaatt caatactacc ttcgcaaggc actcgaggag agatacggtg agcttcaaag
    14161 atgcggtctg cggcttggca caaactcgtc tttatcgcag ctgctaaggg cccgtgattt
    14221 tgtcctgcgg caaaacagga gggctgcaaa accgcttttt accgcaatac gcttgcacta
    14281 tgcgctaaac attttagagt cgcacggcgt gacttcgttt cttaggtttt gcgagaggac
    14341 caaagaaaaa aagggtgttg gaataaaaga tctttttgag aacgactcta attttcttag
    14401 ggcgctagca catgcaaaag atgcgcagaa aaaaggaatc gagcacagca agattgtcaa
    14461 gcttgcagaa ataataaaat caatccaggg aaaggcaatc gtctttacaa gctatcgtga
    14521 ttcagttgag ataatccata gcaagctagt cgagatgggt gtctcagcag catttctgat
    14581 tggcaaggca ggagagacag gactaaaaca aaaagaacag attgacacgg tacaaaaatt
    14641 ccgcgacggc atctacaacg ttttggttgc tacgcgagta ggagaggaag gactggacat
    14701 ctctgaggta aatgatgtca tattttttga caacgtccca agctcaatta ggttcgtgca
    14761 gcgaaagggc aggactggca gaaaatcgat tggcaagctt acagtactta ttgcaaagga
    14821 cacaatcgat gaggcgtact actgggtcgg gcaaagaaag atcaaagccg cacaagggat
    14881 gggcgacaag ctaacaaagg acctgcaaaa acaacaaaca aaatctgtct tagatgcgta
    14941 tttttagtac attgtaatga tgtggtaatt tcataataac cagctattct aatattcata
    15001 ttttcaaagt tgctacaaat gtggtaggat ctgcaagaat ggatatgcag accgtctgaa
    15061 aagcggttca tctgtggccc agatgtcgtg ggttcaatcc cactcctacc agccttatgg
    15121 attttggaat attaaaattg attgtttgaa ttcagggttc ttctatgcga gccttttgat
    15181 aatgtggtag ccatactctg tctttattgg ctctgagagc tggccaactg aaagcttgaa
    15241 tgccgcagcc tcaaacggct ttaccatttt tcctcgtcca aaatacccca agtctccacc
    15301 gcgcttgccg ctgccgggat ccaaagacag ctgttttgca atctcggcaa acttttcacc
    15361 attctttagc ctctcaagta ttgcaagtgc ctggctttgc ttttcgacta gtatgtgtgc
    15421 gcattttatt ttatcagaca tgattttctg aaaaacagac ggcacaaaaa tatcttatgt
    15481 tccccatgag gtttttttgg ttgggattag cttcaaaaac ggcgcctcgc cttccttcca
    15541 tgggtctcgt cgcacccact cgaatttttc aaaaaacttt tcatagatct ttttaaactt
    15601 gaggcctttt tcaactatct cgatttttcc ctgcgctaaa accgccttgt ggccacccgg
    15661 cttgtatata tcaactgaga ttgcggccct tgggttcttt ttgacgttct tgaaagtcct
    15721 agtagaataa tcagtggcaa cataaaacac atcgtctaag aaaataaacg atactggctt
    15781 tacgtgcggc atggaatcgt ttgcagtcgc aagcctggcc tcctcaagtg agccaagaaa
    15841 ctttttctcg ccttcagtga acttcaacca aaaatccttt ccctgacttg ggggatcttt
    15901 gagtagacca tgtccctgat tttttgctgg tctgcctctg ttggcatccc tcctcttagt
    15961 gcggcaattg ctgcgcttgc catgcctagg gccattccca tcacagtccc gtatacgaat
    16021 tcctttacgt ttccaacctt tagttcggct ttgttttctt cgatttcctc caaatatgcc
    16081 ggaattgact gcaatgcccc atcgagggtt tgtatgatta gttgttcaat ctgttcgtct
    16141 tcactcataa tttatgtccg tcacattcag taataaattt gcacatcttt tttacaaaaa
    16201 tacttttaat gccgcgcatt tgatactcaa acattgcctt cgtattttac catagcagct
    16261 gcaaatgagg cccttccaaa agtaattgag aaatacaaaa acctgcaaaa acaaaaacaa
    16321 gaggtacaaa agctagaagc gcagctcaac acgcaaatgt ccgtttcatt taaccttgca
    16381 gactatgttg ccataaagca acagctcaat gccgccgtga caaaattcta ccagttagta
    16441 gaagacctag aaaacacagg agtggtccta aaaagtctgg aagaaggcct cttggatttt
    16501 ccatcaaagc gctttgatga ggaggtgtgg ctctgctgga aggaaggcga aaaagaaatc
    16561 aagttctggc atgagaagga tgtgggtttt ataggcagaa agccattgag tgtaagcgat
    16621 gaatcacttg tatgagtctg tctacttggt ttagaataat tcgcgtaaag ttcctcctag
    16681 catctgtaat tgctgtatca ctaggattgg cagtatctta ctggcagacc cagaggcttg
    16741 atgtggctca ctcgataatt acgatggcag gagtgcttgc cctgcacgcc agtgtcgact
    16801 tgttaaacga tttttgggat tacaaaagag gcattgatac tacaacaaag agaaccaagt
    16861 ttagcggcgg aaccggggtc ctgccggaag gactcctcga gtcatcgcac gtatatcgag
    16921 ctggcgtcat atttttgatt cttggctcgc ttgcaggaat ctatttcata gttttgtacg
    16981 gctggattat cgctgcaatt cttggctttg ctgttttgtc catctatttt tactctacaa
    17041 aaatagtcga ttcgggatta ggcgagatct ttgttggaat aaagggaaca atgattgttc
    17101 ttggaacaat tttcattcaa acacaaggca tcgagccagc aaacattgtt gcaggagtaa
    17161 tagctggtgc gctttcatca tttgtcctgt acatcacatc gtttcccgat tatgaagcag
    17221 acaagcaaaa aggaagaaga acactggtga tagttcttgg caaaaacaag gcatctgttc
    17281 tgttttgggt gtttccagct attgtttatg gtctaatagt atatggcgtt gccactgaaa
    17341 ttcttcctgt tttttgcttg gttgcatttg caacaatccc actagttggt attgggacaa
    17401 aaataaaaaa aacacttgat gatgctgagc ggttcgtatc aataatgaag acgacactgg
    17461 tctttagcag aataacgggg ctgctcctca ttgttggatt tttaatagaa ataatcaaaa
    17521 acacttacag ttaaatcagc aagtttcgga tccacttaca tgctttctgg gtttgccacg
    17581 ccaaatggaa caaaaaagtt tgcagaaaaa tttggacaac tccaagacaa ctacaaaaca
    17641 tttcaagacc tgacgctatc taatgttgga attgggacat atcttggcga ccaagatgat
    17701 cagacagacc tgctagtgac tgccgcagta aaaaaatctg ttctctctgg aatcaacgta
    17761 atcgatactg cgataaacta tcgggcgcaa aaggcagagc gctcagttgg taaggcagtc
    17821 tccgagcttg tctcagaagg caaaatcgag cgtgatcagg tctttatcag cacaaaaaac
    17881 ggctatgtca caaacgatgc tgccatcaaa gaagagtttt ggagctacgt tcagcgagaa
    17941 tatgcaaaac ctggaatcat cggaccaaac gatatttcat cggggtacca ctgtatgact
    18001 atcaagtatc tcgaagacca gctaggccga agcctcaaaa atctcggact ggaatgcgtt
    18061 gatcttttgt atcttcacaa tgccatcgaa gggcagatac aggacatctc aaaagaacaa
    18121 ttcatcaaaa atctaaagga cgtatttggg atgtatgagc aaaaacgcaa ggagggaaag
    18181 atcagatatt acggcatggc aacatgggaa tgttttaggg tacaaaagga aaacccgcag
    18241 catctgtcgc tatctgatgt gatccgtctt gcaaaagaag cgggagggga agaccacggg
    18301 tttagattca tccagctgcc atacaatctg tactatgatc aggcacttat gctaaaaaac
    18361 cagccaataa gcggagacga agtctcaata cttggtgcag ctcagaacta tggggttggt
    18421 gtatttacaa gcgttcccct gatgcaggga aggctgcttg caccgggcgc cttgccagat
    18481 tttgatgggc tcaagccgtc attaaggtgc cttcagttta ttcgctcaac tccgggtgtt
    18541 ttggcgccac tagtcgggca aaaaaatcca agtcatgtgg atgaaaattt ggaaatcatg
    18601 aaaatccctc caatgaaaaa agacgagttt gaaggcctag tcaaaaaact ggtttcctag
    18661 ttttcgtctt ttacttttcc gccaaagtcc aaaatagagc ttgatatgtc cggaacagtt
    18721 ctctctatgt ccaaaattac accatcgttt tccacgactt gaattcggat gtattcgcca
    18781 gttgatggcc tgtccttgtc tgcatacaca acaactaggc ttgcaatctc gttgttttgc
    18841 acattttgat caaaatcctg gtttatgacc cagtaaagaa atgcagtcgt agtgtccggt
    18901 ttttgctggt ctgcttgggg atttatcgca atgagccctt tttctttggc tgcaatcagt
    18961 gcagaattca acgagttgtg ggattctccg tacagggaac ctgcgtaaat gtcatcatgg
    19021 gtaatggtgt agctcccatc ttttatcagc tggtaggtca ccctcacatc ctccgcactc
    19081 atatcgacaa ctccgcttgt agtggcagtg ataggtgttg cagttgcagt tactttgtta
    19141 tttgaaacct cggcagtccc agccattttt ccaatgattt gcagcgtgtc tgccgatacc
    19201 actgcatcct ccacagtctc ctttactgcc tcactagtgg caaaaccaga actcatgaca
    19261 gtccaggtaa gtccgatcgc aactgctgca aacgtgatca gcgcagcgtt ataccaaaag
    19321 gtaaggccac gatgtttttt ggcgagtcta tccaacagat tttgtagtag tgcaaatctt
    19381 atctattgta gtgtgtttgg gccgaacgag tgaactactg ctgaacctgt ccagcagcct
    19441 tttcctgctt caacgaccga gcttttgatc tctgcaggcg tgaattcccg tagttcctac
    19501 ggtaatctgt ccaacccaac ctgtttcggc tttacatgac atcagatatg ggcttgtatt
    19561 gttgtcaaga ccctctttta ttttgcctgg ctctaacatt gtttatttta tgaattcatt
    19621 gcatttagca agctggttat tatacaataa ccgcgattca tcagtcaaaa caagtgatct
    19681 actcgtaatt tttataacaa aataatctgg atgagcatat gtcagagcta ccaaaattcc
    19741 caagccgcgc atttttggct ccaatggcag gcataagcga tgttgcactg cgacttttgt
    19801 gcagggagat gggtgcgggt ttggttgtaa ctgagcttac tagcattcat gccataattg
    19861 caaaagaaaa acagcttact ttgcacaata aacaaatcag cgagttcatc gagttttcag
    19921 aaaaagagcg cccaatctca gtccagttgt ttggctcaga ccttgacgca ttacaaaggg
    19981 cagcaaagat agtagagcca ttttttgata tgattgacta taacatgggc tgtcctgcac
    20041 cacacatcac agaacaaatg gctggaggcg cactgcttca aaatattgat cttaccagaa
    20101 aaatcttcag gacgctgacg gcatctgtca aaaaaccagt aacactcaag atgcgtgcag
    20161 gagttgaaaa caccggactc ttcaaagaaa ttgcgatggt ggctcagcaa gagggaatca
    20221 aaatgatcac attgcacgca agaactgtaa aacagggcta ttcggggatg tcaaactggg
    20281 atttaattcg ggatctaaaa gaactagtgg atattccggt ggtgggaaat ggcgatatca
    20341 caaatccgga gcttgccaag caaatgatgc agcaaacaaa ctgcgactat gttatggttg
    20401 gacgtggcgc gatgggaaat ccatttttgt tcaagcagat taacgactac cttagttctg
    20461 gctcatacca gcactatcaa ctaaagactc ggcttgaaat atttaacaaa tacatcgagt
    20521 atgcgataca ttacaaaatc agattttcga gtataaaaca acaagcgatg cgattcacaa
    20581 aaggcattgc agatggcaca aaactccggc cgaaaattat gcttgcaaat acaattgatg
    20641 agctaaatac aataatggtt gcggcggcat aatgctccag ctgatttgat ggttcattat
    20701 gtataaatag aaaaattgtg aggcctactt atgccagttg atcccgtttg cggaattgaa
    20761 atggatgaaa gtttggccgt aacttttgaa tttgatagca agaaatactt tttttgctgc
    20821 aacgggtgca ggagtatttt taaaaaaaag ccaaaaaaat gggcaaaaaa gacctagagt
    20881 ggaaacctgc cacacattct acaaaatttt gaatcctcgt caatcatatg atgacaatca
    20941 ggacactttg tacccgcttc atcaaagaca ggcatgtcgc aatatttgcg ataaatctcg
    21001 taatactgca gcgattcttt tgttctaatc gtaattccgt cattttcttt gatttttttg
    21061 gctttttcgg caaatacgtt gtctgggatg gtgacttcaa ataatcctgt taatccctgt
    21121 gttcctgcgc cgtcttgcat gggggatttg gagcgccaag cgattgatct tggaatcttg
    21181 tcctcaaagg ccttcctcaa tatccacttg ccaaactttt tttcattttc ttggcgcacc
    21241 atgaggtcaa ctggtatggt ttttgcaaaa tcaatcacct ctttggtgca aaaaggcgac
    21301 tccacaacaa ctcccaagga cttgccaatc ttttgtgatg gaaagtgcat tatttttgat
    21361 atcctctgaa gatcgcgctc aagatcgtct ttgctcttgt ttagcagaaa attatagcct
    21421 gcaaagattt catctgcccc atcaccagtt attattttgt caaatccaag tttctttaat
    21481 tcagatagcg caagataaat tacgacgttg tttcgtatct caatatcatt aaagtttttt
    21541 aggattttta ttgtttcttc aactccgttg tatatttcgg cggcatcgca cattttgatg
    21601 tgcaaaggca agccaaatct ttgtgcggca agctggcagt atgtcaagtc atttgcaaca
    21661 aaatcttttg aaatgatcgt tatgcaacta gtttttctat tcttgagaaa gtacgcaaga
    21721 attgtgctgt ctagtccgcc agacaacaaa aggtgctctg ccgtacactt ggaagctgct
    21781 ttttctaaaa tttcatatgc gctatcgata atttcttgca cgattttatt acaaatgaaa
    21841 acaaattaaa ttttggtttg atgtctgcag ttagcttgag ctattttgga tgaatagagt
    21901 ttgcgttttt gatttcaaat aactagcgtg ttaactttaa atagattata agagaaattt
    21961 tcatattgct acttccagca gaaattgaat cgaaaacact aattcctgca cttcgggcca
    22021 tcctagccaa aaagcttgct gaagaacaca acgtaagaga agacgaaatc tcaaagatgc
    22081 tcggagtcac tcaagctgca atcagtaact atatccgcgg aacgagggga gacccaaaac
    22141 taatccaaaa attagttacc gacgagcagg tgtctcagct gataaatgat ctcagtgaca
    22201 gactggcatc cgatatggcc tacaccccat caagtcttgc caaattcata agcctttgta
    22261 actacatcaa gtcaagcctt ttgatctgtg agattcacca caatctggaa tcaaacattg
    22321 atgaggcaat ctgcaaggaa tgcgagaaca tgctgttaaa aggccctggc agtgtttatt
    22381 agattacttt ctgaccagcg aaatttctat tgacgataac atctttttgc catagatgtt
    22441 ttcagaatca agtgtgatgt gctcaactgt gcagacgcga ttaagcattt tttccactat
    22501 gatatttgcg attgcaacag cacttggaat tgagttgccc tttgctaaca aaatgctttt
    22561 tccatgtttg ttgattagtg tgaaaacata aagtgcggcc tgcattaacg gctcgtctct
    22621 tacatagtaa acatacggct cgttttttgg agacacttga tcggggtttt gtatcccctc
    22681 catgctaatc atcgaactct ccaaaaattg cagttctttt aagttttatc gacgaattat
    22741 ccattagttg aaataatcga ttggaatgtt ttggtaatcg ccgtttggca accttcttct
    22801 aatgacaatt ggtattgccc tgtcctcgag ttcttgtagt gctatgtcaa gtgatgttcg
    22861 tgcagttgcc ggaatctgta tgaatggtgg tgcgccttga gaaagttgta gtgctcgtgc
    22921 ccccaaaatt cttgcctttt caaatcttgt gaggactggg gggcctattg ctattttgcc
    22981 ctttcccgct agtggaatct caactattac gtgaattggg tcaacatcaa ttatttcacg
    23041 ttgttccatt gccttgattt tcttgtcaag ttcggcccgt tcgtcatctg tgatctcttt
    23101 agtttcaaaa attttcttgt atgcatttat tgcatcctcc atcgaggtgt cttcactacc
    23161 atcctcaact actggcctaa caaactctgg ttcttcttca acttcttcaa caacctctac
    23221 ttcagcttct tctggttccg acaagcaaat caactctctg gtacggttat atattcataa
    23281 gcaaaagttt ctagaatgag cgccggttcg gtatcaagat cacatcttat tgtggaagta
    23341 aaaggcaaat caaggatgac atgtgagcta aagcgccatc tttcgccaaa aacagtcggc
    23401 tcaattttac gatctttgcc acttgaagga aatgcgcatt ttcttggaca aaacatcgtg
    23461 tattttgaaa ccgcagtcaa ttccggagtc gaacgacaac gaaaagagtt caaaaaagga
    23521 gatattgcat tttcacctgc aggtggaagc atttgttttt ttatctcaga tgtagtctca
    23581 tcaaaagcaa tgacgccaat tggcaagtta ttgtctgatg ttgacgcgat tagtgctgta
    23641 aaaccaggag atgttattgc ggtttatcag caatctgctg atatatccag tggccactaa
    23701 agccgcctgc tcgttttact gatccttttt taaccaaatt gacaagacat gcgtttgccg
    23761 cagaaatttt tacaccagtt tgctttgcca gttcttgaac agtcaaatag ttatttgact
    23821 tgattagctt gagtgcctgg tcctcattta ccagtactgt gatttcggcc cgtctttcgg
    23881 ttttttctcc ctttttgtct tttttacctt gactgtcttt tttggtcttg tcagtgtctt
    23941 gcgatttttc catctgtgcg gccgatttct tctttgttcc acccataatt tttgtgctaa
    24001 ttttgtccat ttataaacga ttgatagcac aaacacaaaa ccatcatttt gttagttaag
    24061 aaaaaccgga aaaactgttc gtacatattt tacacactag gattagtata tcatattatt
    24121 gaacgaattc cagattgact cgaaggtatc tcgataaaaa gaccaaagat cgcttcaaac
    24181 gactagtacg gcacgacgat ggaagcgaga acaaaaaata tatcacagaa tcgggcccaa
    24241 aggttccaga gtttgaggaa gctgttcaag ccaaaagaat gatcttcctc agagatctag
    24301 ggggagatgc gcaatctgcc gatgagatct taaatgagaa aataggagag ctgttaaaca
    24361 ctgcacagga aaccagtgac tacatcaaaa acgtcaacac acatttggac aaacaaaaga
    24421 ttcgagtcga tgagctaaaa aaactgcaaa aaatgtttga aagtcaagtc tctgctttgg
    24481 aaactaaaga taaacaaaaa tcaaactcgg attaatatta tcaaaaaata attatcaaaa
    24541 aatgaatatt tgttattcgg gatttatctt gaatgtttgc tcaatgcgat agctatctgc
    24601 gactgttcaa atggttttaa gatatagtca acggcgccag ctctcatagc atcctgaaca
    24661 atgtggctct tcccagtaga tgaaatcatg atcactttgg catttgggtc aagctttttt
    24721 attgcacgca gtgcctgtat tccatccgcc tttggcatca agacgtccat ggttacaagg
    24781 gtgggcttgt gctctttgta tttcatcact gcctccacgc catcaccagc ctcaattatt
    24841 atgtttgaga gcctattctg ctcgataatg tttcttattg cccttcttga gaaaggcgaa
    24901 tcatcaacaa tcaaaatgga aggctgccaa ctagtagtac tcattgcgga cactccctat
    24961 tggaatttgc aagcgcgtag agaatgtttg ttttgttgat tagccccact agttggccct
    25021 ggctgttatt tatccccaaa caatctattc tcttatctac tagtatcttg gctgccgtct
    25081 ttacgttggt atcggagtct attgttatga tgccttcagt cattgtctct gcggcaagct
    25141 gagagcctac aaatccagtc tctgaccaca gtccatcttg ttctgtgtat ctactcaggc
    25201 ttgattgaac tatgtccaag ttgctagtcg tcatggtgat cttgaagata tcgccatttg
    25261 tgattatgcc aacgggatta ttcgcactat ccgttaccac aatgcgcgaa atgtcgtata
    25321 atatcatttt tttgataatc tcatataatc tgtcatcagt tctggctgaa aaataactca
    25381 ctgtcatgta atctgaaacc ttgcttttgt cctgctggtt attcacatag tatttgagaa
    25441 gatctgtctt tgtgactatt ccttcgaggt tttcttttga gcctattgcc agtgtgctta
    25501 ttttgttaat gatcatcatt tgtgcagcct gctggcatgt catattatca aacacaaaac
    25561 aaacactatt cattatttcc gcagcataga cgctatcaag acttctgcca cttttatcga
    25621 tgtacaggaa ccttatgatg tccttgtcgg taataatccc aacaggcctg tcgttatcaa
    25681 agacaaagat tttgctaatc ttatttgaaa ctagatttag tattatgttg tgaagagtgc
    25741 tgtttatctg caccccgatt atacccgtgt tttttatatc gtgtattgtt ttgacaattt
    25801 ttgaattaac attggcgtat tctgaatgag acatactatg atctttccaa gcaaatcata
    25861 attttattac atagttagtt tgatgtgaac aaacaatttc ggcgatttta cagtgaattt
    25921 gaactatggt tactgatatt ttggcatgaa ttattgcgca gaggctaaat accatgcaaa
    25981 atatgtaccg atatggggat tgtcgcaaaa ggcgccaaat gtgacgttga aggctgcgac
    26041 aaagacggca cgcgctcact aggttcctca cgagtggaag ctgcaggcct gcgcctcaaa
    26101 tcctcaggca aaaaggcagt tttatgtaaa gaacactaca aagaatggaa aaaggaaaca
    26161 aaagaagacc gcgagcttga acgagccaga tacgacaggt tctagtcttg ttcgtagttg
    26221 ttggttttgg cgtttttgag aaattcggtt tgtagttttg tgtagaactt gttcaggttt
    26281 ttgtagattc tttttggctt tcttggcttt tgcgtagcta atacatacgc cgcaataatt
    26341 ggaaatgcaa ttgtcgaatc agtgtacacc gtaactagtc cttcatgcga gtcctgcacc
    26401 tttccccaac tttttccttc ttggagcgtt gcgccagata atccacccgt atctggtctt
    26461 gcatccgtga tttggattat gtagtcttgc ccgccatcgt gtcttctaag gatttgatca
    26521 agcatcgggc ctgtctgctg tgcagtgttc tttggaactc ctccaccgag ctctaatatt
    26581 ccagattttt ttgaattata aacaattgca gcctgttcta gtacttcacg aacaaagtca
    26641 aggctgtaga ctttgttatg tagcctatgt atgcccaagt tgagtgcaag cgatgaatct
    26701 tttagtgttg agacataaac tggtacatca tacttgtatg cagaaacaag aaagcttttg
    26761 tccggatgtt ttgctttttg actgctaacc ttgcccataa aattacaaaa ctctgcagtg
    26821 gtaaaaggtg catcagttag ttggtttttg aacatttttt ggatgatttg atcttcggcc
    26881 tctaaggtct cacagaattt gatgtagacg tcccttatcc gaacaatttc tttttcatac
    26941 aataccatgt catccacctc aaaatggcct tgttttacag gaagccccca cgcaaaatga
    27001 tcctcatggt acacgtttgc accagttgta attatccagt ctacaaaccc tttttcaatc
    27061 aatgttttga taattcctcc aaacccaacc ggtgtcattg cacctgatac tgtaaggcag
    27121 attgtggcac cttcttttat catctgggca taaagctttg cggcatcgcc taactgtctg
    27181 ccattgtagc cagagcctgc aaagacatca acgagttctg aaatcgttgt tttttggttg
    27241 agcctgatat gcggtatgtt ttttccatga aaatggtgat gatccataat ttgggttttt
    27301 tttaatagta taataattat tttattgtaa ttgaaatttt taaaactgtg caagatcttg
    27361 atgaaaatga ttgaaaataa tcgatgttgc caacccagaa agaattaacc gaaaaccaga
    27421 cagaacaata atgcttcttt cttctggaaa tttggcagaa cagggctatg ttattaaaaa
    27481 aatcgagtta aggctatttg ttgaaaaaat agatcaaaaa ttagggccat tttctttgat
    27541 tacgtcatta gttgaaacag acaaaggcga agtcgaaatg atgtatgatg agggctatag
    27601 gggagaagat gtcctagaaa aaacagcgaa ttttatagta aacaatcttg gactttctgg
    27661 catgatttta cgctccttac ttgtactcca atccaaatag taagtttgct tcatacaagc
    27721 tagtgcctat gtgcgtacta tgagaatatt ggtagttgtc attaagaata ctaatagctg
    27781 atgacgaaaa agacaatgcg gattcatatg cagaaatact acgacagcga ggacaccaag
    27841 ttgttgttac cttcagtggt gtagattgta taactgaata tctttctgca tttgactatg
    27901 agcaaaactc cccatatgat gttgtaataa ttgattactc tatgcctgac atgagtgggg
    27961 tacaggccgc cagaaaaatg ctggatatga acccaaaaca acaaataatt ttcatttctg
    28021 gatatggttc agagctgtta tcacacctag atgcattcca taatgttgat ctactgacaa
    28081 agcctatcat tcctaacgct ttgatcagat tagttgagga ttagaatttt cattatttga
    28141 ttatgtgccc aaattattat gcatcacgta attctacaaa acatttaagt caataacaac
    28201 aactgtttga aattgtgcgc atactacagc ttcattgcga tagcatcgag tttaccccca
    28261 caaaaaaaga gataaaatcc gcagaagaga tcgagccggc gacaaaaaga tttgaagaaa
    28321 tagtcgttgc atttgtagca atcgaagacg gcgatgattc agagatagca aaaaaagcta
    28381 tagctgagat aaaatccagc atggaaaaag ttggctgcaa caaactgctc ttgtatccat
    28441 atgcacacct aagctcgaat ctggcgtcac ctggcactgc actaaactta ctaaaagaaa
    28501 tggaatcgct ggcaaactgc gaagtttcgc atgccccatt tggatggaca aaatcctaca
    28561 acattaaagt aaagggccac ccgcttgcag aaagctcaaa aacaatcaca aaaggagaac
    28621 atgaacacac atcagaagcc ctaaagtcag agtcaaaaat ccagtcgttt tggcacattc
    28681 tgacgccaga cggaaaactt cacgatgttg gcacgtttga tttttcaaaa cacaaaaaac
    28741 tcgaggtttt ggcaaaatat gaaacagcaa aaaaaagggc agttgatgag ccaccaccac
    28801 atgtcaagct aatgaaaaaa atggcaattg cagactatga accagcctca gattctggaa
    28861 acatgcggtt ctatccaaac ggccggctga taaaatcact tcttgagcgg tttgtcacag
    28921 acgaggtcaa aaactatggt ggattggaag tggaaacccc aatcatgtat gactcacatc
    28981 acccaagcat ggagagttac tttaatcgat ttcctgcaag acaatacaat atcaacgcag
    29041 aaggaaagca gttgtttttg agatttgcag catgctttgg ccaatttctt atggcaaatg
    29101 atttccaaat atcgtacaag aatctaccgc tgaagctata cgagctaacc agatatagct
    29161 tcaggcgcga acagtccgga gaactggtgg ggctgcgaag gcttcgtgca tttacaatgc
    29221 ctgactgtca cgcattttgt gcagacatgc cacaggcaat agaagaaata agaaaacgat
    29281 tcgacttgtc taggctagtt ctaagagggg ttggcatagc tgaatccgat tacgagatgg
    29341 cgattcgctt tactgaagag ttctataacg agaacaagtc cataattgag gagcttgtca
    29401 aaaagattgg caagccggtt ctagtcgaga tgtggaaaga aagattcttt tattttgtac
    29461 taaaatggga gtttaactac gttgataatt tgggaaaggc gtctgcactt tctacagacc
    29521 aaattgatgt cgaaaatggg gagcgttatg ggatcaagta ctacgatgag aataatgtgc
    29581 cgcaccaccc aataattttg cacaactcgc caagcggcgc aattgagcgt ctcatatacg
    29641 cccttcttga aaatgcggca tttgatcaaa aacagggaaa aaagccgcac ttgccgctat
    29701 ggcttgcacc tacacaggtc agaataatcc cactaaaaga agaatttcta aagtactgcg
    29761 aggatcttgc cgacaaaata agtcaaagtg aaatccgcgt agatattgac gatagaaacg
    29821 aaacaatcgg aaaacgaatt cgcgagtccg aaaccgactg gatacggtac accctagtaa
    29881 ttggagaaaa agagatcaat caatcaaacc tttctgtgcg agacaggtct tcaggcgatg
    29941 tcaaagaatt gtcgtttgat gggtttatca aggaaatcaa ggagcagaca aaagacaaac
    30001 cgttttcaaa gctcaacttg gcaaaatatc tatcacagcg gccgcagata atggtctgac
    30061 ctgtttgttt gaaccaaaca cctcaaagta aaataacgaa actctagcaa tactttttga
    30121 atcaaatggg attgaatatt cttgttgcag aagacagtgt ggcatacgcc gttttatatc
    30181 agcagatttt tgaatctcgc ggccatactg tcaaactgac tttggatggt gaggagtgca
    30241 tgtctgctta tgcacaagaa gcagcaaaaa agcaagattc tcagaaccca tttgatgtag
    30301 tcatacttga tcactctatg ccaaaaaaaa ctggttttga tgtggcaaaa gaaatccttg
    30361 aaaccaaacc aaaccaaaaa ataatgatta taacgggttt tgatgacgag atttctgcaa
    30421 aactaaagca aagtgacatg ggaaaaaata tcgaagtgat cgaaaaaccg tttaactcgt
    30481 cagttctcat aaacaaaatc gagcgggaaa ccatgaaaga aaaagcaagt cacgttctga
    30541 tcagctaaac tggccttgag tattcccatt catttatttt gtagtgattt ttgagtagtt
    30601 tcgaatatga gctaccttga cttgtacatg aacaaggctc cgctaatcat tccaccaaat
    30661 gaggaaggtg aaccggttgc tattgttttt ggcatcccgt ttgattctac acattcctac
    30721 aaaccaggaa caagattcgg gccagatgca atccgagacg cctttaacaa catcgagatt
    30781 tttcatccac agttaggagt tgatttggag tccgttaaca tcgaggacct tggaaatacc
    30841 aagcacaccg taaatgtaag tgagatgact gacatggtaa acaagataac cggtgaactt
    30901 gccaaaaaaa acaagctgct tgtaattttg ggtggtgagc attctctgac ctatgggacg
    30961 tatactgcat ttccaaagga tactggttac attgtctttg atgcgcatta tgacctaagg
    31021 gatgggtatg cagacgttca tctaagccat gccgcatatc tgaggcgggt tgtggaaaaa
    31081 cacggtgcag aaaacatcat tcatgtgggt gctcgctcct ttgtaaaaga agagcttgcg
    31141 tttcttaagg aacacaacat caaaacaatt tcagacaagg aaattcgaaa tggcgatggg
    31201 ccaaaactgg taaaagatgc gctttccaac tttagcaaaa cctatgtgag catagacttg
    31261 gatgtcttgg atcctgcgtt tgctccaggc gttgggaacc cggaggcagt aggaattacc
    31321 tcaagggaga tgtttgatat ggtatatgcg atgggtggaa gtaagatttc ctgcgtagat
    31381 attgtggagc taaacccaac atacgataat ggtgcgacgg catctcttgc agcaaagcta
    31441 atgtcaacaa taattgccat gagcattaaa tagaatcttt atcttttgaa atctaatgct
    31501 aaacaatttc cgtgattcat taaaaccgca ccttgaaaag cttggaaatg agtttgcctc
    31561 tactgggttt tctccaaatt tctggactgc agtgggcctt gcatttgcat tcctatctgc
    31621 aatcgcatat ggactaaacg ggcaaatcca gcacgtggca ataattgggg ggatcttgct
    31681 tttgatttct ggattttttg atgtcgtcga tggccaagtg gcgcgagtta caaaaaagac
    31741 ttcaaaaaag ggcggatttt tggattccgt atttgacaaa atagccgaag tcgcaatatt
    31801 tttgggcatc ttggttggaa attttgcaga gccgtttttg gtgtttttgg caatcacact
    31861 gtcgctgctt gtcagttata cgcgttcaag agcagaatcc cttggcgtaa agctgcaggg
    31921 gattggaatt ggcgagaggg ccgaaagact gctcgtaatt gcgataattg gcatggtggg
    31981 atttatgcag tacgcggtaa ttatagtaat tataattgcg gcaataacgt tcattcaaag
    32041 aatcatagtt actgcaaaaa acattcctga atagtttagt acagacgtcc tgcttgcctt
    32101 ctagagtcag cagatctaat ctttagtatt ccaaaatgaa ttgcacatga aatgcagtac
    32161 catttgagaa cttttggtgc ggcaatgtat gctccttgtg ccctaagctc tcttgcaagc
    32221 gtgtgttcaa ctaaattcag acgtgaggtg actttttttg ccttgtcctt tggaacagtt
    32281 gcaccacagt ttgtgcactg tattcgatcg gaggaaccct ttccgccctt tcttcgtcct
    32341 cggcttgcgc gctttaatgg catgactgtc ctgtaggttg cctacttata ttcttggtgc
    32401 ggtttctact cttacttcgt atacgtcaaa tttgttttca acatcaatcg tataactgga
    32461 agttttttca aagatgtatc cttttatgcc ttttaccttt tcgtataagg actcgccaac
    32521 aatcatggtg tttggttttg caagcgagtt tatcttagaa caagtattca cagttgcgcc
    32581 aaaaatg
//
LOCUS       DASWKH010000042         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1194286, whole genome shotgun sequence.
ACCESSION   DASWKH010000042 DASWKH010000000
VERSION     DASWKH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194286"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VGA92_03210"
     CDS             <1..789
                     /locus_tag="VGA92_03210"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9845455.1"
                     /translation="AKKMGIKKDSVTILIHCGSRGFGHQVCSDYLRISEQAQKKYNIT
                     LADRELACVPNTSEEGESYRTAMFAALNFAWSNRQMITHWTRKSFERVFKQSESDLDM
                     KLVYDVAHNIAKVEKHKIDGQLKSVVVHRKGATRAFPANREEIPRNYRDIGQPVFIPG
                     SMGTSSWILLGQQNSMDLSFGSTAHGAGRMMSRSKARRDYTEEQVKKSLSDKGIFIKS
                     LTRDGVVEEAPQAYKDVDAVVNVSHELGIATKVAKLVPIGVIKG"
     gene            786..1214
                     /locus_tag="VGA92_03215"
     CDS             786..1214
                     /locus_tag="VGA92_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9845456.1"
                     /translation="MSEEDRDLEALKQKRLAEMQKNISYKKKQEDLKSSLEEKEKNKP
                     SPREIVVKQLGHRGLEVLEQAEIQFSSQTKFVIDKLAELISSGEVNETLDGGKLLALF
                     RSIGVNVRLETKINVEQDGKFVSLSDKLSKSAQSNDSSES"
     gene            1211..>1516
                     /locus_tag="VGA92_03220"
     CDS             1211..>1516
                     /locus_tag="VGA92_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845457.1"
                     /translation="MKTVLELSVIKYDEEKECIIKALDELQNLCKITGGYELSNPTSR
                     FGWTFFKIWLRPNLLFCINEKFADMIKKSKGYKSEEKFSRFISDFFESRGCNVKLKLV
                     "
BASE COUNT          547 a          269 c          331 g          369 t
ORIGIN      
        1 gctaaaaaaa tgggaataaa aaaagattct gtaacaattt taattcattg cggctcgcga
       61 ggatttggcc atcaggtttg cagcgattat ctgagaatat ctgagcaggc acaaaaaaaa
      121 tacaacataa ccttggcaga cagagaactt gcatgtgtgc caaatacatc agaagagggc
      181 gagtcttatc gaactgcaat gtttgcagca cttaattttg cgtggagcaa caggcagatg
      241 attacccact ggacacgaaa atcatttgaa cgcgtcttta aacaatcaga atcggatctt
      301 gatatgaaac tagtctatga tgtggcacat aatatagcca aagttgaaaa acacaaaatt
      361 gacggccagc taaagtctgt agtggttcat agaaaaggag caactagggc atttcctgca
      421 aatcgtgaag aaattccaag aaattaccgc gatattggcc aacccgtttt tattccaggt
      481 tcaatgggga cgtcaagctg gatcctattg gggcaacaaa attcaatgga ccttagtttt
      541 ggctccacag cacatggcgc agggcgtatg atgtctcgct caaaggcaag acgagattac
      601 acagaagagc aagtaaaaaa atcgctttca gacaagggga tatttatcaa atcactgaca
      661 agagatggtg ttgtagagga agctccacaa gcatacaagg atgtagatgc agtagtcaac
      721 gtctcacatg aattgggaat agcaaccaaa gtggcaaaac tagtaccaat aggcgtgata
      781 aagggatgag cgaagaggac agggaccttg aggcactaaa gcaaaagcgc ctagcagaaa
      841 tgcaaaaaaa catttcatac aagaaaaaac aagaagatct aaaatcatca cttgaagaga
      901 aagaaaagaa caaaccatca ccacgcgaaa tagttgtaaa gcaactgggc catcgtggcc
      961 ttgaagttct agaacaggca gagattcagt tttctagcca aacaaaattt gtaattgaca
     1021 agcttgcaga gctcatatcg tcaggcgaag taaacgaaac gcttgatggc ggcaagcttt
     1081 tggcattatt cagatctatt ggggtaaatg tcagattaga aactaagatc aacgttgagc
     1141 aggacggcaa gtttgtctcg ctatcagaca agctaagcaa gtctgcccaa tcaaatgact
     1201 ctagcgaatc atgaagacag ttttagagct tagcgttatc aagtatgatg aagaaaaaga
     1261 atgcataatc aaggcacttg atgagcttca aaacctctgc aaaattacag gaggttatga
     1321 gctaagtaac ccaacatctc gttttggatg gacttttttt aaaatctggt taagaccaaa
     1381 tcttcttttt tgtattaatg aaaagtttgc agacatgatt aaaaaatcaa aaggctataa
     1441 atccgaggaa aaattttcca gatttatatc agattttttt gaatctcgtg gatgtaatgt
     1501 taaattaaaa ctagtg
//
LOCUS       DASWKH010000043        15189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1577076, whole genome shotgun sequence.
ACCESSION   DASWKH010000043 DASWKH010000000
VERSION     DASWKH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 15189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15189
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577076"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGA92_03225"
     CDS             complement(<1..513)
                     /locus_tag="VGA92_03225"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9845458.1"
                     /translation="MSTKPSKDARIVDVGGGGSNLVVKLLDLDFSDITVLDISAKALE
                     RAKERLGERAEMVKWIECDITEFDTNDRYDIWHDRALLHFLTSEEDLKNYVELTRKHV
                     KKGGYLILSTFSTNGPMMCSGLDTKQFSEESMKQLFSNGFEHIKSFEEEHKTPFGTGQ
                     IFTCNVFRKII"
     gene            complement(1126..1596)
                     /locus_tag="VGA92_03230"
     CDS             complement(1126..1596)
                     /locus_tag="VGA92_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9845459.1"
                     /translation="MSVADSKNLRIGMAAIVIAVVSIIIASISLADFEIEIEEEEEEE
                     YRPMTREAFIFTQVDEHIDEEALAIPPDMFSQDTVVVKQGDRVKVHFYNLEPVESQEH
                     HTFTINDPAYKIHSDLNAQENTVIEFTASKSGVFDYICTYHQPTMRGELVVLSK"
     gene            complement(1751..2263)
                     /locus_tag="VGA92_03235"
     CDS             complement(1751..2263)
                     /locus_tag="VGA92_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845460.1"
                     /translation="MHFLSLDIIFIKPTFNQKECSASDCNYAFLCLDFVLSLPLQQGH
                     LVSCHSKNIEHWGHLDSTLIGDIVENVSSMIKNFSIIRYGKYDLSTTLDSLSMYIQMS
                     NSQMVHLKEGNTHRFLINHNLGMTWSLVLEELYKTVFREFVHDTHIQFKTTEDSLTAS
                     LVLESFQESD"
     gene            2258..2578
                     /locus_tag="VGA92_03240"
     CDS             2258..2578
                     /locus_tag="VGA92_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845461.1"
                     /translation="MQKQIQQKIAPWVWFTALGLTAFTVLALTTIVPIWHWVPISVTE
                     SATVVGITEQGCVVEGSYGYPITVSNCNAQPGETVQVSYNMPSIVQSEYMKRVQARAS
                     YVQP"
     gene            complement(2671..3678)
                     /locus_tag="VGA92_03245"
     CDS             complement(2671..3678)
                     /locus_tag="VGA92_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9845462.1"
                     /translation="MPPKKNNSAEEQNSHEHLVDTKITKKFLAFSPNETVEKIEELLD
                     IKASDFETIGYVYVIDQNGVLIGVVSLKHVLQSKPTTRLEEIMFKDVVSIKQHSHQER
                     VVSVALKHGLKAIPVVDNEKRLIGVIPHKTILSIFHHEFRKDLFQSGGILHAKEIESI
                     DTSVSKLVKARLPSLFLGLLGGLIVASIVTNFEEILNSYIILASFIPVIVYLTDAVGT
                     QSQTLVIRLLATEPNFPTARYIIREIKIGLFLGAVFAAMLFVAEILGWKQVELGIVIG
                     IAVFFSMVFQAFFSVYFSVTLAKFKKDPAIASGPIVTIISDITTIMVYFAVAIVVLEL
                     L"
     gene            4105..4932
                     /locus_tag="VGA92_03250"
     CDS             4105..4932
                     /locus_tag="VGA92_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845463.1"
                     /translation="MSTEKLAQKPISLPKDSSVSDVIRKLLDYNISRLVIKDHQRAVG
                     IITEKDVGFFLFNETTKHGLDKLPIDTIMNKITYVDEKEPPKNCAKIMLDKNISSVAL
                     GADDTIKGIITKTDLVKHYAENYEGRHKVSEHMTRDFVSTHSAAPLSKVIKKMLENKI
                     SRIIIKNQAERPTGIISFRDLFKISLALGSEEDDTGITISEKIRKGFLSEGGFGGISL
                     AGDVATKEIISIKFDADLVDACKLLLENKVSGLAVLDGNNEFSGILSKTDVTRALAS"
     gene            complement(5256..6647)
                     /locus_tag="VGA92_03255"
     CDS             complement(5256..6647)
                     /locus_tag="VGA92_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9845464.1"
                     /translation="MSSICKKRHLNWPSHGAEDVSRLQRLEEIGILKSRYDDNKNTVF
                     DLWYQWMRFSLKYNGALFEAASKSAMTFFNMGNAPLQKIRNAMRASFDSALRERLSKD
                     DVAASVARLVESQADFAKDGSNLLANYSDFISRISQLFEPFRDTMNRTPSDIIWFGGR
                     FCIHHYKSENEKRHKTPILVVYSLINRHYILDLLPDISIINNLLRQGFDVYATDWGTP
                     VSSDKDMSLENYAHEYVENAVDKIKEISGAEKVTLFGYCWGGIFALLYSCLHPESVKN
                     LILHATPTEIEGSETVIERWTAEINADKLVEAYGNVPGFFLNLAFVLRNPAETLLKYW
                     RYFSEPRSMSEILQFFSIETWLYDSVPIIGGVYREVIDQIYKENLLIKNKMKVDDKKI
                     DLNNITMPVLSVIGTEDDLVPSQSSKKIMDVVSSSDKKLIEFPTGHVGLCISQRAHEK
                     LWPEVGSWLASRS"
     gene            complement(6836..7168)
                     /locus_tag="VGA92_03260"
     CDS             complement(6836..7168)
                     /locus_tag="VGA92_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845465.1"
                     /translation="MSGNLPSLVKFFLQMRYDCNPQDKQNLESLQKQMQERINFLNEN
                     NLDTYGDPKLLESYELYAQIQECLFQDHTDQYILIAGIITGISLLIYVRWRMIKRAES
                     KKKLNFRG"
     gene            7335..9083
                     /locus_tag="VGA92_03265"
     CDS             7335..9083
                     /locus_tag="VGA92_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9845466.1"
                     /translation="MAEGEIIGAISGEVGQFHSVSQLIASSTSIQIAFAVLIIGIIVI
                     CTVYHKFSKWAQRKSLSYRRPHVSRLVQAAVLPFFAIALVSSVNVYIQVFELFVELSE
                     EELSAGAINPAETFAKILNTLNILVIGYTVAHIIPIILDKREKSILEWADFEAWKEKK
                     GFPDDEGDLFHKAYRWIPPTNTPEEMDEDEFKKHLQTEEGRKMLEKFRTSLGAPIGSY
                     QALIKNPFEEWKKSERAKYAKYYNACITGNNQSGRKLRPGQMPEEIYPIDVWREEKRL
                     NDYNEIIAGARPPGTAEKKRATVPKSFKQILPVGIFVAVIIGVVSWWGVDLVVLATAT
                     GGLAIGVGLALQETMQNYFAYVLIRKDKVFVEGDRIQLESGYNGYVHKITPRVTYVRH
                     PLNESFAIIPTRQLINAQITNFSKEIKLVPAIVNVGVSYLNDPKQVAAILIKVGKRGM
                     REIKDVKNKHMIVQQRCPYLDENMASCGCDRDVVDLDQPTVRFEKFNDSSLDFMMWVY
                     VRSYGNQFKVKSDLRIMIYEEFKKHDIRIPWPIRTIYQGDDKKEAEEIARLDEKRKKA
                     IEEFGIGDLLKGDSDE"
     gene            complement(9125..9382)
                     /locus_tag="VGA92_03270"
     CDS             complement(9125..9382)
                     /locus_tag="VGA92_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX9845467.1"
                     /translation="MPIYLDVHKVPFTEDNLKELCKLPRDEFGVRHINLFYNRESRVC
                     FCLLEAPNKEMVEDHHTKVNIKCEWIQEVVMAAPMSSPAPP"
     gene            9511..10557
                     /locus_tag="VGA92_03275"
     CDS             9511..10557
                     /locus_tag="VGA92_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845468.1"
                     /translation="MSPSDIDSTIHISDEITSSEITVLQVVSKICDRLDCCLDKRGPV
                     IQINDELLWNAVAQLKYKGTRLRLITEITKENLAYCKTIMRYFDVGHIDSVKGNFGIV
                     DGNEYLGNILPMDKDSQTNLIHINLRSFVETQQYLFDMLWSKAIPAKDKIKEIELGLD
                     KEFIETISDATEIKKLIFNLLRSATYEILVLFSSVNSFYRSEREGILDVLREAVERGV
                     NVRVLAPTEDDTIKDITHKKLVQRQKQIHIQYIRKPLQNKIITIIIDQILSLSIEIND
                     DMQKDFEKSTGTATYSNVDSTVSSCASIFESIWIQSELDKQNKIKQVYFQVFNGYELK
                     DETYERTWWNNKAK"
     gene            10600..10782
                     /locus_tag="VGA92_03280"
     CDS             10600..10782
                     /locus_tag="VGA92_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEX9845469.1"
                     /translation="MLKITWDDKICIHSGNCVKSLPSVFQVKDGKFVIIPNGAPEDQV
                     RKTVAACPSGALRILK"
     gene            10816..10992
                     /locus_tag="VGA92_03285"
     CDS             10816..10992
                     /locus_tag="VGA92_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845470.1"
                     /translation="MPNQTLQRACAMCGKMISEQKNQSLIEIDGITYAFDSKDCEIFF
                     KKFKSLYGEMFNPI"
     gene            11057..11896
                     /locus_tag="VGA92_03290"
     CDS             11057..11896
                     /locus_tag="VGA92_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9845471.1"
                     /translation="MTKCIAFHSYKGGTGKTTLACNLAVSLAKKGYRVFLLDLDVSAP
                     SIQAYFEKEPQKWINDFLNDSADISDVAIDMTTLLTESSGTSENKNGRFWVGFCNPKK
                     EEIYKLESGREDNAKMQLLRKFILLRERLISDYDVDYIIIDTSPGIRYWSINALAVAD
                     ILFLTLKMGELDVDGTKKMADDIYGSLTKYGAKSYLILNRVAGFCMPDSSYAHEHNLI
                     EQEIGFDEKLAKTVGMRIISAIPCYCDIQFSRKEFLTVIKHPTHPFASQINRLAEENE
                     IKV"
     gene            11974..12693
                     /locus_tag="VGA92_03295"
     CDS             11974..12693
                     /locus_tag="VGA92_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9845472.1"
                     /translation="MELDLAFDSEKCRETYRRLAGIYGHNISDTGLPDVINANFFFVD
                     VVGLSDPSLSVKNQMTKIDVLNRLIKTCSAFRSSKIKKIIMPTGDGMVITFLLNPELP
                     LNLSIELHQQIHQYNQDKAPNERIGVRIGLSSGPVFISNDINDNKNMWGPGIVIARRV
                     MDIGNESHILIADRLAEELMVLKDEYRTIIKPISDYTIKHDQTIKIYSAYSKDFGNPH
                     LPDKVIEFIERKNRNISKELS"
     gene            12885..13385
                     /locus_tag="VGA92_03300"
     CDS             12885..13385
                     /locus_tag="VGA92_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX9845473.1"
                     /translation="MSGETWSNLDKVDQKIIEILNVNARTPSKDIATELRKLGHDVSD
                     RTIRKRIERLEKSGIIKGYKAVLSDVTESNDHEALFVKFKVTKSLETLKEAIREHVVS
                     LPHYLLVANLEGEWNMLIIMRLERGLKNPAARIIERFSDDIIDYRITKFDFKNVNIVN
                     MSLLLL"
     gene            complement(13374..14123)
                     /locus_tag="VGA92_03305"
     CDS             complement(13374..14123)
                     /locus_tag="VGA92_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845474.1"
                     /translation="MATKKPDFDLALTHLRNNQNMAAYNMFTEIAESIKNSDPIKAAI
                     SYILASECRERQNKDVSSETTAAGSLFFSFAKKEKTYSSKTAFLCAAKCFLKAGRYDK
                     AKEAFEKSKDFKIEQIIDTRPIVIVDDSKAIVVKLQNLLGNLGYTNAHIFYNGNDALA
                     GCKKLLKSNPIILLDMGLPDINGDEVARKILAQKPDTQIILITADAKTTKRVKDTISS
                     GALAFIQKPFTIRELKDALSTADSEYSLSQK"
     gene            complement(14211..>15189)
                     /locus_tag="VGA92_03310"
     CDS             complement(14211..>15189)
                     /locus_tag="VGA92_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX9845475.1"
                     /translation="VGVILAALVFDFVNGWNDAANSVATVIGTKVLKPIHAVMLSAAA
                     NFIGPFVFGVAVATTIAKGIINPEDVTIYMIIGGLAGAISWASLATYFGLPISVSHSL
                     IGGLIGTGLAAVGYEKLLFDGLYKVLIGIIASPIGGLVIGLLLTTVIITIFAKYRPGP
                     VNKTFGRLQLFSSAWFALTHGANDGQKTMGIIVLVLMSEGILTEFEVPVWVILAAASA
                     IALGTFTGGFKVIKTLGMKIARLRPYQGFAAETGGGIVLAIFASLGIPASTTHAITGS
                     IMGAGATRRKQAVRWKIGRQIVFAWLITIPGSAAVAFVATHIIYFLVGT"
BASE COUNT         4600 a         3047 c         3042 g         4500 t
ORIGIN      
        1 tattattttt ctgaacacat tacaagtaaa aatctggcct gttccaaatg gcgtcttgtg
       61 ttcttcttca aaacttttga tgtgttcgaa tccattcgaa aataactgtt tcatagattc
      121 ttcggaaaat tgttttgtgt ctagtccgct acacatcatt gggccatttg tagaaaaagt
      181 agagagaatc agatagccgc ctttctttac gtgttttctg gttaattcca cataattttt
      241 tagatcctct tcagaggtta gaaagtgaag tagtgctcta tcatgccata tatcatatct
      301 gtcgtttgta tcaaactctg ttatatcgca ctcaatccat tttaccattt cagctctctc
      361 tcctagtctt tcttttgctc tttccaatgc ttttgcagaa atatccagta ctgtaatatc
      421 actaaaatct aggtcaagta gtttaaccac aaggtttgag cctcctccac ccacatcaac
      481 aattctagcg tccttactgg gtttagttga caatatcaaa tcaattgacg ttttgggatc
      541 atgctgatac caactgacct cgttggattt ttttctagtc caaacatttt cccaatgttc
      601 tttcatatca tacatcttta atcatgtttt tttattcaaa ttctctaata atgatttgtt
      661 gaataacctg cttattacag gcatgttttg aacgggacgt gcaaaatcgt gattcttaac
      721 tattatctcc cccaaaaaaa tacactggca atcaaatttg aaccacctaa tccgtgaagg
      781 gtttgaacct tttgaccatt tgcttcgctt gagacacatt attttttatt tcaaacaagt
      841 cattaaaaac tagaataatt ttgaatagaa tgcgttagtt tattgaatta atttgaaatt
      901 gaacttttct catagagatc gtgtttttct tcgttgccca gagcctcaat aagatcctca
      961 tgatcagtca caaattgttc aataaatctc tcatcagatt ggttcatgtc ttactaacca
     1021 gttttgtaac atataagatt attttggcaa ttatctttcg gttaatcgcg ataacaacaa
     1081 ctcgaagtta taatttatgg caagtgcact atttcggttc caaagttatt tggataggac
     1141 tactaactct cccctcatag tgggttgatg atacgtgcaa atgtagtcaa atacgccgct
     1201 ttttgaagct gtgaattcaa tgacggtgtt ttcttgtgcg ttaagatcac tatgaatttt
     1261 gtatgccgga tcattaattg taaaggtatg gtgttcttga gattctaccg gctccaagtt
     1321 gtaaaaatgc accttgacac gatcgccctg ttttacaact actgtatctt gtgaaaacat
     1381 gtctggagga atcgctaacg cttcttcgtc tatgtgttca tcaacctgtg taaagataaa
     1441 ggcctctctg gtcataggac ggtattcttc ctcctcttct tcctcgattt ctatttcaaa
     1501 atcagccagt gagatacttg caattattat agagaccaca gcaatgacaa tggccgccat
     1561 tccaattcta agattctttg aatctgctac actcaacgga atagtacagc tgagcctgcc
     1621 tattaataag aaagatgatt ttcaatcttg gttttcacaa ttatgtgatg ttgccacaat
     1681 atctaaagta cgaaaatttc ttttttgtag gatactatct agtcggtaag atctacaaac
     1741 tgatcaaatt tcagtcgctt tcttgaaatg actctagtac caatgatgca gttagagagt
     1801 cttcggttgt cttaaattgg atgtgagtgt cgtgaacaaa ttccctaaac accgtcttgt
     1861 acagttcttc taaaacaagc gaccatgtca ttcccaaatt atggttgatt agaaatctgt
     1921 gagtgtttcc ttcctttagg tgaaccatct gagagtttga catctgtatg tacattgata
     1981 atgaatcaag agttgtgctc agatcatatt ttccatacct tatgatgctg aaatttttga
     2041 tcatagaaga gacattctct acaatatcgc ctatcaacgt gctatcaaga tgcccccagt
     2101 gttctatgtt tttagaatgg caggaaacca gatgtccttg ctgtaatggt agactgagga
     2161 caaagtccag acacaaaaac gcataattgc aatcgcttgc agagcattcc ttttgattaa
     2221 atgtgggttt gatgaatatt atatcgaggg aaagaaaatg caaaaacaaa ttcagcaaaa
     2281 gatagcacca tgggtatggt ttacggcact aggattaacg gcatttaccg ttcttgctct
     2341 gactaccatc gtcccaatat ggcactgggt gccaatttct gtcacagaaa gtgcgacagt
     2401 agtaggcatc acagaacaag gatgtgtagt tgaaggaagc tacggttatc caataactgt
     2461 aagcaactgt aatgcccagc ctggcgagac agtccaagtt tcatacaaca tgccttccat
     2521 agtgcaaagc gaatacatga aaagagttca ggcaagagca tcgtacgttc agccctgagc
     2581 ttgatttttt tctagttttt acttttgatt atcaattttg atattatgtt ggatttctga
     2641 gaatggtttt tcctaaaaga aacaatcagg tcataataat tccagtacca ctatggctac
     2701 tgcaaaatac accattattg tcgttatgtc gcttattatg gtcactatcg gccccgaggc
     2761 aattgctggg tcttttttga attttgcaag agtaactgaa aaatatactg aaaaaaatgc
     2821 ctgaaaaacc atgctgaaaa atactgcaat tccaatcaca atgcccaatt ctacttgttt
     2881 ccagccaaga atctccgcaa caaacaacat tgccgcaaaa acagccccca aaaatagccc
     2941 gattttgatt tcgcgtatta tgtatcgtgc agtcggaaaa ttcggctccg ttgccagtaa
     3001 tcttatcacg agtgtttgtg attgggtccc tactgcgtct gtcagataca ctatcaccgg
     3061 aatgaatgaa gccaaaatta tgtaagagtt taggatctcc tcaaaatttg taacaattga
     3121 tgctacaatc agacccccaa gaaggcccaa aaataaagac gggagtcttg ccttaactag
     3181 cttcgagaca gatgtgtcga tagactcaat ttcctttgcg tgtagaattc ctcctgattg
     3241 aaacaaatct tttctaaact cgtgatgaaa tatcgagagg attgtcttat gtgggatcac
     3301 gccgattagc cgtttttcat tatctactac aggtattgcc tttaagccat gttttaatgc
     3361 aacagagacg actcgttctt ggtgagagtg ttgtttaatt gagacaacgt ccttgaacat
     3421 tatctcttct agccttgttg taggtttgct ctgaagaaca tgctttagtg aaactacgcc
     3481 aattagaaca ccgttttgat ctataacata aacatagcct attgtttcaa aatcacttgc
     3541 tttgatatct aataattcct cgattttttc aaccgtttca tttggggaaa acgccaagaa
     3601 tttttttgtt atctttgtgt ccactaaatg ctcatgagag ttttgctctt ctgcactgtt
     3661 gttcttttta ggtggcatat agcctaaaaa ggcagatcag ctattaaatg gcttatcttc
     3721 attttttcca catctggcta atcattaaag cgcaaaaatt tctcttaatg taagaataaa
     3781 aaaagagttc aactattgct tgcccaagct agaggaaaat tggttgatca atcaaatcaa
     3841 ttgatcttag gatccgccca tgcccagtga actgttatcc actagattaa acgtctatct
     3901 tgttttagct gtttgatggt gttttggtta gttttgtgag tttgaaacgc ttaacataag
     3961 gtgtaattgg ttattttgcc tatctgtttt gactcaaaat ctaaaattca taaaccatca
     4021 gatcgtatat caaaagcatt ttttgatagt cagagttgaa aattatcgct ttgtctttta
     4081 tagtaaactg aggtggcatt agtattgtct acagaaaaat tagcccaaaa accaatctca
     4141 ctccctaaag attcgtcggt atctgatgtg atacggaaac ttttagacta taacataagc
     4201 agattggtca tcaaagacca ccagagggct gttggcataa tcaccgaaaa agacgtcgga
     4261 ttctttttgt ttaatgaaac aacaaagcat ggcctagaca agctgccaat agacacaatc
     4321 atgaataaaa ttacatatgt tgacgaaaag gaacctccca aaaactgtgc gaagatcatg
     4381 ctggacaaaa atataagctc cgttgcgtta ggagccgatg acacaattaa gggaatcatt
     4441 acaaaaacag atctggtaaa acattacgca gaaaattatg agggtcgaca caaagtatca
     4501 gagcatatga ctcgcgactt tgtctcaact cattctgcag cgcccctatc aaaggtaata
     4561 aaaaaaatgc ttgagaacaa gatctcaaga ataatcataa aaaatcaagc cgagagacca
     4621 accggcatca tctcatttag ggatcttttt aaaatttcat tagcacttgg aagtgaagag
     4681 gatgacaccg gtattaccat atctgagaaa ataaggaaag gatttttgtc agaggggggg
     4741 tttggaggca tttcattggc aggtgatgtt gcaacaaaag aaataatctc aataaaattt
     4801 gatgcagatc ttgtcgatgc atgtaagctg ctgcttgaaa acaaggtcag tgggcttgcc
     4861 gttctggacg ggaataacga gttttcagga attctcagca agacggatgt tacaagggct
     4921 ttggcatcct aaatatggtt catttttgca atttgtaaat ttagtcatag tctttagtaa
     4981 atcactccaa actttggtag tacaaagcaa tcgatgtgac ttggataaaa ccacattacc
     5041 ggttcttgca cctcccaggg gaataaaacc aaatcaattt gaattagaaa tttttgtgaa
     5101 tttgaaatag caaaaattgc atccaaaaaa tcaccgctta caaacacaaa gatgtcaaaa
     5161 tcagaataat ttgagaactg ccaaggccta atctggtgtt cttaattgtc agtatggaat
     5221 actcaattat ttttgacagt aatcttttgt atagattagg atcggcttgc caaccagctt
     5281 ccaacttctg gccaaagttt ctcatgggcc ctctggctga tgcaaagccc cacatggccc
     5341 gtcggaaact cgattagctt cttgtcggag cttgaaacaa catccatgat ttttttagat
     5401 gactgagacg gaaccaaatc atcttctgtt ccaatgacgc taagaacagg catcgtaatg
     5461 ttgtttaggt caattttttt gtcgtcaacc ttcattttgt ttttaatgag caggttttct
     5521 ttatagatct ggtcgattac ctccctgtac accccaccga ttatcggaac gctgtcataa
     5581 agccatgttt ctattgagaa aaactgcagt atctcgctca tgcttcgtgg ttcgctaaaa
     5641 tacctccaat actttagcaa cgtttcagca ggatttcgaa gaacaaacgc caagttgagg
     5701 aaaaatcctg gaacgtttcc ataagcttcg acaagtttgt ctgcgtttat ctcagctgtc
     5761 cacctctcaa ttaccgtctc gcttccctca atctcagtag gggttgcgtg aagaatcagg
     5821 ttttttacac tttctgggtg caggcatgaa tacaggaggg caaaaattcc gccccaacaa
     5881 tatccaaaca aagtcacctt ttctgcgccg gaaatttcct tgattttatc tactgcattt
     5941 tctacatatt catgcgcata gttttcaagt gacatgtcct tgtctgagga gacgggggtt
     6001 ccccagtctg tggcatatac atcaaagccc tgccgcagca gattgttaat tatgctaatg
     6061 tctggaagca agtcaagaat gtagtgtctg ttaataagag agtatacaac aaggattggt
     6121 gttttgtgcc gtttctcatt ttctgatttg tagtggtgaa tgcaaaatcg tccaccaaac
     6181 cagataatat cggaaggagt cctgttcatc gtgtccctga atggctcaaa cagttgtgat
     6241 attcttgaaa taaaatcaga ataattggca agaagattag acccatcctt tgcaaagtct
     6301 gcctgggatt ctaccagtct tgcaacgctt gcggccacat catctttgct tagacgctcc
     6361 cttaaggcag agtcaaaaga tgcgcgcatt gcgtttctaa tcttttgtaa tggcgcgttg
     6421 cccatattaa aaaaagtcat ggcagatttt gaggctgcct caaaaagtgc gccgttgtac
     6481 tttaaagaaa aacgcatcca ctggtaccac aaatcaaaaa cggtgttttt gttatcatcg
     6541 tatcggcttt ttagaattcc aatctcttct agtctttgca acctagaaac atcctctgcg
     6601 ccgtggcttg gccaattcag atgccttttt ttgcagatac tgctcaacgc aaatatcgga
     6661 gcaaaaatat tttccgaatc ttttgattgc cgtttccttc tttacatcca ttccgcagat
     6721 tgggcagtgt tttgaaaacc caaacatggt agtatgtccg ccttatgata tttaatagga
     6781 agaggccatt ttcaggactg ataataaaac actaccacta gacacaactt tgcgatcatc
     6841 ccctaaaatt cagcttcttt ttagattctg ctctctttat catccgccag cgaacgtaga
     6901 taagaagaga aatcccagta atgattccgg ctatcagtat gtactggtct gtatgatcct
     6961 gaaagagaca ttcttggatc tgggcgtata attcgtagct ttccaaaagt ttgggatcgc
     7021 cgtatgtgtc caagttattc tcattaagaa aattgattcg ctcctgcatt tgtttttgca
     7081 gcgattcgag gttttgcttg tcttgtgggt tacagtcata ccgcatttgt aagaagaatt
     7141 ttacaaggct tggcaaatta ccagacatta ggcattcttt gcatttagta ttattgattt
     7201 aattctgttt attttgatgc cattcacaaa ttggattgag ctgagatttt tttcatccca
     7261 cgccacccca actatcgcat tttctgcaac aaaactaact tatgtttgta aatttatgat
     7321 ttttacagga gaatatggcc gaaggagaga tcataggagc aatatcaggc gaggttggac
     7381 aattccattc tgtttcccag ctcatagcgt cttcgacgtc cattcagatt gcatttgctg
     7441 ttttaattat tggcatcatt gtaatttgta ccgtatacca caagttctcc aagtgggccc
     7501 agcgcaaaag tcttagctat cgcagacccc acgtatctcg cctagtacag gccgcagtgt
     7561 tgccgttttt tgccattgct ctggtatcat cagtcaatgt ctacatacag gtttttgaat
     7621 tgtttgtcga gctctcagaa gaggagctgt ctgctggcgc gattaacccc gctgagacgt
     7681 ttgccaagat tttaaacacg ctaaacattc tggtcattgg ctacaccgta gctcacataa
     7741 taccaataat acttgacaag cgcgaaaaat caattctaga gtgggcggat tttgaagcct
     7801 ggaaagaaaa aaaaggattt ccagacgacg agggagactt gtttcacaag gcatacaggt
     7861 ggataccccc gacaaataca ccagaagaaa tggacgaaga tgaattcaaa aagcatctgc
     7921 aaacagaaga ggggcgaaag atgcttgaaa aatttagaac gtcgcttggc gcacccattg
     7981 ggagctacca ggctctcatc aagaatcctt ttgaagaatg gaaaaaatca gagagggcaa
     8041 agtatgcaaa atattacaac gcttgcataa caggcaataa ccaatctgga aggaagctta
     8101 ggccgggcca gatgccagaa gaaatctacc caattgatgt gtggagagaa gaaaaaaggc
     8161 taaatgacta taatgaaatc atagcaggtg caagaccccc cggcactgca gaaaaaaaga
     8221 gggcaacagt tccaaaatcc ttcaaacaga ttcttccagt aggaattttt gttgcggtga
     8281 taattggcgt agtttcctgg tggggcgtag acctagtggt gctggcgact gccactggcg
     8341 gcttggcaat aggcgtcggc cttgcattgc aagaaaccat gcaaaattac tttgcctacg
     8401 tcctaattag aaaggacaag gtcttcgttg aaggcgacag aattcagcta gagtcaggct
     8461 ataacggata cgtgcacaaa atcacgccgc gagttaccta cgtaaggcat ccactcaacg
     8521 agtcttttgc aatcattcca actaggcagc tgataaatgc ccagatcaca aatttttcaa
     8581 aagagatcaa gctggtacct gccattgtaa atgtcggcgt atcatatctt aacgacccaa
     8641 aacaagttgc cgccattttg ataaaggtgg gaaaacgcgg catgagggaa attaaggatg
     8701 taaagaacaa gcatatgatc gtacagcagc gttgccccta cctggacgaa aacatggcaa
     8761 gctgcggctg cgatagggac gttgttgatt tggatcagcc gacggtacgg tttgagaaat
     8821 tcaacgattc atcccttgat tttatgatgt gggtatacgt tagaagctat ggcaaccaat
     8881 tcaaggtcaa aagcgaccta cggattatga tttacgaaga attcaaaaag cacgacatac
     8941 gaatcccgtg gcccatcagg accatctatc agggcgacga taaaaaagaa gcagaagaga
     9001 ttgcaaggct tgatgagaaa agaaagaagg caatagagga atttggaata ggcgatttgc
     9061 ttaaaggcga ctcagacgag taaaactaac gtacttgttt ttcagtaatt atttagacta
     9121 gcgattaagg cggtgctggc gatgacatcg gcgccgccat tacaacttcc tgtatccatt
     9181 cacatttgat gttgacttta gtatggtggt cttcaaccat ttccttgttt ggcgcctcaa
     9241 gcaaacaaaa acaaactctt gactccctat tgtagaacaa attgatgtgc cttactccaa
     9301 attcgtcacg cggtaatttg caaagctctt tcaaattgtc ctctgtaaaa gggactttgt
     9361 gaacgtcaag atatattggc atgattccat gtcagtaacc taatttatat aatttttcca
     9421 agtaacttca agttttttta tgaaaaaatc aagttttagt aaaatatgat ctgctgtact
     9481 tatattcata aattcactac acattatgat atgagtccat cagatattga ttcaaccata
     9541 cacatatctg acgaaatcac tagctccgaa atcactgtcc tacaagtagt ttcaaaaatc
     9601 tgtgacaggt tggattgctg tttggacaag agaggtccag tgattcagat aaatgacgag
     9661 ttgctttgga atgcagttgc gcaattaaaa tacaaaggca ccagattgcg actgatcact
     9721 gagattacca aagaaaacct cgcatactgt aaaaccataa tgaggtattt tgatgtggga
     9781 cacatcgact cagtgaaagg taattttggg attgtggatg ggaatgagta tctgggcaac
     9841 atactcccaa tggacaaaga ctcacaaact aatctaatac acatcaacct gaggtcattt
     9901 gttgaaacgc agcagtatct ttttgacatg ttatggagca aagctattcc ggcaaaagac
     9961 aaaatcaaag agatagagct gggcctcgat aaggagttta tagaaacgat cagtgacgcc
    10021 acagagatta agaaattgat cttcaacttg cttcgttccg ccacgtatga aatactggtg
    10081 ttattttcct cagttaactc attttatcgc tcggagcgag aaggtatatt ggacgtatta
    10141 agagaagcag tcgagcgagg cgtaaacgtc agggttttgg ctccaactga agacgacacc
    10201 ataaaagaca ttacacacaa gaaattggtg caaagacaaa aacagatcca catacagtac
    10261 attcgaaaac cactgcaaaa taagataatt acgataatta tcgatcaaat attgtcgctt
    10321 tcgattgaaa ttaacgacga catgcaaaaa gattttgaaa aatccacagg aactgcaaca
    10381 tattctaatg ttgactctac tgtctcttca tgtgcatcaa tattcgaaag catatggata
    10441 caatcagagt tggataagca aaacaaaatc aagcaagtct attttcaagt gtttaatggt
    10501 tacgagttaa aagatgaaac ctatgagcgc acatggtgga ataataaagc caaatgatct
    10561 tttactctac tttatatgtc atgattagag atgtctggca tgctaaaaat aacatgggat
    10621 gataaaattt gtatacattc tggcaactgc gttaagagcc tcccatcggt atttcaggta
    10681 aaggacggaa agtttgtgat catacctaat ggagcacctg aggatcaggt cagaaaaacg
    10741 gtggctgcct gcccatccgg agcattgagg atcctcaaat aaggcccagg cattactgac
    10801 aactaaggaa gaaaaatgcc aaatcaaacc ttgcaacgcg cctgtgccat gtgtggaaag
    10861 atgatttctg agcaaaaaaa ccaatctcta atcgaaattg atggtatcac ctatgctttt
    10921 gacagcaagg attgcgagat attttttaag aaattcaaaa gcttgtatgg tgagatgttc
    10981 aacccaattt gagttttaaa cttgctagaa aacaaaatca gaaatttcta aatatgcacg
    11041 gtttcattta aaaaagatga ccaagtgcat cgcgtttcat tcttacaaag gaggcactgg
    11101 caaaacaact cttgcgtgca accttgcagt atcgttagct aagaaaggat atcgcgtctt
    11161 tcttcttgat cttgatgttt ctgcccccag cattcaagct tattttgaaa aagaaccaca
    11221 aaaatggatt aatgattttc taaatgacag tgcagatatc agcgatgtcg caatcgacat
    11281 gacgacgctt cttactgaat cttctggcac gagtgaaaat aagaatggca ggttttgggt
    11341 gggtttttgc aatccaaaaa aggaggaaat ctacaaactt gagagcggca gggaagataa
    11401 tgcaaagatg cagttgctca gaaagttcat ccttctaaga gaacgactta tttccgatta
    11461 tgatgtagac tacatcataa ttgacaccag ccccggcata agatactggt caattaacgc
    11521 gttggcagta gctgatattt tatttttgac acttaaaatg ggagaactag acgttgatgg
    11581 aacaaaaaag atggctgacg atatttatgg ttcacttaca aaatatggtg caaagtcata
    11641 tttgatatta aaccgcgtgg ctggattttg catgccggat tccagttatg cacatgagca
    11701 taatctgatt gagcaggaga taggctttga tgaaaaactc gcaaaaactg tgggaatgag
    11761 gatcatttct gccattcctt gttactgtga cattcagttt agcagaaaag aattcttgac
    11821 tgtcataaag catccaactc atccttttgc aagccaaata aatcgccttg cagaagagaa
    11881 cgaaatcaaa gtttaagtct tgttttcatt catgaactaa cgttgtcaga tgtttgtacc
    11941 ttatgtggaa agcaaatttc cggttctcca gtaatggagt tagatctggc ctttgatagc
    12001 gaaaaatgca gagagacata cagaaggcta gctgggatat acggacacaa tatttcggac
    12061 acgggtttgc cagatgtcat caatgcaaat tttttctttg tcgatgttgt aggactatcc
    12121 gatccctcac tttcagtcaa aaaccaaatg acaaagatag atgtcctcaa cagattgatt
    12181 aaaacatgtt cggcctttcg ctcgtcaaag ataaaaaaga tcatcatgcc aactggcgac
    12241 ggaatggtaa tcacgttttt actcaaccca gagttgcccc taaatctaag catcgagtta
    12301 catcaacaaa ttcatcaata caatcaggac aaagcaccca atgaaagaat aggtgtccgg
    12361 ataggtctta gctcaggacc agtctttatt tcaaatgaca ttaatgataa caaaaacatg
    12421 tggggcccag gtattgtaat agctagacgt gtgatggata ttggaaatga atctcacatt
    12481 ttgattgcag acaggttagc tgaagagtta atggttttaa aagacgaata caggacaata
    12541 atcaagccaa tatctgatta taccataaaa cacgatcaga ccattaagat ctattcagcg
    12601 tattctaagg actttggaaa tccccatttg ccagataaag tgattgaatt cattgaaaga
    12661 aagaatagga atatcagcaa agaattatcc taattggtac caagaatcgg tttttgaaaa
    12721 aacttgttga aacccaacaa gtttgattga atttagcggg gtttgtgagg tttggatctt
    12781 ttgactgatt cttttggaga ccatacactt tgcaatcaca aacaagaaag tccatttttt
    12841 gcactacata ttattcaaaa gccacgcagt ttttccacat tgtgatgagc ggagagactt
    12901 ggtccaacct agacaaagta gaccagaaaa taatcgagat cctaaacgta aatgcgcgca
    12961 ccccctcaaa ggacattgcc actgagctaa gaaagcttgg acacgacgtc tcagacagaa
    13021 ccatcagaaa gagaattgag cgcctagaaa aaagcggcat tatcaaaggc tacaaggcag
    13081 tgctatccga cgtaacggaa tcaaatgatc acgaggcgtt gtttgtaaag ttcaaggtaa
    13141 ccaagtctct agagacccta aaggaggcaa tacgcgaaca tgtggtttct ctgccccact
    13201 atctcctagt agcaaacctg gaaggcgaat ggaatatgct gataattatg cgccttgagc
    13261 ggggactaaa gaatcccgcg gcaagaatca ttgaaagatt ctcagatgac atcatagact
    13321 acaggataac caagtttgat ttcaaaaacg taaatatcgt aaacatgtca ctgctacttt
    13381 tgtgatagtg aatattcaga atctgcagta gagagcgcat cctttagctc ccggattgta
    13441 aatggttttt ggatgaatgc tagtgcgcca gacgatatgg tgtctttgac tcgttttgtt
    13501 gtttttgcgt ctgcagtgat tagtatgatt tgcgtgtctg gcttttgtgc caagattttt
    13561 cttgccacct cgtcgccgtt tatgtctggc agccccatgt caagcagaat tattggattt
    13621 gattttagca gtttcttgca gccagctagt gcgtcgttgc cgttgtaaaa tatgtgtgca
    13681 tttgtgtagc caaggttgcc aagaaggttt tggagtttta ctacgattgc cttgctgtca
    13741 tccactataa cgattggccg tgtgtctatg atttgctcta ttttgaagtc ctttgatttc
    13801 tcaaaagctt cttttgcctt gtcgtatctt ccggctttaa gaaaacattt tgctgcgcac
    13861 aaaaacgcag tctttgatga atacgttttc tccttttttg caaagctaaa aaacagactt
    13921 cctgcagccg ttgtctcaga tgatacgtcc ttgttttgtc tctccctgca ctcggatgca
    13981 agtatgtacg agattgccgc cttgattggg tctgagtttt ttattgattc tgcaatttct
    14041 gtgaacatgt tgtaggctgc catgttttgg ttgtttcgaa ggtgtgttag cgccaagtca
    14101 aaatcaggct tttttgttgc catgattttt gatagtgtcc gtgttttatt atattagctg
    14161 atatccaaaa aaacacactc tggatcaaaa atttgacatt tccgtttcta tcaagtgcca
    14221 accaaaaagt aaatgatgtg cgttgcaaca aatgccactg ccgcactgcc aggtattgtg
    14281 attagccatg caaacacaat ttgtcttcct atcttccagc gcacggcttg ttttcttcgc
    14341 gttgccccag cccccattat ggatccggtt attgcgtgtg tggtgcttgc cgggatccca
    14401 agtgatgcaa agattgccag aactatcccg cctcccgttt ctgctgcaaa tccctggtat
    14461 ggccgcagtc ttgcgatttt catccccaaa gtctttatca ccttaaagcc cccagtgaag
    14521 gtgccaagcg caattgcaga cgctgctgcc aaaatcaccc aaactggaac ttcgaattcc
    14581 gtcagtattc cctccgacat tagaactagt acaattatcc ccatcgtctt ttgtccgtcg
    14641 tttgcgccgt gcgtcagagc aaaccacgca gatgaaaaca gttgcagcct tccaaatgtt
    14701 ttgttcacag gccctggtcg gtattttgca aatattgtga tgatgacagt ggtaagtagc
    14761 aagccaatta ctagccctcc aatcggggag gcaattattc caattagcac cttgtagagg
    14821 ccatcaaata gcaatttttc atatccaact gccgcaagtc cggttccgat cagtccgcca
    14881 atgagtgaat ggctcaccga gattggaagt ccaaaataag tggcaagcga agcccatgag
    14941 attgcacctg ccaggccacc aattatcatg taaattgtga cgtcttctgg gtttatgatc
    15001 ccctttgcaa ttgttgtggc aactgcgacg ccaaagacaa atggtccgat aaagttcgca
    15061 gctgcagaaa gcatcactgc atgtattggt tttagaacct tggtgccaat tactgtggca
    15121 acggagtttg ccgcatcgtt ccagccgttg acaaaatcaa aaactagcgc tgccagtatt
    15181 acccctact
//
LOCUS       DASWKH010000044        13995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_830332, whole genome shotgun sequence.
ACCESSION   DASWKH010000044 DASWKH010000000
VERSION     DASWKH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 13995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13995
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830332"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..790)
                     /locus_tag="VGA92_03315"
     CDS             complement(191..790)
                     /locus_tag="VGA92_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEX9845476.1"
                     /translation="MKKLQLEQVLASKKQVKIRSKKDNVRIARTRRSRGYHWEDTLVK
                     RFNALEGWKAFRLGSPSVGLPDILVVSTKNCTIFTIEAKSGTTNSLVVPFDQILRCLR
                     WTDTFELYDTRKVVFAFKFLSKKRIGLSLYEKRELREYYKVWDKENMISDIACNYDGS
                     TYSIIEGKRQKLTLDDYQMPFVSRNAKTFNQNANTDNFE"
     gene            complement(854..1186)
                     /locus_tag="VGA92_03320"
     CDS             complement(854..1186)
                     /locus_tag="VGA92_03320"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9845477.1"
                     /translation="MSVFESELKKGRFVVGECSKCQKVTWPPNDFCSHCFGSLSWRGI
                     KEPGIIVECSSKDGRTFCIVEFENTVRVMGTISGNTEPKPGAQIRVISCSFDDSPKFT
                     FGPEKSDL"
     gene            complement(1183..2286)
                     /locus_tag="VGA92_03325"
     CDS             complement(1183..2286)
                     /locus_tag="VGA92_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9845478.1"
                     /translation="MKKIAVASYCTTKFSVDDTPIESLLFSATKLVFEQTKNLDQKDV
                     DAVLVSTNENKKYLSSIVAEMSGITPKISHSVESLCNSGTNSVISAYSYIQAGLADVA
                     LVVGAERFDSPGQVLDWDVSRGEFKHPIFWASMFTRAHKRKFGTTDEQLAYVSAKNHK
                     NAQDNPAAYPKHHSMDEIMHSKRLTDDLRLLDCSRPCTGSSAVLLASEDVVRKFTDSP
                     VWITGIGQKTTSASFTKNDLVTLQSTADAVAQAYTMSKIEPSQIDVAEIHDAFSICEL
                     MILEDLEIVRSGQAGKYVADLYNTNSKKINPRGGLIGSGHPLGATGVAQVAEIACQLE
                     GNAKKRQVDGAKIGLVQNMSAAATSSTVLVMRS"
     gene            complement(2283..3248)
                     /locus_tag="VGA92_03330"
     CDS             complement(2283..3248)
                     /locus_tag="VGA92_03330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9845479.1"
                     /translation="MKLSYSLGSLLSISDLLICAEKISKNRPDTVWVPETWGMENFAM
                     LSAVCQKTPSKIGSSIINIFSRSPTLIAMGAATIDTLSNQRLVLGLGTSSIPIVEGLH
                     GYKFSRPLKRMQEYVEIIRLTLSGKKIDYSGEIFSLNGFTLLIKPPRQNIPIYIAAIN
                     QKMIELTWKIADGVIFYLRPISEMKNTIAKMQSKRTIDVACQFITCVSEDEQLAKDRA
                     RKTLAFYISVGDIYRRFLAENGFKKETDSIYSQYLKSGLKSNHESVTDAMLESLAICG
                     TPEQCKEQLRRTYDAGITHPIIQFNPIGDVQASFDLLTKTFSDIP"
     gene            complement(3258..3947)
                     /locus_tag="VGA92_03335"
     CDS             complement(3258..3947)
                     /locus_tag="VGA92_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9845480.1"
                     /translation="MQAVILAGGKGTRLKPITDYVPKSLVPLNNIPIIEWQIRYLKKF
                     GVRDFVICSGYKTDQLENYLAHKQNFGCKIKYSTEKKPLGTGGAIKNAAKLIRGKSFL
                     VLNGDVITTIDVRKLFKKPNSIALVELRTKFGTVDLSGDVIVDFREKKSISNVWMNAG
                     IYHLEKKTLEDIPSNGSIEEITFHKYAKAKKLSGIKFKDTFWFSIDSHKDLEECATIM
                     KNKKYEKFVSS"
     gene            complement(3996..4292)
                     /locus_tag="VGA92_03340"
     CDS             complement(3996..4292)
                     /locus_tag="VGA92_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845481.1"
                     /translation="MKTRYKIIGILALAFGLINIFWYTPYSLALVFMNQPFHFQHLQN
                     IEVYDGGGIGTQILYLPFEAIQSDPYWLIWSLSLYFGIVILSVLGVGSLRQKHE"
     gene            complement(4516..4986)
                     /locus_tag="VGA92_03345"
     CDS             complement(4516..4986)
                     /locus_tag="VGA92_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845482.1"
                     /translation="MSKIVVLLGIFALFGMFAIFSNFAFASCAVNEDWSDAPCFDVLP
                     VNREEYRAAWAPYYDYKGSDWMEQKKAEMFDAKDEGMLAEWMDGSSQNHNVFSYYQSR
                     GEISFPLEYDRPFFEDDPRYYLQFFTQGWLFFVLIGVSAGLIAGAAVFIIRRRK"
     gene            complement(5473..6147)
                     /locus_tag="VGA92_03350"
     CDS             complement(5473..6147)
                     /locus_tag="VGA92_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845483.1"
                     /translation="MSITATPVFAERAVEVTKEIDVRKEVLAIVLTDLRSYKEIFPTL
                     VKDVQISSETTQAKFIIIAQGTHDADVRSSILQDGSFAIDIISGDLKGSQIITNLEKR
                     VGFDGTPDGATIVKATLTLKTSWWVSAALSFVSDSDIEKAVGDGFYELGQYAKSGYSQ
                     GELVNMDYEEKTSSQELQVSTEPKEKDTIQKLQVFKIESEEKPKNTAFVKTERSFGRN
                     SVFLST"
     gene            complement(6277..6804)
                     /locus_tag="VGA92_03355"
     CDS             complement(6277..6804)
                     /locus_tag="VGA92_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9845484.1"
                     /translation="MLEIQPFIQWISSLISDYLYAGVFAAAVIETVFPPIPTMAVFPF
                     AGYIASQNGMSLPEAIGLGMAGGLGATIGSTIIYVICIKLGRVALLRYLKYAKISEER
                     LAKIEKWFEKHGDKAVFLGRLVPVLREMISIPAGLLGMKPIKFLLYTFGGSCVYGVAL
                     TLVGYYFGLVTIDLF"
     gene            complement(6926..7306)
                     /locus_tag="VGA92_03360"
     CDS             complement(6926..7306)
                     /locus_tag="VGA92_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845485.1"
                     /translation="MALKAALIITGIAIGLLAIYYADVGVAAGADYGFLPFDHKTRGM
                     VLGGPAMALPIIGFFISRNEPSRPLAAMLFVVGIMIIVGGAYILANAPAETLERNPMA
                     EAAPLFGVGAFQIALGAIKIRKSK"
     gene            7453..7812
                     /locus_tag="VGA92_03365"
     CDS             7453..7812
                     /locus_tag="VGA92_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9845486.1"
                     /translation="MSTDVLHEIAIDEDDKKQKALEVFSDNYTRIILATMMEKPRSAL
                     QIAEETKIPLTTVYRKLHHLLENNMIRISGQIAENGKKNFLYKSKIKSFHITFTKDKL
                     AILVNTSGTCKFCLQAH"
     gene            complement(7837..8532)
                     /locus_tag="VGA92_03370"
     CDS             complement(7837..8532)
                     /locus_tag="VGA92_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9845487.1"
                     /translation="MLELAALDQIRIILAVSMLGIATASDIKKREINDIIWVVFGAIG
                     AILIPFAGDLNDELLKIGISMIVAPIAIIIWRFGLFGGADAFALIALAVLAPNMTLVE
                     GTITPFTVLTNAVLLSVVPMFINAVRNVILIARKIDIFEGFDETKGRKLLAMFIGYRA
                     ANPKFGFSIERKNGKRKKLNLSFQHAEYAAFCEKKDTWITPGVPYMIFIFAGFVIQLI
                     YGDIIFSLFSGLS"
     gene            8691..8870
                     /locus_tag="VGA92_03375"
     CDS             8691..8870
                     /locus_tag="VGA92_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845488.1"
                     /translation="MNSYLLPCKAETGLSSDRLPQELRECTPVEIRSSVVEAGRCSCG
                     DMIALPHNWGIIRVI"
     gene            complement(8933..9748)
                     /locus_tag="VGA92_03380"
     CDS             complement(8933..9748)
                     /locus_tag="VGA92_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9845489.1"
                     /translation="MQDRDELITMLKHFDLEEIDAKMYIGLLQTGPLSVGNLATKLDI
                     ERGKAYRSLEKLRSIGLITTTLSNPIICKPVEPQEALTNIIQRKENEFVTMKKLADRL
                     SGDLKDITRKQQPIETASVSVIQGRHNIYSRVGKLLQDSGSITYIVTTGKDLLRMYHT
                     SIPEKIKICKNNGGQIRIVTDVGDETTIAIINKLGASEIRLGKLPSKSRMIVENGKHL
                     IMSGGFSETMDMNDENDSVLDSNSIEMVDNMYSLCEHLWRCSKAVEPLNRIAK"
     gene            complement(9830..10975)
                     /locus_tag="VGA92_03385"
     CDS             complement(9830..10975)
                     /locus_tag="VGA92_03385"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX9845490.1"
                     /translation="MAQVLGLIGGGQLGMMLTEAAKKMPQYISEVIVLDPTKNCSAAQ
                     VGAKQIVADYKDKDAILELASKVDILTYEIESGNSEVLKMAEAKTTINPSPETLRIIQ
                     DKFLQKSFLRENNMPVTDFVAIESLDDLKEKINDFEYPALLKVRRDAYDGRGNYKIKS
                     ENDLEKSYPYFAGKTLMLERFVDFKMEVSVIAARNTKGQIATYPVVENIHENNILRMT
                     ITPARVSPSVAMQAEKVAHDTMQVLHGAGVFGIEMFVTSQDKILINEIAPRVHNSGHH
                     TLQSSATSQFEQHLRAILGLELGNTKLLHPTIMYNILGSDNFQGRYKITAPKLENVYL
                     KMYGKTESKPQRKLGHFNLVDADNTLGAEGLLESLESIKNQIKLEPV"
     gene            11073..11645
                     /gene="purE"
                     /locus_tag="VGA92_03390"
     CDS             11073..11645
                     /gene="purE"
                     /locus_tag="VGA92_03390"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX9845491.1"
                     /translation="MLTNKPIVGIIMGSSSDSKIMHNAGKILDEFGVPHEDQIISAHR
                     TPTRLEHYARYAEKNGFRIIIAGAGGSAHLPGMVASYTVIPVIGVPIMVYNYKPGKKS
                     KGGNLSAFSGMDSLLSIVEMPNGSPVATVGINKAANAGLYAVKILSNEFPQLKSKLKK
                     FKEKQHKLVVEESDLMRKTGLVKFVQKKFK"
     gene            complement(11648..12856)
                     /gene="ilvA"
                     /locus_tag="VGA92_03395"
     CDS             complement(11648..12856)
                     /gene="ilvA"
                     /locus_tag="VGA92_03395"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX9845492.1"
                     /translation="MIPTYSDIVKIRNSYSDIIRKTPLYYTETFSRMSGSKVYLKAEF
                     QQKTGSFKLRGAYAKIKSLSPEEKKHGVIAASAGNHAQGVAYASKLEKIPCTIIMPVT
                     ASPAKVAATRKYGAKVLLDGINYDESWAKAQQISKKTGAKIIHAFDDPQIIAANGAIG
                     LEILEDLPDVDEIYVPIGGGGLAAGILVAVKEKNPKIKVIGVESKAFPAMKNSLKAGR
                     LQSVKGNRTIADGIAVKMPGKMTFKIINELIDDIVLVDDVQIVKTMFLLMERAKMVVE
                     PAGAVTLSYLLSARPAPNKKVVPILGGGNVDMYLLGQIVAKGLAAMSRMVKIFILLKD
                     KPGALKEVVDEIASLSVNIVEVVHDRLSSHIDAGTAGVTLSLETEGREHAQRLIAHLK
                     AKNIQFKLLS"
     gene            complement(13114..13950)
                     /locus_tag="VGA92_03400"
     CDS             complement(13114..13950)
                     /locus_tag="VGA92_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845493.1"
                     /translation="MRLGTKTADVFFNNLGQKTGRISQLFQKNIEALTKLLPVKVMLG
                     DTTITDQTTFDPQSIHGFYGKIINKLSEWRSDGISTTNDEDLRRIFVKFERSIGSYVL
                     LWHISLQYHALLYYKPDIIVQKIQKELAEIMDHTIDKEKELAELTDELIKERLASLGY
                     KEIDEQKLFEVLFDNDKIREEILSEVNKKTDYDFKAKDQRKKELFKELDNLLVETYQT
                     TSVLLDENKLIAGEEGCLCTFDLDFVKKKSREAIFDPKRMSSQTKEDIEKAIDEIIRI
                     LS"
BASE COUNT         3757 a         2984 c         2723 g         4531 t
ORIGIN      
        1 tgacaaggat tttgttgact ttgaccctgt ctccgaactt ccactgtact ggcgggtgtt
       61 catcagatac ttccaaaatc ttgatcttca tttccttgtt gccaaagacg ttgtggctta
      121 caagcattcg ctcgtctaca aactcctcaa agctcatgat tctagtatgt cgtttagcta
      181 atttaaagaa ttattcaaaa ttgtcggtgt tggcattctg attgaatgtt ttggcgtttc
      241 tgctcacaaa aggcatttgg taatcatcca aagtcaattt ttggcgcttt ccctcaatta
      301 tggaatatgt gctgccgtcg taattacatg caatgtctga aatcatattt tctttgtccc
      361 agactttgta gtattccctt agctcccgtt tttcatatag gctaagccca attcgttttt
      421 ttgagaggaa tttgaaggca aaaaccacct ttcttgtatc gtatagctca aaggtgtctg
      481 tccatcttag gcatcgcaat atttgatcaa agggaaccac gagtgagttg gtggttccag
      541 atttggcctc aattgtaaat atggtgcagt tttttgtgct cacgacaaga atgtctggca
      601 ggccgacact tggggatccg agtctaaagg ccttccatcc ttctagtgca ttgaatcgct
      661 ttactaacgt gtcctcccaa tgataaccgc ggcttcgcct tgtccttgcg attcttacat
      721 tgtctttttt tgatcgaatc ttgacttgct ttttactggc taaaacttgt tcgagctgga
      781 gctttttcaa cacaatgatt ggtagaattt caagttaaag tggatgtggt tataatataa
      841 taaccaactt gatttaaaga tctgactttt cgggcccaaa cgtaaatttt ggagaatcat
      901 caaaactaca actgatgact cggatctgtg ctccgggttt tggttctgtg ttgcctgaaa
      961 tagtacccat tactcgtaca gtgttttcaa attccacaat acaaaaggtc ctgccgtcct
     1021 ttgatgagca ttcaacaata attcctggct ctttgatgcc tcgccaagaa aggctgccaa
     1081 aacaatgact gcaaaaatcg tttgggggcc atgttacctt ttggcatttt gaacactcgc
     1141 ctacaacaaa ccttcccttt tttagctcag actcaaagac actcatgatc tcatgaccaa
     1201 taccgtagat gatgtcgcag ctgcagacat gttttgaaca agaccgattt ttgcaccatc
     1261 gacttgcctt tttttggcat tgccttctag ctggcatgca atttcagcta cttgggcaac
     1321 ccctgtggct ccaagtggat gacctgagcc aatcaggcca cctcgcgggt tgattttttt
     1381 actgttcgta ttgtaaaggt ctgcaacata ttttccagcc tgtccactcc gcactatttc
     1441 taggtcttct aaaatcatca actcgcatat ggaaaatgcg tcatggattt cagctacgtc
     1501 gatttggctt ggctcaattt ttgacattgt atatgcctga gcgaccgcat cggcagtgga
     1561 ctgtagcgtt actaggtcgt tttttgtaaa acttgcagag gtggttttct ggccaatccc
     1621 tgtaatccag actggcgagt cggtaaattt tctgactaca tcttcggatg ccaacaaaac
     1681 agcagagctt ccagtgcatg gccttgagca gtcaagcaat ctgagatcgt cagtcagcct
     1741 ctttgagtgc attatctcat ccatggagtg gtgttttggg taggctgctg gattgtcttg
     1801 ggcgtttttg tggttttttg cagaaacgta cgccagctgc tcgtctgttg ttccaaactt
     1861 gcgcttgtgt gccctcgtaa acattgatgc ccaaaaaatt gggtgcttaa actcgccacg
     1921 cgagacatcc caatcaagca cctgtccagg actgtcaaac cgttctgcac ctacaaccag
     1981 tgctacatct gccaatccgg cctgaatgta ggaatatgcc gaaattaccg agtttgtccc
     2041 agaattacag aggctttcca cactatggga gattttcggt gttattcccg acatttcggc
     2101 aacaattgat gagaggtatt ttttgttttc atttgttgaa actaaaactg cgtcaacgtc
     2161 tttttgatct aggttttttg tttgctcgaa cactagtttt gttgcagaaa aaagcaggct
     2221 ctctattgga gtgtcatcga cagaaaattt tgtcgtacaa taactggcaa ctgcgatttt
     2281 cttcaaggta tgtcactgaa tgtttttgtt agtaaatcaa atgacgcttg tacgtcacca
     2341 attgggttga actgaattat cggatgagta atgccggcat cataggtacg ccttaactgt
     2401 tctttgcact gttctggcgt tccgcaaatc gcaagtgact ctaacatcgc atctgtgaca
     2461 gactcgtggt ttgattttag tccggatttt aggtattgtg agtaaatact gtcggtttct
     2521 tttttaaagc cattttctgc aagaaacctt ctgtaaatat ctccaacgga aatgtaaaat
     2581 gccaaagttt tgcgcgcccg atcttttgcc agctgctcgt cttctgagac gcaggtgatg
     2641 aactgacagg ccacatcaat tgttcgcttt gactgcattt ttgcgatggt gtttttcatc
     2701 tcggaaattg gccttaggta aaaaatcacg ccatcggcaa tcttccaagt tagctctatc
     2761 attttttggt ttattgctgc aatgtaaatt gggatgttct gtcttggtgg tttgataaga
     2821 agtgtaaatc cgtttaggga aaatatctca ccggaataat caattttttt tccagatagt
     2881 gtcagtcgga ttatttccac gtattcctgc atgcgtttga gtggccttga gaatttgtag
     2941 ccatgcagcc cctcaacaat tggaatactg ctggttccaa gccctaagac gagtctttgg
     3001 tttgataatg tgtctattgt agcggcgccc attgcaatta gtgttgggct acgcgaaaat
     3061 atgttgataa tagaagagcc aatctttgaa ggcgtttttt ggcatactgc acttagcatt
     3121 gcaaagtttt ccatgcccca cgtttctggc acccagaccg tatctggtct gttttttgag
     3181 atcttctccg cacatattag tagatcagaa atagaaagaa gtgaaccaag actatatgat
     3241 aatttcaatg gccataccta actggagacg aatttttcat attttttatt cttcattata
     3301 gttgcacatt cttccaagtc tttatgtgaa tcaatggaaa accaaaacgt atctttgaac
     3361 tttattccag ataatttttt tgccttggcg tatttgtgaa atgttatttc ctcaattgag
     3421 ccatttgatg gtatgtcctc tagtgttttt ttctcaagat ggtaaatgcc tgcattcatc
     3481 caaacatttg agatagattt tttctctcga aaatcaacta tcacatctcc agacaagtcg
     3541 actgtcccaa actttgttct aagctcaacc agcgctattg aatttggctt tttgaaaagc
     3601 ttgcgcacat cgattgttgt tatgacgtct ccgtttagaa ccaaaaacga ttttcccctg
     3661 atgagtttgg cggcattctt tattgcacca ccagttccaa ggggcttttt ctctgtagag
     3721 tattttattt tgcagccaaa attttgtttg tgtgcaagat agttttcaag ctggtctgtc
     3781 ttgtagcccg agcaaatcac aaaatctctt actccaaatt ttttgagata cctgatttgc
     3841 cactctataa ttggtatgtt gtttaatggc actagggact ttgggacata atctgttatt
     3901 ggcttgagtc tggtcccttt tccgcctgcc agtattactg cttgcacaca catctgagaa
     3961 aaattcgctt ttaaaaacgc tgattttctc tagtcttact catgtttttg cctgagtgac
     4021 ccaacgccaa gaactgaaag aatcactatt ccgaagtata atgagagcga ccaaatcaac
     4081 cagtacgggt cagactggat tgcctcaaag ggcaaataca gtatttgagt tccaatccca
     4141 ccaccatcat atacttctat attctgcaag tgttggaaat gaaacggctg attcataaaa
     4201 accagggcaa gcgagtaagg cgtataccaa aaaatattga tcagtccaaa cgctaatgca
     4261 agtattccga ttattttgta tcttgttttc attttctaat ctcattccta tgttttccat
     4321 gtctatacgt taacggcaac aaaaccaagc tgatcaaaaa taatacattt aattctgtaa
     4381 tggctgcatt tcttgttaat gcacgtggca ttcttgaaat gtcgtccagt atgataaatg
     4441 aataaaagta aacccaacca attcctaaaa atattgcaaa tcctaggttg atataaaaaa
     4501 gaatttttga agttttcatt ttcgccttct aattataaac acggcagcac cagctatcag
     4561 acctgcagat actccaatca aaacaaaaaa cagccacccc tgagtaaaaa actgtagata
     4621 gtacctggga tcatcctcaa agaaaggacg gtcatattct agtggaaatg atatttctcc
     4681 tctggactgg taataagaaa acacgttgtg gttttggctg ctgccgtcca tccattctgc
     4741 caacattcct tcatctttgg catcaaacat ttctgccttt ttttgctcca tccagtctga
     4801 gcctttgtag tcatagtatg gagcccaagc cgctctgtat tcctctctgt ttactggcag
     4861 cacatcaaag catggggcgt cagaccagtc ttcatttact gcacatgatg caaaagcaaa
     4921 attagaaaat atggcaaaca ttccaaaaag cgcgaaaatt ccaagcaaaa ctacaatctt
     4981 gctcatgaag acagcttttt cgtatattcc ttaaatcttg ctcagcttac cttatgaaac
     5041 tgttctaggt cgcatttaga agaaaaaaat gcaaaatctc tttttgtaga aattatttag
     5101 tttatgattt tgtccaatgg ggatgtcgag aagcctgata catcagatct tcggagccta
     5161 atggggcacg aaaaatctgt cagcctttcc tattttgccg gtacccatcc gccacaagaa
     5221 aggcaaatgt tcacaccgtc ttcattttcg tagttttcag atcctggcgg acactcgcat
     5281 cgtggaattt gatcttccat attaacaaaa cgtcattcaa tctattaaga tttgatgcgg
     5341 atcggtgagt aagagaaaat cggcatccaa ggcttaaggc caaaaacgat agtatcttgg
     5401 cgacagccct aaacgcctag gtatcaatgt actttgaaat taatatctgg tatggtgttt
     5461 gattcggaca catcaagtag aaagaaatac actatttctt ccaaacgatc tttccgtttt
     5521 tacaaaggca gtgttttttg gtttttcctc agactctatt ttaaaaactt gtaatttttg
     5581 tatggtgtct ttttccttag gctctgtgga aacttgcaac tcttgcgagc tagttttttc
     5641 ctcatagtcc atgttgacta gttcgccttg cgagtatcct gatttggcat actgcccaag
     5701 ctcataaaac ccatcgccta ctgctttttc tatgtcagaa tcactaacaa atgacaacgc
     5761 agcggatacc caccagcttg tttttaatgt cagtgtggcc ttaacgatgg ttgccccatc
     5821 aggcgttccg tcgaatccaa ctcttttttc aaggttggtt atgatctgcg aaccctttag
     5881 gtcacctgag attatgtcta ttgcaaacga accatcttgc agtattgatg atcttacgtc
     5941 agcatcatga gttccctgcg caataattat gaattttgcc tgggttgtct ctgagcttat
     6001 ctggacgtct tttacaagcg taggaaaaat ttccttgtag ctccgaaggt ctgtaagaac
     6061 aatagcaagg acttcttttc tgacatcgat ttcttttgtt acttctacag ccctttctgc
     6121 aaaaactgga gtggctgtga tactcactaa cgacgccaat aagactaacc caatagtgtg
     6181 aagatttttt attgcaatat atggacgaag atctgatcaa taataactac ttggtaacta
     6241 agttacctaa tgattgctct atacgtgtag tttttttcag aaaagatcaa ttgttactag
     6301 tccaaaataa tagccaacca atgtcagtgc aactccatac acacaggagc cgccaaacgt
     6361 gtaaagtaag aatttgatgg gtttcattcc taagagccct gctggaattg agatcatctc
     6421 gcgcaagaca ggtactagcc ttcccaaaaa aactgcctta tctccgtgtt tttcaaacca
     6481 tttttcaatt ttggcaagac gttcttctga aatcttggcg tatttgaggt acctgaggag
     6541 tgcgactcgt ccaagcttta tgcagattac ataaattatt gtagacccaa ttgttgctcc
     6601 caacccacct gccattccaa gaccgattgc ttctggcaat gacatgccgt tttgtgatgc
     6661 gatatatccg gcaaatggga atactgccat tgttggaatt ggaggaaaaa ccgtctcaat
     6721 tacggctgca gcaaaaactc cggcatacag ataatcggat attagtgagc taatccattg
     6781 gatgaagggc tggatttcta gcaatttttc tgctctgtta tgtaataatg cagttcagta
     6841 taacactcga cgcaaaattc ctgatcattt gtgtgaatgc agtcatacgc ttcatcggtt
     6901 tttgcgttgc atttggcgca gacagtcatt ttgattttcg aatttttatg gcacctagtg
     6961 ctatttgaaa tgcgcctact ccaaacaaag gtgctgcctc tgccattggg tttctttcca
     7021 gtgtttcagc tggagcgttt gctaaaatgt aagcacctcc tacaattatc atgatgccaa
     7081 ctacaaacaa catggcagca agtggtcttg atggctcatt ccgtgatatg aaaaacccga
     7141 tgattggtag cgccatggct ggtccgccaa gcaccatccc tctcgtcttg tggtcaaatg
     7201 gcaaaaatcc ataatctgcg ccagctgcta cccctacatc cgcataatat atggcaagca
     7261 atcctatagc aatgcccgta attatcagcg ctgcttttag tgccattact tcggcctctg
     7321 cttaattaac taataaatcg tgctggttgc aataattcca caccatccac ggtttcttag
     7381 tctattcaaa acaacttttc tgaatttggt tatacattta aaaatgaatt tgatatatag
     7441 tgggtgataa aagtgtcaac tgacgtatta cacgaaatcg caattgacga agatgacaaa
     7501 aaacaaaaag cactcgaagt cttttctgat aattatacaa ggataattct tgcaacaatg
     7561 atggaaaagc caagatccgc attacagatt gcggaagaaa ccaaaatacc actcacaact
     7621 gtgtatcgta agctgcacca tcttttggaa aataatatga ttcgcatctc gggtcagatt
     7681 gcagaaaatg ggaaaaagaa ttttctttat aaaagcaaga taaagtcatt tcatatcacc
     7741 tttacaaagg acaagcttgc aatcctagtc aatactagtg gcacatgtaa attctgcctg
     7801 caggcccact agatttggac tagtttgttt tgtgttttaa ctcaagccgc taaacaggct
     7861 aaaaataata tcgccataaa ttagctgaat tacaaaacca gcaaaaatga atatcatgta
     7921 tgggactccc ggtgtaatcc acgtgtcttt tttttcgcaa aatgctgcat actctgcgtg
     7981 ctgaaatgac aaattgagct ttttgcgttt gccgtttttt cgttcaattg agaatccaaa
     8041 ctttggatta gcggccctgt atccgatgaa catggcaaga agctttctcc cctttgtttc
     8101 atcaaatcct tcgaaaatat cgatttttct agcaatcaga attacatttc gtactgcatt
     8161 gatgaacatt ggaactactg acaaaagaac tgcatttgtg aggacggtaa acggtgtaat
     8221 cgtgccttct actagtgtca tgtttggcgc aagaactgct aaggcaatca atgcaaaggc
     8281 atcggcgcct ccaaaaaggc caaatcgcca tattatgatg gcaatgggcg caacaatcat
     8341 cgatattcct atttttaata attcatcatt taggtcaccg gcaaatggga tgagaatggc
     8401 acctattgca ccaaacacca cccagattat gtcgtttatt tcccgttttt tgatatctga
     8461 tgctgttgca atacctagca tggatactgc caaaataatt ctaatttgat ctagtgcagc
     8521 taattctaac atggtctatc cgttattact agatcaaatt tataaaaatg agctgatgcc
     8581 caatgaattt tactagggta ttgtattata acccgcttgt taaataagat gccaccatag
     8641 attttgctct gttagagcta ggcaaaatgg aagaacgtcg tggtattgat atgaactcct
     8701 acctgttacc atgtaaagcc gaaacaggat tgagttcaga cagattaccg caggaactgc
     8761 gggaatgcac gcctgtagag atcagaagct cggtcgttga agcaggaaga tgcagttgtg
     8821 gcgacatgat tgcactccca cataattggg ggattatcag ggtcatctga taatcagttc
     8881 acttagtgaa gcttgcgttc tccattttca aatttctagt tctatcaata cgctattttg
     8941 ctatcctgtt taacggctcg acggcctttg aacacctcca taaatgttcg caaaggctgt
     9001 acatgttgtc gaccatttca atagagttgc tatccaaaac ggaatcattt tcgtcattca
     9061 tgtccatcgt ttcgctaaaa ccgccagaca tgatgaggtg ctttccattc tccactatca
     9121 tcctgctctt tgagggaagc ttgccaagcc ttatttctga tgcgccaagc ttgttgataa
     9181 ttgcgatcgt ggtttcatca ccaacatcgg taactattct gatctgaccg ccgttgtttt
     9241 tgcaaatttt gatcttttct ggaatggatg tgtgatacat cctcaaaaga tcctttcctg
     9301 tagttacaat gtaagttatg gaaccagagt cttggagcag ctttcccact ctggagtaaa
     9361 tattatgcct tccctgaatc acggaaactg aggcagtctc aattggctgc tgttttcttg
     9421 ttatgtcttt aagatcccca gaaagcctgt ctgcaagctt tttcatggtt acaaactcgt
     9481 tttcttttct ttgaattatg tttgtgagtg cttcttgcgg ctcaactggc ttgcagataa
     9541 ttgggttgct cagagttgtg gtgattagac cgatgctcct tagcttttca agcgacctgt
     9601 aggctttgcc cctttctatg tctaattttg ttgcaaggtt tccaactgaa agggggccag
     9661 tttgcaaaag cccaatgtac attttagcgt caatttcttc gaggtcaaag tgctttagca
     9721 ttgtaattag ttcgtccctg tcttgcaagt cgatagagat tattaggaat ctagtatatt
     9781 agtttgtata aaatgtgatt accttgttgt caaatctttg tatctaaatt cagactggct
     9841 ctagcttgat ttggtttttt atcgattcta gcgattcgag caagccttct gccccaagcg
     9901 tgttgtctgc atcaactaaa ttaaaatgac caagctttct ttgtggtttt gattctgtct
     9961 ttccatacat tttcagataa acattttcta atttgggtgc tgtgattttg taccgaccct
    10021 gaaaattatc cgaacccaaa atgttgtaca ttatggttgg atgcaaaagt tttgtgtttc
    10081 caagctccaa gccaagtatt gctcgcagat gctgctcaaa ctgcgatgtt gcactggatt
    10141 gcagggtgtg atgaccagag ttgtgaacgc gcggcgcaat ttcattgatt aatattttgt
    10201 cttggcttgt gacaaacatc tcgattccaa acactccggc accgtgcaac acttgcattg
    10261 tatcatgtgc aactttttcg gcctgcatag caactgaagg tgagacccgt gcaggggtaa
    10321 tagtcatcct aagaatattg ttttcgtgaa tgttttcaac aacgggatat gttgcaatct
    10381 gcccctttgt gtttcttgcg gcaattacag atacttccat cttaaagtca acaaaccgct
    10441 caagcatcag cgtttttcca gcaaaatacg gataggattt ttccaaatcg ttttcagatt
    10501 ttattttgta gttccctcgt ccatcgtatg catctcttcg gacttttagc agtgcaggat
    10561 actcaaagtc atttatcttt tctttgaggt catctagtga ctctattgcc acaaagtctg
    10621 taaccggcat gttgttttct cgaagaaatg atttttgcaa aaacttgtct tggataattc
    10681 gcagcgtctc aggtgaagga ttgatggtag tttttgcttc tgccatcttt aggacttcac
    10741 tgtttcccga ctctatctca tacgtcaaaa tatccacctt tgaggcaagt tctagtattg
    10801 catccttgtc cttatagtca gcaacaatct gttttgcacc aacctgggct gccgaacagt
    10861 ttttggttgg atcaagtacg attacttcgg atatgtactg gggcatcttt tttgcagcct
    10921 cagtcaacat cattccaagc tgccctcccc caattaagcc caaaacctgt gccaacatct
    10981 catcaatcaa aaattggatt aaaaattgtt attcgaaatc tgtcccaaat cagtccagaa
    11041 tcattaaaaa cagcattttc ttttcaagag ttatgctcac aaacaagccc attgtgggaa
    11101 taatcatggg atcaagctca gatagcaaaa taatgcataa tgcaggcaaa atcctagacg
    11161 aatttggagt tccacacgag gatcagatca tttctgccca cagaacccca acacggcttg
    11221 agcattatgc tagatatgca gaaaaaaacg gctttaggat aattattgca ggtgctggcg
    11281 gctctgcaca tttgcccggc atggttgcgt catatactgt aattccggta attggtgttc
    11341 caatcatggt gtacaattac aagccgggaa aaaaatccaa aggcggcaat ctgtctgcat
    11401 ttagcggaat ggactcattg ctctcaatag ttgagatgcc aaatggctct ccagtagcta
    11461 ctgttggaat taacaaggct gcaaatgcgg ggttgtatgc agtaaagatt ctctcaaatg
    11521 aatttccgca actaaaatcc aagctgaaaa aattcaagga aaaacaacac aaactagtag
    11581 ttgaagaatc cgacctgatg agaaagacag gtcttgtaaa atttgttcag aaaaaattca
    11641 aataaactta tgacaagagc ttgaattgta tgttttttgc cttaaggtga gcaatcagtc
    11701 tttgggcatg ctctcgtccc tctgtttcaa ggctcagggt gacacctgca gttccagcat
    11761 ctatgtgtga actgagtctg tcgtgtacca cctcaacaat atttacacta agcgaagcaa
    11821 tttcgtcgac tacctctttt agtgcaccgg gtttgtcttt taacaaaatg aaaatcttga
    11881 ccattctgct cattgcggca agaccctttg ccactatctg gcccaaaaga tacatgtcga
    11941 cgtttccccc accaagaatt ggtacaactt ttttgtttgg tgctggtctt gcgctaagaa
    12001 gatatgacaa agtaactgcg cctgccggct ccactaccat ctttgcgcgc tccattaaca
    12061 agaacattgt cttgactatc tgcacatcat caacaagaac aatgtcgtca attaactcgt
    12121 taattatttt aaatgtcatc tttcccggca tctttactgc tattccgtct gcaattgtac
    12181 ggtttccttt gacagattgc agccttcctg cctttaggga attcttcatt gctggaaatg
    12241 cttttgattc tacaccgatg actttgattt ttgggttttt ttcctttaca gctactagta
    12301 tgccagctgc caagcctcca ccgcctattg gaacatagat ttcatctaca tctggcaggt
    12361 cttctaaaat ctctagacca attgcaccgt ttgccgcaat tatctgtggg tcatcaaacg
    12421 catgaatgat ttttgctccc gtcttttttg aaatctgttg tgcttttgcc caagactcgt
    12481 catagtttat tccatcgagt aggacctttg cgccatactt tcttgttgcc gcaacctttg
    12541 caggagatgc agtaaccggc attatgattg tacagggaat cttttcaagc tttgacgcat
    12601 atgcaacacc ttgtgcgtgg ttgcctgctg atgcagcaat cacgccgtgt tttttttctt
    12661 ctgggctaag tgattttatt ttagcatatg cacctctaag cttaaaggag ccggtttttt
    12721 gctggaactc tgctttcaga tatacttttg agcctgacat cctactgaag gtctcagtgt
    12781 agtatagtgg cgtctttcga attatgtcac tgtaggagtt tcttatcttt acaatgtcac
    12841 tgtatgtagg aatcattaga attgatcgca ccaatctcac tatatttaat ttcttgtctg
    12901 attttgcaac aaaaccaaaa atctagcctc aaaatcaaat gtgtgtagat tagctttacg
    12961 ctcgattttc tgcatactgc ttaaatacat tgacgaggat cgtacaaagc tggtcaaagg
    13021 atcgttctcc ccttgagcta gatcaactag ttcatttgat cctttgacta ttgattatta
    13081 caaagtgaca atagcattgg atataagaat ttcctaagat aaaatccgaa ttatctcgtc
    13141 aattgctttt tcaatgtctt ctttggtttg actggacatt ctttttggat caaaaattgc
    13201 ttctcttgat ttctttttta caaaatcaag atcaaaggta caaagacacc cctcttcgcc
    13261 tgcaatcagc ttgttttcat ctaagaggac tgacgttgtc tggtaggtct caaccaaaag
    13321 gttatcgagc tctttgaaga gttccttttt tctttggtct tttgctttaa aatcatagtc
    13381 tgtctttttg ttcacttcgg agagaatctc ttcgcgaatc ttgtcgttat caaacaatac
    13441 ctcgaaaagc ttttgctcgt caatctcttt gtagcctagg gatgctagtc gttcctttat
    13501 gagctcgtct gtgagttccg caagctcttt ttccttatca attgtatgat ccattatttc
    13561 tgccagctct ttttggatct tttggactat gatgtctggc ttgtagtaaa gtaaggcgtg
    13621 gtactgcaga gaaatgtgcc ataaaagaac atagcttcct atactgcgct caaactttac
    13681 aaaaattcgc cgtaggtcct cgtcgtttgt agtcgatata ccgtctgatc tccactctga
    13741 gagtttgttt attattttgc catagaaccc atgtatactt tgtgggtcaa acgtggtctg
    13801 atcagtaatc gtggtgtctc caagcattac tttgactgga agcagctttg ttagcgcctc
    13861 gatatttttt tgaaataact ggctaattct tcctgttttt tggcctaaat tgttgaaaaa
    13921 cacatcggca gtcttggttc ccagtcgcac aaataggcaa ggttttgcag cctaaaatgc
    13981 ctaatgcttg ccaaa
//
LOCUS       DASWKH010000045         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_94167, whole genome shotgun sequence.
ACCESSION   DASWKH010000045 DASWKH010000000
VERSION     DASWKH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94167"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VGA92_03405"
     CDS             complement(<1..173)
                     /locus_tag="VGA92_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX9845494.1"
                     /translation="MKQNFLDALNERILLFDGAMGTEIQKFNPKPEDFPDGKDGFNDG
                     LILTRPEWIKKIHR"
     gene            complement(191..961)
                     /locus_tag="VGA92_03410"
     CDS             complement(191..961)
                     /locus_tag="VGA92_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methenyltetrahydrofolate synthetase"
                     /protein_id="HEX9845495.1"
                     /translation="MTVRYEINPPRVIQNKVLSHKEVQGSLAKVKKRITQISKICDGI
                     HLTDSVLGIPRISPITTGAIIRNDGLKLDITASLRVRDRNITALTQSIYDAVLLGLDG
                     LLVLKGDEPPSGPKDSKLIPSDVVKYFKGLGFGKNLDFFLSISANPNFDKIQKKIDAE
                     PTGFVTQVIQSPEQVFRIVDQLKPQGFKVIPCLMIPSQNNSKSAKMLGIDFARYEENV
                     LEFISEIYKSTKDILITSPNDFDSARKVLAGLKGLKRK"
     gene            1038..1601
                     /locus_tag="VGA92_03415"
     CDS             1038..1601
                     /locus_tag="VGA92_03415"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9845496.1"
                     /translation="MSLVHIRKLLSSNISPYIDDQNTKHAAVLVVIYGDEPKIIMTQK
                     PNTLQQHAGEISFPGGKFVQDDEDLLNTAIRETKEEISLEVMRNQVIGQLRPVHTRNS
                     DFTIIPFVAILQDISDLKPNSEVAEILHIPLLPLLSGLKIDDDANHRALFEAYVATYQ
                     NKIIWGASARILKQMADIFRKNNLLQS"
     gene            1657..1815
                     /locus_tag="VGA92_03420"
     CDS             1657..1815
                     /locus_tag="VGA92_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HEX9845497.1"
                     /translation="MAARKHSGRKIRLLKKKKQNSPVPAWIILKTKRQVRSNPKRRQW
                     RQTDVEVG"
     gene            1817..>1964
                     /locus_tag="VGA92_03425"
     CDS             1817..>1964
                     /locus_tag="VGA92_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9845498.1"
                     /translation="MSQEVERVYTINLGKVWLSPNNQRAKRAINMIREFATHHMKTEQ
                     IKIDP"
BASE COUNT          589 a          408 c          393 g          574 t
ORIGIN      
        1 cgatgaattt tttttatcca ctctgggcgt gtcaggatta acccgtcatt gaatccgtct
       61 tttccgtcgg gaaagtcctc aggctttggg ttgaattttt gaatctcagt tcccatcgct
      121 ccatcaaata acaaaatcct ctcatttagt gcatccagaa agttttgttt catgtataca
      181 actatagaat ttattttctt tttaatcctt taagcccggc tagaactttt cttgcagaat
      241 caaaatcgtt tggcgacgtg atcagaatgt cctttgttga tttgtaaatc tctgagatga
      301 actccagtac gttttcttcg tatctggcaa aatcaattcc tagcattttg gcggacttgg
      361 aattattctg cgaggggatc atcaggcatg gaatgacctt gaacccttgt ggctttagct
      421 ggtctacgat tcgaaaaacc tgttcgggtg actggattac ctgcgtgaca aacccagttg
      481 gctcagcatc aatctttttt tggatcttat caaaatttgg gtttgcagaa attgagagga
      541 aaaagtccaa gtttttacca aaacccaacc ccttgaaata ttttacaacg tcacttggaa
      601 taagcttgga atcttttgga ccacttggcg gctcatcgcc ctttagtaca agtagcccat
      661 caaggcccaa aagcactgca tcgtatatag actgagtcag cgcagtaatg tttctgtctc
      721 gcaccctaag gcttgcagta atgtctaact ttaatccgtc atttctaatt attgcaccag
      781 tggtaattgg cgaaattctt gggattccca gaaccgagtc ggtaagatgg attccatcac
      841 aaatcttgct gatctgggtt attctttttt tcactttagc caacgagcct tgaacttcct
      901 tgtgagagag gactttgttt tgaatcactc gcggggggtt gatctcatat cttacggtca
      961 tttgtttgat ttctaattta cttgattata acttttggag cgataaacca ataatggcga
     1021 ccatgttttc atatttgttg agcttagtcc atataagaaa attactctca tcgaacattt
     1081 ctccgtacat tgatgaccaa aacacaaaac atgccgccgt cctagttgta atctatggtg
     1141 atgagcccaa aataataatg actcaaaaac caaatacgct gcagcaacac gcaggtgaga
     1201 tttcatttcc tggaggcaag tttgtacaag acgatgagga tttgctaaac actgcaatac
     1261 gtgaaacaaa agaagagatt tcacttgagg tcatgcggaa ccaagtgatt gggcagctaa
     1321 ggccagtgca tactcgcaat tctgatttta caatcattcc atttgtggca atcttacaag
     1381 acatctcaga tttgaaacca aattccgaag ttgcagaaat actacatatt ccattgctgc
     1441 cacttttgag tggacttaaa attgatgatg atgccaacca tagggcgctc tttgaagcat
     1501 atgttgcaac ataccagaac aagataatct ggggcgcatc agctagaatt ctaaaacaaa
     1561 tggcagacat cttcagaaaa aacaatctct tacaatcata attttttcaa aacatccgag
     1621 attcatttaa aaagagggta tatcgcctgc ttttttatgg cggcacgaaa gcactctgga
     1681 cgaaaaatac gactgttaaa aaagaaaaag caaaactctc cggtcccagc atggattatt
     1741 ctaaagacca aacgacaggt gcgctctaat ccaaagcgaa ggcagtggag acagactgat
     1801 gtggaggtag gctaagttgt cgcaagaagt agaacgagtt tatacaatta accttggcaa
     1861 ggtctggctt tcgccaaaca accagagggc aaagcgtgca atcaacatga ttagagagtt
     1921 tgccacacac cacatgaaaa cagagcagat caaaattgat ccgg
//
LOCUS       DASWKH010000046         9551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1582062, whole genome shotgun sequence.
ACCESSION   DASWKH010000046 DASWKH010000000
VERSION     DASWKH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582062"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VGA92_03430"
     CDS             <1..148
                     /locus_tag="VGA92_03430"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9845499.1"
                     /translation="HVRYVHSDGRKTVVPVHGNEMLGRGLINEILKQVKLSREEYEKL
                     RLKI"
     gene            complement(228..422)
                     /locus_tag="VGA92_03435"
     CDS             complement(228..422)
                     /locus_tag="VGA92_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845500.1"
                     /translation="MSVKIKDIKTKVIKEDEGYSIACSALGVYSTGKNLQDAKKNYLK
                     ALELHLSVLREKATEPFNLH"
     gene            complement(499..735)
                     /locus_tag="VGA92_03440"
     CDS             complement(499..735)
                     /locus_tag="VGA92_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845501.1"
                     /translation="MKQTSITVSKKSKDQWSKLKNHPNESFENMINRVLKSQAEEDAE
                     LLTDANILEIEQSIKDIKKGKFKTLKQMKAKYGL"
     gene            1010..1222
                     /locus_tag="VGA92_03445"
     CDS             1010..1222
                     /locus_tag="VGA92_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845502.1"
                     /translation="MTEGRKFTIRTTKDPTGGYSGRCLELPAAISEGETLEELMVNMK
                     EVIQIVLESIEKDCPADEKIVVTIPT"
     gene            1219..1434
                     /locus_tag="VGA92_03450"
     CDS             1219..1434
                     /locus_tag="VGA92_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9845503.1"
                     /translation="MSLRNHNWREVLKVLTEFGYVLDRQRGSHMILKNSSGNIVPLPR
                     HDPIKPTTLKSILDQAGISLEEFLDKI"
     gene            complement(1465..1688)
                     /locus_tag="VGA92_03455"
                     /pseudo
     CDS             complement(1465..1688)
                     /locus_tag="VGA92_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078502.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(1681..1890)
                     /locus_tag="VGA92_03460"
     CDS             complement(1681..1890)
                     /locus_tag="VGA92_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845504.1"
                     /translation="MQYRIIIQQDEDRIYIAKCQSLPGCISQGKTKAEVLDNIKDAIS
                     GYLASLKKHNEPIPSIEEELVEVSA"
     gene            complement(1965..2099)
                     /locus_tag="VGA92_03465"
     CDS             complement(1965..2099)
                     /locus_tag="VGA92_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845505.1"
                     /translation="MQRQDKDQKVSQEIEKIMEDIDTGKVKTSRFTADEFLKFVDKTE
                     "
     gene            2502..2957
                     /locus_tag="VGA92_03470"
     CDS             2502..2957
                     /locus_tag="VGA92_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845506.1"
                     /translation="MGKHGRPSKAEQLHIEKTLRSYFEKMLSPSFAARDAGININTVK
                     KYYRVFSDEIRSSEHPDFIQRSKNIIQNCSLAFDVQLSKLYTLQDKLEAQINQEIKQK
                     GKIPPALYKISIRLSTEIAGLLFKKANLTLSPTADITLSRYIKESGIVA"
     gene            2941..4725
                     /locus_tag="VGA92_03475"
     CDS             2941..4725
                     /locus_tag="VGA92_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845507.1"
                     /translation="MELLHSSWVLKHDKLARNSFTRIKKRHSGHALITPPSNPLEWIE
                     KVRPIVEGKTRTFLASPFWKEIYLDNYHSKIIVGGRQIFKSTYITDILACEATSAPGI
                     QVGYVTFDQSSLNGFSKQKLQIGTFSQNPVLSRFPRNKLGNIGEISLRNGSTIYCMTD
                     NNEYIHVEGKSLNHCILDEAQYQDIVHIEKIRQTMMATKGKLSILGIGGESGSPYEKL
                     WQNSDQREWVYDDPNWRDRLQFDGDGLIADKYLVDVLSGRWVPQKPENTMYHGYHIPQ
                     TIFATNPLTIDDAINKYRISPFYSIEYQKKNNPKSFFSSHVMGEFYRSERRPITPQMV
                     HSCMNHYRYIGLMTFSEIAVLKDVMQDSINVSMGVDFGSGNPSNTVISILIHWKKSDR
                     IQLAYIDKRPAEHQLDQAEHINRLYRASRCDIGVGDLGYGAIQVKVIQDGGSNRTTGK
                     KFPGVDNENFYGCRNISNESKPMQVHPNVIDEHGEEIERISIDITTAIQKFIDLIGTS
                     KSHPRRADESSRRSRLLIPFHVDREYETDWLVSDFTSVTRKDLGRKDLDPRQNAKKEF
                     NHPKDSVMSIIYALVGLEQDTEFHWISA"
     gene            4732..4962
                     /locus_tag="VGA92_03480"
     CDS             4732..4962
                     /locus_tag="VGA92_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845508.1"
                     /translation="MQAKGKKHNIHGQDELLRNLQTEKIDDASGLLRKLKQVTGMKLQ
                     VFITDGLGSYAEAYQKEFCKGETRTLDTSLAR"
     gene            5884..6219
                     /locus_tag="VGA92_03485"
     CDS             5884..6219
                     /locus_tag="VGA92_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845509.1"
                     /translation="METLADDKKNSKPYLGIVGYLDFLLEATKKVNPTLRNVATVAKI
                     ISEHKYFETKSQLNKKLPKAMQYPTFNFILDYLQKTNMIQLNPDGSIVWIYPTSQKLK
                     KRIIQAKPL"
     gene            6475..7290
                     /locus_tag="VGA92_03490"
     CDS             6475..7290
                     /locus_tag="VGA92_03490"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX9845510.1"
                     /translation="MVTKLIRLGSTELKLILTLEEEGKKIFNTADAYRILKSSKDSVN
                     AALYRLRKKGRIEEIERGKYLLIPAKAGYSGKWAEVPFIIASEIVDPYYIGFASALNY
                     WGMTEQVPSTTFVVTTKRKRNLEYGSLKFKFVTFSKKRFFGIVEEEIAGEKFKISSRE
                     KTIVDGLNYPRYCGGLDEIVKGIWESQDEIDFAKIIDYAKEMRNDSVKRRLFYILDIL
                     ELKKKVSVKDLNKIPKGLKWLDPSGPKNAIEYSKEYGLIINRTKKDLMSWRGY"
     gene            7291..7509
                     /locus_tag="VGA92_03495"
     CDS             7291..7509
                     /locus_tag="VGA92_03495"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9845511.1"
                     /translation="MLSKDDIMKIAGEKKINPSAVSKDYALGWLLFGISKSSIGNKLI
                     FKGGTALSKVHFPEGCGLSHHVFVKFKL"
     gene            complement(7499..7864)
                     /locus_tag="VGA92_03500"
     CDS             complement(7499..7864)
                     /locus_tag="VGA92_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845512.1"
                     /translation="MRIASIGVVCPNTCDPETEVRPIIEQFFHSYKPSYRIESPRNSP
                     YQIVIWDELTIDENSNLQDMAHKIRADIHDSYVFTVNYNHKKTKWEKWNPVLGIGLTI
                     AGLILTGIGLYLIFYPPIT"
     gene            complement(7899..8129)
                     /locus_tag="VGA92_03505"
     CDS             complement(7899..8129)
                     /locus_tag="VGA92_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845513.1"
                     /translation="MPEVEFRVLCANTEIACNRVKTLMNDFSSIKDAKKDHVYDHFGT
                     RKFDSVADAIKRKNQLYTQAGNDVCDIKLIIK"
     gene            8428..8646
                     /locus_tag="VGA92_03510"
     CDS             8428..8646
                     /locus_tag="VGA92_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845514.1"
                     /translation="MTQVGQSISFSEELITAIRVLASSKKQPLSQFLEFQLREVSIIK
                     ETIRKLEKIPDLPPMVKGSIPYSTVEEI"
     gene            complement(8643..9101)
                     /locus_tag="VGA92_03515"
     CDS             complement(8643..9101)
                     /locus_tag="VGA92_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845515.1"
                     /translation="MQQRKNLDLESPFREICRQDAEQIFTNEIQPQNNIDLSYLVGKN
                     ISSRYVLVYDYQGKILGFLTFIDKGTHFHLDLVENNRKHKETAIHKPGYQLIKKLEDL
                     SLTFGYPKITLYSTDSRLSYYQDVHEYTVTGARRSDPHYDFLTPMEKILT"
     gene            complement(9153..9389)
                     /locus_tag="VGA92_03520"
     CDS             complement(9153..9389)
                     /locus_tag="VGA92_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845516.1"
                     /translation="MTQIIPPEEFTGKRMLPVQLLHQRSKSETTSSVIKRKFGSEIKS
                     YHDIMKEKEPLYRALAYNCHRMTIISCLSSMISR"
BASE COUNT         3139 a         1657 c         1804 g         2951 t
ORIGIN      
        1 tcatgttaga tatgttcatt cagatggtcg taaaacagtt gttccggtgc atggcaatga
       61 aatgttagga agaggtttga taaatgaaat tctcaagcag gtaaaactat caagagagga
      121 atatgaaaaa cttcgtttaa aaatctaatc gatgttttct agactcattt atgcagaaat
      181 ctttatgatt tttttatttt ctgattttgc tttatcctta aaatattcta atgcaagttg
      241 aatggttctg ttgccttttc tcttaataca gacaaatgaa gttcaagtgc ttttagatag
      301 ttttttttgg catcttgtaa atttttacct gtactataaa ctcctaatgc agagcatgca
      361 atagaataac cttcatcttc tttgattact ttagttttga tatcctttat cttaactgac
      421 atatgatatg attatcagct ttaattattc aagtttcctt atctgatgtt ctgccctaaa
      481 cggcgtcatg aattagaatc atagaccata ttttgctttc atttgtttta atgtcttgaa
      541 tttacctttt ttgatatctt tgatggattg ctcaatttct aaaatatttg catcagtaag
      601 tagttcagca tcctcttcag cttgagattt taaaactcga ttaatcatgt tttcaaatga
      661 ttcatttgga tgatttttga gttttgacca ttgatccttt gatttttttg atactgtgat
      721 tgatgtttgc ttcataggtg aacatagatg cctatagtac ttaataattt agtaaaatta
      781 cattgtagga gttagtcctg aaatcatcgc atatctatga taattataat tggactgatt
      841 cttcccatac tttctttagt ttaggatcag aggaaaatat ccatacaact gcgcgggact
      901 tggaagtaat gcttgtggag agatgagttt gatgaaataa gaagagatca cctaacttgt
      961 gggatagttt actacctaat tttatatagc aaacaagcta aatcatgtca tgacagaagg
     1021 acggaaattt accattcgaa ccacaaaaga cccaacaggc ggatattctg gaagatgtct
     1081 tgagcttcct gcagcaataa gtgaaggcga aaccctagag gaattaatgg taaacatgaa
     1141 ggaagtaata caaattgtct tagaatcaat cgaaaaagat tgtccagcag atgagaaaat
     1201 agtagtaact attccaacat gagtttaagg aatcacaact ggcgtgaagt tctaaaagtt
     1261 ttaacagaat ttggttatgt tttagataga cagcgtggaa gtcatatgat tctaaagaat
     1321 tcttcaggaa acattgttcc tcttccaagg cacgatccaa taaaacctac gactttgaaa
     1381 tccatattag atcaagctgg aatttcacta gaagaattcc ttgataaaat ctaatatcaa
     1441 cacattagtt ggtttattgt atcatcagac tagatcaata aatgcatcaa ctgtcaatcc
     1501 tgcgtcttca attatagatc ttaacgtacc tttggctatc tctttatgat taggtactgt
     1561 caatctcaga caaggatttg cttatgtctt aggattatgt gactcccagt ttgatgatca
     1621 gtttcataac ctatctttgc caagaccttg acaaccttga tgctagatac tattggaatc
     1681 ctaggcactg acttctacca attcttcttc aatagatggt attggttcat tgtgtttctt
     1741 taggcttgcc agatatccgc ttattgcatc tttgatatta tctagtacct ctgccttggt
     1801 ttttccttgg gagatgcatc ctggtaaaga ctggcatttt gctatataga ttctgtcttc
     1861 gtcttgctgt attattattc tgtattgcaa taattctctt tgcacttgtt tacttgaata
     1921 ctttacggtt ttgtaatatg aaataagatc taattgccat aaacttattc agtcttgtct
     1981 acaaatttaa gaaactcatc agcagtgaat ctactagtct ttaccttgcc tgtatcgatg
     2041 tcttccatta ttttctcaat ttcttggctt actttttgat ctttatcttg cctctgcata
     2101 ctttgaatag acattcattc tttataaaaa aatactacat tagcgaatta tttgaatacc
     2161 ttgacagttt aaaatctgaa caaaaatcat gttttgagaa aatactcttt tgagggtgtt
     2221 ttttgctcac cacatatttc aaattcgcat gttttttatc tgaatcataa aatagtctaa
     2281 aatagctaaa ttattttttt cgcttggggt tttggcttgg ttggaattgg tattaaatga
     2341 gttgatcgaa cggaaattta tcccaatcat tacgtgtctc cagtatatct gtatttcatt
     2401 gaaaaaaaat taaatacatt tttacttgat ctgctttttc acaattgatg aacggtttac
     2461 atcccggttg catggcattt gaaatatgga gcttgatctg aatgggaaaa cacggcaggc
     2521 caagcaaggc agagcaactg cacatcgaga aaacactccg gtcatatttt gaaaagatgc
     2581 tgtctccatc atttgcagca agagatgctg gaatcaacat caatactgta aaaaaatact
     2641 atagggtttt ttcagatgaa atcagatcat ctgagcatcc tgactttatt cagagatcaa
     2701 aaaacatcat ccagaattgt agtctcgcat ttgatgttca gttatcaaag ctttacacac
     2761 tgcaagacaa gcttgaagca caaataaatc aggagatcaa acaaaaaggc aagattcctc
     2821 cagcacttta caaaatatca attcgtctat caaccgagat tgctggactg ttgttcaaaa
     2881 aggcaaacct gactcttagt ccgactgccg atatcacgct atctcgttac atcaaggaga
     2941 gtggaattgt tgcatagttc ttgggtatta aaacacgaca agcttgcaag aaattccttt
     3001 acccggataa agaaaagaca ctcaggtcat gccctgatca caccgccctc aaatcctctt
     3061 gaatggatag aaaaagtacg acccatagtg gaggggaaaa ccagaacatt tttggcctca
     3121 ccattctgga aggaaattta tcttgataat taccactcta agattatagt tggaggaagg
     3181 cagatcttca agtccaccta cattacggac attttggcat gcgaggcaac atctgcacct
     3241 ggaattcagg taggctatgt cacatttgat cagtccagcc ttaatggatt ctcaaagcag
     3301 aagctccaga ttggcacgtt ttcccaaaac cctgtcttgt cccgattccc taggaacaag
     3361 cttggaaaca ttggggagat ctcgcttcga aatggcagta ccatatactg catgactgac
     3421 aacaacgagt acatccacgt cgagggaaag tcgttaaacc actgcatctt agatgaggcg
     3481 caataccagg acatcgtaca tatagaaaaa atcaggcaga cgatgatggc gacaaaagga
     3541 aagctgtcaa ttcttggaat aggaggcgag tctggctctc cgtatgagaa attatggcaa
     3601 aactcagatc agagggaatg ggtatatgat gatcctaact ggcgggacag attgcagttt
     3661 gatggtgatg gactgattgc tgataaatat cttgtagatg tgctaagtgg aagatgggtt
     3721 ccgcagaaac ctgaaaacac aatgtatcat ggatatcaca taccgcagac gatatttgca
     3781 acaaatccgt tgaccattga tgatgcaata aacaaatacc gcatatctcc tttttactcg
     3841 attgaatacc agaaaaagaa caacccgaaa tcattttttt cgtcccacgt gatgggagaa
     3901 ttttacagat cagagaggcg gccaatcaca ccacaaatgg ttcattcctg catgaatcat
     3961 tacaggtaca ttggattgat gactttctct gagattgcag tgttgaaaga tgtgatgcag
     4021 gattcgatta atgtttccat gggagtcgac tttggttctg gaaatccgtc aaatactgtc
     4081 atatccattt tgattcactg gaaaaaatct gacaggatac agcttgcata cattgacaag
     4141 aggccggcag agcaccagct ggatcaggca gaacacatca acagattgta tagagcaagc
     4201 agatgcgaca ttggagttgg cgacttggga tacggcgcaa ttcaagttaa agtgattcag
     4261 gacggcggtt ccaacagaac aactggcaag aaatttcccg gagttgacaa cgagaacttt
     4321 tacggatgcc gaaacatcag caacgagagc aagccaatgc aggttcatcc taatgtgata
     4381 gacgagcacg gcgaggaaat tgagaggata tcaattgaca ttaccactgc aatccaaaag
     4441 ttcattgact tgattggaac atcaaaatca catcccagaa gagcagatga gtccagcagg
     4501 cgatccagac ttttgattcc gtttcatgtc gacagggaat acgagactga ctggctggtc
     4561 tcggacttta cgtccgttac aagaaaggat ctgggcagaa aggatctgga tccaagacag
     4621 aacgccaaaa aagagtttaa tcatcccaag gactcggtga tgagcataat ctatgcacta
     4681 gtaggccttg agcaagatac tgaatttcat tggattagcg cctagggtgt cttgcaagca
     4741 aaaggcaaga agcacaacat tcatggtcag gatgaattgc ttaggaattt gcagacagaa
     4801 aagatagatg atgcaagtgg tttgttaaga aaattaaaac aagttactgg aatgaaactt
     4861 caagtgttca tcactgacgg attaggatca tacgcagaag catatcaaaa ggaattttgt
     4921 aaaggagaaa caaggacatt agacacatca cttgcaaggt gatatgaaca acaagatgaa
     4981 gagattgaat agtgaatttc gtgacagaga aaaggtaatg cgatgaataa gattcagtta
     5041 cacttggtgg ttatcagttg tttcataatt acatcagaac ccacatggga ttagatggta
     5101 agacaccaag tgagaaatgc ggaactgaaa ttaatggtga taacaagtgg attacattaa
     5161 ttcagaattc taaacaaact aacatttcga gtatatgagg aaacagaagg aggatttttt
     5221 accagatgta ccgagtttct tggaatcata acacaaggag attcgaagga agatctaaag
     5281 agaatgatac tggatgcagt tgattgttat tttgattcat ttccaatgga gagagcaaag
     5341 atctctgagc taaaaaacaa caaaattgag attatttttt gattttccaa aaacatcatg
     5401 tgtaatgaat ttctaccgtc tttatccagg tccaattagc tgtcagtaat actttggttt
     5461 tttcaaattt caaaaataat tttgtttatt aacatacaat catagtacaa cttatgctgg
     5521 tgttagggga cagcaaagac tttgctccga tatacgaatc gttaccaaat aacgattcta
     5581 gaaaaacacg aattcatgat atcaaacatg cactagaaaa cgatcaaatt atcggagatg
     5641 ccattccatt tgataggtgg cccaaaaaat ttcgcagcga atttgactgc ttgtatcgcg
     5701 ttaggctatc agattctgat agattactct ataccattac agtagatgct aaaaccaacc
     5761 agaaaatagc cttgatgctc aaaataatgc ctcataagaa atatgaccga ctattcaaat
     5821 actaatacgt atatttatta tacatatatt ttatatactt atatgtacta tgaatagcat
     5881 acattggaaa cattagcaga tgataaaaaa aattcaaagc catatcttgg aattgtggga
     5941 tatcttgatt ttctgttaga agctacaaaa aaggtcaacc ccacactacg aaatgtcgcc
     6001 accgtagcca agataatctc cgaacacaaa tactttgaga caaaaagcca gctgaacaaa
     6061 aaactgccaa aggcaatgca gtatccaaca tttaacttca tactggatta tctgcaaaaa
     6121 acaaacatga tacagctaaa tcctgacggc tcaatagtgt ggatctatcc tactagtcag
     6181 aaactaaaga agagaatcat ccaggcaaag ccactttagt tttgtgtacc atacaagata
     6241 ttttctgcca tctgtctctt cagaaactgc ttcaaaatac ccagtatacc gtatggatta
     6301 caggtgtaag atcttgcaaa gataggacaa gaaagcccaa cactctttag cgttgggatg
     6361 agtcacagtt tcattatttc tgatgtattg tagggttttg agacgagttg tagatacctc
     6421 aattgtgcgt gatttcgtta tacttatgta acgataatgt tgttaggtga cattatggtt
     6481 acaaaactaa taagactcgg ttccaccgag ttgaagctga tactcactct ggaagaggaa
     6541 gggaaaaaga tattcaatac tgctgatgcg tatagaattc tcaagtcgtc aaaggattcc
     6601 gtaaacgctg ctctctatag actcagaaaa aagggaagaa tagaagagat agagcgcggg
     6661 aaatatcttc tgatacctgc aaaggcaggt tacagtggca aatgggcaga agtacctttc
     6721 atcatagcat cggaaattgt ggatccttat tacataggat ttgcgtcggc tctcaattac
     6781 tggggtatga cagaacaggt tccaagtaca acttttgtgg tgacaacaaa gagaaagaga
     6841 aatctggaat acggatcact caaattcaaa tttgttacgt tttcaaaaaa gagattcttc
     6901 ggcatagtcg aagaagagat cgccggagag aaattcaaaa tatcatctag ggagaaaaca
     6961 attgtagacg gtttgaacta tccgagatat tgcggcggcc tcgatgaaat agtaaaaggc
     7021 atttgggaat ctcaggatga aatcgacttt gcaaagatca tcgactacgc aaaagagatg
     7081 agaaacgatt ctgtcaagag aagattattc tacatactag acattctaga attaaagaag
     7141 aaagtcagcg taaaagatct gaacaaaatt ccaaaaggac taaagtggtt agatccaagc
     7201 ggtccaaaaa atgcaataga atattccaaa gagtatggtt tgatcataaa caggactaag
     7261 aaggacttga tgagctggag gggatactag atgttaagta aagacgacat aatgaaaatt
     7321 gccggcgaga aaaaaatcaa cccgtcagca gtgagcaagg attatgcttt aggatggttg
     7381 ttatttggca tttcaaaaag ctcgataggt aacaagctta ttttcaaggg aggtactgcc
     7441 ctttccaagg tgcattttcc tgaagggtgt gggctttctc accatgtatt tgtaaaattc
     7501 aagttatagg cggataaaaa ataagatata aaccgatacc ggttaaaatc aatccggcga
     7561 tagttagtcc gattccaagt acaggattcc atttttccca tttagttttc ttatgattgt
     7621 aatttactgt gaaaacataa ctgtcatgta tatctgcacg aatcttatgt gccatatctt
     7681 gtaaattaga attttcatct atggtaagct catcccaaat aactatttga tatggactat
     7741 ttcttggact ctctatacga taacttggtt tgtatgaatg aaaaaattgt tcgattattg
     7801 gtcttacttc ggtttcagga tcacatgtat ttggacatac tacacctata gatgcaatcc
     7861 tcatatggaa ttatgaaaat atgatggtaa tgaagctttt attttattat taatttgatg
     7921 tcacaaacat cattacctgc ttgtgtgtac agttgatttt ttcttttaat agcatctgca
     7981 acagaatcaa atttccttgt accaaagtga tcataaacat gatccttttt tgcatctttt
     8041 atactagaaa aatcattcat tagagttttt acccgattac atgcaatttc tgtattagca
     8101 cataacactc tgaattctac ttcaggcatt gatgaacatc tctttcactt gcttattatc
     8161 gttatcaaga taacatcgtt ctagaatcat gtgtgttata ctgcacattt caaacatata
     8221 agtaatatct aacttatccc gaaactacga ttttcacttc aaaatcatga atttgagcca
     8281 aaacaaatga attaatcatc aattatttag tccaattttc ggtgtttttc aagttttggg
     8341 atgagttgat catatcatat ataacatata catatcacta gtctaacata tacataacat
     8401 atatactagg ttatgtataa tatacatatg acacaagtag gacagagtat cagttttagt
     8461 gaggagttaa ttacagcaat aagagtttta gcttcatcta aaaaacaacc actttcacaa
     8521 tttttagaat ttcaattaag ggaagtatca atcataaaag aaaccatacg caaactcgaa
     8581 aaaatccccg atttacctcc aatggtgaaa gggagtattc cttattccac agtagaagaa
     8641 atctaagtca gaattttttc cataggtgtt aagaaatcat aatgtggatc ggatcttcta
     8701 gcaccagtga cggtatactc gtgtacgtcc tgataatatg ataaacgaga atctgttgaa
     8761 tacaaagtga ttttaggata accaaatgtc aatgataaat cttccagttt tttgatcagt
     8821 tgatatcctg gtttgtgaat agcggtttct ttgtgtttcc tattattttc aacaagatcc
     8881 aagtgaaaat gggttccttt gtcaataaat gtaagaaatc caagtatctt accttgataa
     8941 tcatacacaa ggacatatcg acttgaaatg tttttgccaa ccaagtaaga taaatcaata
     9001 ttattttgag gttggatttc attcgtaaaa atttgttcgg cgtcttgtcg acatatttca
     9061 cgaaaaggtg attctaaatc gagatttttt ctttgttgca tgtcttaaac tagtgattca
     9121 taatttgtgt tttaaacttt agatattcaa ggctacctag aaatcatcga cgacaaacaa
     9181 gaaattatag tcattctgtg acagttgtag gcaagtgccc tgtacagcgg ctctttttct
     9241 ttcatgatat catggtatga ttttatctca gatccaaact ttctttttat cacactggat
     9301 gttgtctcgg atttgcttcg ctgatgtagc aactgcactg gaagcattct ctttcctgtg
     9361 aattcctcag gagggatgat ttgtgtcaca actgtatcag aaacagcata tcatgtcaat
     9421 tcatgttttg acgtgaaatt gaattgaatg aaaaatcttc catgtacgaa tgtcactgcc
     9481 aggttaattg tttagaagtg tcattgtttt taatgattcg ttagtgaggt ttaattcttc
     9541 acgtaataat t
//
LOCUS       DASWKH010000047         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_477981, whole genome shotgun sequence.
ACCESSION   DASWKH010000047 DASWKH010000000
VERSION     DASWKH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477981"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..429)
                     /locus_tag="VGA92_03525"
     CDS             complement(7..429)
                     /locus_tag="VGA92_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX9845517.1"
                     /translation="MGKVIYDYKDWDEIEGCVDRLVKKILSRDLHFSKISTISRGGLV
                     PARLVADRLNIKQILVDDETIPADSLFVDDIYDTGETFRKIIERADDPDKLVYATLFA
                     RRKPRFPKQLVYARLTRNTEYLVYPWDRFEHGIPYESH"
     gene            542..976
                     /locus_tag="VGA92_03530"
     CDS             542..976
                     /locus_tag="VGA92_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9845518.1"
                     /translation="MFTLRKILVPLDGSDNSERALDAAIFLAKKSNAKIICLYSINFI
                     PVAETQMFDPIRCQVEEKKYAEKVMQKAKATCTQNDVEFTKMIEFGMPGNTILNFIKN
                     KNNKIDLVVMGSRGQSGLKEIFLGSVSNYVLHKSPIPVLIIK"
     gene            1023..1784
                     /locus_tag="VGA92_03535"
     CDS             1023..1784
                     /locus_tag="VGA92_03535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9845519.1"
                     /translation="MRLKDKIAIITGASSDIGADMSKRFVEEGAIVIMLGRNINSLEK
                     ARSTIKNKESAVSMTCDITDDSQILSVIEQVVDKYGRIDVLVNNAAKINDPIHFHEMK
                     DSDMMDLVNTNIVGVFKMTKAVLGKMMDTKSGCIINIGSISSERAIPRVHLAVYSATK
                     ASISMFTKSIAVEYARRNIRCNCLNLGIINAGMIKPYLEDPQARKVLEDRQPINRIGE
                     PSDVSSAAVFLASDDAKWITGTILNIDGGKSASEG"
     gene            complement(1823..2683)
                     /locus_tag="VGA92_03540"
     CDS             complement(1823..2683)
                     /locus_tag="VGA92_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845520.1"
                     /translation="MKLKNRSLDQVIRNAITVTPNLTLLEAREILLKYKVGRLPVIDA
                     GKRPVGIVTEKDFVRAIYEHSEKSVEKVLVKDFMSKNLITVTRNDAIHKCAKLMRQNK
                     VSSVLVLDSDRTLAGIVTKTDLASVFLTHSVGPLLVSDIMTSKVITVMPADSLLLVEN
                     LLVKYRISRIVVERNKKPVGIITNRDFLPAKMPRWIRQFADPKEVEKYRLNPKPDEFR
                     MNQLSYILSFRAEDIMTPNPITVGANEDVSAVALLMIRNGISGLPVVKNSLLAGIITK
                     SDIVRAIAEM"
     gene            2783..3928
                     /locus_tag="VGA92_03545"
     CDS             2783..3928
                     /locus_tag="VGA92_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845521.1"
                     /translation="MSIDVIHEHMNQIMKTKIGTIITKPILIEPTAKASQAITMLSEA
                     DVFDAFCRHNGSTLNVNIRDLLQSKDISHMNIGAFLHPIQSLSENDTIENAVTIITHN
                     RTRAAPVIRDDEVIGVVEAKNILKLISELDNKWIKANQIFTPNPIIINKQTPLGSARR
                     TMVSKRIDHLPVIHKDKVSQVLTSYHLLQMILPAERVGKRDIGSKKIRSLESQVGNLG
                     TNRMASCSPLDNLNQVIDSMLHANTTFCLVLLRTGLQGIITYRDVLNLLVTKQKSTIP
                     LFIVGMPKEDNASIITSKFTKVLDRLAKVYPDIQEARVYVKKVHGVESRYNYEVSTVI
                     LTPIKRYIFARNGYDLSKIFDEISGRILRNLAKRAKNRYKFSIRKMM"
     gene            3994..>4242
                     /locus_tag="VGA92_03550"
     CDS             3994..>4242
                     /locus_tag="VGA92_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9845522.1"
                     /translation="MKKTSKNQQNGNILLLGFPGNGLIGTFTISYLISHLKMRPIGEI
                     NHPELPPTLFVENGEIIGPIRIYKKDNLYAIISDIPIYP"
BASE COUNT         1423 a          826 c          758 g         1235 t
ORIGIN      
        1 cgtgaactaa tgtgattcat atggaatacc gtgttcgaat ctatcccaag gatacacgag
       61 atattcggtg ttcctagtta gccttgcata aactagctgt tttgggaatc tcggttttct
      121 tctagcaaac aatgttgcat acactaattt atcaggatca tctgcacgtt caatgatttt
      181 tctaaacgtt tccccggtat cataaatatc gtcaacaaat aatgaatcgg caggtattgt
      241 ttcatcatcc acaagaatct gtttgatgtt taatctgtct gctacaagcc ttgccggaac
      301 taaacccccc ctgcttatag ttgaaatctt actaaagtgt agatctctag atagaatttt
      361 ttttactagc ctatcaacac aaccttcaat ttcatcccag tccttgtagt cgtaaatcac
      421 tttccccatt acaatgttgt ccgatataca tgattttatc ctactgattg attatcagaa
      481 ctgaaattca aatctgataa tttgactgcc atttatgtat ataaaataaa aactatgatc
      541 attgtttaca ttacgaaaaa tccttgtacc attggatggt tcggacaact ctgagagggc
      601 cttagatgct gctatttttt tggcaaaaaa gtctaatgca aaaattatct gtctatattc
      661 gataaacttt attcctgttg ccgaaaccca aatgtttgat cccatcaggt gccaagttga
      721 agagaaaaaa tatgcggaaa aagtgatgca aaaagcaaaa gctacctgca cccaaaacga
      781 tgttgagttt acaaaaatga tagaatttgg aatgcctgga aacacaatac tgaattttat
      841 caaaaacaag aataacaaaa ttgatctggt ggtaatgggc tcacgaggac aaagtggcct
      901 aaaagagatc tttttgggaa gtgtctcaaa ttatgtgtta cacaaatctc caattccagt
      961 attaatcata aaatagtata aagcagcaca agtcatttaa gatattccat ggtagggctg
     1021 atatgcgatt aaaggacaaa atagcaatca taactggtgc atcaagtgat atcggcgccg
     1081 acatgtccaa acgatttgta gaggaaggtg ccatagtcat aatgcttggc agaaatatca
     1141 actctctaga aaaggctcgt tctactataa aaaataagga atctgcagtt tcgatgacgt
     1201 gcgatattac tgatgattcc caaatactaa gcgttataga acaagtagtc gacaagtatg
     1261 gcagaatcga tgttctggta aataacgctg caaaaatcaa cgatccaatt cattttcatg
     1321 aaatgaagga ttctgatatg atggatctag ttaacaccaa cattgtaggt gttttcaaaa
     1381 tgacaaaggc agttcttggt aaaatgatgg acacaaaaag tggttgtatc attaacattg
     1441 gctcgatctc aagcgaacgg gcaatacctc gagttcacct agcagtgtac tctgctacca
     1501 aggcatcaat ttcgatgttc acaaaatcaa ttgctgtaga atatgctaga cgaaacatac
     1561 gatgcaactg tcttaatttg ggtataatta atgcaggtat gataaagcca tacctcgagg
     1621 atcctcaagc gcgaaaagtt ctggaagata gacaaccaat aaacaggata ggtgagccat
     1681 cagacgtatc aagcgcagct gttttcttag catctgatga tgcgaaatgg attactggga
     1741 caatcctaaa cattgatggc ggcaaatcgg cctcagaagg ataatctttt ccaaattttc
     1801 ttaattcaat aatttgtaat tactacatct ctgcaatcgc tctaacgata tctgattttg
     1861 ttattatgcc agctaatagt gagtttttga caactggaag gccactaatc ccattcctaa
     1921 tcatcaaaag tgctactgca gagacatctt cgtttgctcc aaccgtaatc ggatttggag
     1981 tcatgatatc ttctgcccta aatgacaaaa tgtaagagag ctgattcatt ctaaattcat
     2041 ctggctttgg attcaatcta tacttctcta cttcttttgg gtcagcaaac tgtctaatcc
     2101 atcgaggcat ctttgctgga agaaagtccc tgtttgtaat tatgccaaca ggttttttgt
     2161 ttcgctcaac aactatcctt gatatccgat attttacaag aaggttttct acaagcaaca
     2221 aagaatcagc gggcatgact gtgattacct ttgaagtcat tatatcggac actaaaagtg
     2281 gtccaactga atgggtcaaa aatacggacg caagatctgt ttttgtcaca attccagcaa
     2341 gggttctatc gctgtctaaa accaagactg agctaacctt gttctggcgc atcagtttgg
     2401 cacacttgtg aatggcatcg tttcttgtta cggtaatcag gttttttgac ataaagtctt
     2461 taacaagaac tttttcgacc gatttttcac tatgttcgta gattgcccga acaaagtctt
     2521 tttcggttac tatgccaact ggccttttgc cggcatcgat tactggtaat cttcccacct
     2581 tgtatttgag taagatctcc cttgcctcaa gaagagtcag gttgggcgtc acagtaatgg
     2641 catttctgat cacctggtct aagcttcgat tttttaattt catactttat ctgccttttc
     2701 aattgttaaa tattcaaaca agaatatcaa gcttgaaaat tatactattt atttaactca
     2761 acaaaataca gcaatatagc aaatgagcat tgatgtaata catgagcaca tgaatcaaat
     2821 catgaaaaca aagattggta ccataataac taagcccatc ttaattgaac ctacagctaa
     2881 ggcctcacag gcaataacca tgctttctga ggctgatgtc tttgatgctt tttgtcggca
     2941 taacggttcc accttaaacg taaacatacg tgacttgctt caaagcaaag acattagcca
     3001 tatgaatata ggagcatttt tacatccgat acaaagtctt tcggaaaatg atacaattga
     3061 aaatgctgta actatcatta cccacaatag gacaagggca gctcctgtta taagagacga
     3121 cgaagtcata ggtgtagttg aagcaaaaaa cattctaaaa ttaatttcag aacttgacaa
     3181 caaatggata aaagcaaatc agatctttac accaaatcct ataattatta acaaacaaac
     3241 ccctctaggt tctgcacgaa gaacgatggt tagtaagaga attgatcacc ttccagtaat
     3301 acataaagac aaggtctctc aagttcttac ctcgtatcac cttcttcaaa tgattcttcc
     3361 agctgaacga gtaggaaaac gggatattgg atcaaaaaag attcgtagcc tagaatctca
     3421 ggtgggaaat cttggtacaa atcgcatggc aagctgctcc ccgcttgaca acctaaatca
     3481 agttatagat tcaatgcttc atgcgaacac aaccttttgc cttgtcttat tgaggactgg
     3541 cttgcaagga ataatcacat acagggacgt tctcaacttg ctcgtaacaa agcaaaaaag
     3601 caccattcca cttttcatcg taggaatgcc aaaagaggat aatgcaagca tcattacaag
     3661 caagtttacc aaagtacttg atagactagc aaaagtatat cctgatattc aagaagccag
     3721 agtctatgta aaaaaggtgc atggagtgga aagccgatac aactatgagg tctccacagt
     3781 catactgaca ccaattaaaa gatacatttt cgctagaaat ggatatgatc tcagcaagat
     3841 ctttgacgaa atatctggaa gaatacttag gaatctagca aagcgagcaa agaaccgcta
     3901 caaatttagc atcagaaaaa tgatgtaaca ttcaattatc acatctgaaa aaagaaaggc
     3961 tgcttttatt tgactaaaca caagtacaat caattgaaaa agacttcaaa aaatcaacaa
     4021 aatggaaata ttctgttgtt agggttccca ggaaatggac taattggaac ttttaccatc
     4081 tcatatctta tatcacacct gaagatgagg ccaattggag aaatcaacca tccagagcta
     4141 ccaccaactc tgtttgtgga aaatggcgaa ataattggac caatcagaat ttacaaaaag
     4201 gacaatcttt acgccataat ttctgatatt ccaatttacc ct
//
LOCUS       DASWKH010000048        15161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2340074, whole genome shotgun sequence.
ACCESSION   DASWKH010000048 DASWKH010000000
VERSION     DASWKH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340074"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1174
                     /locus_tag="VGA92_03555"
     CDS             2..1174
                     /locus_tag="VGA92_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9845523.1"
                     /translation="MIYVLVAWVAILGVAKALKLDKHGFAIKPYSLTYKNTQVQMALT
                     KMLGRTRRGIRVFADVSVIAGFIMMGFAFWFLLDNVVKYFDRPTEFAELTVLIPGVTL
                     TSSSAILYFFLSIPIVLVIHEGAHGIVATLEKIRIKTGGFAIFIAMFAGFVEPDDEEF
                     NKAKKISKLRVIGAGATANVIFALALGAVLLTNPFFAIIVPEPLRSAFYEIPDGVSIL
                     SIMPGTGAEKAGLQPNDIITSINDVRITTPFDFTKVDLAPGDTAVVSVLRDGASQQFS
                     VEITPSPDDPTVGLIGIMRDNSISYKPVYNFIEWNSPELSLFLLWLWMISFFIGIINM
                     LPLPILDGGKFIHSIIDKKLSDVAVNRTMWAIYAFTFCLFGLNIALSYMKSGWFTI"
     gene            1220..2125
                     /locus_tag="VGA92_03560"
     CDS             1220..2125
                     /locus_tag="VGA92_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HEX9845524.1"
                     /translation="MKCDRCQNTAVYSRKYSGENFCSECFSNSILRKAAKTISKYNMI
                     QNGELVCVAVSGGKDSLALLNVLSKMSKNHNFGIHAITIDEGIAGYREEALEIVKKFC
                     TSLGVSHSVYSYKELFDQTLDESLKLREDEKISSCSICGTLRRRAMDHAAKDIGANVI
                     ATGHNLDDTLQTFIINTLSGDTNKIGWMDPDTSDNSLRKVKPFCEIYESEIVFYAFTN
                     GLPFQTEPCPHMNEGIRTEIREFLNKLENSHGGIKNSMYRSVLKISQVMRESNYKERT
                     KCTNCGAECTGKICSVCKMLVDLRK"
     gene            2159..2472
                     /gene="ffs"
                     /locus_tag="VGA92_03565"
     ncRNA           2159..2472
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGA92_03565"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            2521..2850
                     /locus_tag="VGA92_03570"
     CDS             2521..2850
                     /locus_tag="VGA92_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845525.1"
                     /translation="MDDLFLGPKRTYEDFKKQIPTDLLPVFDSLREFCLSLGSNVVQD
                     IRAHRVVFGKSLSFRWFADLEPDQQGIIVKIQRDRKEPFNTISIKPDQDSDELKKLIL
                     DAYNTIR"
     gene            complement(2847..3125)
                     /locus_tag="VGA92_03575"
     CDS             complement(2847..3125)
                     /locus_tag="VGA92_03575"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9845526.1"
                     /translation="MVKQRVHLFIKGKVQGVFFRQAMKVAAKKNRVSGWVKNLKDGRV
                     EAIIEGEDAGVSNMVEWCHAGPANARVEDIEIRNEKFKEEFAKFEVLY"
     gene            complement(3185..3916)
                     /locus_tag="VGA92_03580"
     CDS             complement(3185..3916)
                     /locus_tag="VGA92_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX9845527.1"
                     /translation="MGKRPLVRRRGRGGMQFRAASTGKLAPAKYPHFDLSEDHTGTVI
                     DLIHERGRDAPLAKVRFEDGSVSLVPAILGTKIGSTVKFGLNAEITNGNVISIQNIPD
                     GTIVCNIEKHFGDGGAIAKTAGNSATVFSHGVDGVTIKLPSGSFTTLNPKNRAMVGIL
                     AGGGVSDRPFLNAGNKWRRYQSKGHKYPIVRGVAQAAYAHPHGGGRHQHMGQSGTVSR
                     NAPPGAKVGSIAARKTGRARLKERV"
     gene            4010..6214
                     /locus_tag="VGA92_03585"
     CDS             4010..6214
                     /locus_tag="VGA92_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX9845528.1"
                     /translation="MSQSALSLKVLEAYTRDVGRGVARIDYDSMDTLNASTGDVIEIK
                     GKRRTVAKCLPLYPSDEGKGIIRIDGLGRNNAGIAIGDTISVRKIKAVAAEKVVVAPL
                     EAIPPIDERYLADALESVPLIKGDNVMVPYFGGRLTFQVIGVTPAADAVLVTQKTVFH
                     IAEKGETLRGVPQVTYEDIGGLTDEIKKVREMIELPLRHPEIFEKLGVEAPKGVLLYG
                     PPGTGKTLLAKAVANESNAHFISISGPEIMSKFYGESEARLREIFKEAREKSPSIIFV
                     DEIDSIAPKREEVTGEVERRVVSQMLSLMDGLEARGKVIVIAATNRPNAIDPALRRPG
                     RFDREIEIKVPDKKGRKDILNIHTRNMPLVTDENDSNYVNVDKISAVSHGYVGADLEY
                     LCKEAAMKCLRRLLPELTLEDEKVPPETLDKLIVNNDDFQKALIEITPSGMREVFIEN
                     PDVKWDDIGGLKEVKQQLQEAVEWPMKYPGLYDKLGHKMPRGILLHGPSGVGKTLLAK
                     AVATESEANFVSVRGPELLSKWVGESERGIREIFRRARQASPCVIFFDEVDSIAPIRG
                     AGSETAVTERVVSQLLTELDGMENMHGVIVLAATNRADMIDPALLRPGRFDKIVQIPM
                     PDKDSRRQILQINAKDIPIVSDKNDPGYVNLDKIAEITDGMSGADVAAIGNTAVSLVI
                     HEYLDKHPTKEELDNAASNAKVTMRHFEEAVKKVKTQKDLKIGQKIAVPYYR"
     gene            6249..7532
                     /gene="gatD"
                     /locus_tag="VGA92_03590"
     CDS             6249..7532
                     /gene="gatD"
                     /locus_tag="VGA92_03590"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HEX9845529.1"
                     /translation="MSEYRGYSGRALEFLKQNKVKVGDTIKITTDSDYTGTIMPRYEH
                     TDDKHIVLKFKNGYNVGLEITQVKKVEYISTIEPNQEKNTTTVFNPSLPKVLLLSTGG
                     TIASRVDYRTGSVTPALSAQDLNASVPELSEIANIDAEVLFSEYSENLTPEHWIKIAQ
                     RLDSLAKSDYKGIIVAHGTDTMQYTAAFLSFSLAGFPKPIVLVGSQRSSDRPSSDAAT
                     NLISATRFIVNCKVNGVFVAMHNTESDDEIACHLGTRVRKNHTSKRNAFETMGGKPAF
                     IITQNEIIQNFSGSYFKEAQYTPNIKIDTKVALIKYHPGYDPKLVDEMINLGYRAIIF
                     EGTGLGHIGKTMYDAIKKAREKKIFLGMTSQCLDGRVNMAVYESGRDLLHLGIVPLSD
                     IISETALVKAMWAVATHDKNIEEIMLKNIASEITE"
     gene            7563..9458
                     /gene="gatE"
                     /locus_tag="VGA92_03595"
     CDS             7563..9458
                     /gene="gatE"
                     /locus_tag="VGA92_03595"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HEX9845530.1"
                     /translation="MSEIQPDKIGLKVGLEIHQQLATNKKLFCGCRPVESAEFPIRFA
                     RKLRLTKSELGKYDPAAMFEASKSKMIYYNANPESSCLVEYDEEPPHSLDSDAKLSAL
                     IFASALHSNVFNEGYVMRKIVIDGSNTSGFQRTMLVSQGGYIDVEGNKIGVQTICLEE
                     DAAKLLKDNESTREYALDRLGIPLVEIALEPVSGSPAQVKKIALTLGRLLRVTHRVAR
                     GLGSIRQDVNVSVSGGGIVEVKGVQQLDQLEKVIRYEAKRQHGLQLIAEKLQKINLDS
                     ISKDDVLDVTPQFASCKSKIIQKSLKDGAAIKALRIKNFAGMFGYEPYEGIRLGRELG
                     QQVRFFGIGGVFHSDELPNYGIEQSDVDQLRDILKLEPNDGFLIISGSKEKIDFAVDA
                     TINRIKNAKNGVPAETRAATPTGETVFLRPRPGSSRMYPETDIPPVIITDEEIAFAKN
                     NIPRSWDETILELQKKYGINAQLAEQIFDSDYLDLFESICAYKKNNPNFVASILCSTI
                     TNLQRQGLDSSLLKAEHILRSFELLAQDKITKESLEMIFESIMSSKIRSPDEFVQKHS
                     GSFDENELEQILDELIKKNLEMIQKQGLHSTGFLMGLAMKTLRGKVSGEKISKLLESK
                     ITKIIEK"
     gene            complement(9478..9732)
                     /locus_tag="VGA92_03600"
     CDS             complement(9478..9732)
                     /locus_tag="VGA92_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845531.1"
                     /translation="MGKIVKVGGRGTSRRVAESEDETWAGEKFKEYQKKMKEKAKDEY
                     VVSGRGTGKRKLGDTPETTRPSAKGRYVSSGRGTGRRKLD"
     gene            complement(9792..9876)
                     /locus_tag="VGA92_03605"
     tRNA            complement(9792..9876)
                     /locus_tag="VGA92_03605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9838..9840),aa:Leu,seq:cag)
     gene            complement(10120..11205)
                     /gene="dnaJ"
                     /locus_tag="VGA92_03610"
     CDS             complement(10120..11205)
                     /gene="dnaJ"
                     /locus_tag="VGA92_03610"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9845532.1"
                     /translation="MSAKRDYYEVLGVSKTDSVDTIKSQYRKLALKFHPDRNKSPEAA
                     EHFKEISEAYAVLADPEKRKVYDQYGHEGLGGRYSTEDIFQGARGNFDDVFSNFGFGS
                     FDSIFESLFGRSGGFGFARQKGQDILYETSITLEDVFSGKRLEIDVKKNIDCNNCKGS
                     GCAPGTSSETCPNCRGQGQIRITRNMGFSTFVTVQPCTKCRGEGKFIPKPCSNCKGTG
                     KTKGTKPLSFDIPPGIDNGDYVISGEGEPIRGGINGDLIVRIRVKPHQFFKRDGADIF
                     YDAKISIVDACLGKTIEVPTLEKSEKVKVEEGTQPNTIIKLKGKGLPYSGSRGRGDQY
                     VRFVVEVPSKLSKHQKELLQKFGQALE"
     gene            complement(11242..13104)
                     /gene="dnaK"
                     /locus_tag="VGA92_03615"
     CDS             complement(11242..13104)
                     /gene="dnaK"
                     /locus_tag="VGA92_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX9845533.1"
                     /translation="MAKIIGIDLGTSNSAAAVVMGGKPVLIPAAEGTSIGGKAFPSVV
                     AFTKDGQLIVGEPARRQAITNPDNTIVAAKRKMGSDYVFKIQGKDYKPQQISAFILQK
                     IKKDAEAFLGEKIDKAVITVPAYFDDNQRQATKDAGTIAGLDVVRIINEPTAASLAFG
                     LDKVGQDMKILVFDFGGGTLDVTIMEMGGGVFEVLSTSGDTQLGGTDMDKILIDYVVN
                     EFRKTTGVDLTKDSTAMTRIREASEKAKIELSTVMETDINLPFLSYDQSAGPKNLEIK
                     LTRAKFEELIRPIVEKCRGSVDNALRDAKLAPSDVSKIVLVGGPTRIPLVKKFVGDVI
                     GKSPESGVDPMEAVAVGAAIQAGIIAGDVTSDIVLLDVTPLTLGVETLGGLREPLIER
                     NTTIPTSKTKIFTTAADNQTAVTIHVVQGERPMAADNVSLGSFNLTGLPPAPRGIPQI
                     EVKFDIDANGILNVTAKDLGTKKEAKITIQAGSKLSKEEIEKLKQDAEKFADEDKKKK
                     ETVDIKNEAESFVYTAEKLLTQDLKDKLTQEQGIKISEAIKELKEVLDKDSEQIRPKL
                     DALKTIVNQISTELYKNAAPPPNTEEQQSQSGPSDQNTQDGTADQKTQGTGTTS"
     gene            complement(13110..13691)
                     /locus_tag="VGA92_03620"
     CDS             complement(13110..13691)
                     /locus_tag="VGA92_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9845534.1"
                     /translation="MSENNNDENKLENSQSLPTEEDLEIVAEPKSADQLIKELDNKII
                     ELQQSLVSCEDKLKRSLADFVNLERKTKSDIENGVNQKLDKFMITFLTIYDDLVRAKE
                     ILKKENPNARGLDFILKNIEALLSEYGIAPINALGEIFDPNLHEAISVMEDENLDENT
                     ITKEIRKGYISHKRTLRPTLVEISKKPQQNNLK"
     gene            complement(13720..14862)
                     /locus_tag="VGA92_03625"
     CDS             complement(13720..14862)
                     /locus_tag="VGA92_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845535.1"
                     /translation="MVADRVVVYGLSTEGYAIACQMAIRGANVFIIDESSQSAISLKA
                     EIAKTYPNVSSLKEDEPLLAMEPIDVAISKAQYLFFAPRIRKIGQETKTEVHSKFKDA
                     TSSLKKGSSVIYILPTGIGGNGENISLLEHVTGLEVGKSIFYYYYPLGGGNQPPKVIG
                     SYNSKQDQSLANLLTGNKKEISFVGISSAEHFHAINVLSRFSSLCSVLEVCKFAQDDN
                     TKTDLQSDDFQNIFLDDMVNGLYDLRSLGTSFEGVNTLMYLINGSLKGIDGYIKRLID
                     EIRTTLKKNDLKASRTKIALSWSLDQHEMRGDKIEMLQNLITKLRDYIGDVEAFADPN
                     LDLFHNDKTTILVICSKSDYERLHKKYDNDLIIIKGNPLCEILHKD"
     gene            14928..>15161
                     /locus_tag="VGA92_03630"
     CDS             14928..>15161
                     /locus_tag="VGA92_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX9845536.1"
                     /translation="MKIWFDILTPKQILFFEPMIKQLQKSNQILCTSRNYREVTHLAK
                     LRKMKIALIGKHGGQSKIDKLNASLSRMTALARQ"
BASE COUNT         4361 a         3128 c         3218 g         4454 t
ORIGIN      
        1 tataatttat gtcctagttg cgtgggtagc aatactagga gtagccaaag cactaaagct
       61 agacaagcac ggctttgcca taaaaccgta cagcctcacc tacaaaaaca cgcaggtcca
      121 aatggccttg acaaagatgc ttgggcgcac gcgacgcgga atacgcgtat ttgctgatgt
      181 tagcgttatt gccggcttta ttatgatggg atttgccttt tggtttttgc ttgataatgt
      241 ggtaaaatat tttgataggc ctacagagtt tgcagagctg actgttttga ttccaggcgt
      301 tactttgact tcgagttctg caattttgta tttttttctc tccattccaa ttgtcctagt
      361 aatacacgag ggggcgcatg gaattgtcgc cacacttgag aagataagaa tcaagactgg
      421 tggctttgcc atcttcattg caatgtttgc aggctttgta gagccagacg atgaggaatt
      481 taacaaggca aaaaagatct caaagcttag agtcattggt gcaggtgcga cagcaaatgt
      541 gattttcgca cttgccttag gtgccgtact tttgacaaac ccgttttttg caattatcgt
      601 gccagagcca ttaaggagcg cattctatga gataccagat ggcgtttcca tactatcaat
      661 aatgccggga actggtgcag aaaaggcagg gctgcagcca aatgatatca tcacatcaat
      721 taatgatgtt cggataacta cgccgtttga ttttacaaag gtagatcttg cacctggaga
      781 taccgcagtt gtatcggttc tcagagatgg tgccagccag cagttttcag tagagataac
      841 tccttctcct gatgatccca cagtaggatt aatcggcata atgcgcgata actccatttc
      901 atacaagcca gtctacaatt tcattgagtg gaatagtcct gagctgtcat tgtttttgct
      961 ttggttgtgg atgatatcat tttttattgg aatcatcaac atgctgcccc tcccaatatt
     1021 ggatggcgga aaattcattc acagcataat tgacaaaaag ctctcagatg ttgcagtaaa
     1081 cagaacaatg tgggcaatct atgcgtttac tttctgcctc tttggcctaa acatagcgct
     1141 ttcctacatg aagtcgggct ggtttacaat ctagtttttt ttcaaatatc gttaaatttg
     1201 ggcttgaaaa accagattca tgaagtgtga cagatgccag aacactgcag tttattctag
     1261 gaaatattcc ggagaaaact tttgttctga gtgtttttca aactcgattc taagaaaggc
     1321 tgcaaaaacc atctcaaaat acaacatgat acagaatggt gagctggtat gtgttgctgt
     1381 gtctggcgga aaggactcgc ttgccttatt gaacgtatta tcaaagatgt caaaaaacca
     1441 caactttgga attcatgcga taacaattga tgagggaatt gccggatata gagaagaggc
     1501 gctggaaatt gtaaaaaaat tctgcaccag tcttggtgta agccatagcg tatactcgta
     1561 caaagaactc tttgatcaga ctttggatga gtcattgaaa ttgcgagaag atgaaaagat
     1621 ttcttcttgt tcgatttgcg gcacattaag gaggcgtgca atggatcatg cagcaaaaga
     1681 cattggtgcc aatgtcatcg caacaggcca caaccttgat gatacgctgc agacttttat
     1741 cataaatacg ctttctggcg acacaaacaa gattggctgg atggatccag acacttcgga
     1801 taactcgctg cgaaaagtaa agccgttttg cgaaatttac gagtctgaaa ttgtgtttta
     1861 cgcatttacc aacgggttgc catttcaaac agagccctgc ccgcacatga acgagggaat
     1921 tcgaaccgag attcgtgaat ttctaaacaa gttggaaaac agtcatggtg gaataaaaaa
     1981 cagcatgtac cgttctgtct tgaaaatttc acaggttatg cgagaatcaa actacaaaga
     2041 aaggacaaaa tgcacaaact gcggcgctga gtgcactgga aaaatttgct ctgtgtgcaa
     2101 aatgcttgta gatctgagaa aataatccaa atatagcatt agtttgtaat ggtatgtggt
     2161 agtaggtagg atcggggcgc tagccgtgcc ccattctgaa accggctcta tgcagagatc
     2221 agtaactgct ggataaatct ctaggtggat aattcccgct tgatgtactg aaatgaaatc
     2281 acgccgaagc gggtggttgc aggtctgaga ttgtccgaag ggataactct ttagaccgag
     2341 ttgccgaaat ggatgaggtc cgggagggag caatcctaag gcaaagcatc cacgctactt
     2401 cgtcaacgtg gcggatcttg tgcatcttga gatggggcta cctgtctaga ctggaactag
     2461 caggtctact accacttgat ctttttcaaa aatatatagc attaacaaaa agaaatacac
     2521 atggatgatt tattcttagg accaaaaaga acctatgaag attttaaaaa acaaatccct
     2581 actgatttgc tacctgtttt tgattcacta agagagtttt gcttgtcttt gggcagcaat
     2641 gtagtacaag acattcgtgc acacagagtt gtttttggca agtcactttc gtttaggtgg
     2701 tttgcagatt tagagccaga ccaacaaggc ataattgtga aaatccagcg agacagaaag
     2761 gaaccattta acacaatatc aataaaacca gatcaagact cagatgagct caaaaagcta
     2821 atacttgatg catacaatac gattcgctag tacaagactt cgaattttgc aaattcctct
     2881 ttgaattttt catttcttat ttcaatgtct tcgacgcgtg cgtttgcagg tcctgcatga
     2941 caccactcaa ccatattgct cacaccggca tcttcgcctt ctatgattgc ctcaaccctg
     3001 ccatccttta gatttttaac ccagcccgat acacggttct tttttgctgc gaccttcatt
     3061 gcctggcgaa aaaaaactcc ctgtacctta ccctttatga aaagatggac gcgctgcttt
     3121 accatttgaa aagagtctcg tcttgggatt aatcaatttt aggatttatg aaatttttat
     3181 agaattacac tctttctttt agtctggctc gcccagtctt tcttgcagct atgcttccaa
     3241 ctttggcacc aggtggcgcg tttcgtgaaa ccgttccgct ctgacccatg tgttggtgtc
     3301 ttcctcctcc gtgtgggtga gcatatgctg cttgtgcaac tcctctgact attggatatt
     3361 tgtggccctt tgattggtat ctgcgccatt tgtttcctgc attaagaaaa ggcctatctg
     3421 atactccacc gcctgccaaa attccaacca tcgctctgtt ttttggattc agcgtagtaa
     3481 aacttccaga tggaagcttt attgtaactc catccacgcc gtgtgaaaat actgtcgcag
     3541 aattcccagc agtttttgca attgcaccgc catccccaaa gtgtttttcg atattgcaga
     3601 ctatggttcc atctgggatg ttttggatac tgataacatt tccatttgtg atttctgcgt
     3661 taagaccaaa ctttactgtt gagccaattt tagttcctaa aatcgccggc accaaagaaa
     3721 ctgagccgtc ctcaaatctg acctttgcaa gtggtgcatc tctgcctctt tcatgaatca
     3781 gatctatgac tgtgcctgtg tgatcttcgg acagatcaaa atgtgggtac ttggctggtg
     3841 ctaacttgcc agtcgaagct gctctaaatt gcatgcctcc tcggccacgc cttctaacta
     3901 gtggtcgttt acccattttc gatgatggct tggctttaag attttaagct ttgtattgta
     3961 tgaacaaaat ctgacaaaga tataaaaaat acgctgagat gttaattcta tgagccaaag
     4021 cgcactctcc cttaaagtac ttgaggcata tacgagagac gttggccgcg gtgtggctcg
     4081 aattgattac gattctatgg atacattaaa tgcatcaact ggagatgtaa tagaaatcaa
     4141 aggcaagcgc aggaccgttg caaagtgtct cccattatac ccatccgatg agggcaaagg
     4201 aattatcagg attgacggac tgggaagaaa caacgcaggt attgcaatag gagatacaat
     4261 ttctgttagg aagatcaagg cagttgcagc tgaaaaagta gttgttgccc cacttgaggc
     4321 aatcccccca atcgatgagc gttatttggc ggacgcccta gaaagcgttc ctttgatcaa
     4381 aggcgataac gtcatggtgc cttattttgg cggacgattg acatttcagg taattggtgt
     4441 aacgcctgca gctgatgcag tcctagttac gcaaaaaact gtatttcaca tagcggaaaa
     4501 gggcgaaacg ttacgaggcg ttcctcaagt aacatatgaa gacattggtg gcctcactga
     4561 cgaaatcaaa aaggtaagag agatgatcga gcttccacta aggcatccag aaatctttga
     4621 aaagttggga gtagaggcac caaagggggt actcttgtat ggcccacctg gcactggaaa
     4681 gacactacta gcaaaggcag tggcaaacga aagcaacgct cactttatca gcatatcagg
     4741 accggaaatt atgagcaagt tttatggcga aagcgaggca aggctacgtg agatctttaa
     4801 agaggcaaga gagaaatcac catcaataat tttcgtagat gaaatagatt ccattgcacc
     4861 aaaaagagaa gaggtgacag gagaagtaga gcgaagagta gtttcgcaaa tgctttcact
     4921 catggacggc ctagaggcaa gaggcaaggt aattgtcatt gcggcaacaa acaggccaaa
     4981 cgcaattgat cctgcactaa gaaggccagg acgatttgac agagaaattg agatcaaggt
     5041 gccagacaaa aagggcagaa aagacatact caacattcac acaagaaaca tgccgcttgt
     5101 tactgatgaa aacgattcca actatgttaa cgtggataaa atttccgcag tgagccacgg
     5161 ctatgttggt gctgacctag aatacttgtg caaagaagcg gcaatgaagt gcctgagaag
     5221 acttttgccg gaattgaccc ttgaggatga aaaggttcca cctgaaacac ttgataagct
     5281 catagtaaac aatgatgatt tccaaaaagc cctaattgag ataacgcctt ctggaatgcg
     5341 cgaagtattc attgaaaatc ccgacgtaaa atgggacgac attggtggac taaaggaagt
     5401 aaagcagcaa ctacaagaag cggtagaatg gcccatgaag tatccaggac tatatgacaa
     5461 actagggcac aaaatgcctc gcggaatttt gttgcatggt cctagcggtg ttggtaagac
     5521 attactagca aaggcagtcg caactgaaag tgaggcaaac tttgtatcag taagagggcc
     5581 tgagctccta tcaaagtggg taggagaatc tgaaagagga atcagagaaa ttttcagacg
     5641 ggcaagacag gcatcgccat gtgtaatatt ttttgatgag gttgactcta ttgcgccaat
     5701 taggggtgca ggttcggaaa cggcagtaac tgagcgtgta gtaagccagc tcctaactga
     5761 actcgatgga atggaaaaca tgcatggagt aatagttctt gcagccacaa acagagcaga
     5821 catgatcgac ccggcactcc tcagacctgg cagatttgat aaaattgttc agattccaat
     5881 gccagacaag gacagccgaa gacaaatctt gcagataaat gcaaaggaca taccaatagt
     5941 tagcgacaaa aatgatcctg gctatgtcaa cttggataaa attgccgaaa ttacagatgg
     6001 aatgagtgga gcagacgtag ctgcaatcgg aaacactgca gtttccttag tcatacacga
     6061 atacttagac aagcatccaa ccaaagaaga attggataat gcggcaagca acgccaaggt
     6121 tacgatgagg cactttgaag aagcagtcaa gaaggtaaag acgcaaaaag acctaaagat
     6181 aggccaaaag atagcagtgc cctactacag atagtattaa aataataaca aaactagctc
     6241 cttcataaat gtcagaatat cgtggttact ctggaagggc actcgaattt ctaaagcaaa
     6301 acaaggtcaa agtcggcgac acaatcaaga taacaaccga ctctgattac actggaacaa
     6361 ttatgccgcg atacgagcac acagacgaca agcatattgt tctgaaattc aaaaacggat
     6421 acaatgtagg gctagaaata actcaggtaa aaaaagtcga atacatctca actattgagc
     6481 ccaatcaaga aaaaaatact acgactgtgt ttaatccttc gttgcccaaa gttttgcttc
     6541 tctccacagg cggaacgatt gcaagcaggg ttgactaccg aactggcagt gttactcccg
     6601 ccttgtccgc acaagacctt aacgcatctg tgccagagct ttcagagatt gcaaacattg
     6661 atgccgaagt gttattttca gaatattcag aaaacctgac tcccgaacac tggataaaga
     6721 ttgctcaaag gcttgactct ttggcaaaat ccgattacaa aggaatcatt gtggcgcatg
     6781 gaacagacac catgcagtat acagcagcct ttctttcgtt ttcgctggca ggtttcccaa
     6841 agccaatagt tctagtcggc tcacagcgat catcggacag accatcgtca gatgctgcaa
     6901 caaatttgat ttctgcaaca agattcattg taaattgtaa agtaaatgga gtttttgttg
     6961 ccatgcacaa taccgaaagt gatgatgaga ttgcttgcca tcttggaacc cgtgttagaa
     7021 aaaaccacac aagcaaaaga aacgcctttg aaaccatggg tggaaaaccc gcattcatca
     7081 tcacacaaaa tgagataatt cagaactttt caggctcgta tttcaaagaa gcacaataca
     7141 cgcccaacat aaaaattgac acaaaagttg cgctcatcaa gtatcatcca ggctatgacc
     7201 caaagctagt agatgagatg attaatcttg gatacagggc cataattttt gaaggaaccg
     7261 gtcttggcca cattggaaaa accatgtacg atgccatcaa aaaagcaaga gaaaagaaga
     7321 ttttccttgg catgacctca cagtgccttg atggccgagt caacatggca gtttatgaga
     7381 gtggaagaga cctgctacac cttggcatag tgccgttatc tgatattatt tcagagactg
     7441 cactggtaaa ggcaatgtgg gctgttgcca cacacgacaa aaatatcgaa gagataatgc
     7501 tcaaaaacat tgcatctgag atcacagaat aaaatttaaa gtgacttccg caaaggacga
     7561 gtgtgtcaga gatacagcca gacaaaattg gattaaaggt tggcctagaa atacatcaac
     7621 agttagcaac aaacaagaag ctgttttgtg gctgcaggcc agtcgaatca gcagagtttc
     7681 caatcaggtt tgcaagaaaa ctgagattga caaaaagcga gcttggaaaa tacgatcctg
     7741 ccgccatgtt cgaggcttca aagtcaaaga tgatttacta taatgcaaat ccagagagca
     7801 gctgtcttgt tgaatacgac gaggagcctc cacatagtct tgattctgat gcaaaactat
     7861 cagctttaat ttttgcgtct gcattgcact ccaatgtttt caatgagggc tacgttatga
     7921 gaaaaattgt cattgatggc tccaacacgt ctggctttca gcgaaccatg cttgtctcgc
     7981 agggaggcta catagatgtt gagggcaaca agatcggagt gcaaacgata tgcctagaag
     8041 aagatgccgc aaagcttctc aaagacaacg aaagtacacg agaatacgcc ttagatcgac
     8101 taggaatacc acttgtagag atagccctag agccagttag cggctcacct gcacaagtaa
     8161 aaaaaatcgc acttaccttg ggaagactgc ttcgagtcac ccatcgtgtt gcaaggggcc
     8221 taggctcaat tcgacaggat gtcaacgttt cagttagcgg cggcggtatt gttgaggtaa
     8281 aaggagtcca gcagctagac cagttagaaa aagtgattcg ttatgaggca aagcggcagc
     8341 atggcttgca gttaattgct gaaaaattgc aaaaaatcaa tcttgattca atatcaaaag
     8401 atgacgtttt agatgtgacg ccgcaatttg caagctgcaa atcaaaaata atccaaaaat
     8461 cactcaagga tggcgcagca atcaaagcat taagaatcaa aaactttgca ggaatgtttg
     8521 gctacgagcc atacgaggga ataaggcttg gaagagagct tggacagcaa gttagattct
     8581 ttggcattgg gggggttttc cactctgatg agctcccaaa ttatggaatc gagcaaagtg
     8641 atgttgatca gctcagagac attctaaagc tggaaccaaa cgacgggttt ttgatcattt
     8701 caggatcaaa agaaaaaatt gattttgccg ttgatgctac cataaacaga ataaagaatg
     8761 caaagaatgg ggttcctgca gagactcgtg cagccacacc aacaggagaa actgtctttt
     8821 tgaggcctag gcctggctca tctagaatgt atcctgagac agacatacca ccagtaataa
     8881 taactgatga ggagattgca tttgcaaaaa acaacattcc aagatcatgg gatgagacaa
     8941 ttttagagct tcagaaaaaa tatggtatta acgcgcagct agccgagcaa atctttgact
     9001 ctgattattt ggatttgttc gagtcgattt gtgcctataa aaagaataat ccgaactttg
     9061 ttgcatctat actatgttca acaataacga atctacaaag gcaaggacta gattcgtcat
     9121 tgctcaaagc ggagcacatt ttgaggtcct ttgagctttt agcacaagac aaaataacaa
     9181 aagaatcact tgagatgatt tttgagagca taatgtcatc taaaataagg tcgccagacg
     9241 agtttgttca aaaacattca ggttcatttg atgaaaacga gttagagcag atcctcgatg
     9301 agctgataaa aaagaattta gagatgatcc aaaaacaagg tcttcactcg acaggatttt
     9361 tgatgggact tgcaatgaag actcttcgtg gcaaggtttc aggtgaaaag ataagcaagt
     9421 tgctggaatc aaagattaca aaaataatag aaaaataaaa actcgtagaa aactagtcta
     9481 gtctaatttt cttcttcctg ttcctctgcc tgatgatacg tatcgacctt ttgctgaggg
     9541 tctggtggtt tctggggtgt cacctagttt tcttttgcca gtgccgcgtc cggaaacaac
     9601 atattcgtct tttgcttttt ccttcatttt cttctggtat tctttgaatt tttcgccggc
     9661 ccaagtctca tcttcagatt cggctactct tcgtgaggta cctcttccac ccactttaac
     9721 aatttttccc attattgata ctagaactca ctatggttta aagttgttcc tgaaatttta
     9781 atttgataaa atgcgggagg tgggatttga acccacgaac tcctaggaga taaggatctg
     9841 aaccttacgc cgttgaccgg gcttggcgac tcccgcaatc aagggttttt agtgccaaaa
     9901 taagttcttt attgaatctg caaagagaga aatatcattc attttctact gtcgtattgg
     9961 agtttaagga aatttattgt aacaattgca agcagacatt gggtcaatac aatgtcaaat
    10021 actttacaga cgccaaaata gcagaactga taaaatcaaa tcacgccttg cattatagaa
    10081 atggccacca gttatccatt cggaaaatca cgaaatagtc tattccaatg cttgtccaaa
    10141 cttttgtagc aattcttttt ggtgtttgct aagcttgctt gggacttcca ctacaaatct
    10201 tacgtattgg tctccgcgtc ctcgtgagcc agaataaggc aacccctttc cttttagttt
    10261 tattatggtg ttaggctgag ttccctcttc tactttgacc ttttcacttt tttctagtgt
    10321 aggaacctcg attgtttttc caagacaggc atcaacaatt gagatttttg catcgtaaaa
    10381 tatgtcggct ccatcccttt tgaaaaattg atgcggcttg actcgtattc gcacaattag
    10441 atctccgttt atccctccac gtattggttc tccttctccg ctaatcacat aatcgccatt
    10501 atcaattcca ggcgggatat caaatgacag tggttttgtg ccctttgtct ttcctgtgcc
    10561 tttgcaatta gaacagggtt ttggtatgaa ttttccctct ccacgacatt tggtgcatgg
    10621 ctgcactgtg acaaatgttg agaatcccat gttgcgtgtt attcttatct gaccttgtcc
    10681 cctacagtta ggacaagttt cggagctagt acccggtgca caaccgcttc ctttacaatt
    10741 attgcaatca atgttttttt tgacatcaat ttcgagtctt ttgccagaaa atacatcctc
    10801 aagtgttatg ctcgtctcat agagaatgtc ttgtcccttt tgtcttgcaa agccaaaccc
    10861 accagacctc ccaaaaagcg attcaaaaat tgaatcaaag ctgccaaagc caaagttaga
    10921 aaagacatca tcaaagttac ctcttgctcc ctgaaagatg tcttcagtag agtatctccc
    10981 cccaagacct tcatgcccat actgatcata gactttacgt ttttctggat cagccaaaac
    11041 ggcatatgcc tcagaaattt ccttaaagtg ctctgcagcc tccggggact tgtttctatc
    11101 aggatggaat tttagtgcta attttcgata ttgggatttt atggtgtcta cagaatcggt
    11161 tttagatact cctaatacct cataatagtc tctttttgca gacattttcc cattttagtg
    11221 aaatcgtcgt atatgcataa attagcttgt cgttcctgtg ccctgcgttt tttgatccgc
    11281 agtcccgtct tgggtgtttt ggtctgaggg tccgctctgc gattgttgtt cttctgtgtt
    11341 tggcggtggg gctgcgttct tgtagagttc tgttgatatt tggttgacaa ttgtttttag
    11401 cgcatcaagt tttggtcgaa tttgttctga atctttgtcc agcacttctt tgagctcctt
    11461 tattgcctct gatattttga ttccttgctc ctgtgtaagc ttgtctttta ggtcctgggt
    11521 tagcagcttt tcagctgtat agacgaagct ttccgcttcg tttttgatgt ctactgtttc
    11581 ctttttcttt ttgtcctcat cggcaaactt ttctgcatct tgttttagct tttcaatttc
    11641 ttcttttgat agcttggagc ctgcttggat tgttattttt gcctcctttt tggtgcctag
    11701 gtcctttgcg gtgacattca gaattccgtt tgcatcaatg tcaaatttga cttcgatttg
    11761 tgggattcct cttggtgctg gaggaagtcc agtgagatta aagcttccaa gggaaacgtt
    11821 gtctgctgcc atgggtcttt cgccctgtac tacgtgaatt gtaacggcgg tttgattatc
    11881 tgctgctgtt gtaaagattt ttgtctttga ggttggaatt gtagtgtttc tctcaattag
    11941 cggctctcgc aagccaccaa gtgtctcaac tcccaaagta agtggagtta catctaagag
    12001 tacgatgtcg ctggtcacat cacctgcaat tattcctgcc tgaattgcgg cacctactgc
    12061 aactgcctcc attggatcca caccagactc tgggcttttg cctattacgt cgccgacaaa
    12121 ttttttgact agaggaattc ttgttggtcc gccaactagg actatttttg agacgtctga
    12181 tggagcaagt tttgcatcgc gaagagcatt gtcaactgag ccgcgacatt tttctactat
    12241 tggcctgatg agctcctcaa attttgccct cgttagcttg atttccagat tttttggtcc
    12301 agcagattgg tcatacgata ggaatggaag attaatgtca gtttccatta ccgttgaaag
    12361 ttctatcttt gccttttcgg aagcctccct gattcttgtc attgcagtgc tgtcctttgt
    12421 aagatctacg cccgtagtct tgcggaattc gttgacaaca taatcaatga gaatcttatc
    12481 catatcggtg cccccaagct gtgtatctcc ggatgtgctc aaaacttcaa aaacgccacc
    12541 tcccatttcc ataatagtaa catcaagtgt tcccccgcca aaatcaaaga ccaaaatttt
    12601 catgtcttgg cccaccttgt ctagtccaaa tgcaagcgag gcagcagttg gctcgttaat
    12661 tattcttaca acatcaaggc cggcaattgt tcctgcgtcc tttgttgcct gcctttggtt
    12721 atcatcaaaa taggcaggaa ccgttatgac tgctttatcg attttttcgc ctaggaatgc
    12781 ttcggcgtct tttttgattt tttgtagtat aaaagcggaa atttgttgag gtttgtagtc
    12841 tttgccttga attttgaaaa catagtcact tcccatcttt ctttttgctg caacaatcgt
    12901 attgtctgga ttggtaattg cctgtcttct tgcaggctcg ccaactatga gctgaccatc
    12961 ttttgtaaag gcaaccacag aaggaaatgc cttaccgcca atagatgtcc cttcagcagc
    13021 aggaataagg acaggttttc cacccatcac tactgctgca gcagagttac tcgttcccaa
    13081 atcaattcca attatttttg ccattttttt cactttagat tattttgttg tggtttttta
    13141 gatatttcaa ctagtgtcgg tctaagcgtc cttttatgag aaatatatcc tttcctgatt
    13201 tcctttgtaa ttgtgttctc atccagattt tcgtcctcca ttaccgatat tgcttcatgc
    13261 aggttgggat caaatatttc tcctagtgcg ttaattgggg caattccata ttctgagaga
    13321 agtgcttcaa tgtttttcaa aataaagtca aggccccgtg cgtttggatt ttcttttttg
    13381 agaatttcct ttgctctgac cagatcatcg taaattgtta gaaacgttat catgaatttg
    13441 tctagttttt gattaacgcc gttttctata tcacttttag tttttctttc caagtttaca
    13501 aaatcagcaa gtgagcgttt gagcttatcc tcacacgata caagactttg ttgtaattcg
    13561 attattttgt tatctagttc ttttatcagc tgatcagctg attttggttc agcgactatt
    13621 tccagatcct cttctgttgg tagtgattgt gaattttcta atttgttttc gtcgttgttg
    13681 ttttctgaca agacaagtaa ttttttttct attctagttt taatctttgt gcaagatttc
    13741 gcagagtggg tttcccttta ttataatgag gtcgttgtca tactttttgt gaaggcgctc
    13801 ataatcagac tttgagcata tgaccaaaat tgtagtcttg tcgttgtgga acagatccag
    13861 atttggatcc gcaaatgcct caacatcacc gatatagtcc cgaagcttag tgattagatt
    13921 ttgtagcatc tcgattttgt ctcctcgcat ctcgtgttga tccaaagacc atgatagtgc
    13981 aattttcgta cgacttgcct tcaagtcatt ttttttcaga gttgtcctga tttcatcaat
    14041 tagtcttttg atgtagccat ctattccttt gagacttccg tttattagat acatcagggt
    14101 gttgactccc tcaaacgacg tacccagtga gcgcaagtcg tacaagccat ttaccatatc
    14161 atctaagaat atgttttgaa agtcatctga ttggagatct gtctttgtat tatcatcctg
    14221 tgcaaacttg catacctcca gaacgctaca caatgatgaa aatctggaaa gtacatttat
    14281 tgcatgaaaa tgctcagctg aagaaatgcc aacaaacgag atttcttttt tattcccagt
    14341 tagcaggttt gcaagacttt gatcctgctt gctgttgtat gagccaatta ctttgggtgg
    14401 ttgattcccg ccccccagtg gataataata gtaaaaaatt gatttgccta cttcaagtcc
    14461 tgtcacatgt tctaaaagcg atatgttttc gccgtttcct ccaattcctg taggaagtat
    14521 atagatcaca gagctcccct ttttgaggga ggacgtagca tccttgaatt ttgaatgaac
    14581 ctcggttttt gtttcctgac caatttttct aattcttggt gcaaaaaaca ggtactgggc
    14641 ctttgatatt gccacatcaa ttggctccat tgccaacaat ggctcatctt cttttagtga
    14701 ggaaacgtta ggataggtct ttgcaatttc tgcctttagt gatatggctg actgcgagga
    14761 ttcgtcgata atgaacacat ttgcaccgcg tattgccatc tggcatgcaa tagcgtatcc
    14821 ctctgtacta agaccataaa caaccactct atccgctacc aattcattgg tgctaggtaa
    14881 aaggactaag aaaaacttat tgcatcagtt atcacatagc acctatcttg aagatttggt
    14941 ttgacatttt gacaccaaaa cagatcttat tttttgagcc aatgataaaa cagctgcaaa
    15001 agagcaacca aattctttgc acctcaagaa attatcgaga ggtaacacac cttgcaaagc
    15061 tcagaaaaat gaagattgca ttaattggaa agcatggtgg tcagagcaaa attgacaagc
    15121 taaatgccag tttgagtaga atgactgcac tggccagaca g
//
LOCUS       DASWKH010000049        15149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3084073, whole genome shotgun sequence.
ACCESSION   DASWKH010000049 DASWKH010000000
VERSION     DASWKH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084073"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGA92_03635"
     CDS             <1..744
                     /locus_tag="VGA92_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX9845537.1"
                     /translation="MLRRKKSDVLTELKEKVRYKEIIDSDINYYNMELEKIWTKLEAE
                     RKVAQTAFDASTAYQRAIQSERQAAGVAKLPHVINFVKNIMEIEESVVVFCHHKAIHS
                     LLHQSLAEFKPASIIGGQSDTYRQQQIDSFQNGETKLMIAGLRAGNVGINLTRAKYVI
                     FAELDWSPAIHLQAEDRLHRIGQKNTVFAYYLIGNGTLDEHVAQVLVDKSYEIDSIMD
                     NKAESFENKEKAQLILAQIQDKVKSILKN"
     gene            complement(741..2036)
                     /locus_tag="VGA92_03640"
     CDS             complement(741..2036)
                     /locus_tag="VGA92_03640"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9845538.1"
                     /translation="MSVKILLIFVSALLVLNTNGVFAQEFPSYNLKVETIAENLEVPW
                     AIAFSPDGRIFFTERDGNVRIIEDGITLQEPALTLNVGRIEGGLLGLALDPEFEKNHY
                     LYLYYTYSELLSTYNRVSRFTENDNKLSDEFVLIDKIPGAAVHDGGRIRFGPDNKLYI
                     TTGDAANAPLAQDLRSLAGKVLRINSDGTIPADNPFSGSAVYSLGHRNPQGLDWEPVT
                     GKLVVAEHGPSGERGFAHDEINIVEAGKNYGWPNIVGSQTDPRYISPIFHTGEIAWAP
                     SGASFYNSDKIPELKGKYLVANLRGTHLHAFEIDTEQNEIISDESLFSGQFGRLRDIS
                     VGNDGYLYMLTSNRDGRGSPTPNDDRILRIMPLLETTSYPPPLKQLKNGVSPYDIKCS
                     DDYVLVLKQMNNSPACVKPATAAKMIQRGWFSTDPTQVV"
     gene            2098..2349
                     /locus_tag="VGA92_03645"
     CDS             2098..2349
                     /locus_tag="VGA92_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845539.1"
                     /translation="MDFLLEEEMIDLLTFCLQNPSSADMTQKKTRITEIGKELFADGG
                     VDALENMFFVLENRIKEEIGQDPRPFRSLWNGNSSEWNY"
     gene            complement(2371..2514)
                     /locus_tag="VGA92_03650"
     CDS             complement(2371..2514)
                     /locus_tag="VGA92_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX9845540.1"
                     /translation="MMIGYCVKCRAKREMAGTKAVTLKNGKPATKGTCPTCSTAIFRI
                     GKA"
     gene            complement(2633..3913)
                     /locus_tag="VGA92_03655"
     CDS             complement(2633..3913)
                     /locus_tag="VGA92_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9845541.1"
                     /translation="MSLTTAQKKIVAGSFLGWSLDGYDIVLMLLVIPSISQLFFPSEN
                     PIFSIIATFASYTITLIMRPLGSAIFGIYGDKFGRKRAMIITILGFSLATFSVGLLPT
                     YATVGIMAPVLLIMIRLIQGIFAGGEWGSGAVITIESVPKQRRGTISGFLQSGFSFGF
                     LLAAIAFQIITTAFPGEAFEEWGWRILFFTGIIPGFVALFIRLSMDESPLWIEKSKED
                     RLERSPLRKIVFAKEHRREFFLCACIMTGLVYMYHGSISILPTYLQQFGQIQRGEVAL
                     VMIYATTSSWLGMIMTGWLSQKIGRKNSILCFVAASVLVSIPLATMILANGMLVVFFV
                     IVYAFVVSTASGPIPAFFSERFPTQIRNSAAGFSYNAGLIFGSWSPLIALSLMSSVGK
                     DLVSVALGINIIIGAIIVIIPTILSRETKDVELD"
     gene            complement(3963..4388)
                     /locus_tag="VGA92_03660"
     CDS             complement(3963..4388)
                     /locus_tag="VGA92_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845542.1"
                     /translation="MPKTVAFFVIFLLILLPFQNVFALEGTERIELRNPRLVNAFGES
                     VSQNINTHQQVRVQTDVTNKQNIPQNFAYIVQILNEDKVTLKLTWFAATLNPHQTLSL
                     AISWVTDNPGTYTAEIYVWDSIKDAYALTPPIELKIIVS"
     gene            complement(4468..5793)
                     /locus_tag="VGA92_03665"
     CDS             complement(4468..5793)
                     /locus_tag="VGA92_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9845543.1"
                     /translation="MQAFATPQTLRGEIINLASSCGLEKPCFTHMLDYTIELFESQGL
                     GKDYYGYHNITHELEVTYVSLIVLKWKSILNNIREEDFKYLYAAALFHDFDPQKSVDK
                     PHEENVIKFLERDNKVKQLCKDAELDLDIIKALIHRTTYPWKGNLKEEAISHIKELFE
                     RSQITKNNMQAQDYYMKLGWLLSVIDRVSGYSLGDFVKAMDMAKKNAHALAWHPSLIV
                     KRSVAYFEDLLNDESEMCDTVLRALPRHMRKNFMDVVTGFLNLRQQEIKIQSDYLYEN
                     LQLIPKIESMRIRNDSDFVKTLLDIYGELPTPLQIDREHFENTVKEPKTILNTLRLGN
                     SDGPIIGFAKGGPLENYQLRQEISDENYGKNNTVFLEPIALKMGYWGLQGGSEMRHLF
                     TMQAHSMNYTFLTSFALRDVIQRRVENNEKAEFVVKFDPERWDYYRIEL"
     gene            6004..6213
                     /locus_tag="VGA92_03670"
     CDS             6004..6213
                     /locus_tag="VGA92_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845544.1"
                     /translation="MSTQKQNKTKIKSEITKLLDDGCSDKTIIYKKIQSDFGISQNEA
                     RAACKEIKIELLTKLKTLQSGMLEL"
     gene            6255..6656
                     /locus_tag="VGA92_03675"
     CDS             6255..6656
                     /locus_tag="VGA92_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845545.1"
                     /translation="MGIDGNILFKLAECQNQVPCMMKIGDASYKVDSIELTYSEVPVT
                     KPTTRGGVYFSEKMAFKVKAKVSDLAVANVLSKTMLGPNQDFEKIRLLTNVIVDTNKK
                     EMVIFANLTNYVQKSSGLELNLVVIGTELSD"
     gene            complement(6645..7268)
                     /locus_tag="VGA92_03680"
     CDS             complement(6645..7268)
                     /locus_tag="VGA92_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9845546.1"
                     /translation="MTKAIIVLFLIVYSLVLPTDGFFIHSGLLDAAAQQSQVIIEDRS
                     SQEIFEYYNPNEVHLAMAIIAAALVGVFLYLARDIILRKKTEYEKKEFASKQNRDYEK
                     YHSEWTTDSDDIFEEKKESAEAREFRKMLQDSKFPNYYVVLGVPPNASQNEIKTKYRQ
                     LVKEYHPDKTRDEKTAAVLAEITKAYDVLSDEEKRKTYDKYYNASIG"
     gene            complement(7320..7763)
                     /locus_tag="VGA92_03685"
     CDS             complement(7320..7763)
                     /locus_tag="VGA92_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9845547.1"
                     /translation="MVLDKTDVKILKNLLVDARLSSRQMALKLGMSTVTILTRIKKME
                     QEKIVKGYTAIIDHEKLGYDLTAIIEIYTKKGKMVEIEQEISSLENVCAVYDVTGESD
                     TVLVAKFKNRDDLSKFVKMISSKPNVDKTVTNIVLNTVKEDFRLV"
     gene            7876..8115
                     /locus_tag="VGA92_03690"
     CDS             7876..8115
                     /locus_tag="VGA92_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845548.1"
                     /translation="MIEKNFDFTKLYGRVVKYYLDKKHYTKEKANFIAQKVVQREKKM
                     RTCQNSRCGHLLYDHMRNYESCLIDKCTCREFVKV"
     gene            complement(8116..8499)
                     /locus_tag="VGA92_03695"
     CDS             complement(8116..8499)
                     /locus_tag="VGA92_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845549.1"
                     /translation="MNILIAEDNQFTATQYDRILRRYGHSITITKDGSECLQKYQEKL
                     KRKEFESLDVNPFDVVVLDQSMPKKSGSEVANEILSVKPTQKIIFASAYALTGSNETK
                     SLEDKVEYLQKPFSLNSFVQKIQGQ"
     gene            8607..8831
                     /locus_tag="VGA92_03700"
     CDS             8607..8831
                     /locus_tag="VGA92_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9845550.1"
                     /translation="MSVDELIKKGKTLLVEGKFEDALSYFEQALLLDQNNADIWNQKG
                     VTLRSMGRYDDALECFNKALQLDPADKYAS"
     gene            8893..9393
                     /locus_tag="VGA92_03705"
     CDS             8893..9393
                     /locus_tag="VGA92_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9845551.1"
                     /translation="MNNVEKILNDASLLCEDGKFRKAILLYSEALRKQPDNLDVLIDK
                     GVALQNLGRLKQALRCYQKAVSFAPQNVTALLNKGSVLHSLGRFQEAIACYDQAIFVD
                     KKCAMAFAYKGLSLGEQGLVKEALKHFKKALTIDSSYDMAKISKGIAQKILDEKNENY
                     KPVSKN"
     gene            9669..10766
                     /locus_tag="VGA92_03710"
     CDS             9669..10766
                     /locus_tag="VGA92_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9845552.1"
                     /translation="MLRELVSESLAIEKALAGDELSHKDGLELINHDNLFMVGAAADL
                     VRKKLVGDKVTFAASYYMNYTNVCAASCQMCAFYRKGDENDAYTLTAEQIEQRVAVAK
                     NMGATEVHIVGGFHPNLPLDYYENMMKIIKKNHPDLTIKAFTAAEIFYLSKLTKNSVK
                     EILLRLKEAGLDTMPGGGAELFHPEIRNQIVRGKCSGQEWLDTIEQAHSLGIKSNATM
                     LYGHIEKPEHIIDHLIKIRQLQKKTGGFITFIPLKFSLDNTELEQQNLVKHECSSIYD
                     LKIIATSRLMLAGALDNVSVYWVAYGKKLAQVALSNGGSDLVGTAFSEEIYRAAGKPT
                     NSSVLELAEMVKEIGRQPIQRDTYFRTIRTF"
     gene            complement(10763..11062)
                     /locus_tag="VGA92_03715"
     CDS             complement(10763..11062)
                     /locus_tag="VGA92_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX9845553.1"
                     /translation="MVHAEISVYPIGTDSTSVSFYVAKAIDAIKDLKIKHQITPMGTI
                     LEAESAEQIFEASKNIIEVIHRLGVKRVEIILKIDSRNDKKQTMKEKIQSLEKHR"
     gene            complement(11094..11372)
                     /locus_tag="VGA92_03720"
     CDS             complement(11094..11372)
                     /locus_tag="VGA92_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845554.1"
                     /translation="MVLVDKRILGGGLAMIIVGLSLLAYLNSISPLGTPEMTEEQEID
                     LMLKQQENRDYTNLAGLLAGVGFLLVLISFGARKRKGGAKMIEKKPPA"
     gene            complement(11631..12461)
                     /locus_tag="VGA92_03725"
     CDS             complement(11631..12461)
                     /locus_tag="VGA92_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX9845555.1"
                     /translation="MKISVGHTPDADDAFMLYGMLTGKVPSVEFTINHVVEDIEALNK
                     KALKYELDVTAVSVHACAHLPNYTILRSGGSFGIGYGPIVIAKENITHEKLRTLKIAI
                     PGKMTSAFLLLQLMIGKFDYFEMNFSDIPEAVREGKVDAGLVIHETQLSYEQESLQKI
                     LDVGQWWDKTTNGLPVPLGTNVMSNKFDNTTIQKFDDYLQASIRYGLEHQDEALQYAM
                     KYSRGKPADLIKKFVKMYVNDITVEMGQSGEAAIRKMFELAVKQNLIEPFDIKISQRS
                     "
     gene            complement(12604..12891)
                     /locus_tag="VGA92_03730"
     CDS             complement(12604..12891)
                     /locus_tag="VGA92_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014126156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX9845556.1"
                     /translation="MSFNDNSDRPSRRFGGGGYGGGGRRRFNDRQFDDTPKPVETGKE
                     YDVSITETSRQGDGIARVEGFVIFVKGGQPGQEAKIKVTQVGRRFATAELV"
     gene            complement(13050..13874)
                     /locus_tag="VGA92_03735"
     CDS             complement(13050..13874)
                     /locus_tag="VGA92_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845557.1"
                     /translation="MLKQQRQYGSFLVTAEDLIHPPIFINKTSTLYDAAEKILMHNIS
                     GIIVNSREKYDIISQKDIAGALISEKQNISSIPVSQKMQELVMVDQYAPISNCAALML
                     SKRTNALGVKDHSGVKGIMTKHDLVKFFQQNIVDETKLADIMSVGSFFVPQTTTLYNA
                     LTKMLDNQISRLLVKDENGKSIGIVTYKSFLRHTIYNSNRYDDTVFATGFGKTCTVGE
                     IMRKQLISISIQTSLAKLAKILIDYRIHGVAVTQNQKIVGFVTEKDIVRQLAKIQV"
     gene            13986..14894
                     /locus_tag="VGA92_03740"
     CDS             13986..14894
                     /locus_tag="VGA92_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX9845558.1"
                     /translation="MNTIEAYEKDISLQVGFLQKIKLQKPLSQQRLDKTVFCGTGDSL
                     ASAMLAESFSNFRVKALDPLDIIKNKKLVQGKFAYFVSVSGNTISNIRAAKLANNSTA
                     ITKNRISPLAKSCNQPIQLRYQDSHVLTSGSIGFLASMLMCVSLVFDFRIKSAKKLFM
                     EAQSQACKILLKNKIYVIGNQHTYPLAMYASAKLYEVLGSDAHYERIEQFSHASLFSA
                     KKGDTVIIFEKTNRHNKQLVAQLKKLGLSVYNPSIMTADKISQVIFYTFVSQLMTLYD
                     AKRKQLDDCHFIIEKKFRNASSTMIY"
     gene            14963..>15149
                     /locus_tag="VGA92_03745"
     CDS             14963..>15149
                     /locus_tag="VGA92_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9845559.1"
                     /translation="MKYKATEQILKIIKNKENIRNFGVIAHVDHGKTTMSDSLLGYAG
                     IISPQAAGRALALDSMKL"
BASE COUNT         4454 a         3009 c         3038 g         4648 t
ORIGIN      
        1 atgcttaggc gcaaaaaatc agacgtccta actgagctaa aagaaaaggt cagatacaag
       61 gagataatcg actctgacat taattattac aatatggagc tggaaaaaat ctggacaaaa
      121 ctagaggcag aaagaaaggt ggcacaaacg gcatttgacg catcaactgc ctaccaaagg
      181 gcaatacaaa gcgaaaggca ggcagcagga gtggccaagc tgccacatgt gatcaatttt
      241 gtgaaaaaca taatggaaat tgaggaaagt gtagttgtgt tctgtcacca caaggcaatt
      301 cactcgcttt tgcaccaaag ccttgctgag ttcaagcctg catcaataat tggaggccag
      361 tctgatacgt atagacaaca gcaaatagac tcgttccaaa acggagaaac caagctaatg
      421 attgcagggc ttagggcggg aaacgttgga atcaacctga ctcgagcaaa atatgtgata
      481 tttgccgagc ttgactggag tccagcaata cacctgcagg cagaagaccg cctccacaga
      541 attggacaaa aaaacacagt ctttgcatat tatttgattg gaaatggaac gcttgatgag
      601 catgtagcgc aggtactagt cgataagagc tatgaaattg attctattat ggataacaaa
      661 gcagagtcgt ttgaaaacaa ggaaaaggcg caactcatct tggcgcaaat tcaagacaag
      721 gtaaaatcta ttctaaaaaa ctaaactact tgcgttggat ctgtactaaa ccatcctctc
      781 tgaatcatct ttgctgcagt tgctggcttt acacatgcag gtgagttgtt catttgtttt
      841 agaaccagca cgtaatcatc agaacacttg atgtcatatg ggctaactcc gttctttagc
      901 tgctttaacg gtggaggata actcgttgtt tctaaaagcg gcattattct gaggatcctg
      961 tcgtcgtttg gtgtaggaga gcctcttcca tcgcgattgc ttgtaagcat gtaaagatac
     1021 ccatcgtttc caacactgat gtcgcgcaac cttccaaact gtccagaaaa caacgactcg
     1081 tctgatatga tttcattctg ttctgtgtca atctcaaagg cgtgcaggtg tgtcccccgg
     1141 agatttgcaa caagatactt accttttagc tctggaattt tgtcagaatt gtaaaaactt
     1201 gcaccggatg gggcccacgc aatctcgcca gtgtggaaaa ttgggctgat gtatctcgga
     1261 tcggtctgac tgcctactat gttgggccaa ccatagtttt tgcctgcctc tactatgttt
     1321 atctcgtcat gtgcgaatcc tctttcacca gaaggcccgt gttctgctac aactagtttt
     1381 ccagtaactg gttcccagtc aagtccttgt gggtttctgt gtccaagcga atacacagct
     1441 gatcccgaaa atggattgtc agcaggtatg gtgccatcag aattgatcct caaaactttt
     1501 cctgcaagtg atctgagatc ttgtgcaagc ggcgcatttg ccgcatctcc tgtagttatg
     1561 tacagcttgt tgtctggtcc aaaacgtatt ctgccgccat cgtgtactgc agctcccggt
     1621 attttgtcaa tcaacacaaa ctcgtctgat aacttgttgt cattttcagt aaatcttgaa
     1681 actctgttgt atgtagacag taactctgaa tatgtgtaat agagatacag gtaatggttt
     1741 ttttcaaatt cagggtctag tgcaagtccc aaaagcccac cttctatacg tcctacattt
     1801 agtgtcagtg ctggttcctg tagtgtgatg ccatcctcga tgatgcgaac attgccatcc
     1861 ctttcagtga aaaaaattct gccatctggg gaaaatgcaa ttgcccatgg aacctccaaa
     1921 ttttctgcga ttgtttcaac tttgaggttg tagcttggga attcttgtgc aaaaacacca
     1981 tttgtattta gtacgagtaa tgcagataca aaaatcaaaa gaattttgac actcatgata
     2041 ctattttgtc tcaaatcagt aaaatactat ttattacaaa tttaatcaag ctttgctatg
     2101 gactttcttc tagaagaaga aatgattgac ttgctaacct tttgcctcca aaatccgtcc
     2161 tctgcagaca tgactcaaaa aaagacgaga ataactgaga taggaaagga gctttttgcc
     2221 gatggtggtg tagatgcgct agaaaacatg ttttttgtac tagaaaatcg catcaaagag
     2281 gagattgggc aagacccacg accgtttcgc tcattgtgga atggaaactc ctcagagtgg
     2341 aattattgat taaaaaattg tagaaaaggc ttatgccttt ccgattctaa atatagctgt
     2401 tgaacatgta ggacatgttc cttttgttgc aggctttccg ttctttaggg tgactgcttt
     2461 tgtgccagcc atctctcgtt ttgctctgca tttgacgcaa tagcctatca tgatatgaat
     2521 actagaaaga tctgatatta gcggaaggct gtaaaaatta tttaacacgc ttgtggagta
     2581 gcgatctaaa atgaagtact atgttttgtg ccagtttttc caaacaaaac ggtcaatcta
     2641 gctcaacgtc ttttgtctct ctgctaagga tggttggaat tataacaatt attgcgccaa
     2701 ttatgatgtt tatgccaaga gctactgaaa ccaaatcctt tccaacactt gacatcaaac
     2761 ttagtgcaat taggggtgac catgagccaa agatgagacc tgcgttgtat gaaaagccag
     2821 ctgcactgtt tcttatctgc gttgggaatc tctctgaaaa aaatgcagga attggtccgg
     2881 atgcggtcga aacgacaaat gcatacacaa ttacaaaaaa aactacgagc ataccgtttg
     2941 ctaaaatcat ggtggcaaga ggtattgaaa ctagaaccga tgcagctaca aaacacagta
     3001 tggaattttt tcttccaatt ttttgtgaaa gccacccagt cattatcatt ccgagccatg
     3061 aggatgttgt agcgtaaatc atgactagtg ctacttcgcc tctctgaatt tggccaaact
     3121 gctgcaaata tgtgggaagt atggaaatgg atccgtgata catgtagact aggcctgtca
     3181 ttatgcacgc acacaggaaa aactcacgcc tatgctcttt tgcaaaaaca atctttctta
     3241 gtggtgagcg ctcaagcctg tcttccttgc ttttttctat ccatagggga gactcatcca
     3301 tgctgagtcg aataaacaat gctacaaaac caggtattat gccagtaaag aacagaattc
     3361 gccaacccca ttcttcaaat gcctctccgg gaaatgccgt ggtgatgatc tgaaatgcga
     3421 tggctgctaa aagaaaacca aatgaaaaac cgctttgcaa gaatccagat attgtacccc
     3481 ttctttgttt tggcacgctt tcgatggtaa tgactgcacc actgccccac tcaccaccgg
     3541 caaatattcc ttggattagg cgaatcatta tcaaaagaac cggggccata attccaactg
     3601 tggcataggt gggaagcagt ccgacggaaa atgttgccaa agaaaaacct aaaattgtaa
     3661 tgatcattgc tcgctttctg ccaaacttgt cgccatagat tccaaaaatc gctgaaccga
     3721 gaggcctcat tatcaaagtg atggtgtatg atgcaaatgt ggcgatgatg ctgaaaatgg
     3781 gattttctga tggaaaaaac agctggctta tcgatgggat gactaaaagc ataagtacaa
     3841 tatcgtaacc atcaagtgac caaccaagaa atgatcctgc aacaattttc ttttgcgcag
     3901 tagttaagct caacgagctg atctgcgcag aagttatatt tagttttaga aatccaaagt
     3961 tcttagctta caatgatttt tagctctatg gggggtgtaa gtgcatatgc atcctttatg
     4021 ctgtcccaaa catagatctc agcagtgtat gttccggggt tgtcggtaac ccaagaaatg
     4081 gcgagggaca atgtttgatg cggattcagt gttgctgcaa accatgtaag ctttagagtg
     4141 actttgtcct catttagaat ttgaactatg taggcaaagt tctgtggtat gttctgcttg
     4201 tttgtgacat cggtttgaac ccggacctgt tggtgtgtgt ttatgttttg agatactgat
     4261 tcaccaaagg catttactag gcgtggattc cttaattcaa ttcgttctgt accctctagg
     4321 gcaaaaacgt tctgaaatgg cagcaaaatt aaaagaaaaa ttacaaaaaa tgctactgtt
     4381 ttcggcacgt ttgaatacaa aattgtgtac tactaaagag tttggattag aaaatccttg
     4441 agcaattaac caaattctat ccttgcacta aagctcgatt ctgtaataat cccagcgctc
     4501 tgggtcgaac ttgacgacga attcggcttt ttcattgttt tcaactcttc gttgaatgac
     4561 gtctcttagc gcaaagcttg tcaaaaatgt gtagttcatc gagtgtgctt gcatagtaaa
     4621 caaatgtctc atctcgcttc caccttgtaa gccccagtac cccatcttta gcgcaattgg
     4681 ttctaaaaat actgtattgt ttttaccata gttttcatct gatatttctt gacgcagttg
     4741 atagttttca agtggcccgc cttttgcaaa gccgattatc gggccatccg aatttccaag
     4801 tctaagtgtg tttagtattg tttttggctc ttttacggta ttttcaaaat gttccctgtc
     4861 aatttgaagt ggtgttggca gctcgccgta tatatcaaga agtgttttga caaaatcaga
     4921 atcatttcta attcgcatag actctatttt tggaatcaac tgcaaattct catacagata
     4981 atcggattgg attttaattt cttgttgtcg caaattcaga aacccagtta caacatccat
     5041 aaagtttttt ctcatatgtc ttggaagtgc gcgaagtaca gtgtcacaca tctcagactc
     5101 atcatttaac aaatcttcaa aatatgcaac ggaacgtttt acaataagtg aggggtgcca
     5161 ggcaagtgcg tgggcatttt tctttgccat gtccatcgcc tttacaaagt caccaagcga
     5221 ataaccgcta actctgtcaa ttactgataa aagccatcct agcttcatat agtaatcctg
     5281 tgcttgcata ttgttctttg tgatttggga tctctcaaat agttctttga tgtgtgatat
     5341 tgcttcttct tttagattgc ctttccaagg atatgtagtc ctatggatta gtgccttgat
     5401 tatatctaaa tctagttctg catctttgca taattgtttg accttgttat cccgctccaa
     5461 aaatttgatg acattttcct cgtgtggttt gtcaacgctt ttttgcggat cgaaatcatg
     5521 aaacaatgct gcagcataaa gatacttgaa atcttcttct cttatgttgt tgagaatgct
     5581 tttccatttt aggacaataa gggagacata ggttacctct aactcatgtg tgatgttgtg
     5641 ataaccgtag tagtctttac caagcccctg cgactcgaat aattctatgg tgtaatctag
     5701 catgtgagta aaacacggct tttccaaccc gcaggaagat gctaaattga ttatctcacc
     5761 tcgcaaagtc tgaggcgtag cgaaagcttg cattagtgta acgatagtct acactatttt
     5821 aaatctgtag ctgactgttc agcaattctt tgtaaaaact gcatgatatt gtgcaaatct
     5881 tacaaccaag ctcaaacagt tgggcccgat attgaaaatg tgtgtttttg atgaggacaa
     5941 acaatctgtc agggggatct gtacacacac acagtattct attgtgcctc agcaaaaatt
     6001 acattgagca cacaaaaaca aaataagaca aaaatcaaat ctgaaataac aaaactccta
     6061 gatgacggct gctcagataa aacaataatt tacaaaaaga ttcaaagtga ttttgggatc
     6121 tcacagaacg aggctagggc tgcctgcaag gagataaaaa tcgagctact cacaaaactc
     6181 aaaacactgc aatctggcat gcttgaactc taggagagta agactcatac gggaattttt
     6241 ttcccattta ttatatgggg attgacggca acattctttt caaattagca gagtgccaaa
     6301 accaagtgcc atgtatgatg aaaattggtg atgcgtcata caaggtcgat tcgattgagc
     6361 tgacatactc ggaggttccg gtgacaaagc cgactacaag aggtggcgtg tatttttctg
     6421 aaaagatggc gttcaaagta aaagccaaag tctctgatct tgctgtggca aacgttctct
     6481 caaagaccat gcttggacca aaccaggatt ttgaaaaaat ccgtctcttg acaaatgtga
     6541 tagttgacac aaacaaaaaa gagatggtaa tttttgcaaa tctgacaaat tatgtgcaga
     6601 agagttctgg ccttgagctt aatttggttg tgattggaac tgagttatcc gattgatgca
     6661 ttgtagtact tatcgtaggt ttttcttttt tcctcatccg acaagacgtc atacgccttg
     6721 gtaatctcag ctagtaccgc cgcggtcttt tcatccctgg ttttgtcagg atggtattct
     6781 tttaccagct gcctgtattt tgtcttgatc tcattttggc ttgcgtttgg tggcactcca
     6841 agtactacgt agtaatttgg gaattttgag tcttgaagca tttttctaaa ctctcttgcc
     6901 tcggcacttt cttttttttc ttcaaaaata tcgtcactgt ccgtggtcca ctctgaatgg
     6961 tacttttcat agtctcggtt ctgttttgat gcgaattctt ttttctcgta ttcggttttt
     7021 ttgcgtaaaa tgatgtcgcg agccaaatac aaaaacacgc caactagtgc agctgcaatt
     7081 attgccattg ccaagtgcac ctcatttgga ttgtagtact caaagatttc ttgggagcta
     7141 cgatcttcta ttattacttg tgactgctgg gcagccgcat caagcaagcc ggaatgaatg
     7201 aaaaaaccat cagttggcaa aaccaatgaa taaacaatca aaaacaacac aatgatggct
     7261 tttgtcatgt atgactgatc ttggctaaaa ttttattaat ttttgcatat ggcctgctct
     7321 cagactagcc taaaatcttc ttttaccgtg tttaaaacta tgtttgttac agtcttgtct
     7381 acgtttggtt ttgatgatat cattttgaca aacttgctta ggtcatccct atttttgaac
     7441 ttggcgacaa gcaccgtgtc ggactcgccg gtaacgtcat acacagcgca gacgttttcc
     7501 agacttgata tctcttgttc aatttcgacc atcttgcctt tttttgtgta tatctcaatt
     7561 atggcagtca aatcgtagcc aagcttttca tggtctatta tcgcagtata gcctttgact
     7621 attttttctt gttccatttt ttttattctg gtaaggatgg taactgttga catgccaagc
     7681 tttagtgcca tctgtcgtga tgatagtcga gcatctacta gcaaattttt taggatctta
     7741 acatctgttt tatctaagac cattgattat cattaatgct ttacaaatat taaaggttat
     7801 cagcaatttt taaaaggatt tttagctata gcctactaga cgataaattc atccattgtg
     7861 tatatatgtt gtaaaatgat tgaaaagaat ttcgatttta ccaagcttta tggaagagta
     7921 gtcaagtatt atcttgacaa aaaacactat actaaagaaa aggctaactt tattgctcaa
     7981 aaagtagtgc aaagggaaaa aaagatgagg acatgccaaa actctaggtg cggtcatctg
     8041 ctttatgacc acatgcgaaa ctacgaatcg tgtctaattg acaaatgtac gtgtcgtgag
     8101 tttgttaaag tttgattact gtccctgaat tttttgcaca aacgagttga gagaaaacgg
     8161 cttttgtaag tattcgactt tgtcttctag actttttgtc tcattgctgc cagttaaggc
     8221 gtaagcagat gcaaaaatta tcttctgggt tggcttgaca cttagaatct catttgcaac
     8281 ctcagaacca ctcttttttg gcatggattg gtctaaaaca accacgtcaa agggattgac
     8341 gtcaagggat tcaaactctt ttcttttcag cttttcttgg tatttttgaa gacattctga
     8401 accgtcctta gttattgtga tgctgtgacc atacctacgt aaaattcggt catattgagt
     8461 cgctgtaaac tggttgtctt cggcaattaa gatgttcaaa ctcgattttt gttgcgattt
     8521 gatgtggttt taactctagt ttgttctttg tgaacagaca tggacatact agaaaatcac
     8581 aaaaagcttg atgacctata actcctatgt ctgtggatga actaatcaag aaggggaaaa
     8641 cactgcttgt tgaaggcaag tttgaggacg ccctctcgta ttttgagcag gcgcttttgc
     8701 tggatcaaaa caatgccgat atctggaatc agaaaggagt gacgttacga agcatgggca
     8761 ggtatgatga tgccttagag tgctttaaca aggcgctaca actcgatcct gccgataaat
     8821 atgcatcata ggtgtgtttt gttttgatga aagacaaatt atatgtgcgc aaacattttt
     8881 ctaaataagt aattgaacaa cgttgaaaaa attttgaatg atgcatcttt gctatgtgaa
     8941 gacggaaaat tcagaaaagc aatcttactg tatagtgaag cgttaagaaa acaaccagat
     9001 aatttggatg tgttaatcga caagggcgtg gcattacaga acctaggtag actaaagcag
     9061 gcactcaggt gctaccaaaa ggcagtatct tttgcaccac aaaacgtcac tgcccttttg
     9121 aacaagggct cagtactgca cagcctaggg cggttccagg aggcaatcgc ctgctatgat
     9181 caggccatct ttgttgacaa aaaatgtgct atggcttttg catacaaagg cctctcgctt
     9241 ggagagcaag ggttagtaaa agaagcatta aagcacttca aaaaagctct gactatagat
     9301 agtagttacg atatggcgaa aataagcaag ggaatcgccc aaaagatact tgatgaaaaa
     9361 aatgaaaatt acaagccagt atccaaaaat taaatctaaa atattgttac atcgccaggt
     9421 gctggctcgt gcttttggcc cttatgatag ctaacaaatt cacggtgggt gctttcttgg
     9481 tgttcccgta gtttttctat ggactcaaag acatcattgc aaaggtaaca cttcggttta
     9541 tgttcgtcaa ctagcggtaa taccatacag gaattaccac tttacctaac ttaacctttg
     9601 agattggtgt ggaatgctgt atctcaagtc agtttatttt atattgacta atttgccaag
     9661 tcgagctgtt gcttcgggaa ctagtcagcg aatcattagc aattgagaaa gcgctagctg
     9721 gagatgagct atcgcacaaa gatggcttgg aattgattaa ccatgacaat cttttcatgg
     9781 tcggagcagc agcagacttg gtaagaaaga aactggtagg ggacaaggtg acgtttgcag
     9841 catcatacta catgaactac acaaacgtct gcgctgcaag ctgccaaatg tgtgcctttt
     9901 acagaaaagg tgatgagaat gatgcttaca cgcttacagc agagcaaatt gaacaaaggg
     9961 tcgcagtggc aaaaaacatg ggcgcaacag aagtccacat tgtaggtggg tttcatccaa
    10021 atctgccgtt agactattac gaaaacatga tgaaaataat taaaaagaat catccagatc
    10081 ttaccataaa ggctttcact gcagcagaga ttttctatct ttccaagctt accaagaatt
    10141 cggtaaaaga aattttattg cggctaaagg aggcaggcct tgatacgatg cctggtggtg
    10201 gagccgaatt gttccaccct gaaataagaa accagatagt tcgtggcaaa tgctcaggcc
    10261 aagaatggct tgacacaata gagcaggcgc acagccttgg aatcaaaagc aatgcaacca
    10321 tgctttatgg acacatcgaa aaaccagagc acataattga ccacctcatc aagatacgcc
    10381 agttacaaaa aaagacagga gggttcatca cattcatccc acttaagttc agtcttgaca
    10441 acacagagct tgaacaacaa aatctcgtaa agcacgagtg ctcatcaata tatgatctaa
    10501 aaataattgc aacctcaaga ttgatgcttg ctggtgcgct cgataacgtg tctgtgtact
    10561 gggttgcata tggaaaaaag cttgcacaag ttgcactgtc taatggtgga agcgacttgg
    10621 ttggcactgc gttttctgag gaaatttacc gggcagcagg taaacccact aattcttcag
    10681 ttttggagct tgcagaaatg gtaaaggaaa ttggcagaca accaattcag cgtgatacct
    10741 attttaggac gattcgaacc ttttaacgat gtttttcgag ggactggatt ttttctttca
    10801 tcgtttgctt tttgtcgttt ctagagtcta tttttagtat tatctcgact cgttttaccc
    10861 caagtctatg aattacctct ataatgtttt tagaagcctc aaagatttgt tctgcacttt
    10921 ctgcctctag aattgttccc attggggtga tttgatgttt gattttgaga tcctttattg
    10981 catcaattgc ctttgctacg taaaagctga cacttgtaga atcggttccg atgggataaa
    11041 cactaatttc tgcgtgaacc atgaattcat ctaatgtgaa atcgattaag tctttatgca
    11101 ggcggtttct tttctatcat ctttgcgccg cctttacgct ttcttgctcc aaaactgatc
    11161 agaactagca aaaatccaac acctgctaac aagccagcca aattggtata atccctgttt
    11221 tcctgctgct ttagcatcag gtctatttcc tgttcttctg tcatttctgg ggtgccaaga
    11281 ggcgaaatgg agtttaggta cgctagcagc gacaaaccaa caatgatcat tgccagcccg
    11341 ccaccaagaa ttcttttatc cacaagtacc attatttcct taccacttta cgattaatta
    11401 aaatatgttt ttgccaaaca cctgtttgga atacaaaaat tttgcttgca agcttgaaac
    11461 gtttgtttat ttttgatgaa tcaattaaac ataatgaaag gttggtactg atcaccttta
    11521 ttttataaaa cgacgattca tacctgaagc taacacacgt acttgtaata tcaaaaggtg
    11581 gtaatggtat tgcatgaaaa tgctgtgacc tgttatttat gcagactgtg ttagcttctt
    11641 tgacttattt ttatatcaaa tggctcgatc aggttttgtt ttactgcaag ctcgaacatc
    11701 tttcttattg ccgcctcgcc tgattggccc atctcaactg tgatgtcatt tacatacatt
    11761 ttgacaaact ttttgataag atcggctggc ttaccccggc tgtatttcat ggcgtactgt
    11821 aatgcttcgt cctgatgctc aagaccatat cgaatcgacg cttgtaggta atcatcgaat
    11881 ttttgtattg tagtgttgtc aaatttgttg ctcatcacat ttgtgccaag aggaactggg
    11941 agaccgtttg tggttttatc ccaccattgt ccaacgtcta gtattttttg caagctttcc
    12001 tgctcgtacg aaagctgggt ttcatgtatt actaggcctg catcaacttt accctcgcgt
    12061 acagcctctg gaatgtcact aaaattcatc tcaaaataat cgaatttgcc aatcattaac
    12121 tgcagaagca aaaatgccga agtcattttt ccaggaattg caatttttaa tgttcggagc
    12181 ttttcatgtg ttatgttttc tttggcaatg acaattggtc catagccaat tccaaaactg
    12241 ccaccactcc gcaaaattgt gtaattggga aggtgtgcac aagcatgaac cgaaacggca
    12301 gtaacgtcaa gctcatactt tagcgccttt ttatttaggg cttcaatgtc ctcaactacg
    12361 tgattaatgg taaactctac cgatggtact ttgcctgtta gcattccata aagcatgaat
    12421 gcatcatcag catctggcgt gtgccctact gagatcttca tgttttagtt ttaaaatttg
    12481 attataaata gcgttatcta aacttgagaa attcaatcat tgagtattag aaaaatttta
    12541 aaaaaatatt aaaattttac aggcaggtat taacctggtc tgtaatgatt ttagattctg
    12601 tatctaaact agttctgcag tagcgaatcg tctgccaact tgcgtgacct tgatttttgc
    12661 ttcttggcca ggctgtccgc cttttacaaa tatgacaaat ccttctactc ttgcgattcc
    12721 gtctccttgt ctgctggttt cggttatgga tacatcgtac tctttaccag tttctactgg
    12781 ttttggagta tcgtcaaact gtctatcgtt aaaacgtcta cgtccaccgc cgccatatcc
    12841 tccaccgcca aatcgtcgac ttggtcgatc ggaattatcg ttaaagctca ttcttacacc
    12901 tccacttcgt cttacttgtg agtaatcagc ctacaatata tcttaacccc ctcaatgatc
    12961 tttaccacat aggtaattac ggcgcactga ggcatcgaaa tcagacaggc tgcagagatg
    13021 gcatcaattt acatgctgct gatactgtct taaacttgga ttttggcaag ttgtcttact
    13081 atgtcttttt ctgtcacaaa accaacgatt ttctgatttt gtgttactgc aacaccatgg
    13141 attctataat cgattaggat cttcgcaagc tttgcaaggc ttgtctgtat tgaaatgctg
    13201 atcagttgtt ttctcattat ctcgcccact gtgcaggtct tgccaaaacc cgtggcaaat
    13261 actgtatcgt cgtatctatt ggagttgtat attgtgtgac gcaaaaaact cttgtatgtc
    13321 acaattccaa ttgatttgcc attttcatct ttgactaaca gacgtgatat ctgattatcg
    13381 agcatctttg tgagtgcgtt atacaacgtg gtagtctgag gcacaaaaaa gcttccaacc
    13441 gacataatgt ctgcaagctt tgtctcatct acgatgtttt gctgaaaaaa cttgactagg
    13501 tcgtgctttg tcatgattcc ctttactccg ctgtgatcct tgacgcctag cgcattggtc
    13561 cgtttgctta acatcagagc agcacagttt gagattggcg catactgatc aaccatgact
    13621 agttcttgca ttttttgcga aactgggata ctgctaatgt tttgtttctc tgagattaat
    13681 gcacctgcga tgtctttttg tgatatgatg tcgtattttt cacgtgaatt gactatgatt
    13741 ccagagatgt tatgcattag gatcttttca gctgcgtcgt aaagtgtgga ggttttgttt
    13801 atgaaaatgg gtgggtgtat gagatcttcg gcagtgacaa gaaaagaacc atattgtcgt
    13861 tgttgtttta gcaataataa caaaattttg cgcaaaacaa tatagtgtag gctgttcgat
    13921 tcacccaaac aaaaatttgg tttttgagca gtaagaactt aaggttgtgg taacaaatcg
    13981 taaatatgaa cacgattgaa gcatatgaaa aagacatcag tctccaagtc ggattcttac
    14041 aaaagataaa actgcaaaaa ccactcagtc agcaacgatt agacaagacg gtgttttgcg
    14101 gaaccggcga ctcgttagct tcagcaatgc ttgcagaatc gttttctaat tttcgagtga
    14161 aggcactgga tccactcgac atcataaaaa ataaaaaact agtacaagga aagtttgcct
    14221 attttgtatc cgtatctggt aacaccatat caaacatcag ggccgcaaaa ctggcaaata
    14281 attccacagc catcacaaaa aacagaatca gcccacttgc caagtcttgc aatcaaccaa
    14341 tacaactaag ataccaagat tcacatgttc tcacgtcagg aagcataggc tttcttgcaa
    14401 gcatgcttat gtgcgtgtca cttgtctttg attttaggat taaatctgca aaaaaactat
    14461 tcatggaggc acaaagccaa gcatgtaaaa tattgctaaa aaataaaata tatgtaattg
    14521 gtaatcaaca tacatacccg cttgccatgt atgcatctgc caaactgtat gaggtattgg
    14581 gctctgatgc acattatgaa agaattgaac aattctcaca tgcaagcttg ttttctgcaa
    14641 aaaaaggcga cactgtaata attttcgaga aaacaaatcg acacaacaaa cagcttgtag
    14701 cccagctaaa aaaacttggc ttatcagtgt ataatccatc aattatgaca gctgacaaga
    14761 tttctcaagt gatcttttac acatttgttt cgcagctcat gacattgtat gatgcaaaaa
    14821 gaaaacagct agatgactgt catttcatca tcgaaaaaaa attcagaaat gcgagctcga
    14881 cgatgattta ctaatttttt ctaaaatcta cgtttcattg acataagatt taatacacct
    14941 tcatcaagga cttgattcaa atatgaagta caaggcaaca gaacagatcc taaagataat
    15001 taaaaacaag gaaaatatca gaaactttgg tgtaatagca cacgttgatc acggaaagac
    15061 aacgatgagt gatagtctct taggatatgc aggcataatt tctccacagg ccgcaggacg
    15121 cgcacttgcc ttggactcga tgaagcttg
//
LOCUS       DASWKH010000050         9736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_850784, whole genome shotgun sequence.
ACCESSION   DASWKH010000050 DASWKH010000000
VERSION     DASWKH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850784"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VGA92_03750"
     CDS             complement(<1..682)
                     /locus_tag="VGA92_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845560.1"
                     /translation="METTAPRPRGVFSYKVLAAIFGLAITWHVINNIVQPEEGTYDTF
                     ELILTLSYPVTAFASFHVAKRYWHSEIFGKAYLSLGLAFLMYFFGDVGYFYQENVMGV
                     EAYPSFADIGYFAFYPFAMYHLLKNSLYFKRKFEMKTKAWISAFPVVMVVSYSILSLS
                     MGGEFNFDFFYGLSFISLNATVLAFAILGAQVFRQSALGPVWGLIVLGMIFYTIADGW
                     YYHLEIFEQ"
     gene            complement(811..3369)
                     /locus_tag="VGA92_03755"
     CDS             complement(811..3369)
                     /locus_tag="VGA92_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEFG-CTERM sorting domain-containing protein"
                     /protein_id="HEX9845561.1"
                     /translation="MADSFEYDMTLPNQYGKGSWPLHIKLSIMNDATNLYFQSTITGP
                     SNVDWHASWAEYRFDKEHDSKYDQNEDMLVMWSSTGFEDLVSNNTDVKNGGKNDGMGA
                     MLNGTTYETIEVMHPLCSGDKLDFCLKVGDRIGFAFGYGVLGTQEFWPSADVTNFADI
                     VIAGDNSKWNVGEVRWVEKFYSAIGTGVVRVNDPNKDLDPEISDNFQIDVWSDSDVEG
                     ITLTLEETDASTGMFEGTVFFTKTDDSSGNKLRVSEGDKITARYVDTTLPNPFTTADK
                     LHIEDIAIIKGKNVWSLGEIEGKNLDLVNNDGIIVFPPNIENYFPVNKIIREKNSKDL
                     IPPGRFSEYEIAKYDGENLTLRKTTRIIIITHDEFVSIFGEDRVIEPLQINTRTVTID
                     DNYILAKINSALSTCSQQNPQKVFCNSSTVELKNLISEKIGTHTINETLVLRKIITYP
                     KDGEITNSTPFASIIPIKYNDNSHGKNEQINVIFPTTDFGVGKIGGQYDPIPFADATT
                     GQFQQSADTYNLTFLNGFTIGYGFEREWSYEYDVLSMIPISADLHIENGLGIGLRIPI
                     QVIMKINPIIQNQQTTNEPEYEVKYTINTLDLDERRYQEIGLPSGQEFAGKEFKIYLG
                     PRLDFVIKIFGDEFFEQDESLVPVDVPTATDFAPPLGTKTTITDYQLPCDWTRTCFDS
                     PLVSGGLYTGAKVDLAGEKITIDSTLLNPIDRNSEKFTFMSIGEQRTQDSMIEVSDNT
                     IISSEDQQYIPYGVELQNVEYRSDLDVIPRIMLQTKINPWIFPLNLSTDWIDLPGIQF
                     SEVVFETHANTDGIFKTTDGKYVVPEFGMITVIILAFALVSITLINKVQSLRMN"
     gene            complement(3617..5698)
                     /locus_tag="VGA92_03760"
     CDS             complement(3617..5698)
                     /locus_tag="VGA92_03760"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX9845562.1"
                     /translation="MELQQILPIGAAIASFAVAAGYAVWVGKQKAGTKEMMEISDAVR
                     VGASAFLRREMKIIIPIAIGLSVIIGYFIGFSNGVAFAVGAALSAVAGLISLKITVKA
                     AVRAANATGSGIGKTFALAFRGGATVGLAVPAMALLALALLYMAFPDPITIAGVGIGA
                     SLIALFIRIGGGIFTKAADMGADLVGKVEANIPEDDPRNPATIADNVGDNVGDAAGMG
                     SDVYESYIVTVLASILIGALIGMPKIVIYPILIGASGLIASIIGSATIGSKDHADVMK
                     PLNRSFYVSAAIAISLNFVFTYFFIGQDRLSYALFGSTVVGVILVPVIQRITDYYTNY
                     KYRPVIEIAESAKWGYASLTLMGIIKGMQSTGPFMIALVVALIVSFSISAAAAPDPSQ
                     ALIYGIFGTSLTAMAMLSLAGIVLSIDAFGPIADNAGGIVEMTGMGDENRKVTDQIDA
                     VGNTTKAVTKGFAIASAGLAALAMIQAFQYEAKQFLAEHATTTFAHLSPENFAKLSDY
                     TLTNPAIIIGLLVGSLIPFIITGQLISGVSRAASKMVDEVRRQFKADPEILTGKSKPD
                     YAKCVDIATVASLKELWKSASIAIAAPIVLGILLGPPAVAGLLMGAVVTGIFLAFHLA
                     NTGGAWDNAKKLIEMRGQKGTEEHKVAVVGDIIGDPYKDTAGPALNTVIKLLNTVAIV
                     FVSAFVAILVL"
     gene            complement(5823..6191)
                     /locus_tag="VGA92_03765"
     CDS             complement(5823..6191)
                     /locus_tag="VGA92_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845563.1"
                     /translation="MKLDDDLRLSILEKVGIFSSRLAISEPRTLLSTAEVLGMPKELT
                     VGRRTTAYKYYGVSYMQHNLVFINVKKIPDEKSLDDTIAHELVHLRFPYLSHGRRFNK
                     LVRQVLKGKTFAPYQKRKMH"
     gene            complement(6476..6859)
                     /locus_tag="VGA92_03770"
     CDS             complement(6476..6859)
                     /locus_tag="VGA92_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845564.1"
                     /translation="MTKNPTVIVVEDDENMLTVFTEWIRMSGISVLATGTDGKDAVEL
                     FDELKPDVAIVDLMMPEYDGFYAILGIREIDPNAKILVLTADVTEKTKRRLDQLKSIS
                     LMYKPYDLDDVVHEVKRLVSETITV"
     gene            7031..8167
                     /locus_tag="VGA92_03775"
     CDS             7031..8167
                     /locus_tag="VGA92_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9845565.1"
                     /translation="MRKFIVRKDGDSELSLQTKSVDPMSFTERSVFFKKDSQNNASED
                     TLEQESEDLKSNKGQQTSEFADVVEKENQDGDSDDRLAQSKEIKKLFDESLYAVKAFT
                     ENKTKLLQKNLEEEKQLTKQLNETLQQNLLKISKIDEDLEHRKNHLEVEVDEKTKKLL
                     ASEKMTAIGELSARLAHDIKNPLTMIKNTVKILRTQEGKQIDEYVLKRFDMVDDSIFR
                     ISHQIDGVLDYLKCTPLKTAPASLVGILKSSILPLSIPANVKINFPSADLTLKCDAIK
                     MEIVFGNILLNSLQAIADTQGQIYVRFAKKPGSVIIELVDSGPGIAQEHIEKIFDPLF
                     TTKQKGTGLGLTSCKNIIDQHGGKISAKTNPTTFTIVLPAPETK"
     gene            8241..8330
                     /locus_tag="VGA92_03780"
     tRNA            8241..8330
                     /locus_tag="VGA92_03780"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8276..8278,aa:Phe,seq:gaa)
     gene            8711..>9736
                     /locus_tag="VGA92_03785"
     CDS             8711..>9736
                     /locus_tag="VGA92_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845566.1"
                     /translation="MGKNSINIPIIFANNLPKVDTKFATLALLVFGFVVLSSAQDAHA
                     ATFTAVQSGNWNDPATWGGQVPDIGDDKVIPAGIVVEVSAIILLTNSGTITNDGTIDN
                     LISGTITNSGTINNSGTINNRLSTLTNSGGTINNSGTINNVFGTLNNNPLSTITNSGT
                     ITNSGTITNSGTITNSGFIRNSNTINNNSGTITNSGVIFNTSGGIITNLSTITNNSGG
                     TIENSGTINNPSGTITNSGTLTNTGVTIFSSVFGTITNTGTITNLNTINNGRDATITS
                     TGTFKHVFPLASYSGSGTFTGHVFLVGTIPDDVALGSGSLSYPSSVSTYLISSQDTLT
                     IDAGDTLT"
BASE COUNT         2823 a         2150 c         1865 g         2898 t
ORIGIN      
        1 actgttcaaa gatctcaaga tgatagtacc acccatcagc aattgtatag aagatcatgc
       61 ccaacacgat caatccccat acgggtccta gggcgctttg acgaaatacc tgggccccca
      121 agatggcaaa tgcaagtacg gtggcattaa gtgagataaa agaaagtcca tagaaaaaat
      181 caaaattgaa ttcaccaccc atggataaag aaagtatcga atatgataca accattacaa
      241 ctggaaatgc agatatccac gcctttgtct tcatttcgaa tttgcgctta aaatataggc
      301 tatttttgag caaatgatac atggcaaagg gatagaatgc aaagtatcca atgtctgcaa
      361 acgaaggata tgcttctaca cccataacat tctcttgata gaaataaccc acatcaccaa
      421 aaaaatacat cagaaatgct agtcctaaag aaaggtatgc ctttccaaat atttcagaat
      481 gccaatatct tttagcaaca tgaaatgagg cgaacgcggt tacaggatag ctcaaagtca
      541 gaattaattc aaaggtgtcg taggtacctt cctctggttg aacaatgttg ttgattacat
      601 gccatgttat tgcaagacca aatatggctg caagtacttt gtaactaaac acacctcttg
      661 gtcttggcgc agttgtctcc aaattgtaag taatgggtat tattaagtag ttaaagttct
      721 cgttagatcc taagttatga aattcttatt tgacaaagca agtatgttgc gtcttaaaca
      781 acaaaatgaa ctgatcaact ttttgaaatc ttagttcata cgtaaggatt ggactttgtt
      841 tatcagtgtt atactaacga gcgcaaatgc tagtataatc acagtaatca ttccaaattc
      901 tgggacaaca tatttgccat ctgttgtttt gaatattcca tcagtattag catgtgtttc
      961 aaataccact tcgctaaatt ggattccagg caagtctatc cagtctgtgg acaagtttag
     1021 tggaaaaatc caaggattta ttttagtttg caacataatt cttggtataa catccaaatc
     1081 tgatcggtat tctacatttt gtagttctac tccgtacgga atgtattgtt gatcttcaga
     1141 ggatattatt gtattgtcac taacttcaat cattgaatcc tgagttcttt gttcgcctat
     1201 gctcataaat gtgaatttct ctgaatttct gtctatggga tttaataatg tactatcaat
     1261 tgttattttt tcacctgcta aatcaacttt ggctccagtg tataatccac cactgactaa
     1321 tggagaatca aaacatgtac gcgtccaatc acacggtaat tggtaatcag tgatcgtagt
     1381 ctttgtacct agtggaggtg caaaatctgt ggcggttggt acatcaactg gaactaaact
     1441 ttcatcttgt tcaaaaaatt catctccaaa tatttttatc acaaaatcta accgtgggcc
     1501 caaatagatt ttaaactctt ttccagcaaa ttcctgtcct gatggcaatc caatttcttg
     1561 atatcttcgc tcgtcaagat ccagcgtgtt gatcgtgtat ttcacttcat attctggttc
     1621 attagttgtc tgttgatttt gtattattgg attaattttc attattactt gtattggtat
     1681 tcgtaagcct attcctaacc cattttcgat atgcaaatca gcagaaattg gtatcatgct
     1741 taaaacatca tattcatagg accattctct ttcaaatccg tatcctatag taaacccatt
     1801 aagaaaagtt agattgtatg tgtcggcaga ttgttgaaat tgtccagtgg tggcatcggc
     1861 aaatggaatt gggtcatact ggccaccaat tttacctacg ccaaaatctg ttgtaggaaa
     1921 aatcacattg atctgttcgt tcttcccatg actgttatca ttatacttga tgggtataat
     1981 tgatgcaaaa ggagttgaat ttgttatttc gccatctttt gggtatgtta tgatttttct
     2041 caataccaga gtttcattaa tggtatgggt tcctattttt tctgagatta gattttttag
     2101 ttcaacagta ctgctattgc aaaacacttt ttgaggattt tgttgtgagc aggttgatag
     2161 tgctgaattt atctttgcaa ggatatagtt gtcatctatt gttactgtgc gagtgttgat
     2221 ctgtaatggt tctatcacac gatcctcacc aaatatagaa acaaattcat catgagtgat
     2281 tataatgatc cttgttgttt ttctaagcgt tagattctct ccatcatact tcgctatttc
     2341 atattcactg aatcttccgg gaggaattag gtctttggaa tttttctcac gtatgatttt
     2401 gtttacggga aagtaattct caatatttgg tgggaaaaca attattccat cattgttcac
     2461 aagatctaga tttttccctt caatctcccc taaactccat acattcttgc ctttaatgat
     2521 ggctatatct tcgatatgga gtttatctgc agtcgtgaat gggttaggta gtgttgtgtc
     2581 tacatatctt gcagttattt tatcaccttc tgagactcta agtttatttc cagatgaatc
     2641 gtcggttttg gtgaaaaata cagtcccttc aaacattcca gtagatgcgt ctgtttcttc
     2701 taacgtaagt gtaatgcctt caacatctga gtctgaccag acatcgattt ggaagttatc
     2761 cgatatctct ggatctaggt ccttgttagg atcatttaca cgtactactc cagttcctat
     2821 tgctgaatag aatttctcaa cccatcgtac ctcccctaca ttccattttg aattgtcacc
     2881 tgcaattaca atgtctgcaa agttagtaac atctgctgat ggccaaaatt cttgagttcc
     2941 caaaactcca taaccaaacg caaatcctat tctgtccccc actttcaaac aaaagtccag
     3001 tttatcccca gaacatagtg gatgcataac ttctatagtt tcgtacgtgg tgccattcaa
     3061 cattgctccc atcccatcat ttttccctcc attttttaca tcagtattat tactcactag
     3121 atcttcaaat cctgtagatg accacataac caacatatct tcattttggt catattttga
     3181 atcatgttct ttatcaaatc tatattctgc ccagctagca tgccagtcaa cattggatgg
     3241 tccagtaatt gtagactgaa aatacaaatt tgtcgcatca ttcattatgg ataattttat
     3301 gtgtaaaggc caagatcctt tcccatattg atttggcaac gtcatgtcat attcaaacga
     3361 atctgccatc tcccactcac ctacgctgga aatcccatta actttaggtg cagaattggt
     3421 atatgcatga aaagttttgc tagagtggcc ttcagcgaaa taaaattgga ttgggaacaa
     3481 tatcgagaca aaaaataata acgcaaatag tatgaatatt ttcatgagac gttctggcga
     3541 taatggtatt ttagttttct atgactatct tattatcata ttagcataat gcctttgata
     3601 aaactaaatt ttgactctac aaaactagta tggcaacaaa cgcagaaaca aacacgattg
     3661 caaccgtgtt tagcaatttt atcacagtgt tcagagcagg ccctgctgta tccttgtatg
     3721 ggtcaccgat gatatcgcct actacggcaa ctttgtgctc ttctgttcct ttctgacctc
     3781 tcatttctat gagttttttg gcattatccc aagctcctcc tgtgtttgcc aggtggaacg
     3841 ccaaaaatat tccagttact actgcaccca tcaataatcc tgctactgcc gggggtccaa
     3901 gtaatattcc caaaacaatc ggagcagcaa ttgcaatgct tgcagacttc cacagctcct
     3961 ttaacgatgc gactgtggca atatcaacac atttggcgta gtcgggcttt gacttgccgg
     4021 taagaatttc ggggtctgct ttgaactggc gcctgacctc atctaccatc tttgatgccg
     4081 cgcgagacac gccggaaatg agctggccag taatgatgaa cggaatcaga ctgccaacaa
     4141 gcaacccgat tatgattgcc gggtttgtca gggtatagtc agataatttg gcaaagtttt
     4201 ccggcgaaag atgtgcaaac gtagttgtcg catgttctgc caagaattgt tttgcctcgt
     4261 actggaatgc ctggatcatt gccagtgcag caagacctgc acttgctatt gcaaacccct
     4321 ttgtcactgc ctttgttgtg tttccgactg cgtcaatttg gtcggtgacc ttgcggttct
     4381 catcgcccat gccagtcatc tccactatgc ctcccgcgtt atcggcaatc ggcccaaacg
     4441 cgtcaatgga tagtacgatt ccagcaaggc tcagcatcgc cattgcagta agggatgtgc
     4501 caaagatccc ataaatgagg gcctgtgatg ggtctggagc agcagctgca gaaatagaaa
     4561 atgatacgat taacgccaca actagtgcaa tcatgaacgg gccagtcgac tgcatcccct
     4621 ttatgattcc cattaacgta agtgatgcat agccccactt ggcagactcg gcaatctcga
     4681 tgacaggtct gtacttgtag ttggtgtagt aatcggtgat cctctgaatt actgggacga
     4741 gtatgacgcc gactacggtg ctgccaaaga gtgcgtatga gagtctgtcc tggccgataa
     4801 aaaaatacgt aaacacaaag tttagcgata tggcaattgc tgcagaaaca taaaatgagc
     4861 ggttgagcgg cttcatcaca tcggcgtgat cctttgagcc aatggttgca gagccgataa
     4921 ttgacgcaat gagtcctgag gctccaatca agattgggta aattacaatt tttggcatgc
     4981 caattagtgc gccaatgaga attgatgcaa gcactgtgac aatgtatgac tcgtatacat
     5041 cggagcccat gccggctgca tcgcctacgt tatcgccaac gttatctgca attgttgccg
     5101 ggttccttgg gtcgtcttct ggtatgtttg cttcgacttt gcctaccagg tccgcaccca
     5161 tgtctgctgc ctttgtaaaa attccaccac cgattctgat gaatagtgcg atgaggcttg
     5221 ctcctattcc aactcctgca attgtgattg ggtctggaaa tgccatgtat agtaatgcta
     5281 gcgcaagtag tgccattgca ggaactgcca agccgacagt ggcccctccc ctaaatgcca
     5341 gggcaaacgt ctttccaatt ccggagcctg ttgcgtttgc agcccgaact gccgccttta
     5401 ccgttatctt tagtgagatt aaaccagcaa ctgctgaaag tgcagcgccc actgcaaatg
     5461 ccacgccgtt tgaaaaacca ataaagtatc caattatcac tgagaggcct attgcgatag
     5521 gtatgattat tttcatttct cgcctcaaaa acgccgacgc gccaaccctt actgcatcag
     5581 agatttccat catttccttg gtaccagctt tttgtttgcc aacccaaaca gcatagcctg
     5641 ctgcgacggc aaaagatgcg atagctgcac cgataggaag gatttgttgc agctccattt
     5701 acttgcaagc ctaaacctag gaaatataaa gctactatct cgtctgctgg aaaaaacatc
     5761 ttattttgga tggttttgca agatccttcg atcttagatt tggaaattct tagaatgata
     5821 aatcagtgca tctttctttt ctggtatggg gcaaatgttt taccttttag tacctgtcgt
     5881 accagcttgt tgaatcgcct tccatggctt agatatggga accgcaggtg caccaactca
     5941 tgtgcaatgg tgtcatcaag tgatttttcg tctgggattt ttttgacatt tatgaagact
     6001 agattgtgct gcatgtatga tacgccgtaa tacttgtatg ctgtcgtcct tcttcctacg
     6061 gtcagctcct ttggcatgcc aaggacttcg gctgttgaca gcagagttct tggctctgaa
     6121 atggcgagtc tgctagaaaa gattcctacc ttttctagga tacttagtcg taggtcgtca
     6181 tccagcttca ttaatcaagg ttttagaatc gatgtttatc tagcagtgtc aattcttgtg
     6241 caaaaatttg ttgaaaaatt aatccgattg cttgagccaa acattctcaa gatttgagaa
     6301 acgttaggca gtgttttatc agctaaacac aaaaaaatct tggtggggtc agtaaccaat
     6361 accctgacta tctgaaaaat ggttttatcc taggctcaga tgtcgtgggc tcaaatccca
     6421 ctcctaccaa ccttatggtt tctaattata taaagttcgt tgatgttttt aaaaatcaga
     6481 ctgtaatagt ttcgctgaca agccttttga cttcgtgcac cacgtcatcc aagtcatatg
     6541 gcttgtacat tagggaaatc gattttaact ggtccagtct cctctttgtt ttttctgtga
     6601 cgtctgcagt taaaactaga atctttgcat tcgggtcgat ttctcgtatt cctaatattg
     6661 cataaaatcc gtcgtattct ggcatcataa gatctactat ggcgacatcc ggcttgagct
     6721 catcaaacag ctcgactgcg tcctttccgt ctgtgcctgt ggccaaaacg gagatgcctg
     6781 acattcgtat ccattccgta aataccgtca gcatgttctc atcgtcttct actactatga
     6841 cagtggggtt tttggtcact ttattttcta ggatcaaaac agaattaaca ttgtgtgtgt
     6901 gcaaaaaaat caaatatttt gccctgttgc cgcatggtct ttaacaaaga ctgcattaat
     6961 tatgaagtgc atactttgtt tgttcaaata aaaagacaaa tcccttttaa aaaagccacg
     7021 cattgtctgt gtgcgcaagt tcatagtcag aaaggatggc gattcggagc tatcactcca
     7081 aaccaaaagt gtggatccaa tgagctttac agagcgtagc gtgtttttca aaaaagactc
     7141 acaaaacaat gcctcagaag acactcttga acaagaatct gaggatttaa aatcaaacaa
     7201 agggcaacaa acatctgaat ttgcagatgt tgttgaaaaa gaaaaccagg atggtgactc
     7261 agatgaccga ctggcgcagt caaaagagat aaaaaaatta tttgacgagt cgctttacgc
     7321 cgtaaaggcg tttactgaaa acaagacaaa gctactacaa aaaaacctcg aagaagaaaa
     7381 acagctaact aagcaactaa acgagacact tcagcaaaac ctactcaaga tttcaaaaat
     7441 agacgaagac ctagagcatc gcaaaaacca cctagaagta gaggtggacg aaaagacaaa
     7501 aaaactgctt gcgtcagaaa aaatgactgc aataggtgag ctttcggcta ggcttgcaca
     7561 cgacataaaa aacccactga cgatgatcaa aaacactgta aagattctta ggactcagga
     7621 gggcaagcag atagacgagt atgtcctcaa gcgatttgac atggtggatg actccatttt
     7681 tcgtattagc caccaaatcg atggggtact ggactatctc aagtgcaccc cgctaaagac
     7741 ggctcctgca tcgctagttg gcattctcaa gtcatcaatt ctgcccctga gcataccggc
     7801 taacgtcaag atcaattttc caagcgcgga cttgactctg aagtgcgacg caatcaaaat
     7861 ggaaatagtt tttggaaata tcctgctcaa ctcgcttcag gccattgcgg acacgcaagg
     7921 ccagatatac gtcaggtttg caaaaaaacc aggttcggta ataatcgagt tggtggactc
     7981 agggccagga attgcgcaag aacacatcga aaagattttt gatcccttgt tcacaacaaa
     8041 gcaaaaaggg actggccttg gcctgacgag ctgcaaaaac atcatagatc agcacggtgg
     8101 aaaaatatct gcaaaaacca atccaacaac atttacaatt gttttgcctg cacctgaaac
     8161 aaaataatca tctgttaaaa gatctcaaaa tctgagaatc tatcaatgat gttttattgg
     8221 taaaaaacaa ttggaatcac gtaagggtcg gcaagaccgg ttatgccgag cgtctgaaaa
     8281 acgctgatcc gtttcacccg gatgcgtggg ttcaattccc accccttacg ggtttctgat
     8341 attattatgt cttggtcgtt aatatctttt aaaaattctg tatagtatgt gggaattctt
     8401 taagaaattt tatcagtttt gtacgattaa gatctgttca atgacgattt gtgtgtaatg
     8461 gatatgcatt aaacagacaa ttcatagtat gggtcaggac ggatttaatc tagttaatgg
     8521 cctgagaaat ttacaaaaga taagcaaaac ttttcagtga atagtttgga gctaaaaaca
     8581 cgaagtattt tggaattttt actaaaacta ttctagcttt atttgtggtt atttgtggtt
     8641 atttgtggtt attgttttat aaaaattgat ccgttttata taacattatt ctagcacctc
     8701 actactggcc ttggggaaaa attcaatcaa cattccaatc atctttgcaa ataatttgcc
     8761 caaagtagac accaaatttg caaccttagc cctgcttgtc tttggctttg ttgttttatc
     8821 ttctgcacaa gatgctcatg cagctacatt tactgccgta caaagcggaa actggaatga
     8881 tcctgctacg tggggtggac aagtcccaga tattggcgat gataaggtga ttcctgctgg
     8941 cattgttgtc gaagtatctg ccataattct tctcaccaac tccggtacaa ttaccaacga
     9001 cggcaccatc gacaatctca tctccggcac cataaccaac tccggcacca tcaacaactc
     9061 cggcacaatc aataatagac ttagcactct caccaactct ggcggcacca tcaacaactc
     9121 cggcacaatc aataacgtct ttggcacctt gaacaacaac cccctcagca ccataaccaa
     9181 ctccggcacc ataaccaact ccggcaccat aaccaactcc ggcaccatca ccaattccgg
     9241 ctttatccgc aactcaaaca ccatcaacaa caactccggt accataacca actccggcgt
     9301 catcttcaac acctccggcg gcatcataac caatctgagc accataacca acaactccgg
     9361 tggcaccata gaaaactccg gcaccatcaa caacccctcc ggcaccataa ccaactccgg
     9421 caccttgacc aacaccggcg tcaccatctt cagctccgtc tttggcacca taaccaacac
     9481 cggcaccata accaacttaa acaccatcaa caacgggagg gacgcaacca tcaccagcac
     9541 cggtacattc aaacacgttt tcccactagc gtcatattct ggctccggca cctttacagg
     9601 acatgtgttc ctggtaggca ccattccaga tgatgtcgca ttgggttctg gctcgttgtc
     9661 atatccaagc tcagtcagca catatttgat atcctctcaa gatacgctta ccattgatgc
     9721 tggggatacg ttaacc
//
LOCUS       DASWKH010000051         9491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3427902, whole genome shotgun sequence.
ACCESSION   DASWKH010000051 DASWKH010000000
VERSION     DASWKH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427902"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGA92_03790"
     CDS             <1..385
                     /locus_tag="VGA92_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845567.1"
                     /translation="RALQEKGMPYYQLTNKGLLVSVALDEIIGREKILEKFFTQHHDV
                     DKEFQGQIETLVKFAPRFVYSLFRSYVKAYCDGRIDDLLPFDKTRFMQVSDETILIQK
                     EFLESFIVMSKSEKEKALNFLKSIN"
     gene            450..1499
                     /locus_tag="VGA92_03795"
     CDS             450..1499
                     /locus_tag="VGA92_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX9845568.1"
                     /translation="MASAGSQKIFASVKSFSQRGRLLTRAELQTLAESRDLDELITRI
                     KNTKYADAISKISKPFTAAKVESALRSKLADIHYSISKTAGHSDILDVYYLKFIITNL
                     KQILKGKALGKSQEEIEPHLNLHAEELINQRDIVVKALVSKDLEEAVTTLGAIEFGEE
                     VAKAAALYNEKKNVQIFDVFLDKILYQQLGRAVRISRDRDVVRLAGMDIDFYNILSIL
                     RGKFWGLDEEQIKNLIVTHTPSIPQDLLGKMISCDSVKNVLDELLTTRYKDLVPQTED
                     SIEAISQFEHSFEMAIYKAVNRSFTKMFSFATIIGITKLTAYEVRNISAIAFAVEQKI
                     APQTTMSRLIIEEQG"
     gene            1539..1775
                     /locus_tag="VGA92_03800"
     CDS             1539..1775
                     /locus_tag="VGA92_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845569.1"
                     /translation="MIRIHKKKTEITAEVLVNTVWVSTFLALILTIPALGVFLGIYFT
                     TSNLLVGAVIGFGLHFVTLAFSDRISKQLTRLLS"
     gene            1792..2436
                     /locus_tag="VGA92_03805"
     CDS             1792..2436
                     /locus_tag="VGA92_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845570.1"
                     /translation="MMGDRNYRDTVSSAIDAITKETEMQLSNPQDNKTVFLHEVVRCL
                     RRSYFDRFDRLEPENRVFGSLFEGLIRKLPYGSKVGEFAIDEIKFKGQADMIVDDIVI
                     LFKMVSSLPEMPFASDILYLNACMWIFNKTDGVVIYITNDGGESSFSVLREKKMFEEI
                     LRRVRVFSNLIGEKKAPILEPSLECSGCQYYERCYIKRHEGKQFSLSEMFSIKK"
     gene            complement(2666..3244)
                     /locus_tag="VGA92_03810"
     CDS             complement(2666..3244)
                     /locus_tag="VGA92_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845571.1"
                     /translation="MLLSSYSANNLIHAAEFLLDLTKTLNGEITNLQKLKDEVEQQQE
                     EAHKQGQIVKKMTNYLSDELGRLELRTDNSPVLLGSNLEKLTQTETKLRLLRGRLTDF
                     VESDDANNLKKSDSPLADALKHYESFLLELTNTLNEKIPQLEKIIHRIRGESREAQQQ
                     KLLLKEIVELFNDELEELDLAFCELKEKTSVS"
     gene            complement(3304..3849)
                     /locus_tag="VGA92_03815"
     CDS             complement(3304..3849)
                     /locus_tag="VGA92_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845572.1"
                     /translation="MNSKVLLGIIAAALAGIIGFIAISGSQIINDVSEGGLVRTSSSP
                     IQVLPLEVQLEKLSILSVSEQEATLQIDFKITNPNYKSVILQVIKYELYDGSKRITIE
                     QIGERPEGMVDSSNYFTILSNNPQIIGETFTIKNSGNAPEFWSSLSNNDIKWTIKGEA
                     YFNLSSMTSGQENIISFEFEA"
     gene            3925..4278
                     /locus_tag="VGA92_03820"
     CDS             3925..4278
                     /locus_tag="VGA92_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845573.1"
                     /translation="MQYFQAVQIGKERSSKSQMILFKLGGFAMLTLTTKKKEGQFIPV
                     GEEDLVAVIKTNDGYVTILVDSEGYTKAQSKPLDKDAAMEIFKKARESGIEEFVGNKV
                     EIWTEQYPTVQNELK"
     gene            complement(4281..4961)
                     /gene="pyrH"
                     /locus_tag="VGA92_03825"
     CDS             complement(4281..4961)
                     /gene="pyrH"
                     /locus_tag="VGA92_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX9845574.1"
                     /translation="MTKKRIVIKLSGRIFEMDNNTKLLKDYATFLVKISKICQPVVIA
                     GGGKIARHYITHARSSGADESTLDELGIEVSRLNARLLIYALKTKAYPHPPTTLTEIR
                     HAVDSGLIVIAGGLHPGQSTNGTAALIAEKIGATEFLNATDVDGIYDSDPNKNPKAKK
                     FKRIELKDLRRLLVREDSVAGGYDLMDIVALKVIERSKIKTRVIKADIQTLEKAIKGQ
                     PVGTEIAL"
     gene            complement(4961..6196)
                     /locus_tag="VGA92_03830"
     CDS             complement(4961..6196)
                     /locus_tag="VGA92_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9845575.1"
                     /translation="MTKPFLDIVDPIHDFIRVYETELKIIDSPIFQRLRRIRQLSGAH
                     FTYPSAQHSRFEHSLGVMHVAGQAAQALKEKGFLNTDKIQEIRLAALLHDIGHGPFSH
                     LFEEVLQTKKKTSHEEIGKKIILRSEIGEILSAAGFDKKFITKLAFGDSKYRFVNEII
                     SGSLSADMMDYLQRDGYFTGAEHAKIDHKRIIQSLDVYKNKLALDRSALYSFESMILS
                     RYQMFKAVYFHKTVRSAEVMMLEALRLADDEANFTSLNLDDYVKLTDEFVISQILSLP
                     EYTQDLRRAKKIATDYQNRRLLKCVYEQIMTNKKLKDQDKIRQKIAKKSKVDINEIFV
                     DTSTTSSLPLTPSKEKTKSIILMTKYATKSTAKEIPFSKIPVVSSMAESMNILRIYTT
                     LQHRKKVEMAAELILGDKR"
     gene            complement(6193..6777)
                     /gene="tmk"
                     /locus_tag="VGA92_03835"
     CDS             complement(6193..6777)
                     /gene="tmk"
                     /locus_tag="VGA92_03835"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX9845576.1"
                     /translation="MIIVIEGTDQAGKKTQSELLACVLKKSKIKTKIFSFPDYTTPIG
                     KEISRFLHGKRKFPPQVIHCLLAANRWEKLDQIKKAHSENSVLIMNRYYQSNLVYGVA
                     NGLSLKWLEGLDAGLPKADLVIVLDVSQRESFSRKKTNRDKFEKNTFFLNKISKTYRQ
                     LAKKQGWQIVDASGTKNHIHQSIMKILSKKLAKL"
     gene            6953..8119
                     /locus_tag="VGA92_03840"
     CDS             6953..8119
                     /locus_tag="VGA92_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9845577.1"
                     /translation="MQRTFKFRLYPNSIQTKQLHYTLGACRWVYNKMIEKIRSEGLHT
                     RNDLNYFLTELKEQDRWLYSYHSKMLQMVSTQADGAQKSLAQLRKNGHKTGDLKFARF
                     SEYNTFVYNQSGFEIKDSFLHLSKIGRIKIIQYRDIPENSEIKQITISKSKSGKWHAC
                     VIADIDAVLPKISLEKSVGIDVGIKNFAYDSDGYQTPNPLNLQKMLRPLARAHRKISR
                     RQLGSQNRKKAVKWYQIVHERIKNKRRDFLHKLSTLYARKYDVVFLERLTKLNMVKNH
                     RMARSILDSGWGIFGNMLDYKTLLIEVPSKNTTVNCSRCGNMVPKSLAVRTHQCDRCG
                     LVLDRDHNASINILKKGLEIFGITHQIQQELREVTPVEITKWSMKQEALSFNEG"
     gene            8130..8516
                     /gene="hsp14"
                     /locus_tag="VGA92_03845"
     CDS             8130..8516
                     /gene="hsp14"
                     /locus_tag="VGA92_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HEX9845578.1"
                     /translation="MGIIKYMAKEMTKEIGNKSREFYEFVLPPVDMYLDEDSLTVVVD
                     LPGFDKKDIKLTLHRSILSISAEKHDSQKDNAVYRQRPNIIDKKILLPIKVEEDKEGV
                     VSAKFVQGVLTVKIPASHQGKQISIE"
     gene            complement(8517..>9491)
                     /locus_tag="VGA92_03850"
     CDS             complement(8517..>9491)
                     /locus_tag="VGA92_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX9845579.1"
                     /translation="KESSNLKKIKRNLERHDNVIIPQVYDDHTSKNVLTLEYIPGIKI
                     TNIEDLDKKGIDRQKIVIDVHKVFFTMLLRDSIFHADPHPGNISVTDDGSLILYDFGM
                     VGRIDQETRMRLVRLYLALVEKDPPRTVNAMNDLGMLMPDFNRSVIEQGIDLSIRTMH
                     GKKPDEMEVRALLELANKTMSKFPFTLPKNLALYLRMSSIIEGIYKTHKVDFRFVKVL
                     KNILEEEQLIKDAYIEELKYSFTRFAKSLDATISIAPELKKFLEENRSLQLLNARPKS
                     NTLLAGSILSSAIFIGSTLLYSSNETAGISGMISSLIIMGIFAIFRKY"
BASE COUNT         2910 a         1713 c         1908 g         2960 t
ORIGIN      
        1 tagggcattg caagaaaaag gcatgcctta ctatcagctt acaaacaaag ggcttttggt
       61 ttcggtagca ctagatgaga ttattggtag ggagaagatt ttggagaaat tttttactca
      121 acaccatgat gtcgataaag aatttcaggg acaaattgaa actttggtaa aatttgctcc
      181 ccgatttgtt tattctttgt ttagaagcta tgttaaggca tattgtgatg gtaggattga
      241 cgatctttta ccatttgaca agacacgatt tatgcaagta tcagatgaga caattttgat
      301 tcagaaggaa tttctcgaaa gttttattgt catgtccaaa tcagaaaagg aaaaagcgtt
      361 gaattttctt aagagcatca actaaatttc aaataatttg gcaaaaattc cccaaacatt
      421 aaaatccatt gtaaatacac gacaaaataa tggcttctgc cggttcacag aagatttttg
      481 catcagtaaa atcatttagc caacgtggaa gactgctaac aagggcagaa ctccagactt
      541 tggcagaatc gagagatttg gatgagctca ttacaagaat aaaaaacacg aaatatgctg
      601 atgcaatatc taagatatca aagcctttta ctgcagccaa agtcgaatct gcgcttagaa
      661 gtaagcttgc agatatccat tattcaatta gcaaaactgc cggtcactct gatattttgg
      721 acgtatatta tcttaaattc atcattacca acctaaagca gatcctgaag ggaaaggcac
      781 ttggtaaatc acaagaagag attgagcctc accttaacct gcatgcagaa gagctcatta
      841 accaaagaga cattgttgta aaagccctcg tttcaaagga tcttgaagaa gcagttacaa
      901 cccttggtgc aattgagttt ggtgaggaag tagcaaaagc agctgcgcta tacaatgaga
      961 aaaaaaatgt ccaaatcttt gatgtttttc ttgacaaaat tttgtatcag caacttggtc
     1021 gtgccgttcg aatttctagg gacagagacg tagtcaggtt agctggaatg gacattgact
     1081 tttataacat actttcaatt ttgaggggca agttttgggg tctagatgaa gaacagataa
     1141 agaacctcat tgtaacccac actccaagca ttccgcaaga tttacttgga aagatgatct
     1201 cttgtgattc tgtaaaaaat gttctagatg agctcctaac gactcgctac aaggatttag
     1261 tgccacaaac agaagacagt attgaggcaa tatcgcagtt tgagcattcg tttgagatgg
     1321 caatctacaa ggcagtaaac agatcattta ccaaaatgtt cagctttgca acaattattg
     1381 gaatcacaaa gcttactgcc tatgaagtaa gaaacatttc tgcaattgcc tttgcagttg
     1441 agcaaaaaat cgcgccacaa acaacgatgt caaggctcat aatagaagaa caagggtaat
     1501 ctgcaattat atatggtaca gggctcgtaa agccattaat gataaggatt cacaaaaaga
     1561 aaaccgaaat tactgccgag gtattggtaa acactgtttg ggttagcacc tttttggcct
     1621 taattcttac aattccagca cttggcgtgt ttttagggat ctattttact acatcaaatc
     1681 ttctagtcgg tgcagtaatt ggctttggct tgcactttgt cacacttgca ttttccgata
     1741 gaatttcaaa gcaacttacg aggcttctca gctaaggagt gaaaaagcat catgatgggg
     1801 gacagaaact atagggatac cgtttctagc gccattgatg caataacaaa agaaacagag
     1861 atgcagctgt ccaatccgca agacaacaag acagttttcc tgcatgaggt tgttcgatgt
     1921 ttgaggagat cttattttga taggtttgat agattagaac cagaaaacag ggtttttggt
     1981 agtttatttg aaggcctaat tagaaagcta ccatatggct ccaaggtcgg cgagtttgcc
     2041 atagatgaga tcaagttcaa aggtcaagca gatatgatag tagacgatat tgtaattctc
     2101 ttcaagatgg tttcctctct tcctgaaatg ccctttgcgt cagatatttt gtatcttaat
     2161 gcatgtatgt ggatttttaa caagactgac ggcgtagtca tatacatcac aaatgatgga
     2221 ggagagtcgt ctttttcagt attaagggaa aagaagatgt ttgaggagat actgcgtcga
     2281 gtgcgagtgt ttagcaatct gattggagag aaaaaggcgc caattcttga gccgtcgctg
     2341 gaatgttcag gctgccaata ttatgaaaga tgttatatca aaagacacga aggcaaacaa
     2401 ttttcattat cagaaatgtt tagtatcaaa aagtaaacta ggtaaaaaaa gtaaaaaagc
     2461 taaagagggc tgtttactcg agtgcttcag gatgaccttt gccgcgtagt gcaaataaga
     2521 ctactgccaa tgcaatggaa accccaattg ctgagccaaa tgctgctaat gatgcttctg
     2581 ccatttgctt aaaatctgcc cccagatctt atataacttt tctaaatttt ttttcatata
     2641 gacaaattat cataatttta ttattttatg atacacttgt cttttccttc aactcacaga
     2701 atgccagatc aagctcctca agttcatcgt tgaacaactc tacaatttct tttaacaaga
     2761 gcttttgctg ctgcgcttcc ctactttctc ctctgattct gtgaattatt ttttcaagtt
     2821 gcggtatttt ctcatttaac gtgttggtaa gctcaagtaa aaacgactcg tagtgtttta
     2881 gggcatctgc aagtggcgag tcagattttt tcagattatt ggcatcatca ctttctacga
     2941 aatcagttag ccttcccctc aaaagacgca gtttggtttc cgtctgagta agcttttcta
     3001 agttactacc aagcaagact ggtgaattat ctgttcgcag ttccagtctt cctaattcgt
     3061 cacttaagta attggtcatt tttttgacaa tctggccttg tttgtgtgct tcttcttgtt
     3121 gttgctcaac ttcatccttt agcttttgga gatttgttat ctctccattg agagtcttgg
     3181 tgagatcaag taaaaactct gcagcatgaa ttagattatt tgcagaatat gagctaagca
     3241 gcatcttgga tctttggact caaaaaaaga tactaaagtt atgtttgttt aatctaaacg
     3301 atcttaagcc tcaaactcaa atgagataat gttttcttga ccgcttgtca ttgagctgag
     3361 attaaagtat gcttcgcctt tgatggtcca cttgatgtca ttatttgata gtgatgacca
     3421 gaactctggc gcattaccag agttttttat tgtaaatgtc tctccaatta tttgtgggtt
     3481 gttgctaaga attgtaaaat agttagaaga gtcaaccatc ccttcgggtc gctcgccaat
     3541 ttgctcaatc gttattcgtt tgcttccatc atagagttca tacttgatca cctgtaaaat
     3601 gactgatttg tagtttggat ttgttatctt aaagtcaatt tgcagtgttg cttcttgttc
     3661 agacacagaa aggatcgaga gtttttcgag ctgaacctca agtggaagca cttggatggg
     3721 ggaagacgat gttctgacta gacctccttc agatacatcg ttgattatct gagagcctga
     3781 aatggcgata aaaccgatta ttcctgcaag tgccgcagca ataattccaa gcaaaacttt
     3841 agagttcaac aatcaatttt tgaattttga gtgtcataaa tgttggtaat aaaatcctta
     3901 tagagatatt ttgatgtggt aagtgtgcaa tattttcaag cagtacaaat tgggaaggaa
     3961 cgctcaagca aatcacagat gatacttttc aagttaggtg gatttgcaat gcttacactt
     4021 acaacaaaaa agaaagaggg acagtttatt ccagttggcg aagaagacct tgttgcggtc
     4081 atcaaaacaa acgatggtta tgtcacaatt ttggttgatt cggagggata caccaaagct
     4141 caatcaaaac cacttgacaa agatgctgca atggaaattt tcaaaaaagc acgagaatca
     4201 gggattgagg aatttgttgg gaataaagtt gagatctgga cagaacaata ccctaccgta
     4261 caaaatgagc taaagtaaag ctagagtgcg atttccgttc caacaggttg gcctttgatt
     4321 gccttttcta gtgtttggat gtctgccttt atgacgcgag tcttgatttt ggagcgctca
     4381 attaccttta gcgctacaat gtccatgaga tcatagccgc cggcaacaga gtcttcacgt
     4441 acaagaaggc ggcggagatc tttgagctca atcctcttga atttttttgc ttttggattt
     4501 ttgtttggat ccgaatcgta gattccatca acgtctgttg cattcaaaaa ctcagtcgcg
     4561 cctatctttt ctgcaataag agcagctgtg ccatttgtac tttgaccagg atgtagacca
     4621 cctgctataa caattaggcc actgtcaact gcgtgacgta tttcggtcag tgtagttggt
     4681 ggatgtggat acgccttggt ctttaatgca tagatgagga gtcttgcatt tagtctagat
     4741 acttctattc caagctcatc tagggtagat tcgtccgcac cggacgatct tgcatgagta
     4801 atgtagtgcc tcgctatctt gcctccacct gcaataacta caggctggca aatcttgctt
     4861 attttgacta aaaatgttgc atagtctttt agcagttttg tgttattatc catttcaaag
     4921 attctgcctg aaagtttgat tactatacgt ttttttgtca ttatctttta tcaccaagaa
     4981 tcaattctgc tgccatttca actttttttc tatgttgaag ggtagtgtat attctcaaaa
     5041 tattcattga ttccgccata gaagacacca ctgggatttt ggaaaatgga atttcttttg
     5101 cagttgattt tgtagcatat ttggtcatca agataatgga ctttgttttt tccttagaag
     5161 gagttaacgg caaagaagag gttgtagatg tatctacaaa aatttcattt atgtcaacct
     5221 ttgatttttt tgcgattttt tgacggatct tatcttgatc ctttagtttt ttgttagtca
     5281 taatttgttc atagacacat tttagcagtc ggcggttttg atagtcagtt gcaattttct
     5341 ttgcacgcct aagatcttgc gtgtattctg gcaatgaaag gatttgtgag ataacaaatt
     5401 catctgtcag ctttacataa tcatcgagat ttaacgaggt gaagttagcc tcatcatcag
     5461 ctaatctgag tgcttcgagc atcattactt ctgcagatcg cacagttttg tgaaagtaga
     5521 ctgccttgaa catttgatat ctagatagaa tcatggactc aaatgagtaa agagcagatc
     5581 tgtcaagtgc tagcttgttt ttgtagacat caagtgattg aatgattcgt ttgtgatcaa
     5641 tttttgcatg ttcagcacca gtaaagtagc catctcgttg tagataatcc atcatgtcgg
     5701 cactaagtga gccagaaatg atttcattaa caaacctgta tttagaatca ccaaacgcaa
     5761 gtttagttat gaatttttta tcaaaaccag ccgcagatag tatctcgcca atttctgaac
     5821 gtaaaataat tttttttccg atttcttcat gtgatgtttt cttttttgtc tgtaagacct
     5881 cttcaaagag atgtgagaat ggcccatgtc ctatatcgtg tagcaatgct gcaaggcgga
     5941 tttcttggat tttgtctgta ttcaaaaatc ctttttcttt tagtgcttgt gctgcctgac
     6001 ctgcaacgtg cattacacca agtgagtgtt caaaacgtga gtgttgtgca ctgggataag
     6061 tgaaatgcgc tcctgagagt tgtcgtattc ttcgcaatct ttgaaaaatt ggtgagtcga
     6121 tgatttttag ctcagtctca tagactcgaa taaaatcatg aattggatct acgatatcta
     6181 ggaagggttt tgtcataatt ttgcgagttt ttttgagaga atcttcatta ttgattggtg
     6241 aatgtggttt tttgttcctg atgcatcaac tatttgccat ccctgttttt ttgctagttg
     6301 tctgtatgtt tttgagattt tattgagaaa aaatgtattt ttttcaaatt tgtcgcggtt
     6361 tgtttttttg cgggaaaatg attcacgttg tgagacatca agtacaatga ctaaatctgc
     6421 ttttggaaga ccagcatcta gaccttcaag ccattttaat gagaggccgt ttgccacacc
     6481 ataaacaagg tttgactgat agtacctatt catgataaga acagaatttt ctgagtgtgc
     6541 cttttttatt tgatctagtt tttcccatct gtttgcagcc aaaagacaat gaattacttg
     6601 tggtggaaat tttcttttgc catgtaaaaa cctggaaatt tccttaccaa ttggggtagt
     6661 atagtctggg aagctaaaga tctttgtttt tatcttactt tttttgagca cacaagccaa
     6721 aagctcagat tgcgtttttt ttcctgcctg atcagttcct tcaattacta taatcaatag
     6781 ttgtattggg gttagtgtga ttaaaaatcc taccaaaatt tataactaaa atagcgagat
     6841 tacagatgca agcgggaaaa ctaaaccctt tagggttggg atgaaagcga gcttatgata
     6901 aacccaagat caatcaaaat ggtttttagg tagaaattac caaatgatat gattgcagag
     6961 aaccttcaag ttcagattgt atcctaattc catacaaaca aaacaactcc actataccct
     7021 tggtgcgtgc agatgggtct acaacaaaat gatagaaaaa atacgctcag aaggactcca
     7081 cacaaggaac gatctcaact attttctaac cgaactaaaa gaacaggaca gatggctgta
     7141 ttcgtatcac agtaaaatgc ttcagatggt atcaacgcag gctgacggag cccagaaatc
     7201 actggcccag ctacgaaaaa acggccacaa gacaggggat ctgaaatttg caagattctc
     7261 agaatataac acctttgtgt acaaccaatc gggatttgaa atcaaagaca gttttttgca
     7321 cctctccaaa ataggcagaa tcaaaataat acagtatagg gatattccag aaaattcaga
     7381 aatcaagcaa atcaccatat ccaaatcaaa atcaggaaaa tggcacgcat gcgttattgc
     7441 agacattgat gccgttctgc caaaaatctc acttgaaaaa tcagtcggaa ttgacgttgg
     7501 aatcaagaac tttgcatatg attccgatgg ataccaaact ccaaacccac tgaacctgca
     7561 aaaaatgctc agaccattgg caagggccca cagaaagata tcaaggagac agctaggatc
     7621 acaaaacaga aagaaggcag taaaatggta tcaaatagta cacgagcgaa tcaaaaacaa
     7681 aagacgggat tttctgcaca agctgtccac actgtatgca agaaaatacg acgtcgtatt
     7741 cttggaaaga ctgacaaaat taaacatggt aaagaaccac agaatggcca gaagcatcct
     7801 ggattcagga tggggtatat ttggaaacat gctcgactac aagaccctcc taatcgaggt
     7861 tccctcaaaa aacaccaccg taaattgttc aaggtgcggg aatatggtgc caaaatctct
     7921 ggcagtcagg actcatcaat gcgacaggtg tggtcttgtt ctggacagag accacaacgc
     7981 aagtatcaac attctcaaaa agggactgga aatttttgga atcacacatc aaatacagca
     8041 ggaattgcgg gaagttacgc ctgtagagat cacaaagtgg tcaatgaagc aggaagccct
     8101 aagctttaac gaggggtagt tcacacaaaa tgggcataat aaaatacatg gcaaaagaaa
     8161 tgacaaaaga aattggcaac aaatctagag agttttatga atttgtccta ccgccagttg
     8221 acatgtatct tgatgaagat tccctgactg tggtagtaga tcttcctgga tttgacaaaa
     8281 aagacattaa actgaccctg cacagaagca ttctatcaat tagtgcggaa aagcatgatt
     8341 cgcagaagga taatgcggtt tataggcagc ggccaaacat aattgataaa aaaattcttc
     8401 taccaatcaa ggtagaggaa gacaaagaag gcgtcgtctc ggcaaaattt gttcaggggg
     8461 ttttgactgt aaagattcca gcatcacacc aaggaaagca aatatcaata gaataactaa
     8521 tattttctaa aaattgcaaa gatacccatt attattagag aactaatcat tccagagatt
     8581 ccggcagttt cgtttgagga gtacaacaat gtggaaccaa tgaaaatagc tgaagacaaa
     8641 atacttccgg caagtaatgt gttacttttt ggccttgcgt taagcagctg aagtgatctg
     8701 ttttcttcaa gaaatttttt tagctcaggt gcaattgaaa tcgtagcatc aagcgacttt
     8761 gcaaatcttg taaaggaata cttgagctct tctatgtatg catccttgat tagttgttcc
     8821 tcctccaaaa tatttttgag aactttgacg aatctaaaat ctactttgtg tgttttgtat
     8881 attccttcaa tgattgagct cattcttaga tacagtgcaa gattttttgg caatgtgaat
     8941 ggaaacttgg acattgtctt gtttgcaagc tcaagcagtg cgcgaacttc catttcatca
     9001 ggtttttttc catgcatggt gcgaattgat agatctattc cttgctcaat tactgagcgg
     9061 ttaaaatctg gcatgagcat cccaagatca ttcattgcgt ttacggtccg aggtggatct
     9121 ttttctacaa gcgcaagata cagtcgtaca agcctcattc gtgtttcctg gtcaattcgt
     9181 ccaaccattc caaagtcgta aagaattaga gaaccatcgt ctgtaacaga tatatttcca
     9241 gggtgtgggt ctgcatgaaa tatcgagtct ctcaacagca ttgtaaaaaa taccttgtgt
     9301 acatctatga ctattttttg tctgtcaatt ccttttttgt ctaaatcctc aatgtttgtg
     9361 atcttgattc ctggaatgta ttctaatgta aggacgtttt ttgaggtgtg atcgtcatat
     9421 acttgcggaa taatgacatt atcatgtcgc tcaagatttc ttttgatttt ttttaggttt
     9481 gatgattctt t
//
LOCUS       DASWKH010000052        64241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2715853, whole genome shotgun sequence.
ACCESSION   DASWKH010000052 DASWKH010000000
VERSION     DASWKH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 64241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64241
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2715853"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..699)
                     /locus_tag="VGA92_03855"
     CDS             complement(166..699)
                     /locus_tag="VGA92_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX9845580.1"
                     /translation="MPIDENFPQGLKPIGKIMHSDGEHYHFMWGPGRSDAEAFSDEAV
                     IADYKKHGEKQVPLGVSGTMVAVDWDSCVADGACIEACPVQVFQWYRTEMDIPAKDVI
                     GKTFAGTGSAVKEERKDKTDKADPIREHDCIWCMACVSVCPPLAIKVDQSNLEHHEKA
                     AGTYVKLEGGTNPHAHD"
     gene            844..1509
                     /locus_tag="VGA92_03860"
     CDS             844..1509
                     /locus_tag="VGA92_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HEX9845581.1"
                     /translation="MEIYKYDVYRGPNIGIYTKVNDSFVFVPNGFAHTKATTLATYLK
                     VEYLFTSVASTRLIGTMMAVNNHGMLLPSTCSEYEVQYLKKSTGLNVGILDTKHNALG
                     NLMCVNDKGGIISPKIPKEVLKNIQDILGIEVIQKKIAGYHQAGAMICANNIGGIIHP
                     ETDDDDIKSVSDVLKVKLEPATINGGIPYVSSGILANNKAVVVGNLTNGPEIMMLTRA
                     FTN"
     gene            complement(1499..2773)
                     /gene="hisS"
                     /locus_tag="VGA92_03865"
     CDS             complement(1499..2773)
                     /gene="hisS"
                     /locus_tag="VGA92_03865"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9845582.1"
                     /translation="MNLPRGMKDFESNEQLKIEHIRQKFLETCKTFGFNFMDPSPIEL
                     VSVIEAKSGPTIRDEIYFFKDKGEREVALRFDFTIGLTRYVVSQKSLKLPAKIAAFGG
                     VWRYDEPQKGRYRYFHQWDLEIYGKPSLESDAEIIEFTSKFFSKLNLKNIIIDISHRK
                     LVEAVVAKVFESNDPQLIGDIFRAIDKIQKKKKDEILQEYQQKGYSKEKLEKILEFSK
                     IKGQPQEVEKAFEVSGMDAWVELRELFSTLKNRGVENIRVNFGIVRGLDYYSGVVFEA
                     FDSSSDVGALVGGGRYDSLPKAFGRDDLGATGVAGGVERIILSLESHGLQNMDSNPQI
                     SVLFVNEEMLKPAINIASKLRNMGVHTEIDLIGRPFKKQMENASNSKFVVIVAPKEYS
                     TNQVVVKNMSDGKETLALVDSLFSDVKSLFNL"
     gene            2863..3279
                     /locus_tag="VGA92_03870"
     CDS             2863..3279
                     /locus_tag="VGA92_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX9845583.1"
                     /translation="MSYKYLEHATDAFIEVNASNLEEAFTLAAKSIIETILDIDAVEA
                     KKERQITVTGKDLNYLLYNWLEELIILTITEGFASKTVKLKIEKNAEYRILATLAGEE
                     IDCKKHHFKMEIKSPTFHLMEIKQNGDVLMRYLLDL"
     gene            3311..3715
                     /gene="trxA"
                     /locus_tag="VGA92_03875"
     CDS             3311..3715
                     /gene="trxA"
                     /locus_tag="VGA92_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9845584.1"
                     /translation="MEDPEIEKIKQKKLEELLKQQQEAIAQSQVEVIDLDSANFDQVI
                     STQSLVLVDFWAEWCGPCKLMHPVFDRMAKKYRHIKFARVNVDKCQDVSMRFGVQAIP
                     TFIMFKNGQPADRMMGAVGEPGIHMIARKYQM"
     gene            complement(3706..5490)
                     /gene="infB"
                     /locus_tag="VGA92_03880"
     CDS             complement(3706..5490)
                     /gene="infB"
                     /locus_tag="VGA92_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX9845585.1"
                     /translation="MRIRQPIVAVLGHVDSGKTSLLDKIRGTGVQSREAGGITQHIGA
                     SFLPTETIKKMCGQLYERLTKSEYEVPGLLVIDTPGHEVFTNLRTRGGSAADIAILVV
                     DTNRGFQPQTTESLKILQARKVPFVIALNKVDQIPGWKKIDTPYITQAIKQQDQFIQT
                     TLDEQIYNVVGTLSILGFQSEAFYRVQDFAKEVAIVPVSARVGVGIPELLTVLVGLTQ
                     QYMQKRLTQEEKPSRGIILEVNDEVGLGTTANMILIDGQIKKGDSVVVAKRDTVIVTK
                     PKAMLLPKPLDEMRDPRDKFKPVDHVEAAAGIKIASPDLDGVLPGSTVYVTNDDGKLE
                     EFKKIIESEMKSVFIDTQTNGIILKCDTIGSLEALTEMLRRQQIPIAKADIGHVSHRD
                     VMDAKIIKDKDRHLGVILAFNVRTLPDAQEESEINHVKIFNDKIIYSLIDNYVQWVDE
                     DTANEEGAIFSELTPVCKFTFLKGYIFRNSNPAVFGIRVDVGKVRQKIPIMNKNGKKI
                     GIIHQLQDGKKTIESATHGQEVACSIQDVMIGRHIFEEDVFYSLPRSSEAKQLIEKFT
                     HKLSPEEQTVLNEIVAIQRKIDSAYGYI"
     gene            complement(5497..5898)
                     /gene="ndk"
                     /locus_tag="VGA92_03885"
     CDS             complement(5497..5898)
                     /gene="ndk"
                     /locus_tag="VGA92_03885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9845586.1"
                     /translation="MTEQTLFIIKPDAVERKLVGQIISRFESKGFKILKLKMFTFTKS
                     QAEEFYSVHSTKPFFGELVSFITSGPVVAAIIEGNNAVATTRLLVGATKSYEAVAGSI
                     RGDFGLGISDNIIHASDSKESFEKEARVAFQ"
     gene            complement(5911..6111)
                     /locus_tag="VGA92_03890"
     CDS             complement(5911..6111)
                     /locus_tag="VGA92_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HEX9845587.1"
                     /translation="MSLIVKPCSFCDRPVSKGSGTMLVKNDGTVLWFCSPKCKKNMLV
                     LKRDPRKLKWTKKYQKGGIIKK"
     gene            complement(6119..6334)
                     /locus_tag="VGA92_03895"
     CDS             complement(6119..6334)
                     /locus_tag="VGA92_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HEX9845588.1"
                     /translation="MSTSQTEDVVPSEVIQIVGKTGIAGEITQVRVKILGGRDKGRIL
                     TRNVKGSVRLSDILMLRETEREAKKIK"
     gene            complement(6337..6714)
                     /gene="rpl7ae"
                     /locus_tag="VGA92_03900"
     CDS             complement(6337..6714)
                     /gene="rpl7ae"
                     /locus_tag="VGA92_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HEX9845589.1"
                     /translation="MAKAYYVKFETPADLINPILEAVRVASQSGKVRKGTNEATKAIE
                     RGISKLIVIAEDVEPPEVVAHLPIICEEQNAAYAFVPSKQELGKALGIDVTSAAAAII
                     DAGDANHIVEEVVSSIKKLKGGK"
     gene            complement(6775..8124)
                     /gene="glmM"
                     /locus_tag="VGA92_03905"
     CDS             complement(6775..8124)
                     /gene="glmM"
                     /locus_tag="VGA92_03905"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9845590.1"
                     /translation="MGKLFGTNGIRGVFGEDLTLEFIHDITMAIATFFKKGPILVGYD
                     GRDSSPVIAKVICSTLNSCGISCNIAGLVPTPALEYATKTLRYNGGIMITASHNPPQY
                     NGLKPVARDGVELSRDGEQTIEGIYFGKKWIKYSKKLGKTATESRVLRTYLDGIKSQV
                     NLAKIKSKKFKVVLDLGNGAQAVSAPILCKELGCKIFQINEKIDGRFPGRGSEPTPDN
                     LQKLSKTVRQNKADLGIAFDGDGDRSIFCDNKGEILSGDKSALLLTNFILKRNPKSKV
                     VTCLNSSSVIEAIASKTKSKVIRTKVGSVEVSRRMVPEKALVGFEENGGFMYGKHNQV
                     RDGCMTLALMLDLLAHSGKSLTGELNRIPKSFTTKDKVLCTKQQANKVIAQLKKEHQS
                     YDTTDGIKIIFDDKNWVMIRSSGTEPIARIYAESDSQQRLDGLMSKYLKKVKAILGR"
     gene            8228..8312
                     /locus_tag="VGA92_03910"
     tRNA            8228..8312
                     /locus_tag="VGA92_03910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8264..8266,aa:Leu,seq:tag)
     gene            8366..9208
                     /locus_tag="VGA92_03915"
     CDS             8366..9208
                     /locus_tag="VGA92_03915"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX9845591.1"
                     /translation="MKKIRVGITVIGKDKEGIVAAFTNFIFQKNGNIEKVNQNVIKGL
                     FGMHLEASFSKAFNIKAFDRELQGLGKKLGMEVSVYHEINSQKNIAIFVTKESHCLRT
                     LLDANAKKQLKGRINVIVGTQRTLEPLAKKARIPFILVDEKDQQKAEDKLVEITKRYD
                     VDLVVLARYMRIMTPNFVWRYPHRIINIHPSLLPAFPGAFGYAQAFERGTKIVGVTAH
                     YVTEELDQGPIIFQDSFKVDSSDTLEVIRQKGQKLEADTLLKAVKMHLDGKLEVSWRK
                     VYTK"
     gene            9205..9915
                     /locus_tag="VGA92_03920"
     CDS             9205..9915
                     /locus_tag="VGA92_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX9845592.1"
                     /translation="MNGIKDFSDPNLRRLIKNKRQIALIPVGSLEQHGSHLPISTDSD
                     IVTEVAERVAKKCNLLLLPTITYGVSFEHAPFFNLSITAQALQKLLTDLCISLAQNKI
                     YKIIILNGHHGNHKPLKALEGKISKISGKKVKVFVFSYWHFMKREFDHAGFAETSLML
                     AISSKVDMKLAKRGLIADRLSPKQRAKISKIASAHFIKATKNGVWGDPTKAAKKDGDR
                     ILSEIVRNITETVSKLAY"
     gene            9986..10216
                     /locus_tag="VGA92_03925"
     CDS             9986..10216
                     /locus_tag="VGA92_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LSm family protein"
                     /protein_id="HEX9845593.1"
                     /translation="MSVDMAVKVLDESIDKIVLIKLKGGKTIRGNLRGFDQHMNLLLD
                     SSEEIPSEGETKSIGTIVVRGDNVVMISPPPA"
     gene            10223..10390
                     /locus_tag="VGA92_03930"
     CDS             10223..10390
                     /locus_tag="VGA92_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HEX9845594.1"
                     /translation="MTRGTPSMGLFTRKRTHIRCRRCGKNSYHKRSHRCSSCGFPDAR
                     IRKYSWIKWYA"
     gene            10391..11362
                     /locus_tag="VGA92_03935"
     CDS             10391..11362
                     /locus_tag="VGA92_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845595.1"
                     /translation="MEVALIISSIAGAATAATINKFPKKNKVSLIGANSHIKAQIDTL
                     RIEKEILTKTISRLYTDSPDLTKIQRDKLLLRYQHQLGVILAKIEKLEQASKHPDLGP
                     LGDGLITLMDQKLSALDKKLVEISTKISVAQTQLEKPEIKKEEPKKEAKIIVEKPLER
                     PEIESTQSIEEIIQSFAKPSKRLEITTLTEIPSNVSVAPPIETKPTIQPQAQTVAQPQ
                     PQIQQVEIVQITQPEPAKQEIIEIIQPQIKQQPVITEPIDIEKQVKSEPPKLEFRNAE
                     PSQEQEKKKSKINIPEPEEIEDDEDDLDSIKREIMKTLSKLEQAEVE"
     gene            11363..12040
                     /gene="rpiA"
                     /locus_tag="VGA92_03940"
     CDS             11363..12040
                     /gene="rpiA"
                     /locus_tag="VGA92_03940"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX9845596.1"
                     /translation="MSFDDAISALSKDALKFVKDNTVLGLGSGRAATAFVKALGQHIK
                     ERKLTIKAVPTSLQIKLVAEQSGIHVIDAEQVQKIDVVFDGADQIDSQKNLIKGGGGA
                     LLRENILISSAKHVVIMADSTKFVKYFNRTVPVEVHPFARSTVKKFIEKLGGKPNLRT
                     IDRGYPFITENGNIIYDCDFGTIKTPKPLAQKIKQIPGVLEVGIFTRKPDVIYKAKEN
                     GKFEILA"
     gene            complement(12037..13422)
                     /locus_tag="VGA92_03945"
     CDS             complement(12037..13422)
                     /locus_tag="VGA92_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9845597.1"
                     /translation="MTVDSVIVEPHVMTPKGVLQKNIVIDEGKIIDFTSDIPQCDNKI
                     SAQGLVAIPGLIDTHVHYGVYSPIEESAKTESRAAALGGVTTMMRMLRLGRSYKTHLL
                     AQLAASGTSHYVDYAIHATIFDEKQADEMKFCTENGITSFKLYMNLGGEVGHVYMDMD
                     PGATRLFETQVQLETSTIQKVLSNAAKLGCPVLVHAEDYQMCECGIKKAKQDKQDGLS
                     AWSKSRPPDSEVKSIKKISEIARQFGTTMYFVHIGSAAAIAAIQEERKKGTKIFVETC
                     PHYLTLSYEKQKGYLAKVMPPIRSSADIDQIWNAILQKHIDTIGTDHVANRLKLKVGE
                     DVWSSLAGFPGIGTALPILLSEGVNKNRINLGQLVELTSLNAAKIFGMYPQKGAIQKG
                     SDADITLIDLKKEKKVDADFFGAFSDYLVYDGWNLKGWPQTTMVRGKLVAENMQVVGK
                     PGHGKLVKRTL"
     gene            complement(13466..14704)
                     /locus_tag="VGA92_03950"
     CDS             complement(13466..14704)
                     /locus_tag="VGA92_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9845598.1"
                     /translation="MSKIKDPSLADRGQFEYEWAKNHMQILTNSIKKLEKSRPLRGIR
                     LGFCLQITKETAVLLVGAKQLGAEVVACGGNPLTTQDDVAAFLDTRGINVYAWSGQSK
                     SEYEWCQNQVLSQKPQILTDDGGDLNIKAHFDKKFKNLKIFGATEETTTGVSRYLAMQ
                     QKKKLRYPIIAVNNARTKMMFDNRYGTGQSTIDALLKTMNLLFASKRVVVCGYGWLGK
                     GVTQRCAGMGSKVIVTEIDPIKALEAHMDGFDVLPIKEASRLGDIFITCTGMTDVIRK
                     ENLLAMKNGAIVGNVGHFDVEVDTKFLLNNSKSVKQVRPNLDECILPNGKKIYLISKG
                     RVANLIASEGHPPEVMALSFSNQLHSILYILKNHKKMKNRVYGVPKEIDDQVALDALN
                     SMNIKIDRLTKKQLAYQQSW"
     gene            14790..14861
                     /locus_tag="VGA92_03955"
     tRNA            14790..14861
                     /locus_tag="VGA92_03955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14822..14824,aa:Ala,seq:cgc)
     gene            complement(14877..15806)
                     /locus_tag="VGA92_03960"
     CDS             complement(14877..15806)
                     /locus_tag="VGA92_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845599.1"
                     /translation="MTAKSGKLFVLIVGIAIVAGIAAAVALVSVTVPNLDAESNGNSR
                     SVFWRHIHGLGIDPTDRRILYIATHGDFYQSVNGGPPVKVDEQRADYMAFNAPPNTGM
                     PLYASGHPSTGGNMGLVKSTDGGKTWQTVTTILEPPVDFHAMAVTKSNPNMIIGFDSA
                     GRGLFKTVDAGLNWQKLDPPDYISALAISPTDPDVIFAGTGKGIYQSNDGAGTWIQLE
                     QYKGLLVFALIFDENGTLYASTDQFGLSRSTDSGQSWNKVSTTNLTIMSITTDVQNKI
                     LYVAGYSPDGFQEVYRSLDDGNSWELIGTNKEL"
     gene            complement(15859..16332)
                     /locus_tag="VGA92_03965"
     CDS             complement(15859..16332)
                     /locus_tag="VGA92_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX9845600.1"
                     /translation="MNALIISIVIIGGILISASYLLSQTLAEEKLHSSSMMNMCGMMM
                     NKPPKDVTISPASSQLVQAGKEAKITILVKDKTTKQPLLDGIVSIMIERGPSMSTMEM
                     VTPMFAAKEIGDGKYQITFIPDKKGVYTIHTHVIPPTKSMHSMMNNHMDIGVIAK"
     gene            complement(16329..18455)
                     /locus_tag="VGA92_03970"
     CDS             complement(16329..18455)
                     /locus_tag="VGA92_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9845601.1"
                     /translation="MAKDPICGMFVEEKDNSIKHAVDGRVYYFCATSCLNEFTQPEKE
                     LKKLKTHVAISMALTIPIILLTLPHMLEQFPSFLPMHISNYVMFALATPIQFWIGWRF
                     YRGLWDGLKAKASNMDTLIALGTTAAYLYSTIVTFVHGFFPVGSVYFETAAIIITLIL
                     VGRLLESKTKEKASSAVRKLLDLQPRKAKILRGNTEVEIPTEEVQEGDILVVRPGERI
                     PTDGVIIDGLSSLDEAAITGESIPVDKSVGDQVIGATINKNGLLKITATKVGQDTVLS
                     QIIVLVEEAKTGKAKMQKMVDQVSKYFVPAVIAIAILTGLGWYFVGEVGFDYALLAFV
                     SVIIIACPCALGIATPAAMMMGSGKGAENGILFKGGEYLEVAKKVNTVVFDKTGTVTE
                     GKPSVTDIIDFAEMGKTELLRLAAIAEAGSEHPLAQAVVKKAKEEGLAVSNPDSFEAI
                     MGHGLKATYANHKILIGNRKLMEQNNIYVVEKTEATMQQLEADGKTVTLVSIDDKLAG
                     MIAISDTIKETAKEAIDALKQNGIEVIMLTGDNERTANAMASMLGFEKVIAQVLPHQK
                     EQVISKLKSEGKVVAMVGDGINDAPALASADLGIAIGSGTDVAKETGGIILIKNDMRD
                     VFIALELGKKTVSKIKQNLFWSFAYNTGLIPVAAGIFVPVLGLGMFSWLPIFAGAAMA
                     ISSVTVVGNSILLGRYRPKFIKTDVI"
     gene            18831..19268
                     /locus_tag="VGA92_03975"
     CDS             18831..19268
                     /locus_tag="VGA92_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEX9845602.1"
                     /translation="MPFQLDDVDIAILESLIKDGRKSFRQISRETKVSTPTVQARYER
                     LVNVGLIKTVSPILDLGKLEDKTGERLKNIRTKAIKHHDVKITKNMMIKMTCDYCEGP
                     ISEKPNVLKIANFERFFCCTSCRGLYKEKYRGRIESLSRKQII"
     gene            19308..19670
                     /locus_tag="VGA92_03980"
     CDS             19308..19670
                     /locus_tag="VGA92_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX9845603.1"
                     /translation="MKVFNAKAAAQDYMSAHTLAFSTPELTLMRYSFWLGDMIPDPKD
                     KSNTIPRMLSYVEEKDFAPVEIIDDEHYEPTGAVRNTGMYGNINKATDANTKFCSECG
                     AKISLNAKFCTECGTTQD"
     gene            complement(19667..20191)
                     /locus_tag="VGA92_03985"
     CDS             complement(19667..20191)
                     /locus_tag="VGA92_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX9845604.1"
                     /translation="MSLGEIDTLNLLAEKLDNLFQDSQGYYESFLDANTLYKQGKLSE
                     KEFFEKLGDYVVAYSALEFLAVKVIFEMKKALDRVGAGNLGTTQSPGLMPGMGSMGGM
                     PGMAGRVPMTPAQTPGMPPSIVSAREGFGSPGTLPTTDSAMMPRSTGSSSCKSCGSEL
                     RVGAKFCTKCGARA"
     gene            complement(20246..20578)
                     /gene="trxA"
                     /locus_tag="VGA92_03990"
     CDS             complement(20246..20578)
                     /gene="trxA"
                     /locus_tag="VGA92_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9845605.1"
                     /translation="MSLTHVSNAKDWQSQVIDSSKPVFVDFWAEWCGPCRMVGPVVEE
                     LAKDYDGKVLFVKVNVDEANELAAKYNIFSIPTLMILNKGQVVAQQVGAASKEAYKNM
                     IERALSKA"
     gene            20680..21450
                     /locus_tag="VGA92_03995"
     CDS             20680..21450
                     /locus_tag="VGA92_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845606.1"
                     /translation="MSSELRVKKLRGSGGYVMAQVSDEQQRKGNLGGPDLFLAPIGRL
                     EPQIISKYYCNTCEKDYEGSPKIEYENPNEEVAQNLILLERGQYLCTICGSTIAEYRE
                     FQKPNQQADVGLAKPITQTFEQPAVFVPTVQPTIEPVIEPPIELEAPTFSSTTDTPSS
                     FSSIVGMLVYDSDAKRMGVAKQVGVDSAHQVVLIVTKNNGQDETIEWGRIKKIGEIIL
                     LGEEGQSASFSSQGLRCTKCSFENKSGSKFCESCGNKL"
     gene            21452..22153
                     /locus_tag="VGA92_04000"
     CDS             21452..22153
                     /locus_tag="VGA92_04000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9845607.1"
                     /translation="MAKSTAKSIVKDIIIVAVGVAVIWFGLQVIFGTPNPFYVVSSGS
                     MIPVLEVYDVLVVDGKVPFNDIQTGDIIVFNRPNGHDRVIVHRVVAVLDEEPKTIRTK
                     GDANPVSIPGTDYPITKDEYIGKVAYVIPQIGYVTRILTPPINYIIIAVIIGIMIVKQ
                     YSKGKKHEPKFDTEPQKDPLTDSEEFPKQDDIVYSDPNSDITKKEEPKPDSNVESDVT
                     LDSNKKDESDQNKSQ"
     gene            complement(22165..23670)
                     /locus_tag="VGA92_04005"
     CDS             complement(22165..23670)
                     /locus_tag="VGA92_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9845608.1"
                     /translation="MTIRTGQEYIQSLRGRNLIVYLFGELVKEPVDHPMIRPSINAVA
                     ETYDLAAREEDLAAPVSKISGKRVNRFLHIAESATDLVLQNKMQRKLGQLTGTCFQRC
                     VGMDALNSLHSVTFEIDEKYHTSYHKRLLEFIKKMQEENLVIGGAMTDVKGDRGKAPF
                     EQADPDMFVHIVKRTDEGVYVTGAKAHQTGCINSHWIIIMPTIRLTDKDKDYAIVGAI
                     PVDAPGITYIYGRQSCDTRSMEGGDIDAGNAQFSGQEAMIILDNVFIPNDKIFTNGES
                     EFAGMLIERFTCYHRRSYVCKTGLGDVMIGAAASIADYNGVPDVSHIREKLVEMTHLN
                     ESIYAAGIASSYQAHKMKSGVWINDDMLANVCKHNVTRFPYEISRLAQDIAGGLMVTM
                     PSEKDFKNPVTGPLLKKYLAGRKGVDVENRMRILRLIENMTLGRNAVGYLTESMHGAG
                     SPQAQRIQIARQMQLGYKKNLAKNLAGVKGDAEESPENSDYFKRVFKIPEK"
     gene            complement(23774..24481)
                     /locus_tag="VGA92_04010"
     CDS             complement(23774..24481)
                     /locus_tag="VGA92_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX9845609.1"
                     /translation="MQNASIRKLSANKKIFFAELVGTFVLVVCATGSVVLNVKSAGTI
                     GLWFEAFAPFVAVAVMATLFAKTSMAHFNPAVTLTFLITKHMPAKLLPLYLGAELIGA
                     FLASLFVKFVIGTEANLGANFPNYEFPIYVIIGIEAIATAILISAILVAVHTKGLRGF
                     GIVMFASTIFFDIYFLSFISGASMNPIRSLAPAILSGYTSDLWLYWTAPFVGAVIIAV
                     VYRKFFNTRTKNLISSA"
     gene            complement(24463..24867)
                     /locus_tag="VGA92_04015"
     CDS             complement(24463..24867)
                     /locus_tag="VGA92_04015"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX9845610.1"
                     /translation="MPQNKKILFVCVENAGRSQMAEGFFRKYCPPGYEPQSAGTQPTN
                     EVNPFAVEAMKEIGIDIASQKPKTISDSMIQEAARIVNMGCMDKESCPALFVNDVVDW
                     SIPDPKGKSLDEVRHIRDMIEKKVQELCKTLQ"
     gene            24911..25210
                     /locus_tag="VGA92_04020"
     CDS             24911..25210
                     /locus_tag="VGA92_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9845611.1"
                     /translation="MNGTTLLKCICEDTRFSILELLQKNSEMSVNDIVSKLKKDQPLI
                     SHHLRALKECNIVKTRENGKMTMYSVANKDLSRLISDIIKTSEKIASCCESTTCY"
     gene            complement(25298..25435)
                     /locus_tag="VGA92_04025"
     CDS             complement(25298..25435)
                     /locus_tag="VGA92_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845612.1"
                     /translation="MVQDPKPSHWNYEVRASFKKVIAWDTLFFVLGIGVGAGIGAFLT
                     I"
     gene            25544..25756
                     /locus_tag="VGA92_04030"
     CDS             25544..25756
                     /locus_tag="VGA92_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845613.1"
                     /translation="MIKMRVDLCRKCGNELRKFNQAERCNTCGEEFSQFTCARCDIVT
                     EPQYHVHTDSNTTFENPITYLYKRRN"
     gene            complement(25883..27982)
                     /locus_tag="VGA92_04035"
     CDS             complement(25883..27982)
                     /locus_tag="VGA92_04035"
                     /EC_number="6.2.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate--CoA ligase"
                     /protein_id="HEX9845614.1"
                     /translation="MAESPFLSPKSIAIIGASDKEGSIGRAITSNIMKGFTGKIYPIS
                     PTRDKVFDMPAYKTVLDTKEQIDLAVVITKNDVVPTVLEECGKKGIRGVIVITAGFKE
                     VNEEGRKLEQQLKDIVKKYGIRLIGPNCLGVMNLDPNTMMNSTFLKITPKSGQIALVS
                     QSGAICAALVEDASAQGIGFSAVVSLGNKADMSEIDVLKMLAEHEQTKVIVMYLEDMG
                     DGQEFLRVCKQVTKKLKKPVLVLKSGRSPEGARAAMSHTGALMGSDEIYDAVLHQAGA
                     IRVDTMEELFDYATAFSKQPLPMEGDLVIVSNAGGPAIISTDACSKFGIKMAKIEDIR
                     PKIDAVIPPWGSSRNPVDIVGDADFNRFNNVLENVLAHKNVGSVIVMCTPSATLDYDK
                     LAEVIVKMSKKYKKTMLASLMGLDEGIKNREILAAGDVPYYTYAEGAIRTLRAMLRFT
                     NWLKTPEGIIAEFQVDKSKVKNVFDAVKKEGRTNLLEEEGQEVLRAYGFPLPQSILAT
                     TESEAVNTAKKIGFPVVMKIASPQIIHKSDASGVKVGVSSEQGVRDAFNQIITNAKKY
                     NKDATIKGILVQEMVKGGKELIIGSKLEPGFGPVIMLGMGGIYVEVLKDVTFRLAPVT
                     DKEADDMINSIKTKKLLEGVRGEKPSDLKKLSELIQKLSALVTDFKEIKELDMNPVLV
                     MEQGNGCKILDVRIGLS"
     gene            complement(28043..28399)
                     /locus_tag="VGA92_04040"
     CDS             complement(28043..28399)
                     /locus_tag="VGA92_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEX9845615.1"
                     /translation="MALSVYVDGSGGDNAGYGYFVKETGESFYEKKQNITNNQAEYLA
                     IIAAIQKLGQSSDQITIYSDSKNTVSQLNHEFAINSEQLRELARQAWSLLGKFPNIKI
                     VWIPRAQNLAGKMLGS"
     gene            complement(28389..28841)
                     /locus_tag="VGA92_04045"
     CDS             complement(28389..28841)
                     /locus_tag="VGA92_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845616.1"
                     /translation="MQKRYREYLKLNRSLILSTIVAVLLSAITAQLFADHESYINSSY
                     TVTVDMTTFYSVFITLFYFDNRKKYRLESGKLDSPRLKKDLVKIVSSLGVGELVYVTT
                     RWSLQYYFLTINYEAYLASIAAHVVSIVLYLIVVNVGVRITRLYKDGT"
     gene            complement(28877..29161)
                     /locus_tag="VGA92_04050"
     CDS             complement(28877..29161)
                     /locus_tag="VGA92_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845617.1"
                     /translation="MESTSAVLSGVSIANMIVLGILMFVFGRMYANTKAQLPLGMIVF
                     SVMLFLHNTIGAYAYFSMSQLFSHEIFPYLLGVHIAELAGILIFLKITMD"
     gene            complement(29188..29490)
                     /locus_tag="VGA92_04055"
     CDS             complement(29188..29490)
                     /locus_tag="VGA92_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9845618.1"
                     /translation="MANDPYAKRLLWFVFAGSRGGLNRLRLVLAIKEKPLNANQLAKE
                     LGLDYKAIQHHIGVLEKNNIITRLGEKYNVTFFISNFLEVNMDSFNEIIDKLEKSK"
     gene            29699..30472
                     /locus_tag="VGA92_04060"
     CDS             29699..30472
                     /locus_tag="VGA92_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9845619.1"
                     /translation="MYRSKEYVERSWDEIKAEIDIMAKSLPQTKRIFLADGDALNLAT
                     DYMQKIVKYLYEKFPNLERVSCYAMPMNVLKKTHEELKTLREAGLNMFYLGIESGSDI
                     ILKKVTKGATAATIIRACKKAMETGYTLSCMIILGLGGKTYSKEHIKGSAEVINASSP
                     HYVGALTLYLENGIKEEFLTKFGEPFIPVNDSEALDELEDLVGQIDVKNQVIFRANHG
                     SNAFTIKGTFPQDKQMMLERISWMKQHPETARPQGLRGF"
     gene            complement(30510..30761)
                     /locus_tag="VGA92_04065"
     CDS             complement(30510..30761)
                     /locus_tag="VGA92_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845620.1"
                     /translation="MTDLSFNDKLILVQYAIQKYENEATLLEKLKTVLPEKDAQRSID
                     TLIGTQRVRRIGPEILQNNESHTELPVLPDHLKPIIDGL"
     gene            complement(30796..31722)
                     /locus_tag="VGA92_04070"
     CDS             complement(30796..31722)
                     /locus_tag="VGA92_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX9845621.1"
                     /translation="MTDLDFCTPNDVPPEFLALNQDVEMLGVGTPGKTGILRIVLEKD
                     PTKNRTILTEQYSKVPLYTQKVLHYDSANPAMAYLFIMSSSGGIIQGDRYRMDIALKN
                     NALANITTQGATRIYKMDSNYATQLLNIDVAENCYLEFIPDQIIPYKNSRYYQKVNIT
                     AHDSATVVYSEVVSSGRAAMGEMFSYDICYLRTIGKNQKGEMKFTDASMLNPQKQKFS
                     SLGMLEGHTIFGTIYILTQKHVQNIQEKISNLFDNNNEVIGGCSFLPDNSGLVVRIVG
                     NYFDDIKTTIYEAIRIVRKEILNSTFEGIRKN"
     gene            complement(31719..32375)
                     /gene="ureG"
                     /locus_tag="VGA92_04075"
     CDS             complement(31719..32375)
                     /gene="ureG"
                     /locus_tag="VGA92_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX9845622.1"
                     /translation="MSDRISRVGIGGPVGSGKSTLIEQVVPVLAKQGYRICIISNDVI
                     SKEDAERMRKNLATQQKLLPENLVIGLATGGCPHTAIREDPSMNLALIDEIERDHKEL
                     DLIIIESGGDNVTTTFSPILADYFIYIIDVAGGDKYPRKGGLGIESSDLLVINKIDLA
                     SQVGADLSMMEHDAKKMRKEKPFVFVNCKNDTGIKEIAEHIVRDVLFEQPPKALLHQR
                     "
     gene            complement(32396..33091)
                     /locus_tag="VGA92_04080"
     CDS             complement(32396..33091)
                     /locus_tag="VGA92_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX9845623.1"
                     /translation="MSMDDFADLGLMQLADSFFPSGLYTMSNGLESLYLQKRITSPEQ
                     VQNFVEVYFLQQIGPADCVALANAYDFVENNDTNALFGVDESIYKIKLIKEQRDASVR
                     SGRQLVKCLASFLRDDLLDKYLQAISSSKTPGTYPVSIAIAAKLFGIPKQKAALMLFY
                     GFAVSVIGAALRLGILQHFEGQKILNELKPTISETVQKYLKVPYEQMWQFAPQLDIIQ
                     IHHEKMDSKMFIT"
     gene            complement(33078..33575)
                     /locus_tag="VGA92_04085"
     CDS             complement(33078..33575)
                     /locus_tag="VGA92_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Urease accessory protein"
                     /protein_id="HEX9845624.1"
                     /translation="MLLSNSIYGNIFRDSKLAKEFEAQSQNYKRLVLSRHEMEKTRLR
                     KKTQDGTDIGIDLASGIRLHHGDVLSIDSIKILVEQLPEKVIRVNSKEEKNSELFVML
                     GHIIGNRHRPIAYEDGSIIFPIYSESEFDLFKSLFKDMTAKIELSVEEKVFQPHDTMD
                     VHEHG"
     gene            complement(33618..35309)
                     /gene="thsA"
                     /locus_tag="VGA92_04090"
     CDS             complement(33618..35309)
                     /gene="thsA"
                     /locus_tag="VGA92_04090"
                     /inference="COORDINATES: protein motif:HMM:NF041082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HEX9845625.1"
                     /translation="MANLEKIIPEDLRYTKSDKSRRYNLLGARLTADLVKTSLGPRGL
                     EKMYIDVLGDATVTKDGAAFLRKVDLFHPAAKAVIEAANSVDNAVGDGTITVTVLIGA
                     LIQKAEELLDLGIPAPVIIQGYERGLEISLETLYNIAKTKNNSDAAVLENLALTCLRS
                     KAISNIADEDTIAKLIVKAVSTISDFKNNKIEHDDIKIEEKPGSADKTTLIRGMVVDK
                     TIDNSQMPKNIQNAKILLLDDELDNQTTKTESEIIITSVGQREVFLQKKSEEIKNKVS
                     MIMDSGANVVFSRKGINLRAQEQLLKAGIASVRRVKENDLHWLSKATGAKIVSLDEVS
                     KDMLGFAGKVYEEFVGDDKMVFVERCINPKAVTILLRSQSKRYLDEFHRSVLDAIHVL
                     VDFIVKPQIVVGGGSTEALISKKIRQVAYTIEGKEQLVVAKFAEALEEIPLTIARNSG
                     MSVTDVYSQLRSKVDQISLENISKWYGIDAIERKVQDLSTKNVIEPMVVKEQILKTAS
                     EVVQMLLRVDDIFEKKPLDNTHTHADGTTHSHAGGSEKHDHDAMGKIQRPMHHYY"
     gene            35538..35792
                     /locus_tag="VGA92_04095"
     CDS             35538..35792
                     /locus_tag="VGA92_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845626.1"
                     /translation="MGSVTTKDDIRRESERVIKALYGNGVADLAIREVFALPEKGPRT
                     AWDVQVTFMLEKLKYTVDLEMQEKNGQVTNARLIDTMVPL"
     gene            complement(35827..36348)
                     /locus_tag="VGA92_04100"
     CDS             complement(35827..36348)
                     /locus_tag="VGA92_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845627.1"
                     /translation="MPGVGYGTIGLKPDVLTRLKKITGDHYPGMFLPSSLIILMNEIK
                     LGYYTVEPHNIKIDLNGKYTSITIRTDVKVWLENNYKILKDEYKEKYKIENFTQFLSY
                     FLVNMFESKRDSQKYVIRINESEFNWLQSEYKKRKKEYAQKYDAMTFERFADVYLKQL
                     LEKIQEAKKILTV"
     gene            complement(36404..36571)
                     /locus_tag="VGA92_04105"
     CDS             complement(36404..36571)
                     /locus_tag="VGA92_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845628.1"
                     /translation="MDKKDIFATIIILAGLGLTQLEGGTSPFYVGLGIGTIIMASVWI
                     IFRIIKEYKKK"
     gene            complement(36591..36992)
                     /locus_tag="VGA92_04110"
     CDS             complement(36591..36992)
                     /locus_tag="VGA92_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9845629.1"
                     /translation="MKKILVAIGDESSLNRCLNIAIPIAKGLGASITGIHVLAPYPRS
                     FVTALEQPWRKQQKENAEKYLDMAKKKCEEHGVTFSKIISRGQPREAILEHEKEFDLL
                     VLGRADWGSKLLGSVSNGVVTKATREVLLVH"
     gene            37201..38769
                     /locus_tag="VGA92_04115"
     CDS             37201..38769
                     /locus_tag="VGA92_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX9845630.1"
                     /translation="MGEITVVGWAIIGFLIVTIVVGTMTYRLVQRSGRRLMIAGKSLP
                     LFMVGTMLAAQSIDGNSSLGNVALVYDFGFWAGAVIPIGLGICLLMTGLFYGKILNKM
                     SMFTLPDFYFRRYGNGAEGISGVLMIISFTVLVAGNFAASGFILETVFGISYLWAILI
                     AAVIVLAYTFAGGLFSSAYTDLFQIYLAIGAFWAGFLFFAGGFSGTEFSTILDATPAG
                     YLDLSGLYDVANGALLNWAAILALGLGDIVALDFMERVFAAKTPKTAQRGAFMGAALT
                     FFTVVPTSMLGIIAAFYLPGLEDPNLALPELALNHMPIAIGAAILMGVLGASMSTANG
                     GLLAISSVVSRNLIQRIIRRRWLGREAWTDKKLLWITRAITIPMMVAGLVLGYFVPAP
                     GIYLILAFDIVFAGAFAPLTLGLFWKKANMPAAIASLLFGSGLRLFFFFAFDQGLIDA
                     EWAGLDTLIPPPISFALFIGVAYATQKRYPGKARHDVRDYVPPEEDLISGEDLKHFKE
                     VGDAGGNISDRRAI"
     gene            38798..40018
                     /locus_tag="VGA92_04120"
     CDS             38798..40018
                     /locus_tag="VGA92_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9845631.1"
                     /translation="MKNCWHHINRGLVSLSYDNYTKAVSEFDKAIKIESDNISAHLHK
                     GIVLYKMHKYEDAIGCFNKVIEKDPKDIAALYHLGRCLEGIGKYESAISCFEKILKIE
                     SQYEEALIYKGLNISNLGRHVESIFCFDEILKKDPHNSSALAKKGLVLNRIDKKDEAL
                     FYLNKSAEEDSKNSLKMFKNGFELTREGKHEEALKHFNKALELDPKNVKALVQKGIIF
                     DIAGFSRKEIVAFFDRALEVDPKCIDALLNKGFILDRTGFFEKALKCFDKVLEIDPVN
                     IRATYSKGISLFSLKKNAEAMFYCKRAIELEPSNLKALGSMVWLLEHMKKYEEAVEYC
                     DKAIMLEPNYVYALNYKGKILYNLGKYVEALKYYDEVLRIDPINHRAIYYKNDIIAKT
                     SKKKRFGFIAKLKN"
     gene            40220..41335
                     /locus_tag="VGA92_04125"
     CDS             40220..41335
                     /locus_tag="VGA92_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020221833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEX9845632.1"
                     /translation="MGMEFYGESYRGNDEPIFVGIPTFLKLPWLRSKAELEKVKPDVA
                     IIGEPFDFGTTIRPGARYGPRAIRAASTVPSPPYEHFNIETGVDPFGVFRTIDYGDVN
                     VSPGDVVESHRRMTDKVKEVLDIDGIPVMLGGDHSITFANVRAFAKKYKNIGMIHIDT
                     HADCAPQGLCGFKYDHGAHIRRIMELGCLKGKNYTLIGPRGYWPGPDLYKWMADQDFQ
                     WFTMLDVEEIGIDKIAKEAADRANDNVDAVYISWDIDTFDPAYAPGTGEPEPNGLTSR
                     EGMKLMRLLSTSFDPDKFAFDLVEVAPNYDVSDGNSYNGGITSGLANRLIVELLAGLS
                     LTKKGLKEGKPVRPNFYRGTGHTYSFGKEKAEIPKRD"
     gene            41332..41721
                     /locus_tag="VGA92_04130"
     CDS             41332..41721
                     /locus_tag="VGA92_04130"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9845633.1"
                     /translation="MIHVKVTVKGEPDTSPFTRVFSYPKKTDEEIFMNSASMIKERLD
                     RNLKININEALIVYSSYIVLELGENKSVEQIQKNAHRILSSDHVLIGVPETLREMSFE
                     VKLDDRPIQVVILNTPIPVSDYVLKSI"
     gene            41741..42505
                     /locus_tag="VGA92_04135"
     CDS             41741..42505
                     /locus_tag="VGA92_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HEX9845634.1"
                     /translation="MIENLIEQIPGATPVFVLVAGFVIGLAHAFEPDHVAAVATQATK
                     SRQNRSLPVFRRIGVGAFKGSILGALWGAGHTSTLLLVSLLIFVFSLSIPPQVFSNLE
                     LIVGMMLVFLGASIYLNKKLLRKRHEHPHVHEDGMIHTHQHDHNKEHRHGHKSYIIGC
                     IHGLAGSGALVVLALSTLSDVQIILSFVLVFGVGSILGMMMVSGAIGLPFVISTHSEK
                     IHKTLRFVVGAISITIGFDILYNVTLESGNFFGLHL"
     gene            42580..42882
                     /locus_tag="VGA92_04140"
     CDS             42580..42882
                     /locus_tag="VGA92_04140"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9845635.1"
                     /translation="MFLTPKDIDKLQIFLAAELARRRKARGLKLNHPEAVALIADHIL
                     EGARDGKSVADLMSSGKKVLTKDDVLPGVVDLIHTIQVEATFDDGTKLVTVHDPIV"
     gene            42888..43250
                     /locus_tag="VGA92_04145"
     CDS             42888..43250
                     /locus_tag="VGA92_04145"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX9845636.1"
                     /translation="MIPGEYLISDKPIIANQGKKTVKIRVANTGDRPIQVGSHTHFFE
                     ANKALEFPRAEAYGFHLNIPSGTSVRFEPGDSKDVELTEYGGKKIVWGFSGLVNGPLD
                     EKRKEALERAKKEGFRGA"
     gene            43247..44959
                     /gene="ureC"
                     /locus_tag="VGA92_04150"
     CDS             43247..44959
                     /gene="ureC"
                     /locus_tag="VGA92_04150"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX9845637.1"
                     /translation="MTLEISRKRYVDLFGPTVGDKIRLADTDLIIEVEKDLLRYGDEV
                     VFGGGKSARDGLAQASGVKREQSLDLVITNTIVMDPVLGILKADIGIRDGRIVGIGKA
                     GNPNIMDGVDMIISSNTEVISGEHTICTPGAIDSHIHFISPQQIITAICGGTTTMIGG
                     GTGPADGTNATTCTPGKWNIHRMIEAVEDFPLNFGFLGKGNDSQETALLDQIREGACG
                     LKLHEDWGTTPATIDAALRVADKTDTQVAIHTDTLNECGYVDDTIAAIAGRTIHTYHT
                     EGAGGGHAPDIMKIAGEPNVLPSSTNPTRPFTVNTLDEHLDMMMVCHHLNPSVPEDVS
                     FAESRIRAETIAAEDILHDIGAISMMSSDSQAMGRVGEVTIRNWQTADKMKKIVGRLK
                     EETGDNDNVRVKRYLAKITINPAITHGISEYVGSFESAKIADLVIWTPQFFGIKPKMI
                     IKGGFIAYSLMGDPNASIPTTEPVYYRPMFGAFGRAKKSISFVFTSKLAIEDGLENKL
                     KTRKKFLAVQNCRKIGKKDMIHNNITPKIEIDPETYQVTVDGKIATVEPATKLSLTRL
                     YNLF"
     gene            complement(45031..45597)
                     /locus_tag="VGA92_04155"
                     /pseudo
     CDS             complement(45031..45597)
                     /locus_tag="VGA92_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
     gene            complement(46016..46156)
                     /locus_tag="VGA92_04160"
     CDS             complement(46016..46156)
                     /locus_tag="VGA92_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845638.1"
                     /translation="MKTITKPDHWNYQVRAPFKKVMAWDTLFFVLGIGVGAGIGAFLA
                     TA"
     gene            46356..46715
                     /locus_tag="VGA92_04165"
     CDS             46356..46715
                     /locus_tag="VGA92_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845639.1"
                     /translation="MSSDKEFQSELNNFIKTMHDVFSHLLDEYNNKFDLKIEKVGLNN
                     FKAKAKKLSKIDAMNFLIWYEKEYQKIKSDEMCGYLLEKKDDLNFNFSNINNVIAACS
                     MLLEKTRTMTYFAYENF"
     gene            complement(46752..47621)
                     /locus_tag="VGA92_04170"
     CDS             complement(46752..47621)
                     /locus_tag="VGA92_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9845640.1"
                     /translation="MYKIGIDLGGTKIEGLCLDDNLQIIQRKRIPTNQQNGYHSMVES
                     IISLTKDLTKDISNYSVGICTPGTISKKTGLIKNSNTQCLIGMPLKDDLEKSLGKKIT
                     MENDANCFAMAEATTGAAVGYGVVFGVILGTGVGGGIIIDGKIHRGRTYIAGEWGHHT
                     LHQNGNKCYCGRFGCVETYISGPALEKRWSELTGRTKSLTSIVQNLNSDEAQQWKKEF
                     LENFGMGLANVIDILDPDVIVLGGGVSNVDFLYTEGRNAVYGQVFSDLVDTPILKNKL
                     GDSAGVFGAALLI"
     gene            complement(47671..49284)
                     /gene="thsB"
                     /locus_tag="VGA92_04175"
     CDS             complement(47671..49284)
                     /gene="thsB"
                     /locus_tag="VGA92_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX9845641.1"
                     /translation="MASIQQTPNGPVLVLKESALQQKGRDAQKNNIMAAKLVADLVRT
                     SLGPRGLDKMLVDSIGDVTITNDGATILKEIDVQHPAAKMMVEISKTVDNEVGDGTTS
                     SVIFAGALLEKAEELLAKNVHSSVIIEGYQAAQEKAMELLSQIAKKVEPTDHESLLKI
                     ATTSMQSKLISEDSSILAKLVVDAVLRVAEKKGDKYVVDLDNIKVEKKAGASIQDTDL
                     IKGIVLDKEVVHSGMPTKIEQAKIALLNAALEVEKTEMSAEIRITDPTQMQMFLEEEN
                     RMLKSMVDKLQKVGANVLICQKGIDDIAQHYLSKYGILTVRRVKESDMTKLAKATGGR
                     ITTNLDDMTEKDLGHADLVQQRKVESDKWVFIEGCKNPKSVSILIRGGSQRVVDEVDR
                     SLHDALMVVKDVIEKPAIVAGGGAPEAYLASQLKEWSDSFEGREQLAIRKYAEALEVI
                     PLTIAENAGMNPIDTMANLRAKQNSGRKWAGINAKEGKIDDMFLLNIVEPVSVKEQIA
                     KSATEAACMILRIDDVIAVSAGPGGAAHR"
     gene            complement(49328..51046)
                     /locus_tag="VGA92_04180"
     CDS             complement(49328..51046)
                     /locus_tag="VGA92_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9845642.1"
                     /translation="MGEKKANLVLKNCSLVNVYTKEILPNAQVAIVKDRIAYVGSDAS
                     HTVNTSTAVIDLENKYLSPGFADPHMHIDQFVMPAELAKHSLLCGTTSLFSDPIDIVS
                     VCGFRGFREFVKASSGLPIRIFHVVPGGLPVDRKFSHGKTLSLAEQKSAINLDGVVGL
                     GEVFSWTKVTTRDPQTMKKLSNMLQNDCIINGHTAGASDKKLNAYISSGILSCHEPID
                     YDQVMERLRLGMWIMMREGSIRRDLKNIIPQVLATKPYLNRLMFCSDGLDPIDITKYG
                     HIDHCIRESMRLGVDVIDAITMASKNCFDYYNMGKDLGGIAPGKLADMLVFDDLEKIK
                     PKKVFVGGQLVVSNGSIVTKIRKAPLPKWITKTVKLAKPITEKDFVIKSKNPTVLANI
                     IEMETEIITKQNTAELVVKAGNVVSSTDKDIWKVAAFDRTYGSGKHTVGFLKNFGANV
                     GAYASTWCFHENDMIVIGSNEKDMAQATNHLARSQGGLVVVKDGKILSFLPLQVAGII
                     SSNQFDDVLQKFVHLNSTLTDSGCRFQRPHLLPLFLPFLALPSVRILYSGIVDVKKRS
                     FIPSLA"
     gene            51168..51449
                     /locus_tag="VGA92_04185"
     CDS             51168..51449
                     /locus_tag="VGA92_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HEX9845643.1"
                     /translation="MNVPKEINRYCPKCKTHTLQKVSLYKAGKRRGSAIGERRHEEDK
                     KGYGGQKFPKLAKPAKTTKKQTLIFACPQCKKKSNKKGIRLRKLELVAH"
     gene            51446..51643
                     /locus_tag="VGA92_04190"
     CDS             51446..51643
                     /locus_tag="VGA92_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HEX9845644.1"
                     /translation="MKRSHILIPTPKSKFQKVQCKECSAENILFSHVTTIVTCKSCGN
                     VIAEPTGSIAKIYGTVSGVLE"
     gene            complement(51626..52204)
                     /locus_tag="VGA92_04195"
     CDS             complement(51626..52204)
                     /locus_tag="VGA92_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX9845645.1"
                     /translation="MIGLVMAGGKGTRMGLPEEKLLLKYKKPVILHVIDALKESECFS
                     KIIAATSDNSPKTNDLLSNLGIQIIKTTGSGYVSDLNFVLSKLDEPALVVSGDLPLLD
                     AQIVKQIISAHKKDATWQSFLVTKSLLDLLNLRSNFSVFFEGKHCYYTGISIINPQKI
                     SQKVEESYTILDDKRIALNLNTKHEYDLLKNA"
     gene            complement(52201..52926)
                     /gene="cobS"
                     /locus_tag="VGA92_04200"
     CDS             complement(52201..52926)
                     /gene="cobS"
                     /locus_tag="VGA92_04200"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX9845646.1"
                     /translation="MFRQVGSVFSFLTIIPTGNSDLQTVARHMYLFPIIGIAIGLMIG
                     STAYGLSLFLEPLIVGLLITAALVLITGIHHTDGLSDFADGLMVRGTKQKKLEVMRDP
                     SVGSAGIVAIVLYVAGTVIALSTIKGFEIFVAILAAEIIAKFSMVLLTSFGPSAWEGS
                     NSPFVDSMKNRKKLVIAAVITVGAIVVLQNNVGFLALGLGIVLTLIILVVSRRSFGGI
                     SGDIMGATNEITRVASLLVFASI"
     gene            complement(52919..53887)
                     /locus_tag="VGA92_04205"
     CDS             complement(52919..53887)
                     /locus_tag="VGA92_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9845647.1"
                     /translation="MILEPILIAVGALALDFAFGDPKNKLHPTAWVGALVARLVPLAK
                     DKNPTVEKLGGIGVTLCVTAIIATVLVTYEFFTKNMTDIISTIIIILIGSLLLKSTIA
                     IRGLERHGALVMEALSRNNLEEARQRLSMLVKRDTKDLDRNHVVSGVLESTSENIVDG
                     ITGSMFYFGIFGLPGAFVYRTVNTIDSMIGYKNTIFSNVGWFGANCDKILNYLPARIT
                     AYVMILAAVIIGADWKNALNTMRQDSRKTQSPNAGYPMAAMAGALGTRFEKINHYSLG
                     NGTTELDQNHFRSAILLMKVTCLLFCLLVTIPMISILYYLGWWIHV"
     gene            complement(53884..54705)
                     /locus_tag="VGA92_04210"
     CDS             complement(53884..54705)
                     /locus_tag="VGA92_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX9845648.1"
                     /translation="MIQGTSSGVGKTTLVAALCRIFANAGYRVAPFKSQNMSNYSYKA
                     DGFEISQAQAIQAIAARTEISPHINPILLKPLGNYRSSVILQGKFYKEMGAQTYYKKF
                     VLKKGLSLALDSFAQLKKNHDLIIIEGAGSPAEINLQKYDIANMRIAQKTGSSVILVA
                     DIEKGGTFASIVGTMSLLPKQHQKLVKGFVINKFRGDVKILKPGFRRLKQITSKSVLG
                     VIPLIDMDLPNEDSLDNKIHLFRKQNQKKMDREIEKLAKTVKSHIDIKTIEGLLQ"
     gene            complement(54717..55793)
                     /gene="cobD"
                     /locus_tag="VGA92_04215"
     CDS             complement(54717..55793)
                     /gene="cobD"
                     /locus_tag="VGA92_04215"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HEX9845649.1"
                     /translation="MKFAKNIATHTPVPHGGLYSIENPHPTIMDFSSNVNPLGYPTSV
                     KKLLGSWMEKIPVYPDHNSKKLKKRLSSYLGIPTSNLVVGNGATEVIYNFCRAIIGKH
                     TPVLIPIPTFGEYDAAVRLCGGKLQFFKTMNLELDLTKFMKKIPTNGVIFVCNPNNPT
                     GALISKKSIIQIAKYAKTKNTMVFVDECFMELTQTPQQSVIDQIQKLDNLFVLRSLTK
                     SFGLAGLRVGYGVGSRNLVSVLDKIKIPWNVSGLAQEAAVTALSEKGFLAKTRKLIKT
                     ESAFLKNYISGLDGFSCFETTTNFILIKTKTDSKRLQKKLLQKKILIRDCSTFRGLAQ
                     NYIRIAVKTHKDNLKLVRALEKLA"
     gene            55878..56957
                     /gene="asd"
                     /locus_tag="VGA92_04220"
     CDS             55878..56957
                     /gene="asd"
                     /locus_tag="VGA92_04220"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9845650.1"
                     /translation="MKKRVGILGATGAVGQEFILSLENHPWFEVTQLAASERSAGKKY
                     IDAIRDPDSGIVKWQVGGDVPRYVRDLMVVAVDQVKANELDLVFSAVESDAAREIETK
                     FAKDVPVISTSSAYRYDEDVPILIPGINDDHVELIEKQRKNHNWKGFVLPLPNCTTTG
                     LAITMKPLYDSYGAKRVIMTSMQAISGAGRSPGVSALDVTDNLIPYIPKEENKVRIET
                     AKILGKLKDGKIEPANIKVSCTCTRVPVIDGHTESVFVETTKPAKADDVKHTIEEFSH
                     HVSVQGLPSAPKDYLIVHQDPTRPQPRIDRELNGGMTTSIGRLEEDTIFGNGVKYVLF
                     SHNKKMGSAKGAVLLAEMLYKKGKI"
     gene            57043..57492
                     /locus_tag="VGA92_04225"
     CDS             57043..57492
                     /locus_tag="VGA92_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9845651.1"
                     /translation="MDELDRTILSELSEDASISIPKLSEKIKVNPSVVYSRIKRLTNK
                     KLIERFTIVVNDKELGYGVKALTGINMDTKFRDTIIDELFKIKGVREISEVTGRFDII
                     VTMYAMNLAEMHKLISEGIGSIQGVLASESFIEMKRRTKTMPYNHEA"
     gene            57543..58472
                     /locus_tag="VGA92_04230"
     CDS             57543..58472
                     /locus_tag="VGA92_04230"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9845652.1"
                     /translation="MQKAQTGSRIKTYYELTKPKIWYLLVFTAFGAAITAANIYNVPV
                     SPATWALMLFGVAAGSASANTLTNYHDRDIDAIMERTKGRPIPSGRIAPPKARDFGLV
                     LAAISMACAFAISYTAGFWNGVWAGIFMAFGLVNNVVVYSLTLKRRSRTNIVLGGFCG
                     GAPVMIGYAAVTLQGLWDLGLVMAGLVFIWIPMHIWALTLHFKDDYNKVNVPMLTAVQ
                     SEKTSARIIAISTLVMVLFSVVPFFLTTNTGEPVMREIYLYTAIASGVLMLVLSFWVI
                     AKPSEKASWMLFKFSSPYLAVLFIALMIDSSLR"
     gene            complement(58748..60298)
                     /locus_tag="VGA92_04235"
     CDS             complement(58748..60298)
                     /locus_tag="VGA92_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845653.1"
                     /translation="MRKAALAVVFLLFANLYPAFGNYGTPTSQEQDLYNKLLAEFEKL
                     TISKKDVSDTLKSFKEPIVISFPCDSTDTNNFVNATITTKLAHFDDSLASHEGAVTGG
                     IYVWKCSITFSAGKLTINCDNGFMLNANAVLQTTEKDPQVKLAEDLVIFYHELLHGQL
                     MIDAIKSSSAWRDDTCNKEFQDELDYSYTDSEHQIITPLQTELASQLIENIGGIFQLE
                     EITPQETANGAFTKKVGSLYDYPEYVKSGISISARSYNIADIQITSQKNDIMISGNLN
                     DKSQSGIVWLYIFGKEQEESADTPITETQNESPSVSQQTEIPSWIKSSANWWADGMIE
                     DNDFVLGIQHLINVEIIKVPQTTQGTQNTQQIPYWIKNNAKWWASGLISDLDFVQGIQ
                     YLIRNGIMQIQVTSSSPTQTVEESAPEPQKLSFVSVNADTFEKQSHQTINMQITGQVE
                     NFKSGTYVFLTITKPDQSSYDLKGILTNKGQFTVPVMLDANSQAGKYTIKAKYNNVAF
                     DTTSFTVK"
     gene            60746..61237
                     /locus_tag="VGA92_04240"
     CDS             60746..61237
                     /locus_tag="VGA92_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845654.1"
                     /translation="MSGKQYQDSKKNQVPIHSEQKVQNKMTQTNESQKDVFSVYTKSF
                     DKLKSAFERTSTQSLQSYTTLNQEILSTWSSFIHTVASIQQNFANKVGVNASLPETSA
                     TVIQDATDAFVKTVETNSKVVQTALDATVQNIKTVNQNTNAFAELNQNIINSWISAWK
                     PRN"
     gene            complement(61289..61705)
                     /locus_tag="VGA92_04245"
     CDS             complement(61289..61705)
                     /locus_tag="VGA92_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845655.1"
                     /translation="MDKESKSTRVSQDGNATDLWNAYSKALEMWVKSFDTMQEAATNA
                     FKLYLQGFETATRQSNFDEMKKYNELWQNISKQFEQYNPYSWSIKAWDDLWKESGFAS
                     FKSFADYWQNMWQNFAKDAEAKSQEALKQLSKQNTS"
     gene            complement(61828..63285)
                     /gene="glnA"
                     /locus_tag="VGA92_04250"
     CDS             complement(61828..63285)
                     /gene="glnA"
                     /locus_tag="VGA92_04250"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9845656.1"
                     /translation="MSVYNLSKIENVFKKIQDNNVDFVEFWFVNIFGELHSLSIPSYS
                     LNEDHFKNGLQKLDASSIKGFKSVDDSDLLLMPDISTFRILPPYYDNGNRQNACVFAD
                     IYDKKDQSISRFSKDSRSILAKTRDELRKFGIDEARFGPEIEFFIFDSLKMIPSTTNA
                     ISSSGTIGYSIKSMEAPWSQEDTSIDLKGGYYMAKPKDTMDIVRKDICDDLHQYFGIQ
                     VEAHHHEVATSGQCEINVQHNEMLAMADSVISIKNMVKVKAKQMGKVATFMPKPIYGD
                     NASAMHIHHSFSSNNENLLYDPNDQVTALSQTARYYIGGILDHAEALCAISNPTTNSY
                     KRLVPGFEAPVGICWGLSNRTAAIRIPKVFDDDKNKKRLEYRVPDATSNIYLLEAALV
                     LAGLDGIKRKLDPGSPVEQSVYRMTPEKRRNYNVRFLPSTLKEALDALQSDKKFLQDV
                     FTGEFLDTYTTIKYEEYSLFAQTPTVWEVAMYSSL"
     gene            complement(63288..63548)
                     /locus_tag="VGA92_04255"
     CDS             complement(63288..63548)
                     /locus_tag="VGA92_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845657.1"
                     /translation="MMEMQVFRAQKTNPNLKSSLLRISDLAFFAGITISCFGEVALIG
                     YAINLPQMYYRGLLGNGIDFNTALLLLIIGNLILFAENKLKK"
     gene            complement(63565..>64241)
                     /locus_tag="VGA92_04260"
     CDS             complement(63565..>64241)
                     /locus_tag="VGA92_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845658.1"
                     /translation="NHPIELSTRAIQEAIRRIRKTGAQIRTQSPVLAHINDDPQAWAD
                     MWQKQVSLGCIPYYMFVTRDTGAQEYFGIPLVRAQEIFRDAYQKVSGLARTVRGPSMS
                     ANPGKIQVLGVVDVGKKKAIALRFLQGRNPKWVQRPFLAKYDEKAIWIDDLEPFSGKK
                     FFYEDELKQILKSSKEAVKKTLDQTKKEQPKKIPVLVSIKNTKMYTFEDEKPLDKWHI
                     GKKNFS"
BASE COUNT        18368 a        12838 c        12654 g        20381 t
ORIGIN      
        1 agagggattt gaacccccgg gtgcttgcgc acacaggctc tcaaggcccg cgccctaccg
       61 agctaggcga cctctgcggg attcgatgat agtaactact taaaatttgt tttagtatta
      121 agacgatata atttagaatt aaaagaaaag aaaagaagtt tgtttctagt cgtgtgcgtg
      181 tgggtttgtt ccgccttcga gcttgacata tgtaccagcc gctttttcgt ggtgttcaag
      241 atttgattga tcaaccttga tagccagagg tggacaaact gaaacacatg ccatacacca
      301 gatgcaatca tgttctctga taggatctgc tttatcggtt ttgtctttgc gttcttcttt
      361 tactgcagag cctgtacctg caaaggtttt tccaattacg tcttttgctg gaatatccat
      421 ttcagttctg taccattgga aaacttgtac tggacaagcc tcaatgcatg ctccgtcagc
      481 tacacaagaa tcccaatcga ctgcaaccat tgtaccgctg actcctagtg ggacctgttt
      541 ttcgccgtgt tttttatagt cggctatgac agcttcgtct gagaaagcct cagcgtcgct
      601 tcttcctggc ccccacatga agtgatagtg ttcgccatca gaatgcataa tttttccaat
      661 tggttttagt ccttgtggga aattttcgtc tattggcata tgactattct tagtgagatg
      721 ttatttaaat ctaaacaaaa ttagtataaa ctaaatttgg ctctgacaaa aatgacagat
      781 tataacactg ttttttagtt gatcgccctt acgttcatat tttttgattt tacagttgag
      841 gagttggaaa tttacaagta cgatgtatat cgaggcccaa acattggcat ttataccaaa
      901 gtaaatgaca gttttgtttt tgttccaaat ggctttgcac acacaaaagc aactacctta
      961 gctacgtacc taaaggtaga atacctgttt acctcagtag caagtacaag gctcattgga
     1021 accatgatgg cagtaaacaa tcatggcatg ttgctgccaa gcacgtgctc agaatacgag
     1081 gtgcagtatt tgaaaaaatc aactggtctt aatgttggga ttttggacac aaagcacaat
     1141 gctttgggca atctaatgtg tgtaaatgac aaaggcggaa taatttcacc aaaaattcca
     1201 aaggaagtgc taaaaaacat tcaggacata ctaggaatag aggtgattca gaaaaaaata
     1261 gcaggttacc atcaggcagg tgccatgatt tgtgcaaaca atattggagg cataatccat
     1321 ccagaaaccg acgatgacga cattaaatca gtttcagatg ttttaaaggt caaattagag
     1381 ccagccacca ttaatggggg aataccatat gtctcatctg gaattttggc aaacaacaag
     1441 gcagtagtgg taggcaatct cacaaacggc ccagagatca tgatgctaac aagggctttt
     1501 acaaattaaa cagtgatttt acatcagaaa ataacgagtc gactaaagcc aaagtttcct
     1561 ttccatcact catgtttttt acaacgactt ggtttgttga gtattctttt ggtgcaacaa
     1621 ttacaacaaa ctttgaattg gatgcgtttt ccatttgttt tttgaatggt ctgccaatga
     1681 gatcaatttc cgtgtgtacg cccatatttc gcagctttga tgctatgttg attgctggtt
     1741 tgagcatttc ttcgttaaca aataaaacgg agatttgtgg gtttgaatcc atattttgta
     1801 gcccatgact ttccaacgat agaataatgc gttcaactcc acctgcaaca ccagttgcgc
     1861 caagatcgtc tcttccaaag gcttttggta gactatcata tcttcccccg cccaccaatg
     1921 cgccaacatc tgaggagctg tcaaatgcct caaaaaccac acctgaataa tagtcaaggc
     1981 ctcgaactat gccgaaattt accctgatgt tttctacacc cctgttttta agagtggaga
     2041 acagttccct tagttcaacc catgcatcca ttcctgacac ctcaaaggct ttctcaactt
     2101 cttgtggctg gccctttatt tttgaaaatt ccaagatttt ttctagtttt tccttagagt
     2161 agcccttttg ttggtattct tgtaggattt cgtctttttt ctttttttga attttgtcaa
     2221 ttgcccgaaa tatatcccca attagctgcg gatcgtttga ttcaaagacc tttgcaacta
     2281 ctgcctcaac taattttctg tgtgaaatat ctattatgat gtttttgaga tttagttttg
     2341 aaaaaaactt gcttgtgaat tcaattattt ctgcatcaga ttccaggctt ggcttgccat
     2401 agatttcaag gtcccattgg tgaaagtatc tgtatctgcc tttttgtggt tcgtcgtatc
     2461 gccatacgcc tccaaaggca gcaatctttg ctgggagttt gagtgatttt tgcgaaacaa
     2521 cataacgtgt tagccctata gtgaaatcaa accgtagtgc tacttcgcgt tctcctttgt
     2581 ctttgaaaaa ataaatttca tctctaatag taggtccgct tttagcttca ataactgaga
     2641 caagttctat tggcgaagga tccataaagt tgaatccaaa tgtcttacag gtctccagaa
     2701 atttttgtct gatgtgttcg atttttaatt gctcgttact ttcaaaatcc ttcattcctc
     2761 ttggaagatt caactggtga agcttcctag ttaatggatt tagatgttgc tctatactgc
     2821 acatcgtttt actattataa tagagagaga tcaatctact aattgagcta caaatatctg
     2881 gagcatgcaa cagacgcatt catcgaagtc aacgcctcca atttagaaga agcttttact
     2941 cttgcggcaa aatcaataat tgaaacaatt cttgatatcg atgcagttga ggcaaaaaaa
     3001 gaacgacaaa ttacagtaac tggcaaggat ctcaactatt tgttgtataa ctggcttgag
     3061 gaattgataa ttttgaccat aactgagggt tttgcttcaa aaacagtcaa gctcaaaata
     3121 gaaaaaaacg cggaatacag gattttggca acgttggcag gcgaagaaat tgattgtaaa
     3181 aaacaccatt ttaagatgga gatcaagtcg cctacatttc acttgatgga aataaagcag
     3241 aatggtgatg tactgatgcg ctacctccta gacctataaa attatagaca tatgtcaaat
     3301 cttaaagttc atggaagacc cagaaataga aaaaattaag caaaaaaaac ttgaagaact
     3361 gctcaagcaa caacaagaag cgattgccca aagccaagta gaggtaattg atcttgattc
     3421 agctaatttc gatcaggtca tttccactca aagtctggta ttggtagatt tttgggcaga
     3481 atggtgtgga ccatgcaagt taatgcaccc agtctttgat cgaatggcaa aaaaatatcg
     3541 acatatcaaa tttgcacgag taaatgttga taaatgccaa gacgtttcaa tgcggtttgg
     3601 ggttcaggca ataccaacct tcatcatgtt caaaaatggc cagccagctg acagaatgat
     3661 gggagccgtt ggcgagcctg gcatccacat gattgctcga aaatatcaaa tgtaaccata
     3721 cgcagaatcg atttttcgct ggattgccac aatttcattt agtactgttt gttcttctgg
     3781 ggaaagtttg tgggtgaatt tttcaatcaa ttgttttgct tcagaggatc gtggtagtga
     3841 ataaaataca tcttcctcaa agatatgcct tccaatcatc acgtcttgta tagagcaagc
     3901 tacctcttgg ccgtgtgttg ccgactcgat tgtcttttta ccatcctgaa gctggtggat
     3961 tattccaatc tttttaccat ttttgttcat gattggaatt ttttgtctta cctttcccac
     4021 gtcaacacgt attccaaata ctgctggatt gctgtttcta aagatgtatc ctttcagaaa
     4081 agtgaacttg caaacaggtg tcagctcgct aaatattgcg ccttcctcgt ttgctgtgtc
     4141 ctcgtctacc cactgcacat agttatcaat gaggctatag attattttgt cattgaagat
     4201 tttgacatga tttatttctg attcttcttg tgcatcaggt agcgttcgta cattaaaggc
     4261 caagatcacg ccaaggtgtc tgtctttatc tttgatgatt tttgcatcca ttacgtcacg
     4321 gtgactaaca tggccaatgt ctgcttttgc aattggtatt tgctgtctcc ttagcatttc
     4381 tgttagtgcc tccagagacc caattgtatc gcattttagt attattccgt ttgtttgagt
     4441 gtcgataaat acagacttca tttcagattc gatgatcttt ttgaactctt cgagttttcc
     4501 gtcatcattc gtgacataaa ctgtactgcc aggaagtaca ccgtccaaat caggtgatgc
     4561 aatctttatt ccagcagctg cctccacatg atcaactggt ttgaatttgt cgcgaggatc
     4621 acgcatctca tcaagtggtt ttggcaaaag cattgccttt ggttttgtca caatgactgt
     4681 gtctcttttt gctacaacta cgctgtctcc tttttttatc tgaccatcaa ttagtatcat
     4741 gtttgccgtt gttccaaggc ccacctcgtc gtttacttca agaattattc cgcggcttgg
     4801 tttttcttcc tgtgtaagcc ttttttgcat gtattgttgt gttagaccca ccaaaacagt
     4861 caaaagttct ggaattccca cgccaactct tgcactgact gggacgattg ctacttcttt
     4921 tgcaaaatct tgcacacggt agaatgcttc ggattgaaaa cccaaaatcg aaagcgtccc
     4981 aacgacgttg tatatttgct catcaagtgt tgtttggata aactgatctt gttgttttat
     5041 tgcctgcgta atgtacggtg tatcaatttt tttccatcca ggtatctggt caactttgtt
     5101 gagcgcaatt acaaatggaa cttttcttgc ctgaagaatt tttaggcttt cagtggtttg
     5161 tggctgaaat ccgcggtttg tatccactac caagattgca atgtctgctg cagaaccacc
     5221 acgagttcta aggtttgtaa aaacttcgtg tcctggagtg tcaattacca aaaggcctgg
     5281 aacttcgtat tctgattttg tgagtctttc gtacagctgc ccgcacattt ttttgattgt
     5341 ttctgtgggc agaaagcttg cgcctatgtg ctgtgttatg ccgcctgctt ctctactttg
     5401 gacgccagtt ccacgaattt tatcaagtaa tgaggtttta ccagaatcta catggcccaa
     5461 tacggccaca atcggttgtc gaattctcaa ggcaactcac tgaaatgcta ccctcgcttc
     5521 cttttcaaaa ctttcttttg aatccgacgc gtgaatgata ttgtcagata ttccaaggcc
     5581 aaagtcacct cgaattgagc cggcgactgc ctcatacgac ttggttgcgc caaccaaaag
     5641 acgtgttgta gcaacagcgt tgtttccttc aattattgca gctacaactg gtcctgatgt
     5701 gataaatgag acaagttcac caaagaatgg ttttgtgctg tgaaccgaat aaaattcctc
     5761 tgcttgggat tttgtaaatg taaacatttt cagttttaaa attttgaagc ccttgctttc
     5821 aaatcttgag atgatttggc caactagttt acgctctact gcgtctggtt ttattatgaa
     5881 aagtgtttgt tctgtcaatt tattctaaaa ctactttttg ataattccgc ccttttggta
     5941 ttttttggtc cacttgagtt tacgcggatc gcgttttagc accaacatat tttttttgca
     6001 ttttggagaa cagaaccaca atactgtgcc gtcatttttg actagcattg tgcctgatcc
     6061 tttcgaaact ggcctgtcgc aaaaactgca tggttttaca attagactca attattcatc
     6121 actttatctt cttggcttca cgttctgttt cgcgaagcat caaaatgtca gatagcctta
     6181 cagagccttt tacgtttctt gtcaagattc ttcccttgtc tcttccgcct agaattttta
     6241 ctctaacttg tgtaatctcg cccgcaattc cggtcttgcc aacaatttgt attacttctg
     6301 atggtactac gtcttctgtt tgggaagtac tcattgttac ttgccacctt taagtttctt
     6361 aattgaagat actacttctt caacaatgtg gtttgcgtct cctgcgtcaa ttattgctgc
     6421 tgctgcagaa gtaacatcta tgccaagtgc ttttcctagc tcttgtttgc ttgggacaaa
     6481 tgcatatgca gcgttttgtt cttcgcagat aattggaaga tgcgcgacaa cctctggagg
     6541 ttcgacatct tcggcaataa ctattagttt gcttattcct cgctcaattg cttttgttgc
     6601 ctcgtttgtt ccttttctga ctttaccact ttgtgaagcc acacgtactg cctctagtat
     6661 tggatttatg aggtcagctg gcgtctcgaa ttttacatag taagccttag ccaattaaat
     6721 cacccatagg gatcatctcc attccttggg tttttgggta tcgccttatt agtgttatct
     6781 gccaagaatt gcctttacct ttttgaggta ttttgacatc aaaccatcaa gtctttgttg
     6841 cgagtccgac tctgcgtaaa ttcttgcaat tggctctgtc ccacttgatc gtatcatcac
     6901 ccagtttttg tcatcgaata ttatcttgat tccatctgtt gtgtcatagc tttggtgttc
     6961 ttttttgagt tgtgcaatta ccttgttggc ttgctgtttt gtgcacaaaa ccttgtcttt
     7021 tgttgtaaag gatttgggaa tgcggtttag ctcccccgtt agagacttgc cagagtgggc
     7081 aagaaggtcc aacattaacg caagcgtcat gcagccgtct cgtacttggt tatgttttcc
     7141 gtacataaag cccccatttt cctcaaagcc gacaagggct ttttctggca ccatccttcg
     7201 ggatacctct acgcttccga ctttggttcg aattaccttt gattttgtct tagatgcaat
     7261 tgcttcaatt acggagctgg agttaagaca tgttacaacc tttgattttg ggtttcgttt
     7321 tagaatgaaa tttgtcagca gcagtgctga cttgtctccg ctcaaaatct ctcccttgtt
     7381 atcacaaaat atgcttcgat ccccatcacc atcaaatgca atgccaagat ctgctttatt
     7441 ttgacgtact gttttggaaa gtttttgtaa attgtctggt gttggctctg atccccttcc
     7501 tggaaatctg ccatcgattt tttcatttat ttggaatatt ttgcagccaa gctccttgca
     7561 gagaattggt gctgaaactg cctgggcccc attaccaaga tctaaaacca ctttgaactt
     7621 ttttgatttt atctttgcca aatttacctg cgatttgatt ccatcaagat atgttcgcag
     7681 tactcgtgat tcggttgcag ttttgccaag ttttttgcta tattttatcc atttttttcc
     7741 aaagtaaatt ccttctatgg tttgctcacc atctcttgag agttcaacgc cgtctctggc
     7801 aacaggtttt agtccattgt attgtggcgg gttgtgagac gcagtaatca taattccgcc
     7861 gttatatcta agagtttttg ttgcatattc aagggcgggg gtgggaacaa ggcctgcaat
     7921 gttgcagcta ataccacagg agttcagagt ggaacatatc accttggcaa tgactggact
     7981 ggagtctctg ccatcatagc ctacaagaat tggccccttt ttgaaaaaag ttgcaatagc
     8041 cattgtaata tcgtggatga actctaaggt cagatcctca ccgaaaactc cccttattcc
     8101 atttgtgcca aataattttc ccatcgcttg tggttttcat gagctaaatt tgtgctttgt
     8161 tagatttttg tgggatgcaa gttatccaat ggtaagcaat aagtttgtgt aaaattggag
     8221 catcagtgcg ggggttgcca agcctggtca aaggcgtaag gtttaggccc ttatctctta
     8281 ggggttcgtg ggttcaaatc ccacctcccg cattttcgct taaaaccaaa acagcattaa
     8341 taaagtgatt ttgtaacaaa actctgtgaa aaaaatcaga gtaggaatca ctgttattgg
     8401 aaaggacaaa gagggaatcg tagctgcgtt tacaaatttc atttttcaaa aaaacggtaa
     8461 cattgagaag gtaaaccaga atgtcataaa gggactcttt ggaatgcacc ttgaagcgtc
     8521 attttcaaaa gcgtttaaca taaaggcatt tgatagggag cttcagggcc taggaaaaaa
     8581 gctcggcatg gaagtaagtg tctatcacga gataaactcg caaaaaaaca tcgcaatctt
     8641 tgttacaaaa gagtctcatt gtcttagaac tctgctcgat gcaaatgcaa aaaaacagct
     8701 taaaggacga atcaatgtca tagttggaac acaaagaacg cttgagcctc ttgcaaaaaa
     8761 agcaagaatt ccatttattc tagttgatga aaaagaccaa caaaaggcag aagacaaact
     8821 agttgaaatt acaaaacgtt acgacgttga tttggtagtt ttagctcgct acatgagaat
     8881 aatgactcca aactttgtat ggagataccc acaccgaata atcaatattc acccctcgtt
     8941 attaccagcg tttcctggag cgtttggtta tgcacaggca tttgagcgag gaacaaaaat
     9001 tgttggtgtg actgcccact acgtcactga ggagctggac caaggaccaa taatatttca
     9061 agattctttc aaagtagatt catcagatac acttgaggtc atccggcaaa agggtcagaa
     9121 gctagaagca gatactctgc taaaggcagt caaaatgcac cttgacggaa aacttgaggt
     9181 aagctggaga aaagtgtaca caaagtgaat ggtataaagg atttttctga tccaaatcta
     9241 cgaaggctga tcaaaaacaa aaggcagatt gcgttgattc ccgtaggctc acttgaacag
     9301 catggttcac acttgccaat atctacagat tctgatatcg taacagaagt agcagaaagg
     9361 gttgcaaaaa aatgcaatct tttgcttttg cccacaatta cgtacggggt ttcgtttgaa
     9421 cacgcgccgt ttttcaacct tagtataact gcgcaagcct tgcagaaatt gctcacagac
     9481 ctatgcattt cgcttgccca aaacaaaatt tacaagataa tcatcttaaa tggtcaccat
     9541 ggaaatcaca agccattaaa ggcactagaa ggcaaaattt ctaaaatttc tggaaagaag
     9601 gtcaaggttt ttgtgttctc gtactggcat tttatgaaaa gagagtttga tcatgcgggc
     9661 tttgctgaga catcactcat gcttgccatt tcaagcaaag tagacatgaa gctggcaaaa
     9721 agaggactga tcgcagatag gcttagtcca aagcagaggg caaaaatttc caagattgca
     9781 tctgctcact ttattaaagc aacgaaaaac ggtgtctggg gcgacccaac aaaggcggca
     9841 aaaaaagacg gagacagaat tttgtctgaa atagtgcgaa atattactga aacagtgtca
     9901 aagttggctt actgattaaa actctaattt acaccaatga aattttaata taatccctag
     9961 ttaggagagc caataagtga attaaatgtc agttgacatg gcagtaaaag tactggacga
    10021 aagcatcgac aaaatagttc taatcaagct caagggcggc aaaaccatca gaggcaacct
    10081 cagaggtttt gaccagcaca tgaacctgct actcgacagc tcagaagaga tcccttctga
    10141 aggcgagaca aagagcatag gaaccattgt tgtgcgagga gataatgtag ttatgatatc
    10201 tcctcctcct gcgtgatgac atatgactag aggaactccc tctatgggtc ttttcacaag
    10261 gaaaaggacc cacatcagat gcagaagatg cggtaagaat tcttatcaca agcgtagcca
    10321 tcgctgttca agttgtggtt ttcctgatgc aagaatccgc aaatattctt ggatcaaatg
    10381 gtacgcctaa atggaagttg cactaatcat cagttctatt gctggggcag ctactgcagc
    10441 taccattaac aagtttccaa aaaagaacaa agtttcactc ataggcgcaa actctcacat
    10501 aaaggcccaa atcgatacac taaggataga aaaagaaatt ctaactaaaa caatttcaag
    10561 attatacaca gacagtcccg atttaacaaa gattcagcgc gacaagcttc tcttacgata
    10621 tcaacaccaa ctcggagtca ttttagctaa aattgagaaa ctagaacaag caagcaagca
    10681 tcccgatctt ggtcctcttg gcgatggcct aatcacacta atggatcaaa aactttctgc
    10741 ccttgataaa aaactcgtag aaatctctac aaagatttca gttgcacaaa ctcagctaga
    10801 aaaaccagaa atcaaaaagg aagaaccaaa aaaagaggca aagataattg ttgagaagcc
    10861 actcgaaagg ccagaaattg aaagcactca aagcatagag gagattatcc agtcatttgc
    10921 aaaaccttct aagagattag aaataactac gcttacggaa attccatcaa atgtttccgt
    10981 tgccccgcca attgagacaa aaccaacaat tcaaccgcaa gctcagacag ttgcacaacc
    11041 tcaaccgcag attcagcaag tcgagattgt gcagatcacg caaccagaac cagcaaaaca
    11101 agaaatcata gagataattc agcctcaaat aaagcagcaa ccggtcatca cagagccaat
    11161 tgacattgaa aaacaggtaa aatcagaacc accaaagctt gaattcagaa atgctgaacc
    11221 aagtcaagaa caagaaaaaa agaaatcaaa gataaacatt ccagagcctg aagaaatcga
    11281 agacgacgaa gatgatttag attctatcaa acgcgagata atgaagacgt tatcaaagct
    11341 agagcaggct gaggttgaat aattgtcatt tgatgatgcc atttcggcat tgtctaaaga
    11401 tgcgctaaaa tttgtaaaag acaataccgt tttagggctt ggtagtggtc gagctgccac
    11461 cgcatttgtc aaggcattag gccaacacat caaagaaagg aaattgacta tcaaggcagt
    11521 tccgacatca ctacagatca agcttgttgc agaacagagt ggaatccatg taattgatgc
    11581 ggagcaggtc caaaaaattg atgttgtttt tgatggtgct gatcaaattg attcacaaaa
    11641 aaatctcata aagggtgggg gaggggcact acttcgggaa aacattttga tttcttctgc
    11701 aaaacacgtt gtaattatgg cagactcgac aaaatttgta aaatacttta atcgaacagt
    11761 tccagtagag gttcatccgt ttgcaagaag tactgtcaaa aaatttattg aaaaacttgg
    11821 tggaaagcca aatcttagaa caatagatcg tggctatcca tttatcacag aaaatggcaa
    11881 tataatttat gattgtgatt ttggtacaat caaaacacca aaacctttgg cgcagaaaat
    11941 aaagcaaatt ccaggtgtac tcgaagtagg aatctttact cgaaaacccg atgttattta
    12001 caaggctaag gaaaacggca aattcgagat tttagcctaa agcgttcgct ttaccagctt
    12061 tccgtgacct ggtttaccta caacttgcat attttctgct acaagtttgc ctcgtaccat
    12121 cgtagtttgt ggccagccct taaggttcca gccgtcatat acgagataat ctgagaaggc
    12181 accaaaaaag tcggcgtcga cttttttttc tttttttagg tcaattaggg taatatcggc
    12241 atccgatcct ttttggattg ctcctttttg tgggtacata ccaaagattt ttgctgcatt
    12301 aaggctcgtt agctcaacga gttggcctag attaattcgg tttttgttta caccctcaga
    12361 tagtaagatt ggaagtgcag tgccaattcc agggaatccc gcaagtgaag accacacgtc
    12421 ttctccaact tttagcttta gtctgtttgc tacatgatct gttcctatag tatcgatgtg
    12481 tttttgtaaa attgcattcc aaatttgatc aatatctgct gagcttctaa ttggtggcat
    12541 tacttttgcc agatatcctt tttgtttctc ataagaaaga gttaggtagt gtggacaggt
    12601 ctctacaaaa attttcgttc ctttttttct ttcttcttgt attgcggcaa ttgcagcagc
    12661 tgaaccaatg tgaacaaaat acattgttgt tccaaactgg cgcgcaattt ctgaaatttt
    12721 tttaatgctt tttacttcag agtctggtgg tctgctctta gaccaagctg aaaggccgtc
    12781 ttgtttgtct tgttttgctt tttttattcc acattcacac atctgatagt cttctgcatg
    12841 aactaaaact ggacagccaa gctttgctgc atttgataat actttttgta ttgtgcttgt
    12901 ttcaagctgc acttgcgtct caaataatcg tgttgcacca ggatccatgt ccatgtaaac
    12961 gtggccaact tctcccccaa gattcatgta taatttaaat gaggtgattc cgttttctgt
    13021 acaaaacttc atttcgtctg cctgcttttc atcaaaaatt gtggcatgaa ttgcataatc
    13081 gacataatgt gaagtaccag acgctgcaag ttgtgctaaa agatgtgtct tgtaggaacg
    13141 cccaagcctg agcatcctca tcatggtggt aactccaccc agcgctgctg cacgtgattc
    13201 tgtctttgca gactcttcaa ttggtgaata aacgccataa tgtacgtgtg tgtcaatcaa
    13261 tcctggaatg gcaactaggc cttgcgcaga gattttgtta tcacattgtg gaatgtcact
    13321 tgtaaaatct atgatttttc cttcatctat gacgatgttt ttttgtaaaa ctccttttgg
    13381 cgtcattaca tgcggttcta caattacaga atctacagtc aatcaatttg attgagaaaa
    13441 tccttctatt atgctttgat agtgcttacc agctttgttg atatgcaagc tgtttttttg
    13501 tgagcctatc gattttgata ttcatggaat tcagtgcgtc gagcgctact tggtcatcaa
    13561 tctcctttgg caccccataa actcggtttt tcattttttt atgatttttt agaatgtata
    13621 aaattgagtg aagctggttg gaaaatgaga gtgccatgac ttcgggcgga tgtccttctg
    13681 aggcaatcaa gtttgccaca cggcccttgc tgatcagata gatctttttt ccatttggca
    13741 agatgcactc atcaagattt ggccttacct gctttactga cttggagtta tttagcaaaa
    13801 attttgtatc aacttctaca tcaaagtgtc ctacgtttcc aactattgca ccatttttca
    13861 ttgcaagtag gttttccttt cttattacat cggtcatacc agtgcacgtg atgaaaatat
    13921 cgccaagcct tgaggcctct tttattggca atacgtcaaa gccgtccatg tgggcttcta
    13981 atgccttgat tggatcaatt tcagttacaa ttacctttga gcccatgcct gcacatcttt
    14041 gcgtaacgcc ttttccaagc caaccatagc cacacacgac aacacgcttt gaggcaaaaa
    14101 gcagattcat cgtctttagg agcgcatcaa ttgttgactg gcctgtacca taccgattat
    14161 caaacatcat ttttgttctt gcgttgttaa ctgcaattat tggatagcgc agttttttct
    14221 tttgttgcat tgccagatac cgactaaccc cagttgtggt ttcctctgtt gcgccaaaaa
    14281 tcttcagatt cttgaatttt ttatcaaaat gggcctttat gttaaggtca ccgccatcat
    14341 cggttaggat ttgtggtttt tgtgacaata cttgattttg acaccactcg tattcggatt
    14401 ttgattggcc agaccaagca tacacattga tcccacgcgt gtctaaaaac gcagcaacgt
    14461 cgtcttgtgt agtaagtgga tttcccccgc aagctacaac ctctgcacct aattgttttg
    14521 cacctactag caaaactgcc gtctcctttg ttatttgaag acaaaagcca agtctgattc
    14581 ctcttagagg tcttgatttt tctagtttct taatagagtt tgttagaatt tgcatgtgat
    14641 tttttgccca ctcgtattca aattgtcctc tatctgccaa acttggatct ttgattttgc
    14701 tcaatactac aaaattggac aagctgtaaa ataatgattt gttagctgga caaagaggta
    14761 aaaatagagc aatagttctg ctaatccaag ggctggtggt ttagtggtat aacgctgcat
    14821 tcgcaatgca gaagtcaggg gttcaattcc cctccagtcc actattaaat ttgtgattac
    14881 aattctttat ttgtcccaat taattcccaa gaatttccat catctaggct tctgtaaact
    14941 tcttggaatc catcaggcga atatccggca acatatagaa tcttgttttg aacatcagtt
    15001 gtgatgctca ttatagttag attcgtagta ctgactttat tccaactctg tccagaatca
    15061 gtagatcttg aaaggccaaa ttgatcagtt gaagcataaa gagttccgtt ttcatcaaag
    15121 atcaaggcaa acaccaagag ccctttgtat tgctcaagtt gtatccaagt gcctgcccca
    15181 tcgttggact ggtaaatccc ctttccagtt cctgcaaata tcacatcagg atcagttgga
    15241 gaaattgcaa gcgcggaaat gtaatcagga ggatcaagtt tttgccagtt taatccagca
    15301 tcaacagttt tgaataaccc tcttcctgca ctgtcaaatc cgattatcat atttggattg
    15361 gatttggtta ctgccattgc atgaaaatca actggtggct ctaaaattgt ggtaacagtt
    15421 tgccacgtct ttccaccatc cgtgcttttg acaagaccca tgtttccgcc agtagaagga
    15481 tgaccacttg cataaagcgg cattccggtg tttggaggtg cattaaatgc catgtaatcg
    15541 gctctttgct catctacttt tactggtggc cctccgttta cactttgata aaagtctcca
    15601 tgtgttgcga tgtataaaat gcgtctatct gtaggatcta ttcccaaacc atgtatatga
    15661 cgccaaaata ctgatctaga attaccattt gattctgcat ctagatttgg cactgtaact
    15721 gagactagcg caactgctgc agcaattcca gcaactatag caatacctac aatcaaaaca
    15781 aacagttttc ctgatttagc tgtcaagtat aatcaccttt tttcaaaatg tatttgagca
    15841 ttttaccaat tccagatttt attttgctat gactccaatg tccatgtgat tattcatcat
    15901 agaatgcatc gattttgtag gcgggattac atgggtatga attgtataaa ctcctttctt
    15961 gtctggaata aatgtgattt ggtatttacc atctccaatc tcttttgctg caaacattgg
    16021 agtaaccatc tccattgtgc tcattgatgg ccccctctca atcattattg aaacgattcc
    16081 atccaaaagc ggttgtttgg ttgttttatc cttgactaaa atggtaattt ttgcttcttt
    16141 gcctgcctga acaagttgag aggatgcagg cgatatagtt acgtcttttg gtggtttatt
    16201 catcatcata ccacacatgt tcatcataga cgacgaatgt agtttttctt cagctaaagt
    16261 ttgtgaaagt aggtaactgg cagaaatcaa aattcctcca ataatgacta tagatattat
    16321 taatgcgttc atataacatc tgttttgata aattttggtc tgtacctgcc aagcaatatc
    16381 gaatttccaa caacagttac agagcttatt gccatggcag cacctgcaaa aataggaagc
    16441 catgagaaca tgcctaagcc aagaactggt acaaagattc ctgctgcgac tggaattaag
    16501 ccagtattgt aggcaaatga ccaaaataga ttctgtttta tctttgaaac cgttttcttt
    16561 ccaagctcta atgcaatgaa aacatccctc atgtcgtttt tgatcaggat tattccacct
    16621 gtctcttttg ctacatcagt tccagaacct attgcaattc ctagatccgc acttgcaagt
    16681 gctggcgcat cattgattcc atctcccacc atagcaacca ccttaccttc tgacttgagc
    16741 tttgagatga cctgctcttt ttgatgcggc aatacctgag ctattacctt ttcgaaccca
    16801 agcatagaag ccatcgcatt tgccgttctc tcgttgtcgc ccgtaagcat gatcacctca
    16861 attccatttt gttttagggc atcaattgcc tcctttgctg tttcttttat ggtgtctgag
    16921 atggcaatca tccctgcaag cttgtcatca attgaaacca aggttacggt ttttccatca
    16981 gcttcaagct gctgcattgt ggcttcggtt ttttctacga cgtaaatgtt gttttgttcc
    17041 attagttttc tatttccaat taagattttg tggtttgcat aggttgcttt taggccatgc
    17101 cccattatcg cctcaaagga atctgggttt gatactgcca atccttcttc ttttgctttt
    17161 ttgacaactg cctgtgcaag tggatgctca gaacctgctt ctgcgattgc agcaagcctt
    17221 agcagttctg tttttcccat ttccgcaaag tcaataatgt ctgtaacaga tggttttcct
    17281 tcagttacgg ttcctgtctt atcaaatact actgtgttta ccttttttgc gacttctaga
    17341 tactcaccac ccttgaacag aattccattt tctgcacctt tgcctgatcc catcatcatg
    17401 gctgcaggtg ttgcaatacc caaagcacat ggacatgcaa taattataac tgaaacaaat
    17461 gccaaaagcg cataatcaaa tccgacttca cctacaaagt accatccaag tcctgtgagt
    17521 attgctatag ctataactgc tggaacaaaa tatttagaaa cttgatctac cattttttgc
    17581 attttagctt ttcctgtctt tgcttcttcc actagtacga ttatttgcga aagaaccgtg
    17641 tcttgaccaa cttttgttgc tgtgatcttt aacaaaccgt ttttgttgat tgtagcacca
    17701 attacttgat cccccactga cttgtcaact ggaatgctct caccagtgat tgcagcttcg
    17761 tcaagcgatg acaaaccatc aattattact ccgtcagttg gtattctttc gccaggccta
    17821 acaaccaaaa tatcaccttc ctggacttct tcggttggaa tttctacttc ggtgttgcct
    17881 cttagtatct tggctttgcg tggttggagg tcaagtaatt ttcgtactgc ggaagatgcc
    17941 ttttcttttg tttttgattc tagtaatctg ccaactaaga tcaaggtgat tattatcgcg
    18001 gcggtttcga aatagactga gcctactgga aagaatccgt gaacaaatgt cactattgta
    18061 ctgtacaaat acgcagcagt tgttcccaaa gcgataagcg tatccatgtt tgatgctttg
    18121 gcctttaagc catcccacaa ccccctgtag aatctccatc ctatccaaaa ctgaataggt
    18181 gtagcaagag caaacattac gtaattgcta atatgcatcg gaagaaagct tgggaattgc
    18241 tctagcatgt gaggtagggt cagtaagatg attggtatag taagtgccat actaattgca
    18301 acatgcgttt tgagtttctt tagctctttt tctggttgtg tgaattcgtt aaggcatgat
    18361 gtggcacaaa aataatacac cctaccatca actgcatgtt ttatagaatt gtctttttct
    18421 tctacaaaca ttccacaaat tggatctttt gccatgcgta tcataggaat gatggaaata
    18481 taagaaccga gctttacaat tgtaaaatac agtttttata tgaaacataa ggtgatatta
    18541 caattgtaaa accaagaaat gcatgttgct ttattaagaa ataaaaaatt atggttaaaa
    18601 aattagtaac cataattttg tgggttttgt tcgaattttt ctttgcatga tgctgagcaa
    18661 agataaacta ttttattgct aacatgactg ataaattttg tttttttctc atcaaccatc
    18721 atgttacaga ttggatcttt aaacataaat aagatataaa atattagata gaaaaacaat
    18781 atactttaca tttgtaaaat cataaatagt ttggaaggca tgataattta atgccttttc
    18841 aattagatga tgtagacatt gcaatacttg aatcgttaat aaaagatgga agaaaatcat
    18901 ttagacagat ttcaagggaa accaaagtaa gcacgcctac cgtacaggca cgatatgaaa
    18961 ggctcgttaa cgtgggcttg ataaagacag tatcacccat tctggacctt ggcaagcttg
    19021 aagacaaaac tggagaacgc ttgaagaaca tacgaaccaa ggcgataaag catcatgatg
    19081 tgaagataac gaaaaatatg atgatcaaaa tgacttgtga ttattgcgaa gggccgattt
    19141 ctgaaaaacc aaacgtacta aaaattgcaa attttgagcg atttttttgc tgcacctcct
    19201 gtagaggact atacaaggaa aagtacaggg gaagaatcga atcattatca cgaaaacaga
    19261 taatctaatc tcgcaaacat tatacatctt tactattgat taatttcatg aaggttttca
    19321 acgcaaaggc cgcagcgcaa gactacatgt ctgctcacac cctagcattt tccacaccag
    19381 agcttacgct catgcgatat tcgttttggc ttggggatat gatcccagat ccaaaggaca
    19441 agtcaaacac aattccaagg atgctgtcgt atgttgaaga aaaagatttt gcgccagtag
    19501 aaattattga cgatgagcac tacgagccaa ccggcgcagt aagaaacact ggaatgtatg
    19561 gaaatatcaa caaggcaaca gacgcaaata caaaattctg ctcagagtgc ggcgcaaaaa
    19621 tttcccttaa tgcaaaattc tgcaccgagt gtggaacgac acaagactag gccctagccc
    19681 cacattttgt gcaaaacttg gctcccaccc tcagctcaga gccacaactt ttgcagctag
    19741 atgatccagt cgatcttggc atcattgcag aatcagttgt aggaagtgta cctgggctgc
    19801 caaatccttc ccttgcggaa acaattgatg ggggcattcc gggcgtttgg gctggcgtca
    19861 taggaacccg tcctgccatt ccaggcattc cccccatact acccattcca ggcatcaatc
    19921 ccggtgactg ggttgttcca aggttgccag caccaactct atcaagtgcc tttttcatct
    19981 caaagattac cttgactgct aaaaactcta atgctgaata cgctaccaca taatctccta
    20041 gtttttcaaa aaattccttt tctgatagtt ttccctgctt gtacagtgtg tttgcatcca
    20101 aaaacgactc gtaatatccc tgtgaatcct gaaaaagatt gtctaatttt tctgctaaaa
    20161 gatttagagt atcgatttcg cctaatgaca tgtcaaagat tcattattct gttattaatt
    20221 actttggcga caaaaaatga aaagtttagg cttttgaaag tgctctttca atcatgtttt
    20281 tgtaggcttc ctttgatgct gcaccaactt gctgtgcgac aacttggccc ttgtttagaa
    20341 tcatcagggt cggaattgaa aagatattgt attttgccgc aagctcgttt gcctcatcga
    20401 catttacctt gacaaacaac accttgccgt cgtaatcttt tgcaagctct tctactactg
    20461 ggccgaccat cctacatggc ccacaccatt ctgcccaaaa gtctacaaag actggctttg
    20521 atgagtctat gacctgtgat tgccagtcct ttgcattaga gacatgagtt aatgacattt
    20581 tttaccaaaa atcctccagt cgaagatacc taaatacgtt aaggtgtgat ttagaaaaag
    20641 cagtatatac ttaaatgacg aatggtcatg atgcaaatta tgagctccga gctacgcgtc
    20701 aaaaagttga gaggctcagg cggttatgtc atggcccaag tctctgatga gcagcaaaga
    20761 aaaggaaacc ttggtggccc cgacttgttt ttggcaccaa ttggaagatt agagccacaa
    20821 ataatttcaa aatattactg taacacttgt gagaaggatt acgaaggaag tccaaagata
    20881 gagtacgaaa acccaaatga ggaagttgct caaaatctga ttttattgga acgtggacaa
    20941 tacctctgta ccatttgcgg ctctacgatc gcagaatatc gcgagtttca aaaaccaaat
    21001 caacaagcag atgttggact tgcaaaaccc attacacaga catttgagca gcccgctgtt
    21061 ttcgttccaa cagttcagcc aactattgaa ccagtcattg aaccacctat cgaattagag
    21121 gctccaacat tttcttcaac aacagatacc ccatcatcat ttagctcaat tgtggggatg
    21181 ttagtgtatg actctgatgc aaaaagaatg ggtgttgcaa aacaggtagg agtagactcg
    21241 gcacaccaag tagtgttgat tgtgacaaaa aacaacggtc aagatgagac aatagaatgg
    21301 ggtaggatca aaaaaattgg cgagattatt ctattagggg aggaaggaca gtctgcaagc
    21361 ttctcttcac agggtttgag atgcacaaaa tgtagctttg agaacaaatc aggctcaaag
    21421 ttttgtgaaa gctgcggaaa caagctgtaa gatggcaaaa tcaactgcaa agagtatcgt
    21481 aaaggatatc ataattgttg cagttggagt tgctgtaatc tggtttggac tccaagtaat
    21541 atttggaaca ccaaatccgt tttacgtagt atcaagtgga agtatgattc cagtcctaga
    21601 agtgtatgac gttttggtag ttgatggaaa agtgcccttc aatgacattc agactggcga
    21661 catcattgtc tttaatcgac caaatggaca cgacagagta attgtgcatc gggttgttgc
    21721 agtactagat gaagagccca aaacaattag aacgaaagga gatgcaaatc cagtctcaat
    21781 cccggggact gactatccca taaccaaaga cgaatacatt ggcaaagttg cctatgtcat
    21841 tcctcagatt ggttatgtaa caagaatact tactccccca atcaattaca tcattattgc
    21901 cgtaatcatt ggaataatga tagtaaagca gtactctaaa ggcaaaaagc acgagccaaa
    21961 gtttgatacc gagccacaaa aagaccccct cacagatagt gaggaatttc caaagcaaga
    22021 cgatatagta tacagtgatc ccaattccga cataactaaa aaagaagagc ccaaacctga
    22081 ttcaaacgta gaatctgatg taacattaga ttcgaataaa aaagacgaaa gcgatcagaa
    22141 caaaagccag tagatttact aaaattattt ttctgggatc ttaaagacac gcttaaagta
    22201 atccgaattt tctggtgatt cttctgcatc accttttacg ccagcaagat tctttgcaag
    22261 gtttttcttg tagccaagct gcatctgacg cgctatttgg attctttggg cttgtggaga
    22321 tcctgctccg tgcatcgatt ctgtgaggta tcccactgca tttcttccaa gtgtcatgtt
    22381 ttcaatcagt ctgagaattc tcattctgtt ttcaacgtca acaccctttc ttcctgccaa
    22441 gtattttttt agcagtggcc ccgttactgg atttttaaag tctttttctg atggcattgt
    22501 aaccatcaat ccgcctgcaa tgtcttgtgc aagtcttgaa atctcgtatg ggaacctcgt
    22561 tacgttgtgc ttgcagacat ttgccagcat gtcgtcattt atccatactc ctgacttcat
    22621 cttgtgtgcc tgatacgagg atgctatacc tgcggcgtag atggattcgt ttagatgagt
    22681 catctctacg agtttttctc taatgtgaga tacgtctggc acaccgttat aatcagcaat
    22741 tgaggctgct gctccaatca tcacatcacc aagacccgtc ttgcaaacgt aactacgtct
    22801 gtgataacag gtaaatcgct caattagcat tccggcaaat tctgattcgc cgttcgtgaa
    22861 gatcttgtcg tttggaataa agacattgtc taaaattatc attgcttctt gccctgagaa
    22921 ctgggcgttt cctgcatcaa tgtcaccacc ttccatgctt cgagtatcgc aagactgtct
    22981 gccatagatg taggtgattc caggtgcatc gactggtatt gcaccaacaa ttgcatagtc
    23041 tttgtcttta tctgttaagc gaatggttgg cattatgatt atccagtgcg aattgatgca
    23101 tccagtctgg tgcgcctttg cacctgtcac atagacgccc tcgtcagttc tctttacaat
    23161 gtgtacaaac atgtctggat ctgcttgctc aaatggcgcc ttgcccctgt ctcctttgac
    23221 atcggtcatt gcgccaccga tgacaaggtt ttcttcttgc attttcttga tgaattcaag
    23281 taatcgtttg tgatagctgg tatgatattt ttcgtcaatt tcaaaagtaa cagagtgaag
    23341 tgagtttagc gcatccattc cgacacatcg ctgaaaacat gttcccgtaa gctgtccaag
    23401 ctttctttgc attttgtttt gcaaaacaag atctgttgca gactcggcaa tgtgcaggaa
    23461 cctgtttaca cgttttccag aaatttttga aacgggtgct gccaagtctt cctctcgagc
    23521 agccaaatca taggtttcgg caactgcgtt aattgagggg cggatcattg gatgatctac
    23581 tggctctttt acgagctcgc caaaaaggta cactatcaga tttcttcccc ttaggctttg
    23641 gatgtattct tggcctgttc gaattgtcat acactaaatt ttgttcaaac tgttataaat
    23701 attcaatatt tattcaggaa atgatctaca tacttccttt aaaaatatgc taaaaatttg
    23761 aatgcccttc aaactaggct gagctaatca ggtttttggt tctcgtgttg aaaaattttc
    23821 tatatacaac agcaatgatt actgcgccta caaatggtgc agtccaataa agccacagat
    23881 cacttgtata tcctgataat atggctggtg cgagtgaacg tattgggttc atcgacgccc
    23941 cagaaataaa cgacagaaaa taaatatcaa aaaatatggt acttgcaaac atgacaattc
    24001 caaatcccct taggcctttt gtatggactg caacaagaat agcagaaatc aaaattgctg
    24061 ttgcgatagc ctcgattcca attattacat aaattggaaa ttcatagttt ggaaagtttg
    24121 cgccaagatt tgcctctgta ccaattacaa actttacaaa caagcttgct aaaaatgccc
    24181 caattagttc tgctccaaga taaagcggta aaagttttgc cggcatgtgt tttgttatca
    24241 aaaacgtcaa cgttactgcc ggattgaaat gagccatcga tgtttttgca aaaagcgtag
    24301 ccatgactgc gactgccaca aatggcgcaa acgcctcaaa ccaaagtcca atagtaccag
    24361 ctgactttac attcaaaaca actgaccctg tagcacaaac cactagaaca aatgtgccta
    24421 caagctctgc aaaaaagatt tttttatttg cagaaagttt tcttattgaa gcgttttgca
    24481 aagctcctga accttttttt caatcatatc acgaatatgt cttacttcat caagtgattt
    24541 tccttttggg tctggaattg accaatctac aacatcatta acaaataacg cagggcagga
    24601 ctctttatcc atacacccca tattgacaat tctggcagct tcctgtatca tagaatctga
    24661 aattgttttg ggtttttgag aggcaatgtc gattccaatt tccttcattg cctcaacagc
    24721 aaatggattc acctcattgg ttggctgtgt gcctgcactt tgtggttcat atccaggtgg
    24781 acagtatttt cgaaagaatc cctctgccat ctgacttctg cctgcatttt caacgcatac
    24841 aaataggatt ttcttatttt gtggcattaa aaacttatat aaatattagt ttatataaat
    24901 tatgattgat atgaatggaa caaccctgct caaatgcatt tgtgaggata cacgctttag
    24961 catactggag cttttgcaaa aaaacagtga gatgtcagta aacgacattg tctcaaagct
    25021 aaaaaaagac caaccgttaa tctcacatca cctgcgagca ctcaaagaat gcaacattgt
    25081 caagacccga gaaaatggaa agatgacaat gtattcagtt gcaaacaagg atctctcaag
    25141 actgataagt gacatcataa agacatcaga aaagatcgca tcttgttgcg aatccactac
    25201 atgttactaa atttgaaaaa tttattctga tttgagatgt tttaaggctt tttttgcctt
    25261 gtagatcatc atttttaaaa aaaagtgagc attcctacta gatagtaaga aatgccccaa
    25321 tgcctgcccc aacgccaatg cccaaaacga agaatagggt atcccaggca atgacttttt
    25381 tgaatgaggc acgcacctcg tagttccaat gacttggttt agggtcttgt accatgatga
    25441 aacaatttga caaaagaaat tgataggttc tagtaagcta gtttgttgat aaatcagcta
    25501 agttgttgat ttaatctaat atctattgtt tgtgaacgaa taaatgataa aaatgcgagt
    25561 cgatctatgt agaaaatgtg gaaacgagtt aagaaaattc aatcaggcag aaagatgcaa
    25621 tacatgtggc gaagaatttt cacagtttac ttgtgcaaga tgcgacatag taaccgaacc
    25681 gcagtaccat gtacacacag atagcaacac tacttttgaa aatccgataa catatctgta
    25741 caagagacga aattgattca aaaattacaa atcggtacag ttgggccatc tactctacca
    25801 aggcacaaga ttgggtttta tgcactgatt gttattacat cagctgtagc agccattctc
    25861 acaattagct aaaatcgaag ttttaggaga ggcctattct aacatctagg atcttgcagc
    25921 catttccttg ctccataaca aggactggat tcatatcaag ttcctttatt tccttaaagt
    25981 cagttacaag cgcagaaagt ttttgaataa gctctgaaag ttttttcaga tcggatggtt
    26041 tttcgccacg aactccttca aggagttttt ttgttttaat cgagtttatc atgtcgtctg
    26101 cttctttatc tgtgacaggt gcaagcctaa aggtaacgtc tttgagtacc tcaacataga
    26161 ttccacccat accaagcatt atcacaggtc caaaccctgg ttctagtttt gagcctatga
    26221 ttagttcctt tccgcccttt accatttcct gtactagtat tcccttgatt gtggcatctt
    26281 tgttgtattt tttggcattt gtaattattt gattaaaggc atcccttacg ccctgctcgg
    26341 agctgacacc gacttttacg ccgctggcat cggacttgtg tatgatttgt ggcgatgcaa
    26401 tcttcatcac cactggaaac ccaatctttt ttgcggtatt aactgcctca ctttctgtcg
    26461 ttgccaagat gctttgcggc agaggaaatc catatgccct gagtacctct tggccttcct
    26521 cctctaagag gtttgttctg ccttcttttt tgacagcatc aaagacgttt tttactttgg
    26581 atttgtcaac ttggaattct gcaatgattc cttccggggt cttgagccag ttggtgaatc
    26641 ttagcattgc gcgaagtgtt ctgattgcac cttctgcata ggtgtagtat ggcacgtctc
    26701 cggcagcaag aatctctctg tttttgattc cctcgtcaag acccatcagg cttgcaagca
    26761 ttgttttttt gtattttttt gacatcttta cgattacttc agcaagcttg tcgtagtcaa
    26821 gtgttgcaga aggtgtgcac attacaataa cagaacccac gtttttgtgt gcaaggacat
    26881 tttcaaggac gttgttgaat cggttaaaat ctgcatcacc tacaatatcc actggatttc
    26941 ttgagcttcc ccagggagga attactgcat cgatttttgg tcggatgtcc tcaatttttg
    27001 ccatctttat tccaaacttg gagcaggcat cagtagagat aattgcagga cctcctgcgt
    27061 ttgagacaat aaccaagtca ccctccattg gaagtggttg ctttgagaat gctgttgcgt
    27121 agtcaaatag ttcttccatg gtatctaccc gaattgctcc tgcttggtgt aatactgcat
    27181 cataaatttc atcagagccc attagggcac ctgtgtgaga catagcagcc cttgcgcctt
    27241 ctgggcttcg gcccgacttt aaaaccagaa ctggtttttt gagcttcttt gttacctgtt
    27301 tgcagactct aagaaactct tggccgtcac ccatgtcctc cagatacatt acaattacct
    27361 tggtttgctc gtgctcagct agcattttga gtacgtcgat ttcactcatg tctgccttgt
    27421 tgccaaggct cacaacggct gagaatccta ttccctgtgc acttgcatcc tctacaagtg
    27481 ctgcgcaaat tgcaccgctt tgtgacacca gtgctatttg cccggacttt ggtgtaattt
    27541 tgagaaaagt agagttcatc attgtatttg gatcaaggtt catcactcca agacagtttg
    27601 gtccaattag tctaattcca tattttttga caatatcttt tagctgttgt tctagctttc
    27661 ttccctcttc atttacctct ttgaagccag cagtgatcac aattactccc ctgatacctt
    27721 ttttgccgca ttcttccaga acggttggaa caacgtcgtt ttttgtaatc actactgcca
    27781 agtcgatttg ttctttggtg tctaaaaccg tcttgtatgc aggcatatca aagaccttgt
    27841 cacgtgtggg agaaattgga taaatttttc ctgtaaaccc cttcatgata tttgatgtga
    27901 tcgcacgtcc tatacttcct tccttgtcgg atgcgccaat tattgcaatt gattttggag
    27961 aaagaaatgg tgattcggcc atcttaacaa atttgcctca gtcaactttc cataataaag
    28021 ctattcatgg aaaatttgat ctctagcttc caagcatttt tcctgcaaga ttctgagctc
    28081 ttggaatcca aacaatcttg atgtttggaa atttgccgag aagtgaccat gcctgacggg
    28141 caagctcgcg cagctgttcg ctatttattg caaattcatg atttagctgg gatacggtat
    28201 tttttgaatc agaataaatt gtaatttggt cgcttgattg gcctagtttt tgtatggctg
    28261 caatgattgc cagatattct gcctgattgt ttgtgatatt ttgttttttc tcatagaacg
    28321 actcgccagt ttcttttaca aaataaccat atcctgcatt gtccccaccc gaaccatcca
    28381 cgtaaacact aagtgccatc tttgtacaat ctcgttatcc tcacacccac gtttacaact
    28441 ataagatata acacaattga aaccacgtgt gcagcaattg aggccagata tgcctcataa
    28501 tttattgtaa gaaaataata ctgtagcgac catcttgttg tgacgtaaac tagctcgccc
    28561 actccaagcg atgaaactat ttttacgaga tcttttttta gtctgggaga gtctagcttg
    28621 cctgattcaa gtctgtattt ttttctatta tcaaaataaa acaaggtgat aaatacagaa
    28681 taaaatgttg tcatgtcgac tgtcactgta taactactgt tgatgtagct ttcatgatca
    28741 gcaaaaagtt gggcagttat cgcagaaagt aaaactgcca caatggttga taaaatgaga
    28801 cttctattca gcttgaggta ttctctgtac cttttttgca caattttaat aaagttatcc
    28861 tacaaataaa ccaagttcaa tccattgtaa ttttcaaaaa gatcaatatc ccagcaagct
    28921 ctgcaatgtg cacaccaagt aggtatggaa atatttcatg tgagaacagc tgcgacattg
    28981 aaaaatatgc gtatgcgcct atcgtgttgt gcaaaaatag cattactgaa aacacaatca
    29041 tgccaagtgg cagctgtgct tttgtatttg catacattct tccaaataca aacatgagta
    29101 ttccaagtac gatcatattg gcaattgata caccggacag aactgcagaa gttgattcca
    29161 tttagaaaag acctcccctc atatcgctta tttacttttt tccagtttat ctatgatttc
    29221 gttaaatgag tccatgttta cctccagaaa gtttgatata aaaaaagtga cattgtattt
    29281 ttccccaagt ctagttatta tgttgttttt ttcaagaacg ccgatatgat gctgaattgc
    29341 cttgtaatct aagcctagtt ctttggccag ctgatttgca ttgagtggtt tttcttttat
    29401 tgccaaaacc agcctaagcc tatttaagcc gccccttgag cctgcaaata cgaaccacaa
    29461 aagacgctta gcgtatgggt cgttagccat tatgatggaa atttttgata atgctacaaa
    29521 aacgtatttg tgagcctact tgcataacgc aaaaacaata taacaaattt ttgttataat
    29581 atagtaccaa atgatgctaa actacgctta tccgttgtat aggccgccat ctgagtcaga
    29641 ttctctgata ttccaggtaa cgcttggatg ctcgtacaat ctgtgttcat tttgtgacat
    29701 gtacagatct aaagaatatg tagagcggtc ttgggacgag ataaaagctg aaattgatat
    29761 catggcaaaa tcccttcccc agacaaaaag aatttttctt gccgacggtg atgcacttaa
    29821 cttggcaaca gactatatgc aaaaaattgt caagtatttg tatgaaaagt ttccaaatct
    29881 agaacgcgtt tcgtgttatg caatgccaat gaatgttctc aaaaagacac atgaagaact
    29941 caaaacatta cgtgaagctg gactaaacat gttttattta ggaattgaaa gcggttctga
    30001 tattattctt aaaaaggtga caaagggtgc tactgccgca acaataattc gagcttgcaa
    30061 aaaagcaatg gagactggat acactctttc ctgcatgata attttgggac taggtggaaa
    30121 aacctattca aaagaacaca tcaaaggatc agccgaagta atcaatgctt catctccaca
    30181 ttatgttggt gcactaaccc tatatttgga aaatgggatt aaggaagagt ttctaactaa
    30241 attcggcgag ccgtttatcc cggtgaacga ttctgaggct ctagacgaac ttgaagacct
    30301 cgtaggacaa attgatgtta aaaatcaagt aatttttagg gcaaatcatg gctcaaatgc
    30361 atttaccatc aaaggaacgt ttccccaaga caagcaaatg atgcttgagc gaatcagttg
    30421 gatgaagcag catcctgaaa ccgcaagacc acaaggcctt agaggatttt aaatctccaa
    30481 taggccggaa atctcatttt taggatactt tacaggccat caattattgg tttgagatga
    30541 tcaggaagta ctggaagctc tgtgtggctt tcgttgtttt gtagaatctc agggcctatt
    30601 cttcgaacac gctgcgtgcc aattaacgtg tcaatgcttc tttgtgcgtc cttttctggc
    30661 aatacggttt ttagtttttc aagtaatgtt gcctcatttt cgtacttttg tattgcatat
    30721 tgaactaaaa tcagcttgtc gttaaacgat aaatcagtca tcggtttttt cctgtgtcac
    30781 gtataaaaaa tattcctagt tttttctaat tccctcaaat gtggaattta gaatttcttt
    30841 tcttacaatt cgaatcgctt catagattgt agtctttata tcgtcaaagt agttgcccac
    30901 aattctcaca accaacccag agttgtctgg aagaaaagaa catccgccaa ttacttcatt
    30961 gttattatca aacaaattgc ttatcttttc ttgtatgttt tgaacatgtt tttgtgttaa
    31021 aatgtaaatt gttccaaaaa tggtgtgtcc ttcgagcatt ccaagagatg agaatttttg
    31081 tttttgggga tttagcattg atgcatctgt aaacttcatc tctccttttt ggttttttcc
    31141 aatggttcta agataacaaa tgtcatagct gaacatctct cccattgcgg cacggccaga
    31201 gctaacgact tcagagtata ctacggttgc agaatcatgg gctgtgatat tgactttctg
    31261 atagtacctc gaatttttat atggaatgat ttggtctgga atgaattcga ggtaacaatt
    31321 ctctgccaca tctatgttga gtagctgagt tgcgtagttt gaatccattt tgtatattcg
    31381 ggtagctcct tgtgttgtaa tgttagcaag ggcgttgttc ttaagagcaa tatccattcg
    31441 gtatctatct ccctgaataa ttccacctga tgatgacatg atgaatagat aagccatggc
    31501 agggtttgcg ctatcataat gcaggacttt ttgcgtataa agtggaacct ttgagtattg
    31561 ttcggttaga atggttctgt tttttgttgg atctttttct aaaactattc ttagtatgcc
    31621 tgtctttcct ggagttccaa cgccaagcat ttctacatct tgatttaacg ctaaaaattc
    31681 aggcggaacg tcatttggtg tacaaaaatc cagatccgtc atctctgatg caacagggcc
    31741 tttggaggtt gttcaaatag aacgtctctg acaatgtgtt cagcaatttc tttgatgccc
    31801 gtgtcgtttt tgcagtttac aaatacaaat ggtttttctt ttctcatttt tttggcatca
    31861 tgttccatca tagaaaggtc ggccccaact tgtgacgcca agtcgatctt gtttatgacc
    31921 aaaagatcag atgactcgat accaagtcca ccctttcttg gatacttgtc gccacctgcc
    31981 acatcgatta tgtagataaa ataatctgca aggatgggac tgaatgttgt tgtgacatta
    32041 tccccgccac tttcaattat tatcagatca agttctttgt gatctctttc aatctcatca
    32101 attaatgcca gattcattga gggatcctca cgtattgcag tatgcggaca tccccccgtg
    32161 gctagaccaa ttacaagatt ttctggtaat agtttctgct gagttgcaag gttttttcgc
    32221 attctttccg catcttcttt tgaaataacg tcatttgata ttatacaaat tctatagcct
    32281 tgtttggcta agacgggaac aacttgttca atgagcgtgc tttttccaga accaaccggt
    32341 cctcctattc ctacccgaga gattctatca gacaatatgg aaaatttttc ttaatttagg
    32401 taataaacat tttagaatcc attttttcgt gatgaatttg tattatgtca agttgtggcg
    32461 caaactgcca catctgttcg tatggtactt tgaggtattt ttgcacagtt tcactgatcg
    32521 ttggttttag ttcatttagg attttttgac cctcaaagtg ttgcaaaatc cctaaacgga
    32581 gtgcagcgcc tatgacactc acggcaaatc cgtaaaagag catcaaggcc gccttttgct
    32641 ttggtatgcc aaataatttt gctgcaattg caatcgatac tggataggtt cctggggttt
    32701 ttgaagacga tattgcctgc agatacttat caaggagatc atctctgaga aatgacgcaa
    32761 ggcattttac gagttgtctg cctgagcgca ccgacgcatc cctttgttcc tttatcagtt
    32821 ttattttgta aatggattcg tcgacgccaa acagtgcatt tgtgtcgtta ttttcgacga
    32881 aatcatatgc gttggcaagt gccacacagt cagcaggtcc aatttgctgt agaaaataca
    32941 cctctacgaa attttgaact tgttctggag aagtaatgcg tttttgtaaa taaagtgatt
    33001 caaggccatt tgacatagta taaagaccgc ttggaaaaaa cgaatctgcc agttgcatca
    33061 gaccaaggtc tgcaaagtca tccatgctca tgtacatcca ttgtatcgtg tggttgaaag
    33121 actttttctt ctactgaaag ctcaattttc gcagtcatat ccttgaatag tgatttgaaa
    33181 agatcaaatt ccgactcgct gtaaattgga aatattattg atccatcttc atacgcaatg
    33241 ggcctatgcc tgttaccaat aatgtggccc agcattacaa aaagttccga atttttttcc
    33301 tcttttgagt ttaccctaat tactttttct ggaagctgct caactagtat ttttatggaa
    33361 tctattgaca atacatctcc gtggtgtaat cttattccag aggcaaggtc tataccaatg
    33421 tcggtaccat cttgcgtttt ttttcgaagc cgagtttttt ccatttcatg ccttgaaagt
    33481 accagtctct tgtagttctg gctttgggct tcaaactctt ttgcaagctt tgaatctcta
    33541 aagatattcc catagatcga atttgaaagc agcatacctt acaatatgga atccaatgtt
    33601 atatttttga agatgactca gtaataatgg tgcatgggtc gttggatctt tcccattgcg
    33661 tcgtgatcgt gtttttcact gcccccagcg tgtgaatggg tcgtgccatc ggcatgagtg
    33721 tgggtgttat caaggggttt tttttcaaaa atatcgtcaa ctcgaagcaa catttggacg
    33781 acttcggagg cggtctttag tatctgttct tttaccacca ttggttcaat aacatttttt
    33841 gttgaaagat cttgcacttt tctttctatt gcgtcaatcc cgtaccattt tgagatattt
    33901 tccagtgaga tctggtcaac tttggacctc aattggctgt aaacatcggt tacgctcatt
    33961 cctgaatttc ttgcaatcgt caatgggatc tcttctaagg cttcggcaaa cttggctact
    34021 actagttgct cctttccttc aattgtatat gcaacttgac gtattttttt tgatattaat
    34081 gcctctgttg agcctcctcc tacaacaatt tgcggtttga caataaagtc taccagaaca
    34141 tggattgcgt ctaaaacaga tctatgaaac tcgtcaagat accttttaga ttgcgatctt
    34201 aacaaaatgg tcactgcttt tgggttgatg catctctcaa cgaagaccat tttatcatcc
    34261 ccgacaaatt cttcgtaaac tttgcctgca aaacctaaca tgtctttaga aacttcgtcg
    34321 aggctaacga tttttgctcc agtagctttt gataaccaat gaagatcatt ttcttttact
    34381 ctgcgaaccg aggcgattcc agcctttagt agctgttctt gtgcgcgcaa gttaattcct
    34441 tttcttgaaa atacaacgtt tgctccagaa tccataatca ttgaaacttt gttttttatc
    34501 tcttctgatt ttttttgaag aaaaacttct ctttggccaa ccgaagtaat tataatttca
    34561 gactcggttt tggtagtttg gttatccagt tcatcatcta agagaaggat ttttgcgttc
    34621 tgaatatttt ttggcatttg agagttatca attgttttat cgaccaccat gcccctgatc
    34681 agagtcgttt tgtctgcact ccctggcttt tcttcaattt taatgtcatc atgttcaatc
    34741 ttgttatttt taaaatcaga aatagtcgat actgctttga caatcagctt tgcaattgta
    34801 tcttcatcag caatattgga aattgctttt gatctcaaac atgtcaatgc aaggttttct
    34861 aatacagccg catcagaatt attctttgtt ttagcaatat tgtacagagt ttcaagtgaa
    34921 atctccaagc ccctttcgta tccctgtata attactggtg ctgggattcc aaggtctaac
    34981 aattcttcgg ccttttggat taaagcccct atcaaaacag tcacagtaat agttccatcc
    35041 cctacagcat tatccaccga attggctgcc tcaattacgg ccttggcagc tggatgaaat
    35101 agatctactt tgcgtaggaa ggccgcccca tcttttgtta ctgttgcgtc gcctaaaaca
    35161 tcgatataca ttttttccaa gcctcttggt cctaacgagg ttttgactag gtctgccgtt
    35221 agcctggcac caagcagatt gtatcgtcgt gatttatcgc tctttgtata acgtagatcc
    35281 tcaggaatga ttttttccag atttgccaat caactaaaac tcaaatggag actattaaat
    35341 ttacagaatc tcactaatgt taaaaaaatt tttttaaatc aaaattattc aaaaaattgt
    35401 tgtaaagaat tttgattgcg aatgtacgtt ttaatcaaat atttcgtaca agatgtttta
    35461 ttcgcacatt acggatagtg ggtttggaaa aaagtcaagg acaatatatt tttaaattgt
    35521 ttgaggtata gttgatcatg ggttctgtta caacaaaaga cgacatacga agagaatcag
    35581 agagagtaat taaggccctc tatggtaatg gagttgcaga tcttgccatc agggaggtct
    35641 ttgcgctacc agaaaaagga ccacgcactg catgggatgt gcaagttaca ttcatgttgg
    35701 aaaaactcaa gtacacagtt gatcttgaaa tgcaagaaaa aaacggccaa gtgacaaacg
    35761 ccagactaat tgacaccatg gtgccgcttt aggcgcgttt tgtttaattt tttaaaagtg
    35821 acatattcaa actgtcagta tctttttggc ttcttgaatt ttttccaata attgcttgag
    35881 gtacacgtca gcaaacctct caaaggtcat ggcgtcgtat ttttgtgcgt actccttttt
    35941 tcgttttttg tattctgatt gaagccaatt aaattcggat tcatttatcc gaataacata
    36001 tttctgagaa tccctctttg attcgaacat attgacaaga aaatagctaa gaaattgggt
    36061 aaaattttct attttgtatt tttctttgta ttcgtctttt aatattttgt aattgttttc
    36121 aagccatact ttgacatcag ttctaattgt gatggatgtg tacttgccat tcagatctat
    36181 ctttatgttg tgcggctcta cggtgtaata ccccaatttt atttcgttca tcaatattat
    36241 caacgagctg ggaagaaaca ttcctgggta atggtcacca gtgatttttt ttaatcgtgt
    36301 caagacatcc ggctttaagc caattgttcc atatcctact ccaggcattc tgtataatgg
    36361 ctaaaaagta gtaattataa ctttgtcaag tgacgttgta gatctacttt tttttgtatt
    36421 ctttgattat tctaaaaatt atccatactg acgccatgat aatagtgcct attcccaacc
    36481 caacgtagaa aggacttgtt cctccctcta actgagttaa gcccaagcca gctaagataa
    36541 ttattgttgc aaagatatct ttcttatcca ttagtggatt attttaaaaa ttagtgcact
    36601 aataagactt ctctggtagc cttggtgacc acaccgtttg aaacacttcc caataatttt
    36661 gagccccaat ctgccctgcc gagaactaga agatcaaact ctttttcatg ttcaagtatt
    36721 gcttctcgtg gttgccctct tgagataatt ttactaaatg taactccatg ttcttcacat
    36781 tttttcttgg ccatatccaa atatttttca gcattttcct tttgttgctt tctccacggc
    36841 tgttctagtg ctgtaacaaa tgatcttgga taaggagcca gaacgtgaat tccggtaatt
    36901 gaagcgccaa ggcccttcgc tattggaatt gcgatgttaa gacatctgtt taagcttgat
    36961 tcatctccaa ttgcaacaag aatctttttc atggatttcg tagatctggg atccactatc
    37021 ttaaatgtaa atgttatttt tttcccagat aaagcctcag tttgcttgat accactaaga
    37081 tcagtttttt caattttagg tcatgtaatt gtagaataca tatgtgattt tggtcacaat
    37141 atcgtacatt acttgaattt tctaatcttt aaataattta cagtgattgt tagttgggca
    37201 atgggagaaa tcactgtcgt gggttgggcg atcattggat tcctaattgt aacaatagta
    37261 gtgggaacta tgacttatcg ccttgtgcag agaagcggca ggagattgat gattgccgga
    37321 aaaagccttc ctctatttat ggtcggcacc atgcttgcag cacaatccat tgacggtaat
    37381 tcatccctag gaaatgttgc actagtttat gattttggat tttgggcagg cgccgtaatt
    37441 ccaataggcc ttgggatttg tttgctcatg acaggcttgt tctatggaaa aattttgaac
    37501 aaaatgtcca tgtttacttt gccagacttt tactttagaa ggtacggaaa tggagcagaa
    37561 ggcatatcag gcgtacttat gataattagt tttaccgtct tggtggcagg taattttgcg
    37621 gcaagtgggt ttattcttga aacagttttc ggaattagtt atttgtgggc cattctaatt
    37681 gcagcagtta ttgttttggc atatacattt gcaggcgggt tgttttcttc tgcatataca
    37741 gatcttttcc aaatttatct ggcaatagga gcattctggg ctggattttt gttctttgct
    37801 ggcggattct caggcaccga gttctctacc attcttgatg ccaccccagc tggctacttg
    37861 gatttgtctg gcctgtacga tgtcgcaaac ggagcattac ttaactgggc tgcaattttg
    37921 gcactcggat taggagatat tgtcgcgttg gactttatgg aacgagtgtt tgcagcaaaa
    37981 acccccaaga ctgcacaacg tggcgcattt atgggtgcag cactgacatt ctttacagtt
    38041 gtgccaacaa gcatgttggg aattattgct gcattttatc ttccaggctt ggaggatcca
    38101 aacctcgcct tgccagagct tgcactcaat cacatgccaa ttgcaattgg agctgctata
    38161 ctaatggggg tattgggggc ttcaatgtca actgccaacg gaggattatt ggcaatatca
    38221 agtgttgttt cacgaaactt gattcaaaga ataattcgaa gacgttggtt gggtcgagag
    38281 gcttggactg acaaaaagct tctgtggatt actcgtgcaa tcaccatccc catgatggta
    38341 gccgggttgg tgcttggtta ttttgtacca gcccctggca tctatctaat cttggcattt
    38401 gatattgtgt ttgctggagc atttgcgcct cttacattag gtctattttg gaaaaaggca
    38461 aacatgccag ccgccattgc atcactgtta tttggatcgg gtcttaggct cttcttcttc
    38521 tttgcatttg atcaaggact tatagatgca gaatgggctg gcttggacac attgattccc
    38581 ccacccatct cctttgcttt attcataggt gttgcgtatg ccacgcagaa aaggtatcca
    38641 ggcaaggcaa ggcacgatgt aagagattac gtgcccccag aagaagatct aatatctggt
    38701 gaagatctaa aacacttcaa agaagttgga gatgcaggcg gaaacatatc agatagaaga
    38761 gctatctgat ctctgacttc gcataatgag tatggtattg aaaaattgtt ggcaccacat
    38821 aaatcgggga ctagtatcct taagctacga taactacacc aaggcagtat cagaatttga
    38881 taaagcaata aagatagagt ccgataacat ctcggcacat ctccataagg gaattgtgtt
    38941 atacaagatg cacaaatacg aagatgcaat tggttgtttt aacaaggtta tagaaaagga
    39001 tccaaaagat attgctgccc tgtatcatct gggtcgatgt ttggagggaa ttggtaaata
    39061 tgaaagcgcc atttcgtgtt ttgagaaaat tctaaaaata gaatcacagt acgaggaggc
    39121 attaatctac aaagggctca acattagtaa tcttggcagg catgttgaat ctatcttttg
    39181 ttttgatgaa attttgaaaa aggatccaca taattctagt gcacttgcaa aaaaaggact
    39241 ggtcttaaac agaatagaca aaaaagatga ggcgctgttt tatctcaaca aatcagctga
    39301 agaagattct aaaaattcac ttaagatgtt taaaaatggg tttgagctta ccagggaggg
    39361 aaaacacgag gaggccttga agcattttaa caaggcccta gaacttgatc ctaaaaatgt
    39421 taaagcacta gtccaaaaag gcataatttt tgatattgca ggtttttcaa gaaaagagat
    39481 agttgcattc tttgatagag cgttagaagt tgatcccaaa tgcattgatg cattgttaaa
    39541 caaaggattt attctagata gaactggatt ttttgaaaag gccttaaagt gttttgacaa
    39601 ggttttagaa attgaccctg ttaatattag agcaacatac agtaaaggaa tatccttatt
    39661 ttctttaaag aagaatgcgg aggccatgtt ttattgtaaa agggccatag agctggagcc
    39721 atcaaaccta aaggcgttgg gaagtatggt ttggcttcta gaacatatga aaaagtacga
    39781 agaggcagta gagtattgtg ataaggccat catgttagag ccaaattacg tatatgcact
    39841 aaactacaaa ggcaagatcc tatacaattt ggggaaatat gtggaggccc tcaagtatta
    39901 tgacgaggtt ttaagaatcg atccaattaa ccacagagca atctactaca aaaacgacat
    39961 catcgctaaa acttcgaaaa agaaaagatt tggttttatt gcaaagctaa aaaactaaaa
    40021 ttactttaca attttagttt ttgtctgtga ataaaatgaa aattaatgct gtttcataat
    40081 caaattgaat cgtatattac ggatattcca aaaaaagaaa ttagttattc ggaaaaaaaa
    40141 ctaaaaaaga aaaaaataac gctggtaaac tacttagaca tatttaatta cattctgcgt
    40201 tgtagtaatt tgatcaaaaa tgggaatgga gttttatgga gaatcataca ggggcaacga
    40261 tgaaccaatt tttgttggca taccaacatt cttgaaatta ccttggctca gaagtaaagc
    40321 tgaattagag aaagtaaaac cagatgttgc cataattggc gaaccgtttg actttggcac
    40381 tactattcgt cctggtgcac gatatggacc gagagctatt cgtgccgcat ctacagtacc
    40441 atctcctcca tatgaacact ttaacataga aaccggcgtc gacccctttg gagttttcag
    40501 aactatagat tacggagatg ttaatgtctc gccaggagac gttgtagaat ctcatcgaag
    40561 aatgacagac aaagtaaaag aggttttaga catagacgga attccagtca tgttgggagg
    40621 agatcattcg atcacgtttg caaatgtcag ggcatttgca aaaaaataca aaaacattgg
    40681 aatgattcac attgataccc atgcggattg tgctcctcaa ggcctttgtg gcttcaaata
    40741 cgaccatggt gcccacatcc gaagaataat ggaattaggc tgccttaaag ggaaaaacta
    40801 cacactcatc ggccccagag gatattggcc tggaccagac ttgtacaaat ggatggcaga
    40861 ccaagacttt caatggttta ccatgctgga cgtagaggag ataggaattg acaaaattgc
    40921 aaaggaagca gctgatagag ccaacgacaa cgttgatgcc gtttacatta gttgggacat
    40981 agacacgttt gatcctgcct atgcccccgg cacaggagag ccagaaccca acggcctcac
    41041 atcaagggag ggaatgaagt taatgagatt gttatccacc tcatttgatc ctgacaagtt
    41101 tgcctttgat ttagttgagg tggcaccaaa ctacgacgta agtgatggca actcatacaa
    41161 cggtggcata acttcaggac ttgcaaacag actaatcgta gagttattgg cgggcttgtc
    41221 attaaccaag aaaggactca aggaaggaaa accagtgcgt cctaatttct atcgcggaac
    41281 aggccatacc tatagttttg gaaaagaaaa agcggagatt ccgaagaggg attgatacac
    41341 gttaaggtaa cggtaaaagg ggagccagat acctctccat ttacaagagt cttttcgtat
    41401 ccaaagaaaa cagatgaaga gattttcatg aattctgcat ccatgatcaa agaacgacta
    41461 gacaggaatc tcaaaatcaa cataaatgag gcgctaattg tttattcttc ttacattgtc
    41521 ttagaacttg gagaaaacaa atcggtagaa caaattcaaa aaaacgccca taggattttg
    41581 agttcagacc atgtcctcat tggcgttcct gaaactttga gagaaatgtc atttgaagta
    41641 aagcttgacg atagacccat ccaggtagtc attctcaaca cacccattcc ggtttcggat
    41701 tatgttttaa aatccatatg attcaaaaaa ggttttagta atgatagaaa atcttatcga
    41761 acaaatccca ggtgcgacac ccgtctttgt acttgttgcg ggttttgtaa taggacttgc
    41821 ccatgcgttt gaaccggatc atgttgcagc cgttgcaacg caagcaacca agtccagaca
    41881 aaatcgttct ttacctgttt ttaggcgaat aggtgttggt gcctttaagg gctctattct
    41941 tggagcatta tggggagcag gtcatacctc aacattgctg ttggtgagtc tgttaatctt
    42001 cgtgttttca ttaagcatac ctccccaagt ctttagcaat cttgaattaa tcgttggtat
    42061 gatgcttgtg tttcttggag cctcaattta cctcaataaa aaattactca gaaagaggca
    42121 tgagcatccg catgttcatg aagacggtat gattcacact catcagcatg atcataataa
    42181 ggaacatcgt catggacaca aatcttacat cattggttgt attcacggtc tagcaggtag
    42241 tggagccctt gttgtgctag ctctttcaac acttagtgat gtgcagataa ttttatcgtt
    42301 tgttcttgtt tttggtgtgg gttcaattct tggaatgatg atggttagtg gtgctattgg
    42361 cttgccattt gtcatatcca cccattcaga aaagattcac aagacacttc gttttgttgt
    42421 tggagcaatt agcataacca ttggttttga tattctgtac aatgtcactt tagaaagtgg
    42481 taatttcttt ggattacact tgtgattttt ttatcaaatg aaaataattg actaggaatt
    42541 tttaaaatta ataaatacaa gaatgccaaa ttagatatca tgtttttgac tccaaaagat
    42601 attgacaaat tacagatttt cttggctgct gaattggcac gacgaagaaa ggctcgcggc
    42661 ctcaaactta atcatcctga agcagtagca ttaatagctg accatatcct agagggcgca
    42721 agagatggca aatctgttgc agatttaatg agttccggaa aaaaagttct tacaaaagac
    42781 gatgtattac ccggagtagt tgatttgatt cataccattc aagtagaagc aacgtttgat
    42841 gatggaacaa aattggtcac agttcatgac ccaatagtgt gagacatatg ataccaggag
    42901 aatatctaat ctcagacaag ccaatcattg caaaccaagg aaaaaagacg gtgaaaatac
    42961 gagtggcaaa cactggagac aggccaatcc aagtgggctc tcatacgcac ttttttgagg
    43021 caaacaaggc tttagagttc ccacgtgccg aagcatatgg atttcaccta aatatcccat
    43081 ctggcacatc tgtgagattt gagccgggtg attcgaaaga tgttgagctt actgaatatg
    43141 gtggtaagaa aatcgtctgg gggtttagtg ggctagtaaa cggcccatta gatgaaaaaa
    43201 gaaaggaggc gctagagcgg gcaaaaaagg agggctttag gggggcatga cacttgaaat
    43261 atcaagaaag agatacgttg atctttttgg accgacagtt ggcgacaaaa tcagactggc
    43321 tgatacagac ctgattattg aagttgaaaa agatctgctc agatatggcg atgaggttgt
    43381 ttttggtgga gggaaaagtg caagggatgg tcttgcacag gcaagtggcg taaagcgtga
    43441 gcaatcactt gacttggtaa taacaaacac catagtaatg gatccagttc ttggaattct
    43501 aaaagcggac attggaatta gggatgggcg aattgttgga ataggtaagg caggaaatcc
    43561 aaatatcatg gatggtgtag atatgataat ttcgtcaaac accgaggtaa tttcaggtga
    43621 acataccata tgtaccccgg gcgcaataga ttcccacatt cattttattt cgccccaaca
    43681 gataattact gcaatatgtg gtggcaccac aactatgata ggaggcggaa ctggcccagc
    43741 tgatggaaca aacgcaacaa catgtacgcc aggcaaatgg aatatccata gaatgattga
    43801 ggccgtcgaa gactttccac taaactttgg ctttttggga aaaggtaatg attcgcagga
    43861 aacggctctt cttgatcaga taagagaagg ggcttgcgga ttaaagcttc acgaagactg
    43921 gggaactaca cccgctacaa ttgatgccgc gctcagagtt gctgataaaa ctgacacgca
    43981 ggttgcaatt cacacagaca cactaaatga gtgtggatat gttgatgata caatagcggc
    44041 gattgctgga agaacaatcc atacgtatca tactgagggc gctggtggag gccacgcacc
    44101 cgacattatg aagattgcag gcgaaccaaa cgtacttccc tcttcaacaa atccaacaag
    44161 gccttttact gtcaacactc tagatgagca tctagatatg atgatggtct gccatcatct
    44221 caatccatct gttccagagg acgtctcctt tgctgaatca agaatacgtg ctgaaaccat
    44281 tgcagcagaa gacatacttc acgatattgg agctattagc atgatgtctt ctgacagcca
    44341 agccatggga agagttggtg aagtaacgat ccgaaattgg cagacagccg acaagatgaa
    44401 aaaaatagta ggaaggctaa aggaggaaac tggagataat gataacgtac gtgtaaagag
    44461 ataccttgcg aaaataacga tcaatccggc aatcactcat ggaatatcag aatatgttgg
    44521 ctcctttgaa tcagcaaaga ttgcagacct tgtgatttgg actcctcagt tttttggaat
    44581 aaaacccaag atgatcatca agggtggttt tatcgcatac tctttgatgg gggacccaaa
    44641 tgcctcaata ccaactacgg agccagtcta ctataggcca atgtttggtg catttggaag
    44701 ggcaaaaaaa tccatatcgt ttgtttttac gtcaaagcta gcaattgagg atggattaga
    44761 aaacaagcta aagacccgaa aaaagtttct tgcggtacaa aattgcagaa agattggtaa
    44821 aaaggacatg atacacaaca acataacccc aaaaatagag atagaccctg aaacatatca
    44881 ggtaacagtt gatgggaaga ttgctaccgt agagccagca actaagctgt cacttacaag
    44941 gctttacaat ttgttttaga tatttttgtg agctaagcag tttttgagcg tgtactgttc
    45001 atgatgtctt aacctgcatt agtcgctatc ttagactacg tgaatgagat cctttaggct
    45061 tcgccttggc ttgggatttc ttttttgtac tggatatcct atgcagagaa tcgacgatgg
    45121 cctgaggttt gttcctattg cttccattac cttttcctcg tcaatcattc caatccaaat
    45181 tgagcctagc cccaatgcag atgccgcaag ctgtgagtag gcacatgcaa gagtagcatc
    45241 ctggattgcg aattttttga gaacctcagg tggaaagttt agctttaccc tagagggatt
    45301 tttgcagaat gccaaaacta atggtgcgtt gacataaggt tggttgtttg ccgccggaac
    45361 tagcttttct tttatttttg gatcttttat gtaaaatacc tcaaatcctt ggaagtttcc
    45421 ggcagtgggt gcagtatctg cagctgccaa tattttatct attttccagt cctctattgg
    45481 ttttgttgca aattttcttg ttgaacgtct tgttgcaatg acatgaaaaa gatctgtatc
    45541 aagagattct tttgtttctg ctatcaaaat tgcactgaga agtttgtttt ttacatcctc
    45601 gatttgttga tgttgggttg gattgtattc tttgcatttg attatgactt tgccttcttg
    45661 ttcttcgata aacttgagaa acttttctgc aagtagtttt gttttgactg atagtgattg
    45721 tcctcccttg atgttaaagt cggtgttttc ctcatttaca atgattgagc ccgcgttatc
    45781 ctcaagtgtt attcttccca tagctttttg agaaagcatc gagtgaaggt ttgacagatc
    45841 atcctctgtc agatagatta ctttttcttt atcattgtct tgcggatcag cccatacaag
    45901 acgacagccg ttttcttttt ttatatacgg ataatctact ttggaaaatt cgtacatata
    45961 accaaaatgt aattaatgat attttaaggt cacaaaaaag gaaaaaatgg ccgtcctagg
    46021 ctgttgctag gaatgcgcca attccagcac ctacgccaat gcctaggacg aaaaagagtg
    46081 tgtcccaagc catgactttc ttaaatggtg ctcgaacctg atagttccag tggtctggtt
    46141 tggttattgt tttcataatt atactatacg gtggatattg atagggtctt gtcagctagg
    46201 atgatgaaat ctcagctaaa ttgttgaatt ggcaaagcca gtaaatcgct gtggtttaaa
    46261 caacaatcat ttttatgaat taatattgaa tttcttttat gacggctgta agaaagatca
    46321 cagaagcaga aaatagtcta accaagttgc aaaagttgtc ctcagataaa gaatttcagt
    46381 cagaattgaa caatttcata aaaaccatgc atgatgtgtt ttcacatctg ttagatgagt
    46441 acaacaataa atttgatttg aaaatagaaa aagttggatt aaacaacttc aaagctaagg
    46501 caaaaaagct gagcaaaata gatgccatga acttccttat ctggtacgaa aaagaatatc
    46561 aaaaaatcaa aagcgatgag atgtgtggct atcttttaga aaaaaaggac gatttgaatt
    46621 tcaatttttc aaatattaat aatgttatag ctgcttgctc tatgctctta gagaagacaa
    46681 gaacgatgac atattttgcg tatgaaaatt tctgaacaaa aaatacatga aagtgtgaaa
    46741 ggttgtccgt tctagattag caatgcagcc ccaaagactc cagcagagtc gccaagcttg
    46801 tttttcaaaa ttggtgtgtc gactagatca ctgaagactt ggccgtacac tgcgttacgt
    46861 ccttcagtat acaaaaaatc aacatttgat accccgcctc caagcacaat tacgtcagga
    46921 tccaaaatat caattacgtt tgcaagcccc ataccaaagt tttccagaaa ttcttttttc
    46981 cactgctgtg cctcatccga gttgagattt tgtactattg atgttaatga ttttgttctt
    47041 cccgttaatt cgctccatct tttctcaagc gccggaccgc ttatgtatgt ctcaacacaa
    47101 ccaaacctgc cgcagtaaca cttgtttcca ttttggtgca acgtgtgatg gccccattct
    47161 cctgcgatgt atgtccgacc ccggtgtatt ttgccatcga ttattattcc tcccccaact
    47221 cctgtgccca atatcacacc aaaaacgacc ccatagccta ccgcagcgcc agttgttgcc
    47281 tcagccatgg caaaacaatt tgcgtcgttt tccattgtga tttttttccc aagtgatttc
    47341 tcaaggtcgt cttttagtgg cattccgata agacattgag tattgctgtt tttgataaga
    47401 ccagtctttt ttgagattgt cccaggtgta caaattccca ctgaataatt tgagatgtcc
    47461 tttgtaagat cttttgttaa agagataatt gattctacca tagaatgata gccattttgc
    47521 tggtttgtag gaatgcgttt tctttgaatt atctgaagat tgtcatcaag gcagagtcct
    47581 tcaatttttg ttccacccaa gtcaattcct attttgtaca cgaatttcta atattgaaat
    47641 ctgttttgaa gttttttcta aaaactgatg ctatctgtga gcggcgccgc caggtcctgc
    47701 ggaaactgca atcacatcat caattcttag aatcatgcat gccgcttctg ttgcagattt
    47761 tgcgatctgt tcttttactg atacaggctc gacgatattt agtaagaaca tgtcgtcaat
    47821 ttttccttct tttgcgttaa ttcctgccca ttttctgccg ctgttttgtt ttgctctgag
    47881 atttgccatt gtatcaattg gattcattcc tgcattttct gctattgtaa gtggaattac
    47941 ttcaagggct tcggcgtatt ttcgaatagc tagttgttct cttccctcaa agctgtcaga
    48001 ccactccttt agttgagagg ccaaatatgc ttctggggct ccaccgcctg caacaattgc
    48061 tggtttttca atgacatctt ttactaccat caacgcatca tgaagtgaac gatcaacctc
    48121 atcgactact cgttgagacc cgccacgaat caaaattgag acagattttg gatttttgca
    48181 tccttcgata aagacccact tatcagattc tacttttctt tgctgtacaa gatctgcatg
    48241 gccaaggtct ttttctgtca tgtcatcaag atttgttgtg atacgaccgc ctgttgcttt
    48301 tgcaagcttg gtcatgtcac tttcttttac acgtcttact gtcaaaattc catattttga
    48361 taaatagtgt tgtgcaatgt catcaattcc tttttgacaa attaggacgt ttgcgccaac
    48421 tttttgtagc ttgtcaacca tagacttgag cattctgttt tcttcttcaa gaaacatttg
    48481 catctgagtt gggtcggtaa ttctaatttc agcactcatc tcagtttttt ctacttctag
    48541 tgctgcattt agtaaagcaa tctttgcttg ttctattttt gttggcatcc cactgtgaac
    48601 gacttctttg tcaagaacta ttcctttgat aaggtcagta tcttgaattg acgcacctgc
    48661 ctttttttct actttgatat tatcaagatc aactacatac ttgtcgcctt tcttttctgc
    48721 cacacgtaaa actgcatcta caacaagttt tgcaagaatg ctgctgtctt ctgaaattag
    48781 tttagactgc atgctagttg ttgcaatctt tagcaatgat tcgtggtctg ttggttcgac
    48841 ttttttggca atttgtgaca aaagctccat tgccttttct tgtgctgcct gatagccctc
    48901 gataataaca gaagagtgaa cgttttttgc caaaagctct tctgcttttt ctaaaagagc
    48961 acctgcaaag ataacagatg atgttgtgcc atctccaacc tcattgtcca cggttttaga
    49021 aatttccacc atcatttttg ctgctgggtg ctggacatcg atttctttga gaattgtcgc
    49081 accatcgttt gtaattgtta catcaccaat agaatcaaca agcattttgt ctaggcctct
    49141 aggtcccagg cttgtgcgca ctagatcggc tacaagcttt gctgccataa tgttgttttt
    49201 ttgtgcatcc cggccttttt gctggagtgc actctccttt aagactaaaa ctggaccatt
    49261 tggggtttgt tgaattgaag ccatgttttc ttgaaaaaaa tctttcaccc ccttttttaa
    49321 aagtttgcta ggctaggctc ggaataaacg atcttttctt tacgtctacg ataccgctgt
    49381 aaagaattct aactgaagga agcgccaaaa agggcaaaaa gagcggcaag aggtgcggtc
    49441 tttggaatct gcagccgcta tctgtcaaag tcgagttcag atgaacaaac ttttgtaaaa
    49501 catcatcaaa ttgatttgac gatatgattc cagctacttg taatggcaaa aatgacaaga
    49561 ttttaccatc ttttacgacc accagccctc cctgcgatct agcaagatgg tttgttgcct
    49621 gtgccatgtc tttttcattt gagccaatca ctatcatgtc attttcgtga aagcaccaag
    49681 tggatgcata cgcacccacg tttgcaccaa agtttttgag aaatccaacc gtgtgttttc
    49741 cagaaccgta cgtcctatca aaggcagcta ccttccagat gtccttatcg gtagatgaga
    49801 caacattacc tgcttttacg acaagctcgg ctgtgttttg ttttgtaatg atttctgttt
    49861 ccatttcaat aatatttgcc agaacagttg ggtttttact ttttattaca aagtcttttt
    49921 ctgtaattgg ttttgcaagc tttactgtct ttgtaatcca ttttggcaaa ggtgccttgc
    49981 ggatttttgt tacaatgctt ccgttggaga ctacgagctg ccctccaaca aagactttct
    50041 ttggttttat cttttccaag tcatcaaaga ctagcatgtc tgcaagcttg ccaggcgcta
    50101 ttccaccaag gtctttgccc atattataat aatcaaagca gttttttgat gccattgtta
    50161 tggcatctat cacatctacc cccaagcgca ttgattctcg tatgcaatgg tctatgtggc
    50221 catacttggt aatatcaatg gggtctaggc cgtctgagca gaacattaat cggttcaggt
    50281 aaggctttgt ggccagaact tgtggtatga tgtttttgag gtctcgccta attgagccct
    50341 ctctcatcat aatccacatg ccaagtctta gtctttccat tacttgatca tagtcaattg
    50401 gttcatgaca agaaagaatt ccagaagaaa tgtaggcgtt taattttttg tctgatgcgc
    50461 ccgcggtatg tccgtttatg atgcagtcat tttgtaacat gtttgaaagt tttttcatgg
    50521 tttgtggatc tcttgtagtt actttggtcc aagaaaagac ttcacctagt cctaccaccc
    50581 catcaagatt gattgcggat ttttgttctg caagactcag tgtttttccg tggctaaact
    50641 ttctgtctac tggtagaccg cccggtacaa catgaaatat tctaattggt aaaccagacg
    50701 aggccttgac aaattctcta aaccccctga atccacatac acttacgata tcaattggat
    50761 cagaaaacag acttgttgtt ccacaaagca aagaatgttt tgcaagctcc gcaggcatta
    50821 caaactggtc tatgtgcatg tgcggatctg caaaaccagg tgagagatac ttgttttcaa
    50881 ggtcgattac tgcagtgctg gtgttgactg tatgggaagc gtctgaacca acatatgcaa
    50941 ttctgtcctt tactatagcg acctgggcat ttggaagaat ctcctttgtg tagacattaa
    51001 caagcgaaca gttcttgaga accaaattgg cctttttttc tcccattgca actgcgttca
    51061 aaatagaaat catcgaggcc agctcagaag acatggtatc aatctacagc cgactttgtt
    51121 ataaattgaa agtgatgctt aaattaccat ccaaggcgtt gtctgctatg aacgtaccta
    51181 aggaaattaa tcggtactgc ccaaagtgca aaactcacac cttgcagaag gtttcactct
    51241 acaaagcagg aaaaagaaga ggttctgcaa taggtgaacg taggcacgaa gaagacaaaa
    51301 aagggtacgg aggacagaaa ttcccaaagc ttgccaagcc tgcaaagact acaaaaaagc
    51361 agactttgat tttcgcatgt cctcaatgca aaaagaaatc taataaaaag ggcattagac
    51421 taagaaagct tgagttggtt gcacattgaa aagatctcac atactcattc caacaccaaa
    51481 aagtaagttt cagaaggtgc agtgcaaaga gtgcagtgct gaaaacatct tgttctcaca
    51541 tgtcactacg attgttacct gcaaatcatg cggaaacgtg attgcagagc caaccggatc
    51601 cattgcaaaa atttacggta ccgtatcagg cgttcttgag taagtcatat tcgtgttttg
    51661 tgtttagatt taatgcaatt cttttgtcat caagtatagt gtaactttcc tctacttttt
    51721 gggaaatttt ttgtggattt attattgaga tccccgtgta atagcagtgc ttaccttcaa
    51781 aaaatacaga aaaattggat ctgagattta gcagatcaag aaggcttttt gttacaagaa
    51841 agctttgcca tgttgcatcc tttttgtgag ctgatatgat ctgttttaca atttgtgcgt
    51901 ctaaaagtgg aaggtcgcct gataccacaa gagctggctc atcaagtttg gagagtacaa
    51961 aattcagatc agaaacatac ccactcccag ttgttttgat tatttggatt ccaaggttcg
    52021 ataatagatc gtttgtcttt ggtgagttgt cacttgttgc ggcaataatt tttgaaaaac
    52081 actctgattc ttttagcgca tcaattacat gtaaaattac tggctttttg tatttgagta
    52141 gtaatttttc ttctggcaga cccattcttg ttcctttgcc gcccgccatt acaagtccga
    52201 tcatatcgac gcaaatacaa gaagagatgc taccctagtt atctcgtttg tcgcacccat
    52261 aatatcgcct gaaataccac caaaactgcg cctagacacc accaaaatta tcaaagttag
    52321 gactattccc aaaccaagtg ccaaaaaccc gacgttgttt tgtaaaacca caatagcgcc
    52381 gacggtaatt actgctgcaa ttacaagttt ttttcgattc ttcatgctat ccacaaaagg
    52441 cgagttagag ccttcccagg cagacggacc aaagcttgtc aaaagaacca ttgagaattt
    52501 ggcaattatt tctgcagcta aaattgctac aaagatctca aatcccttaa ttgttgagag
    52561 tgcaatcact gtgcctgcca catacaacac aattgctaca attcctgcag acccaacaga
    52621 cggatcacgc attacttcaa gttttttttg ctttgttccg cgaaccatca aaccatctgc
    52681 aaaatccgag agaccatcag tgtggtgaat gccagtaatc aaaacaaggg ctgccgtaat
    52741 taacaatcca acaattaatg gttccaaaaa taatgataaa ccgtatgcag tagagcctat
    52801 catcaaacct attgcaattc caattattgg gaaaagatac atgtgtcttg caacggtttg
    52861 cagatccgaa tttccagttg gaattattgt cagaaaagaa aagacagaac ctacttgtct
    52921 aaacatgaat ccaccatcca agataataca gaattgatat cataggaatt gttactagta
    52981 aacaaaacaa aagacaggtt accttcatta gcaaaatagc tgatctgaaa tgattctgat
    53041 ctagctctgt tgttccgttg cctaaactat aatggttgat tttttcaaat cttgtcccta
    53101 gtgctcctgc cattgcagcc attggatatc ctgcgtttgg gctctgagtt tttctactgt
    53161 cttgtcgcat cgtattcaac gcgtttttcc agtctgcgcc aattatgact gctgcaagta
    53221 tcataacata tgcggtaatt cttgcaggca ggtaattgag gattttatcg cagtttgcac
    53281 caaaccatcc aacattgcta aaaattgtat tcttgtagcc aatcatagag tcaatcgtat
    53341 ttaccgtcct gtagacgaat gcgcctggca agccaaagat gccaaagtag aacatcgagc
    53401 ctgttattcc atcaacgatg ttttcactcg tactttcaag cacgcctgaa acaacatgat
    53461 tcctatcaag atccttcgta tctcgcttga caagcatgga aagtcgttgt cttgcctcct
    53521 caagattgtt tcttgatagc gcttccatga caagtgcccc atgcctttca agccccctaa
    53581 tggcaatcgt ggatttcaaa agcaatgagc caatcagaat tataattatt gttgatatga
    53641 tatcagtcat gttttttgtg aaaaactcgt atgttaccaa tactgtggca ataattgcag
    53701 taacacacaa tgtaacgcca attccaccta gtttttctac agttgggttc ttgtcttttg
    53761 caagcggtac aagtcttgca actagcgccc caacccaagc agttggatgg agtttgtttt
    53821 ttggatcgcc aaatgcaaag tcaagtgcta gtgctccaac tgcaatcaaa attggctcaa
    53881 ggatcattgc agtaacccct caattgtttt tatgtcaatg tgtgatttta ctgtctttgc
    53941 aagtttttca atttctctat ccatcttttt ttgattttgc tttctgaaaa gatgaatttt
    54001 gttatcaagt gaatcctcgt ttggcagatc catatcgatt agtggtatta cgcctaaaac
    54061 agatttagat gttatttgct tgagccttct gaatcccggt ttgagtattt ttacatctcc
    54121 tcggaacttg tttatcacaa accctttgac tagtttttgg tgttgttttg gtaataatga
    54181 cattgttcca acaatgcttg caaatgtgcc acctttttca atgtctgcta caagaataac
    54241 agacgagcct gtcttttgag caatcctcat gtttgcaatg tcgtattttt gcagattgat
    54301 ttcagcgggc gagcctgcgc cctctattat tattagatcg tgattttttt tgagctgtgc
    54361 aaatgaatca agagctagac tcaggccttt ttttaggaca aattttttgt agtatgtttg
    54421 cgcgcccatt tccttgtaga atttgccttg tagtataact gagcttcgat agtttccaag
    54481 cggttttagt aaaattggat tgatgtgtgg ggaaatctca gtccgtgctg caattgcttg
    54541 gattgcttga gcctgcgata tctcaaatcc atctgctttg tatgagtaat ttgacatgtt
    54601 ttgcgatttg aatggagcaa cacgataacc agcgtttgca aagatcctac acaaggcagc
    54661 aacaagtgtg gtctttccca ctccagatga cgtaccctga atcatcaacg tttgtgtcaa
    54721 gctagtttct ccaatgccct taccagcttt agattatctt tgtgtgtttt aactgcaatt
    54781 cgaatataat tttgagccag tcctctaaat gtgctgcaat ctctgatcag aatttttttt
    54841 tgcaatagtt ttttttgtaa acgttttgaa tctgtctttg ttttaattaa aatgaagtta
    54901 gtcgtagttt caaaacaaga aaacccatca agtcccgata tgtagttttt caaaaatgca
    54961 gactctgtct ttatcagttt tcgcgttttt gcaagaaagc ccttctcaga tagtgcagta
    55021 actgcggctt cttgcgccaa accactgacg ttccatggaa ttttgatttt gtctagaacg
    55081 gacacaaggt tcctgcttcc aactccatac cccacccgta gacctgcaag gccaaatgat
    55141 tttgttaggg atctgagaac aaacaggttg tcaagttttt ggatttggtc aattactgat
    55201 tgttgtggag tttgtgttaa ttccataaaa cattcatcga caaagaccat tgtgtttttg
    55261 gttttagcat attttgctat ttgtatgatg gatttttttg aaatcagtgc accagttgga
    55321 ttatttggat tgcatacaaa gattacacca tttgttggta tttttttcat aaatttggtg
    55381 agatctagtt caaggttcat ggttttgaaa aattgcagtt tccccccaca aagcctcact
    55441 gcggcgtcat attcaccaaa tgttggaatt ggaatcaaga caggagtgtg tttgccaata
    55501 attgcacgac aaaaattgta aattacttcg gttgcaccat ttccaacaac tagattacta
    55561 gtagggattc caaggtacga cgatagtcgt tttttcagct ttttggagtt atgatcggga
    55621 tagactggaa ttttttccat ccaagatcct agtaattttt tgaccgatgt tggatacccg
    55681 agtggattga cgtttgagct aaaatccatg attgtagggt gtggattttc aattgaatat
    55741 aggccaccat gtggaaccgg agtgtgtgtt gctatgtttt tggcaaactt cacaaactca
    55801 ccagtgcgag cacgggttta gttgttacgg ttttggactc taaagaatta ttaattgatc
    55861 gcacaaaaaa atcccttgtg aagaaaagag taggcatatt gggtgcaact ggagcagttg
    55921 gtcaggaatt catactttca cttgagaacc acccgtggtt tgaggtgaca cagcttgcgg
    55981 cttcggaaag atccgctggc aaaaagtaca ttgatgcaat aagggaccca gactctggaa
    56041 tagtcaagtg gcaagtaggt ggagacgtac cacggtatgt acgagatttg atggtagtcg
    56101 cagtagacca agtaaaggcc aatgagcttg acttggtatt ttctgcagta gagtccgatg
    56161 ctgcaagaga aatagagacc aagttcgcca aagacgttcc cgtaattagc acgtcttctg
    56221 cttatagata tgatgaagat gttccaattt tgattccggg aattaatgat gatcatgtgg
    56281 agcttattga aaagcaaagg aaaaaccaca actggaaggg ctttgttttg cctttgccaa
    56341 actgcacaac tactggcctt gcaatcacaa tgaagccctt gtatgacagc tatggcgcaa
    56401 agcgagttat catgacttcg atgcaggcaa tctctggcgc cggaaggtcg cctggagttt
    56461 ctgcacttga tgtcactgac aatcttattc catacattcc aaaagaagaa aacaaagtaa
    56521 gaatagaaac tgccaagatt ctgggtaagc ttaaagatgg aaaaattgag ccagcaaaca
    56581 tcaaggtaag ctgtacatgt acacgtgttc cggtaattga cggtcataca gagtctgtct
    56641 ttgttgaaac caccaaacct gccaaggcag atgatgtaaa acacacaatt gaagagtttt
    56701 cacatcatgt tagtgtgcaa ggacttccat ctgcaccaaa ggactatttg attgtgcatc
    56761 aagacccaac aaggcctcaa ccaagaattg acagggagct taacgggggc atgacaacat
    56821 ccattggaag gctagaagaa gataccatat ttggcaacgg cgtcaaatac gtattgtttt
    56881 cacacaacaa aaagatgggc tcagcaaaag gtgcagtgct tttggccgaa atgctttaca
    56941 aaaaaggaaa aatctagatt cctagatttt tttcaaattt ttcgatcata acgttattaa
    57001 acagaaaaga atttgacaaa gcaggtgttt taaacggtaa aaattgatga gcttgaccgt
    57061 accatattat ccgagctgag cgaagatgcg tcaatttcga ttccaaagct gtcagaaaaa
    57121 ataaaggtca atccttctgt tgtatatagc agaataaagc ggctcacaaa caaaaaactc
    57181 attgagcgat tcacaatcgt agtaaatgac aaggagctgg gatatggggt aaaggcactg
    57241 acaggaatca acatggatac aaagttccgc gataccatta ttgatgaatt attcaagata
    57301 aagggcgtgc gtgaaatatc cgaagtcact ggtcgttttg acataattgt gacaatgtat
    57361 gcgatgaatc ttgcagaaat gcacaagctc atctcagaag gaattggtag tattcaggga
    57421 gtcttggcct ctgaaagctt catagaaatg aagcgtcgca ccaagaccat gccttataac
    57481 cacgaggcat aagcgtgtta catttaattt tgtattatgt ttttgtgagg tagggaaaaa
    57541 tgatgcaaaa ggcacagact ggttctcgga tcaaaaccta ttatgagctc acaaagccaa
    57601 agatttggta ccttttggtt tttactgcct ttggggcagc aataactgcg gcaaatattt
    57661 acaatgttcc agtttcgcct gcaacgtggg ctctgatgct ttttggagtg gcagcaggat
    57721 ctgcatctgc aaacactctg acaaactatc acgacagaga cattgatgcg ataatggaga
    57781 gaaccaaagg tcgcccaatt ccttctggaa gaatcgcccc accaaaggcc agggattttg
    57841 gattggtttt ggctgcaatc tcaatggcgt gtgcatttgc aatatcatac actgcaggat
    57901 tttggaatgg agtttgggct ggaattttca tggcgtttgg ccttgtcaac aatgttgttg
    57961 tgtattcatt aacgctaaag cgcagaagca gaacaaacat agtacttggc ggtttttgtg
    58021 gcggcgcgcc tgtaatgatt ggttatgcgg cagtgacgct acaaggtttg tgggacttgg
    58081 gtcttgtcat ggcaggactt gtgttcattt ggattccaat gcacatttgg gcccttacat
    58141 tgcatttcaa agacgactac aacaaggtaa atgtaccaat gcttactgct gtccagtctg
    58201 aaaaaacatc agctagaatc attgcaattt caacactcgt aatggtgttg tttagtgttg
    58261 ttccgttctt tttgactacc aatacaggcg aacctgttat gcgagaaatt tacctataca
    58321 ctgcgattgc gtcaggagta ctaatgcttg tactttcatt ttgggtcatt gcaaaaccat
    58381 ctgaaaaggc atcgtggatg ctcttcaagt tttcaagtcc atacttggca gttttattca
    58441 tcgcgttgat gatcgattct tcactgcgat aagttctcag acaacttgat tcagattata
    58501 ccgaatttac tttaatgctt tttttaatac ttggttaata acagcagaaa agcttacaga
    58561 cgatcctgtc tttttaatga gatctgcctg catgtttctt agctttgatt ctatgtcttt
    58621 atccaatacg attgtatgtc ttttaatcaa ttactctcat aagaacagat gaatcattgt
    58681 ttttcaatct aactataaaa taccaagttt ggtaaatacg tagttttgaa aagatttccg
    58741 tagacactta ttttacagta aaagatgtgg tgtcaaacgc tacattgtta tattttgctt
    58801 taatggtgta cttgcctgct tgtgaatttg cgtctaacat gactggaaca gtgaactgac
    58861 ctttgttcgt tagtattccc tttagatcat aagaagactg atcgggtttt gttattgtca
    58921 agaaaacata ggtgcctgat ttgaagttct cgacttgtcc tgtgatttgc atgttaattg
    58981 tttggtgtga ctgtttttca aaggtatcag catttacgct cacaaagcta agcttttgag
    59041 gttcgggagc cgattcctcc acagtttgag tgggcgagct tgatgttact tggatttgca
    59101 ttattccgtt tcttattaga tattggattc cctgtacaaa gtccaaatca gaaattaggc
    59161 cagatgccca ccattttgcg ttgtttttta tccagtacgg gatttgttgt gtgttttgtg
    59221 ttccttgtgt tgtttgcgga accttgatta tttcgacatt aatcagatgc tgaattccta
    59281 agacaaagtc gttgtcttca atcattccat ctgcccacca atttgcgctg cttttgatcc
    59341 agctggggat ttctgtttgt tgagaaacgg atggtgattc attttgtgtt tcagttattg
    59401 gcgtgtcagc agattcctct tgctcctttc caaagatgta aagccataca attccggact
    59461 gtgacttgtc gttgagattt cctgaaatca tgatgtcgtt tttttgagat gtgatctgaa
    59521 tatcagcaat gttgtatgac cttgcagaga tactaattcc actttttaca tactcgggat
    59581 aatcgtataa actgccgacc ttttttgtaa atgcgccatt agctgtctct tgaggagtta
    59641 tctcttcgag ctggaaaatt cctccaatat tctcgataag ctgtgaggca agctccgtct
    59701 gaagcggtgt gatgatttga tgttcggaat ctgtgtatga ataatcaagt tcatcttgga
    59761 attctttatt acaagtatca tcacgccaag cactagatga ttttattgca tcaatcatga
    59821 gctggccgtg aagcagctcg tggtaaaata tcacaagatc ctcggctaat tttacttgtg
    59881 ggtctttttc tgtagtttgg agaactgcgt tagcgtttaa cataaagccg ttgtcacaat
    59941 ttatggtgag tttgcctgca gaaaatgtaa tactgcattt ccagacgtaa attccaccag
    60001 ttactgcgcc ttcatggctt gccaatgagt catcaaaatg agcaagtttg gtagtaattg
    60061 ttgcattgac aaaattattt gtatccgtac tatcacatgg aaacgaaata acgattggct
    60121 ccttaaatga ctttagagta tcacttacat ccttttttga tattgttaat ttttcaaatt
    60181 cagcaagaag tttattgtat aggtcttgtt cttgtgatgt tggtgttcca taattaccaa
    60241 aagctggata taggtttgca aatagcaaaa aaacgacagc tagtgcggct ttcctcaatt
    60301 aacccatttg atcccaacag aatacttaag acttgggatg gattcattag cctggcccga
    60361 cttcaaccat agttattagt tctctaaagt cccgtgttac agatagacct tccttagttg
    60421 ttagcacaat agaagctagg ttttgtgctt taggtgaaac ccagattgtt tttcctgcgc
    60481 cgaggtcaat gtgttgtatg tcccttcccc atgttgagtt ttagttctgc tagaggtaat
    60541 tttccagtat taatgattcg tacgtgcgat tgcaactagg tcttgaacct tgattgcatc
    60601 ttcatctttc atttagaaaa attccaattt gaacaggcag ttgcaggcac ggatttgcct
    60661 cattattaag taaaatggtt tgcaaaacca ataattacca actattacca aagatatata
    60721 tactagttat tggtcattaa aacctatgag tggaaaacaa taccaagatt caaagaaaaa
    60781 tcaggtacca atccattcag aacaaaaggt acaaaacaaa atgacacaaa caaacgaatc
    60841 acaaaaagac gtgttttcag tgtacaccaa aagctttgat aagctaaaga gcgcctttga
    60901 gagaacatct acacaatccc tgcaatctta cactacccta aaccaggaga ttctctcaac
    60961 atggagtagc ttcatccaca cagttgcatc aattcagcaa aactttgcaa acaaggtagg
    61021 agtcaatgca agtttgccag aaacatcagc aactgtaatt caggatgcta ccgatgcatt
    61081 tgtcaagaca gttgagacaa acagtaaagt tgtccaaaca gcccttgatg caacagtaca
    61141 aaacatcaaa acagtcaatc agaacacaaa tgcatttgct gaactaaacc aaaacattat
    61201 caactcttgg atcagtgcct ggaaaccacg aaactaatct cttttttctt tttttataat
    61261 tttaaagtta ataaaaaaat tagaagtttt atgatgtatt ttgtttgctg agttgtttta
    61321 gcgcttcttg tgacttggct tctgcgtctt ttgcaaagtt ttgccacata ttttgccagt
    61381 agtctgcaaa cgatttgaat gatgcaaaac cagattcttt ccataggtcg tcccaagcct
    61441 taatggacca cgaatatgga ttgtattgtt caaattgttt tgagatgttt tgccaaagtt
    61501 cgttgtattt tttcatctca tcaaaattag attgtcgtgt tgctgtttca aatccctgaa
    61561 gatatagctt aaaggcattt gttgcagctt cttgcattgt atcaaatgat tttacccaca
    61621 tttcaagtgc ttttgagtat gcattccaaa gatctgttgc gttaccatct tgtgagacac
    61681 gagttgattt ggattcttta tccattcttt cttattgtgt ctcattattc aaatagttgt
    61741 tggatggttc ttaaaaccag aaattggtat tagttggtta tttatgacac aatatgccta
    61801 aaatatgctt aaacggggtt cgtgtattta cagactagag tacattgcta cttcccaaac
    61861 ggttggggtt tgtgcaaaca aggaatactc ttcatatttg attgtagtat atgtgtcaag
    61921 aaattcgcca gtaaagacat cttgtaagaa ttttttgtct gattgaagtg catcaagtgc
    61981 ttcttttagt gtagatggca agaatcgaac gttatagttt ctgcgttttt caggagtcat
    62041 acgatacacg ctttgttcaa cagggctacc tggatctaat tttcttttaa ttccatcaag
    62101 acctgctagc actaatgcag cctctaagag gtagatgttt gaagtagcat cagggactcg
    62161 atattctaat cgttttttat ttttgtcatc atcaaagact ttgggaattc taattgcggc
    62221 agttctattg ctcaggcccc aacagatccc aactggcgcc tcaaatcctg gtacaagcct
    62281 tttgtaggaa tttgttgtcg gatttgatat tgcacataat gcttctgcat gatccagtat
    62341 tccaccgatg tagtatctgg cagtttggct aagtgcagta acttgatcat ttgggtcata
    62401 aagtaggttt tcattatttg acgaaaagct gtggtgaatg tgcattgctg aagcgttatc
    62461 accgtaaatt ggttttggca taaaggtagc tacttttccc atttgttttg cttttacttt
    62521 taccatgttt tttattgaaa tgaccgagtc tgccatggct aacatttcat tatgctgcac
    62581 attaatttca cattggcccg aggttgccac ttcatggtga tgtgcctcta cctgtattcc
    62641 gaaatattga tgcagatcat cgcaaatgtc ttttcttact atgtccatcg tgtcctttgg
    62701 ttttgccata taataaccac ctttgaggtc gatgcttgtg tcctcttgtg accatggcgc
    62761 ttccatagat tttattgaat aaccaattgt tccagaagat gaaatggcgt ttgtggtaga
    62821 agggatcatc tttagggaat caaatatgaa aaactctatt tctggtccga accttgcctc
    62881 atcaattcca aattttctta gctcgtctct tgttttggca agtattgacc tagaatcttt
    62941 gctaaatctt gagatggact gatctttttt gtcataaatg tcggcaaaca cgcaggcatt
    63001 ctgcctgttc ccattgtcgt aataaggagg tagaatccta aatgttgaaa tgtcaggcat
    63061 caacaacagg tcggaatcat cgactgactt gaagccctta atggagctcg catcaagctt
    63121 ttgcaggccg tttttaaaat gatcttcatt tagcgaatat gaaggaatgc tgagactgtg
    63181 aagctcgccg aaaatattta caaaccaaaa ttcaacaaaa tctacattgt tgtcttgaat
    63241 ttttttgaaa acattttcaa ttttagataa attatatacg ctcattttta tttttttaat
    63301 ttgttttctg caaacagaat cagattacca attattagaa gtagcaaagc tgtgttaaag
    63361 tcaataccat ttcctaggag gcctcgataa tacatctgtg gcaagtttat tgcatatcca
    63421 attaatgcaa cttctccaaa gcaagaaata gtaatgccag caaaaaatgc cagatcgcta
    63481 atccttagca aagagctctt cagattaggg ttagtttttt gcgctctgaa aacctgcatt
    63541 tccatcattt ttcattcaca atttttaaga aaagttcttt ttcccaatat gccacttgtc
    63601 tagtggtttt tcgtcttcaa acgtgtacat ctttgtattt ttaatcgaaa ccaagactgg
    63661 aatttttttg ggttgttcct ttttggtctg atctaacgtt tttttgacag cttcctttga
    63721 tgattttaga atttgtttca gttcatcttc gtaaaagaat ttcttgccag aaaatggctc
    63781 cagatcatca atccatattg ctttttcgtc gtattttgcc aaaaatggtc tttgcaccca
    63841 ttttggattt cgtccctgca agaatcttag ggcaatcgct tttttctttc ccacatctac
    63901 aactccaagt acttggattt taccaggatt tgctgacatg cttggacccc tgactgttct
    63961 ggcaaggcca cttacctttt ggtaagcgtc gcgaaagatt tcttgggcgc ggacaagagg
    64021 aattccaaag tattcttggg ctccagtatc tcttgtcaca aacatgtaat atggaataca
    64081 gccaagactg acttgttttt gccacatatc ggcccaagct tgtgggtcat catttatgtg
    64141 agcaagaact ggagattggg ttcgaatttg cgctccagtc tttcgtattc tgcgaatcgc
    64201 ttcttgtatt gctctagtgg aaagctcaat tggatgatta a
//
LOCUS       DASWKH010000053         7411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1619861, whole genome shotgun sequence.
ACCESSION   DASWKH010000053 DASWKH010000000
VERSION     DASWKH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619861"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGA92_04265"
     CDS             complement(<1..273)
                     /locus_tag="VGA92_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845659.1"
                     /translation="MNKTIFGATIVAFAVLSFGMMTNQAFAQYMGNVDPQGFTGSNTL
                     EETLALQKARVDYAAENPATGSGTPYLAADGVLGASAVAAAVFGGIA"
     gene            393..842
                     /locus_tag="VGA92_04270"
     CDS             393..842
                     /locus_tag="VGA92_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9845660.1"
                     /translation="MGRVHTHRHGQSHSTRPITQKSPSWLTTSPKEIEELVVKYSKDG
                     VPMSQIGIRLRDQHAIPLVRPIVKKSIKKILEDNGITPEIPEDLNNIVKKAVNLQKHL
                     KTHRSDNRNVRSLELIEAKVHRISTYYKRIGALPQNWKYKSVVAQLE"
     gene            842..2254
                     /locus_tag="VGA92_04275"
     CDS             842..2254
                     /locus_tag="VGA92_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX9845661.1"
                     /translation="MRLEEALSNFDHTISDCIKSGKEICVVTHIDCDGLTSGSIITKA
                     LIRRGAKVTARTATEMNGAVIKNMAADSREFHVITDLGGGFAKQLDEALGQNWFVLDH
                     HEVPKEEHDNDRVINAWKFGINGGTEICAGGMAYLAAKSLDKQNIDLAPIAVVSALGD
                     RQDQGERKSFTGKNAEIAEEAKKSGHLKIDLDILLVGRETRPLADALAFTSQPFIEGL
                     TWNRDACFSLISSAKIAAKEGGRLRTPSDLNEDEKRLLIEAITKFASGQNANQIMEEL
                     IGYTYTFPSEDRRNFLRDAREFSTMLNSCGRIGKSGVGIAICMGDRNKMLKEGETILV
                     EYRKMIREYMNVLTNERWRVNDSMNYLMVNGEGVIPETMTGTISSLLAGSPKNAGKII
                     ILRTNGEENTVKFSSRKSIGCKSGVNLSVMMRTGAEKFNGIGGGHDAAAGARITKDKL
                     DGFLDFLEDTVNGNQDSNQN"
     gene            2226..2462
                     /locus_tag="VGA92_04280"
     CDS             2226..2462
                     /locus_tag="VGA92_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HEX9845662.1"
                     /translation="METKIQIRINDVSQKKADAIKSALEPDNVDFPEGLSFDMENLDN
                     ALVFNFHSTGNMKKLTATIDEVLAHVQMALKVME"
     gene            2462..3724
                     /gene="serS"
                     /locus_tag="VGA92_04285"
     CDS             2462..3724
                     /gene="serS"
                     /locus_tag="VGA92_04285"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9845663.1"
                     /translation="MLDPKILKEKPELIRKMLKDRQVTFDLDSLINLDAKRRELIIKT
                     DELRKKKNTVSLDIASKKKAGQDAFSAISQMQLVSSELAALESEQLKVESDYARLAFS
                     IPNLVHESVPIGVDETANQEVRKWGKVPSFDYTIKDHIDISQSLDLVDLERAAKAAGA
                     RFYFLKNALVKLNQSLVHFALDFLDEKKYTLIQPPYMINKTSMEGAIIAQDFQDVIYK
                     VDGEDLFLIGTSEHAIAAMHSDEILEGKDLPIRYAGTSPCFRKEAGAHGRDQKGIFRV
                     HQFEKVEQFVFARPEESWKEHERMLSVVEEFYQKLEIPYRVMLLSSGDLGKISAKTYD
                     LEAWMAGQNAYREIVSCSNCLDFQARRLKIRFRDKTNEQTQYVHSLNSTLVATTRTLV
                     AIMENFQTKDGHISVPKPLQKYLGSSTI"
     gene            3800..4414
                     /locus_tag="VGA92_04290"
     CDS             3800..4414
                     /locus_tag="VGA92_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HEX9845664.1"
                     /translation="MARRKGGKVKDKWREKKWVTVIAPESFNNAPIAYVPITDEKKAI
                     GRVIEVTLFDILKGDPSQYQYKIYFQINRIDGEKAYSIFKRYEYAKEFLRSLIRRGSS
                     KVTYVMDVKTKDNYLFRIKVIALTHKKLNTSRKHALRLIANDVMTKTIGEMSIDQFVQ
                     ATCYGKINSDIMAAAKKVIRMRHVGLEKVKLIRTAEAQVTMLEA"
     gene            4425..4856
                     /locus_tag="VGA92_04295"
     CDS             4425..4856
                     /locus_tag="VGA92_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX9845665.1"
                     /translation="MEKRLEVDIQVIVHATEDIQKIFGAFKKVFDVDKEEFSENIVEG
                     HFENPITILSCKLKKKSSQGFVKALISKIPKDELELIIEDLQNRCDGSALHLRISKQS
                     LVRGLVRLGDTDPVKLRIYTPVYIEKDLIKTYSEILSQEFG"
     gene            5642..7099
                     /locus_tag="VGA92_04300"
     CDS             5642..7099
                     /locus_tag="VGA92_04300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9845666.1"
                     /translation="MNTDYDLIVAGGGLAGMVAAQSAAHHSNQRLSILVIDRNPPILL
                     GRKTVSGWICGDAVSKEAVDYMQKINVTWGKPELEHKVKGVMALSPDRETSIPFDGDG
                     YLLNRQQLPQSQYHEAQKRGINVDFEVNLNGLLYDGNQVVGVEGTNRKTNQPYKKTAK
                     VVIDATGVASMLRNGIKITTKIEKKIDRTDLESTGRHIMYFDKGEDDKSEFDPDYCLI
                     HLDQDIAPGGYGWVFPKGENKVNIGLGVEKTLLDKRNKRLGKHDDVTGLINQYVARNR
                     AIKNPRLSTDPADAHNATGNFQVSVRRQNDCMVANGFMLVGDSAWMPKPLDAGGIGPA
                     LIAGTIIGKNAVDAIESSDVTEKGLWQYNVDYVREYGYKTAGLEVFRRLLQMLTNDQI
                     DYGMKHFMGNMDVEAISRGEHPDFSMIGKMGMIIRGALNKKLADGLRYTTQQNKWLTE
                     HYANYPKSPEGFEEWHKKLLKTLNESFEHLAKWEN"
     gene            7132..>7411
                     /locus_tag="VGA92_04305"
     CDS             7132..>7411
                     /locus_tag="VGA92_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9845667.1"
                     /translation="MLQSSSTYLDWNNSLEILNTAYESGLFVLVIGPKGTGKTSLVRE
                     FATKKAANLESVNFSLRTRESHLVGTKTLSEGTVGFDEGILIKSMRSGS"
BASE COUNT         2438 a         1492 c         1744 g         1737 t
ORIGIN      
        1 cgcgattcct ccaaagactg ctgcggcgac tgcagaggcg cctaagacac cgtctgctgc
       61 tagatatgga gtacctgaac ctgttgctgg gttttctgct gcatagtcaa ctcgagcctt
      121 ttgaagagcc aatgtctcct ctaaggtgtt actaccagtg aatccttgtg gatcgacgtt
      181 gcccatgtat tgggcaaacg cttggtttgt catcattcca aatgacaaaa cagcgaaagc
      241 aacgattgtt gctccaaata ttgtcttatt catagtgttt acctgttgtc tgaagggcta
      301 ccgattctat ttaacttttt tctaattacc actgatgaca atttttcatg atttttggta
      361 acgtttatgt ttgaaccaaa attatttccg ccatgggacg agtacatacc cacagacacg
      421 gccagtcgca ctcaactagg ccgatcacac aaaaaagccc ttcttggctg acaactagtc
      481 caaaggagat agaggaactt gttgtaaaat acagcaagga cggcgtccca atgagccaaa
      541 tcggaatcag gctgcgagac cagcacgcaa ttccactggt aagacccatt gtaaaaaagt
      601 cgataaaaaa aattcttgag gacaatggaa ttactccaga gatcccagag gacctaaaca
      661 acattgtaaa aaaggcagtc aacttgcaaa aacacctcaa gacccacaga tccgataacc
      721 gaaacgtcag atcgcttgag ctaatagagg caaaggttca cagaatctcc acatactaca
      781 aaagaattgg agccctgcca caaaactgga aatataagtc cgtagttgct caacttgagt
      841 aatgagactt gaagaagcat tatcaaattt tgatcatacc atttctgatt gcataaaatc
      901 cggaaaagag atctgcgttg taacccacat tgactgcgac ggccttacct ctgggagcat
      961 aattaccaag gcactcatca ggcgcggcgc aaaggtcacg gcaaggactg ccactgagat
     1021 gaacggcgct gtcataaaaa acatggcagc cgactcgcgt gaatttcatg taattactga
     1081 tcttggcggc ggttttgcaa aacagttaga cgaggcgttg ggtcagaact ggtttgtttt
     1141 ggaccaccac gaagtaccaa aagaagaaca cgacaacgac cgggtcataa acgcctggaa
     1201 gtttgggata aacggcggaa cagaaatctg cgcaggcgga atggcatacc ttgctgcaaa
     1261 atcactggac aagcaaaaca tcgacttggc gccaatagct gtcgtatcgg cactaggcga
     1321 cagacaggac cagggagaaa gaaaatcatt tactggcaaa aacgccgaga ttgccgagga
     1381 ggcaaaaaaa tcaggccatc ttaaaatcga cttggatatt cttttggttg gacgcgagac
     1441 ccgacccctt gcagatgcac ttgcattcac atcgcagcca ttcattgagg gcctgacttg
     1501 gaacagggat gcgtgttttt cgctgatctc gtctgcgaaa attgccgcaa aggagggggg
     1561 aaggctgcga actccgtcgg atcttaacga agacgagaag aggcttttga ttgaggcaat
     1621 tacaaagttt gcaagcggcc agaatgcaaa ccaaatcatg gaggaattaa ttggatacac
     1681 ctacactttt ccaagcgagg acagacggaa ctttttgcgt gatgcacgcg agttttcaac
     1741 aatgctaaac tcttgcggaa gaattggcaa gtctggtgtt ggaattgcca tatgtatggg
     1801 cgacagaaac aagatgctca aggaaggaga aaccattcta gttgaataca gaaagatgat
     1861 ccgagagtac atgaatgttt taacaaatga gcggtggaga gttaatgact cgatgaacta
     1921 tcttatggta aacggcgagg gagtgattcc tgagaccatg actggaacca tttcgtcatt
     1981 gttagccggc tcgccaaaaa acgcaggcaa gataataatt ttgaggacaa acggcgagga
     2041 gaacacggtc aagttttcgt cgcgaaaatc gattggctgc aagtctggtg tgaatcttag
     2101 cgttatgatg aggactggtg ctgaaaagtt caatggaata ggtggcggac atgacgctgc
     2161 cgcgggggca cgaataacaa aggacaagct tgatggattt ctagacttcc tagaagatac
     2221 ggtaaatgga aaccaagatt caaatcagaa ttaatgacgt ttcgcaaaaa aaggccgatg
     2281 cgataaaaag cgcactagag cccgacaatg tggattttcc agagggcctc tcctttgata
     2341 tggaaaatct tgataacgca cttgttttta acttccacag tactggtaat atgaaaaaac
     2401 ttactgcaac aattgatgag gtattggcgc acgtgcagat ggcactaaaa gtgatggagt
     2461 aatgctagac ccaaagattc tcaaagaaaa gcctgagctc atacgaaaga tgctcaaaga
     2521 caggcaagtt acatttgact tggactcgct gattaaccta gatgcaaagc gacgcgagct
     2581 aatcataaag acagacgagc ttagaaaaaa gaaaaacacg gtttctttgg atattgcaag
     2641 caaaaaaaag gcgggccagg atgcattctc tgcaatttcg caaatgcaac tagtatcatc
     2701 ggagcttgca gcactagagt cagagcagct caaagtagag tcagactatg caaggcttgc
     2761 gttttcaatt cccaatctgg ttcacgagtc cgtcccaatt ggagttgatg agactgccaa
     2821 tcaggaggta agaaaatggg gcaaggtgcc tagctttgac tataccatca aagaccacat
     2881 cgatatttcg caaagccttg atctggtgga tctagagagg gctgcaaaag cggcaggtgc
     2941 gaggttttac tttttaaaaa atgccctagt caagctgaat caatccttgg tacattttgc
     3001 acttgatttt cttgatgaga aaaaatacac actcatccag ccgccataca tgatcaacaa
     3061 gacctccatg gagggcgcaa taattgcgca agactttcag gatgtaattt acaaagtgga
     3121 tggagaggat ctttttctaa tcggcacatc agagcacgca attgccgcaa tgcactctga
     3181 tgaaattctg gaaggaaagg atttgccaat ccgatatgcg gggacaagtc cgtgttttag
     3241 aaaggaggct ggggctcacg ggcgagacca gaaggggatt ttccgagtcc accagtttga
     3301 aaaagtagaa cagtttgttt ttgcaaggcc cgaagaatca tggaaggagc atgagcggat
     3361 gctttcagta gtcgaggagt tttatcaaaa actagagatc ccataccgag tgatgctttt
     3421 gtcaagtggc gaccttggaa aaatttctgc aaagacatac gacttggaag catggatggc
     3481 gggccaaaac gcgtaccgcg aaattgtatc atgttctaac tgtcttgact ttcaggcaag
     3541 gcgcctcaaa attcgattcc gcgataagac aaacgagcag acacaatatg tccactcgct
     3601 aaacagcaca cttgttgcaa caactagaac gcttgttgca atcatggaaa actttcagac
     3661 aaaggacggc cacatcagcg tcccaaaacc tttgcaaaaa tatctcggct caagtacaat
     3721 ttagttccgt agttattcct tagcatggta aggaaatggt aaggattaac ttatattttg
     3781 gatttaaagg tcgcagttat tggctcgaag aaagggtgga aaagtaaagg acaagtggcg
     3841 tgagaaaaaa tgggtcacag taattgcccc agagtcattt aacaacgccc cgatagctta
     3901 cgtgccaatc accgatgaga aaaaggcaat tgggcgcgtg atcgaagtaa cactctttga
     3961 tattctaaag ggagacccat cccagtacca gtacaagatt tactttcaga taaacagaat
     4021 tgatggcgaa aaggcgtatt ccattttcaa gcgatacgag tatgcaaaag agtttctgcg
     4081 ttctttgatc cgacgcggct caagcaaggt cacctatgtg atggacgtaa agacaaagga
     4141 caactatctt ttcagaatca aggtgattgc actaacccac aaaaagctaa acacatcacg
     4201 aaagcacgca ctcagactga ttgccaatga cgtgatgaca aagacaatag gcgagatgtc
     4261 aattgaccag tttgtccagg caacctgtta tggcaaaatt aattcagaca tcatggctgc
     4321 tgcaaagaag gtcatccgaa tgagacacgt cggcctcgaa aaggtaaagc tcatcagaac
     4381 cgcagaagcc caagtcacga tgctagaagc ctaagttttc actcatggaa aaaagacttg
     4441 aggtagacat ccaagtcata gtgcacgcaa ccgaagacat acaaaaaatt tttggtgcgt
     4501 tcaaaaaagt ctttgatgtc gacaaagaag agttttcgga aaacatagtt gaggggcatt
     4561 ttgagaatcc aatcaccata ctatcatgca agctaaagaa aaaaagttct cagggatttg
     4621 taaaagcatt gatttcaaaa attccaaaag acgaattgga gttaataatt gaggatctgc
     4681 aaaatcggtg tgacgggtct gccctgcatc ttagaatctc aaaacaatct ctagtcagag
     4741 ggctagtaag acttggtgat accgatccag tcaagctaag aatttacacg ccagtctaca
     4801 tagaaaagga tctcatcaag acatattctg agattctaag ccaagagttt ggttagtttt
     4861 ctttttgcca agttgtagtt ataacataat aaccagatcc ttaattagaa acaatggagt
     4921 aaagtattag ttgtcaattt caaaaacaaa gaaaaggacg aaatctgcgt ctaccaagga
     4981 tgagaatctt tggccagcgg aagaaaaggc ggtaaaacaa tacaaacggg gcaaacttgc
     5041 tgggaaacac tttgacaatg ttaaagatct tatcaaagat cttaacagct aaaaatgtgg
     5101 acgtttgaga gaaaaaatca attcaaacga caatacaaac atcttcattc cgaaattcag
     5161 aaaagagtaa atgatacatt aggtatttta gctgttgaac aaaatcctgc caaaccagaa
     5221 tttcacaaga tgggaaacat caaatgcctt catgcttatc ctgtaggacg acaataccgg
     5281 atattatacg acgtgaattt ccaaaagaga gaaatcgtgt taattcgggt tggtactcat
     5341 gctgatgtct acgactaatt tttcgtgact gaaccagcta aaaaggtcca aaaaacatca
     5401 tcgtcagatt ttgagattcc aagcattcat gtcgacgaaa ttttgacatg aatgacagca
     5461 atcaaaaact tggcaccgta tgattaaaca taatttaata tcattaagag tagtaaagag
     5521 ttgggaatga agagctacaa gccgctccag tctgagcaac gcagctgtga cgacgccgcg
     5581 acgatctgac ccaaaaaatt tcggattgtt ttaaataatg atcatttagc tgattgaata
     5641 gttgaataca gattatgatt tgattgttgc cggcggaggg ctagccggaa tggtagctgc
     5701 ccagtctgct gcgcatcact ctaaccaaag attatcaatt cttgtaatag acagaaaccc
     5761 gccaattctg cttggacgaa agacagtcag cggctggatt tgcggcgacg cagtatcaaa
     5821 ggaggcagta gattacatgc aaaaaatcaa tgtaacgtgg ggcaagccag agctggaaca
     5881 caaagtaaag ggcgtcatgg cgctatcgcc ggacagggag acttcgattc catttgatgg
     5941 cgacggttat ttgctaaaca ggcagcagct tccgcaaagc cagtatcacg aggcgcaaaa
     6001 acgcggaata aatgtggatt ttgaagttaa cttgaatggt ctcctatatg acggaaacca
     6061 ggtagtcgga gtcgagggaa caaacagaaa gacaaaccaa ccctacaaaa aaactgccaa
     6121 agttgtaatt gatgcgaccg gcgttgcatc gatgctccga aacggaatca aaataacaac
     6181 aaagattgaa aaaaaaatag acagaactga tctggagtca actggaaggc acatcatgta
     6241 ttttgacaaa ggcgaggatg ataaatcgga gtttgatccg gattattgcc tgattcattt
     6301 ggatcaggac atcgcacctg gcggatatgg gtgggtcttt ccaaaaggag aaaacaaggt
     6361 aaacatcggc cttggtgtcg aaaagacatt actggataaa agaaacaaga gattgggaaa
     6421 acacgatgac gtaaccggcc ttatcaacca gtacgttgca aggaatcgcg caatcaaaaa
     6481 cccaaggctt tcaactgatc cagcagatgc gcacaatgct actgggaact ttcaggtatc
     6541 agtccgtcga caaaacgact gcatggttgc aaacggcttc atgctagttg gtgattcggc
     6601 ctggatgcca aagccacttg atgccggagg aattggtcct gcactgattg ctgggaccat
     6661 cattggcaaa aacgcagtcg atgcaataga gtcaagcgat gttacagaaa agggactctg
     6721 gcagtacaat gtagattacg tcagagaata tggatacaag actgcagggc ttgaggtgtt
     6781 tagaagactg ctccagatgc tcacaaatga tcagattgat tacggaatga agcactttat
     6841 gggaaacatg gatgttgagg caataagtcg tggcgagcat ccagattttt ctatgattgg
     6901 gaaaatggga atgataattc gcggtgctct gaacaaaaag cttgcagatg ggctgcggta
     6961 cacaacgcag caaaacaagt ggctcacaga gcattatgca aactatccaa agagcccaga
     7021 gggatttgag gaatggcaca aaaagctgct aaagacactc aatgagtcct ttgagcacct
     7081 cgcaaaatgg gaaaactaat catttttaaa aagtaaagta cttttcaaat catgctgcaa
     7141 tcttcttcta catatctaga ctggaacaat tctcttgaga tcctaaacac ggcgtacgag
     7201 tcaggccttt ttgttttggt aattgggcca aagggaacag gcaagacatc gctggtacgc
     7261 gaatttgcaa caaaaaaggc agcaaaccta gaatctgtaa atttcagtct gcgaacgcgc
     7321 gagtcacatc tggtcggcac aaagacactc agtgagggaa cagtgggatt tgatgagggg
     7381 attttgataa aatcaatgag gagtggaagc a
//
LOCUS       DASWKH010000054         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2724071, whole genome shotgun sequence.
ACCESSION   DASWKH010000054 DASWKH010000000
VERSION     DASWKH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724071"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGA92_04310"
     CDS             <1..556
                     /locus_tag="VGA92_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX9845668.1"
                     /translation="HLGYINALLAGNALAVHDIEYSTLGTSLGMNIHDGTLAIRGHRN
                     HMQAINSVFKAGSIENMVKSRKLTKGIMYECVKNKVPFVLAGSLRDDGPLPDVITDVS
                     IAQQKYKEVLKGASMVIMVSTMLHSIATGNMLPADVKVIVVDINQPTVTKLMDRGTWQ
                     ALGIVSDVGAFLPMVAHEIKKLAK"
     gene            complement(563..1969)
                     /gene="gatB"
                     /locus_tag="VGA92_04315"
     CDS             complement(563..1969)
                     /gene="gatB"
                     /locus_tag="VGA92_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9845669.1"
                     /translation="MTKIGLDIHCQLTKLESKLFCSCKADYRGFEPNTNICPVCVGLP
                     GSLPRLNKKAIEKATVIAMALNCKTPQKIAFFRKNYFYPDLPKNFQITQLNIYGPTSI
                     GQEGKISVEGKEIRIRRIQLEEDPGRLIYEGSSERTQITLVDYNRAGTPLVEIVTEPD
                     FENPKQVRLFLNILSDLLENLGVSDPSLEGAMRADANVSIEGGSKVEIKNINSFYDLE
                     KALHFELTRQQSLAERKIEIIQETRHWDDKRKITISARSKEGEEDYRYFLEGDIPWVV
                     IEPQIISRLKKDMPESISSKRERYIKDFGIPSQVADVLSSDKFYSDLFEQAHDQKNAK
                     EIANFITTDLMGFVDTKEKRKESKLTAKHLADLANAIIENKITRNSSKNVLQTIVKSG
                     KSVSQLISELDLGHVSDEAELSKVIDEVIIEESKAVEEAKQKPETINYLVGKVMKKTK
                     GKADATLTLNLLKKKLSK"
     gene            complement(1966..>2166)
                     /locus_tag="VGA92_04320"
     CDS             complement(1966..>2166)
                     /locus_tag="VGA92_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9845670.1"
                     /translation="PIAMFLIDFNTTTANLTGKPAISVPYEISNGLPIGIQIIADSMQ
                     EKSLFQAAYALESTTNLPKVPL"
BASE COUNT          596 a          395 c          405 g          770 t
ORIGIN      
        1 ccacttaggt tacataaatg ccctgcttgc aggaaatgct cttgctgtac atgatataga
       61 atattctaca cttgggacat cgcttggaat gaatatacat gatggtacat tagctataag
      121 aggacaccga aaccacatgc aggcaataaa ctctgttttc aaagcaggat ctattgaaaa
      181 catggtaaag agcaggaaac tgacaaaagg gatcatgtac gagtgtgtaa aaaataaggt
      241 gccttttgtt ttagctggct ctttgcgtga tgatggtcca ttacctgatg tcataacaga
      301 tgtttccatt gcacagcaaa aatacaaaga ggttctaaaa ggtgcaagca tggtgatcat
      361 ggtgtctacg atgcttcact caattgccac tggaaacatg ctacctgcag atgttaaggt
      421 aatagttgtt gacatcaatc aacctacagt tacaaaactc atggatagag gcacttggca
      481 ggcactaggc atagtctcag atgtaggtgc atttcttcct atggtcgcac atgaaataaa
      541 aaaactagca aaataactac aattactttg atagtttctt ttttaataaa ttaagagtaa
      601 gagtagcatc tgcctttcct tttgtttttt tcattacttt tccaaccaaa tagttgattg
      661 tttctggttt ttgttttgct tcctctacgg ctttactttc ttctattatt acctcatcta
      721 ttactttaga taattctgcc tcatctgaaa catggcccaa gtcaagctca gaaattaact
      781 gtgatactga tttgccactt tttactatgg tttggagcac attttttgaa gaatttcgtg
      841 taattttgtt ttcaataata gcatttgcca aatctgcaag atgttttgcg gtaagtttag
      901 attcttttct tttttctttt gtatcaacaa atcccattaa atcagttgta ataaaattgg
      961 caatctcttt tgcgtttttt tgatcatgtg cttgttcaaa caaatccgaa tagaatttgt
     1021 ctgatgataa aacatctgca acttgtgaag ggatgccaaa gtctttgatg tagcgttcac
     1081 gttttgagct tatgctttct ggcatgtcct tttttaatct tgaaatgatt tgtggttcaa
     1141 ttacaaccca tggtatatct ccttcaagaa aatatctata gtcttcttct ccttctttac
     1201 ttcttgcaga tattgtgatt tttcttttat catcccagtg tcgtgtttct tgaattatct
     1261 caattttgcg ttcagctaga ctttgttgtc tagtaagttc aaagtgtaat gccttttcta
     1321 aatcataaaa tgagttgata tttttgattt caacttttga gccgccttct attgaaacgt
     1381 ttgcgtctgc tctcattgca ccttcaagtg atgggtctga tactcccaag ttttcgagta
     1441 aatccgataa tatgtttaaa aatagtcgaa cttgttttgg attttcaaaa tcaggctctg
     1501 ttacaatttc aacaagggga gttccagcac ggttataatc aacaagtgtg atttgtgttc
     1561 gctccgagct tccctcataa attaacctgc cagggtcttc ttcaagctgg attcttcgta
     1621 tccggatttc ttttccctct acgctgattt ttccttcttg gccaatactt gttggaccat
     1681 agatgtttag ctgcgtaatc tgaaagtttt ttggtaaatc tgggtaaaag tagtttttcc
     1741 taaaaaatgc aattttttgt ggagttttgc agtttagcgc cattgcaata actgtggcct
     1801 tttcaatggc ctttttgttt agccttggaa gtgaacctgg gaggccaaca cagacaggac
     1861 agatgttcgt atttggttca aatccacgat aatctgcctt gcaagaacaa aacagcttgc
     1921 tctcaagctt tgttagctgg cagtgaatgt caagaccaat ctttgtcata gtggcacctt
     1981 tggaagattt gttgtactct caagagcata tgctgcctga aaaagcgatt tttcttgcat
     2041 cgagtctgca ataatttgaa ttccaattgg aagtccattt gaaatctcat atgggacaga
     2101 aattgcaggc ttgccagtaa gatttgcagt tgttgtgtta aaatcaatta gaaacattgc
     2161 tatagg
//
LOCUS       DASWKH010000055         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2729792, whole genome shotgun sequence.
ACCESSION   DASWKH010000055 DASWKH010000000
VERSION     DASWKH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2729792"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..449)
                     /gene="pfdA"
                     /locus_tag="VGA92_04325"
     CDS             complement(30..449)
                     /gene="pfdA"
                     /locus_tag="VGA92_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HEX9845671.1"
                     /translation="MSEEQAQQLLYQMQILESFAANLDQKEAAIINILREAISSIDTI
                     KGTQNQEAESLVPVGLGTYVKAVLQGNSKIVVDVGAGMAVEKDHDSAINYLESRIKEL
                     QVALNETNAQKHETMLRLEQLKEQMNHLIQPSNPPQQ"
     gene            508..927
                     /locus_tag="VGA92_04330"
     CDS             508..927
                     /locus_tag="VGA92_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9845672.1"
                     /translation="MILKKGGKFVKPKSKWIRPYKKEHPTDKLKVKLILNERIKGDRI
                     VGLKARIETGQVHQLHTHENEYVLVYSLKGKCLVTVGKNTKVVLPDTLIFIPPKVPHR
                     FYNKFSSAWEGVAFAIGTKSKIKNIWMQDNKKEGKII"
     gene            complement(920..1540)
                     /locus_tag="VGA92_04335"
     CDS             complement(920..1540)
                     /locus_tag="VGA92_04335"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX9845673.1"
                     /translation="MGKYTLPEVPYAYDALEPHIDAKTMEIHHSKHHQAYTNGLNDAW
                     DKLPADLQSKDLLDVLANISQVPEAVRGAINFHGGGYDNHRLFWNNMKPNGGGQPGGA
                     ISDAVNKTFGDFNGFKEQFSAKTTAIQGSGWGWLVFNPKSKNVEYKSMPNQTSPRTEG
                     LVPLLGLDVWEHAYYLKYQNKRPDYVAAWWNVVNWDEVDNRYSKVK"
     gene            1669..>3677
                     /locus_tag="VGA92_04340"
     CDS             1669..>3677
                     /locus_tag="VGA92_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9845674.1"
                     /translation="MKMRLVFCVLVIVIGFSLLSQKIQHDPPLEILLDKSSSYVGVER
                     PHELGFNGDNIKIAVIDTGVDYNHPDLFGFGPDGKVIGGYDFIENDDSPLDTNGHGTE
                     VAGIIAANGNLRGVAPNAKILAYRVSDTGNSVSSDLIIKAVERAVQDGADIINISLGV
                     NRTNDKIEDAINDAVKKGIIVITAAGNSGPDLRTIGSPGKDPNAITVGATYNNITASL
                     VATLEIDSIRIQILPMVGLEPLNGPITSEIVFGKYGRQEDLKDVDAKDKILLIERGSD
                     VEGELVYFSQKEKNSANAGAKAIIVYNNEPGIFLGDLKNKLEGPDYKPRIPAVSMSGE
                     DGIIVKSMLENKTIGTINTFYHPDFVSFFSSRGPVSPFYIKPNLVAPGVFVNTTSIAG
                     GYNLTSGTSFAAPHVSGAVAILLQKNPTLTPQQIGSIISTTADPISDTFGTLFTQEIS
                     GVGRLNVTKAFDANLIILPSFAIFDLSPHTKTQTVAFDLKTINDEKPNITVDFKIDQN
                     AAKLDYTIDENTLTVKATLTDQKVGQYEGSIIVADQKTTYHIPVLLRISNGSINVIEN
                     NGRLDFEIDSSDEWSYAKISVFNTDYRIVDSTSATPASTGSILIHDTGTYWIQAELKS
                     GKDTINLYNTAIVKNSAEPSFDISDIGLPAQPLLIIIVIASVIVMV"
BASE COUNT         1102 a          720 c          762 g         1093 t
ORIGIN      
        1 gtacgcagtt tgtcaaacat taagaccact tattgttgtg gggggtttga gggttgaatc
       61 aggtgattca tttgttcttt tagttgttct agtcttaaca ttgtttcatg tttttgtgcg
      121 tttgtttcat ttagcgctac ctgtagttcc ttgattcgcg attcaagata atttattgca
      181 gagtcatggt ctttttctac tgccattcct gctccaacat caacaactat ctttgagttt
      241 ccctggagta ctgcttttac gtatgtccca agcccaactg ggacgagtga ttccgcttcc
      301 tggttctgcg ttcctttgat tgtgtctatt gatgatattg cttcacgcag gatgtttatg
      361 atggcagctt ctttttgatc tagatttgct gcaaatgact caagaatttg catttggtaa
      421 agaagttgct gggcctgttc ttcactcatt tacaaaaaaa gtattcagat ggctataaat
      481 gcattcatga tgaactggag tcagaaattg attttaaaga aaggcgggaa gttcgtaaaa
      541 ccaaaatcaa aatggattcg tccttacaaa aaggaacacc cgacagataa gctcaaagtt
      601 aaactgatat taaatgaaag aatcaaagga gaccgaattg ttggtttgaa ggcaagaatt
      661 gaaacaggcc aagtgcatca gcttcacacg catgaaaacg aatacgtcct cgtttattca
      721 cttaaaggaa agtgtctagt tacagttggg aaaaatacaa aggtcgtttt gcccgacacg
      781 ttgatcttta tcccgcccaa agttccacat cgattttaca ataaattttc ttcagcatgg
      841 gagggagtgg cgtttgcaat aggcaccaaa tctaagatca aaaatatctg gatgcaggac
      901 aacaaaaaag agggtaagat tatttaactt tggaataacg attgtctact tcgtcccagt
      961 ttacaacgtt ccaccatgca gctacatagt caggtctttt gttttgatat ttgagatagt
     1021 atgcgtgttc ccaaacatct agacctagga gtggaacaag tccttctgtt cttggactgg
     1081 tctgattagg cattgatttg tattcaacat tttttgattt tggattgaag actaaccaac
     1141 cccaaccgct tccttgtatt gcagtagttt ttgctgaaaa ctgttctttg aaaccattga
     1201 agtctccaaa tgttttgttg actgcgtctg atattgcgcc gccgggttga cctccgccgt
     1261 ttggtttcat gttattccag aatagcctat ggttgtcata accaccacca tggaagttga
     1321 ttgcgcctcg taccgcttct ggcacttggc ttatgtttgc taagacatca agcagatctt
     1381 ttgattgcag gtctgctgga agcttgtccc aggcatcatt caggccgttt gtgtacgcct
     1441 gatggtgctt tgagtggtgt atttccattg tttttgcgtc aatgtgtggt tctaacgcgt
     1501 cataagcata tggtacttca ggaagtgtgt attttcccat gtaaactgaa agttttttca
     1561 taatttaatg atttaccttt gttttgtttt ttatcaagag tttcaaataa tttgcaaatt
     1621 aaccaaaaga cgtaagataa attgtgtttg tttataccta aagataaaat gaagatgcgt
     1681 ctggttttct gcgtacttgt gattgtgatc ggtttttcac tgctttcaca aaaaattcag
     1741 cacgatccac ctttggagat tcttcttgac aaaagtagta gctatgttgg tgttgaacgt
     1801 ccacatgagt taggtttcaa tggggacaac atcaagattg ccgtaatcga tactggtgtt
     1861 gattataatc atcccgatct ttttggtttt ggaccagatg gcaaggtaat cgggggctat
     1921 gattttattg aaaacgacga ttcaccatta gatacaaacg gtcatggaac agaggtagct
     1981 ggcataatag ctgcaaacgg taacctaaga ggagttgccc ctaacgcaaa aattttggcg
     2041 tacagggtct cagatacagg aaattcagtg tcttccgatt taatcatcaa ggcagttgaa
     2101 cgggcagtgc aagatggcgc agacataatc aacatcagtc ttggcgtaaa tcgaactaat
     2161 gacaaaattg aagacgcaat aaacgacgct gtcaaaaaag gaatcatcgt cataactgca
     2221 gcaggaaaca gcggtcctga cctaaggaca atagggagtc ctggaaaaga cccaaatgca
     2281 atcacagttg gcgccacata caacaacatc acagcaagcc tagtagccac cctcgagatt
     2341 gacagtatac gaattcagat tctgcccatg gttggactag agccacttaa cggtccaata
     2401 acatctgaaa ttgtgtttgg caaatacggc cgtcaggaag atctaaagga tgttgatgca
     2461 aaagacaaaa ttttgctaat tgagagggga agtgatgttg aaggagagct tgtttatttt
     2521 tcacaaaagg aaaaaaactc tgcaaatgct ggagcaaaag caatcattgt ttacaacaac
     2581 gagccgggca tatttcttgg tgatctaaaa aacaagctgg aaggcccaga ctacaaaccg
     2641 agaattcctg ccgtttccat gtcaggcgaa gacggaatca tagtaaaatc aatgttagaa
     2701 aacaaaacaa ttggcacgat aaacacattc taccatccag actttgtttc atttttcagc
     2761 tcacgcggcc ccgtttctcc attttacata aaaccaaatc ttgttgcgcc tggagtcttt
     2821 gtaaatacaa cctcaattgc tggaggatat aatcttacaa gcggcacgag ttttgccgcc
     2881 cctcacgttt caggcgcagt tgccatactg ttacaaaaaa atcccacact caccccccaa
     2941 caaatcggtt ctatcatttc cactacggca gacccaatat cagatacttt tgggactctg
     3001 tttacacaag agattagtgg tgtgggaaga ctgaatgtga caaaagcatt tgatgcaaat
     3061 ctaatcattt tgccgtcttt tgccatcttt gatttatcac cacataccaa aacccagact
     3121 gtggcctttg atctaaaaac aatcaatgat gaaaaaccaa acataacagt tgatttcaag
     3181 attgaccaaa acgcagccaa attagattac acgattgacg agaatacgtt gacagtaaaa
     3241 gcaaccttga cagaccaaaa agttggccaa tacgaaggca gtatcatagt tgccgaccag
     3301 aaaaccacct accatattcc cgtcttgttg cgaatttcaa atggttctat taacgtaatt
     3361 gaaaataacg gaaggctgga ttttgagatt gattcatcag atgagtggtc gtatgcaaaa
     3421 atttccgtgt ttaatacaga ctataggatt gtcgatagca cgtccgcaac tcctgcaagc
     3481 acaggatcaa ttttgattca tgatacgggc acttattgga ttcaagctga gctaaaatca
     3541 ggaaaagaca ccattaattt gtacaatact gcgatagtca agaactcagc agagccaagt
     3601 tttgatatta gtgatattgg attgccagca caaccgcttt tgataattat tgtgattgca
     3661 agcgttattg taatggt
//
LOCUS       DASWKH010000056         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2731669, whole genome shotgun sequence.
ACCESSION   DASWKH010000056 DASWKH010000000
VERSION     DASWKH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731669"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1835
                     /gene="pckA"
                     /locus_tag="VGA92_04345"
     CDS             234..1835
                     /gene="pckA"
                     /locus_tag="VGA92_04345"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX9845675.1"
                     /translation="MVDSKTTLTAQFIAQIEEFGLKPSKIHRNLSVEKLVEAVVHGNE
                     GIVTSTGSLAVKTGKYTGRSPDDRYIVDDDETHDNVDWGKINHPFPIDRFEKIFSKMK
                     KFVEGKEIFVFDGFAGADRENRLAIRVINDHAWQSLFARQLFVKPSAAELEAHKPEFT
                     VMCINDFEAIPEIDGTGSNVFILINLTKKLVLIGGTSYAGEVKKSIFSVMNYLLPNKG
                     VFPMHCSANIGKSGDTALFFGLSGTGKTSLSADPNRMLIGDDEHGWAAKGVFNFEGGC
                     YAKCINLNQESEPQIWNAIKSGAVLENVVIDKKTLKPNFDDGSLTENTRAAYPLEFIP
                     TAVFPSVGGNPKVIVFLTADALGVLPPLSKLTKEGAMYHFMSGYTSKLAGTERGIKEP
                     RVIFSECFGAPFMPRPASVYAELLGEKIKQHNTVVYLINTGWSGGPYGVGKRINIKHS
                     RAMVTAALTGALDIVKYRHDDLFNLDVPTECPEVPSEVLDPKTTWQDRDAYELSARKL
                     AQMFVDNFKKFKNTAPEIINAGPKP"
BASE COUNT          614 a          374 c          407 g          470 t
ORIGIN      
        1 gatccttctg tatcgccaac tttcctgcct gacaaactct ggcctgttct gagatacttt
       61 ctgtcttaac gcaagccttt ccttattgat ggtcaacaaa cacaccactt ggattttaca
      121 ataaataggt ttacttgaga tctagagcaa aaatggcaaa aaataataat gaaccttgga
      181 ctgattgaat attcaatgaa cgtcctttgt tctttgaccc aaggtgaaac taaatggtag
      241 actctaaaac aactctaacg gcgcaattta tcgcacagat tgaggaattt gggctcaagc
      301 catcaaagat tcacagaaat ctgtctgttg aaaaactagt ggaggcggta gtacacggaa
      361 acgaaggaat agttacaagt accggctctc ttgccgtaaa aaccggcaaa tacacaggaa
      421 gatcaccaga cgaccgatac atcgtagatg atgatgagac tcacgacaat gtagattggg
      481 gaaagataaa ccacccattt ccaattgata gattcgagaa gattttttcc aagatgaaaa
      541 aattcgtaga aggaaaggag atctttgtat ttgatggctt tgctggggct gacagagaaa
      601 acaggttggc aatcagggtg ataaacgatc atgcttggca aagccttttt gcaagacagc
      661 tgtttgtaaa accatctgca gcagaacttg aagctcacaa gccggaattt actgtaatgt
      721 gcataaatga ctttgaggca attcctgaaa ttgatggcac tggatcaaac gttttcatac
      781 tgatcaactt aacaaagaaa ctcgttttga ttggaggaac gagctatgca ggagaggtga
      841 aaaaatcaat cttttctgta atgaattatc ttttgccaaa caaaggagta ttcccaatgc
      901 attgttctgc aaacatcggc aaaagcggtg acactgcgtt attttttgga ttgtcgggaa
      961 caggaaagac aagcttgtct gctgatccaa acagaatgct aattggtgat gatgagcacg
     1021 gttgggctgc caaaggcgtt ttcaactttg aaggcggatg ctatgcaaaa tgcataaacc
     1081 tcaatcaaga gtccgaacca caaatttgga acgcaatcaa gtcgggcgca gtactagaga
     1141 atgtggtaat tgacaagaaa acactcaaac caaattttga tgacggctcg ctaactgaaa
     1201 acacaagagc cgcatatcca ttagagttca ttccaacagc agtattccca agtgttgggg
     1261 gaaatcccaa agtaattgtg tttcttaccg ccgatgcact aggggttctt ccgccacttt
     1321 cgaaacttac caaggaggga gcgatgtatc actttatgtc cggttataca agcaagttag
     1381 ctggaacaga acgaggaatc aaagaaccaa gagtgatatt ttcagagtgt tttggtgcgc
     1441 cgtttatgcc aagaccggct tcagtttacg cagaactttt gggcgaaaaa atcaaacaac
     1501 acaatactgt ggtgtaccta attaacactg gatggtccgg aggcccatat ggtgtgggca
     1561 agagaataaa cataaagcat agccgcgcaa tggtgactgc ggcattgaca ggagcacttg
     1621 atatagtaaa atatcgacat gatgatctgt tcaacttgga tgtgccaaca gaatgtccag
     1681 aagttccatc cgaggtctta gatccaaaga ctacatggca ggacagggac gcatacgaac
     1741 tttctgcacg aaaactcgct caaatgtttg tagacaactt taaaaaattt aaaaacactg
     1801 caccagaaat cataaacgcc gggccaaaac cctgatcact ttcgaattac taatccaacc
     1861 attac
//
LOCUS       DASWKH010000057         6780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_522166, whole genome shotgun sequence.
ACCESSION   DASWKH010000057 DASWKH010000000
VERSION     DASWKH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522166"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..694
                     /locus_tag="VGA92_04350"
     CDS             194..694
                     /locus_tag="VGA92_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845676.1"
                     /translation="MNVIPLFSDRRTASAMWNETVHWWSDHDIKLRFVEAEDKYWFIL
                     ASETRRPDSNRSFFKLLDKSDNYTRFKKGSMGEAYLRFGTYSQKEEKDVKKNMVCNCG
                     HTKEDHDGACLVEECRCEKFISFVINLLKKKKTITNIKFLEEKDVKDDAVAWNCLYVN
                     KYKNSE"
     gene            complement(695..862)
                     /locus_tag="VGA92_04355"
     CDS             complement(695..862)
                     /locus_tag="VGA92_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845677.1"
                     /translation="MKDVDKCVLCNGPMDHKYLGMKDWNIQGHMCSKCYTKKISEHYP
                     GQHVRVNLDKE"
     gene            complement(1033..2784)
                     /locus_tag="VGA92_04360"
     CDS             complement(1033..2784)
                     /locus_tag="VGA92_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HEX9845678.1"
                     /translation="MAEKSSGLLEYIPGSHSLLLQKKIEQPLEGFAQNIKESLVEYTE
                     SGKSDVEKGHNFLHWVLTRVFEATEDDASDAIIDGANDLGIDAYLPVDFSDNKIRLFQ
                     SKYGTAHSEEAIAKFKEDVKRLLTKDVTKMRPELANLVTKIQEKNLKIECCYVTSQQV
                     NYDDESVEIIDIDKIVRSLWDRIKKPAAGKKSKIKLEKMIHYKNTVLGVLKLRELTDF
                     VTKNRDFVFESNIRQWMQFKTNVNKGIRETLQNTPDKFFYYNNGITIVVSSFEELQEN
                     IMMLHAPQIVNGAQTSNSILDHAKRTNNLDGSITVTIIKADDELDQNNITKYRNSQNS
                     VRGKDLVSLMDFHKSIKSQLENYGYFYEIQAGSFDSKTKSLQAEFKGDGIYNRYLPEN
                     HKKVIVAKDAIQVLIAGIEQRPTESYSSPAQFLPRGSKYDDVFNENLKDDYRLLLYPY
                     LVKEFAKKSLGYGKRGGHKTKRYATLFFVAVYFRIIYKSILGTRGDFKEDIVKMEPIF
                     KSNKLNERILKLADVVVTKFLEDTVVDDEMEIANTKHNFFSHHVWNEAMLRVVDKKIR
                     QEEDEIESIKKLVNSLL"
     gene            2913..3308
                     /locus_tag="VGA92_04365"
     CDS             2913..3308
                     /locus_tag="VGA92_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9845679.1"
                     /translation="MILILSVVVIILAGVIVTISLESNVTESDNFMADQRTVLITVSS
                     SRPGCEATNTCYVPAEITASSGEIITWVNEDSAFHTVTSGYYDTYDGIFDSGQLDPAE
                     KFSYSFDETGSFHYYCKLHPWMEGMVVVK"
     gene            complement(3308..3379)
                     /locus_tag="VGA92_04370"
     tRNA            complement(3308..3379)
                     /locus_tag="VGA92_04370"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3413..3634)
                     /locus_tag="VGA92_04375"
     CDS             complement(3413..3634)
                     /locus_tag="VGA92_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HEX9845680.1"
                     /translation="MKSSELGMSAMYRILKKAGAERVSDESADELRRVLEEVASTIAK
                     SAVEMSNHAGRKTIRGEDVKLASKALIKT"
     gene            3721..4479
                     /locus_tag="VGA92_04380"
     CDS             3721..4479
                     /locus_tag="VGA92_04380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9845681.1"
                     /translation="MTWLFDAHIHLSDSEYKDDIDLIINSMKKVGLKACAVSMDNASS
                     QTTLEIAKKSDLVLPFVGIHPEKASDDLESNISLIRKNSQSISGIGEIGLDRTYTSSD
                     EQFARQKNVFTKLLEEAENLKKPVSVHSRRTLDDIFEIIPSYRISGFLLHWFDGNKKQ
                     LKRAMDLGCFVSYGPLTVYSQDKQVLVSNTERDKILVETDGPVKFSRCFGMKAAQITF
                     IPSVVFCVSKILGQSYDECASVLEANSKNYLGIV"
     gene            4504..4827
                     /locus_tag="VGA92_04385"
     CDS             4504..4827
                     /locus_tag="VGA92_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845682.1"
                     /translation="MDRIKRLSMLVLENHRDKFTTNFAENKKTLDQLVIVRSKGLKNE
                     VAGYITKLLKREVDTKTTTDQEEQEDQEVEQVEAQEVVEAEEETAEDSTPQEILVENQ
                     SQESS"
     gene            4841..5614
                     /gene="cgi121"
                     /locus_tag="VGA92_04390"
     CDS             4841..5614
                     /gene="cgi121"
                     /locus_tag="VGA92_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HEX9845683.1"
                     /translation="MITIKFLGGAKKSFGTALISADLDNVAIRDLLAHLLSIKPKNTL
                     DLDTKNILVAVNGIDSSALDGQNTVLHSGDIISIIPIIHGGTQRLQFKIYNTWVELFS
                     VTYERGKNYDFLALTRKKFPDLIMEGVSSRCILSITHAKKIIGLSLFAKKHNILLSKK
                     LQTDILLRFAATTQISEAIKAVGIEGHDGFTIIAMGKTSSLNKLGSFLATHLVSTDYQ
                     KNFKPLQKQFKISKKHLDAVDSKTPLEDLMVEKAAVLFH"
     gene            complement(5603..6124)
                     /locus_tag="VGA92_04395"
     CDS             complement(5603..6124)
                     /locus_tag="VGA92_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX9845684.1"
                     /translation="MDLIVTCARHFEDETKEEIAGILDELGDPKAQVSITDMSGILTV
                     QTSVNSAEIIKKIHEKLEDEPWSIRYTLRIIPIFQTVNTNVESITEAALNQIQKIKPD
                     ETYRITIEKRNSGISSSEIISQIAEKVKNKVSLEKHDWIILVEVLGAISGVSVLKDAD
                     VLSVERVKRKSVE"
     gene            complement(6299..6586)
                     /locus_tag="VGA92_04400"
     CDS             complement(6299..6586)
                     /locus_tag="VGA92_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9845685.1"
                     /translation="MSAYRTHMQIIGDILTTARDNLPDEHGASITYLIRKANISHGRI
                     SKMLGTLVAQGLLEQTNSSGACKYKISNVGREFLQAYNTFNKFADSFGLTI"
BASE COUNT         2027 a         1346 c         1316 g         2091 t
ORIGIN      
        1 catcttttta tataccgata atttgacgtt ctaaaagaga ctgccatatt ggcgaattga
       61 tccaacaatc taagaggtga gtctccgatc atattaataa tcaaactatg tacttttcct
      121 taaagtgtca ggcatctcaa atccttggta tcaggacttt ttgggtgtgg cgtataggta
      181 ttttgatctt agaatgaatg tgatcccctt gttttcagac cgcagaactg cgtctgctat
      241 gtggaatgaa accgttcatt ggtggagtga tcacgacata aaactcagat ttgtggaagc
      301 cgaagacaaa tactggttca tcttagcatc cgaaacaagg aggccggata gcaacagatc
      361 gtttttcaag cttttggata aatcagacaa ctatacacga ttcaaaaagg gaagcatggg
      421 ggaggcgtat ctgaggtttg gtacctattc acaaaaagag gaaaaggacg taaagaagaa
      481 catggtatgc aactgcggcc acacaaaaga ggatcatgat ggggcatgcc ttgtggagga
      541 gtgcagatgt gaaaaattca taagctttgt gataaacctt ctaaaaaaga aaaaaaccat
      601 cactaatatc aaattccttg aggaaaaaga cgtcaaagat gacgcagtag cttggaactg
      661 cctttacgtt aacaagtaca aaaactctga atagctactc cttatccaaa ttaacacgta
      721 cgtgttggcc aggataatgc tcagaaatct tttttgtata acatttgcta cacatgtggc
      781 cttggatgtt ccagtctttc attccaagat atttgtgatc cattgggccg ttgcacaaaa
      841 cacatttgtc gacgtctttc aagcaatctc caaattcaga actctatata aaaatcatgt
      901 tcatgttttg atatggaaaa aaaatttgtt aaatattagt gcgtagcaga aaagctgcga
      961 tagagtgtct ggccaatgta aatctcctgc aatttttaca tggcctgatg cctatcgcaa
     1021 tttatgataa ttctaaagca aactgtttac tagtttcttg atgctctcaa tctcgtcttc
     1081 ttcttgtcgt atctttttgt caactactcg aagcatcgcc tcgttccata cgtggtgaga
     1141 aaagaaattg tgttttgtgt tggcaatttc catctcgtcg tcgactacgg tgtcttctaa
     1201 gaacttggtc accactacat cggccagctt caaaattcgc tcattgagct tgttgctttt
     1261 gaaaattggc tccatcttta caatgtcttc tttaaaatct ccccttgtgc caagaatgct
     1321 tttgtagatg attctaaaat acactgcaac aaagaacaag gttgcatatc gttttgtttt
     1381 gtggccgccg cgcttgccgt atcctaagga ttttttggca aattctttta caaggtatgg
     1441 ataaagcaat aaccgataat cgtcttttag gttttcatta aagacatcgt cgtatttgct
     1501 gcctcttggt aggaactgcg caggtgaact atacgattcc gttggtctct gttcaattcc
     1561 tgcaataagt acctgaattg catcctttgc aacaattact tttttgtgat tctcaggaag
     1621 ataccggttg tagattccat ctcctttgaa ctctgcttgc agtgatttgg tcttgctatc
     1681 aaaggagcct gcctgaatct cataaaaata tccgtaattt tcaagctggg atttaatgga
     1741 tttgtgaaaa tccatcagtg atacaagatc ctttcccctc acagagtttt gcgagttcct
     1801 atactttgta atgttgtttt ggtccagctc gtcatctgct ttgataattg ttactgtaat
     1861 acttccatca agattgttgg ttctttttgc atgatccaaa atagagtttg atgtctgagc
     1921 cccattgact atttgtggcg catgaagcat catgatgttt tcctgaagct cctcaaaact
     1981 gctaacgaca attgttattc cgttattata ataaaaaaac ttgtcagggg tgttttgaag
     2041 tgtttctctg attcccttgt ttacgtttgt cttaaactgc atccactgtc ttatgttgga
     2101 ttcgaataca aaatcgcggt tttttgtcac aaaatcggtc agctctcgaa gctttaacac
     2161 gccaagaacg gtgttcttgt agtggatcat cttttccaat tttatctttg atttcttacc
     2221 ggctgctggc tttttgattc gatcccagag gcttcgtact attttgtcaa tatcgatgat
     2281 ttcgactgat tcgtcgtcat agtttacttg ctggcttgtt acataacaac actctatctt
     2341 taggttcttt tcctgaattt ttgtaactag atttgcaagc tccggccgca tctttgttac
     2401 gtccttggta agtagccttt tgacatcttc tttgaacttg gcaattgctt cttcagaatg
     2461 cgcagtccca tactttgact gaaaaagcct tatcttatta tcggaaaaat ccacaggcaa
     2521 gtaggcatct atgccgagat cgtttgcacc atcaattatt gcgtctgatg catcgtcttc
     2581 tgttgcctca aagaccctag tcaaaaccca gtgtaaaaaa ttatggccct tttcgacatc
     2641 gcttttgcca ctctccgtat actcgactaa actttctttg atgttttggg caaacccctc
     2701 aagaggctgc tcgatttttt tctgcaaaag aagcgaatga gatccaggga tgtattctag
     2761 cagtcctgaa cttttttctg ccacaacctg tgccttgtaa tagtcggtat tagaaaagct
     2821 ggttattata taataaccac atctgctggc aagacgcaaa aaactctaca aaataaattc
     2881 tcagcacata ttgctgatcg tagttgtcaa aaatgatcct aattttgtcc gtagtggtaa
     2941 tcattcttgc tggggtgata gtgacgatca gcctagaaag caacgttact gaaagcgaca
     3001 acttcatggc tgatcagcgc acagtactga tcaccgtctc ctcatcgagg cccggctgtg
     3061 aggctaccaa tacctgttat gtgccagctg aaattacggc tagttctggg gagataataa
     3121 catgggtaaa tgaagactcg gcatttcata ctgttactag cgggtactat gatacgtatg
     3181 atggtatctt tgatagcggc caacttgatc cggcagaaaa attctcatac agttttgatg
     3241 agacaggcag ctttcattat tattgcaagt tgcatccttg gatggaagga atggtggtag
     3301 taaaataagg tgaggaaggg attcgaaccc ttatagattc gctctgcagg cgaacgcata
     3361 gccgctctgc cacctcaccg caaaaaaaat tcgattcaac aagaattaaa ctctatgttt
     3421 tgataagggc ctttgatgcc agcttgacat cctcgccacg aattgttttt cttcccgcgt
     3481 gatttgacat ctcaactgca ctctttgcta ttgtggaggc aacctcttca agaacccttc
     3541 tgagctcgtc tgccgactcg tcactgaccc gttcagcgcc ggcctttttt agaattctat
     3601 acattgcaga catgcccaac tctgatgatt tcattacaat gaatcaacaa aatggcccga
     3661 taaaagaatg tggttaaaaa attctacaga cttatttagc tataaaaatt cacagaaatc
     3721 atgacttggc tttttgacgc gcacatacat ctctccgact cggaatacaa agacgacatt
     3781 gacctaatta ttaactcaat gaaaaaggtc ggccttaagg cctgtgcagt ttcaatggat
     3841 aatgcctcgt cacaaaccac acttgagatt gcaaaaaaaa gtgaccttgt tttgccattt
     3901 gtaggaattc atccagaaaa ggcatctgat gatctggaat caaacatatc tcttatccgc
     3961 aaaaactcac aatccatttc aggaattggt gaaatcggtc ttgatagaac ctatacatca
     4021 agtgacgaac aatttgcaag acaaaaaaac gtctttacaa aattgctcga agaggctgag
     4081 aatctaaaaa agccagtttc cgtccactcg cgcagaacac tggatgatat ttttgaaata
     4141 attccatcgt acagaatatc tggatttttg ttgcactggt tcgatggaaa caaaaaacag
     4201 ctcaaaaggg caatggatct tggatgcttt gtatcatatg gaccactaac tgtgtactca
     4261 caagacaaac aagtgcttgt atcaaatacg gagcgggata aaattctagt tgaaactgac
     4321 ggccctgtca agttttcacg ttgttttggc atgaaggctg cacaaatcac ctttattcca
     4381 agtgtggttt tttgcgtctc aaaaatactt gggcagtcat acgacgagtg tgcttctgta
     4441 ttagaagcta attcgaaaaa ttatctggga atagtataaa ataccctaaa gttaacgtcg
     4501 agagtggata gaataaaacg actttcaatg ctggttcttg aaaatcacag agataaattt
     4561 acaacaaatt ttgcagaaaa caaaaaaact cttgatcagt tagtcatagt tcgctcaaaa
     4621 ggactaaaga acgaggtggc aggctacatt acaaaacttc tcaagcgcga agttgataca
     4681 aaaacaacaa cagaccaaga agaacaagaa gaccaagaag ttgaacaagt tgaagcgcaa
     4741 gaagtagtag aagcagaaga agagacagct gaagactcta caccacaaga aatcctagtg
     4801 gaaaatcaaa gccaggaatc aagctaatct aaaccgcaaa ttgattacaa tcaagttttt
     4861 gggtggtgca aaaaagtcat ttggtacggc tctgatctct gcagatcttg acaacgttgc
     4921 aattagggat ttgcttgcac atcttttatc aataaagcca aaaaacacac ttgatcttga
     4981 cacaaaaaat attcttgttg cagtaaatgg tatagattca tctgcccttg atgggcaaaa
     5041 cacggtctta cattctggcg acatcataag cattatacca ataattcatg gaggtactca
     5101 gagactccaa ttcaaaattt acaatacttg ggtagagttg ttcagtgtca catatgagag
     5161 aggtaaaaat tatgattttc ttgccttgac tcgcaagaaa tttccagacc taattatgga
     5221 aggcgtatcc tcaagatgca ttttgagcat tacgcatgca aaaaaaatca taggattatc
     5281 gctttttgca aaaaaacata acatactcct ctcaaaaaaa cttcaaacgg atattttact
     5341 gaggtttgcg gccacaactc agatatctga ggcaatcaaa gcggttggaa ttgaaggaca
     5401 cgatggcttt acaatcatcg ccatgggcaa aacgtcatct ttgaataaac ttgggagttt
     5461 tcttgcaaca catctggttt cgactgatta ccaaaaaaac ttcaaaccct tacaaaaaca
     5521 attcaaaatt tcaaaaaaac atttggacgc tgtagattca aaaacaccgc ttgaggattt
     5581 aatggttgaa aaggccgcag ttttattcca ctgactttct ttttaccctt tctacgctta
     5641 aaacatcggc atcttttagg actgacacgc cgctaattgc ccccaaaacc tctactaaaa
     5701 ttatccagtc gtgtttttcc aaagaaactt tgttttttac cttctctgca atttggctaa
     5761 ttatctcgct tgacgaaatg ccagaatttc ttttttctat tgttatgcga taagtttcat
     5821 ctggcttgat tttctgaatt tgattgagcg ctgcctctgt gattgactct acattggtgt
     5881 tgactgtttg aaaaatagga atgattcgga gtgtgtatct aattgaccat ggctcatctt
     5941 ctagtttttc atggattttt tttattattt cagctgaatt aacgctggtt tgaacagtca
     6001 aaatcccaga catatcagta atcgatactt gcgccttggg atccccaagc tcatctaaga
     6061 tgccggcaat ctcttctttt gtttcatcct caaaatgtct tgcacatgtg acaattagat
     6121 ccaaatgtga ttttggtgcg ccagctaagt attatagtgt ttgaaaacat gactcaaaaa
     6181 attaaatata gaaaacgatg tctagatttg cgttgagtgt agaacctgac atagaaaatg
     6241 attttgagga tgactttgaa gactctgaag aagaagaaat ggatgaagaa gatttcgatt
     6301 aaatggtcag gccgaaactg tctgcaaact tgttgaatgt gttgtaggct tgcaggaatt
     6361 ccctacctac attgctgatc ttgtatttgc atgcgccgct tgagttagtt tgctccaaaa
     6421 gtccttgcgc aaccaatgtg cctaacattt tagagattct gccatgagaa atgtttgcct
     6481 ttcgtatcag atatgtgatg cttgcaccat gttcgtctgg caagttatct cgagcggtgg
     6541 taagaatatc accaattatc tgcatatgcg ttctgtaggc tgacattgcc atttccggta
     6601 aagatgtgtt aacatagttc ttcaatatat tgtggggata aaaataattg acaaaataat
     6661 atcattggac tagatttacg catcatctgg ggtttcgtcg aacttaattt tagtcaacgg
     6721 aaccttggtt actggaacaa acaacattat caggccggcg attttcattg aaattgaaac
//
LOCUS       DASWKH010000058         6080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_143718, whole genome shotgun sequence.
ACCESSION   DASWKH010000058 DASWKH010000000
VERSION     DASWKH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143718"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGA92_04405"
     CDS             complement(<1..187)
                     /locus_tag="VGA92_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845686.1"
                     /translation="MDDEARNFQVAQIAELITNDKISLDEQDQKKLQKYIDYAKEEFQ
                     LSEDDSIQLVNETFLYLK"
     gene            complement(443..2578)
                     /locus_tag="VGA92_04410"
     CDS             complement(443..2578)
                     /locus_tag="VGA92_04410"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HEX9845687.1"
                     /translation="MKISTLVTLLLIILMAAVPNLGTLVAQAEKYVPPIVSTRGHAEE
                     SDIPKAPDPWLTKPRNALGYDVIGDVPGFNDKTSPKEIVIYVHGWNNNEDTAKKNFNL
                     FQEALGKKSKNLAKNVIGFSWDSNTGIGDYGDAKKIAKLNGPKLAQFILDYHSESENT
                     KIRIVGHSLGSQVILNAIQHLHNNQESTDKIITTVDFVGASVNDGAIAMTPGGFGKAI
                     SKVVGQLNNYYNRDDPALDLYYLVEKDQKALGDEGVKTKIILPPNYNQYDVTSKVKYS
                     HNAYNKAIVDIVNNWNISNSKKVENIDNKDLSNMQKNNEIDDLLAKGHKFRDSGDYGK
                     AITYYKKALLIDPKNFVALENLGNIYYNKIGNWVYAKKYYEQALLIDPKNSKISDYLS
                     RISGFNSEINYQENEQKNEQKNKEFNDLVSKGDNFRDKGQYHDAENYYKQALQINPAD
                     SNVLYSLGDIYEKLEDYENAIKYYNKALKINPNHIRAAFSAGYIYYFKIENLDQGRYY
                     YEMALKIDPKNSYVLDTLGEIYEDLGDYEKAMKYYDRALESDPNYKYAKEHKKALQEK
                     IKSPKISTPTEPQTAARDLTSNWEGTMTWSSVDLTEEFRPNNCEYSGKVSLILSQNGN
                     VLGGIVSYPELVESTHNEICNILTSGLTFSVINANVFGTGFSGTLGKLNADGQFTTDF
                     LRVEISGTLDVGGIPVNEIHGQFTTQRVN"
     gene            2888..3421
                     /locus_tag="VGA92_04415"
     CDS             2888..3421
                     /locus_tag="VGA92_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845688.1"
                     /translation="MNLYFVLKLVAYLNQRISEDEVLNAESPLISPNTGYRTFRGKNE
                     GKKFLSTARIEQDVREAMRPRFKWRPYVLRAFFDTQLIAESRGKIAHDFRVFFMGHKG
                     SIEAKYTANKSILPKVLTDEMYESFRKCQEFLDYETNTRQDEQITNQRVVTEEEFERL
                     VTEGWQYLEPRQTAKLS"
     gene            complement(3590..4528)
                     /locus_tag="VGA92_04420"
     CDS             complement(3590..4528)
                     /locus_tag="VGA92_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9845689.1"
                     /translation="MGRGPLQIIQETPQYYQLLKKYLGYKLFNKRSPIFGSVDIINVC
                     NLHCTHCYWWLNRKEDTELTVKDWKGIIDEKLKKQNVFVVTLVGGEPMMRPDIVELFV
                     KEFPKRACVVTNGTYPIPKYPGTYFYWVSVDGTQSVHDRIRGEGAWAQTRKNVLAYVE
                     KNGEKAWKDIWISMTVNSQNYHTIRDVVSEWYEHTNKIGFQFHTPFVKGDPLWLPFGP
                     ERNGVIDDILSLQKEYKDYIVNPPKNLELMRRSWGGKGTTPIDCPTWGIISLDHRGRE
                     KHPCCIGSAEKESLAPICEDCGLGCYSILLNSGLRG"
     gene            complement(4650..4853)
                     /locus_tag="VGA92_04425"
     CDS             complement(4650..4853)
                     /locus_tag="VGA92_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845690.1"
                     /translation="MKLYLEFEPCNECKDIIKELSANPLGLSIEDSRKFLRHVTYNHN
                     ETVQAIVKEFPKQEKQEKFDWFR"
     gene            complement(5029..5169)
                     /locus_tag="VGA92_04430"
     CDS             complement(5029..5169)
                     /locus_tag="VGA92_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845691.1"
                     /translation="MNAEWIVTGIILLGAMAAGGVSLYIKRRKASLADSSGNIQPDSN
                     EI"
     gene            complement(5166..5300)
                     /locus_tag="VGA92_04435"
     CDS             complement(5166..5300)
                     /locus_tag="VGA92_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845692.1"
                     /translation="MADKKKLNTSKDDYDKAVVSEDPTSTKMDLENLAKEAMKKFKSL
                     "
     gene            complement(5365..5820)
                     /locus_tag="VGA92_04440"
     CDS             complement(5365..5820)
                     /locus_tag="VGA92_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845693.1"
                     /translation="MNKKLLYTLPALAAVVALMFVVATPYVLAESGDNAWKMGKFHKH
                     HTIQVDGFVGSIQITEDTDRATLKDKVTVSLSEASQGLDVHMAKLGKVTNENGDKFLA
                     WTLVSVDKNEDTVTFTIHVVDAGDADNTAQITKEFDHSFKTEHDSDEKA"
BASE COUNT         1636 a         1237 c         1156 g         2051 t
ORIGIN      
        1 gctttaggta gaggaatgtt tcgttgacca gttgtatgga atcatcttct gataattgga
       61 attcttcttt ggcatagtcg atgtattttt gcagcttttt ttgatcttgt tcatctagtg
      121 agatcttgtc gtttgtgatt agctctgcta tttgcgcgac ttggaaatta cgggcttcat
      181 catccatgag attggtagaa tatgtgtggc ttttaagcga tttgagggcg agtccggggt
      241 tctcttacta acagttggta cgagcccgag aaattttgtg aacaaaagtt gatacaatgc
      301 gtgcagtaag cttatcggag tagctacaaa actcctttcc aaatttttta ttatcaatta
      361 acaaacgagt tattgacaat cggtaaaact acatccacga tttacgagta gaggagacat
      421 ctatgaaata attttaatat tttcagttta ctctttgtgt agtaaactgc ccatgtatct
      481 catttactgg aatacctccc acatctaatg tgccagatat ctccacccta aggaagtcag
      541 tcgtgaactg gccatcggca ttaagcttgc cgagagtgcc tgaaaatccg gttccaaaca
      601 catttgcatt tattacacta aatgtaagcc cagaagtcaa gatattgcag atttcattat
      661 gtgtggattc cacaagttct gggtaagata caatgccacc caaaacattt ccattttgag
      721 ataatatcaa cgaaactttt cccgagtact cacaattgtt tggcctaaat tcttcggtta
      781 gatccacact agaccacgtc atcgttccct cccaattact agtcaagtcg cgtgcagcag
      841 tttgaggctc tgttggtgtt gatatctttg gagattttat tttttcttgt aatgcttttt
      901 tatgttcttt tgcatacttg taatttggat ctgattcaag tgccctgtca taatatttca
      961 tggccttttc ataatcccca agatcttcat aaatctcacc aagagtatct aacacataag
     1021 aattcttggg gtcaatttta agcgccatct cgtaataata tctcccttga tctaaattct
     1081 caatcttaaa gtagtaaata tagccagcac tgaatgctgc tctaatatga ttggggttaa
     1141 ttttaagcgc cttgttgtaa tatttgatgg cattttcata atcttcaagc ttttcataaa
     1201 tatcgccaag actgtataat acgttggaat ccgcagggtt aatttgaagt gcctgcttgt
     1261 agtagttttc ggcatcatga tattgaccct tatctcggaa attatctcct ttactaacta
     1321 aatcgttaaa ttctttattt ttttgttcat ttttttgttc attttcttga taatttattt
     1381 cagaattaaa accagaaata cgggaaagat agtcggatat tttagaattc tttgggtcaa
     1441 ttaaaagtgc ctgctcataa tattttttgg cataaaccca attccctatt ttattatagt
     1501 aaatattgcc aagattttct aacgcaacaa aattctttgg gtcaattaaa agtgcttttt
     1561 tataatatgt gatggctttc ccgtaatcac cgctgtcacg gaatttatgc cctttagcaa
     1621 gtaagtcatc aatctcatta tttttttgca tattacttag atctttgtta tcaatatttt
     1681 cgactttttt agaattagaa atgttccaat tattaacaat atcaacaatt gccttattgt
     1741 aagcattgtg agaatatttg acttttgaag taacatcata ttggttataa tttggtggga
     1801 gaattatttt cgtcttcaca ccttcatctc caagagcttt ttgatccttt tctactaaat
     1861 aatacagatc cagcgcagga tcatcgcgat tataatagtt gttaagctga cctacaactt
     1921 tggatattgc tttgccaaag cccccaggcg tcatagctat ggcaccatca tttactgagg
     1981 ctccaacaaa atcaactgtc gtgataattt tatctgttga ttcctgattg ttatgcaagt
     2041 gttgaatagc attgagaata acctgagatc caagtgaatg acctacgatt cgaatttttg
     2101 tattttcaga ttcgctgtga taatctaaaa taaactgtgc aagtttggga ccatttagct
     2161 tggctatttt ctttgcgtct ccataatcac ctattcctgt attggaatcc cagctaaaac
     2221 caattacatt ttttgcaaga ttttttgatt tttttcctaa agcttcttga aacaagttaa
     2281 aattcttttt agctgtatct tcgttattat tccatccatg aacgtaaatt actatctcct
     2341 taggactagt tttatcgtta aaacccggaa catctccaat gacatcataa cctagtgcat
     2401 tccttggttt tgtaagccat ggatcagggg ccttgggaat gtcagattcc tctgcgtgtc
     2461 ctcgtgtgga aactataggt ggaacatatt tttctgcctg tgcaaccaag gtcccgagat
     2521 ttggaacggc tgccatcaaa ataattagta atagggttac caatgttgaa attttcaatg
     2581 atatgcttta gatgttgaat gtataaagtt gatcttttag ttttacgggt agtttgatat
     2641 tatatcctcc attccaaatt ttttattttg tatcattaaa ttttgatgct tcctttctgg
     2701 acaagggttt ctaattatgt aatcgcaatt ttctgtaatt ctctggtttt ttctactata
     2761 gccttacttt cattttctga tttttttatt tcttcaagtt tttcaagaat gttgttcttt
     2821 ttgttttgat caaatttgtt atattcttta acgagttttt cgagttgttc agagtaatta
     2881 gaaacgtatg aacctatatt ttgtattaaa actagttgca tatcttaacc agagaatcag
     2941 tgaagatgaa gttctaaacg cagaaagccc actgatatca ccaaatacag gatatcgcac
     3001 attccgtgga aaaaacgagg gaaagaaatt cctctcaact gcaagaatag aacaggacgt
     3061 cagagaagcc atgcgaccac gcttcaagtg gaggccatat gtgctaagag cattctttga
     3121 cacccaacta attgcagaat cacggggaaa gatagctcac gactttcgcg tgttctttat
     3181 gggtcacaag ggatccatag aagcaaaata cacagcaaac aagagcatac ttccaaaggt
     3241 tctgacagac gaaatgtacg agtcatttcg aaagtgtcag gagtttctgg actatgagac
     3301 caacacaaga caggacgagc agataacaaa ccagcgagtt gtcacagaag aagagtttga
     3361 aagactggta acggaaggct ggcagtacct ggaaccacgc caaacggcaa aattgtcata
     3421 aaaaatgacg gggttctgac taacagataa agcgggcccg ggggttctct tgccaacagt
     3481 cggtgcgagt ccgagaaatt ttgtgaacaa agcttgaaag ggcttttttc gcccattgga
     3541 aaaattacat gatatatttt tgccaaaaaa tcactaaagg cttgatcgac tagccgcgca
     3601 gaccagagtt caataaaatg gaataacacc caagaccgca gtcctcgcag attggcgcaa
     3661 gcgattcctt ctcagcactc ccaatgcagc acggatgctt ttcgcggccc cgatgatcca
     3721 atgatatgat tccccatgtg gggcagtcaa ttggtgtcgt gcctttgcca ccccagctcc
     3781 tcctcatcag ctccaagttt ttgggcggat ttacgatata gtccttgtac tctttttgca
     3841 aggataggat atcatcaatt acaccatttc gctcaggccc aaaggggagc cacaacggat
     3901 cgccttttac aaacggcgtg tggaactgaa acccaatctt gttggtgtgc tcgtaccact
     3961 cagataccac atctcgaatg gtgtggtagt tttgcgagtt tacagtcata gatatccaga
     4021 tgtccttcca ggccttttcg ccgttttttt ccacgtatgc cagaacgttt ttgcgtgtct
     4081 gcgcccaggc accctcgcct cgaatcctgt catgcactga ctgggttccg tcaactgaga
     4141 cccagtaaaa atacgtccca ggatactttg gtattgggta ggtgccatta gtcactacgc
     4201 aggccctctt tgggaactct tttacgaaca actctacaat gtctggccgc atcatcggct
     4261 cgcctccaac caaagttacc acaaagacgt tttgcttttt gagcttctca tcgattattc
     4321 ccttccagtc ctttactgtg agctcggtgt cttcttttcg attcagccac caataacagt
     4381 gggtgcagtg cagattgcac acattgatta tatcgactga gccaaaaatg gggctgcgct
     4441 tattaaatag cttgtagccc aggtattttt ttaataactg atagtactgg ggagtttcct
     4501 gtatgatttg cagaggaccc cttcccataa tttccactca gagcaccaca tgtaacaaag
     4561 cgcctttact ctacttaaac cagctcaaag ttttgccaaa agaccaaatg gattgtcgct
     4621 ctaattttcc cagctaatca aaaatctgat tatctaaacc agtcaaattt ctcttgcttt
     4681 tcttgctttg gaaattcctt tactattgct tggactgttt cgttgtggtt gtatgttaca
     4741 tgtcgtagga attttctaga atcctcgatg ctcaggccaa gtggatttgc actcagttcc
     4801 tttatgatat ccttgcattc gttgcacggc tcaaattcca aatagagttt catctgctga
     4861 tcatttacca tcattagtct atttaaatta gaaagtgtca tagtagaaag caagttaact
     4921 gttggtaagt ttcttttgtt tttgttgtga cattttgtct aacgtataat gattttgtca
     4981 gagtataacc tttgattaga tttggattta atcaatttac aaagacagct agatttcatt
     5041 ggaatctggc tggatgttcc cagacgaatc agccaaggat gcttttcttc ttttaatata
     5101 caggcttact ccacctgctg ccatcgcacc caaaagtatt atccctgtaa ctatccactc
     5161 ggcattcata gcgacttgaa ctttttcatg gcttcttttg ctaggttttc taaatccatt
     5221 ttggtactag tcggatcctc agagactacg gccttgtcat agtcgtcttt actggtgttt
     5281 agcttctttt tatcagccat gatacgtaat acacattgaa ttaattaagc tgctcggtaa
     5341 aaagttttga aaataaaaag aggtttaggc cttttcgtca gagtcgtgct ccgttttgaa
     5401 gctgtgatcg aactctttgg ttatctgcgc tgtgttgtct gcatctcctg catctaccac
     5461 atggatagta aatgtgacag tgtcttcgtt tttgtccact gagactagag tccatgccag
     5521 gaacttgtcg ccgttttcgt ttgttacttt gccaagcttt gccatgtgca catccaggcc
     5581 ttgagaagcc tcacttagtg atacggtcac tttgtctttt agagtggccc tgtcggtatc
     5641 ctcagtaatt tggatagagc ctacaaaccc gtcaacttgg attgtgtggt gcttgtgaaa
     5701 ttttcccatc ttccacgcat tgtcgccaga ctcggctaga acgtatggtg tggctacaac
     5761 aaacatcaac gcaacgactg ctgccagggc aggaagcgtg tatagtagtt ttttattcat
     5821 tagattcact tgtgaggttt gtgttaaaac cgttgtttat caatgatagg taactttgtt
     5881 accccttcat agactgctga ggggcacgta gttttttagc aaaaactaaa tcctatccga
     5941 ttcttccctt cagtactatt ttttcattgg tgcctgacgg ggtggatgag tccacatatg
     6001 tcatctcata ccagtttccg atgtgaattg tttttccatc tattgttcgc gggatcttga
     6061 tcaccacttc aaaggttcct
//
LOCUS       DASWKH010000059        20660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2018643, whole genome shotgun sequence.
ACCESSION   DASWKH010000059 DASWKH010000000
VERSION     DASWKH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 20660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20660
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018643"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="VGA92_04445"
     CDS             <1..1223
                     /locus_tag="VGA92_04445"
                     /inference="COORDINATES: protein motif:HMM:NF027718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HEX9845694.1"
                     /translation="GYFVENTMNGHLYRMSGSIKGSNLQTDLIGSFELVSSTNIYEKE
                     KPPPPPEPEPPQILLMTKQFSNAMIGGYYKFDLKVYYTDKNPFRDYYQLGGEAQNATV
                     SLKITSPTGVILEQFDGYTNSQGYFEDQFIIPKNTQPGQYAVSVNAQMGESMDNNALV
                     LSIVEQPRPDADVETVSGSPIITLIGDDPQTILKDSPYVEANATASDPEDGDLSGAII
                     IDSSGVDTSTVGTYVVTYTVTDSDGNTANKTRTVTVFTNIVDSDFNDNLDVDSGETVF
                     VTNGAEVNGNIIIDDGTLILEDGCMVNGNVYSENGGTINVTNCTITGSVKTKNSQSVS
                     IEDSTINGDIVSENDETLYITDTTIGGNVDVKDNQFVSITGNTINGNLNIQNTAGSCT
                     NTGNNVNGDIDGCP"
     gene            complement(1254..1475)
                     /locus_tag="VGA92_04450"
     CDS             complement(1254..1475)
                     /locus_tag="VGA92_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845695.1"
                     /translation="MVRIVSDEDARRVEMLQVISKKGLKVFNLEELKELQTLVEKKDY
                     SHNKKAHKSKVRLLAQINARIYDLEEKKL"
     gene            1546..2580
                     /locus_tag="VGA92_04455"
     CDS             1546..2580
                     /locus_tag="VGA92_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9845696.1"
                     /translation="MTDMRAMVLEKLAPIETRPLHLKEIAKHQIKNQNEVLIKIEACG
                     VCRSQLHGIEGDWQKYGLPPTLPTIPGHELVGTIIEIGNKVTKLKVGQRVGISPLLGS
                     CMQCGYCKEGKEHLCDLAEVTGESLLGGYAEYITASEDFATPIPDSMKSEYAAPLFCA
                     GITSYKAVVASEPAKGKKVGIFGVGGVGHLAVEFSKLKGCRVIGIARSKNHLDVAKKV
                     GADNVIQYSPDQAEFLSTLRREEGLLDSAIVFAPSESVTDAAINSVKKGGLVVVGTFG
                     NIPNFDVTHEKTIRGTLIGSRSDMKEVIRIAEQNQLQIIYKTFPLEQANEILADLKHS
                     KIDARAVLKP"
     gene            2606..2755
                     /locus_tag="VGA92_04460"
     CDS             2606..2755
                     /locus_tag="VGA92_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845697.1"
                     /translation="MNCRRCHHLSQAHESSNDSDSLMKLGKCSIPGCTCRQYLEFEQL
                     DEELL"
     gene            2794..3027
                     /locus_tag="VGA92_04465"
     CDS             2794..3027
                     /locus_tag="VGA92_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845698.1"
                     /translation="MEKHRSSDDMKKELDVMLAKLNALEIIAQDEFQKGTVKVLRRLV
                     EGQIHSVNEFDHLKKALDLITLQLFEVKNKQNS"
     gene            complement(3163..3483)
                     /locus_tag="VGA92_04470"
     CDS             complement(3163..3483)
                     /locus_tag="VGA92_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845699.1"
                     /translation="MTLQILSYEFLGPIRLSEWGPPMDQVLYLLLKRSNETFQILYAG
                     ESEKTDEADFFTKNEKFKCWIQNVGTENNLYLSIYPMWGSKQEERQRILKKIIDRFQP
                     PCNG"
     gene            3726..3980
                     /locus_tag="VGA92_04475"
     CDS             3726..3980
                     /locus_tag="VGA92_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845700.1"
                     /translation="MAEPHNYRTVGFSMILVAASLTVIALLYLAIGDDVLYSDRVGKQ
                     KQFEYQQFLEEAKQKFEQATESGGKNLSVDLNEDMNLQNP"
     gene            complement(3982..4671)
                     /locus_tag="VGA92_04480"
     CDS             complement(3982..4671)
                     /locus_tag="VGA92_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845701.1"
                     /translation="MYNWDEGLYEKRRFSFRNVVLFGSSAALAILLFLVFAPQIQELT
                     KTIPYLIELAYIPTVIVGFVFGLKITERAISPAETRSPIKRAIIKIFLFFFIIGGLFS
                     SVSFAFNGGIFLPDKPVLEMGLAEWMSQFTSQNGGLTFLIISSITIMAVATKRIIGLG
                     GIVNSISTFVGTFIFFSMVALSLSHTEPSTSQVFLYTFYHAGIIGGALFKMNKLTSNL
                     NLWEDFNNGYR"
     gene            4770..4976
                     /locus_tag="VGA92_04485"
     CDS             4770..4976
                     /locus_tag="VGA92_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845702.1"
                     /translation="MRISGALIPIGSILIIFGAIFQFQGRGAIGPESSVMYYNADWIY
                     YGIAIIVCGIIMSGVGGFFSIKKR"
     gene            complement(4973..5200)
                     /locus_tag="VGA92_04490"
     CDS             complement(4973..5200)
                     /locus_tag="VGA92_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9845703.1"
                     /translation="MVQTYILLSCSVGSEHEVLEQLRALAEIKDAIMTYGDYDIVAKI
                     ETGSEQQMDNLISSIRQLQKIRSTVTLHVTD"
     gene            complement(5280..5519)
                     /locus_tag="VGA92_04495"
     CDS             complement(5280..5519)
                     /locus_tag="VGA92_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845704.1"
                     /translation="MMNPIFIIGITFLVLAGALGGYVVYHKETVMTPLEMQEKTEIES
                     MSCEEVKVKHELGQYWSFTNWRIADAKVDACFPDQ"
     gene            5669..5920
                     /locus_tag="VGA92_04500"
     CDS             5669..5920
                     /locus_tag="VGA92_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845705.1"
                     /translation="MTTKLVIERGTATITVPKVYSIWSGQSFLEHQKKKMALLPEKFS
                     LTTKRGTSGLKMSEYSTVDKIPPKGLVSSTARGTASRIL"
     gene            complement(5926..7089)
                     /locus_tag="VGA92_04505"
     CDS             complement(5926..7089)
                     /locus_tag="VGA92_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9845706.1"
                     /translation="MNKEARGTAQVSIIIPTYNESQNILEVLKSIEEHLPANTTAQAI
                     VVDDNSPDGTGKLVEDYLQNVKKIANYTIDVIHRKTKEGLSSAILKGIQYATGNTIVV
                     MDSDLSHPPSLLPKMLDALKHPKCDIVIASRYVRGGSIDGWTLRRKLISKTATLIAKR
                     GLGINAKDPMSGFFAFKKQIIEGLKFDAIGYKMLLEILVKAKNISVLEIPYTFTNRKF
                     GSSKLDSKAITDYIKAVWKLYRFGKAAAREEKRTSVSFLSKAARFYTVGAIGLVINYL
                     ASSLFTDVVTHMWYIHATIIGIAISMSSNFVLNKVWTFEDRNFAPKYTLVQYSKFIGF
                     SSLGALAQLGMVFLLVDNYSIQYPIALVLAVFCAALSNFILNKKWTFKEKIWS"
     gene            complement(7168..8361)
                     /gene="truD"
                     /locus_tag="VGA92_04510"
     CDS             complement(7168..8361)
                     /gene="truD"
                     /locus_tag="VGA92_04510"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX9845707.1"
                     /translation="MIPKIDSDTGILSYTTGYPGCGGIIRTRPGDFAVSEVLDKKTIS
                     SLSDEGFAVYRLKKSGIDTNHALERIFKKSGVHLKALGLKDAAAQTEQFVCSMGKNKF
                     LPNYSDEKISLEKIGFAKKPLSAKDMVGNHFVIKITGATGDISSFSEQDKILNFYGYQ
                     RFGSKRPVTHLVGKALIQKRFGDAVSLILSYTSEYDSEENTKIRKMMSDISKYSEALQ
                     LVPKQMDLEIILLREMIAHANPQKALYKLPLSIRRLFVDAYQSYLFNLTLSKSYEYGE
                     ELFAPQQDDVCYDKNAKLGKYEMISHQNIAIPLIGYSYFKKTRFDYHISKILAEQEIS
                     PKNFYIKEMQEVSSEGGFRTARMVCTDYIAKKDTASFTLQRGSFATIIMREIMKPSDP
                     LRSGF"
     gene            complement(8358..9926)
                     /locus_tag="VGA92_04515"
     CDS             complement(8358..9926)
                     /locus_tag="VGA92_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX9845708.1"
                     /translation="MKKKVKGLIVAIFFGVILVTSTVAIALSGMPQQSPLDKVTITGT
                     PADNFPDLERPRFCETQEAKFTQYVREYKIPTECTQPLAITVDPQGNVWFAQTNTGMM
                     AKFDPNTEQFTEYENPDWPQMGRSMVWGIDYSYDGNVWYTDDTYDSIWKFSTTTGDYE
                     RVGFPSSEDSLPQHLKVAGNQIIVNDFYGGKISFFDTQQQGEEKLYRNVPSPIPDSFV
                     GGFDIDSYGNIWYTNWVFRQGGALVKFDYTQFSESGFSGDNVVASQFSEAFNLPSDLG
                     TPIGLSVDQNDNVWIADTSSSSFFKFDPISETFTKYVTSDPRPSAYGNATGVIKTPVT
                     GPYWTQVEDGKLIFNEQIANAIAVFDVDGEWLVEYQVPSQNPTWADCGSQSDCGIAQV
                     FGFRYAGDKIWFTEWVENNIGVVDLTQDLPFEFGLTQKQLTIPRGQTTQLDLQLVPVS
                     NTGVILSSKTTAAFYDLTVQITTTNLSLTETQVVPVSITASPSALTGTYKVLLSARTN
                     DVTVSQFVTVNITP"
     gene            complement(9966..10319)
                     /gene="pth2"
                     /locus_tag="VGA92_04520"
     CDS             complement(9966..10319)
                     /gene="pth2"
                     /locus_tag="VGA92_04520"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase Pth2"
                     /protein_id="HEX9845709.1"
                     /translation="MASIKQVIVVRADLGMGKGKIAAQVGHACVLGAENVRRSRPEWF
                     EKWWGGQEKIVLKVNSEKELEEVKRGAIELGLPWSEVTDAGHTQIAPGTFTCISIGPA
                     PEDLVDKITSNLKLL"
     gene            10409..11545
                     /locus_tag="VGA92_04525"
     CDS             10409..11545
                     /locus_tag="VGA92_04525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9845710.1"
                     /translation="MSFDVVVAGGSIAGLFCAREVAKAGHKVLVIEEDFEIGSPEHCG
                     GLVSASGLENLGIVPSAKTLDSQIHCATLSSPGGKKITLDAKKQNVVVINRREFDKQI
                     AHQAQKNGAEIRVKTSLKEINGNEAITTSGTINCKIIVDARGAFSLVQRDRTGTLQSA
                     QYEIYADWIQKGLVEVNFDAQKYPGFFSWIIPAANGIGKVGVAGKAINAMSALESFLE
                     KKGKHSIIRKVFAPIWVNGPIESFVTRNVVTVGDAAGQSKPTTAGGIYSCGVGGILAG
                     RAISKFLETGNYDSLKEYQKLWTEKFGKEFEKQLFARKMLERLDNQTIDKLFSSVTPE
                     MVADISDNEDFDFHTTALVKLLGLKGSLKVAQTIFGSEIRKLLS"
     gene            11596..11775
                     /locus_tag="VGA92_04530"
     CDS             11596..11775
                     /locus_tag="VGA92_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX9845711.1"
                     /translation="MSASLALPVCSCCNRHIMPNDKCVKFNCPECGSVLIWRCESCRE
                     AARPYTCQSCSFSGP"
     gene            11779..12060
                     /locus_tag="VGA92_04535"
     CDS             11779..12060
                     /locus_tag="VGA92_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HEX9845712.1"
                     /translation="MARLLLITKILPTGTEIDLDSLAKGISEKLGDGIKLEKYTKEPL
                     AFGLYYLKAQFDLDDREGQMDSLENSVRSVQGVSEFEVLNMSRRSVDVK"
     gene            12067..14238
                     /locus_tag="VGA92_04540"
     CDS             12067..14238
                     /locus_tag="VGA92_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX9845713.1"
                     /translation="MARKDEPLQMRVGEAKQRDVGKKRARVGPDAMDFLKVNPGDIIE
                     ITGKRASCAVVWPTDEDDKFADLVRIDGQTRKNISATLNDIVKIKKAPAKAAKSISLI
                     PVSDVVTVDKEFTDFVKNRLKGLPFCQGDEISVMILGNPMDFKIGKTAPKGIVTIDRT
                     TEFAISADKAIDKKVRITYDEVGGLRNELKIMREIVELPLRHPEIFNRLGIEPHSGIL
                     LYGPPGCGKTLIAKVLASESEANMFSINGPEIMNKYYGETEAKLREIFKEAKDNSPGI
                     IFIDEIDAIAPKREEAYGDVEKRVVAQLLALMDGLTDRGNVIVLGATNRPDSVDPALR
                     RPGRFDREIEVSVPNVDGRLEILQIHTRGMPIADDVELKRLSAELHGHTGADIKSLCR
                     EAALKSIRRYLPEIDLETEKVSAEILQSMEITLQDFYDAMHEVVPTAMREFYVERPKV
                     FWNDVGGLDDIKRTLQENIIVALKEPQKFHNMGIKPPRGALLYGPPGCGKSLLARAVA
                     TESGSNMILVRGPEILSKWVGESEKAIREIFRKAKTSAPCVVIFDEMDSLARMKSGEE
                     GGVGQTILSQILTEMEDSGSSRVVVIGITNRPDLLDNSLLRPGRLEPVLYVPQPDEKG
                     RLEIIKILTEKMPLSGDVNLQEISVSTQNYTGADLVALCREAGVSAMQNNAAKITSAN
                     FAAALKKIRPSITKEVDQWYLAIKETISNVVPKTIDKTFYG"
     gene            14244..14546
                     /locus_tag="VGA92_04545"
     CDS             14244..14546
                     /locus_tag="VGA92_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845714.1"
                     /translation="MAEFPIRNLVYEKIKSAGSLTDEELTRSLSKSGVTLAQDKFNKV
                     LLDLEILGLVKVSWLTKDTRRVEATVQEKEVDEYDEQNKEMAEKDYEASFPGLEES"
     gene            complement(14552..15355)
                     /locus_tag="VGA92_04550"
     CDS             complement(14552..15355)
                     /locus_tag="VGA92_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007648913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9845715.1"
                     /translation="MIIDCHTHLNQYESLANISSLDDRVRRLLRIMVSHNVDYSIILS
                     SYVVNEQRPSTQQLIEATKKHDNLGIVAGYSIDNHTDEDLRQYKKWIKDGLVKGLKLY
                     CGYEHYYPYDKKYQKVYDTCVEFGIPVMIHTGDTFSGKGKLRFSHPLNIDEVAVDNPE
                     LKIVMCHLGNPWIEDCQEVVYKNVNVYADMSGLAVGKFDHFFENMMVEKVAELINYAG
                     EPRYLLYGTDWPISNMDSYLSFVAKLKLKKDYRDKLMYKNAKDLFKISV"
     gene            complement(15357..15998)
                     /locus_tag="VGA92_04555"
     CDS             complement(15357..15998)
                     /locus_tag="VGA92_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9845716.1"
                     /translation="MNDKTKIKIRHTTHEDIPKIVELQNVSFPFMALEGMIWKPEQLQ
                     RHLEVFPEGQFCADCNGQIVGSVSSLIVNLEPEYQEHTWMGICGGPRFGNHNPSGDSL
                     YGADVSTHPDYRRLKVATRLYDERKNLCIKLNLRRIIAGGRLFNYCEFAEKFSAEEYV
                     NKVIEGDIDEPVLGFQLKNGFKFIKILPNYLKDSRSQNYATFIEWKNPHYKVN"
     gene            complement(16082..17200)
                     /locus_tag="VGA92_04560"
     CDS             complement(16082..17200)
                     /locus_tag="VGA92_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845717.1"
                     /translation="MRKFGAASLFLMSVLLAGVITPATNGFQMIGFVPTNTYVDFAFA
                     QTDDSENDIEDETVDDVEDQTQEETEDEIMDDAEDEIEEETETDNLGREVADFVHNTR
                     DLFREQREETRAIIDACRDAVRDAAPEERADIRMQCREDLDEVRESYRQLRATYQDVF
                     REFRDHVRVLVRDAQGQSDADVMSAVDAINMRAQREDMRDRAHEIRMQMQEEFRAEVS
                     DLRDQMREDREMMREEIQKIREEGQDREELESIREQMREEQEKMREQMQEEREKLREQ
                     MQDEREKMQEDREKLREQMQDEREKMQEDREKMREQMQEEREQMTESEESEEIAAESI
                     EEAEETSEESEVTEESEETIEESEEPVEESEETEETQN"
     gene            complement(17280..17591)
                     /locus_tag="VGA92_04565"
     CDS             complement(17280..17591)
                     /locus_tag="VGA92_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845718.1"
                     /translation="MLALKKVKKFGVCEMTGYCIGTCVRYKARKPLEIGRYAAGQKRC
                     NLCEIFINSDGAFCPCCKRKLRSLPRSRKGKEKYMMQKSGLTITEDQKSTAQFCSLAE
                     S"
     gene            17888..18202
                     /locus_tag="VGA92_04570"
     CDS             17888..18202
                     /locus_tag="VGA92_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845719.1"
                     /translation="MKVLGDRKDFDLVFSRLNKNSALFKDVKDIIDNLKNDVIVGERI
                     KFEQIPLYYKKRHGVDNAFHVYLPEGMRLIYSITNYQGQKTAFLMELTGHKNYERRFK
                     YR"
     gene            18262..18609
                     /locus_tag="VGA92_04575"
     CDS             18262..18609
                     /locus_tag="VGA92_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845720.1"
                     /translation="MPARVFNKSKKNAPQNNTSGNIAIMNYILEGTRKFNPTLRSLTI
                     VEKLICENKEFDSKSRLYKKLPTGMQYPVFNFILKILERQGKIMLGSDGAIFWTGPAS
                     PKLQRSLKKAVEY"
     gene            complement(18651..19538)
                     /gene="cyoE"
                     /locus_tag="VGA92_04580"
     CDS             complement(18651..19538)
                     /gene="cyoE"
                     /locus_tag="VGA92_04580"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9845721.1"
                     /translation="MAAKEILEVSKPRIIVLLVITAVTSMYAASKLVGPALDPMGLVH
                     IIIAGALASAGSSALNHYYDRDIDPLMKRTSTRPIPSGRIKASSVLAYGLAVSVISVL
                     YGAIFLNLTSTFFIALGIFFYVIVYTVWLKRKNSSNIVIGGFAGSAASMAGWTAATGF
                     VPPVGIDLLGFLVGFLVFVWTPSHFWCLAMKIREDYAEAHVPMLPVLIGMQKTSKYIL
                     ANTAILLPYSLALYAFGLGIVYVAVAAASGGLMLAYHYKLTKTPTSDFAWKAYKVTAP
                     YLTIIFVAIALDAAFHFRF"
     gene            19631..>20660
                     /locus_tag="VGA92_04585"
     CDS             19631..>20660
                     /locus_tag="VGA92_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX9845722.1"
                     /translation="MSNWDLMMPGMGLTAIGLTGVTIAYAGIAHTFIDGMHALTGLTM
                     FIGLIFLSAGILEGGVSTSNRAKATTLVVLGISFSFGLAALTFNAITTIPTFAGVMLI
                     IAVPAIMMAYVAMKMPQYFKPISIIFVLATGAAVISYVGFGLYGPSQYLVPPPVEEEP
                     EVPVPTAPIFAISILPGSSISGNPDYSPDVAQVPHGNIIEWTNNDDVVHTVTSIVDAD
                     YTFDSVVIDPGESYQIDTSTLSPGTYEYYCMVHPWMTASFVLGEGAPAAPEFAISILA
                     DSSIQGNPDYSPDFAQVPLGDVIVWTNDDNTIHTATSTADPSLFDSDFLSAGDSYRLD
                     TTTLGPGTY"
BASE COUNT         5877 a         4604 c         4464 g         5715 t
ORIGIN      
        1 ttgggtattt tgtagaaaac acgatgaatg gacatctcta cagaatgtca ggcagtatca
       61 agggaagcaa tctccagaca gacttgattg gaagttttga gcttgtcagc tccacaaaca
      121 tttatgaaaa agaaaaacca cctcctcccc ccgaaccaga accgccacaa attctgttaa
      181 tgacaaaaca gttctcaaat gcaatgatag gcggttacta caagtttgac ttgaaggtgt
      241 actatactga taagaatccc tttagggatt actaccagct tggtggcgag gctcaaaatg
      301 ctactgtgag cctaaagata actagcccca caggagtcat tcttgagcag tttgacggct
      361 atacgaacag tcaaggctat tttgaggacc agttcataat tccaaaaaac acccagcctg
      421 gccaatacgc agtatcggtg aatgcacaga tgggagaaag tatggataac aacgctctcg
      481 tcctatcaat agttgagcag ccaaggccag acgcggatgt agaaacagtt tctggaagtc
      541 ccatcatcac attaattggg gatgaccctc agacaatact caaagattca ccgtacgttg
      601 aggcaaatgc cactgcctca gacccagaag acggcgatct gagcggtgca attattattg
      661 attctagcgg tgtggatact tctactgttg gaacatatgt cgtaacgtac acagttaccg
      721 actcggatgg aaacactgcc aacaagacaa ggactgtcac agtatttacc aatatcgttg
      781 actccgactt taatgataat cttgatgttg attcaggcga aacggtattt gtgacaaacg
      841 gtgcagaggt gaatggcaac atcataattg atgatggaac cttaattctt gaagacggct
      901 gtatggtaaa cggaaatgtg tattctgaaa atggtggaac gatcaacgta acaaactgta
      961 caattacagg aagtgtaaaa accaaaaaca gccaaagcgt ttccattgaa gacagtacta
     1021 taaacggcga cattgtatca gaaaatgatg aaacgttata cattacagac actacgattg
     1081 gcggtaatgt tgatgttaaa gataatcaat tcgtaagcat tacaggaaat accataaacg
     1141 ggaatctaaa catccaaaat actgcaggtt catgcaccaa tacaggtaac aacgttaatg
     1201 gcgacattga tggttgccca taatttcaga aagtagcaac acagtaataa tactcagagt
     1261 tttttttctt caagatcata gattctggca ttgatttgag ccaaaagcct cacctttgat
     1321 ttgtgcgcct ttttgttgtg tgaatagtcc tttttttcta ccagagtctg cagctccttt
     1381 agctcctcaa gattgaagac cttgagtcct ttttttgata tcacttggag catctcgact
     1441 cgcctagcat cttcatcgct tactatgcgc accacaaaat ggatcaattt tacatgcgtt
     1501 aaaatttttt gtaacacaaa aataagatga gatgttttgg caggtatgac agatatgcgc
     1561 gcaatggttc ttgagaagct agccccaatc gagacaaggc cgctccacct aaaggagatt
     1621 gcaaaacacc agatcaaaaa ccaaaacgag gttctcataa aaatagaagc ctgcggtgtc
     1681 tgccgctcgc agctgcacgg aatagaggga gactggcaaa aatacggcct gccacccacc
     1741 cttccaacaa ttccaggcca cgagctagtc ggcacaatca tcgagatagg aaacaaagta
     1801 acaaagctca aagtaggaca aagagtggga atttcacctc tgctcggctc ctgcatgcag
     1861 tgcggttact gcaaggaggg aaaagagcat ctctgtgatt tggcagaagt taccggcgaa
     1921 tcactgcttg gtggatatgc agaatacatt actgcctcag aggattttgc aactccaatt
     1981 ccagactcga tgaaatccga atatgccgca ccgctttttt gcgcaggaat cacatcatac
     2041 aaggcagtag tcgcatcaga gccagccaaa ggaaaaaaag tcggaatctt tggcgttggt
     2101 ggcgtagggc acctagcagt agagttttcc aagctgaaag gatgccgagt catcgggatt
     2161 gcaagaagca aaaatcatct tgatgttgca aaaaaagtag gagcggataa tgtaatccag
     2221 tattcccctg atcaggccga gtttctatca acgttaagaa gagaggaggg tttgttagat
     2281 tctgcaattg tttttgcccc atctgaatct gtgacggatg cagcaattaa ttccgtcaaa
     2341 aagggaggac tggtagtggt cggaacgttt ggaaacattc ctaactttga tgtcacacat
     2401 gaaaagacaa tccgcggcac tctgattggc tcaaggtcag atatgaaaga agtgataaga
     2461 attgcagaac aaaaccagct tcaaatcatc tacaagacat ttccattgga gcaagcaaac
     2521 gagattttag cagacctcaa acactccaaa atagatgcgc gagctgtgct taaaccataa
     2581 ataaaagaga tttttttcaa gaacaatgaa ctgcagacgt tgccaccatt tatcgcaagc
     2641 acacgaatca tcaaatgaca gcgattcttt gatgaagctt ggcaagtgct caattcctgg
     2701 ctgcacctgc agacagtacc tagagtttga gcagctagac gaagaattgt tgtaatttta
     2761 ggttcatata gcaatcagtc aacaaccaca cagatggaaa agcaccgttc ctcagacgat
     2821 atgaaaaaag aactcgatgt catgttagca aagctaaatg ccttggagat aattgcacag
     2881 gatgagttcc aaaaaggcac agtcaaggta ctaagaaggc tagttgaagg gcagattcac
     2941 tccgtcaacg agtttgacca cctaaaaaaa gcgctggacc tcattactct acaactgttc
     3001 gaagtaaaaa acaagcaaaa ttcatgattt tagcacggtg tctgaaaggc cgcattctgt
     3061 acatttcatt ataacacttg attcaagttc ctgagatttt tccatttttg cgccacaaca
     3121 cgggcagttc atggaaatca ttggtttgat tctttattag aatcatccat tgcagggcgg
     3181 ctggaacctg tcgatgattt ttttcaaaat cctttgcctc tcttcttgtt ttgagcccca
     3241 cattggataa attgaaagat acaggttgtt ctcagttcct acgttttgaa tccagcactt
     3301 gaatttctca ttttttgtaa aaaagtcggc ctcgtcggtt ttttccgact ctccggcata
     3361 aagtatctgg aaggtctcgt tgctacgctt tagcaaaaga tacaggactt gatccatcgg
     3421 aggcccccac tcggaaagcc ggattgggcc cagaaactcg tagcttagga tctgtaatgt
     3481 cacaaaaatg ctacgcgttg ctaacttttt tctttttttg tatcagagat atagcaaagc
     3541 aaaaatctga aggaaaagcg gagcaactgc ctccggagag ggtaccgcaa gaccttcagg
     3601 aaatattggt tatgatacca agtccttaag ctcctgtatg tgtattttga aacacaaatt
     3661 gtgatcgtaa attgaacgaa tatgcgctta aaggattaaa tcctactcac aatctggatt
     3721 agctgatggc agagccccac aattacagaa cggtaggatt ttccatgata ctggtggctg
     3781 cgtcgctcac agtaattgct ctgctttatt tggccattgg cgacgatgtg ctttattccg
     3841 accgcgtagg aaagcaaaaa cagttcgagt accagcagtt cctagaagaa gcaaagcaaa
     3901 agttcgagca ggccaccgag tctggtggaa aaaacctctc agttgaccta aacgaagaca
     3961 tgaacttgca gaatccctag attacctata cccgttgtta aagtcttccc agagatttag
     4021 attcgatgtg agcttgttca tcttgaataa tgccccaccg attattcctg cgtgataaaa
     4081 cgtatacaaa aagacctgcg atgttgatgg ctccgtatgt gagaggctca acgcaaccat
     4141 tgagaaaaag atgaatgttc caacaaatgt cgagatgctg tttactatac ccccaagccc
     4201 tattatgcgc ttagtggcca ccgccatgat tgttatgcta gagattatca gaaacgtcaa
     4261 gccgccgttt tgcgacgtaa actggctcat ccactctgct agccccatct caagtaccgg
     4321 tttgtctggc aaaaatattc caccgttaaa cgcaaagctg acagagctga acaaacctcc
     4381 aattatgaag aagaataaaa atatcttaat tattgcccgc ttaatcgggc tgcgcgtctc
     4441 agcagggctg attgcccgtt ccgttatttt gagcccaaag acaaacccta caatcacagt
     4501 cgggatgtat gccagctcaa tcaggtacgg gatggtttta gtcagttcct ggatttgtgg
     4561 ggcaaagacc aaaaacaaga gtatggcaag tgcggctgag ctgccaaaaa gcaccacatt
     4621 acggaaggaa aagcggcgct tctcgtaaag accttcatcc caattgtaca ttttctgaaa
     4681 tgttggaaat tagattaaag aaaaagacgg aaaatcattt gttcaattaa tcaattctct
     4741 ttatacacct gagcgcgcca catcagacta tgagaatatc tggagcactc atcccaatag
     4801 gctccattct gatcattttt ggggccatct ttcagtttca gggaagggga gcaattggcc
     4861 cagagtcgtc agttatgtac tataatgcag actggattta ctacggaatt gccatcatag
     4921 tttgcggaat cataatgagc ggtgttggcg gttttttctc gataaaaaaa cgctagtcag
     4981 taacatgtaa ggttactgtg cttctgattt tttgcaactg tctaattgag gatatcagat
     5041 tatccatttg ctgctcagaa ccagtctcta tttttgccac aatgtcatag tcgccatacg
     5101 tcataatcgc atccttgatt tctgctagtg cccttagctg ttcaagcact tcgtgctctg
     5161 agcccacgct gcagcttaga agaatgtatg tttgtaccat tttcccacat ctgtgagttt
     5221 atcgcaatca aaatccatag gcctaagaca aaaagccaaa aataaaaatc atcacataat
     5281 tattgatcag gaaaacaggc gtctactttg gcgtcggcaa ttctccagtt agtaaaagac
     5341 cagtactggc caagctcgtg ctttactttg acttcctcgc agctcatgct ctcgatttct
     5401 gtcttttcct gcatctcaag tggagtcatg acggtttctt tgtggtatac cacgtagccg
     5461 ccaagtgcgc cagcaagtac caaaaacgtt attcctatga tgaagattgg attcatcatc
     5521 ggttaggcgt tgatttggta ggtattttat ctttatgaca gaaattaatg caaaacaagt
     5581 ttgatgaact aacagatcag ctgataaatc ataacattag ctgagcagac aatatcagtt
     5641 ctgcagacat atggaaaaca gagatagaat gactacaaaa ctagtaatcg aaaggggcac
     5701 tgcgactatc acagtgccaa aggtctactc gatctggtca ggccaatcct ttttggagca
     5761 tcaaaaaaag aaaatggctt tgcttccaga aaaattctct ctgacgacaa aaaggggcac
     5821 ctctggcctc aagatgtctg agtattccac agtagacaag attccaccca agggactcgt
     5881 gagtagtacc gcaagaggca ctgcatcccg aatcctttaa tttttttagc tccagatttt
     5941 ttctttgaat gtccactttt tgttcagaat gaagttgctc aaggctgcgc aaaatactgc
     6001 cagcactagt gcaataggat actgtattga ataattgtcc acaagcagga aaaccatgcc
     6061 cagttgtgca agcgcgccaa gggagctgaa cccaatgaat tttgagtatt ggactagtgt
     6121 gtactttgga gcaaagttcc ggtcctcaaa ggtccagacc ttgtttagta caaagttgct
     6181 gctcatcgat atggcaattc caattattgt ggcgtgaatg taccacatgt gcgttacaac
     6241 atcagtaaac aaagacgacg ccagataatt aattacaagt cctattgcac caacagtata
     6301 gaatctggca gcctttgaga gaaagctcac agaggttcgt ttttcttccc ttgccgctgc
     6361 cttgccaaac ctgtagagtt tccagactgc cttgatgtaa tcagttattg cctttgagtc
     6421 aagcttgctt gagccgaact ttctgtttgt aaatgtgtaa ggaatttcaa gtacgctgat
     6481 gttttttgcc tttactagaa tttctaagag catcttgtag cctattgcgt caaacttgag
     6541 gccctcaatg atttgttttt tgaatgcgaa aaatccagac attgggtctt ttgcgttgat
     6601 tccaagaccc cgctttgcaa ttagtgtggc agtcttgctg attaattttc ggcgcagtgt
     6661 ccacccgtca atggagccac cccgcacata tcttgacgca atcacaatgt cacactttgg
     6721 gtgctttagc gcatcaagca tttttggcaa aaggcttggc gggtgtgaaa ggtcgctatc
     6781 catcacaact atggtgtttc cagtagcgta ctggattcct tttagaatcg cagagctcag
     6841 accctccttt gtctttctgt gaataacatc aatggtgtaa ttggcaattt tttttacatt
     6901 ctgcagatag tcttcaacta gttttcctgt gccgtctggc gagttatcat ctactactat
     6961 tgcctgtgca gtcgtgtttg caggcaggtg ttcctcaatt gattttagaa cctccagtat
     7021 gttttgagac tcgttatacg ttggaatgat tatagatacc tgtgcggttc ccctagcctc
     7081 cttgttcaca cttgactttg ccaaaaggtg tagatattaa ttttagtgaa tggagtatga
     7141 ctaaaggtgt aggtctgcta acagatctta aaatcctgat ctgagcggat cagatggttt
     7201 cattatttct cgcatgataa tggttgcaaa tgaacctctt tgcagagtaa agcttgcagt
     7261 gtcttttttt gcaatatagt cagtacaaac catacgtgca gttctaaatc caccctcgct
     7321 gctcacctcc tgcatttcct ttatgtaaaa attctttgga gagatctctt gttctgccag
     7381 aatttttgag atatgatagt cgaatctcgt ttttttaaag taagaatacc caatcagtgg
     7441 aattgcaatg ttttggtgcg atatcatttc gtatttccca agctttgcgt ttttgtcata
     7501 acacacgtcg tcttgttgcg gcgcaaataa ttcttcccca tattcgtacg acttgctcag
     7561 ggtaagattg aacaggtatg actggtatgc gtcaacaaac agcctcctaa tcgacagcgg
     7621 cagcttgtat aacgcctttt gcgggtttgc gtgcgctatc atttctctga gcaggattat
     7681 ttctaggtcc atctgttttg gcactagctg taatgcttct gagtattttg agatgtctga
     7741 catcattttc ctaatcttgg tgttttcttc cgagtcgtat tctgatgtgt atgagaggat
     7801 caaacttact gcatcgccaa atcttttttg gattaacgcc ttgccgacaa ggtgtgtcac
     7861 cggcctcttt gagccaaagc gctggtatcc ataaaaattc aagattttgt cctgctctga
     7921 aaagctggaa atgtcgcctg ttgccccagt tatttttatg acaaagtgat ttcccaccat
     7981 gtcctttgca gaaagcggct tttttgcaaa cccgattttc tcaagtgaga ttttctcatc
     8041 ggaataattt ggcaaaaact tgttttttcc catcgagcat acaaactgct cagtctgtgc
     8101 tgccgcatcc ttgagcccaa gggcctttag gtgcacgcca gattttttga agattcgttc
     8161 cagcgcatga ttcgtatcta ttccggattt tttcagccgg tatactgcaa agccttcatc
     8221 cgataatgac gaaattgttt ttttatcaag gacttcggag actgcaaagt caccagggcg
     8281 tgtgcgtatt attccaccac agccaggata gcctgtagtg taagatagga ttccagtatc
     8341 agagtctatt tttggaatca cggagttata ttcacggtga caaattgtga aacggttacg
     8401 tcgtttgttc ttgccgagag taaaaccttg tatgttccag tcagtgctga tgggcttgca
     8461 gtaattgata ctgggacgac ctgcgtctca gtcagagaca gattagtagt cgtgatttgt
     8521 actgtcaggt cataaaatgc agcagttgtc tttgatgaga gaattacgcc ggtgtttgag
     8581 actggaacaa gttgcaagtc tagctgcgtt gtctgaccac gtggaatggt tagttgtttt
     8641 tgcgtaagcc caaactcaaa tggaagatcc tgtgtcaggt ccactacgcc aatgttgttt
     8701 tcgacccact cagtaaacca gattttatca cctgcatatc tgaacccaaa tacctgtgca
     8761 attccgcagt cgctttggct tccacagtca gcccatgtgg gattctgcga tgggacttgg
     8821 tattctacga gccattcccc atctacgtca aatactgcta ttgcgtttgc aatttgctca
     8881 ttgaagatta gctttccgtc ctctacttgg gtccagtacg gacctgtgac aggtgttttg
     8941 attacaccag ttgcgttgcc atatgccgac ggacgaggat ctgatgtcac gtattttgta
     9001 aacgtttcag atattgggtc aaacttgaaa aacgagctgc tcgaagtatc agcaatccag
     9061 acattgtcgt tttgatcaac gctaaggcct atcggtgttc caaggtccga tggaaggttg
     9121 aatgcttcag aaaattgtga tgcaacaaca ttgtcgccag aaaaaccaga ttctgagaat
     9181 tgtgtgtaat caaacttgac tagtgccccg ccctgtcgaa atacccagtt tgtgtaccag
     9241 atgtttccat acgagtcaat atcaaaccct ccaacaaatg aatccggtat tggtgaagga
     9301 acatttctgt atagtttttc ctcgccttgc tgctgcgtat caaaaaagct gattttacct
     9361 ccataaaaat catttactat aatctggttt cctgctactt tgagatgttg aggcaacgag
     9421 tcctcacttg atggaaaacc aactcgttca tagtcaccag ttgttgtaga aaacttccaa
     9481 atggaatcat acgtatcatc agtataccag acgttgccgt catacgagta atcaatgccc
     9541 cagaccatcg agcgacccat ctgaggccag tccgggtttt catattcagt aaattgctct
     9601 gtgtttggat caaattttgc catcatgcct gtgttggtct gagcaaacca gacgtttcct
     9661 tgcgggtcga cagtaatggc aagaggctgg gtgcactctg tcggtatttt gtattctctt
     9721 acatattgcg tgaacttggc ttcttgtgtt tcgcaaaatc tcggcctttc taggtctgga
     9781 aagttatctg caggtgtacc agtgatagtg actttgtcaa gtgggctttg ttgcggcatg
     9841 ccactgagtg cgattgccac agttgatgtg accaaaatta cgccaaaaaa tattgcaacg
     9901 atcagtcctt ttactttctt tttcacagat gtgagtccag cttaccacat tatatttatt
     9961 gagatttaca gtaatttgag attacttgtg attttgtcaa ctaggtcttc tggtgcagga
    10021 ccaattgaaa tgcaagtaaa tgtccccggc gcaatttgcg tgtgacctgc atctgttacc
    10081 tcagaccacg gaaggccaag ctcgattgcg cctcttttta cttcctccag ctctttttca
    10141 ctgttgactt tgagaacaat tttttcctgg ccgccccacc atttttcaaa ccattctggg
    10201 cgagacctgc ggacattttc tgcaccaagc acacatgcgt gtccgacctg agctgcaatc
    10261 ttgccttttc ccatgccaag gtctgctcga accacgatta cctgttttat tgacgccaac
    10321 agttagagtg agcaaacaac cattgaaaaa cttttgaggg tgtaaccgac tctatgaatt
    10381 aaattaacgc acacacaaaa ataacgaaat gtcctttgat gttgtagttg ccggaggcag
    10441 catagcaggc cttttttgcg caagggaagt cgccaaggca ggccacaaag ttctggtaat
    10501 agaagaagac tttgagatag gctcccctga gcactgtgga ggtcttgtct cagcgtcagg
    10561 cctcgaaaac ctaggaatag tgccatcagc taagaccctt gatagccaga tccactgcgc
    10621 caccctcagc tcgcccggcg gaaaaaaaat aacacttgat gcaaaaaagc aaaacgtcgt
    10681 agtcataaac agacgcgagt ttgacaagca gatagcgcac caagcccaaa aaaacggagc
    10741 agagatcagg gtaaagacat cacttaagga gatcaacggt aatgaggcaa tcacaacgtc
    10801 tggcaccatt aactgcaaga taattgtgga tgcgcgtggc gcattttctc tagtacaaag
    10861 ggacaggacc ggcaccctac agtcggccca gtacgagatt tatgccgact ggatacaaaa
    10921 aggactagtc gaggtaaact ttgatgcgca aaagtatccg ggattttttt cctggataat
    10981 tccggcagct aatggaatcg gaaaggttgg agtagcagga aaagcaatca atgcaatgtc
    11041 tgcacttgaa tcattccttg agaaaaaagg caagcactct ataattcgaa aggtctttgc
    11101 tccaatctgg gtaaacggcc caattgagag ttttgttaca cgaaacgtcg taactgtcgg
    11161 cgatgcagcg ggacagtcaa agccaaccac ggcgggtggg atttattcct gcggtgttgg
    11221 cggaatccta gcgggccgtg ccatctcaaa atttttagag actggaaatt acgactcgct
    11281 aaaagaatac cagaaactct ggacggaaaa atttggaaaa gaatttgaaa aacaattgtt
    11341 tgcaagaaag atgttagaaa gactcgacaa ccagacaatc gacaaattgt tctcatcagt
    11401 aacaccggaa atggttgcag atatttcaga taatgaggac tttgattttc acacaactgc
    11461 cctagtaaaa ctgcttggcc tcaaaggctc actcaaagta gcgcagacaa tttttggttc
    11521 agaaatacga aagcttctca gctagtccga aatgaactgg gcaagctata aattgattat
    11581 taaaaaagac agctcatgtc agcatcactg gcacttccag tttgcagttg ttgtaacagg
    11641 cacatcatgc caaacgacaa gtgtgtcaag ttcaactgtc ctgaatgcgg atccgttctg
    11701 atttggagat gtgaaagctg cagagaggca gcaagaccat acacatgtca gtcttgtagc
    11761 ttttcaggac catagaaaat ggcacgactg ctattaatca caaagatcct cccaacaggg
    11821 actgagatag atctggacag cctagcaaag ggaatctcag aaaaactagg cgacggaata
    11881 aagcttgaaa aatacacaaa agagccatta gcttttggcc tatactatct aaaggcacag
    11941 tttgatttgg atgacaggga agggcagatg gactcactag agaattccgt tagatccgta
    12001 caaggagtaa gcgagtttga ggtcctcaac atgagccgca ggtcagtaga tgtgaagtga
    12061 ttagtcgtgg cacgaaaaga tgaaccacta caaatgcgcg ttggcgaggc aaagcaacgc
    12121 gacgtaggca aaaaacgcgc cagagtaggg cccgacgcaa tggactttct taaggtcaac
    12181 ccaggagaca taatcgaaat aacaggaaag cgcgccagct gcgcagttgt atggccgaca
    12241 gatgaggacg acaagttcgc agacctagta agaattgatg gccagacaag aaaaaacatc
    12301 agcgccacat taaacgacat tgtaaaaata aaaaaggcac ccgccaaggc cgcaaaatca
    12361 atttcactaa taccggtaag cgacgtcgta accgttgaca aggaatttac cgattttgta
    12421 aagaaccgcc taaagggcct gccgttttgt cagggcgatg aaatatccgt tatgatccta
    12481 ggcaacccca tggactttaa gattggcaag actgcgccaa agggcatagt cacaattgat
    12541 aggacaacag agtttgcaat atctgcagac aaggcaattg acaaaaaggt tcgaataaca
    12601 tacgacgagg taggagggct cagaaacgag ctcaaaataa tgcgagagat agtggaactt
    12661 ccgctacgac atccagagat attcaaccgg cttggaatcg agccgcacag tggcatcctg
    12721 ctgtatggcc cccctgggtg tggcaagacc ctgattgcaa aggtcctagc aagcgagtcg
    12781 gaggcaaaca tgttttcaat taacgggccg gaaattatga acaagtatta cggagaaact
    12841 gaggcaaagc tccgtgaaat cttcaaggag gcaaaggaca actcgccagg aataatattt
    12901 atcgacgaaa tagacgcaat tgcgccaaaa agggaagaag cgtacggcga cgtggaaaaa
    12961 agagtcgtag cgcagctttt ggcattaatg gacgggctca cagacagggg aaacgtaata
    13021 gtccttggcg caacaaacag gccagacagt gtcgacccag cccttcgaag accgggaaga
    13081 ttcgacaggg aaatcgaagt ctccgttcca aacgtcgatg gcagactgga gattttacaa
    13141 atccacacgc ggggaatgcc aatagcagac gatgtggagc taaaaagact ctcagcggaa
    13201 ctgcacggac atacgggtgc agacatcaag tctctctgca gggaggcagc actaaaatca
    13261 atacgacgat acctgccaga aattgatctg gaaacagaaa aggtctcagc agaaatcctg
    13321 cagtcaatgg agataacgct tcaggacttt tacgatgcaa tgcacgaagt cgtccccacc
    13381 gcgatgaggg agttttacgt ggagcgaccc aaagtgtttt ggaatgatgt gggagggcta
    13441 gacgacataa agcggacctt gcaggaaaac atcattgtag cactaaaaga gccgcagaaa
    13501 tttcacaaca tgggaatcaa gcctccgcgt ggcgccttat tgtacggccc accgggatgc
    13561 ggcaagtcgc ttttggcgcg cgcagtagca acggagagtg gcagcaacat gatactagtg
    13621 aggggcccag aaatcctctc aaagtgggta ggagaatcag aaaaggcaat acgggagatt
    13681 ttcagaaagg caaagacatc cgcaccgtgt gtggtgattt ttgatgagat ggactcgcta
    13741 gcaagaatga agtcaggcga agaaggcgga gtaggccaga caattctttc ccaaatacta
    13801 acagagatgg aggacagcgg atcctcacga gtagtggtaa tcggaataac aaacaggccg
    13861 gaccttttgg acaactcgct tctgcgacca ggaaggctgg agccagtcct ctatgtgccg
    13921 cagccagacg aaaaaggaag gcttgagata atcaaaatcc tcacagaaaa aatgccgctt
    13981 tccggcgatg tgaacctgca ggagatttcc gtttccactc aaaattatac cggcgcagac
    14041 ctagtagcct tgtgcaggga ggccggagta agcgcaatgc aaaataatgc cgcaaaaata
    14101 actagtgcca actttgcagc agctctcaaa aagattcgcc cctcaataac aaaggaggta
    14161 gaccagtggt atctggcaat aaaggaaact atttctaatg tcgtccccaa gacaatagac
    14221 aagacgtttt acggttaaaa attatggcag aatttccaat acgaaatcta gtgtacgaga
    14281 agataaagag cgcaggctca ctaacagacg aggagctaac caggagcctt tccaagtctg
    14341 gagtgacgct tgcgcaagac aagttcaaca aggttttgct cgacttggaa atactgggac
    14401 tagtcaaggt gtcatggctc acaaaggaca cgcgccgagt agaagcaact gttcaagaaa
    14461 aagaagtgga cgagtatgat gagcagaaca aggaaatggc tgaaaaggat tatgaggcat
    14521 catttccggg ccttgaagaa tcttagctat ttcacacgct gatcttgaat agatcttttg
    14581 cgtttttgta catcagcttg tccctgtaat ctttttttaa ctttagtttt gcaacaaagc
    14641 tgaggtacga gtccatattg gagattggcc agtcggttcc atacagcagg taacgcggct
    14701 cgcctgcata gttgatcagt tctgctacct tttcgaccat catgttttca aagaaatggt
    14761 cgaatttgcc aacagccaaa cccgacatgt ctgcatagac attaacgttc ttgtagacaa
    14821 cttcctggca gtcctctatc catgggtttc caagatggca catgacgatt ttgagctcgg
    14881 ggttgtctac tgcgacctca tctatgttaa gcgggtgcga gaatcttagc tttccttttc
    14941 cagaaaacgt atcaccagtg tgaatcatga caggaatgcc aaactccacg cacgtatcgt
    15001 agactttttg gtatttttta tcatacggat aataatgctc atagccgcag taaagcttta
    15061 ggccctttac cagtccgtcc tttatccatt ttttgtactg ccttagatcc tcatcggtgt
    15121 gattgtctat gctgtatccc gcaactattc ctaaattgtc atgctttttt gtggcctcga
    15181 ttagctgctg cgtcgatggt ctttgctcat ttaccacata tgaggacaaa ataattgaat
    15241 aatccacgtt gtgactcacc atgattctga gtagcctcct taccctgtca tctagtgagg
    15301 aaatgtttgc tagggattcg tattggttta ggtgcgtatg gcagtcaatt atcaaactaa
    15361 ttcaccttgt aatgcggatt cttccactct atgaatgtgg catagttttg tgagcggctg
    15421 tctttgagat agtttggcaa aatcttgatg aatttaaatc cattttttag ctgaaagccg
    15481 agcaccggct catcgatgtc gccttcaatt actttattga catattcttc tgctgaaaac
    15541 ttttcggcaa actcgcaata attgaacagc ctgccgcctg caattattct ccgcaggttt
    15601 agtttgatgc agaggttctt tctttcatcg taaagtctcg tcgcaacttt tagcctgcga
    15661 taatctgggt gtgtcgacac atcagctcca taaaggctgt caccacttgg attgtgattt
    15721 ccaaatctag gcccaccgca aattcccatc catgtatgtt cttggtattc cggctctaga
    15781 ttcactatta agctacttac cgagccgaca atctgcccat tgcagtcagc gcagaattgg
    15841 ccctcaggaa aaacctccag gtggcgttgc aactgttctg gcttccaaat catcccctcc
    15901 agcgccatga atggaaagga cacgttttgt aattcgacta tctttggaat gtcttcatga
    15961 gtggtgtgtc gaatttttat ttttgtttta tcattcattt acagatacta tgaagatctg
    16021 atcgaataca acatataaat gcctaaaaag aattttggct caaaaattag aaaaatattc
    16081 gctaattctg ggtttcttcg gtttcttcag attcttctac gggttcttct gattcctcaa
    16141 tagtttcctc agattcttca gtgacttcag attcttcact agtttcttca gcttcttcga
    16201 tggactccgc agctatttct tctgattctt cagattccgt catctgctca cgctcttcct
    16261 gcatttgttc cctcattttt tcgcggtctt cttgcatctt ttcacgttca tcttgcattt
    16321 gctctcttag cttctcgcgg tcttcttgca tcttttcacg ttcatcttgc atttgctctc
    16381 ttagcttctc acgttcttct tgcatctgct ctctcatttt ttcttgctct tctctcattt
    16441 gttctcgtat ggactctaat tcttctctgt cctgtccttc ttctctaatc ttttgaatct
    16501 cctctctcat catttcccta tcttctctca tttgatcacg gagatcagat acttctgctc
    16561 ggaactcttc ttgcatttgc attcgaatct catgggctct gtctctcata tcctctcgtt
    16621 gcgcccgcat gttaatcgca tcaactgcag acatgacatc tgcatccgac tggccttgag
    16681 catctctgac tagtactctg acgtggtccc taaattcacg gaagacatct tgatatgtag
    16741 ctcgaagttg gcgatatgat tctctgactt catctaggtc ctctctgcac tgcatcctaa
    16801 tgtctgctcg ttcttctggt gcggcgtctc gaactgcatc cctgcatgca tcaattattg
    16861 ctcttgtctc ttctctttgt tccctaaaca ggtcccgagt attatgtaca aagtcggcta
    16921 cttcccgtcc cagattgtca gtttcggttt cctcttcgat ttcgtcttca gcatcatcca
    16981 tgatctcatc ttcagtctct tcttgggttt ggtcttcgac atcatctact gtttcatctt
    17041 caatgtcatt ttcagagtca tctgtttggg caaaagcaaa atcaacgtac gtgtttgtcg
    17101 gaacaaaccc aatcatttga aaaccatttg ttgccggtgt aatcacgcct gcaagtaaaa
    17161 ctgacattaa aaataacgag gctgccccaa acttccgcac aataacgaac ttacctaaac
    17221 gcatttaaga cttactcata attgccatgt ctattcaaaa cagcatcttg aattgttttt
    17281 tatgattcag ccaaactgca gaattgagca gtagatttct ggtcttcagt aatagttaat
    17341 ccagattttt gcatcatgta tttttctttg ccctttctgc ttctaggcag tgatcgtagc
    17401 tttctcttac agcatggaca gaacgcgcca tccgagttta tgaaaatctc gcaaagatta
    17461 cagcgtttct ggccagcagc atatctgcca atctcaaggg gctttcttgc cttatatcga
    17521 acgcatgttc ctatgcaata ccccgtcatt tcacagaccc cgaacttttt tactttcttt
    17581 aatgctagca aaaaggcatc cgtctgagca gtgcgttaat tcttgatttt tcatttcctt
    17641 tccgcaatat ttgcagtttt tcaccatttt caaaataaat catttttgta actagtgtaa
    17701 ttgtctttgt tgattgtcaa gtctgagaat cagaggcata ttaaaaaact ctgtttattt
    17761 gcggttctgt caagcgcgat agctacaaga ttatttgtga acccgtgaat aatttccctg
    17821 atggtacttg ttgattggca gtaatgtatt gcgtcagcgt tttatacata gagatcccaa
    17881 gcacctaatg aaagtattag gcgaccgcaa ggattttgat cttgtgtttt ctcgtttgaa
    17941 taaaaacagt gccttgttca aggatgtcaa ggacattatt gataatctaa aaaatgatgt
    18001 catcgtaggt gagaggatca agtttgaaca gattccactg tactacaaaa aaagacatgg
    18061 tgttgacaat gcctttcatg tgtacctgcc agaggggatg aggttaatct attccattac
    18121 aaattatcag ggccaaaaaa ctgcttttct tatggagctg accggccaca agaactatga
    18181 gcgccgtttc aaatacaggt aatcacgtac atattcatat gtacaaatgc gtatatacta
    18241 gtatgtacat agtgtgagta aatgccagca cgcgttttta ataaatcgaa gaaaaatgcg
    18301 cctcagaaca acacatctgg aaacattgcc atcatgaact acattctaga aggcactcgc
    18361 aagtttaatc ccacccttag aagcctgaca atagttgaaa aactgatttg cgagaacaag
    18421 gaatttgatt ctaaatcacg cctctacaaa aaactgccta ccgggatgca gtatcctgtc
    18481 tttaacttta ttttaaaaat tttagaaaga caaggcaaaa tcatgcttgg tagtgacgga
    18541 gccatcttct ggacaggccc tgccagccca aaactacagc gaagcctgaa aaaagccgta
    18601 gaatactaaa tgagaattgt ctttcggcgt ttttttaggc ttaatctaac ctagaaccta
    18661 aagtgaaagg ccgcatctag cgcaattgct acgaagatta tagtcaagta cggagccgta
    18721 accttgtatg ccttccacgc aaagtccgat gtaggtgttt ttgtaagctt gtagtggtat
    18781 gcaagcatca ggcccccaga cgcagctgca actgccacat atacgattcc aagcccaaaa
    18841 gcatacagcg caagtgagta cggcaaaagt attgccgtgt ttgccagaat gtacttggat
    18901 gttttttgca tcccaatcaa tactggcagc ataggaacgt gtgcctctgc ataatcttcg
    18961 cgtatcttca ttgcaaggca ccaaaagtgc gaaggcgtcc acacaaatac caaaaacccc
    19021 actaaaaatc caagcaaatc aattcctact ggcggcacaa atccggttgc tgcagtccag
    19081 cccgccatcg atgccgcact tcctgcaaac cccccaatta ctatattgga cgagtttttt
    19141 cgcttaagcc acacagtata gactatgacg taaaaaaata ttccaagtgc gataaaaaaa
    19201 gtactggtaa gatttagaaa aattgcgcca tacaaaacgg atatcacgct tactgccagc
    19261 ccatacgcca aaacagagga cgcctttatc ctcccagacg gaatgggcct tgtgctggtt
    19321 cttttcatta atggatcaat gtcgcgatca taataatgat tcagtgcact ggatccggca
    19381 gacgcaagcg ctcctgcaat tatgatgtga acaagaccca tcggatcgag tgcggggccg
    19441 acaagctttg atgcggcata catcgaagtc acagcagtta ttacaagtaa aactatgatt
    19501 cttggttttg atacctcaag tatctctttt gccgccaaac actcaaaggc gcccccctag
    19561 tcatttaatt tcttcgaagt ttctcagacc ccaaaaggaa taagtatctc cgcctaggca
    19621 tcaagcacaa atgagcaatt gggacctcat gatgccagga atgggcctta ctgccatcgg
    19681 cctgaccgga gttactatag catacgccgg aattgcgcac acgttcatcg acggaatgca
    19741 cgcattgaca ggtcttacca tgttcattgg attgatcttt ttgtctgctg gaatcttgga
    19801 aggcggtgta tctactagca atcgcgcaaa agctacgact ttggtggttt tgggaatctc
    19861 gttttcattt ggtctggcgg cactcacgtt taacgcaatt actactatcc ctacatttgc
    19921 aggagttatg ctaatcatcg cagttccggc aataatgatg gcctatgtcg ccatgaagat
    19981 gccacagtat ttcaagccaa tatcaatcat atttgtacta gctactggtg ctgcagtcat
    20041 atcgtatgtg ggttttggcc tctatggccc atcccagtat ctagtcccgc caccagtaga
    20101 agaagaacct gaagtcccgg ttcccactgc gccgatattt gcaatttcca ttttgcctgg
    20161 gtcttcaatt tctggcaatc cggactattc cccagatgta gcacaggtgc cgcacggaaa
    20221 catcatagag tggaccaaca acgatgatgt tgtacatacg gttacaagca tagtcgatgc
    20281 agactataca tttgattcag tagtgataga ccccggcgag tcgtaccaaa ttgacacatc
    20341 gacgctatcc ccgggaacgt acgaatatta ctgcatggta cacccctgga tgactgcctc
    20401 ctttgtacta ggtgagggcg cacctgctgc tccggaattt gcaatttcaa ttcttgccga
    20461 ttcttcaatt cagggcaatc ccgattattc ccccgacttt gcacaggtgc cacttggaga
    20521 tgtcatagtg tggaccaatg acgataacac catacatacg gccactagca ctgcagaccc
    20581 gtcactcttt gattctgatt ttcttagtgc aggtgattca tacaggcttg atactacaac
    20641 actgggtccg ggaacgtacg
//
LOCUS       DASWKH010000060         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2018713, whole genome shotgun sequence.
ACCESSION   DASWKH010000060 DASWKH010000000
VERSION     DASWKH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018713"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /gene="sufB"
                     /locus_tag="VGA92_04590"
     CDS             <1..1340
                     /gene="sufB"
                     /locus_tag="VGA92_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9845723.1"
                     /translation="YVHLGKKGLSSDVVREISKLKDEPQWMLDFRLRSYEVFMKKPMP
                     NWGADLSAIDFQNIYYYAKASEKTEKNWDDVPADVKRTFDKLGIPEAEKKFLAGVGAQ
                     YESEVVYHNLREDLAKKGVLFLDTDTALKEQPEIFKKYFGKIIPPEDNKFAALNSAVW
                     SGGSFIYIPPGVKVDMPLQAYFRINAENIGQFERTLIIADEGSEVHYIEGCTAPVYSS
                     ESLHSAVVELVAHKDAKLRYTTIQNWSSDVYNLVTKRAYAYQGAKVEWIDGNIGSKIT
                     MKYPGIYLLEPKAHGETLSIAFAGKNQHQDTGAKMVHLASDTTSRITSKSVSRLNGRS
                     TYRGLLHVAKGAKNVKASVRCDALLLDDTSKTDTYPYMEINQEDATVTHEATVGKIGD
                     EQIFYLMTRGFSEEEALTLIVNGFMEPFTKELPMEYAVELNRLIKLEMDDSVG"
     gene            1366..2766
                     /locus_tag="VGA92_04595"
     CDS             1366..2766
                     /locus_tag="VGA92_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX9845724.1"
                     /translation="MQTLALSTITTTHVDEISSSKNEPEWLKQYRKGAFSVYQNLPEE
                     VSPLYNKYTDAKRMDPSLVSLSLESQSAVFDSLKPRLAELEKETHIIQTGSNIFRINV
                     PDELKSKGLVVSSIYDAIKTDGELVKKIFDSTDPKEDKYTALNSAAFNSGIFIKIPKN
                     LVLEKPIHIVSSISADGTSTISRNIIVAEQSSKASIVQELYAPEGAKQQAYLELLTVN
                     LQPNSNLQMTTLQAMNQSAVNFSTRNSTLAEDAKINWYLGLFGSMLSRYKTNYYLDGT
                     GASADDAEVIFGNNEQSFDLATNLVHKKPSTEGKAVQRAVLKNKSKSLFKGMIRILEG
                     ASQSRSFLSGRSILLDKDAKSDAIPGLEIFTNDVKATHSASVAQVDEEQIFYLGTRCL
                     SRSDAERIIVEGFMEPMSKSMSYQVRAWIAYLIESKWEGRDLILKSDEHLRSIVEVEE
                     VRYKEADQLETHYKYR"
     gene            2773..3081
                     /locus_tag="VGA92_04600"
     CDS             2773..3081
                     /locus_tag="VGA92_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX9845725.1"
                     /translation="MGNWVKACAKDQLGINEMFSFDHEDKRLLITNLGGKIYATDRIC
                     THAEADLSLGFVTEEGVRCPLHLSVFNLENGKPLNPPAEKPLKTYNIKIDQNAIYVEV
                     "
     gene            3085..4326
                     /locus_tag="VGA92_04605"
     CDS             3085..4326
                     /locus_tag="VGA92_04605"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9845726.1"
                     /translation="MQTSISVEQIRKDFPILKRTVRDNKPLVYLDNASTTQKPNQVID
                     AINDYYRNHNANIHRAVYALAEEATEAYEATRDKVAKLLNIKSREEIIFVRGTTEAIN
                     LVAYSWGRANIKEGDIIVTTEYEHHSNIVPWQLLTQEKNAKLEYIGVNDDGELILDQL
                     DKYLLTGKVKLVTFSLMSNVLGTITDVEKIVSKCRQASVPILIDGAQAVPHMKVDIQS
                     LDCDFFAFSAHKMLGPTGVGVLWVRKSILETMVPFHGGGDMIREVHKYETTWNDLPYK
                     FEAGTPNIADVVGFGAAIDYLEKIGMQTIREHEIELTKYALDKMSNVKGMTIYGTKDI
                     AKRGGVISFNFHDVHPHDVAHIVDREGVAIRSGHHCAQVLMERLNVAATNRTSFYIYN
                     TKEDVDALVKSLDKVAELFKL"
     gene            4323..4766
                     /locus_tag="VGA92_04610"
     CDS             4323..4766
                     /locus_tag="VGA92_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX9845727.1"
                     /translation="MSDPLYTEIIIDEAKYPQNKGTLEHPDISQHDANPMCGDSITLQ
                     MNIKDHTIDDIKWSGKGCTICTACTSVLTQLIKGKDIDDAKNMQKETILSELGLEHLI
                     QQSPIRIKCALLSLKVLKLGLYSYLASKLENNNFDSLKEEAASLY"
     gene            4768..5100
                     /locus_tag="VGA92_04615"
     CDS             4768..5100
                     /locus_tag="VGA92_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845728.1"
                     /translation="MSDSQTIPSQIRKIIFEKFNNGDVRFTNDEIFAFLQQNEMIDKS
                     LTIDDVEQHFTELCDSGVMRNIAQNFTTQWFKLFEPLEEFKCASCQTQSYINKTDERS
                     CPNCKASI"
     gene            complement(5093..>5530)
                     /gene="pgk"
                     /locus_tag="VGA92_04620"
     CDS             complement(5093..>5530)
                     /gene="pgk"
                     /locus_tag="VGA92_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9845729.1"
                     /translation="LLGDYPDVFFTPVDVAIDNAGQRVELDVRQLNKGDQIYDLGHKT
                     VEHYSKLIASAGTVFISGPPGYFEKEDFSYGTKAMLEAVSNSMATTIVSGGHLTSALK
                     KYGLAEKITHISTAGGALVLYLTGEKLPMIQSLEEAAKRYKPK"
BASE COUNT         1813 a         1215 c         1235 g         1267 t
ORIGIN      
        1 tgtacgttca tcttggcaaa aaaggcctct caagtgatgt tgtaagagaa attagcaagc
       61 tcaaagatga accacaatgg atgcttgatt tcagattaag atcttacgaa gtttttatga
      121 aaaagccaat gccaaactgg ggcgctgacc tctcggcaat cgactttcaa aatatctatt
      181 attatgcaaa ggcctcagaa aaaaccgaga aaaactggga cgatgtacca gcagatgtaa
      241 agagaacctt tgacaaactt gggattccag aggcagaaaa aaaattccta gcaggagtgg
      301 gggcacaata cgaatccgaa gttgtgtacc acaacctccg cgaggaccta gccaaaaagg
      361 gcgtgctttt cttagatact gatactgcac ttaaagaaca acccgagatt ttcaaaaaat
      421 actttggcaa aataattccg ccagaagaca acaagtttgc agcactaaac agcgctgtat
      481 ggagtggagg atcatttatc tacattccac ctggggtcaa agtcgacatg ccactacagg
      541 catactttag gatcaatgca gaaaacattg gccaatttga aagaacacta atcattgctg
      601 atgagggctc agaggtccac tacatcgaag ggtgcacagc gccagtttat tcatcagaat
      661 ccttacactc tgcagtagtt gagctggtgg cacacaagga cgccaagctc agatacacaa
      721 caatccaaaa ctggagcagc gacgtgtaca accttgtaac aaagagagca tacgcgtacc
      781 aaggtgcaaa agtagagtgg attgatggaa acattggaag caagataaca atgaagtatc
      841 ctggaatcta cctccttgag ccaaaggcac acggagagac actctcaatt gcatttgcag
      901 gaaaaaacca gcaccaggac accggcgcaa aaatggttca tcttgcatct gataccacat
      961 caagaatcac atcaaaatct gttagccgcc taaacggcag atctacatac aggggcctgc
     1021 ttcatgtggc aaagggcgca aagaatgtaa aggcatcagt aagatgcgat gcactgcttt
     1081 tagatgacac atcaaagact gacacatatc catacatgga gataaaccaa gaagacgcaa
     1141 cagtaacaca cgaggcgacc gttggaaaaa tcggtgacga gcaaatcttt tacctgatga
     1201 ctagaggatt ttcagaagaa gaagcactga cactaattgt aaacggattc atggagccat
     1261 tcacaaaaga gcttccaatg gaatatgcag tagaactaaa caggctcata aaactagaaa
     1321 tggacgattc agtaggataa actcctattt tccttttgtt gtaatatgca aactcttgcc
     1381 ttatccacaa ttaccacgac gcatgtggac gaaatctcgt catcaaaaaa cgagccagag
     1441 tggctaaagc agtaccgcaa aggcgccttt tctgtatacc agaaccttcc agaggaggta
     1501 tcgccactat acaacaaata cacagacgca aaaagaatgg acccatcact ggtctcactc
     1561 tcactagagt cgcaaagtgc agtttttgat tctctaaaac caagactagc agaactagaa
     1621 aaagaaaccc acatcatcca gaccggctct aacatatttc ggataaacgt tccagacgag
     1681 ctaaaatcaa aagggctagt ggtgtcgtca atttatgatg caataaaaac agacggcgag
     1741 ctggtaaaaa aaatatttga ctcaaccgat ccaaaagagg acaagtacac cgcactaaac
     1801 agcgcggcat tcaactctgg aattttcatc aagattccaa aaaatctggt tttggaaaaa
     1861 ccgattcata tcgtttcttc aatttctgct gatggcacct cgacaatttc acgcaatatt
     1921 atagtggcag agcaaagcag caaggcaagc atcgtgcaag aactgtacgc gccagagggg
     1981 gcaaaacagc aagcctactt ggaactcctc acagtcaatt tgcagccaaa cagcaatctg
     2041 cagatgacta cacttcaggc aatgaaccaa tctgctgtca acttttctac gagaaactcg
     2101 actctagcag aggacgcaaa gataaactgg tacctcggtc tttttggctc aatgctttca
     2161 cgatacaaga caaactatta tcttgacgga acgggtgcat cagctgacga tgccgaagta
     2221 atttttggaa ataatgagca gtcatttgac ttggctacaa acctagttca taagaaacca
     2281 tcaactgaag gaaaagcggt gcagagggcg gttctcaaaa acaagtcaaa atcgttattc
     2341 aagggaatga ttagaatttt ggagggtgca tcacagtcac gatcctttct ttcggggcgc
     2401 tccattctgc tagacaagga cgcaaaatca gacgcaattc caggacttga aatctttaca
     2461 aacgatgtca aggcgaccca ttctgcatcc gttgcccaag tggacgaaga acagattttt
     2521 tatcttggca cccgctgcct tagccgctca gacgcagaaa gaataatcgt tgaaggattc
     2581 atggagccga tgtcaaagtc catgtcgtat caggtaaggg catggattgc atatcttata
     2641 gagtccaagt gggagggaag ggacctgatt ctcaaatccg acgagcatct aaggtcaatt
     2701 gtcgaagtag aagaggtgcg ctacaaagag gccgaccagc tagagaccca ctacaagtac
     2761 aggtgaaaga cgttgggcaa ttgggtaaag gcatgtgcaa aggaccagct tggaattaat
     2821 gaaatgttca gctttgatca tgaggacaag cgtttgctaa taacaaatct tggcggaaaa
     2881 atttacgcaa ctgacagaat ctgcacacat gcggaagcag acctctcatt gggctttgta
     2941 accgaggagg gtgttagatg cccgttacat ctttctgttt tcaacttgga gaatggcaag
     3001 ccgctaaacc cgccggcaga aaaaccacta aaaacgtaca atattaaaat agatcaaaat
     3061 gcgatctatg tggaggtttg agaattgcag acatctattt cagttgaaca gattcgcaag
     3121 gactttccaa ttctaaaacg aactgtacgg gacaacaagc cgcttgttta tcttgataat
     3181 gcatccacaa cacaaaagcc aaaccaggta atagatgcca taaatgatta ttacagaaac
     3241 cacaacgcca acattcatcg ggcagtctac gccctagctg aagaggcaac tgaggcatat
     3301 gaggccacac gggacaaggt tgcaaaactc ctcaacatta agagccgtga agaaataatc
     3361 tttgtgaggg gaacaacaga ggcaataaac ctcgtggcgt actcgtgggg gagagccaac
     3421 ataaaggaag gagacatcat tgtcacaaca gaatacgagc accacagcaa cattgtcccc
     3481 tggcagctcc taacccagga aaaaaatgcc aagctagaat acatcggcgt taatgatgac
     3541 ggggaattaa ttcttgatca gctagacaag tatctcttaa caggcaaagt aaagctagta
     3601 acattcagcc taatgtcaaa cgtgcttgga acaatcaccg atgtggaaaa gattgtttca
     3661 aaatgcaggc aagcctcagt accaatacta atcgacggtg cacaggctgt gccccacatg
     3721 aaagtggaca ttcaaagtct tgactgcgac ttttttgcgt tttctgctca caagatgctt
     3781 ggccctacgg gagttggcgt cctttgggta agaaagagca ttcttgagac catggtgcca
     3841 ttccacggcg gcggcgacat gataagagaa gtccacaagt atgaaactac atggaatgat
     3901 ctgccgtaca agtttgaggc aggaactcca aacatcgcag acgttgtggg ctttggggcc
     3961 gccatagact accttgaaaa gattggaatg cagacaattc gggagcacga aatagaactt
     4021 acaaaatatg cgctagataa aatgtcaaat gtaaagggaa tgacaatcta cggaacaaaa
     4081 gacattgcaa agcgcggcgg ggtaatttcc tttaactttc atgacgtcca tccgcacgat
     4141 gtcgcacaca tagtagacag ggagggggtg gcaataaggt ccggacatca ttgcgcccaa
     4201 gtactaatgg agaggctaaa cgttgcggca acaaaccgaa ccagctttta catctacaac
     4261 acaaaagaag acgtagatgc ccttgtaaaa tccctcgata aagtagccga gttgttcaag
     4321 ctatgagtga tccgctctac acagagataa taatcgatga ggcaaaatat cctcaaaaca
     4381 agggtacgct ggagcatcct gacatatcac agcacgacgc aaacccaatg tgcggcgaca
     4441 gcatcactct tcagatgaat atcaaggatc acaccattga tgatatcaag tggagcggaa
     4501 agggatgcac gatttgcact gcgtgcactt cggttctgac gcaactcata aagggaaagg
     4561 acattgatga tgcaaaaaac atgcaaaaag aaacaatact gtctgagctt ggactagagc
     4621 acctaattca gcagagccca attaggatca agtgcgcgct cttgtccctg aaagttttga
     4681 agcttggact gtattcttat ctggcaagca aattggaaaa caataatttc gatagcttaa
     4741 aagaagaggc ggcaagcctc tattaatatg agtgattctc aaacaattcc atcacagatt
     4801 cgaaaaataa tctttgaaaa attcaacaac ggggacgttc gattcacaaa cgacgaaatt
     4861 tttgcgtttt tgcagcaaaa tgaaatgata gacaaatccc ttacaattga tgatgtggag
     4921 cagcacttta cagaactgtg tgattctggg gtgatgcgga acattgccca gaactttact
     4981 acgcaatggt tcaagctctt cgagccactt gaggagttca agtgtgcatc atgccaaacc
     5041 cagagctaca ttaacaaaac agatgagcga agctgcccta actgcaaggc atctatttag
     5101 gcttgtatct tttggcagcc tcttctagag actgaatcat tgggagcttt tctccagtca
     5161 gatacaaaac aagcgctcca ccagcggtgc tgatgtgggt gatcttttct gccagaccgt
     5221 atttttttag tgctgatgtg aggtgtcccc cgcttactat ggtagttgcc atcgagtttg
     5281 atactgcctc aagcattgcc tttgtgccgt agctaaaatc ctccttctca aaatatccgg
     5341 gcggccctga gatgaaaacg gttcccgcgc ttgctatgag ctttgagtaa tgttcgactg
     5401 ttttgtgccc aagatcgtat atctggtcgc ccttgttgag ctgcctgacg tcaagctcga
     5461 ctctttgtcc tgcgttatct attgcaacat caactggggt aaaaaagaca tcaggataat
     5521 cgcctaagag
//
LOCUS       DASWKH010000061        29072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_146228, whole genome shotgun sequence.
ACCESSION   DASWKH010000061 DASWKH010000000
VERSION     DASWKH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 29072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29072
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146228"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /locus_tag="VGA92_04625"
     CDS             <1..1968
                     /locus_tag="VGA92_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HEX9845730.1"
                     /translation="TLLFEKQGKEYVINMIDTPGHIDFTGRVTRSLRAIDGAVVVSDS
                     VEGIMTQTETVTRQALEERVRPVLYINKIDRLIKELRLTPDKMQEWLANIITTFNGLI
                     DTYAEPEYRDKWKVSIQDGSVSFGSAKDRWGFNIEVMKEKGISFKDIYAAYAEGGSVD
                     ELAKKAPLAEAILGMVVKHHPPPHVAQKYRIPKIWKGDLESDIGKAILNCDDTGPAVM
                     MIVNMAMDPAAGPVAIGRLFSGTLKDGQSVFLIDTKREGKIQSVNFFMGNQREMVGEL
                     GAGNIPALLGLTEARAGQTLASVKDVSLFEGIQYVSEPVVQIAVEAKHPKDLPKLVEA
                     LRRLTIEDPNLVVKINEESGETVIAGMGVLHLEIATALIADAGVQIVTSQPLINYRET
                     IQGGSDVVMSKSPNRHNKIFMKVEPLEPEIADMIRSGQLSEMKDKKEIATILRGKGWD
                     ADIAKSVMKFDPRGNVFLNGTKGVQFIQESSDSILSGFDEVMKEGPLAKEQVRNCKFT
                     FTHFVPHEDSAHRGLSQLSPASRRACLGAMLKAQPILLEPMLGIEVRVPQDLIGDVAS
                     VLSGKRGKVLDMQQKGITSIVTGELPASETFDLSETMRGQTAGKAMWNTHFKAWTAIP
                     KSIQTTLIADIRKRKGLSPDPPTADEFLDSE"
     gene            2268..5513
                     /locus_tag="VGA92_04630"
     CDS             2268..5513
                     /locus_tag="VGA92_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX9845731.1"
                     /translation="MKRLTLLLLSSILILISVNDQANAASVPDPPTNLTATAVSPTQI
                     NLFWSAPSYNGDSAITGYKIEYKIGSGAYTTLVANTANTSTTYSHVGLTTDQSYSYRI
                     SAINAIGTSTSSTEASATPTSSSSGTLPGSPTNLSAIPVSPTQINLFWSAPSNNGGYP
                     ITGYKIEYRVGSGSYSTLVSDTGSTSTTFSHTGLTTNQAYLYRVYTITSFGTSEDPSP
                     EAFAQPKSTSTLNTPSAPTNLTATAVSPTQISLSWTVPSNNGGAVITGYKIEVKSGSG
                     SYTTLVSNTQSTITTYSHTGLTTGTTYTYKVSAINSAGAGTASNESSATPTSSSSSGV
                     PGAPTNLSAMAVSGTQINLSWNAPSNNGGYPITGYKIEYRIGSGSYTTLVSNTQSTTT
                     TYSHTGLTSGQLYVYRVSAINSAGTGSPSSESSATPTSSSSASTPGTPTGFTATTASP
                     TQINLSWNAPSSNGGSAITGYKIEVNSGSGSYTTLVSNTQSTTTSFSHTGLTTGTTYY
                     YRVSAINAIGTGASTEASATPQETTTPTLTAMAVSPTSITLSWAPPSQTYKQAITGYK
                     IQEKIATDTFVTIEERTISTATTYSITGLTTGNTYTYVVSAHFSLGASPNSNEASATP
                     TTTSAPPPSQPTTNPPSAPTNLSTTPTSPTQINLSWNAPSNSGGSPITGYKIEVRSGT
                     GSYTTLTSTQNSTTKYSHTGLTTGTTYSYRVSAINSVGVGSPSSESSATPTTTSVSPP
                     SQTATPSPPTLAATLISPTQINLSWTANTGDSAITSYKIEYKIGSDQYRTLTSKATTI
                     TYSHAGLLANTYSYRVYAINSAGTSSPSNEVSIKTSEQTLPPLEPEETMCGPGTVLNE
                     QGVCVLQESEPEPEQSEPKETHIPGFPDPDKDPQYYINRYNTESTYKEWFDRNFPDQT
                     IYEVVGVPEPEPQEKKCGTGTHLENGMCVLDETQNQFPLSGCLIATATYGTELAPQVQ
                     QLREIREHTLLSRSSGTAFLGMFNYVYYSFSPTIADWERQNPAFKEIIKVSITPMLST
                     LSLLNNAKIDSEQSLLGYGLGVILLNVGMYFGIPALLILFIRNKTKTKCKP"
     gene            5561..5977
                     /locus_tag="VGA92_04635"
     CDS             5561..5977
                     /locus_tag="VGA92_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845732.1"
                     /translation="MKVVGLILGIFLVWPLLTGSFFAEAKIDEPMAITSMKKDIKEIK
                     KPSQDKSVKITSITKFRMLAILYVVGVEVCAGKDKLYSPELNLISDRDSITVKVAGLI
                     MPKACKTAEFFIRANNPNSITVNFANTDYHYPKRSP"
     gene            6442..7377
                     /locus_tag="VGA92_04640"
     CDS             6442..7377
                     /locus_tag="VGA92_04640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9845733.1"
                     /translation="MGFKVATNFVVFASVLVLLSLGSFASYAEAQTFEMYVQKMPPHW
                     EKQFGDVLTKATQYWEERIPGLKFGVVKFVDQADFVVEWASQYGEGKIGYYSTNTANE
                     YGKPKLAISLGFFKDKKWNLVSTDYALEITKHELGHAIGLPHSDDPNDMMYPIIESYE
                     SWQQSHAEKPKATSKTPEQNLQASTKSTIDWQNKSQKYQKLASDKIFNLETKIDTAKS
                     LLDSTNYENMAAKVEIEKAWTAFWWAKKYYANAEKMQTDGGAFLLQSEYQDSYKKFKS
                     SYDYAKKLEKKLADVTKYHKKAYGLEYGKNIVIES"
     gene            7386..7544
                     /locus_tag="VGA92_04645"
     CDS             7386..7544
                     /locus_tag="VGA92_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845734.1"
                     /translation="MKKAELRKMVEEYGILKSKSDSDDQVNPKILKKIKEIERRYFHE
                     TGQDLSLS"
     gene            7593..8117
                     /locus_tag="VGA92_04650"
     CDS             7593..8117
                     /locus_tag="VGA92_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845735.1"
                     /translation="MLTRKSISVIIGGSVAAAILLVVVASGQLGMQEIASTQLIEEQN
                     VSVIEKQTQEQMQVYRINTECELIYGLASGSYPNGEKLPALKILDLVDKYPEFGPWET
                     ILRDNQTRAEFFKQSLPEDFGDVLVNAVMSESSINPELDEIASLVADPQGRAKLQQAF
                     QEFNCKAYFDERAN"
     gene            8171..8950
                     /locus_tag="VGA92_04655"
     CDS             8171..8950
                     /locus_tag="VGA92_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845736.1"
                     /translation="MRSLHILIISIIAIVVSYYVISAFVLQQQAINKQSVYVHLQPEW
                     DSYPGNIVYDITNVWSQTENRQLNSKERLEMSKSTNVDELRYVHGKSYILVQHDNTNC
                     HDSWEPHYARFGADVIRHQIEYLTGLQSNPDPNVTLYNPIPSKQDTTEHESQIKLGYS
                     QFIPICTEKDTTSFDYSVRINDNSVGFDVYFVPSIQEQENYDQNNNKFQYYETGQCSG
                     KNYASFSGTCYDVRKDSGLLIVIPDNLSLPLTKLDVWLYEK"
     gene            9041..9502
                     /gene="msrA"
                     /locus_tag="VGA92_04660"
     CDS             9041..9502
                     /gene="msrA"
                     /locus_tag="VGA92_04660"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9845737.1"
                     /translation="MKATFGAGCFWCVEDVFRRTKGVTSVMVGYSGGSTKNPTYNDVC
                     TDLTGHAEVVQVDYDPNVLPYEELLDVFWNSHDPTQLNRQGPDIGTQYRSVIFYHDSQ
                     QEAIARKSKEILDVSGKFGKKIVTEIQPAAEFYKAEEYHQQYYAKCGMAKY"
     gene            9913..10305
                     /locus_tag="VGA92_04665"
     CDS             9913..10305
                     /locus_tag="VGA92_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845738.1"
                     /translation="MCLRYAFLLLFVVMLQNVYAQDLPVKLADSTKKTVEVGKQIQIA
                     ADLKNNQDVKQDFAYIVQIQNEDGVIVSLAWITGTLAPAQSFTPALSWIPNEEGRYEA
                     TIFVWESINNPSALSPTLSLGIDVNQFA"
     gene            complement(10505..11110)
                     /locus_tag="VGA92_04670"
     CDS             complement(10505..11110)
                     /locus_tag="VGA92_04670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX9845739.1"
                     /translation="MKIGPILTELERQSHLEKSRKVDVKPEDRMLAITKDTGGFFHAL
                     LVAIKAKKALELGTSTGYSTLYIADALLSCHKNPVIMTVEKNPSKIIRAKRNFERAGV
                     SDYIKIIPSHILDVLKNMPKKPTFDFALIDADKENAKKYFDLVLPLLRVGGIIATDNM
                     LYPEKYREIMRNYSKYIVKKKNVWTVTLPIGNGQEISIKTK"
     gene            11235..11615
                     /locus_tag="VGA92_04675"
     CDS             11235..11615
                     /locus_tag="VGA92_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX9845740.1"
                     /translation="MNAYKNASLIEIVEDIVDIDTGMRFAAIIDLKGNILESIMKKGK
                     TSLKTQKEEEHFCKQIAQRRKMRKEFDKSLGKVRYVQVEREKVIQFVVYPKRKTIYFT
                     MEPETSVNKKMAIINRVKKLTMHL"
     gene            11809..12084
                     /locus_tag="VGA92_04680"
     CDS             11809..12084
                     /locus_tag="VGA92_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX9845741.1"
                     /translation="MKILVDEMDDGWDEKLRTLGYEAYSVKKLLTEGKKLHTDYSVIN
                     YAKENEMILITRDTESGQACTENNLPCILLDNNEIFKIVLEKLKGFV"
     gene            12122..12376
                     /locus_tag="VGA92_04685"
     CDS             12122..12376
                     /locus_tag="VGA92_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845742.1"
                     /translation="MESCDRRTRAYKNGKTFDQCVQIAEALNPEFKKTIETQGKVLWT
                     EILSKVDHDELVYKLTLKYLRRDGYDIGNWLIPEVKKFSS"
     gene            complement(12373..13179)
                     /locus_tag="VGA92_04690"
     CDS             complement(12373..13179)
                     /locus_tag="VGA92_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845743.1"
                     /translation="MAESRDGRLLIAIGFVSVAILLLIYARLGNSEITPAAISSIERL
                     GVAFYFMLILALGAIAYGFFQYHHQKVQAGKDDVFSIIAQVTWNKKSRRIFVATSIGY
                     GIFFALTSGILVYQPDVIFSKHYGVVVPSAQIAPCCGDVGYVPKLIFYMTEHVGLQII
                     PINLILQITVSYLVGLNAAIAVNAFSILKKGTGIGSIAATTGLFIACPTCAGTFLSLF
                     VSASGTIAFTLAITQIQTILIAVSIPLLAFTPVIMAKKIKSSGWSCKVES"
     gene            13294..13767
                     /locus_tag="VGA92_04695"
     CDS             13294..13767
                     /locus_tag="VGA92_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845744.1"
                     /translation="MKSGTKTKKRTIATAEIPISVCDVMKDNTSKIIKKLESQIPFST
                     QHYFDLYAAFLHSIDDVFGTCYIAEKQFFDKIGFDQNMLKSIKYVSDQFTNVLLTQVE
                     MTSNLQKSHLQFQKSVLGMSEQYTHSFVDSYWKSLTELNRFLENTKKTYKLEPQN"
     gene            13820..15151
                     /locus_tag="VGA92_04700"
     CDS             13820..15151
                     /locus_tag="VGA92_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9845745.1"
                     /translation="MTKEQVQNSVAKAKAAFGSWKNTLEKRKSCLYNLTDYLRKNKIK
                     LAEAATREMGKVIKESISEVEKCAWALEFYADHGPNFLSDEILNTDARKSFITFEPLG
                     VIGSIMPWNFPYWQALRFTAPSLIAGNTIVMKPSSVTIQCGLEMEKACREAGFPDGVF
                     QILVGSSGVATHLIESNVNAVTFTGSTKVGALVGQKAASLLKKCVLELGGSDPFIVLE
                     DANVEKAANGAVKGRFINCGQSCIASKRFLISKKVANEFIEKFVHNASRLQMGDPMVI
                     ENDIGPLVNKEALENISAIVDDAKKKGAQVLLGGERMGDKGYYYKPTILTGITADMRI
                     SKEETFGPVAPITVFEDENEAIRLANDSEYGLGASIWTEDLNKAEMLSKRVESGIVTV
                     NNVVISDPRIPFGGIKNSGYGRELSKYGMLEFVNIKSVRFYDQLIHHHYVE"
     gene            complement(15469..16707)
                     /locus_tag="VGA92_04705"
     CDS             complement(15469..16707)
                     /locus_tag="VGA92_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9845746.1"
                     /translation="MKASTPQLSLDRIRTVFRQKTIQQLLQKTFKFRLYPNHTQIKQL
                     HHTLGTCRWVYNTMVEKIHSEGFQSRNDLNYFLTELKESESWLYSHHSKMLQTISAQV
                     DGAQKALVQLRKNGHKTGSLKFARFLEYRTFTYNQSGFEIKDDFLHLSKIGKIKIVEH
                     RTIPENHIIKQITVSKSKSGRWHACITVDIDAVIPKISLRKSVGIDVGIKNFVYDSDG
                     FVTPNPLNYSKMLKPLRRIQRKISRRQKGSQNRKKATRFYQIIHERIKNKRKDFLHKI
                     STQYAKKYDVVFLERLQKLNMVKNHKLARNILDSGWGTFTNMLYYKTMVVEVPARNTT
                     INCSRCSNMVPKTLAVRTHKCDVCGLVLDRDHNASINILKNGLVDIFNIKLPQELREV
                     TPVEIISRSRKQEATSFSEW"
     gene            16774..17199
                     /locus_tag="VGA92_04710"
     CDS             16774..17199
                     /locus_tag="VGA92_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX9845747.1"
                     /translation="MNINQKEEPDYATSKIAYVGFIDPYALEGIIVLQSDDKKEFHMR
                     AFSGEVARHIKSFVEGKRDTLPTIYNLIEQLCEENELYLVKVKVYESGSVLRANLYFT
                     GKKDLVLRNYRASDAVALGSFYNIPILVRKSLLKERVEA"
     gene            complement(17196..18287)
                     /locus_tag="VGA92_04715"
     CDS             complement(17196..18287)
                     /locus_tag="VGA92_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--phosphoribosylaminoimidazolecarboxamide
                     ligase family protein"
                     /protein_id="HEX9845748.1"
                     /translation="MIRPSEIQKIANNYSDFRIGVLGSHSALEIMDGAKDEGIQTVVI
                     CQKGRDVPYRRFKRIADEIIMVNNFKDMASAKMQKLFRDTNTIIVPHRALTAYLGYDT
                     IENKFKVPIFGNRALFQAEERSFAKNQYYLLEKSKIRHPKLFKNPRKIDRPVIVKVQE
                     KARRLERAFFTASSYDDFLKKSQDRIKKGIIRKEDLKAASIEELVIGTYMNFNYFHTP
                     ISDQVDFIGIERRLQTNLHDYNAIPARQQLDIDIDIQNIEVGHTPASIRESLLEKVIA
                     MGDKFVAGVKKVYPPGIIGPFSLQSVITKDLDLVVYDVSLRVPGNPIVATTSPYTKYQ
                     YGQTFGVGRRIAMELKSAQEQGRLAEVLS"
     gene            18653..20149
                     /gene="pstS"
                     /locus_tag="VGA92_04720"
     CDS             18653..20149
                     /gene="pstS"
                     /locus_tag="VGA92_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX9845749.1"
                     /translation="MKILAATLMTVLIASVFSQVAIAQNQFTITGAGATFPFPLLDLW
                     RVKYNEAHPNINMNYQSIGSGGGVKQHVELTVNFAASDAPLTDGEMELAPDSLTIPEA
                     IGGITVTYNIPEIPQSGLKLTGQQVADIFQGKITKWNDAQIQQTNPDLPLPDESILVA
                     HRSDGSGTTYAFTEYLTMVSQDWDDQVGFGKSVPWPAGVGAAGNEGVATTVRTSPYSI
                     GYVELAYAFQNNMAVAFVQNADGTAFVEPTFDTLSAAAAGAAAGLPAAHEHWQEVSIN
                     NSPGPNSYPIVSFTYILLHQNLEAATPSKAHAQETVNLIKWMITEGQKYNPELLYVPI
                     PAEVTQLGLDGLDRVTYNGQPLFAEAAMEQPQDTQDAEGGGCLIATAAYGSEMASQVQ
                     LLREVRDNVLFSTTSGANFMTSFNSIYYSFSPTVADWERNNPAFKEVVKTAITPMLST
                     LSILNYVDIDSEQEMLGYGVGIILLNLGMYFVAPAIVIVKLRSKFNSK"
     gene            20309..20581
                     /locus_tag="VGA92_04725"
     CDS             20309..20581
                     /locus_tag="VGA92_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845750.1"
                     /translation="MYIRKDILLKLYEHGELNQSKLMSYCGLNNVKHKGILDDMVKKE
                     MIVRTEEPWGNKNIIKYKISDKGKGILDEVLGPYEELFPRGGKEDE"
     gene            20630..21025
                     /locus_tag="VGA92_04730"
     CDS             20630..21025
                     /locus_tag="VGA92_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845751.1"
                     /translation="MTDSEKNGSNIFKTMDEIIFQLNRTKKMFIIMILTVMIIPPITF
                     LIFTAIYDPPFESQEKTEHDGSKPKFTIFRVIPLIISAVWLGIGIRQWIVLSKWTKKY
                     DRYKELQEKIDKKLDDDEEKDDEKDSSSR"
     gene            21108..21860
                     /locus_tag="VGA92_04735"
     CDS             21108..21860
                     /locus_tag="VGA92_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845752.1"
                     /translation="MNQGKIRDFRTHVFSFTLLGIIVSFALVLFSPSQALAQEVQVTK
                     INSDFTVVAGEEIKKNPTAIQILQNIEIAKKRLAEMQNVEKQKTEREKFIDEQRRIAK
                     ELLEKDLNRMNKDYEDYTPKNAFARFVSGFNSTHHAIYWDQFEYMNQKIKIATQAKQT
                     VLANGGSYVEAQAEFIKYASMPRVEMISFVSDLNIKYGFTDAALQQYFDENGKLPRYE
                     NDDVAVCYACDKYEQVKVEMLAEHESSKNEST"
     gene            21985..22542
                     /locus_tag="VGA92_04740"
     CDS             21985..22542
                     /locus_tag="VGA92_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX9845753.1"
                     /translation="MRLVIGMTGSTGVVYGVRALEVLKECKIETHLVITEWAKKCLAM
                     ETDYKFDDVKSLATSYSDDSNLASGISSGTYKTDGMIVIPCSMKTLSSIANGYDETLV
                     ARAAGVTLKESRRLVLVPRETPLTAINLENMLKLARLGVVILPPVPGFYTRPKSIEQI
                     IDHTVGKCLDQFDIEHNLYKRWGGT"
     gene            22549..22980
                     /locus_tag="VGA92_04745"
     CDS             22549..22980
                     /locus_tag="VGA92_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9845754.1"
                     /translation="MLRFRLEKIIKAERQHVFDIVANYENFEKILPQYFPSIRIRSVR
                     DNTAIVEEHLRLGSRELVMMTKHVTKYPDVHEVFVVGGDAKGSHFVEKYEKIPEGTKM
                     TVDVNIKLKGIMVIVGALGKHKISSEFGKIIDEFAKIAEKS"
     gene            complement(22977..23171)
                     /locus_tag="VGA92_04750"
     CDS             complement(22977..23171)
                     /locus_tag="VGA92_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9845755.1"
                     /translation="MLGNIANFIAGQEWIFIIIIAGILIFGAKKIPELARTLGKSRME
                     YEKGKVEAEKELKDLKENKD"
     gene            complement(23193..23996)
                     /gene="tatC"
                     /locus_tag="VGA92_04755"
     CDS             complement(23193..23996)
                     /gene="tatC"
                     /locus_tag="VGA92_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9845756.1"
                     /translation="MSEFEGIYAHLAELRSRATRILIAAGIIVVFILTFRAEQIVLSG
                     ITLYYPTPDPLHNIAAQITNHMKATLVPADVQLIQTAPGQAFYAQVYIAALAGIVTSM
                     PIIVRELTSFIKPALSENEVNAVRSIALPAIALFVGGLIFSYLVVIPFMLDFLYQYGE
                     SAGLVTFLNVVDFVTFVLQFLLAFGVAFQLPLIMYAATVSGLTDVKFWRRNVRYAIVA
                     LVVIGAAVTPDGSGVTMWFIAGPMIGLYMTGMIILERRERKTQRLKSQS"
     gene            complement(24044..24511)
                     /locus_tag="VGA92_04760"
     CDS             complement(24044..24511)
                     /locus_tag="VGA92_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845757.1"
                     /translation="MTRHRYWKITSDEISKADYEQSKVLNWEIKGIKEPDDAAIFIGV
                     FLYRNGTPIDYVPTKGVVFYHNNVTKNEVGEVTKFLKDRFGGTEKEKGDRMFLVDSKE
                     IYSGKEIGLLAKDLETKFKLKAIISIEFQNLTEEERKNSGLADAKLLPIPGKN"
     gene            24605..24865
                     /gene="purS"
                     /locus_tag="VGA92_04765"
     CDS             24605..24865
                     /gene="purS"
                     /locus_tag="VGA92_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9845758.1"
                     /translation="MPLFEVQVTIENKPGMNDPEGQTILNDLVLKGSYSSVKKIRSAK
                     MLKFQVNAATKQAAQNTVKKLCDDLRIYNPIASKVTFTATSS"
     gene            complement(24862..25281)
                     /locus_tag="VGA92_04770"
     CDS             complement(24862..25281)
                     /locus_tag="VGA92_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9845759.1"
                     /translation="MGYVRDIMNKHVITISKDKTCLEAATLLHEKDISFLVVTEGQNP
                     VGVVSEADFVRKLVAANKIASQTKVSEIMSPKFRWVEPTTTIEDAVQKMLNNHIRRLV
                     VLENQNLAGVITQTDLAAHLRSKLLIDETVKSIGDQD"
     gene            25359..26039
                     /gene="purQ"
                     /locus_tag="VGA92_04775"
     CDS             25359..26039
                     /gene="purQ"
                     /locus_tag="VGA92_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9845760.1"
                     /translation="MKVGVIVFPGSNCDRDMFHVLQDVFKLDVKYFWHEDRLPKNLDA
                     VVLPGGFSYGDRLRAGVIAAHSPVISDVKRMADKGMPILGVCNGFQILVEAQLLPGVL
                     LRNTSLNFMCRWTELIVENNKTPFTNQFKLKQKIPIPIANGEGRYYVDKTTLAQLKKN
                     NQIVFRYGENINDSVFDIAGICNKEGNVVGMMPHPERAVEAEINPKDSKPSSLIFESL
                     ITTVGAKR"
     gene            26036..28201
                     /gene="purL"
                     /locus_tag="VGA92_04780"
     CDS             26036..28201
                     /gene="purL"
                     /locus_tag="VGA92_04780"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9845761.1"
                     /translation="MSLQPQELQYLSKKIGRKPNETETHIVAAEWSEHCSYKSSKKHL
                     KMLPTSGPRVIHEKGFDSGVLDVGDGYVVTVHIESHNHPSAVEPYGGAATGVGGVIRD
                     ILSAGTRPIAILNGLRFGDIEKDSHARWLFKNAVTGIADYGNCLGIPTVGGEVEFDKC
                     YTNYALVDVASIGFGKKEQLIKNHAQKDDIVVLIGGSTGRDGIGGSQFASDSLEIEDR
                     SAVQIPDPFIEKLIIEATLEARNEGCIKAIKDLGGGGLSCAISETADALGIGIELDVN
                     HVHTREPNMNPTEIMTSESQERMLIITDRQKLGKLEKICKKFHVSCAVIGKVTGNKLM
                     KVTTNKKVVASMPAEVVANAPLLDRPSKEPAYLKELQKRQEPKPAKDLSKTLLKLLSS
                     PNIASKIWVYGQYDHEVGIRTVVKPGSDAAVLRLDNGKYLAAKIDGNPKHCYLDPKNG
                     AIGCFEEACRNVVCVGATPIGMVDHLQFGNPEDPEIFWTFLESLKGITEFSKFTNVPC
                     VGGKVSLYNETPQGPIKPTPLIGVLGLIEKKPLKPQTLETGDLLLIVGKTKDELGGSE
                     YYEYIHDFIGGKCPVVDFEESNKNMKAVLHLIQNDKIKAAHDCSKGGLAIAICELAVT
                     SKIGCNITLENIPQEKSNVSKLLFSESHSRYLLVIKKSNIDAVVDYLKKNKIAFGLIG
                     KFEGSDINFSENNKPVAKLRVDKAHAKWFNSLRELVLHG"
     gene            28194..>29072
                     /locus_tag="VGA92_04785"
     CDS             28194..>29072
                     /locus_tag="VGA92_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX9845762.1"
                     /translation="MVKENCGVVGIFSRDGVNVIPMVIDALRALQHRGQEAWGIAIPQ
                     KPPLKRLGLVSAASSELQKITQEYAAPAAIGHVRYSTVGKSNLDNAQPLKVKDLCIAH
                     NGTIANVSELANLVGGCTFTPQNASDTLIAAQRLVSLMSTNGNLGSALSILKNEMAGS
                     YCFTFVSDDNAVYAARDPRGFRPMVLGSKEEGQVSVVASESSALTAVGAKLTRDVLPG
                     ELLKFSDKGVESEMFSEEKKHAHCSFEFTYFAHPTSKMEGTNIYVARKRIGQFLAKKF
                     PIKEADLVIPVPDSARP"
BASE COUNT         9268 a         5846 c         6014 g         7944 t
ORIGIN      
        1 actttacttt ttgaaaaaca aggaaaagaa tatgtcatta acatgattga tactccaggc
       61 cacatcgact ttactggacg tgtaacgcgt agtctccgcg caatcgacgg agcagtagta
      121 gtatctgatt ccgtagaggg aatcatgact cagactgaaa ccgtaactag gcaggcactt
      181 gaagaacggg taaggccagt tttatacatc aataaaatcg accgcctcat caaggagcta
      241 aggcttactc ctgataaaat gcaagaatgg ctcgcaaata tcattactac gtttaatggg
      301 ctaatcgata cctacgcaga acctgaatac cgtgacaagt ggaaggtctc aattcaggat
      361 ggaagtgtct cgtttggctc tgctaaggat aggtggggct ttaacattga agtgatgaag
      421 gaaaaaggaa tctcatttaa ggacatttat gctgcatatg cagaaggcgg gagcgttgat
      481 gaacttgcaa aaaaggcacc gcttgcagaa gcaattcttg gaatggtagt aaaacatcat
      541 ccgccaccac acgtagcaca aaaatataga attccgaaaa tatggaaagg tgatctggaa
      601 tcggatattg gcaaggcaat tttgaattgt gatgacaccg gtccggcagt aatgatgatt
      661 gtaaatatgg caatggatcc cgcagcagga cctgttgcaa ttggcaggtt attttctggg
      721 acattaaagg acggccagtc ggtttttcta attgatacaa aacgagaggg aaagatacag
      781 tctgtgaact ttttcatggg aaatcagcga gagatggttg gcgagcttgg ggctggaaac
      841 attccagcac ttttgggact gactgaggcg cgggctgggc agacacttgc atctgttaaa
      901 gacgtttcac tattcgaagg aatccagtat gtgtcagagc cagtagttca gattgcagtg
      961 gaggcaaaac atccaaagga cttgccaaag cttgtagaag ccctcagaag gttaactatt
     1021 gaggatccga atcttgtagt aaaaattaat gaagaaagtg gagagactgt cattgcagga
     1081 atgggtgtgt tgcaccttga gattgcaact gctttgattg cagatgccgg cgttcaaatt
     1141 gttacgtcac aaccactgat taactacagg gagacgattc agggcggctc tgacgtcgtt
     1201 atgtcaaaat caccaaacag gcacaataag atattcatga aggtggagcc actagagcca
     1261 gaaattgcag acatgattcg aagcggccag ctatcagaga tgaaggacaa aaaagaaatc
     1321 gctacaattt tgcgcggaaa aggttgggat gcagacattg caaaatctgt catgaagttt
     1381 gacccaagag gaaacgtttt tcttaacggc acaaaaggtg tgcagtttat ccaagagtcg
     1441 tctgattcaa ttctgtcggg atttgatgag gtaatgaaag aaggcccact tgcaaaagaa
     1501 caggtgcgaa actgcaagtt tacatttact cactttgtac cgcacgagga ttcggcacat
     1561 aggggtctct cacagctaag cccagcatca agaagagcat gtcttggtgc aatgctaaag
     1621 gcacaaccaa tactgctaga accaatgctt ggaatagagg ttcgtgtccc gcaggatttg
     1681 attggagacg ttgcatcggt tctttccgga aaacgaggca aggtacttga tatgcaacaa
     1741 aaaggcatta ctagcattgt aactggtgag cttccggcat ctgagacatt tgatctgtca
     1801 gagactatga ggggccagac tgcaggaaag gcaatgtgga atactcactt taaggcttgg
     1861 actgcaattc caaaatcgat tcagacaacc ctgattgctg atattagaaa gcgaaaagga
     1921 ctgtcacctg atccgccaac tgcagacgag tttctcgata gcgagtaaaa ataacgccaa
     1981 tcatatttgt actctagatt attttctaaa cataatccaa atccttgaac caacaaaaat
     2041 tccaacggct gcaacaatca atatcggaat cagatactga ccaagttcat tttccacaac
     2101 atatgttaaa acctaaatca taaaaatggt ttttattatg ctgttactaa agcgcaaaat
     2161 taggctagaa gaaggttcta ttcctgaaat taaccaaaac tgattttacc caattcgcta
     2221 ataatttaat cctagtattt atctgtctgt tttgacgatt tttattcgtg aaacgcctaa
     2281 cattattgct actttcctca attctcatat taatttctgt aaatgatcaa gcaaatgctg
     2341 caagtgttcc agacccgcca acaaatctta cagcaaccgc agtctcacca acccaaatca
     2401 acctcttttg gtcagcacca tcatacaatg gtgattctgc catcacaggc tacaaaatag
     2461 aatacaaaat cggatccggc gcttacacta ccttggtagc aaatactgct aacacctcta
     2521 ctacatattc tcacgtcgga cttacaacag accagtcata ctcttataga atatctgcaa
     2581 ttaatgcaat tggaacaagc acatcttcta ctgaggcttc tgccacacca acatcatcat
     2641 cttctggaac cttacctggc tctccaacaa atctttctgc aatacctgtc tcaccaaccc
     2701 aaatcaacct cttttggtca gcaccatcaa acaacggtgg atatccaatt acgggctaca
     2761 aaatagaata ccgagtcggc tctggctctt attctactct ggtgtcagac actggcagta
     2821 catctacaac tttttcacat acgggactga caacaaacca agcttacctt tatcgtgtat
     2881 acacgattac ctcatttggg acaagtgagg atccgtctcc agaagcattt gcacaaccaa
     2941 agtcaacatc gacactaaat acaccgagtg ctccaacaaa tcttacagca accgcagtct
     3001 caccaacaca aatcagcttg tcttggactg ttccctcaaa caacggaggc gcagtaatta
     3061 caggatacaa aatcgaagtc aaatctggct ctggctctta cactactttg gtctcaaaca
     3121 cacaaagcac aataactacg tattcccata ctggacttac tactggaacc acttacacat
     3181 acaaggtttc cgcaataaac tcagctggtg ctggcacagc atcaaatgaa tcatctgcaa
     3241 ctccaacatc atcctcatca tctggtgtcc caggagctcc cacaaatctt agcgccatgg
     3301 cagtatctgg aactcaaatc aacctgtcat ggaatgcccc atcaaacaac ggaggatatc
     3361 caattacggg ctacaaaata gaatacagga tcggctctgg ctcttacact actttggtct
     3421 caaacacaca aagcacaaca actacatatt cacacacagg tctgacctct ggccagctct
     3481 atgtttatcg cgtctctgca atcaactctg ctggcactgg cagtccatca tcagaatcat
     3541 ctgccacacc aacatcatcc tcatcagcta gtactccggg cacgccaact ggctttacag
     3601 caacaacagc ttcgccaacc caaatcaacc tgtcctggaa cgccccatca agcaacggag
     3661 gctctgccat tacgggatac aaaatcgagg taaacagtgg ctctggctct tacactactt
     3721 tggtctcaaa cacacaaagc acaacaacat cgttttcaca tactggattg acaacgggca
     3781 ctacatacta ttaccgtgtt tcagcgatta atgcaattgg aactggtgct tcaactgagg
     3841 cttctgcaac accacaagaa acaacgactc caacacttac tgccatggct gtctcaccaa
     3901 catcgattac tttgtcatgg gcgccgccat cacaaacata caagcaagca ataactggat
     3961 acaaaataca ggaaaaaata gctacagata cttttgttac tatagaagaa agaaccatct
     4021 cgactgcgac cacttactcg attactggtt tgacaactgg gaacacatac acatacgtag
     4081 tttcagccca ttttagcttg ggcgcaagtc caaactcaaa cgaagcatct gcaacaccta
     4141 caacaacttc ggcaccacca ccttctcagc caacaacaaa ccctcctagt gcaccaacaa
     4201 acctttccac aaccccaact tcgcccacac aaatcaacct gtcctggaat gccccatcaa
     4261 atagtggtgg ctctcctata accggataca aaatcgaggt aaggagcggc actggctctt
     4321 atacaacatt gactagcaca caaaattcaa ctaccaaata ctcccacacg ggtcttacga
     4381 ctggaacgac ctattcgtac agagtctccg caataaattc tgttggcgta ggttcaccat
     4441 cgtctgaatc atctgccaca ccaactacca cttcggtatc gccaccttcc caaacagcca
     4501 cacctagtcc gcccacactt gccgcaaccc tgatttctcc aacacaaatc aatttatcgt
     4561 ggaccgcaaa cactggagac tctgccataa ctagttacaa aatagaatac aaaattggtt
     4621 ccgaccagta tcgtacttta accagcaaag caactactat cacatactca catgctggat
     4681 tgttggcaaa cacatattct tacagagttt atgcaatcaa ttctgcagga acgagttctc
     4741 catcaaatga ggtatctatc aaaacatcag aacaaacatt gccaccacta gaaccggagg
     4801 aaacaatgtg cggccccgga actgttctta acgagcaagg agtttgtgtt ttacaagagt
     4861 ctgaacctga gccggagcaa tcagaaccaa aggagactca cattccaggc tttccagatc
     4921 ccgacaagga tccacaatac tatatcaaca gatacaatac agaatctaca tacaaagaat
     4981 ggtttgacag aaactttcca gatcagacaa tctatgaggt ggtgggggtg ccagagccag
     5041 agccgcaaga gaaaaaatgt ggcacaggta cacatttgga aaatggaatg tgcgttttag
     5101 atgaaacgca aaatcaattc ccactaagtg gctgtcttat tgctactgcc acatatggaa
     5161 cagagcttgc cccgcaggta cagcaactca gggagatacg tgagcacacc ttactttcaa
     5221 gaagttcagg aactgcgttt ttgggcatgt tcaactatgt gtattattca ttctcgccta
     5281 cgattgcgga ctgggaacga caaaatccag ccttcaaaga gataatcaag gtatcaatca
     5341 cgcctatgct ttcgacttta tcgcttctaa acaatgccaa aattgattca gagcaaagtc
     5401 ttctaggcta tgggttggga gtaattctgc tcaatgttgg catgtatttt ggcatcccag
     5461 cattgcttat tttgttcatc agaaataaaa caaaaacaaa atgcaaacca taaacatacc
     5521 atagaaaaga tcataaatta taatcacact aatgatctct gtgaaagtag ttgggttgat
     5581 tcttggaatc tttcttgtct ggccattgct tactggcagt ttttttgcag aagcgaaaat
     5641 cgatgaaccc atggcaataa ccagtatgaa aaaggacata aaggaaatta aaaaaccaag
     5701 tcaagacaag tcagtaaaaa taaccagcat aacaaaattc cggatgcttg caatcttgta
     5761 tgtagttgga gttgaggttt gtgcgggaaa agataagctt tactcgcctg agctaaactt
     5821 gatctcagac agggattcca taactgtcaa agttgcaggg ctaataatgc ccaaagcctg
     5881 caaaactgcc gaatttttca tacgtgccaa taatccaaat tctatcactg ttaactttgc
     5941 aaatacggat tatcattatc ctaaaagatc gccgtaatga ttacaaatta ttaaaaaaca
     6001 cgagatgcga aaaaaagagt cagaccttct atgaggtcac tttgatttct ttttagcttt
     6061 tgacttcgaa gatttctttg cacttttttt tgccatacca ttaagttttc tgcatgctat
     6121 taaggatatg cattgataat tttttttccg taaaattatt tttgatattt tatgttacct
     6181 atgatgtccg gagatctcag taaaatcaga aaaagcagta gagctatcaa accttctaca
     6241 aaagatcggg ccttacacga ttttttttaa ctctgtattg gtgcaagtga aatgatgaga
     6301 gcatcttttg gttggatatt ccggtttgga acgaaaaaat tcggtaaaat tccaaagatc
     6361 ctatattgtt ttaggttttg tgttaagtag tgacagggaa tcatcacatt ataaattggg
     6421 taaatggttg ggtggggttg cttgggcttt aaagtcgcta cgaattttgt agtttttgcc
     6481 tctgtcttgg ttttgcttag tctgggctct tttgcatcgt acgccgaagc tcaaaccttt
     6541 gagatgtacg tccaaaagat gccaccacac tgggaaaaac agtttggcga tgtcctaaca
     6601 aaggcaacac aatactggga ggagagaata cccggcctga aattcggcgt agtcaaattt
     6661 gtggatcagg ccgactttgt agtggagtgg gcaagccagt atggggaagg aaagattggg
     6721 tattactcta ccaatacggc aaacgagtat ggtaaaccaa agcttgcaat ctcacttgga
     6781 tttttcaaag acaaaaaatg gaatctagta tctactgatt atgcattgga aataacaaaa
     6841 catgagcttg gtcatgcaat aggccttcca cacagtgacg acccaaacga catgatgtat
     6901 ccaataattg aaagttatga atcatggcag caatcacacg cggaaaaacc taaagcaaca
     6961 tcaaaaaccc cagaacaaaa tcttcaagct tcaactaagt cgacaattga ctggcaaaac
     7021 aaatcacaga aataccaaaa acttgcaagc gacaaaattt tcaatctgga aacaaaaatc
     7081 gacacagcaa aatcattgct tgactctacg aactatgaaa acatggcagc caaagtagag
     7141 atcgaaaagg cttggactgc attttggtgg gccaaaaaat actatgccaa cgcagaaaag
     7201 atgcagaccg acggaggcgc attcctctta caatccgagt accaagactc gtataaaaaa
     7261 ttcaaatcct catacgatta tgcaaaaaaa ctggaaaaga agctagctga tgtaacaaaa
     7321 taccataaaa aagcctatgg gttagaatat ggtaaaaaca ttgtcataga atcgtaaatt
     7381 ccgaaatgaa aaaggcagaa cttcgtaaga tggtcgaaga atatggaatt ctaaagtcaa
     7441 aatcagacag tgatgatcag gtcaatccca aaatattaaa aaagattaag gaaattgagc
     7501 gcaggtattt ccatgaaact ggacaagatc tatctttatc ataatgtgtc tggcatcaag
     7561 gttttttagg caagcgcctt tgtgtggggt gcttgctaac aagaaaatcg attagtgtaa
     7621 ttattggggg tagcgtagca gcggcaatcc tgttggtggt tgtggcctct gggcaactag
     7681 gtatgcaaga gatcgcctca actcaattaa ttgaagaaca aaacgtttcc gtcatagaaa
     7741 aacaaacaca agaacaaatg caggtatacc ggatcaatac agagtgtgag ctgatttacg
     7801 gccttgcatc gggaagctat ccaaacgggg aaaaattacc agctctaaag attttggatc
     7861 ttgtagataa atatccggag tttggtccat gggaaacaat tctcagagac aatcaaacca
     7921 gagcggagtt tttcaaacaa tcactgccag aagactttgg cgatgtgctg gtaaatgcgg
     7981 taatgtctga atcctcaatc aacccggagc tagacgaaat tgcatcttta gtggcagatc
     8041 cacagggacg cgcaaaacta cagcaggcat tccaagagtt caactgcaag gcttattttg
     8101 atgaaagagc aaactagtcc tgaaaatcat cacaactttc aaaaacccaa attaaatcag
     8161 caaccagtaa atgcgatcat tacacatctt gataatttcg ataattgcca tagttgtcag
     8221 ctattatgtg attagtgcat ttgttctgca acaacaggca ataaacaaac aatcagtgta
     8281 cgtgcaccta cagccagaat gggatagcta cccaggaaat atcgtttatg acattacgaa
     8341 tgtctggagt caaacggaaa ataggcagtt aaactccaag gaaaggctag aaatgtcaaa
     8401 aagcacaaac gtcgatgaac tgcggtatgt ccatggaaaa tcctacattt tagtccagca
     8461 tgacaacaca aactgccatg attcgtggga gcctcactat gccagatttg gcgcagatgt
     8521 aatacggcac caaatagagt acttgactgg cctgcagtca aatcctgatc caaacgtcac
     8581 attgtacaat ccaatcccaa gcaaacaaga cacaacagaa cacgaatcgc aaataaaact
     8641 gggctattct cagtttatcc caatttgtac tgaaaaagat actacctcct ttgattattc
     8701 ggttagaata aacgataatt cagttggttt tgatgtgtat tttgttccat ctatacaaga
     8761 acaagaaaac tatgatcaaa acaacaacaa attccaatat tatgagacgg gacagtgctc
     8821 tggcaaaaac tatgctagtt tttctggcac ttgttatgat gtgaggaaag actcgggcct
     8881 cctaatcgta attccggata atcttagtct tcctctaaca aagcttgatg tctggctgta
     8941 tgaaaaataa tgtcaaaaag atggctctaa aagatatgat gtataaaaac aagcgtaaat
     9001 ctggaaaatg gttttaattt gagactaacc aagtcatttc atgaaagcaa catttggagc
     9061 gggatgtttt tggtgcgtcg aggacgtctt tagaagaaca aaaggcgtaa catctgtcat
     9121 ggttggatac agcggtggca gcacaaaaaa cccaacttat aacgatgtct gcaccgacct
     9181 tacgggacat gcggaagtag ttcaagtaga ttatgaccca aatgtattgc catacgagga
     9241 actcctagac gtattttgga attctcacga cccaacccag cttaacaggc aaggaccaga
     9301 catcggaacg cagtaccgct cagtcatttt ttaccatgat tcacaacaag aggcaatagc
     9361 cagaaaatca aaggaaatac tagacgtgtc tggcaagttc ggcaaaaaaa tcgtaactga
     9421 gatccaaccc gcagcagaat tctacaaggc agaagaatac caccagcagt attatgcaaa
     9481 atgcggtatg gcgaagtact agctaatttg tcaatgaata attccttagt gagtctttta
     9541 tagaaaatgc aagattgatc agttgatagg tgggatccag tgccggaatc tgatgaattt
     9601 ttcgtcagac catctatcta aagattttat caatgatgat ttgaagacgt acaagatcat
     9661 gatttttgtt tgttgatatg ctaacaatta ttcaaaatcg tttcatccag cattaccaat
     9721 tttgggattc gatagttaat ttattatttc aaatcaggat gcacaaacct gttgaagtgc
     9781 aagaattgcg gaaaggagta ccataacagg gcatcaaact actgctccac cgaatgtgca
     9841 cttgaattca tggaaaaaca ctcagaaccc tgctagaacg atttatcaaa ggcttgcttg
     9901 aactcttaac tgatgtgtct taggtatgcg tttttactgc tttttgttgt tatgctacaa
     9961 aatgtgtacg cccaggactt gccagtcaaa ttggcagaca gcaccaaaaa aacagttgag
    10021 gttggcaaac agatacagat tgctgctgat ctaaaaaaca accaagacgt caagcaggac
    10081 tttgcgtata ttgtccagat ccaaaatgaa gatggtgtaa tagtgtcgct tgcttggatt
    10141 actgggactc tggcgcctgc acaatctttt acacctgcac tttcttggat accaaacgag
    10201 gagggcaggt atgaggccac aatctttgtc tgggaaagca tcaataaccc ttctgctctg
    10261 tcaccaacac tttctttggg aattgatgtg aatcaatttg cttaatgcgt aaaactaatc
    10321 tgtaaaattt agtttcaatt tgacttgctg aaatactaat gacatgtagc aaaaagaaat
    10381 ggcatggcaa ccttggccac aaaagagacc atgtcttttg ctgaaaaacc aattatttct
    10441 ggaaggggtg attcgggaag actacataat tactaaatca aaaaattaca aaaaaaaact
    10501 ttagttattt ggttttaatt gagatttcct gaccattacc aatcggtaag gtgactgtcc
    10561 aaacattttt cttttttaca atgtactttg aataattacg catgatttcg cggtattttt
    10621 ctggatataa catgttgtca gttgctatga tcccgccaac ccttaggagt ggaagcacaa
    10681 ggtcaaagta tttcttggcg ttttccttgt cagcgtcaat tagggcaaaa tcaaatgttg
    10741 gttttttggg catgtttttt agtacatcaa gtatatgact tggaattatt ttgatatagt
    10801 ctgaaacacc agcacgctca aagttgcgtt ttgcccttat gatcttggaa gggttttttt
    10861 ctactgtcat aataactgga tttttatgac aagaaagaag cgcgtctgcg atatacaaag
    10921 tagaatagcc agtagaggtt ccaagctcaa gtgccttttt tgctttgatg gcaactaaca
    10981 aggcatggaa aaacccacca gtatctttcg taattgcaag catgcggtct tctggcttta
    11041 cgtccacttt tcgtgatttt tccagatgtg actgtctttc tagttctgta agaattggac
    11101 caatcttcat gacttttgtt gtgtgtgttc cattataata tatttgagaa atatgctagc
    11161 aaattttgca agcccgatcc cagcaaaaga tcgattaaat aaattatatt tagctatttt
    11221 ttagttgcaa caaattgaat gcttacaaaa acgcctcact aatagaaata gttgaagaca
    11281 tcgtcgatat cgacactggc atgagatttg ccgcaattat tgacttaaaa gggaacatac
    11341 tggaatcaat catgaaaaag ggcaagactt cgcttaaaac acaaaaagaa gaagagcatt
    11401 tttgcaagca aatcgcacaa aggcgcaaaa tgagaaagga attcgacaag agccttggca
    11461 aggtgcgata tgtccaagta gaacgagaaa aggtaattca attcgtagtt tatccaaaaa
    11521 gaaagaccat ctactttacg atggagcctg agacgagcgt taataaaaaa atggcaataa
    11581 taaatcgagt caaaaaactg acaatgcatc tttaaaaatc atctgtttgt tccaaacaca
    11641 cgctacatta caaaacatgg gatccatcaa cttgcatgga ttcaaataat atggttctca
    11701 tacttggtac ggcagtagca cttgctgtta tactgcctct catcataggc tctcttacgg
    11761 gttaagttta aaaaataaat acacaagaca ggcgttctag ttcatcacat gaaaatactt
    11821 gttgatgaga tggatgatgg atgggatgaa aaactccgca ctctgggcta tgaggcatat
    11881 tcggttaaaa aactgctaac tgaaggcaaa aaactacata ctgactattc tgtgataaac
    11941 tacgcaaaag aaaatgagat gatactaatt acgcgtgata cagaatcggg tcaagcatgc
    12001 accgaaaaca acctaccctg tattctgctt gataataacg agatattcaa gattgtgctt
    12061 gaaaagttaa agggttttgt ctgatactag aatttttatc ctgcaattgg attagtaaac
    12121 catggaaagt tgtgacagaa gaacccgagc atacaaaaat ggtaaaacct ttgatcaatg
    12181 tgtgcagatt gcagaggcat taaacccaga gttcaaaaaa acaattgaaa cacagggcaa
    12241 ggttctatgg acagaaattt tatccaaagt ggaccacgac gagcttgtct acaagctgac
    12301 actaaaatac ctacggcggg acggatatga cattgggaac tggcttattc cagaagtcaa
    12361 aaaattttca agttaggatt ctactttaca gctccagccg ctgcttttga tcttttttgc
    12421 catgattact ggcgtaaacg caagcagggg aattgaaact gcaatcagta ttgtctgaat
    12481 ctgagttatt gccaaagtaa acgcaatagt gccagatgcg gaaacaaaaa gtgaaagaaa
    12541 tgtacctgca catgtggggc atgcaatgaa taatcctgtc gttgcagcta tgcttcctat
    12601 accagttcct tttttgagaa ttgaaaaggc gtttacggca atcgcagcgt ttaatcctac
    12661 tagataagag acagtaatct gcaaaattag gttaattggg attatttgaa gacctacatg
    12721 ctctgtcatg taaaagatga gttttggcac gtatcctaca tcgccacagc acggggcaat
    12781 ttgtgcagac ggcacaacca ctccataatg ctttgagaaa ataacatcgg gctgatatac
    12841 tagaatgcca gatgttaggg caaagaaaat accataccca atacttgttg ccacaaaaat
    12901 tctccttgat tttttgttcc aagttacctg tgctatgatg gaaaatacgt catctttgcc
    12961 tgcctgaacc ttttggtggt ggtattggaa aaacccatat gcgattgccc caagtgccaa
    13021 gatgagcata aaataaaatg caacaccgag tctctcaatt gaagatattg cagcaggcgt
    13081 gatctcagag tttccaagac gtgcataaat taaaagcaaa attgcgacac tgacaaatcc
    13141 tattgcaata agtagcctgc catcacggga ttctgccatt tagagtttag ttgaaaagta
    13201 ccatcattaa atcgttatcc acatggccac acactcgttg ttttcaaata tgagagcgca
    13261 acctctttta aattgtgata catacaagat catatgaaaa gcggtacaaa aacaaaaaaa
    13321 cgcacaattg ccacagcaga aatccctatt tcggtgtgtg atgtcatgaa ggacaatacc
    13381 tcgaaaataa tcaaaaaact agaatcacaa attccatttt cgacacagca ttattttgat
    13441 ctttatgcag cattccttca ttctattgat gatgtctttg gtacatgcta tattgcggaa
    13501 aagcagtttt ttgataagat tgggtttgat caaaacatgt taaaatcaat caagtatgtg
    13561 tctgatcagt ttactaatgt actactgaca caagttgaga tgacaagcaa tttgcaaaaa
    13621 tcacatctcc aatttcaaaa gagtgttttg ggcatgtccg aacagtacac tcattcattt
    13681 gttgactcgt actggaagtc attgacagaa cttaacaggt ttttagaaaa tacaaaaaag
    13741 acgtacaaac ttgagccaca aaactagcat tacgaccata aatcctgcaa cggaagaacc
    13801 aatctctact tatgattcga tcacaaagga gcaggtccaa aatagtgtgg caaaagcaaa
    13861 ggctgcattt ggcagctgga aaaatactct agaaaaacga aaaagctgtc tttacaatct
    13921 tacagactat ttacgaaaaa acaagatcaa acttgccgaa gctgccacaa gagaaatggg
    13981 taaggtcata aaggaatcaa tttcggaggt tgaaaaatgc gcgtgggcac tagagttcta
    14041 tgcggatcat gggccaaatt tcctttcaga tgagatactc aatactgatg cgcgaaaaag
    14101 ctttatcacg tttgaaccgc ttggcgtcat cggctcaatt atgccgtgga actttcctta
    14161 ctggcaggcg ttgcgattta ctgcaccaag tctgattgca ggaaacacga ttgtcatgaa
    14221 accctcaagt gttacaattc aatgtggtct ggaaatggaa aaagcttgca gagaagcagg
    14281 ctttccagac ggcgtgtttc aaatattggt tggaagttct ggcgtagcta cgcatctaat
    14341 cgaatccaat gttaatgcgg tgacgtttac tgggagtaca aaggtcgggg cattggtggg
    14401 ccaaaaagct gcctcacttt tgaaaaaatg tgtattggag cttgggggaa gtgatccgtt
    14461 tatcgttctt gaggacgcaa acgtggaaaa agcggcaaac ggcgcagtaa aaggcagatt
    14521 cataaattgt ggccaaagct gcattgcatc aaaaagattc ctcatctcaa agaaagtagc
    14581 caacgaattc atagaaaaat ttgttcacaa cgcatcgcgt ttgcagatgg gtgatccgat
    14641 ggtaatagaa aacgacattg ggccacttgt caacaaggag gcgctagaaa acatttcagc
    14701 aatagtagat gatgcaaaaa agaaaggggc ccaagttcta cttggaggag aaagaatggg
    14761 cgacaaggga tattattaca agcctacaat tctgaccggc atcactgccg atatgcggat
    14821 ttccaaggaa gaaacatttg ggccagtagc accaatcacg gtttttgagg atgaaaacga
    14881 ggcaattcgg ctcgcaaacg acagcgaata cggtcttggt gcaagcattt ggactgaaga
    14941 cctgaacaaa gcagagatgc tctcaaaacg agtcgaatcg ggaattgtta cggtaaacaa
    15001 cgtcgttatc tctgacccaa gaataccgtt tggcggaatc aaaaacagtg ggtatggtag
    15061 agaactttca aaatatggga tgctagaatt cgtcaatatc aagtccgtca ggttttacga
    15121 tcaactgatt catcatcatt atgtggaata aatccaagaa cgacacaaat cgcatatcaa
    15181 taaaatgaaa aaagatagaa aaatcttcta ctatacacag cagagtgatt ttttgaaatc
    15241 atcgacgctt cttgtgaact tgcattcttt gcatacgtgc caagttcgct cttttaatat
    15301 tttcaaaggc gcatcgtagc ctattgagta cattttcccg ccacatgctg gacatggtaa
    15361 tggtatgtta atttgcatca agttaatttc gtgttttgta gatttaagag ccgcgtagga
    15421 ctgataagtc atccatgttt ttcatcagaa cagtgatttc tttgtgaact accactcgct
    15481 aaagctcgtg gcttcctgct tccttgatct cgatatgatc tccacaggcg taacttcccg
    15541 caattcctgc ggtaatttga tattgaaaat atctactaat ccatttttca aaatattgat
    15601 gcttgcgtta tggtccctgt ccagcaccaa gccgcacaca tcgcacttat gcgttcgaac
    15661 tgcaagggtt tttggcacca tgttgctgca tctagaacaa tttatggtag tgtttcttgc
    15721 cggaacctcg actaccattg ttttatagta cagcatattt gtgaaggttc cccatccaga
    15781 atccaagata tttctagcca gtttgtggtt ttttaccata ttcagttttt gtagcctttc
    15841 cagaaatacc acgtcatatt tttttgcata ttgtgttgag attttgtgca gaaaatcctt
    15901 tcttttgttt ttgattcttt catgaattat ctggtagaat ctggttgcct tctttctgtt
    15961 ttgtgaacct ttttgccttc ttgatatctt tctctggatt ctgcgcaatg gttttagcat
    16021 tttggaatag ttcagaggat ttggagtcac aaatccatca gaatcgtata cgaaattttt
    16081 gatgcctaca tctattccaa ctgattttcg aagtgatatt tttggaatta ctgcatcaat
    16141 gtctactgtg atacaggcat gccatctgcc tgactttgat tttgatactg tgatctgttt
    16201 gatgatgtga ttttctggaa tggttcggtg ttcaacgatc ttgattttgc ctatttttga
    16261 caaatgtaaa aaatcgtctt tgatttcaaa tcctgactgg ttgtatgtga aggtacggta
    16321 ttctaagaat ctggcaaatt tgagtgaacc tgtcttgtgg ccgttttttc ttagctggac
    16381 tagtgctttt tgtgcgccgt cgacctgtgc tgatatcgtc tgcagcatct tggagtgatg
    16441 tgaatacaac cacgattcgg attcttttag ttctgtcaga aaatagttga gatcgtttcg
    16501 gctctggaat ccctcggagt ggattttttc caccatagta ttatagaccc acctgcaggt
    16561 gccaagtgta tgatgtagtt gttttatttg ggtatgattt ggatacagac gaaacttgaa
    16621 ggtcttttgc aataattgtt gtatggtttt ttgtctaaaa acagttctga tccgatccaa
    16681 gctgagctgt ggtgtactcg ctttcattcc agccttgaag ggcttgcgat ttcccgctcg
    16741 tatttataag aaatcaaatc catagaaaat atagtgaaca taaaccaaaa ggaggagcca
    16801 gactatgcta cttcaaaaat tgcatatgtt ggttttatcg atccatatgc actagaagga
    16861 ataatcgtcc tacagtcaga tgacaaaaaa gagtttcaca tgagggcttt ttctggcgag
    16921 gtcgcacggc acatcaaaag ctttgttgag ggaaaacgcg acacccttcc aaccatttac
    16981 aatctaattg agcagctgtg cgaagaaaac gaactatatc tagtcaaagt aaaagtgtat
    17041 gagagtggaa gcgtactgcg tgcaaatctg tactttactg gcaaaaagga ccttgtcttg
    17101 cgaaactata gggcgtctga cgcagtggca ctaggctcat tttacaacat tcctattttg
    17161 gtaagaaagt cactgctaaa agaaagggtc gaagcctagc ttagtacttc ggcaaggcgg
    17221 ccttgttctt gtgctgactt gagctccatt gctattctcc taccaacccc aaatgtctgg
    17281 ccgtattggt attttgtgta tggtgaggtg gtagcgacaa ttgggtttcc tgggactcgc
    17341 agcgacacat catacactac tagatccaag tcttttgtta tcacgctttg aagtgagaaa
    17401 ggcccgataa ttccaggcgg atagactttt ttgacgccag caacaaactt gtcacccata
    17461 gctattactt tttcaagtag tgattcgcgg atgcttgcag gcgtgtggcc tacttcgatg
    17521 ttttgtatgt cgatatctat gtcgagctgc tgtcttgcgg ggattgcgtt atagtcgtgc
    17581 aggtttgtct ggagtcgcct ttcaattccg ataaagtcaa cttgatctga aatgggtgtg
    17641 tgaaaataat taaagttcat gtaggtgcca attaccaatt cttcaatact tgctgccttt
    17701 agatcttctt ttctgatgat tccctttttg attcggtctt gggatttttt gagaaaatca
    17761 tcatacgatg aagccgtaaa aaacgcacgc tccaaccttc ttgctttttc ttgcactttg
    17821 acaattacag gcctgtctat tttgcgggga tttttgaaaa gctttgggtg tcggattttt
    17881 gatttttcta gaagatagta ctggttcttt gcaaaactgc gctcttctgc ttgaaataat
    17941 gccctgttgc caaaaattgg tactttgaac ttgttttcta tggtatcgta tcccaaatat
    18001 gctgtgagtg cacgatgggg gacaattatc gtgtttgtgt ccctgaatag tttttgcatc
    18061 ttggcagatg ccatgtcttt gaaattgttt accatgatta tctcgtctgc aattcttttg
    18121 aatcttcgat atggaacatc acgtcccttc tgacagatta cgactgtctg aattccctca
    18181 tcttttgcac catccatgat ttcaagagcg gaatgacttc caagaacgcc aattctaaaa
    18241 tcggaataat tattggcgat tttttgaatc tcagatggcc ttatcatcta caatttgact
    18301 gcaaggcatg gtaatataat taaagacaca cgatatctta aaaaccgccg ctaaattcta
    18361 aaattttgga ggaccagatg atccatttgc caaatccata gttagtatgg caaaaatcaa
    18421 atatctgtag ttcttgagtt aacgccagat ttttttaaca ggggattttt ccaggatgtg
    18481 atctacgaaa attattcgat catttcatat tgattgtaaa aaatttagtg ctttactatg
    18541 ataaatgaaa atactagaaa gaaattaatt tctatatagt atatgtggtt ttacctaaag
    18601 tccataatct atggtggaat tacagaaata acttcgataa acaggtcaga atatgaaaat
    18661 cttggcagca acactaatga cagttttaat tgcatctgtt ttttctcaag ttgcaatcgc
    18721 acaaaaccag tttacgatta ctggagcagg tgcgacattt ccgtttccac tactcgattt
    18781 gtggcgtgta aaatacaacg aggcccaccc aaacataaac atgaattacc aatctattgg
    18841 tagcggtggc ggagtaaaac aacatgttga attaacagtc aattttgcag cctccgatgc
    18901 gccactaact gacggtgaaa tggaacttgc accagactcg ttaactattc cagaagcaat
    18961 aggaggaatc actgtaacat acaatatacc agaaattcca caaagtggac taaaacttac
    19021 aggccaacag gttgcagata tttttcaggg taaaataaca aaatggaatg atgcacaaat
    19081 tcagcaaacc aacccagacc tacctctacc agatgaaagt attcttgtgg cacaccgctc
    19141 agacggttct ggtaccacat acgcctttac tgaatacctc actatggtga gtcaagattg
    19201 ggatgatcaa gttggctttg gcaaaagtgt tccttggccg gcaggagttg gggcagctgg
    19261 aaatgaaggc gttgccacca cagtaagaac atccccatac tcgataggat atgtggagtt
    19321 ggcatatgca ttccaaaata acatggcagt tgcattcgtt caaaacgccg atgggactgc
    19381 atttgtagag cctacatttg atactttgtc tgctgctgcc gccggtgctg cagcaggatt
    19441 gcctgcagcc cacgaacatt ggcaagaagt cagcattaac aactcgcccg gaccaaattc
    19501 atatccaatt gtaagtttca catacatact cttacaccaa aacttggagg ctgctacacc
    19561 aagtaaggca catgcacaag aaacagtcaa tcttataaaa tggatgataa ctgaaggtca
    19621 gaaatataat cctgaattac tgtatgttcc aatcccagct gaagtaacac aactcggttt
    19681 ggatggcttg gacagagtca catacaatgg ccaaccactt tttgcagagg cagcaatgga
    19741 acaaccgcaa gatacacagg atgctgaagg tggtggatgc ttaattgcaa cagcggcgta
    19801 tggctctgag atggcatcac aggtgcaact acttagagaa gttcgagaca acgtactgtt
    19861 cagcacgact tctggagcaa acttcatgac tagctttaac tcgatctact actcatttag
    19921 cccaactgtt gcagactggg aacgaaacaa cccggcattc aaagaagtag taaagactgc
    19981 aatcactcca atgctttcga ctctgtcaat actcaactat gtagatattg actctgagca
    20041 agaaatgctt ggttatggag tgggaataat cttgcttaac ttgggaatgt actttgtagc
    20101 cccagcaatt gtaattgtga agctacgatc aaaatttaat tcaaaataag agattagtcc
    20161 ttagaaatgg gattcttaaa agtagtgagt ttgttctaat ttaggggaaa aaattctata
    20221 ttgtatccca aaagaatctt gatttaagtt ataactagcc attaattatg aatggtgagc
    20281 tcaactggtc catgaatata gagacagaat ttacatcaga aaggatatcc tattgaaact
    20341 ttacgagcat ggtgagctca accagagcaa attgatgagt tactgcggcc taaataatgt
    20401 aaaacacaaa ggaatcttgg atgatatggt taaaaaagaa atgatagtta ggacagaaga
    20461 gccttgggga aacaagaaca tcataaaata caaaatatca gacaaaggaa aaggaattct
    20521 tgacgaagtt cttggaccat atgaggaatt attcccaagg ggaggaaaag aagatgagta
    20581 acaaagttac tactgtgttt ttaagcaaaa gtcaggaggg tactcagtca tgacggattc
    20641 cgaaaaaaac ggcagtaata tcttcaaaac aatggatgag atcatctttc agctaaacag
    20701 gacaaaaaaa atgttcataa taatgattct tactgttatg attattccgc caattacgtt
    20761 cttgattttt actgcaatat atgatcctcc ttttgagtcg caagaaaaaa cagagcatga
    20821 tggatcaaaa ccaaaattca caatatttcg ggttattccg ctaataatat ctgcagtctg
    20881 gcttggaatc ggaataaggc agtggatagt tctttccaaa tggaccaaaa aatatgaccg
    20941 atacaaggaa ctgcaagaaa aaattgacaa gaaattagac gatgatgaag aaaaagacga
    21001 cgaaaaagat tcctctagtc gatagtattt acaccgtgcg tagcacttat cattaggtaa
    21061 taatgttacc taatgcttta tttggagagc catatacgct acgtgggatg aaccagggaa
    21121 agatacggga ctttagaaca catgttttta gttttaccct gcttggcatt attgtatcgt
    21181 ttgcactagt tctattctca ccatcacaag ccttggcaca agaggtgcaa gtaactaaaa
    21241 taaattccga ttttactgtc gttgcaggag aagagataaa gaaaaaccca actgcgattc
    21301 aaattcttca aaacattgag attgctaaaa aaagactagc ggaaatgcag aatgtagaaa
    21361 aacagaaaac cgaacgtgag aaattcattg atgaacaacg ccgaattgcc aaagaattat
    21421 tggaaaagga tcttaacagg atgaataaag attacgaaga ttatacgcca aaaaatgcat
    21481 ttgctagatt cgtttcagga tttaattcaa ctcatcatgc gatatactgg gatcaatttg
    21541 agtacatgaa ccaaaaaatc aaaattgcaa ctcaggcaaa acaaacagtg ttggcaaacg
    21601 gcggctcgta cgtggaggcc caagcagaat ttatcaagta tgcatcaatg ccacgagttg
    21661 agatgataag ctttgtttcg gatttgaaca taaagtatgg attcactgat gcagcattac
    21721 aacaatactt tgatgagaat ggaaaactac ccagatatga aaatgatgat gttgctgttt
    21781 gttatgcatg tgacaagtat gagcaggtca aagtggaaat gcttgcagaa catgagagtt
    21841 ctaaaaacga atcaacctaa acaaaacatt ttgctaaaat ttcaagtcgt attttcgtat
    21901 agctagacat gttttataac aatcaataat gctgtgctat tcaattcttg cagcttaaaa
    21961 taatcaggca tttctactag tgttatgaga ctggtgattg gaatgacggg aagcaccggt
    22021 gtagtttacg gtgttcgtgc actcgaggtc ctaaaagaat gcaaaattga aacgcaccta
    22081 gtaataacag aatgggcaaa aaaatgcctt gcaatggaga ctgactacaa gtttgacgat
    22141 gtaaaatcgc ttgcgacaag ttattctgat gattcaaacc tcgcatctgg gatatcgagc
    22201 ggcacctaca agacagacgg tatgattgtg attccatgta gcatgaagac actatctagt
    22261 atcgcaaacg gatacgacga aacccttgtg gcaagagcag caggtgtcac actaaaggaa
    22321 tcaagaaggc tggtcctggt tccaagagag acgccgctaa ctgccataaa cttggaaaac
    22381 atgctcaagc tagcaaggct tggagttgtg attttgccac cagttccggg attttacacc
    22441 agaccaaaat caatcgaaca aatcattgac cacacggtag gaaaatgtct tgaccagttt
    22501 gacattgaac ataatttata caaacggtgg gggggaacct aatctgtatt gctaaggttc
    22561 agactagaaa aaataatcaa agcagaaagg caacacgttt ttgatattgt ggcaaactat
    22621 gagaattttg agaaaattct accacaatat ttcccatcaa ttcgaatacg atcagttagg
    22681 gacaatactg ctatagttga agaacatcta aggcttggga gcagagaact agtcatgatg
    22741 actaaacatg tcacgaaata tcctgatgtt catgaagtct ttgtagttgg tggggatgca
    22801 aagggcagcc actttgttga aaaatacgaa aaaattcctg agggaacaaa aatgacagtt
    22861 gacgtcaata tcaagctaaa agggataatg gtaatagttg gtgctttggg taaacataag
    22921 atatcttcgg agtttggcaa aataattgac gagtttgcaa aaattgctga aaaatcctag
    22981 tctttgttct ctttgaggtc tttgagttct ttttcggcct caactttacc tttttcgtat
    23041 tccattcttg atttgcctag cgttcttgca agctctggaa tcttttttgc accaaatatc
    23101 aggatgcctg ctattattat gatgaagatc cattcttggc ctgcaataaa attggcaatg
    23161 tttccgagca tttctggtta atcactagta tgtcaagatt gagatttaag tctttgagtt
    23221 ttgcgctcac gtcgctccaa aattatcatg cctgtcatgt atagtccgat cattgggcct
    23281 gcaataaacc acattgtaac tccactccca tcaggtgtta cggctgcacc aattacgact
    23341 agagcaacaa ttgcatatct gacgtttcgc ctccaaaatt ttacatccgt caggccagaa
    23401 acggttgcag catacatgat tagcggaagc tgaaacgcaa ctccaaaggc aagcaaaaac
    23461 tgcaaaacaa acgtcacaaa atcaaccaca ttaagaaatg tgacaagacc tgctgattcg
    23521 ccatactgat acaaaaagtc caacatgaat ggtattacaa ctagatatga gaagatcagc
    23581 ccgccaacaa ataatgcgat tgctggtagc gcaattgagc ggaccgcgtt tacttcgttt
    23641 tcggacaatg cgggtttgat aaacgatgta agctctcgta caattattgg catcgatgtg
    23701 acaattccgg ctagtgctgc aatgtagact tgggcgtaaa atgcctgacc tggtgctgtt
    23761 tgtatgagct gcacgtctgc tggcacaagg gttgccttca tgtgatttgt tatctgtgct
    23821 gcaatgttgt gtaaagggtc tggtgtcgga tagtaaagtg taattccact caaaacaatc
    23881 tgttctgctc tgaatgtgag gatgaaaact acgatgattc ctgcggcaat taggattctt
    23941 gtggcccttg agcgaagctc agctaaatgc gcatagattc cctcaaactc tgacatgtgt
    24001 gatattatgg cgtgaaactc tatttagttt gcgaaaattg tggctaattt ttgccaggaa
    24061 ttggcagcaa tttggcatct gcaaggccgg aattttttcg ttcttcctct gtaagatttt
    24121 ggaactctat cgaaattatg gcctttagct tgaatttggt ctcaagatcc tttgcaagca
    24181 ggccaatttc tttaccgcta tagatctcct tagagtccac caaaaacatg cgatcccctt
    24241 tttctttttc agttcccccg aatctgtcct tgagaaattt ggttacctcg cctacctcgt
    24301 tttttgtcac attgttgtga taaaaaacaa ctcccttagt cggcacgtaa tcaattggag
    24361 ttccgtttct gtacaaaaac acgccaataa agattgccgc atcatccggc tcttttattc
    24421 cctttatttc ccagtttagg actttgcttt gctcgtaatc tgcttttgaa atctcatcag
    24481 atgttatctt ccagtatcta tgtctagtca cttcagtctt tgactaaata ctggatataa
    24541 attcaatccg atttcatatt tttcctgtcg tagaatatat tacacaccaa aatcaacttt
    24601 gggcatgcca ctttttgaag ttcaagttac gatagaaaac aagcctggaa tgaatgatcc
    24661 cgagggccag acaattctaa acgacctggt actaaaggga agttattcat cggtcaaaaa
    24721 aatacgttca gctaagatgc tcaaattcca agtaaatgct gcaacaaaac aagctgcaca
    24781 aaatacagtc aaaaaactat gtgacgatct tagaatatac aatccaatag caagtaaggt
    24841 tacttttacg gcaacgtcaa gctagtcttg gtccccaatc gatttgacgg tctcatcaat
    24901 tagcagcttg cttctcaaat gtgctgcaag gtctgtctgt gtgattacgc ctgccaagtt
    24961 ttggttttct agtacaacta gtctgcgaat gtggttgttt aacatctttt gaacggcgtc
    25021 ttcgattgta gttgttggtt caacccatct gaactttggg gacattattt cagacacttt
    25081 ggtttgcgag gcgatcttat tggctgctac caattttcgc acaaagtcag cctcgcttac
    25141 gacgccaact ggattttgcc cttcagttac gaccaaaaag ctgatgtctt tttcatgtag
    25201 tagagtagct gcttcaagac atgtcttgtc ttttgatatt gtgattacgt gcttgttcat
    25261 gatgtctctt acatatccca ttattggtct tggttttttt gcagataaaa aagatttgcc
    25321 gcgctttgat ttaataaaga cattgctcaa ctttacttgt gaaggttggc gtcatagtat
    25381 ttcccggtag caattgcgat cgagacatgt ttcatgtcct ccaagatgtc ttcaaactcg
    25441 atgtcaagta tttttggcat gaggataggc taccaaaaaa tttggatgca gttgttctgc
    25501 ctggaggatt ttcttatggt gacagattac gtgcaggtgt gattgcggca cacagtccgg
    25561 tcatttctga tgtgaaaaga atggccgaca aaggaatgcc aattcttggc gtctgcaacg
    25621 gatttcagat actagtagaa gcgcagcttt tgccaggtgt cttgcttaga aacacatcgt
    25681 tgaactttat gtgcagatgg actgaactaa tagtggaaaa taacaagaca ccatttacaa
    25741 accaattcaa gctcaagcag aaaatcccaa ttcctattgc aaacggtgag ggacgatatt
    25801 atgtagataa aacaacactg gctcagctaa agaaaaacaa ccaaatcgta tttcgttatg
    25861 gtgaaaacat aaacgattct gtctttgata ttgccggaat ctgcaacaag gaagggaacg
    25921 tggttgggat gatgcctcac cctgagcggg cagtggaagc cgagataaac ccaaaagact
    25981 ccaaaccctc atccctgatt tttgagtcat taatcacaac agttggtgca aaaagatgag
    26041 cctgcaacca caagagctac agtacctatc aaaaaaaatc ggccgtaaac caaacgaaac
    26101 tgaaacgcat attgtggcag cggaatggtc tgaacactgt tcctacaaat catcaaaaaa
    26161 acatcttaaa atgcttccaa catccgggcc acgcgtaatt catgaaaaag gatttgattc
    26221 tggcgtccta gatgttggag acggatatgt tgtaacagta cacattgaaa gccacaacca
    26281 tccgtcggca gttgagccat acggtggggc ggcaactggt gtgggtggtg tgatccgcga
    26341 cattctttct gctgggacac gaccaattgc aattttaaac ggattgcgtt ttggagatat
    26401 cgaaaaagat tctcatgcaa ggtggctttt caaaaatgca gttactggaa ttgctgacta
    26461 tgggaattgt cttggcattc caaccgttgg cggagaagta gagtttgaca aatgttacac
    26521 aaactatgcg cttgtagatg tggcatcaat tggatttgga aaaaaagaac aactgatcaa
    26581 aaaccacgcc caaaaagacg acatcgttgt attgattggc ggctcgacag gccgtgatgg
    26641 gattggcggc tcgcagtttg catccgactc gctagaaata gaggatcgct ctgcagtgca
    26701 aattccagat ccctttattg agaaattaat aattgaggca acacttgagg cacgaaatga
    26761 agggtgcatc aaggcaatca aggacttggg tggtgggggg ctatcatgtg caatatctga
    26821 aacggcagat gcattgggaa ttggaatcga gcttgatgta aatcacgttc atacaaggga
    26881 gccaaacatg aatccgaccg aaattatgac atccgaatca caggaacgaa tgctcatcat
    26941 taccgacaga caaaagcttg gcaagctaga aaaaatctgc aaaaaatttc acgtaagctg
    27001 cgctgtaatt ggcaaggtta ccggcaacaa attgatgaag gtaacgacta acaagaaagt
    27061 tgttgcttcg atgcccgccg aagtagtggc aaacgcaccg cttcttgata ggccatcaaa
    27121 agagccggca tatttaaaag agctacaaaa aagacaagaa ccaaaaccag ccaaggactt
    27181 gtcaaaaacc cttctaaaac tgctctcaag cccaaacatt gcctccaaaa tttgggtcta
    27241 tggccagtat gatcatgagg tgggaatcag aacagtagta aaaccaggct ctgatgccgc
    27301 ggtgctgagg ctagataacg gcaagtactt ggcagccaaa atcgatggaa atccaaagca
    27361 ttgttatctt gatccaaaaa acggcgcaat tggatgcttt gaggaagcgt gcagaaacgt
    27421 cgtatgtgtt ggcgcaacac caattggaat ggttgatcat ctccagtttg gtaacccgga
    27481 ggatccggag atcttttgga catttttaga atctctaaag ggaatcacgg aattttcaaa
    27541 gttcactaat gttccgtgtg ttggcggaaa ggtgagcctc tacaatgaaa ctccgcaggg
    27601 accgataaaa ccaacgccgc tgattggcgt gttgggtcta attgaaaaaa aaccactaaa
    27661 gccacaaacc ctagagactg gtgacctctt gcttattgtt gggaaaacaa aagacgagct
    27721 tgggggttct gaatattatg aatacatcca tgatttcatt ggtggcaaat gtcctgttgt
    27781 tgattttgag gaatcaaaca aaaacatgaa agctgtcttg catttgattc aaaatgataa
    27841 gataaaggcg gctcatgact gctcaaaagg cgggcttgcc attgcaatct gcgagcttgc
    27901 agtgacaagt aaaattggtt gcaatattac actggaaaac atacctcagg aaaaatcaaa
    27961 tgtgagcaaa ctgttgtttt ctgaaagtca ttcaagatac ttgttggtaa tcaaaaaatc
    28021 aaatattgat gcagttgtcg attatctaaa aaagaacaaa atagcgtttg gcctgatcgg
    28081 caaatttgaa ggaagcgata tcaatttttc agaaaacaac aagccggttg caaaactaag
    28141 ggttgataag gcacatgcca agtggtttaa ttcactgagg gaactggtat tgcatggtta
    28201 aggaaaactg cggagttgtg gggattttta gtcgtgatgg cgtcaatgta attcctatgg
    28261 tcattgatgc actaagggca cttcagcaca gaggccaaga agcatgggga attgcaattc
    28321 ctcaaaaacc accactcaaa cgcctaggtt tggtttctgc agcatcttct gaacttcaaa
    28381 aaattaccca agaatatgca gcacctgcag caattggcca tgtaagatat tcgactgttg
    28441 gaaaaagcaa cctcgataac gcacaaccac tcaaagtaaa agacctctgc attgcacaca
    28501 atggaacaat cgcaaacgta tctgaacttg caaacttggt tgggggatgt acctttaccc
    28561 cacaaaatgc aagtgacact ctgattgcag cacaaaggtt agtctcgcta atgtccacta
    28621 acggcaatct tggaagtgca ctgtcaattc taaaaaatga gatggctggc tcgtactgct
    28681 ttacgtttgt ttcagatgac aacgcagtct atgcggcacg tgatcctagg ggctttaggc
    28741 caatggttct tggctcaaaa gaagaagggc aagtaagcgt tgttgcgtca gaatcatctg
    28801 cacttacggc agttggagca aagcttaccc gtgatgtttt accgggcgaa ctcttaaagt
    28861 tcagcgacaa aggagtcgag tctgaaatgt tttctgagga aaaaaaacat gcgcactgct
    28921 catttgagtt cacatacttt gcacacccaa caagcaaaat ggaaggaaca aacatctacg
    28981 ttgcaagaaa aagaatcgga caattcctag ctaaaaagtt tccaataaag gaagcagatt
    29041 tggttattcc agtgcctgac tctgcaaggc ct
//
LOCUS       DASWKH010000062         6035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1261604, whole genome shotgun sequence.
ACCESSION   DASWKH010000062 DASWKH010000000
VERSION     DASWKH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261604"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGA92_04790"
     CDS             <1..226
                     /locus_tag="VGA92_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845763.1"
                     /translation="ILMDLAMPDFSGPDIIESLYNRNLMNNKLIIALTASSITDEDKT
                     NLKKRGVHSILKKPIDPDELLNYLVKIRGP"
     gene            230..1624
                     /locus_tag="VGA92_04795"
     CDS             230..1624
                     /locus_tag="VGA92_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9845764.1"
                     /translation="MTDLEHLILEFQQIELIQKKLNEMGRSDIPKNKLISQIIDHIES
                     ANNFSASTVEDIVSNKIVLTENTLSIINHELKTPLVPMKAYADMLLQGKFGNLNAVQK
                     EKVTIIASNIKRLQQKIEFLLDKRTLASHTPDDSNATFSAKELKQQNMLLEKINQLLT
                     EKSNKAESEVQKLHNTIIESKHQKNEIKQERVMMNKLVQIEEQKKVRLTKKNLMMMAT
                     AALIVGAGFTTYSVYVVDLVGKQYQVSNLGRMPGGYLIQNLRGDTIDTWLSWRPVSDS
                     TLHVGIMNGAAYPEKIQLIKEVVMSEESIDIDDSLLHKGPEGITSTYYLGWLGALKQA
                     SAKPTEFNIPVNLEVIESSRGEGEITIVLTNDRNADGYSGFTKSIADESQNQILKSTI
                     TIYEVDKLNDEQFKTILRHEFGHALGLAHSTAPEELMAPVIQTEYPYISECVIDSIVK
                     LYDNGKNSQVVCEK"
     gene            complement(1942..2496)
                     /locus_tag="VGA92_04800"
     CDS             complement(1942..2496)
                     /locus_tag="VGA92_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845765.1"
                     /translation="MEKSLAVIVSVILLASACVVYQDAFALEQKQTTDKKPYKDAYSS
                     TKDWLYSIILPEGWNEKTDLPFYYASAQTDGNPVIIGYKVHTQKSFASRDDLHSYLKS
                     TEYLPIGQFGLGNSIMTVDTKYGYKTTIQFKFQKQDGDQIMIRKDFHFMNKEGNLYSV
                     QGFSSDMDSFKEIKKTLDGFRPVT"
     gene            2648..2872
                     /locus_tag="VGA92_04805"
     CDS             2648..2872
                     /locus_tag="VGA92_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845766.1"
                     /translation="MAKKEQKTKKEKAKKPQKKEKAAKKSKSKKTKKGDFIADDGLVI
                     IDEKIEEDKEAELEARKAYLEEARSQEVDD"
     gene            2909..3148
                     /locus_tag="VGA92_04810"
     CDS             2909..3148
                     /locus_tag="VGA92_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845767.1"
                     /translation="MRAVCLITIKPGKTNKVMESLRKKRKLLKEILPVTGRADICVLL
                     QGTIEEINNMVIDFKKIKEIVTTETMIEVEVDMGW"
     gene            3142..3360
                     /locus_tag="VGA92_04815"
     CDS             3142..3360
                     /locus_tag="VGA92_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9845768.1"
                     /translation="MVKAIILVKSPKKLIAAKLGKLSYVYNSFPVSGQFDAVAFVEVD
                     DLTQIRGITTKIQMISGVERTETMLEIQ"
     gene            3392..3805
                     /locus_tag="VGA92_04820"
     CDS             3392..3805
                     /locus_tag="VGA92_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9845769.1"
                     /translation="MNIKRVGNVILAVKDLDKSIAFYHEILGLPIKNQRRTWVDLGQT
                     GALLSLHPASLTSDHSSNTMEGGIVVGFLVGDVKSAIDELKTKNVKIHRDIVERDAGK
                     NVIVLDPDGYMVSLFEPIFRDKDQQSTGYHGFAPA"
     gene            complement(3802..4188)
                     /locus_tag="VGA92_04825"
     CDS             complement(3802..4188)
                     /locus_tag="VGA92_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845770.1"
                     /translation="MIKTPDQKWKEAILGYRKNCQKILGVSKSIRYVGVINEYGRTLA
                     GMMQSGLKPMLKPEDVKNEFFIISNLITLRNSVSKAFGALDHAILFHNNATLVCVPHN
                     KVIYYVSINPKAKSIDEIISKIKNLL"
     gene            complement(4319..4696)
                     /locus_tag="VGA92_04830"
     CDS             complement(4319..4696)
                     /locus_tag="VGA92_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845771.1"
                     /translation="MNPAETSTFMEMYGYPFGYSLSPFAFPRYPPSAIGPMSMNPAKL
                     KEKMIKENSTFSAKLHDFSKLYLKHASGLLDMSSNIFPPGHPMNVPSRVDYTIQEENE
                     KLRKENAELKSRIEQLAKTKKQF"
     gene            complement(4981..5745)
                     /locus_tag="VGA92_04835"
     CDS             complement(4981..5745)
                     /locus_tag="VGA92_04835"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9845772.1"
                     /translation="MKPKTDYDIIDMMLSKPSERAVDSDTLIKETQNRVWRALKSGYE
                     YSPVEDESDAFLRKHVFSRMRMVILYVDLVGSTQITLELPEDKVAIIITCFAQEMAAT
                     IHHHNGYVLKFVGDAVIGYFPAEENQLQPADNAVLCAKSMLSVIQKGINPILNQYDYP
                     DLAIKIGIDYGQNMIVRYGADAKKSHVDILGPVMNIAAKIQNMAKPNQILIGDDVYSR
                     IHPSMQAAFEKVVWKNSEWKYHRETGELYPVHKYVD"
     gene            complement(5815..5937)
                     /locus_tag="VGA92_04840"
     CDS             complement(5815..5937)
                     /locus_tag="VGA92_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845773.1"
                     /translation="MSVTKIICLSCNVEVVQYYDDAYKGKRAKCPICDVSFPLE"
BASE COUNT         1964 a         1094 c         1188 g         1789 t
ORIGIN      
        1 aattctcatg gatcttgcaa tgcctgattt ttcaggacca gacataatcg agtcccttta
       61 caaccgcaac ttgatgaata ataaactgat tattgcatta actgcatcgt ccattactga
      121 tgaggacaaa acaaatctta agaaacgtgg ggtacattcc atactaaaaa aaccaattga
      181 ccctgatgag ttattgaatt acttggtaaa aattcgcggg ccgtaaatta tgacggatct
      241 ggagcattta attttagaat tccaacaaat tgaattaatc caaaaaaaac taaacgaaat
      301 gggtcgtagc gatatcccaa aaaataaact gatttcacaa ataattgatc acattgaatc
      361 agcaaataat ttttcggcaa gcacagtaga agatattgta tcaaataaaa ttgtactaac
      421 agaaaatacg ttgagtatca tcaaccatga attaaaaacg cctcttgttc caatgaaggc
      481 atatgccgat atgttactac aaggaaaatt tggaaatcta aatgctgtac aaaaggaaaa
      541 agttacaata attgcttcaa atataaaacg attgcagcaa aaaattgaat ttttactgga
      601 taaaagaacg ttagcaagtc atacgccaga cgatagtaat gcaacatttt ctgcaaagga
      661 gctaaaacaa caaaatatgc tgctagaaaa aattaatcag ttacttactg aaaaaagcaa
      721 caaggctgag tctgaggttc aaaagttaca caatacaatt atcgaatcaa aacatcaaaa
      781 aaatgagatc aaacaggaaa gagtcatgat gaataaatta gtacaaattg aagaacaaaa
      841 aaaagtacgt ctcactaaaa aaaatctcat gatgatggct acggccgcct taattgtagg
      901 agcgggtttt acaacatatt cggtatatgt tgtagatctc gtaggtaaac agtaccaagt
      961 ttcaaatctt ggaagaatgc ctggcggcta cctaattcaa aacctaaggg gcgacacaat
     1021 cgatacgtgg ttatcatgga ggccagtttc tgacagtaca ttacacgtag gaattatgaa
     1081 tggtgcggca tatcctgaaa aaattcaatt gatcaaagag gtagtcatgt cagaagaatc
     1141 aatagatatt gatgattcat tacttcataa agggccagaa ggaatcacat caacatatta
     1201 tcttggctgg cttggagcac taaaacaggc atctgccaaa cctaccgaat ttaacatacc
     1261 agtaaatctt gaagtaatag aatcatcaag aggggaaggc gagataacaa tagttcttac
     1321 taacgacaga aacgctgatg ggtattctgg atttacaaag tcaattgcag acgaatctca
     1381 gaatcaaatc ctaaagtcca cgataacaat ctatgaggta gataaattaa atgatgaaca
     1441 gttcaaaaca atcctaagac atgagtttgg ccatgcattg ggtcttgcac attcaactgc
     1501 accagaggag ctaatggcgc cagttattca gacagaatac ccatacattt cagagtgtgt
     1561 tattgacagt attgtcaagc tgtatgataa tggaaagaac agtcaggttg tctgtgaaaa
     1621 ataatatttt agaattaatt tgaaaataaa tctattttta ctattctaca ggcagtatgc
     1681 tggttgatcg caagatctag acatttaaga ttttaagata gtcatattta caggaagaaa
     1741 aaatgcaaaa tctcattcca caactatatt ctccctcaaa acgtgcgggc agtaaaattt
     1801 gaaccaaaca gtatacgata ttgagtattt tagaaaaaag ccatttgatg agtggggtac
     1861 ataataattc gctaaaatcc cacccttggc acatattttt attatgtgca ttcaaaatat
     1921 gagaaaaaga ttttgactaa tttatgtgac tggcctaaaa ccatctaggg tttttttgat
     1981 ctccttaaaa gaatccatat ctgatgagaa tccttgaact gaatacagat ttccttcctt
     2041 attcataaaa tgaaagtcct ttcgtatcat aatttgatca ccgtcttgtt tttggaactt
     2101 gaattgaatt gttgtcttgt agccgtactt tgtatctaca gtcattatgc tgttgcctag
     2161 cccaaactgc ccaatcggca aatattctgt tgatttcaga tatgagtgaa gatcatctcg
     2221 acttgcaaaa cttttctgtg tgtgtacctt gtatccaatg attactgggt ttccgtcggt
     2281 ttgagcactg gcatagtaaa acggaaggtc tgtcttttca ttccatccct ctggaagtat
     2341 tattgagtag agccaatctt ttgtgctgct gtaggcatct ttgtagggtt ttttgtcggt
     2401 tgtttgtttt tgttcgagtg caaacgcatc ttgataaaca acacatgctg aggctagtag
     2461 aattacagaa actattacgg ctagtgattt ttccaagctt gactagtttc gcagatcttg
     2521 cctttaaatc ctgctatcaa ttgcgtttga caagattatc cttaagtacc taatttttga
     2581 agttggaaag attcctaaaa aacaagtaat gttttaatac atgagaatgg tctgatgaga
     2641 aagtcagttg gcaaaaaaag aacaaaaaac aaaaaaagag aaagcaaaaa agcctcaaaa
     2701 aaaggaaaag gccgcaaaga aatcaaaatc gaagaaaaca aaaaaaggtg atttcatagc
     2761 agatgatggc ctagtaatta tagacgaaaa aattgaagag gataaagagg ccgaacttga
     2821 ggcaagaaag gcctacctag aggaagcaag gtcgcaagaa gtagacgact agcaaatctt
     2881 tattcataaa ttcattctat actaacgcat gcgtgcagtt tgtctgatta ccataaaacc
     2941 gggtaaaacg aacaaagtca tggagtcatt acggaaaaaa cgcaaactcc tcaaggaaat
     3001 actgccagtt accggccggg ccgacatctg tgttctgcta cagggcacaa ttgaggagat
     3061 caacaacatg gtgattgatt ttaagaagat caaggaaatt gtcaccactg agactatgat
     3121 agaagtggag gtcgacatgg gatggtaaag gcaataattt tagtcaagtc accaaaaaag
     3181 ctgattgccg caaaactggg aaagctgagc tatgtataca actcgtttcc agttagtgga
     3241 cagtttgatg cagtggcatt tgttgaggta gatgacctca cacaaatacg tggaattacc
     3301 acaaaaattc agatgatttc cggtgttgag cgcaccgaaa caatgcttga gatacaataa
     3361 agaatttaat gttgtgctgt ataaccaacg tgtgaacata aagcgggtcg gaaacgtgat
     3421 tttagctgta aaagacctcg acaaatcaat agcgttttac catgaaattt tgggcctgcc
     3481 aataaagaac cagagaagaa catgggtaga tttaggccaa acaggcgcgc tgcttagcct
     3541 gcatcccgca tcactcacat cagatcacag ctcaaacact atggaaggcg gcattgtggt
     3601 tgggtttcta gtaggggatg taaaatcggc tatcgatgag ctaaaaacaa aaaatgtaaa
     3661 aatccaccgt gacatagtag aaagggatgc tggcaagaac gtcatagtct tggatccaga
     3721 cggctacatg gtatcgcttt ttgagccaat ctttagggac aaggaccagc aatcaacagg
     3781 ttaccacgga tttgctcctg cctagagaag atttttaatt ttactaatta tttcgtcaat
     3841 tgattttgct tttggattga ttgatacgta atagatgact ttgttgtgtg gaacgcaaac
     3901 aagtgttgcg ttgttatgaa atagtattgc gtgatccaaa gctccaaatg cttttgaaac
     3961 tgaattacga agtgtaatta ggtttgagat gataaagaac tcatttttca catcttctgg
     4021 ctttagcatc ggttttaatc ccgactgcat catgcctgcc aatgttcttc catattcatt
     4081 gatgacaccc acatagcgaa tggattttga aacaccaagt attttctgac agttttttct
     4141 atatccaagt atcgcttcct tccacttttg atcgggtgtc tttatcaaca actaaccaac
     4201 taaatcaaag ttaaaagagt ttttgattgt tatttagtaa aacattcatc aacctaataa
     4261 caaatagatg gccatctcat aaggcatgac aaaaacgaat ctgataaatt tcgaaaatct
     4321 agaattgttt tttggttttt gctaactgct caatgcggct tttgagttct gcgttttctt
     4381 tgcgtagttt ttcgttttct tcctgtattg tataatcgac cctacttgga acgttcattg
     4441 gatgtcctgg tgggaagata tttgagctca tatcgagcag gccagaagca tgtttgagat
     4501 agagtttgct aaaatcatgt agttttgcag aaaatgtcga gttttctttg atcatttttt
     4561 cctttagttt tgcagggttc atactcattg gcccaatcgc agatggggga tatcttggaa
     4621 atgcaaatgg gctcaaactg tacccaaatg ggtatccata catctccatg aatgtgctcg
     4681 tttctgccgg attcacacgt attggtattt tagaagacta tttttgcctt tagcatcagg
     4741 cgtaacatat acccagttgt aaagccggag tcatttttta tcaaattgag ctgatcgcag
     4801 atccttatag cgatcaaaga tcgcccatca gatcatcatg ggatttgatt tgaagcattg
     4861 tatgaaatgc aaaaaaaaca tcagtgttgg cgaattgcat aaaattgtaa tttatgtgat
     4921 tcaagaaaaa ttcacggacc accactacga gcacgtagag tgtcctgaca agtttactat
     4981 ctaatccacg tatttgtgga ctggataaag ttctcctgtt tctctgtggt acttccactc
     5041 gctgtttttc caaacaacct tttcgaatgc agcttgcatt gatggatgaa tcctagagta
     5101 cacgtcatcg ccaattagga tctggtttgg ctttgccatg ttttgtatct tggcagctat
     5161 gttcatcaca gggcctaaga tgtcaacgtg tgattttttt gcatcggcgc catatctgac
     5221 gatcatgttt tgaccataat caattccaat tttaatggca agatctggat agtcatattg
     5281 gttcagaatc ggattgattc ctttttggat gacagatagc atcgattttg cgcagaggac
     5341 agcgttatca gcaggttgca attggttttc ttctgcagga aagtatccaa tcaccgcgtc
     5401 gccaacaaac ttgagaacgt aaccattgtg atgatgtatg gttgctgcca tttcctgggc
     5461 aaaacaggtg atgattattg cgaccttatc ctcaggcagc tcaagtgtta tttgtgtaga
     5521 gcccacaaga tccacataca gaatcaccat cctcattctg gaaaagacgt gctttcttag
     5581 aaacgcatct gactcgtctt ctactggaga atattcatat ccacttttta gggcgcgcca
     5641 gactcggttt tgggtttcct tgatcagcgt atcagagtca actgcgcgct cacttggctt
     5701 gcttaacatc atatctatta tgtcataatc tgtttttggt ttcactagtg gtttttcttg
     5761 gggagtttcg ttaaccttag cgttgctatc tatgctgctc aagtaattat caccttattc
     5821 tagcggaaag ctaacatcac aaattgggca tttggcccgt tttcctttat aagcatcatc
     5881 atagtactgg actacttcga cattacagct taagcagatt atcttagtaa ccgacattca
     5941 catgtaatag gcgcggtttt ctatttaacc ctgatgaaga acaaatcaga cattcacgaa
     6001 tagaatagat tatgtcatga ttaccaggta ttcct
//
LOCUS       DASWKH010000063         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2023987, whole genome shotgun sequence.
ACCESSION   DASWKH010000063 DASWKH010000000
VERSION     DASWKH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023987"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGA92_04845"
     CDS             <1..197
                     /locus_tag="VGA92_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845774.1"
                     /translation="AEKNKLIIVNAQKKNFELACFASLDKNIIQPLQDQWKFIEVEKI
                     GRTRYVKITEAGSNAAEFLI"
     gene            complement(239..895)
                     /locus_tag="VGA92_04850"
     CDS             complement(239..895)
                     /locus_tag="VGA92_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845775.1"
                     /translation="MRFAELEFGSFLTYSPHGNSEAELLSKTAMSDLKNDKYITLNSK
                     QILMSDYLAKGIKMRLDTLPFAEYFKVDPILIPTPKSSLSKSNTIWVPQRLASALVKE
                     GLGQDVKSCLQREKPVAKSSMSLPENRPKAIEHYNSMAVQKTLDEPKEILLVDDVITR
                     GATLLGAANKLADAFPNARIRAFAFMRTISTSAEFENIFDPCKGKITLRDDGSTFRRP
                     "
     gene            complement(904..1569)
                     /locus_tag="VGA92_04855"
     CDS             complement(904..1569)
                     /locus_tag="VGA92_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX9845776.1"
                     /translation="MSVENLLGRTLNDIETKYAPELLYVQGPLEIPLPCPRVSVIGTR
                     KPSPEGLADAQSIAKTLTKNQIIIVSGLAEGIDTGAHRAAIDSGGRTIAVLGTPLNKV
                     YPQKNSELQQEIMEGHLAISQFPVEYPTTRKNFIIRNRTMALISDASIIVEAGDSSGS
                     LHQGWETLRLGRPLFIWKSLLSNSELDWPKTMIKYGAMELANPMDVLEVIPSHLKMID
                     LFQ"
BASE COUNT          592 a          396 c          416 g          693 t
ORIGIN      
        1 ttgcagaaaa aaacaagctc atcatcgtaa atgcacaaaa gaaaaatttt gagctggcat
       61 gttttgcaag tcttgataaa aatattatac agcctttgca agatcagtgg aagtttatcg
      121 aggtagaaaa gattggacga acacgttatg tgaagattac cgaagctggc tcaaatgcgg
      181 ctgagttttt aatttaggta tgtttcattt gcaaaaccta agagttacca attatttttt
      241 atggtcttct aaatgtgctg ccatcatctc taagagtaat ctttccttta catggatcga
      301 aaatattttc aaattcagct gaagtactta ttgttcgcat aaatgcaaag gcacgaattc
      361 tagcattggg aaaagcatct gcaagtttgt tagctgcgcc aagaagagta gcaccacgcg
      421 ttataacatc atccactaaa aggatctcct taggttcatc tagtgttttt tgaacagcca
      481 tagagttgta gtgttcgata gcttttggtc tgttttcagg caaactcatc gaagatttag
      541 caactggttt ttcacgttgc agacatgatt tgacgtcttg tccaagacct tccttaacta
      601 aagcacttgc aagacgttgt ggaacccaaa tagtatttga ttttgacaaa gaacttttgg
      661 gagtaggaat tagaatgggg tctactttaa agtattcagc aaaagggagt gtatcaagac
      721 gcatcttgat tcctttggca aggtaatctg acattagaat ttgttttgaa ttcagtgtga
      781 tgtatttgtc attcttaagg tccgacattg cagtcttgga gagtagttcg gcctctgaat
      841 tcccatgtgg tgaataggtt aagaaagaac caaattcaag ttcagcaaag cgcatgacaa
      901 aagttactgg aataaatcga tcattttaag atgcgatggt ataacttcca acacgtccat
      961 tgggttagca agctccattg ctccatattt tatcatagtt tttggccaat ccaattcaga
     1021 attactcaag agagatttcc aaataaacaa tggtcttcct agtctgagag tttcccagcc
     1081 ttgatgtaac gaaccgctcg agtccccggc ttcaacgatt attgatgcat cagagattaa
     1141 agccatggtt ctatttctaa ttatgaaatt ttttcgagtg gttggatatt ctactggaaa
     1201 ctgtgaaatt gcaagatggc cttccatgat ttcttgttga agctcagagt ttttctgtgg
     1261 atatactttg tttaagggag tcccaagtac tgcgatggtt cttcccccac tatcaatggc
     1321 tgctctgtgt gctccagtat caatcccctc tgcaagtcca ctgacgatta taatttgatt
     1381 tttggtaagg gtttttgcaa ttgattgtgc gtcagctagt ccttcaggcg agggctttct
     1441 tgtaccaatt actgagactc tgggacaagg taatggaatt tccagtggac cctgaacgta
     1501 aagcaattca ggggcgtatt tagtttcaat atcatttaga gttcgaccaa gaaggttttc
     1561 aacagacatg gaacaaaatt ttgaccccgt tagcatcctc atcacattca tttccagatt
     1621 attattttaa tacttttgag aggccttttt tgaccaaaac atggtaaccg atcacaatag
     1681 gtgtatcaat tccctatttg cgtggtttta actaagatta aatctgtgat tttctgctct
     1741 caaaatctca tatttaatac ccgaaattgt tggtttttaa tcctaaattg cggttgggat
     1801 ttcgcacttt tcacgctcaa cccacctccg gtacttttag ctaacgccga ttagctagtg
     1861 gcctaaccta gttgtgaatc tgttaagact agaaccgttt gcgttcaagt ttgcttgatc
     1921 actttattac cgcatagatt ccggtcctgt tgacaaaacg aatacctttt gagtaaagta
     1981 tgtcagaatc gccatccact tgagtgtaga tctggcctct tattttttta gcatattttt
     2041 ttgcccatac aagcgactgg tacagtctta gtttatggta ggcttctatt ccataac
//
LOCUS       DASWKH010000064        10257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_538639, whole genome shotgun sequence.
ACCESSION   DASWKH010000064 DASWKH010000000
VERSION     DASWKH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538639"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VGA92_04860"
     CDS             <1..1486
                     /locus_tag="VGA92_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9845777.1"
                     /translation="GVSGPVTCFPNAPISDFKDTVAIHLTGTFWTSAQALKVMKAGAK
                     IITISTFFTEERPYEQRPYRFRSPYTAAQGAKNRLAEAMSWELTERGIISIATNPGPV
                     HSDRIYKTVYPKAAAEFLRVSGFEDLAPSEVEAANKEIIGLLGESEQTVKDGIAKAAN
                     IIAKMKNADVEKTAQTLSRLLSKIQEIAEKVQKNTSFMIADRQFLSQQQVAQTVMMLA
                     DDNIAKILNGKVIPGDRVFYPVKPHITTSTPTVQQPNFSSQVVVLAIDATDEADIGRA
                     EFLAQHVEKNGGKAVCLISEKTPKEFKDRLISKFHAHTVNLESPDEVKRWLEATKNLG
                     KFTILVYLTGKVPANLKIIELTRKNWDGLVDKFINTPATVIQSALDFFVAGGKKDPRL
                     YKNATGTVMVVGPDMPLGAKTTGVDRARVEVFRGALRPFTTTVNQELSDVLKSKIRSF
                     LVLPGSIDGKEPDNAKIAGALNYFVTDSARESSEVTFYVDETRT"
     gene            1487..1933
                     /locus_tag="VGA92_04865"
     CDS             1487..1933
                     /locus_tag="VGA92_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845778.1"
                     /translation="MKKFADLKVGDEFFSTCTFSKKELETYLAFSRIKNAIFDDDEYS
                     TIVSGRAVIARMEGEFTRLSQIYGNMILLYGMNGDPNWENRNARFLKPLHTDEILKIK
                     FAISDKKDLDNEFGMIAVDFEGKKENGDLVVVAKKNLYRIKREPPK"
     gene            complement(1964..2116)
                     /locus_tag="VGA92_04870"
     CDS             complement(1964..2116)
                     /locus_tag="VGA92_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845779.1"
                     /translation="MVKFDGIRMQELIEVQDPDKDGGITLVFQDNRYLRIRVNDGKLE
                     TVSEPE"
     gene            complement(2165..2917)
                     /locus_tag="VGA92_04875"
     CDS             complement(2165..2917)
                     /locus_tag="VGA92_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9845780.1"
                     /translation="MFENLWLIPLGFIAGVVGSIIGLGGGVIVVPVLTFLGVPPTVAA
                     SSSLFAAFSNSVASTVSYAKQKRIDYNIGLKLGLMSIPGTILGAFATAEVTPSIFKIL
                     FGIVLVASCYYLFIKRDMESKQKTITKRMFVLSAGISFFAGILSSFFGIGGGIVFVPL
                     MIIGLGLLVKTATATSQLILMFSSASGMIAHFLLGHSDFDYAFLLAVGAFVGGLVGAR
                     LSVELKENSIRILICAVIVAAAIKLFIDGFSL"
     gene            complement(2960..3859)
                     /locus_tag="VGA92_04880"
     CDS             complement(2960..3859)
                     /locus_tag="VGA92_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9845781.1"
                     /translation="MAAINPAINFGQSLSEKTLLDKIKRLESEKQRLWEKNTLQQKTL
                     ITLERKLAKFDEIKEQRDWLLDEMTKKIGELNRFQSEFFKAEKFTVIGEMAARLAHDM
                     RNPLAIIKNTVELVKLKHSSKIPAELLHLFVSIDNASSRLVFQLDEVLNFVRRSPLEY
                     DEYSLHDILESAMNKIIIPDEIRIHKPANNVCLFCDRQKIEAVFTNLLMNAIQAMENS
                     GDVIIRFSEGDEVVVFTVQDSGPGIPANMIDKIFEPLFTTTSRGTGLGLPAVKTIIQQ
                     HQGTINVTNGPTVFSVSLPKGPL"
     gene            complement(3920..4543)
                     /locus_tag="VGA92_04885"
     CDS             complement(3920..4543)
                     /locus_tag="VGA92_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845782.1"
                     /translation="MTNKKILYLAMGVAVASIISVLAIINNNDGNIQKFSGYDDANKI
                     LKQELLEYDISMSSPIKLQRQEDVQKYCTFFTSEEQQNLVQYCTSTELKDNDGKFLGN
                     VHMVGSSDEPKIILVLVQVDSVMSQIDSVKNIVDTTIQNLVCDCWNQVRPDGFEDVGQ
                     WVDGLRQFHLQDTKPHSKSKEITLSGQVLQLELTTNKDGYLWQFFVY"
     gene            complement(4586..5080)
                     /locus_tag="VGA92_04890"
     CDS             complement(4586..5080)
                     /locus_tag="VGA92_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX9845783.1"
                     /translation="MATRAQILIPIGIAAVIIGVVGMMSIPKDVKLEQAEFPMGIIKI
                     DDVTLQVQIADTKPLQTRGLMFQEKLPYDQGMLFIFEGDDVRSMWMLNMQFSLDLIWI
                     DSDGKVVHIEKDAQPCKSALETMACTFTNGNGKPAKYVLEVTSGFVDMFNITENSQLQ
                     LISI"
     gene            complement(5121..5429)
                     /locus_tag="VGA92_04895"
     CDS             complement(5121..5429)
                     /locus_tag="VGA92_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845784.1"
                     /translation="MSDSVKRRAKLYSKISEIDDKGAAKSMTALRKASDPGGKIQKIG
                     FIVFWIPEPTGISNAVGAPMILAGKFLEKKYNGATLNDISKGTKNNLQTISHFKDSVF
                     "
     gene            5940..6335
                     /locus_tag="VGA92_04900"
     CDS             5940..6335
                     /locus_tag="VGA92_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HEX9845785.1"
                     /translation="MARIEDYLEVIAELVEQKGYATTLDVSRYMNVSAPSVTKMLQRL
                     DENGYLKYEKYHGINLTPKGNNLADAIRQKHGILLEFFEILGIEHETANQDAEGIEHH
                     LNPKTIKQLRKFITYLKSNQKFLEGFKTP"
     gene            complement(6322..8148)
                     /locus_tag="VGA92_04905"
     CDS             complement(6322..8148)
                     /locus_tag="VGA92_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HEX9845786.1"
                     /translation="MSKIVADTSVIISGYITKQIESKALQNFELIIPIAALDELQSQA
                     SQGKEQGFVGLEEIKKIKKLCQESGIMMQFVGQRPSLDDIRLARHGRIDAIIKDIAKQ
                     NNAILYTADYVQGLTAEAEGITVSFQKSEKPPTSLEFLRFFDQNTMSVHLKEGTQPFA
                     KRGRPGSFQLLKLEDNILEKQYLELITTQILEASKISNAGTIEISKPGALIVQYGDYR
                     IAITRPPFSEAHEITIVHPIIKMSLDQYHVSDKLMKRFSEQAEGVIISGPPGSGKSTL
                     ASSLANFYAEKGNVVKTFESPRDLQVNKEVTQYTKLDGSFENSADILLLVRPDYTIFD
                     EMRQLHDFRVYADLRLAGVGMVGVVHANMPLDAIQRFIGKIDLGMIPSVIDTVVFVKD
                     GSISKVYDLELKVKVPSGMVEQDLARPVIEIRDFETGVLEYEIYTFGEENVIVPVSVS
                     EAKSGIQKLAAEKIKEIIRRFDPNPEIEILSENSIKVKVKKDSIPSIIGRGGSTINDL
                     EKILHVHIDVEPKSTGESDYSEPTQNVQFDFSESKNSLLFAVDKRCSGMFAEIYVRGQ
                     FLTAARVARHGKIKISKRSDAGKSLMQSAFTKKDIEIFLRES"
     gene            8239..9159
                     /locus_tag="VGA92_04910"
     CDS             8239..9159
                     /locus_tag="VGA92_04910"
                     /inference="COORDINATES: protein motif:HMM:NF037270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845787.1"
                     /translation="MSDIEDKDVIIIEDSPAVGILLKEFLNKLGLKKIHHCQNGKTGI
                     ESFKEIVESGGNPLVFLDYNLPDMTAFSIMTQMLSIRPDVKVIIETAREKSEDSIKDV
                     IAQGAYQYLGKPIRLEKLKEIIDTLRLEEAPPVDEGASKILEKIVSSATQISILRISE
                     HLNKKNSEILPQIQQLISDKKIVQINDIREVGCPRCSSVRVTQTFHCPKCKGTNFKQD
                     KIIEHYKCGNVSSASSYADDKCPKCHQPIKVFGVDYRTQDNMFTCNDCAEIFSEILTN
                     YLCLKCNDKFTLEHAKFLTSTGYSWLKAVT"
     gene            complement(9152..9511)
                     /locus_tag="VGA92_04915"
     CDS             complement(9152..9511)
                     /locus_tag="VGA92_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9845788.1"
                     /translation="MARIMVADDSDAIRLVLQDILSIGKHEIVAEATNGAEAVERFSQ
                     ANPDVLLLDLAMPKKDGLTVVKEVMEKHPNAKIILITASDNQNIINECLRAGASAFIS
                     KPFDFDYVLKLIEETLK"
     gene            complement(9552..9914)
                     /locus_tag="VGA92_04920"
     CDS             complement(9552..9914)
                     /locus_tag="VGA92_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX9845789.1"
                     /translation="MSDEFLRVAKKEVSEDIAEIGNLLKNCQNDNDVLQNAAGIEKRI
                     HKLKGLAPMMGHEQIGEIAALVDKLLKVAMSGKVVLGIYDTIKNSHQFMHNALNDVDA
                     NFVSLKGQIEKNHDSFLH"
     gene            9973..>10257
                     /locus_tag="VGA92_04925"
     CDS             9973..>10257
                     /locus_tag="VGA92_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX9845790.1"
                     /translation="MKIISKTYLLIGVLIIVAVINLFVLYTTQSTTNNESYSIINAGD
                     LKTKVETIASLASSIATGNENDRLRLEKEIKEFDGVLVALRNGGIIRGQPT"
BASE COUNT         3026 a         2108 c         2020 g         3103 t
ORIGIN      
        1 tggagtttca ggtcccgtca cctgttttcc aaatgcgcca ataagcgact ttaaggacac
       61 agttgcaatt caccttactg gcactttttg gacatctgca caggcactga aagtgatgaa
      121 ggccggtgca aaaatcataa caatttccac attctttact gaagagcgac catacgagca
      181 aaggccgtac agattccgaa gtccctacac tgcagcacaa ggtgcaaaaa atagactcgc
      241 agaggcaatg tcttgggagc taaccgaaag aggaatcata tccattgcta caaacccggg
      301 cccggttcac tctgacagaa tatataaaac tgtttaccca aaagcagctg cggaattttt
      361 gcgcgtaagc ggctttgaag acctagcacc gtcagaagtt gaagctgcaa acaaggaaat
      421 tattggatta cttggcgaaa gcgagcagac tgtaaaagat ggaattgcca aggccgcaaa
      481 catcattgca aaaatgaaaa atgccgatgt agaaaaaact gctcagacat taagtaggct
      541 gttatcaaag atacaagaga ttgcagaaaa agttcaaaaa aacacctcat ttatgattgc
      601 ggacagacag tttctctccc agcagcaggt tgctcaaact gtcatgatgc ttgcggatga
      661 caatattgca aaaatactaa atggcaaggt gattccaggt gacagggttt tctatccagt
      721 aaaaccccac ataaccacat ccacgccaac cgttcaacag cctaattttt catcacaggt
      781 tgttgttctg gcaatagacg ccacagatga ggcagacatc ggcagggctg aatttcttgc
      841 ccagcacgta gaaaaaaatg gcggcaaagc agtttgcctc atctcagaga aaacaccaaa
      901 ggaattcaaa gacagattga tctcaaagtt ccacgcacat acggtgaatt tggaaagtcc
      961 tgacgaggtg aaaagatggc tggaagcgac aaaaaaccta ggcaaattca ccattcttgt
     1021 ttatttgaca ggcaaggtac cagcgaatct gaaaatcatc gaactgacaa gaaagaattg
     1081 ggatggcctt gttgacaaat tcatcaacac gccagctact gtaatccagt cagctcttga
     1141 tttctttgtt gcaggaggta aaaaagatcc gcgcctttac aaaaatgcca cagggacagt
     1201 aatggtagtt ggtccagaca tgcctcttgg cgcaaaaaca acaggagtcg acagggcacg
     1261 agtcgaagtc ttcaggggcg cactcaggcc atttacgacc actgtaaatc aggagctaag
     1321 tgacgtgctc aaatcaaaga ttagatcatt tcttgtcctg cccggaagca tagacggcaa
     1381 agagccagac aatgccaaga tcgcaggagc tttgaattac tttgtgactg attctgcaag
     1441 agaatcttcc gaagttacat tttatgttga tgagacaaga acataaatga aaaaatttgc
     1501 agacctcaaa gtaggtgacg agtttttttc cacgtgcact ttctcaaaaa aagagcttga
     1561 gacatatctt gcattttcaa gaatcaagaa cgcgattttt gatgatgatg agtacagtac
     1621 catagtgtct ggccgtgccg ttattgcaag aatggagggc gaattcacaa gactcagcca
     1681 gatttacggc aacatgattt tgttgtatgg gatgaatgga gacccaaact gggagaacag
     1741 aaacgcgcgt tttctcaagc cgctacacac agatgaaata ctcaagataa aattcgcaat
     1801 atcggataaa aaagatcttg ataacgaatt tggaatgatt gctgttgatt ttgagggcaa
     1861 aaaagaaaac ggggatcttg tggtagtagc aaagaaaaat ctttaccgta taaaaagaga
     1921 gcctccaaag tgatttttca gggcatctag gcgtaaaaac gagttattct ggctcagata
     1981 ccgtttcaag tttgccatca tttactctga ttcgcaaata cctattgtcc tgaaacacca
     2041 gtgttattcc accgtctttg tcaggatcct gtacttcaat tagttcctgc attcttattc
     2101 catcaaattt taccatgttt tttgcacacc gtgccagata ttatatttaa tcatctgtag
     2161 taagttaaag gctaaatcca tctatgaata atttgattgc agctgccact attactgcac
     2221 aaataagaat ccggatgctg ttttctttga gctcaacaga cagtcttgcc cctaccaagc
     2281 cgcctacaaa tgccccaaca gcgagcaaaa acgcataatc aaaatcagaa tgaccaagca
     2341 agaaatgagc tatcattcca gatgcggatg aaaacatcag gattagctga gatgtagcgg
     2401 ttgctgtctt taccaataat ccaagtccta ttatcatcaa tggcacaaaa acaattccac
     2461 cacctattcc aaagaagcta gaaagtattc cagcaaagaa gcttattcct gcagagagta
     2521 caaacattcg ttttgtaatg gttttttgtt ttgactccat gtctcgtttt atgaaaagat
     2581 aataacatga agcaaccaga acaattccaa acaaaatttt gaatattgat ggggttacct
     2641 ctgctgtagc aaaagcacca agaatcgtac ctggaattga catcaatcct agctttaatc
     2701 caatgttgta gtcaatgcgt ttttgttttg cgtaagaaac agttgatgcg acagaattac
     2761 taaatgccgc aaagaggctg ctgctggcag ctacagtagg cggaactccc aaaaatgtca
     2821 acacaggaac tacgattaca ccacctccaa gaccaattat agagcccact acaccagcta
     2881 tgaacccaag cggaattagc cacagatttt caaacatttt tgctataccg aataattagc
     2941 tattgaatct tgtcgttttt tagagtggtc cctttgggag agaaactgaa aatactgttg
     3001 gtccgtttgt tacatttatt gttccctgat gttgttgaat tattgttttg actgcaggca
     3061 acccaagccc agttccacgt gacgtggttg taaataacgg ttcaaatatt ttatcaatca
     3121 tatttgctgg aattccaggc cccgaatcct gaactgtaaa gacgacaact tcatcacctt
     3181 cagaaaatcg tattattaca tcaccggaat tttccattgc ttggattgca ttcattagta
     3241 gatttgtaaa tactgcttca attttttgcc tatcacaaaa aaggcagaca ttgtttgcag
     3301 gtttgtgaat cctgatctca tcaggaataa ttattttgtt catggcggat tcgagtatgt
     3361 cgtgtaaaga gtattcatca tactcaagtg gtgatcgcct aacaaagttt agaacctcat
     3421 cgagctgaaa taccagtctt gatgatgcgt tatctattga gacaaagaga tgaagcaact
     3481 ctgctggaat ttttgaagaa tgctttagtt ttacaagctc cactgtgttt ttgattatgg
     3541 ccaatggatt acgcatatca tgagcaagcc ttgctgccat ttctccaatt acagtgaatt
     3601 tttctgcttt gaaaaattct gattgaaaac ggtttaattc gccaattttt tttgtcatct
     3661 catctaggag ccaatcacgc tgttctttta tctcatcaaa ttttgcaagc tttcgctcaa
     3721 gggtaatgag tgttttttgt tgcaatgtat ttttttccca gagtctttgc ttttcagatt
     3781 caagtctttt aattttgtcc aaaagcgttt tttccgacag agattgccca aaattaattg
     3841 caggatttat tgctgccaag acccgaggag ttttgcctag aattagataa taatgggctg
     3901 tttgttcaaa acagacaaac tagtatacga aaaactgcca caaatagccg tctttgttag
     3961 ttgtaagttc taactgtaga acttggccac taagtgtaat ttcctttgat ttgctatgtg
     4021 gttttgtgtc ctgcaagtgg aactgcctta ggccgtctac ccattgtcct acgtcttcaa
     4081 agccgtcagg tcgcacttga ttccagcagt cacacactag gttttgaatg gtcgtatcaa
     4141 ctatgttttt cacagagtca atttgggaca taaccgaatc tacttggact aatacgagaa
     4201 tgatttttgg ttcatcagaa gagccaacca tatgcacatt gccaagaaac ttgccgtcat
     4261 tgtcttttag ttcagttgat gtgcaatatt gtacaagatt ttgttgttct tcacttgtga
     4321 aaaacgtaca gtatttttgc acgtcttctt gtctttgtag ttttatgggt gaagacattg
     4381 aaatatcata ttccaataat tcttgtttta gtattttgtt tgcgtcatca tatccagaaa
     4441 acttttgaat gtttccgtca ttgttgttaa ttattgccaa tacagaaatg attgaggcta
     4501 ctgctacgcc cattgcaaga taaaggattt ttttgtttgt catttttttg ttgcacttag
     4561 ttactccaga taagtctttt caagattaaa ttgagattaa ttgcagttgt gaattttctg
     4621 ttatgttaaa catgtctaca aaaccagaag ttacctccag tacgtatttt gctggcttgc
     4681 cgtttccgtt tgtgaacgtg catgccattg tttccaacgc ggacttgcaa ggttgtgcgt
     4741 ctttttcaat gtggactact ttgccatccg aatcaatcca gatcaaatcc agtgaaaatt
     4801 gcatgtttag catccacata gaacgcacat catcaccctc aaaaataaac agcatgcctt
     4861 ggtcatatgg cagcttttct tggaacatca accccctcgt ttgcaggggt ttggtatcag
     4921 ctatttgtac ctgcaaagtc acatcatcta tttttattat gcccattggg aattctgcct
     4981 gttcaagctt tacgtctttt ggaatcgaca tcattcccac cacacctata attactgcgg
     5041 caatgccaat cggaattagg atctgagcgc gcgttgccat gtaattaaga aattatctgg
     5101 ttttaaaaac taaatgcgca ctagaacaca gaatctttga aatgagagat tgtttgcaag
     5161 ttgttttttg tgcccttact tatgtcattt agggtcgcgc cattgtattt tttctcaagg
     5221 aacttgcctg caagtatcat tggtgctcca acggcattag atatgccagt aggttctgga
     5281 atccaaaata ctatgaagcc tattttttgt atttttcctc ctgggtctga tgccttccgc
     5341 agcgcagtca ttgatttagc tgcacctttg tcgtcaattt cggaaatttt agaataaagt
     5401 tttgctcgcc tctttacaga atccgacatt agtttcaact tgcgcagtcg gtgctcatac
     5461 ctgaccatga caccgtcaat ttaggcataa ttagttaagc gcaaaaccaa aagatcaatt
     5521 actagaaggt caagatcatg tgaactacca ctcaataagt tgagtggccg cttcctgctt
     5581 caacgaccga gattctgatc tccgcaggcg tgcattcccg tagttcctac ggtagtctgt
     5641 ccggcccaat cctgttttgg ctttacatga cgtcaggtac aggcctgtat cgtggtcacg
     5701 accttcttcc attttgccta gctctgacat tggatatttt tttgaatcat tacatttaac
     5761 atgctggtta ttatgtaatt accagatatt caatatcggc gattcatcta ctggttaaaa
     5821 cgatggtctt ctagcctagt ttttataagt cgcgtttggg ttaagaaacc aatttgaaaa
     5881 aaaagggtga aagtagactc gaatccatca aggccgcaca caagtcagcc aagccgaaga
     5941 tggcgcgaat cgaagactat cttgaagtca tagctgaact agtagaacag aaaggatatg
     6001 ccaccacgct agatgtgtca agatacatga atgttagtgc tccaagtgtg acaaagatgc
     6061 tgcaaaggct tgatgagaat ggctatctca aatatgaaaa atatcatgga attaatctta
     6121 cgccaaaggg caacaacctt gcagatgcga ttcgacaaaa acacggcata ctcttagagt
     6181 tttttgaaat actgggaata gagcacgaga ctgcaaacca agatgccgaa ggaatagagc
     6241 accatcttaa ccccaagacg atcaagcagc ttagaaaatt tatcacttat ttgaaatcaa
     6301 atcaaaagtt tcttgaggga tttaagactc cctgagaaat atttcaatgt ctttttttgt
     6361 aaaggccgac tgcatcaggc tcttgcctgc atcagaacgt tttgaaattt ttatcttacc
     6421 atgtcttgca accctcgcag cagtaagaaa ttgtcccctg acatagattt ccgcaaacat
     6481 tccgctgcat cttttatcta ctgcaaataa aagcgagttt tttgactcgg aaaaatcaaa
     6541 ttgcacgttt tgtgtaggtt ctgaataatc tgattcgccg gtgctttttg gctctacgtc
     6601 aatgtggacg tgcagtattt tttcaagatc gttaattgtt gagccacccc ttccaataat
     6661 agatgggatc gagtcttttt tgactttgac tttgatgctg ttctcagata aaatttcaat
     6721 ttctggattt gggtcaaacc gtcgtattat ctccttaatt ttttctgcag ctaatttctg
     6781 aatccctgac tttgcttcgg aaactgaaac tggaacgatt acgttttctt caccaaatgt
     6841 gtatatctca tattccaaga cgcctgtttc aaagtcacgg atttcaatta ctggtcttgc
     6901 taggtcttgc tctaccattc ctgacgggac tttgactttg agctccaaat cataaacttt
     6961 ggaaattgag ccgtccttta cgaacaccac tgtgtcaatt acgcttggaa tcatgcccag
     7021 atctattttg ccgatgaacc gttgtattgc atcaagtggc atgtttgcat gaaccacacc
     7081 aaccatccca acccccgcaa gcctcaagtc tgcataaact ctaaagtcat gtagctgcct
     7141 catctcatca aatattgtgt aatctggcct cactagtaac aaaatgtctg ccgagttttc
     7201 aaaactacca tcaagctttg tgtattgtgt cacttctttg tttacctgaa ggtcccgtgg
     7261 agattcgaat gtttttacga cgtttccttt ttctgcgtag aaatttgcaa ggcttgaggc
     7321 aagagtactt tttccagatc ccggaggccc cgaaataatt actccctcag cttgctcaga
     7381 gaaccttttc attaatttgt ctgaaacgtg gtactgatca aggctcattt ttatgatcgg
     7441 gtgaacgatt gttatttcat gcgcttctga aaatggcggc ctagtaatgg cgattcggta
     7501 gtccccgtat tgcactatca atgcgcccgg ttttgagatt tcaattgtac ctgcgtttga
     7561 gatttttgat gcctctaaaa tttgcgttgt aataagctca aggtattgct tttctagtat
     7621 attgtcttct agtttgagaa gctgaaatga gccgggcctt cccctttttg caaagggttg
     7681 ggttccttct tttaggtgca cactcatagt attttgatca aaaaacctca gaaattccag
     7741 gcttgttggg ggcttttctg atttctgaaa agacactgta atgccttctg cttctgcggt
     7801 aaggccctgc acataatcgg cagtatagag aatagcgttg ttttgctttg caatgtcttt
     7861 gattattgcg tctattcttc catggcgtgc aagccgtatg tcatcaaggc tcggcctttg
     7921 gcctacaaac tgcatcatta tgccactttc ttggcagagt tttttgattt tttttatctc
     7981 ttcgaggccc acaaatcctt gttcttttcc ctgcgatgcc tgagactgta attcgtctag
     8041 ggcggcaatt ggtatgatta gctcaaaatt ttgaagtgct tttgattcta tttgttttgt
     8101 aatataaccg ctaataatca cgctagtgtc tgccactatt tttgacatga ttacaaggtt
     8161 actgtgggac ttttaatcat aactcaatac tacagatgca aactaaactt ttcatagtag
     8221 gtacaaagat gaaaaagtgt gtcagacata gaggacaaag atgtgatcat tattgaagac
     8281 agtcctgcag tagggatttt gctcaaagaa ttcctcaata agcttggtct gaaaaaaatc
     8341 caccactgcc aaaatgggaa gactggcata gaatccttta aggagattgt agagtcaggc
     8401 ggaaatccac tggtgtttct tgattataat ttgcccgata tgactgcatt ttccattatg
     8461 acccagatgt taagcatacg accagacgtc aaagtaataa ttgaaactgc tcgggaaaaa
     8521 agcgaagatt cgatcaaaga tgtgattgca cagggcgcat accaatatct aggaaagcca
     8581 ataaggctag aaaagcttaa agaaattata gatactcttc gtcttgaaga ggcaccgcca
     8641 gtagatgaag gagcaagtaa aatacttgaa aaaatagtgt cgagtgcaac tcaaatcagc
     8701 attttgcgaa tctcagaaca tttgaataaa aaaaattctg aaatcctccc acaaatacag
     8761 caattaattt ctgataaaaa aatagtgcag ataaacgaca tacgtgaagt aggctgcccg
     8821 aggtgtagtt ctgttcgcgt tactcagaca tttcattgtc caaaatgcaa gggaaccaac
     8881 tttaagcagg acaaaataat tgagcactac aagtgtggca acgtatcttc ggcatcctca
     8941 tacgcagatg ataaatgccc aaagtgccac cagccaatca aagtgtttgg cgtcgactat
     9001 agaacacagg acaacatgtt tacctgcaac gactgcgctg aaatcttttc agaaattttg
     9061 acaaattatc tgtgtttgaa atgcaatgac aaatttactt tggagcatgc caagtttttg
     9121 accagtaccg gctacagctg gttaaaggca gttacttgag tgtctcttca atcagcttca
     9181 acacataatc aaaatcaaat ggttttgaaa taaaagcaga cgccccggcc ctaaggcatt
     9241 cattaattat gttttggttg tcgcttgcag tgataagaat tatttttgca tttgggtgtt
     9301 tttccattac ttcttttaca accgtaagcc catctttttt tggcattgca agatcaagta
     9361 gcaaaacatc cggatttgct tgggaaaatc tttcaactgc ctctgcacca tttgtcgcct
     9421 ctgctacaat ctcatgtttt ccaatagaga gaatatcctg aagcaccaga cgaatcgcat
     9481 cagagtcatc tgcgaccatt attcgtgcca tatcaacaaa tcaattacgg catatcttat
     9541 gaaaatttcg attagtgaag aaatgagtca tgattttttt ctatttggcc ttttaatgag
     9601 acaaaatttg catccacatc attcaaagcg ttgtgcatga actgatgaga gttttttatt
     9661 gtgtcataaa tgcccaaaac cacctttcct gacattgcga ctttgagaag tttatcaact
     9721 agtgccgcga tttcccctat ttgctcatgc cccatcattg gcgcaagacc ctttagtttg
     9781 tgtatgcgtt tttcaatccc tgccgcattt tgtaaaacat cattgtcatt ttgacaattt
     9841 tttagcagat tgccaatttc ggcaatatct tcagatacct ccttttttgc cacccgtaag
     9901 aattcatcag acatttacta acattatttt gatcgagatt tttatactct atcgaaggaa
     9961 ggttagctaa aattgaagat tattagtaag acgtatctac ttatcggggt tttgatcata
    10021 gttgcagtca ttaacctgtt tgtgctgtac accacacagt ccactaccaa taacgaatca
    10081 tattccatca ttaatgcagg cgaccttaag actaaagtcg agacgattgc aagcctggca
    10141 agctcgattg caacaggtaa tgaaaacgac aggctgagac tggaaaagga aatcaaagag
    10201 tttgacgggg tgctagttgc cttaagaaat ggtggaatta tcagggggca gccaact
//
LOCUS       DASWKH010000065         8798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1654327, whole genome shotgun sequence.
ACCESSION   DASWKH010000065 DASWKH010000000
VERSION     DASWKH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654327"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="VGA92_04930"
     CDS             complement(<1..2231)
                     /locus_tag="VGA92_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX9845791.1"
                     /translation="MTNFGTLEYVMDKFSGTWSWKITGERAVAMVSRIIPQAWYGDHE
                     FEAIVPDDPKNLQQIKWIMDRYPLEILSKNVWQRKIPPSFKHKPKKPKRIEKLRLANP
                     GKQFKGTLFNFQREGLDFLIKSSGNALLADEMGLGKCTVGTSLIETINGQVHIGDLWN
                     GSSVIEQNGDETWAKLSKPITIQTFDGQKITAKKITRIFRQKISEPIVKLTLWDGSVI
                     ECTQRHRFLTPQGWQMAGELTVDDKVAVPAKTLHSGKNNLEPDLAELMAWQLAEGHEF
                     YTKEGNAVGIFRNSDPIVLSRVHELARKYGIVLPIAKRNGRGFVISTTRLHSLFTRFG
                     YQWGKKSADKIIPKSVLESSLEAQKAFVHAMFDAEGHVGKTHCEFTTASRNAAYGMRD
                     ILRHFGIWLRIRVAMKCATNGAKIKRPYYVCTFGGNSVERFVTQFKLLTKRKQIALEL
                     LAKKQHNTNVEGIPSQKIVQKIANTEISLRSLKLGTPYKYTQGMSKDLAYLVIDKLES
                     TNHSRLASELQKLALDAIHWCGIKEIQTKNYAGYVYDLSVPETHNYIVNGMITHNTIE
                     TLAYLSTEQNAFPVLIVAPLVTLQNWQREIEKFLKRKSRNGRLLDGTPPNSTIIRVGK
                     SEDLGKYDFYIINYELLYKRYMDLAKLNLRTIVCDEVQHLRSKTTQKYHAIKKLAALE
                     SIKYRIGLSGTPIYNRGSEIWPIVDILRPGLLGNFREFCEYFCYINEKGKAIVLENKR
                     ESLR"
     gene            2461..3159
                     /locus_tag="VGA92_04935"
     CDS             2461..3159
                     /locus_tag="VGA92_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9845792.1"
                     /translation="MKFTQSSEPVVEKPLLVAALQDMGNVGNIVIDFINKAKKSTPFR
                     TAESTQLSYVLDKGGHIEIPKEDWEYRYTKNMIVFGGGYGQPKTNEELHALCQDVISV
                     AKKYTAKFIYTVGGFHTEHPIEGEPKTFVTTTSEDLSKQLEKIGVSMTPSKSVITGFN
                     GMILGYAKLNDISGIGLYGELNEPAVPQYRTAKSVIKTLEKLTYQSFGETQELDMLAS
                     DIERKLRSGWRAEF"
     gene            complement(3323..3982)
                     /locus_tag="VGA92_04940"
     CDS             complement(3323..3982)
                     /locus_tag="VGA92_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845793.1"
                     /translation="MDLEKFRDDVYVATDKLSTQLQKDDIPKLNYVRQRLIELYEQNL
                     VKINHSVLELICAANLVSRGYAVDVEKQLTDSLVCDVYAKKDEHTTIIEIETGFTPPE
                     HALDTIDYYVSRIVSKIARYSKFCNEFSLATPVIGILPFHRIFLNPPSLRPEAEIKKT
                     KTGCDRFYRNPPIEYDDILNAKLDSIYLINIDKGFAKEIDPVSYYKLTASLLEKSEID
                     L"
     gene            complement(4077..6215)
                     /locus_tag="VGA92_04945"
     CDS             complement(4077..6215)
                     /locus_tag="VGA92_04945"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX9845794.1"
                     /translation="MLQLKFYTLLLTLIVLSSLSPSALGATSYTPAQNIVKSGDFVTL
                     QGEGVDPDDDSLTIMWRQTGGQQVTLMPSTGVAEPTFQAPEVENGKVIILTFELTVKD
                     PYGGVDTDIIKITVMPRNQPPTANAGSDQTVEKGDEVTLRGSGTDPDGDPLTYRWSQI
                     DGPIVDLDDPNSSSPTLDTSAMTRSSGTLRYQLTVTDGFGGLARDTVIIKVTAGKPTL
                     ISANAGTDKTVNEGQTVQLDGICTDKLNRDLSYTWTQTLGPFADLSSTADADPTFVAP
                     EIPNGAIVVLAFRFTCDAAEGGGTATDIVLIRVRPINDDPTADAGPDKETMSFRILYL
                     SGSGSDPDGDRLQYSWKQVSGTDVDVIYPNKADARFLTPEVFGGASTTLTFELTVTDP
                     YGGEDSDQVVITVKSDNVRASADAGADQVVDEHTEVTLAGTGEDPDSDEITFEWRQVA
                     GEPVELSATDAAEPTFVAPTVENGKTKVLVFELRVSDESGHPAKDTTRVTVLPVNAAP
                     VADAGDGQTVDIGITTTLSGSASDEDDDPLTYLWSQIFGPSVTLSAETDLTTDFVAPS
                     VEVDTTFTFQLIANDGQADSEPSTVDVVVKGLISKKLTAYAGSDQNVDEESVVTLFGS
                     GKDPMKHKLFYNWRQLSGEEVSLSASDLAKPSFVAPQVANGETKSLVFELMVSDGTGR
                     TAQDTVTITVGPINGDPTAIAKVKSVREPV"
     gene            6517..7422
                     /locus_tag="VGA92_04950"
     CDS             6517..7422
                     /locus_tag="VGA92_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX9845795.1"
                     /translation="MLLAVILLGNATIASAATPWDLIVRANLEQAEVSLNGKPVVFGT
                     VLDQAARPVSGVEVKISFSDRSATTTTDSEGNFRYEFGEQYMPGTFSVNVFAKLADKK
                     GFAKLTLKIGNESTTFSEIYYSSDKFAGSTTALPNDPYAAIKLKHLKEFIEEQNRIKQ
                     KQIDVEAKKLALHGKRSLAQQMLEKALRERPVGAGVFSDAQYERYLATVDPRIKDQIS
                     YQLNYTKQLRVEAQHAMKAVLDNGGSLEDARKAYFEKLSVTKEELLDVNDLNNTKNYS
                     KIKTNDDKKINSKKVKGLTVNKNLK"
     gene            7520..7756
                     /locus_tag="VGA92_04955"
     CDS             7520..7756
                     /locus_tag="VGA92_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845796.1"
                     /translation="MSTSISLGKGIGKQLSSILIDEEGVSLENIWETIADFGINRDDL
                     KKLQPTYKELFEIYSAIKAYRISVKYLKELKNKF"
     gene            7795..8394
                     /locus_tag="VGA92_04960"
     CDS             7795..8394
                     /locus_tag="VGA92_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845797.1"
                     /translation="MDINDLKEKLVLLSITAVVFLPLRLLVSQYISDHWLGNLGIATL
                     ISVSLIVLVRKNKLGRFGHVFKNQITKALWGRSAKLIIAALVFFMIYFGTTVLLIDRG
                     NTIYLDDKEILSQSIASRNLDRTALLSISGPQIHSIIGLAQIQYLEYLFSVSYAILND
                     TTNGWLVNLHLILFMEQVEVLGLFWFYKRMFKPIPTDAT"
     gene            8411..>8798
                     /locus_tag="VGA92_04965"
     CDS             8411..>8798
                     /locus_tag="VGA92_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845798.1"
                     /translation="MDRQIKNIILVLSRLGDNLYKALAVFSFVLILLYSNYVYATGDD
                     LEIKVLKGSSDEDLQIKSFYPEILPIAPNDAITWVNDDSATHSITSGISGHPDYSGKF
                     FKTGNIAPSKSSTVKIADTSSFAYYYF"
BASE COUNT         2473 a         1797 c         1810 g         2718 t
ORIGIN      
        1 cgcaatgatt cgcgcttgtt ttctaagaca attgccttgc ctttttcgtt tatgtaacag
       61 aaatattcgc aaaattccct aaagttccca agcagtcctg gtcttaggat atcaacgatt
      121 ggccaaattt ccgatcctcg attgtaaatt ggggttcctg agaggccgat tctgtatttt
      181 atcgattcaa gtgctgctag tttttttatg gcatggtatt tttgcgtcgt ctttgagcgg
      241 agatgctgca cttcatcgca cactattgtt cttaggttca gctttgcaag gtccatgtat
      301 cttttgtata acaactcgta gttaattatg taaaaatcat acttgccaag atcttctgac
      361 ttgcctacgc gaattattgt agagtttggt ggtgtgccat caagtagtct tccgtttctg
      421 ctttttcgct ttaggaattt ttcaatttcc ctttgccagt tttgaagtgt aacaagcgga
      481 gctacaatca aaactggaaa tgcgttttgt tcagtggaca gataagcgag tgtttctata
      541 gtgttgtgcg taatcatacc attaacaatg taattatggg tttcaggaac agacagatca
      601 taaacataac ctgcgtaatt ctttgtttgg atttccttta taccacacca atgaattgca
      661 tctaacgcta atttttgtaa ttcacttgct aacctagaat gatttgtgga ttctaatttg
      721 tcgattacaa gataggcaag atccttgctc atcccttgcg tgtatttgta tggtgtacca
      781 agtttgagag agcgtaacga aatttcagta tttgcgattt tttgtacaat tttttgggat
      841 ggaattcctt caacgttagt gttatgttgt ttcttcgcta gtaattctag tgcaatttgt
      901 tttctttttg ttaagagttt gaactgggta acgaatcgtt ctactgaatt tcctccaaat
      961 gtgcaaacgt agtacggtct tttaattttg gcaccattag ttgcacactt cattgcaacg
     1021 cgtattcgaa gccaaattcc aaagtgacgg agaatgtcac gcatgccgta tgcagcattt
     1081 ctgctcgctg ttgtaaattc acaatgtgtt ttccccacat ggccttcagc gtcaaacatt
     1141 gcgtgaacaa atgctttttg tgcttctaat gatgattcta acacagactt tggaattatt
     1201 ttatctgcag attttttacc ccattgataa ccaaaccttg taaagaggga atgaagtctt
     1261 gttgttgaaa ttacaaatcc tctgccgttt ctttttgcta ttggtagtac aattccatat
     1321 tttcttgcaa gttcatgcac cctagataaa acaatgggat ccgaattgcg gaagataccc
     1381 actgcatttc cttctttagt gtaaaattca tgcccttcag caagctgcca tgccataagc
     1441 tcagctaaat ctggctcaag gttatttttt ccagaatgta acgtttttgc aggaactgcc
     1501 accttgtcat cgacagtaag ttctcctgcc atctgccacc cttgtggagt caaaaatcta
     1561 tgacgttgtg tacactctat gacagaacca tcccaaaggg tgagtttaac aattggttct
     1621 gagatttttt gtctgaaaat tctggtgatc tttttggcag ttattttttg gccatcaaag
     1681 gtttggattg ttattggttt tgatagtttt gcccaagttt catcgccatt ttgttctata
     1741 actgaggatc cattccataa atcaccaata tgtacttgac cattaattgt ctcaataaga
     1801 gatgtgccaa cagtgcattt tccaaggccc atttcgtctg caagtaatgc gttgcctgaa
     1861 gacttgatca aaaaatcaag cccttctctt tgaaagttaa acagagttcc tttgaactgc
     1921 ttgccaggat ttgcaagacg gagtttttct attctttttg gcttttttgg cttgtgctta
     1981 aacgatggag gaatctttct ttgccagacg ttttttgata aaatctcaag cgggtaccta
     2041 tccattatcc attttatctg ctgcaggttt tttggatcgt ctggaacaat tgcttcaaat
     2101 tcatggtctc cataccatgc ctgtggaatt atgcgagaca ccattgccac tgcgcgctcg
     2161 ccagtaattt tccagctcca tgtgccagaa aacttgtcca ttacatactc gagggtacca
     2221 aaatttgtca tgacgtcgat tatcccccgt agggtagttt cttcggttta tgaatcttaa
     2281 gttacaagca ggatctgact aaactgacca tttcaaaaat ttatagaaaa catacccaat
     2341 tgggtcaatt ttttactatt gactagatta ctcgattatt tttctaaata attaccataa
     2401 attgctaaat tctacatgtt tttaccaaag ttttaatcgt ttagggacgc acataaaatt
     2461 gtgaagttta cgcagagttc agaaccagtt gtggaaaaac cactacttgt tgccgcctta
     2521 caagacatgg gaaacgttgg caatatagta attgacttca taaacaaagc aaaaaagtca
     2581 acgcccttta gaacggcaga atcaacccaa ctatcatatg ttttggacaa gggcggtcac
     2641 atcgaaatac caaaggaaga ctgggagtac cgctacacaa aaaacatgat agtctttggc
     2701 ggcggatatg gacagccaaa gacaaatgag gagctccacg cgctttgcca ggacgtaatc
     2761 agcgtggcaa aaaaatacac ggccaaattc atctacaccg ttggcggctt tcacacagaa
     2821 cacccaatag aaggcgagcc gaaaaccttt gtcaccacca cgtctgaaga cctctcaaag
     2881 cagctggaaa aaattggcgt aagcatgacc ccgtccaaat cagtcatcac tggctttaac
     2941 ggaatgattt tgggatatgc aaagctcaac gacatttccg gtataggcct atacggcgag
     3001 ctaaacgagc ctgcagttcc acagtaccgc acagcaaaaa gcgtcatcaa gacactagaa
     3061 aagctcacat accaaagctt tggcgaaacc caagagcttg acatgttggc aagcgatatt
     3121 gaaagaaagc taaggtctgg ctggcgggca gaattctagt tgtgcttgtg cgtttctgac
     3181 ttggataaga cgtgacagtc acagctacaa agctcagttt tatgatcatt ttggcagtca
     3241 atgcactcat agtgcttgtg tttttcgcag cttgcacata tcataccaat cattataccg
     3301 ttgccatatt ttagcactac ccctaaaggt ctatttcgct tttttccagt aatgatgctg
     3361 tgagcttgta ataagaaacc gggtcgattt cctttgcaaa gcccttgtca atgttgatta
     3421 gatatatcga gtccaatttt gcattgagga tgtcgtcata ttctattgga gggtttctgt
     3481 agaatctgtc acatcccgtc ttggtttttt taatctcagc ttctggccgt aatgatggcg
     3541 gatttaaaaa gattcggtga aatggaagta tcccaattac gggagtggca agtgaaaact
     3601 cgttacaaaa tttgctatac cttgctatct tgctcactat tctggacaca tagtaatcaa
     3661 tcgtatcaag ggcatgctcg gggggagtaa agccagtctc tatttcgatg attgtagtat
     3721 gctcatcttt ttttgcataa acatcgcata caagtgagtc agtaagctgt ttttcaacat
     3781 ctactgcata tcctctcgag accaagtttg ctgcacagat tagctccaaa acagaatggt
     3841 tgatctttac caagttctgc tcatacagct caattaaccg ctgtctgaca tagtttagtt
     3901 ttggtatgtc gtctttttgc agctgtgtgg aaagcttatc ggtagccaca tagacatcat
     3961 cgcgaaattt ttctaaatcc aaaattcaac actaatgcca accacttggt ttatgaatat
     4021 gagttattga ttttgttggt cttgggtgtg aaaactagct aaaaaagaac gattggttat
     4081 actggttctc taacactttt gactttggca attgcagtcg gatcaccatt aattggtcca
     4141 acggtaattg ttacggtgtc ttgtgctgtg cgtccagttc cgtcgctaac cataagctca
     4201 aagactagtg attttgtttc accgtttgct acttggggtg ccacaaatga tggcttggca
     4261 agatcagatg ctgaaagtga tacttcctcg cctgagagct gtctccagtt gtagaacagt
     4321 ttatgcttca ttgggtcttt gcccgagcca aataatgtaa cgactgattc ctcatcaaca
     4381 ttttggtcac tgccagcata tgctgttagt ttcttgctaa ttaggccttt aactacaaca
     4441 tcaacagtag atggttcgct gtcggcttga ccatcgtttg cgattagctg gaatgtaaat
     4501 gtcgtatcaa cttcaacgct tggtgctacg aaatccgtag tcaagtcagt ctctgctgaa
     4561 agtgtaacgc ttgggccaaa tatttggctc cacagatatg ttaatggatc gtcatcctca
     4621 tcgcttgcag aaccacttag tgtggttgta atgccaatgt caactgtttg accatcgcca
     4681 gcatcagcta ctggtgctgc gttaactgga agaactgtaa ctctggtcgt atcctttgcc
     4741 ggatgtccgc tttcgtcaga aactctgagc tcaaagacta gcacttttgt tttgccattc
     4801 tctacagtcg gcgctacaaa tgttggttct gcagcatctg ttgcggatag ttctactggc
     4861 tcacctgcta cttgtctcca ctcaaatgtt atttcgtcac tgtcaggatc ctcacctgtg
     4921 ccggcaagtg tgacctcggt gtgctcatca actacttggt cggctcctgc atctgcagac
     4981 gctctaacat tatcagactt tactgtaatt actacttgat cagaatcttc accgccatat
     5041 ggatcagtta ctgttaattc gaatgtaagt gtggtcgatg cgccaccaaa cacctctggt
     5101 gtcaggaatc tcgcatcagc tttgtttgga taaattacat ctacatcagt accagatact
     5161 tgtttccaag aatactgtaa tctgtcaccg tcggggtctg agccagaacc acttagatac
     5221 aatattctaa aggacattgt ttccttgtct gggcctgcat ccgcagttgg atcatcattt
     5281 attggtctta ctctaataag tacaatatcg gttgcagtac cgccaccctc tgctgcatca
     5341 caagtaaatc taaatgccaa tactacaatt gcaccgtttg gaatttctgg cgcaacaaat
     5401 gttgggtcag cgtctgcagt tgatgataga tctgcaaatg gtccaagtgt ttgagtccac
     5461 gtataagaaa gatctctatt caatttgtca gtgcatattc catctagttg aacggtttgt
     5521 ccttcgttaa ctgttttgtc agtaccggca tttgcagaaa tcagagtcgg ttttccagct
     5581 gtaactttga taattactgt gtctcttgct agtccaccaa agccatcagt tactgttagt
     5641 tgatatctca aagttcctga acttctcgtc attgcagaag tatcaagtgt cggactagat
     5701 gagtttggat catccaagtc cactattggt ccgtcaattt gcgaccaacg gtaagtcagt
     5761 gggtctccat ctggatctgt accactgcct cggagtgtta cctcatcgcc tttttctact
     5821 gtctggtcag agccagcatt agcagttggg ggttggtttc ttggcataac ggtaattttt
     5881 atgatatcag tgtcaacgcc gccgtatggg tctttaactg ttaattcaaa ggtaaggatt
     5941 atgactttgc cattttcaac ttctggggcc tggaatgttg gttctgcaac acctgttgat
     6001 ggcataagag taacttgctg gccaccagtc tgtctccaca taatagtcag tgaatcatca
     6061 tctggatcta ctccttcgcc ttgaagggtt acaaagtctc cagatttgac aatgttctga
     6121 gctggggtgt aactagttgc accaagggcg gaaggtgaca aactgctcaa aactatcaaa
     6181 gtcagcaata gtgtgtagaa ttttaattgc aacattttta tcgtaaaaat tacaccgatt
     6241 aaaagctttc gttcaatttg gggacatttt tggatcaatt ttttgtcttt gcaagttttg
     6301 ttttgccatt tgttaagaag cggcccgata cggtcagata tcgatcccat gtcggtttgg
     6361 acgcagatta gatgttttgg tagatcagat aacgcaaaaa catgatcgtt tttgatgact
     6421 caaaaataac tgatttgaaa ctgttatgat tttgatctgt ggggttaaca aaaaaagccc
     6481 gaaaaatcga caatgcgccg ccacattgta atgggtatgt tattagcagt cattttgctc
     6541 ggaaacgcca ccatcgcttc tgctgcaaca ccgtgggatt tgatcgtcag ggcaaaccta
     6601 gagcaagcag aagttagcct aaatggaaag cctgttgttt ttggtacagt tttagatcaa
     6661 gctgcgaggc cagtctccgg agttgaagtc aagataagct tttctgatag gtcagctact
     6721 accacaaccg attctgaggg caattttaga tacgagtttg gtgagcagta tatgccaggc
     6781 acattctctg tcaacgtttt tgccaagttg gcagacaaga aaggctttgc aaaactgacg
     6841 ctaaagatcg gcaatgagag cacgacgttt agcgaaattt attatagctc agacaagttt
     6901 gctggcagta caacggcatt accaaacgat ccatatgccg caataaagct aaagcacctc
     6961 aaagaattca ttgaggagca aaacaggata aaacaaaaac agatagacgt tgaagccaaa
     7021 aaacttgcct tgcatggaaa acgaagcttg gcccaacaaa tgcttgaaaa ggcgttaagg
     7081 gaacgcccag ttggagcagg agttttttct gatgcacagt atgaaagata cctagcaaca
     7141 gtagatccta ggattaaaga ccagatatca tatcagttaa actatacaaa acaactgcgt
     7201 gttgaggcac aacatgcaat gaaggccgta ttagacaatg gtggctcatt agaggatgca
     7261 agaaaggcgt actttgagaa attatcagtc acaaaagagg agcttcttga cgtcaatgac
     7321 ctgaacaaca ctaagaatta ctccaaaatc aaaaccaatg acgataaaaa gatcaacagt
     7381 aagaaagtaa aaggattaac agtaaataaa aatctaaaat gatcgagttt tgcgtctttt
     7441 ctgcagttcc aaattggaac taataaggca gcattatttc cgtgaaagat ataatcgaag
     7501 ctaaagaaac ccggccatga tgtctacaag catcagtcta ggcaagggaa ttggaaagca
     7561 gctttcctca atactaatag acgaggaagg cgtttcgctt gagaacattt gggaaaccat
     7621 cgctgatttt ggcataaaca gggatgacct aaaaaagcta caaccgacgt acaaggaatt
     7681 atttgagatc tattctgcca tcaaggcata cagaataagc gtcaaatacc tcaaagagct
     7741 caaaaacaag ttttagcctt tgcctaatct gggctggcat caggaaaaga aaaaatggac
     7801 ataaatgact taaaagaaaa gctggttttg ctgagcatca ctgcggtagt ttttttgcca
     7861 ctgcggctat tggtcagcca atacatctcc gatcactggc ttggcaacct tggcatcgcc
     7921 acactgattt ccgtgagttt gatagtttta gtaaggaaaa acaagcttgg cagatttggc
     7981 catgtgttca aaaaccaaat cacaaaggcc ttgtggggac ggtcagccaa actcatcatt
     8041 gccgcacttg tgttttttat gatttatttt ggtacgactg tgcttctaat agacagggga
     8101 aacacaattt atctagatga caaggaaatt ctatcgcaga gtatcgctag tagaaatctt
     8161 gacaggactg cacttttgag cataagtggt cctcaaatac acagtattat cggtcttgcc
     8221 cagatacaat acttggaata tttgttttct gtatcatatg caattttaaa cgataccaca
     8281 aatggctggc ttgttaattt gcatttgata ttattcatgg aacaagttga agtgttgggc
     8341 cttttctggt tttacaagag aatgtttaag ccaataccta cagatgccac ctaggcgctg
     8401 tttgtctgtt ttggacagac aaattaagaa tatcattttg gttttgagta gacttggaga
     8461 caatttgtat aaagcacttg cagtattttc gtttgttttg attctactat attccaatta
     8521 tgtctatgct acaggtgatg atttggaaat caaagttcta aaggggtctt cagacgaaga
     8581 cttgcaaatc aagtcgtttt atcctgagat tttgccgatt gcgccaaacg atgccataac
     8641 ttgggtaaat gatgactctg caacacacag cattacgagt ggcatctcag ggcatccaga
     8701 ttattcagga aaatttttca agacaggtaa catcgcccca tccaagtcct ctacagtaaa
     8761 gatagcagac acatcaagtt ttgcctatta ttatttct
//
LOCUS       DASWKH010000066         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_543953, whole genome shotgun sequence.
ACCESSION   DASWKH010000066 DASWKH010000000
VERSION     DASWKH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543953"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGA92_04970"
     CDS             <1..372
                     /locus_tag="VGA92_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEX9845799.1"
                     /translation="GLTGPQGSQGEKGPRGPQGPQGEQGPQGAPGEKGPQGPQGIQGP
                     QGERGGPGPRGPPGEKGPHGPQGSSGDKGPRGPPGPPGPPGTAGLSDEQKALFRELLE
                     VLTAKNIITTEEQIKLMSYLY"
     gene            425..676
                     /locus_tag="VGA92_04975"
     CDS             425..676
                     /locus_tag="VGA92_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845800.1"
                     /translation="MLDYERTGNDFVAFKLEDGALVKVKVDLDRVGIATNHKNPDGTP
                     HYAINTSVKITIIPNNRTFSVDKNLKEKHTPPPPGQMFS"
     gene            complement(966..1103)
                     /locus_tag="VGA92_04980"
     CDS             complement(966..1103)
                     /locus_tag="VGA92_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845801.1"
                     /translation="MMKTKQEIIFIPQTATYKRRRQYKCTICGHTSWLQGKRPSAESV
                     Y"
     gene            complement(1168..2271)
                     /locus_tag="VGA92_04985"
     CDS             complement(1168..2271)
                     /locus_tag="VGA92_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9845802.1"
                     /translation="MGIPEKIKAIQDEIHKTQLNKATEHHVGLLKAKIAKLKREQEKN
                     QAKKSGVSSEGFDIRRTGDATVVFIGLPSVGKSTLLNKLTGSRSAVAAYRFTTLTVVP
                     GMLEYRGAKIQILDLPGIIEGASKGKGLGKRILAVARSADLVILILDVFQPYHENVLR
                     TELGNIGIRLDQKPPNIIIEKASTGGIAIAQQVKLTLLPEKLLKEILHLYGITSARVV
                     IREDITSEQLVDYLTGDKKYAVSLTVLNKIDLVDPKFVKQVQTKVKSDVIPVSADSGI
                     NIDLVKEKIYEKLDFIRLYMKPKGGETDYKEPLIIRRNSTIEDVCNKIHRSLKKEFRY
                     GLVWGKSVKFGGQRVGLTHVLEDEDVLTIIKSR"
     gene            2349..>2879
                     /locus_tag="VGA92_04990"
     CDS             2349..>2879
                     /locus_tag="VGA92_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX9845803.1"
                     /translation="MKRKTFAVDIDGTITENGGGRIHLGALNSLRHLRRLGHNVIFVS
                     GRSSVEGYLLAVYGGLNTIAVGENGGCITKGANDHILLGDKEKCVEAFSLIRSKLSNV
                     IEKPVFPRMTEVVLQRTFDINDAKKIVQNNNLDVLLSDSQYAIHINSSGINKATGFGE
                     IMKTLGITSDDVVCIGD"
BASE COUNT          819 a          575 c          591 g          894 t
ORIGIN      
        1 ggtcttacgg gtccgcaagg ctctcagggt gaaaaaggtc cacgaggtcc acagggtcca
       61 cagggcgaac aaggcccaca aggcgcacca ggtgaaaaag gtccacaagg cccacaagga
      121 attcaaggcc cgcagggcga acgagggggt ccgggtccaa gaggcccacc gggagaaaag
      181 ggtccacatg gtccacaagg ctcatcgggc gacaaaggtc cacgaggccc accaggtcca
      241 ccaggcccac cgggaacagc tggtttatca gatgagcaaa aagcactgtt cagagaattg
      301 ctagaagtgc ttactgcaaa aaatatcata actacagaag aacaaataaa actaatgagc
      361 tatctgtatt agctacttgg gctgatgcta gttgagtgaa ccaaaaaata aaatagaatt
      421 taaaatgttg gactatgaac gtactggaaa tgattttgtt gcattcaagc tagaagacgg
      481 tgcactagtt aaagtcaaag ttgatcttga cagagtcggc attgcaacaa accacaaaaa
      541 ccctgatggg actcctcatt atgcaataaa cacatcagtt aagatcacga taatcccaaa
      601 caacagaaca ttttcagtgg ataaaaacct taaagaaaaa catacgccac cgccaccggg
      661 acaaatgttt agctaacaaa atttagaaaa ataataaatt tgcgtcaacc ataattgacg
      721 acttttgacg aatttatctt cgtcgcttct tagtagctac tttctttcct tttcgcttag
      781 ctgcctttcg ttttggtgct gcgcgttttg gagctttgcg ttttgctttt gcttttcgct
      841 tagttgccat tagcaaaaat atcttttact agtttttcta tggtaatact aaacaaaatt
      901 acaccaagtg attacaaatt gtaaacgatt tttttgcaac gaaaattttt ctgatcgcaa
      961 aaattttagt aaacactttc agctgatggt ctttttcctt gcaaccagct agtgtggcca
     1021 caaatcgtgc acttgtactg ccttctacgt ttgtatgttg ctgtttgggg aatgaaaata
     1081 atttcttgtt ttgtcttcat catacaaaat ttacaccaac ggttttcagt ttcatgatcc
     1141 atgtttgttt agaaaactga ttgtttttta acgtgacttg attattgtga ggacatcttc
     1201 gtcttctaat acgtgagtga ggccaactct ttgtccgcca aacttgacac tttttcccca
     1261 aacaaggcca tatcgaaatt cttttttcaa acttctgtgt atcttattgc aaacatcctc
     1321 tatggtagaa tttcttctaa taattagcgg ctctttgtag tcagtttctc caccctttgg
     1381 tttcatatac aatcgaataa agtcgagttt ttcatagatt ttctctttta ccaagtcgat
     1441 atttattcca gaatctgcag agactgggat gacatcggat tttacttttg tttgaacctg
     1501 ttttacaaac ttgggatcga ctaggtcgat tttgtttaag actgttagag atactgcata
     1561 tttcttgtct ccagttaggt agtccactaa ttgctcggat gttatgtctt ctctaattac
     1621 cactcgcgca ctcgttatgc catacaggtg gagaatttcc ttgagtagct tttctggcaa
     1681 taaggttagc tttacttgct gcgctatggc aatgccacct gtagatgctt tttcaattat
     1741 tatatttggt ggtttttgat caagcctgat tccaatattt ccaagctctg ttcttagcac
     1801 attttcatga tatggctgaa atacatcgag aatcaaaatt accaaatcgg cacttcgtgc
     1861 aacggcaaga attcttttac ctaggccttt tccttttgac gcgccctcga taattccagg
     1921 caagtccaaa atctgaattt ttgctccgcg gtattctagc attccaggaa ctactgttag
     1981 ggtagtgaac cggtatgccg ccactgcaga cctagaacct gttagcttgt taaggagagt
     2041 tgacttgcca acacttggca agccgatgaa aactacagtt gcatcgccag tacgcctaat
     2101 atcaaagccc tcagagctaa cgccagattt ttttgcttgg tttttttcct gttcgcgttt
     2161 cagctttgca atctttgcct tgagcaaccc tacgtggtgt tctgttgcct tgtttagctg
     2221 agttttatgt atttcatctt gaatggcttt tattttttca ggaattccca tgattcacta
     2281 aacaagctta agcttctaaa taaaatctta tttgcttgtt tattatcagg gtatacaaaa
     2341 gtgattttgt gaaaagaaag acgtttgcag ttgacattga tggtaccata acagaaaatg
     2401 gtggagggag gatccatctt ggtgcgctaa actcactacg tcatctgcgc aggttgggtc
     2461 ataacgtgat ttttgtttct ggacggtctt ctgtggaggg ataccttttg gcagtgtatg
     2521 gcggactgaa cacgattgca gtaggtgaaa acggtggatg catcacaaag ggcgcaaatg
     2581 atcatatctt acttggggac aaggaaaaat gtgtggaggc cttttcacta atacgctcaa
     2641 aactaagtaa tgtaattgaa aaaccggtgt ttccaagaat gactgaggtg gtacttcaaa
     2701 gaacattcga cattaatgat gccaaaaaaa tagtacagaa taacaacttg gatgtacttt
     2761 tgtctgatag tcaatacgct atccacataa attcctctgg aataaacaaa gctacgggat
     2821 ttggggaaat tatgaagaca cttggtataa catctgatga tgtggtttgt attggggat
//
LOCUS       DASWKH010000067         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2757205, whole genome shotgun sequence.
ACCESSION   DASWKH010000067 DASWKH010000000
VERSION     DASWKH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757205"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VGA92_04995"
     CDS             <1..394
                     /locus_tag="VGA92_04995"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9845804.1"
                     /translation="LLTTDHGYVIPREIAEIGLEKEFKDCMDVSKRVFDSMRRKIPQQ
                     AQYVVNFAYNYPFFMHLNLREACHLIELRTIPQGHIDYRQVAQKMFLSIKKAHPSISS
                     IIKFVDLNTYELERFESEKRIEEKRKAS"
     gene            436..846
                     /gene="msrB"
                     /locus_tag="VGA92_05000"
     CDS             436..846
                     /gene="msrB"
                     /locus_tag="VGA92_05000"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9845805.1"
                     /translation="MSREKVVKSEKEWKDALTDDEFEVCRNKATEPPFTGKYTYCKDD
                     GIYRCTCCSNELFSSDAKFDSGTGWPSFFQPIHENSLKYETDTAYGMLRTEVMCAKCD
                     AHLGHVFEDGPAPTNNRFCINSVSLKLDKENENH"
     gene            926..>1797
                     /locus_tag="VGA92_05005"
     CDS             926..>1797
                     /locus_tag="VGA92_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9845806.1"
                     /translation="MRIIICGFGTVAQSLAKLLVSRSDDLYAKYGIKPRIVGAFDSKG
                     GAVASSGLDLNRLVEVKKKYGTIKNYDKTKKSLTGLDIINNVDADVLIETTASNYKDA
                     EPGMSHIVTAMKRGMHVISVNKGPLALAFPSLMELAAFNQVLLRFSGTVGGGTPILDY
                     AKNSLRGEQITSFAGILNGTTNYILTNMTRGMSFAAALKDARAKGYVEADESLDLDGY
                     DAAAKLVILANWIMGMNVTLPDIKRTGIRDVSSKDIKAAAKKKSAIKLIASCNKELIV
                     SPREVSIDDPLCVNG"
BASE COUNT          611 a          331 c          397 g          458 t
ORIGIN      
        1 gctactcaca acagatcatg gatatgtcat ccccagagag attgcagaga ttggtcttga
       61 aaaagaattc aaagactgca tggatgtatc aaaacgagtt tttgattcca tgcgacgaaa
      121 gattccacag caggcccaat acgtggtaaa ttttgcgtac aactatcctt tctttatgca
      181 cctaaatttg cgcgaggcct gtcatctaat tgaattaaga acaataccac agggacatat
      241 cgattatcgc caagttgcac aaaagatgtt tttgtcaata aaaaaggcac acccgagcat
      301 ttcatcgata atcaaatttg tggatctgaa cacttatgag ttagaaagat tcgagtctga
      361 aaaacgaatc gaggaaaaaa gaaaggccag ttaagttgga acaatcctat aatatggcat
      421 cacccaagtg attttatgag tagggagaag gttgtaaaat cagaaaaaga gtggaaagat
      481 gctctcactg atgatgagtt tgaggtttgt agaaacaagg caactgagcc tccatttact
      541 ggaaaataca catactgtaa agacgatggg atttaccgtt gcacatgttg cagtaatgag
      601 ttgttcagct ccgacgcaaa atttgactct ggaactggct ggccaagctt tttccagcca
      661 attcatgaaa atagcctcaa gtatgaaaca gatacggcct atgggatgct aagaaccgaa
      721 gtaatgtgtg caaaatgtga cgcacatctt ggacatgtct tcgaggacgg acctgccccc
      781 acaaataatc gtttttgcat aaactcggtt tcattgaagc ttgacaaaga aaatgaaaat
      841 cattaataaa ttagcactta atcaagtcat ttgggttgag gcctaattca gttaatattt
      901 caataaaact gtggagtaga aaaacttgcg aataatcatt tgtgggttcg gtacagttgc
      961 ccaaagcttg gcaaagttgc tagtctcaag atcagacgac ctttatgcaa aatatggaat
     1021 aaaaccgaga attgttggtg cctttgacag caagggtgga gcagtagcat catctggtct
     1081 tgatctgaat agactagttg aagtaaaaaa gaaatacggc acaattaaaa attatgataa
     1141 aacaaaaaaa tcgctaacag gacttgatat tattaacaat gttgacgcag atgtgctaat
     1201 agaaacaact gcaagcaatt acaaagatgc agagcctggc atgtcacaca ttgtgactgc
     1261 aatgaagaga ggcatgcatg tgatttcagt caacaaggga ccactagcac ttgcatttcc
     1321 atcactgatg gagcttgcag cgttcaacca agttttgcta cgatttagcg gaactgttgg
     1381 tggtggaact ccaattttgg attatgcaaa aaacagtcta cgtggtgaac aaattacctc
     1441 ctttgcaggc atactaaatg gcaccactaa ctacattttg acgaacatga cgcgtggaat
     1501 gagttttgca gctgcgctca aagacgccag agcaaaaggc tacgttgaag cagacgaatc
     1561 ccttgacctc gatgggtacg atgctgctgc aaaacttgtg attttggcaa actggattat
     1621 gggaatgaat gtgacactcc cagacattaa aaggaccgga atacgagatg tttctagtaa
     1681 ggacataaag gcagctgcca agaaaaaatc agccataaag ctgattgcat catgtaacaa
     1741 agagcttatt gtgagcccaa gggaagtatc aatagacgac ccgttatgtg tgaatgg
//
LOCUS       DASWKH010000068         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_560554, whole genome shotgun sequence.
ACCESSION   DASWKH010000068 DASWKH010000000
VERSION     DASWKH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560554"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VGA92_05010"
     CDS             complement(<1..1799)
                     /locus_tag="VGA92_05010"
                     /EC_number="1.2.7.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018491 - 2-oxobutyrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0047553 - 2-oxoglutarate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX9845807.1"
                     /translation="MIGGPQGSGVESGANIFSRACAALGYQIFGKREFYSNIKGEHSY
                     FGVRVSDKTIRSNVNDVTLMVAFDAETIFRHYSEVIKDGAIIYDSDLSVIRVDEVHTL
                     DDQYKSRLLAHLASKNKKPTVQDVLEMAKENGVKLYPISFKDVLSKMADETNNPRLKG
                     MIRMYNVLGVSFSLGVLKMPPQTLLESIDVIFAKKQAIADLNKKAANYAYNYASARFS
                     DFTFSLNETNKKPGTLLVQGYQGTALGKMACGCRFQPYYPITPASDESVFLESNEIFE
                     VQNNRPGHTLVVQTEDEISAIGMTIGGALTGARSATCTSGPGFSLMAEAMGWAGINEV
                     PIVVTLYQRSGPSTGLPTRHGQDDLLFAIYAGHGEFPKIVYASGDIEESFYDTARCFN
                     YAEVYQLPVIHMMDKFLASSVITCDKFDSDKITINRGKLLDKIDGEYKRFAFTQDNIS
                     PRSKLGLENGIFWNTGDESDEYGHISEDPESRIKMMDKRNSKLDYALKTIPDEDQVVS
                     FGAAETCIVSWGSPKGPILDALEMLNQENIKIGFVQIKLLNPFPADYVKFLLKDAKTI
                     VDIEANQTAQLGQLLKQHLERGADYYILKYTGRP"
BASE COUNT          476 a          428 c          406 g          645 t
ORIGIN      
        1 ggccttccag tatatttgag aatgtaatag tcagctccac gctctaggtg ttgttttaag
       61 agctgaccta gctgggcagt ctgatttgcc tcaatgtcaa ctattgtttt tgcgtctttt
      121 aggagaaatt tgacatagtc agctggaaat ggatttaata gctttatctg aacaaagcca
      181 attttgatat tttcttgatt tagcatctct agcgcatcaa gtattggccc ctttggtgag
      241 ccccagctaa ctatgcatgt ttctgcggct ccaaacgaaa ctacttgatc ttcgtcagga
      301 attgtcttta gtgcatagtc aagctttgag ttgcgtttgt ccatcatttt gattcgagat
      361 tctggatctt ctgaaatgtg cccgtattcg tcgctttcat caccagtgtt ccagaatatt
      421 ccgttttcaa gtcccagttt tgatcgtggt gagatgttgt cctgtgtaaa cgcaaatctt
      481 ttgtactcgc cgtcaatttt atcaagtagt tttccacgat taattgtaat tttgtccgag
      541 tcaaatttat cacaggttat tactgagctg gcaagaaact tgtccatcat gtgaatgact
      601 gggagctgat acacctctgc atagttaaaa catcttgcag tatcgtagaa gctttcctcg
      661 atgtctcctg acgcataaac aatttttggg aattcgccat ggcctgcata gattgcaaat
      721 aaaagatcat cttgtccgtg tcttgttggc agtccggtag acggtccgct cctctgatac
      781 agtgtaacta caattggaac ctcgtttatt cccgcccacc ccatggcttc tgccataaga
      841 gaaaatcccg ggccagaagt acaagtagca gatcttgcgc cagtaagtgc gccaccaatg
      901 gtcattccga ttgcagagat ttcatcttct gtttgtacta ccagagtatg gcctggcctg
      961 ttgttttgga cttcaaagat ttcgtttgat tccaaaaata cgctttcatc ggatgctggc
     1021 gttattggat aatacggctg gaatctgcaa ccgcaggcca ttttgcccaa ggcggttccc
     1081 tgatagcctt gtactagaag tgtaccgggt tttttgttag tttcatttag cgaaaatgta
     1141 aagtcgctaa atcttgccga ggcgtaattg tatgcgtaat ttgccgcctt tttgttaagg
     1201 tctgcaattg cctgtttttt tgcaaatatc acatcaattg actctagaag tgtttgcggg
     1261 ggcattttta gcacgccaag agaaaatgac acgccaagaa cgttgtacat tcttatcatt
     1321 cccttcagac gcgggttgtt agtttcatct gccatttttg agagaacatc cttaaacgat
     1381 atcgggtaca gttttactcc attttctttt gccatctcca gaacatcttg gacggttggt
     1441 tttttgttct ttgaagcaag gtgagctagc agtcttgatt tgtactggtc atcaagggtg
     1501 tgtacctcgt ctacccttat gacagaaaga tcggaatcat agattattgc tccgtccttt
     1561 attacttcag aataatgtcg gaagattgtc tcggcgtcaa atgcaaccat cagtgtcaca
     1621 tcatttacat tcgatcgaat tgttttatca gaaaccctaa cgccaaaata gctgtgctcg
     1681 ccttttatgt tggaatagaa ttctcttttt ccaaatattt gatatcctag tgcggcgcat
     1741 gcacgcgaaa agatgtttgc tcctgattct accccgctgc cttgagggcc tccaatcatc
     1801 caggttatat caacagtcac aggtctccta tgatttgttg attattaaga atatgtttaa
     1861 ttttcttacc caccggtagc agcctcaaaa agggcgcact cgcacttgtc tcgctcgccg
     1921 cagtatggac atacgcaaac gcacttttct attgc
//
LOCUS       DASWKH010000069         7134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2044812, whole genome shotgun sequence.
ACCESSION   DASWKH010000069 DASWKH010000000
VERSION     DASWKH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7134
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044812"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGA92_05015"
     CDS             <1..744
                     /locus_tag="VGA92_05015"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9845808.1"
                     /translation="IIKHFDHDGQKLIALGGVNLTVEDGDFVCLVGPSGCGKSTFLRI
                     AAGLQKPDEGQILLDNRPINNTGPDRILVFQEGALFPWLKVRDNVEFGLKIAGIPESE
                     RHQISDRYLEMMQLTKFANSYTYQLSTGMKQRVSIARALVMDPAVLLMDEPFASLDAQ
                     TRDLLLVEMQMIWQKTKKTIVFVTHNIAEAAILGNKVAVFTHRPSTIKKIIPVDYPRP
                     RLSEDEGLAKFQQEILTELKPEVKARGME"
     gene            741..1499
                     /locus_tag="VGA92_05020"
     CDS             741..1499
                     /locus_tag="VGA92_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9845809.1"
                     /translation="MKLNVTAKRIAFYAGIILVWQALDVANIWSDNVFPSPYEVAEDL
                     WYGAIDGTLWLGIGTSLWRLVVGLAIAIILGMILGIFMARVPTVNQTIGSIVLGLQSI
                     PSVAWVPLGIVWFGLTDAGIIFVAVAGSLFAVTINTYSGVKNINPDYIAAAQNMGARG
                     GQLVTKVLIPAAFPYMITGFKQGWAFAWRGVIGAELLFSFLGLGFLLNVGRQLNDVSQ
                     VIAMMFVIMLIGILIDGLVFKRLEDKVMSRWGLR"
     gene            complement(1517..1720)
                     /locus_tag="VGA92_05025"
     CDS             complement(1517..1720)
                     /locus_tag="VGA92_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845810.1"
                     /translation="MTKVKKSITISGELDSIIAMLASKLDQTTSELIEFRLREDPQIK
                     AMLQELRAASEPPEYEKTKKALN"
     gene            complement(1860..3044)
                     /locus_tag="VGA92_05030"
     CDS             complement(1860..3044)
                     /locus_tag="VGA92_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9845811.1"
                     /translation="MEQPFVVGKPVTGKYFIDRKSELKQLLALLSGTPKGDINNVILL
                     GLRRTGKSSILLNAKKTLDKNKKIVPVIFDAYGISTKERFGRTFISKVLQTYVEKSGE
                     RSYKERIKKLLEEKYEKLAERVSEFDVEFAEYIKFQIKLREPKVNEDELLEAALQYPE
                     KLGKDKGVYFVIMIDEFQELLQWDKDFLKMFRRLVQAQKHVAYVLAGSAPTIMKQMVY
                     DVSSPFYKQLTEIRVGKLSQESVSSFVQKRLKSVKISIDKKALEVVFYLSHGFPDYVQ
                     RLGLQIFLRALESGKKQVEESDVQVAYSEMILQLDPDFNNNFSTFSPLEKEILIALAT
                     GSKSPAEISSEIRKPMSTLPKTLTRLMNQDVVEKYQEAKYRITDPILSDWLNRRYGQL
                     TM"
     gene            3364..4431
                     /locus_tag="VGA92_05035"
     CDS             3364..4431
                     /locus_tag="VGA92_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845812.1"
                     /translation="MDETTYRILDVLSRNLGKPISIHELTSKIQEAYGAAYYANIHEK
                     IGQLSRERMIELLRAGRSSLVFLNFENYLLIDLLAEMELKRKRDFLSGKKEMQMLMME
                     MDTLVHNMPFIKSVTLMSPQRNAKLNKAELLFCINKSDRKSTEETKISINRITANLQK
                     AYNIRIDHLILEDKDFVDLLRSNENNPAKEMLHNKISALHPQNFWMSISGAIRGGIKI
                     VAEESQTNPAKITEEDLVFNLARFGYVELGSKIKQGRLICIGYIIAAIMFKNDARRMD
                     AIPVILAKNSANYNLVLFLARKYGFEGKILGILAALRNLIGNKMANIDEPVRLLEAMK
                     IKEIRADKKGIKEKLRLYNVA"
     gene            4421..4606
                     /locus_tag="VGA92_05040"
     CDS             4421..4606
                     /locus_tag="VGA92_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845813.1"
                     /translation="MSLDNTKLLDFLGEIDKEPEHRIVIVAVGGTAMTLECQTIHHRC
                     IKSIVQASVYWLILNTY"
     gene            4656..5264
                     /locus_tag="VGA92_05045"
     CDS             4656..5264
                     /locus_tag="VGA92_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845814.1"
                     /translation="MAKSPPKRGQVKERILRVILSHTNEKLTKYRIMKLAKANISWVI
                     EFLRVLQSKGLVEGTSIKDYKGLIVYWKKMRIKPESREYLIQQPLEVLRNANLQYALT
                     TYYAENLVQKYLFTSRIDVYISKNDFEIWHKLLSKSGLVGKGNVRVLVDDEHVFYKSS
                     ERAGLKIVSMPQLIVDLLVEGGPCEEAANMLIQKEIDNVSKP"
     gene            5248..6033
                     /locus_tag="VGA92_05050"
     CDS             5248..6033
                     /locus_tag="VGA92_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845815.1"
                     /translation="MFQNHELDISKSNLTTVFSKMREPVCLLGGWAVHLTVNQNFKDV
                     TGRDYIGSRDIDLGFHIDPDWSTTELQNSAFAQSVKTLVDNKFVGCGSRFVKHYDIST
                     LKELTEDQTRKKPMYEMFQLFVDPIVDNIHRDSQKLGFTPLDESLLSHVFNGKKFTSL
                     SGFGGKFMLPTSEILLATKISSVPKRDMVHKRIKDIADIYALSWHSGLKFEDLKQKLQ
                     QIVDKVVISDVVSTFTKSDYDAVFNAIGVTQEQVSRVIGELAK"
     gene            complement(6397..6573)
                     /locus_tag="VGA92_05055"
     CDS             complement(6397..6573)
                     /locus_tag="VGA92_05055"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX9845816.1"
                     /translation="MAKKFACGDVMSGCAWSATADDESELFAKITDHAKHDHGITVIP
                     DEAIQKIKSKIQDI"
     gene            6672..>7134
                     /locus_tag="VGA92_05060"
     CDS             6672..>7134
                     /locus_tag="VGA92_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845817.1"
                     /translation="MNTKQIGTLTAITAAALVFANINVAFADSYKNYDKRAIHAMEVK
                     QAQAASKMVKILEAYKLTLTEHSFDNKATEKRVAGNAKSIDNQITGIVDLLKNRSQPL
                     VKALTAPSAEILKIAKFGSISQGGSEVMRVTYKVTAGNYDLRDVKAVVGSET"
BASE COUNT         2189 a         1401 c         1551 g         1993 t
ORIGIN      
        1 attatcaagc attttgatca cgacggccag aagctaatag cgcttggcgg agtaaacctc
       61 acagtagagg atggcgactt tgtgtgtctg gttggcccat cggggtgtgg caagtcgaca
      121 tttctgagaa tcgcggcagg actgcaaaag ccagacgaag gccagatact gcttgataat
      181 aggccgatca acaatactgg tcctgacagg attctagtat tccaggaagg tgcgctgttt
      241 ccatggctga aggtccgcga caacgtagag tttggactaa agattgctgg aattccggaa
      301 tcagagcgcc accaaatctc tgataggtat cttgagatga tgcagctaac caaatttgca
      361 aactcgtaca catatcagct atccactgga atgaagcagc gtgtttccat tgctcgggcg
      421 ctagtaatgg atccggcagt attgctcatg gatgagccgt ttgcatccct ggatgcgcag
      481 actcgtgatc ttttgctagt ggaaatgcag atgatctggc agaagacaaa aaagaccatc
      541 gtatttgtca cacataatat tgcagaggca gcaatccttg gaaacaaggt agcagtattt
      601 acccacaggc catctactat taaaaaaata attccagtag actatccaag accaaggctt
      661 tcagaagacg aaggccttgc aaaattccag caggaaatcc tgacagagct aaaaccggaa
      721 gtaaaggcaa ggggtatgga atgaagctaa acgtcactgc aaaaagaatc gcgttttacg
      781 caggaatcat cctggtgtgg caggcactgg atgtggcaaa catttggtcc gataatgtgt
      841 ttccatctcc atacgaggtt gcagaagact tgtggtatgg tgcaattgat ggaacactat
      901 ggcttggtat tggaacaagc ctttggaggc tggttgtggg tttagcaatt gcgataattc
      961 ttggaatgat tcttggaata ttcatggcac gggttccgac agtaaaccag acaatcggct
     1021 caatagtttt gggactgcag tcgattccat ctgttgcgtg ggtaccacta ggcatagtct
     1081 ggtttgggct aacagatgcc ggtatcatat ttgtcgcagt tgcaggatca ttatttgcag
     1141 ttaccatcaa cacgtactct ggtgtcaaaa acatcaatcc tgattatatt gcggcagcac
     1201 agaacatggg cgcaaggggt ggccagctag tcacaaaggt tttgattcct gctgcattcc
     1261 catacatgat cacagggttc aagcagggct gggcatttgc gtggcgtgga gtgattggcg
     1321 cagaattgtt gttttcgttt ttgggtcttg gctttttgct aaatgtggga cgccagctaa
     1381 atgatgtatc gcaggtaata gctatgatgt ttgtaataat gctgattgga atcttaattg
     1441 acggcctagt cttcaaaaga ctagaagaca aggtcatgtc aagatggggc cttcgttaag
     1501 tgatcatttc cagtcgttaa ttcaaagcct tttttgtttt ttcatattct ggcggctctg
     1561 atgcagccct taattcctga agcattgctt tgatttgcgg gtcttccctt aatctaaact
     1621 caatcagctc tgatgttgtc tggtctaatt tagaggccag catagcaatg atgctgtcca
     1681 gctcgccgct aatcgtaatt gattttttga cctttgtcat actactatag tggcatctaa
     1741 gtaggataaa atgttatccg catgcctgtg ataggtacgt ttgaggattc ttgatttttt
     1801 gtaaatacct atcatcttag caatatgcgt ttttgaattt agatgcagat ttgcatatat
     1861 tacatggtca gctggccata tcgcctattg agccaatctg agaggatggg gtcagttatg
     1921 cggtatttgg cttcttggta tttttccaca acatcctggt tcattaggcg cgttagtgtt
     1981 tttggtagag tcgacatcgg ctttcgaatc tctgaggaaa tctcagcagg gcttttactt
     2041 cctgtggcaa gcgcaattag gatctctttt tcaagtggag aaaaggtcga aaaattattg
     2101 ttaaaatctg gatctagctg caagatcatc tcagagtatg ctacttgcac atcggactcc
     2161 tctacctgtt ttttgccaga ttcaagggct cgcaaaaaaa tctgcagacc cagcctttgc
     2221 acataatctg gaaaaccatg gcttaaataa aataccacct cgagtgcttt tttgtcaata
     2281 gatattttga ctgattttaa tctcttttgg acaaaggatg agaccgattc ctggcttagc
     2341 ttgcctacac gaatctccgt tagctgcttg tagaaaggag aactaacatc gtacaccatt
     2401 tgtttcatta tggttggcgc agaaccagct aagacatacg caacatgttt ttgggcctgc
     2461 actagccttc taaacatctt taggaaatct ttatcccatt gcagcaattc ttgaaactcg
     2521 tcaatcatta tgacaaagta tactcctttg tctttgccta atttttctgg gtactgtagt
     2581 gccgcctcaa gtagctcgtc ttcgtttact ttgggctccc ttagcttgat ttggaatttg
     2641 atgtattctg caaactcgac atcaaattcg gatacccttt ctgcgagttt ttcatatttt
     2701 tcttccagta attttttaat gcgttctttg tatgagcgct caccgctttt ttcaacatat
     2761 gtctggagga ccttgctgat gaacgtcctg ccgaatcttt cttttgttga tattccatag
     2821 gcatcaaaaa tgactggaac tatctttttg ttcttatcga gtgtcttttt ggcgtttaaa
     2881 agaattgatg acttgccagt tctgcggagc ccaagtaaaa tgacgttgtt gatatccccc
     2941 tttggtgtgc ctgaaagcaa ggctaaaagc tgctttaatt ctgatttcct gtcgataaag
     3001 tattttccag ttactggctt gcctacaaca aagggttgtt ccatggtaat ataggcaagc
     3061 ccgcatatat atgcattttt gcatctttta ccatgatgtt tgtaatgatg ctcattggaa
     3121 ttttaatcga cggcctagtc ttcaaaagac tagaagacaa ggtcatgcca agatggggcc
     3181 tgtgatgaga tcgtttgaac tcgctcgtta attcatataa gatttcttat atgttagaaa
     3241 tcttatacgg ctcggtatta ccaaaatccc aaataatggg taaaaccatc ttcgataatg
     3301 cgtgtgcaac atatatagtg aaaaactata atattatagt aatttactat aatatgcaag
     3361 aaaatggacg aaacaacata cagaatactt gatgtgctct caaggaatct aggcaaacca
     3421 atatcgatac atgaattgac tagcaaaatc caagaagcgt acggggctgc gtattatgct
     3481 aacatacatg aaaagattgg ccagctgtcc agggaacgca tgatcgagct tttgagggcg
     3541 ggaagatctt ctctggtctt ccttaacttt gagaactatc tgctaattga tttgcttgcg
     3601 gaaatggagc ttaagaggaa acgagacttt cttagtggga aaaaagagat gcagatgctc
     3661 atgatggaaa tggatacact tgtgcacaat atgcctttta tcaagtcagt caccctgatg
     3721 agcccacaaa gaaatgcaaa acttaacaaa gccgagctgt tgttttgcat aaacaagtcc
     3781 gacagaaaat caacggaaga aacaaaaatc agcatcaaca gaatcacagc taatctccaa
     3841 aaggcataca acattagaat agaccatctt attttagaag acaaagattt tgttgatctg
     3901 ctaagatcca atgagaacaa tcctgccaaa gagatgctgc acaacaagat atctgctctg
     3961 catcctcaga atttctggat gtcaataagt ggtgccatca ggggtggaat aaagatagta
     4021 gcagaagaaa gccagacaaa tccagcaaag ataacagaag aggatcttgt tttcaacctc
     4081 gcaagatttg gatatgtcga attggggtca aaaataaaac aaggcaggct gatctgcata
     4141 ggatacatca tagctgccat aatgttcaaa aatgatgcca gaaggatgga cgcaatccca
     4201 gtaattcttg caaagaattc ggcaaactac aacttggtgc tctttttggc gcgtaaatac
     4261 ggctttgaag gaaaaatact gggcatcctt gcggcgttgc ggaatctaat tgggaataaa
     4321 atggccaata ttgacgagcc cgtgaggctt cttgaggcta tgaaaataaa ggaaatcaga
     4381 gcagacaaaa aaggaatcaa ggagaaactg aggctgtaca atgtcgcttg ataacacaaa
     4441 actgctggac tttcttggag aaattgacaa ggaaccagaa cacagaatcg tcatagttgc
     4501 ggttggtggg acggcgatga ctcttgaatg ccaaaccatc caccatagat gcatcaagag
     4561 cattgttcaa gcctcggtgt attggttaat tttaaatact tactagatta ccaatatatt
     4621 ggtaaatcgt tatgaatata actgatacca atatattggc aaagtcaccc ccaaaacgtg
     4681 gtcaggtaaa agaaaggata ctacgagtca ttctcagtca cacaaacgag aaactaacca
     4741 aatatagaat aatgaaactg gccaaggcaa atatttcttg ggtaatagaa ttcctcagag
     4801 ttttacaaag taagggacta gtagaaggaa cttcaatcaa agactacaag ggtctaattg
     4861 tgtattggaa aaaaatgagg attaaaccag aatcgcgaga atacctgatt caacaacccc
     4921 tcgaagtgtt aagaaatgca aacctgcaat acgcgctaac tacatactat gcagaaaatt
     4981 tggtacaaaa gtatcttttt acgtcaagga ttgatgttta tatctcaaaa aatgattttg
     5041 agatctggca caaactcttg tctaaatccg gcttggtagg gaagggcaac gtcagagttc
     5101 tagttgatga tgagcatgtt ttttacaaat catctgaaag ggcaggatta aaaattgtat
     5161 ctatgccaca gctgatagtg gacctactag tggagggggg gccatgcgag gaggctgcca
     5221 acatgcttat ccaaaaggag atcgacaatg tttcaaaacc atgagctaga tatttccaag
     5281 tcgaatttaa ctacagtatt ctcaaagatg agggagcccg tatgcctact tggcggatgg
     5341 gcagtccacc tgacagtcaa tcaaaacttt aaagatgtca ctggcaggga ctacattggt
     5401 tccagagata ttgacttggg attccacatt gatccagatt ggtcaacaac agaactacag
     5461 aactctgcat ttgcacaatc tgtaaaaacc ctcgttgata acaaatttgt tggctgcggc
     5521 tcgcgattcg taaagcatta tgacatatct accctaaagg aactaacaga ggaccaaaca
     5581 aggaagaagc caatgtatga aatgtttcaa ttgtttgtgg atccaatagt agataacata
     5641 caccgggact ctcaaaaatt gggatttacg cctcttgacg agtctttact ttcacatgtt
     5701 ttcaatggca aaaaattcac ttctctgagt ggttttggcg gcaaattcat gctccccaca
     5761 tccgaaatac tgcttgccac aaagatatcc tctgtgccta aaagagacat ggtacacaag
     5821 aggataaagg acattgcaga tatctacgca ttatcgtggc attctggact caaatttgag
     5881 gatttgaagc aaaaactgca acaaattgtc gataaagtgg taatatctga cgtggtgtca
     5941 acattcacga aatcagacta tgatgctgtt ttcaatgcaa ttggagtaac gcaagaacaa
     6001 gtatcaagag ttataggcga gcttgcaaaa tagccaactc atttaagaat ttaacaattt
     6061 ttcctgattt tgtgccttta tcttcaatga caccaaaaac gcgacaatgc aaactgtgtt
     6121 tatatcgtag aaacaccatt gcacatatct tggcaagaaa aattatctct cttcaaccac
     6181 cttttagatt tatagaaaaa aacgaaagaa ttagaaactc acccaaatcc caatcagtac
     6241 ggcgaaccaa aaaagggaat ttggaagacc tgccgtgcag aacacattac aaaatcaatc
     6301 agctttgttt ttagatattt tccagaatca gatttacttc aagtgatgga tcttgacgat
     6361 cataaaacac tctttggtag agataattga aggtgttcag atgtcttgaa tctttgactt
     6421 tatcttttga attgcctcat ctggaatcac agtaatacca tggtcgtgtt ttgcatgatc
     6481 cgttatcttt gcaaacagtt ctgattcgtc atctgcagta gcagaccatg cacatccact
     6541 cataacatcg ccgcaggcga atttttttgc catggtattg tatggtcaat atgagattta
     6601 acaggtgcga tctaaaggca tgccgcaact ggatgggtat ttactaataa aaacgcactc
     6661 tactaaaccc aatgaacaca aaacaaatcg gaacattgac ggcaatcact gctgcggcac
     6721 tagtctttgc aaacataaac gtggcatttg cagattcgta caaaaactac gacaagcgtg
     6781 caatccacgc aatggaagta aagcaggccc aggcggcaag caagatggtc aagatattag
     6841 aggcatacaa gctgaccctg actgaacact cgtttgacaa caaggcaact gagaagagag
     6901 tggctggcaa cgcaaaatca attgataacc agataacagg tattgtggat ttgctaaaaa
     6961 acaggtcaca acctttagtc aaagcgctca ctgccccatc agctgagatt ctaaaaattg
     7021 ccaagtttgg ctcaatctca cagggcggat cagaggtaat gcgggttaca tacaaagtca
     7081 ctgcgggaaa ctatgatctt agggatgtca aagcagtcgt gggatcagag acag
//
LOCUS       DASWKH010000070        32195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3155028, whole genome shotgun sequence.
ACCESSION   DASWKH010000070 DASWKH010000000
VERSION     DASWKH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 32195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32195
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3155028"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VGA92_05065"
     CDS             <1..322
                     /locus_tag="VGA92_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9845818.1"
                     /translation="SILDSGWGIFANMLDYKTMLFDVPAKNTSIMCSRCGNAVPKSLA
                     IRTHRCDVCGLILDRDYNAAINILKKGLDIFNIKLPQELWEVTPVEITKWSMKQEEAT
                     GQVR"
     gene            409..1755
                     /locus_tag="VGA92_05070"
     CDS             409..1755
                     /locus_tag="VGA92_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9845819.1"
                     /translation="MSSEDIEKMIAQKKEKIGAGYLTDQGALFLVASDLGLTISEPVK
                     NEVSIKDLYSGAKEVTLQTRVLNVSPKKQFSRKDGSQFLLRNMTVYDNDSSVTVKLWD
                     EKANLPDTANIKPGDLIKIIKAYVKSDLNGQPTLNIGSGSTIEVADSDSNIPSIESLT
                     KDISDIKDKENNLVVSGVIDGNISYSEFTNIRGSQGSALRFRLKGKDGRSTRVVLWNK
                     NNSDIPKVIPAGTKVRMLGVRAKTNQQELEVHGNESTILEIDATQQVEPVVFRVLSSV
                     KNENGQRMILGVDKEKQLFNIVDTSDSTKSFGVNDVLECMPSKLYGNNVTIDAESFVR
                     KIDVEDLPNLPDLRTKIASIKADNDYYCIEAIILRAPEKRNVQTKTGETITLSEMFIE
                     DDTAQIWLKGWRNQARLLDDCSIGEIISVSGVVAKQGLEGRTELFLTPYSSIKKKN"
     gene            complement(1752..4499)
                     /locus_tag="VGA92_05075"
     CDS             complement(1752..4499)
                     /locus_tag="VGA92_05075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9845820.1"
                     /translation="MSALSQEKIHNKQLESVLAKFHSLGFTQLTEIQKKAIPTIIQKK
                     DSLIIAPTGSGKTECAVIPVFSFIASSKQKGKIKALYITPLRALNRDVFKRIIKYAEA
                     EGLAIEIRHGDTPQNIRKKITAQPPDILITTPETLVILLTQEKMLNALSELEWIIIDE
                     IHDLLSNERGSQLSLSVERLQINAKHPITRIGLSATVGNVDDAAKFLVGTKRKYKIIK
                     DNTIRKYDVEIKHVNGTIHDVADFIIDYLVKLNLSSPVLLFTNTRGEAEFLASILKEK
                     SPISVELHHGSLSQQAREETEENLKAGKSRLVVCTSSLELGLDIGSVELVMHYGSPRQ
                     VSKFMQRIGRSKHNRHSSAKGVIVTNNVDDELETKAILQRIQEGSIEDQAIHDGSLDV
                     LAHHLVGLSMQLGAISVSDAFKMFRDSFLFRNLTLEELIDVLELLDSNFLIRFDREKM
                     IFKKTAKCYRYYFENLSTIPDILKFKVFDTITKKMIGSLDQRFVGDFGEPGNVFVLRG
                     MQWRIINVDESALRVNVEPIRSGGITVPYWEGENIPVDYKTASKVGQLRTKIKNGLLN
                     LPENAIAQLSLPIIPDEKTLVVESVRGQGMIVIHSCLGTKINATLATILSSMLSSQTG
                     FQVDARSDAYRIALSSRGRIQENQVRQILTDSYDLYDVVTASLNGTHNVSWRTWNVAK
                     KFGVVGRGAIYDKKSARFLFERYSKTALVKEALRELFHDKYDLENSAKILDNIRANKI
                     QIQWVEENKFSKLAEPILDHTTKYYSSPANIDKGLLDLVKGRLFKTSHRLICARCGKW
                     EKVIQTNDIEKIPSCPHCKSRQIATTFYSDYDLAKIIQKKVAGKKISGEENHKFARAW
                     KTASLLANFGKTALIVLSGYGVGADTASRILRNMVDEDTLYRQIYEAERLYVTTRGFW
                     D"
     gene            complement(4599..5261)
                     /locus_tag="VGA92_05080"
     CDS             complement(4599..5261)
                     /locus_tag="VGA92_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9845821.1"
                     /translation="MIKTGLEKLDNILGGGIKSGIITDIFGASGTGKSQLALQIMVSS
                     LLEGGTTFYQDTTGNFRPERMLEMFKPRGLDPSLLDKITVGRITNTSEQLGNISKIYE
                     SNFSLVVVDNVSDLFSFEYSKEGQMLEKTNQFAQYMRELSKAALDKKIPIIIVNMIRK
                     MDEKEQENLESIVSLFTHTKIKLVKKSSNYEGQVILSPLKKNHFWYKITKEGLVELTE
                     AI"
     gene            5388..5630
                     /locus_tag="VGA92_05085"
     CDS             5388..5630
                     /locus_tag="VGA92_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0000398 - mRNA splicing, via spliceosome
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U6 snRNA-associated Sm-like protein LSm6"
                     /protein_id="HEX9845822.1"
                     /translation="MSQVSAKKPLTTLQKNTKKKVTVRLKNEVEYKGKMDNVDSYMNL
                     IMTDAEEMHDGKIIANYGRVIVRGNNVLFIRLENDL"
     gene            5643..6800
                     /gene="metK"
                     /locus_tag="VGA92_05090"
     CDS             5643..6800
                     /gene="metK"
                     /locus_tag="VGA92_05090"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX9845823.1"
                     /translation="MVRSFLFTSESVTEGHPDKICDKISDALLDEYLRQDPDSRVAVE
                     TMTTTGIVVVAGEVTSKAKFDIQDVVRNTIREIGYDKPEYGFDANSCSVLVSIHAQSP
                     DISQGVTANDNKDQGAGDQGLMFGYAVNESEELMPLPILLAHKLTRKLTEVRKNKELP
                     WVRPDGKSQVTVEYEDGKPKRVDAIVISTQHSPEIQNSEIRDKIIEKVIKPVCGKWWN
                     DKIKVHVNPTGRFVIGGPPGDSGLTGRKIIVDTYGGSGRHGGGAFSGKDPSKVDRSAC
                     YMCRYIAKNIVSAGLAEKCEVQVAYAIGVAEPVSLMVNTFGTGKMPEEDIENLVRKHF
                     DMRPAAIISHLGLKRPIYKDTAAYGHFGRTDVLFPWEKTDKADLLKKAAGL"
     gene            complement(6795..7643)
                     /locus_tag="VGA92_05095"
     CDS             complement(6795..7643)
                     /locus_tag="VGA92_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX9845824.1"
                     /translation="MDDLKIAITGPNGFVGSNLRRFLSNKKIYLISLSRKKYKPYKFE
                     KRILFSDLEQGGLEKKLEGCDVLVHLIGIGAQTADADYISVNVELTKKILKLCKKANI
                     RKIVYISGLGVNNSTTFGYFISKLRAEQQIINSGIDYTILRASYIIGKNDPLTKNLSK
                     QASRGSIMIPGSGKYRLQPISVNDVSRVILDCITNKKLSNKIIDLVGPQTISFEKFVK
                     RFVKGKKVKIIKTDLEKAYFEALNNPKNAIYGLDDLNLLVGDFTSSHKRLEMLCGFKL
                     RMLQKL"
     gene            7673..7891
                     /locus_tag="VGA92_05100"
                     /pseudo
     CDS             7673..7891
                     /locus_tag="VGA92_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            8180..9271
                     /locus_tag="VGA92_05105"
     CDS             8180..9271
                     /locus_tag="VGA92_05105"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX9845825.1"
                     /translation="MSETEKPREKLRTGFTTGAAATAASKASLLAIINQKKIESVEIS
                     LPKEKKITINLVSCDFDSSSAKCSVIKDGGDDPDVTHGAEIIVDLQLTNNVNQIEIDG
                     GEGVGRVTKPGLGLEIGSAAINPTPKKMITENLCQIGKQVLEKNGIKVIISVPKGKEI
                     AQKTDNPRLGILGGISILGTTGIVIPYSTASFAAAIRQSIDVTIAMKDNTVILTTGGR
                     SEEFSKKEISLPDHCFVQMGDFAGYAIEQCAKKGIKKVYVAGFIGKLTKIAMGVKQTH
                     VKGSKVDMDFLAKTASSCGASNTVLDQIKNANTARHVQEIVVKNKVEGFFSAVCSEAH
                     KQMRTHSDNKVEIEVILFDFDGSILGRYH"
     gene            9307..10362
                     /locus_tag="VGA92_05110"
     CDS             9307..10362
                     /locus_tag="VGA92_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX9845826.1"
                     /translation="MEKVAILAITKNGIKIASSIKQIFPNWVIFAPSKFQDDITNATW
                     YEEQTAAKIAELFGKFDGIVCLFSLGAVIRLISPYLKDKKTDPAIIVIDDKANFVISV
                     LSGHLGGANELTHEIAKKLGSTAVITTAADVNKTIAVDLLGREFGWKIEDDSNVTRIS
                     ALMVNEERIGVYQDSGEKNWWSGDLPKNVTIYDTPEKLMQADAKGVLLISDKDLDAGI
                     TKNAVVYRPQTLVAGIGLHTDTTKDKIKESLDSCLKKYRLSPKSIAKLVSLKKPTQIQ
                     GLVEAATDLGVPVEYFEREELAKINIPNPSDMVQAFEGTPSVSEAAALKASGGRLVIE
                     KQKFPPDLTIAIARIET"
     gene            10359..11111
                     /locus_tag="VGA92_05115"
     CDS             10359..11111
                     /locus_tag="VGA92_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX9845827.1"
                     /translation="MKRGLLLIDRGSREKEVKDELEFICNNVKQKGNYVFANYCFLEV
                     VPPFIEEGLKNCLTSEIDALTIVPYFLYPGKKVKAAVSEAVRFQAKTGIRFTVTKPMS
                     MHKTMIELVDSKIDAVLAKNNISLPKANIDVLVIGHGSKDPNARISIKYVVDNLAPKY
                     RNIDYCFLEIEEPNIYQGIEKCDKNNPELLVIVFYFLHEGAHVKRDIYEDLNPAVAKS
                     KIKKTLITEHIGTDNKMIDFIIERAQEVENAN"
     gene            11101..11733
                     /locus_tag="VGA92_05120"
     CDS             11101..11733
                     /locus_tag="VGA92_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX9845828.1"
                     /translation="MQTRKGQSIEDLSMKIIEKEIGTHPYSGHEWQIVRRIIHSTADF
                     DFARENQIIFHKDAVKNGLEALRAGKNIIIDVNGIVGLLNKQNLEDFKNNVMCDISNP
                     AVVKEASRLNKTRAQTAMRMRASEMNDGIVVIGNAPTALLEVIEMIKEKITTPKLVVG
                     IPVGFVAAAESKEALQAVDVPYITNRGRKGGTPCASAIINALFKILRESS"
     gene            complement(11725..13077)
                     /locus_tag="VGA92_05125"
     CDS             complement(11725..13077)
                     /locus_tag="VGA92_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HEX9845829.1"
                     /translation="MKIPRVVIAGATSGVGKTSITCSIIHAMRNRGYSIQPFKVGPDY
                     IDPTYLSAVSGNPARNLDSWIMGEDAVTRSFVTNSTSDISVIEGVMGYYDGFSGSSNL
                     SSTHHIATILQSPVILVLDASKTARSIAATALGYVKFHKNSRIVGLVLNKLGSKKHEH
                     MCRTALASLKIPIIGCVPKNPELSLESRHLGLIPIIEQDDLKHHVTKIAKSLAGYLDI
                     NKIVSIAKQSSTFIQIKGEKTEKAKVTIGVALDKSFNFYYYDNFDALRREGAKLEFFS
                     PISDSSPPDCSGLYIGGGFPEVLGQPLAKNHTMKKSIKKLAEDNMPIYGECGGLMYLT
                     KSIDYTSKKYKMVGLFDAETSMEKRMVLNYTKGNVVSNCLVAKKSAKLFGHEFHYSEL
                     KSVPKDSKFAYELSIGVGIKNKKDGLVEYNTLASYMHLYFDRSLHARNFITSCLKYSR
                     "
     gene            13152..13688
                     /gene="cobO"
                     /locus_tag="VGA92_05130"
     CDS             13152..13688
                     /gene="cobO"
                     /locus_tag="VGA92_05130"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9845830.1"
                     /translation="MEKDGGFVIVYTGKGKGKTTAALGMALRAVGHNYKTCMIQFIKG
                     SWHYGEMTSSKRLEPEFELTAVGKGFVGILDDKAPREAHQKIAQEAIEISKEKILSEK
                     YDIVILDEVNYAVNLGLIEVEQILDLIKIKPQNLTLVLTGNYAKQQVIDAADLVTEMK
                     EIKHPFQRGIRAKKGIDF"
     gene            13728..14528
                     /locus_tag="VGA92_05135"
     CDS             13728..14528
                     /locus_tag="VGA92_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9845831.1"
                     /translation="MATESQELPEIGEIVIATVAKLTDHGAYVTLDEYNSLQGFLHVS
                     EIAPGWVRSISKFVREGEKKVLLVKKVNPNRSEIDLSLKQVSKEQQKKKLLEAKRIEK
                     GKTLLQAVKESAVLTDKDLEKLEDTIFSKYDFVYDMFLDVVTKGITAIDELKLPKKTL
                     LAIEEVSAKIKPPSVEIRGICELTCNKSDGVDIIKNAILDSIGEHISNIEVTYIGAPK
                     YRISVKGQDFKTAERMLKPVLDKIQKIIEKNNGTFNFAREESKKTREG"
     gene            14531..14692
                     /locus_tag="VGA92_05140"
     CDS             14531..14692
                     /locus_tag="VGA92_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014121322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HEX9845832.1"
                     /translation="MKFQIRKCPDCKKYTLKDNCSQCNSKTITVHPAKFSPDDRYARY
                     RIKEKFKED"
     gene            complement(14689..17346)
                     /locus_tag="VGA92_05145"
     CDS             complement(14689..17346)
                     /locus_tag="VGA92_05145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9845833.1"
                     /translation="MISNKKLFSVGNFEFSLHHLLIIGILSLSFSISAMIRSQPAEYG
                     FQLNEFDPFFNYRATQFIVDNGLPAYFSWHDEMSWHPHGRDVASTSQAMLHITAAILY
                     GAFGGGSDLYGFTIIFPVVFGSLTAIVVFALVRVIGGTTAGLFASLLYAVSLPIIVRG
                     TIGWFKSEPLGLFYGLLAVYLFLSGLKSSSRKVAFLKLIGGGIFLGFAFSAWGGTEFF
                     VLPLGLFILSLPFVRKDGNFLRWAVPVFILGLAISLAPLERPGIAIFTHATGFMLIGP
                     TAFLVACTFIQQLSKEEHKKRNGGLFLIAIIVAGIGILSADVLGLPSFRYLNAINPFL
                     TTEDPLVDSVAEHSTTTIFQSFLFNSVFMIFGAIGAWLIFKNILNDSGKRDMHVFALI
                     IGLLGVYTSSAFVRLELFSSLSLVILGSVGLSLITKEIFRQDQQEKKKIIKSHPKTLK
                     ISYAAIIAILLIVPLTVPAGANWINSAQVPPTILNGGSNFNISTTDWLDAMAWLKENT
                     PEDAVVASWWDYGYWITTLGERKSLADNATLIDSKIKKIAQMFLSTPDEAWKILQELD
                     ADYVLVYIAAQRVNNEEPPLYIVQGGGDESKKQWFMRIGGFPVEKYLYQDGISPTAEF
                     WDETMLGKMIPYDTLAYVDLANQKQSNGFVPGFTPVYTNNTKFPQDGDGPLKLAYMSS
                     GFLRKDSGPINGVLIYEVNKDYNPQQNAVTTETTETTEATQDQIATISTTFGDIVIDL
                     KPEIAPKTVENFVKLADSNFYDGTLFHRIIPDFMIQGGDPNTISGPPGTWGSGGPGYS
                     IPAEISDLKHTKYVVSMARGQDIDSAGSQFFIMVGEASWLDGQYTIFGEVIEGQDVVD
                     QIAGLQTNLNDQPMDWESARIKTITISTP"
     gene            complement(17375..18031)
                     /locus_tag="VGA92_05150"
     CDS             complement(17375..18031)
                     /locus_tag="VGA92_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9845834.1"
                     /translation="MIEYNGVQIRWYGHDTFTLTHSNLTVCFDPYQLDKKISADIVLI
                     SHNHFDHMSNDDLKKITGDNTIIVAAKECLGQIKAKNKEIIGMLPNQEKAINGIKIKT
                     VPAYNIDKINPETKKPFHPKEDNKVGFIVTINNTAFYHTGDSDNISEMSDIQPDVLFV
                     PVSGTYVMTASEAASATQKIKPKLAIPMHYGTVVGSEEDAKTFKKLVTSCQVQILTKE
                     "
     gene            complement(18056..18433)
                     /locus_tag="VGA92_05155"
     CDS             complement(18056..18433)
                     /locus_tag="VGA92_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HEX9845835.1"
                     /translation="MVEIICDTSFLIHLATNRIKNLSSLEVEIGRVQFVVPDTVVAEL
                     TKLSQLDDKKTVALATLDFIKSYKIMKLGGSFADDIVVSHIKKHGGIVATMDRELKAA
                     IKKEGGSVISVANNKIILESSKV"
     gene            complement(18426..19688)
                     /locus_tag="VGA92_05160"
     CDS             complement(18426..19688)
                     /locus_tag="VGA92_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HEX9845836.1"
                     /translation="MHWRDTLPDWYVKKYGYQPCVNIGTAGHVDHGKTTLIQALTGQW
                     TSVHSQELKRGITIRVGYSDAAFYKCKNCEPPLGYSTIPKCPNCGKESELSRVVSFVD
                     SPGHESLMANMLSGAALMDGALLLVAANQKVPQPQTKEHLLALQILGIQQIVIVQNKV
                     DLVSYEDAMNNYSDIVKFVKGTKSVKAPIIPISAQSRLNIDALIGAIEESIPTPKRME
                     SKEPVMHVLRSFDVNKPGTKIKDIKGGVIGGSLTQGTFSIGDEIEIKPGILSSKKNNY
                     DPIRTQIVSLGTAAGIVDNVKPGGLVAIGTKLDPSLTRSDSFVGSVIGKPETLPENSI
                     TAQLEVNLFDSAVGTIHDVKVIPIQMGESLRLNIGTAPILAKVSKAKSGKIEVQFKRP
                     VCLFENSNVAISRKIEERWRLIGAGVIG"
     gene            complement(19720..20097)
                     /locus_tag="VGA92_05165"
     CDS             complement(19720..20097)
                     /locus_tag="VGA92_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6e family ribosomal protein"
                     /protein_id="HEX9845837.1"
                     /translation="MANFKLTISDKKGKSLSKELKDREANSLIGLHIGNELDAVVVGL
                     AGKIKITGGSDKSGMPMRSDIYGSARKPVLLSKGVGLQNIEKGQRRRRLIRGNTISEE
                     IYQINCSYDGELKIEEAPAENKA"
     gene            20175..20297
                     /locus_tag="VGA92_05170"
     CDS             20175..20297
                     /locus_tag="VGA92_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845838.1"
                     /translation="MKEEITCPRCNKKMVDMQACHMICDNCGAHLDCSDKGSFW"
     gene            complement(20299..20472)
                     /locus_tag="VGA92_05175"
     CDS             complement(20299..20472)
                     /locus_tag="VGA92_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-domain-containing protein"
                     /protein_id="HEX9845839.1"
                     /translation="MDAKCPECEGVAVLDDDMKSVKCPHCNFETDYENYLEIMKDKAI
                     NMASDYIPKRPGF"
     gene            20670..21080
                     /locus_tag="VGA92_05180"
     CDS             20670..21080
                     /locus_tag="VGA92_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845840.1"
                     /translation="MKRLETQHVEDIISWNKKETNERKESFGVKLEVLNGIVDKVNLL
                     SDILDDKERVLRQMSTLIGLIVFEQPFNNGNKATATSSAIQFLRTDGYELDLESEKAQ
                     DELLQMLDSIMYLFEDQSEQGIIAVRKFLEKHLK"
     gene            21292..21365
                     /locus_tag="VGA92_05185"
     tRNA            21292..21365
                     /locus_tag="VGA92_05185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21326..21328,aa:Gly,seq:ccc)
     gene            complement(21367..22308)
                     /locus_tag="VGA92_05190"
     CDS             complement(21367..22308)
                     /locus_tag="VGA92_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9845841.1"
                     /translation="MSEHELLLPPVTEENICLPLAVSAVSKYWDVNLPVAEAKEIAKK
                     YPNVRGSILIEGMELAERHGLGSLILHSSLSELKKIIDMGIPPIVILPGLFEIVQHAS
                     VISGYDEKEKSIIHYMPQPDQIGVIPEKQFDRLWEEDGRLLILVAPTDIISTIRVENR
                     SKEKSNRLCFVSEKLNLQNKTDEAINALNDAISLDGTNSTALCLIGGIYNEKNSPDCV
                     NFYEQSIGHNKKCFLAYRGLGNYYLKTKQYEKAISYYSQAIDINQTRFGPIYKNRGIA
                     LLELKKNKEAKQDFENYLKFAPNAKDKENILQAIKEL"
     gene            complement(22341..22886)
                     /locus_tag="VGA92_05195"
     CDS             complement(22341..22886)
                     /locus_tag="VGA92_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962336.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9845842.1"
                     /translation="MLLVVDNGSVFTKNIVQFLLEKKSIDFAILPFDKVILSELSKFQ
                     SFILSGRRQNNKEMNAVNSKIINHSISEKKSLLGICYGAEILALTVGGTIRKMSTTQK
                     GTNQVTVVAENPLCSGMIQAYQSHNYEISRLGKSLIQLAKSDTCNYEIIQYQNLTIFG
                     TQFHPEMSKDGLSVIERFTKF"
     gene            complement(22957..25803)
                     /locus_tag="VGA92_05200"
     CDS             complement(22957..25803)
                     /locus_tag="VGA92_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX9845843.1"
                     /translation="MYNSSSETNAPQPDAGKYIRLGIIAVIGIIVLTIVGNQGVILSM
                     NMTEFGEQFTKPLYYSLISAVVLSAIALVNVDIKNRSSILWYAIHVAITFLNRTIHDP
                     VSKNIPSFRDYKLSVPHFVIWQITKVFLFGAFFVNIMFGVAFVYMLEGNDLGINNLPN
                     LFALPFSTPFSTDAAQTVITLIPALTILVPPLLGVIGMRLAIYVGLHHIVKVITSYIH
                     DSSQGKPKFLNYISTIEAIIGIGIIWAGINMFFTEHIDYNTRYVIGGTLAAGFALIAF
                     SVLDKIRSKILTHPIKRDIYIRVFTLIAIGIIAGSVMAVNNSIADTRKLEYLGPYTLQ
                     QIEVNRYLGELDKIKTTTNEVRLTSISPNNIRSFMQQNSDVLDSIRIWDWEAASAKLR
                     PEIGLIPYIEFQDNDVLRFNSTLYWTVSMKPVLPSSVSLENRWYNEHLVYTHVPNGFL
                     TLDATTGQTIDSSNLFSQRAIYYGESGLFEQTWSAYPTTRGSVSAELNNAFYTGSGGV
                     QISPPLSWIFEPNFLLSYPATPIHTLRYKDIHDRMELLYPYFLYSLFGQQLDVLPVTD
                     GTNTYWLVPLIIGFDTHSVPWAMGNPYLRLVGFALIDTYNGDITLLEYGDSYFTKILE
                     SQYGDKFEPIPSWLNEQIRYPEELFTWKTEMFNIYHVTDIETFIQANEFYEIPATLEA
                     YYIEAKPPGYESTKFLGLLSLELRGSQGKNLSGYMIVENDMPALGQMRFYEVPLNSTT
                     KLIGPTAVREALDKDSDFAQLKTLLRTPRIGDNILYRVGDHDVYFIPVYTAGSGGGVV
                     AQLGTIAAVGAAFNGEYYVGLGNTQHQAFEAYLRELAGVVVPSTPTGEVGFDKNARIQ
                     QIKSVFEENDIEIVTPTSLQIPISFKEGEISLFSHSDLDATNEMLNKFIDDFVLPSTK
                     RVLWWEVDQAVNIGTITIVDGIPELHYVSIEVGK"
     gene            complement(25861..26982)
                     /locus_tag="VGA92_05205"
     CDS             complement(25861..26982)
                     /locus_tag="VGA92_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEX9845844.1"
                     /translation="MLEKEIKLVEESFKKYYFDHFDLIHTPANPEEREFGYQKFNSGM
                     NRHISLKSDKELHLLLMTQVPSDVYCSNARYSFPNLPMAEKDWQKADLIFDIDAKDLN
                     LPCRTNHICKKCSNCANVFANQEQCPQCNSAKFDTVSVLCKDCILAAKNEVKKLISIL
                     ESDLGIKNENIQTYFSGNEGFHVHIPNSSYEKLESKERAELVDYLMFRGAMPETFGAK
                     LSNFAKSTFAEPDDKGWLGRVSKQIFGSKSNKAKASKQIISEGYMTFKRKLEELQRQI
                     GVQIDPSVTIDVHRIFRLGGTINSKSGLVKILCEDISKFNPSNDACFIGDDNVTVLAD
                     CPVEFRLKGKKFGPYKKEQVTIPRHAAVYMICKGCATLA"
     gene            complement(26975..27991)
                     /locus_tag="VGA92_05210"
     CDS             complement(26975..27991)
                     /locus_tag="VGA92_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9845845.1"
                     /translation="MLKLGTEEIAKYPFLTEAGNYLREKGFTLEQFDKDEDLRPIIGL
                     ALSRIETAANGKIFNSDFSIKNLDIEVFSFLIAVILLKQSGMNTLIKRFSLAEARRAE
                     KFLEKDLADINNTTKEQLAIKIIRDLFAMEIQKHDDYFVIPVPDYLKHATHFHEKEWK
                     LVNRLVDNGNVFLSAHETVRLIRKELDNFISSKIQSASIPLVPDSFKKPVSDLVDLAK
                     KFTVQFVETTEYPPCIKHAIEVLSKGENLPHSGRFMLATYLLNKGQTIEDIAPLFKNA
                     PDYNEKVTLYQLKHLAGNFGSGTKYSCPSCDKLKSENLCYAIPECDNIINPLQFGRKK
                     ITNA"
     gene            28072..28911
                     /locus_tag="VGA92_05215"
     CDS             28072..28911
                     /locus_tag="VGA92_05215"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX9845846.1"
                     /translation="MRVGLHVSISGSLAAAVDNAVERKCSAFQIFTRSPRMWAAKDIS
                     EKDASDFRERLASSKIDRLATVAHMPYLPNLASPNAATHAKSVDVLIKEVKRCAQLGI
                     PYLVAHLGSHMGEGDEKGIKQLVSAFSKAASVKNDVVILLENTAGQKNSVGSEFDQWA
                     KIFFKLKPKERFGVCFDTCHAFAYGYDLRTEKDVTNTFKKFEDAIGFEHLKILHLNDS
                     KGEIGSNLDRHEHIGLGKIGEKGMGVIVRLANKKDIPIILETPIDNTRDDFGNIKKVK
                     ELA"
     gene            28949..30406
                     /locus_tag="VGA92_05220"
     CDS             28949..30406
                     /locus_tag="VGA92_05220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX9845847.1"
                     /translation="MNYDSIMQLALERGFYFPSCEIYSDAQAGFWEYGPSGVSMKTKF
                     IELWRRELVRRDGMLEIDGSQIMSQSVFEASGHLASFADPIVRCKQCGLNFRADRLIF
                     EQTKIEIPESADLGDFDKTIMEKNIKCPKCKGEFDKTRKFNMMFKVEIGPEAEPAYLR
                     PETCQSIFVDFPRLFKTMRGKLPLGIAQIGKSFRNEISPRQSLLRLREFYQAEIEVFC
                     NPNKLNDLERFSEVQNTIIRLWINDESKAMSCKQAVDSGILPNKFVAYYLGILTEFYE
                     KTGIDMTKSRFRLLGEKEKAFYATVAFDFEVQTAIGWLELVACNYRSDYDLSSHANKS
                     KEKFEVLDDEQKVLPHVFEISMGIDRSLYTILEHGLKEDKQNDRIVLSIKPYLAPIHI
                     GVLSLVKKDGLKEKTDEIYLKIKRKYDAFLDHSGAIGRRYRRLDEVGAPFAITVDHQT
                     LQDDTVTLRHRDSMQQDRIKVSDIDSVLSNAISFP"
     gene            complement(30468..>32195)
                     /locus_tag="VGA92_05225"
     CDS             complement(30468..>32195)
                     /locus_tag="VGA92_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845848.1"
                     /translation="PSVSDEPTNTAFIGAGGNCKITSGSDLYVWTRHFTSFASYTSSA
                     TPSAPSPSTPAASGGGGGGGGGGAAAGATGFGGKLVQPIVIYEISYDLCEQNMIRIIV
                     GIQGLETPPPNVKIRTPTQKIYSATLVQDQPYAEANKAFSTSRYVYEAPLDPKLDFFI
                     VTAEQIAGRDVVIARYLVNVDECRNVIVVNSMEDIERTETSETTVGVKRPQIFDIKFQ
                     VDGNKPIKATEINQFVEPGAQLKFSAIVDSPSTIRRAELRVSTAGGNYGDYAAVVMKI
                     APLQNITNAYLVSVDLPSLFLQTPATVYWIYVLNNDGQAQSSERYILGVKPPYEFDAR
                     IMLDSPPSKAESTMYRPTAYVNNDDEKPLFGTASLLVNGTIVYTSPEQLFNKGESVVN
                     FEWKIPNVRAESKYQVSAQLNLYDKTIETTSLILRTFQTTRIFSISEPVTINSVMENG
                     DVVARAGLLYSSDTNPSLHYRIVAPDGTCIIGKSDPCLVSGSTAGFRGNSVNVDLDGQ
                     IYRIRYSGQDSPLERFSITSIDPIVGNWIVTLESDDGVIPEAHALEDVRLKVKYRANY
                     IKLITTTSD"
BASE COUNT         9449 a         6268 c         6679 g         9799 t
ORIGIN      
        1 aagcattctg gattctggtt ggggaatatt tgcaaacatg ctggactaca aaacaatgct
       61 gttcgatgtt ccggcaaaaa acacatccat aatgtgttca aggtgcggca atgcagtgcc
      121 aaaatctttg gccatcagga ctcacagatg cgatgtatgt ggtctgattt tggataggga
      181 ttacaacgcg gcaattaata ttctcaaaaa aggattagat attttcaaca ttaaactacc
      241 gcaggaactg tgggaagtaa cgcctgtaga gatcacaaag tggtctatga agcaggaaga
      301 ggctaccgga caagtccggt agtagttcac taggatatga tataatcaat gatcaacttg
      361 tctgaatttg atgccctgct cagtaaactc cttgaacaga gacccgaatt gtcaagtgaa
      421 gacattgaga agatgatagc gcaaaaaaag gaaaagattg gggcaggcta tcttaccgat
      481 cagggtgctt tgtttttggt cgcatcagat cttggattaa caatatcaga gcccgttaaa
      541 aacgaggtca gcatcaagga cctgtactca ggagcaaaag aggttacact acagacaagg
      601 gttcttaacg tttcaccaaa aaaacagttt tcaagaaagg acggctcgca gttccttttg
      661 cgaaacatga ctgtttatga taacgattca tcggtaaccg taaagctttg ggatgagaag
      721 gcaaacctac cagataccgc aaatatcaag ccgggcgatt tgataaaaat aatcaaggca
      781 tatgtgaaat cggatcttaa tggtcagccc acactcaaca taggctcagg ctcgacaata
      841 gaggttgctg actctgatag caacattcca tccatagagt ctttgacaaa agacattagc
      901 gacataaagg acaaagaaaa caatcttgta gtttctggag taattgacgg aaacataagt
      961 tattcagaat ttacaaacat tcgtggaagt cagggtagtg ctttgcgatt cagactaaaa
     1021 ggcaaagatg gtcgttctac tagagttgtg ctgtggaata aaaacaactc tgatattcca
     1081 aaagtaatac ctgctggaac caaggtcaga atgcttggag taagagcaaa aacaaatcag
     1141 caagaactcg aagtacatgg aaacgagtca accattcttg aaattgatgc aacgcaacaa
     1201 gtcgaaccag ttgtgttcag agtattatca tctgttaaaa atgaaaacgg gcagagaatg
     1261 attttaggtg ttgacaaaga aaaacaacta ttcaacatag ttgatacttc cgattcaacc
     1321 aagtcatttg gtgtaaatga tgttcttgag tgcatgccat caaagttgta tggaaataat
     1381 gttacaattg acgcagagtc gtttgtgcga aaaatcgatg tggaggatct acctaacttg
     1441 ccagacctca gaacaaagat tgcaagcatc aaagccgaca acgactatta ttgtattgag
     1501 gcaataatac ttcgggcccc agaaaaacgt aatgtgcaaa caaaaactgg ggaaaccatc
     1561 acactttctg aaatgttcat agaagacgac actgcccaaa tctggctaaa gggttggcga
     1621 aaccaagcaa gattattaga tgactgctca attggcgaaa tcatctctgt gagtggcgtt
     1681 gtagcaaagc agggcctaga aggcagaaca gagttgtttt tgaccccata ttccagcatc
     1741 aaaaagaaaa actagtccca aaagccgcgc gtagttacat acagcctttc ggcttcgtag
     1801 atctgccggt ataacgtatc ttcgtctacc atgttcctta gtattcttga ggcagtgtct
     1861 gcgccaaccc catacccaga taggacgatt aaggcagtct tgccaaaatt tgccaatagg
     1921 gaggcagttt tccaagcgcg tgcaaacttg tggttttctt cacctgaaat ctttttgcct
     1981 gcaacttttt tttgtatgat tttagccaga tcgtaatcgg aataaaaggt cgtagctatc
     2041 tgtctgctct tgcagtgagg acaacttggt attttttcta tgtcatttgt ttgtattacc
     2101 ttttcccatt ttccacatct tgcgcaaatt aacctgtgtg atgtcttgaa cagcctcccc
     2161 tttacaagat ctaagaggcc tttgtcgata ttagcaggag acgagtaata cttggttgtg
     2221 tgatcaagta ttggctcagc taacttggaa aacttgtttt cttcaaccca ctgaatttgg
     2281 attttatttg cacgaatatt gtctagaatt tttgcagaat tttctaaatc gtatttgtca
     2341 tgaaagagtt ctcgtagtgc ttctttgacg agtgcagtct ttgaataccg ctcaaagaga
     2401 aatcttgcag attttttatc atatatggca cccctaccaa caacgccaaa tttttttgcc
     2461 acattccagg ttctccaact tacattatgt gtgccgttta gcgaggcagt tactacatcg
     2521 tacagatcat agctgtcagt taagatttga cgtacttggt tttcttgtat tcgtcccctc
     2581 gacgacagtg cgatcctgta tgcgtcggat ctcgcatcaa cttggaatcc agtctgtgat
     2641 gataacatgg aggaaagaat tgtagccagt gttgcgttaa ttttagtgcc aaggcaagaa
     2701 tggataacaa tcatcccctg acctcttaca gattcaacaa ctagcgtttt ttcatcaggt
     2761 attattggaa ggcttagctg ggctattgca ttttctggga gatttagcag tccgttcttt
     2821 atctttgttc tcaattgtcc tacctttgag gcagtcttgt agtctactgg tatgttttct
     2881 ccttcccaat atggcaccgt tatgccgcca gaacgaattg gctcgacatt taccctaagt
     2941 gccgactcgt ccacgttgat tattcgccat tgcattccac gcaaaacaaa aacatttcca
     3001 ggctcgccaa aatccccaac aaacctttgg tctaggctac caatcatttt ttttgtaatg
     3061 gtatcaaaga ctttgaattt caaaatgtca ggaattgttg aaaggttttc aaagtaatac
     3121 ctataacatt tcgctgtctt tttgaagatc attttttccc tatcaaagcg gattagaaaa
     3181 ttagaatcaa gtagttccag cacgtcaata agctcttcaa gtgtcaaatt tcgaaaaaga
     3241 aacgagtccc taaacatctt aaatgcatca cttactgata tagcgccaag ctgcattgat
     3301 aatccaacaa gatggtgcgc caaaacatca agtgatccgt catgaatggc ctgatcctca
     3361 attgagcctt cctggattct ttgcaaaatt gcctttgtct caagctcatc atctacattg
     3421 tttgttacaa ttactccctt tgcagacgaa tgcctgttgt gtttgctgcg tcctattctt
     3481 tgcatgaatt tggagacttg tcttggcgag ccgtaatgca tgactagctc tacagagcca
     3541 atgtccaagc cgagctcaag tgacgacgta catacaacca gccttgattt tccagccttg
     3601 aggttttctt cggtttcttc gcgcgcttgt tgcgaaagcg atccatgatg aagctcaact
     3661 gagatcggag acttttcttt tagaattgag gccagaaatt ctgcttcccc cctcgtattt
     3721 gtaaaaagaa gcactggtga gcttaggttt agcttaacta ggtaatcaat tatgaaatct
     3781 gccacgtcgt gaatggttcc atttacgtgt tttatttcaa cgtcgtattt tcttattgtg
     3841 ttgtccttga tgattttgta ttttcttttt gtaccaacga ggaattttgc cgcatcatcc
     3901 acattgccga ccgttgcaga aagcccaatt cttgttattg ggtgctttgc attaatctga
     3961 agccgctcaa cgctcaacga cagctgagag cccctttcat ttgataacaa atcatgtatt
     4021 tcgtcaatta tgatccactc cagctcagat aatgcgttga gcatcttttc ctgtgttaac
     4081 aaaattacta gtgtttcagg cgttgtgatt agaatatccg ggggttgggc tgtgatcttt
     4141 tttctaatat tctgaggcgt gtccccatgt cgaatttcaa ttgcaaggcc ttctgcctct
     4201 gcatatttga tgatgcgttt gaaaacatcg cggtttagtg ccctgagcgg ggttatgtac
     4261 agtgccttga ttttcccttt ctgcttcgag cttgcaataa atgaaaagac tggaattacg
     4321 gcgcattctg tttttcctga accagttggt gcaatgatta atgagtcctt tttttgaatt
     4381 attgttggaa tggccttttt ttgaatttct gttagttgtg taaaaccaag agaatggaat
     4441 ttggctaaaa ccgattcaag ttgcttgttg tgtatctttt cttgacttag cgcgctcata
     4501 gaccattata ccaatgattc caattgcaaa caaaattacc gaaatggttg gaattgaatc
     4561 aaaaatgcct gccatgacga gttagaccat ttccgtagtt aaatagcttc agtcagttca
     4621 actagacctt cttttgttat tttgtaccag aagtgatttt ttttcaacgg cgacaaaatt
     4681 acttgtccct catagtttga cgatttcttt acaagtttga ttttggtgtg agtaaacaaa
     4741 ctcacaattg actctaaatt ttcttgttct ttttcatcca tttttcgtat catatttact
     4801 atgattattg gaattttttt gtcaagtgct gctttggata attccctcat gtattgtgca
     4861 aactggttgg ttttttccag catttgccct tcctttgagt attcaaagga aaacaggtca
     4921 gacacattat caactactac caaagaaaag ttcgattcgt agatttttga aatattgcct
     4981 agctgttctg acgtgttagt tattcttcca accgttatct tgtcaagaag acttggatcg
     5041 agcccccttg gtttgaacat ttccaacatt ctttcaggtc taaaatttcc cgtagtgtct
     5101 tgataaaatg tagtgccccc ttccaaaaga gagctgacca ttatctgtag tgctagctgt
     5161 gattttcctg ttccacttgc accaaaaatg tcggtaatta taccgctttt aattccacct
     5221 ccaagaatat tatctaattt ttcaagtcca gtctttatca tatgacttct ttaaaaaact
     5281 agaaaaataa acaaatctca ccttaaaatt acaaggtagg caaggttaga tcttacagaa
     5341 cccaaggctt ttattcaggt tatcaagaag cttgacaaga gtaatcattg tcacaagtaa
     5401 gcgccaaaaa acctttaaca acactacaaa aaaataccaa aaagaaagtc actgtaagac
     5461 taaagaatga agttgaatac aaaggtaaaa tggacaatgt cgattcctac atgaatctga
     5521 ttatgactga tgccgaagaa atgcacgacg gtaagattat tgccaattat ggtagggtaa
     5581 tagttagagg aaataacgta ttatttatca gactagaaaa cgacctgtag gcaaagatta
     5641 caatggtacg aagttttctt tttacttctg aatcagtaac agaaggacac ccagacaaga
     5701 tttgtgataa aatttcagat gctctattag atgagtatct aagacaagac ccagattcgc
     5761 gtgttgccgt agaaaccatg actactacag gcattgtggt tgtcgcagga gaggtcacct
     5821 caaaggccaa gttcgacatt caggacgtag taagaaacac cataagagag atagggtatg
     5881 acaagccaga atatggcttt gatgcaaatt cctgcagtgt tttagtatca atccacgcgc
     5941 aaagcccaga tatttcacag ggtgttactg ctaatgacaa caaggatcaa ggtgcaggcg
     6001 atcaggggct aatgtttggt tatgctgtaa atgaatcaga ggagcttatg ccgcttccca
     6061 tactattagc acacaagctt acaagaaaac taacggaagt aagaaaaaat aaagaacttc
     6121 catgggtaag gcctgacggc aaatctcaag ttaccgtaga atatgaggac ggcaaaccaa
     6181 aaagagttga cgccatagtt atttcaacac agcattctcc agaaattcaa aattcagaga
     6241 tacgagataa gataattgaa aaggtgatca agccagtctg tggcaaatgg tggaatgata
     6301 agatcaaagt ccatgtaaat cctaccggcc gctttgtaat aggtggccca cccggagatt
     6361 cagggcttac tggaagaaag ataattgttg atacctatgg aggctcagga agacatggag
     6421 gcggagcgtt ttctggcaag gatccatcaa aggtggacag atcagcatgc tacatgtgta
     6481 gatacattgc aaaaaacata gtgtctgcag gactggccga aaaatgtgaa gtccaagttg
     6541 cttatgcaat cggagttgca gaaccagtct cattaatggt aaacacattt ggcacaggaa
     6601 aaatgccaga agaagacatt gaaaatctgg taagaaagca ctttgatatg cgtcctgcag
     6661 caatcatctc gcatcttggt cttaagaggc caatttacaa agatactgca gcctatggac
     6721 attttggcag aaccgatgtc ttgttcccat gggagaaaac tgacaaggca gatttgctca
     6781 aaaaggccgc aggcctatag tttttgcagc attcttagct taaagccaca caacatttcc
     6841 agccttttgt gacttgaggt aaaatccccc actaagagat tcaagtcatc tagcccataa
     6901 attgcgtttt ttgggttgtt tagtgcctca aaatacgcct tttccaaatc tgtttttatt
     6961 atcttgactt tttttccctt gacaaatctt ttgacaaatt tttcaaagct gatcgtttgt
     7021 ggaccgacaa gatcaattat tttgtttgaa agttttttgt tagttatgca atcaagaata
     7081 actcttgaga cgtcattgac tgagattggc tgcagccgat atttgccaga acctggtatc
     7141 attattgaac cgcgactagc ttgtttgctc aggttttttg tgagtggatc gttttttcca
     7201 ataatgtatg aggccctaag aattgtgtag tcaataccag agttaatgat ttgttgttct
     7261 gcccttagct ttgaaataaa atatccaaaa gtagtcgagt tattgacccc aagaccacta
     7321 atgtaaacaa tttttctaat gtttgctttt ttgcatagtt tgaggatttt ttttgttagt
     7381 tctacattta cagaaatgta atcagcatca gcggtttgcg ctcctatacc tatgagatga
     7441 actagtacat cacagccttc taattttttt tcaagccctc cttgttctaa atctgaaaag
     7501 agtattcttt tttcaaattt gtatggtttg tatttttttc tagataggct gatcaggtag
     7561 attttcttgt tgcttagaaa tcgtctaaga tttgagccaa caaatccatt agggcccgta
     7621 atagctatct taagatcatc cattcaatta agaagattag atataatttt aaatgtataa
     7681 ttgtgataga ttagatttgt tgaacgcatc gtattgttat gaggtgcttg gattacacag
     7741 agatgcatcg tttagagagg taaaacaggc atacagaaaa ttgtctttaa agtatcatcc
     7801 tgaccgaaac aaggatatgg gtgcagacaa aaaattcaaa gaaattacag aggcgtacca
     7861 gctgctaaag gttgaacaaa aaaaggaaaa cgcaaggcac aaagatgtag aagcggcaca
     7921 tacagagttt tggaattact acgacaaaaa ggcaagcgag gaatttggtt atagttatca
     7981 ggcttacttt aaggatctta ggcgtgattt tggaacaaac atggatgagt atagccacaa
     8041 tgtggaaaag ccagtctcgc acaaaatgac acatttgttg ctttatggtg gtcttggcgc
     8101 attagccctg tggataatca tttctgagat tttgaagtga gtcgataagc attattacat
     8161 aaaaacagaa taccaagttg tgagcgagac agaaaaacca agagaaaagc ttcgaactgg
     8221 ctttacaact ggcgctgctg caacagcagc atcaaaggca tcacttcttg ccataattaa
     8281 tcaaaaaaaa attgagtctg tcgaaatatc tttgcccaaa gaaaaaaaaa ttacgatcaa
     8341 tcttgttagt tgtgactttg attcttcatc tgcaaaatgt tctgtaatca aggatggtgg
     8401 ggatgatcct gatgtcactc atggcgcaga aatcatagtt gacttgcagc tgacaaataa
     8461 tgtaaatcaa atagaaatag atggtgggga gggagtaggt agggtaacaa aacctggcct
     8521 tggtttggag attggctctg ctgcaataaa cccaacacca aagaaaatga ttactgaaaa
     8581 tctttgtcaa attggcaagc aggttcttga aaaaaatggc atcaaggtga taatttccgt
     8641 gccaaaaggc aaagaaatag cgcaaaagac tgacaatcca aggcttggca tcttgggagg
     8701 aatatcaatt cttggaacaa cgggcatagt aattccatat tcaacagcat catttgctgc
     8761 tgcaattagg caaagcatcg atgtaacaat tgcaatgaaa gacaataccg taattcttac
     8821 taccggaggc agaagcgaag agttttcaaa aaaagagatc agtctaccgg atcattgttt
     8881 tgtacagatg ggcgactttg caggttatgc aattgaacaa tgtgcaaaaa aaggcatcaa
     8941 aaaggtctac gttgcaggat tcataggcaa acttacaaaa attgcaatgg gagtcaagca
     9001 gacccatgtc aagggctcca aagtagacat ggatttttta gccaaaacag caagtagttg
     9061 tggcgcaagc aacacagtac tagatcagat aaagaacgcc aatacggcaa ggcatgtaca
     9121 agaaattgtt gtcaaaaaca aagtggaggg attcttttcc gctgtctgct cagaggccca
     9181 caagcaaatg cgaacacatt ccgacaataa agtagagatc gaagtgattc tttttgattt
     9241 tgatggctca atacttggca gatatcatta gcaagaaatt ttatttgcct atagaatctt
     9301 cagaatatgg aaaaggttgc aattcttgca attacaaaaa atggcattaa aattgcatca
     9361 tcaataaaac agatttttcc aaattgggtg atctttgcac catccaagtt ccaagacgac
     9421 ataaccaatg caacttggta tgaggaacaa actgccgcta agattgccga actttttggt
     9481 aaattcgatg gaatcgtttg ccttttttca ttgggtgcag taattaggct aatttcaccg
     9541 tatttgaagg acaaaaaaac agatccggca ataattgtaa tagatgacaa ggcaaatttt
     9601 gtgattagtg tgttatctgg ccatcttggt ggcgcaaatg agctaacaca tgaaatagca
     9661 aaaaaacttg gctctactgc agtaattact actgctgcgg atgtcaacaa gacaattgca
     9721 gttgatttgc tgggtaggga gtttggctgg aaaatagaag atgattcaaa cgtgactagg
     9781 attagcgcat taatggtaaa cgaggaaagg attggagtgt atcaggattc gggagaaaaa
     9841 aactggtgga gtggagatct accaaaaaat gtaaccattt acgatacgcc agaaaaatta
     9901 atgcaggcag acgccaaagg cgttctgcta atttcagaca aagatcttga tgccgggatt
     9961 acaaaaaatg ccgtagttta cagaccacag acactggtag caggaatagg cctgcacaca
    10021 gatacaacaa aagacaagat taaagaaagt ctcgattctt gtcttaaaaa atacaggctt
    10081 agtccaaaat caattgcaaa acttgtttcg ctcaaaaaac cgacgcaaat tcaagggctt
    10141 gttgaggctg caacagatct tggcgttcct gtagaatatt ttgaaagaga agagttggca
    10201 aagattaaca tcccaaatcc ctccgacatg gttcaagcct ttgagggcac cccgagtgta
    10261 tctgaggctg ctgcactcaa agcctcaggt ggcagacttg tgattgaaaa gcagaaattc
    10321 ccacccgatc ttacaattgc catagcaaga attgagacat gaaacgcgga ttattattaa
    10381 ttgacagggg cagtagagaa aaggaagtaa aagacgaact agagttcatt tgcaacaatg
    10441 taaaacagaa aggaaactat gtttttgcaa actattgctt tttggaagtc gttccacctt
    10501 ttattgaaga gggactcaaa aattgcctaa cttcggaaat cgatgcgctc acaatcgttc
    10561 cttacttttt gtatcctggc aaaaaggtca aagcagcagt aagcgaggca gtcaggtttc
    10621 aggctaaaac tggcatcaga ttcacagtaa caaagccaat gagcatgcac aaaaccatga
    10681 ttgagcttgt agattccaaa atagatgcag tgcttgcaaa aaacaacatc tcattgccaa
    10741 aggcaaacat agatgtccta gtaataggcc acggcagcaa ggatcccaac gccagaattt
    10801 caataaaata cgtagttgat aatttagccc ctaaataccg aaatattgat tattgttttc
    10861 ttgaaatcga agagccaaac atttaccaag gaatagagaa atgcgacaaa aacaatcccg
    10921 aacttttagt catagtattt tattttctac atgaaggagc ccatgtaaaa agagacattt
    10981 acgaggatct caatcctgca gttgcaaaat caaaaatcaa aaagactttg attacagaac
    11041 atattggcac agataacaaa atgattgatt ttataataga gcgggctcag gaagttgaaa
    11101 atgcaaacta ggaaaggcca gtcaatagaa gatctgagta tgaaaattat cgaaaaggag
    11161 ataggtacac acccatattc tggtcatgag tggcagatcg taagaagaat cattcattcc
    11221 actgccgatt ttgattttgc aagagaaaac cagataattt ttcacaaaga tgcagtaaaa
    11281 aacggccttg aggcattacg ggctggcaaa aacatcataa tagatgtaaa tggtattgtt
    11341 ggtttgctaa acaagcaaaa cttggaggat tttaaaaata atgtcatgtg tgatatctca
    11401 aatcctgcag ttgtaaaaga ggcatccagg ctcaacaaaa cacgcgccca gacagcaatg
    11461 cgaatgagag catctgagat gaatgatggg atagtcgtaa taggaaatgc gcctaccgca
    11521 ctcttagaag taattgagat gataaaagaa aagatcacta cgccaaaact cgtagttgga
    11581 atacctgtgg gttttgttgc agcagcagaa tccaaagaag cgttacaagc cgtagatgtg
    11641 ccatacataa caaacagggg acgaaagggt ggaactccct gcgcttcagc cataataaac
    11701 gcgcttttta aaatacttcg agaatcatct tgaatatttc aggcaacttg tgatgaaatt
    11761 tcttgcgtgc aaggaacgat caaagtagag atgcatgtat gatgcaaggg tgttgtattc
    11821 taccagtccg tcttttttgt ttttgattcc aacgccaatt gagagttcat aagcaaattt
    11881 ggaatctttt ggaactgatt tgagctcaga gtaatggaac tcatgaccaa acaactttgc
    11941 tgattttttt gcaacaagac aattggaaac aacgtttcct tttgtatagt ttagaaccat
    12001 tcgtttttcc atgctggttt ctgcatcgaa aaggccaacc attttgtatt ttttactcgt
    12061 ataatcaatt gattttgtaa ggtacatcaa tccaccacat tccccataga ttggcatgtt
    12121 atcttcagca agttttttga ttgatttttt catggtgtgg ttttttgcaa gaggctggcc
    12181 cagcacttct gggaacccac cacctatgta aaggccagaa cagtctggcg gtgaggaatc
    12241 tgaaatcggg ctaaagaact ccagttttgc accttcacgc cttagcgcat caaagttgtc
    12301 gtaataatag aaattaaagg acttgtctag tgctacgccg atagtaactt tggccttttc
    12361 agttttctcg ccttttattt gtataaaggt agaagattgt tttgcaattg agactatctt
    12421 gttaatatca agataaccag ctagtgattt tgcgattttt gttacgtggt gttttagatc
    12481 atcttgttct attattggta ttaaccctaa atgtcgtgat tccagtgaaa gttctggatt
    12541 ttttggcaca cagccaataa taggaatttt cagagaagcc agggccgttc tgcacatgtg
    12601 ttcatgtttt ttgcttccta gcttgttgag aactaatccc acgattcttg aatttttatg
    12661 aaattttaca tagcctagag ctgttgccgc aatagagcgt gcagttttgc ttgcatccaa
    12721 aaccaagatt accggtgatt gcagaattgt ggctatatgg tgcgtactag agagattgga
    12781 gcttccagaa aagccgtcgt aataacccat cacgccctca atgactgaaa tatcagatgt
    12841 ggaatttgtc acaaagctcc tagttacagc atcctctccc ataatccacg agtctagatt
    12901 tcttgccgga ttgccagata ctgctgaaag atatgttggg tcgatgtagt caggtcctac
    12961 cttgaatggt tggatcgaat aacccctgtt tctcattgca tgaattatag aacatgttat
    13021 ggatgttttt ccaacaccgc ttgttgcgcc agctataaca acccgcggta ttttcagctt
    13081 ttttgccatt agtctttgca aatcaataca gctataccaa tctttttaga gttcaacttt
    13141 gattttgcct tatggaaaaa gatggcggtt ttgtaatagt gtatacaggt aaaggaaagg
    13201 gtaagacgac tgctgcgttg ggcatggcac taagggcagt tgggcacaat tacaagactt
    13261 gcatgatcca gttcatcaag ggctcgtggc attatggcga gatgacttca tcaaaaaggc
    13321 tagagccaga gtttgagcta acagctgtag gaaaaggatt tgtaggaatt ttagatgaca
    13381 aagccccacg agaggcacat cagaaaattg cacaagaagc aatcgaaata agcaaagaaa
    13441 aaattctttc tgaaaaatat gacattgtta ttttagatga ggtcaattac gcagtgaatc
    13501 ttggcctaat tgaagtagaa cagatacttg atttgataaa aatcaagcca caaaacctga
    13561 ctctggtttt gacaggaaat tatgcaaaac aacaagtcat cgatgctgct gatttagtaa
    13621 ctgaaatgaa ggaaattaaa catccctttc agcgtggaat tcgggcaaaa aaaggaatag
    13681 acttctagcg gcaacattaa attatgtagt ccaagccaaa gcttgcaatg gctacagaat
    13741 cccaagagct tccagaaatt ggcgagattg taattgcgac tgttgcaaag cttaccgatc
    13801 acggcgcata tgtgacactt gatgaataca acagcctgca gggatttttg cacgtatctg
    13861 aaatagcacc aggctgggtt cgttccatta gcaagtttgt ccgagagggt gaaaaaaaag
    13921 tcctacttgt aaaaaaggtc aacccaaaca gatccgaaat cgacctttca ctcaaacagg
    13981 tatccaagga gcaacaaaag aaaaagctct tagaggcaaa aagaattgag aagggcaaaa
    14041 cgcttttgca ggcagtcaaa gaatcagcag ttttgacgga taaggatctt gaaaagctag
    14101 aggacacaat tttttcaaaa tatgattttg tttatgacat gttcttagat gtggtaacaa
    14161 agggcattac agccatagat gagctaaaac tgccaaaaaa aaccctttta gcaatagagg
    14221 aggtcagtgc aaagatcaaa ccaccgtcgg tggaaatacg tggcatttgt gagctcacat
    14281 gtaacaaatc agacggtgtt gatattatca aaaacgcaat tttggattca attggggagc
    14341 atatctccaa cattgaagtg acatacattg gtgcgccaaa gtacagaata agcgtaaagg
    14401 gccaagactt taagacagca gaaaggatgc taaagccggt tttggacaaa atccaaaaaa
    14461 taatcgaaaa aaacaacggt acctttaatt ttgcaagaga agagtcaaag aaaacccgag
    14521 agggataaaa ttgaagtttc agattcgcaa gtgccctgac tgcaaaaaat atactctaaa
    14581 ggacaattgt tctcagtgca attccaagac cataacggtt catcctgcca agttttctcc
    14641 tgatgacagg tatgccagat accgcataaa ggagaaattc aaagaagatt aaggagtgga
    14701 aattgttatg gtcttaattc tcgcactttc ccaatccatt ggttgatcat ttaggtttgt
    14761 ctgaagtcca gcaatttggt ctacaacatc ttggccttct attacttcgc caaagatcgt
    14821 gtattgtccg tctaaccaag atgcctcacc caccattatg aaaaactgtg agcccgcact
    14881 gtcgatgtct tgaccacgcg ccatggacac aacatacttt gtgtgcttga gatcacttat
    14941 ttcagctgga attgaatatc caggcccacc tgagccccat gttcctgggg gaccagatat
    15001 ggtgtttgga tctcctccct gaatcataaa gtcaggtatt attctgtgga agagagttcc
    15061 atcataaaag ttagagtcgg caagcttgac aaagttttca actgtctttg gcgcaatctc
    15121 tggctttaag tcaattacga tatcaccaaa agtagttgag attgttgcaa tttgatcttg
    15181 agtcgcctct gtagtttctg tagtttccgt cgttacagca ttttgttgtg ggttgtaatc
    15241 cttgttaacc tcgtaaatta gtactccgtt aattggtcct gagtcttttc gtaggaatcc
    15301 agaagacatg taagctagtt ttagtgggcc atcgccatcc tgtggaaatt tggtattatt
    15361 tgtgtagaca ggcgtaaagc caggaacaaa tccatttgac tgcttttgat ttgcaagatc
    15421 cacatatgca agggtgtcgt atggaatcat ttttccaagc atggtttcat cccaaaattc
    15481 agcagttggg cttatgccgt cctgatagag atacttttcg acaggaaatc ctcctattct
    15541 catgaaccat tgctttttgc tttcatcgcc accaccttga acaatgtaca aaggtggctc
    15601 ttcgttgttg acacgctgag cagcaatgta aactagcaca taatctgcgt ccaattcttg
    15661 gagtattttc caggcttcat ctggtgtact cagaaacatt tgtgcgattt tcttaatttt
    15721 ggaatctatc agagttgcat tatctgcaag agattttctt tctcccagtg tagttatcca
    15781 gtatccatag tcccaccagg atgcgacaac agcatcttct ggagtgtttt ctttcaacca
    15841 tgccattgca tcaagccaat ctgttgttga tatgttgaag ttacttccac cgttaagtat
    15901 ggttggggga acctgcgcac tgtttatcca gtttgcacca gctgggacag tcagcggcac
    15961 tattaacaga attgcaatta tagcagcgta ggaaattttg agtgtttttg ggtgtgattt
    16021 gataatcttt ttcttttctt gttggtcttg cctaaaaatc tcttttgtaa tcaatgagag
    16081 tccaactgat cccaatataa caagagagag tgaagaaaac aattctaatc tgacgaatgc
    16141 agagcttgtg taaactccta acaatccaat aattaaggca aatacgtgca tgtcacgttt
    16201 gccagagtcg ttgagaatat ttttgaagat aagccatgcg ccgattgcac caaatatcat
    16261 aaaaaccgaa ttaaacaaaa atgactgaaa tattgttgta gtagagtgtt cagcaacaga
    16321 gtcaaccaaa ggatcttccg tagtcaaaaa cgggtttatt gcattcagat acctaaaact
    16381 tggaaggcct agcacatcag ctgaaagtat accaattcct gccacaatta tggctattaa
    16441 gaacaatcca ccgtttcgtt ttttgtgctc ctccttgctt agttgttgaa taaacgtgca
    16501 ggctacaaga aaagccgtag gaccaattag cataaagcca gttgcatggg taaaaatcgc
    16561 aattcctggc ctctcaagtg gagcaagtga tattgccaag cctaaaataa atacaggaac
    16621 agcccatctc agaaagttac catcctttcg cacaaacggt aatgaaagta tgaatagacc
    16681 tagtggtagg acaaaaaatt ccgttccacc ccatgctgaa aaggcaaaac caagaaatat
    16741 tccaccacca attaatttta ggaaagcaac cttgcgtgag gatgatttca atccactcaa
    16801 gaagagataa actgccaaca gaccataaaa cagtcctagt ggctctgact tgaaccaacc
    16861 aattgtacct ctcacaatta tgggtagtga aactgcatat agtaacgagg caaacaggcc
    16921 tgcagttgta ccaccaatta ctctaactag ggcaaaaaca acaattgcag ttaacgaacc
    16981 aaaaactact gggaatatga ttgtaaaacc atatagatct gatcccccac cgaatgcacc
    17041 ataaagtatt gcggcagtta tatgcaacat agcttgggat gttgaagcca catctctgcc
    17101 atgtggatgc cagctcatct catcatgcca tgaaaaatat gcaggcagac cgttatctac
    17161 tataaactgt gttgccctgt aattaaaaaa gggatcaaat tcgtttagct gaaaaccata
    17221 ctctgctggc tgtgatctaa tcatggcaga gattgaaaat gacagtgaaa gaattccaat
    17281 tatcagcaag tgatgcagac tgaattcgaa attacctaca gaaaatagtt ttttattaga
    17341 aatcaatcga ttctttccca gaaaatgtgt ttaattactc ttttgtgaga atttgtacct
    17401 gacatgatgt cacgagtttt ttgaaggtct ttgcgtcctc ttcgcttccc acaactgtac
    17461 cataatgcat aggaatggca agttttggct ttattttttg agttgcgctt gcagcctcgc
    17521 tggcagtcat cacataagta ccacttactg gcacaaacaa gacgtctggc tgaatatcac
    17581 tcatttctga aatgttatca gagtcccccg tgtggtaaaa tgcagtatta tttattgtga
    17641 caataaatcc gactttgttg tcttcctttg gatggaatgg tttttttgtc tctggattta
    17701 ttttgtcaat gttgtaggca ggcaccgttt ttatttttat accattaatg gctttttcct
    17761 ggtttggaag catgccaatt atttccttat tttttgcttt aatttgccct agacattctt
    17821 ttgctgccac tatgattgta ttatcgccag ttattttttt gagatcatca ttactcatat
    17881 gatcaaagtg attatgagaa attaaaacaa tgtctgctga aatctttttg tcaagctggt
    17941 atggatcaaa acaaactgtc agattgctgt gtgttaacgt aaatgtatca tgcccatacc
    18001 atctgatttg aacaccattg tattcaatca tgtttctcac tatctacaaa acaagttaaa
    18061 ccttactgga ttcaagtatt attttgttgt ttgccacaga aataacagag cccccttctt
    18121 tttttattgc agcctttaat tccctgtcca ttgtcgcaac aattccacca tgttttttga
    18181 tatgtgatac aacaatatca tctgcaaagc ttccaccaag cttcattatt ttatatgatt
    18241 ttataaaatc caaagttgca agtgcgacgg tttttttgtc gtcaagctgt gatagtttgg
    18301 tcagctcagc aactacagtg tctggaacaa caaactggac tcgccctatc tcgacttcaa
    18361 gtgaggagag atttttgatc ctattagttg ccagatgaat aagaaaactt gtgtcgcata
    18421 ttatctcaac caattacacc tgctccaatc agtctccacc tttcctcaat ttttctgctt
    18481 atggcaacgt tgctattctc aaataggcat actggccttt tgaattgtac ctcgattttt
    18541 ccggacttgg ctttgcttac ctttgcaaga atgggggcag ttccaatgtt gagcctcagt
    18601 gattcaccca tctgaattgg gattactttg acgtcatgta ttgtcccaac ggcagaatca
    18661 aacagattta cctctagttg tgcagtaata gaattttctg gcagtgtttc cggcttgcca
    18721 ataacagagc caacaaaaga gtcgcttcgt gtcagcgaag gatcaagctt tgttccaatt
    18781 gcaacaaggc cgccaggctt gacattatca acaattccgg ctgcagttcc cagagaaacg
    18841 atttgtgtgc gtattggatc atagttgttt ttcttgctgc taagaattcc tggttttatt
    18901 tcgatctcat cgcctattga aaatgttcct tgggtgaggc ttccgccaat cactccacct
    18961 ttgatgtctt tgatttttgt gccaggcttg ttgacatcaa atgatcttag aacgtgcatt
    19021 actggttctt ttgattccat tctctttggg gttggaatcg actcctcaat ggcaccaatt
    19081 aatgcatcaa tgtttagtct tgattgtgct gagattggaa ttattggagc cttgaccgac
    19141 ttggtgcctt tgacaaattt aacaatatca gaataattat tcatggcatc ctcgtatgat
    19201 accaaatcaa ccttgttttg aacaattaca atttgctgga ttcctaggat ttgaagggcc
    19261 aaaaggtgtt cttttgtttg tggttgggga accttttggt ttgcggcaac aagcaataat
    19321 gcgccatcca ttagtgcagc accagaaagc atgtttgcca tcaggctttc gtgccctggg
    19381 ctatctacaa aactgacaac tctagacagt tcgctttcct tgccacagtt aggacatttt
    19441 ggtatggttg aatatccaag tgggggctca cagtttttgc acttgtaaaa agcagcatcc
    19501 gaatagccta ctcgaatcgt gattcctcgc tttagttcct ggctgtgaac gctagtccac
    19561 tgaccagtaa gtgcttgaat caaagtggtt ttgccatggt ctacgtgtcc tgcagttcca
    19621 atgtttacac atggttggta gccatatttt tttacatacc aatccggtaa agtgtcacgc
    19681 cagtgcatcg ataaatctca aaaagtctgc tatgttaagt tatgctttgt tttctgctgg
    19741 agcttcttct attttgagct cgccatcata tgaacagtta atctggtaga tttcttctga
    19801 aattgtattt cctcgaatta gtcgtcttcg tcgttgtcct ttttcgatat tttgcaatcc
    19861 cactcctttt gagagtaata caggctttcg tgcggaacca tagatatcag aacgcattgg
    19921 cattccagac ttgtcgcttc ctccggtgat tttgatcttg cctgcaagac caactactac
    19981 tgcgtcaagc tcgtttccta tgtgaaggcc gataagtgag tttgcttctc tgtcctttag
    20041 ttcctttgat agggactttc cttttttgtc agatattgtt agcttaaagt tagccaagta
    20101 ttgctcaaga aatttgagca actaattaac gttttgacaa actctttaac ctagatttgc
    20161 gtaatttatc gcaaatgaaa gaagagatta cttgtccgcg ctgcaacaaa aaaatggttg
    20221 acatgcaggc atgtcatatg atatgtgata attgtggggc tcaccttgac tgctcagaca
    20281 agggctcttt ttggtaaact agaatcccgg cctctttgga atgtaatcag atgccatgtt
    20341 tatggccttg tccttcatga tttcaaggta attttcatag tcggtctcaa agttgcagtg
    20401 agggcatttt acagacttca tatcatcgtc taggactgct actccctcac attctggaca
    20461 tttggcatcc atgcttgtat attctcttaa agctatttaa tgctagttca gaaaatttag
    20521 tttgtgcaga gttagtccag cccgacatga cgtcagattt acggtgaaat ttagtttttc
    20581 ttttgccaga aattgctagt tgtttacaat tgatttgata taactcaata gacatccttg
    20641 atatcttgga tattacacaa tgaaaacaga tgaaaagatt ggaaacgcaa catgttgaag
    20701 atatcatatc atggaataaa aaagagacaa atgaaagaaa agaatcattt ggtgtcaaac
    20761 tagaagtatt gaatggtatc gtcgacaagg taaatctgct gtcagatatt ttagatgaca
    20821 aagaacgcgt attaagacaa atgtctacac tgataggatt gattgtattt gagcaaccct
    20881 ttaacaatgg caataaagca actgctactt cttcggcaat tcagttcttg agaacagatg
    20941 ggtatgaatt ggatttagaa tcagagaaag ctcaggatga gttattgcag atgttggata
    21001 gtattatgta tctgtttgaa gaccagtcag aacaaggaat tattgcagta agaaaatttt
    21061 tagaaaaaca cttgaaataa tctacatttt ctctagtttt ttgaagagtc ttggattgtc
    21121 ttcttttact ttaatcatac catttaggaa agaggtttct ttattctcgg tttctatttt
    21181 tactaccata gttatgagtg gatgattcaa tcgtaaaaag gtttgtgatt attaattgcg
    21241 taatagtcga tcctgattac tagaaatagt aattatttta ctgatcagta agcggtgtta
    21301 gtccagcccg gcaggacgtc agcttcccaa gctgaaggtc gcgggttcaa atcccgcaca
    21361 ccgcacctac agttctttta tggcctgtag aatgttctct ttgtcttttg catttggtgc
    21421 aaactttaga tagttttcaa agtcttgctt tgcttccttg tttttcttta gctccagtaa
    21481 tgcaatgcca cggtttttgt aaattgggcc aaagcgagtc tggttgatat ctattgcttg
    21541 agaataatat gagattgctt tttcatactg ttttgtcttt agataatagt ttcccaaacc
    21601 tcggtatgcc aaaaagcatt ttttgttgtg cccaatgctc tgctcataaa agttgacaca
    21661 gtctggagag tttttctcat tgtatattcc gccaataaga cataatgcag tcgagttcgt
    21721 cccatcaaga cttatggcgt cgtttagcgc gttgattgcc tcatcagtct tgttttgaag
    21781 gttaagtttt tctgaaacaa agcaaagtcg attggatttt tcttttgatc tgttctcaac
    21841 tcttatggtg gaaatgatat cagtaggggc aacaagtatg agaagccgcc cgtcctcctc
    21901 ccaaagtctg tcaaactgtt tttctggtat gactcctatt tggtctggct gcggcatgta
    21961 gtgaatgatt gatttttcct tttcatcata tcctgagatg acagatgcgt gctggactat
    22021 ttcaaagagg ccgggcaaaa tcacgattgg gggaattccc atgtcaatta tttttttgag
    22081 ctcagaaagc gacgaatgaa gaatcaggct tccaaggccg tgccgctcag caagctccat
    22141 tccctcgatt agaatgctgc cacgaacgtt tggatacttt tttgcaattt cttttgcctc
    22201 tgcaactggc agattaacgt cccaatattt tgaaactgca ctgactgcca agggtaggca
    22261 gatgttttcc tctgttactg gaggaagtaa cagctcgtgt tcgctcataa aattgcattg
    22321 tgaatttgtt attaatagta tcagaacttg gtaaaccttt caattactga taggccgtct
    22381 tttgacattt cagggtgaaa ctgagttcca aatattgtca aattttgata ttgtattatt
    22441 tcgtagttac aagtatcaga ttttgcaagc tgaattaatg attttcctag acgcgatatc
    22501 tcgtagttat ggctttggta tgcctgtatc ataccgctac aaaggggatt ttcagctacg
    22561 actgtgactt ggtttgttcc tttctgtgtt gttgacattt tccgtatagt acctccaacg
    22621 gttagagcca aaatttcagc accataacat attccaagca atgatttttt ttctgagatt
    22681 gaatggttga ttatttttga attaactgcg ttcatttctt tgttgttttg tcttctgcca
    22741 gacaagatga atgattgaaa tttgctcagt tctgataata taactttgtc aaatggtaaa
    22801 attgcaaagt cgatgctctt tttttccaaa aggaattgga caatgttttt tgtaaaaact
    22861 gatccattgt caaccactaa tagcatcaca gttagaccaa gtatgtttct tttataatat
    22921 gatgacagga agatgctaca agatttatct tgttgttcac ttgcccactt cgattgacac
    22981 ataatggagc tcaggtatgc catccactat ggttatagtt ccaatattta ctgcctgatc
    23041 tacttcccac cagagcacac gttttgtcga tggtagtaca aagtcatcta tgaacttgtt
    23101 tagcatctcg tttgttgcat caagatcaga atgactaaac agtgagatct cgccttcttt
    23161 aaacgaaatt ggaatttgaa gcgatgttgg agttacaatc tcgatgtcat tttcctcaaa
    23221 tactgatttt atttgttgga ttctggcatt tttgtcaaac ccaacctcac ctgtaggtgt
    23281 tgagggaaca acaacgcctg ccagttcacg caagtatgcc tcaaaggcct gatgctgggt
    23341 gtttccaaga ccaacataat attccccatt aaatgcggcg cccactgctg caattgttcc
    23401 tagctgagct accactccac cgccagaccc tgcggtatat actggaataa agtaaacatc
    23461 gtgatcacca accctataca aaatattatc accaatacgc ggtgttctca atagcgtctt
    23521 taattgtgca aagtcactgt ccttgtctaa tgcctcacgc accgcagttg ggccaattag
    23581 ctttgtagta gagtttagcg gaacttcgta aaatcgcatc tgccctagtg ccggcatatc
    23641 gttttcaaca atcatgtagc ctgaaaggtt ctttccttgg gaacccctaa gctccagtga
    23701 caagagtccc aaaaatttgg tgctctcata tcctggcggc tttgcctcaa tgtaatacgc
    23761 ctcaagggtg gccggaattt cataaaattc gtttgcctgg atgaatgtct caatgtcagt
    23821 cacatgatag atgttaaaca tctctgtttt ccatgtgaaa agctcctctg gatatctgat
    23881 ctgctcgttt agccaagagg gtatcggctc gaatttatct ccatactggc tttccaatat
    23941 ttttgtaaaa tagctgtcgc cgtactcgag cagagttatg tcgccattat aagtatcaat
    24001 taatgcaaat cctacaagac gcagatatgg attgcccatg gcccatggaa cagaatgtgt
    24061 gtcaaatcca ataattaggg gaactagcca ataggtattt gttccatcag ttactggaag
    24121 gacgtccagt tgttggccaa acaaactgta tagaaaatac ggataaagta attccattct
    24181 gtcgtgtatg tccttgtatc gcagtgtatg aattggcgtt gctggatatg acaaaagaaa
    24241 gttaggctca aaaatccagc tcagcggcgg cgaaatttgg actcctccac ttcctgtgta
    24301 aaatgcattg tttagctctg cacttacgct gcccctagtt gttggatagg cagaccatgt
    24361 ctgctcaaag agtccgcttt ctccataata gattgccctc tgcgagaaga gattgctgct
    24421 atctattgtc tgtcccgtag ttgcatcaag tgtgagaaaa ccgtttggaa catgagtata
    24481 caccaagtgt tcgttgtacc agcggttctc taggctaaca gatgatggta agaccggctt
    24541 catagatacg gtccaataca gggtgctgtt gaatctcaga acgtcgttgt cctgaaactc
    24601 gatatatggt atcagaccaa tttctggtct taattttgcg gatgctgcct cccagtccca
    24661 tattcgtatc gaatccaaca cgtcactgtt ttgttgcata aagcttctga tgttgtttgg
    24721 ggaaatcgat gtgagtctaa cttcgtttgt agtggttttg atcttgtcta gttctcctag
    24781 gtatctgttg acttcgattt gttgtagagt gtatggacca agatattcca gtttacgagt
    24841 gtctgcaatg ctgttgttta ctgccataac agagccggct atgattccaa ttgcgattag
    24901 tgtaaagact cggatgtaga tgtctcgttt tattggatgc gttagtattt tggatcggat
    24961 cttgtcaagt actgaaaatg caatgagtgc aaagcctgca gcaagcgttc caccaattac
    25021 ataccgcgtg ttgtaatcaa tgtgttctgt gaaaaacatg ttgattccgg cccaaattat
    25081 gccgattcca attattgcct caattgtgga gatgtagttt aggaactttg gtttcccttg
    25141 tgaggagtcg tgtatgtatg aggtaatcac ttttacaatg tggtgcaggc cgacatagat
    25201 tgccagtctc attccaatga cgccaagcag gggcggtaca agtatggtaa gtgccggaat
    25261 gagtgtgatt acggtttggg ctgcgtctgt agaaaatggt gtgctaaatg gtaatgcgaa
    25321 aagatttggc aggttgttga tacccaaatc attgccctca agcatgtaga caaacgccac
    25381 accaaacatt atgttaacaa aaaatgcacc aaataggaag accttggtta tctgccagat
    25441 gacaaagtga ggaacgctta gcttgtagtc cctaaagctt ggaatgtttt ttgagacagg
    25501 atcgtgaatg gttctgttca aaaatgtgat tgccacatgg atagcgtacc aaagaataga
    25561 ggagcggttc tttatgtcca cattgactag ggcaattgcc gaaaggacga ctgccgagat
    25621 tagagaatag tagagcggtt ttgtgaattg ctcgccaaat tcggtcatat tcattgacag
    25681 tatgacgcct tggtttccga ctatagtcag aactataatc cctatgactg cgattatgcc
    25741 taggcgtatg tacttgccag catctggttg cggtgcattt gtttcagaag atgagttata
    25801 caaaatcaag gttgctcctt tgtggacaaa ttaatgtctt acccgttttt tgaagcaaga
    25861 ctaagctaag gtagcgcatc ctttgcagat catatatact gccgcatgtc ttggaatggt
    25921 gacttgttct tttttgtacg ggccaaattt tttgcctttt agtcgaaatt ctactggaca
    25981 gtctgctaac acagtaacat tgtcgtcacc tatgaaacat gcatcatttg atggattgaa
    26041 ttttgaaata tcctcacaca gaatttttac aagaccgctt ttgctgttta ttgtgccacc
    26101 taatctgaat attctgtgta catcgattgt tacacttggg tcgatctgca ctccaatttg
    26161 cctttggagc tcctctagct tccttttaaa tgtcatatag ccttctgaga tgatttgctt
    26221 tgaggccttt gccttgttcg attttgagcc aaagatctgc tttgatacac ggccaagcca
    26281 gcctttgtca tctggttcgg caaatgtgga ttttgcaaaa ttggatagtt ttgcgccaaa
    26341 tgtctctggc atggcccccc tgaacatcaa ataatctact agttctgctc tttcttttga
    26401 ttctagtttt tcatagctag aatttgggat atgtacatga aagccctcgt tgccagaaaa
    26461 ataggtttgg atattttcat tttttattcc cagatcggat tctaaaatgc tgatgagctt
    26521 tttaacctcg ttttttgcag ccaaaatgca gtctttgcac aaaacagata ctgtatcaaa
    26581 tttggcagaa ttgcattgtg gacattgttc ttggtttgca aaaacatttg cgcagtttga
    26641 gcatttcttg cagatatgat tggttctgca aggaaggttc agatcctttg cgtctatgtc
    26701 aaatataaga tcggcttttt gccagtcttt ttctgccatt ggaagatttg gaaaagaata
    26761 acgcgcattt gagcagtaaa catctgaagg aacctgcgtc atcagtagca ggtgtagctc
    26821 cttatccgat tttaaagaga tgtgcctgtt catgccagaa ttgaatttct gatatccgaa
    26881 ctctcgctcc tctgggtttg caggagtgtg aatcagatca aaatgatcaa aataatattt
    26941 tttgaaggat tcttctacta gctttatttc cttttcaagc attggttatt tttttccttc
    27001 caaattgtaa tgggttgatg atgttatcac attctggtat ggcatagcaa agattttcac
    27061 ttttcagctt atcacatgat ggacatgagt attttgttcc gctaccaaag tttccagcta
    27121 ggtgttttag ctggtacagt gttacctttt cgttatagtc aggcgcgttt ttgaacaacg
    27181 gcgcaatatc ctcaattgtt tgacctttgt tcaaaagata cgttgcaagc atgaatcttc
    27241 cagagtgcgg caggttttct cccttgctga gaacctcgat tgcgtgcttg atgcatggtg
    27301 ggtactccgt tgtctctaca aattgcacgg tgaatttttt tgccaagtca actagatcac
    27361 ttactggctt tttgaatgaa tctggtacta ggggaatgct tgcagactgg atttttgagc
    27421 tgataaaatt gtcaagctcc tttcgaatca gccttactgt ctcgtgcgcc gaaagaaaga
    27481 cgtttccatt gtccactagt cggtttacca gcttccattc tttctcatga aaatgagttg
    27541 cgtgttttag atagtctggt actggaatta caaaataatc gtcgtgtttt tgaatctcca
    27601 ttgcaaaaag atcccttatt attttgattg ccagctgttc ttttgttgta ttattgatat
    27661 cggcaaggtc tttttcaaga aatttctctg cacgccttgc ctctgccaaa gaaaatcgct
    27721 taatcagcgt gttcatgccg ctttgtttta acaagattac tgcaataaga aaggaaaaga
    27781 cttcaatgtc cagattcttt atgctaaaat cagaattgaa gatcttgcca tttgctgcag
    27841 tctcgattct agaaagcgcc agcccaatta ttggccggag atcttcgtct ttgtcaaatt
    27901 gctctagtgt gaatcctttc tctctgagat agtttcctgc ctctgtcaaa aacggatatt
    27961 ttgctatctc ttctgttccc agctttaaca catatgttat gatttggttt gacattaaaa
    28021 atttgagttt tgttctatat tgtttaaatt atctagatct aaccgatctg tatgcgagtg
    28081 ggccttcatg tctctatttc aggatcgctt gctgctgcag ttgataacgc agtagagcgt
    28141 aaatgtagcg cttttcagat ttttacgcgt agcccccgca tgtgggctgc aaaggacata
    28201 tccgaaaagg atgcgtccga ctttagagaa aggcttgcct caagcaaaat agacaggctt
    28261 gcaaccgtag cacatatgcc gtatctgcca aatcttgcat ccccaaatgc tgctactcac
    28321 gcaaaatctg tagatgtact aatcaaagaa gtcaagcggt gtgcacagct tggcatccca
    28381 taccttgtag cacatctagg cagccacatg ggagaaggtg atgaaaaggg gataaaacag
    28441 ctagtcagcg cgttttccaa agcagcaagc gtcaaaaatg atgtagtgat tttgcttgag
    28501 aatactgcag ggcagaaaaa ttcggttggt tccgagtttg atcagtgggc taagatcttt
    28561 tttaagctaa agccaaagga aaggtttggt gtctgttttg atacgtgtca tgcgtttgcg
    28621 tatggatatg atttgaggac agaaaaagat gttactaata cattcaaaaa atttgaagat
    28681 gcaataggat ttgagcatct aaagatcttg cacctaaatg actcaaaagg tgagatcggc
    28741 tcaaatctgg acagacacga gcatattggg cttggcaaaa ttggcgagaa aggcatgggt
    28801 gtaatagtaa ggcttgccaa caaaaaagac atccccataa ttttggaaac accgattgat
    28861 aacactcggg acgactttgg caatataaaa aaggtaaaag aacttgcata gtttggaatt
    28921 tattctcaat gtttggtgaa gactcgtatt gaactacgac agcataatgc agcttgcact
    28981 tgagcgtgga ttttactttc ctagctgcga gatttactct gatgcacagg ccggtttttg
    29041 ggaatatggt ccctctggcg taagcatgaa gaccaagttt atcgagttgt ggcgccgcga
    29101 gcttgtgcgc cgtgacggaa tgcttgaaat tgacggctcg caaataatgt cacagtctgt
    29161 ttttgaagcc tctggccatt tagcaagctt tgcagatcca atagttcgat gcaaacaatg
    29221 cggtttgaac tttagggcag acagattaat ctttgagcaa accaaaattg agatcccaga
    29281 aagtgccgat cttggagact ttgataagac aataatggaa aaaaatatca aatgcccaaa
    29341 atgcaaaggc gagtttgaca agacgcgcaa gttcaacatg atgttcaaag tggaaattgg
    29401 tcctgaagca gagccagcct atctgagacc tgagacctgc cagtccattt ttgttgattt
    29461 cccaagacta ttcaaaacaa tgcgcggcaa gctcccactt ggaattgctc agattggaaa
    29521 aagctttcga aacgagatct caccaaggca gagccttctg aggttgcgag aattttacca
    29581 agcggaaatt gaggtttttt gtaatccaaa caagctaaat gacttggaaa gattttccga
    29641 agtacagaac acaataattc gactttggat taatgatgag tcaaaggcaa tgagctgcaa
    29701 gcaagcagtc gactctggga ttctgccaaa caagtttgtg gcatactatc ttggtattct
    29761 gacagagttt tacgaaaaaa ccggaataga tatgacaaag agcagattcc gactactggg
    29821 agaaaaggaa aaggcattct atgccaccgt tgcctttgat tttgaggttc aaaccgcaat
    29881 aggatggctt gagcttgttg cgtgtaacta tagatcagac tatgacctct caagccacgc
    29941 aaacaaaagc aaggaaaaat tcgaagtgct agatgatgag caaaaagtcc tgccgcatgt
    30001 atttgagata tcgatgggaa tagacaggag tttgtacacg attttagaac atggtctaaa
    30061 agaggacaag caaaacgaca gaattgttct ttctatcaag ccatatttgg cgccaatcca
    30121 cataggagtc ttgtctttgg tcaaaaaaga cggtcttaag gaaaaaaccg atgagattta
    30181 cctcaaaata aagcgaaaat acgatgcgtt tttggatcat tctggggcaa ttggccgaag
    30241 gtatcgaaga cttgatgagg taggagcacc ctttgccatc actgttgatc accaaacact
    30301 acaagatgat acagtaacac taaggcatcg cgattcgatg caacaagaca gaattaaagt
    30361 ttcagatatc gactctgtgt tatctaatgc aatctcattc ccatagacaa taatctgatt
    30421 ttgaatctat tttgtctcat taccacgata aattttttca aaaaaaacta gtctgaggtt
    30481 gttgttatga gttttatgta gttagctctg tactttacct tgaggcgcac atcctctaat
    30541 gcgtgtgctt ctgggatcac tccatcgtct gattctaacg taacaatcca gtttccaaca
    30601 attggatcaa ttgaggttat agagaatcgc tcaagtggac tgtcttgccc tgaatatctg
    30661 attctgtata tttgtccatc tagatctacg tttaccgaat ttcctctaaa tcctgcagtc
    30721 gaaccactta caaggcatgg atcagattta ccaataatac atgttccatc cggtgctact
    30781 attctgtagt gcaaggacgg gttagtatca gatgagtaga gcagaccggc tcttgccacc
    30841 acatcgccat tttccataac ggagtttatg gtaacaggtt ctgaaattga gaatattctt
    30901 gtcgtttgga atgttctaag aattagtgat gtagtttcga ttgtcttatc gtataggtta
    30961 agttgtgcgc taacctgata ctttgattcg gctcgtacgt taggtatttt ccactcaaag
    31021 ttaactaccg actcgccctt gttgaataat tgttctggtg atgtgtacac tatggtgcca
    31081 ttcacaagta gtgacgctgt tccaaacaat ggtttttcat catcattatt cacgtatgca
    31141 gttggcctgt acattgtgct ttctgccttg cttggtggcg agtcaagcat gattcttgca
    31201 tcaaactcgt atggcggctt tactccaagg atatatcttt ctgagctttg tgcctgcccg
    31261 tcgttattta agacgtaaat ccaatagact gttgcaggtg tctgcaggaa caatgacggc
    31321 agatctactg ataccaagta tgcgtttgtg atgttttgta gtggcgcgat cttcattact
    31381 actgctgcat aatcaccata gtttccaccg gctgtactta ccctaagctc cgcccgtcgt
    31441 attgtagatg ggctgtcaac tattgcactg aatttgagtt gggcgcctgg ttcgacgaat
    31501 tggtttatct ccgttgcctt gattggtttg ttaccatcta cttggaattt aatatcaaag
    31561 atttgtggtc gttttacacc aacagtagtc tcagaagttt ctgtcctttc aatatcttcc
    31621 atcgaattga caacaattac gtttctacac tcatctacgt ttaccaagta tctggcaatt
    31681 accacatctc ttccagctat ttgctcagca gtcacaataa agaagtccag ttttggatct
    31741 agcggcgctt cgtagacata cctgcttgtc gagaatgctt tattggcctc tgcgtatggt
    31801 tggtcttgaa ctagtgttgc tgagtagatc ttctgagttg gcgttcttat ctttacattt
    31861 ggtggtggtg tttctaagcc ttgaatgccg actatgattc tgatcatatt ttgttcacac
    31921 aaatcatacg atatttcata gatcactatt ggttgaacta gctttcctcc aaatcccgta
    31981 gctcctgctg cggctccccc accacctcca ccaccgccac caccagatgc agctggagta
    32041 cttggtgaag gagcgctggg ggttgcagaa gaggtatagc ttgcaaaact agtaaagtgt
    32101 cttgtccaaa cgtacagatc gcttccactt gtgatcttgc aatttccgcc tgcgccaata
    32161 aatgcggtat ttgttggttc atcgctaaca gatgg
//
LOCUS       DASWKH010000071         7339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_177433, whole genome shotgun sequence.
ACCESSION   DASWKH010000071 DASWKH010000000
VERSION     DASWKH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177433"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGA92_05230"
     CDS             <1..592
                     /locus_tag="VGA92_05230"
                     /inference="COORDINATES: protein motif:HMM:NF020962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX9845849.1"
                     /translation="GKWAEMPFVIVSEIVRSYYIGFWSALNYWGMTEQVPSVTFVVTT
                     NRKRNLEYGPLKFKFITFSKNKFFGIVEEEIGGEKFRISSREKTIVDCLIYPKYCGGI
                     DEVVKGIWEAQNELDFTKIIDYSKKIGTKVVIKRLLYILDILNIKTNVNKEVLKDDKG
                     FMWFDPSGPRKAIEYSKEYGLIINRTQRDLMSWKGY"
     gene            593..1384
                     /locus_tag="VGA92_05235"
     CDS             593..1384
                     /locus_tag="VGA92_05235"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9845850.1"
                     /translation="MMNKDDIMRMAGEKKLNPSAISKDYALGWLLFGVSKSSIGSNLI
                     FKGGTALSKVHFPEGWRLSEDLDFTLVDDINVNVLEKVLREDVPKIIKEAIGMDVRLK
                     DRPHINEGYYQSRFQYVGPLGKDTVKIEITREATIGKPETKKMPKVFDYQAFDVKVYS
                     LEETLAEKMRSIIQRTKIRDYYDVWRLLKTKRFDREKVRALFLEKCKSKDVAFTSVDQ
                     FFPDGIVKTLEPYLETGLTRLSREPLLPLQDIIGELRESLETFLK"
     gene            complement(1498..3021)
                     /gene="guaA"
                     /locus_tag="VGA92_05240"
     CDS             complement(1498..3021)
                     /gene="guaA"
                     /locus_tag="VGA92_05240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX9845851.1"
                     /translation="MDRIVVLDFGSQYSHLICRRIREFSVYAELVPYDISLEKLQQLN
                     PKGVIFSGGPSSVYNTDSPKPDARIFQLKLPLLGICYGHQIIVDNFGGKVKRANKEYG
                     HSVLTVDNGSDLLSGIGGSVRAWMSHGDEAEKIPNDFEVIGHTERSHAAAIANKQQSI
                     YGIQFHPEVVHTENGVQILKNFVLNICGAKQDWTMESFVEKTVSDLAKIEGNILCGVS
                     GGIDSTVAALLIHKAIGDRLKCVFVDNGLLRQNEEREIEKMFSENFAVNFTAINAKKQ
                     FLDKLKGVSDPEEKRKIVGEEFVRIFTEFAKKNGSFKWLAQGTLYPDVIESGVSKGPA
                     AVIKTHHNVGGLPDWLDLDVLEPLRDLYKDEVRKVAAILGVPRRLLMRHPFPGPGLAV
                     RVIGEVTEKKLSIAKLASKIVEEELESAGWYEKVWQAYAAVGDDKAVGVVGDERKYGN
                     IVMIRVVESIDAMTADWSRLPHDILAKISNRITNEIEDVTWVSYVISSKPPATIEPQ"
     gene            complement(3026..3568)
                     /locus_tag="VGA92_05245"
     CDS             complement(3026..3568)
                     /locus_tag="VGA92_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845852.1"
                     /translation="MQFSEDQIKDALEIKERLSSEIQKHKEQIEMLEKNLTLINSILK
                     QQSFTKASTLKDTKEPPKQDQVIPLRKNTDGSLIANAYVTPNEVSIVLENGVELKEDT
                     HPFKSFFLERIIGDMKKKDLADFEKGEIDENAVINCLVNKNGALLREIIIKNYRQKER
                     INEIISTVTWSFSKMIDNSK"
     gene            complement(3604..4341)
                     /locus_tag="VGA92_05250"
     CDS             complement(3604..4341)
                     /locus_tag="VGA92_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HEX9845853.1"
                     /translation="MTVTGWNLQYLKMLDEFGYSKKMDLKAAAMLDSILDRWFSLVKL
                     KKLIFGKSVFVIGAGPSLQSAIPVLKKYKNIIKICADTALAPLLQSGITPQIVVTDLD
                     GDLTSLRRIAKKCIVIVHAHGDNMDRLEFAKNFKNCIGTTQTKEIGKICNFGGFTDGD
                     RCVFLAEYFGAAKMFLFGMDFGPKIGRYSKTKKRDRKTKLKKLRFAKMFLEWMAPKTH
                     SKLFTLSKPIRGFKKITYKQIEHNVSI"
     gene            4377..5246
                     /gene="folP"
                     /locus_tag="VGA92_05255"
     CDS             4377..5246
                     /gene="folP"
                     /locus_tag="VGA92_05255"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9845854.1"
                     /translation="MSRLGPVRVGGSNPVRIMGILNTSPESFYKKSIKKTSQIAEAVK
                     QMEEDGADFVDVGGMSTAPYLSTMVSEKQETKRITEAIKQIQKATNLPISIDTCRAAV
                     AKAALEYGVDIINDISGLQYDKQMPNVIEKHQPSLVLCAYSKKTLKGDLLVQAKLAIQ
                     SSLKIAKSANIKTSNIVIDPAIGFFRKTGKHPFYTKINQDWFKRDLDVLQNLSFFKKL
                     NYPILISVSNKSFIGKLLDKKNPADRLYGSIAAEVIATLNGADIIRTHNVKETKDAVS
                     VAQVFSKKIKKSL"
     gene            5277..6707
                     /gene="guaB"
                     /locus_tag="VGA92_05260"
     CDS             5277..6707
                     /gene="guaB"
                     /locus_tag="VGA92_05260"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9845855.1"
                     /translation="MEIREGLTFDDVLLVPKYSNITTRAQTDLRTKLSKNISLNIPLI
                     SANMDTVTESAMATAMAREGGIGIIHRFLSVQEEVEEVLKVKRSASVIIEKPYYIAPE
                     QTVKEAIAYMHEKGVSGLLVIKDSKLAGILTGRDVMFESASNKLVKDVMTKDVITAKP
                     GISLGEAKEILMQHRIEKLPLVDENNTVRGLITSKDITHNEDYPNASKDKKGRPLVGA
                     AVGVKGDFMERAEALLEADADVIVVDIAHGHSENAINTIRNIKKAFPSCELIAGNVAT
                     AKGAEDLIKAGVDAVKVGVGSGSICITRVITGSGVPQLTAVYDCAQVGKKHGIPIISD
                     GGTRTSGDVTKALAAGASTVMIGSLFGGTDESPGSYVMKNGKRYKIYRGMASFYAALG
                     RKSKETGIVAINEDLNDYVAEGVEAMVPYKGTVTDILKQLTGGVRSGLSYCGAHTIPQ
                     MQENAEFIKMSRAGFAESQPHDVDLM"
     gene            6738..7202
                     /locus_tag="VGA92_05265"
     CDS             6738..7202
                     /locus_tag="VGA92_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845856.1"
                     /translation="MLNKMTGIGIGVGAVIVAIGLYALVSSIGLQTVTVDEKIDIGDS
                     TTYQFLAPKSSHQNLKVTGDSFHVKLETPADGIQKDEDFKNEITFDWYVLAEGTNRIS
                     IQNTGQSELNVIGTFEKNTDPILFTYHIMVLTAGVVIIGFSAGFSIRKPKGF"
BASE COUNT         2275 a         1457 c         1520 g         2087 t
ORIGIN      
        1 cggcaaatgg gctgagatgc cttttgtcat agtatcagaa atagtgcgct cctattacat
       61 agggttctgg tctgctctaa actactgggg aatgactgag caggtcccaa gtgtgacctt
      121 tgtcgtaaca acaaacagaa agaggaatct ggagtacgga ccgctcaagt tcaaattcat
      181 caccttttcc aaaaacaaat tttttggcat agtcgaagag gagatcgggg gagagaaatt
      241 cagaatatcg tccagagaaa agacgattgt agactgcttg atctatccaa aatattgtgg
      301 aggtattgat gaagtagtta aaggaatttg ggaagcacag aatgaacttg actttacaaa
      361 gataattgat tactcaaaaa agataggaac aaaagttgtc atcaagagac ttttgtacat
      421 acttgacatt cttaacataa agacaaatgt aaacaaagaa gtgttgaagg acgataaagg
      481 attcatgtgg tttgatccaa gcgggcctag aaaggcaata gaatattcca aggaatatgg
      541 tttaatcata aacaggactc agagagacct gatgagttgg aagggatact aaatgatgaa
      601 caaagacgac atcatgagga tggctggcga gaaaaagctc aacccatcag caatcagcaa
      661 ggattatgca cttgggtggc tgttgtttgg cgtatcaaaa agctccatag ggagtaatct
      721 catattcaag ggtggtactg ccctttccaa agtgcatttt cctgaaggtt ggagactttc
      781 cgaggacctt gatttcacct tagttgatga tatcaatgta aatgtcttag aaaaagtgtt
      841 gagagaagat gttccaaaaa taatcaagga ggcaataggt atggacgtca gattaaagga
      901 cagacctcat atcaacgaag ggtattatca gagtagattc cagtatgtcg gcccactggg
      961 caaagacaca gtgaagatag agataacgag agaagcgaca attggaaaac ccgagaccaa
     1021 aaagatgcca aaagtctttg actatcaggc gtttgatgtc aaagtatact cgctagaaga
     1081 gacattggca gagaaaatgc gttccatcat acagcgcacc aagatcagag attactacga
     1141 tgtttggaga ctgctaaaga cgaaaagatt cgacagagaa aaagtgaggg cattattctt
     1201 ggaaaaatgc aagagtaaag atgtcgcctt tacaagcgtt gatcaattct tcccagatgg
     1261 catagtgaaa acgttggaac cttatttgga aaccggcctt acaaggctca gtagagaacc
     1321 actgttgcct cttcaagaca tcataggcga gctcagagaa tcattagaaa cgtttctcaa
     1381 gtgaacgcag caaattgaat taacttctag cggaatctca cacatagtaa ttctgagttc
     1441 taatcaaccc ttttagttat tcaaattttt caaatctagc taaacagcaa tacttcttta
     1501 ttgtggttct attgtggcag gcggcttact tgatatcaca taggaaaccc acgtcacatc
     1561 ttcaatctca ttggtaattc tgttgctgat ttttgctaaa atgtcgtggg ggagtctact
     1621 ccagtctgca gtcatggcat caattgattc gactactcga atcattacaa tatttccata
     1681 ttttcgctca tctcctacaa cacctactgc tttgtcgtct cctacagccg cataggcctg
     1741 ccagaccttt tcgtaccaac cagcagattc aagctcctct tcgactatct tgcttgcaag
     1801 cttggcaatt gaaagttttt tttctgtcac ttcaccgatt actctgactg caaggccagg
     1861 tcctggaaac gggtgtctca taagaaggcg tcttggaact ccaagaattg cagcaacctt
     1921 tcgaacttcg tctttgtaca ggtctctgag tggttcaagt acatccaagt caagccaatc
     1981 aggaaggccg ccaacgttgt gatgcgtttt gatgacggcg gcagggcctt tcgaaacccc
     2041 actctcaatc acatcggggt aaagggttcc ttgcgcaagc cacttgaagg aaccattctt
     2101 tttggcaaat tctgtaaata tacgaacaaa ctcctcacca acaatttttc ttttctcttc
     2161 aggatctgag acgcccttta gcttatccaa aaactgtttt tttgcgttaa ttgctgtaaa
     2221 attaacagca aagttttcag agaacatttt ttctatttca cgttcttcgt tttgccgcaa
     2281 aagaccgttg tctacaaaaa cgcacttgag cctatctcct attgccttgt gaattaaaag
     2341 ggctgcaact gtggagtcaa ttccgccact cacaccgcac aagatatttc cttcaatctt
     2401 tgcaaggtct gatactgttt tttccacaaa gctttccatt gtccagtctt gctttgctcc
     2461 gcaaatgttg agaacaaagt ttttgagaat ctgaactcca ttttctgtgt gaactacttc
     2521 gggatgaaac tggattccat aaattgactg ctgcttgttt gcaattgcag ctgcgtgaga
     2581 gcgttctgtg tggcctatga cttcaaaatc atttggaatc ttttctgcct catcaccgtg
     2641 gctcatccag gctctcaccg aaccaccaat tccagaaaga agatcggatc cattatctac
     2701 tgtgagaaca gagtggccat attctttgtt tgctcttttt actttaccgc caaagttgtc
     2761 aactataatc tggtgcccgt agcaaattcc aagcagggga agctttagct gaaaaattct
     2821 tgcgtctggt tttggtgagt cagtattgta aacacttgat gggcccccag aaaagattac
     2881 cccctttgga tttagctgct gtagtttttc aagagaaatg tcatatggaa caagctctgc
     2941 atatactgaa aactcgcgta ttcgcctgca aattaggtgg ctgtactgag aaccaaaatc
     3001 caatacgaca attctgtcca ttttatcact tggagttgtc aatcattttt gaaaatgacc
     3061 atgtcactgt gcttattatc tcgtttattc tttctttctg gcgataattt ttaattatta
     3121 tttcacgcaa aagcgccccg tttttgttta caaggcagtt aattacggca ttttcatcaa
     3181 tttctccttt ttcaaaatcc gctaggtcct ttttcttcat atctccaatt attcgctcaa
     3241 ggaaaaatga cttgaaagga tgagtgtcct cttttagctc tacgccgttt tctaatacga
     3301 ttgagacctc atttggagta acatatgcat ttgcaatcag agaaccgtct gtgttttttc
     3361 gcaaaggaat tacttggtct tgttttggcg gttcttttgt atcttttagt gtggatgctt
     3421 ttgtgaagct ttgctgtttt agaatggaat taattaaggt gagatttttt tcaagcattt
     3481 cgatttgttc tttgtgtttt tggatttctg agctgagtcg ttcttttatc tccagggcat
     3541 ctttaatctg atcctctgag aactgcataa ttgttctgaa gtattgacag tattaaatcc
     3601 attctagata gaaacattat gttctatctg cttgtatgtt atctttttga aacccctgat
     3661 tggctttgat agagtaaaaa gcttagagtg tgttttgggt gccatccatt ccaaaaacat
     3721 cttggcaaat ctcaattttt tcagcttggt ttttctgtct cgtttttttg tctttgaata
     3781 tctgccaatt tttggaccaa agtccattcc aaacaaaaac atcttggctg ctccaaaata
     3841 ctctgccaaa aatacacatc ggtccccatc tgtaaagcca ccaaaattgc aaattttgcc
     3901 aatctctttt gtttgtgtag tgccaataca gtttttgaaa ttttttgcaa actcaagcct
     3961 gtccatgttg tccccatgtg catgaacaat tactatgcat ttttttgcaa ttctgcgtag
     4021 agatgtcaga tctccgtcta ggtcggttac aactatttgc ggtgtaatgc cactttggag
     4081 tagtggtgca agcgctgtat ctgcacaaat tttgataatg tttttgtatt ttttgagaac
     4141 tggtatggct gattgcaacg atggtccagc cccaatcaca aatacggact tgccaaaaat
     4201 cagctttttt agcttgacta aagaaaacca tctatctagt atcgaatcaa gcatggctgc
     4261 agcttttagg tccatttttt tgctatagcc aaactcgtct agcatcttca gatattgtaa
     4321 attccatcca gtaactgtca attactctaa aaatggcatg ctttatcata aatgatgtga
     4381 gtaggctagg cccagtaaga gttggcggtt caaatccagt gagaataatg ggcatactaa
     4441 atacaagccc ggagtcattt tacaaaaaat caatcaaaaa aacaagtcag attgcagaag
     4501 ctgttaaaca aatggaagaa gacggtgcag actttgttga cgttggaggc atgtcgactg
     4561 caccatatct ttcaacgatg gtttcagaaa aacaggaaac caaaagaatt acagaggcaa
     4621 tcaaacaaat ccaaaaggca acgaacctcc caatctcaat tgatacgtgc agggcagctg
     4681 tagcaaaagc agccttagag tacggcgtcg atattatcaa cgatatttct gggctgcaat
     4741 atgacaaaca aatgccaaat gttatagaaa aacaccaacc gtctttagtt ttgtgcgcct
     4801 acagcaaaaa aacactcaaa ggagatctgc ttgtacaagc aaaacttgct atacaatcaa
     4861 gccttaaaat cgcaaaatct gcaaatatca aaacaagcaa catcgtaatt gatcctgcga
     4921 ttggattttt tagaaagaca ggcaagcacc cattttatac aaaaataaat caggattggt
     4981 tcaagcggga tcttgacgtt ttacaaaacc tttctttttt taaaaaacta aactatccta
     5041 ttctgatatc tgtttcaaac aagtcattca ttggcaagct tcttgataaa aaaaatccag
     5101 ctgatcgact ctatggttca attgctgccg aagtaattgc aacgctaaat ggtgcagata
     5161 ttattcgcac acataacgta aaagaaacaa aggacgcagt atctgtagca caagtgtttt
     5221 caaaaaaaat caagaaaagc ttataatgca tattaaatct cctttagagg gtttaattgg
     5281 aaataagaga aggattgacg tttgatgatg ttcttcttgt gcccaaatac tctaacatca
     5341 caacacgggc ccaaaccgat cttagaacaa aactctcaaa gaacatctcg ctaaacatcc
     5401 cgttaatttc tgcaaacatg gataccgtta cagagtctgc tatggcaact gccatggcgc
     5461 gcgaaggcgg aattggaatc attcacaggt ttttgagtgt acaagaagaa gtagaagagg
     5521 ttctcaaagt aaagcgttca gcaagcgtaa taattgaaaa accgtactat attgctccag
     5581 aacaaacagt caaagaggca attgcgtaca tgcatgaaaa gggagtctca gggctgctcg
     5641 taatcaagga ctcaaaactt gcaggaatcc taactgggcg tgatgtcatg tttgaatccg
     5701 catcaaataa gctagtcaaa gatgtcatga caaaggacgt cattaccgca aaacctggaa
     5761 tctcacttgg cgaggcaaaa gaaattctga tgcagcacag aattgaaaag cttcccctag
     5821 tagatgaaaa caatacggta agggggctca taacaagcaa agacattaca cataatgaag
     5881 actatccaaa tgcatcaaag gacaaaaaag gaagaccact tgttggcgca gcagttgggg
     5941 taaagggaga ctttatggaa agagccgagg cactacttga ggccgatgcc gacgtaatag
     6001 tagttgatat agcacacggc cacagcgaaa acgcaatcaa cacaatccga aatatcaaaa
     6061 aagcatttcc aagctgcgag ctaattgcag gaaatgtcgc aacagcaaag ggagcagaag
     6121 acctaatcaa ggcaggagtt gatgcagtaa aggtaggtgt tggttctggc tctatttgca
     6181 taacacgagt catcacgggt tctggcgtgc ctcagcttac tgcagtgtat gattgtgcgc
     6241 aggttggaaa aaagcacgga attccaataa tctctgatgg ggggacaagg acttcgggcg
     6301 atgtgacaaa agcactggca gctggcgcat ccacagtaat gattggaagc ctctttggcg
     6361 gaacagatga aagtccaggc tcgtatgtta tgaaaaatgg aaaaaggtac aaaatctata
     6421 ggggaatggc ctcattttac gcagcgcttg gaagaaaatc aaaggaaacc ggtattgttg
     6481 caatcaatga agatctaaat gactatgtgg cagaaggagt agaggcaatg gttccgtaca
     6541 aaggaaccgt aacagacatc ctcaagcagc ttactggcgg ggttcgctca ggcctcagct
     6601 attgtggtgc acacaccata ccacaaatgc aagaaaatgc agaatttatc aaaatgtctc
     6661 gtgcaggctt tgctgaaagc cagcctcacg atgtagattt gatgtaagtt tttattttct
     6721 gattttaact gatttttatg cttaacaaga tgactggcat cggaatcggt gtcggtgcgg
     6781 taattgtagc aatcggacta tatgcattgg tatcgtcaat aggacttcag acagtaacag
     6841 ttgatgagaa aatcgatata ggtgattcta ccacttatca gtttttggca ccaaaaagct
     6901 cgcaccaaaa cctcaaggtg actggagaca gctttcatgt caagctagaa acaccagctg
     6961 atggaataca aaaggacgaa gatttcaaaa atgaaattac ctttgactgg tacgtattag
     7021 cagaaggcac aaacagaata tcaatccaaa acacgggcca atcggagctt aatgtaattg
     7081 gaacctttga gaaaaacacg gatccaatct tgtttacgta tcatatcatg gttctaactg
     7141 caggcgttgt gataattgga tttagcgcag gatttagcat ccgcaaacca aagggcttct
     7201 aaactagtct tgcttttttg taggagccga gcactttgag aaacaacgaa tgctgaccaa
     7261 tctttgataa aagttccttg atttttggtt cgtcttgatt tccgtcaaaa tcaacgtaaa
     7321 agttgtattc ccacggcgt
//
LOCUS       DASWKH010000072         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_565030, whole genome shotgun sequence.
ACCESSION   DASWKH010000072 DASWKH010000000
VERSION     DASWKH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565030"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="gcvPA"
                     /locus_tag="VGA92_05270"
     CDS             <1..963
                     /gene="gcvPA"
                     /locus_tag="VGA92_05270"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX9845857.1"
                     /translation="PEASQGMLQAMYEFQSFICLLTGMDVSNASLYDGASALAEAVLL
                     SSSYTKKRQVFVDDGLNPNYYEVLKTYCNGSDLEFTNIIDENTCCVISQNPDFYGNIK
                     DLRPLVEKAHHNKALFITCVNEPTSLALIKPPGEYGSDIVVGEGQSFGIPLSFGGPYL
                     GFLAVKNFLLKKIPGRIVGMTEDKNGKKGFVLTLQAREQYIRRERATSNITTNQALLA
                     LAATIYLATVGRKGLVDVAKNSYYKAHLLYEKLQKAGFKALNEKYFYNEFCVKTPKTA
                     SKIFSQLLEQKIVGGLVVDDEKMIVCCTEKNSMADINAYLEALK"
     gene            960..>1529
                     /locus_tag="VGA92_05275"
     CDS             960..>1529
                     /locus_tag="VGA92_05275"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX9845858.1"
                     /translation="MKLIFEYDQQPTEHVEQIQTQLDLPIELVRDNLPLPNVNETDIV
                     RHYTKLSKKNFGIDNNLYPLGSCTMKYNPKVSEELARLKGFASIHPRSPAEYSQGSLE
                     VIWELQEALKEITGMDDVSLQPAAGSQCELLGIMIAKRHFENKNEKRTKVIIPDSSHG
                     TNPASVAMCKLETITVCSDSRGNMDFNEVK"
BASE COUNT          526 a          285 c          311 g          407 t
ORIGIN      
        1 ccagaagcaa gccagggaat gcttcaggca atgtacgagt ttcagtcatt tatctgcctt
       61 ttaacaggaa tggatgtttc aaatgcatcg ctatacgacg gagcctctgc cttggctgag
      121 gctgttttgc tttcttcttc gtataccaag aagaggcagg tctttgtcga tgatggcctg
      181 aatcccaatt actacgaagt tctaaagaca tactgcaatg gctctgattt ggaatttaca
      241 aacataatag atgaaaatac ttgttgtgta atttcgcaga atcctgactt ttatggaaac
      301 ataaaagatc taagaccact agttgaaaaa gcacatcaca acaaggcact gtttatcact
      361 tgtgtaaatg agccaacatc tttggcactc ataaaacccc ctggagaata tggttctgac
      421 attgttgtag gagaaggtca atcatttggg attccactaa gttttggtgg accatatctt
      481 ggatttcttg ccgtcaaaaa ttttttgcta aaaaaaattc ctggcagaat agttggaatg
      541 acagaagaca aaaatggtaa gaaaggattt gtcttaactc ttcaagctcg agagcaatat
      601 atcagaaggg aaagagcaac aagcaacata acaacgaatc aagcgctgct tgcacttgct
      661 gcaaccattt acttggcaac agttggaaga aaagggcttg ttgatgtggc aaaaaattcc
      721 tactataagg cacatttgct ttacgaaaaa ctgcaaaaag ctggctttaa ggctttgaat
      781 gaaaaatact tttacaacga gttttgcgta aaaactccaa aaactgcaag caagattttc
      841 tctcaacttc ttgaacaaaa gattgttggc ggactagtag ttgatgatga aaaaatgatt
      901 gtatgctgca cagaaaaaaa ctctatggca gacattaacg cttatctgga ggcactaaaa
      961 tgaagctcat ttttgaatat gatcagcaac caacagaaca tgtagaacag atacaaacac
     1021 agcttgatct accgattgaa ctagtacgcg ataatcttcc ccttccaaac gtaaatgaaa
     1081 cagacattgt acgacattat accaagttat caaagaaaaa ttttggaata gacaacaacc
     1141 tgtatccttt gggttcctgc acaatgaagt acaacccaaa ggtaagcgag gagttggcaa
     1201 ggctcaaagg atttgcatcg attcatccac gttcacctgc cgaatattca caaggctcct
     1261 tagaggtaat ctgggaattg caggaggcat taaaggaaat tacaggcatg gatgatgtat
     1321 cacttcaacc tgctgcagga tctcagtgtg agcttttagg gattatgata gcaaaaagac
     1381 attttgaaaa taaaaatgaa aaaagaacca aggttatcat tccagattcg tctcatggta
     1441 cgaacccggc aagcgttgca atgtgcaaat tagaaacaat cacagtttgc tcagattcta
     1501 ggggaaacat ggactttaac gaagtaaaa
//
LOCUS       DASWKH010000073         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2779805, whole genome shotgun sequence.
ACCESSION   DASWKH010000073 DASWKH010000000
VERSION     DASWKH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2779805"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGA92_05280"
     CDS             <1..282
                     /locus_tag="VGA92_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845859.1"
                     /translation="GGMSAESEAIYKAASHAIIICRQDKKGELAKWRDFLEKLGVELV
                     AIIISKLDGNENIQTNDLIEAELVSLDRNPRLSPATRQLASLLKSKLGI"
     gene            complement(265..912)
                     /locus_tag="VGA92_05285"
     CDS             complement(265..912)
                     /locus_tag="VGA92_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845860.1"
                     /translation="MSSAETIKVVDGQERKIAAGGGSKLFYLPKETGDYLQIGDTIVL
                     SVIIEGNELKIVGTKQLFYFGVKQIRDLIKEFGFETKYDKNVENIVVLDAAKGNLSLN
                     YTQNLHKKIEQAHITVAVELNNVSYGSYKKISRLGAQLGKFNVIIRPQGDLDTINLLK
                     DSGHYKLDGKKAFEVLKKAGKKIGVSVVLRFNNKQNEIEDVRAGIVELSKLNSKL"
     gene            complement(948..1205)
                     /locus_tag="VGA92_05290"
     CDS             complement(948..1205)
                     /locus_tag="VGA92_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845861.1"
                     /translation="MTKQRRTVTLSKELDDIIAILASGKQMNYSEFLETRLRTLPEVA
                     KTVERINSMPEEPALVKTVSRQGMHQKSRAQRKLQKPQQKK"
     gene            complement(1300..1806)
                     /locus_tag="VGA92_05295"
     CDS             complement(1300..1806)
                     /locus_tag="VGA92_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845862.1"
                     /translation="MREAVEITNDLGQIIVVGAVAVMIHTQRGRQSADIDIAIAIKLS
                     DDEMVLKKYIPIRGKRDSWDSPRGVKIDIFRRDVSGIPVETIAKTAKNVVVDKNGTKI
                     KTASLEVLIATKYRAFNRRKSLGDESDLRIIAKRKFKEIDWNLLGQITTEMEFADIKR
                     AITAYYNS"
     gene            complement(1799..2509)
                     /locus_tag="VGA92_05300"
     CDS             complement(1799..2509)
                     /locus_tag="VGA92_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845863.1"
                     /translation="MRNQSFQEPKRWKDLSKAVLFASGLSGKLVYSIKNDAIPLAGTA
                     PTAYDRLHILESLGLAKLTRNTFQINTSVVYQPLYIIKKLQPSLDALQQAKRFGKYYT
                     QYDINFVNKHLPGKFLKTLDYAAWDLTKFQTPQDLFIYVDDIEMSSKFLLDNGFNEGE
                     NGRVILLPKTGSFENEIERVYLDSIANGGRSILDAVAIELLYRNELTVQGRFSVEQII
                     KVLEDLRSYRNIHEPISA"
     gene            complement(2725..>3060)
                     /locus_tag="VGA92_05305"
     CDS             complement(2725..>3060)
                     /locus_tag="VGA92_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845864.1"
                     /translation="LNVTEDFGFVRINPDEKFGEITKVMINGKELDADCTTGCTSTIY
                     SNQDLNVEVWNVWGGRAFSHLEKSEEIVLDDEINWDLVFVVIVVAVAGLLLWKFAGQA
                     LEHFGFRRV"
BASE COUNT          807 a          642 c          577 g         1034 t
ORIGIN      
        1 ggaggcatgt ctgcagaatc cgaggcaatc tacaaggcag cttctcacgc aataattatt
       61 tgtaggcaag acaaaaaagg cgagcttgcc aagtggaggg attttttaga aaaactgggt
      121 gtggagctag tagcaataat aatctccaag cttgatggca atgagaatat acaaacaaac
      181 gatctgatag aagcagaact agttagtctg gatagaaacc ctcgtcttag cccagctaca
      241 agacagcttg cgtccttgtt aaaatcaaag cttggaattt agctttgaga gctccacaat
      301 tccagctctt acatcttcta tttcgttctg cttgttgttg aagcggagta caactgatac
      361 gccgattttt tttccggcct tttttagcac ctcaaatgcc ttttttccat ccagcttgta
      421 gtgaccagag tcttttagca ggttgattgt gtcgaggtct ccttggggcc tgatgatgac
      481 attgaatttt cccagctgtg cgccaagcct gcttatcttc ttgtatgacc cataggagac
      541 attgttaagc tctactgcaa ctgtgatgtg ggcctgctct attttcttgt gcaggttttg
      601 tgtataattt aacgaaaggt tgcctttggc tgcatcaaga accacaatgt tttctacgtt
      661 tttgtcgtac tttgtttcaa agccaaactc cttgattaaa tccctgatct gctttacgcc
      721 aaagtaaaac aattgtttgg tgccgactat ttttagctca ttgccttcta tgattacact
      781 gagtacgatg gtatctccta tctgcagata gtcgccagtc tcttttggaa gatagaataa
      841 cttgctgcct ccgcctgctg ctatttttct ttcctggccg tccacaactt ttatcgtttc
      901 tgcactactc atttccacta ttatatagtc gtgtaattat ataaagttca ttttttctgc
      961 tgcggttttt gtaatttgcg ctgtgccctt gatttttggt gcatgccttg tcttgaaact
     1021 gttttaacca acgccggttc ttctggcatg ctgttgattc gctcaactgt cttggctact
     1081 tctggcaatg tccttagtcg ggtttctaaa aactctgaat agttcatctg tttgccagat
     1141 gccaaaattg caatgatgtc atctagctcc ttgctgagtg tgactgtcct tcgctgtttt
     1201 gtcatactat tatcatatca tagtaatcat ataaacatat tggcttgaaa aaaaatcaca
     1261 cccttctaaa cccaaaatac tccggggcct gcctttcact tacgagttat aatatgcagt
     1321 tattgctcgt ttgatgtcgg caaattccat ctccgtagta atttggccta aaaggttcca
     1381 gtctatttcc ttaaacttcc ttttggcaat tatgcgcaaa tctgattcat ccccaagtga
     1441 ttttctgcga ttaaacgccc tgtattttgt tgctatgaga acttcaagtg aggcagtttt
     1501 tatcttggtc ccgtttttgt ctacgactac gttttttgca gttttagcta tcgtttcaac
     1561 aggaatccca ctgacatcgc gtctaaatat gtcgattttt acaccacgtg gactgtccca
     1621 agaatcgcgc ttgcctctaa ttgggatgta tttttttaga accatctcgt catctgataa
     1681 tttgattgct attgcaatgt caatgtccgc tgactgtctt cctctttgtg tgtgtatcat
     1741 tactgctacg gcgccaacaa ctatgatctg gccaagatca tttgttattt ctactgcttc
     1801 acgcactaat gggttcatga atatttcgat atgatcgtaa atcttctaga actttgataa
     1861 tttgttctac ggagaatctt ccttgcacag tcagctcgtt tctgtacaat aattcaattg
     1921 cgaccgcatc aagtatactt ctgccgccat ttgctatgct atccagataa actctttcta
     1981 tttcgttttc aaaagaacca gttttcggaa gaagtatcac gcggccattc tcaccttcat
     2041 taaatccatt atcaagtaga aatttagagg acatctctat gtcgtcaacg tatatgaaca
     2101 ggtcttgggg tgtttgaaat tttgttagat cccatgctgc atagtccaaa gttttgagga
     2161 attttccagg caggtgtttg ttaacaaaat taatgtcata ctgcgtatag tactttccaa
     2221 atctttttgc ttgttgtaat gcatctaatg agggttggag ctttttgatt atgtataatg
     2281 gttgataaac tacactggtg ttaatttgaa atgtatttct tgtgagcttg gcaagaccga
     2341 gagactctag aatgtgtaat ctatcgtacg cagttggagc agtacctgcc aaaggaatgg
     2401 catcattttt gatgctgtac acgagcttgc cagaaagacc actagcaaac agtacagctt
     2461 tggacagatc cttccatctt tttggttctt ggaatgattg atttctcata aatgctataa
     2521 tatatgttaa acatataaag tttttaagat tacttaatat tattatatag ttgacacaga
     2581 atttatctat cagtagtata taagagtttg ttaggagaaa attcagaaaa acacacatta
     2641 ttaaaaaatg ttctaaagca cttaaagggg aaattctgct gaattagaaa ttcgtctctt
     2701 agatgaatgg tcaacatgat cttctcaaac tcttctaaac ccaaaatgct ccagggcctg
     2761 gcctgcaaat ttccacaaaa gcaggcctgc aacagctact acaatcacaa caaagaccag
     2821 atcccagtta atttcgtcat ccaaaacaat ctcttctgat ttttcaagat gggaaaaggc
     2881 gcggccgccc cacacattcc agacctcgac attcagatcc tggttggagt aaatagtact
     2941 agtgcagccg gttgtacagt ctgcatcaag ttcttttccg ttaatcatca ccttggttat
     3001 ctcgccgaat ttctcatcgg gattaatgcg gacaaaccca aaatcctctg tgacgtttag
//
LOCUS       DASWKH010000074         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2789814, whole genome shotgun sequence.
ACCESSION   DASWKH010000074 DASWKH010000000
VERSION     DASWKH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789814"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VGA92_05310"
     CDS             complement(<1..823)
                     /locus_tag="VGA92_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845865.1"
                     /translation="MISTNGIFKLFSVLVILMLFSSVSLGTVFAIDSPKKQLASGVAP
                     EDVICKQGLILAIIFNNVPVCLKPSTASELEKRWNTTLVFPEAAQPKADAPKNEPSTK
                     STTGATIQGQPGKTQALVEIIPASGGSTVNFYITDDDLNTSPNGVDIISTEGLLEFTI
                     NGIAIEGPETMIETGPNTGKFYVGLELPDTVNGQALTQNDVIVVRYLDQSDAAGESRV
                     SVTSVPLSQTYAQVQASGGGQSRIGHEFSVRIYEPDANLDSREVDRIPLSRIEFRA"
     gene            complement(929..1594)
                     /gene="fsa"
                     /locus_tag="VGA92_05315"
     CDS             complement(929..1594)
                     /gene="fsa"
                     /locus_tag="VGA92_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX9845866.1"
                     /translation="MKIFLDTANISSIKTYNDMGLLDGITTNPSLLAKEGGDPQKTME
                     EIVRIVKGDVSLEVVSTEYKGMIEEAHRLKKYGPNVVVKCPMTADGLKACKTLSSEGI
                     PVNVTLVFSPNQAVLAAKSGAKYVSPFIGRLDDIGEDGMALIAEIKQIFNNYNFKTQV
                     LVASVRHPMHVVEAAKIGADVVTLPPDVLGKMLKHPLTDIGLKNFLADWEKLKQENPK
                     VQT"
     gene            complement(1591..2553)
                     /locus_tag="VGA92_05320"
     CDS             complement(1591..2553)
                     /locus_tag="VGA92_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX9845867.1"
                     /translation="MTDMRTTYGKTLIDIGQENPNVVVLGADTSDSLKTADFGKKFPE
                     RFFNVGIAEANLVSVAAGLAISGKTPFASTYAIFLPGRCVDQIRNAIAYPSPSDKKGL
                     NVKLVVSHGGITVGADGGSHQQIEDIAIMRAIPHMSVLIPADTVAVGKLTWIISQKYG
                     PFYMRMARSATPLVHSESQEFEIGKGITLREGADCTITACGITVKMALDAADSLKQEG
                     ISCRVIDSFCIKPIDAALLEKSARETGCIVTCEEHNVMAGFGSAVSEVISETYPVPIK
                     RIGVLDKFGESARDNEIPLLLEKHGITSINIAKAVKDVIGKKRQ"
     gene            complement(2562..4229)
                     /locus_tag="VGA92_05325"
     CDS             complement(2562..4229)
                     /locus_tag="VGA92_05325"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9845868.1"
                     /translation="MGLNYYQIKKNVRKARKLVIRATSIAGSGHPGGSFSMAEIMGCL
                     FFKYLRYDPKNPNWEDRDKLILSKGHASPGLFSNMAVAGYFEPSELDTLRAFGSRLQG
                     HPDLKCPGVEFCGGSLGIGLSFSIGSALAARLDKKPTRIFTVLGDGETDEGQVWEAAM
                     AGAKYKVDNLTAFLDRNFIQQDSYTEKVMPLDEVLSGDDISEMWRDASRWKVGDKWRA
                     FGWNVIEIDGHRIEQIDSATKKAIQTKGVPTIIVSRTIKGKGVEHMEDNPKWHGQAPK
                     KEFVPIIDMEIESQSMIAPSIIAGDMSNLANEVKRCVNGRADYIHLDVMDGMFVPNKT
                     FDHTKIKELRPITVIPFDAHLMINEPVKHVRDYVDAGSDIITVHAEVCDASSFGEIHD
                     ILKASQVGVGLAINPDTELPEWSKQFIPKLDQVIVMSVIPGKSGQKYIESSHEKTQRL
                     AKTLSENNFDGYIEADGGVTLDNIGACFADGARAFVGGSAIIGQADVRQIIREFRHKV
                     AYARRKTLIQKAFELGGKELVAKWIELHTIGEKRNQLNVIAKELGYT"
     gene            4349..4729
                     /locus_tag="VGA92_05330"
     CDS             4349..4729
                     /locus_tag="VGA92_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845869.1"
                     /translation="MDLQDIVPVVIYDNRCYLCIQFARVVSFLAKGRLNLVGHYTNLG
                     EKLRNQILDSSALEMFWFVDGKTAYGGRAALCPLFRELTNGSGKKLQKTDGMEACDVE
                     CKTAKAVFVRSASLLTHSKTIPIK"
     gene            complement(4732..5004)
                     /locus_tag="VGA92_05335"
     CDS             complement(4732..5004)
                     /locus_tag="VGA92_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HEX9845870.1"
                     /translation="MSEDKQKPPTLFQEILDKFSALITAAFGLVAALAWNAAIQAFFK
                     EFFGEQETFMPMLIYAIIVTILAVIITFVVARAITKAKEKSSNLGL"
     gene            5134..>5358
                     /locus_tag="VGA92_05340"
     CDS             5134..>5358
                     /locus_tag="VGA92_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9845871.1"
                     /translation="MAQDTQIICPEIDVCREVSPSLIKHVLVPFDESEISYHAFEFAL
                     DLAKKYRAKLSIITIIHSNILASSFLQISSH"
BASE COUNT         1356 a         1242 c         1098 g         1662 t
ORIGIN      
        1 ctgccctaaa ctcgattctg ctcaaaggaa ttctgtcgac ttcgcgtgaa tccaagtttg
       61 catcaggctc gtaaattctc acagaaaact cgtggccaat cctactttgc cccccaccag
      121 atgcttgtac ttgggcgtag gtttgtgaga gtggcacaga ggtaacactg actctgcttt
      181 ctcctgcagc atcggactgg tccagatacc ttaccacgat aacgtcattt tgtgtgaggg
      241 cctggccgtt tactgtgtct ggaagttcta gccccacata gaatttgccg gtgttgggcc
      301 cagtctctat catagtttca ggtccctcaa ttgctatgcc gtttatggta aactctagca
      361 atccttcagt ggatatgatg tctactccgt tggggctggt attgagatca tcgtctgtta
      421 tgtaaaaatt cactgtagag ccgcctgacg caggaataat ttcaactagt gcttgtgtct
      481 ttccaggctg tccttgaatt gttgcccccg tcgtgctctt tgtgcttggt tcatttttgg
      541 gcgcatcagc ttttggttgt gcagcttctg gaaagactag tgtcgtgttc catctttttt
      601 ccaattctga tgcagttgac ggctttaggc aaactggaac attattaaaa ataattgcca
      661 agatcagtcc ttgcttgcaa atcacatcct ctggtgcaac gccgcttgcc agctgttttt
      721 ttggggaatc gattgcaaaa actgtaccca atgatacgga gctaaaaagc atcaaaatta
      781 ctagtacgct aaataatttg aaaatcccgt ttgtactgat caattgaaag ctgttcttgt
      841 ttttatttta aaaacgcttg gtaacaaagt taccccaatt ggaaaaaaac gattgaattc
      901 aaccctattt agttttctta gatttagttt aggtctggac tttggggttt tcctgcttta
      961 gcttttccca gtcggccaaa aagttcttta ggccaatatc tgtcagtgga tgctttagca
     1021 tcttgcccaa aacatccgga ggcagtgtga cgacatcagc cccaatcttt gcagcttcta
     1081 ctacgtgcat tgggtgtcgc acactagcta ctagaacctg agtcttgaaa ttgtaattat
     1141 tgaaaatctg ctttatttct gcaattagag ccattccgtc ctcgccaata tcgtccagtc
     1201 ttccaataaa cgggctgaca tattttgctc cggattttgc agctagtact gcctggtttg
     1261 gggaaaacac caaggttacg tttactggta taccttctga gctcaaagtc ttgcaggctt
     1321 tgagtccatc agcagtcatc ggacacttta ctacaacgtt tgggccgtat ttttttagtc
     1381 tgtgggcttc ctcaatcatt cctttgtact ctgtactgac gacttctaat gagacgtcgc
     1441 cttttacaat tcgtacgatt tcctccattg ttttttgcgg gtcgccacct tcttttgcca
     1501 aaagcgaagg attcgtagta ataccatcca gtagacccat gtcgttgtac gttttgattg
     1561 atgaaatgtt ggcagtatct aaaaagatct tcattgtctt ttcttaccta ttacgtcctt
     1621 tacggctttt gctatattaa ttgatgttat gccatgtttt tccagtaata gtggaatttc
     1681 gttgtcccgc gcagactcgc cgaatttgtc aagtacaccg attctcttaa tgggcacagg
     1741 atacgtttcc gatatcacct cagatactgc agagccaaag cctgccatga cgttatgctc
     1801 ctcacatgtt actatacatc ctgtttctct agctgatttt tccaatagtg cagcatcgat
     1861 tggctttatg caaaagctgt caatcactct gcaagaaatt ccctcctgtt ttagggagtc
     1921 tgctgcatca agtgccattt tgacggtgat gccgcaggca gttatggtac agtctgcacc
     1981 ctcccttaat gtgattcctt ttccgatctc aaattcttgt gattcagaat gtaccagagg
     2041 ggtcgcagac cttgccattc tcatatagaa cggaccatac ttttgtgaaa tgatccaggt
     2101 aagcttgcct actgctaccg tatcggctgg aatcagaacc gacatgtgtg gtattgccct
     2161 cataattgca atgtcttcta tttgctggtg agatcccccg tctgccccta ctgttattcc
     2221 gccgtgtgat actacaagtt tgacgtttag cccctttttg tcactaggtg acggatacgc
     2281 aattgcgttt cttatttgat caacgcaccg gcccggcaga aatattgcat acgtgcttgc
     2341 aaaaggagtc ttgccagaaa tggctagtcc tgcagctact gaaaccagat ttgcttctgc
     2401 aattccaaca ttaaagaacc gttctgggaa tttttttcca aaatcagctg tcttaaggga
     2461 gtctgatgtg tctgcaccca agacaacaac gtttggattt tcctgaccaa tgtcgattag
     2521 cgtcttgcca tatgtggtac gcatatcagt catcggtacg ctcaagtgta gccaagctcc
     2581 tttgcaatca catttagctg gtttcttttt tctccaattg tgtgaagctc aatccatttt
     2641 gcaactagtt cctttccgcc aagctcaaat gccttttgga tgagtgtttt gcgtcttgcg
     2701 tatgccactt tgtgcctaaa ctccctgatt atttggcgca catcagcctg cccaattatt
     2761 gcgctccccc caacgaatgc tcgtgcgcca tctgcaaaac acgcaccgat attatccagt
     2821 gtgacaccgc cgtctgcttc tatgtatcca tcaaagttat tttcagacaa agtcttggca
     2881 agcctttgtg ttttttcatg agatgactcg atgtattttt ggccagactt tccagggatt
     2941 accgacatta caatgacttg atcaagcttt ggtatgaact gctttgacca ttcaggaagc
     3001 tccgtgtcgg gatttattgc aaggccaaca cccacctgac tagcttttag aatgtcgtgg
     3061 atttcgccaa agcttgatgc atcacatacc tcggcgtgga ccgttatgat atcagagccg
     3121 gcatccacat aatcccgcac atgttttact ggctcgttaa tcatcaaatg agcatcaaat
     3181 ggaattacag tgattggtct tagctctttt atttttgtgt gatcaaatgt tttgtttgga
     3241 acaaacattc cgtccatcac atccagatgg atatagtctg cgcgtccgtt gacacatctt
     3301 tttacctcgt ttgccaaatt gctcatatca cctgcaatga ttgatggcgc aatcatggac
     3361 tgagactcaa tttccatatc aataattggg acaaattctt tttttggggc ctggccgtgc
     3421 cactttggat tgtcctccat gtgctcaact ccctttccct taatggtcct agacacaatg
     3481 attgtaggaa caccttttgt ctggattgcc ttttttgttg cagagtcaat ctgctctatt
     3541 ctgtgtccgt caatttctat tacattccat ccaaatgctc tccacttgtc tccgaccttc
     3601 catctgcttg catcccgcca catctctgat atgtcgtctc ctgatagcac ctcgtctagc
     3661 ggcatgactt tttctgtgta ggaatcctgc tggatgaaat ttctatccaa aaatgcggta
     3721 aggttgtcga ctttgtattt tgcgccagcc attgcagcct cccagacttg gccttcgtcc
     3781 gtttcaccat ctcctaaaac ggtaaagatt ctagttggtt ttttatcgag tctggcagca
     3841 agagcagatc caatggaaaa tgacaggccg attccaagtg atcccccaca aaactccaca
     3901 ccaggacatt tcaaatccgg gtgcccctgc agcctgcttc caaaggccct aagtgtgtca
     3961 agctctgatg gctcaaagta tcctgcaact gccatgtttg agaacagtcc tggcgaggca
     4021 tgaccttttg acagtattag cttgtccctg tcttcccagt ttgggttttt tggatcatac
     4081 cgaagatact tgaagaaaag acaccccatg atttctgcca tagaaaaaga gccgccagga
     4141 tgcccagagc ctgcaatcga tgtggcacga atcactagtt ttcgggcctt gcgaacattt
     4201 tttttaattt gatagtaatt aagacccata tctaatcaaa ttgcaaaatt ggttggtgca
     4261 actgccagtt catgatttga gacacccgct gaattaaata aaaagctata tcctcaaaag
     4321 tttaatctca aggactgcta tgtagatcgt ggatctgcaa gacatagttc cagttgtcat
     4381 atatgacaat agatgctatc tgtgcatcca gtttgccagg gtagtgagct ttttggcaaa
     4441 aggaagactg aacctagttg gccattacac aaatcttgga gaaaagctcc gaaaccagat
     4501 tctggattca tctgcacttg agatgttttg gtttgttgat ggaaaaacag catatggggg
     4561 aagggccgct ttgtgtccgt tgttcagaga gctgactaac ggaagtggaa aaaagctgca
     4621 gaaaacagac ggtatggaag cctgtgatgt ggaatgcaag actgcaaagg cagtctttgt
     4681 gaggtctgcc agtcttttga cacacagcaa aacaataccg ataaaataat tctaaaggcc
     4741 tagatttgag gatttttctt ttgcttttgt tatggctcgc gcgacaacaa atgtgataat
     4801 tacagctagt attgtcacta ttatggcata aattaacatc ggcatgaacg tttcttgctc
     4861 gccaaaaaac tccttaaaaa acgcctgaat tgccgcgttc caagctagtg ctgcgactag
     4921 accaaaggcg gcagtgatta gtgcgctaaa cttgtcaagt atttcttgga acagagttgg
     4981 tggtttttgc ttgtcttcag acatttcatt ggcttttcta aattttcatt aaagcaaatc
     5041 tagtaaaata atgtcaatta atcaatcaaa ctatcagaat gtggcagaat cgaacacact
     5101 atatcatata agaaaaaata ccgcagacaa accatggcac aagacacaca gatcatatgc
     5161 ccagaaatag atgtatgcag agaagtctcg ccgtcactaa tcaagcatgt cttggtgcca
     5221 tttgacgagt cagagatctc gtatcacgca ttcgagtttg cgctggactt ggcaaaaaaa
     5281 taccgcgcaa aactttccat catcactata atacacagca acattttggc aagctcgttt
     5341 ctccaaataa gcagccac
//
LOCUS       DASWKH010000075         5779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3177007, whole genome shotgun sequence.
ACCESSION   DASWKH010000075 DASWKH010000000
VERSION     DASWKH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3177007"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VGA92_05345"
     CDS             complement(<1..829)
                     /locus_tag="VGA92_05345"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9845872.1"
                     /translation="MNCRVEKSSLSGTITCPPNKSYTHRAIFLASLANGKSLIKNVLR
                     SRDTNATIEICKSFGAEIQGNENNLEVIGIDKFGTTDLIVDASNSGTSIRIAAAVAAL
                     REGKTVLTGDESLKKRPMKPLLDALESLGAKCSSNDGKPPLTISGRMRGGEVSIPGNV
                     SSQFISALLVAAPLTEKGVALNISSDLVSKPYLDATIITMEKFGVKVKVLDQYKKYKI
                     EPQQYKHATITIPSDFSSVALLLSAAVLVGRDLTLKASVGNLAQGDEAIIDILGKLGV
                     "
     gene            complement(819..1673)
                     /locus_tag="VGA92_05350"
     CDS             complement(819..1673)
                     /locus_tag="VGA92_05350"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9845873.1"
                     /translation="MTEVKATIHGAISIVNAIATGKGATLGISSTVEALVSTTEGSGI
                     RIEVDSQNISSRLINKVVEEAVPKKDLEKNKIEISLKSEIPTGYGLKSSSAISSAVSL
                     ACHKLFRSHFTDRQVLLAGVDASIATKVSLTGAYDDACACYFGGTLVTNNYERKIIRM
                     EKTPTNLAVVIFVPKSRKRGNIRQLRTIKDVFERAWSFAKDGNYWDAMILNGIAASSV
                     LNSDPKIISRLVESGALGASISGNGPAIAAIAKKDRIPHIRKVFSESEGKILTSEINN
                     KKAEVHEL"
     gene            complement(1673..2494)
                     /gene="aroE"
                     /locus_tag="VGA92_05355"
     CDS             complement(1673..2494)
                     /gene="aroE"
                     /locus_tag="VGA92_05355"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9845874.1"
                     /translation="MTKMFAVIGDPIDHSLSPNIHNAAYKALGLDCTYIAYRIPHGEL
                     KAGVESLKQIKITGFNVTIPHKIEMMKFLDEPSQECKIIGAVNTVTNNDGRLIGYNTD
                     MDGFLDPIKKRNLPVNGSSVLLIGAGGAARAIIAGFAKENAHKITIANRTKEKGQELA
                     KFAAGLGLESDAVSLDEAGQRAGSYKFIVNATSVGLKNEPSPISTKTINSENIVYDII
                     YMPMNTDLIVQSKKNSARVIYGYEMLLGQAALAFEIWHGIKAPYDAMKKVLVGGF"
     gene            complement(2497..3165)
                     /gene="aroD"
                     /locus_tag="VGA92_05360"
     CDS             complement(2497..3165)
                     /gene="aroD"
                     /locus_tag="VGA92_05360"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEX9845875.1"
                     /translation="MAYKTCVSITETTPRRLKETLYDALKKSEYAEIRFDFLRPEAVP
                     EALQLVKKHLRRCVCTLRPKAEGGRFRGNEAERASILKLIAEYNPYLIDIEYSTLGKN
                     KELLNYVRKTGTPILVSWHDFRKTPPLSRLAEKLQQMKKISKHVKIVTTASSVVDTTT
                     VLSLYRKQNSNLVAFAMGDFGRISRILCLYLGSPYTYVSLGRPVAPGQFSLDEVKSLF
                     ALQK"
     gene            complement(3176..4228)
                     /locus_tag="VGA92_05365"
     CDS             complement(3176..4228)
                     /locus_tag="VGA92_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HEX9845876.1"
                     /translation="MEKSKELVILPKVPKSQLGKFLAGLEGEGVKTVYLDPKLLGKSK
                     IASIYTSPNAKYVVLEKDAPRPKGKKVGRRFKVLSNSDIEKIFVEAKKGLDFVIIEVK
                     DWKIIPLENIIAKLHKMHTEIFALARTPEEVRKMFSILEIGVDGVIFEASSISEAREA
                     MVYLGTSNFELREAKIIDIKEVGDGERVCVDTASMLHKGEGMLIGSRSNFLFLVHNES
                     VGSSFTSPRPFRVNAGAVHCYTLSPDGTTKYLSELETGAEVLVIDSHGRARRATVGRS
                     KIERRPMLMIKAQTGDEVGGIIAQDAETIRFVRPGGHLVSVTHLKKGDTVLVHAKTAT
                     GRHFGMEVSDEYILEK"
     gene            complement(4215..5003)
                     /locus_tag="VGA92_05370"
     CDS             complement(4215..5003)
                     /locus_tag="VGA92_05370"
                     /EC_number="2.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HEX9845877.1"
                     /translation="MVTGLQIRMERILRKGKMLCIPMDHGISNGPIDGLESPAEIIQQ
                     CELRGLTSVIINKGILKSLPRPSRVGVLVHFSASTTLSTAPNRKMLTGSVEEALRLGA
                     DGVSLHINIGGKEEPEMLEQLGKIAEGCHQWNMPLLAMMYPRGENIKNPHDPEIVGHV
                     ARIGAELGADIVKTLYTGDVDSFAKIVKSTPVPIVIAGGPKAKTDSDILQMTEDAMKA
                     GAKGVTYGRNIFAHKNPAQMVEALGAIIFRKESAKEAAKKIGKK"
     gene            5129..>5779
                     /locus_tag="VGA92_05375"
     CDS             5129..>5779
                     /locus_tag="VGA92_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9845878.1"
                     /translation="MSQITEQIHSSDVGDILENSLLGNRPSYGDTVRLLKSNDVNLMG
                     LVAGHITKKRFGKKASFVNNIILNYTNVCITDCKFCAFYRPPGDDESYTLSLDQIEAR
                     VKTAWEMFGIRQVLIQGGHNPNLGIEYYEDAFRMIRKKYPLVGVHGLSASEIDMIAKI
                     EKTSTKEVLLRLKEAGLQSVPGAGAEILVDSVKEIISPKKISSDEWIRIMGEAHAIG"
BASE COUNT         1350 a         1359 c         1261 g         1809 t
ORIGIN      
        1 tgaccccaag ctttccaagt atgtctatta ttgcctcatc accctgtgct aaatttccaa
       61 ctgaagcctt gagtgtgaga tcgcggccaa ctagtactgc ggcagaaagt agcagtgcaa
      121 cacttgaaaa gtctgagggg attgttatcg tagcgtgttt gtattgctgt ggctcgattt
      181 tgtatttttt gtattggtct agtactttga ctttaacacc aaatttttcc attgtaatga
      241 ttgtggcatc aaggtatggt tttgatacga gatctgagct tatgttaagt gccactcctt
      301 tttctgttag cggtgcggca actaaaagtg cagagatgaa ctggcttgaa acatttccag
      361 ggattgatac ctctccacct ctcatcctgc ctgatatggt aaggggcggc ttgccgtcat
      421 ttgaagaaca ttttgcccca agtgattcta gcgcatccag cagcggcttc atgggtcttt
      481 ttttgaggct ctcgtctcct gtcaggacag ttttgccttc ccttaaggcc gcaactgcag
      541 ctgcaattcg gatagatgtt ccagaatttg atgcgtcgac tatgaggtct gttgttccaa
      601 atttgtcgat tcctatcacc tcgagattat tttcattccc ctgaatttct gccccaaaac
      661 tcttgcagat ttcaattgtc gcattggtgt ctcttgagcg cagaacgttt ttgatgagac
      721 ttttgccgtt tgccaaagac gccaaaaaga ttgccctgtg cgtgtagctt ttgttcgggg
      781 ggcacgtaat tgtcccgcta agacttgatt tctctaccct acagttcatg gacctcagcc
      841 tttttgttgt ttatctcaga tgtgagaatt tttccctcgc tttccgaaaa cacctttctt
      901 atgtgtggta ttctgtcttt ttttgcaatg gctgcaatgg ctgggccgtt gcctgagatg
      961 gaagcgccaa gggcgccgct ttctactagt cttgaaatta tctttgggtc tgaattcaga
     1021 actgaggatg cggcaattcc atttaggatc atggcgtccc aataatttcc atcctttgca
     1081 aaactccacg ccctctcaaa tacgtccttg atggttctca gctgcctgat gtttcctcgc
     1141 ttgcgggact tggggacaaa aattacaaca gcgaggtttg tcggggtttt ttccattctg
     1201 attattttgc gctcgtagtt gttggtgact agtgtgcctc caaaataaca ggcacatgcg
     1261 tcatcatatg ctccagtcaa gctcaccttt gtggcaattg atgcgtcaac gccggcaagc
     1321 aagacctgcc tgtctgtaaa atgtgatctg aataacttgt ggcatgcaag cgacactgca
     1381 gaagagatgg cacttgagct cttcagacca tatcctgtcg gaatttcgga tttgagacta
     1441 atctcgattt tatttttttc aagatctttt tttgggactg cctcctcgac tactttgttg
     1501 atcaatctgg agctgatgtt ctggctgtct acctcaatcc ggattccgct gccttctgtt
     1561 gtgctgacta gtgcctccac cgtcgatgaa atcccaagtg ttgcaccttt cccagttgca
     1621 attgcgttga caattgatat tgcgccatga attgttgctt ttacctctgt catcaaaacc
     1681 caccgactag aacttttttc attgcgtcgt atggcgcctt tatgccgtgc caaatctcaa
     1741 aggccaaggc agcctggccc aaaagcatct catatccgta tatgactcgg gcagaattct
     1801 ttttgctttg aactattagg tccgtgttca tcggcatgta tatgatatcg tagacaatgt
     1861 tttctgaatt tatcgtcttt gtggaaatgg ggcttggctc gttttttagc ccaactgatg
     1921 tggcattgac gatgaacttg tagcttccag ccctttggcc tgcctcgtct agcgaaactg
     1981 cgtcagactc aagcccaagg cctgctgcaa atttggcaag ctcttggcct ttttcctttg
     2041 ttctgtttgc gattgtgatt ttgtgggcgt tttcctttgc aaatcctgca ataattgccc
     2101 ttgcggcgcc acccgcgcca ataagcaaga cactgcttcc attaactggt agatttcttt
     2161 ttttgattgg atccaaaaat ccgtccatgt ctgtgttgta tccgatgagc ctcccatcat
     2221 tattggtgac tgtgtttact gcgccaatga ttttgcattc ctggcttggc tcgtctaaaa
     2281 acttcatcat ctcgattttg tggggaatcg tgacgttaaa tccggtaatc ttgatttgtt
     2341 ttagcgactc gactccggcc tttagctcac cgtgtggaat cctgtatgcg atgtatgtgc
     2401 agtcaagccc cagcgccttg tacgcggcat tgtgaatgtt tggcgacagg gaatgatcta
     2461 ttgggtcgcc aattactgca aacatctttg tcattgttat ttttgcaatg caaagagcga
     2521 tttaacttca tctaagctaa actggcctgg cgcgacaggt ctgcccagag acacgtaggt
     2581 gtacggactg ccaagataca agcataggat tcttgagatc ctaccaaaat cccccatcgc
     2641 aaatgcaaca agattagagt tttgttttct atacaacgac aaaacagttg tagtgtccac
     2701 aaccgagctt gcagtggtaa ctattttgac gtgttttgaa atttttttca tctgctggag
     2761 tttttctgca agtctggaaa gcggcggagt tttcctaaaa tcgtgccacg agacaagaat
     2821 tggtgttcct gtttttctta catagtttag cagctctttg tttttgccaa gtgtggaata
     2881 ttctatgtct attagatatg ggttgtactc tgcaatcagc tttagaatgg atgcccgttc
     2941 tgcctcattt ccccggaacc tgcccccctc ggcttttggc ctgagcgtac agacacaccg
     3001 ccttaggtgt tttttgacta gttggagtgc ctcgggaact gcctctggcc tcaaaaagtc
     3061 aaagcggatt tcagcgtact ctgatttttt gagtgcatcg tatagtgtct ctttgagtcg
     3121 tctgggagtt gtttctgtaa tgctcacaca tgttttgtat gccatgtggt ccagtctatt
     3181 tttccaaaat atactcgtca gagacttcca tgccaaaatg tcttccagta gctgtctttg
     3241 cgtgaacaag tacagtgtct ccttttttga ggtgtgttac cgaaacgagg tgtcctcccg
     3301 gccttacaaa tcttattgtt tctgcgtcct gcgcaataat tccaccgacc tcatcgcctg
     3361 tctgggcctt tatcatgagc atcggccttc tttctatttt tgagcggcca acagttgctc
     3421 gtcttgctct tccgtgcgaa tcaatcacta ggacctctgc acccgtctct aactccgaca
     3481 gatattttgt cgtgccgtca ggtgatagcg tgtagcagtg tactgcacct gcgtttactc
     3541 gaaacggtct tggtgatgta aatgaagagc cgacagactc attgtggacc aaaaacaaaa
     3601 agtttgatct gcttccaatt agcatccctt cgcctttgtg tagcatcgat gccgtgtcaa
     3661 cgcagactcg ctcgccgtcg ccaacctctt tgatgtcaat tatctttgcc tctcttagct
     3721 caaagttgct tgtccctagg tacaccattg cctcccttgc ttcgctaatt gagcttgcct
     3781 caaagattac accgtccaca ccaatttcta agattgaaaa catttttcgc acctcttctg
     3841 gggttcgcgc aagtgcaaag atttcagtgt gcattttgtg aagctttgca atgatgttct
     3901 caaggggaat tatcttccag tcctttacct caattatgac aaaatcaagg cctttttttg
     3961 cctcaacaaa gattttctca atatctgaat ttgatagtac cttgaatcgc cttccgacct
     4021 ttttgccctt tggccttggt gcatcttttt ccaaaaccac gtatttggcg tttggcgatg
     4081 tgtaaattga ggcaatcttt gacttgccaa ggagttttgg atccagatag acggttttga
     4141 ccccctcgcc ttccagtcct gccaaaaact tgccaagctg cgacttggga acctttggta
     4201 aaattactag ctctttactt ttttccaatt ttcttcgctg cttcttttgc cgattctttt
     4261 ctaaagatta tcgcacccaa ggcctcaacc atctgggctg ggtttttgtg tgcaaaaatg
     4321 ttacgaccat aagttacacc tttagctccg gccttcattg cgtcctcagt catctgtaaa
     4381 atgtcggaat ctgtttttgc cttggggccg cctgcaatta ctattggaac tggagtgctc
     4441 tttacgattt ttgcaaacga gtcgacatca cctgtgtaca gtgtctttac aatgtctgct
     4501 ccaagctctg ccccaatcct tgctacgtgg ccgacgattt ctggatcgtg tgggtttttg
     4561 atgttttcac cgcgagggta catcattgcc aatagcggca tgttccactg gtggcacccc
     4621 tctgcaattt ttccaagctg ctcaagcatc tctggctctt cttttccacc aatgttgatg
     4681 tggagtgaga cgccgtctgc tccaagcctt agggcctcct caacactacc tgtgagcatt
     4741 tttctgtttg gcgcagtaga taatgtcgtg cttgcagaaa aatgaaccag cactcctaca
     4801 cggcttggcc ttggcaatga ctttagaatt cctttgttaa ttatgacgct ggtaaggccg
     4861 cgcaattcac actgctgaat aatttcagct ggactttcca atccatcaat tggaccattt
     4921 gaaattccgt gatccattgg gatgcagagc atctttcctt tgcgaagaat ccgctccatt
     4981 cgaatttgta ggcctgtgac catgggtgat ctgctttgtg ggccctaatt aaaaataacg
     5041 tggtgctgtc atctcaataa ctgagcgata acgccaaaac aatccattaa ggataaataa
     5101 taatgaaaaa ccacaaaaac cgagtcaatt gagccaaatc actgaacaaa tccattcaag
     5161 cgatgttggc gatattctgg aaaattcttt actcggaaat cgtccatcat acggggatac
     5221 agtacggctc ctcaaatcca acgatgtgaa cctgatgggt ctggtggccg gacatatcac
     5281 aaaaaagcga tttggcaaaa aggcctcgtt tgtaaacaat ataattttga attacaccaa
     5341 cgtttgcatt actgattgca agttctgcgc attttaccga ccgccaggag atgacgagtc
     5401 atacaccctg agccttgatc agatcgaagc aagagtaaag accgcatggg agatgtttgg
     5461 gattcgccaa gttttgatcc agggaggcca caacccaaac ctcggaatag aatactatga
     5521 ggacgcgttt aggatgatcc gaaaaaaata tccactagtc ggagtacacg ggctttccgc
     5581 atcagagatt gacatgattg caaaaataga aaaaacatca acaaaagagg tcttgctgcg
     5641 actaaaggag gcaggcctcc agtcggtgcc cggtgctgga gcggaaattc tagttgactc
     5701 tgtaaaagaa ataatcagcc caaaaaagat ctcaagtgat gagtggatta gaattatggg
     5761 cgaagcacac gcaattgga
//
LOCUS       DASWKH010000076         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2445538, whole genome shotgun sequence.
ACCESSION   DASWKH010000076 DASWKH010000000
VERSION     DASWKH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445538"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGA92_05380"
     CDS             complement(<1..296)
                     /locus_tag="VGA92_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9845879.1"
                     /translation="MKILLISPTLKGIGGIAQHARDLIKFLKDSGHQVDVISSENVPT
                     IPFKKFKNPSFLISSFLKAKFMKNYDIVHALHPVAALAMKNISGKKILTIHGVF"
     gene            500..733
                     /locus_tag="VGA92_05385"
     CDS             500..733
                     /locus_tag="VGA92_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HEX9845880.1"
                     /translation="MPTRSLTIKEEAYRALKAKKKEGESFTDVILRLTENKGSARRLL
                     EMMDALHSPELADSVEAASKQFRKNFKTRDVKI"
     gene            735..1118
                     /locus_tag="VGA92_05390"
     CDS             735..1118
                     /locus_tag="VGA92_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9845881.1"
                     /translation="MVCLDTDFLIAYLRNDSVARIKLEELDMKEEPLYTTAINAFEIY
                     KGAYNAKNPSKELVRVNNLLDAFYILMMDRESAKATGVIYNKSQPIGEADLLIAGITI
                     SNKQILLTRNKKHFSQISGLKTEIW"
     gene            complement(1131..>2618)
                     /locus_tag="VGA92_05395"
     CDS             complement(1131..>2618)
                     /locus_tag="VGA92_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX9845882.1"
                     /translation="LYFGSYEMYKKFSTYWADGKGAHAPNVMIEKCSCPNNCGLCSNH
                     LSHSGLANMIVTNRCDLTCWYCFFYVKKGLEGAYMYEPSHDQVRAMMKTLRAERPIAG
                     NSIQITGGEPMLRDDISDVIKIMKEEGVDHVQMNTNGIRHAMDPEAAREVRLAGCNNL
                     YLSFDGVTARTNPKNHWEIPYALDSCRKTGTTVVFVPTVIKSINDHELGGIIRYAQKN
                     MDVVHAVNFQPVSLTGRMGKTEREKYRITIPDCIQRIEEQTKGEVTVDDWFPVPSCMP
                     LTNVIEAFSSKPKYELSIHFACGAGTYVFEDAETKKFVPLPRFADIQGMLELFEDKAE
                     EIRSGKNKYFTMLEVVRKLSSFIDKKKQPAGLDLAKMFGNILMKRSFDSVGSWHVKGL
                     FLGMMHFQDKYNEDLERLQRCDIHYLTPDLRVVPFCAFNVIPEWYRDRIQKKYSITVE
                     EWEEREGVKLEDGLYRGLMRRGAGDELAAGCAKSQMFHQAAQAVV"
BASE COUNT          710 a          552 c          538 g          818 t
ORIGIN      
        1 ctaaaaaccc catgtatggt aagaattttt tttccagaaa tgtttttcat tgcgagcgca
       61 gccactggat gcagcgcatg aactatatca taatttttca taaattttgc ttttaaaaaa
      121 gaggaaatta gaaaactggg atttttgaac tttttaaatg gaattgtagg tacgttttct
      181 gaggaaatta catcaacctg atgtccactg tctttgagaa attttatcaa atctcgcgca
      241 tgctgagcta ttccacctat cccctttaat gtgggcgata tgaggagaat tttcataaga
      301 cgaaattagt tgtagaactt ttaaggtatt ggatcaaaaa atattttcta cattactgcc
      361 tgtgctgcct gatggaacgt ctggccattt cgggattata ttaaaggcac agaacggaac
      421 gactctagtt tcatgagact atcaaaatgt atcatattga attgtaaagt taataataca
      481 cgtacgtgta ttattaacta tgccaacaag gtctctaaca ataaaggagg aagcatatcg
      541 tgcactcaaa gccaagaaga aggaaggcga atcatttact gatgtcatac tccgactaac
      601 cgaaaacaaa ggcagtgcac gcagattgct tgaaatgatg gatgcactcc attcaccaga
      661 gttggcagac agtgttgagg ccgcaagcaa gcaatttcgt aaaaatttca agactcgtga
      721 tgtgaagatc tgagttggtt tgcctagata ccgacttttt gattgcatat ctgagaaatg
      781 attctgttgc aagaatcaag ttggaagagt tggatatgaa agaagagccg ctttacacca
      841 cagcaataaa cgcttttgag atctacaaag gtgcttacaa tgcaaagaat ccttcaaaag
      901 aacttgtacg agtcaataat cttctagatg ctttttacat actgatgatg gacagagaat
      961 cggcaaaagc gacaggagtt atttacaaca aatctcaacc cataggtgaa gctgatcttc
     1021 tgattgcagg cattacaatt tcaaataaac agattcttct tactaggaac aaaaagcact
     1081 tcagtcaaat ttcaggactc aaaacagaaa tctggtaaaa aatttctaga ttacactact
     1141 gcttgtgctg cctgatggaa catctggctt tttgcacagc ctgctgcaag ctcgtcgcct
     1201 gctccccttc tcattaatcc cctgtaaagg ccgtcttcta gtttgactcc ttcgcgttct
     1261 tcccattcct ctacagtaat ggagtatttc ttttggattc tgtcacggta ccattcaggt
     1321 atgacattga atgcacagaa tggaacgact ctgaggtctg gtgtcagata gtggatatca
     1381 catctttgca gcctttctag gtcctcgttg tacttgtctt ggaagtgcat cattcctagg
     1441 aataatccct tgacatgcca agagccaact gaatcaaatg accttttcat cagaatgttg
     1501 ccaaacatct ttgccaggtc cagtcctgct ggctgtttct ttttatcaat aaagcttgag
     1561 agttttctta ccacctctag cattgtaaag tacttgtttt tgccggatcg aatctcttct
     1621 gccttgtctt cgaataactc cagcattccc tgaatgtctg caaatcttgg tagcgggaca
     1681 aactttttgg tctctgcgtc ttcgaagaca tatgtgcctg caccgcaggc aaagtggatt
     1741 gataactcgt attttggctt gcttgagaac gcctcaatta cgtttgttag tggcatgcag
     1801 cttgggactg ggaaccagtc atctactgtg acttcgcctt tagtctgctc ttcaattctt
     1861 tggatgcagt caggaattgt gattctgtat ttttcgcgct ctgtcttgcc cattctgcct
     1921 gtaagtgata ctggctggaa gttgactgcg tgtactacat ccatgttctt ttgggcatat
     1981 cgaataattc cgcctagttc gtgatcatta attgatttga ttacagttgg aacgaataca
     2041 acagtagttc ctgtctttct gcaggaatcc agtgcatatg gaatctccca gtggttcttt
     2101 gggtttgttc ttgcagtaac accgtcaaaa ctcaaataga gattgttgca tcctgcaagc
     2161 cttacctctc ttgctgcttc tgggtccatt gcatgtctga tgccgtttgt gttcatctgg
     2221 acatggtcga ctccttcttc tttcattatc ttgattacat cggaaatatc gtcacgcagc
     2281 atcggctcgc ctcctgttat ctggatagag ttgccagcaa ttggtcgctc tgctctgagt
     2341 gtcttcatca ttgcacgaac ctggtcgtgt gatggctcgt acatgtatgc gccctcaagg
     2401 cccttcttta cataaaagaa gcaataccag catgtcaaat cacacctgtt tgtgacaatc
     2461 atgtttgcaa ggccgctgtg tgataggtgg tttgagcaca aaccgcagtt gttaggacat
     2521 gagcatttct caatcattac gtttggagca tgggcacctt tgccgtctgc ccaataagtg
     2581 ctgaatttct tgtacatctc ataagagcca aaatacag
//
LOCUS       DASWKH010000077        25349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2070999, whole genome shotgun sequence.
ACCESSION   DASWKH010000077 DASWKH010000000
VERSION     DASWKH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 25349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25349
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070999"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VGA92_05400"
     CDS             <1..787
                     /locus_tag="VGA92_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX9845883.1"
                     /translation="SQGITPCLATILVGDNQASATYVRNKQKACAEVGILTKDHTMPS
                     TFSQDEMNKLVDSLNSDESVHGILIQLPLPKQLDEFVTTSRISPLKDVDGLTPHNVGL
                     LVMKKAILRACTPSGIIELFDYYKIDLEGKNIVIINRSNLVGKPLYHLLLERNATVTM
                     CHSKTKNIQDVCRRADIVITGVGNRARFTLTPDMISDGAIVIDVATTRVDSKLAGDSD
                     FDKIIQKASYASPVPGGVGPMTIAMLLKNTVTAASLSKSFGRK"
     gene            774..1334
                     /locus_tag="VGA92_05405"
     CDS             774..1334
                     /locus_tag="VGA92_05405"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX9845884.1"
                     /translation="MEENNEKSSLRKLLLGRRDNLSADFMSIASKQIRKNLKKIEAYR
                     EAQTIACYYAIGSEVKTQDIIQEIISEGKTLALPKIDNGMLVFYNVKKFEDLEEGEFG
                     IMEPKQNCSVVNNFDIILVPAVAMTRKGQRLGYGKGFYDRFLAGKNITSVALTYSRLI
                     VKNIPRSNTDVPIHWVVTEDQVITAS"
     gene            complement(1329..2594)
                     /gene="purD"
                     /locus_tag="VGA92_05410"
     CDS             complement(1329..2594)
                     /gene="purD"
                     /locus_tag="VGA92_05410"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9845885.1"
                     /translation="MANVLVVGSGGREHALGWKLSQSPKVDKVFFAPGNGGTKDNIPI
                     QADDIENLADFAQENNCFTVVGPEAPLAAGIVDKFNQRKLKIFGPTKAAAQLESSKIW
                     AKTFMKKHGIPTSPFEVFDDEKKAKSYISSLDYDVVIKADGLAAGKGVIVCNGKQEAF
                     DAIDMMLVKNSFGSAGKKIIIEQRIDGIEASYIALTDGNVTVPMATSQDHKRIFDGDT
                     GPNTGGMGAYSPTGVISNDLAKKIHQDVIDKTIQSIKSEGITFQGFLYAGIMIKDGKP
                     YVLEFNARMGDPECQPILMRMGSDLYEYLDAAAEGKLKDKPPISWKEQSAVCVVLASK
                     GYPDSYPKNEEISGLDLANNDQVMVFHAGTKKDNGKVLTNGGRVLGVTALGKSLQEAI
                     TNAYLAVDKISWNNKYCRKDIGKKGLQYL"
     gene            2697..5891
                     /gene="ileS"
                     /locus_tag="VGA92_05415"
     CDS             2697..5891
                     /gene="ileS"
                     /locus_tag="VGA92_05415"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX9845886.1"
                     /translation="MQLEKEFDSKKIEDEVRAHLAKIDLQKMIWESKDQSKLVFIEGP
                     PTMNGIPHAGHLRGRVIKDLWYRYMTLRGHKIVFNAGWDTQGLPVELQAEKELGITGG
                     KTEILKSFGIEKLVDECKKLVHKYNEKWVAVDKLLAMSFNQKDAYWTYKDEFIEREWQ
                     FLKKAHETGILTEGYRVVAYCPSCQTSLSHAEVNQGYDMVQDPSLYYKVRLASEDAHL
                     IVWTTMPFTLVTDAMVGFNPDENYHYVKVQNETWIVGEKRLDEFMKEVKIESYEVIKT
                     VKGSEFEGKKYRHPLLDKIPKLKELAENPVVHLAVAESFVAADTGSGIVHLSPANGED
                     DYEIAQKRNVPIFNPINDEVKFTEDAGKYSGMFVRDSDRPIVEDLRDAGALVRIGRIK
                     HKYPLCWRSQHPIVWIARREFFYMLDRLGDKAIEAAENVEYFFEQPKNRFLGIIKEKH
                     PWCISRERFWGCPLPIWNCKGCGHIERLFSRKEIVDIASELPDGPNFELHRPWIDRIV
                     IKCKKCGAQMEREKFVLDTWHNSGSAPYSSLTDDEYKAGIPASFLTEGIDQTRGWAYT
                     LLIENVILNNKPISPFKSFLFQGHVLDKNGNKMSKSLGNVLDAQELLLKYPVDLIRFY
                     FIWKSSPIEPVNFSTDELMSRPYQILNTLYHIHLYFKQNSEYDKFDFAKTTLSWAQNK
                     NLFQPPDIWILSKLQKLVNEVQQNNDTCKLHEAARAIEDFLINSLSQVYIPITKAELW
                     DEESSKADRRLAIYAVLYEVLRTLDVLIHPLCPYTSEYLYLCTFGKDSILLESWPKYQ
                     SHLVNEPVEESFDLMKESVSVSAAARMKAKLKRRWPLKDAIICVKKSQKAKLEKLSEL
                     LLAQLNVESFRIVELESQDGLELVAEMQKLGLPAKPIVEMDRKKIGPKAKQHMNALLA
                     SFSSTKPEDIVTNLLKNGMHTFDVGQDKITLEREDFVVGFDAKEGYAFAQRDGFVVFI
                     STTRNREMMARGLIKDLARRLQTLRKERGYNPTDILQTASILELDEESLEMVKEKSSD
                     LAFLVRVKQIDFSGSGKNYKDDDIDGQKVRLSIE"
     gene            5949..6278
                     /locus_tag="VGA92_05420"
     CDS             5949..6278
                     /locus_tag="VGA92_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845887.1"
                     /translation="MVKQISLDAWQIQHLEDLLKKGSSIATKTGTPIVLYRQTLEEED
                     SSYEEIICTLTEKYVIEQLVISGGVVPPTFRQQMIYTLDEYPQRLVRKSKDLFLQTIE
                     LLEEQLS"
     gene            6311..7423
                     /locus_tag="VGA92_05425"
     CDS             6311..7423
                     /locus_tag="VGA92_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX9845888.1"
                     /translation="MRLLEYQAKELFAQYGIKTPKGIASKNIEQARKDAAQLGFPFVI
                     KAQVPVGGRGKAGGIQKCHNQDEFDLKYPQVLGMSIKGEKTRAILLEKMAEYEKELYL
                     SLFLNRSKRCYTIIASAEGGVEIESVKNQIIHEVGLGDVSPQIAEEVAKKMGLEGKTV
                     SDFTDMLQRLSKLTIEKEVELAEINPVALLKDGSLLALDGKVITDDNSNFRHPEMEKY
                     QEKSELEERAEKSGFTLVELDGNIAVVGNGAGLVMSTLDMLADNGGKPACFLDVGGGA
                     TTESVYEALTLISKMKKVKAILVNLYGGIVKTTTVASAFIKAYDDKLIDLPVYSRLMG
                     AESEKSKEMLKNTKTKMFDSVEDAINGVVMEVSKRG"
     gene            7416..8339
                     /locus_tag="VGA92_05430"
     CDS             7416..8339
                     /locus_tag="VGA92_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9845889.1"
                     /translation="MADIYEILRGQKDSSGNYQKVPVIVQGITGTFGSLHTKQMMDYG
                     TNIAAGVTPGKGGQTFEGKVPIYNTVKEAVAASDSKISILFVPAKFFLGAAKEALEAG
                     IKLLVAIPEHVPVRDTMLLLELAKQKDAIIIGPNTPGVMIPGLIKIGIMPASPFRPGN
                     IAVLSKSGTLLYEISNTLTQASFGQLITIGVGGDPINGTRLIDAFDMVKDDPNLHGMV
                     IVGEIGGDSEEILAQYIIDINFKKPIVAYVAGRRAPKEKRMGHAGAIVMGTYGSAESK
                     ISMFNKANIPVAKRPNEIAMLLAGKLEKPKD"
     gene            8368..8535
                     /locus_tag="VGA92_05435"
     CDS             8368..8535
                     /locus_tag="VGA92_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HEX9845890.1"
                     /translation="MPITDPEKKRIAQAARLHMKICLNCGGRNSLAATRCRRCHGNFL
                     RLKNRTLGAKK"
     gene            complement(8536..9513)
                     /locus_tag="VGA92_05440"
     CDS             complement(8536..9513)
                     /locus_tag="VGA92_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9845891.1"
                     /translation="MNDVILLWVLFSLFVGISLAIDLGVFSKLRRKKDQPEHVVPPFK
                     VALRWTITWISLAGIFAGIVYFDMGQEKLEEFVTGYALEYSLSVDNMFVFLLIFTSLN
                     IPHKFQHKVLSMGILGAVAMRIPLILVGVTLLETFHWMIYLFGAFLIFTALRMLAQRK
                     EKKIEVEKNIAIRVLRKFVPIEFELKDTRFFLRKDGILYATPLIVALVIIEFTDLLFA
                     LDSIPAILAITTDSFIVITSNVFAILGLRSLYFLLAGMMEKFYYLKPGLVTILLFIGT
                     KMIISEQVEIPTSLSLIVVLGVLGTALAFSFLRARKINSQNQDSNQNES"
     gene            complement(9603..10691)
                     /locus_tag="VGA92_05445"
     CDS             complement(9603..10691)
                     /locus_tag="VGA92_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9845892.1"
                     /translation="MQATELQQGKKFTLEEVVEFTDLIAEKLDAAIDKVDNINHKTHV
                     LSVNASIEAARAGTYGRPFGIVASNMSELSEETSKITEKMRSDTKEILNVGNLIKTQA
                     KDFRGNRLSDLALVNIDLIDRNLYERTADVRWWATDGNVVDALTKKTPDACDFASKRL
                     GVILQAYTVYNDLVLVDTSGNIVANGNTQEFSSVNTNVLNSPWFTSAMRTKSGSEFGF
                     QTVHKSPLVNNNLALVYSCAVREGGDTKGKIIGVLGVIFKWESLSQTIIQHTPIGEEE
                     KVNTRICIVDDDGLILADSENKMLRDEFQFDKKTSLFSEKKNYLFSEYEGAECCIAHA
                     LSPGFEGYSTGWHSLIIQKLGNRSKKSR"
     gene            complement(10853..11212)
                     /locus_tag="VGA92_05450"
     CDS             complement(10853..11212)
                     /locus_tag="VGA92_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845893.1"
                     /translation="MKDSLNILHSGSKQSEFGFIEQLGTCMQTIDALKQTTLSKKIQN
                     HKQIATILQEMASRIGSIQTDYYFRAGRTYFTINHGMGPAGNLFFKSVFENLLQDTKE
                     NSHFITQENNFCAIIRI"
     gene            complement(11301..11432)
                     /locus_tag="VGA92_05455"
     CDS             complement(11301..11432)
                     /locus_tag="VGA92_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845894.1"
                     /translation="MCARDHDDSFRRGRKKMVFDKKEDEFEAHFKTSMLFTKSMKSL"
     gene            11665..11973
                     /locus_tag="VGA92_05460"
     CDS             11665..11973
                     /locus_tag="VGA92_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9845895.1"
                     /translation="MKRIEAYFQFGRLETVVEAVEKAGAEGLTVVYARGRGSAERIPL
                     QSNRGTTVQRPLFNIIDGLVTIVEDHLVEPVVDAIKKNATHDAKGIIIVSDVHQVIKI
                     "
     gene            12069..13193
                     /locus_tag="VGA92_05465"
     CDS             12069..13193
                     /locus_tag="VGA92_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9845896.1"
                     /translation="MKVGVYHVQLIMYYDEVLTQSARNYAFTGDKKWELRYKAIEPLS
                     EQLLKDVIKNADDKTMQFFETMSLANMFSVKMECEALELINKGKKEDAIRLLEGDEYA
                     REKKSLTEGLEDFVAKMQLKQENDILSGILSPSVQQIVDLERKMAVLEDNVKKEKMIA
                     IGELAARLAHDIRNPLSIIKNSIDVLVVQNQNIDEKTLDTYKRIQRAVDRISYQIDDV
                     LNFVRPRMISLQQTSLNRILNSVINRLKLPNTVQVVLPKKDIEIVVDIVNIEIVFVNL
                     ITNALQAMDNTGRITIESDQDDIKTTIKISDTGPGIPADLLPQIFEPLFTTKQTGTGL
                     GLVSCKTIIEQHGGTLDAKSTQEGATFIITLPNHKIKTES"
     gene            13278..13562
                     /locus_tag="VGA92_05470"
     CDS             13278..13562
                     /locus_tag="VGA92_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845897.1"
                     /translation="MATKSSATVPKSKKSITLEKEIETKVRRIQARLIEKTDQNWSLS
                     TVLNILVTGGLLQTKKMSKNDWQKIRTLVEQKSISFDESTLMDFVKKIVG"
     gene            13643..16135
                     /locus_tag="VGA92_05475"
     CDS             13643..16135
                     /locus_tag="VGA92_05475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX9845898.1"
                     /translation="MKIPRVSSSIKAVELKQIPPPLLIGERLNTQGSKKAKQLVLNDD
                     FDGLLELARTQVEDGAHCLDVCVATTERSDELEFMKKLVKKLALEIDAPLVIDSTDPN
                     VIESIIEQIPGKPIINSINLEGDGSRFHRLAPLMAKYGIPAIALCIGPKGMAKTPQEK
                     LQTAELLIETGTKYGLREDQFIFDVLTFTLATGESEFLDAGKNTLEGIKLVKQRFPNS
                     FTVLGLSNISFGLSPAARKIINSVFLYHAVKAGLDAAIVNVREIVPYSEIDQKERNLA
                     EDLIFNTHPNALAELISYFEKVGTKITTEKKVEIDPSWSAGRRANFRIVNRLKDGIES
                     DVVVAIAEKISQKNILVEKDSRQTLNAPKEVTHPAAIQTLNDDLLPAMKEVGDKFGSG
                     ELILPFVLKSAECMKAAVVELEKYLLKEEGTSKGKFVLGTVYGDVHDIGKNLVKTIFE
                     NNGYTVYDLGKQVPLQKFIEKINEVKPDAVGLSALLVSTSKQMQHFVEHARQNNFEIP
                     ILCGGAAINSNYINRIAKDGGIYKSGVFYCKTMFEGLKTMDKIVSPERDQLISDWHVK
                     IEKWQETKVEKAEGAIPHSGIKPVTPPAVPVLNKPIRLRPSDIDLNEVWEYLSKKSLF
                     VLSWGLRGRGVDSNAEGEKLLKEWKKKVLDEKLFEPQAVYGYFVCHNKDGKIIVDNPN
                     GQKVVFDFPRSSQSKHLCLTDYFGEDDIVAFQSVTVGQKVQEIIDKWNKEDRYTDAYY
                     LHGLAVETAEALADLVNLRIKKELKLDGKRGLRYSWGYPSCPDVSQHHLVWKLLEPEK
                     SGMTLTEAGQIIPEQSTAAIVVHHPQAEYFVL"
     gene            complement(16138..17187)
                     /locus_tag="VGA92_05480"
     CDS             complement(16138..17187)
                     /locus_tag="VGA92_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845899.1"
                     /translation="MDWLIIAVAIILFIAVFLSIALAEAIDPPRRQEPPKWSFGEGLA
                     AGDFFEYQICDSILKIPESPNPCYVVTLEFLQLLPSPQGKMWIVAAHVDHQVRQVDMI
                     FQILEDSFKIKTDGTTIPYANSIEKTLGWIRQHASKFEPQTLAVGKTWGAIDSQTQGT
                     KTELLLTQVDSVQVNDKTYETYKVGYYLTKESFVQIKDGFPFPIKAAIYKPVSAYQNI
                     PLVFTVDLLSYSHSDLCNPAVIDPSLIPTNNKIQPQIHNKTLADTSQYDKLLDDDSDY
                     EFDIISTETEIKEIESYDETKDYENDTFENLEGALSDDYETLDDRVFSNFTRLLQALT
                     EAVNQIVSNQTTNEK"
     gene            17337..20198
                     /locus_tag="VGA92_05485"
     CDS             17337..20198
                     /locus_tag="VGA92_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845900.1"
                     /translation="MLGVLFLSSHAGYGQEAESGAHIWVPSEIITGENYEGLVVLDEA
                     SRSGQVIVLSSSDPTIIQIPESVTIPPHSNHGIFSIKPSRQGAVEIFAVADGKIISTN
                     LFVYSSSRQPEGLKIVLPVNSTKTETMIGYVLSVDAKDSPAPVTKDTVVTLSSTPMIE
                     FESSKLEIKSGKYYAKFIAKIKGSGKIYANSENLTVGEHAVTKIQDVVSIRVAVAPNI
                     IMENSKAYFFVWLEKDDRPYKPPYVIHAYLSSSNLKSIRFNENPQITQNSDSILRISL
                     VDGVGTGYLVSGDSGVSVITANVEGFGSAQTNVVVGPVLIDENFEFVEPDSNDKIGQI
                     ASKKPNVAFAWFYPEITDSKAYGVVALYNTNFTQTSSATISSNDTAVAVSSTINHVMP
                     VPLDGRTITLSSSSGLNHPNMLALTETNEILLKRGIGSTHAAQFEVFGVNQGNYTIFI
                     SGPGLEMFQSELSVASSFRDSYRLKLTLIPSIFGAKNDLAMISIVDDSESLINAQKMF
                     AGPLKVSVFSGNEQSEISISSLNSAVYSGTLNENAYVVASANGLSPIEQKIKPSGVAS
                     SVILDVPQKIHISEPFPYTIHEVDSFGIPIRKLNFTGISSTPGIITDGSYLRIDGIGY
                     ESIAAVTKNGADSKQINSFANTFSFSIVANGITNRIDKEFELRLNSDVEDYQVFVDSP
                     IPYKKIDETTYLILPNTEGHHTITFTAMKHGYAPSRNSFSVFAEKFVNLAIKATASDG
                     AELNVGQVIIIGNLTKSIVTPHQEEVRPQFLQTSFPADFVVGSKGYQLESVTFEDQKI
                     TDGKISNIFLNKNTEITASYQRMVKIDVENAQGSGFYPYGQTVVLSVAPKDRFFFLIR
                     DVFDHWEGLEYTSDHIAFAATHDVKAKAVLREDYTILSLIISGGASLFLYNNFVRKRG
                     LNVWFYFERLNLPSFGKILKLRAKKPTPKWQPDDSDYGF"
     gene            20351..20635
                     /locus_tag="VGA92_05490"
     CDS             20351..20635
                     /locus_tag="VGA92_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845901.1"
                     /translation="MGLEDSKKTMLIYVIENVLLKIGRSSYEIVLTRLQKDYGCHITE
                     CVERPEYLKKALEDIYGDASGEILEQIKTELGDLVEQKYYRNFIEVINAH"
     gene            complement(20665..21678)
                     /locus_tag="VGA92_05495"
     CDS             complement(20665..21678)
                     /locus_tag="VGA92_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9845902.1"
                     /translation="MDALDKEILNEIQWSFPLVTEPYKELARRFKISDDEMKKRILQL
                     KSVGVLRQLSAIFDTRRLGYKSSLVAMAIRPDKLDSIANLINRHPGVSHNYERNHEYN
                     LWFTLATPPGTDLKTEVDKFAKLDGITKIRLLPTIKLFKIGVKLDMVDEKKHEVAPTE
                     QKKEVKEIKFNPTEEDKEFIRQLQKDLEVTDRPFLSAAQSLGMSEDLVFEKLRYYEEI
                     GVMRRFAAILRHRDAGFIANGMIVWRVPETRIEEVGATLGAFPQISHCYQRPVYSDWP
                     YNVFSMVHCKSIQEAEDMAKEIQGHIKVDDYRILFSSREFKKTRVEYFVEHEFNIEET
                     IPA"
     gene            21884..22150
                     /locus_tag="VGA92_05500"
     CDS             21884..22150
                     /locus_tag="VGA92_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845903.1"
                     /translation="MAEDIQYIKKQIKKEMNHLRDLYSKINRIEKKKEPENYRIKKLE
                     QELMRRNPNIEIDRELLALVGTEPYNPPSQDKEVVRRITAERYG"
     gene            22143..22568
                     /locus_tag="VGA92_05505"
     CDS             22143..22568
                     /locus_tag="VGA92_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9845904.1"
                     /translation="MASTRPNVFVDVNIFVDISEKRQGWNNSLEVIAGIRKEKYNGYI
                     SAFTVPILYFRRIRIEPDKEARNNVWKLINGHHIIELNESVLEQAKQDIKFKDYEDAI
                     QYYSAKTKCDVIVTRNKKDFPHKDVKVMNPEEFLNRYSL"
     gene            22792..22986
                     /locus_tag="VGA92_05510"
     CDS             22792..22986
                     /locus_tag="VGA92_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845905.1"
                     /translation="MSVKIKGIKTKIIKENDGSYFIACSALGVYSSGQNLQTVKKNYL
                     KALEIHLSVLREKATEDIVI"
     gene            complement(23135..23272)
                     /locus_tag="VGA92_05515"
     CDS             complement(23135..23272)
                     /locus_tag="VGA92_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845906.1"
                     /translation="MQKAHKEKLNPEIEKKVKLIESGKAKGKTYTPDEYLKHVNNVLD
                     E"
     gene            complement(23346..24062)
                     /locus_tag="VGA92_05520"
     CDS             complement(23346..24062)
                     /locus_tag="VGA92_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX9845907.1"
                     /translation="MEFQILKNPMSILEVHLKKGETITAEAGALVFMKGEIEVNTKMR
                     GGFLKTVKVSLLGQESFFVNHYTAKEEGCVLGLTGPPVGDIIEIPISSDSGFIVQSGA
                     YIASTPGVDIDTKWQGFTKGIFGSELFMLKTTGEGKVFCNAYGGIIQKQIASGEKIVL
                     DNYHLVALSLNSDYKVTKFGGLKSTILGGEGLVTEINGPATVYFQTKNLKELIDLLGV
                     RRPSETQGHNFNIGGFRLGT"
     gene            24167..24820
                     /locus_tag="VGA92_05525"
     CDS             24167..24820
                     /locus_tag="VGA92_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX9845908.1"
                     /translation="MIVDLNLKGRLVIVVGAGQEGLKKVNSLLTQDCEILVISDKASK
                     QILDFAKAGKVKFKKIKLQDADFLLGYEPILVMAATDDKELNRKIVQKAKKMRCYAYA
                     ADDPDVSDFAHPSVINIEDTVQIAISTGGRSPAMARKLKLRAEKLFRQIIDKEDIYQI
                     KLQKIARDAAKSQIPTVLGRKKFLYSVLNNKKIKQLLKEDNLPSAQREVMKILGEWK"
     gene            24817..>25349
                     /locus_tag="VGA92_05530"
     CDS             24817..>25349
                     /locus_tag="VGA92_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9845909.1"
                     /translation="MSDIINARVTFRRSPVHVLEKFTFKDLESAYESFRKHSGLREFV
                     ILQTCNRVEIFGSTDELNLEKIKKTWASLSGLEQSAFKENFETSEDVDVFEHLLKLTA
                     GLDSMVVGEEQILGQIKEAITTARQLKASGEHINTLFDKAIKIGTRVRNSTGINKGTI
                     SVGSMAVKLAEENIDDL"
BASE COUNT         7918 a         5137 c         5432 g         6862 t
ORIGIN      
        1 gtcacaggga ataacaccat gtcttgcaac catacttgtt ggcgacaatc aggcatctgc
       61 cacatacgtt aggaacaagc aaaaggcttg tgccgaagtt ggaatactaa ctaaggatca
      121 tacgatgccg tcaacttttt ctcaggatga gatgaataag cttgttgact cgttaaattc
      181 ggacgagtcg gttcatggaa ttttgattca gctgccgctt ccaaaacagc ttgatgagtt
      241 tgtcaccaca tcaagaatct cgccgttaaa ggacgtcgac ggtctgaccc cacataacgt
      301 aggacttttg gtaatgaaaa aagcaatact tcgagcgtgt acgccatctg gaataataga
      361 attatttgac tactacaaga ttgatttgga aggaaaaaac atagtaataa ttaatcgaag
      421 caatcttgtt ggaaagccgc tgtaccacct cttgcttgag cgcaacgcaa ctgtaactat
      481 gtgccactca aagacaaaaa acatccaaga cgtttgcagg agggcagata ttgtaattac
      541 cggcgttggc aacagagcta ggttcaccct gactcccgac atgataagcg atggtgcaat
      601 agtaattgat gttgcgacca cacgtgttga tagcaagctt gcaggcgatt cggattttga
      661 taaaattata caaaaagcat cctatgcatc gcctgttcct ggcggtgtgg gccctatgac
      721 catagcaatg cttttaaaaa atacagtaac tgcagcatca ctgagtaaga gttttggaag
      781 aaaataacga aaaatcaagc ctgcgcaagc ttttgcttgg acggcgcgac aacctatctg
      841 cggattttat gagcattgca agcaagcaga ttcgaaaaaa tctcaaaaaa atcgaggcat
      901 acagggaggc acagacaatt gcatgttact acgccattgg tagtgaagta aaaacgcaag
      961 atatcataca ggaaattata agtgagggaa agacgcttgc cttgccaaaa atagataatg
     1021 gcatgctggt tttttataat gtcaaaaaat tcgaagattt ggaagaaggc gagtttggaa
     1081 taatggagcc aaagcaaaac tgttctgttg tgaacaactt tgacatcata ctggttcctg
     1141 ccgttgccat gacacggaaa ggacaacggc ttggatacgg aaaggggttt tatgacaggt
     1201 ttttggcagg caaaaacatt acgtcggtgg cattaacata ctctagatta attgtcaaaa
     1261 acataccaag atcaaatact gatgttccaa ttcactgggt tgtaacagaa gaccaggtaa
     1321 ttacggcatc atagatactg aaggcctttt ttcccaatat cttttctgca gtacttgtta
     1381 ttccagctaa tcttgtctac tgccaaatat gcatttgtta ttgcttcttg tagtgacttg
     1441 ccaagtgcag tcacacctag gaccctgccg ccgtttgtga gaaccttgcc attgtctttt
     1501 tttgttcctg catgaaacac cataacctgg tcattgtttg ccagatcaag tcctgatatt
     1561 tcctcgtttt ttggatacga gtcaggataa ccctttgatg ccaaaaccac gcacacggca
     1621 ctctgttctt tccacgaaat cgggggcttg tcttttagtt ttccctctgc agccgcatca
     1681 aggtattcgt acagatcaga gcccatcctc atcaaaattg gctggcactc tggatcaccc
     1741 atacgtgcgt tgaactccag aacgtacggt ttgccatcct ttatcattat gccggcgtac
     1801 aaaaatccct gaaatgtaat tccttcagac tttattgatt ggattgtttt gtctatgacg
     1861 tcttggtgta ttttttttgc aagatcatta ctaatcacgc cggttggcga gtatgccccc
     1921 atcccacccg tgtttggccc cgtgtcacca tcaaaaattc ttttgtgatc ttggcttgtt
     1981 gccattggga cggtaacgtt tccatcagtt agtgcgatat atgatgcctc aattccgtca
     2041 attctctgtt ctatgattat tttttttccc gcgctgccaa aggaattctt tactagcatc
     2101 atatctattg cgtcaaacgc ctcttgtttt ccgttgcaga caatcacgcc ttttcctgcc
     2161 gcaagcccgt ctgcctttat tacaacatcg taatcaagcg agctgatgta ggattttgct
     2221 tttttttcat cgtcaaatac ttcaaatggc gaggtaggaa tcccgtgttt tttcatgaac
     2281 gttttggccc agattttact tgattcgagt tgggctgctg cctttgttgg gccaaagatt
     2341 ttcaattttc tttgattaaa tttgtcaact attcctgctg caagtggcgc ctctgggcca
     2401 acaactgtaa agcagttgtt ttcctgggca aaatctgcca gattctcaat atcgtctgct
     2461 tgaattggaa tgttatcttt ggtcccgccg tttcctggtg caaaaaagac cttgtcaact
     2521 tttggtgatt gagacaattt ccatcccagt gcgtgctccc gaccaccaga accaaccact
     2581 agaacatttg ccaacaaacc aagtcttgga cgctcgtata taatctaaat ttcgagctga
     2641 aatccaaatc aaaaatttgc ctaactttat aaaatgagat cggtacctca ttgatgttgc
     2701 agctagaaaa agaatttgat tcaaaaaaaa tcgaggatga ggtaagggca catcttgcaa
     2761 aaattgattt gcagaaaatg atctgggagt caaaagacca atcaaagctt gtcttcatag
     2821 agggaccccc caccatgaac ggaattcctc acgccggcca ccttcgtggt agagtaatca
     2881 aggacctttg gtatcgatac atgacgttac gaggacacaa gattgtcttt aatgcaggat
     2941 gggacacaca ggggcttcca gttgagctgc aggcagaaaa agagcttggc atcactggag
     3001 gcaaaactga gattctaaaa tcatttggga ttgaaaagct cgtcgatgaa tgcaagaaac
     3061 tagttcacaa gtacaatgaa aaatgggtag cagtcgacaa gcttcttgct atgtcgttta
     3121 accaaaaaga tgcatactgg acatacaaag acgaattcat cgagcgcgag tggcagttcc
     3181 taaaaaaagc gcatgagact gggatcctaa ctgagggata ccgggtcgtt gcatactgcc
     3241 caagctgcca gacatcgctt tcccatgcgg aagtaaacca aggctacgac atggtacaag
     3301 acccatcatt atactacaaa gtcagactag catcagaaga tgcccatctc attgtgtgga
     3361 ctacgatgcc atttactctg gtgactgacg caatggtggg ctttaatcct gatgaaaatt
     3421 atcattatgt caaagtacaa aacgaaacat ggattgttgg agaaaaaaga ttagatgaat
     3481 tcatgaagga agtaaaaata gaaagctacg aagtaattaa gactgtcaag ggctctgaat
     3541 ttgaaggcaa aaaataccgt cacccgctct tggacaagat tccaaagcta aaggagcttg
     3601 cagaaaaccc cgttgttcac cttgcagttg cagagtcctt tgttgctgca gacactggaa
     3661 gtggaattgt gcacctctcg ccagcaaacg gagaagacga ttacgagatt gcacaaaaaa
     3721 ggaacgtgcc aatttttaat cccataaacg acgaggtgaa attcaccgag gatgcgggca
     3781 agtactctgg catgtttgtt cgcgattctg atagacccat tgtagaagac ctcagagatg
     3841 ccggagccct ggtaagaatt gggagaatca agcacaagta cccactttgc tggcgctcac
     3901 agcatccaat agtttggatt gcaagaagag aattctttta catgctcgac aggttaggcg
     3961 ataaggcaat agaggctgca gaaaatgtag agtatttttt tgagcaacca aagaaccgat
     4021 ttttaggaat aataaaagaa aaacacccct ggtgcatatc gagagaaagg ttttggggct
     4081 gtcctctgcc catctggaac tgtaagggct gcggtcacat cgagaggctt ttttcaagaa
     4141 aggaaattgt ggatatagca tctgagctgc cagacgggcc aaactttgag ctgcacaggc
     4201 catggattga cagaatagtc ataaagtgca aaaaatgtgg cgcacaaatg gagcgagaaa
     4261 aatttgtcct agatacttgg cacaacagcg gctctgctcc atattcatct ctaacagatg
     4321 atgaatacaa ggctggaatt cccgcatcat ttcttacaga gggaatagac cagacaaggg
     4381 gctgggcgta cacattgtta attgaaaatg taattttgaa caacaagcca atttccccgt
     4441 tcaagtcatt tttgttccag ggccacgttc ttgataaaaa tggaaacaag atgagcaaaa
     4501 gcctaggaaa tgtacttgat gcgcaggaac tgctcttaaa atatccagtg gatctgatca
     4561 ggttttattt tatttggaaa tcaagtccca ttgagccagt caactttagc accgacgagc
     4621 tgatgtcaag gccgtatcag atcctaaaca cgctgtatca cattcatttg tatttcaaac
     4681 aaaacagtga atatgacaag tttgattttg ccaagacgac acttagttgg gcccagaaca
     4741 aaaacctctt tcagccacct gacatttgga tactatcaaa gcttcaaaaa cttgtaaacg
     4801 aagttcagca aaacaatgac acttgcaaac ttcatgaagc tgcaagagca atcgaagatt
     4861 ttctcataaa ctcgctaagc caggtgtaca ttccaataac aaaagcagag ctttgggatg
     4921 aggagagctc aaaggccgac agaagacttg caatatatgc ggttttgtat gaggtgttaa
     4981 ggacactaga tgttctgatt catccgctgt gcccgtacac cagtgaatac ctctatcttt
     5041 gcacgttcgg caaagacagc atcttacttg agagctggcc aaaataccaa tcacatcttg
     5101 ttaacgaacc agttgaggag tcctttgatt tgatgaaaga atctgtttct gtttctgctg
     5161 cagcaagaat gaaggctaag ttgaagagaa ggtggccact caaagatgca ataatttgtg
     5221 tcaaaaaatc acaaaaggcc aagctcgaaa aactctcaga gctgctttta gcgcagctta
     5281 atgttgagtc atttaggata gttgagcttg aaagccaaga cgggcttgag ctggtagcag
     5341 agatgcaaaa actaggcctc ccagcaaaac ccatagttga gatggataga aagaagattg
     5401 ggccaaaggc aaaacaacac atgaacgcct tgcttgctag cttctcaagc acaaaaccag
     5461 aagacattgt gactaatctg ctaaagaacg gcatgcacac ttttgatgtt gggcaagaca
     5521 agataaccct agagcgggaa gactttgtgg tgggctttga cgcaaaagaa ggctatgcct
     5581 ttgcacaaag agacggcttt gtagtattca tctcaactac aagaaaccgc gagatgatgg
     5641 cgcgtgggct cataaaggac ttggcaagac gccttcaaac gctgcgaaaa gaacgcggat
     5701 acaatccaac ggatattttg cagactgcct caattttaga gctagatgaa gaatctctgg
     5761 agatggtaaa ggaaaaatcc tcagatcttg catttttggt gcgcgtaaaa cagattgact
     5821 ttagcggaag cggtaagaat tacaaagacg acgacattga tggtcaaaaa gttcgacttt
     5881 ctatcgagta agaagctagc ttttaacaaa taacggatgt tatatatcaa aaaatgctat
     5941 actaatcgtt ggtaaagcag ataagcttgg atgcttggca gattcaacac ttagaagatc
     6001 tgctaaaaaa aggttcaagc attgcaacaa agactggaac gccgatcgta ttgtatcgac
     6061 aaacactaga agaagaagac agctcctatg aggagatcat ctgcacgctg actgaaaaat
     6121 atgtgattga gcagcttgtc atttccggag gagtggtgcc accgacattt cgacaacaaa
     6181 tgatctacac attggatgaa tatcctcaaa gactggttag aaaaagcaag gatttgtttt
     6241 tgcaaacaat tgagctttta gaggaacaat taagctaaca gaatataaga cacccaaaaa
     6301 aatccgatcc atgcgacttt tggaatacca agcaaaagaa ttgtttgcgc aatacggcat
     6361 caagactcca aaaggaatcg cctcaaaaaa catagaacag gcaagaaagg atgctgcgca
     6421 gctaggtttt ccatttgtaa tcaaggcaca agtaccggtc ggtggccgag gaaaggcagg
     6481 cggaatccaa aaatgccaca accaagacga gtttgatcta aagtaccccc aagttttggg
     6541 aatgtcaatc aagggcgaaa aaacacgagc aattcttctt gaaaaaatgg ccgaatatga
     6601 aaaagagctg tacctttctt tgtttttgaa tcgcagcaag cgttgttaca caattattgc
     6661 ctcagccgaa ggcggagtcg aaatagagtc agtcaaaaac cagataatcc acgaagtcgg
     6721 ccttggcgac gtatcgcccc aaatagcaga agaggtggca aaaaaaatgg gtcttgaggg
     6781 aaagaccgtt tctgatttta cagacatgct gcaaagatta tcaaaactta caattgaaaa
     6841 agaagtagag cttgcagaga taaacccagt agcactgcta aaggatggct cgctcctagc
     6901 acttgatgga aaggtgatca cagacgataa ttccaacttt agacatccag agatggaaaa
     6961 ataccaagaa aagtcagagc ttgaggaaag ggccgaaaaa agcggcttta cactagttga
     7021 gcttgatgga aacattgcag ttgtaggaaa cggtgcagga cttgtcatgt caacactcga
     7081 catgcttgca gacaacggtg gcaagccagc ttgctttttg gatgtaggtg gtggggccac
     7141 tacggagtct gtctatgagg cactcacctt gatttccaag atgaaaaagg taaaagcaat
     7201 tttggttaat ctgtatggcg gaattgtcaa gacaacaacg gttgcatctg cattcatcaa
     7261 ggcatatgat gacaagctaa tagatctgcc ggtatattca agactgatgg gtgcagagtc
     7321 tgaaaaatca aaagaaatgc tcaagaacac aaaaacaaaa atgtttgatt ctgtcgagga
     7381 tgcgataaat ggcgtcgtaa tggaggtcag caaacgtggc tgacatttac gaaatcctaa
     7441 gagggcaaaa agactctagt ggaaattacc aaaaagttcc agtaattgtt caaggaatca
     7501 caggtacctt tggctcgctg cacacaaaac aaatgatgga ttatggaaca aacatcgcag
     7561 ctggcgttac tccaggaaaa ggagggcaga cgtttgaggg caaggttcca atctacaaca
     7621 cagtaaaaga ggcagtggct gcaagtgact caaagatttc aattttattt gtcccagcca
     7681 aattcttttt gggtgcagca aaagaggccc tcgaggcagg aataaagctt ctcgtagcaa
     7741 taccagagca cgtcccagta cgtgacacaa tgttgctact agagcttgca aagcaaaaag
     7801 acgccataat cataggtcca aacacaccag gcgttatgat cccaggcctg atcaaaatag
     7861 gaatcatgcc cgcaagcccc tttaggcctg gcaacattgc agtattgtca aaaagcggca
     7921 cattacttta tgaaatctca aacacactaa cccaggcaag ctttggtcag ctcatcacaa
     7981 ttggagttgg cggcgaccca ataaacggaa caagactcat tgatgcattt gatatggtaa
     8041 aggacgatcc aaacctgcac ggaatggtaa ttgttgggga gataggaggc gactctgaag
     8101 aaatactcgc acagtacata atcgacatta attttaaaaa accaattgtt gcatatgtcg
     8161 caggccggcg cgccccaaaa gaaaagagga tgggtcatgc cggggcaata gtgatgggaa
     8221 catatggttc tgcagaatca aaaatttcta tgtttaacaa ggcaaacatt ccagtagcca
     8281 aacgcccaaa cgagatagcc atgctcttgg ccggcaagct cgaaaaaccc aaagactaaa
     8341 aagtacatca aggggagtca aaaaaagatg ccaatcacag acccagaaaa gaagcgaatc
     8401 gcgcaagctg cgcgccttca catgaaaata tgtcttaatt gtgggggtag aaactcactg
     8461 gcagctacta gatgccgaag atgtcatggc aactttttga gactaaagaa cagaacgctt
     8521 ggcgccaaaa agtagctaac tttcgttctg attagagtcc tgattttggc tgttgatttt
     8581 tcttgctctc agaaaagaaa atgcaagagc tgtgccaagc acgccaagca ccactatcaa
     8641 tgatagagat gttggaatct cgacttgctc ggatattatc atcttggttc caataaagag
     8701 cagaattgtt actagtccgg gttttaggta gtagaatttt tccatcatac ctgccaaaag
     8761 gaaatagagg cttcgcaggc ccaagattgc aaagacgttt gatgttataa cgatgaaaga
     8821 atcagtagta attgccaata tagctggaat cgagtcaagt gcaaagagca gatcagtgaa
     8881 ctcgataatt acaagtgcca caatcagcgg tgtcgcatac aggattccgt cctttcttag
     8941 aaagaatctg gtgtccttta attcaaactc gattggaacg aactttcgca acacacgtat
     9001 tgcgatgttt ttttccacct cgatcttctt ttcctttctt tgagcaagca ttcgaagagc
     9061 cgtaaagata agaaatgcac caaagaggta gatcatccag tgaaacgtct caagtagtgt
     9121 cacacctacc agaatcaggg ggattctcat tgccactgcg cccaaaattc ccatcgaaag
     9181 caccttgtgt tggaacttgt gtggaatgtt aagtgacgta aagatgagca aaaacacgaa
     9241 catgttgtct actgaaagag aatactctag tgcgtatcca gttacgaatt cttcgagctt
     9301 ttcttgtccc atatcaaagt aaacaattcc tgcaaatatt cctgcaagcg agatccaagt
     9361 aattgtccat ctcagcgcaa ccttgaaggg cggaactaca tgctcgggct gatccttttt
     9421 tcgcctcagc tttgagaaaa cgccaaggtc aattgcaagc gatattccga caaataatga
     9481 aaaaagcacc caaagaagga ttacatcatt catgtgtcaa ttctgacaca tagtatacac
     9541 gtaatataca ttgtttgggc gcagattgtg cgtatgcgta ttacaatgag gggtgttttt
     9601 tgctagcggg attttttact tctgtttccc agcttttgga ttattagaga atgccaacca
     9661 gtggaatatc cttcaaagcc tggcgagaga gcatgcgcaa tacagcattc ggcaccctca
     9721 tactctgaga agaggtaatt ctttttttca gaaaagaggc ttgtcttttt gtcaaactga
     9781 aattcgtccc ttagcatctt gttttcagaa tcagcaagaa tcaaaccgtc atcatctacg
     9841 atgcagattc gtgtgttcac tttttcttcc tcgccgatcg gggtgtgttg aatgatggtt
     9901 tgtgataacg attcccattt gaatatgaca cctaagacac caatgatttt tccctttgta
     9961 tcgccgcctt ctctgacagc acacgagtaa accagtgcca agttgttgtt tacaagtgga
    10021 gacttgtgaa ctgtttgaaa accaaactcg cttccacttt ttgtcctcat ggcactcgta
    10081 aaccacgggg aattcaaaac gttggtgttt accgaagaaa actcttgtgt atttccattt
    10141 gccacaatat ttcccgatgt atcaactaga accaagtcgt tgtaaactgt gtatgcctgc
    10201 agtatgacac cgagtctttt tgatgcaaag tcacatgcgt ccggagtttt tttggttaac
    10261 gcgtcaacga cattgccgtc tgttgcccac caccgcacat ctgctgttct ctcatagagg
    10321 ttacggtcga ttagatcaat gttgactagt gccagatccg atagtctatt tccgcgaaaa
    10381 tccttggctt gtgttttaat cagattgccg acatttagta tttctttagt gtcgcttctc
    10441 attttctcag tgattttgga ggtttcctct gagagctccg acatgttcga tgccacgatt
    10501 ccaaatggcc gcccataagt tcctgcccgt gcagcctcaa ttgacgcatt tacggacaga
    10561 acgtgggtct tatggttaat gttgtcgact ttgtctattg cggcatcgag tttttctgca
    10621 ataaggtctg taaactcgac tacttcctca agtgtgaatt tttttccttg ctgtagctca
    10681 gttgcctgca cgtctttact tcttataaca tttcaataga acgctttgta ttgttgtttt
    10741 aaacaaacat cgcattttat agattgaatc cgtcatatac atcagtaaat aacaaaggca
    10801 acaattgcaa tacctattgc tttttggcag tagggtttgt ttttgttatt tgctagatcc
    10861 taataattgc acaaaagttg ttttcttgcg ttataaaatg tgaattttct tttgtatcct
    10921 gcaagaggtt ctcaaaaacc gatttgaaga acaggtttcc tgcaggcccc ataccatgat
    10981 taattgtaaa gtatgttcgt cccgccctaa aataataatc agtttggatc gagccgattc
    11041 ttgaggccat ctcctgcagg attgtggcaa tttgcttgtg gttttgaatt tttttgctca
    11101 acgtggtttg ttttagagca tcaattgttt gcatgcacgt acctaattgt tcaatgaatc
    11161 caaattctga ctgtttactg ccggaatgta gtatgtttag tgaatccttc aaactatact
    11221 agtcggattt tgtgcagatg tattagatca tgtgtgtttt taacacacat acaacaaatt
    11281 agttcgcagg cattgaggcg ctataatgat ttcatcgatt tggtaaaaag catcgaggtc
    11341 ttaaaatgag cctcaaattc atcctctttc ttatcaaaaa ccatcttttt tctacctctc
    11401 ctaaacgaat catcatgatc tcgcgcacac atgacattag tagaattatt ggcaatataa
    11461 aattttgaat gaatatgtgt gataaaaaaa taattttttg tttgcctaca gtaaaaaata
    11521 ttgtaaacaa aacatggcaa aatcagtttg caataaaaat ttgtttgata attcaaaaat
    11581 aaattacacg tagtgaaaaa ttttcttttc aatcttggca atagcctgaa tctttatatt
    11641 gataaaaaaa ggactcatct gaacatgaaa agaattgaag cgtattttca gtttggaagg
    11701 ttagaaacag tagtagaagc tgtagaaaaa gcaggggccg aaggcttaac agtagtttat
    11761 gcaaggggaa gaggatctgc ggaaagaata ccgctccaga gtaacagagg tacaaccgta
    11821 cagagaccac ttttcaacat catagatggt cttgttacca ttgtggaaga ccatcttgta
    11881 gaacctgtag ttgacgccat aaaaaaaaat gcaacccatg atgcaaaggg aatcataatt
    11941 gtctcagatg tccaccaagt tatcaaaatc taaaatcatt tgtttacagc tacgattctt
    12001 atacggtctt agacacaatg tggtatacag attttgctaa aaattaagat cgagttgggt
    12061 gcagaaaaat gaaagttggc gtatatcatg ttcagttgat tatgtactat gacgaagtgc
    12121 taactcagtc tgcaaggaat tatgcattta ctggtgacaa aaaatgggag ctacgttaca
    12181 aggcaattga gcctttgtca gaacaacttc tcaaagatgt aataaagaat gctgatgata
    12241 agacgatgca attttttgaa accatgagtt tggcaaacat gttttcagtg aaaatggaat
    12301 gtgaggccct tgagcttatc aacaagggca aaaaagaaga tgcaatcaga cttttagaag
    12361 gagacgaata tgccagagaa aaaaagtctc tcacagaagg actagaagat tttgttgcaa
    12421 aaatgcagct aaagcaggaa aatgacatac tgtccggcat actgagccca tcagttcagc
    12481 aaatagtgga tctagagcga aaaatggcag tacttgaaga caatgtaaaa aaagaaaaaa
    12541 tgatcgcaat aggtgagctt gcagcacggc ttgcacacga catacgcaat cccctatcca
    12601 ttatcaaaaa ctctattgac gtgcttgtcg tgcagaacca aaacatagac gaaaagacgc
    12661 ttgatacata caaaaggatt cagcgcgcag tagacagaat atcgtaccaa attgacgatg
    12721 ttttgaattt tgtcagacca agaatgattt cactgcaaca aacatcacta aatagaattt
    12781 tgaattctgt gataaatcgg ctcaagctgc caaatactgt gcaagtcgtt ttgccaaaaa
    12841 aggatattga aattgttgtc gatattgtca atattgagat cgtctttgtc aacctgataa
    12901 caaacgccct acaagcaatg gataacacag gccggataac tatagaatcc gaccaagacg
    12961 acatcaagac tacaattaag atttcggata ccggtccagg cattcctgca gatctattgc
    13021 ctcagatctt tgagccgctg tttactacaa agcaaacagg gactggactt gggcttgtaa
    13081 gctgcaagac aataattgag cagcatggtg ggacgcttga cgctaagagc acgcaagagg
    13141 gcgccacttt catcataact cttccaaacc acaaaataaa aacagaaagt tagtctagta
    13201 aggtaggaat gtgtgcagaa ttattattgc caagtttggc agaccagatt tttataactg
    13261 gatttagaaa ttagtttatg gccacaaaat catcagcaac agttccaaaa tcaaagaaga
    13321 gcatcacgct tgaaaaagaa attgagacaa aggtacgcag aattcaggca cgactaattg
    13381 aaaaaacaga tcagaactgg agcctttcta cggtcttgaa catacttgtg actggggggc
    13441 tgcttcaaac aaaaaagatg agcaaaaatg attggcagaa gatacgaacc ctagttgagc
    13501 aaaaatccat tagttttgac gaaagcacgc tgatggattt tgttaaaaaa attgtaggtt
    13561 aattctttat tgcacctaac aaaatggtca ataatagaaa aaccagtata tatttagaaa
    13621 atgccacatc aatttggtaa gattgaagat tccgcgcgta agctcatcaa taaaggcagt
    13681 ggagctaaaa caaatcccgc cgccactact aattggggaa aggcttaaca cgcaaggatc
    13741 aaaaaaagca aaacagcttg tactaaatga tgattttgac ggactgcttg agctggcaag
    13801 aactcaggtc gaggacggag cacactgtct cgatgtgtgt gttgcaacaa ctgaacgctc
    13861 agacgagcta gagtttatga aaaagctagt caaaaaactg gccctcgaaa tcgatgcgcc
    13921 gctcgtaatt gactctactg atcccaacgt aattgaatct ataatagaac agattcccgg
    13981 caaacccata atcaactcca tcaatctgga aggcgacgga agcaggtttc acagactggc
    14041 gccgctaatg gcaaaatatg ggattcctgc aatcgcactc tgcattggcc ccaagggaat
    14101 ggcaaagacc cctcaggaaa aactgcaaac agcggaactt ttgatagaga ctggaacaaa
    14161 atacggcctg cgagaagacc aattcatctt tgacgttctg acattcacgc ttgcgacagg
    14221 cgagtccgag tttttggatg ctggcaaaaa cacgcttgaa ggaatcaagc tagtcaagca
    14281 aagattcccg aattcattta ccgtcttggg cctaagcaac attagctttg gtctttcccc
    14341 tgccgcacgc aagattatca actcggtgtt cttgtatcat gcagtaaagg caggactaga
    14401 tgcggcaata gtcaacgtac gcgagatagt tccttattca gaaattgacc aaaaggagcg
    14461 caatcttgca gaggatttga tcttcaatac ccatccaaat gccctagccg aattaatctc
    14521 atattttgaa aaagtcggca caaaaataac tactgagaag aaagttgaga tcgaccctag
    14581 ctggagtgcc ggaagacgcg ccaatttcag aatagtaaac aggctcaaag acgggattga
    14641 aagcgatgtt gtcgttgcga ttgcagaaaa aatttcacaa aaaaacatac ttgtagaaaa
    14701 ggacagccgg caaactttga atgctccaaa ggaagtaacc caccccgctg caatccagac
    14761 attaaatgac gatctgcttc ctgcaatgaa ggaagtcggc gacaagtttg ggtcaggtga
    14821 gctcatcttg ccgtttgttt taaaatccgc agagtgtatg aaggccgcag tagtagagct
    14881 tgaaaaatac ctgctaaagg aagagggaac aagcaaagga aaatttgttt taggtacggt
    14941 atatggagac gttcatgaca ttgggaaaaa cttggtaaag acgatttttg aaaacaatgg
    15001 atacactgtt tatgatcttg gaaagcaggt tcccctgcag aaattcatcg aaaagataaa
    15061 cgaggtaaag cctgatgcag tcggcctctc agctcttcta gtctccacat caaagcagat
    15121 gcagcacttt gtagaacacg caaggcaaaa caactttgag atcccaattt tgtgtggcgg
    15181 tgccgcaata aactccaact atatcaacag aatcgcaaaa gacggcggca tttacaagtc
    15241 tggcgtgttt tactgcaaga caatgtttga gggcctaaaa acaatggaca aaattgtatc
    15301 cccagagcga gaccagctta tcagtgattg gcacgttaag attgaaaaat ggcaggagac
    15361 caaggtcgaa aaagcagaag gtgccatccc acattctggg ataaagccag ttacgccccc
    15421 ggctgtgcct gtcttgaaca agccaattag gctcagaccg tcagatattg atttgaacga
    15481 ggtatgggaa tatctatcca aaaagtcgct ctttgtgttg tcctggggcc tgcggggacg
    15541 cggagttgat tctaatgccg aaggcgaaaa gcttctcaaa gagtggaaga aaaaagtgct
    15601 agatgaaaaa ctctttgagc cgcaagctgt gtatgggtat tttgtctgtc ataacaagga
    15661 cggaaagatc atagttgata atccaaatgg gcaaaaagtt gtttttgatt ttccgcgctc
    15721 ctctcaaagc aagcacctat gtctgactga ttattttgga gaagacgaca ttgttgcatt
    15781 ccagtccgtg accgtaggac aaaaagtcca agaaataatc gacaaatgga ataaagaaga
    15841 caggtataca gatgcctact atctgcatgg tcttgcggta gagactgccg aggcgctagc
    15901 agatctggta aatcttcgaa tcaaaaaaga attaaaactt gatggaaaaa ggggcctaag
    15961 atacagctgg ggctatccaa gctgccccga tgtctcacag caccacctag tctggaagct
    16021 gctagagcca gaaaagtccg gcatgacgct aacggaggca ggccagataa ttccagagca
    16081 gtcaactgcg gcaattgtag tgcaccaccc acaggcagag tatttcgtgc tgtaagatta
    16141 tttttcattc gttgtttgat ttgacactat ctgattgaca gcctcagtaa gggcttgcaa
    16201 aagcctagta aagttactaa aaactcgatc atcaagggtt tcataatcat cgcttaatgc
    16261 accctccaag ttttcaaatg tatcattttc ataatctttg gtttcatcgt aactctcaat
    16321 ttccttgatt tctgtttctg tagatattat gtcaaattca tagtctgaat catcgtctag
    16381 taatttgtca tattgacttg tatcggcgag cgttttatta tgaatttgag gctggatttt
    16441 gttatttgtt ggaattaatg acggatcaat tactgctgga ttgcacaaat ccgagtgcga
    16501 gtaggaaaga aggtccactg tgaatacgag tggaatgttt tggtatgccg atacgggttt
    16561 gtatattgct gcttttattg gaaagggaaa cccgtctttg atttggacaa agctctcctt
    16621 tgttagataa tatccaacct tgtaggtctc gtaggtttta tcattgactt gaaccgaatc
    16681 gacttgggtt agcaaaagct ctgtcttggt accctgagtc tgagagtcga ttgcccccca
    16741 ggtcttacct acagctaggg tctgaggttc aaactttgat gcatgttgtc ttatccatcc
    16801 aagtgttttt tcaatggaat tagcgtatgg aattgtagtt ccatcggttt taatcttaaa
    16861 cgaatcttct aggatctgaa aaatcatgtc gacttggcgg acttgatggt ccacatgagc
    16921 tgcaacaatc cacatctttc cttgaggact aggaagcaat tgtagaaatt ccaaagtcac
    16981 gacataacat gggttgggag actctggaat ttttagaatc gagtcgcata tttggtattc
    17041 aaaaaaatcc ccagctgcga gtccctcgcc aaaactccac tttggtggtt cttgtctgcg
    17101 tggtggatca attgcttcag ctagggcaat agataagaaa actgcaataa acagaattat
    17161 cgctacggca attatgagcc aatccataat tttcgaagcg ggcgaattgt ttgaattcat
    17221 acaaattatg aaaacggaat ttttaagaca caagataagc ttgaagcaat cttgcctact
    17281 tgcttaaatt actagaaatt ttttagattg tgcgtgattt tggtgctttc tgtgctgtgt
    17341 taggcgtact tttccttagt tcccatgcag ggtatggcca agaagcagaa tctggcgcgc
    17401 acatttgggt gccaagtgaa atcataactg gtgaaaatta tgaaggacta gttgtcctag
    17461 atgaggcgtc taggtctgga caagtaatcg ttctttcgtc tagtgatcca accataattc
    17521 agattcccga aagcgttaca attccaccac attccaatca cgggattttt tcaataaagc
    17581 cgtcacggca gggagcagtc gagatttttg ccgtagctga tggaaaaata atctccacaa
    17641 acctttttgt ttattcctcc agcaggcagc cagaaggact aaaaatcgtg cttccggtaa
    17701 attcaaccaa aactgaaacg atgattggct atgtcttatc agttgacgca aaggattctc
    17761 ctgcgccggt aacaaaggac accgttgtca cactgtcatc cacgcccatg atagagtttg
    17821 aatcctcaaa gctggaaata aagtcaggaa aatactatgc caagttcatt gcaaagataa
    17881 aaggctctgg aaaaatttac gcaaattcgg aaaacctcac agtaggcgaa catgctgtta
    17941 ctaaaatcca agatgttgtg tccatcagag ttgccgtagc acccaacatc ataatggaaa
    18001 attccaaggc gtattttttt gtctggctgg aaaaagacga caggccatac aagcccccgt
    18061 atgtcattca cgcatatctc tcatcaagca atttaaaatc aatccgcttc aatgagaatc
    18121 cgcaaataac gcagaattcg gattccattc tgagaatttc tctagttgac ggtgtcggga
    18181 ccgggtatct ggtttcgggg gatagcggag tatcggtaat tactgcaaat gtagaaggtt
    18241 ttgggtctgc tcagacaaat gtagttgtag ggccggtgct aattgatgag aattttgagt
    18301 ttgtagagcc agacagtaat gacaagattg gccagattgc aagcaagaag ccaaacgttg
    18361 cgtttgcctg gttttatcct gaaatcaccg actctaaggc atatggcgta gttgccctgt
    18421 ataatacgaa ttttacgcag acatctagtg ctaccatatc ctcaaatgac accgcagtgg
    18481 ctgtatcaag cacaattaat cacgtgatgc cagttcccct agacggcaga accataacac
    18541 tgagctcatc gtctggccta aatcacccaa acatgcttgc cctcacggaa actaacgaga
    18601 tcctactaaa gcggggaatc ggctcaacac atgcggcaca gtttgaagtc tttggagtca
    18661 accaaggcaa ctatacaatt ttcatttccg gtcctggtct tgaaatgttt cagtctgaat
    18721 tgtccgtggc atcctcgttt cgtgattcgt acagactgaa gctgaccctc atcccatcta
    18781 tctttggcgc aaaaaatgat cttgcaatga tttcaattgt cgatgattct gaatcgctaa
    18841 ttaatgcgca gaaaatgttt gctgggcctc tgaaggtttc tgttttttca ggcaacgaac
    18901 aatccgagat tagcatctcg tcgctaaatt cggctgttta ttcaggaact ttaaatgaga
    18961 acgcatatgt ggtagcctct gcaaacggcc tatccccaat agagcaaaaa atcaaaccgt
    19021 ctggagttgc gtcttcagta atacttgatg taccacaaaa aatacacatc tcagagccat
    19081 tcccatatac catacatgag gtggattcat ttggaattcc aattagaaag ttaaatttca
    19141 caggcatttc ttcgactcct ggaatcatta ctgatggcag ttatttgcga attgacggca
    19201 taggatatga gagtattgct gcagtcacca agaacggggc agacagcaag caaatcaatt
    19261 cgtttgcaaa cacgtttagc ttctccattg ttgcaaatgg aatcacaaat agaattgata
    19321 aagagtttga gctaagactg aatagcgatg tagaagacta tcaggttttt gttgattcgc
    19381 caattccgta taaaaaaatc gacgagacta catatctgat tttgcccaac acagaaggcc
    19441 accacaccat cacctttact gcgatgaagc atggctatgc cccatctagg aattcctttt
    19501 ctgtttttgc cgagaaattt gtaaatctag ccataaaggc aacagcaagc gatggggccg
    19561 agctaaacgt aggccaagta atcataattg gcaacctaac aaaaagcata gttactccac
    19621 atcaagaaga ggtcaggccc cagtttctcc agactagctt tccagctgac tttgttgtcg
    19681 gcagcaaagg ctaccaatta gaatctgtta cttttgagga ccaaaagatt actgacggca
    19741 agataagcaa tatttttttg aataaaaaca cagagattac agcatcctac cagagaatgg
    19801 taaaaatcga tgttgaaaat gctcaggggt ctggatttta tccctatggg cagacggtgg
    19861 ttttgagcgt tgccccaaag gatcgatttt tctttctgat cagagacgta tttgaccatt
    19921 gggagggcct cgaatacaca tcagatcata tcgcctttgc tgcaacgcat gacgtcaaag
    19981 ccaaggcagt cttaagggaa gactatacaa ttcttagcct gatcatatcg ggcggagcct
    20041 cgttgttttt gtacaataat tttgtaagaa agcgcggact aaatgtgtgg ttttattttg
    20101 aaaggctgaa tcttccatcc tttggcaaaa ttctaaagct cagagccaaa aaacccacac
    20161 caaaatggca gccagacgac tctgactatg ggttttgatc cgtgtagaat aactcgaaaa
    20221 aaaaattgta ttattgtaac cagaatagtt ccgttttgga accataattc tcaataattg
    20281 ttcccaaaaa gaaatcagga gatggatatt tttccaccat actaaataga attggagata
    20341 ttagagcgtc gtgggactag aagattcaaa aaagacgatg ctcatttatg tcattgaaaa
    20401 tgtgctccta aagatcggta ggtcgtcata cgagattgtc ttaacaagac tgcaaaaaga
    20461 ctatggatgc cacatcactg agtgtgttga gaggcctgaa tacctcaaga aggccttaga
    20521 agacatttac ggagacgcct ctggtgagat tttagagcag ataaaaaccg agcttggcga
    20581 ccttgtggaa caaaaatact ataggaactt tatcgaggtc ataaacgctc actagcttga
    20641 atccggataa tttttcaaaa aatattatgc tggaatcgtt tcttctatgt taaattcgtg
    20701 ctctacgaaa tattctacgc gcgtcttttt gaattcgcgt gagctaaaca gaatcctata
    20761 gtcgtcaact ttgatgtggc cctgaatctc ttttgccatg tcttctgcct cttgaattga
    20821 cttgcagtgt accatggaaa agacattgta tggccagtcc gaatacacgg gcctctggta
    20881 gcagtggcta atctgtggaa atgccccaag tgttgcccca acctcttcta ttcttgtttc
    20941 tggtacgcgc cagacaatca ttccgtttgc gataaatccg gcatctcggt gtcttaggat
    21001 tgcggcaaat ctccgcatta cgccgatttc ttcatagtat cgtaattttt caaagactaa
    21061 gtcttcgctc atgccgaggc tttgcgctgc agacaggaac ggcctgtcag ttacctccag
    21121 gtctttttgg agctgtcgta tgaactcctt gtcttcttcg gttggattga acttgatttc
    21181 ctttacttct tttttttgct cagttggtgc gacctcgtgt tttttttcat caaccatgtc
    21241 aagctttact ccgattttga acagctttat tgtcggcaat aatcggatct ttgttatacc
    21301 atcaagcttg gcaaacttgt ccacctctgt cttaagatca gttccaggcg gcgtcgccaa
    21361 ggtaaaccag agattgtatt catggtttcg ctcatagttg tgagaaacgc ccggatgacg
    21421 gtttatcaga tttgcaatag agtccagctt gtctggcctt attgccattg caacaaggga
    21481 gcttttatac ccaagcctcc tcgtatcaaa gattgccgaa agctgtcgta aaactcctac
    21541 tgactttagt tgcaggattc tttttttcat ctcatcgtca gatattttga atcgccttgc
    21601 aagctccttg tacggctctg tgactagcgg aaatgaccac tggatctcat ttaggatttc
    21661 cttgtctagc gcatccatag tcatgtgttc ttagaattta cttttttatt aaaaatgtag
    21721 cgggcctagg ggttttagca cctctagtgg acctctaatg gttcaatttt tggttccgcg
    21781 gccagtatta gaaggtcagc tttaaaggtt tctaaaaatt gtttttgagt ggcgtataca
    21841 gccataaatt tatttaaatg agaatgacta tctaattatt cttatggcag aagacattca
    21901 atacatcaaa aaacagatta agaaagaaat gaatcacctc agagatctct atagcaagat
    21961 aaatcgaatt gaaaaaaaga aagaacctga aaactacaga attaagaaac tcgagcagga
    22021 gctaatgagg agaaatccta acatcgaaat agatcgagaa ttgttggcac tggtcggcac
    22081 cgagccatat aatccgcctt cccaagataa ggaagtagtg agacgcataa cggctgaacg
    22141 ttatggctag tacaaggccc aacgtttttg ttgatgtaaa catctttgtt gatatttctg
    22201 aaaagcgtca gggttggaac aacagccttg aggtgatagc tggtattaga aaagaaaaat
    22261 acaatggcta tatttcagca ttcaccgtcc cgatcctata ctttcgcagg atacgaatag
    22321 aaccagacaa agaagcaaga aacaatgtgt ggaaactcat caatggtcat cacataattg
    22381 agttgaatga atcagtacta gagcaagcca aacaggacat caaattcaaa gattacgaag
    22441 atgcaataca gtattactct gcaaaaacaa aatgtgatgt tattgttacc cgaaacaaaa
    22501 aggattttcc tcacaaggat gtcaaagtca tgaatcccga agagtttctg aataggtata
    22561 gcttgtaaaa gcctcaattg ttcccaaata attgcagctt ttacacaaat aaaacaccgt
    22621 tccatttgag tgactcacaa acttttgagg ttctgcattt ggacagttgg gccttctcaa
    22681 atcatgtcta gtgtccacgt tttacatata caaaatttgt ataaaaatat gctagtcatc
    22741 aagtacaaaa tgaggaaact tcaataatta aaacgagtat ttgactacca gatgtctgtt
    22801 aagataaagg gtatcaaaac taaaataatt aaagaaaacg atggttctta ctttattgca
    22861 tgttctgcat taggcgttta tagctcgggg caaaatctgc aaactgttaa aaaaaactac
    22921 cttaaggcgc ttgagattca tttgtcagta ttaagagaaa aagccaccga agatattgtt
    22981 atttgaaaag gctttcatcc acacaccgaa aaggatcaaa attctttgta acaaatttgg
    23041 ctttcaagca ataagacaaa aagacttggt ttgacttgaa tttctttagt ttcttgtcta
    23101 tagtgtcatc aaatggttga attagccaca aagcctattc atctaaaacg ttgttaacat
    23161 gttttagata ttcatctggc gtgtaagtct tgccctttgc tttaccagat tcaatcagtt
    23221 tcactttttt ttcaatttct gggtttagtt tttctttgtg tgctttctgc acatgtttga
    23281 aatagacaat tttctatata aatacaagta tggaatgagc agagaatgca cattagatga
    23341 aaatcttatg tacctaatct gaatccgccg atgttaaagt tatgtccctg ggtttcactg
    23401 ggtcgccgca ccccaagcaa gtcaattaac tctttgaggt tttttgtttg aaagtatact
    23461 gttgccggac cattaatttc agtgactagt ccttcgcctc caagaatcgt gcttttcagt
    23521 cccccaaact tggtcacctt gtagtcagag ttaagactca gtgcaacaag atgatagttg
    23581 tctagaacta ttttttctcc cgatgcaatc tgtttttgta ttattccgcc atatgcgtta
    23641 caaaatacct ttccttcccc ggttgtcttt agcatgaaaa gctcgcttcc aaatatcccc
    23701 tttgtgaagc cctgccattt tgtgtcgata tcaactccag gagtagatgc gatgtatgcg
    23761 ccagactgca caataaaacc agaatcacta ctaattggaa tttctattat atctccgact
    23821 ggtggacctg taagacccaa aacgcatcct tcttcttttg cagtataatg gttgacaaaa
    23881 aacgattctt gtcctaacaa tgaaaccttg actgtcttta gaaagccgcc gcgcatcttt
    23941 gtgtttacct cgatttctcc tttcatgaac accagcgcgc ctgcttctgc agtgattgtt
    24001 tcgccttttt ttaggtgaac ctcaagaatg ctcatcgggt ttttcagaat ttgaaattcc
    24061 acggattaga aagaaaataa gactagaaaa ccgttactat actctaatgg ttcgaagaaa
    24121 attcgagcta gaatcctata aatacaattt ttgagctgcc ctgatcgtga ttgtcgacct
    24181 caacctaaag ggcagattag tcattgtagt aggtgcaggc caggagggac tcaagaaggt
    24241 caactcgctt ctgacgcagg actgcgaaat tctggtaatt tctgacaagg caagcaagca
    24301 gattctagat tttgcaaaag ctggcaaagt aaaattcaaa aaaatcaagc tgcaagacgc
    24361 ggattttctt ttagggtacg agccgatttt ggtaatggct gcaaccgatg ataaggagct
    24421 gaatagaaag atagtacaaa aagcaaaaaa gatgcgctgc tatgcatatg cagcagacga
    24481 ccccgatgta agcgattttg cgcacccatc tgtcataaat atagaggata ccgtccagat
    24541 tgcaatctcc actgggggcc gcagccccgc aatggcaaga aagctaaagc taagagcaga
    24601 aaaactattc cggcagataa tcgacaaaga agacatttac cagatcaagc tgcaaaaaat
    24661 agcgcgcgat gccgcaaagt cgcagatccc aaccgtactt gggagaaaaa aattccttta
    24721 ctccgtttta aataacaaaa agataaaaca gttattaaaa gaagataact tgccaagtgc
    24781 acaaagggag gttatgaaaa ttctaggtga gtggaaatga gtgacataat aaatgcgcgc
    24841 gtaacgttta ggagatcgcc agtccacgtt ttagaaaaat tcacgttcaa ggacctagag
    24901 tccgcgtacg agtcattcag aaagcattcc ggcctcagag agtttgtaat tttgcagaca
    24961 tgcaacagag tagagatttt tggctcaact gatgagctta atttggaaaa gatcaaaaaa
    25021 acttgggcat cgctttccgg tcttgagcag agtgccttta aggaaaattt cgagacctca
    25081 gaagacgttg atgtctttga gcatctctta aaactaactg ccggacttga ttccatggta
    25141 gttggcgaag aacaaatcct aggccagata aaggaggcaa tcacaactgc acggcagctc
    25201 aaggcatcag gtgagcacat caacacatta tttgataagg caataaaaat tggaactcgc
    25261 gtaagaaact caactggaat caacaaaggg acaatatcag ttggctcgat ggcagtcaag
    25321 cttgcagaag aaaacattga tgatctaaa
//
LOCUS       DASWKH010000078        28485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_585505, whole genome shotgun sequence.
ACCESSION   DASWKH010000078 DASWKH010000000
VERSION     DASWKH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 28485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28485
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585505"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VGA92_05535"
     CDS             <1..949
                     /locus_tag="VGA92_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845910.1"
                     /translation="FNYKKTEPITDETFLSKNVKEWQEVSFDELMNYHENYGKRGFFG
                     DLGALLVKNEMLLQLERENIINANDDFDVYQGMTLTSLPPHVSFEAVVNGTDGNSYRL
                     QGGTFANSVGNVKITKLAFYDTLQKLSVDFILSQNQTIRIEEDANGARAIPYDMIIRA
                     DKDNTVEFENTLIIPIRIQGDGDWKNPNWYGPTTLPYGKATMTFNTTGVIDWHARSLP
                     LPGSIATDHKGGGQISILSDKTSQLPLDVKGKIANAIIRNSEIPWSGIGTRTDGLYID
                     FNRAIFEMLPDANEYYTARAEQIVPFDMPIIIEEPVGMN"
     gene            952..1905
                     /locus_tag="VGA92_05540"
     CDS             952..1905
                     /locus_tag="VGA92_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845911.1"
                     /translation="MKTKLLIITGTVVTIAAVSIFLAFYQEIQLATDLVEKEDGPQSS
                     TVSNQDCAKLFDKIFDTWEEHVEKNYGGPGQPVMEPPTVEGLISGWWGGEEFRKTDCR
                     YRVNDWAYLVRHQDQVWGNVDWPQLEPFSQKEPTPLEKLLIQNQIDYRPDMVIVSGGL
                     AFGGDPGCGVVMDVDNRTHWFAINSISQPTEITLYSENPNQCKVNTSSCVCNVQMELT
                     ALTLKELSYFTPEEEEKYANILIDYLVKENINRTPKFMVGKFNINYTDPSAIGYCGEL
                     WGYNTIDYFDGAIVNDQVEDYGLAKELSPLCAIAEDAKWWE"
     gene            2129..2602
                     /locus_tag="VGA92_05545"
     CDS             2129..2602
                     /locus_tag="VGA92_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845912.1"
                     /translation="MSNIATILGIFAFFGAFAVFSNFAFAMCAVNEDWPDAPCFDVMP
                     VNREEYRAAWAPYYDYKGSEWMEQKKAEMLDARDNKRPSEWINGSSHYNVYSYYLSRG
                     EIAPVSPYDYPFFEDDFRYYLQFFAPGQGWLFFVMVGVSAGLIAGAAAFVFRRRK"
     gene            2604..3083
                     /locus_tag="VGA92_05550"
     CDS             2604..3083
                     /locus_tag="VGA92_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845913.1"
                     /translation="MTQKNEKIFLAVISGYFFAIFSIVVFGIVIATQTSDSMPVEVVT
                     VHGNKTTKDSVVITDAPPLSTSFAAVFSNFSFVFPFILAGVGVWLPWISIMSGQLPTP
                     HETSVLVLSFWILSMVSIPITIKLSKGIPMWRRIPYIYLVAVLIGGTAIWLWTGFGN"
     gene            3096..3404
                     /locus_tag="VGA92_05555"
     CDS             3096..3404
                     /locus_tag="VGA92_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845914.1"
                     /translation="MKTLLLAIIISMVVFIGIQTIVPFPYGLIFGIAIPAIIIWRTIK
                     ITDVNQHSLLSYRRDDPKSDKEKEQNKEAYRILKKRFLEGEITQEEFDRLREPFGDME
                     "
     gene            3406..4044
                     /locus_tag="VGA92_05560"
     CDS             3406..4044
                     /locus_tag="VGA92_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845915.1"
                     /translation="MDKKKMKFELYLIAVVGLLVVGILYLIYLNPDDVIYYNSLQKIQ
                     FEKYGDYHFELVDGSEGHWATISSIKLIDNDTIEVNFNDNDYKINTKEGVVIYAVPKF
                     EYTTTVTKGKTFISTCHGESAPSFLNYMGIVQIEGKYYYQFYHNSGVLLPDGMTCKFP
                     EIIKHSLDANLDLSGYFVPIPPVQTSGKYYKNPDVIVDVPYPCDVLECENAN"
     gene            4034..4210
                     /locus_tag="VGA92_05565"
     CDS             4034..4210
                     /locus_tag="VGA92_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845916.1"
                     /translation="MQTNYKIMIAGVLLFILGMTLMLNKDYRHFLFDIHPDLEIVGFL
                     VPPSAGLVITQGLE"
     gene            4248..4742
                     /locus_tag="VGA92_05570"
     CDS             4248..4742
                     /locus_tag="VGA92_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845917.1"
                     /translation="MKTRLLVILFVPSILFPASNVFAPPSPNEWPAAPYCPGGCSIDY
                     LKEKWSEYYDYKGSEWMEARKQEMLNAMSNGTLNEWLNADPMNAHYNVHAYYFYKGEI
                     PNHEGKFIDQVAQEIFLSDMENSLQNNRFPLGEHMYVNFTFLLTVIGGSIVGIIFAIR
                     RKRK"
     gene            4739..5056
                     /locus_tag="VGA92_05575"
     CDS             4739..5056
                     /locus_tag="VGA92_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845918.1"
                     /translation="MKTNYKINPFFGLNIGIVAFLSLGAAYFTIMPMIMWNVSFDRMV
                     SGATQNYAMIGLWLIYFTNIAFLPITMKKIDRTWRATYVVFLIGVVVFIIFILNPFPY
                     EFE"
     gene            5299..6117
                     /locus_tag="VGA92_05580"
     CDS             5299..6117
                     /locus_tag="VGA92_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845919.1"
                     /translation="MRLNLSTGPLSHEEQKIYLYLESIGKNAFKVSELDIKKLGIKQN
                     YLYVLIGRLEKKGWITGVGKGVYLRLPAATAIDGKVYLEDPFEVAMKMFKGYLAFQSA
                     LKVHGLSEYEPFTIYMATKRKSETIPLLKHYEIKAIKFGKRFTGFEKMGRYAVSTKAK
                     TFFDCFYHPKYAGGYAEVLKSLYNAGHIDWKEMEKYLERFGSSSICQKIGYLISLLKE
                     TEFEYPLDFIDYLKGRIGNKTKLDFGQGGGSYNKEWMIVDNIGAKKLLSWWYHG"
     gene            6110..6928
                     /locus_tag="VGA92_05585"
     CDS             6110..6928
                     /locus_tag="VGA92_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX9845920.1"
                     /translation="MASLSIREINQAAARTGIGRQYILKEVRVFDIWSKICPALLSKE
                     ITAHAKVICKGGTSLNKVFLGRVQRFSEDLDMDIFFKKRLEREEKIQFIKNKIIPLVS
                     SSYTVPKEARKRNIILFFCKFKNELGMDDNVQLEFNIGESRTGVDEIATARSSILQLK
                     LDNIPVYSFDTLVAMKMKAFYEREEGKDVYDLYYSLRSKKNNWSKVISILKDVLRSSG
                     IDYKVFKKELPNKLSDSKKMRSLHASTNPYIPKNLRIDFLKASELISKKAVPLL"
     gene            7390..7611
                     /locus_tag="VGA92_05590"
     CDS             7390..7611
                     /locus_tag="VGA92_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9845921.1"
                     /translation="MQHSTSAVSKKTYQVVLDRDEDGMIFARCDELHANAEGKTEAEA
                     KINIKESIELMVEHLNKDKSFSLKFIRKY"
     gene            7611..7856
                     /locus_tag="VGA92_05595"
     CDS             7611..7856
                     /locus_tag="VGA92_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9845922.1"
                     /translation="MSKIPRVSGNQLIKYLVNKKGFRVSHRKGSHVSLHNENKHKFTS
                     VPSKNDDLGTGLMLQILDDCGITREEFISDYQGGLLK"
     gene            8135..9022
                     /locus_tag="VGA92_05600"
     CDS             8135..9022
                     /locus_tag="VGA92_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9845923.1"
                     /translation="MVTIKEKILKGQTYYYLEHSVRENGKVRPKIKYLGRKIPKNIEE
                     IKKNFLNDISGARWHADVDRIRKNFSKNQRLMPKSVKEKEIENFAINFTYDTQRIEGS
                     TLTRKETADLLERGITPKNKPMRDAQEAEAHRDLFFEILKSKKDLSLQEVTDWHWKLF
                     NKTKADLAGEIRKYQVAIKGSKFMPPTPVDVYPMLTEFFEWYNKNKNKLHPVELAAIA
                     HLKFVTIHPFGDGNGRISRLIMNFILDKKKYPMLDIPYEGRNSYYNALERSQITKKER
                     VFLQWLVKRYVKGYRRYLK"
     gene            9543..10352
                     /locus_tag="VGA92_05605"
     CDS             9543..10352
                     /locus_tag="VGA92_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845924.1"
                     /translation="MPFLIYFGGIIEKILKEKTYSIKDSIILTAASFIVIRFVDFATT
                     SLEGIDRTVPYTTMGIGFIGIIMPVAMFLGFIMRNRVRNKKTLSVKDLSKIIHSLSAK
                     DFLWIISSGVTIIILGYLLGKVSFDDKADVPRVLADFGYVYVIMPLSIYLRDTIHKIR
                     NKKTIGVKNIIILFAAGLASVGFIGLNINMMPNQAFSIDTEPHVGDKVLHVALYFLGL
                     SCIIISLAKVSSMIITTFKNKNRIKIKNYPGAIMEYVHKNDVVNEIKKIWK"
     gene            complement(10425..11495)
                     /locus_tag="VGA92_05610"
     CDS             complement(10425..11495)
                     /locus_tag="VGA92_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845925.1"
                     /translation="MKIIIAVFFVFVLVLTSIQPSFGRTVTYETLTPLKWGQPNVCIF
                     EPDYVSDTMIFGPITKSMVSETKSALDQWESLLKERERSRDDMSKWEINYSIVPLHQQ
                     RSFDKAECSVLIQFLPVPNTNEAWHRALGAAENKDGSDNDFRLISVFYQKMDYCEDHR
                     DEQYIYYKPCYINDVILSVQIGNTVRHEFGHALGLGHYYLDDIPDAYWNEFVVPYPSI
                     MVMVGADIYEQQQIKQIDIDKVREIYGENGFRKPDLGKNSAAKSSESVSIIPDWVRSN
                     ARWYSEGMSNESDLTEGIEYMIEHGIMKIPNLTKQAHEVSESKIPDWIKSNAKWWSEG
                     SISDSDFIKGIQYLVEKGIIRV"
     gene            complement(12105..14306)
                     /locus_tag="VGA92_05615"
     CDS             complement(12105..14306)
                     /locus_tag="VGA92_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX9845926.1"
                     /translation="MNQNSISLKVLEAYSRDVGNGTARLDYEATDSLGAATGDVLEIK
                     GKKRTVAKCLPLYPSDEGKGIIRVDGVTRNNLGIAIGDKVEVRKITAIPAEKIIVAPL
                     EAAPQIDERYLAESLENTPLTKGDYVTVRYFGGKLTFLVLETTPSADAVIVTQKTAFF
                     VGEKNESLHGIPQISYEDIGGLTDEIRKVREMIELPLRHPEIFEKLGVEAPKGVLLFG
                     VPGTGKTLLAKAVANESNVHFISISGPEIIGKFYGESEARLREIFKEARERSPSIIFI
                     DEIDSIAPKREEVSGEVERRVVSQLLSLMDGLAARGKVIVIAATNRPSSLDPALRRPG
                     RFDREIEIKVPQKKGRHEILSIHTRNMPLSDDVDLEKFASISHGYVGADLEYLCKEAA
                     MKCLRRILPEINLADEKVSVEILDKLVVNTEDYTNAFREITPAGMREVYIENPDVSWD
                     QIGGLEAVKRELQEAVEWPMKYPTFYNNIGYKNPRGILLYGPSGTGKTLLAKAVATES
                     EANFISVRGPELLSKWVGESEKGIREIFRRARQASPCVIFFDEIDSIASVRGLGGDSS
                     ATERVVSQLLTEIDGIESLHGVVVLAATNRADMIDPALLRPGRFDKAIQIPMPDNEGR
                     KKILEISTIGIPLDKEASSGTNENPDYVNLEKIANASEGLNGADVAAIANTATSLVIH
                     EFLAKYPDQKEAEKQVGGTKVTMRYFEDALKKVKLQKEIKIAEKIDVQYYR"
     gene            complement(14401..14886)
                     /locus_tag="VGA92_05620"
     CDS             complement(14401..14886)
                     /locus_tag="VGA92_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845927.1"
                     /translation="MQISDAESIESNKSEWEGIAENDDKNQIQPTNEYHSVCDIWRDS
                     IHKLITKVEYQTPIYLQAYSQVHTEFLHCVDNLFGTCYIWQKQYFDRLGIDQKIVNSY
                     GRLCESGVNSTIDLLDIYASYKKFQADMAVDAMKIGNNYLHWWIDMFGKTVSFWSPHF
                     R"
     gene            15015..15449
                     /locus_tag="VGA92_05625"
     CDS             15015..15449
                     /locus_tag="VGA92_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845928.1"
                     /translation="MSKCSVLGPLSFEQSKLVSKFCKDVLLLDDQVVFVSVISRHGRQ
                     IDSVARGDSSFNDLTKHELEQVYLQRMLQTQMIKDFDDKLSKLSVAILERESCIEGIF
                     PFYDGVLLVVFNSHPQILDSVKKTSKSINEFNFEIAKSGVTC"
     gene            16045..17148
                     /locus_tag="VGA92_05630"
     CDS             16045..17148
                     /locus_tag="VGA92_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9845929.1"
                     /translation="MVESNYDILGVNTGASQKEIRDAFRRLVLDHHSDRGGEDEEKVK
                     KIIQAYEDLKVGKTYPDSEDERLKKARVYTGDSEEERKKRNLTLSGDVAREMKLAEDW
                     AELLNRNHATGSRLFGSKELGEVEFERKETGELLIKGKFWAGHLTYGGPIIMAGSITS
                     PYFAQQEETKTTITVRNGNFMMLDPLQNNFTIEHGAKIIAENGDIIVGNLLGIKEILQ
                     DPQGRVGIYITKEHFTELRAPKGKVVAGTARETVFLDADTVVVNNLINNIKVRARHIT
                     IFGSTVNYNCELELRAGGSLVFHDEGSGFALSDDALVKLENGKWFKLQELKTSNMVGH
                     GRQITYEYLDNLGKKSEKSQGAKINLGKFFKKK"
     gene            17154..17948
                     /locus_tag="VGA92_05635"
     CDS             17154..17948
                     /locus_tag="VGA92_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX9845930.1"
                     /translation="MELSIGCTGWGYDGWVGPFYPKSLPNSKFLNHYSEIFDITEVNS
                     TFYAIPQLQIVKKWYSDTPPQFKFTAKMPKIITHENRLQNLAPYLEQFLESMKPLGSK
                     FLLSVVQLPPPLSFEDAKPNLEHLESFFKNSSYVIEGRHQSWFSENALRYLTDKKICL
                     VWSDVQGIKNTAPLTSDLVYLRVIGDRSIPEKEFGKVLQDRTTDLKGWAEKINHLKDK
                     ITLAVIMANNRYEGFGPATANKLRLLLGLDAIAFSDTRQKTLENYK"
     gene            18344..18958
                     /locus_tag="VGA92_05640"
     CDS             18344..18958
                     /locus_tag="VGA92_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845931.1"
                     /translation="MVSRAEIYQMAKTGCVAGLVGGFALFSSFFWIDSEVGVPFGTFY
                     KAVGMVVGLDGLAAIAFGFFAHMLTAALIGAIFCICSTFHKLLHISSVPKGIIAGAVT
                     GIQVYAIFFMPITVYVMFPMVSAQASGLSGSVTQEDMQIAKMLVDTSDAILWGSLVLH
                     VLYGAVMGLFSSMMMYEDYHMKEKSKKEKKENWKKFESENWPST"
     gene            18955..19503
                     /locus_tag="VGA92_05645"
     CDS             18955..19503
                     /locus_tag="VGA92_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845932.1"
                     /translation="MTTTVSQLSFTKRVQIGCVAGLVGGFAIFVSIFVIDLSLGASQG
                     TFYKVVGLPAGIAGIYATLFGMVLHMLTAALMGTIFGIGSGIHKLLEISSLKKGAVAG
                     IVTSLVVFLVFFMPISTFLIIPLLQSSAIELGEASQLLVSMELIMIGSLELHVVFGMV
                     MGIFFAVAVQHESKKLVEATAV"
     gene            19504..20124
                     /locus_tag="VGA92_05650"
     CDS             19504..20124
                     /locus_tag="VGA92_05650"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX9845933.1"
                     /translation="MLRIVAISVIIGTFLIGTYYVVEASYIQPIQSDRIAGALGEVQV
                     GLTYEKFVSLSEGERSVLVQQMPSHTITMLLQEARNHPSFIAEKTDVIKEQSGLSEIR
                     LVKLSQISGLKGYDSKGEAAIIISGDKAFLRLQEFGVTSGIDQHLYLTKDGTIATGID
                     LGRLKASQGDQYYDITGIDTESHNVLIIYSKAFDTYYAHAKFLKTQ"
     gene            complement(20303..20476)
                     /locus_tag="VGA92_05655"
     CDS             complement(20303..20476)
                     /locus_tag="VGA92_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845934.1"
                     /translation="MVGPKDGGFGFDQSKKYSSVDNLDEICVQCQAGQHAKCLAKSKQ
                     IPNCECEHCIIFG"
     gene            complement(20520..20663)
                     /locus_tag="VGA92_05660"
     CDS             complement(20520..20663)
                     /locus_tag="VGA92_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845935.1"
                     /translation="MANLGSIIKVFSFVVVGIVTIIVGAFMLRGTMNLWDKQKDSGDE
                     DKS"
     gene            complement(20665..21411)
                     /locus_tag="VGA92_05665"
     CDS             complement(20665..21411)
                     /locus_tag="VGA92_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX9845936.1"
                     /translation="MSDRVFVMLFAFMIVFVMLPSFASQDLFIPKWFLVVYQYFKDEQ
                     ISAKEFESAITYLQKVGVMRLSTDSQDESITNFLITHSIIEQNSAGHSEFSNCISHWY
                     ITGYFTPAESDYAGKVITVNVDGASYQFREDFVTEIKTEGWGKTLSGKYLGWFDESFH
                     LSDFPLDATGNNLESHMIAVDPSIIPQNSRVMIPTLLSPWNEVVFLASDIGTSIIGKH
                     IDVYTGEGKDAHEETYRITGHENIVCLEVK"
     gene            21526..22083
                     /locus_tag="VGA92_05670"
     CDS             21526..22083
                     /locus_tag="VGA92_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger protein"
                     /protein_id="HEX9845937.1"
                     /translation="MKLSLSQPRTKANSCLFCGEILKTPSKCAYCGFKFCQDHLLTDD
                     HQCGKTRYAEYVRKTNDDSVPNLASGMFVVVCDTCGYRSTKATLIEFAGEELIQHMQV
                     IGCSENTFLEEIGDATPFDSQSVEIKSNDQDLVTRVGSTSQNNSEPKDGIVEQLAKLA
                     SLKEKGVLSNEEFLFIKKEIIKKLK"
     gene            complement(22096..22872)
                     /locus_tag="VGA92_05675"
     CDS             complement(22096..22872)
                     /locus_tag="VGA92_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845938.1"
                     /translation="MGFFRRAKKEEEPIGQAKEAEPVQIKSPEEIQKEQITSELEYLQ
                     GELGSKTDHLNSISEKLAKVKEEYDHVVGELMSAKRELNEKKSEIESIIGQYDEKKTL
                     LSELEGANKELVAIKSEYVKYKAESEELKNKIKTLQSSYEENKRAHESAGQKITKEKA
                     ELESARKEIEEIRREYESKKKEIELAKKELKFIEEQMSNVGDRATPKNIVAAASSVVA
                     SLNTKLLAAQKELETLKIALQRERADHAETKKKLDDLSQS"
     gene            complement(22910..23095)
                     /locus_tag="VGA92_05680"
     CDS             complement(22910..23095)
                     /locus_tag="VGA92_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845939.1"
                     /translation="MAHSEHNRPKKSGAFLLIILGALLFIITPAWFSESPEFGLALIV
                     LGFLIGGLGFYLRFIRK"
     gene            complement(23097..24692)
                     /gene="lysS"
                     /locus_tag="VGA92_05685"
     CDS             complement(23097..24692)
                     /gene="lysS"
                     /locus_tag="VGA92_05685"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9845940.1"
                     /translation="MSEADQKIIGKGTWIDKLAYEMIEREKQIGRDTSLLRVESGLGA
                     SGIPHIGSLGDAVRAYGVKLALEESGFQSELIAYSDDLDGLRKIPEGLPEWLGEHIAK
                     PVSLIPDPFGSCHNSYGEHMSGLLLDGLDKLGIKYKFQRAFDTYRQGLLKNQIHTILT
                     NTEKIGNKIAEMVGQEKYQKFLPYYPVCAKCNRLYTAQAYEYDAENMKVKYRCSNAEI
                     GSQILQGCSHEGEADISKDLGKLAWKVEFAARWHAFDIRFEAYGKDIMDSVKVNDWVS
                     DEILGHPHPHHVKYEMFLDKDGKKISKSAGNVVTSQKWFRYGTPKSMLLLLYKRITGA
                     RELGFEDIPTLMDEYNDLEDIYFGKIKLDNEAKTVKSKGLYEYTNLLNPPKTPPVHVS
                     YRLLIELCRIFKEDRINLVMKKLIEYRAIKNPEPHIADLITLAGNYADDFEGVELIQM
                     TIDNQMKKALNQLVEILYANDDPQDLQNKIYNVAKENNIEPREFFKVLYRIILSANQG
                     PKIGPFIIDIGRNKVAELISEYL"
     gene            complement(24702..26282)
                     /locus_tag="VGA92_05690"
     CDS             complement(24702..26282)
                     /locus_tag="VGA92_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HEX9845941.1"
                     /translation="MQQTSTIFAKACVEISRDLLTIDSPTKNQIKAQIKKICTKYALE
                     RIPRNYEILSTVSGGDYTRLQKILLKKPIKTASGVAVIALMPMPYACPHGRCTYCPGG
                     IEFNSPNSYTGNEPATINAIESQYDPKKQILSKIEKLVAYGHDPTKMELVIVGGTFLF
                     MPKDYQQNFIKSCYDALNGFDSQDLEESKTNNEKAHIRSVGFTIETKPDYCKQEHVDL
                     MLQYGVTRVEIGVQTLQENVYKIVNRGHTYSDVVESFQISKDAGYKIVAHMMPGLPTM
                     SPDGDIADFKKLFEDDDLKPDMLKIYPSLVLQHTPLYQQYQQGEYLPYSDEDMIRVLT
                     EVKKNIPRWVRIMRVQREISPQEIIAGPKSGNLRQIVHSNLKKQNLSCKCIRCREIGL
                     TNEPQKEIFLHRNEYTSSGGDEVFLSYDDSDDKIYGFLRLRRPSQMAHRKEVTGNSCI
                     VRELHIYGRSLKIGEREDGQIQHSGLGKNLMSEAEKIAKEEFDAEKLLVISAVGTRQY
                     YHKLGYLLDGPYMAKLLV"
     gene            complement(26328..26684)
                     /locus_tag="VGA92_05695"
     CDS             complement(26328..26684)
                     /locus_tag="VGA92_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845942.1"
                     /translation="MVEDKNFVDMCPVCKQGVSKADMDFQKGKVFHVQCFKEHGGSFP
                     TPNSELAYLSAKTRIELVQTKNMMARAGLEEPKTAKTLTNKARKKHTKKNKRKKAKTG
                     KIKAKKAKSKKAKKRR"
     gene            complement(26741..27958)
                     /locus_tag="VGA92_05700"
     CDS             complement(26741..27958)
                     /locus_tag="VGA92_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9845943.1"
                     /translation="MEAHFVQVVIGVCILLFAAKLMAELFVRLKLPIVLGELLAGMII
                     GPFAIGSLIVIEGETLLQLSTEVKILGEIGAIVILFLAGLEMTPREFIKGGKASFTVG
                     TLGVVVPFVVGFFIFQLFGFHAYQAMLVATALTATSIAISVQVLSEYGKLKSPEARLI
                     IGAAVVDDILAIAVLSVVTSLGVSGESIDVVDVSFTIIKVLAFFAAMAVAAIFLVPKL
                     VNSKLWKAGGSIEGVVTAAFFGAAGVAAVIGLSPILGAFVVGMALSSTKLFERVEHYA
                     SKLGLIFAPLFFAIIGAQVDFRGIGANPEILLIGAVVIGVAVATKMFGCGLPAWLFLR
                     NGPQAKRVGIGMVSRGEVGLIVAGVGLSSGVLTSNVYTTIVLMVAVTSIITPIWLKME
                     YRKELHRNPESQN"
     gene            complement(28027..>28485)
                     /locus_tag="VGA92_05705"
     CDS             complement(28027..>28485)
                     /locus_tag="VGA92_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX9845944.1"
                     /translation="HSNGYSLARKVLLKKYSISDKVSGVGKLGDALLTPTEIYVKPIM
                     EIVLRCKVHGLANITGGAFTKLLRLKKTGFVLDSMPEPPKIMQLIEDLGVTQSEMYKT
                     FNMGIGFCVVAPKQETQRIISICKKYKMKSQDIGFISDRKGVYVNSKKLA"
BASE COUNT         8333 a         5771 c         5822 g         8559 t
ORIGIN      
        1 ttttaattac aaaaaaacag aaccgataac tgatgaaacg tttctctcaa agaatgtaaa
       61 ggaatggcaa gaggtgagtt ttgatgaatt gatgaattat catgaaaact atggaaagcg
      121 cgggtttttt ggtgatctgg gcgcattgct tgtcaaaaac gagatgcttc tgcaactaga
      181 acgagaaaac atcataaatg caaatgacga ctttgatgtt taccagggaa tgacgcttac
      241 gtctttacct ccacatgtct cttttgaggc agttgtaaac gggactgatg gaaattcata
      301 ccgtttgcag ggtggaacgt ttgccaattc ggtaggtaat gtaaaaatta caaaacttgc
      361 attctatgac actttacaaa aactgtccgt tgatttcata ttgagtcaaa accagacaat
      421 aagaatagaa gaagatgcca atggtgccag agcaatacca tatgacatga tcatccgtgc
      481 cgacaaggac aacacagtcg agtttgaaaa tactttgatc ataccaatca gaattcaagg
      541 agacggtgac tggaagaatc ctaattggta tggccctaca acccttccat acggaaaagc
      601 caccatgacg tttaatacta caggtgttat tgactggcac gcccgttcct taccgcttcc
      661 gggtagcata gctacagatc acaaaggagg gggacaaata agcatactat ctgataaaac
      721 aagccagctg ccattggatg tgaagggcaa gatcgcaaac gcaataataa gaaattctga
      781 aattccttgg agcggaattg gtaccagaac agatgggctt tacattgatt ttaaccgcgc
      841 aatctttgag atgctgcctg atgccaacga gtactatact gcaagggcag aacagatagt
      901 tccatttgat atgccgataa taattgaaga accagttggg atgaattgaa aatgaaaacc
      961 aagcttttga taattactgg aacggttgta acgattgcgg ccgtttccat ttttcttgca
     1021 ttttatcagg aaattcaact agccacagac cttgttgaaa aagaagacgg ccctcaatcc
     1081 agcaccgtct ctaatcaaga ctgtgccaag ctctttgata agatatttga tacttgggag
     1141 gagcatgtgg aaaaaaatta tggtggtccg ggccagcccg tgatggagcc tccaacagta
     1201 gaaggattga tttcaggctg gtggggcggg gaagaattca gaaaaaccga ttgcaggtat
     1261 agagtaaacg attgggcata tctggtaaga catcaggatc aggtatgggg taatgttgat
     1321 tggccacaac tagaaccatt ttctcagaaa gaaccaacac ctcttgaaaa actgctaatc
     1381 caaaatcaaa tagactatcg tcccgacatg gtgattgtat ctggtggttt ggcctttggg
     1441 ggtgatcctg gctgcggtgt cgtaatggat gtggacaatc ggacccattg gtttgcaata
     1501 aactctattt cacagcctac agaaattact ctttattcgg aaaatcctaa tcaatgcaaa
     1561 gtcaatacaa gttcgtgcgt ttgcaatgta caaatggagc tgaccgcact gacactaaaa
     1621 gaattgtcgt attttactcc agaggaggaa gaaaaatatg caaacatact gatagattat
     1681 ttggtaaaag aaaacatcaa cagaactccc aaattcatgg ttggcaaatt taacataaac
     1741 tacaccgatc cgtctgcgat agggtactgt ggagaattat ggggatataa cacaattgac
     1801 tattttgatg gggcaatagt aaatgatcaa gtcgaggatt atggattagc gaaagagctt
     1861 tcacctttat gcgcaatagc cgaagacgca aaatggtggg aatgaaaact agctccagaa
     1921 cattattaaa aataacagca cttgttttac cagtaggctc atctgttccc attgcaatcg
     1981 gtttgcaata catactacct tatccgtatt gatacataac ctcattcgtt tccgaaatgg
     2041 gtcttgtcta tgtcgcaata cgaatgtgga tttattctga taaaaaacag gagtaacact
     2101 taaggaagat acgaaaaaag ctgccttcat gagcaatatt gccaccatac ttggaatttt
     2161 tgcgtttttt ggagcatttg ccgtgttttc taattttgct tttgccatgt gtgcggtaaa
     2221 tgaagactgg cctgatgctc catgctttga tgtgatgcca gtaaacagag aagaatacag
     2281 agcggcttgg gcgccatact atgactacaa gggctccgaa tggatggagc aaaaaaaagc
     2341 agagatgctg gatgcaaggg ataacaaaag gccctcagaa tggataaacg gcagctcaca
     2401 ctacaacgtg tattcgtatt atctgtccag aggggagata gcccccgttt caccgtatga
     2461 ctatccgttc tttgaggatg actttaggta ttatctgcag ttttttgccc ctggtcaggg
     2521 gtggctgttt tttgttatgg ttggtgtatc tgcaggcctg atagctggag ctgccgcgtt
     2581 tgtatttagg agaagaaaat aaaatgacac aaaaaaatga aaaaatcttt cttgcagtta
     2641 tatctggata cttttttgca atattctcaa tagttgtatt tggcattgtt attgcaacac
     2701 agacatccga ttcgatgcca gttgaggtag taaccgttca tggaaataag actacaaaag
     2761 acagcgtggt gattacggat gctccccctt tatccacatc gtttgctgcg gtgtttagca
     2821 acttttcatt tgtatttcca ttcatcttag caggcgtagg agtgtggctg ccttggattt
     2881 caataatgtc tggtcagctt cccaccccac acgaaacaag tgttctggtg ttgagtttct
     2941 ggattttgtc catggtgtca attccaatca caatcaaact ctcaaaagga attcccatgt
     3001 ggagacgaat accctacatt taccttgttg ccgtcctaat agggggaacc gctatttggt
     3061 tgtggactgg gtttggaaat taggagaaaa agaaaatgaa aactctgcta cttgcaatca
     3121 taatttcaat ggtagttttt atcggcatac agacgatagt gccatttcca tacggattga
     3181 tttttgggat tgcaattcct gcgatcatta tttggcgaac cattaaaatt actgatgtaa
     3241 atcaacactc acttttaagc tacagaagag atgatccaaa aagtgacaag gaaaaagaac
     3301 aaaacaaaga agcatacaga attttgaaga aaaggtttct ggaaggcgaa atcactcaag
     3361 aggaatttga caggctccga gagccgtttg gtgatatgga gtaaaatgga caaaaagaaa
     3421 atgaaatttg agttgtacct tattgccgtt gtagggttgc tagtagtagg aatattgtat
     3481 ttgatttatc tgaatcctga tgatgttatt tattacaatt cattacaaaa gatacaattt
     3541 gaaaaatatg gagactatca ttttgagcta gtcgatggca gtgagggtca ttgggccaca
     3601 atcagcagta tcaaattaat tgataatgac acaattgagg ttaatttcaa cgataacgat
     3661 tacaaaatta acacaaaaga aggcgttgtt atttacgctg tgcccaagtt tgaatacacg
     3721 actaccgtca caaaggggaa gaccttcata tccacatgcc acggggaatc agcgcctagc
     3781 tttttgaact acatgggaat agtccaaatc gagggaaaat attactatca attttaccat
     3841 aattcgggcg ttcttttacc ggatgggatg acctgcaagt tccctgaaat cataaagcat
     3901 agtcttgatg caaatttgga tctaagcggt tattttgttc caattccgcc ggttcaaacc
     3961 tctggaaaat attacaagaa tcccgacgta attgtggacg ttccataccc gtgtgatgtt
     4021 ttggagtgtg aaaatgcaaa ctaattacaa aataatgatt gcaggagtat tgctctttat
     4081 tttaggaatg actttaatgc tcaacaagga ttacaggcat tttctttttg atattcatcc
     4141 ggatctggaa attgtgggct ttttggttcc tccatctgct ggcttggtaa tcacacaggg
     4201 attggaataa tgctggagtt tgcaagaaga aaatatgggg tacaaaaatg aaaaccagac
     4261 tgttggtcat tttattcgta ccttcaatat tgtttcctgc gtctaacgtt tttgcacctc
     4321 catcaccaaa tgagtggcca gcagcaccgt attgtcctgg aggctgttct atagactacc
     4381 taaaagaaaa atggtcagaa tattatgatt acaaaggttc tgagtggatg gaagccagaa
     4441 aacaagaaat gctcaatgcc atgagcaatg gaaccttaaa tgaatggtta aatgcggatc
     4501 ctatgaatgc gcactataat gtgcatgcgt attatttcta caaaggagaa atacctaacc
     4561 atgagggcaa gttcatagat caagtagctc aagaaatttt tttgtccgat atggaaaaca
     4621 gtctacaaaa caaccggttt ccgctaggcg aacacatgta cgttaatttc acatttcttt
     4681 tgacagttat tggcggatcc attgtaggaa ttatttttgc aattaggaga aaaagaaagt
     4741 gaaaactaat tacaaaataa atccattttt tggactaaat attggaattg ttgcttttct
     4801 aagcttggga gcagcctatt ttacaatcat gccgatgatc atgtggaatg tcagttttga
     4861 tagaatggtc tctggggcaa ctcaaaatta cgcaatgatc ggattatggc tgatttattt
     4921 tacaaacatt gcttttttac caataactat gaaaaaaata gacagaacat ggagagccac
     4981 ctatgttgtt tttttaatcg gcgttgtagt tttcatcata ttcatcttaa atcctttccc
     5041 ttatgaattc gaatgatttc ttttactcct gcaaaaaata agaagtggta ctgtcagccc
     5101 cacaatcgct tcctgccgtt attcaaggtg ctcgctacaa ctgttctaat agtactattt
     5161 ttgcaatgtt tagtttaagg catgtcagta attatgggtg agaaaaagca ctaaaatctt
     5221 actatggtag ttagaaatgg ttcttaaaac tccgttcagt gagaaaagga tttaaaatct
     5281 taccaccata gtaagattat gcgtctaaat ctctcaacgg ggcccctcag ccatgaagag
     5341 cagaagatct acctctatct ggagagcata ggaaagaacg ccttcaaagt ctctgagctt
     5401 gatatcaaga aactaggcat taaacaaaac tatctgtacg ttttgattgg gaggttggaa
     5461 aagaagggct ggataacagg agttggaaag ggggtgtacc tccgcctgcc ggcagctacg
     5521 gccattgatg gtaaggtgta cctggaagac ccatttgagg tagctatgaa gatgttcaaa
     5581 gggtatctcg catttcagag cgccctcaag gtccacgggt tgagcgaata tgaaccgttt
     5641 acgatctata tggctactaa gcgaaagtct gaaacaattc cgttgctaaa acattacgaa
     5701 atcaaagcca taaaattcgg caagcggttt acaggattcg aaaagatggg caggtatgcc
     5761 gtgtcgacaa aagcaaagac gttctttgat tgtttctatc atcccaaata tgctggaggt
     5821 tacgcggagg ttctcaaatc actctataat gcaggccata ttgactggaa ggagatggag
     5881 aaatatcttg aaaggtttgg ctcttctagc atctgtcaga aaatcggtta tctaatttct
     5941 cttcttaaag aaacggaatt cgagtatcca cttgatttca tagattacct gaaaggaagg
     6001 ataggtaaca agacaaagct tgactttggc caaggcggtg gttcgtacaa caaggagtgg
     6061 atgatagtag acaacattgg ggcaaagaaa cttctttcgt ggtggtatca tggctagcct
     6121 tagtataagg gagatcaatc aggctgcagc aagaaccggc atcggacgcc agtacattct
     6181 caaagaggtg cgcgtctttg acatctggag caagatctgt ccggcactgc tctccaaaga
     6241 gattaccgcc catgccaaag tcatttgcaa gggaggaaca tccctcaaca aggtgttttt
     6301 gggaagggtt cagaggtttt cggaagactt ggacatggac atatttttca aaaagagact
     6361 cgaaagagaa gagaagatcc aatttatcaa aaacaaaata atcccacttg ttagcagctc
     6421 ctatactgta ccaaaagagg cacgaaaaag gaatatcatc ttgttcttct gcaagttcaa
     6481 aaacgagctg ggaatggacg acaatgttca gctcgagttt aacataggcg agagcagaac
     6541 aggagttgat gaaatcgcta ctgctagaag ctcaatactt cagctgaaac tggacaacat
     6601 accggtttat tcctttgata cgctggttgc aatgaagatg aaggcgtttt atgaaagaga
     6661 ggaaggaaag gacgtctacg acctgtatta cagcttgaga tccaagaaga acaactggtc
     6721 taaagtcatc tcaatattga aagacgtcct tagatcgtca ggaatagatt acaaagtgtt
     6781 caaaaaggag ctcccgaaca aactttcgga ttcgaaaaag atgaggagtc ttcatgcatc
     6841 tactaaccca tacattccaa agaatctgag aatcgacttt ctcaaagcat ctgagctcat
     6901 atctaagaag gctgtaccgc ttctttaact aaatttgcta caagaaaatt ctgaagcaga
     6961 tcatgagatg attaataatt cacagtttag attctaaaaa taaaaattga taggagttta
     7021 gcagccactc tgatggcttt ctgcctgttt tagatgttaa ggaattcaag accaacggca
     7081 tcctaacccc attcagttca tcatctacgc atcaatcttt tgtaatcaaa aaacacttaa
     7141 gcaatgtatt gtacgccaaa acaacaaagg aacccaacat gaaaaagcaa aaaaactctg
     7201 ggttccgcaa gctggagcac acggactccc ccgggcccgc cttcactgtg gacgcgagtc
     7261 tcattcaatc cactcatgtt caaatcctca gaactgggtt ccaatttcgt aatgaatact
     7321 tggtgccaaa tcactgtggg ctaattgtgt actattcttt ttatatgtcg tactgacatc
     7381 gtattataga tgcaacattc tacctcggct gtatcaaaaa agacatatca agtagtttta
     7441 gatagggatg aagacggtat gatctttgca agatgcgatg aactccatgc aaacgctgaa
     7501 ggcaagaccg aagcggaagc aaaaatcaac atcaaagaat caatcgagtt gatggtggag
     7561 catttgaaca aggacaaaag ttttagtctt aaattcatcc gcaagtatta atgagcaaga
     7621 ttcctcgagt ctcaggaaat cagttgataa aatatcttgt taacaaaaag ggatttcgag
     7681 tatctcacag aaaaggaagc catgtgtctc tacacaatga gaacaaacac aagttcacgt
     7741 cagtaccatc aaaaaatgac gatcttggaa ctggtctaat gttacaaatt ttggatgatt
     7801 gtggaataac tagagaagaa tttatttccg attaccaagg cgggctttta aaatgaatgt
     7861 aacaaacgcg tatcatgatt tcaatttaat cgaaaaacca ctatggtacg agtaaatttt
     7921 tacttgcatc cccccgggcc caccaaaaac ttttttgaaa gctagaagaa agatgtagaa
     7981 taattctacg caatttttta aatgttatcc gtatctcatt tgtctggtgt gaaaccctat
     8041 agtggcggtt gttcgctagg cgttgtttat caggttaccc ttacattttg agtaaaagtg
     8101 ataattatat aaatatgtaa gggtaactaa ttgtatggtt acaataaagg agaagatcct
     8161 gaaaggacag acctattact atctggaaca cagcgtcaga gaaaacggaa aggtgcgccc
     8221 aaagatcaag tatttgggga gaaagatccc caagaacatc gaagagatca agaagaactt
     8281 tctgaacgac atctctggag caagatggca tgcagacgta gacaggatca gaaagaactt
     8341 ttcgaagaat cagcggctca tgccgaagtc tgtcaaggag aaagagattg agaactttgc
     8401 aatcaacttc acatatgaca ctcaaagaat tgaaggatca acacttacca gaaaggaaac
     8461 tgccgatctg ctggaaagag gaattacgcc aaagaacaaa ccaatgaggg acgcgcagga
     8521 ggcggaggcc cacagggacc tgttctttga aatattgaag tcaaagaagg atctatcgct
     8581 gcaggaagtt acggactggc attggaaact gttcaacaaa accaaagcag accttgcagg
     8641 agagatcaga aaatatcaag tcgcaattaa aggaagcaaa ttcatgccgc caacccctgt
     8701 agacgtctat ccgatgctga cggaattttt cgagtggtac aacaagaaca aaaacaaact
     8761 tcatccagtt gagcttgcgg cgattgcgca tctgaagttt gtaacgatac acccgtttgg
     8821 tgacggaaac ggaaggatct caagactgat catgaacttc attctcgaca agaaaaaata
     8881 cccgatgctc gatattccgt acgagggaag aaatagctac tacaatgcgc ttgagaggtc
     8941 ccagataacg aagaaagaga gggtatttct ccagtggctc gtcaagagat acgtcaaggg
     9001 atatcgccgg tatctcaagt aggccatttt gcttcaattt cagatgaaaa aatcgcatgg
     9061 tacgagtaaa tttttacccg gaactccccg ggcccgcttt atctgtcagt ccagacccat
     9121 gctatttttg atgacaattt tgccatttgg aaaattgtgg tatgttgtta aacttcaata
     9181 caaaataatt caaccaaaat ttttgattag aagatccatc ttaaatagaa acacgtatta
     9241 tagaagtcta gtcatggggt gcgaatgtaa tacaaacatt ggtattcttg cacaagctga
     9301 aatcttaagg cgttctaatc ttggcacatc ttaatgcaaa taatacgttc atactagttt
     9361 tttttggtgg aattgtattg ctgtctggtg tacttcttta ctatatagaa tatgcaataa
     9421 attcggctgt aaatttactt ctggaaacaa ttacgattgg tttttggaac aatgataagc
     9481 ctgtatttgc tgattatcaa cttcctagca tcctaattgg aattggactt atctgtatca
     9541 taatgccgtt tttaatatat tttggaggca taatagaaaa aattctcaag gaaaaaactt
     9601 acagcataaa agattccatt attttaacag cagcaagttt tattgtgatc cgatttgttg
     9661 attttgctac aacaagccta gaaggaattg atagaaccgt gccatacaca accatgggca
     9721 ttggatttat cggcataata atgcccgttg caatgtttct tggtttcata atgcgcaaca
     9781 gagttcgtaa caagaaaact ctgagtgtaa aagacctttc caagataatc cactcgcttt
     9841 ccgcaaaaga ttttctctgg attatcagtt cgggcgttac gataataata cttggttatc
     9901 tattaggtaa ggtatctttt gacgataaag cagatgttcc tcgcgtactt gctgattttg
     9961 gttatgtcta tgtgataatg ccgctttcaa tatatctgcg cgacaccatt cacaaaattc
    10021 gcaataaaaa gactattggt gtaaaaaata tcattatttt atttgccgca gggttggcaa
    10081 gtgtcggttt tattggtctg aacataaaca tgatgccaaa tcaggctttt agtattgata
    10141 ctgagccaca tgtgggcgac aaggtacttc acgttgcatt atactttctt ggcttatcct
    10201 gcataataat atcacttgca aaagtctcaa gtatgatcat aacgactttt aagaataaaa
    10261 atcgaataaa gatcaaaaat tatccggggg caataatgga atatgttcat aaaaatgacg
    10321 tcgtaaatga gattaagaaa atatggaaat aaaatgaatc cttatttgtc tgtagcatac
    10381 tttttttaat cgcggcagag cgtagttctg tttatcgcga agaatcaaac tcgaattatt
    10441 cccttttcca ccaaatactg gattccctta atgaaatcac tatcagatat tgatccttcg
    10501 gaccaccatt ttgcattgct tttgatccaa tctggaatct tactttctga cacctcgtgg
    10561 gcttgctttg taagatttgg aattttcatt atgccatgct ctatcatgta ctcgattcct
    10621 tctgttaaat ctgactcgtt gctcatgcct tcagaatacc atctggcgtt gcttctgacc
    10681 caatctggta ttattgaaac actttctgac gatttggcgg cggaattttt tccaaggtct
    10741 ggttttctaa atccgttctc accataaatc tctctgactt tgtcaatgtc aatttgttta
    10801 atctgttgtt gctcgtaaat gtcagctcct accattacca taattgaagg gtatgggacc
    10861 acaaactcat tccagtatgc atctggaatg tcatctaaat aataatgacc aagacccaac
    10921 gcatggccaa actcgtgccg tactgtgttg ccaatctgca ccgagaggat tacgtcgttt
    10981 atgtagcacg gtttgtagta gatgtattgt tcatccctgt gatcctcaca atagtccatc
    11041 ttttgataaa aaactgaaat cagccgaaaa tcattatccg atccatcctt gttttcagca
    11101 gctccgagtg ccctatgcca cgcttcgttt gtatttggaa ctggcaaaaa ttgtatcaga
    11161 acagaacatt ctgccttatc aaaagatctc tgttgatgta aaggcactat ggaataatta
    11221 atttcccatt ttgacatgtc gtcacggctt cgctcccttt cttttagaag actttcccat
    11281 tgatcaagtg cgcttttggt ttcactaacc attgattttg tgattggacc gaaaatcatc
    11341 gtatctgaaa cataatcggg ctcaaaaata caaacattgg gctgccccca ttttagggga
    11401 gtgagggttt catacgttac agttcgtcca aacgatggct gaatggatgt taaaacaaga
    11461 acaaatacaa aaaagactgc aattataatt ttcaatgaaa atctacaatt aaggttgtaa
    11521 ttaagatctc gccaacgaat cctaaaccac atattatcta atcactagcg aaatgggttt
    11581 tttcggtttg ggagaacaaa ggagatgagg ggaagatttt tgcaagatat ttcgtgtttt
    11641 aggttgtcgc gagcttgatg cattcttgac cgcagttatc acagacccaa ctagtggatt
    11701 ttttagtgtc gatttcgtct ttttctgttt taacaagggt ttgaaattta gattctttca
    11761 aaagttcgcc acaattctcg catctcattt tgtatcatct tgttcaaatt cttttttttc
    11821 ggattctttg gataaatcta cgttaacagt tgctgtttgt ttacgtctaa gtttttcgac
    11881 tatttttctt aacatgcgca gcttggtgct cacatcaata taaaggcagg aattttcaga
    11941 attaagcaga atttttcaga cgtagctgag tagttaattc agtccttcct tacagatgtg
    12001 caaactcaca ccactacgct gatagtttac tacgcctatc aaacatcatc gttggattta
    12061 aggtaattca ggctgatatt gctaaaacgt ttcactttgg ggttttacct gtagtactga
    12121 acatctattt tctcagcaat tttgatctct ttttgcagct tgactttttt tagcgcatcc
    12181 tcaaaatacc tcattgtcac tttggtgccg cctacctgtt tttctgcttc tttctgatcc
    12241 ggatattttg ctaaaaattc atgtatgact agggatgttg cagtgtttgc gattgcggca
    12301 acgtctgcac cattgagtcc ttctgaggca ttagctattt tttcgagatt gacataatcc
    12361 ggattctcat ttgttccact gcttgcctcc ttatcgaggg gaattccaat cgtgcttatt
    12421 tctagaattt tctttctacc ttcattgtct ggcataggta tctgtattgc cttgtcaaat
    12481 ctgccaggtc ttaggagcgc agggtcaatc atgtctgctc tgtttgtagc ggcaagtact
    12541 actacgccat gaagcgattc tattccgtct atttccgtta agagttggct gacaaccctt
    12601 tcagtagctg acgagtctcc tcctaatccc ctaacagacg caattgaatc gatctcatca
    12661 aaaaatatga cacacggtga tgcctgtctt gcccttcgga agatttcacg tatccctttt
    12721 tctgactctc ccacccattt tgataaaagc tcaggtcccc tcaccgaaat aaaattggcc
    12781 tcgctttctg ttgcgactgc ttttgccaaa agcgtttttc ctgtaccgct tggaccataa
    12841 agtaaaattc cgcggggatt cttgtagcca atgttgttgt agaatgtggg atatttcatc
    12901 ggccactcta ctgcttcttg caactcgcgc tttacggctt ccagaccgcc aatttggtcc
    12961 caggaaacgt ctggattttc tatgtatact tctctcatcc ctgctggcgt tatctctcta
    13021 aacgcattgg tataatcttc agtatttact acaagcttat ctaaaatctc gactgaaacc
    13081 ttttcatctg ccaagttaat ttctggcagt attcttctaa gacatttcat tgcagcttcc
    13141 ttgcaaagat attccaaatc cgcgccgaca tacccatgac ttatggatgc aaatttttcc
    13201 aagtcaacgt cgtctgataa cggcatattt cttgtatgga tggacagtat ctcatgcctt
    13261 cctttctttt gcggaacttt gatctcaatt tctctgtcga atcttccagg ccttctgagg
    13321 gctgggtcca gtgagctggg tctgtttgtt gccgcaataa caattacttt gcctcttgcc
    13381 gcaaggccgt ccattagtga aagcaattgc gaaacgactc ttctttcgac ttctccggac
    13441 acctcttccc tttttggcgc aatagagtcg atttcgtcaa tgaagataat gcttggggat
    13501 ctttcccttg cttctttgaa aatctcccgt agtcttgcct cgctttcccc ataaaacttg
    13561 cctattattt cggggccgga aatgctgatg aagtgaacgt tgctctcatt tgctactgct
    13621 tttgccaaaa gcgtcttacc cgtacctgga acgccaaaaa gcaaaacgcc ctttggtgcc
    13681 tcgactccta gcttctcaaa aatctcagga tgtcttaatg gtagctcaat catctcacga
    13741 acctttctta tctcatcagt caaaccacca atatcctcgt atgatatttg tggaatgccg
    13801 tgcaaggact cgtttttttc accaacaaaa aatgccgttt tctgagtcac aattacggca
    13861 tctgcagacg gcgtggtttc cagtacaaga aaggtcaact tgcctccaaa atatctaacc
    13921 gtaacataat ccccttttgt cagcggagtg ttttctagcg actctgcaag gtatctctca
    13981 tcaatttggg gggctgcctc aagtggcgca actatgattt tttcagcagg aatagctgtt
    14041 atctttcgaa cctctacttt gtcacctatt gcaattccca aattgtttct tgtaacgccg
    14101 tcaactcgaa taatcccctt gccttcatcg gacgggtaaa gtggaaggca ttttgcaacc
    14161 gttctttttt ttcccttgat ttccaaaaca tccccagttg ctgcaccaag cgaatctgta
    14221 gcttcataat caagacgtgc tgttccgttt ccaacatcac gagaatatgc ttctagtacc
    14281 ttaagcgaaa tagaattttg gtttatgtat tctcaggtcg tatctgacca ttataccttt
    14341 gttattgtac aaaactgcct ccgttgtggc ggtagtaaca atgtcgtgta gttgtgagtt
    14401 ttagcggaag tgggggctcc aaaaagaaac tgtcttgcca aacatgtcta tccaccagtg
    14461 gagatagttg tttcctattt tcatggcatc tactgccatg tctgcttgga attttttgta
    14521 actggcataa atgtccaaca gatctattgt gctatttacg ccgctctcgc ataatcttcc
    14581 gtacgaattg acaatttttt gatctatccc aagcctatca aaatactgct tttgccaaat
    14641 atagcacgtg ccaaacaagt tatctacaca atggagaaat tccgtatgaa cttggctgta
    14701 ggcctgaaga taaatcggtg tctgatattc tacctttgta atcagtttgt ggatactatc
    14761 tctccaaatg tcacaaacag aatggtactc gtttgttggt tggatctgat ttttatcgtc
    14821 attttctgca atgccttccc attctgattt attggactct atagactcgg catcgcttat
    14881 ctgcatacaa ctgtatgtct tacaaaatac ataatgtcaa gtatgtatta aatgaacaaa
    14941 ccttgggctc tctaatgttc tacgttgtct attgattcca atgtgaacta ttgccacgaa
    15001 gcttatcata aaatatgtca aaatgtagtg tgcttggccc attaagcttt gagcaatcaa
    15061 aacttgtatc aaaattttgc aaggatgttt tgttattaga tgatcaggtg gtgtttgtat
    15121 ctgtaataag ccggcatgga aggcagattg attctgtagc taggggtgac tcgtcattta
    15181 atgatctgac aaagcacgaa ctagaacaag tatatctgca acgaatgctg caaacgcaaa
    15241 tgataaagga ctttgatgac aagctaagca agctatctgt agcaatactg gaaagagagt
    15301 cttgcataga aggcatattt cccttttatg atggtgtgtt actagttgtg tttaactcac
    15361 atccacaaat actggacagc gtcaaaaaaa cctcaaagtc aattaacgag tttaattttg
    15421 aaatagccaa atctggtgtc acgtgctaaa ttaatttttt tcaaagaaat tttacagcgt
    15481 atttctcatt tagaattttt attatggcgg tatcaaattc ggatacagaa atgacatgaa
    15541 gggccaggtc aaaatatgta atatctttca tcacatgatc cgccattact ctttgattcg
    15601 cagcgtgtca taaaagactg ccacaaatag aattcataga ttaagttgat ctacggattc
    15661 caagtaattt tagaattttt ctccaaaatg ttttcggagt atcttgaatc ttactcatgc
    15721 cctttgaact tcttcttggt gtagcgcttt gattactgag gcaatgcacc tgatgcaaaa
    15781 tgcctcgttt gcaagactag ccttttgggc tctcatagta ccaattagca cgagttttgt
    15841 aataagaaga tggacaatag atttcagatt tttcggatca gtatggaatc aaaaaagcga
    15901 tcaacttggt gatcataaaa taaggagtct ggcggctatt tttttgccag ttggctagtt
    15961 gtcttgtgtt gtttctcagt aatccatttg ctaatacagc gaaaaagata caataatcca
    16021 aaacatctga taaacaggca gactatggtt gaaagtaatt acgacatact tggggtaaac
    16081 accggagcgt cacaaaaaga gatccgagac gcatttagaa ggctagtgct ggatcatcac
    16141 tctgataggg gtggcgaaga cgaagaaaaa gtcaaaaaaa taatccaagc atacgaggat
    16201 ctcaaagtag gtaagacgta tccagacagc gaagacgaga gactaaaaaa agcccgtgtc
    16261 tacaccggtg actctgagga agaaagaaaa aagcgcaacc tgactttgtc aggagatgtt
    16321 gcacgggaaa tgaagcttgc agaagactgg gcagaacttt tgaacagaaa ccacgcaaca
    16381 ggatcgaggc tttttggctc aaaggagctt ggcgaagtag agtttgagcg aaaagaaaca
    16441 ggcgagcttt taatcaaggg gaaattttgg gcaggccacc tgacctatgg ggggccaatc
    16501 ataatggcag gaagcataac tagcccctac tttgcgcaac aagaagaaac caagacgacc
    16561 attaccgtaa ggaatggcaa ctttatgatg cttgatccgc tccaaaacaa ctttacaata
    16621 gagcacgggg caaaaatcat agcagaaaac ggtgacatca tagttgggaa tttgttgggg
    16681 ataaaagaaa tactccaaga cccgcaaggc cgagtcggaa tatacatcac aaaagaacac
    16741 tttacagaac taagggcccc aaaaggcaaa gtcgttgcag gcacagcaag agaaacagta
    16801 tttttagatg cagacactgt ggtcgtaaat aacttgatta acaatatcaa agtcagggca
    16861 agacacatta caatttttgg gagcacggta aactacaact gcgagcttga gctgagggca
    16921 gggggctcgc tggtatttca cgatgagggc tccggctttg cactctcaga cgatgcgtta
    16981 gtaaagctcg aaaatgggaa atggttcaag ctacaagaac taaaaacctc aaacatggtg
    17041 gggcatggaa ggcagataac ctacgaatat ctggataatt tgggcaagaa gtcagaaaaa
    17101 tcccaagggg ccaagattaa cctcggtaag ttcttcaaga aaaaatgatc taattggagc
    17161 taagcattgg ctgcacgggt tggggatatg acggatgggt tggaccattt tacccaaaaa
    17221 gcctgccaaa ttcaaaattt ctaaaccatt attcggagat ttttgatatc accgaggtaa
    17281 actctacatt ttacgccata cctcaattac aaattgtgaa aaaatggtat tctgataccc
    17341 caccacaatt caagtttacg gcaaagatgc caaaaataat cacacatgaa aataggttgc
    17401 aaaatcttgc gccatatctt gagcaattct tagagtcaat gaaaccgctt ggctcaaaat
    17461 ttcttttatc agtggttcaa ctgccaccgc cactctcatt tgaggatgca aaaccaaatc
    17521 tggagcatct ggaatcattt tttaaaaatt cttcctatgt gatagaggga aggcatcaaa
    17581 gctggttttc agaaaatgca ttgaggtatc tgactgacaa aaaaatctgc cttgtctgga
    17641 gtgatgtaca gggaataaaa aatacagcgc cacttacttc ggatcttgtg tacctcaggg
    17701 tgatcgggga cagatcaatt cctgaaaaag agtttggcaa agttttgcaa gacagaacaa
    17761 ctgatcttaa ggggtgggca gaaaagatta atcatctcaa ggataaaatc acgcttgcag
    17821 tcataatggc aaacaaccga tatgaggggt ttgggcctgc cactgcaaac aaactgcgcc
    17881 ttttgcttgg attagatgcg attgcgtttt ctgatacgag acagaaaaca ctggaaaatt
    17941 ataagtagcg atctaaacat acgtttgatt ttgcatttat tatctcacaa acgttcaagc
    18001 tagccgtgat catctttatt ggccgctatg aagaaatttc aaacaggata gtatcaagta
    18061 agacaacact gatgcgcttg tactgggtcg gtgggctagg aacggtgttt ccttcgcact
    18121 gcttgaatta ctatcctgtt attatttgaa cgtaatttta cacatgttta ttatttttga
    18181 ccggatactt gaaattttaa tgaatttact cttatcacaa ttatagctaa acaaatttta
    18241 ataattttca taatatgtgt tatatttgtt tgatgcgtaa tatctcagtt gaaaagcgca
    18301 acaaatctct gcacaaaatc acaaaatcct ttcggtggta cgtatggtta gccgtgcaga
    18361 aatctatcaa atggccaaga ctggctgtgt tgcaggactg gtgggcggat tcgcgctttt
    18421 cagctcgttt ttctggatag atagtgaggt tggtgtacca tttggcacat tctacaaggc
    18481 ggttggaatg gttgtaggcc tcgatggtct tgcggcaatc gcatttggat tttttgcaca
    18541 catgcttaca gctgcattaa ttggagccat attttgcata tgctctacat tccacaaact
    18601 cttgcacata tctagcgttc caaaaggaat tatcgctggc gccgtaacgg gaattcaagt
    18661 ttatgcaata ttctttatgc caataacagt gtatgtgatg tttccaatgg tgtcggcgca
    18721 agcctcaggc ctgtctggct ccgtaacaca ggaagacatg caaattgcaa aaatgttggt
    18781 ggatacatcg gatgcaatcc tatggggctc acttgtactc cacgttttat atggcgcagt
    18841 aatgggtctg tttagctcaa tgatgatgta tgaggattac cacatgaaag aaaaaagcaa
    18901 aaaagaaaaa aaggaaaact ggaaaaaatt cgagagtgaa aactggccat ctacatgacc
    18961 accactgtct cgcagctctc ctttacaaag agggtccaga tcggatgtgt tgcaggacta
    19021 gtaggagggt ttgcgatttt tgtcagcata tttgtaattg atctaagctt gggggcaagc
    19081 cagggcacat tctacaaggt agttgggctt ccagctggaa ttgctggaat ttatgcgacc
    19141 ctctttggaa tggttctgca catgctcaca gccgccctga tgggaacgat ttttggcatt
    19201 ggctcaggca ttcacaagtt attggaaatc tcgtcactaa aaaaaggcgc agttgccgga
    19261 attgtaacgt cgctagtcgt gtttttggtg tttttcatgc caattagcac atttttgata
    19321 attccactct tgcaatcaag cgccattgaa ttgggcgagg caagccagct tcttgtaagc
    19381 atggagctga ttatgattgg ctcgcttgaa ttacacgtag tgtttggaat ggtgatggga
    19441 atattttttg cagttgcggt tcagcacgaa tcaaaaaaac tagttgaggc aacggcggtc
    19501 taattgctca gaattgtagc tatctcggta ataattggaa cctttctaat cggaacatat
    19561 tacgtagtcg aagcatcata catacaacct atccaaagtg acaggattgc aggtgcacta
    19621 ggggaggttc aggtgggcct cacatatgag aaatttgtat cgctgagtga gggcgaaaga
    19681 tcagttcttg tccaacaaat gccgtcccat acaattacaa tgttgcttca agaagcaagg
    19741 aatcacccga gttttattgc ggaaaaaaca gatgtcataa aagaacaatc tggcttaagc
    19801 gaaatcagac ttgtaaaact gtctcaaatt tctggactaa agggatatga ctcaaaagga
    19861 gaggcagcaa taataatttc tggcgacaag gcctttttgc gactgcaaga atttggtgta
    19921 acaagtggaa ttgatcaaca cttgtatctt acaaaagatg gaacaattgc cacaggaatt
    19981 gaccttggaa gactcaaggc ctcccaaggt gatcaatact acgacattac aggaatagac
    20041 acagaaagcc acaatgtttt gataatttac agcaaggcct ttgataccta ttatgcacac
    20101 gcaaaatttc tgaagacgca ataatctgtt cgctaaatgg ttttgcctaa tttttaatct
    20161 ctctagcaat caattattat cttgcctcag cattagagat catcaggcga tgaagaggag
    20221 ttagagacat gataaaacat gattgtattc agtgaatctg agtcctgtaa aattttgagg
    20281 gttggcaggg gtatagaacg aactagccaa agattatgca atgctcgcat tcacagtttg
    20341 ggatttgttt tgattttgca aggcactttg catgctggcc tgcctgacat tgtacgcaaa
    20401 tctcatctag gttgtccacg ctagaatatt tttttgactg atcaaagcca aaaccaccat
    20461 cttttgggcc aaccatgcta tggatagtgc agttgctgta tttttagatg gtgatcttct
    20521 taggatttgt cctcatcgcc tgaatctttt tgtttgtccc aaaggttcat tgttcctctg
    20581 agcataaagg cgcctacaat tatggttaca attcctacta cgacaaacga aaaaaccttg
    20641 ataatggagc ctagatttgc caagctattt gacctcaaga cagactatgt tttcatgtcc
    20701 tgtaattctg tatgtttctt catgcgcgtc ttttccctca cctgtgtaga cgtcgatgtg
    20761 ttttccaata attgatgtgc caatatcgga tgccaaaaac acaacttcat tccaggggga
    20821 aagaagggtc gggatcataa cacgagaatt ttgaggaatt attgatgggt caactgctat
    20881 catgtgtgat tcaaggttgt tccctgtggc atccaatgga aaatcactta ggtgaaatga
    20941 ttcatcaaac cagccgagat acttgcctga gagtgtcttt ccccatcctt ctgttttgat
    21001 ctctgtaaca aaatcctctc tgaactgata agaggctcca tcaacattta ctgttatgac
    21061 cttaccagca tagtctgatt cggcaggtgt aaagtatccg gtgatgtacc aatgggagat
    21121 acagtttgag aattctgaat gaccagctga attttgttca atgatggaat gtgtaatcaa
    21181 aaaattggta atggattcat cctgcgagtc tgtcgatagt ctcattactc caactttttg
    21241 caagtatgtg atggcacttt caaattcttt tgcagatatt tgctcgtcct taaaatattg
    21301 gtatactacc aaaaaccact ttggaatgaa aaggtcttgg cttgcaaatg atggcaacat
    21361 gacaaaaaca atcatgaagg caaacagcat tacaaaaaca cgatctgaca atacgtaatg
    21421 gctttgtaat tgtcagataa aaacacacgt tgtcttgcag catcctttca atgcatttca
    21481 attaagatga gctactgctt tttaataatt aggggcaagt tcaagatgaa attgagccta
    21541 tcgcagccaa gaactaaagc caattcatgc ctgttttgtg gggaaatact caaaactcca
    21601 tcaaaatgtg cctactgcgg tttcaagttc tgccaggatc atctattgac agacgaccac
    21661 caatgtggca agacaaggta tgcagagtat gtaagaaaga caaatgatga ctctgtccca
    21721 aacctagcaa gcggcatgtt tgtagtggtc tgcgacacat gcggttacag atcaacaaag
    21781 gcgaccctca tagaatttgc aggagaggaa ctcatccaac acatgcaagt aattggatgc
    21841 tcagaaaata catttctaga agagattggt gatgctacgc catttgattc tcaaagcgtt
    21901 gaaataaaat caaacgatca ggatctagta actagggttg gaagcacatc acaaaacaac
    21961 tccgaaccaa aggacggaat cgtagaacaa cttgcaaaac ttgcgtcatt aaaagaaaaa
    22021 ggtgtgctat caaacgagga atttttgttt atcaaaaaag aaatcatcaa gaagctaaaa
    22081 tagtaaacag atcttttaag attgagaaag atcgtcaagc ttcttttttg tctctgcatg
    22141 atctgctcgc tccctctgca acgcaatttt taatgtttcg agctcttttt gggcggcaag
    22201 aagcttagtg ttaagtgatg cgacaacgga gcttgctgcg gcgacgatgt tttttggcgt
    22261 tgcacggtca ccgacatttg acatctgttc ttcgatgaat tttagctctt tttttgcaag
    22321 ctctatttct tttttctttg attcgtattc ccttcgtatt tcctcgattt cttttctagc
    22381 tgactccagt tcggctttct cctttgtgat cttttgtcct gcactttcat gtgcgcgctt
    22441 gttttcctcg tacgatgatt ggagcgtttt tatcttgttt ttcagctcct cagattctgc
    22501 tttgtacttg acatattctg attttatggc aaccaactct ttgtttgccc cttcaagctc
    22561 acttagcaag gtcttttttt catcatattg gccaatgatt gattctatct ctgatttttt
    22621 ctcatttagc tccctttttg cagacatcag ctcaccgaca acatgatcat attcctcttt
    22681 tactttggca agcttttcag aaatggagtt taggtggtca gtctttgatc caagttcacc
    22741 ttggagatac tctaactcgc ttgtaatctg ctccttttgt atctcctcgg ggctctttat
    22801 ctgcacaggc tctgcttcct tggcctgccc gataggctct tcttcttttt ttgctcttct
    22861 aaaaaatccc atgactaaca ccacttagat gaaacgacgg atatttcttt catttccgaa
    22921 taaagcggag ataaaatcct agtccgccta tcaaaaaacc aagaacgatt agtgccaaac
    22981 caaactctgg gctctccgaa aaccaagcag gagttattat gaataataac gcgcccaaaa
    23041 taattagcaa gaacgcaccg ctcttttttg gcctgttgtg ttcgctgtga gccattttac
    23101 agatactcgc taatcaattc ggctactttg tttcttccaa tgtctattat gaaaggtcca
    23161 atttttggtc cttggtttgc agaaagaatg atcctgtaca ataccttgaa aaattctcta
    23221 ggttctatgt tattttcttt agcaacattg taaattttat tctgcaagtc ttgaggatcg
    23281 tcatttgcgt ataaaatttc aactagctga tttagagctt ttttcatttg gttgtctatc
    23341 gtcatctgaa tcaactctac tccttcaaaa tcatccgcat aattgcctgc aagtgtgatc
    23401 agatctgcta tatgtggttc tggatttttg attgccctat actcaatcaa ttttttcatt
    23461 actagattta tcctgtcttc tttgaatatg cgacacagct cgattagtaa cctgtagctt
    23521 acatgaactg gaggggtctt tggaggattg agcaagttcg tgtactcata gagccccttt
    23581 gatttgactg tctttgcctc attgtcaagc ttgattttgc caaagtaaat atcttcaaga
    23641 tcattatact cgtccattaa tgtcgggatg tcttcaaatc caagctccct tgcgccagta
    23701 attcttttgt acaataacaa aagcattgac tttggcgtgc cgtatctgaa ccatttttgg
    23761 gatgttacga cgtttccggc agacttggaa atcttttttc cgtccttgtc caaaaacatc
    23821 tcgtatttta cgtggtgtgg gtgtgggtgt ccaagtatct cgtctgagac ccagtcattt
    23881 accttgactg agtccatgat gtcctttcca tacgcttcaa acctaatatc aaatgcgtgc
    23941 caccttgccg caaactctac tttccaagca agctttccaa ggtcctttga gatgtctgct
    24001 tctccctcat ggctacaccc ctggagtatc tgcgagccaa tttcggcatt agaacatctg
    24061 tacttgactt tcatattttc cgcatcgtac tcgtatgcct gcgcagtgta gaggcggttg
    24121 cattttgcac aaactggata gtatggcaaa aacttttgat atttttcctg tcctaccatc
    24181 tccgcaatct tatttccaat tttttctgta tttgtaagaa ttgtatgaat ttgatttttt
    24241 agaagtccct gtctataggt atcaaatgca cgctgaaatt tgtacttgat tccaagctta
    24301 tcgagaccat caagcaaaag accgctcatg tgctctccgt aggaattatg acagctgcca
    24361 aaaggatcag gaataagaga aactggtttt gcaatgtgtt cgccaagcca ttctggcaag
    24421 ccttcaggta tctttcgcaa gccgtcaaga tcatccgaat acgcaataag ttcggactga
    24481 aaaccagact cttctagggc cagcttgaca ccataggcgc gaactgcatc acctaggctc
    24541 ccaatatgtg gtatgcctga tgcaccaagg ccagactcta cccgtaaaag actggtatct
    24601 cgcccaatct gtttttcccg ctcaatcatc tcgtatgcga gcttgtcaat ccacgttcct
    24661 ttaccaataa ttttttgatc agcctctgac atcttgtaac actaaaccaa cagttttgcc
    24721 atataaggac catccaacaa ataaccaagc ttgtgataat actgtcttgt tccaaccgcg
    24781 ctgatcacca aaagtttttc tgcgtcaaac tcttcttttg caattttttc agcttcgctc
    24841 atcaggttct ttccaagacc agagtgttgg atctggccgt cctcgcgctc gccaatctta
    24901 agtgacctcc catagatgtg cagctccctt acaatacagc tgttgccagt tacttctttt
    24961 ctatgtgcca tctggcttgg ccttctcaat ctcaaaaagc cgtaaatttt atcgtctgaa
    25021 tcatcataag acaaaaagac ctcatctcct ccagacgacg tatactcatt acggtgcagg
    25081 aaaatctctt tttgcggctc atttgtcagc ccgatttctc tacatctgat gcacttacag
    25141 gacaggtttt gctttttcaa attactgtgc actatttggc gtaggttgcc ggacttgggg
    25201 ccagcaatga tttcctgggg tgatatttcg cgctgaactc gcatgattcg cacccagcgt
    25261 ggaatgttct tttttacctc tgttagaact ctgatcatat cctcatcaga ataaggcaag
    25321 tactctcctt gctgatattg ctggtatagc ggcgtgtgct gcagaacaag cgacgggtaa
    25381 atcttgagca tgtccggctt gaggtcatcg tcttcgaaga gttttttgaa gtcagcaatg
    25441 tctccatctg ggctcattgt gggaagtcct ggcatcatgt gtgcgacaat cttgtatcct
    25501 gcatcttttg atatctgaaa tgactctaca acatctgaat atgtgtggcc cctgttgaca
    25561 atcttgtaca cgttttcttg cagggtttgg acgccgattt caacccgtgt tactccatac
    25621 tgtagcatca agtccacatg ttcttgtttg caatagtcgg gctttgtctc aattgtgaat
    25681 cctacacttc gaatgtgtgc tttttcgtta ttggtcttag attcctcaag atcttgggaa
    25741 tcaaatccat ttagtgcatc atagcaggac ttgatgaaat tttgttggta atcttttggc
    25801 atgaaaagaa acgttcctcc aactatgacg agctccatct ttgtggggtc atgaccatat
    25861 gcaaccaatt tctcaatctt tgagagaatc tgtttttttg gatcgtactg gctttcaatt
    25921 gcgtttatcg tggccggttc gtttcctgtg tagctgttag gtgaattgaa ctctattcca
    25981 ccaggacaat aagtgcatct gccgtgcggg caggcatatg gcattggcat gagtgcaatt
    26041 actgccacac cggatgctgt cttgattggt ttttttagta gtattttctg caaccgcgtg
    26101 tagtctccgc cgcttaccgt ggataaaatc tcatagtttc gtggaatgcg ttcaagtgca
    26161 tattttgtgc agattttttt gatttgtgct tttatctgat tttttgtagg agagtctatt
    26221 gtaagtagat cacgcgaaat ctctacgcag gccttggcaa aaatcgtgct tgtttgttgc
    26281 aataacttgc aatatgtggg tggtcttaaa ttctttcaaa aacgactcta gcgtcttttt
    26341 ttggcttttt ttgatttggc cttttttgcc ttgatttttc ccgttttggc cttttttctt
    26401 ttgtttttct tggtgtgttt tttccttgct ttattggtaa gagttttagc tgtctttggt
    26461 tcttctagtc ctgctcttgc catcatgttt tttgtttgaa ctagttcgat tcgtgttttt
    26521 gcactaagat aagctagctc actgtttggt gttggaaatg agcctccgtg ttctttaaaa
    26581 cattgcacat gaaacacctt tcctttttga aaatccatat ctgcctttga aacaccttgt
    26641 ttacacacgg gacacatgtc aacaaagttt ttgtcctcaa ccaatgatgc tcgactctat
    26701 tggactaatt tattattttt ctacaaaatc taaactcgat ctaattctgc gattctggat
    26761 tcctatgaag ctcttttctg tactccatct tcaaccaaat aggcgtgatg atgctagtga
    26821 ctgccaccat tagtactatt gtggtataga catttgaggt gagaacgcca cttgatagtc
    26881 cgactccggc cactatgagg cctacctcgc ctctcgatac catgccgatt ccaactcttt
    26941 ttgcttgtgg cccatttcta agaaacagcc aagctggaag accacatccg aacatttttg
    27001 ttgccacggc aacaccaatt acgactgcac caatgaggag aatttccgga tttgctccta
    27061 tccccctgaa atccacctgc gctccaatta ttgcaaaaaa gagtggcgca aagatcaagc
    27121 caagcttgct ggcataatgt tccactcttt caaagagctt agttgaagat agtgccatgc
    27181 ccactacaaa tgcgccaagg attggcgaaa gcccaattac tgcagctacg ccagcagcac
    27241 caaagaatgc ggcagtaacc acgccctcaa tacttccacc agccttccat agtttggaat
    27301 tgacaagttt tggaaccaaa aatatggctg caactgccat ggctgcaaag aacgcgagca
    27361 ccttgatgat ggtaaatgac acatctacta catctattga ttcgccactg actccaagcg
    27421 atgttacgac tgacaagaca gcaattgcta aaatgtcgtc aacaactgct gcgccgatta
    27481 taagtcgggc ttcggggctt ttcagttttc catactcact tagcacctgc acggaaatcg
    27541 caatgcttgt tgcagtaagg gcagtagcta ctagcattgc ctggtatgca tggaacccaa
    27601 acagctgaaa gataaaaaat ccaacaacaa atggcacaac tactccgagc gtgccgacag
    27661 taaatgacgc cttaccgcct ttgatgaact ctcgcggagt catctcaagt cctgccaaaa
    27721 acaaaattac aattgcgcct atttcgccta ggatcttaac ttctgtgcta agctgcaaca
    27781 acgtctcgcc ctctattaca atcaatgatc ctattgcaaa tgggcctatg atcatacctg
    27841 ccaaaagctc gcctaaaaca attggaagct ttagtctaac aaacagctca gccatcagct
    27901 tggctgcaaa aagcaaaata catactccaa ttacaacttg aacaaaatgg gcctccaagt
    27961 cacatgcttt gcattttggt catataaggt agttgatgat taaaaatgat gagaggtttt
    28021 gtctaattat gccaattttt tagaattgac atagactcct tttctgtcag aaatgaagcc
    28081 aatatcctga cttttcatct tgtacttttt acaaattgaa atgattcttt gtgtttcttg
    28141 ctttggtgca accacgcaaa acccgatccc catgttgaag gttttgtaca tttcactctg
    28201 tgttactccc aaatcctcaa ttagctgcat tatctttggt ggctctggca tagaatcaag
    28261 cacaaaccct gtctttttta gcctcaaaag cttggtaaac gcaccacctg ttatgtttgc
    28321 aaggccgtgg actttgcagc gcaaaacaat ctccatgatt ggttttacat aaatctcagt
    28381 cggtgtcaaa agtgcatccc caagttttcc aaccccgctt actttgtcac taatggaata
    28441 tttttttagc aaaacctttc ttgcaagtga atatccattt gagtg
//
LOCUS       DASWKH010000079        26723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3185247, whole genome shotgun sequence.
ACCESSION   DASWKH010000079 DASWKH010000000
VERSION     DASWKH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 26723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26723
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3185247"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGA92_05710"
     CDS             <1..229
                     /locus_tag="VGA92_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEX9845945.1"
                     /translation="DYVKFLLKDAKTIVDIEANQTAQLGQLLKQHLERGADYYILKYT
                     GRPMTSTEIYESLKKIIEKKAQKRVVLTHGA"
     gene            219..1184
                     /locus_tag="VGA92_05715"
     CDS             219..1184
                     /locus_tag="VGA92_05715"
                     /EC_number="1.2.7.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018491 - 2-oxobutyrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0047553 - 2-oxoglutarate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX9845946.1"
                     /translation="MALKLGDYKTEVHNDWCPGCGDFGIVNAIQMALSEMQIPRHKAA
                     IFSGIGCSGKTSHFINVYGVHTLHGRVLTFAQGAKLANPEMTIIAAGGDGDGLGIGAG
                     HFVAAGRRNLDLTYIIFNNGVYGLTKGQASPTLKLGEKTKSLPSPNTNYNVNPIGLAI
                     ASGFTFVARSYAYDVRHLKDMIVAAVRHKGLAFLDVLQPCPTYNDINTRDWYSGMDRM
                     DEATQKPKPRIYKLEESKYDPVVHYDEESELNEKLTQALIRSLEWDTKIPIGIFYKNE
                     IISQYDRRIMDKIPNYLENPPASQVISEKGKALTDLSKILDSLSV"
     gene            1358..1483
                     /locus_tag="VGA92_05720"
     CDS             1358..1483
                     /locus_tag="VGA92_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845947.1"
                     /translation="MTKLVWTDENTKSVETYKASVRSGQTKPVVLSGTLREVIKR"
     gene            1833..2267
                     /locus_tag="VGA92_05725"
     CDS             1833..2267
                     /locus_tag="VGA92_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845948.1"
                     /translation="MNLTEANKIFRKSIIKGYFEPHLLNLDFSKSNIKHPTIPDDGLM
                     QSSLLHIFFDIDTGSDYPDGDEWFIAEFLFPYSIKIPDHVKGPDYFTALSLSEGKNFW
                     HHRELLRFKYGKSKKLEESLEFLDAKYKELHSLLEPLEKDIK"
     gene            complement(2260..2796)
                     /locus_tag="VGA92_05730"
     CDS             complement(2260..2796)
                     /locus_tag="VGA92_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845949.1"
                     /translation="MSDGYLTASVDYFGVSPWEAEIVYELFAERFRVLQTELEESDPN
                     FVSVLRLSIPLEFNEEFFKWFEYRRWDKVKFMFKEMKRRRGNDKALRIDINFLGMPKI
                     GFTLDVHERSWFDNALEKLDFVIELLPFQLNSVNIPHEISKVIYKFDVETRKWKLSTL
                     FADNKRFALVNNTWKVII"
     gene            complement(2847..3398)
                     /locus_tag="VGA92_05735"
     CDS             complement(2847..3398)
                     /locus_tag="VGA92_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845950.1"
                     /translation="MQEFLTRYPKTISFHDGKKHTISIDTKGVEQLTVIVKKSVEDLL
                     KILANEGFTKVKFEHKQESQIGDGFSLKLKKPWEMHLRLFDLKKGMVAIQAEVEVSRD
                     YLQHLFCQRTPVIYEIEAMLKKHQVEYKVWSDKVKNYVHMVFDNYKIKLTTPNIPVFA
                     WKPMLFVIGTIGMLYLWKYLNTI"
     gene            complement(3467..4231)
                     /locus_tag="VGA92_05740"
     CDS             complement(3467..4231)
                     /locus_tag="VGA92_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX9845951.1"
                     /translation="MTLSVIPIRVEKKQTSFDLYQSIVGSLGNVILENGDVLVVSSKY
                     ISNSQNRLIDLEKVRPSTDALVLSKKYQLGSKFSEVILRESDSIFGGIAGFVLTSSDN
                     VLAPNAGIDKSNVRKGTVILYPDNPYLIAEQLRRKILLNLGVSVGVIIVDSRLMPTRV
                     GTSGVAIACAGLEPVQDMRAQKDLDGNPLKVTLQATADNLATIANHKMGEGSESTPIA
                     IVKNSGARLTARKISPTEMAISSDLCVYVRGFQEKK"
     gene            4307..5575
                     /locus_tag="VGA92_05745"
     CDS             4307..5575
                     /locus_tag="VGA92_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9845952.1"
                     /translation="MNITEKILARASGKSQVAPDDIIFANVDKAMIHDVSGPGVIKVF
                     EKLKKEGISVDRLWDSSKIWVAEDHFVPSADKISAENIVKLSNFTKQYGIEKHFKYGM
                     GQYGICHTLSHEEALVLPGEVYIGGDSHTNTTGALGAFACGLGHTDVAYVLLNGKIWF
                     KVPETLYFKINGKLPENVMAKDLILKIIGDIGTDGAAYAAMQFGGTGVSDMSVESRLT
                     LTNMTTEAGAKNGIIEPDQKLFDYLAMRGAKNYSPTYGDKDAQYAKMYEYEASELEPL
                     VAKPYSPENIAIVRDVAGVELDKSYIGSCTGAKYEDLEAVAKILKGRKVKIRTEVLPA
                     AISIYKRAMENGLLKIFLDAGATVGPPTCGACCGAHMGVLAKDEVCISTTNRNFPGRM
                     GHIESKTYLASPLVAAASAVTGKITDPRDL"
     gene            5575..6060
                     /gene="leuD"
                     /locus_tag="VGA92_05750"
     CDS             5575..6060
                     /gene="leuD"
                     /locus_tag="VGA92_05750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402761.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9845953.1"
                     /translation="MAGKVIKYEKDNIDTDVILPGQYLKLHDYDEIAKHAMEGMDPNF
                     HARVKKGDFIVAGKNFGCGSSREHAPIALSHCGIMGVLALSFARIFYRNSVDGAFLLP
                     IEIDEQTYQKISDGDQIEVNVEKNEIKNITKNEVYKMKPFSEIASKIIAAGGLFKYKP
                     D"
     gene            6115..7521
                     /gene="leuC"
                     /locus_tag="VGA92_05755"
     CDS             6115..7521
                     /gene="leuC"
                     /locus_tag="VGA92_05755"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9845954.1"
                     /translation="MAKTLFEKIWDQHTVTEIAGRTLIYIDRHLVHEVTSPQAFDGLR
                     INKRTVRRPDLTFATMDHNVPTTNRSLPIIDQISAIQIQALEKNCKDFGIELFDLHSP
                     HQGIVHVIGPELGITLPGSTIVCGDSHTSTHGAFGAFALGIGTSDVEHVLATQCLVLD
                     KPKTFEIKVEGKRKNTHTITAKDIILSIIKKIGTGGGAGTVIEYRGQTISGLSMDERM
                     TICNMSIEAGARAGLVAPDEKTFDYLRGKKYVPKNYDELVDYWRKNLKTDEGAKFDKS
                     FTLNASDIAPQVSWGTNPAMTADVTESIPDPQEFAKGDKSQETAAKKALEYMDLKAGT
                     PITDIKIDRVFIGSCTNARLQDLIEAASIIKGRKVAPTVRAMVVPGSQQVKRKAEDLG
                     LDKIFKDANFEWRESGCSMCLGMNPDILARGERCASTSNRNFEGRQGAGGRTHLVSPI
                     MAAAAAITGHFVDVREWA"
     gene            7521..8093
                     /gene="leuD"
                     /locus_tag="VGA92_05760"
     CDS             7521..8093
                     /gene="leuD"
                     /locus_tag="VGA92_05760"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9845955.1"
                     /translation="MEKFNRVISIGTPLDRPNVDTDQIVPKQFLKLVQRTGFGKFLFY
                     DWRFEPDGHANEGFVLNDPKYTSSHILIVGENFGCGSSREHAAWALKDYGFDVVIAPS
                     FADIFYNNCFKNGILPITISKGTVAELAQTSSVIEVDLVTQTIKYDNKTVSFSIDEGR
                     KKTLLEGLDDIAVTLLHQDKIDAFEKAHAL"
     gene            complement(8081..8809)
                     /locus_tag="VGA92_05765"
     CDS             complement(8081..8809)
                     /locus_tag="VGA92_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9845956.1"
                     /translation="MKAVILAGGRGTRAKPFTDYFPKAMIPVDGKPLISHVVDYLSSF
                     KIISEIVIVADISGLGGQIKHYFAKMGPKKITFLQDSASGTAGDLLHLRNIVKNEPFV
                     LWFVDNLGAINLKKMHQNFVRQNSIACIATRQFRKEETGFAKVRKSKVMEFIEKPLVR
                     LPDCECTGIYILSGKILGLINKSKSKRDLNLSYDVLQGLSKKGLVSSFDIGKILWMDV
                     ESPTVLERKTDTVKKIISQMSHSA"
     gene            complement(8810..9256)
                     /gene="rpsI"
                     /locus_tag="VGA92_05770"
     CDS             complement(8810..9256)
                     /gene="rpsI"
                     /locus_tag="VGA92_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9845957.1"
                     /translation="MIPKTEIYFATRKTSKAHVYITKGSGRVRVNNTPAEMIGQETAR
                     EVILSPLEIAGDLRKKVDISVRVKGGGFMGQAYAAATAISRALTGWTKSKKDPKDHPL
                     PKQARTDLRKRINDFDKHLLSGDARRKEPKKFGGPGARRRKQKSYR"
     gene            complement(9253..9693)
                     /locus_tag="VGA92_05775"
     CDS             complement(9253..9693)
                     /locus_tag="VGA92_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9845958.1"
                     /translation="MDNAQLIVVDGTDHLAGRLSSNVAKMLLKGNRVIVVNCEKIMIS
                     GSKSNIVKNYKAFLEVASILHPEHGPYHPRRPDTMIERMVRGMLPRKKPSGQAALKRL
                     RTHIGVPSQFRSSKKIILDKAKITRSSANYTTMAELSREVGNWQ"
     gene            complement(9686..10033)
                     /locus_tag="VGA92_05780"
     CDS             complement(9686..10033)
                     /locus_tag="VGA92_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HEX9845959.1"
                     /translation="MTNQLVIGLVKELKIASSKNKAPIWSRLAQMAQKPGAARRIVNV
                     GKLNDVTGDNDVIVVPGKVLGTGDISHKITLCSFSISTSAAKKITTAGGKIINHSEMI
                     SKFPTGKGVKIIG"
     gene            complement(10066..10704)
                     /locus_tag="VGA92_05785"
     CDS             complement(10066..10704)
                     /locus_tag="VGA92_05785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HEX9845960.1"
                     /translation="MPSLEIISQDKQRMSVKLKGVPLQYANALRRVCLNGVPVFAIDT
                     VDVIENSSVMSDEVVAHRLGLIPIKTDLKRFNEPAKCPCQSKTGCSNCRVMLVIDSGD
                     TDTTRTVTSAEFSSEDETVKPVSDKIPIVQVAPGQKLKLEAYARLGRGSDHAKWNSAN
                     ISVLTHTDKQDEYVLTVESTGALSPEQIITTGIDELAGRLEEFRQVVAELKS"
     gene            10791..11477
                     /locus_tag="VGA92_05790"
     CDS             10791..11477
                     /locus_tag="VGA92_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HEX9845961.1"
                     /translation="MTDVTVIRHSVAGEKFEILVKPDPALEYKMGKRKDVSSVLVSDE
                     VYTDSNKGTRASTEKLLKAFNTQDTNVIIEQILKKGDLNLTTDQRRKMTSEKRKQIIN
                     YVAKTFVDPRSHLPHPPLRIEQALEQARISIDPFKNTEEQARDVVEKLRTIIPLKSEN
                     MLLEITVPAQYAAQSYSVLKSNGTLKKEEWQNNGSLKAILEIPAGARANVIDRLGSIT
                     KGSATVEMVK"
     gene            11477..12157
                     /gene="rrp4"
                     /locus_tag="VGA92_05795"
     CDS             11477..12157
                     /gene="rrp4"
                     /locus_tag="VGA92_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HEX9845962.1"
                     /translation="MDDIQRKYVIPGDVITTGPYRAEENVHLFGDKIIATTVGISEIY
                     DSGVRVIPLTGIYVPRIDDFIIGMVKSHTSLSWELDINACYPGILPAQDVFGRDFNPK
                     VDELTSRLKRGDLIAARVANSDRSRDPLITIADRDLGKIDDGELIKISPTKVPRLIGK
                     RGSMIQTIEGATKAQITIGQNGYVVVSCEEAEGLLKALDAIKTVEEQAHVPNLTEKIQ
                     EMLGSNSE"
     gene            12161..12895
                     /gene="rrp41"
                     /locus_tag="VGA92_05800"
     CDS             12161..12895
                     /gene="rrp41"
                     /locus_tag="VGA92_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HEX9845963.1"
                     /translation="MGVRKTDIVLLSDKGIRCDGRSIDEPRRIMIKAGVLKNANGSAY
                     IEFGENKILAGVFGPRDVHPKHLANTDRGILRCRYHMQPFSVGERKNPAPSRREIEIS
                     KVIKEALEPAVMLENFPRTVVDVFIEVLQADGGSRCAALDAAAVALADAGIPMRDMVS
                     ACAAGKVADTIVLDINNEEDQEGQADMPVAYMPNLGKVTLVQLDGVLTPQEYEKCINT
                     GINGCKLVYEFQKKALREKFFGDSTQ"
     gene            12892..13701
                     /gene="rrp42"
                     /locus_tag="VGA92_05805"
     CDS             12892..13701
                     /gene="rrp42"
                     /locus_tag="VGA92_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HEX9845964.1"
                     /translation="MNVTIIDQLKKKKILSLLKDGQRVDGRALDESRPLVIDTGVIPK
                     AEGSARVRLGDTEVVTGVKIQPDKPFPDLGDRGIFICTAEILPLSDPNAEPGPPNEEV
                     IELARVVDRGIRESSMIDLTKLVLQKDKSVIGLFVDNSVTDYDGNLFDACSYASVASL
                     LTCRVPKYELKNDVPTLVEGEFSEPPITTIPVSVTMGKIGDQIIVDPNADEWGCMDAR
                     ITITTNSDGNICAVQKGGNDGFTVEQLVKCSQLSISAAAKIRELLKQAGRK"
     gene            13702..13914
                     /locus_tag="VGA92_05810"
     CDS             13702..13914
                     /locus_tag="VGA92_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37"
                     /protein_id="HEX9845965.1"
                     /translation="MLKKGASLVGLGQKYGIKHRKKFSQVHSILKTKRKCPECGSRQF
                     GRQAVGIWRCKKCGLKIAGTAYDVNL"
     gene            13928..14146
                     /locus_tag="VGA92_05815"
     CDS             13928..14146
                     /locus_tag="VGA92_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HEX9845966.1"
                     /translation="MNAKDKTKAIFDSINVDNKFYPENPTKTQVSLKKNHINISIQAA
                     ELSQMRANINSTLRLIQASSDSVESLKI"
     gene            14170..14571
                     /locus_tag="VGA92_05820"
     CDS             14170..14571
                     /locus_tag="VGA92_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HEX9845967.1"
                     /translation="MSSGQQIPPWLQEQLMKLQQSQQNLQSIMAQKQQLDVEQMEADR
                     ALEELKKAADTDPVYKHAGSVLIKSTKANLIAELEEKKELANTRVTVLAKQETRVKES
                     IKEQETKINEMIRGTQKPSPQQNFTNASGKS"
     gene            14633..15832
                     /locus_tag="VGA92_05825"
     CDS             14633..15832
                     /locus_tag="VGA92_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9845968.1"
                     /translation="MAFRQNTIQQSLQRTFKFRLYPNQVQIEKLHHTLGACRWTYNKM
                     IEKIHSEGFQSRNDLCYFLTELKESEKWLYSHHSKMLQMVAVQIDGAQKALIRLRKNG
                     HHAGDLRFARFSEYRTFTYNQSGFDIRDGFLHLSKISKIKIIQHQNIPENNVIKQVTI
                     SRSKTGKWHACVIVDVDAILPKISLQKSIGIDVGIKNFAYDSDGFVTPNPLNYSKMLQ
                     PLVRAQRKISRRQKGSKNHKKATRFYQIIHERIKNKRKDFLHKLSTQYAKKYDVVFLE
                     RLQKLNMVKNHKMARNVLDSGWGIFGNMLDYKTIMIGVHARNTTIDCSRCGNMIPKSL
                     AVRIHRCRVCGFVLDRDYNAAINILKKGLDIFGITHQLPQELREVTPVEIISRSRKQE
                     TTSFVEW"
     gene            15862..16545
                     /locus_tag="VGA92_05830"
     CDS             15862..16545
                     /locus_tag="VGA92_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HEX9845969.1"
                     /translation="MNIENLRIVVDERERKSGIPDLLKAVGINLEIKTLPIGDYIVAP
                     ETVVERKSISDLISSIFDGRLFDQCDRLREHFAHPIILMEGNVDELEQIVENPFVFYG
                     ALSSIAIDFKIPIVPTPSAAHTAKLLVSMCSRKEITKGPYLKKIKKSDNLQRQQLSVL
                     CSLPGIGEKLATRMLERFGSPSRMLSASLAELAKVEGLGEARAKKIKQMLEFESKLKK
                     QSNQRTLEL"
     gene            16556..17314
                     /locus_tag="VGA92_05835"
     CDS             16556..17314
                     /locus_tag="VGA92_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9845970.1"
                     /translation="MLVSYQWLNERLTDADIVIVDTRPKVAYMYGHIPNSVSLVVDQL
                     IHVSEHGAHLAPDPQSAAQLLGTIGIDHTKTVVVTGESMDPSIARVAWTLAYLGHENT
                     KILDIGINTWQSLGLAITRSQKKLPQTQFTPKIKSEMRIQADELNGLLGKTTILDART
                     PQEYFGGHIPHSTLCPFTDGVGQDGFLFDKKESLQNLFAQKQIPKDDEVICYCMHGHR
                     ASSLFLQLKIAGFERVRLYDGSFIDWHSRRLGLE"
     gene            complement(17291..17779)
                     /locus_tag="VGA92_05840"
     CDS             complement(17291..17779)
                     /locus_tag="VGA92_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX9845971.1"
                     /translation="MAFRVYDASAFYAGIPFASAEQGYITSLVFEEIQHIKKNQGALD
                     VLLQTNRLKIQDPEPSSIDSVTESAKKTGDFQKLSKADMSAVALCYQLKGQLITDDFA
                     ISNLAKNLHLEVYSIMTKGIRTVGRWNYYCPACKKEFSALINCPICGNKLNRKLLKPK
                     SS"
     gene            complement(17769..18584)
                     /locus_tag="VGA92_05845"
     CDS             complement(17769..18584)
                     /locus_tag="VGA92_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX9845972.1"
                     /translation="MKLSRVAIVSKFGSEVAETAAKKVAKKFLAIKSEVFTVAPVVVD
                     GAKKVEVTDELGGKKLDLVITLGGDGTTLRTFRSLTTETPLLSINVGGNKGILSEITL
                     DKIDHAIADIKANKVWLDKRTRVVASVGGEEFPAALNEIYILRQNLTKTAEFEIKFQN
                     DIVKRKMDGVMISTPSGSTGHSFSLGGPILHESLDVLIITPVAPVRLLPSIVVPDEKI
                     QVICNHDTNIVMDAQVVKTAGFEEPVVIKKHKTQAVFVRFKKQGLRQMSRLGF"
     gene            18676..19521
                     /locus_tag="VGA92_05850"
     CDS             18676..19521
                     /locus_tag="VGA92_05850"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9845973.1"
                     /translation="MSYAYPEVLVDTEWASKNPPNDSLRIVEVDYDPENGYRKGHING
                     ASLIWWKRDINDPITRDIIDKKQFETLMSKNGITADSQVMLYGDFNNWFAAFVFWVFK
                     YYGHKNVKIMNGGRKKWELEKKPYTTDEPKISSTTYVAQPPDEGLRAYLFDVKRAIDK
                     NDTALVDVRSPKEFTGEITAPPEYPMEHAQRGGHIPRAQNIPWATAVNDADGTFKSVQ
                     ELQQNYVPKGITPDKDIICYCRIGERSSHSWFVLKYLLGYPQVRNYDGSWTEWGNMIG
                     NPVEK"
     gene            19523..19834
                     /locus_tag="VGA92_05855"
     CDS             19523..19834
                     /locus_tag="VGA92_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845974.1"
                     /translation="MSTELPVLKKGSFYMIKDGPDELIMEDRTKRGLTVQERTIDENL
                     QVFADKGMIHDMDGIGHWVPIRWYFPKKDYGLQNVLVHAEKMEKKYTELRELTCPDDP
                     E"
     gene            19872..20828
                     /locus_tag="VGA92_05860"
     CDS             19872..20828
                     /locus_tag="VGA92_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9845975.1"
                     /translation="MSLLLKDRVYTMESSTAKRGVYPLHGYKLGLYRLPIKLEEPAEM
                     KSIVEGLKKTFTMDTFADRVYATYTWTEESMSDPEAKGYEKVNLPITVEIVTGEVVDL
                     IYQIFPIEKFGDPQWVKDYRKKADYYAKMIIDTILRNTILADKLIEYYVKTEKMSQDA
                     AIERLEELTPLAKIVPDAKPKPKAEVPQEAASVQDVGPALDGPKPGPIDVEYKTHMPP
                     TAQYVVPSNKNAIKTWGRKSGDNSKLGVWGEFVSVDFDICVADGACIDACPVNVYEWF
                     ETPGHPASEKKPLMAREPDCIFCLACEGVCPPQAIKIFQTKS"
     gene            20864..22879
                     /locus_tag="VGA92_05865"
     CDS             20864..22879
                     /locus_tag="VGA92_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEX9845976.1"
                     /translation="MAVNPFTVFIFDIFIVSAILLTAYTFNFYYLAILSIRRKDKHQI
                     ISLGTPSITIQLPIYNERYVATRLVKSVCEMDYPKDKMKIMVLDDSDDDTVEILSDLV
                     TEYKKKGFDIAHIRRGTRKGYKAGALKFAMNLTDTEYVAIFDADFIPPSWFLKKAIPY
                     FTKPEIGLVQCRWGHVNENYSAITQAQALSLDFHFLIEQKAKSNSHLFMNFNGTAGIW
                     RTECIENAGGWHTSTLVEDLDLSYRAQMKGWKCLFISDIVVEAELPVQMNAAKRQQFR
                     WAKGAIQCAIKLLGDILIKRKIPFETKIQAFVQLTRHFAYPLLLIQFLALPILLASNV
                     NLYIVKYVPAITIATYFAMGPAAYLLVIYNLWGKDWKQKAKVLPFLLIYSAGMAVNNT
                     IAVFDALFGKKNEFLRTPKYGIINKSDDWRNKAYNLPFTKTTLLELFFGVYGIIGILV
                     AIFSKNAMFAPIIAIPTIGFCYVAYLSLSHSKSKRIKSTRQRSKTEKMADNYYKLAVI
                     GMFAIILFGGYMAFNGYYKDVYPLDLSRGLLDRIAGGNEPLLMIDDLRKIKTLLPQDG
                     NPVWIFPTDTTNFERLQKDVDSMIKSVESIKNEPANSEEFNTAMFDIHFRAIQLRLNL
                     MDATPYMYASFSNIVFTSIWIAAILGVFTMLKRKKEHLKSYDKSEDV"
     gene            complement(22894..24285)
                     /locus_tag="VGA92_05870"
     CDS             complement(22894..24285)
                     /locus_tag="VGA92_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX9845977.1"
                     /translation="MFKSFGNSLNWKLQLIFNAIAVASVVVISVGNYGAASQLTEKLG
                     AAQGAEFLSNLAFQAIVLGVTVNAAVGAFAFVMARRITKPIIKATEIATRISQGDLTM
                     ILEESKSNDEIGRLLNAEKQMVANLKGVMSEVNLAAQSVSSSAQQIAASGTELNSSVQ
                     QIASTVDQISRGSESQAQGLAKSKQVVEELSSTMGELATNAIESVEVTKKVGSLSERG
                     TESAKEAGERMNKIIKVTNDSAQKVRELAQKTTEITTVLDVIKQIADQTNLLALNAAI
                     EAARAGEAGRGFAVVADEVRRLAESSAKSSEEIDTKLKQIQEDAQVVVEDIETSATEV
                     NQGKLVIESSLKSLDEIATQIRGISDNVKRLSDSAQEEVTKVKVVSTNAAEIAAVAEE
                     NASATEEASAAVEQQTAQTQEIAASANQMSELAEQLQKLISKFKLSAENETSPEPVQV
                     EKKAGLLSKLIHN"
     gene            24607..25962
                     /locus_tag="VGA92_05875"
     CDS             24607..25962
                     /locus_tag="VGA92_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEX9845978.1"
                     /translation="MTTKKSAPKPTKEKSPGISLNKRDSSTLVKKVSAVSDSTKTLSK
                     EIKSMAKIFTENHKVLLSMKEMIDTLTTALEQIQKQSKQMNILEEDNQRIYAGLNQVR
                     AQASIITKINDHTNRLQEQVNKITQAQKEAPDPGSIMKIVDDNKNALQNNTHMIIKIA
                     QRIDNVKEDLQSLSSKADKASSVSAELDEIKKKIIAVFEKADKNNLASEISALKASLG
                     GIIAKTETIPNLRGEIQKIESEIGSLIKRADSAAFVGDGLKSVQTDFASFKDNFFSKT
                     NTIDQKISALSEMLKRSDTSSAEFHQKTDKLFQDFLEVKSATTKSSSTTSKEVTGLLK
                     LSEFQSNIRILAESKYGELKDIEKMAEQTAAIVNLFDRLSIESEEKLSLPQEVRQWAI
                     SKMLDSADKWEIRFSDLFSVLVNKLGQELVKENIRLQQVRDIFGIRGVDEIRKELSLP
                     A"
     gene            26183..>26723
                     /locus_tag="VGA92_05880"
     CDS             26183..>26723
                     /locus_tag="VGA92_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845979.1"
                     /translation="MNSQILEKVQASGVCGIKKAELKKLFGSDCEPSLDKLLKEDKVI
                     VDNKGVAHYVWSKENYLSHISQNDPKFKIISKILKNLENSVNQLKSDFTKTPNDGHVS
                     DFQSHFDKTITELSTSLGWVPFSNIRQKVCQTCNISAETFYSLVLPLIESNQSRYEIS
                     TGGQEGIQVRGMLHGYVRRL"
BASE COUNT         8326 a         5609 c         5681 g         7107 t
ORIGIN      
        1 tgactatgtc aaatttctcc taaaagacgc aaaaacaata gttgacattg aggcaaatca
       61 gactgcccag ctaggtcagc tcttaaaaca acacctagag cgtggagctg actattacat
      121 tctcaaatat actggaaggc cgatgacctc aactgaaatt tacgaatcat taaaaaagat
      181 aatagaaaaa aaggcacaaa aaagggtggt attaactcat ggcgcttaag ctcggcgatt
      241 acaagaccga agtgcacaat gattggtgtc caggatgtgg tgatttcggg atagtcaatg
      301 caatccagat ggcactatcc gaaatgcaaa tcccaagaca caaagcagca atattttctg
      361 ggataggatg ttctggaaaa acatcccact ttatcaatgt gtatggtgtg catacactgc
      421 acggtagggt cttgacgttt gctcaaggcg ccaagcttgc aaatccagag atgacaatta
      481 ttgctgcagg tggggatggt gatgggcttg gaattggtgc aggacacttt gttgcagccg
      541 gaagaagaaa tctggatctt acatacatca tattcaacaa tggcgtttat ggtctaacaa
      601 aggggcaggc ctcgccgacc ctaaagcttg gagaaaaaac aaaatccctt ccaagtccaa
      661 acaccaatta caatgtaaac ccaatcggtc ttgcaatcgc aagcggcttt acgtttgttg
      721 caagaagtta tgcctacgat gtaaggcacc taaaggacat gatagttgcc gctgtacgac
      781 acaagggcct tgcctttttg gacgttctgc agccgtgccc tacgtataat gatatcaaca
      841 caagggactg gtattccgga atggacagaa tggacgaggc aacacaaaag cccaagccac
      901 gaatctacaa gcttgaagag tcaaaatatg atccagtcgt ccactatgac gaagaatccg
      961 agctcaacga aaaactaacc caggcactca tcaggtcttt ggaatgggac acaaaaatcc
     1021 caataggaat attttacaaa aacgaaatca taagccaata cgacaggaga ataatggaca
     1081 aaataccgaa ctatttggaa aatccgcctg cgtcacaggt gatttccgaa aaaggcaaag
     1141 cactaacgga tttatcaaag attttggatt ccttatccgt ttaaaatgct gtaggtgcgt
     1201 ataaaacgga tattagctag atacatggaa gggtatgcta taagacaatt ccaacatgga
     1261 ttaaaagcaa atatcacgcc tagattaata gaaactagat ctgtgttcta tgataaacaa
     1321 caacatattt taaataataa tttaacaaca tgttattatg acgaaactag tttggactga
     1381 tgaaaacact aaaagtgttg aaacatacaa ggctagcgta agatctggcc aaacaaaacc
     1441 agttgtgctt tctggaactc ttagagaagt aataaagaga taaaacatgt ccaactggtt
     1501 ggttctttta tcaccaacat tcatcagttg ggttcaaaaa caagatgata tagccttcga
     1561 gtattatgtt aaagcagttg aaggtataag aaaaatagca gaggcatatt cacaagtcga
     1621 gatagcttca tgtggtcagg gatttcttat tgaggtttat aatcaaaata cggtgttaat
     1681 gtgctttatt tgaagaagaa aaacaaatcc atgttgtacg agtaatttag caaaccccca
     1741 aaccacaaat ccaagaatca ctaaaaccag aatctggaag atgtaaccta aaactaagtt
     1801 atttaacctc ggtttccaag tttaccctat aattgaatct gactgaagca aacaagatat
     1861 ttcggaaatc cataatcaag ggttattttg agccccacct cctgaatctt gattttagca
     1921 aatcaaacat caaacatcca accatccctg atgacggcct gatgcagtca agcttgctgc
     1981 atatcttttt tgatattgat acgggctctg actatcctga cggcgatgag tggtttattg
     2041 cggaatttct ttttccatac agcataaaaa ttccggacca tgtcaagggc cctgattact
     2101 ttaccgcgct ttctttgagc gagggaaaaa atttctggca tcatcgtgag ctgcttcgct
     2161 tcaaatatgg aaaatcaaaa aaactcgaag agtcattaga atttttggat gcaaaataca
     2221 aagagcttca ttctttgcta gagccgctag aaaaagacat caaatgatta ctttccaagt
     2281 gttatttaca agcgcaaatc tcttattgtc tgcaaacaac gtgctcagct tccactttct
     2341 tgtctcgaca tcgaatttgt aaattacttt tgaaatctcg tgtggtatgt taacggaatt
     2401 cagctgaaac ggaagcaatt ctattacaaa gtcgagcttt tcaagggcgt tatcaaacca
     2461 gctcctctcg tgtacgtcta gcgtaaatcc tatttttggc atcccgagga aattgatgtc
     2521 tattctcagc gctttgtcat ttccgcggcg cctcttcatc tccttgaaca tgaacttgac
     2581 tttgtcccac ctgcgatact caaaccactt gaaaaattct tcattaaact ccagcgggat
     2641 tgataatctt agcacgctaa caaaatttgg atcagactct tcaagctcgg tctgtaatac
     2701 gcggaacctt tcggcaaaca gctcgtatac tatttcagct tcccatggcg aaaccccaaa
     2761 gtaatccacc gatgcggtaa ggtagccgtc actcaacaac tattctgaac tagaaaattc
     2821 taattaaagt ttcaagagtg tgggagttag attgtgttta ggtatttcca caggtaaagc
     2881 attcctattg tccctatgac aaacagcatg ggcttccatg caaaaacggg aatgtttggc
     2941 gttgtgagct ttattttgta gttgtcaaaa accatatgaa catagttttt gaccttgtcg
     3001 ctccagactt tgtattctac ttggtgcttt tttagcatgg cttcgatctc ataaatgact
     3061 ggggttctct ggcaaaacaa gtgctgcagg taatccctgg agacttctac ttctgcttgg
     3121 attgccacca ttcctttttt taggtcgaat aatcgcaggt gcatctccca tggctttttg
     3181 agctttaatg agaaaccgtc tcctatttgg gattcttgct tgtgctcaaa tttgactttg
     3241 gtaaatccct cgtttgctaa aattttgagc aagtcttcta cgcttttttt aacaatgact
     3301 gttagctgct cgactccctt cgtgtcgata cttatggtgt gttttttgcc atcgtgaaac
     3361 gagattgtct ttgggtatct cgttagaaac tcttgcatca atgttctatc aactgacgtc
     3421 aaaattgcta ttatttaaac gaatgatttt atttttacaa caaacattac tttttttctt
     3481 gaaatccgcg gacataaacg cacagatccg atgagatggc catctcggtg ggacttattt
     3541 ttctggcagt aagtcttgcc ccagaattct ttactattgc aatcggtgtt gactcggagc
     3601 cctcccccat tttgtgattt gcaattgtag ctaggttatc tgcagttgcc tgcagggtaa
     3661 ctttgagtgg gtttccatcc aaatcctttt gagccctcat gtcttggact ggctcaaggc
     3721 ctgcacacgc aatagctaca ccgcttgtgc caactctggt cggcatgagc ctactgtcca
     3781 ctattatcac accaacacta actccgagat ttaggagaat ttttctgcgc agctgttctg
     3841 caatcaggta gggattatca ggatacaaaa tgactgtgcc ttttctgaca tttgatttgt
     3901 ctattcctgc gtttggggca agtacattat ctgaggatgt gaggacaaac cccgcaattc
     3961 ctccaaatat tgaatctgat tcgcgcaaaa tcacctcaga aaacttggag cccagctgat
     4021 atttttttga taagaccaaa gcgtcagtgg acggccttac tttttctaaa tctattagtc
     4081 ggttttgcga gtttgaaatg tatttgcttg atactaccag tacgtcccca ttttctaaaa
     4141 ttacattgcc aagggaacca actatgctct ggtaaagatc aaaactcgtt tgcttttttt
     4201 ccactctaat tggaattaca ctcagagtca tacaacactt tgtgttggtg gtatttttta
     4261 ctcttcgcga caaagctttt aatctaaact ggctccttca tctcctgtga atattacaga
     4321 aaagatcctt gcaagggcat caggaaagtc gcaggtagct ccagacgata ttatatttgc
     4381 caacgtggac aaggcgatga ttcacgatgt atcgggccca ggagtaatca aggtatttga
     4441 aaagctcaaa aaagaaggaa tcagcgtaga taggctctgg gattcatcaa aaatttgggt
     4501 agctgaggat cactttgtcc catctgcaga taaaatctca gcagaaaaca ttgtaaaatt
     4561 atcaaacttc acaaaacagt acggaataga aaaacacttc aagtacggaa tggggcagta
     4621 cggaatttgc catacattgt ctcatgagga ggcattggta cttccaggag aggtgtatat
     4681 tggcggggat tcacacacaa acacaacagg ggcattaggc gcatttgctt gcggccttgg
     4741 tcatacagac gtagcatatg ttttgcttaa tggaaaaatt tggtttaaag ttccagaaac
     4801 tctgtacttt aagataaatg gcaagcttcc agaaaatgtc atggcaaagg atttaattct
     4861 aaaaattatt ggcgatattg gaaccgacgg agcagcatac gctgcgatgc agtttggagg
     4921 caccggggtt tcagatatgt ctgtggaatc aagactgaca cttaccaaca tgactacaga
     4981 ggcgggtgca aaaaacggaa taatagagcc tgatcaaaaa ctctttgact atcttgcaat
     5041 gcgcggagcc aaaaactatt ctccgacata cggtgacaaa gacgcgcagt atgcaaagat
     5101 gtacgagtat gaggcctcgg agctagaacc acttgttgca aagccatact cgccagaaaa
     5161 catcgcaata gtccgtgacg ttgccggagt agagcttgac aaatcataca tcggctcttg
     5221 cactggtgct aaatacgaag acttggaggc agttgcaaaa attctcaaag gaagaaaggt
     5281 caaaataaga acagaggtct tgcctgctgc catatcaata tacaaaagag cgatggaaaa
     5341 cggccttctc aagatattcc tagatgcggg agcgacagtc ggtccaccaa cttgcggagc
     5401 atgttgtggt gcacatatgg gagtgcttgc aaaagacgaa gtctgcatta gcaccacgaa
     5461 tcgaaacttt cctggaagga tggggcacat agagtcaaag acataccttg catcgccact
     5521 tgttgcagca gcatccgcag taactggaaa aattacagac ccgcgtgatc tatgatggca
     5581 ggcaaagtaa tcaagtacga aaaagacaac atcgataccg atgttattct tcccggacaa
     5641 tatctgaaat tacacgatta tgacgaaatt gcaaaacatg cgatggaggg gatggatccg
     5701 aacttccacg caagggtaaa aaaaggcgac tttatcgttg caggaaagaa tttcggctgc
     5761 ggctcgtctc gtgaacacgc gccaatagcg ctaagccatt gcggcataat gggagttctt
     5821 gctctttcat ttgcaagaat attttatcgc aattccgtag atggggcgtt cttgcttcca
     5881 attgagatag atgagcaaac ctaccaaaaa atatctgatg gtgaccaaat cgaggtaaat
     5941 gtggaaaaaa acgagataaa aaacattaca aaaaacgagg tctacaagat gaagccattc
     6001 tcagagatag catcaaaaat aattgccgca ggcgggctct tcaagtacaa gccagactga
     6061 acctcaaaac aatttctaga atttatatcg cgctgacaca aaacttgatc atacatggca
     6121 aaaacgctct ttgaaaaaat ctgggatcag cacacggtta ctgagattgc cggacgcacg
     6181 ctaatctaca ttgacagaca tttggtgcac gaagttacct ctccgcaggc atttgacggg
     6241 ctgagaataa acaaaagaac ggtacgcagg ccagacctta catttgccac catggaccac
     6301 aacgttccta ccaccaatag atcgcttcca atcatagacc aaatttctgc aatacaaata
     6361 caagcacttg aaaaaaactg caaggacttt ggaatagaat tatttgactt gcacagccca
     6421 caccagggaa tagttcacgt aatcgggcct gagcttggaa ttacgcttcc gggctcgaca
     6481 attgtgtgtg gggacagcca tacctcaaca cacggcgcgt ttggagcctt tgcattgggt
     6541 attggtacaa gtgacgtaga acatgtcttg gcgacccaat gccttgtctt agacaagcca
     6601 aagacatttg aaatcaaagt ggagggaaaa agaaaaaaca ctcacacaat cactgcaaaa
     6661 gacatcattt tgtcgataat aaaaaaaata ggaactggtg ggggggcggg aacggtaata
     6721 gagtatcggg gccagacgat atcgggactc tccatggatg aaagaatgac aatatgtaat
     6781 atgtcaattg aggcaggtgc tagagcaggt cttgttgcac cagatgagaa gacctttgac
     6841 tatcttcggg gaaaaaaata tgtcccaaaa aactacgatg aacttgtgga ctactggaga
     6901 aaaaatctca aaaccgatga gggcgctaaa tttgacaaat cctttacact aaacgcatct
     6961 gatattgcac cgcaggtaag ctggggcaca aatcctgcaa tgactgctga tgtcacagaa
     7021 tctattccag atccgcaaga gtttgcaaag ggggacaaga gccaagaaac tgcagctaaa
     7081 aaagcactag agtacatgga cctaaaggcc ggtacgccga ttaccgacat caaaattgac
     7141 cgggtcttta ttgggtcctg cacaaatgca aggctgcagg atctaattga ggcggcaagc
     7201 ataatcaagg gaagaaaggt cgctccaacc gttcgggcaa tggtagtgcc gggatcacaa
     7261 caagtcaaaa gaaaagcaga ggatcttggg ctagataaaa tctttaagga tgctaatttt
     7321 gagtggcgag aatctggatg cagcatgtgt cttggaatga acccagacat cttggcaaga
     7381 ggtgagagat gtgcaagcac ctcgaacaga aactttgagg gacggcaggg cgccggagga
     7441 aggacgcact tggtaagtcc gataatggct gcagcggcag caattactgg acactttgtc
     7501 gatgttcgcg agtgggcata atggaaaaat tcaaccgcgt cataagcatt ggaacgccac
     7561 ttgataggcc aaacgttgac accgatcaaa ttgtaccaaa acaatttctc aaactagtgc
     7621 agagaaccgg atttggcaag tttctctttt atgattggag gtttgagcca gatggacacg
     7681 caaatgaggg ctttgttcta aacgacccca aatacacttc ttcacacatt ttgattgttg
     7741 gagaaaactt tggctgcggc tcaagcagag agcatgccgc atgggcgcta aaggactatg
     7801 gctttgatgt agtaattgca ccgtcgtttg cagacatctt ctacaataac tgttttaaaa
     7861 atggaatttt gccaataaca attagcaaag gaacagtggc cgaacttgca caaacaagtt
     7921 ctgtcattga ggttgatttg gttactcaga ccatcaaata tgataacaag acagtttcat
     7981 tttcaataga tgagggcaga aagaaaacac ttcttgaagg gctagatgac atcgcagtaa
     8041 cacttttaca ccaagacaaa atcgacgctt ttgaaaaagc tcacgcgctg tgactcattt
     8101 ggctaattat ttttttgaca gtgtcggttt ttcgctcaag aactgttggt gactctacat
     8161 ccatccacag gatttttcct atgtcaaatg agctaactag tccctttttt gaaagcccct
     8221 gcaggacatc ataggacaga tttagatccc tttttgattt tgatttattt atgaggccta
     8281 gtattttgcc actcaaaata tagattccag tgcactcgca atctggtagt cttactagcg
     8341 gcttttcaat aaactccatt accttgcttt ttctgacctt tgcaaaaccg gtctcttctt
     8401 ttctaaactg ccttgtagcg atacaagcaa tactgttttg tcggacaaag ttttggtgca
     8461 tttttttcaa gttaattgcg cccagattat ctacaaacca caacacaaac ggctcatttt
     8521 ttactatgtt tcgtaaatgt agcaaatcgc cggcagttcc acttgcggaa tcctgcaaaa
     8581 acgtgatttt tttggggccc atttttgcaa aatagtgttt tatctgtcct ccaaggcctg
     8641 aaatgtctgc aactatgacg atttcagata tgatcttaaa agaagacaga tagtctacaa
     8701 cgtggctaat tagcggcttc ccatctactg gaatcatggc ttttggaaaa taatcagtaa
     8761 atggctttgc gcgagttcct ctaccacctg ctaagattac tgctttcaat tatcggtatg
     8821 acttttgctt tcttcttctt gcgccagggc caccaaactt ctttggttcc tttcttctgg
     8881 cgtcaccgct tagaaggtgt ttatcaaaat cgtttattct ttttcgcaaa tcggtccggg
     8941 cttgttttgg tagtgggtga tcctttggat cctttttgga cttggtccat ccagtcaaag
     9001 cccttgaaat cgcagttgca gctgcatacg cctggcccat aaagcccccg cctttgacgc
     9061 gaaccgaaat gtcgactttt tttctcaaat cgccggcaat ctcaaggggg ctgagaatta
     9121 cttcgcgtgc agtctcctgt cctatcatct ctgcaggggt gttgtttact ctgaccctac
     9181 cagaaccctt tgtgatgtag acgtgcgcct ttgacgtctt tcttgttgca aaataaatct
     9241 ctgtctttgg gatcattgcc agttgcctac ctctcttgaa agttctgcca tggtagtgta
     9301 gttagcagat gatctggtaa tctttgcctt atccaaaatt attttctttg atgatctaaa
     9361 ctggcttggg acgccaatgt gtgtgcgcag gcgtttgaga gctgcttggc ctgatggttt
     9421 ttttcttgga agcatgccac ggaccattct ttcaatcatt gtgtctggcc ttcttgggtg
     9481 gtacgggcca tgttctggat gtagaatgct ggcgacttcc aaaaaggcct tgtaattttt
     9541 aacgatattg cttttgcttc cgctaatcat tattttttca caatttacaa ctattacacg
     9601 gtttcctttt agcagcattt ttgctacgtt tgagcttagt ctgcctgcaa ggtgatctgt
     9661 tccatctact acgatcaact gagcattatc caataatttt cacgcctttt cctgttggga
     9721 atttggaaat catttcggaa tgattgatta ttttgccgcc agcagttgta attttttttg
     9781 cagcgctagt tgaaattgaa aatgagcaaa gcgtgatttt atgagaaata tctccagtgc
     9841 ccaaaacctt gcctgggaca acaattacgt cattatcgcc agttacatcg tttagcttgc
     9901 cgacatttac tattcgtcgc gcagcccctg gcttttgggc catttgagca agccttgacc
     9961 agattggcgc cttgtttttg gacgaagcta tcttgagctc ctttactagg ccgataacta
    10021 actgattggt cataattaat gccctttaac aacccaaata tattattatg attttagctc
    10081 ggcaactact tgcctgaact cttctagtct tccagcaagt tcatctatgc cagttgtgat
    10141 gatttgttct gggcttagcg cgcctgtact ttctacagtt agaacgtact catcttgctt
    10201 gtctgtatgg gttagaacgg aaatgtttgc agaattccac tttgcatgat ccgatccacg
    10261 accaagtctt gcatatgctt ctagctttag tttttgaccc ggcgcaactt gtacaattgg
    10321 aatcttgtct gagactggct ttacagtctc gtcttctgaa ctaaattcag ctgatgtgac
    10381 agtccttgtc gtatccgtat ccccagaatc gattactagc attacgcgac aattagaaca
    10441 gccagtcttg ctttggcagg gacacttggc cggctcattg aagcgcttta ggtctgtctt
    10501 tattggtatt aggccaagtc tgtgggcaac tacctcgtct gacattactg aagagttttc
    10561 aatgacgtct actgtgtcta tggcaaagac tggaacgccg tttaggcaaa ccctccttag
    10621 cgcatttgca tactgcaagg gaactccttt cagcttgact gacatgcgct gtttgtcttg
    10681 tgaaattatc tccaaagaag gcaaatctta gaaaaatttt tccaaatcca cataaaaatc
    10741 tagcgagttt tctgacatga gataaatact tagatttcgt tatcaaatcc atgacagacg
    10801 ttacggtaat taggcattcg gttgcggggg aaaaattcga gattctagtc aaacctgacc
    10861 cggcactgga atacaagatg ggcaaaagaa aagatgtctc ttcagtcctt gtttcagacg
    10921 aagtatacac ggactctaat aagggaacga gggcatcaac agaaaaactg ctcaaggcct
    10981 tcaataccca ggacacaaat gtgataattg aacagattct caaaaaaggg gatcttaacc
    11041 taactactga tcagcgcaga aaaatgactt ccgaaaaaag aaaacaaatc attaactatg
    11101 tcgcaaaaac atttgttgat ccgagatcac acctgccaca cccaccacta agaatagagc
    11161 aagccttaga gcaagcccgt atctcaatag atccattcaa aaacactgag gagcaagcaa
    11221 gggatgttgt tgaaaaactg cgtacaataa ttccactaaa gtcagaaaac atgctacttg
    11281 aaattacagt ccctgcgcaa tatgcagcac aatcatattc tgttttaaaa tccaacggca
    11341 ctctaaaaaa agaagaatgg caaaacaacg gatcacttaa agcaatacta gaaatacctg
    11401 ctggggcaag ggcaaacgtg attgacagac tcggctccat aacaaagggc agtgctaccg
    11461 tggagatggt caagtaatgg atgatataca acgaaaatac gtcatacctg gagacgtaat
    11521 aacgactggg ccgtaccggg ctgaagaaaa cgttcatctt tttggggata aaattattgc
    11581 aacgactgtg ggaatttctg aaatttatga cagcggagtt agagtcattc cgcttaccgg
    11641 catttatgtg ccgcgaattg atgatttcat tattggtatg gtaaaatcac acacctctct
    11701 ttcttgggag cttgatatca acgcgtgtta tcctggaata ctgcctgccc aagatgtctt
    11761 tgggagggac tttaatccca aagtggacga gctgacatcg cgactaaaga ggggggatct
    11821 gattgcagcc cgtgttgcaa attccgacag atccagagac ccgctaatta caatagctga
    11881 tagggacttg ggcaaaatag atgatggcga gctaatcaag atttcaccaa caaaggttcc
    11941 gcgactaatt ggaaaacgcg gctctatgat tcaaacaata gaaggtgcca caaaggcgca
    12001 aattacaatt ggacaaaacg gatacgtcgt cgtttcttgc gaggaagccg agggattatt
    12061 aaaggccctt gacgccataa aaacagtcga agaacaagca cacgttccaa atttgactga
    12121 aaaaatacaa gaaatgttag gatcaaacag tgaataaaaa atgggtgtaa ggaaaacaga
    12181 tatcgtatta ctaagtgata aaggaatcag atgcgatgga agaagcatcg acgagccacg
    12241 cagaattatg atcaaagcgg gcgttctaaa gaatgcaaac ggttctgcat acattgaatt
    12301 tggcgaaaac aaaatcctag ctggcgtttt tggcccacgc gacgtccacc caaaacactt
    12361 ggcaaatacc gacaggggaa tcctgcgctg cagataccac atgcagccat tttccgttgg
    12421 cgaaagaaaa aacccagccc catcaagaag ggagattgag atttccaaag taatcaagga
    12481 ggccctagag ccagcagtaa tgcttgagaa ttttccaaga actgtagttg acgtctttat
    12541 tgaagttctc caagcagatg gcggctccag atgtgcggcc ttggatgctg cagcagttgc
    12601 cttagctgac gccgggattc cgatgaggga tatggtttct gcatgtgcgg caggcaaggt
    12661 tgccgatacc atagttttag atatcaataa tgaagaagat caggaaggac aggctgacat
    12721 gccagttgca tacatgccaa acctgggcaa agtaacacta gtccagcttg acggggtctt
    12781 gactcctcag gaatacgaaa aatgcatcaa caccggaatc aacggatgca agcttgtcta
    12841 tgaattccaa aagaaggcgc tacgagaaaa attttttgga gatagcacac aatgaacgtt
    12901 acaataattg atcaactaaa aaagaaaaaa attctttcac tcctcaaaga cggtcaaagg
    12961 gtagatggaa gggcactaga cgagtcgcgt ccgcttgtta ttgacacagg agtaatccca
    13021 aaggcagagg gctctgctag agtaaggctt ggagataccg aagtagtaac tggagtcaag
    13081 attcaaccag acaaaccgtt tcctgattta ggtgataggg gcattttcat ctgtactgcg
    13141 gagatcctgc cactttctga tcctaacgcg gagccgggcc caccaaacga agaggtaatt
    13201 gagttggcac gtgtagtcga caggggaata cgggaaagca gcatgattga tctgacaaaa
    13261 cttgtattgc aaaaagacaa gtctgtaatc ggtctttttg tcgacaacag cgtaactgat
    13321 tatgatggaa atctttttga tgcgtgttct tatgcctctg ttgcaagtct cttgacatgt
    13381 agggtgccaa aatacgagct aaaaaacgac gtgccaacct tagtggaagg agagttctca
    13441 gaaccgccaa tcactacaat tcctgtatct gtaacaatgg gaaaaatagg cgaccaaatc
    13501 atcgttgatc ctaatgcgga cgaatggggt tgtatggatg caagaataac tattactaca
    13561 aactctgatg gaaacatctg cgcagttcaa aagggtggaa atgacggatt taccgttgaa
    13621 cagttggtaa aatgctcaca actctcaatt tcggccgcag caaaaataag ggagctccta
    13681 aaacaggcag gtagaaagta aatgctcaaa aaaggtgcat cattagttgg tcttggacaa
    13741 aaatacggaa taaagcacag aaaaaagttt tctcaagtcc attcaatcct aaagacaaaa
    13801 agaaaatgtc cagagtgtgg ctcacgtcag ttcggaaggc aagcagtagg aatctggcga
    13861 tgcaaaaaat gtggattaaa gattgctgga acggcctacg atgttaacct ttagcgctaa
    13921 catttcagtg aatgcaaaag acaagacaaa agcaattttt gactctataa acgtggataa
    13981 caagttttat ccagaaaacc caactaagac tcaagtctct ttgaagaaaa atcacatcaa
    14041 catatcaatc caagctgctg agctttccca gatgcgtgca aacatcaact ctactttacg
    14101 actaatacag gcaagctccg attcagtcga atcgttaaag atataagaaa agctggcaag
    14161 ctgttctgta tgtcttctgg gcaacaaatt cctccatggc ttcaagagca gctaatgaag
    14221 ctgcaacagt cccagcaaaa ccttcaatca atcatggctc aaaaacagca acttgatgta
    14281 gaacagatgg aggcggacag ggcactagaa gaactaaaaa aagcagcaga caccgatcct
    14341 gtctacaaac acgccggttc cgtcttgata aaatcaacaa aggcaaacct tattgcagaa
    14401 cttgaggaaa aaaaggagct tgcaaatacg agggtaacgg ttttggcaaa acaggaaaca
    14461 cgagtcaaag aaagtatcaa ggaacaggaa acaaaaataa atgaaatgat acgtggcacg
    14521 caaaaaccca gcccacaaca aaattttaca aatgcgagcg ggaaatccta aaccctttag
    14581 ggccgggatg aaagcgagct gttgacagca agtaaatacc aagactacca aaatggcttt
    14641 taggcaaaac accatacaac aatcgttgca aagaaccttc aagtttcgtc tatacccaaa
    14701 tcaagtacag atagaaaaac tgcaccacac acttggcgct tgcaggtgga cctacaacaa
    14761 aatgatcgaa aaaattcatt ccgagggatt tcagagccga aacgacctgt gttattttct
    14821 gacggaatta aaagaatcgg aaaagtggct gtattcacac cactccaaga tgctacagat
    14881 ggtggcagtg cagattgacg gcgcacaaaa ggcattgata cgactacgaa aaaacggaca
    14941 ccatgccggt gatctgaggt ttgccagatt ctcagaatac cgcaccttca catacaacca
    15001 gtctggattt gacatcaggg acggcttttt acatctctca aaaattagca aaatcaaaat
    15061 aatacaacac caaaacattc cagaaaataa tgtcatcaaa caagtcacaa tatcaagatc
    15121 aaagacggga aaatggcacg cgtgtgtgat tgtagatgtg gatgcgatat taccaaaaat
    15181 atcacttcaa aaatcaattg gaatagatgt cggcatcaaa aatttcgcat atgattctga
    15241 cggatttgtg actccaaacc ccctgaacta ttccaaaatg ctacagccat tggtaagagc
    15301 ccagagaaaa atatcacgaa ggcaaaaggg ttcaaaaaat cacaaaaaag caaccagatt
    15361 ctaccagata atccatgaaa gaatcaaaaa caaaagaaag gattttctgc ataaactctc
    15421 aacacaatat gcaaaaaagt acgatgtggt atttttagaa aggctgcaaa agctcaacat
    15481 ggtaaagaat cacaaaatgg cacgaaacgt tctggattct ggatggggca tatttggaaa
    15541 tatgctggac tataagacaa taatgatcgg tgttcatgca agaaatacta caatagattg
    15601 ttcgagatgt ggtaacatga taccaaaatc cttagctgtt agaattcaca gatgtcgtgt
    15661 gtgtggtttt gttctggaca gagactacaa tgcagcaatc aacattttga aaaaaggatt
    15721 agatattttt ggaattacac atcaactacc gcaggaattg cgggaagtta cgcctgtgga
    15781 gatcatatcg agatcaagga agcaggaaac cacgagcttt gtcgagtggt agttcaccaa
    15841 tcaaagcaga tcctaaatcg gatgaatatt gagaatctcc gcatagttgt tgatgaaagg
    15901 gagagaaaaa gcggcatacc agacctgctc aaagccgtcg gtatcaactt ggaaataaag
    15961 acactcccaa tcggggatta tattgtcgca ccagaaaccg ttgtggagcg aaaaagcatc
    16021 tctgacctaa tctcttcaat atttgacggc aggttatttg accagtgcga cagactgcga
    16081 gagcactttg cgcacccaat tattcttatg gagggaaatg tagacgaact cgagcaaata
    16141 gtggaaaacc catttgtgtt ctatggggcg ctttcttcca ttgcaattga ttttaaaatt
    16201 cccatagttc caacgccaag tgctgcccac acagcaaaac tccttgtttc aatgtgctca
    16261 cgaaaagaaa taacaaaagg cccgtacctt aaaaaaataa aaaaatcaga taacctgcaa
    16321 aggcagcaac tttccgtttt gtgcagcctt ccgggaattg gagaaaaact tgcaacacgg
    16381 atgcttgagc gatttggctc tccgtcacgc atgctcagcg cctcacttgc agagcttgca
    16441 aaagtggaag gacttggaga ggcacgcgcc aaaaaaataa aacaaatgct tgaatttgaa
    16501 agcaagctaa aaaaacaatc aaatcaaaga acacttgagc tgtagctgag taatcatgtt
    16561 agtttcttac cagtggttaa atgaacgcct caccgacgca gatattgtaa ttgtggatac
    16621 gcgacccaaa gtagcataca tgtatggaca cattccaaac tctgtttcgc ttgtagttga
    16681 tcagctaatc cacgtgagtg agcatggcgc acatcttgcc cccgacccgc aaagcgccgc
    16741 tcagcttctc ggcacaattg gaatagacca tacaaaaact gtagtcgtga ccggggaatc
    16801 aatggatcct tcgattgcgc gagttgcgtg gacgcttgcg tatcttggcc atgaaaacac
    16861 caagattctt gatattggaa taaacacatg gcaaagcctt ggtcttgcca tcacaagatc
    16921 acaaaaaaag ctacctcaaa cacaatttac cccaaaaata aaatcggaaa tgcgaataca
    16981 agccgacgag ctaaatggct tgctgggcaa gacaacaata ctggacgcac gaaccccaca
    17041 ggaatatttt ggtggacaca tacctcactc gacgctttgc ccctttactg acggcgtggg
    17101 gcaagacgga tttttgtttg acaaaaaaga atcacttcaa aatctttttg ctcaaaaaca
    17161 aatccccaaa gatgatgagg tgatatgtta ttgtatgcac ggccataggg cctcaagtct
    17221 ttttttgcag ctaaagattg ctggctttga gagagtcagg ctatacgatg gctcgtttat
    17281 tgactggcat tcacgaagac ttgggcttga gtagttttcg gtttagctta tttccacaaa
    17341 ttgggcagtt tatcagagcc gagaattctt ttttgcaggc agggcagtaa taattccacc
    17401 ttccaacggt ccttattccc tttgtcataa tagaatatac ctcaagatgc aagttttttg
    17461 caagatttga tattgcaaag tcgtctgtta ttagctggcc ttttagctgg taacacaagg
    17521 caactgcaga catgtctgcc tttgataatt tttgaaagtc gcctgtcttt tttgcagatt
    17581 cagttacaga atcgatactg gatggctcgg ggtcttgtat ctttagtcgg tttgtttgaa
    17641 gcaacacatc aagtgcaccc tggttttttt tgatgtgctg tatctcttca aaaacaagcg
    17701 acgtgatgta cccttgctca gctgatgcaa agggaattcc cgcataaaac gcgcttgcgt
    17761 cataaaccct aaaagccaag cctgctcatc tgtcgcagtc cctgtttttt gaatctcaca
    17821 aagactgctt gggttttgtg ttttttgatc acaactggtt cttcaaagcc ggctgtcttg
    17881 actacctgtg catccatgac aatgttcgtg tcatgattgc aaatcacctg aatcttttcg
    17941 tctggcacca caattgacgg tagtagtcga actggagcaa ctggggtaat tatcaaaaca
    18001 tctaggcttt catgaagtat tgggccgcca agggagaaag agtgaccagt cgaaccgctt
    18061 ggcgttgaaa tcataacgcc gtccatcttg cgttttacta tatcattttg gaatttgatc
    18121 tcaaactcgg ctgtctttgt gaggttttgt ctgagaatgt aaatctcatt tagcgctgcc
    18181 gggaactcct ctccgcctac ggaggcaaca acgcgagttc ttttatcaag ccagaccttg
    18241 tttgccttaa tgtcagctat ggcgtgatca attttgtcta gcgttatctc ggagaggatg
    18301 cctttgtttc ccccaacatt gatggacaat agcggagtct cagttgtcag gctcctaaaa
    18361 gttcgcagtg ttgtaccgtc gccaccaagc gtgatgacca agtctaattt tttaccaccg
    18421 agctcgtcgg taacttctac tttttttgca ccatctacta ctactggagc aactgtgaat
    18481 acctcggact tgatggcaag aaactttttt gcaacctttt ttgctgcagt ttcagctact
    18541 tcagaaccaa acttgcttac tatggcaacg cgacttagct tcaatcaacc actctttcta
    18601 tggttttgtt taaaaaacat gtctttgtca cacacaaaaa caattaagta ggaaatcaaa
    18661 tccctgtaga ttggtatgag ttacgcttac cctgaagttt tagttgatac tgaatgggca
    18721 tcaaagaatc ccccaaacga tagtctcaga attgttgaag ttgactatga ccctgaaaac
    18781 ggatacagaa agggtcacat caacggagca agtctgattt ggtggaagcg agacataaac
    18841 gacccaatca ctcgtgacat aatagacaaa aagcaatttg agactttgat gtcaaaaaac
    18901 ggcattacag cagacagcca agtgatgctc tacggtgact ttaacaactg gtttgccgcg
    18961 tttgtattct gggtcttcaa gtattacgga cacaaaaatg taaagataat gaatggtgga
    19021 agaaaaaaat gggagctcga aaaaaaacca tacacaacag acgagccaaa gatctcctca
    19081 actacatatg ttgcacagcc acccgatgag ggattaaggg cgtatctttt tgatgttaaa
    19141 cgtgcaatag acaaaaatga cactgcgcta gtggatgtgc ggtctccaaa ggaattcaca
    19201 ggagaaatta cagccccacc ggaatatccg atggagcatg ctcaaagagg cggtcacatt
    19261 cctagggcgc aaaacattcc gtgggccacg gcagtaaatg acgctgatgg aactttcaaa
    19321 tctgtccagg aattgcaaca aaactatgtg ccaaagggca taactccaga caaggacata
    19381 atctgttatt gcaggattgg tgaaagatca tcacattcgt ggtttgtact caagtacctg
    19441 ctcggatatc cacaggttag aaactatgat ggttcttgga ccgaatgggg caacatgatt
    19501 gggaaccccg tagaaaaata agttgagcac agaactccca gttctcaaaa aaggctcgtt
    19561 ttacatgata aaggatgggc cagacgagct aatcatggaa gataggacaa aacgtggtct
    19621 cactgtacaa gaacgcacaa ttgatgaaaa cctgcaggtt tttgccgaca agggaatgat
    19681 ccacgacatg gatggaatag gacactgggt tccaattcgt tggtattttc caaaaaaaga
    19741 ctatggtctg caaaacgttc tagtgcacgc agaaaaaatg gagaaaaaat acaccgagct
    19801 gcgtgagcta acctgtcctg acgatccgga atagataaga tttttatctc atctacgaga
    19861 aattagagca tatgtcactt ctcttaaaag atcgagtata caccatggaa tcctcaactg
    19921 cgaaaagggg cgtttatcca ctacatggct acaaacttgg tctttatcga ctcccaatca
    19981 aactagagga gccagcagag atgaaatcaa ttgtggaggg actcaaaaag acattcacga
    20041 tggatacatt tgctgaccga gtttatgcga catatacttg gactgaggag agcatgtctg
    20101 atcctgaggc aaaaggatac gaaaaagtca accttccaat cacagtagaa attgtaacag
    20161 gcgaagtagt tgatttgatt taccaaatat tcccaattga aaagtttggt gacccacaat
    20221 gggtaaagga ttatcgtaaa aaagcagatt attacgcaaa gatgataatt gataccattc
    20281 tacgaaacac cattttggca gacaagctga tagaatacta tgtcaagaca gaaaaaatga
    20341 gccaagacgc agcaatcgaa agactagagg agctcacccc acttgcaaaa attgtaccgg
    20401 atgcaaaacc aaaaccaaag gccgaagtac cgcaagaggc agcctcagta caagacgtag
    20461 gtccagcgct tgatggtcca aagccggggc caattgatgt ggaatacaaa actcacatgc
    20521 ctccaactgc acaatatgtc gttccatcaa acaagaatgc cataaagaca tgggggagaa
    20581 aatcaggcga taactcaaaa ttgggtgttt ggggcgaatt tgtatcagtt gattttgata
    20641 tttgtgtagc agacggcgcc tgcattgatg cgtgccccgt taatgtctac gaatggtttg
    20701 agacgcctgg ccacccggca tctgagaaaa aaccgttgat ggcgcgtgaa cccgactgca
    20761 tattttgctt ggcatgtgaa ggggtatgtc caccacaagc aatcaagatc ttccagacaa
    20821 aatcctaaga gtcataaagt cgaccaataa aggctgagat gtaatggcag tcaatccttt
    20881 taccgttttc atatttgata ttttcattgt ttctgcaatc ttgctgactg catacacctt
    20941 caacttctat tacttggcaa tcctctcaat taggagaaaa gacaaacacc aaatcatttc
    21001 gcttgggacg ccttcgatta ccattcagct cccaatttac aatgagcggt atgtagccac
    21061 aaggctagtc aagtcagtgt gcgaaatgga ctatccaaaa gacaaaatga aaatcatggt
    21121 tcttgatgat tcggacgacg atactgtgga aattctaagt gatcttgtga ctgaatacaa
    21181 gaaaaaggga tttgatattg cgcacataag gcgtgggaca aggaagggct acaaggcagg
    21241 agcactcaaa ttcgcaatga atctgaccga tacagaatat gtggcaatct ttgatgcgga
    21301 ctttattccg ccgtcgtggt ttttgaaaaa agcaattcca tattttacaa aaccagaaat
    21361 aggtcttgta cagtgcaggt ggggccatgt taacgaaaat tattcagcaa tcacacaagc
    21421 acaagcacta agccttgact ttcactttct gattgaacaa aaagcaaaaa gcaactctca
    21481 cctttttatg aactttaatg gaactgcggg aatctggaga acagaatgca ttgagaacgc
    21541 tggtggctgg cacacatcta ccctagttga ggacttggat cttagctaca gggcccaaat
    21601 gaagggctgg aaatgccttt tcatttcaga cattgtagtt gaagcagagc ttccagtaca
    21661 gatgaatgct gcaaaaaggc agcagtttag gtgggcaaag ggcgcaatcc aatgcgcaat
    21721 aaagcttctt ggagacattt tgataaaaag aaagattcca tttgaaacca aaatccaggc
    21781 tttcgtgcaa ctaacgcgac attttgcata cccacttttg ttaatccagt ttcttgcact
    21841 gccgatactt ttggcttcta acgttaatct gtatattgtc aaatatgttc cggcaattac
    21901 cattgctaca tattttgcaa tgggtcctgc cgcatacctt cttgtaattt acaatctctg
    21961 gggcaaagac tggaaacaaa aagcaaaggt tctgcccttc ttgctcattt attcggcagg
    22021 aatggcagta aacaatacga ttgcagtctt tgatgcattg tttggcaaaa aaaacgagtt
    22081 tttgcgaact ccaaagtatg gaatcataaa caaatccgat gactggcgca ataaagcata
    22141 caacctgccg tttacaaaga ctacgctttt ggagctgttc tttggagtct atggcataat
    22201 aggcatacta gttgcaatat tttcaaagaa cgccatgttt gctccaatca tagcgatacc
    22261 tacgattgga ttctgttatg tggcatacct tagcctctca cactctaaat ctaaaagaat
    22321 taaatcaacc cgccaaagat caaagacaga aaagatggca gacaattatt acaaactggc
    22381 agtaatcggc atgtttgcca tcattttgtt tgggggatat atggccttta acggatacta
    22441 caaggacgta tacccgcttg atctctcacg aggactcctc gatagaattg ctggaggaaa
    22501 cgaaccgtta ttgatgattg atgacctgcg caaaataaag acactgcttc cacaagatgg
    22561 caatccagtc tggatatttc caacagatac aacaaacttt gagcggctgc aaaaagacgt
    22621 ggactctatg attaaaagtg tcgagtcgat aaaaaacgag ccagccaaca gcgaggaatt
    22681 caatacagca atgtttgata tccactttag agcgattcaa ttaagactaa acctgatgga
    22741 tgcgacacca tacatgtatg caagcttttc aaacatcgta tttacctcaa tttggattgc
    22801 tgcaatactg ggagtcttta ccatgctaaa gaggaaaaaa gaacacctca aatcctacga
    22861 caaatccgaa gacgtttaga attctgaaaa atcctaatta tgaattagtt ttgagagaag
    22921 gcctgctttc ttttccactt gtactggttc tgggcttgtt tcgttttcag cacttagctt
    22981 gaactttgaa attaattttt gtagctgctc tgcaagctct gacatctggt ttgctgaagc
    23041 cgcaatctct tgtgtttgag cggtttgttg ctctactgct gctgaagcct cttctgtagc
    23101 tgatgcgttt tcttctgcta ctgcggcaat ttctgctgcg tttgtcgaaa ccacctttac
    23161 tttggtgact tcttcttggg cagaatctga aagtcttttt acattatcag aaattcctct
    23221 aatctgagtg gcaatctcat caagtgattt tagtgaagac tcgattacaa gcttgccttg
    23281 gtttacctca gtggcacttg tttcaatgtc ttctactact acttgcgcat cctcttggat
    23341 ttgctttagt ttagtatcga tttcctcaga tgattttgca gaactttccg ctaggcgtct
    23401 tacctcgtct gctactactg caaaaccacg gcccgcctcg cctgcacgcg cggcctcaat
    23461 tgcggcattt aatgccaaaa gatttgtctg atcggcaatc tgctttatga cgtctagaac
    23521 ggtagtaatt tcggtggttt tttgtgcaag ctctcttacc ttttgcgctg aatcatttgt
    23581 tactttgatt attttgttca ttcgctcgcc tgcctccttt gcggactcgg ttcccctctc
    23641 agataggctg ccaactttct ttgttacctc tacggattca attgcatttg ttgcaagctc
    23701 gcccatcgta cttgataact cctcaacaac ctgctttgat ttggctaagc cctgtgcttg
    23761 tgattctgag cctcttgaga tctgatcaac tgtggatgca atttgctgaa cagacgagtt
    23821 taactctgtt ccagaggcgg caatctgttg tgcgctagat gagacagact gtgcagccag
    23881 attcacctct gacattacgc cttttagatt agcgaccatt tgtttttcgg catttagtaa
    23941 tcttcctatc tcatcgtttg attttgattc ttcgagaatc attgtgaggt caccttggct
    24001 tattctagtc gcaatttctg tagccttgat tatcggcttt gtgattctgc gtgccatgac
    24061 aaacgcaaat gcgccaacgg cggcattaac tgtcacaccc aaaacgatcg cctgaaacgc
    24121 taaatttgat agaaattctg ctccttgggc cgcgccaagt ttttctgtaa gctggctggc
    24181 tgcgccgtaa ttgccaaccg atattacaac aacggaagca actgcaatgg cgttaaagat
    24241 taattgtaat ttccaattca gggaatttcc aaaacttttg aacatattac gtatctttat
    24301 gcacttagat tataggcacg agtgtgttta ggataaacga aatatttctg ataatttact
    24361 cgtaaatgag gctggaaagc tggttattat gatattacct agtttttatt agtacggtat
    24421 ggtacggtac tatcatgatc tcatgtgaat actgcacaaa gcagtttata gagacagcca
    24481 atgggttgac agaaaaaaca ctccacgaaa tacttcatgg gccagaattg gtcaatgagt
    24541 agctacatca ttttttatct ttttttagcc gaatctttta ttattcacaa gacgtcaaca
    24601 taagccgtga ctacaaagaa atctgcacca aagccaacta aagaaaaatc acctggaata
    24661 agtctcaaca agcgagattc tagtactctg gtcaaaaaag tatctgccgt ttctgattca
    24721 acaaaaacat tatcaaaaga gatcaaatct atggcaaaaa tttttacaga aaatcacaag
    24781 gttttgttgt caatgaaaga aatgattgac accctaacta ctgcgcttga acaaattcaa
    24841 aaacaatcaa aacagatgaa catactagaa gaagacaatc agaggatata tgctggatta
    24901 aaccaggtga gggctcaggc ctccataatt acaaaaatta acgatcatac aaatcgacta
    24961 caagaacagg taaacaagat cactcaggcc caaaaagagg cacctgatcc aggcagcata
    25021 atgaagatag tagatgacaa caagaacgcc ctccaaaaca acacacacat gataattaag
    25081 attgcgcaac gaattgacaa cgtaaaagaa gatttacaga gtctgtcatc aaaggccgac
    25141 aaagcctcat cggttagcgc cgaactcgat gagataaaaa agaagatcat agctgtattc
    25201 gaaaaggcag acaaaaacaa tctagcgtca gagatttcgg cactaaaagc aagtcttgga
    25261 ggcataatcg caaagactga gacaataccg aaccttcgag gcgagattca aaaaatagaa
    25321 tccgagattg gctcgctcat aaaaagagct gactctgcgg cgtttgtcgg agatgggctt
    25381 aaatcggtgc aaactgactt tgcgtcattt aaggataatt tctttagcaa gacaaacaca
    25441 atcgaccaaa agatttccgc gctatctgag atgctaaagc gctctgacac atcatctgca
    25501 gaatttcatc aaaagacaga caagctattc caagacttcc ttgaagtcaa aagtgctacg
    25561 accaagtcgt catctactac atcaaaggaa gtaactgggc ttcttaagct atcagaattc
    25621 caatccaata tcagaattct tgcagaatca aaatatggcg agctaaagga cattgaaaaa
    25681 atggcggagc agactgcggc aattgtcaat ctttttgaca ggctgtcaat agagtctgaa
    25741 gaaaagcttt ccctcccaca agaggtgcgc cagtgggcaa taagcaaaat gcttgattcg
    25801 gcagacaagt gggagataag attctctgat ctgtttagcg ttctagtaaa caagcttgga
    25861 caggagctag tcaaagagaa cattcgatta cagcaggtgc gtgatatctt tggaattcgc
    25921 ggcgtcgacg aaataagaaa agagctcagc ttgccagcct agattttttc aagcagtcag
    25981 ataccccagg tctcattcca cattagtttc agatgaccta atcatcattc tgcagactgt
    26041 aattgtttgg cgtactaata attcttgatt ttttggcaca ctctaaacca attttttaga
    26101 tgtagatgag gcaacaatta ttcacaaaag tgtagtacaa gtcgacctcc cctattaaaa
    26161 aaaattttag ggagttgaag ccatgaattc ccagattctt gaaaaagtac aagcgtcagg
    26221 agtctgcgga ataaaaaagg cagagctcaa aaagctcttt ggaagcgact gtgagccatc
    26281 tcttgacaaa ctcctaaaag aagacaaagt catagttgat aacaaaggcg ttgcccacta
    26341 tgtgtggagc aaggagaact atctttcaca catttctcaa aacgatccaa aattcaagat
    26401 tatctcaaaa attctcaaaa atctggaaaa ttcggtaaac cagctaaaaa gcgattttac
    26461 caaaacacca aacgacggcc acgtctctga ctttcaaagt cattttgata agacaataac
    26521 ggagctatct acatcgttag ggtgggttcc attttcaaac atcaggcaaa aagtttgcca
    26581 aacatgcaat atttcagctg agacattcta ttctttggtt ttgccattaa ttgaatcgaa
    26641 ccaatcaaga tatgagattt cgactggagg tcaggagggt atccaagtgc gcggcatgct
    26701 gcacggatac gtaagaaggc tat
//
LOCUS       DASWKH010000080        44895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3524800, whole genome shotgun sequence.
ACCESSION   DASWKH010000080 DASWKH010000000
VERSION     DASWKH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 44895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44895
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524800"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..1623)
                     /locus_tag="VGA92_05885"
     CDS             complement(271..1623)
                     /locus_tag="VGA92_05885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9845980.1"
                     /translation="MTKKRILVLGGGFAGIECTRKLESYFKRDSDIELILISEDNFLL
                     FTPMLPQVASGMIETRHIIIPIRTICKKTTFYEGRVKNIDPYGKYVTLYGTNEKRGIS
                     LEYDYLVVALGSQTNFFGNQSVENHAYTMKSLNDAVVLRNRIIDMLEQAENETDPILR
                     QSLLTFVIVGAGFAGIETAGEMHDFLLDAKKHYPNISEKEIRVIVLEALSIVLPGFSD
                     KLAKFTKDKLIQRGIEIMLNTMVVSFDGSEVLIKDAVSDDKTAVMPAELRSIQTKTLI
                     WTAGVTPVDTIKNSLFKTDRGRVMVNEYLEVPEFPGAFAIGDCSLTINPQTNKPYPPT
                     AQNAEAEAKFAAYNLYATIKGKIKKKIDYMSKGQMAIIGKRTAIAQIYGMNIYGFIAW
                     WIWRTVYLRKVPKFNKRLRIFLDWTADLLFDRDISRLKIIRKEQPIDYKELDEVDDVW
                     "
     gene            1735..2055
                     /locus_tag="VGA92_05890"
     CDS             1735..2055
                     /locus_tag="VGA92_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845981.1"
                     /translation="MKKEYVVVRIDAAPDGAPYVVISLSSAKDFKENNQLPQSPLGSG
                     MMGFSNMDDMMKGLNKMLTGGVFGMPGGVTSMKLDMREYKELGLSVGDRIHLEITKPE
                     NTGV"
     gene            complement(2056..2706)
                     /locus_tag="VGA92_05895"
     CDS             complement(2056..2706)
                     /locus_tag="VGA92_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9845982.1"
                     /translation="MSVPETVREIITRNRSIYDCMKMDVINYTALAVKIQPDVERQIG
                     NPVNLNTIVVAIKRYSDTFVEKDDLKSESILKNARLSLTDGILDIKFSANDIDKNDAA
                     SILSKFEQFDSDYEFFRLADSTFRVLTEDLVDIRKMFESIPSTNNFLNAGLAKIKIRI
                     SQDQNRSDVVSYVAELLHSNGLELQNAFFSQDEIVLVLREGDASKAYEVLRAEISR"
     gene            2826..3329
                     /gene="hsp20"
                     /locus_tag="VGA92_05900"
     CDS             2826..3329
                     /gene="hsp20"
                     /locus_tag="VGA92_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HEX9845983.1"
                     /translation="MTMFFDEEVDRLFKKMSKSFMDFNDIFEDVKRSDGKTFGPYYYG
                     YSITVGPDGKPQIREYGNVKPSLLPSSEVREPLVDTLVDDKENTLKLVAEMPGVEKKD
                     IKVAVEGNLVNIDAERGDKKYQTKVPIKHKVDVDSVKASYTNGILEVQFKLKKEDKPK
                     GKTVEVN"
     gene            3354..5492
                     /locus_tag="VGA92_05905"
     CDS             3354..5492
                     /locus_tag="VGA92_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX9845984.1"
                     /translation="MSEIILRINEVSQRHVGKGIAVIDPKVVKENNWQTGQIVELLAN
                     KKSHVKIWPGSTQDYGSGIIRIDGLTRHNIGAGIGEKVSVKLVNVVEADQIVLSPIEK
                     MSVEGLQEYMSTLYEGHVFTTGDTIMVNTHLGGKAQLIVTSTTPAKPVIVSPKTTFKL
                     GDITKAVDNSVPRITYDDLGGLKKEVQKIREMVELPMRHPELFDKLGIEAPKGVLLYG
                     SPGTGKTLLAKAVAGETNAHFTAISGPEIMGKYYGESEERLRSIFKQAEENTPSIVFI
                     DEIDSIAPKRDEVTGEVEKRIVSQLLTLMDGMKSRGKVVVIAATNRPDSIDPALRRPG
                     RFDREIEIGIPDEGGRLEILNIHTRGMPIDEKVNLKQIAKVTHGFVGADLESLAKEAA
                     ISSLRKILPEIDLDQDKISAEILQKIRITDDDFKDALKEVRPSALREVLVQIPDVSWD
                     DVGGLESLKEELRETIEWPLKHKEAFEYVNVSAPKGILLHGSPGTGKTLIAKAVAKMT
                     ESNFISIKGPELLSKWVGESEKGVREIFRKARQAAPCIIFFDEVDALVPRRGGHDSSH
                     VTESVVSQILTEIDGLEELNNVLVIGATNRLDMVDPALLRPGRFDRIIEVPKPDARGR
                     KHIFEIHTKKKPLDSDVNMSRLVQITEGYTGAEIAAVCNRAAIAALKRHVSGQVQSLK
                     DIKITQKDLIEATSKVRVPTTMSSTANPIA"
     gene            complement(5631..5930)
                     /locus_tag="VGA92_05910"
     CDS             complement(5631..5930)
                     /locus_tag="VGA92_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9845985.1"
                     /translation="MPVAILPDIGEQMCIGCALCVEICTTLGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACIGVCPTKAIFWMRPMDFTVGQPVPLYKNSVFVKGWTELID"
     gene            complement(6065..6688)
                     /locus_tag="VGA92_05915"
     CDS             complement(6065..6688)
                     /locus_tag="VGA92_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845986.1"
                     /translation="MHKNQQNDSTDIVAKIQKIIYNERLSSISFEDAITKTYFFHKLT
                     SITDMPTLYLDFDLLYSGYVAAKILSQSQNVILHQPTQQTWKNLCVDIMDQISQKQHL
                     VIIDSLNGFFTTITNQKDSGRIINNIIMLLVGTGQKTNSAIVVGSISKFKKDEGWILS
                     AIGRHVIEIDKMNLISVRKQNSHLQLVLADHHNLEKSSLQISELDLL"
     gene            6718..7872
                     /locus_tag="VGA92_05920"
     CDS             6718..7872
                     /locus_tag="VGA92_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9845987.1"
                     /translation="MNLDQELISHSFVKSERTYLNNASSSLIPLQTMKAMTDFTIQYN
                     ELGPDSLDFAALLKEKTIRLREAISKLVKCRPEEVVFTQSTTEGINAVSNGMSFPKNS
                     NMIIRGSTQEHHSNYFPWLRLSKNVELRSLAIDESGFFEISELEKLVDENTKLVALSH
                     ALYNTGAVMPVTEVGKILREKNVPYFLDAAQTVGCIGEFDFSKIDANFVAFNGYKWLC
                     GPMGIGIFVCKKDSAGLLEPMQVAGESSMLYDKDQLAHKDIPDKFQASFRNFVAVVGL
                     ESSVNFLLHFGLENIRHKVISLANLLRDELAKIPEVTLYGPADQQKRTSIVSFTVSKK
                     SPQEVVEYLEQQRIILAQREISDRKIVRASPHFFNTENDVLRTVEAIKKL"
     gene            complement(7867..8127)
                     /locus_tag="VGA92_05925"
     CDS             complement(7867..8127)
                     /locus_tag="VGA92_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9845988.1"
                     /translation="MPTAFILLNSDLGSDQEIVTKIKEILNVEKGLKFEVQGVYGIYD
                     IVVKIESENADHLRSIITNKIRKIDKVQSTLTMMVIDEQEQL"
     gene            8202..8843
                     /locus_tag="VGA92_05930"
     CDS             8202..8843
                     /locus_tag="VGA92_05930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9845989.1"
                     /translation="MRPQDLTLTVSEKIPTFPGSPQPHFIEWAELKKDHYNLEMLFLS
                     SHTGTHVDAPYHFVKNGKKIHQIAPRRFLGDAILIKISTRPNYGITKSDILKFEKKNG
                     KIPNGSTVVFATGWNNSLQRKNFFKENPGLAASAAKYLASKKTNMVGIDSPSIDVGKD
                     SSFSAHHILLENDVLILENLCNLNKIKSTRFKLVALPLKLQNATGSPVRAVAF"
     gene            complement(8840..9898)
                     /gene="amrS"
                     /locus_tag="VGA92_05935"
     CDS             complement(8840..9898)
                     /gene="amrS"
                     /locus_tag="VGA92_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX9845990.1"
                     /translation="MNKEAILYEKLPDSRVRCTACARYCEIKDGQIGLCGIRGNEGGK
                     LDLFVYGKVITGHVDPIEKKPVVHYRPGTRIFSIATTGCNWLCKFCQNYDISQRRKVE
                     GADMTPEQVAQMAVDNGSDGIAYTYNQPSIFIEFARDCGIEAHKKGLFNIFVSNGYDT
                     PQTVKMMDEFLDCITVDFKGSAEPDFTRRYIGVPDPKPIFDTLLEIRDKTKIHVEITD
                     LIVPEVGDNLEYTKKLAKFLYDEFGPEMPIHFLRFHPDHKMMEFPPTPTKTLEKHYEV
                     AKKEGLQYVYLGNVPGHPYEHTYCPECKNIAVGRYGFDITAWNLDANNKCKFCGKQIP
                     IIGPLDKKYKKNRFQFVI"
     gene            9973..10470
                     /locus_tag="VGA92_05940"
     CDS             9973..10470
                     /locus_tag="VGA92_05940"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase 23"
                     /protein_id="HEX9845991.1"
                     /translation="MSKPGNFWRKIHGRVTKRPTNFSWLLQDKLAGSGMPTTFEEINW
                     VRKQGVKSIITMTERGLPDSWVEGLEYLHIPTEDLSAPDIDKIDATVDFIHEQIKNKD
                     PVMVHCAAGIGRTGTILASYLIKYQNLTAKEAIKKIRQERPGSIQSESQEMAVTFYEK
                     FLKSK"
     gene            complement(10505..10657)
                     /locus_tag="VGA92_05945"
     CDS             complement(10505..10657)
                     /locus_tag="VGA92_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845992.1"
                     /translation="MAKYDGLLGQPVLEVEEPDTEGGVTLIFKDNRFLFVKLVDGKIE
                     TTSIPE"
     gene            complement(10688..11227)
                     /locus_tag="VGA92_05950"
     CDS             complement(10688..11227)
                     /locus_tag="VGA92_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845993.1"
                     /translation="MGSEKTFISEVSLTNDKDEYSNSDDIVIGIRFSIQGALREAFNE
                     KNWTDSYNKFDNLFKLKYGIKLVSGGFRKRELKSVDTYRKASIFWTRNPKLVNPMKEK
                     RVWVQVAKNFEPIIRLTEDEVRGELFDFDEKLTFKASELGSGKHKIGAEIYASWQKHH
                     FSDPDDVKSNAEEIEITIK"
     gene            11272..11553
                     /locus_tag="VGA92_05955"
     CDS             11272..11553
                     /locus_tag="VGA92_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoL/Rpb11 RNA polymerase subunit family
                     protein"
                     /protein_id="HEX9845994.1"
                     /translation="MNAQIVKSSTKETNLSIRDSDIGTLYIVQHELLKDQQVEFAGVI
                     LRHPLTSEYSMRVNSSKGNPLKEILKATDSAIEFTNGLKQTIHSKFKGV"
     gene            11554..11853
                     /locus_tag="VGA92_05960"
     CDS             11554..11853
                     /locus_tag="VGA92_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HEX9845995.1"
                     /translation="MQFCSTCGVRLKKGTCQKCGYSEGESKQEPKKLTEQSQSELTIL
                     EETDGKEALSSITIDCEKCGNTEAVWWMLQTRSADEPTTQFYRCTKCNHTWRNYA"
     gene            11944..12687
                     /gene="pcn"
                     /locus_tag="VGA92_05965"
     CDS             11944..12687
                     /gene="pcn"
                     /locus_tag="VGA92_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030337 - DNA polymerase processivity
                     factor activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proliferating cell nuclear antigen (pcna)"
                     /protein_id="HEX9845996.1"
                     /translation="MVFSAKTSGSDEWKAILSAISTLVEEATFEATAEGIAFRGMDPS
                     HVALIDISWPNSAFEKYECDGDIKFGVRIDEFSKLIKRAEKSDGIEISLSENMLLISI
                     GKNKQYKMRLIESSATDTPLPKIPYDTKIGLATVLFDKILGDVQVVSDYLTIKTTSTK
                     SEFSGKGDAGEVLISLQKQQGELSEISVKEESVGTYSLEYLNPIVKAVGSASELIICE
                     YSSAKPLRIEFKVANMGRIHFYLAPRVES"
     gene            12724..14133
                     /locus_tag="VGA92_05970"
     CDS             12724..14133
                     /locus_tag="VGA92_05970"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9845997.1"
                     /translation="MRLVLKFGGTSLSSAKQIKAVAKFVHNVSKKNQVTVVCSAINDV
                     TDQLIQISEFIQKRNKAGASSTLAKLKKQHQLIAKDSISNAKVRKKLGEKLNANLAEL
                     EGLLHGMMLLGEVTPRSLDYLISFGERLSVGIVSYVLQDLKSKSIDLTGKDVGIITDS
                     NFGSSKPLMDTTRLRVSANLEPLLSKKIIPVIGGFAGADQHGHITTFGRGGSDYTATI
                     IASCIKADEVWLMSDVDGLMTADPKIVKNAKVIPEVSYVEAMEMALFGAKQIHPRSFE
                     PLLTKKIPLRIRSTFNVNNPGTLVTATPDESTNKTVKCVSVIRHNGLIDMRGGSMVGA
                     PGTAATIFSALAKAGVNIMMISQSPSESSISVVVKKNDLDRAVNTMEMNLLGKIIKKI
                     DVTADVAIIALIGSGMRGTVGVASKVFGATARKKVNVVMIAQGSSELNLAFVVKDSDC
                     AVVVQALHEEFKLAEQISQ"
     gene            14172..15629
                     /locus_tag="VGA92_05975"
     CDS             14172..15629
                     /locus_tag="VGA92_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9845998.1"
                     /translation="MRKLAIISVIVSVTLVLTALSMLSDELAVAKPQSKVHFTKTITS
                     SQDPGVGQGSYEFALVLSPNQGSLYTGSLTYTASEAVEVVVLHELDKTDDGGQPTWTV
                     DGNTIYAMSVIEPSEARSFDFTGAAIAFRSQNPFIVTASVDGWIRGQPVELVIQKLEI
                     KEKSLSLPDARVPVTIPMKVGFFGKDSVYYILTDSSNKTLAEKITEKQGWPVQFAPKL
                     RWSPASSQDTIYVFTNGVTGDGIHGFQGEVFSSTPAQTESYSPLRTLVSVSWKEGQNP
                     QILDTAEDILKAEKDSRIKLVRTNVTINAPQVAWPGGQLFVRNETTLSNETFEKGQVI
                     GIDKDAKKVTFVAHRGWGFDGRTIYYIMPDATPKGPADLMGIPVSPKLANVLSGVVFA
                     DMYQFKNGFKGPGQLGFQASIISSKMDESYVPICRVSIVEWKEPDSALVLETISDINK
                     KKSDGSIFVTLARPLSDDHVVNCPLVELTNLSNKG"
     gene            15658..16431
                     /gene="cobJ"
                     /locus_tag="VGA92_05980"
     CDS             15658..16431
                     /gene="cobJ"
                     /locus_tag="VGA92_05980"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX9845999.1"
                     /translation="MSGKLYIVGVGPGSHDHMTFRAKQVIEESDTIVGYDTYVSLVED
                     LIKGKDVYRYAMTQEVERAKQCIDLAMEGKIVSLVSSGDPGIYGMAGLIYETLAESGW
                     DPESGLEVEIVPGVSALNSCASLVGSPLMTDFAVVSMSDLLVPWEIITKRVEAAAQGD
                     YVIVIYNPSSKKRIHQLQDTRKILLKYRKPTTPVAIIKGAYRDSQVVVITDLDNMEKH
                     ADKLGMISTVIIGNSSTYNFKNLMINPRGYTSKYNLQTN"
     gene            complement(16435..17472)
                     /locus_tag="VGA92_05985"
     CDS             complement(16435..17472)
                     /locus_tag="VGA92_05985"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9846000.1"
                     /translation="MDVAQKIELISKPPTEEIVTKEELTNLFETNSKPKHYIGLEISG
                     FLHLGSLISTGFKINDFIKAGVQCNVFLADWHTIINEKLEGNWDIISKVSNYYEKAFK
                     LVCPGVNIIKGTELYDSHKEYWSNLILFTKHMSLARTMRTLTIMGRSENEEKIDLSKL
                     LYPPMQAVDIHTIDVDIAHAGMDQRKIHMLVREIFPKMKWKVPVAVHHSLLPGLSEPV
                     VEDDAGTKMSKSKPSSGVFIHDSDDEIRSKIKKGWCEVGNLNNPILQIARHIIFHEFS
                     EITVERPEKFGGNVTYASYAQLESDFGAAKLHPSDLKQTVANHLVKIIAPIRDKIALD
                     EELYNTIKNSI"
     gene            complement(17535..18008)
                     /locus_tag="VGA92_05990"
     CDS             complement(17535..18008)
                     /locus_tag="VGA92_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9846001.1"
                     /translation="MTKPIPDGFSTVTISLIVKGAANAIEFYKKAFGAQELYRFPGPD
                     GNSIMHAEIKIGNSIVMLSDEMPQMNCLSPQSLGGSGMAIYMYVEDADAVFNQAVTAG
                     AKPIMPMMDAFWGDRFGNVQDPFGHIWGIATHKKDMSAEELQKAGQEAFKNMCSK"
     gene            complement(18072..18638)
                     /locus_tag="VGA92_05995"
     CDS             complement(18072..18638)
                     /locus_tag="VGA92_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846002.1"
                     /translation="MKVHHLQEDEKGYRVFLYIFYICAIMMTSITFYAIYAMMQEWQE
                     NGTYVMGEVLVETEFPFPHFAKLITWLFFAIIIGWYCVSRIGWKKTTQLYSWKMSLLQ
                     LMLLGFCVICLYEVLYNFTVLEAQISAGIENGKVPDIDSLTIAYPDPQRPWNLIFATK
                     IFLAGFLISAHSFYLSTRPRKSLDDLNP"
     gene            18719..19804
                     /locus_tag="VGA92_06000"
     CDS             18719..19804
                     /locus_tag="VGA92_06000"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9846003.1"
                     /translation="MKVPVNIPFLGKEEIAEVNSVLKGGTLTSSASLGGKNVQGFERL
                     VCSFIKSKYAIAVNSGTAALQAALYAFGIKSGDEVLLPSFTFVATANAVVSVGAKPVF
                     VDILKENYTMSPSDLKKKITKRSKAIIPVHLYGNVAYIDEISEIAKKHSLDVIEDAAQ
                     SMGSSYKGKQTGTFSEIGCFSLYAAKVMTSGEGGVVVTSNKKLWEKLLMIRNHGMVHG
                     YDTRVLGLNLRLPELNAAIAKVQMKKLPKFLQKRQKNAKILTELLTGLDIGLPHQRKY
                     EKTNWYLYTIATKARNKIMKELNAKGIGATVYYETPVHKTPFYNKKIRLENTEWAASH
                     VLSLPVQPMVTQHDLELTAKTIRSVVT"
     gene            19801..20277
                     /locus_tag="VGA92_06005"
     CDS             19801..20277
                     /locus_tag="VGA92_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HEX9846004.1"
                     /translation="MNSLVELAGQLCKILLPIDEEVFYGDPSSNVAICTLSSMSLLKE
                     ISNSDLMTEITLVGRLLSENKGIDALIRHVSKHPTLDTIIICGMEVSGHRAGHALFCV
                     HKYGTDEQNRIINSVSPDPVLSVSKSEIQQFQRQITLINKIGQTSLEEIRSVMSSQ"
     gene            complement(20271..20600)
                     /locus_tag="VGA92_06010"
     CDS             complement(20271..20600)
                     /locus_tag="VGA92_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HEX9846005.1"
                     /translation="MDKLELIFSLQKGLAEMMNLDRYPKDVEGRISALCTAIIHEAVE
                     LQRTTNWKWWKKPIPFNIADAKEELIDVWHFVVQASLELNLTPEDIIAEYKRKNEINR
                     DRQRNGY"
     gene            complement(20633..21526)
                     /locus_tag="VGA92_06015"
     CDS             complement(20633..21526)
                     /locus_tag="VGA92_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9846006.1"
                     /translation="MLTVFGSTALDTIRTPTKILKEVLGGAATFAGISASFFVNTGLI
                     AVVGTDFPKKYHDTLSKYLDLSGFAVKKGKTFRYDGSYDLTLSKRTTNKTDLNVLGSF
                     KPTVPDEYKKSEFVYLANNDPDQNSSLIKEFDNIKFSMCDTIEYWIATKRPSVIKMIK
                     AVDAVVINDEEAKLLTKEHNLIKCAKKMMGWGTKYVIIKKGEHGSLLFVEDVIFPSVA
                     FSLEDIVDPTGAGDSFAGAMIGYLASKKTTSLSEIKKSIIYGNVLGSFAVERYGVDGL
                     LQINKTKIENRIKQYQKMIGF"
     gene            complement(21538..21990)
                     /locus_tag="VGA92_06020"
     CDS             complement(21538..21990)
                     /locus_tag="VGA92_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846007.1"
                     /translation="MTKKKKATIIAAAIIGVILFGFTQYASAHQITVVITDSELIQKT
                     EQGSLYNLELEFSNPSLLFLNAGKTEFTIAADDDTLGGGELDPFLLPALGKATTSGTF
                     LKDHSANSENPQVKIIGVTKYQLLFASIEIPFTYYPTESQTSKFIHDT"
     gene            22124..22612
                     /locus_tag="VGA92_06025"
     CDS             22124..22612
                     /locus_tag="VGA92_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846008.1"
                     /translation="MAKIRLRFGENEVEIESRDFYIDNDTAGQVISELARNLENKARI
                     ITDNEISLEPPQQIRQTYQANVDYLRMLKDAEVHEPEFSPPMPVSSDEIQSKIESLEN
                     DSFFLKPRTVSETVEQLREYGWLASPLDVSKILAKMAFHRELAKDSQENKTYYFKKQI
                     IV"
     gene            22859..23014
                     /locus_tag="VGA92_06030"
     CDS             22859..23014
                     /locus_tag="VGA92_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846009.1"
                     /translation="MAKKRTIPIEIDDHFKIYGKEPWEVDYGEKCPICNVRIDEYGFC
                     SCGSSGD"
     gene            complement(23001..23897)
                     /locus_tag="VGA92_06035"
     CDS             complement(23001..23897)
                     /locus_tag="VGA92_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846010.1"
                     /translation="MKKIILGLIIVTLFSTTSTVFAQEVNFGQPAVQSVQITINEDSS
                     AHVVHIVEPSGNSRQLSVISNDFINLQITDQDGDSVEYGEIGGDRTAFLILPTKKKVN
                     VEYDLADVISEKNGMMTWDYHYLASTSFYLPTKADLVFVNGNPINLSDLDGIRCHGCQ
                     VILEYQLDKTETVEQVQWEDKKFDVRIITNTDISSFEFDQPNKKISFDVSDANQYITL
                     IIPLELLWNPYEVFLNENKMLKHEFYSDEKNVWLNIKTNETGTVEIIGVSAVPEFPFA
                     AVLILATAMIFAARYTNNFNLR"
     gene            complement(23907..24386)
                     /locus_tag="VGA92_06040"
     CDS             complement(23907..24386)
                     /locus_tag="VGA92_06040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9846011.1"
                     /translation="MVNVTIETKFGKIIFKPLPDVAPETVRNFMNLVQSGFYDGTLFH
                     RVIPGFMIQGGDPNTKTQDKAKWGLGGPGYTVKAEFNSKSHLRGIVSMARATDPDSAG
                     SQFFIVTSDSTFLDRQYTVFGEVTEGINIADTIVSQQRDKNDCPLEDVRMLKVSVSE"
     gene            24633..24866
                     /locus_tag="VGA92_06045"
     CDS             24633..24866
                     /locus_tag="VGA92_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846012.1"
                     /translation="MNRTNQSTVIKQAINSYLEKGLTDKQDIYSKVVEELGVPRPTVR
                     RVARDLRNELLVKIKILQSETPQAESSAQEKDE"
     gene            24955..25422
                     /locus_tag="VGA92_06050"
     CDS             24955..25422
                     /locus_tag="VGA92_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846013.1"
                     /translation="MGYLRNQLATVVVSAFAAFLTGMYFVLPPILNFFMLVVVIVTWF
                     LAVMCWIVQKGTDYSHRHSQQHGYSEKKSSASLVTSTYTKTVSLDELTKFKESFTVEL
                     NELKDEVKLKDGEISNLRQQIANLQTQVQIEALKVELANLKVLAAKEKSSKRK"
     gene            25596..26012
                     /locus_tag="VGA92_06055"
     CDS             25596..26012
                     /locus_tag="VGA92_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846014.1"
                     /translation="MVLKAVIASLVFALVFSTVSMAFADKVQTQTTITPMDESIGLEK
                     TTVPFHAPTQNKLPWGFVEGKIANPVEGYPVIIQIFKNGEAIHFAQTDVNEDGMYEYK
                     FRVRDVTNSRVTTIFEGDYTVKIFKVVYLDKVTTLV"
     gene            complement(25999..26523)
                     /locus_tag="VGA92_06060"
     CDS             complement(25999..26523)
                     /locus_tag="VGA92_06060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX9846015.1"
                     /translation="MKIVVLSGSPRKTANTQVMMKFVYEYAKSKNSDTKFINLSDGGI
                     DYYRGPNENYSQTTLQAAKDITEADVWLVGTPIYNSFFSSALKNMFEFINYKNTAGKT
                     AGIAILASGNIGFIDVQTLLTQLMSYFRVITNPKAVFMTADMVKDDAIIDENGKTRLK
                     ELVDETMLLASKLE"
     gene            complement(26568..26660)
                     /locus_tag="VGA92_06065"
     tRNA            complement(join(26568..26627,26646..26660))
                     /locus_tag="VGA92_06065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26605..26607),aa:Arg,seq:cct)
     gene            complement(26693..27400)
                     /gene="bluB"
                     /locus_tag="VGA92_06070"
     CDS             complement(26693..27400)
                     /gene="bluB"
                     /locus_tag="VGA92_06070"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEX9846016.1"
                     /translation="MSDKFSNQEKSGLYKAIYTRRDVRSHFNSKPISDEALARILNAA
                     HHAPSVGFSQPWNFILIRDPITKNKVKNSFEEERKDSSKKVEEPRKEKYLSLKLEGIL
                     ESDVNICVTYDPSRFGPFVIGRSSIPETGIYSVCCAVQNLWLAARSEEIGVGWVSILS
                     NDTLKQALDLPDHIIPVAYLCLGHVEEFAEKPDLETANWLPRLELKDVVYFEKWGQTE
                     RLSTWNQIYELIRTNLD"
     gene            27468..27541
                     /locus_tag="VGA92_06075"
     tRNA            27468..27541
                     /locus_tag="VGA92_06075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27502..27504,aa:Val,seq:gac)
     gene            complement(27570..28061)
                     /locus_tag="VGA92_06080"
     CDS             complement(27570..28061)
                     /locus_tag="VGA92_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846017.1"
                     /translation="MIRGKGILYAAIGALLAMLGGFTWYASLDNPQLDMAEIQLTTVK
                     VQNVNNIENRATLEVIFLIRNPSEKTFTVSIIDYELFANGKPIGKGQYSTEDIPLPGR
                     AAFYPGAEIELPNTFQLIQSGDNSNEYDVIVNGQSVTFSAKGMMMVESAWSLVEKEFE
                     TSQ"
     gene            28139..28633
                     /locus_tag="VGA92_06085"
     CDS             28139..28633
                     /locus_tag="VGA92_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX9846018.1"
                     /translation="MALEQAIITWIHLISSAIWVGGSLFIAVVFAPVLKTMSSSVEER
                     LQIMVKVGRKFNKIAMPSLMILIGTGIFNSHQILSRPDFLFSTTYGILLVIKIILVAA
                     LLASFVVHVRIIRKDVEQKIMEKQLSEVQIVRLRKKIIVVGQLTVGLSIVILLVAALL
                     DAGI"
     gene            complement(28613..29644)
                     /gene="rtcA"
                     /locus_tag="VGA92_06090"
     CDS             complement(28613..29644)
                     /gene="rtcA"
                     /locus_tag="VGA92_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX9846019.1"
                     /translation="MESLKIDGSFGEGGGQILRSAITLSCITKRPIQIDKIRHNRKIP
                     GLRAQHLTTLSLLAKICNAKTEGLQIGSTSIKFFPNEIQDMRIKESVGTAGSISLILQ
                     ALIPAVSLAKKKLELSISGGTDVPWSPTANYTKYVLAEAYSRIGIDFLMKIQRRGYYP
                     RGGGLVELTVFPSKKLLPLFLTKRNERNAKILCSYSNLNASEISRAVDHVRSTLEKKE
                     FSAQIEINEEKATDKGASMLVLSHDLSSIIGSDELFDKKSAEFGNKSASTFIGCDLGV
                     DLHLSDMLVLPLSLTKGLSIFTVKEISKHLETNLYITSKITGCKYGVGKIEGGYEVRI
                     EGNSDSCIQ"
     gene            29712..30206
                     /locus_tag="VGA92_06095"
     CDS             29712..30206
                     /locus_tag="VGA92_06095"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HEX9846020.1"
                     /translation="MNGLFIGRFQPFHLGHLAAIKFALSSVDNLSIGIGSSNRSNEKR
                     NPFTADERKEMILSSLDESTLEKIKIYYIPDVDDHEKWTYHVDSIVPKYDVVFSNDDF
                     THNLFKKRGVKIVSVPLEQREVLSGTEIRAKIVNDQNWYEFIPDGTRKVLLKINAKQR
                     LKNL"
     gene            30249..31394
                     /locus_tag="VGA92_06100"
     CDS             30249..31394
                     /locus_tag="VGA92_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX9846021.1"
                     /translation="MEYLVMLPGPTNVPERVMRAMITPMINHRSDDFVELYEDAVEKT
                     KQVFQSNGEAVCLSASGTGAVEAGVINLIKKGDKVIIPVNGEFSGRLAQMLEWAGATV
                     IKLETQPGENASFEAVKEAFDNNKDIKAFYCVWNETSTGTMIKYLDRVKDLTSRNDSY
                     YVVDGVSIVGGEELKMDKWGIDVAMTGAQKAFAAPPGISPIVVNAKAKKYMIENPPKT
                     MYFNLSRYFKYYEESKHTPFTPALPLLYAYREALSIILEEGLDNRIRRHRICSDALYS
                     GLSAMGLTPFAKEDARSTVVVALNYLQGLEDKTFRNTLAQKFRVLVAGGFGDLKGKVF
                     RVGCMGEVQRYHVMRTISAIGSTLEMMGYKTKSVDALKVAEEKLKAL"
     gene            31437..32165
                     /locus_tag="VGA92_06105"
     CDS             31437..32165
                     /locus_tag="VGA92_06105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9846022.1"
                     /translation="MTFIKGSLILIDYTAKVKDSNEVFETTIEEEAKKHSILEANLKY
                     QPKLVSVGESWVIKGLDDALTNAKAGDKLTVEVTPDKGFGERDPSKVRMIPLRKLGED
                     AEKVSVGDTIEIDQKTGIVRFIGSGRVQVDFNHRFAGKTIIYDVNILKSLESDEEKIT
                     AMLKRHIPVEDSKIVSKLNGSSLDVVIPEEIFGAEGLRIIKHFIQIDVFKFIPTLEKI
                     NFIETYNNKKTEKNKEVPLETKAA"
     gene            complement(32162..33208)
                     /locus_tag="VGA92_06110"
     CDS             complement(32162..33208)
                     /locus_tag="VGA92_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HEX9846023.1"
                     /translation="MQDTPSHTMELPRLIVVGEKNIGDFGEFLRSLGKPKKVSVIAGP
                     HVQKIVKKKLQKSLSESKIRSIWHGPKDNDEKSIRQVQNEIKKDKSDLIIGIGGGRSV
                     DVAKMIAFNLQRPFVSVPTAASHDGIASPFVSIRGERPHSLVATAPLGVFVDIDIIKR
                     APKRLLASGCGDLVAKITAVKDWEIGRDKTGEYFGRYSANLASMSAKILVEASEKKKH
                     PDIREVVEALISAGVASCIAGSSRPCSGSEHLFSHALDKIAPGVGLHGEKCGIGAILM
                     AKLQGQDWKKISKTLKNAGAPTTAKEIGLKRNLLAKALIIAQSLRPERYTILKEVHMT
                     KTKALDLAKSTCVI"
     gene            33307..33942
                     /gene="psmB"
                     /locus_tag="VGA92_06115"
     CDS             33307..33942
                     /gene="psmB"
                     /locus_tag="VGA92_06115"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HEX9846024.1"
                     /translation="MSSNNVEEKILHGTTTVGIKASNGIVLCADMRASAGYFIANNNT
                     MKIQQIAKHAGMTMAGGVADAQNITDVLRYHANIHKVQKNEDIPIRSLARLCSLLFHQ
                     NRGYPFIADILIGGYDKEGPALVNIDMFGSVEEKKYVTTGSGSPVAYGVLEDEYSDNL
                     KVEEAKVIALRAVKAAIVRNIGTGDGINVAIIDKDGFRLLTKEQKKAIITL"
     gene            33950..35887
                     /locus_tag="VGA92_06120"
     CDS             33950..35887
                     /locus_tag="VGA92_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HEX9846025.1"
                     /translation="MQRKQQEKEIHSTAQSVMATILQSIPKEAQVSKIDYEGPRIALY
                     TKSPLYLMEHNEIISDLVNVIKKRIVVRTEESIRKTEEDARQIIAKALPKEAELQGTF
                     FDNATGEMSVEVKRPWLLHNNPQFNIAQLTESAGWRLRIRKATTIPSSTIQAINYNLK
                     VSTSDRAKHLKQIGENIFRPRLVQKSEVSLLTLGGFGQVGRSSMMLVTPESKILIDCG
                     INPGARTPLESFPRLDWANLTLDEIDAIVISHAHLDHTGFLPVLCKYGFKGPIYCTEP
                     TLPMMNLIQLDAIKVAAAQGRAPIYSERDVKQIMRQTVTIPYGTVTDIAPDIKLVFSN
                     AGHILGSATCHFHIGNGDHNFVYTGDLKFGKSILFESASWNFPRVETLLIESTYGAKE
                     DIQPTRQEVESAFINAVNNTLVDGGKVLIPIPAVGRAQEIMMVIDYYMKAGQMVEAPV
                     FMEGMISEASAIHESFPEYLARELRQKILETDDNPFDSEYFTNIEHSDAREEPLREGT
                     PCIILATSGMLEGGPVLEYFRNIAPGNKNKIIFVSYQVNGTLGRRVLDGAKQVPLLGR
                     DGKVEIVNINCSMIKLDGFSGHSDYNQLLSFVHKLRPKLRRVLVNHGERRKCENLAMT
                     IRRMFRIPSHYPQVQEGIRLF"
     gene            complement(35899..36990)
                     /locus_tag="VGA92_06125"
     CDS             complement(35899..36990)
                     /locus_tag="VGA92_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846026.1"
                     /translation="MEKFLLVITALSIIVILNTKPSAAETPVGTDEKIILIQSCEKIY
                     PDLEKLGTEKFRQRYQYHMNFRSCTILFNDLLWYSNDTDRTEKLVALLDKPTVPMPVR
                     DRLTQSQTIPEWIKDDAIRWQQGKEKDTVFSYGIRYMITSDMIPALTNISDPNYCENR
                     ICAVQNDFLKYSIKNTGIQDVITLTHTFQNVNENSLIILAQEVSKSGKTTNTFQINED
                     GLASLADQKCCDYYRFMHKMPLQIGTKIGSTFDAQLTDEIVYPFMDQKRPALIAKDHT
                     DSYYEIIDKQTGAVLFAKHQDRIRKTIWTAELVETNIFTKDIKIQYEDMRIPPWFKNV
                     VRWWTEGKITDTEYLSGLGYLIKHDVLYV"
     gene            complement(37056..37334)
                     /gene="sepF"
                     /locus_tag="VGA92_06130"
     CDS             complement(37056..37334)
                     /gene="sepF"
                     /locus_tag="VGA92_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX9846027.1"
                     /translation="MQKQESPTYLKAITIRDPSDIHSIKEDMKKNMILILRVTPLAQK
                     DVEQLRKLVEDLYAQAKNYNADIARLGEERIIVTPPGVKIWKPEYDLK"
     gene            complement(37409..38422)
                     /locus_tag="VGA92_06135"
     CDS             complement(37409..38422)
                     /locus_tag="VGA92_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HEX9846028.1"
                     /translation="MLEEQKHKVVALLSGGLDSQLAVKMMQKQGFEVSAVAIKTPFCD
                     FDCGRGCGFEIRERADTLGVNLKTVYLGDEYIEMLKNPKHGFGSGMNPCIDCRAMMFK
                     AAKKHMDEIGADFIISGEVLGQRPMSQHGPALKTIEKESELEGYIVRPLSAALLPQTM
                     PEKNGLIKREDMGMIRGRTRRIQLQMAKEFGIENPPNAGGGCLLTDPAFAIRAKDLFK
                     HITTPTTNDIDLLKIGRHFRLDEKSKLIVGRNKDENEMIKALALPDDLIFEVKDHMGP
                     IALLRGEMVETHKDLCAAITLRYSDAPKETQGIVSIEKGNGTKSEILTNKAEESDYIK
                     YRI"
     gene            38540..39604
                     /locus_tag="VGA92_06140"
     CDS             38540..39604
                     /locus_tag="VGA92_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX9846029.1"
                     /translation="MKERRKKLMQYAQDMNCNVLVSFEPENLFYMTGFWGEAVGILEK
                     DGTATIIAPELEVDRAKKESIDCNVIKSERGVGLLSSIITKINGKKVCTDCQNYVAME
                     SLKKSIPSIKHSTDPFYRAREIKDQTEVSTLRKASKVIDQLYELCVKKIVVGQRESEL
                     QAILMSYAMEHNAFSTGYRSTLNPLIIAGGPNGALPHAQVTKRKFTSGDLIVVDLTLR
                     YNGYVSDATRTFGLGTISDQVKEVYEIVKESQRKGLKIVKPNISCKSVDDACREYINQ
                     KNFGQYFIHSTGHGIGLEVHELPTISAKSQTKLQKNMAITVEPGIYIPKKFGVRIEDS
                     VIVKDTPQVLHKFTKDLIIL"
     gene            complement(39720..40583)
                     /locus_tag="VGA92_06145"
     CDS             complement(39720..40583)
                     /locus_tag="VGA92_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9846030.1"
                     /translation="MGNPKKIKFNSIEYKRRIITTWNEIAPKYHKRWAKDNIGPFKST
                     AKLVSMAKIRYGYKVLDLACGTGVVTKQILKKVGNSGQVTGIDSSQTAIKIAKKWNNA
                     KPNVNFVIADAEHIGFNEKFDTITCQYALFFFPNTNTVLKQIKHCLKKNGTLAVSVHG
                     EKNSVPYFSSILDVVIKFVPDYIPPGAPSLDRFGTKTSLRKALAKADFTNIEIKQHNF
                     EYRPGTFSNYWNDYLRYLAKPLKEKIYKIPIHQRHSMRERIRKNTLPYTKESGQIVFP
                     WKVLIATAKNP"
     gene            40627..41055
                     /locus_tag="VGA92_06150"
     CDS             40627..41055
                     /locus_tag="VGA92_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9846031.1"
                     /translation="MSTKILDANIREGRLHDITHFGIRLAVGVIFIVHGSAKFNPGFV
                     GFLSNIGLPAEMQIPIALAETVCGILLIIGVLSRISSSLLSIIMLGAIFHVKGAASLT
                     GERGFEIDLILLAANLTVIAIGPGRVSLSHLVKKIPRFLQ"
     gene            complement(41039..42604)
                     /locus_tag="VGA92_06155"
     CDS             complement(41039..42604)
                     /locus_tag="VGA92_06155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9846032.1"
                     /translation="MKFSLVVLVVIGVFAAGVIPVFAETEISDKVVVLHTNSGDMVID
                     LFPEDAPKTVANFLNLTEHGFYDRTVFHRVIKDFMIQGGDPKTKPGAYKNVSEWGTGD
                     AGYTILGEFNTIKHKRGIVSMARGSDPDSGSSQFFIVHKDSFFLDQNYAAFGRLATDT
                     SYETLDKIANLETAGEAANGIPLKWGEAEVLSAEIKNRSEIPDLLDLGDPERVSALTN
                     QTAQEYSNEKLGFSFVPPAGWFVQEPPKRNPQTPDVVAAGSKIGGFTPAISFSVKNSN
                     GTSLESYSASIKKTLQKAIDDGTLTILMEEKTSINGNEAYITDAVGKFDTPEGIINVK
                     FKEIIVKSSETFYIITYSNSENNFDETLPKFDEAIRSFKITSPPTYSDHAAPITDDKN
                     GGCLIATATYGSELAPQVQLLREIRDGVVLNTNSGSSFMIGFNQFYYSFSPTVADWER
                     QNPTFKELVKTTITPMLSTLSILNYVDIDSERELLGYGIGIILLNMGMYFVVPAIIII
                     KTKNRFQRFIAEI"
     gene            42880..43305
                     /locus_tag="VGA92_06160"
     CDS             42880..43305
                     /locus_tag="VGA92_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846033.1"
                     /translation="MKQTQTLVLVIVGINVLAIVIATNLIQQQTEAEKERVRHEQQIQ
                     EIKNAFLENDIKKATNLIERTELNEEDVRELDWIISQEIDRRLDSLTKSLDNWSLPDC
                     TPAQRVMLDSLEKRLEQSQTIKEAIIHQNQINNILSQCR"
     gene            complement(43664..43873)
                     /locus_tag="VGA92_06165"
     CDS             complement(43664..43873)
                     /locus_tag="VGA92_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846034.1"
                     /translation="MPKMERENMNLKCKYCRQIIYWQYRNGKWYAFDDMCNWYLHRCP
                     EYEKRESMPYNRKIIFDAIGEGVRL"
     gene            complement(44222..44344)
                     /locus_tag="VGA92_06170"
     CDS             complement(44222..44344)
                     /locus_tag="VGA92_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846035.1"
                     /translation="MSKFTTINLNPSTKERLAKLGRFGQSYEAIIIELLGRAGH"
     gene            complement(44422..>44895)
                     /locus_tag="VGA92_06175"
     CDS             complement(44422..>44895)
                     /locus_tag="VGA92_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846036.1"
                     /translation="SDSELVFANQKNNVAAVRVEEAVFSKLRRRCGLLEKYEGSSRFV
                     VNIHAFRAYFHTNASRIHSTEYANALDGHTSYLGQYYRIAPEERAKMYKTLEPALLIF
                     GNSDATNGLFKEQLESKNKEIEHLTKLIIEMKEDLEKVRKRQERLEKTIVVKETK"
BASE COUNT        13539 a         8533 c         8925 g        13898 t
ORIGIN      
        1 aaaaagtaaa atctctccat tttgtaaaaa tagccagcat tagaagtgtt agcgttttga
       61 tttttcagtt aaagttcaaa gctgataatt aagctaagca gctgaaaaaa ttttaaaaat
      121 cttttcaaag attgtataat atgatacaaa tgcccattaa gaatctgact gcaaaatacg
      181 ctgttgccgt aatgggagca atagcactat taatgctctc attcggttat gcggcagctt
      241 tctaaattag gcgcatttag cgccattttt ttaccaaaca tcatcaacct catcaagctc
      301 tttgtaatca ataggctgtt ctttacgaat tatctttaat cttgaaatat cacgatcgaa
      361 gagcaaatct gcagtccaat caagaaaaat tcttagtctt ttgttaaact tgggcacctt
      421 tcgaaggtaa actgttctcc aaatccacca tgcaataaac ccatagatgt tcattccata
      481 gatttgggca attgcagttc gctttcctat gattgccatt tgcccttttg acatatagtc
      541 gattttcttt tttattttcc ccttaatggt agcgtaaagg ttgtatgctg caaattttgc
      601 ttccgcttct gcattttgag ctgttggagg atatggcttg ttggtttgag gattaattgt
      661 caagctgcaa tcaccaattg caaaagctcc tggaaattcg ggaacctcta ggtattcatt
      721 taccattact ctacccctat ctgttttgaa taaggaattc tttattgtat caacaggtgt
      781 tacgcctgca gtccagatca atgtctttgt ttgaattgag cgtagttccg ctggcattac
      841 tgcagttttg tcatcactga ctgcatcctt tatcaagacc tctgagccat caaaactcac
      901 aaccatggta tttagcatga tttcaattcc tcgttggatc agcttgtcct ttgtgaattt
      961 agcaagcttg tcagaaaaac ctggcaaaac tatcgataag gcctccagca caataactcg
     1021 tatttctttt tcagaaatgt ttggatagtg tttttttgca tcaagtaaaa agtcatgcat
     1081 ctcacctgcg gtttctattc ctgcaaaacc agctcctaca attacaaatg taagcaagct
     1141 ttgtcgcaaa attggatcag tttcgttttc tgcttgctct agcatatcga tgattctgtt
     1201 tcgcaaaaca actgcatcat ttagtgactt catggtatag gcatgatttt ccacactctg
     1261 atttccaaag aagtttgttt gactgcccaa tgccacaaca agatagtcgt attctaagga
     1321 gataccacgt ttttcgtttg ttccataaag tgttacgtac ttgccgtacg gatctatgtt
     1381 tttgactctg ccttcataga aggtcgtttt tttgcaaatt gttctaattg gtataatgat
     1441 gtggcgcgtt tcaatcatgc ccgaggcaac ttgtggaagc atgggcgtaa aaagcaaaaa
     1501 attgtcctcc gatatcagga ttagctctat gtctgagtct cgcttaaaat aggattcaag
     1561 ctttcgtgta cactctattc ctgcaaaacc tcccccaaga actaggatcc ttttctttgt
     1621 cacctccttt ctgccaagcc aatcgatata atgtttagtt cataaactaa catttcttgc
     1681 aaatttaact atctagaggt agaataaaat attacaaaga aggtctaaaa ttgtatgaaa
     1741 aaggaatatg tagtggttag aatagatgca gcacccgatg gcgcgccata cgtagttata
     1801 tcactctcaa gtgccaaaga ctttaaggaa aacaatcagc ttcctcaatc accacttggc
     1861 tccggcatga tgggattttc aaacatggat gacatgatga aaggactaaa caaaatgctg
     1921 actggaggag tttttggaat gccgggggga gtaacaagca tgaaacttga tatgagggaa
     1981 tacaaagagc tcggactctc agttggcgat agaattcacc tagaaattac aaagccagaa
     2041 aacacaggcg tataattatc tagagatttc tgcccgcaag acctcgtagg cttttgaagc
     2101 atccccttct cttaggacaa gcactatttc atcttgagaa aagaaggcgt tttgtagctc
     2161 aagtccattg ctgtgcaata actcggcaac atacgacact acatcggatc tgttttgatc
     2221 ctgtgaaatc ctaattttga tctttgctag tcctgcgttg agaaagttgt ttgtagatgg
     2281 aattgattcg aacatttttc gaatatcaac aagatcctct gttaaaactc ggaaagtaga
     2341 atctgccagt ctgaagaact cgtaatcaga gtcaaactgc tcaaacttgc tcaaaatcga
     2401 ggctgcatcg tttttgtcaa tatcatttgc agaaaacttg atgtccaaaa ttccatcagt
     2461 aagtgagagt cttgcgtttt ttagaataga ttccgacttt agatcatctt tttcgacaaa
     2521 ggtatctgaa tagcgcttta ttgcaacaac aattgtgttt aggttcacag gattaccaat
     2581 ctgcctttct acgtctggtt gaattttaac ggcaagcgca gtatagttga tgacgtccat
     2641 tttcatacag tcgtatatag aacggttcct tgtgataatc tcgcgtactg tttcgggaac
     2701 cgacaaactg ctagtcctca cagaattatt tatataatgt catatataat agtcttctgt
     2761 catattatat ataaaacaac aaaatattta tataatgtca tatatattac attatatagg
     2821 tgaatatgac aatgttcttt gatgaagagg tcgacagact ctttaagaaa atgtcgaaat
     2881 cgtttatgga ttttaatgac atctttgaag atgtcaaaag atccgacggt aagacgtttg
     2941 gaccatatta ttacggttat tcgataaccg ttggaccaga cggcaagccg cagataagag
     3001 agtatggaaa cgtcaagcca agcttgcttc caagctcaga ggtaagagag ccacttgtcg
     3061 atacgttagt tgacgacaag gaaaacactc tcaagctcgt agcagagatg cctggagttg
     3121 agaaaaaaga catcaaggtt gccgtagaag gaaatctagt caatattgat gcggaacgcg
     3181 gtgacaaaaa ataccaaacc aaagttccaa ttaaacacaa agtagatgtt gactctgtca
     3241 aagcatccta tacaaatggg attttggaag tacagttcaa gctaaagaag gaagacaagc
     3301 caaagggcaa gacagtggag gttaactaaa cctctttttt tatgggtgat aaaatgagcg
     3361 aaattatact tagaattaat gaggtttctc agcgacatgt aggaaagggt atagcggtaa
     3421 ttgatcccaa agtagtcaag gaaaacaact ggcagacagg acaaattgtg gaattacttg
     3481 caaacaagaa gagtcatgtg aagatttggc ccggatcaac ccaagattat ggttctggaa
     3541 taattagaat tgacgggttg acaagacata atatcggtgc cggaattggc gaaaaggttt
     3601 ctgtaaagct ggttaatgta gtagaagctg atcagattgt actttctcca attgaaaaga
     3661 tgtcagtgga aggattgcaa gaatacatgt caactttgta tgaaggtcat gtctttacaa
     3721 ctggcgatac aataatggtt aatacacatc ttggtggaaa agcacagcta atcgttacct
     3781 caacgacacc tgcaaaacct gtaattgtta gcccaaagac cacattcaag cttggagaca
     3841 ttactaaagc agtagataac tccgttccaa gaatcacgta tgacgatcta ggtggcctca
     3901 aaaaggaggt tcaaaagatt cgtgaaatgg tagaattgcc tatgcgccat cctgagcttt
     3961 tcgacaagct tggaatcgag gctccaaaag gcgtgctctt atacgggtca ccaggaactg
     4021 gaaagaccct acttgcaaag gcagtagcag gcgagacaaa tgcgcatttt actgcaataa
     4081 gtggtcctga aatcatgggc aagtattatg gcgaatccga ggaaagacta cgttcaatct
     4141 tcaaacaagc agaagaaaat acgccaagta ttgtcttcat tgatgagata gattccattg
     4201 cgccaaaacg tgacgaagtg acaggtgagg tggaaaaacg aatcgtctct cagcttctca
     4261 cattaatgga tggaatgaaa tcgagaggca aagtagtagt gattgcggca acaaataggc
     4321 cagactctat tgatccagcc ctcagaagac ctggaagatt tgatagagaa atagaaattg
     4381 gaattccaga cgaaggtgga agactagaga tattaaacat tcatactcgt ggaatgccaa
     4441 tcgacgaaaa agtaaaccta aaacaaattg ccaaagtaac acatggattt gttggagctg
     4501 atctagagtc acttgccaaa gaagctgcaa taagttcatt acgaaagata ctgcctgaaa
     4561 tcgatcttga ccaagacaag atttcggctg aaattttaca aaagatcaga attacagatg
     4621 atgacttcaa agacgcatta aaagaagtaa ggccatctgc tctaagagaa gtcctagtcc
     4681 aaataccaga tgtaagctgg gatgatgtag gaggattaga gtcgctcaaa gaggaattac
     4741 gtgaaacaat agaatggcca ctaaagcaca aagaggcttt tgagtatgtc aatgtttctg
     4801 caccaaaagg aattttgtta cacgggtcac cgggaaccgg aaagaccctt atcgcaaagg
     4861 cagtagcaaa gatgaccgaa tcgaatttta ttagtatcaa aggaccagag ctactctcaa
     4921 aatgggttgg cgaatcagaa aaaggagtaa gagagatctt tagaaaggct cgacaggcag
     4981 caccatgcat aatattcttt gacgaagtcg atgcacttgt tccacgaaga ggtggtcacg
     5041 attcatcaca tgtaacggaa agcgtagtat cgcaaatatt gacagaaatt gatgggttag
     5101 aagagctgaa caatgtgttg gtcattggtg caacaaatag acttgatatg gtagatccag
     5161 ctttgctaag acctggaaga tttgatagaa taattgaagt tccaaaacca gatgcacgtg
     5221 gacgaaaaca catctttgag attcacacaa aaaagaaacc acttgattct gatgttaaca
     5281 tgtcaagact agtacagatc acagagggtt acacgggagc tgagattgcg gcagtatgca
     5341 acagggcagc aattgctgca ttaaaacgac atgtatctgg ccaggtgcaa tcgctaaaag
     5401 acatcaagat aactcagaag gacctaatag aagcaacaag caaagtacga gtacctacaa
     5461 caatgagctc aacggctaat ccaatagcct aaattttttt tgtttaaaat ttatttttag
     5521 ttgcacttgt attacggcaa tttagaccga aaaagtcgaa tcagatttta tagatttaga
     5581 aatagtttac actgagttat ttagtaaaaa gaaatttttg atgactggac ttagtcgata
     5641 agttcagtcc agcctttaac gaagactgaa tttttgtata gaggaactgg ttgacctact
     5701 gtgaagtcca ttggtctcat ccaaaagatt gcctttgttg ggcatacacc gatgcatgca
     5761 ccgtcagaaa tgcatctttc tgggtaaaag acgaatgcct ttcctctctt ccagccttca
     5821 actggtttta ctctaagtac atctggtccc aaggttgtgc agatttctac gcataatgcg
     5881 catccaatgc acatctgttc accaatgtct ggtaaaattg ctactggcat ttttatcaac
     5941 ataacttgct tttggatctt aatataattt ccccaaaaac tgccaattat aacggcgtta
     6001 gtgaattttt aacgctgtta taaaattctg atcgcatttt tgaattttta caaaagattg
     6061 cttcctacaa caaatcgagc tcagaaatct ggagtgagga tttttccaaa ttatgatgat
     6121 cagctaatac aagctgcaaa tgtgaatttt gttttcttac tgaaatcaaa ttcatcttgt
     6181 caatttcaat tacatgtctt ccaattgcag acaatatcca tccttcatcc tttttgaatt
     6241 ttgaaatgct gcctacaaca atggcagagt ttgtcttttg tcctgtgcca acaagcaaca
     6301 tgataatatt gttgattatt cttccagaat ctttttgatt agttattgtt gtaaagaatc
     6361 catttaatga gtcaattatg actaggtgtt gtttttgtga gatctgatcc atgatgtcta
     6421 cacacagatt cttccatgtt tgttgtgttg gttgatgtag tatgacattt tgagattggg
     6481 ataatatctt tgcagcaaca taaccgctat acaataaatc aaaatcgaga tagagagttg
     6541 gcatgtctgt aattgaggtc aatttgtgaa aaaagtatgt ttttgttatt gcgtcctcaa
     6601 atgaaattga gcttaggcgt tcattgtaaa taattttctg gattttagct acgatgtcag
     6661 tcgaatcatt ctgttggttt ttatgcaaca actagtaatt ttttatcaga tataataatg
     6721 aatttagatc aagagctaat ttcccattct tttgtcaagt cagagcgtac ctatctcaac
     6781 aatgcgtcat cttctttgat tccgcttcaa acaatgaaag ccatgactga ctttaccatt
     6841 caatataatg aattaggtcc agattcgctt gattttgcgg ctcttttgaa agaaaaaacc
     6901 attcgactca gagaagctat ttccaaatta gtaaagtgta ggccagagga ggttgtcttt
     6961 acacaaagca caacagaagg aatcaatgcg gtttcaaacg gcatgtcatt tccaaaaaat
     7021 tccaacatga taatcagagg atcaactcaa gagcaccact ctaattattt tccttggttg
     7081 cgtctttcaa aaaatgtaga gcttaggagc ttggcaattg atgagagtgg gttttttgaa
     7141 atttctgagc ttgagaaatt agttgatgaa aacacgaaac tagttgctct tagtcatgcg
     7201 ctgtataata caggtgcagt tatgcccgtc acagaagtag gcaagatcct acgagaaaag
     7261 aacgtgccat attttttgga cgctgctcag acggttggtt gtataggaga gtttgatttt
     7321 tccaagatag atgccaattt tgtggctttt aatggctaca aatggctttg tggcccaatg
     7381 ggaattggga tttttgtctg caaaaaagat tcagcgggat tgcttgagcc gatgcaagtt
     7441 gcgggagaat cctcaatgct atatgataaa gaccagcttg cccacaagga cataccggac
     7501 aagtttcagg caagttttag aaattttgta gcagttgtag gactcgagtc ttctgttaat
     7561 tttttactcc attttggatt agaaaacatc agacacaaag taataagctt ggcaaatctg
     7621 ctaagagatg aactggcaaa aattcctgaa gtgacattgt atggtcctgc tgaccaacaa
     7681 aaaagaacaa gcatagtctc atttactgtt agtaaaaaaa gcccacagga agttgtcgaa
     7741 tacctggaac aacaaagaat cattcttgcc cagcgcgaga tatcggatag aaaaattgtg
     7801 cgtgcttctc ctcacttttt taacactgaa aacgacgttc taaggactgt agaggctatc
     7861 aaaaagctat agttgttcct gctcgtctat taccatcata gtaagtgtag attgaacttt
     7921 atcgattttc cttattttgt tagtaattat gcttcgtaga tgatcggcat tttctgattc
     7981 tatctttacg actatatcgt aaataccata gacgccctgc acttcaaact tgagtccttt
     8041 ttccacatta aggatctcct ttatttttgt aacaatttct tggtctgaac ccaaatcgga
     8101 attcagaaga atgaatgcag ttggcattgt agaatgtatt ctggatggga tctatttaat
     8161 atctttgatc ataagaaaat agaagtgatg tcgtgataat tttgaggcct caagatctga
     8221 cattaactgt ttcagaaaaa attccaacgt ttcctggctc ccctcagccg cactttattg
     8281 agtgggccga gctaaaaaaa gatcattata accttgaaat gctttttttg agctcacata
     8341 caggcacgca tgtagatgct ccttatcatt ttgtaaagaa tggcaaaaaa attcatcaaa
     8401 ttgccccacg gcgattctta ggtgacgcaa ttttaattaa aattagtaca agaccaaatt
     8461 atggaattac aaaatctgat attttaaaat ttgaaaaaaa gaatggaaaa atcccaaatg
     8521 gttcaactgt ggtgtttgct actggatgga ataatagttt gcaacgaaag aactttttta
     8581 aagaaaaccc gggccttgcc gcctcagctg caaaatatct ggcctcaaaa aagaccaaca
     8641 tggttggaat tgattcgcca agtattgatg ttggaaaaga ttcatctttt tccgctcatc
     8701 acatactgtt agaaaatgac gtgcttattt tggaaaatct ttgtaatcta aataaaatta
     8761 aatcaacaag gttcaaactt gttgccttac cactaaagct tcaaaacgca acaggttcac
     8821 ctgttcgtgc cgtagccttc tagattacaa attggaatct gtttttcttg tattttttgt
     8881 ctagcgggcc gatgattgga atctgctttc cacaaaactt gcacttgttg tttgcatcaa
     8941 ggttccatgc tgtgatatcg aacccatatc tgccaactgc aatgtttttg cactcagggc
     9001 agtatgtgtg ttcatatgga tggccaggga cgtttcctag gtacacatac tgcaatcctt
     9061 ccttttttgc aacctcgtag tgtttttcaa gagttttggt tggagtaggt ggaaattcca
     9121 tcatcttgtg atctggatga aatctcaaaa aatggattgg catctccggc ccaaactcat
     9181 catacaaaaa tttagctagt ttttttgtat attccaaatt gtcgccaacc tctggcacga
     9241 tcaagtctgt gatctcaacg tgaatcttgg ttttgtcacg aatctccaat aatgtgtcaa
     9301 agattggttt tggatcagga acaccaatgt atcttctagt aaagtcgggc tctgcacttc
     9361 ccttaaaatc aactgtgatg caatccaaaa attcgtccat catttttaca gtttgtggag
     9421 tatcatatcc gttagagaca aagatgttaa agagtccctt tttgtgtgct tcaattccac
     9481 aatctcgcgc aaactcgata aaaattgagg gttgattgta cgtgtatgcg atcccgtccg
     9541 atccattatc aactgccatt tgagcaactt gttcaggtgt catgtcagct ccttctactt
     9601 ttcttctctg tgatatgtca tagttttgac agaacttaca aagccaatta cagccagtcg
     9661 tagcaataga aaagattcta gtccctggac gatagtgcac aactggtttt ttttcgattg
     9721 gatctacatg tcctgtgata actttgccat agacgaaaag gtcaagctta ccaccttcat
     9781 ttcccctgat tccacatagg ccgatttggc catcctttat ctcacaatat ctagcacagg
     9841 cagtacaacg aacgcggcta tctggtagtt tttcatacaa aattgcttct ttattcatgc
     9901 caccatctta cgttaatttg caatataagc gatagctcat aatctaaata tctaagattt
     9961 taaaaatagt aaatgagtaa accaggaaac ttttggcgta aaattcatgg aagggtcaca
    10021 aaaaggccta ctaatttttc atggcttcta caggacaagc ttgcaggatc tggcatgcca
    10081 acgacttttg aagagatcaa ctgggttcga aagcaaggag tcaaatcaat tatcacaatg
    10141 acggaacggg gtttgccaga ttcttgggta gaaggactag aatatctaca cattccaaca
    10201 gaagacctct cagccccgga catagacaaa attgatgcta cagttgattt catacatgag
    10261 caaattaaaa ataaagaccc agtaatggtg cactgtgctg cgggaattgg caggactggt
    10321 acaatacttg ccagttacct tatcaagtat caaaatctta cagcaaagga agcaatcaaa
    10381 aagattcgac aggaaagacc tggctcaatc cagtcagaat cacaagaaat ggcagttact
    10441 ttttatgaaa aatttctaaa atcaaagtga gccaatcacc aaagatttgt gtaaaatgta
    10501 agaattattc aggaattgag gtggtttcta ttttgccatc gactagtttg acaaagagaa
    10561 atctattgtc cttaaaaatt agtgtcacgc cgccttctgt gtccggttct tctacctcaa
    10621 gaactggttg gcctaagagc ccgtcatatt ttgccatgaa tcttttttaa aaaactagct
    10681 tatatcccta cttgattgtt atctcgattt cttctgcatt agattttacg tcgtctggat
    10741 cactaaagtg gtgtttttgc cacgaggcgt agatttcggc accaattttg tgttttccgc
    10801 ttccaagctc agatgcctta aaggtgagtt tttcatcaaa atcaaacaat tctcccctta
    10861 cttcatcctc cgtgagcctg atgattggct caaagttttt tgccacctgc acccagaccc
    10921 gtttttcctt cattggattt acaagttttg ggttgcgagt ccaaaaaatc gaggcctttc
    10981 tgtacgtatc cactgatttt agttcacgtt ttctaaagcc tccagataca agttttattc
    11041 catattttag cttaaacaga ttatcaaatt tgttataaga atcagtccag tttttttcgt
    11101 tgaatgcctc acgcaaggcg ccctgaattg aaaacctgat gccgatcaca atatcatcgc
    11161 tattagaata ctcatctttg tcgttggtga ggcttacttc tgagatgaat gtcttttcgc
    11221 ttcccatatt gcacaatgat ttatatccgc aataagtggt tttccgacag aatgaatgcc
    11281 caaatagtca agtcctccac aaaggaaaca aatctttcga tcagagattc tgacattggc
    11341 acgctgtata ttgtccagca cgagctgcta aaggatcaac aggtagaatt tgcaggggta
    11401 attctcagac atcctctaac aagcgagtac tctatgcgtg taaattcttc aaagggcaat
    11461 ccattaaagg aaatcctaaa ggcaacagat tcagcaatcg agtttaccaa cggcctgaaa
    11521 cagacaattc actcaaaatt caagggtgtt tagttgcaat tttgctccac atgtggcgtg
    11581 cgtctaaaga aaggtacttg ccaaaaatgt ggttattctg aaggcgagtc aaaacaagaa
    11641 ccaaaaaagc tgactgaaca atcacaatca gagctgacaa ttcttgaaga aaccgatggg
    11701 aaagaagctt tgtcatcaat cacaattgat tgtgaaaaat gcggcaatac tgaagctgtt
    11761 tggtggatgc ttcagacaag aagtgcagat gaaccaacaa ctcagtttta tcgttgtaca
    11821 aaatgtaatc acacttggcg aaactacgcg tgaactatta ttggtagata ccatgacttt
    11881 agattaatag aaattttata gtgataacgt ttaactggca ccatattaac accaaattta
    11941 gctttggtat tttcagcaaa aacaagtggt tcagatgaat ggaaggcaat actttcagcc
    12001 atatctactc tggtagaaga ggcgaccttt gaggccactg cagaggggat agcttttcgt
    12061 ggaatggatc cctcgcatgt tgcactaatt gatatttctt ggccaaactc tgcctttgag
    12121 aaatacgagt gcgatggcga cataaaattt ggtgtaagaa ttgatgaatt ctcaaagctg
    12181 atcaagcgtg cagaaaaaag cgacggaatt gagatcagtc tttcagaaaa catgcttctc
    12241 atatcaattg gaaagaacaa gcagtacaag atgagattaa ttgaaagctc agcaactgat
    12301 acaccgctgc caaaaatccc atacgataca aaaattggtc ttgcaaccgt tttgtttgac
    12361 aaaattttgg gcgatgttca agttgtttct gattatttga ccattaaaac aacctcgaca
    12421 aaatccgagt tttccggcaa gggagacgct ggagaggttc tcatatcact tcaaaaacaa
    12481 cagggcgaac tatccgagat ttcagtcaaa gaggagagtg tgggaaccta cagccttgaa
    12541 tatctaaatc caattgtcaa agcagtgggc tcagcctcag agttgattat ttgtgaatat
    12601 tcgtctgcta agcctcttcg aatcgagttt aaggtagcaa acatgggtag aatccacttt
    12661 tacctagcac cgcgagttga aagttaaaaa gcatttatgg tttaagtcca aatgacatct
    12721 aagttgagac ttgttctaaa atttggtggc acgtcacttt cttctgcaaa acagattaag
    12781 gcagttgcaa aattcgttca taacgtttcc aaaaaaaatc aggttactgt ggtatgttct
    12841 gcaataaatg acgtcacaga ccagctaata cagatatctg aatttattca gaaaaggaac
    12901 aaagccggag caagttcgac cctagcaaag ctcaaaaaac agcaccaact gattgcaaaa
    12961 gattccatat caaacgctaa agtaagaaaa aaactaggcg agaaattaaa tgccaatctt
    13021 gcagaacttg aaggactact tcatggcatg atgttactag gagaagtcac ccctcgttct
    13081 ttggattatt tgatatcatt tggtgaaaga ctatcagttg gaattgtttc atatgtcttg
    13141 caagacctaa aatcaaagtc gattgatctg actggaaagg atgttggaat tatcactgat
    13201 tcaaactttg gctcatcgaa gccattgatg gatacaacac gacttagagt atctgctaac
    13261 ctagaaccct tactttcaaa gaaaataatt ccagtaatcg gaggttttgc tggggccgat
    13321 caacacgggc acattacaac ttttggacgt ggtggttctg attacaccgc tacaataatt
    13381 gcttcttgca tcaaagcaga cgaggtttgg ttgatgagtg atgttgatgg tttgatgact
    13441 gcagatccaa aaattgtcaa aaatgccaag gtgatccccg aagtatcata tgttgaagca
    13501 atggagatgg cactttttgg agcaaaacaa atccatccac gctcctttga gccgcttttg
    13561 acaaagaaaa tcccgttacg aatccgaagc accttcaatg tcaacaatcc tggaacgctc
    13621 gttactgcta cgcctgatga gtctacaaac aagacagtca agtgcgtcag tgtgattcgc
    13681 cacaacggct taatcgacat gaggggcggg agtatggtcg gcgcaccagg aacggcagct
    13741 accatatttt ctgctttggc aaaggcagga gtcaacatca tgatgatatc gcagagtcct
    13801 tcagaatcca gcatctcagt tgtagtaaag aaaaacgatt tggatagggc agtcaacacg
    13861 atggagatga atttgcttgg taagataata aagaaaattg acgtgactgc cgatgttgcc
    13921 ataatcgctc tgatcggatc tggtatgcga ggaacggttg gtgtggcctc aaaggtcttt
    13981 ggcgccacag caagaaagaa agtaaatgta gttatgattg cgcagggttc atctgaactc
    14041 aacttggcct ttgttgtcaa agacagtgat tgtgcagttg tagtacaagc actacatgag
    14101 gagttcaagc ttgcagagca aatcagtcaa tagttgaaaa acttaaacat gtttggaagc
    14161 cacgacagat gttgagaaaa ctagcaatca tttctgtaat tgtatcagta acactagttc
    14221 ttacagcatt gtcaatgctt tctgacgagc ttgcagttgc aaaaccacaa agtaaagttc
    14281 attttacaaa aaccataaca tcgtcgcagg atcccggagt aggacagggt agttacgagt
    14341 ttgcactcgt tctttcacca aaccaaggaa gtctctatac tggatcactt acatacacag
    14401 caagcgaggc agtcgaagtt gttgtattgc atgaacttga taaaactgac gatggtgggc
    14461 agccgacttg gacagtagat ggcaacacca tttatgcaat gagtgtaatt gagccgtcag
    14521 aggcacgctc atttgatttt actggtgcgg ctattgcatt tagaagtcag aatccattta
    14581 tcgttactgc cagcgttgat ggttggatca gaggccaacc ggtagaatta gttatacaaa
    14641 aattagagat caaggaaaaa tcactttctc ttccagacgc acgtgtccca gtaacaattc
    14701 caatgaaggt tggatttttt ggcaaagatt ctgtttacta cattctcaca gactctagca
    14761 acaaaacgtt ggcagaaaag atcacagaaa aacaaggctg gccagtccaa tttgctccca
    14821 agcttaggtg gtcaccagcc tcctcgcaag ataccatcta tgtattcaca aatggtgtca
    14881 ctggagacgg aatccatggt ttccaaggag aggtcttctc aagcacccca gctcaaactg
    14941 agtcatacag tcccctcaga acattagtta gtgtatcctg gaaagagggg caaaatccac
    15001 agatactgga tactgccgaa gacatactca aggccgaaaa ggacagcagg attaagcttg
    15061 taaggaccaa tgttacaata aatgcgccgc aagttgcatg gcctggaggt cagctgtttg
    15121 ttagaaatga aacgacccta tcaaatgaga cttttgaaaa aggccaagta attggaatag
    15181 acaaagatgc aaaaaaagtc acctttgttg cacaccgagg ttggggtttt gatggtagaa
    15241 ccatttacta tataatgcct gatgcaacac caaagggccc tgcggatctc atgggtattc
    15301 cagtctcacc aaaactcgca aatgtgttat caggtgttgt atttgcagac atgtatcaat
    15361 tcaaaaacgg attcaaaggt ccaggccagc ttggatttca ggcaagcatc atttcatcaa
    15421 agatggatga aagctatgtt ccaatttgtc gtgtctccat agtagagtgg aaggagccag
    15481 attctgcatt agttcttgaa acgatttctg atattaataa gaaaaaatca gatggtagta
    15541 tctttgtaac actcgcaagg ccactaagtg acgatcatgt ggtaaattgc cctctggttg
    15601 aactcacaaa tttatccaat aagggataaa ttatcttaca aaagactaaa ggatcacttg
    15661 tcaggaaaac tctacatcgt tggtgtcggc ccaggaagcc acgatcatat gacatttagg
    15721 gcaaagcaag taatcgaaga aagcgacacc atagtaggat atgataccta tgttagttta
    15781 gttgaagatt tgatcaaggg aaaggacgtt tatcgctacg caatgaccca ggaagtagag
    15841 cgtgcaaagc agtgtattga tcttgcgatg gaaggcaaaa tagtttcttt ggtctcaagc
    15901 ggagatccgg ggatttacgg catggctggt ttaatttatg agacattagc agagtcagga
    15961 tgggatccgg aatctggcct tgaagtcgaa attgttcccg gagtttctgc gcttaattcc
    16021 tgcgcatctt tggttggctc gccactaatg actgattttg ctgtagtcag catgagtgat
    16081 cttttggttc cttgggagat aataacaaaa agagtcgagg cagcagccca aggagactat
    16141 gtaattgtaa tttacaatcc gtcaagcaaa aaacggattc accagctcca agatacaagg
    16201 aaaattttgc tgaaataccg caagccgaca acccccgtcg caataataaa gggcgcatat
    16261 cgcgactctc aggtagttgt cataactgat cttgacaata tggaaaaaca tgcagacaag
    16321 cttgggatga taagcaccgt gataattgga aattcatcaa catataattt taaaaatttg
    16381 atgatcaatc cacgaggata tacatcaaaa tacaatttgc aaacaaacta agttctaaat
    16441 gctatttttt attgtgttat acagctcttc atctaaggca atcttgtcac ggattggtgc
    16501 tatgattttt accagatggt ttgctactgt ctgcttgaga tcagacggat gtagttttgc
    16561 cgcgccaaag tctgattcta attgtgcata actagcatat gtaacatttc ctccaaattt
    16621 ttctggtctt tctactgtaa tttcagaaaa ttcatgaaag atgatgtgtc gtgcaatctg
    16681 cagaattggg ttgtttaggt tgcctacttc acaccagcct tttttgattt ttgagcgaat
    16741 ctcgtcatcg gaatcatgaa tgaatacacc tgaacttggt ttggatttgc tcatctttgt
    16801 tcctgcgtca tcctcaacta caggttcaga taggccagga agaaggctgt gatgtacagc
    16861 aactggtacc ttccatttca tttttggaaa aatctcgcgc acaagcatgt gaattttgcg
    16921 ttgatccatt ccagcatgtg caatatcaac atcaatggta tgaatatcta ctgcctgcat
    16981 cggtggatac aaaagttttg atagatcaat tttttcctca ttttctgagc ggcccatgat
    17041 tgtcagtgtc cgcattgttc gggcaagcga catgtgcttt gtaaataaga ttaggttgct
    17101 ccaatattcc ttgtgtgaat cataaagctc tgttcctttg ataatgttga cgcctggaca
    17161 tacaagcttg aatgcttttt catagtaatt tgagacctta gatatgatat cccagtttcc
    17221 ttcaagcttt tcgttaatta tggtgtgcca gtctgccaaa aagacattgc attgtacgcc
    17281 agcttttatg aaatcattaa ttttgaatcc tgtacttatt agactcccta gatggagaaa
    17341 tccggaaatc tccagtccaa tgtagtgttt tggttttgag tttgtttcaa aaagatttgt
    17401 tagttcttct tttgttacta tttcttcggt tggcggtttt gatattagtt cgattttttg
    17461 tgcaacatcc attctagttg acttttactt gaaactctat aaagatagtg aaaaaacaaa
    17521 tatgaaaact gcgtttattt tgaacacata tttttgaatg cttcttgtcc tgctttttgg
    17581 agttcttctg cagacatgtc ttttttgtga gtcgcaatgc cccagatatg tccaaaagga
    17641 tcttgcacgt tgccaaacct atcgccccaa aaagcatcca tcataggcat aattggcttt
    17701 gcgcctgccg tgactgcctg attgaatact gcatctgcat cttcgacata catgtaaatg
    17761 gccattcctg agccaccaag agattgtgga gaaagacagt tcatctgtgg catttcatca
    17821 cttaacatta cgattgagtt tcctatttta atttctgcgt gcattatgct atttccatcc
    17881 ggtccaggaa atctgtaaag ttcttgtgct ccaaacgcct ttttgtaaaa ctcgatcgca
    17941 tttgcagcac cctttactat caaagagata gtgaccgtgc taaaaccatc tggaattggt
    18001 tttgtcatgg agataacttg tggatgttta ttttaaacat tattccaatt taattggttg
    18061 atctacatag attatggatt tagatcgtct agtgactttc gtggtctggt gctaagatag
    18121 aatgagtgtg cactgatcaa aaatcctgca aggaatattt ttgttgcaaa aataagattc
    18181 catggtcttt gtggatccgg atatgctatt gtaagcgagt caatgtctgg cacttttcca
    18241 ttctcaattc cagcagagat ctgtgcctca agaactgtaa aattgtacaa aacctcgtaa
    18301 aggcaaatca cacaaaaacc aagaagcatt agttgtaaca acgacatctt ccaggaataa
    18361 agctgggttg ttttcttcca accaattctt gaaacacaat accagccaat tatgattgca
    18421 aaaaataacc atgtaatcag tttggcaaaa tgcggaaacg gaaattccgt ttcgacaaga
    18481 acttcgccca tcacatatgt gccattttct tgccattctt gcatcattgc ataaattgca
    18541 tagaaagtaa tcgaggtcat cataatagca cagatataga aaatatacaa gaaaaccctg
    18601 tagccttttt cgtcttcctg caaatggtgt actttcatgt atcgaccgct caacaaagta
    18661 gctataaaaa tcataaagca ttgatgagat ttattagagt ttgacaaaaa gacaaggcat
    18721 gaaggttcct gtgaatatac ccttccttgg aaaagaagag attgcagagg taaattctgt
    18781 tcttaaaggt ggcacgttga catcttcggc tagtcttgga ggtaaaaatg tgcaagggtt
    18841 tgagagactt gtttgttctt ttatcaaatc aaaatatgcc attgccgtca attctggaac
    18901 tgctgcgctc caagcagcct tgtacgcgtt tggtatcaaa tcaggcgatg aggttctttt
    18961 gccttcattt acgtttgttg caaccgcaaa tgccgttgtt tctgttggtg caaaaccagt
    19021 ttttgttgat atactaaaag aaaattacac tatgagtcct tctgatctga aaaaaaagat
    19081 cactaaaaga tcaaaggcga taattccagt tcatttgtat ggtaacgtag catacattga
    19141 cgaaatatcg gagattgcaa aaaaacacag tttggacgtc attgaggatg ctgcacaatc
    19201 catgggttca agctacaaag gaaaacaaac tggaacgttt tctgagattg gttgttttag
    19261 tctttatgct gcaaaggtaa tgacgtcagg tgagggtggg gtagtggtaa caagtaacaa
    19321 aaaactctgg gaaaagcttt tgatgataag aaaccatggc atggttcatg ggtatgatac
    19381 acgcgtgctt ggtttgaatc tgagattgcc tgagctcaac gccgcaatag ccaaagtaca
    19441 gatgaagaag cttcccaagt ttttacaaaa gcgtcaaaag aacgcaaaaa ttctcaccga
    19501 gctacttact ggtctggaca taggtcttcc gcatcaaaga aaatatgaaa aaacaaactg
    19561 gtatctgtac acgattgcca caaaggctag aaacaagata atgaaggaac tcaacgcaaa
    19621 aggaattggt gcgacggttt actacgaaac gcctgtgcac aagactccct tttacaataa
    19681 gaaaatcagg ctggaaaata cagagtgggc tgcaagccat gtgctttcat taccagtcca
    19741 accaatggtt acacagcacg atttggagct gactgcaaaa accattcgca gtgtggttac
    19801 ttgaactcct tagtagaact tgccggccag ctttgcaaaa tactcctgcc aatagatgaa
    19861 gaggtgtttt atggcgaccc gtcatcaaat gttgccatat gtacactctc aagcatgagt
    19921 ctcttaaagg aaatatcaaa ttctgattta atgactgaga ttactcttgt aggtaggcta
    19981 ctatcagaaa acaaaggaat tgatgcatta atccgccatg ttagcaaaca tccaactctt
    20041 gacacaataa ttatttgtgg gatggaagta tcaggccata gggcaggtca tgccctcttt
    20101 tgtgtacaca agtatggtac agatgagcag aacagaataa ttaattctgt tagtccagac
    20161 ccggtacttt ctgtttccaa gtccgaaata caacaatttc aaagacaaat tacactaatt
    20221 aacaaaattg gccaaacaag tctagaagag atcaggtctg ttatgagctc tcagtagcca
    20281 tttctttgcc ggtctctgtt aatttcgttt tttcttttgt attctgctat gatatcttct
    20341 ggggtgaggt ttagctcaag tgatgcctgc actacaaaat gccagacatc aattagctct
    20401 tcttttgcgt cagctatgtt aaacggtatt ggttttttcc accacttcca gtttgttgtt
    20461 ctctgaagtt ctactgcctc atgaattatt gcagtgcata aagcagagat tcgtccttct
    20521 acatcttttg gatatctatc aaggttcatc atttctgcga gacctttttg cagggagaaa
    20581 attaattcta gtttatccat gaacagattc cgaatcggtt ttcttaaact tttcagaaac
    20641 caatcatttt ttggtattgt ttgattctat tttcgatctt tgtcttattg atttgaagca
    20701 gaccgtctac accatatcgt tcaactgcaa atgagccaag aacatttcca taaataatgg
    20761 actttttgat ctcagatagg cttgtggttt ttttgctcgc taaatagcca atcatggctc
    20821 cagcaaacga gtcacctgcg cctgtcggat ccacaatgtc ttccagagaa aacgcaaccg
    20881 atgggaagat cacgtcttcg acgaaaagaa gtgagccatg ttcacctttt ttgattatga
    20941 cgtattttgt accccaaccc atcatttttt ttgcgcactt gatcaggttg tgctcttttg
    21001 taaggagttt tgcctcttca tcatttatga caactgcatc tactgccttt atcattttga
    21061 taactgaggg gcgttttgtt gcgatccaat attcaatggt atcgcacatc gaaaatttga
    21121 tgttatcaaa ttctttgatt agtgaggaat tctggtccgg atcattgttt gcaagatata
    21181 caaattcaga ttttttgtat tcgtccggta ctgttggctt gaagctccca agaacattta
    21241 gatctgtctt gtttgtagtc cttttactaa gggtcaggtc atagctgcca tcgtatcgaa
    21301 aggtttttcc ttttttgact gcaaaaccac tcaaatcaag atattttgag agtgtgtcat
    21361 ggtatttttt tggaaagtcg gtgccaacta ctgcaattag accagtattg acaaaaaagc
    21421 tagcagaaat gcctgcaaaa gttgcagccc ctcctaagac ctccttgaga atcttcgttg
    21481 gagtcctaat tgtgtctagg gcagttgaac caaaaacagt aagcattatc tgcaaaatta
    21541 tgtgtcgtgt ataaatttgc tagtctggct ctctgttgga tagtatgtga atggaatctc
    21601 aattgatgca aagagtagct ggtattttgt cacgccaatg atttttactt gtgggttctc
    21661 cgagtttgcg ctatgatcct taaggaacgt accgcttgtt gttgcttttc caagcgcagg
    21721 aagcaggaac ggatctaatt ctcccccacc aagcgtatca tcatctgcag ctatggtaaa
    21781 ttcagtcttg cctgcattaa gaaacaacag tgatgggttg ctgaattcaa gctccaagtt
    21841 atacagggag ccctgctctg ttttttggat taattcgctg tctgtgatca ctacggtaat
    21901 ttgatgagct gatgcgtatt gcgtgaagcc aaataaaatt accccaatta ttgctgctgc
    21961 tattatagtt gccttcttct ttttagtcac gctttaacat atcaaatctg cttttgtgtg
    22021 aggaggcgaa agattttctg ataatgtgtg taaaaatttt gatccgttct ttaagctact
    22081 tagagctagt agaataaatg aaaatctctt acttttagta taaatggcaa aaattcgact
    22141 cagattcggc gaaaatgagg tagaaataga gtcacgtgac ttttacatag ataacgatac
    22201 cgctggacag gtaatttcag aacttgcacg taatcttgag aacaaggcaa gaataatcac
    22261 agataacgaa atttcattag aaccaccaca gcaaatcagg cagacatatc aagcaaatgt
    22321 cgattatttg agaatgctaa aagacgctga ggttcacgag ccagagtttt ctccgccaat
    22381 gccagtctca tcagatgaga tacaatcaaa gatcgagtcg ctagaaaacg attcgttctt
    22441 tttaaaaccg cgaaccgtat cggaaactgt cgagcagcta agagaatatg gatggcttgc
    22501 aagtccgctt gatgtatcaa agatacttgc aaagatggct tttcacagag agctggcaaa
    22561 agattcccaa gaaaataaga cctattattt caaaaaacag ataattgttt aactagacgt
    22621 tgtatttgca ggtaatgcat ttagaaaata ctgttccttc caaatgatca aagtgtgtgt
    22681 tgcatttttt acaggtgtgg tgcatatcaa aataaccatc ggcttcggct tgccctacct
    22741 gaaatgattc aaggtctgta ctcttgcatt ttatacaatg acacccacag tcgcactgca
    22801 tgtggtgaca gggttctttg tgttaataat ttatttcacc tcagtttgta aaggagtgtt
    22861 ggcaaaaaag cggaccatac caattgagat tgatgaccat tttaagatat atggtaagga
    22921 accttgggaa gtcgattatg gggaaaagtg cccaatatgc aatgttagaa tagatgagta
    22981 tggtttttgt tcctgcggtt ctagcggaga ttaaagttgt tagtataacg ggcagcaaag
    23041 atcatggcag tagccaatat caagaccgct gcaaatggga attctggaac tgccgatacc
    23101 ccaatgattt caacagtacc cgtttcgttg gtttttatgt taagccatac gtttttttca
    23161 tctgaataaa actcgtgttt tagcattttg ttttcgttta agaacacttc gtatggattc
    23221 caaagtaatt ctaaggggat gattaaggtg atgtattggt ttgcatcact gacgtcaaag
    23281 ctgatttttt tgtttggttg atcaaattca aatgaagaga tgtctgtatt tgtaattatt
    23341 ctgacatcaa attttttatc ttcccattgt acctgttcta ctgtttctgt tttatcaagt
    23401 tgatattcta gtattacctg acacccatga caccgaatgc cgtcaagatc actcaagttg
    23461 atcggatttc catttacgaa tacgaggtct gcttttgtag gcaaataaaa tgatgtgctt
    23521 gcaagataat gatagtccca agtcatcatt ccattttttt ctgatattac atcagccaag
    23581 tcatactcaa cattgacttt tttctttgtt ggaagaatca aaaatgcagt tcgatcacca
    23641 cctatttctc cgtattccac agaatcaccg tcttggtcag ttatttgcaa attaataaag
    23701 tcattactaa tgacgctcag ctgcctagag ttgccacttg gttctacaat atgaactaca
    23761 tgtgcactcg agtcctcgtt tattgtgatt tgaactgatt gtactgcagg ttgaccaaaa
    23821 tttacttctt gagcaaagac tgtacttgtt gtagagaata atgttacaat aattaatcct
    23881 aaaataattt ttttcaaaat taccgcctat tcagatactg aaacctttaa cattctgaca
    23941 tcttctagtg gacagtcgtt tttgtctcgt tgttggctga ctatggtatc tgcaatattg
    24001 attccctctg ttacctcgcc aaatacagtg tattgcctat caaggaatgt gctatcacta
    24061 gttacaatga aaaattgtga gcctgcgctg tcggggtctg tggcccttgc cattgagact
    24121 attccacgta ggtgtgactt tgagttaaat tctgccttta ctgtgtagcc tggaccacca
    24181 aggccccact tggctttgtc ttgtgttttg gtatttggat cccccccctg tatcataaag
    24241 cccggtatga ccctatgaaa tagtgtaccg tcataaaacc ccgattgcac caagttcata
    24301 aaatttctga cagtctctgg cgcaacatca ggtagtggtt taaatataat cttgccaaac
    24361 ttggtttcaa tagttacatt taccaattat tggcaaaatc gtttcttatc ataagagtct
    24421 tactattctc agataaatta ttttgaaatt agattgtaat tattacatag ttttcagcca
    24481 tgctgcgttt ctataatgta ctttaaaatt tattatgaga aaagagttgc tctaaaattt
    24541 cttgttataa taattttttg tagttcatac ggaacagcat tgttttgttg gtaaagttat
    24601 agtcttatat agatcgagaa aaattacagg ttgtgaatcg cacaaatcaa agcaccgtca
    24661 taaaacaagc aatcaattct tatcttgaaa aggggctaac tgacaaacaa gacatttact
    24721 ctaaagttgt tgaggaactt ggcgttccaa gaccaacagt gcgaagagta gcacgagatc
    24781 tcagaaatga gttgctggtg aaaattaaaa ttttacaatc agagacccca caggcagaat
    24841 catctgcgca agaaaaagac gaataaattt cgtttatctt ttcttttatc ttttctttta
    24901 ttcctagttg agaactagca ttactgttat attcgacttt tttggcttta aaacatgggt
    24961 tatttgcgaa accagctggc aacggtagtg gtttctgctt ttgctgcgtt tttgacaggc
    25021 atgtactttg ttctgccccc cattttgaac ttctttatgc tggtggtagt aatagtaact
    25081 tggtttttgg ctgttatgtg ttggattgta caaaaaggta cagattactc acatcgtcac
    25141 agccaacaac atggctacag tgaaaaaaaa agctcagcta gtctagtcac atctacttat
    25201 acaaagacgg tatctttgga tgagctgacc aagttcaaag agtcgtttac tgtagaactc
    25261 aacgagttaa aggacgaagt caagctcaaa gatggagaaa taagcaacct taggcagcaa
    25321 attgccaatc tccagacaca agttcaaatt gaggcactca aagtagaact tgcaaatctc
    25381 aaagttcttg cagcaaaaga aaagtcatca aaaagaaagt aatttactga caatgcttgc
    25441 aaccacccga tttgtgatgt tgcttgcaac tcggacagct tacagccccg ccgaaatggc
    25501 atttgcaatt acagccatca tttaaaatct ctttatccac caacaagaat aattgatacg
    25561 tcatcttaat atttgactgt taaagaaagg ctttaatggt cttaaaggcg gttatcgcaa
    25621 gtttggtttt tgcactagtg ttttcaacag tctccatggc atttgctgat aaggttcaaa
    25681 cacagactac cattactccg atggatgagt cgattggact agaaaagaca acagtcccgt
    25741 ttcatgcacc aacacaaaac aaacttccat ggggctttgt tgaagggaaa attgccaatc
    25801 cagtagaagg gtatccagtc ataatccaaa ttttcaagaa tggtgaagca atccactttg
    25861 cacagacaga tgtcaatgaa gatgggatgt atgaatacaa atttagagta agggatgtaa
    25921 caaacagccg ggtcacgacg atttttgaag gcgattatac tgtcaagatc ttcaaggtag
    25981 tgtatctaga caaagtcact actctagttt agaagcaaga agcatcgttt catcaactag
    26041 ttcttttagc cttgttttgc cattttcgtc tataattgcg tcatctttga ccatatccgc
    26101 agtcatgaat actgcttttg gatttgttat tacgcggaaa taactcataa gttgcgtaag
    26161 caaggtctgc acatcgataa atccgatatt tcccgaagcc agaatagcaa taccggcagt
    26221 tttacctgca gtgttcttgt agtttatgaa ttcaaacatg tttttgagag cagagctgaa
    26281 aaacgagtta taaattggcg ttccaaccaa ccacacgtca gcttctgtga tgtcttttgc
    26341 tgcctgcagg gttgtctgag agtaattttc gtttgggcca cggtaataat ctatgccacc
    26401 atctgagagg ttgataaatt ttgtatcaga gttctttgat tttgcatact cgtaaacaaa
    26461 tttcatcatt acttgcgtgt ttgcagtttt tcttggacta ccggatagta caacaatttt
    26521 cacaaacatt gcgtcgttta atcgctattt aaattaaaat aaaaaaacgg gcttggaggg
    26581 cttcgatccc tcgacctgca acttaggagg ctgccgcgct atccatgttt cgcgtcgtaa
    26641 tgactctgcg ctacaagccc ttgcaaaaat caactggcaa ttacttaaaa gcctaatcaa
    26701 ggtttgttcg aattaactcg tagatctgat tccaggtaga gagcctttca gtctgccccc
    26761 atttttcaaa gtaaactaca tcctttagct caagtcgagg tagccagttt gcagtctcca
    26821 agtcgggttt ttctgcaaat tcctctacat ggccaaggca aagatatgct actgggatta
    26881 tatggtcagg aagatcaagt gcttgtttta gagtatcatt tgaaagaatg cttacccatc
    26941 cgacaccgat ttcttctgaa cgtgctgcaa gccacaagtt ctgtacggca cagcaaacac
    27001 tatagatccc agtttcagga atgcttgatc ttcctatgac aaatggacca aatcttgaag
    27061 ggtcatatgt aacacagatg ttaacgtctg attctagaat tccttcgagt tttaatgaca
    27121 ggtacttttc ctttcttggc tcttctactt tttttgatga gtcctttctt tcctcttcaa
    27181 acgagttttt cactttattt tttgttatag gatcacgaat caaaatgaaa ttccatggtt
    27241 gtgagaaacc aaccgatggt gcatgatgtg ccgcatttag gattctggcc aaggcctcat
    27301 cagatattgg ttttgaatta aagtgagagc ggacatctct tcttgtgtaa attgccttgt
    27361 ataggccaga tttttcttgg tttgaaaatt tgtcgctcat gctaatctga cttgttgtga
    27421 ttgttgttaa agattaataa tgtagacatc aagtcttgta cttcattggg ccggtagtct
    27481 agtcggttag gatactgcct cgacacggca gtgatcgaga gttcgaatct ctctcggccc
    27541 acttttaatc atccttgttc cgaaaacaat tattgtgatg tctcaaattc tttttccacg
    27601 aggctccaag ctgactctac catcatcatg cctttagcgc taaacgttac tgattggccg
    27661 ttaactatta cgtcgtattc attagaattg tcacctgatt gtattaactg aaaggtgttt
    27721 ggtagttcta tttctgctcc tggataaaat gcagccctac ctggaagtgg aatgtcttca
    27781 gtagagtatt ggcctttgcc aataggttta ccgtttgcga atagctcgta atcaatgatt
    27841 gataccgtga atgttttctc gctaggattt cttatcagaa agattacttc cagcgtagca
    27901 cggttttcta tattgtttac attttgtact ttcacagttg taagctgaat ttctgccatg
    27961 tcgagttgcg gattatcaag gcttgcgtac cacgtaaatc ctccaagcat agctaataac
    28021 gctcctattg ccgcataaag tatgcctttt cctctaatca attgttgttt tccaattatt
    28081 acaactaaaa aacgttccag tcacatagct aatatttgaa caagaattcg atagtttgat
    28141 ggcactagaa caagctatca tcacgtggat tcacttgatt tcttcagcta tttgggttgg
    28201 cggatctttg tttattgcag tagtgtttgc gccagttctc aaaacaatgt cgtcatcagt
    28261 agaagaaaga ctgcaaatca tggtaaaggt tgggagaaaa tttaacaaaa tcgccatgcc
    28321 atcactgatg attttaattg ggactggaat ttttaactcg caccagatct taagtaggcc
    28381 ggactttttg ttttctacca cttatggcat tcttcttgtt atcaagataa ttcttgttgc
    28441 ggcactgctt gcatcgtttg ttgttcatgt aagaattatc agaaaagacg tagaacaaaa
    28501 aataatggaa aaacaacttt ctgaagtaca aattgtaaga cttcgaaaaa agataatagt
    28561 tgtaggtcag cttacagttg gcctttctat tgtgattttg cttgttgcgg cattattgga
    28621 tgcaggaatc tgagtttccc tcaattcgta cctcatatcc accttcgatc tttcctacac
    28681 catatttgca accagtgatt ttcgatgtga tgtagagatt tgtttcaaga tgttttgaga
    28741 tttcctttac tgtgaaaatt gagagccctt tagttaagct taatggcaat actaacatgt
    28801 cggataaatg caagtccacg ccaaggtcgc agccaatgaa tgtagacgcc gatttattgc
    28861 caaactcagc gcttttctta tcaaacaatt cgtctgatcc aataattgag ctcagatcat
    28921 gactaagaac aagcattgaa gctcctttat cagttgcctt ttcttcgttg atttcaattt
    28981 gtgcagaaaa ttctttcttt tctagtgtgc ttcgtacgtg atctactgct cgtgaaattt
    29041 cgctggcatt cagattagaa taagagcaaa ggatttttgc atttctttcg tttcttttag
    29101 ttaaaaaaag tggcaataat ttcttgctgg gaaaaaccgt tagctcaact aagccgcccc
    29161 cccttggata atatcctcgt ctttgtattt tcattagaaa atcaatccca attctcgagt
    29221 atgcttcagc taaaacgtat tttgtataat ttgcagttgg actccacggc acatccgttc
    29281 ccccgcttat ggaaagctcc agtttctttt ttgcaagtga gacagcgggt attagtgctt
    29341 gtaagataag ggaaatgctt ccagcagtac ccacactttc tttgatgcgc atgtcttgga
    29401 tttcatttgg aaaaaacttg atggacgtag aaccaatctg caatccttct gttttggcgt
    29461 tacaaatttt tgcaagaaga cttaatgtgg ttagatgttg tgctcttagc ccgggaattt
    29521 tcctgttgtg tcttatcttg tcaatttgga ttggtctttt cgttatgcat gagagtgtta
    29581 ttgcgcttcg aagtatttgg ccaccgcctt ctccaaacga accgtcaatt ttcaaacttt
    29641 ccatgatttg tgttttttaa ccaatatgtg tttatgatgg tttttaaatg aattaggaaa
    29701 aaagagaaga catgaatggg ctttttattg gtcggtttca gccgtttcat cttggtcatt
    29761 tagctgccat aaaatttgca ctctcaagtg tagacaatct atcgataggc attggaagct
    29821 caaacagatc caatgaaaaa agaaatccgt ttactgcaga cgagcggaag gaaatgatac
    29881 tgtcctctct tgatgaatcc acattggaaa aaatcaaaat ttattatatt cccgatgtgg
    29941 atgatcatga aaaatggaca tatcatgttg attcgattgt gcctaaatac gacgtggtat
    30001 tttcaaatga tgattttact cataacttgt tcaaaaagcg tggagtcaaa attgtatccg
    30061 tcccattaga acaaagggag gttttgtctg gaactgagat tcgcgcaaaa attgtaaatg
    30121 atcaaaattg gtacgagttt atcccagatg gcactaggaa agttttgtta aaaattaatg
    30181 caaaacaaag actgaaaaat ctgtaattat aaataaagcc ctaaaaacaa accttcaggc
    30241 gatattattt ggaatattta gtcatgttac caggtccaac taacgtacca gagagagtaa
    30301 tgagggcaat gattacacct atgattaatc atagaagtga cgattttgtc gagttatacg
    30361 aggatgcagt tgaaaaaaca aaacaagttt ttcaaagtaa tggtgaggcg gtttgccttt
    30421 cggcatccgg gactggcgca gttgaagctg gtgtaattaa cctaatcaaa aaaggtgata
    30481 aggtcataat tccagtaaac ggcgagttca gtggtaggtt ggctcagatg ttagagtggg
    30541 ctggcgccac ggtgataaaa cttgaaactc aacctggaga aaatgcgagc tttgaggccg
    30601 taaaggaagc atttgacaat aacaaagaca ttaaggcgtt ctactgcgtg tggaacgaaa
    30661 catccacagg aactatgatc aaatatttgg accgagtaaa agatctcacc tcaagaaacg
    30721 attcgtatta cgtagtagac ggtgtctcca ttgtgggcgg tgaggagctc aagatggata
    30781 aatggggaat agacgtcgca atgactggtg cccaaaaggc ctttgctgca cctcctggaa
    30841 tttctccaat tgtagtaaat gcaaaagcaa aaaagtacat gatagaaaat ccaccaaaaa
    30901 caatgtactt taacttgtca agatacttca agtattacga ggaatcaaaa cacactccgt
    30961 ttacgccagc actgccgctt ctttatgcct acagagaggc gctaagcatc attttagaag
    31021 agggtctcga taacagaata cgacgccata gaatctgctc agatgcgctt tactcaggac
    31081 taagcgcgat gggtttgacg ccatttgcca aagaagatgc gcgttcaaca gtagtcgtgg
    31141 cactcaacta tctgcaaggc ttggaggaca aaaccttccg aaatacactt gcacagaaat
    31201 tcagagtgct tgttgcaggc gggtttgggg atctaaaagg caaagtgttc agagttggtt
    31261 gtatgggcga agtgcaaaga taccatgtaa tgagaacaat ttcggcaatc ggctctacac
    31321 ttgagatgat gggatacaaa accaaatcag tggacgcact aaaagttgct gaagaaaaac
    31381 taaaagcact ttagcttctg ttatacttat attaggaaat tcgaagacgg atcgcgttga
    31441 ctttcattaa aggctcacta attttaattg attacactgc aaaagttaag gattcaaacg
    31501 aggtttttga gacaaccatt gaagaagagg caaaaaaaca ctcaatcctt gaagccaatc
    31561 tcaagtacca accaaaacta gtctctgttg gagagtcttg ggtcatcaag ggtcttgatg
    31621 atgcacttac taatgcaaag gccggggaca aactcacagt tgaggttacg ccagacaaag
    31681 gctttggtga acgagatccc tccaaagtta gaatgattcc gttacgtaaa ctaggtgaag
    31741 atgctgagaa ggtctctgtt ggagacacaa tagagataga tcaaaaaacc ggcatagtga
    31801 ggtttattgg ttctggtaga gtccaagttg actttaacca cagatttgcg gggaaaacga
    31861 tcatctatga tgttaacatc ttaaaatcgc tagaatctga tgaagaaaag ataacagcta
    31921 tgttaaagcg acacatacct gtggaggatt ccaagattgt ttcgaaacta aatggtagtt
    31981 ctcttgatgt tgtaattcct gaggagatct ttggcgcaga aggattacga attattaaac
    32041 attttattca gatagatgtt ttcaagttta ttccaactct tgaaaaaatc aactttattg
    32101 aaacctataa taacaaaaag acagaaaaaa ataaagaagt ccccctagaa accaaggcag
    32161 cctaaattac acaagtactt tttgcaagat ctagtgcttt tgtttttgtc atatgaactt
    32221 cttttaggat ggtatatctt tctggtcgaa gcgattgtgc aatgattagt gcctttgcaa
    32281 gcagattccg tttcagacca atctcttttg cagttgttgg tgctccagca tttttcagag
    32341 tttttgagat ttttttccaa tcttggccct gcaattttgc catcagaata gcaccaattc
    32401 cacacttttc tccatggaga ccgactccag gtgcaatctt gtctaatgca tgagaaaaca
    32461 ggtgctctga gccagaacaa ggtctactgc taccagctat gcatgaagca actccagcac
    32521 tgatcaaggc ttcaactact tctctaatgt ctggatgctt tttcttttct gaagcctcga
    32581 ccaaaatttt tgcgctcata gatgccaaat ttgcggaata tctgccaaaa tattcgcctg
    32641 tcttgtctcg tcctatttcc caatctttta ctgcagtaat ttttgcgaca aggtcgccgc
    32701 aaccgcttgc caaaagtctc tttggcgctc tctttatgat gtcaatgtca acaaaaaccc
    32761 cgagcggagc ggttgcaaca agagagtgtg gtctttctcc ccgaatagat acaaaaggac
    32821 ttgcaatccc atcatgtgaa gctgctgttg gcacgctcac aaatggcctt tgtaggttaa
    32881 atgcgatcat ctttgcaacg tcaacggagc gaccgcctcc aattccaata atcaaatcgc
    32941 ttttgtcttt tttaatttcg ttttgaactt ggcgtattga tttttcgtcg ttgtcttttg
    33001 ggccatgcca aattgacctt atttttgatt cagaaagcga tttttgaagt ttctttttaa
    33061 caattttctg tacatgtggt cctgcaataa ctgaaacctt ttttggctta cctagagaac
    33121 gcaagaattc cccaaaatca ccaatgtttt tttctccgac cacaatcagg cgtggaagtt
    33181 ccattgtgtg tgaaggagtg tcttgcatca agttgttgat gaatttactc cttatcaact
    33241 tcccccccaa caaaaagtta tttaaaaggg tgtaaaccat ctcagattat ctaggagtta
    33301 ttttatttgt catctaataa tgtagaggag aagattttac acggaacaac taccgttggt
    33361 attaaggcaa gtaacggtat agtattgtgc gcagacatgc gtgcaagcgc aggctatttt
    33421 attgcaaaca acaacaccat gaaaattcaa cagattgcaa aacacgccgg tatgactatg
    33481 gcgggaggag ttgcagatgc acaaaacata acggatgtct tgcgatacca tgcaaacata
    33541 cacaaggtac aaaaaaatga ggacattccc attagatccc tcgcaagatt gtgctcactg
    33601 ctatttcatc aaaacagagg gtatccgttc attgcagata ttctcattgg tggatatgat
    33661 aaagaaggtc ctgcgctagt aaacatcgac atgtttggct cagtggaaga aaaaaaatat
    33721 gtcacaacag gaagtggctc gccagtggca tatggggtac tagaagatga gtatagtgat
    33781 aacctcaaag ttgaagaagc taaggttatt gcgttacgtg ctgtaaaggc agcaattgtc
    33841 agaaacattg gtacaggtga tggaatcaat gttgcaatta ttgataaaga tggatttcgt
    33901 ctcttaacaa aagagcagaa gaaggccatc ataacgttat agtgatttaa tgcaaagaaa
    33961 acaacaggag aaggaaatac attcaacagc ccaaagcgta atggccacca tactgcaaag
    34021 tattccaaaa gaagcccaag taagcaaaat tgattatgag gggccacgaa ttgcccttta
    34081 cacaaaatcc cccctgtacc tgatggaaca caatgagatt atctctgatc tggtcaatgt
    34141 catcaaaaaa agaattgttg ttagaaccga agaatcaatc agaaaaacag aagaggatgc
    34201 aagacagatc atagcaaagg cattaccaaa agaggctgag cttcaaggaa ccttttttga
    34261 caacgcaact ggtgaaatgt cagttgaggt aaagcgaccg tggcttttac acaacaaccc
    34321 tcaattcaac attgcacagc taacagagag tgcaggttgg cgcctacgga ttagaaaggc
    34381 aacaacaatt ccatctagta ccattcaagc aataaactac aacctaaagg tctcaacatc
    34441 cgatagggca aagcatctca aacagatagg cgaaaacata ttccgaccac gtcttgttca
    34501 aaaatctgaa gtttcattat tgacccttgg cggttttggc caagttggta gatcaagcat
    34561 gatgttagtc acaccagaaa gcaagatctt aatcgattgt ggcatcaacc ctggcgcacg
    34621 taccccgttg gagtcatttc caagacttga ttgggcaaac ctcacgcttg atgaaataga
    34681 tgcgattgtg attagtcatg cacatcttga tcatactgga tttttgccag tcttgtgcaa
    34741 atatggtttc aaaggaccca tttactgcac agagcccact ttaccaatga tgaatctaat
    34801 tcagcttgat gccataaagg tagcagctgc tcagggtaga gcacctatct actcggaaag
    34861 agatgtcaaa caaatcatga ggcaaacagt tacaatccca tatgggactg taactgatat
    34921 tgcacccgac attaaacttg tattttcaaa tgctgggcat attttgggat cagctacttg
    34981 tcacttccac ataggcaatg gcgaccacaa ctttgtgtat actggtgatc tgaaatttgg
    35041 taaaagtatt ttgtttgaaa gtgcttcatg gaattttcca cgtgttgaga ctttgctcat
    35101 tgaaagcaca tacggtgcaa aagaagacat ccagcctact cgacaagaag tagaatccgc
    35161 tttcattaac gctgtaaaca acacgttagt agatggaggc aaggttctca ttccaattcc
    35221 ggctgttggt cgtgcccaag agataatgat ggtgattgat tactatatga aggcgggtca
    35281 gatggtagaa gctccagtgt tcatggaagg aatgatttcg gaggcaagtg ccattcatga
    35341 atcatttcca gaatatctgg cacgagaatt aagacaaaag atacttgaga ctgatgacaa
    35401 tccgtttgat tctgaatatt ttacaaacat tgagcattct gatgcaagag aagaaccgct
    35461 tagggagggt accccctgta tcattttggc tacatctggc atgttagaag gtgggccagt
    35521 attggagtat ttcagaaata ttgcgcctgg aaacaaaaac aaaataattt ttgtttcata
    35581 ccaagtaaat ggcacattgg gacgtagggt tttggatgga gcaaaacaag tccccctttt
    35641 gggaagagat ggtaaggttg aaattgttaa cattaattgt tcgatgataa agcttgatgg
    35701 gtttagtggt cacagcgact acaatcaact actttcattt gtacacaaac tgagaccaaa
    35761 gcttagaagg gtccttgtaa atcatggcga acgaagaaag tgtgaaaacc ttgctatgac
    35821 aattcgcaga atgtttagga ttccatccca ttatcctcag gttcaagagg gaatcagact
    35881 attctagaga gcccaacctc agacatacaa gacatcatgt tttattaaat agccaagccc
    35941 agaaagatac tcggtatcag taatttttcc ttctgtccac caccttacaa catttttgaa
    36001 ccaaggtggt attctcatgt cttcgtattg tattttgata tcctttgtga aaatgtttgt
    36061 ctcaacaagt tctgcagtcc agatagtctt tctgatcctg tcttgatgtt tagcaaaaag
    36121 tactgcgcct gtctgtttgt caataatttc ataatagcta tctgtatgat cttttgcgat
    36181 cagtgcgggt cgtttttgat ccataaatgg atagacgatc tcatcggtta attgggcatc
    36241 aaaggtagaa ccgatttttg ttcctatttg aagtggcatt ttgtgcataa atctatagta
    36301 atcacagcat ttttgatcgg ccagactagc aagaccatcc tcatttattt gaaaagtgtt
    36361 tgttgtcttg ccactttttg agacctcttg tgccaatata ataagcgagt tctcatttac
    36421 attttggaaa gtatgagtta atgtgatcac atcttgtatg ccagtgtttt ttattgagta
    36481 ttttagaaag tcgttttgca cagcgcagat tctattttca cagtaatttg gatcagaaat
    36541 gttggttagt gcagggatca tatccgaggt tatcatgtac cttattccgt atgaaaaaac
    36601 agtgtctttt tcctttcctt gttgccacct tattgcgtca tctttgatcc attctgggat
    36661 ggtttgactt tgtgtaaggc gatctcgtac aggcattggt actgtgggct tgtctaataa
    36721 ggcaacaagt ttttcggtgc gatctgtgtc atttgaatac cataatagat cattgaataa
    36781 aatggtacag ctccgaaaat tcatgtggta ttgatagcgt tgccgaaatt tctcagtccc
    36841 aagtttttct agatctggat aaattttttc gcagctttga atcaatatta ttttctcatc
    36901 agtaccaaca ggtgtttctg cggcagatgg ttttgtattc agtattacta taattgataa
    36961 tgctgtaatt accaaaagaa atttttccaa atcaggaaaa ttgtgttatt ccacgatatt
    37021 aaagagacgt agaaatgata cgttttgttt taatattatt tcagatcata ttctggcttc
    37081 caaattttga cgccaggtgg agttacgatg attctttctt cgccaagcct tgcaatatct
    37141 gcattgtaat tttttgcctg tgcatagaga tcttctacta gcttgcgcaa ctgctcaaca
    37201 tctttttgtg caagcggcgt tactcgtaat atcaatatca tgtttttttt catgtcttct
    37261 ttgattgagt gaatgtcgct tggatcacga attgttattg cttttagata tgttgggctt
    37321 tcttgctttt gcatctaatt atgattgcgg attactccct caaaaagtct ttcgcaacag
    37381 gcatgaagta agtttgtgat ttttgttcct aaattctata tttgatataa tcagactctt
    37441 cggccttatt agttaggatt tccgacttag taccgttacc tttttcaata cttacaattc
    37501 cttgggtttc ttttggtgca tctgaatatc gaagtgtaat tgccgcacaa agatctttat
    37561 gggtttctac catctcaccg cgtaatagtg caattggccc catgtgatct tttacttcaa
    37621 atattaaatc atctggcaaa gctaacgctt tgatcatttc gttttcgtct ttgtttcttc
    37681 caacgattag ttttgatttt tcatccagcc tgaaatgtct tccaattttt agaaggtcga
    37741 tatcgtttgt tgtaggagtt gtgatatgtt tgaagagatc tttggctctt atggcaaatg
    37801 ctggatctgt aagaagacac cccccgcccg catttggcgg attttcaatt ccaaattctt
    37861 ttgccatttg aagttgtatt cttcttgttc ttccacggat catccccatg tcttcccttt
    37921 ttatcaaacc atttttttct ggcattgttt gcgggagtaa tgctgcagat agtggtctta
    37981 ctatgtagcc ttcaagctca gattcttttt caatcgtctt aagagctggg ccgtgttgac
    38041 tcatgggtct ttgacccaat acctcacctg aaatgataaa atcggccccg atctcatcca
    38101 tgtgtttttt tgcagccttg aacatcattg ctctacaatc aatgcaggga ttcatcccag
    38161 agccaaaccc atgttttgga ttttttagca tctcaatgta ttcatctccc aggtaaactg
    38221 ttttgaggtt tactccgagt gtatctgctc gttctctgat ttcaaatcca cagcctcttc
    38281 cacaatcaaa atcgcaaaat ggtgttttta ttgcaactgc agatacctca aatccctgct
    38341 tttgcatcat ttttacagct agttggctat ctaagcctcc cgacaataat gcaacaactt
    38401 tgtgtttttg ttcttccaac aatgcaaaaa gttgaatgcc cgtatacaaa ctttgcaaaa
    38461 catcaaactt ggtatcgaat agttgtgttc ttctagatca gattcgtata aattcgctat
    38521 acgaatacca attttcgact tgaaagaacg tcgaaaaaag ctgatgcaat acgctcagga
    38581 catgaattgt aatgttttag tttcttttga gcctgaaaat cttttttaca tgactggatt
    38641 ctggggcgag gcggtgggaa tacttgaaaa agatggcacc gcaacaataa ttgcgcctga
    38701 gctcgaagta gacagggcaa aaaaggaatc aatagactgc aatgttatca agtctgagcg
    38761 tggggtagga cttctttcta gtattataac aaaaattaat ggtaagaaag tctgtactga
    38821 ttgccaaaat tatgttgcca tggaatcttt gaaaaaatca atcccctcaa taaagcactc
    38881 aactgatccc ttttatcgtg caagagaaat aaaggaccaa acagaagtaa gcactctaag
    38941 aaaagcttcc aaggtaattg accagttgta tgagttgtgc gtaaaaaaaa tcgtggttgg
    39001 gcaacgggaa tcagaacttc aggcaatttt aatgtcatat gctatggaac acaacgcgtt
    39061 tagcactgga tatcgttcaa ctctaaatcc tttgataatt gctgggggcc caaacggtgc
    39121 gttgccacat gctcaggtta ctaaaagaaa atttacaagt ggagacctaa ttgtagtaga
    39181 ccttacacta cgatacaatg gatatgtcag tgatgcgaca aggacgtttg gtcttggtac
    39241 tattagtgat caagtaaaag aggtttatga aatagtaaaa gaatcacaaa ggaagggtct
    39301 caagattgta aaaccaaata tttcttgtaa atcagtcgat gatgcatgta gagaatatat
    39361 taatcaaaaa aactttggac agtactttat tcattctact ggacatggga ttggtcttga
    39421 ggtacatgag ctaccaacaa taagcgcaaa aagtcaaacc aaattgcaaa aaaatatggc
    39481 aataacagtt gagcccggca tctatatccc aaaaaaattt ggagtaagaa tagaagactc
    39541 tgtcattgta aaagacaccc cccaagtttt acataagttc acaaaagatt tgattatcct
    39601 ctagatgttg tccgttttat acacacacca atctaaatta gaacggtttg taggttagac
    39661 agtggagtca ttagttgggt atgtgcttcc aatgaaaatt tggaaacacc caacaatttt
    39721 tatggatttt tcgcagtagc tattagaact ttccaaggaa atacaatctg accgctctct
    39781 tttgtgtaag gaagtgtgtt ttttctgatt cgctctctca ttgaatgtct ttgatggatt
    39841 ggaatcttgt agattttttc tttgagaggt tttgccaaat acctcagata atcattccag
    39901 tagtttgaaa aagtgccagg tcggtactca aaattgtgct gttttatctc aatatttgta
    39961 aaatcagctt ttgctaatgc ttttcttaag ctggtttttg ttccaaatct atcaagtgat
    40021 ggggcaccgg gtggaatgta gtctggaaca aattttatca ctacatcaag tatgctgcta
    40081 aagtaaggaa ctgagttttt ctcaccatga actgagactg cgagagttcc atttttcttt
    40141 aaacagtgtt ttatctgttt taacactgtg tttgtatttg gaaaaaagaa caaagcgtac
    40201 tgacatgtga tggtatcaaa cttttcattg aaaccaatgt gttctgcatc agctatgaca
    40261 aaattcacgt ttggttttgc attattccat ttttttgcaa ttttaattgc agtttgcgag
    40321 ctgtcgattc ctgtaacttg cccactatta ccaacttttt ttagaatttg ttttgtgact
    40381 acgcctgttc cgcatgcaag atcaaggact ttgtaaccgt aacgaatttt tgccatagaa
    40441 actagttttg ctgtactctt gaaagggcca atgttatctt tggcccacct tttatgatat
    40501 ttcggagcta tctcattcca tgtagtaatt attctccttt tgtattctat agaattgaat
    40561 ttaattttct tcggatttcc caaattctct atagtctata tcaaatagct tttaattata
    40621 ttgtgaatga gtactaaaat attggatgca aatattcgag aaggtaggct tcatgatatt
    40681 actcattttg gaataaggct tgcagttggt gtaattttca tagttcatgg gtcggcaaaa
    40741 tttaatccag gttttgttgg gtttttgtcg aatattggct tgccagcaga aatgcagatt
    40801 ccaatagctt tggcagagac ggtgtgtgga atattgctta tcattggtgt tttgagtagg
    40861 atttcctcgt cgttgttatc aattatcatg cttggagcaa tttttcatgt aaagggcgca
    40921 gcaagtttga ctggagaaag aggatttgaa atagatctaa ttttattggc tgcaaacttg
    40981 acagttattg cgattggtcc gggaagggta tcgttgtcac atttagtaaa gaagattcct
    41041 agatttctgc aataaatctt tgaaatctat tctttgtttt tatgattatt attgctggaa
    41101 caacaaaata cattcccatg ttcagcagta tgattccaat cccatatcct aacagttctc
    41161 gttcagagtc aatgtcaacg tagtttagta tcgataaagt tgatagcatt ggtgtaattg
    41221 ttgttttaac tagctccttg aatgttggat tttgcctttc ccagtctgca acagttgggc
    41281 taaatgaata ataaaattgg ttaaaaccaa tcataaatga tgagccagaa ttagtgttga
    41341 gtacaacacc atctctaatt tctcgtaata gctgtacttg tggtgcaagc tcagaaccgt
    41401 atgtagctgt tgcgatcaag cacccaccgt ttttatcatc agtgatgggt gccgcatggt
    41461 ctgagtaggt tggtggcgat gttatcttaa aagatcttat agcttcgtcg aattttggta
    41521 gcgtttcatc aaaattattt tcactgtttg agtatgttat aatatagaat gtttcagaac
    41581 tttttacgat aatttcttta aatttcacgt tgattattcc ctctggggta tcaaacttgc
    41641 ctactgcgtc tgttatgtag gcctcatttc cgttgattga tgttttttct tccatcaaaa
    41701 tagttagggt accatcatct attgcctttt gtagagtttt tttaattgac gctgaatagc
    41761 tttcaagtga agtgccattg gagtttttta ccgaaaatga aattgctggt gtaaaacctc
    41821 ctatcttact tccagctgca acgacgtcag gtgtttgagg gtttcttttt ggtggttcct
    41881 gaacaaacca tcctgcagga ggaacaaatg aaaatccaag tttttcattt gaatattctt
    41941 gtgcagtctg atttgtgagt gcagatactc tttctgggtc accaagatca agaaggtctg
    42001 ggatctctga gcgatttttt atctctgcac ttagtacctc agcttcgccc cacttgagtg
    42061 ggatgccatt tgccgcttct cctgcagtct ccaaatttgc tattttatct agcgtttcat
    42121 agctagtgtc tgttgcaagc cgaccaaatg ccgcgtagtt ttgatccaag aagaatgaat
    42181 ctttgtgaac aatgaaaaac tgtgagctgc cactgtctgg gtctgagccc cttgccatag
    42241 aaacaattcc tcgcttgtgt tttatcgtat taaattcgcc cagtatggta taaccagcat
    42301 cacctgtgcc ccattctgac acatttttgt atgctcctgg ttttgtcttg ggatctcctc
    42361 cttgaatcat gaaatctttg atgaccctgt gaaataccgt cctatcgtaa aacccatgtt
    42421 ctgttaaatt taaaaaattg gcaactgttt ttggcgcgtc ttctgggaag agatctatta
    42481 ccatatctcc cgagtttgtg tgtaaaacca ctactttatc agaaatctct gtttctgcaa
    42541 aaacaggaat tactcctgcg gcaaatacac caataacaac tagaacaaca agactaaatt
    42601 tcaatatggg tcgtttttga gcctcaaata aaagctaatt gtattagttt ttactaattt
    42661 actaaaaagt tagtattagt aaactagaat ttctgtcaaa gcaatcaaaa ttgcacaaga
    42721 aaaactggag aggtgtatca tctttttaga gtttttagaa aataattttc catttaaaaa
    42781 acactctcag taataaggtg ggcggttgga atgacggatc gagtttttta ttgtatgtaa
    42841 gttaattaat ttaataactt ctgtgtaatt acttttatgt tgaaacaaac acaaactctt
    42901 gttctggtca ttgttggaat taatgtacta gctatagtaa ttgcgacaaa cctaattcag
    42961 caacaaacag aagcagaaaa agagagagtg agacacgaac aacaaataca agaaataaag
    43021 aatgcatttt tagaaaatga tattaaaaaa gccactaatc taatagaaag aactgaatta
    43081 aatgaagaag atgtacgaga gttggattgg ataataagtc aggaaattga cagaaggtta
    43141 gatagtctta ctaaatcatt agataattgg tcattacctg attgcacacc tgcacagaga
    43201 gttatgctcg actcgctgga gaagcgttta gagcaatcac agaccataaa agaagccata
    43261 atacatcaaa atcagataaa taatatactt tcacaatgca gatgacttct aaaattggta
    43321 taaagagaga tagtgctcta aatcagtatt tttgaaaaaa gttactaaaa agagagagat
    43381 ggtgcaggtt tccgcttata tgagaaagta ttctacagat cttcccagtg ccatgacttg
    43441 gtttttttgt gataagtgat cttatgatcc gttttagtac catctggtct cgtaaaggtg
    43501 attcttgtat aatcagattt ttctttaaat ttggctttcc aaaccttcca agaaggagga
    43561 gagaaatcta gctttatcat taaatccgcg tcatagatta catcgtgttc tttaacaata
    43621 tccattaatc tatcccaaat atagccaaaa tgttttttcc gtgtcatagt cttactcctt
    43681 caccaatagc atcaaaaatt atttttctat tgtaaggcat actctctctt ttttcatatt
    43741 ctggacatct atgaagatac caattacaca tatcatcaaa tgcataccat tttccatttc
    43801 tgtattgcca gtagattatc tggcggcaat acttgcactt taaattcatg ttctctctct
    43861 ccatttttgg caattctctt ccactaattt ccccatgaat gaatcatcat cctcaatctc
    43921 atctaagatt ggatatccgt gatcgctact accaaataga ctcagtttaa tggcattttc
    43981 aataaagtat tcaggagagc attttaactt cttacaggtg tcatgtaata tgtcagtcaa
    44041 attaatatca agctctattg aaacagtctt taggctcaac ttttcaatac ctcacttgaa
    44101 attatgaatt gcttatcgtc taatgcgtag catttgtcac agtaatcaat ggtgtaattg
    44161 cctgcacttc caccatcaaa tgttatacgg tgagttttct ttgatgcaca tccacactct
    44221 ctcaatgtcc agccctccca agtaattcaa ttattatggc ttcataggac tggccaaaac
    44281 gccctaattt tgcaagtctt tcctttgtgg atggatttag atttattgtg gtaaattttg
    44341 acatgtatta ccttagatta gctacaataa gagaagtaat tactcaatgg cgaacacgtc
    44401 gccattggtc tcattaggat cctattttgt ttccttcact acgattgtct tttctaatcg
    44461 ttcttggcgc tttcttacct tttctaaatc ctctttcatc tcaatgatta actttgtgag
    44521 atgttcaatt tctttgttct tagattcaag ctgttccttg aacaacccgt ttgttgcatc
    44581 tgagtttcca aaaattaaca gagctggctc taatgttttg tacatttttg ctctttcttc
    44641 tggcgctatt ctgtaatatt gccccaaata gctagtatga ccatctaacg cattagcata
    44701 ttcagtacta tgaattcttg atgcgtttgt gtggaaatac gcacgaaaag catgaatgtt
    44761 tacaacaaat ctgctactgc cttcatattt ttctaaaaga ccacaccttc gtctcaattt
    44821 actaaaaaca gcttcttcaa cacgaaccgc ggctacattg tttttctgat tagcaaacac
    44881 gagttcagag tcgct
//
LOCUS       DASWKH010000081        24287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2805493, whole genome shotgun sequence.
ACCESSION   DASWKH010000081 DASWKH010000000
VERSION     DASWKH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 24287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24287
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2805493"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="pgk"
                     /locus_tag="VGA92_06180"
     CDS             complement(<1..755)
                     /gene="pgk"
                     /locus_tag="VGA92_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9846037.1"
                     /translation="MAKKTKGVKILTLADFDLKGKTVLLRVDMNCPIDPQTGQISGAK
                     RIEEVTETINALNDAKVVVVSHQGRVGNTDYIGMSEHAKILEKLLGKKIKYVEDVIGA
                     LAQEEIKKMKAGEIILLDNLRFCAEENYEFTPEDAANTIMVKRLGGLFDLCVLDSFPS
                     AHRAHPSLVGFSHIIPACAGKIVEREVRKLDEIITIAKAPHVIVLGGSKITDRLEAIK
                     MLIKRGRADYILLTGLIANVFMRAQARIKYPLG"
     gene            923..1516
                     /locus_tag="VGA92_06185"
     CDS             923..1516
                     /locus_tag="VGA92_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9846038.1"
                     /translation="MAIMVNAKATSDFDKSAITYFQSVAGNHAVDVAMWVITEIGDVW
                     WLVLFSIAIFIIRKTRRMGLILILALVAGTIAAGYIKGYVVDNPGPKLEFLGTELPFE
                     IGQDTFVLGTDGSFPSGHATRAAVVALIIGYALSQRFPRGCYLIWIFPVLESISRVYV
                     LQHYPMDVFGGTILGVLLAGVIGKKLKLSETFKKSQA"
     gene            complement(1505..1816)
                     /locus_tag="VGA92_06190"
     CDS             complement(1505..1816)
                     /locus_tag="VGA92_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846039.1"
                     /translation="MDFHPSQIPIVKTFVIKDEKAASDSAAEMVSLGFENQKGGYKVL
                     MPNEDKLAKRIGFAITTEINYKLRQTKQDRNLRYWTYHHDNENYAIVLISAKVFDDLG
                     L"
     gene            1960..2853
                     /locus_tag="VGA92_06195"
     CDS             1960..2853
                     /locus_tag="VGA92_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEX9846040.1"
                     /translation="MTSLEKVVETLKRQKLEASKLRRQSERKLKEAKSLARRSSSGLH
                     SVQRKLEDTREKLGEINAEFTQFLARKESLERLIKAANDRLSQEMEAKAQAQIDLENA
                     DSQEEKQAASERLAQIIEKIQELKSEIKQRESSSQKLLPVIEVQKKTKSKTSQQIHKQ
                     VQTKPTLVTLIKKGTTESERLKRRVDIAAKKEMSISKNLHEAAQKLGELLAKKRREAA
                     KRAALKRAAKKKALALKRKQAALKRKLAMKKKHAKKSKKTKKPKKAKSKTKAKASKKS
                     KTRAKSKKSSKKKSSKKKSRR"
     gene            2897..3499
                     /locus_tag="VGA92_06200"
     CDS             2897..3499
                     /locus_tag="VGA92_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846041.1"
                     /translation="MIKKRGIYVAIGGVVMIVVAFAITTSIMTDQGFDRDGFSLPDVL
                     EGMFDQVSDETQLAPGETAYFSFDASADTKMLFWGVQILDYQSGDSVLVSISNIYGDD
                     FGQFTVDQPAFFETMETENDNSYNFNVENRAGRTITVVMMFTKNPQDSNMFSDPNSPL
                     SKTLVPLAISGALLIIGVIAVIAGIVIIIIDYKKRRSEFV"
     gene            complement(3496..3792)
                     /locus_tag="VGA92_06205"
     CDS             complement(3496..3792)
                     /locus_tag="VGA92_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9846042.1"
                     /translation="MVTTDQAKNMRNKIDIEGTVERMEEPRTVNLKVGGTTEVCNAFL
                     VDEAGEIKIALWGEDIKKVKNGSKIIIKNGYTNTFKGEVSLGTGKYGEIIVKEY"
     gene            3933..4030
                     /locus_tag="VGA92_06210"
     tRNA            join(3933..3972,3986..4030)
                     /locus_tag="VGA92_06210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3969..3971,aa:Leu,seq:caa)
     gene            4138..4596
                     /locus_tag="VGA92_06215"
     CDS             4138..4596
                     /locus_tag="VGA92_06215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEX9846043.1"
                     /translation="MFIPKIEKRTSKIEGLGLFANKKIFKGEIIWTFTPGTVKVIAVD
                     EFEKLPKDEQQVWVDHCYQMGDRFYMDVDDARLMNHSCEPNIIDNPNDGAATLVAARD
                     IEKDEEVTWSYLPYMNPYQAFSCYCGSKHCVGTVKKGAVVNSAKIKIEQR"
     gene            4676..6787
                     /locus_tag="VGA92_06220"
     CDS             4676..6787
                     /locus_tag="VGA92_06220"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxypropionate--CoA ligase"
                     /protein_id="HEX9846044.1"
                     /translation="MVSTKKIFDETIKTDHKVITEEASKSILKSYGVKVPPYALVTTP
                     KQAVKAAKKIGYPLVMKIVSPQILHKTDVGGVKVGIDNDKDVKKTFNDMYKRLSKKRG
                     VEVKGVLLEKMVPKGVELIVGLQVDPQFGPAIMVGLGGVMTEVFKDVAFRMLPITTSD
                     AKSMLDELKGAKILKGFRGSKPIDMDMLAKALVQIGKLGVENADYVNSIDFNPVVVYP
                     KSYYVVDAKIILGNQIHKNAISKAQPDDSFMEKFFTPQSVALIGASATPGKIGNSVLD
                     SLAKHDYKGQVYPINPKAEEILGLKCYPSLEAINGPIDLVVVCVDLSVTPPILESCAK
                     KGIHNVVIVSGGGKELGGERADYESQIKELALKHKIRIIGPNCIGMFNAANRLDCAFQ
                     GQARMVRARLGNVALLSQSGTMGISFLETADSFGLSKMVSYGNRSDVDEADMIWYLAS
                     DPQTKVIALYVEGFGDGRKFVETAKRVMKEKKKPVVIWKSGRTEAGAKQAASHTGSLG
                     GSNAIIMGAFKQAGIISVDSYQELAAVVKALAWQPAAKGNRAALCSNGAGPMIGGIDH
                     FERLGLELAQVTPQTLKELKEHFPPTYVIGRGNPVDVTGGANADDYRYTIQKFYDDPN
                     VDIVMPWFVFQDDPLEETIIQYLAEFSRQQKKPLLVGGNGGPYTQKVSALIEKEGVPV
                     YDDIRSWVAAASALVQWGKHL"
     gene            6825..7586
                     /locus_tag="VGA92_06225"
     CDS             6825..7586
                     /locus_tag="VGA92_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9846045.1"
                     /translation="MALVTISKSNGIATVKINRPDKLNAMNVDTAKEIIQIFTELDKD
                     GEVKVIILTGEGEKAFSAGADIEYMSKISADESVEYAKLGQLLTATVENVSQPTIAAV
                     NGFALGGGCELAMSCDIRIAADTARLGQPEVTIGIPPGWGGTQRLMRIVGIAKAKELV
                     FTGKMIKADEAKEIGLVNQVVPLASLMDETLKMAQQIAANSVSGVRMSKIAINKGRNA
                     DLDTGLGVELLAWRNCFTDPDRAERMTAFVNKSKK"
     gene            complement(7686..7895)
                     /locus_tag="VGA92_06230"
     CDS             complement(7686..7895)
                     /locus_tag="VGA92_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846046.1"
                     /translation="MLELGKMEEGRDHDMNLYPMSCKAETGLDSDRLPQELRECTPAL
                     QDGPSKNGLEIKSSVDEAGRVAVAT"
     gene            8059..8409
                     /gene="erpA"
                     /locus_tag="VGA92_06235"
     CDS             8059..8409
                     /gene="erpA"
                     /locus_tag="VGA92_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9846047.1"
                     /translation="MATEQSQKLVTITPKAAEKIVEFMKEEAEKPEYLRVYVQGGGCS
                     GLSYGMGFEAKPEEDDTVIEEAGVKLLVDSYSQEHLQGANIDYIESLMGSGFKINNPN
                     VTKSCSCGHSFSTE"
     gene            8540..9688
                     /gene="dnaG"
                     /locus_tag="VGA92_06240"
     CDS             8540..9688
                     /gene="dnaG"
                     /locus_tag="VGA92_06240"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HEX9846048.1"
                     /translation="MPPTGIVKYHVKLSFEVDGLVERADIIGAIFGQTEGLLGPEMNL
                     NELQRMSKVGRIEVTTNTTTNTTKGDALIPMSTDVDTASLIAAAIESIDKVGPFDCKF
                     LLGAIEDVRATKKDDIIKRAKEIKQQWATKSISESDTMLKDIHEGTSSSGKLTTYGKG
                     KLPCGVGVFESPWIILVEGRADVINLIRAGIDNALAIDGARIDESIKDLCDTKEKVIA
                     FLDGDRAGGFILKELKSVVDIDVIHRAPEGVEVEELTPQQITDILKDTIDEIKNPKPK
                     PTLSDPEDKSIAELAEKIFSQINETLEAIGLDANRAEVFKVPISELVSKLSVQSGIKY
                     LILDGIVTQRLVDGAKQAGIECVIGHRIANLTNQDGITLKTFTDLGVA"
     gene            9698..10375
                     /locus_tag="VGA92_06245"
     CDS             9698..10375
                     /locus_tag="VGA92_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX9846049.1"
                     /translation="MSELKIQHLVTLTELLTKGAKNNFVSITTSSLGKNIHKSQQAAS
                     KHLLELEEAGFIERIMSGRKISVKLTKKGYDQISDVLNLIKKGLESIPSVIELKGVLI
                     AGMGEGAYYMSLKGYTKQFKTKIGYVPFPGTLNVKLDKKEHIEAVRQLNGIEGVKIEG
                     FSDGKRTYGWVKCHRCTLNDRVDCQLILLERTHHDNSVIELISETDIRKKLGLKNGSE
                     LTIKIPI"
     gene            10443..11138
                     /locus_tag="VGA92_06250"
     CDS             10443..11138
                     /locus_tag="VGA92_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX9846050.1"
                     /translation="MNKKMLIAAIIVGIGVPITVYAASPLFINTTIDEPLPTNIMKTD
                     EKMMEEKMEKEMTEEKAMLKDEMMESEAMLEEEMTEEMIKEKMMDKESVMETQTMEEE
                     MMEKTMEETMMEKQTTQSSDSEDFSAKSGVFVGVGDGIHDAQGNALVIPLENGQKVLR
                     LEDFRSTNGPDLYVYLATDSGASDYVSLGRLKANIGNQNYDIPAGTDLSKYDNVLIWC
                     KQFSVLFGSAELA"
     gene            complement(11156..11773)
                     /locus_tag="VGA92_06255"
     CDS             complement(11156..11773)
                     /locus_tag="VGA92_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX9846051.1"
                     /translation="MDIIDKKILGIFYVCNITKEQPMDICIKRMQLSEKYLSERMDNL
                     AKNDLITKDKKSLTEFGRTALHVVLAGGVFDIIHPGHIHTLNAAKVLGDVLVVVVATA
                     STAVKMKKRTPLHTEEHRQELVGSLSMVDLCLVGREGDIFKTVEVVKPDIIALGYDQV
                     HQEKFITDGCKNLGLEVRVARLQSPIPEFSSSKIEKEYGEALHGI"
     gene            complement(11810..12850)
                     /gene="dph5"
                     /locus_tag="VGA92_06260"
     CDS             complement(11810..12850)
                     /gene="dph5"
                     /locus_tag="VGA92_06260"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HEX9846052.1"
                     /translation="MLWFIGLGITGPDSLSENTKKIISDSDVVYFEQFTSPMPDSDVQ
                     KIKQLTKGEFKFAPRWLVEDGKEILENAKSKNISLLSFGDPYIATTHIELRTRAIQEK
                     IKTGTVHASSALTSLVGECGLHYYKVGKTVTIMSGIPSTTAYYTVFENLKLGNHTVIL
                     LEYNQNKNFFLDPKEAFQSLLATEKEQTRKVISDSMYAIVASRIGQESQKIISGKISS
                     LANVNFGAPPHSIVIPGALHFTESDALKVLAECLDEPADNSEKIEKISAQMMKKYVPM
                     VRRALEQIIPHYKDQKEFQGVLENAELYIRDAERFFEQGKDELAILSIGYADGLVDAL
                     RIAKGIEPELNS"
     gene            complement(12851..13981)
                     /locus_tag="VGA92_06265"
     CDS             complement(12851..13981)
                     /locus_tag="VGA92_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9846053.1"
                     /translation="MDSSFTITPRFSIKLLEKALRLYTPSLAEKPLAEFLADKCDDLG
                     FDNIRIDEVGNLIATIGSGSPTVLLCGHMDTVPGKVKVRKEGDFLYGRGASDAKAPLM
                     AMLLAASTMQKNNGTIIFVGAVDEEGNATGIKNLAKQKLDIDYAVFGEPSGIRNVTIA
                     YKGRIAINLKVNVGDSAHASAPWLAKNAIEEALKFTAELRKSLEENQEGKSKGMILTA
                     TPTEIRGGDSHNVTPKECDTIMDIRIPVTMNCKSVGEKIATLVNEISKKQGVEAIYSV
                     LDETEPFEAPLDSPLIRAFTLGVMDVEHTRPSLIRKTGTGDMNIIGTLWNIPVVTYGP
                     GDPHASHTMDERVSMNEYLRGIEVIKNTLHHLKRLHDSKKGK"
     gene            complement(13985..14830)
                     /gene="lysX"
                     /locus_tag="VGA92_06270"
     CDS             complement(13985..14830)
                     /gene="lysX"
                     /locus_tag="VGA92_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX9846054.1"
                     /translation="MTHSITVLYDTIRLEEKALVEAAAKSGIKIEMVDCKNLILDIDK
                     KTEYHTVLQRCVSYYRNMHSTAALEGMGANVINSLHTGIYAGNKLFTHMLLQKMKIPT
                     PYVTVAFSKDAALEALDKLGYPRVIKPTIGSWGRMISKLNNRESAEGIIEEREKMYPI
                     YQVHFLEEFVQRPPRDIRAIVIGDTVAGAIYRNSTEENWKTNTHLGGTAEVCKVTKEL
                     EDLCIKAKDAVKGQIVGVDLMESKDKGLVVHEINNTTEYRNVARVTGIDIAAKILEYA
                     VNSGK"
     gene            complement(14827..14994)
                     /gene="lysW/argW"
                     /locus_tag="VGA92_06275"
     CDS             complement(14827..14994)
                     /gene="lysW/argW"
                     /locus_tag="VGA92_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HEX9846055.1"
                     /translation="MTKCTECDSDISIPKDALEGELVTCPDCGASFELIKSSDGFGLK
                     PAQSVGEDWGQ"
     gene            complement(14997..16178)
                     /locus_tag="VGA92_06280"
     CDS             complement(14997..16178)
                     /locus_tag="VGA92_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9846056.1"
                     /translation="MSDPNYYAHLYNEYEKKPKKIRVLDSTLREGEQHPGVTFTNKQR
                     IQIAWMLDYFGVDQIEISPVVSEDHLEATKTIIKQGLKADIVAHVRALKSDVDIALKC
                     DPKWTATYLGISDIHMKDKLRITREEALRRAVETVEYAKSHGLKMRFTVEDGSRADPE
                     FLLKVCKAIEEAGVDRISLPDTVGVMRPKGMYNFVKSIREKVKVPLDVHCHNDIGFAL
                     ANAFAGVDAGADQIHTTIDGIGERTGIPALAEVAVALTYLYKSPNDFRLDMISDLSRL
                     IESYTGVTPYDSKPLVGSSAYKHKAGTHLAAILRNPAAYEPIPPRVVGNRRRIVFGEL
                     AGKTGSAYLMSILGLEKDDEQAKAVALGLKNLRMGDLLEIPLDSKLERKIINDSKGTK
                     E"
     gene            complement(16296..16715)
                     /gene="lysM"
                     /locus_tag="VGA92_06285"
     CDS             complement(16296..16715)
                     /gene="lysM"
                     /locus_tag="VGA92_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator LysM"
                     /protein_id="HEX9846057.1"
                     /translation="MYKDKIDDEILKILKEDSRESFVDIGKKLKLSESAVRRRVKNLS
                     DAKIIKKFTIEIGEQNSTKAIVLISVESSTDTSKVSARLTKLEGVKTVYEITGQYDIA
                     VIISSPNITEINAAIDSLRKVPGVSDTNTVIILREIT"
     gene            complement(16696..17886)
                     /locus_tag="VGA92_06290"
     CDS             complement(16696..17886)
                     /locus_tag="VGA92_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9846058.1"
                     /translation="MTEDDFLGNLYQRFPVTIERGLGTRVWDINNKEYIDCMGGYGVA
                     LVGHRNPRVVEAIKKQLDKIITVHTSLYNKTREEFLENLIKAAPKGLSQVHLGNSGAE
                     AVEAAIKFARKFTGKSGMIAMNGSYHGKSMGALSLTFNPKYRKSFAPLVEKVTFSPFG
                     DIESLRSKIDSDTAFVIMEPIQGESGINVAPDGFLQDVRKLCDERGILLIFDEIQAGL
                     GRTGKMWACEHWNTAPDIMCLAKGLAGGIPISATLVKPEVLAAMSKGEHSSTFGGNPI
                     SCAAGIATLQALTQDGLIQNAVTIGKKFKEGLENLKEKHSIIRDVRGKGLMIGVEMKY
                     EVKDILFDGISEGVLLLYSGRNILRFLPPLVLSEADITKVLEVLDKLFTREEERRKNV
                     QGQN"
     gene            complement(17883..18683)
                     /locus_tag="VGA92_06295"
     CDS             complement(17883..18683)
                     /locus_tag="VGA92_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate/[LysW]-glutamate kinase"
                     /protein_id="HEX9846059.1"
                     /translation="MITIKIGGSVVENLHPSTIPDIKKVAQSEGVILVHGGGKEVTKT
                     CELLGKEPKFVVSPSGIKSRYTDKETAEIFTMVMSGKINKNIVQMLQKNGINAVGLSG
                     VDGQIFLAERKDKLVIINEKGRKQVIDGGYTGKVTSVNASLLKLLLDQGYTPVISPIA
                     ISKEFDFLNIDGDRAAANVAGATKSDKILFLTNVDGLLMNDKLVEKMTNAEAKEIRPK
                     VGFGMEKKILAASEALDMGVSEALIANGQRENPISSAISHTKCTVITR"
     gene            complement(18687..19742)
                     /gene="argC"
                     /locus_tag="VGA92_06300"
     CDS             complement(18687..19742)
                     /gene="argC"
                     /locus_tag="VGA92_06300"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9846060.1"
                     /translation="MLRVGVVGASGFVGGEMLRLLVSHPKVEIAMVTSRQYVGEYLHR
                     IQPSLKGFTEMTFSELDYDKMSDKCDLVFTAVPHGTATEIVKALYDRGLKVVDLSADY
                     RLHNQAGYDKWYGWEHPHPDYLAKSVFGIPELHREKIKDAKLVSCPGCMAVTSILALA
                     PLIKNNLIDTEHIIVDSKIGSSGAGAGSVAGTHHAMRSGVIRPYKPAKHRHTGEIEQE
                     LSEIAGKPIHVSMSPHAVDIVRGILCTNHTFMLKDMTELELWKVYREMYKDEKFIRLI
                     RDKKGLYKFPDPKFVVGSNFCDIGFDIDEDNHRLVALSASDNLMKGAAGSAIQNMNVM
                     SGFDEMDGLRYSPLTPV"
     gene            complement(19770..20627)
                     /gene="lysX"
                     /locus_tag="VGA92_06305"
     CDS             complement(19770..20627)
                     /gene="lysX"
                     /locus_tag="VGA92_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX9846061.1"
                     /translation="MSKICIVFDRVRPEEKMLEDEASKLGHNTTKVDAKITQLNTDSV
                     RSDFDFGDVVLERCVSYFRGLHFTAALEFLGVPVINSFDVSDRCGNKMITSLLLRKAG
                     VPTPRTYFAFSSEEATKLIEKVGYPLVIKPVIGSWGRGILPLRDKDTTEAIIEMRDIE
                     DGSHDRIYYLQEMIDRPPRDIRAITVGDQVVAAMYRKSEGDFKTNIALGAEPELCQIT
                     KELEDLCIKASKAVGGGILGIDLMEDKKRGLVVHEVNNTVEFKGLAKVAKKNIPQEMV
                     KFAITHITK"
     gene            complement(20628..20795)
                     /gene="lysW/argW"
                     /locus_tag="VGA92_06310"
     CDS             complement(20628..20795)
                     /gene="lysW/argW"
                     /locus_tag="VGA92_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HEX9846062.1"
                     /translation="MKCSECDAGINVPDDASVGEIVSCPECGADFEIAQKEGATIQLK
                     PAETVGEDWGE"
     gene            complement(20810..21997)
                     /locus_tag="VGA92_06315"
     CDS             complement(20810..21997)
                     /locus_tag="VGA92_06315"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9846063.1"
                     /translation="MKQKAVLAFSGGLDTSVVVRYLQEKHDMDVITVTVDVGEGSDQR
                     KIAAKAKKLGVLRHYNIDAKKEFVTDFIHPAIKANAMYQKKYCLATSLARPLIAQKVL
                     EIAKKEKVNALAHGCTGKGNDQVRFDVTFASGSNLPIIAPIRDLNLNRVTELKFAKKH
                     KIPVENVSKKFSIDQNLWGRAIEGGNLEDPYSEPPADAFIWVKTKNLPDSPKYLEIEF
                     ANGIPVAVDKKRMDPIKLIEYTNKVAGSCGVGIVDHIEDRVVGIKSREVYETPAALCI
                     IEAHSDLEKMVHTKHQTKFKDLADAEWAWLAYSGLWQDPLRADLDKFIDSSQKVVSGT
                     VKIKLYKGGLRVVGRKSKYSLYSHHMATYGKGSAFDQKKAAGFVELWGLQTTEANKLL
                     RKR"
     gene            22186..22431
                     /locus_tag="VGA92_06320"
     CDS             22186..22431
                     /locus_tag="VGA92_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9846064.1"
                     /translation="METTMKFTNNVGKRKFTMLVQEEPEGGFTGKCLELRGAISYGKT
                     MKELKANMKDAIALILDTLEEEAEKGNYRKEVIEISS"
     gene            22445..22681
                     /locus_tag="VGA92_06325"
     CDS             22445..22681
                     /locus_tag="VGA92_06325"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9846065.1"
                     /translation="MSLKNHDWRKIVKTLSHFGYVPVRQSGGHIILRNSKGMIVSVPR
                     YDPLPEGTKSNSVRSRNQKRGFHKKVVVNEKCDF"
     gene            22792..23802
                     /locus_tag="VGA92_06330"
     CDS             22792..23802
                     /locus_tag="VGA92_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9846066.1"
                     /translation="MKIRLLVGIAAAAIVLSAIVLAGTAAKTSDQNKIRVAFFPNISH
                     AVPIVGTENGEFAKELGDIPIQTVIVDSGPQAIEALFANSVDIAYVGPSPFINGYVKS
                     GQGIKIISGAANNGASLIIQKDSEISGISDLAGKKIAAPSIGNTQDVSLRHYLSEYDL
                     RPAERGGSVIVYNTANSETFTLFAKGDIDAAWVPEPTATILVEQLGGKRLFNEEELWP
                     NKQFPSVLLIGRTDFIEKHPGWIKSWLAAHQNTINWINQNPEKTEAVFIDFYKKHTGK
                     VLPENIVHGSFSHIVFTSDLDEVAMMTFAARAYELGYLGRSQYNIDDIFYNSTEDISW
                     QN"
     gene            23790..>24287
                     /locus_tag="VGA92_06335"
     CDS             23790..>24287
                     /locus_tag="VGA92_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9846067.1"
                     /translation="MAKLEAKNIIKHFDHDGQKLIALGGVNLTVEDGDFVCLVGPSGC
                     GKSTFLRIAAGLQKPDEGQILLDNRPINSTGPDRILVFQEGALFPWLKVRDNVEFGLK
                     IAGIPESERHQISDRYLEMMQLTKFANSYTYQLSTGMKQRVSIARALVMDPAVLLMDE
                     PFASLD"
BASE COUNT         6549 a         5450 c         5399 g         6889 t
ORIGIN      
        1 atgccaagcg ggtacttgat tctagcctgg gcgcgcatga acacgtttgc aataaggcca
       61 gtcagaagaa tatagtctgc gcgaccgcgc tttattagca tcttgattgc ctcaagcctg
      121 tctgttatct tggagcctcc caatactatg acatgcggtg ccttggcaat ggtgattatc
      181 tcatctagct ttcttacttc gcgctctaca atttttccag cacatgccgg tatgatgtga
      241 gagaagccga ctagagacgg gtgggccctg tgcgcactag gaaacgaatc taaaacacac
      301 aaatcaaaaa gacctccgag tctttttacc atgattgtgt ttgcggcatc ctctggagta
      361 aattcataat tttcttctgc acaaaatctc agattatcaa gcaagatgat ctcgcctgct
      421 ttcatttttt ttatttcttc ttgtgcaagt gcgccaatca catcttcgac atattttatt
      481 ttttttccaa ggagtttttc taaaatcttt gcgtgctcgg acatgccaat gtaatccgtg
      541 tttccaaccc ttccttggtg cgagaccacg acgacttttg catcgtttag tgcattgatg
      601 gtctcagtta cctcctcgat tctttttgcg ccggagatct ggccggtttg aggatcaatt
      661 gggcaattca tgtcgactcg aagcaacact gtctttcctt tcaaatcgaa atcagcaagc
      721 gttagaatct tcacaccctt tgtctttttt gccatcttaa aatctttgag ccgatctagc
      781 gtgcacaaaa ttctacaaaa cacgttgctg gcgtcttgca gaaaaattaa aaacaagact
      841 gccgagtcct gtttgattgc aaaactggtt atttgatgta cggtctagac cgtttcttat
      901 tttgagtgtc gcattcgtca taatggctat tatggtaaac gcaaaggcca catcggattt
      961 tgacaaatct gctatcacct actttcagtc cgtcgcagga aaccacgcag tcgatgtggc
     1021 aatgtgggta attaccgaaa taggcgacgt gtggtggctg gtgctcttta gcattgcgat
     1081 atttatcata agaaaaacca ggaggatggg actgattctg attttggcgc tggtggcagg
     1141 aacgattgcc gcaggctaca tcaaaggata cgtggtggat aatccagggc caaaactcga
     1201 gtttttggga actgagcttc catttgagat aggccaggac acattcgttt tgggcacgga
     1261 cggctcattt ccatccggcc atgcgacacg ggccgcagtt gtggcactga tcataggata
     1321 tgcgttatca caaaggtttc caagaggatg ttatctgatt tggatatttc cagtactaga
     1381 atcgataagc agggtatacg tacttcaaca ctatccgatg gatgtttttg gcggaacaat
     1441 tcttggggtg ctgctggccg gagtaatagg caaaaagcta aagctttctg aaaccttcaa
     1501 aaaatcacag gcctagatca tcaaaaactt tggcactaat tagaacaatg gcgtaatttt
     1561 cgttgtcgtg gtggtatgtc cagtatcgca ggttacgatc ttgttttgtt tgccgtagtt
     1621 tgtaatttat ttcagtagtt attgcaaatc cgattctttt tgccagcttg tcctcatttg
     1681 gcattaaaac tttgtatccg cccttttgat tttcaaatcc caagcttacc atctcggctg
     1741 cagaatcaga cgctgccttt tcgtctttta ttacaaatgt ttttacaatt gggatctgtg
     1801 atggatgaaa atccacaaaa caaatcagaa caagatcaat ttaactagtt ttttcgtaca
     1861 aatcgcaaga tctggcagta tggtgttaaa cttgttaact cacaagtcaa atactggtta
     1921 tagttataac ttatcgataa tcaaatagac caaaacttgt tgacctcctt agaaaaagta
     1981 gtagaaacgc taaagcgaca aaagctagag gcatcaaaac taaggcgaca gagcgaaaga
     2041 aaactaaaag aagccaagtc tttggcccgg cgttcatctt ctggactcca ttccgttcag
     2101 agaaaactgg aggatacgcg agaaaagcta ggcgaaatta atgccgaatt tacccagttt
     2161 cttgctcgaa aggaaagcct ggagcggcta atcaaggctg caaacgaccg cctctcgcag
     2221 gaaatggagg caaaagcaca ggcgcaaatc gacttggaga atgcagattc tcaagaagaa
     2281 aaacaggcag catctgagag gcttgcacag ataatagaaa aaatccagga gctaaaatca
     2341 gaaataaagc agcgggaatc ctcatcacaa aaactcctcc ctgtaataga agtccagaaa
     2401 aaaacaaaat caaaaacaag ccagcaaatc cacaagcagg tgcaaacaaa acctacccta
     2461 gtcaccctca taaagaaggg cacaacagag tctgagcgac taaagaggcg agtagatatt
     2521 gcagcaaaaa aggaaatgtc aattagcaaa aacttgcacg aggcagccca aaaactcgga
     2581 gagctcctag ctaaaaaaag acgcgaggcg gcaaaaaggg cagcactcaa gcgagctgca
     2641 aagaaaaagg cacttgccct aaaaagaaag caggcggcac tgaaaagaaa acttgcaatg
     2701 aaaaaaaagc atgccaaaaa atccaagaaa acaaaaaagc caaaaaaggc aaaatcaaaa
     2761 acaaaggcaa aagcttcaaa aaaatcaaaa acaagagcca aatcaaaaaa atcctccaaa
     2821 aagaaatcct caaagaaaaa atcaaggcgt taatttcaat acatacatta tacgctggta
     2881 agctgattta cacttgatga taaaaaaacg cggaatttac gtcgcaattg gcggtgtggt
     2941 aatgattgtt gtcgcttttg caataactac gtccataatg acagaccagg gatttgatcg
     3001 ggacggattt tcattgcctg atgtcctaga ggggatgttt gaccaagtat cagatgagac
     3061 gcagcttgcg cctggggaaa ctgcatactt ttcatttgat gcctcagcag acacaaaaat
     3121 gctgttttgg ggagtgcaaa ttctggacta tcaaagtggc gattctgttt tagtttcaat
     3181 ttcaaacatc tacggcgatg actttgggca gtttactgtg gaccagcctg cgttttttga
     3241 aacaatggag acagaaaatg acaactctta caactttaat gtggaaaatc gggcaggcag
     3301 gacaatcaca gttgtaatga tgtttacaaa aaatccccaa gactccaata tgttttctga
     3361 tccaaactcg cctctatcaa aaacactcgt accgcttgca atctccggcg cgcttttgat
     3421 cattggtgtg atcgcagtaa ttgccggtat agtaattata ataattgatt ataaaaaaag
     3481 acgttcggaa tttgtctagt attcctttac gatgatctcg ccgtactttc ctgttcctaa
     3541 agaaacttcg cctttgaagg tgtttgtata gccgtttttg attattattt ttgatccgtt
     3601 ctttactttt ttgatgtcct ctccccacaa agcaatcttg atctcgcctg cctcatctac
     3661 taggaatgca ttgcatacct cggttgttcc gccgactttg agatttactg tgcgtggttc
     3721 ttccatccgc tcgactgtac cttctatgtc gattttgttt ctcatgtttt ttgcttggtc
     3781 tgttgttacc acaaattggg tttattctga actaataata agttttttta gaatattagg
     3841 aattcaaaat ttcccagttt ttttgaggta atttgagaaa ccgacctctg aatgctatat
     3901 cagttatata ctaatatttg aaaaatgctt ttgcaggggt atcgaagccc ggtcaaccga
     3961 gagggactca agatcaaaat aagacatccc ttccctcagg ggttcgtggg ttcaaatccc
     4021 accccctgca cttttcctcc atgccacatc acagattgcg catatgcaga ttcacaaaac
     4081 caggaatggg cacagggatg ctgtcgagtt ataagtgcga aattgtatgg tgtcataatg
     4141 tttatcccca aaatagaaaa acgcacctca aaaatcgagg gcctgggatt gtttgcaaat
     4201 aaaaaaatct tcaaaggcga gataatctgg acgtttacgc caggcacagt caaagtaatt
     4261 gcagtcgatg aatttgaaaa gctgccaaaa gacgagcagc aggtgtgggt agaccattgc
     4321 taccaaatgg gcgacagatt ttacatggat gttgatgatg caaggctgat gaatcacagc
     4381 tgcgagccaa acataataga taatcccaat gatggcgcag cgacacttgt tgctgcaaga
     4441 gacatagaaa aagacgaaga ggtcacctgg agctatctgc cttacatgaa tccgtatcag
     4501 gccttttcat gttattgtgg tagtaaacat tgcgtgggga cagtaaagaa gggagcagtt
     4561 gtaaatagtg ccaaaatcaa aattgagcag cgctgaataa atgatgatct tggcaaaatc
     4621 aactggactc tgaccaaaat ttaatatatc gagctagaat gggatcgatc acgacatggt
     4681 ctccactaaa aaaatttttg atgaaaccat aaagacagat cataaagtca taaccgaaga
     4741 agcctcaaaa tcaattctaa aatcctacgg ggtaaaggtt ccgccgtatg cacttgtgac
     4801 tacgccaaaa caggcagtca aggcggcaaa aaagattggc tatccactgg taatgaaaat
     4861 tgtttctccg caaattttgc acaagacaga tgttggcgga gtcaaagttg gcattgataa
     4921 tgacaaggat gtaaaaaaga cgtttaatga catgtacaaa cgactctcaa agaaaagagg
     4981 agtcgaagtc aaaggagttc ttttggaaaa aatggttcca aaaggagtcg agctaattgt
     5041 gggactccaa gtagacccgc agtttggtcc agcaatcatg gtgggcctag gcggggtcat
     5101 gaccgaagtc ttcaaagacg ttgcatttag aatgcttcca attacaacct cagacgcaaa
     5161 gtcaatgcta gacgaactca agggcgcaaa aattctcaag ggcttccgtg gaagcaaacc
     5221 aattgacatg gacatgcttg caaaggcgct agtccagatt ggcaagctgg gagtagagaa
     5281 cgcggattat gtcaacagca tcgactttaa cccagttgta gtatatccaa aatcatacta
     5341 tgtcgtagat gcaaaaatta ttcttggtaa tcaaattcac aaaaacgcaa tctcaaaggc
     5401 ccagccagac gattccttta tggaaaaatt cttcacgccg caatcagtcg cactaattgg
     5461 cgcgtctgca acaccgggca agataggcaa ctctgttttg gacagcctgg caaagcacga
     5521 ctacaagggc caagtgtatc caattaatcc aaaggcagaa gaaatcttgg gactaaagtg
     5581 ctatccatca cttgaggcaa taaacggccc aattgatctc gtcgttgtat gtgtagacct
     5641 ttctgttacg ccgccgattc ttgagagctg cgcaaagaag ggaattcaca atgttgtgat
     5701 tgtatctggc ggcggtaagg agcttggcgg agaaagagca gactatgaat ctcaaatcaa
     5761 agaacttgca ctaaagcaca aaatcagaat tattggtcca aactgcattg gaatgttcaa
     5821 tgcggcaaac agactcgact gcgcattcca aggacaggca aggatggtcc gcgcaaggct
     5881 tggaaatgtg gcactgctct cacagagcgg aactatggga attagcttcc tagagactgc
     5941 ggattccttt gggctatcaa agatggtaag ctacggcaac cgctcagatg tggatgaggc
     6001 agacatgatc tggtatctgg caagcgatcc tcaaacaaag gtaattgcgc tatatgtcga
     6061 aggctttgga gacggaagaa aattcgtcga gactgccaag cgggtaatga aggaaaagaa
     6121 aaagccagta gtgatatgga agagcggaag aacagaggcc ggagcaaagc aggcagcgtc
     6181 ccacacgggc tcccttggag gctcaaacgc aataatcatg ggcgcattca agcaggccgg
     6241 aatcatttca gttgacagct atcaggagct ggcagcagtt gtaaaggcac ttgcctggca
     6301 gccggcagca aagggcaacc gcgcagcatt gtgcagtaat ggtgcaggcc cgatgattgg
     6361 gggaattgat cactttgaaa gactcggcct tgagcttgcg caggtcacac cgcagacact
     6421 aaaagagctc aaagaacact ttccgcccac atatgttatt ggaaggggaa acccagttga
     6481 tgttaccggc ggcgcaaacg cagacgatta cagatataca attcaaaaat tctacgacga
     6541 cccaaacgtc gacattgtaa tgccgtggtt tgtcttccag gacgacccac tagaggagac
     6601 aataatccag tatttggccg agttctcaag gcagcaaaaa aagccactgc tagttggcgg
     6661 aaatggcggg ccgtacacgc aaaaagtctc agcactaatt gaaaaagaag gcgtgccagt
     6721 atacgatgat attcgaagct gggttgcagc ggcatcagcg ctagtccagt ggggcaaaca
     6781 tctctagaca acatttattt caagcaaaaa attttggatc aaacatggct ttagtgacaa
     6841 tctcaaaatc aaacggaatt gctacagtca aaatcaaccg tcctgacaag ctaaacgcaa
     6901 tgaatgtcga taccgcaaaa gaaatcatcc agatatttac agaattggac aaagacggcg
     6961 aagtcaaagt gataattctt accggcgagg gcgaaaaggc attttctgct ggggcagaca
     7021 ttgagtacat gtcaaaaatc agcgcagacg aatcagtgga atacgcaaag cttgggcagc
     7081 ttctcacggc aactgtggaa aacgtatccc aaccgacgat tgctgcagta aacggctttg
     7141 ccctaggcgg cggatgcgag ctggcaatgt cttgcgatat tagaattgca gcagacactg
     7201 caagacttgg gcagccggaa gtgacaattg gaataccacc gggatgggga ggaacccaaa
     7261 ggctcatgag aatagtagga attgcaaagg caaaggaact tgtctttact ggaaagatga
     7321 tcaaagccga cgaggcaaaa gaaatcggcc tagtcaacca ggtggttcca ctggcatcac
     7381 taatggatga gacattaaag atggcgcagc agattgcggc aaactctgtt tccggagtgc
     7441 gaatgtcaaa gattgcaata aacaagggac gaaacgcaga tctagatacg ggtcttggag
     7501 tggagctttt ggcgtggaga aactgcttta cagatcctga cagggctgag cgcatgactg
     7561 catttgtgaa caagtcaaaa aaataatcta acctaatctt ctttttattt acgtgggaat
     7621 tcggggaaaa ggttattgtg aacataccat cagcgagccg atggtctccc actactggga
     7681 tgtagttatg ttgccacagc tactcttcct gcttcatcga ccgagctttt gatctctagc
     7741 ccattttttg atggaccgtc ttgcaacgca ggcgtgcatt cccgcagttc ctgcggtaat
     7801 ctgtctgaat ccaatcccgt ttcggcttta catgacatcg ggtacagatt catatcgtgg
     7861 tcacgacctt cttccatttt gcctagctct aacattgggc atcttgcgaa ttgaccataa
     7921 ttaacaagct ggttatatca taattaccag gtttcaacaa caaacgaaca tttctgataa
     7981 gctgcaccta accttttttt atttcctagt gggtattcgt ggaaaacgtt cttataattt
     8041 agaagagcac ttgatcatat ggcaactgaa caatcacaga aactagtaac tataacgcca
     8101 aaagctgcag aaaagatcgt agagttcatg aaagaagagg cagaaaagcc agaatacctt
     8161 cgagtctatg tccaaggcgg cggatgctca ggactttcct atggaatggg ttttgaagca
     8221 aagccagaag aagacgacac agtaatcgaa gaggcaggag taaaactgct cgttgacagc
     8281 tacagccaag aacacctaca gggcgcaaac atcgactaca tcgagtcact gatgggttct
     8341 ggatttaaga tcaacaatcc aaacgtaacc aaatcctgct cgtgcggaca ctcttttagc
     8401 acagaataaa atctgcacaa tttttcattt ttcgatctcg tacccagccg gggctgtgct
     8461 gtgttaaaac tttgcataca cacttatatc tagatggcaa caaaatttag ctattggaat
     8521 ttaggaggaa tatattcaat tgccgccaac aggcattgtc aaatatcacg ttaagctatc
     8581 ctttgaagtt gatgggctgg tcgagcgagc agatataatc ggagcaatct tcgggcagac
     8641 agaaggactt ctgggcccag agatgaatct gaatgagctg cagcgcatgt ccaaagtggg
     8701 taggatagaa gttactacaa acactactac taataccacc aagggcgacg cacttatccc
     8761 gatgagcact gatgttgata ctgcctcact aattgctgcc gcaatagaaa gcattgacaa
     8821 agtagggccg tttgactgca agtttttgct tggtgcaata gaggatgtcc gcgcaacaaa
     8881 aaaagacgac atcatcaagc gcgcaaaaga aatcaaacag caatgggcta ctaaatcaat
     8941 tagcgaaagc gacacgatgc taaaagacat ccacgaaggc acatcatcgt ctggcaagtt
     9001 gacaacatac ggcaagggca agcttccgtg cggagtcggc gtctttgagt ccccatggat
     9061 tattctagtt gagggaagag cagacgtaat caatctgatc cgagcaggaa tcgacaacgc
     9121 ccttgcaata gatggtgcgc gaatagacga gtcaattaag gatttgtgcg acacaaaaga
     9181 aaaagtgatt gcatttttgg atggtgatag ggcaggtgga ttcatactaa aagagctaaa
     9241 aagcgtagtt gacattgatg tgatccaccg cgcaccagag ggtgtggaag tagaagagct
     9301 cacgccacag caaatcaccg atatccttaa ggacacaatt gatgaaataa aaaacccaaa
     9361 gccaaagcct acactttctg atccggaaga caagtcgata gcagagcttg cagaaaaaat
     9421 attttcgcaa atcaacgaaa cactagaggc aatcggatta gacgcaaaca gagccgaggt
     9481 cttcaaagtt ccaatatccg aactcgtatc aaagctctca gtgcagtctg gaataaaata
     9541 tctaatactt gatgggatag tgacgcaaag actagtggac ggtgcaaaac aggcaggcat
     9601 cgagtgtgtc atagggcaca ggattgccaa tctgacaaac caggacggaa taacgctaaa
     9661 gacatttacc gaccttggtg ttgcataaga caactaaatg tctgagctga aaatccaaca
     9721 cctagtgaca ctaactgagc tcctcacaaa gggggcaaaa aataattttg tttcaattac
     9781 cacctcatca cttgggaaaa acatccacaa gtcccagcag gccgcatcaa agcacctatt
     9841 ggagctagaa gaggccgggt tcatcgagag aataatgtct ggcagaaaaa tttctgtaaa
     9901 gctaacaaag aaaggatacg accagatatc agacgtgtta aacttgataa aaaaaggact
     9961 cgagtctata ccatcagtaa ttgagctgaa aggagtgttg attgccggaa tgggcgaagg
    10021 ggcgtactat atgtcgctta aaggctacac aaaacagttt aagacaaaaa ttggctatgt
    10081 cccgtttcct ggtactctga atgtcaagct tgacaaaaaa gaacacatcg aggccgtgcg
    10141 acagctgaat ggcattgagg gggtaaaaat cgaaggattc tccgacggaa agcgaacata
    10201 cggctgggtc aagtgccacc gctgcacgct aaatgacaga gtcgactgcc agctcatact
    10261 cctagagagg acgcaccatg ataattccgt aattgagctg atatcggaaa ctgatatccg
    10321 gaaaaaactc ggcctcaaaa acgggtctga gctgacaatc aaaattccga tttagaaatt
    10381 tggaaccaat ttggaagtaa tgtggaaatt tgctttaata gcatttggcg aatctggatg
    10441 caatgaacaa aaaaatgctg attgccgcaa tcattgtagg catcggagtt ccaataactg
    10501 tctatgcagc aagcccatta ttcattaaca cgacaataga tgagcctctt ccaactaaca
    10561 tcatgaagac agacgaaaaa atgatggaag aaaagatgga aaaagaaatg acggaggaaa
    10621 aagccatgct taaagatgaa atgatggagt ccgaagcaat gctggaagaa gaaatgacag
    10681 aagaaatgat aaaggaaaaa atgatggata aagaatctgt catggaaacg caaaccatgg
    10741 aagaagaaat gatggagaaa accatggaag aaaccatgat ggaaaaacaa accactcaat
    10801 caagtgactc agaagatttt tcagctaaat ccggagtctt tgtcggcgtt ggagatggaa
    10861 tacatgacgc gcaaggaaat gcgctagtca tcccactaga aaatggacaa aaagtacttc
    10921 gattggagga ctttcgctca actaacgggc ctgacctcta tgtgtatttg gcaacagact
    10981 ctggcgcatc agactatgtg agtcttggac gactaaaggc aaacattgga aaccaaaact
    11041 atgacatccc tgcaggaacc gacctatcaa aatacgacaa cgtgcttatt tggtgcaagc
    11101 agttttcagt actctttggt agtgccgagc tggcatagta tcttttttta tttttttata
    11161 ttccgtgcag ggcctcgccg tactcttttt caatttttga gcttgagaat tccggtattg
    11221 gggactggag tcttgctacc cgtacctcta gacccaagtt tttgcagccg tctgtgatga
    11281 atttttcctg gtgcacctgg tcgtacccaa gtgcgattat gtccggcttg acaacctcta
    11341 ctgtcttgaa gatgtcgcct tcgcgcccga ctagacacaa gtccaccatc gaaagcgagc
    11401 cgactagctc ctgtcggtgt tcttcagtgt ggagcggcgt tctttttttc atctttactg
    11461 ccgtgcttgc agttgccacc acaactacta ggacatcgcc tagcaccttt gcggcattaa
    11521 gggtgtggat gtggcctggg tggataatat caaagactcc gccggcaaga accacatgca
    11581 gtgcggttct gccaaactct gttagcgatt ttttgtcctt tgtaataaga tcgtttttgg
    11641 caagattatc catcctttca ctgaggtatt tttctgagag ctgcattctt ttgatgcata
    11701 tgtccattgg ctgctccttt gtgatattgc agacgtagaa tatgcctaga atttttttat
    11761 cgataatatc caaacaagga tcactacaca accattcttt ttaagccatc tatgaattta
    11821 gctcaggttc tatccctttt gctattctta gcgcgtcaac taggccgtcg gcgtatccta
    11881 tggacagtat ggcaagctcg tcttttccct gctcaaagaa ccgctcagca tccctgatgt
    11941 acagctctgc gttttcaagt actccttgga actctttttg gtccttgtag tggggtatta
    12001 tctgctcaag ggcccgtcgg accatcggga cgtatttttt catcatctgt gcggagattt
    12061 tttcaatttt ttccgaatta tctgctggct catctaggca ttctgccaat actttgaggg
    12121 catcagattc cgtaaagtga agcgcacctg ggatcacaat tgagtggggc ggtgcgccaa
    12181 aattcacatt agccagactg gatattttgc ctgaaatgat tttctggctt tcctgtccaa
    12241 ttcttgaggc aactattgca tacatggaat ccgaaattac ttttcttgtc tgctcttttt
    12301 ctgttgcaag caagctttga aatgcttctt ttggatccaa aaagaagttt ttgttttggt
    12361 tgtattctaa gagtataact gtgtggtttc caagcttgag gttctcaaaa acggtatagt
    12421 atgcagtagt tgacgggata ccgctcatta ttgtgactgt ctttcctacc ttgtagtagt
    12481 gaagaccgca ctcgcctact agtgacgtta gggcagacga tgcatgaact gtaccggtct
    12541 tgattttttc ctgtattgcc cgcgtcctaa gctcgatgtg cgttgttgca atgtatgggt
    12601 cgccaaagga aagaagggag atgttttttg attttgcatt ctctagtatt tcctttccgt
    12661 cctcgactag ccagcgtggg gcaaacttga attctccttt ggtcagctgc tttattttct
    12721 gtacgtcaga gtccggcatg gggctggtga actgttcaaa atataccaca tctgaatctg
    12781 aaattatttt ttttgtattc tcagaaaggg aatcaggacc agttatccct aacccgataa
    12841 accagagcat ctactttcct ttcttagaat cgtggagccg ctttaggtgg tgcagtgtgt
    12901 ttttgattac ctcgattccg cgcaggtact cattcatcga tactcgctca tccatggtat
    12961 gtgatgcgtg agggtctcca ggaccatatg tgacaacagg aatgttccag agtgtgccga
    13021 ttatgttcat gtctccagtc ccagtctttc taatcaggga tggccttgta tgctctacgt
    13081 ccattactcc aagcgtgaat gcgcgaatca ggggcgaatc cagcggcgcc tcaaatggct
    13141 ctgtctcatc taaaacagaa tagattgcct ctactccctg cttttttgaa atctcattta
    13201 ccagggttgc gattttttct ccgactgatt tgcagttcat ggttactggg atcctaatgt
    13261 ccattatggt gtcgcattct tttggcgtga cgttgtggct gtctcccccc cttatctcag
    13321 tgggcgttgc agtaaggatc atcccctttg attttccctc ctggttttcc tcaagggatt
    13381 ttctcagctc tgcagtaaac ttgagtgctt cttcgattgc gttttttgca agccacggcg
    13441 cagatgcatg tgcggaatca ccgacattaa ctttgaggtt gattgcaatt cttcccttgt
    13501 atgcgatagt aacatttcta attccgcttg gctcgccaaa gactgcatag tcgatgtcaa
    13561 gcttttgctt tgccagattt ttaattccgg ttgcgtttcc ctcctcgtct actgctccta
    13621 caaaaattat tgtgccgttg tttttttgca tagttgatgc cgcaagaagc attgccatta
    13681 gcggcgcctt tgcatcagat gctccgcgtc cgtacaaaaa gtctccctcc tttcggacct
    13741 ttaccttgcc cggcactgta tccatgtgtc cgcacagcaa aaccgtcggt gagccggagc
    13801 caattgtagc aatcagattt cctacctcgt ctatgcggat attatcaaat ccaagatcgt
    13861 cgcacttgtc tgccaaaaac tctgctagcg gcttttctgc tagcgacggc gtgtacagcc
    13921 gaagagcttt ttctaaaagc ttaattgaaa atcggggcgt aatagtaaat gacgagtcca
    13981 ttttttactt tcctgaatta actgcgtatt ctaaaatctt tgcggcaatg tctattccgg
    14041 tgactcgtgc cacgtttcta tactctgttg tgttgtttat ctcgtgtact acgaggcctt
    14101 tgtctttact ctccattaga tctactccga caatctggcc ttttactgcg tcttttgcct
    14161 ttatgcagag gtcttctagc tcctttgtca ccttgcagac ttcggcagtt ccgccaaggt
    14221 gggtgtttgt cttccagttt tcctcagtcg agtttctgta tatggctccc gctaccgtat
    14281 ccccaatcac tattgcacgg atatcgcgcg gaggcctttg cacaaactcc tcaagaaagt
    14341 ggacttggta aattgggtac attttttcgc gctcctcaat tatcccttcg gctgactcgc
    14401 ggttgtttag cttcgatatc atcctgcccc agctaccaat agtcggcttg attacccggg
    14461 gatatccaag cttgtctagt gcttctagtg ccgcatcctt tgagaatgcc actgtgacat
    14521 atggggtcgg gattttcatt ttttgcaaga gcatgtgtgt gaatagcttg tttccggcgt
    14581 atattccggt gtgcagcgag tttatcacat ttgcacccat cccttccagt gcggcagtgg
    14641 aatgcatgtt tctgtaatag ctgacgcacc gctgcagtac tgtgtggtat tctgtttttt
    14701 tatcaatgtc taggatcagg tttttacaat ctaccatttc gattttgatg cccgacttgg
    14761 ctgctgcctc gacgagggct ttttcttcta atcggatagt gtcgtaaaga accgttatag
    14821 aatgggtcac tgaccccagt cctctccaac gctttgggca ggctttagcc caaatccgtc
    14881 tgaagatttt attagctcaa aacttgctcc gcagtctggg caagttacca gttcgccctc
    14941 tagggcatcc tttggaatgg aaatgtctga atcgcattca gtgcattttg tcatggttat
    15001 tccttagtgc cctttgagtc attaattatc tttctttcaa gtttgctatc aagtggtatt
    15061 tctaggaggt ctcccatcct taggttcttg agcccaaggg ctactgcctt tgcctgctcg
    15121 tcgtcttttt ccagccctaa aatggacatt agatacgcag agcctgtctt gcctgcaagc
    15181 tcaccaaaga caatcctgcg cctgtttcct actactcgtg ggggtattgg ctcgtatgct
    15241 gctgggtttc gcagtatggc tgctaggtgc gttcctgcct tgtgcttgta tgccgaagag
    15301 ccaacaagtg gttttgaatc gtatggtgtg actccggtgt agctctcaat taatcttgaa
    15361 aggtccgata tcatgtccaa tcgaaaatca tttggtgatt tgtagaggta tgtcagtgca
    15421 actgctactt cagccaaagc tggaatccct gtcctctcgc caattccgtc aattgtagtg
    15481 tgaatttggt ctgctcctgc atctactcct gcaaacgcgt ttgccagtgc aaagccgatg
    15541 tcgttgtggc agtgcacatc aagtggtact ttgacttttt cgcggattga ttttacaaaa
    15601 ttgtacattc cctttggcct catcacgccg acagtatctg gcaggctgat tctgtcaact
    15661 cctgcctcct ctattgcctt gcacaccttg agcagaaact ctgggtctgc cctgcttccg
    15721 tcttctactg taaatctcat ttttagtccg tgtgactttg catactctac tgtctctact
    15781 gcccgtcgca gagcctcctc tcgtgttatt cggagcttgt ccttcatgtg gatgtctgaa
    15841 attccaaggt atgttgcagt ccactttggg tcgcacttta acgcaatgtc gacatctgac
    15901 ttgagggccc tcacatgtgc tacaatgtct gcctttagtc cctgtttgat tatggtttta
    15961 gttgcctcga ggtggtcctc agatactact ggggagatct caatttggtc cacgccaaaa
    16021 taatcaagca tccatgcgat ctggattctc tgcttgttgg taaaggtgac acctgggtgc
    16081 tgctctcctt cccgcagcgt gctgtctagt acgcggatct tttttggctt tttttcatac
    16141 tcgttgtaca gatgcgcata atagtttgga tcactcattg cagaatcagt taatccacgt
    16201 ggtggttata aacatattcg tactaaaaac gtcgtaaatc agcaaaagtg tgtatttttt
    16261 aggcttaaag cagagatttg agctcgattc tcgtcttagg ttatttcacg caggataatt
    16321 accgtgtttg tgtctgagac gcccgggacc tttcggagag aatctattgc ggcgtttatc
    16381 tcagttatgt ttggagacga tattattact gcaatgtcgt actggccggt tatctcgtag
    16441 actgtcttta ccccctctag ttttgtgagc cttgctgaga cctttgatgt gtcagttgac
    16501 gactctactg atattaggac gattgcctta gtagagtttt gctcgccgat ttctattgtg
    16561 aattttttta tgatctttgc gtcagagaga ttctttactc tgcgccttac tgcggattct
    16621 gagagcttta gcttttttcc aatgtctaca aatgactccc tggaatcctc ctttaggatt
    16681 tttagtatct cgtcgtcaat tttgtccttg tacattcttt ctcctctctt cctctctggt
    16741 aaataacttg tctagtacct ctaatacttt tgtaatgtcg gcctctgaga gaaccagcgg
    16801 cggcaggaac cgaagtatgt ttctgccaga gtatagcagc aatactcctt ctgaaatccc
    16861 gtcaaataaa atgtccttta cctcgtactt catttctact ccgatcatta ggcccttgcc
    16921 cctaacgtct cttatgatgc tgtgtttttc ttttagattc tctagacctt ctttgaattt
    16981 tttgccgatg gttacggcat tttggattaa tccgtcttgg gttaaagctt gcagtgtggc
    17041 aattcctgcc gcacacgata tggggtttcc gccaaatgtg gacgagtgct cacccttgct
    17101 cattgcggcc aagacttccg gcttgactag tgtggcagaa attggaatgc ctcctgcaag
    17161 tcccttggca agacacatta tgtctggtgc cgtattccag tgctcgcacg cccacatttt
    17221 gcctgttctg cctagtcctg cctgaatttc gtcaaaaatt agcagaattc cgcgctcatc
    17281 gcagagcttg cggacatctt gcaaaaatcc gtccggtgcc acattaatgc cactctcacc
    17341 ctggattggc tccattatta caaatgcagt gtcggagtct atttttgatc gtagtgattc
    17401 tatgtctcca aaaggcgaga atgtcacctt ttccacaagc ggtgcaaatg attttctata
    17461 ctttggatta aatgtcaaag acagtgcacc catggacttg ccgtggtacg agccgttcat
    17521 tgcaatcatt cctgattttc ctgtgaactt tcttgcgaat tttattgcgg cctctactgc
    17581 ctctgctcca ctgttgccca ggtgtacctg tgatagtccc tttggtgctg ccttgattag
    17641 attttccagg aattcctctc ttgtcttatt atacagagat gtgtgtactg tgattatttt
    17701 gtcaagctgt ttttttattg cctcgactac tcgggggttt cggtgaccta ctagtgctac
    17761 cccataccca cccatgcagt cgatgtattc tttgttgttt atgtcccaga ctcgggtccc
    17821 tagtcctctt tctattgtta ctgggaatct ctggtacagg tttcccaaaa agtcgtcttc
    17881 tgtcatcttg tgattaccgt gcactttgtg tgagatattg ccgatgatat aggattttcc
    17941 cgctggccgt ttgcaattag tgcctcactt acccccatgt ctagggcctc agaggctgca
    18001 agtattttct tttccatccc aaagccgacc tttggcctaa tctcctttgc ctctgcgtta
    18061 gtcatctttt ctacgagctt gtcgttcatc aaaaggccgt cgacgtttgt caaaaacagt
    18121 attttatcgg actttgttgc gcctgccacg tttgctgcag ccctgtcgcc atcgatgtta
    18181 agaaagtcaa actctttgga tatggcaatc ggggaaatga caggtgtgta gccttggtcc
    18241 aaaagaagct ttagcaggga cgcatttacg cttgtgacct tgcctgtata tcctccgtca
    18301 attacctgct ttcggccttt ttcattaatt attaccagct tgtcctttcg ctcagccaaa
    18361 aagatctgcc cgtcgactcc gctgagtccg actgcgttta ttccgttttt ttgcagcatc
    18421 tgaactatgt tcttgtttat tttgccggac attaccattg tgaaaatttc cgcagtctcc
    18481 ttgtccgtgt atcgtgattt gattccgcta ggtgatacta caaattttgg ttcttttccc
    18541 aataattcgc aagtcttagt tacctccttt cctccaccat gcactagtat gactccttca
    18601 gattgtgcaa ctttttttat gtcaggaatc gtagatggat gaagattttc tactacgctt
    18661 ccaccaattt tgattgtgat catttcttac actggagtca gaggcgagta cctaagccca
    18721 tccatttcgt caaagcctga catgacgttc atgttctgaa tggcagagcc tgcagcaccc
    18781 ttcatcaaat tatcagaagc tgacagagca actagtctgt ggttgtcctc gtctatgtca
    18841 aacccaatgt cgcaaaagtt agagccgact acaaacttgg ggtctgggaa tttatacaat
    18901 ccctttttgt cccggattag cctgatgaat ttttcgtcct tgtacatctc gcgataaacc
    18961 ttccagagtt ccagctccgt catgtctttt agcatgaatg tgtggtttgt gcagaggatt
    19021 ccccgaacta tgtccactgc gtgcgggctc atcgatacat ggattggctt tcctgcaatc
    19081 tctgatagct cctgctctat ctcacctgtg tgcctgtgct ttgccggctt gtatggccta
    19141 attacacctg agcgcattgc gtggtgtgtt cctgcaacgc tcccggcacc agcacctgat
    19201 gagccgattt ttgaatcaac tattatatgc tcagtatcga tgagattgtt tttaattaac
    19261 ggcgcaaggg ccaaaataga agttactgcc atgcagccag gacacgagac cagctttgca
    19321 tctttaattt tttctcggtg taattctgga atgccaaaca ctgactttgc cagatagtca
    19381 ggatgcggat gctcccatcc gtaccacttg tcataccctg cctggttgtg cagtctgtaa
    19441 tctgccgata aatcaactac ctttaaacct ctatcgtata atgccttgac aatttctgtt
    19501 gcagttccgt gcgggactgc ggtaaacacc aggtcacact tgtcagacat tttatcatag
    19561 tctaattccg agaatgtcat ctctgtgaat cccttcagtg aaggctggat tcggtgcaga
    19621 tattctccga cgtactgtct cgatgtgacc attgcaattt cgactttggg atgtgatact
    19681 aaaagacgca gcatctcgcc tccgacaaat ccagatgcgc caaccacacc tactcgtaac
    19741 atatcatttc cctcgcgcag tgctataatt tattttgtga tgtgggttat agcaaacttt
    19801 accatttctt gtggaatgtt cttttttgca acctttgcca gccccttgaa ctcgactgtg
    19861 ttatttacct cgtgtactac cagtccgcgc tttttgtcct ccatcagatc tattccaagg
    19921 attcctccgc caactgcctt ggatgccttg atgcacaagt cttctaactc ctttgttatt
    19981 tggcacagct ccggctctgc gcctagtgca atgtttgtct taaagtcgcc ttcggacttg
    20041 cggtacattg ctgcgacaac ctggtctcct actgtgattg cgcggatatc acgaggcggc
    20101 ctgtcgatca tttcctgcag atagtaaatt ctgtcgtgcg agccgtcctc aatatcacgc
    20161 atctcaatta ttgcctcagt tgtgtcctta tcacgcagtg gtaagattcc tcggccccag
    20221 cttccaatta ctggctttat gactagggga tagcctacct tttcgattag ctttgttgct
    20281 tcttctgatg aaaatgcaaa gtatgtcctc ggtgttggga ctccggcctt tcttagcaaa
    20341 agcgatgtga tcattttatt gccgcatcta tccgatacgt caaagctgtt gattacagga
    20401 acccctagga attccagtgc tgcagtaaag tgcaggccgc gaaaataact aacgcacctc
    20461 tctagtacga catcaccaaa atcaaagtcg gagcgtacgc tgtctgtgtt tagctgagtt
    20521 atttttgcgt caacctttgt cgtgttatgg cctagctttg atgcctcgtc ttcgagcatc
    20581 ttttcttctg gtctgacacg gtcaaagaca atacaaatct ttgacaacta ttctccccag
    20641 tcttcgccta cggtttctgc tggttttagc tggatagttg ctccttcttt ttgtgcaatc
    20701 tcaaagtctg cgccgcattc ggggcatgat actatctcac ccacagatgc atcgtctggg
    20761 acgttgatac ctgcatcaca ttctgaacat ttcatggtaa cgatttttct cacctcttcc
    20821 ttagtaattt attcgcttcc gtcgtctgca gtccccacaa ctccacaaag cctgccgcct
    20881 ttttctgatc aaacgcagag cccttgccgt atgtggccat atggtggctg tacaaagaat
    20941 actttgattt tcgtccgacg acgcgcaagc ctcccttgta gagctttatc ttcactgtgc
    21001 cactcaccac cttttgtgag ctgtcgatga atttgtccaa gtctgccctt agtgggtcct
    21061 gccagagtcc tgaatatgca agccaggccc actctgcgtc tgccaaatcc ttgaacttgg
    21121 tttggtgctt tgtgtgtacc attttttcca aatctgaatg agcttctata atgcaaagag
    21181 ctgccggagt ctcgtatact tcgcgcgact ttattccgac tacacgatcc tctatatgat
    21241 caactattcc tacgccgcaa gagcctgcta ccttgtttgt atattcgatt agcttaattg
    21301 ggtccatcct ttttttgtcg actgcaactg gaatgccatt tgcaaactcg atttccagat
    21361 actttggcga atctggcagg ttctttgtct taacccagat gaatgcgtct gcagggggct
    21421 cactgtatgg gtcctcgagg tttccgcctt cgattgcccg tccccagagg ttctggtcaa
    21481 ttgagaattt ctttgaaacg ttttcaactg gaatcttgtg cttttttgcg aattttagct
    21541 cagttaccct gttcaagttt aggtcgcgaa tcggtgcaat gattggcagg ttagaaccgg
    21601 atgcaaatgt cacatcgaat cgcacttggt cgtttccttt gcctgtgcag ccgtgtgcca
    21661 gtgcatttac cttttctttt tttgctattt ctagtacctt ttgcgcaatt agcggccggg
    21721 ccaaagacgt tgcaaggcag tacttttttt ggtacatggc gtttgccttg attgccggat
    21781 ggatgaaatc tgtgacaaat tccttttttg catcaatatt gtaatgtcgc aaaacgccta
    21841 gcttcttagc ttttgctgca attttgcgct ggtcgcttcc ctcaccgaca tccactgtta
    21901 ctgtaattac gtccatgtcg tgcttttcct gcaggtatcg taccacaaca gatgtgtcta
    21961 gtccgccaga aaaagcaaga actgcctttt gtttcatcag agctttttgg aaatgtgatt
    22021 tgcttttatc ttttgtgttg atctggtttt ttctctatac tttcttgaaa acttctagtg
    22081 tggtctcaat tttacactcc aaattggcat gacatctatc tggagatgga ttgccctctc
    22141 atcaaaattg ccgaatcctt ataatggatt cttactctat aagatatgga aacaacaatg
    22201 aaatttacca acaatgtggg taagcgcaaa tttacaatgc tggtgcaaga agaacctgaa
    22261 ggtggtttta ctggaaaatg ccttgagctg cgtggcgcaa tcagttacgg caaaaccatg
    22321 aaagaattaa aagctaacat gaaggatgct atcgctctta ttcttgatac attggaagaa
    22381 gaagcagaga aaggcaacta tcgaaaggaa gtaatcgaga tttcaagctg aaatgaattt
    22441 ttgaatgagc ctaaagaacc acgactggcg taaaatagta aaaacgctaa gccattttgg
    22501 ttatgttcca gtaagacagt ctgggggcca cataattttg agaaattcaa aaggtatgat
    22561 agtttcggtt ccacgatatg atccgctgcc tgaaggaaca aagagcaatt ctgttagaag
    22621 cagaaatcaa aaaagaggat ttcataaaaa agttgtagta aacgaaaagt gtgattttta
    22681 ggaaattttt cattatcgag ttttggaaaa aatctttttg aggcaaaatt tccaaactag
    22741 cttaagctaa aatccgaaca ctatttaacc aaaatttcat aacggtgttt tatgaaaatc
    22801 cgtctcctag ttggcattgc agctgcggca atagttctat cggccatagt actggcgggt
    22861 actgcggcaa aaacctcgga ccaaaacaag attcgagtcg catttttccc aaacatctct
    22921 catgcagtac caattgtagg cacagaaaac ggcgagtttg ccaaagaact aggagatatc
    22981 ccaatccaga cagtaattgt ggattccggc ccgcaggcaa ttgaggcgct ctttgcaaac
    23041 tctgtagaca ttgcatatgt ggggccctcg cctttcatca acggatatgt aaagtcgggg
    23101 cagggaatta agatcatatc tggtgctgca aacaacggcg caagcctcat catacaaaaa
    23161 gactcggaaa tatctggaat atctgatctg gctggaaaaa aaatcgcagc cccatccatt
    23221 ggaaacaccc aggatgtctc actgcggcat tacctgtctg aatatgacct aaggcctgca
    23281 gaaaggggtg gaagcgtcat agtatacaac actgccaact ctgagacctt tactctgttt
    23341 gccaaaggtg acattgacgc agcatgggtg ccagagccta ctgcaaccat actagttgag
    23401 cagcttgggg gaaaacgcct gttcaatgag gaggagcttt ggccaaacaa acaatttcca
    23461 tccgttttgt taattggaag aacggatttt attgaaaaac acccgggctg gattaagagt
    23521 tggttggcag cacatcaaaa caccattaac tggattaacc aaaacccgga aaaaacggag
    23581 gcagtcttta tcgattttta caaaaagcac acaggcaagg ttttgcctga gaatatagtc
    23641 cacggctcat tttcacacat cgtatttaca tcagatctag acgaggtggc aatgatgact
    23701 tttgctgcgc gtgcatacga gcttgggtat ctgggccgct cacaatacaa cattgatgat
    23761 atcttttaca attccacgga ggacatatca tggcaaaact agaggcaaaa aacattatca
    23821 agcattttga tcacgacggc cagaagctaa tagcgcttgg cggagtaaac ctcacagtag
    23881 aggatggcga ctttgtgtgt ctggttggcc catcggggtg cggcaagtcg acatttctga
    23941 gaatcgcggc aggactgcaa aaaccagacg aaggccagat actgcttgat aatcgtccga
    24001 taaacagcac aggccctgac aggattctag tattccagga aggtgcgctg tttccatggc
    24061 tgaaggtccg cgacaacgta gagtttgggc taaagatagc tggaattcca gaatcagagc
    24121 gccaccaaat atctgacaga tatctggaga tgatgcagct gaccaagttt gcaaactcgt
    24181 acacgtacca gctatcaact gggatgaagc agcgtgtttc cattgctcgg gcgctagtga
    24241 tggatccggc agtattgctc atggatgagc cgtttgcatc cctggat
//
LOCUS       DASWKH010000082        17242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2078307, whole genome shotgun sequence.
ACCESSION   DASWKH010000082 DASWKH010000000
VERSION     DASWKH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 17242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17242
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078307"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VGA92_06340"
     CDS             <1..421
                     /locus_tag="VGA92_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9846068.1"
                     /translation="RNRKIMRSTKIVTSFVTNSQKFLILKRSKRVKSMKGLWGAISGI
                     IEAGEEPLERAKIEIFEEIGANNNAVRLLKAAKEMIISSTQYPDHQWIVYPFLFALSS
                     AKITLNWENESYKWIAPDEIYHYKTVPSLDVVLFNLL"
     gene            complement(404..1732)
                     /gene="thiC"
                     /locus_tag="VGA92_06345"
     CDS             complement(404..1732)
                     /gene="thiC"
                     /locus_tag="VGA92_06345"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX9846069.1"
                     /translation="MGTQFSAARRGVITDEMKQVARDEAVTEEFLRKAIASGSIIIPA
                     NNSRPQKVHRVGIGKGLKTKVNVNIGTSTLKVDLNEEIEKAKIAVKHHADTIMDLSDG
                     GDVGSIRRALLEAAPITFGTVPIYEAYNYGVEKHKNPLDITEDDYLNAFENNAKDGVD
                     YTTIHSGISKEMAKRILKVQRYGGVVSKGGTITAAWMLKYDKENPYISHYDYLIEIAK
                     KYDVTFSLGDALRPGSILDSHDELQVQEMINISRLVKQAHEHDVQVMVEGPGHVPLNE
                     VAANVRLAKSLIGDVPYYVLGPLVTDIASGYDHISSAIGAAIAASEGVDLLCYLTPAE
                     HLALPNAQEVRAGLIAYRIAAHAGDLVKLREKAIIWDMKMTEARRTLDWEKQIALSID
                     PEEAARIHNRTGQRDGNNVPCTMCGGACVYVMLPQQRKYTKETENLQQVE"
     gene            1870..3177
                     /gene="thiD"
                     /locus_tag="VGA92_06350"
     CDS             1870..3177
                     /gene="thiD"
                     /locus_tag="VGA92_06350"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX9846070.1"
                     /translation="MNILSIGGSDPSSGAGIQSDIRAASALGANCFSVITAITSQNST
                     RFSSVEAVSANAVAQQIDSVLSDFGIDVINIGMVYDSKIIEEIYLKLKDQKVPIVVDP
                     VIKSTTGGVLLKKSALFAFKRLIIPLSYAVTPNVKEAEILSGVRIAGFEDLIRAAKKL
                     SHLGAKNIVITGHAFTKNKISDFVYEKGKHHSISGKKLGGWAHGGGCNFAIALSYSIA
                     QKKSVAESVRFAKEFAYQSIKSAQDLGHGIKITRPKPDKIRTELAFAIHEFTKLDGVT
                     CLIPEVQTNFVFAKQNAKSIDGILGVGGRIVKAGKTAIVAGELEYGGSHHVGSAVLTI
                     QKKFPHLRSAVNIRFDERFIKKMQKAKYTILSYNRAGEPKKTRLKENSTISWGIQSAI
                     KNSAIPPDAIYHRGDVGKEPMIIVFGESPRDVLAKVKILLRFK"
     gene            3221..3922
                     /locus_tag="VGA92_06355"
     CDS             3221..3922
                     /locus_tag="VGA92_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9846071.1"
                     /translation="MKAVILAGGLGTRLQPYTTFLPKPMLPLGEKPLLEHLIAWVKRN
                     KIKEIVLCVSYLKKTIEDYFEDGNRFGVKIEYAVADRPLATAGQLKTAEPFVDDTFVC
                     VYGDSVFSFNLAKMINQHKTKKSFITMSLRQYNTTLKYGVIETTKSGKVTAWNEKPPI
                     SANINIGCYVMEPGVFGLIPAGKPYGMDDVIKKALAKKKDVNSFLIKNGFIDIGDKIS
                     YKKAYQEFREKLGKI"
     gene            3922..4356
                     /locus_tag="VGA92_06360"
     CDS             3922..4356
                     /locus_tag="VGA92_06360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9846072.1"
                     /translation="MSEITIRKIQESDIDCGFLESLDSLRKASDLDKKKAKEILRKIA
                     ANPTHVIFVALNENRVIGATTLLIEQKFIHGGGLVGHIEDVVVSKEQQGLGIGEKIML
                     AVLEYAKGQGCYKTILDCDDNVKPFYEKIGFKRHSNGMRYDH"
     gene            complement(4353..4772)
                     /locus_tag="VGA92_06365"
     CDS             complement(4353..4772)
                     /locus_tag="VGA92_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846073.1"
                     /translation="MAIEKYLAVASLGLFAMFCGEINVFYNYLIAPRVEVEPAAQILM
                     FISIGAAPAVIIAGTAFLMTKRYGSKQVGSIIIAGGAVLFVGMLYANTLVPQIDRNYL
                     VYAVEIIPPLFMAVSFAVMGVGGLLFRTKKRPIKEYF"
     gene            complement(4827..5843)
                     /locus_tag="VGA92_06370"
     CDS             complement(4827..5843)
                     /locus_tag="VGA92_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9846074.1"
                     /translation="MGSMRKDYLSERFVIVSQNDEKESESKKCALCPGNESMTNPSLL
                     SLVAKDGMLQRLQDSEDNYVRNWSVRVFESKVPAVTISAENSYSDRPLYSEPAYGYHY
                     VVVASEKHKDSLATIPVEQWSNVLVVIQDRMRWLYTQRGVTYVSIYINHGKEAGSITP
                     HPHINMVTFSTIPPLIEQEAEASHRFLNEKGSCPLCQTVNTETGGPRQVLQTEGFLAF
                     CPWAPSYPFEYWICPKKHTTNFSKITQKEINDLSLILRATLGGLSNSLKNISYNLVFH
                     LSPEKKNSRQIHWHIEIYPMTSAWSGLERGYGVFINSIPPEKAAEQLGAACRKELAGL
                     VGIT"
     gene            5991..7247
                     /locus_tag="VGA92_06375"
     CDS             5991..7247
                     /locus_tag="VGA92_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9846075.1"
                     /translation="MVYVLLDGVGDLPHPDLKGATPLDYAKTPNLDQLAKNGAMGEVI
                     SVGKGIAPESDIAVFNMLGYKFQHSEYAGRGVIEAIGTGIDFRDGDLALRGNFATIDD
                     GGIIIDRRAGRKIEREDAIAVAKEIEGQIKFSEPGASVVVSPTIGHRVVVRIRCDGKA
                     LSSEITNTDPAYSRVDGMGIAKAVGDYMKIERCLPLKDIPNASLTANLVNEFTEQSLK
                     IMRVSQTNQKRKQQGKKLLNSILLRDAGNHYPKAVPINELYSLRFSCIVDMPVEIGIA
                     EILKMKTFSAGGLTDYEKKAQVAAKAMETENAIYVHLKGPDEFGHDGDALGKMKNIEE
                     IDRRFFGTLLYNIDTSKVAVVVSADHSTPCINKGHSDDPVPVLVSADSVKKDNSERFT
                     EKNAKVGSIGLLEGAQVLKTALQMIK"
     gene            complement(7240..7800)
                     /locus_tag="VGA92_06380"
     CDS             complement(7240..7800)
                     /locus_tag="VGA92_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX9846076.1"
                     /translation="MDRFMLHLKNTGYTPQDTGKLLLKARQLTSGMNVIIRDTRASTK
                     YVEFDVSVDKSKIDTLSEILAPIAPLDHARHVIEEHIEKEEAIKMGISYFNDERFWEA
                     HEVLEGVWKKCYEGEKELVQGIILAAAAMVHYQKAEDDICLSVLGRAQTKLARAAGMY
                     HGINIDALRQKIQEIKNSAQISLFTI"
     gene            7885..8301
                     /locus_tag="VGA92_06385"
     CDS             7885..8301
                     /locus_tag="VGA92_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846077.1"
                     /translation="MSMIETINDVNNTFLSRREITCNFKGVAGKLKKLEAAEIISKQF
                     KLDGKVVIPIFMKAQSGRPDVTGTFYVYDDEVLAKKQVNEVIFKRLEKAKGEAAAATE
                     KAAEAEKAEAEKVVETEKVVEASAPAETESKEEVKE"
     gene            8301..8480
                     /locus_tag="VGA92_06390"
     CDS             8301..8480
                     /locus_tag="VGA92_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HEX9846078.1"
                     /translation="MAGKKAAAKATGIAAYYKISGDKATRTHKICSRCGKGVFMSEHK
                     DRRTCGKCNLTEFIQ"
     gene            complement(8509..9342)
                     /locus_tag="VGA92_06395"
     CDS             complement(8509..9342)
                     /locus_tag="VGA92_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEX9846079.1"
                     /translation="MGLDSAIEWTEATWNPTTGCTKISPGCANCYAERLTKRLQMMGV
                     KKYERGFQFVQHNDDLYLPLTWRKSKKIFVNSMSDLFHEKSDFEFVKKCFFSMIMANW
                     HTYQILTKRPQRMARFSNLFETEFKIKIPHHIWMGTSIESEHYNWRIDELRRVRCHAR
                     FISFEPLIGDVGQINLEGVDWIIIGGESGYGFRQVKKDWIKNIVKQCKQQDVAVFFKQ
                     WGGIRPKSGGRELDGKTFDEYPQIQVSEQTKSDELALKKKIEQIAKQEQTMLIPKLLN
                     R"
     gene            9479..10408
                     /gene="tcmP"
                     /locus_tag="VGA92_06400"
     CDS             9479..10408
                     /gene="tcmP"
                     /locus_tag="VGA92_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEX9846080.1"
                     /translation="MARDPYLWAKEKISFLLEKTDIIKNDGLKKSPGNIWSIKKLLAL
                     DYYIGGSHVLFKKNFTNWHYVDTHCGSGLIGFEKEKLQLERFPGSPLIAALRANNQPF
                     TDYHFSDIDGESINSLSQRLQKMKLQVGNKDYSPMVRSFEATADHVTRYKKWGNAFLI
                     FIDPVGFTELKWSVMKKLLEIKTADIFLTFMTSFIALNRTNAHQGTEYEKTFDEVYGV
                     PDWRKEIGVDALLELYLNQIRKFRNYVYTIPVHRAGENRLYDIIVATNSRGAGSIIDY
                     TKKIMEILTTELIEAALKVTTKKDRDLDEWFDG"
     gene            complement(10431..11138)
                     /locus_tag="VGA92_06405"
     CDS             complement(10431..11138)
                     /locus_tag="VGA92_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846081.1"
                     /translation="MDHLVKVREAHQKGVIPQKIFDTISQRFPLVTEGIQRIEKASGT
                     PYPISYVEPSAIVTGSDSMSFQYGILFARTLPVFFEEKFQVVIQISAPLVAFGLKGTI
                     HAILAHEFLHYIELIRKISTMEIVSDEVSANLFENVYADSTRLFEPRAVFDDKTLLTH
                     ITKKFPAGFKDYKLEDKTEKLWIAKHLPVTNISLDTNTVKLSAEMLAKVSLDPILLNK
                     IKEYEERESKLRKKKLY"
     gene            complement(11171..12328)
                     /locus_tag="VGA92_06410"
     CDS             complement(11171..12328)
                     /locus_tag="VGA92_06410"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HEX9846082.1"
                     /translation="MRQKPLVAVLESSKKILHEYDLCDYCLGRLFAKKLGLSSNKKLG
                     EKIHKKLKIKTTKCYVCKNIFDNVPVYVAKMQEASSEYGFQTFLVGAKLKPSILDRDD
                     HLRSQFKLRGMEGVKTNVTRQLAKQFARKTKKKGQQLDPDITFTINFKEESCDVHSKP
                     VFLSGRYTKSDRNTPQKQKPCSNCLGKGCSSCNYHGISEYDSVEGAMSEYFFEKFGAT
                     QVKITWIGGEDSTSLVLGSGRPFFAKLIGPKKRNLTLPKKIPLGKIIVFSLKQIKKIP
                     TMPVRFVSKTRLLITTEQPIQPESLSKLLQLKDTIAVYEKSGKRAEKSIYDVKYKINS
                     ENSFYLSLVIDGGVPLKRLVSGEDVFPNVSDILENKSKCETFDFEQVKITN"
     gene            12487..13671
                     /locus_tag="VGA92_06415"
     CDS             12487..13671
                     /locus_tag="VGA92_06415"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9846083.1"
                     /translation="MPLKSIRQNHTPNSEQTRMMEIFRQMVNECIRIGLENNCTTLKK
                     LSSFSYSKLQNYDILSYYKLTAISQACGRLIQRKKDIKKGKKPKIPYVEKLFLVSCYG
                     FKINGMLLSFPVRNREFANILLNDYVARNISDTSLKIRSFTMTPESLSLSIQKEVEPI
                     KPEKTIGIDRNLRNITFGNDDKVILVNTAELLKIKENYTYIKSTFRRNDHRIKKKIVG
                     KFGIRQARRIQQRIHKISKSLVEYSKKQKAMIVFEDLKGIQKLYRKGNGQGRKFRRKL
                     NSLSNYELERQTTYKADWVGVPVCHVNPRGTSKLCPRCGGRLQEDRQRRRDLWCGICE
                     KWQDRDVVAAMNISRKGLARLTNPQGDTNEAMRGNLISDSDQEPVILRVDVSKLITEL
                     VL"
     gene            complement(13699..15027)
                     /locus_tag="VGA92_06420"
     CDS             complement(13699..15027)
                     /locus_tag="VGA92_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX9846084.1"
                     /translation="MLDNLKNSLQAAIKKIVKSSGVDEELIKELAKDVQRALLQADVN
                     VKLVLEITKNLEYRSINEKPPPGLSRKDHIVKILYDELANLLGKENEFSFKPGKLNKV
                     LLLGIQGSGKTTIAGKLAKFLTGQGYKVGVIGADTYRPGALVQLKTNCEKAHAEVYGE
                     ENNKDATQIVKNGLKHYESSGLDIILIDTAGRHKEEKELLDEMKEISKVAAPDLVLLV
                     IDGTIGQQCYSQAESFHKTVPIGGIIITKLDSSAKGGGALAASAATGAKVMYVGTGER
                     IDDLELFSPTRFVGRLLGMGDIQALLDMAKRLESEADEVRLKRISSGKMNMDDFYYQL
                     EEVTKVGSLRGFLDNMPGLSGMVKEDQLDQMEDRIGRWRYIIQSMNKQEKADPDGLLN
                     ASRIKRIARGSGWPEHEVKELLKNYKNSKSMMKASKGRQMQGFLRKMGLG"
     gene            complement(15021..15710)
                     /locus_tag="VGA92_06425"
     CDS             complement(15021..15710)
                     /locus_tag="VGA92_06425"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEX9846085.1"
                     /translation="MNLAALFSGGKDSTFSIYKAQKLGHTVSCLVTILTSSEESHLLH
                     HPNIELSILQAKSMNIPQILVRADSVDTKKEVELLKAALEKAKNDYNIEGIVHGGILS
                     EFQKTQFENIAKSLGLQILSPLWKTDQKQYMQELLDSGFEFIITAVSCDGLDDSWLGK
                     KITKKDLDELILLSEKHKFNLSFEGGEAETFVISCPLFCGAIKIHDSRKEWDGYRGRF
                     EIVQAKIEKQC"
     gene            complement(15819..16229)
                     /locus_tag="VGA92_06430"
     CDS             complement(15819..16229)
                     /locus_tag="VGA92_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HEX9846086.1"
                     /translation="MSKPSDLGSLKIGSYILLPVGDQATGEPCRIVEYDTSKPGKHGA
                     AKARIVGVGVFDNQKRPHVGPVSMQVHVPLIDKRSGQIISITGDQVQVMDSETFETID
                     AQMIDDEVKGKLEQGQMVEYWKVMDRTKIMRIKS"
     gene            complement(16289..16891)
                     /locus_tag="VGA92_06435"
     CDS             complement(16289..16891)
                     /locus_tag="VGA92_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846087.1"
                     /translation="MSETSLDGYACVPYTHDHKSIEIKDSWQDSKNIENMYFVTATFA
                     QDSKPYFPSHANHYLLVKYANNNIVKDIQRYQQSKTSFVFNTNSEIFERQVDGTMNFV
                     SVYYLEYSKSDEDLSDLAMVVSKNDHVRKASTGNMLTVAADPPKFTFPYSNNIVVLEV
                     SSQKSHQSVNKYCEQTRRNVCRKGITMNNLVGLSILEKLK"
     gene            17011..17130
                     /locus_tag="VGA92_06440"
     tRNA            join(17011..17048,17095..17130)
                     /locus_tag="VGA92_06440"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17045..17047,aa:Tyr,seq:gta)
BASE COUNT         4818 a         3691 c         3701 g         5032 t
ORIGIN      
        1 gcgcaatcga aaaataatgc gttcaacaaa aatagtaacc tcttttgtga caaacagcca
       61 aaaattcctc atactcaagc ggagcaagag ggtaaaaagc atgaagggtt tgtggggtgc
      121 aattagtggc ataatcgagg caggggagga gccacttgag cgggcaaaaa ttgagatctt
      181 cgaagaaatt ggtgcaaaca ataacgcagt gaggctgctc aaagcggcaa aggagatgat
      241 aatttcttcg acccagtatc ccgatcacca gtggatagtt tacccatttt tgtttgcgct
      301 aagctcggca aaaattacgc tgaactggga aaacgagtca tacaagtgga ttgcgccaga
      361 tgagatttac cactacaaga cagtcccaag ccttgatgtg gtgttattca acttgctgta
      421 ggttttcggt ttcttttgtg tactttcttt gctgcggcag catcacgtac acacatgcac
      481 caccacacat ggtgcaaggg acattgttcc catccctctg accagtcctg ttgtggattc
      541 gagctgcttc ttctgggtca attgaaagtg caatttgttt ttcccagtct agtgtgcgcc
      601 ttgcttcagt catcttcatg tcccatatga ttgccttttc tctgagcttt acgaggtcgc
      661 ctgcatgtgc tgcaatccta tatgcaatca gtcccgccct gacttcttgc gcgtttggca
      721 gggcaaggtg ttctgcaggt gtaagatagc acaacaaatc aacaccctcg cttgcggcaa
      781 ttgctgcacc tattgcactt gatatgtgat catatccaga tgcaatgtct gtaacaagcg
      841 gtccaagaac gtaatatgga acatcgccga ttagcgattt tgccagtctg acatttgctg
      901 caacttcgtt tagcggcaca tggccaggcc cctcaaccat cacctggacg tcgtgttcat
      961 gtgcttgctt gacaagcctt gagatgttta tcatctcttg gacttggagc tcatcatgcg
     1021 agtcaagaat tgagccgggc cttaatgcgt cacctaagct gaatgtgacg tcgtattttt
     1081 ttgctatttc tattaggtaa tcgtaatgag agatgtaggg gttttccttg tcgtatttga
     1141 gcatccacgc agcagtaatc gtgccgccct tgctcacaac gcccccatat ctttgaacct
     1201 tcaaaattcg ctttgccatc tcttttgaga tgccggagtg gattgtagta taatctactc
     1261 cgtccttggc gttgttttca aatgcattaa gatagtcatc ctctgttatg tcaagtggat
     1321 ttttgtgttt ttcaacccca tagttgtatg cttcgtaaat tggaaccgtc ccaaacgtga
     1381 ttggcgctgc ctcaagtaac gccctcctaa tcgagccaac gtcgcccccg tcgctaagat
     1441 ccattatggt gtcggcgtgg tgctttactg caattttggc tttttctatt tcctcgttaa
     1501 gatcgacttt gagtgtagat gtgccaatgt tgacattcac ttttgtttta agcccttttc
     1561 ctatccctac gcggtgcacc ttttgaggtc ttgaattgtt ggcaggaatt ataattgagc
     1621 cgcttgcgat tgcctttctt aggaattctt ctgtgacagc ttcgtctcgt gcaacctgtt
     1681 tcatctcgtc agtaatgacg ccccgtcgag cagcactaaa ctgtgttccc atgtatctag
     1741 tatgatgatt ctagatttaa acagtaggca agcggagctt ttagatcttg ttaaatactg
     1801 aacagttttt gaattacttt ctgctgattt acaaaaatac tatttacggg agaaacatca
     1861 caaaacataa tgaacatttt atcaataggt ggatcagacc cttcatcggg tgccggaata
     1921 cagtccgata tcagggccgc atcagcactt ggcgcaaatt gtttttccgt cattaccgca
     1981 ataacaagcc agaactccac tcggttttca agtgttgagg cagtctcagc taatgcagtt
     2041 gcgcaacaga tcgattcagt tctttctgat tttggaatcg atgttatcaa cataggaatg
     2101 gtgtatgact cgaagataat agaagaaatt tatttaaagt taaaggacca gaaggtacca
     2161 atcgtcgttg acccagtaat aaaatcaaca acgggcgggg ttttgctaaa aaaatcggca
     2221 ctttttgcct tcaagaggtt gatcattccg ctatcatacg ccgtaactcc caatgtaaaa
     2281 gaggcggaga ttctctcagg ggtaagaata gctgggtttg aggacctcat tagggcggca
     2341 aaaaaactct cgcaccttgg tgcaaaaaac atcgtaataa ctggccacgc ctttacaaaa
     2401 aacaagatca gtgattttgt atatgagaaa ggaaaacatc attccatttc tgggaaaaaa
     2461 cttggtggat gggcccacgg gggcggctgc aattttgcaa ttgccctatc atattcgatt
     2521 gcgcaaaaaa aatccgtcgc agaatctgtg agatttgcaa aagagtttgc gtaccagtcc
     2581 ataaaatccg cacaagactt aggccatgga atcaaaatca cacgtcccaa gccagacaag
     2641 ataagaacgg aacttgcttt tgcgatacat gaatttacaa aattggatgg cgtgacttgc
     2701 ctaataccag aagtgcagac aaactttgtg tttgcaaaac aaaacgcaaa atcaattgat
     2761 ggcatattag gagtaggtgg aagaatcgta aaggcaggaa aaaccgcgat agttgccgga
     2821 gagctagaat atggtggctc tcatcacgtg ggatccgcag tactaaccat tcaaaaaaaa
     2881 ttccctcacc taagatctgc agtcaacatc agatttgatg agagatttat caaaaaaatg
     2941 caaaaagcaa agtacacaat tctaagctac aacagggcag gggagccaaa aaagacaaga
     3001 ctcaaagaaa actcgactat atcatgggga attcaaagcg cgataaaaaa ttctgccata
     3061 ccaccagatg ccatatacca cagaggagat gtcggaaagg aaccgatgat cattgttttt
     3121 ggggaaagtc caagggatgt cctagccaag gtcaaaattc ttctcaggtt caaataattt
     3181 tgtaacgcaa cataaatacc caatattttc atctagtcct gtgaaagcag tcattcttgc
     3241 tggaggactg ggtacaaggc ttcagccata tactacgttt ttgccaaagc caatgcttcc
     3301 gctgggtgaa aaaccgctct tagagcacct aatagcatgg gtaaagcgca acaagataaa
     3361 agaaatcgtg ctttgtgtaa gctatcttaa aaaaacaatc gaggattatt ttgaggacgg
     3421 aaacaggttc ggagtcaaaa tagagtacgc cgttgccgac aggccccttg ccactgccgg
     3481 gcagctaaag actgccgagc cgtttgtgga cgataccttt gtctgtgttt atggcgattc
     3541 ggtctttagt ttcaatttag ctaagatgat aaaccagcac aagacaaaaa agtcctttat
     3601 tacgatgagc ctgcggcaat acaataccac gctaaagtac ggcgtcatag agactaccaa
     3661 gtcaggcaaa gttactgcct ggaatgaaaa acctccgatc agtgccaaca taaacatcgg
     3721 ctgctacgtc atggagccgg gcgtttttgg actaattcct gctggcaagc cgtacggaat
     3781 ggacgacgtc atcaaaaagg cgcttgcaaa gaaaaaagat gtcaacagct ttctaataaa
     3841 gaacggattt atcgacattg gggacaagat ctcatacaaa aaggcatatc aggaatttcg
     3901 agaaaagctt ggcaagatct aatgtccgaa ataacaattc gcaaaattca ggaatctgac
     3961 atagattgtg gctttttaga atcgctagac agtctgcgca aggcaagcga cctcgacaaa
     4021 aaaaaggcaa aagagattct aagaaaaata gctgcaaatc cgacccacgt gatttttgtt
     4081 gcactaaatg agaaccgagt cattggagct acaacccttc taattgagca gaagtttatc
     4141 catggtggtg gactagtcgg ccacatagaa gacgtcgtag tatcaaagga acaacaagga
     4201 ttaggcatcg gcgagaaaat catgctagct gtgctggaat atgccaaagg ccaaggctgc
     4261 tacaagacca ttttggattg cgatgacaat gtcaagccat tctatgagaa gattggcttt
     4321 aagagacatt caaacggcat gagatacgac cactaaaaat attcctttat tggccgcttt
     4381 tttgttctaa ataatagtcc gccaacaccc atcactgcaa atgacactgc catgaacagc
     4441 ggcggaatta tctctactgc atacacaaga tagtttctgt caatttgcgg aaccagtgtg
     4501 tttgcataaa gcattcctac aaaaagaact gcaccccctg caataattat agagccaact
     4561 tgttttgagc cgtatcgctt tgtcatcaaa aacgcagttc cagcaatgat cactgcaggt
     4621 gcggcaccaa ttgagatgaa catcaagatt tgcgccgccg gctctacttc tactctaggt
     4681 gctattaggt aattgtaaaa tacgttgatt tctccgcaaa acattgcaaa gagtccaagg
     4741 cttgcgactg caagatactt ttctattgcc acaccctagc aaaaatagcg tagataaata
     4801 aatcgtgtgt atcgctaaat attttttcag gtgattccga ctaggcctgc aagttccttt
     4861 ctgcatgcgg caccaagctg ctctgctgct ttttctggtg gaattgagtt gataaacaca
     4921 ccatagcccc tctccagtcc cgaccaagca gacgtcattg gataaatctc aatgtgccag
     4981 tggatctgcc tactgttttt cttttcaggc gagaggtgaa ataccaaatt gtatgagata
     5041 ttttttagcg agtttgagag gccacccagt gtcgcacgca aaatgagcga cagatcattt
     5101 atttcctttt gagttatttt agaaaagttt gtcgtgtgtt tttttggaca tatccagtac
     5161 tcaaatgggt acgatggtgc ccacgggcaa aacgctaaaa atccctccgt ctgaaggacc
     5221 tgccttggcc cgccagtctc cgtgtttacc gtctggcata gcgggcatga gcccttttcg
     5281 tttagaaacc ggtgcgaggc ctcggcttcc tgctcaatta atggcggaat tgtcgagaat
     5341 gtgaccatgt ttatgtgcgg gtgcggagtt atgcttcccg cctcctttcc atggttaatg
     5401 taaattgaaa catatgttac gccgcgctgc gtgtacagcc accgcatcct atcctgaatc
     5461 acaactagaa catttgacca ttgttcgact ggaatggtag caagcgagtc tttgtgtttt
     5521 tcagaggcca cgaccacata atgatacccg tatgctggtt cgctgtaaag gggcctgtcg
     5581 ctgtacgagt tttctgctga gatggtaact gccgggacct tgctttcaaa gactcggacg
     5641 ctccaatttc gaacataatt atcttcgctg tcctggagtc tttggagcat tccatccttt
     5701 gcgacaaggg agagcagcga tggatttgtc atggactcat ttccaggaca gagcgcgcat
     5761 ttttttgact cgctttcttt ttcgtcattt tgtgaaacta tgacaaacct ttctgaaagg
     5821 tagtcttttc tcatgctccc catattcgat actatgaaat cgtgtgagtt aaaacctttt
     5881 caacgatcgc gtcttagcta aaaatttttg agtatttctg taaagtttaa agtgatcttg
     5941 gtaaagcaac caatcgggga tcaatcatat cggataacac cagtgtgaga attgtctacg
     6001 tattattgga tggtgtaggt gaccttccac accctgatct aaagggagca actccacttg
     6061 attatgccaa gaccccaaac cttgatcagc ttgcaaaaaa tggcgcaatg ggcgaggtaa
     6121 tctcagttgg aaagggaatt gccccagaat cagacatcgc agtctttaac atgctcggat
     6181 acaagttcca gcattccgaa tatgccggaa ggggcgtaat tgaggcaatt gggactggaa
     6241 ttgattttag ggacggagac ttggcccttc gcggaaattt tgccaccata gatgatggtg
     6301 ggataataat tgacagacgt gcaggacgca aaatagaacg cgaggacgca atcgcagtcg
     6361 caaaagaaat tgagggccaa atcaagttct cagagcccgg agcgtcagta gttgtctcac
     6421 ccacaatagg gcaccgggta gtagtcagaa tacggtgcga tggaaaggca ttatcatcag
     6481 aaattacaaa cacggatcct gcctattcga gagtagacgg aatgggaatc gcaaaggcag
     6541 taggcgacta tatgaaaata gagaggtgcc tgccgctaaa agacatccca aatgcgtcgc
     6601 ttactgctaa tttggtcaac gaattcacag aacaatcact aaagataatg agggttagtc
     6661 agacaaacca aaaaagaaag cagcagggaa aaaaactcct aaacagcatt ttgctgcgcg
     6721 atgcaggaaa ccattatcca aaggcagtcc caatcaacga gctttattcg ctgaggtttt
     6781 catgtatagt ggatatgcca gtggaaatag gcattgcaga aattctaaag atgaagacgt
     6841 ttagcgcggg ggggctgaca gactatgaga aaaaggccca ggttgccgca aaggcaatgg
     6901 agactgaaaa cgcaatctat gtgcacctaa agggcccaga cgagtttggt cacgacggcg
     6961 acgcgttggg aaaaatgaaa aacatcgagg aaattgacag gagattcttt ggaaccctgc
     7021 tctataacat agatacctcc aaagtggctg ttgttgtttc cgcagatcac tctactccgt
     7081 gcatcaacaa agggcacagt gacgaccctg tcccggttct agtgtctgca gattctgtca
     7141 aaaaggacaa cagtgaaaga tttactgaaa agaatgcaaa ggtcggaagc atcggactct
     7201 tagaaggagc tcaggtgctc aaaacggcac tgcagatgat taaatagtga aaagcgagat
     7261 ctgagcggaa ttttttatct cctgtatttt ttggcgcagt gcgtcaatat ttattccatg
     7321 atacatccct gcggctcttg cgagcttggt ctgggcccta ccaagaaccg aaaggcagat
     7381 gtcgtcttcg gccttttgat agtgaaccat ggctgcggca gccagaatta ttccctggac
     7441 tagctccttt tctccctcgt agcatttttt ccaaacgccc tctaggactt cgtgggcctc
     7501 ccagaagcgt tcgtcattaa aatacgagat gcccattttg attgcctctt ctttttctat
     7561 gtgttcttct attacgtgtc ttgcgtgatc cagcggagca attggtgcca gtatttctga
     7621 gagggtatca atttttgatt tgtctaccga tacgtcaaac tcgacatatt ttgttgaggc
     7681 tcttgtatcg cgaattatca cgttcatgcc ggatgtaagc tgccttgcct ttaagagaag
     7741 ttttccagtg tcttgtggag tgtagccggt gttttttaga tgcagcatga acctgtccat
     7801 gatttgaaaa tggcgtgctt ttcttaaatt tcttcttgat ttgcaatcaa gatttatatg
     7861 aaatagcaaa aggatgcaca atacatgtcg atgattgaaa caataaacga tgtaaacaac
     7921 accttccttt caagaaggga gattacgtgt aatttcaaag gagttgccgg caagctcaaa
     7981 aagctagagg cagctgagat catatcaaaa cagttcaagc tcgatggcaa agtagtcatt
     8041 ccgattttta tgaaagcgca aagcggcagg ccggatgtga ctggaacatt ctatgtttat
     8101 gatgatgaag tgctggcaaa aaaacaggtc aacgaagtca ttttcaaaag acttgaaaag
     8161 gcaaagggag aagctgctgc agcaactgaa aaagctgctg aggcagaaaa agcagaagct
     8221 gaaaaagtag tagagacaga aaaagtagtt gaggcaagtg ctccagcaga gacagaaagc
     8281 aaagaagagg tcaaggagta atggctggaa aaaaggcagc tgccaaggca accgggattg
     8341 cagcgtacta taagatcagc ggtgacaagg cgacaaggac acataaaatc tgctcaagat
     8401 gcggcaaggg agtctttatg tcagagcaca aagacaggcg tacctgcggc aagtgcaacc
     8461 taacagaatt cattcagtag agtttttttc ttaaattcgt aatgttggtt acctgtttag
     8521 caattttgga atcagcatag tttgttcttg ttttgcgatt tgttcgattt ttttcttcaa
     8581 agcaagttcg tctgatttgg tctgttctga gacttgaatt tgtggatatt catcaaaagt
     8641 ctttccgtcc aactcacgcc caccactttt tggcctaatt ccaccccatt gtttgaaaaa
     8701 caccgcgaca tcttgttgct tacattgctt tacgatattt ttgatccagt ctttttttac
     8761 ctgacgaaaa ccatagccac tttctcctcc tatgataatc caatcaactc cctctaggtt
     8821 tatctgacca acatctccaa tcaatggctc aaagctaatg aatcttgcat ggcaacgtac
     8881 tctgcgtaat tcatcaattc tccagttgta atgttcactt tctatgcttg tgcccatcca
     8941 aatgtgatga ggtattttta ttttgaattc tgtctcaaat aggtttgaga atcgtgccat
     9001 cctttgtggt ctttttgtaa gtatctgata agtatgccaa ttagccataa tcattgaaaa
     9061 aaaacatttc tttacaaatt caaagtcaga tttctcatgg aaaaggtctg acatactgtt
     9121 aacaaaaatt ttctttgatt ttctccaagt taaaggcaaa tacaaatcgt cgttatgctg
     9181 cacaaactga aaaccgcgtt catatttttt cacgcccatc atttgtaacc ttttggttag
     9241 tctttctgca taacagtttg cacagcctgg tgagattttt gtgcaaccag tagttggatt
     9301 ccaagtagct tctgtccatt caattgcgga atctaaaccc attgacacga ttcattagtc
     9361 agtatccctt aaaggtttca ccgtaagttt agacttccgc tttagctagc tcttcgctag
     9421 taatttgctc aaatacttgt tccaacatag tcatatgata cattggtgat gataaaaaat
     9481 ggccagagac ccatatcttt gggcaaaaga aaaaattagt ttccttctag agaaaacaga
     9541 tataataaaa aacgatggtc ttaaaaaatc tcctggtaac atatggtcaa ttaaaaaatt
     9601 acttgccttg gattactaca taggggggtc gcatgtttta ttcaagaaaa attttacgaa
     9661 ttggcattat gtagatactc attgtgggtc tggtttaata ggatttgaaa aggaaaaact
     9721 acaattagaa cgatttcccg gatctccact catagctgca ttacgagcca ataatcaacc
     9781 atttacagat tatcattttt cagatattga tggtgaatcg ataaattctt tgagtcagag
     9841 gctacaaaaa atgaaacttc aggtaggcaa caaggactat tccccgatgg ttagaagttt
     9901 tgaggccacc gcagatcatg tgacaagata caaaaaatgg ggtaatgcct ttctgatttt
     9961 tattgatccg gtaggattta cagaacttaa atggtcggtg atgaaaaaac ttctagaaat
    10021 caaaactgca gacatatttc ttacttttat gacatcattt attgccttga acaggacaaa
    10081 tgctcatcaa ggaactgagt atgaaaaaac attcgatgaa gtatacggag ttccagattg
    10141 gcgtaaagaa atcggagtcg atgccttgtt agagctatat cttaatcaaa taagaaaatt
    10201 ccgaaattat gtatatacta ttccagttca cagggctggc gaaaacagat tgtatgacat
    10261 aatcgtagct acaaatagtc gcggtgccgg tagcatcatc gattatacaa agaaaatcat
    10321 ggaaatttta acaacggagc ttattgaagc tgccttaaaa gtgactacca aaaaagaccg
    10381 tgatcttgat gaatggtttg atggttaatg ttgctttact aactcatatc tcagtagagt
    10441 tttttcttcc gtagttttga ttcgcgttct tcgtattctt tgattttgtt tagcagaata
    10501 ggatccagac tcacctttgc aagcatctcc gctgagagct tgactgtgtt tgtgtcaagt
    10561 gagatgttgg ttaccggcag gtgttttgca atccagagct tttcggtctt gtcctcaagc
    10621 ttgtagtcct taaagcccgc aggaaacttt tttgtgatgt gcgttagaag cgttttgtca
    10681 tcaaataccg ctcttggctc aaagagtctt gtgctgtccg catacacatt ctcaaaaagg
    10741 tttgcagaga cctcatcaga tacaatctcc atcgtagata tttttctgat aagctcaata
    10801 tagtgcagaa actcgtgcgc caaaatcgcg tgaatagtcc cctttagacc aaatgcgaca
    10861 agcggcgcag atatttgtat gaccacttgg aatttttctt caaaaaatac cggaagagtc
    10921 cttgcaaaca aaattccgta ctggaacgac atggaatccg agcccgtcac tattgcagac
    10981 ggctctacat atgatattgg gtaaggggtg ccggaggcct tttctattct ttgtattcct
    11041 tctgtaacta gcggaaacct ttggcttatt gtatcaaaga tcttttgcgg tatgactcct
    11101 ttttggtgag cctctcgcac ctttactaga tgatccaaca aagtaacgat gctgcaaggt
    11161 gtaaaaagtg ttagtttgtg atctttactt gttcaaaatc aaatgtctcg cattttgatt
    11221 tgttctccaa aatgtctgat acatttggaa agacatcctc tccgctgaca agccgcttta
    11281 gtggaacgcc gccgtcaatt actagtgaga ggtaaaacga gttttccgag ttgattttgt
    11341 attttacgtc gtagatggat ttttcagccc tcttgccgga tttttcataa actgcaatcg
    11401 tatcctttag ctgtaaaagt tttgagagcg attctggttg tattggctgc tcagtagtaa
    11461 ttaggagccg agtttttgat acaaagcgca ccggcattgt cggtattttt tttatctgtt
    11521 ttagcgaaaa tacgattatc ttgccaagcg gtattttttt tggcaacgta aggttgcgct
    11581 tttttggccc aatcagcttt gcaaaaaaag gcctgcctga gcccagaaca aggcttgttg
    11641 aatcctcgcc cccaatccac gttattttga cttgggtggc accaaatttt tcaaaaaaat
    11701 actcactcat tgcgccttcg acgctgtcat attctgatat gccgtgataa ttacaggatg
    11761 agcacccctt gccaaggcag ttagagcagg gtttttgttt ttgtggcgtg ttgcggtcgg
    11821 acttggtata ccgcccagaa agaaagactg gctttgagtg cacgtcgcac gactcttcct
    11881 taaagttaat cgtaaaggta atgtctggat ctagctgctg gcccttcttt tttgtttttc
    11941 ttgcaaactg ttttgcaagc tggcgcgtaa cgtttgtctt cactccttcc atcccccgta
    12001 gcttaaattg cgatcttagg tggtcgtccc tgtccaaaat cgagggcttg agctttgcgc
    12061 caacgaggaa tgtttgaaaa ccatactccg atgatgcctc ctgcattttt gcaacgtaaa
    12121 ccggcacatt atcaaagatg tttttgcaga catagcactt tgttgttttt atttttagtt
    12181 tcttgtggat tttttctcca agctttttgt ttgatgagag gccgagcttt tttgcaaaca
    12241 gtcttccaag gcaataatcg cacaagtcat attcgtgtag tatttttttt gatgattcca
    12301 gtactgctac aagcggtttt tgtctcacac caaaaaattt ggcccacgac taattaattt
    12361 gcctagtcag gccctatatg atctaagact cgtattttac tggaaccata ttgatgtaaa
    12421 ctgttatgta ttggtcaaca ctcgatacat cagatccgtt taatacataa atgtcaaata
    12481 tagccattgc ctcttaaatc aataaggcaa aaccatacac caaattctga gcagacacgt
    12541 atgatggaaa tattccgtca gatggtcaat gaatgcatca gaatcggtct tgagaacaac
    12601 tgtaccacac tcaaaaaact atcttcgttt tcatactcca agctgcaaaa ctacgatatc
    12661 ttatcatact acaaactgac tgcaatatcc caagcctgtg gaagactgat ccaaagaaaa
    12721 aaagacatca aaaagggtaa aaagccaaaa atcccgtacg tagaaaagct gtttctggtt
    12781 tcatgttatg gctttaagat caatggaatg ttgttgtcat ttccagtcag aaacagggag
    12841 tttgcaaaca tcttgttaaa tgattatgtc gcaagaaata tttcagatac atccctcaaa
    12901 ataagatctt ttacaatgac tccagaatca ttatcgctat caatacaaaa agaagtagaa
    12961 ccaatcaaac cagaaaaaac tataggcata gaccgaaatc tcagaaacat tacctttggg
    13021 aatgatgaca aggtaattct agtcaacacc gcagaactgc tcaaaataaa ggaaaactat
    13081 acctacatca aatccacttt tagaagaaac gatcacagga taaaaaagaa aatcgtgggt
    13141 aagtttggaa tcagacaagc tagaagaata caacaacgaa ttcataagat ttcaaaatca
    13201 ttagtagaat attccaaaaa gcaaaaggca atgattgttt ttgaggatct aaaaggaatc
    13261 caaaaactct acagaaaggg aaatggtcaa ggaagaaaat tcagaagaaa actcaactcg
    13321 ctatctaact acgagcttga aagacagaca acatacaagg cagattgggt gggagtccca
    13381 gtttgccacg taaatccaag aggcaccagc aagctttgtc caagatgcgg agggagactc
    13441 caagaggaca ggcaaagacg tcgtgatttg tggtgcggta tttgtgagaa atggcaggac
    13501 agggatgtcg ttgccgcgat gaatatttct cgcaaggggt tggcaaggtt gaccaatcct
    13561 caaggcgata caaatgaagc gatgaggggg aacctgattt ctgattcaga tcaggaacca
    13621 gtaatcctca gagtcgatgt atcgaagttg atcacggagc tggtcttatg atcagatcta
    13681 gtagattaca aagaacccct agccgaggcc catcttgcgc aaaaatccct gcatctggcg
    13741 gccctttgat gccttcatca tgcttttgga gttcttgtag tttttgagca gttcctttac
    13801 ttcgtgttct ggccaccccg agcccctcgc tattcgcttt attcttgatg cgttgagcag
    13861 gccgtctggg tctgcctttt cctgcttgtt catgctctgt atgatgtagc gccagcgccc
    13921 tattctatcc tccatctgat ccagctgatc ctcttttacc attccagaaa gtcccggcat
    13981 gttatccaaa aatcccctaa gtgagccgac ctttgtaacc tcctctagct ggtagtaaaa
    14041 atcatccatg ttcatcttgc cactagagat tcgcttaagg cgtacctcgt ctgcttcgct
    14101 ttcaagcctt tttgccatat caagcagtgc ctggatgtct cccatgccaa gaagcctacc
    14161 aacaaacctt gtcggagaaa acaactccaa atcatcgatt ctctcgccgg ttcccacgta
    14221 catcaccttg gcaccagttg ccgcagaggc ggcaagtgcc ccaccccctt tggctgagct
    14281 gtcaagcttt gtaattatta ttccaccgat tggaacggtt ttgtgaaatg attctgcctg
    14341 ggaatagcac tgctgcccta ttgttccatc aattacaagt aagaccaggt ccggtgctgc
    14401 gaccttgctt atctccttca tttcgtcgag taattctttt tcttctttgt ggcgccctgc
    14461 agtatcaatt agtatgatat cgaggcccga gctctcatag tgttttagtc cgtttttgac
    14521 aatttgcgtg gcatccttgt tgttttcttc gccatatact tcggcgtgtg ctttttcgca
    14581 gtttgtcttt agctgcacta gtgcacccgg cctgtaggta tcagcaccaa tgactcccac
    14641 cttgtagccc tgtcctgtca gaaacttggc aagcttgcca gcaattgtag tcttgccgct
    14701 cccctgaatt ccaagcagca ataccttgtt tagcttgcct ggcttgaagg aaaactcgtt
    14761 ttcctttcca agtaggtttg ccagctcgtc atagagaatt tttacaatgt gatcctttcg
    14821 tgataggccc gggggcggct tttcgtttat tgagcggtac tctagatttt ttgtgatttc
    14881 taaaacaagc ttaacgttga cgtctgcctg caagagtgcg cgttgtacat ccttggccaa
    14941 ttccttgatt agctcctcat ccacacccga tgattttaca atttttttaa ttgccgcctg
    15001 caggctgttt ttgagattgt ctaacattgt ttttctattt ttgcctgcac tatttcaaac
    15061 cttcctctgt acccatccca ttcttttcta gagtcgtgga tttttattgc gccgcaaaac
    15121 agcgggcagc tgatcacaaa tgtttctgcc tcccctccct caaagctcaa attgaatttg
    15181 tgtttttctg agagtagtat caattcatca agatcttttt ttgtgatttt ttttccaagc
    15241 catgaatcat ctaagccatc acaggaaact gcagttatta taaattcgaa tccggagtct
    15301 agcagctcct gcatgtattg tttttgatcc gttttccaaa gcggcgatag tatttgcagg
    15361 cccaggcttt ttgcaatatt ctcaaactgt gttttttgaa actcgcttag tatgccaccg
    15421 tgaactattc cttctatgtt gtagtcgttt tttgcctttt ctagtgccgc ttttagcagc
    15481 tcgacttctt ttttcgtatc gacagaatcg gcccgaacca aaatctgcgg gatgttcatt
    15541 gattttgcct gcagaatcga gagctcgatg tttgggtggt ggagcaggtg gctttcctcg
    15601 gagctagtca gtattgtgac aaggcaactt acggtgtggc caagcttctg ggccttgtaa
    15661 attgaaaacg tgctgtcctt tcccccagaa aacaaagctg caagattcat tggttttgat
    15721 tatttgcagt caggtattaa gtaagcacag gatgcttcaa tactcataat cgtcatcata
    15781 gctcagatgg cttttccaca catcatcagc atccgaactt aggatttaat tctcattatt
    15841 ttagttctgt ccattacctt ccagtattca accatttggc cctgttccag tttccctttg
    15901 acctcgtcgt ctatcatctg ggcatctatt gtctcaaatg tctcgctgtc cattacttgg
    15961 acctgatcac cggtaatcga gatgatttgg ccgctccttt tgtcaattag aggaacgtgc
    16021 acctgcatgc tgactgggcc gacgtgaggc cttttttggt tatcaaagac tccgactcct
    16081 actattcttg cctttgcagc tccgtgtttt ccgggcttgc tggtgtcgta ttctacgatt
    16141 ctgcacggct cgccggttgc ttggtcgccg actggcaaaa gaatgtatga gccgatcttt
    16201 agtgaaccaa ggtctgaagg cttgctcata accagagcaa tttatgctca gtatttaata
    16261 tttttgaaga gagaagctga tcagcggatc actttagttt ttcaagaatt gagaggccga
    16321 ccaaattatt catcgttatt ccttttctgc aaacgtttct tctggtttgc tcgcagtact
    16381 tgtttacgct ttggtggctt ttttggctcg atacttccaa gacgacaatg ttgttgctgt
    16441 acggaaatgt gaacttgggc ggatcagctg caacggtgag catgtttcca gtcgatgcct
    16501 tgcgaacatg atcgtttttt gacacaacca tcgcaagatc agacaaatcc tcgtctgatt
    16561 ttgaatattc caggtaatac acggacacaa agttcattgt gccgtccact tgcctttcaa
    16621 atatctcgct gtttgtgtta aagacaaagg aggtcttgct ttgctggtat ctttggatgt
    16681 cctttacgat gttgttgttt gcatacttga ctaggagata gtggtttgcg tgcgacggaa
    16741 agtacggctt gctgtcctgc gcaaaggtgg ccgtgacaaa atacatgttt tcaatatttt
    16801 tagagtcctg ccaagaatcc tttatctcaa ttgatttgtg gtcgtgtgtg tacggcacac
    16861 acgcgtatcc atctagtgaa gtttccgaca ctaaaagctc tgattttttt agaatattta
    16921 acagttcttt gatcgcttcc aaaaatgaaa aagtgtgttt gatccccgaa gcgcatctat
    16981 ttaatacaag tccgcgccga ggttttttgt cccaagctag ctcagcctgg tagagcttcc
    17041 ggctgtagtg gttggctttt ggctaaccga aatcagccta ttaggcgcac agaaaccgga
    17101 gcgtcgaagg ttcaactcct tcgcttggga ctacaaattc ttctcttttt atcaaatccc
    17161 ctatagatcg gagattcaca ctttattgca aaggtgtaag atgcattatt ttcaattata
    17221 ttatttttct gaacacatta ca
//
LOCUS       DASWKH010000083        27016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2080948, whole genome shotgun sequence.
ACCESSION   DASWKH010000083 DASWKH010000000
VERSION     DASWKH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 27016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27016
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080948"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VGA92_06445"
     CDS             <1..387
                     /locus_tag="VGA92_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HEX9846088.1"
                     /translation="YAGPKKEIGFYHDIRIDHDSKSKLFEGITDPFTVFHWHGDTFDI
                     PKNATRLAHSEMYNQAFRYGSAVGVQFHFEVDRQIILSWLDNAQDLNLSYIDPQKIKS
                     QIDSNIKDVQENMRIFYKNFKSEFNL"
     gene            complement(446..871)
                     /locus_tag="VGA92_06450"
     CDS             complement(446..871)
                     /locus_tag="VGA92_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846089.1"
                     /translation="MKKRAISPAIATIFLIGVAVVGAIAAGNGMFKQNEITQKTTKLD
                     LIDVNLVKIASDKTYFAATVKNTGMTTFSSVNVSFVDEFGNFYTITSLTPLEPGEQFG
                     DHLIENVAVNLGKKYLINIDGTTPSGSTFRSADTINVRG"
     gene            complement(894..1172)
                     /locus_tag="VGA92_06455"
     CDS             complement(894..1172)
                     /locus_tag="VGA92_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846090.1"
                     /translation="MELQNRYYQNLEDVAKILEIAKDSLVLQIKKISRICNGILETDS
                     HILQIRGLKPTQHGLLYLIHISDKNSKKCILNTPVLLQSPRRSRFFVA"
     gene            complement(1196..3055)
                     /locus_tag="VGA92_06460"
     CDS             complement(1196..3055)
                     /locus_tag="VGA92_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9846091.1"
                     /translation="MIADLKRRQAFYDVRFLKLENLQQTKTKFVYEIKPTNFGMLPDD
                     KKMVALSKFFNILASIQRPIRIIMLKDPLEFEMGDEVRSLQVPRTYLVSVEPLEQILQ
                     QIGVDYSMATTEPSWKIRSEMLSHLVLEDGSFAKCYTLYRIPASLSAAWVHNLLPHVE
                     MVSIRMKPIENHRAVSIINRYIGLVGASITKNPDSYYKYEKGTGLLAALTRQETRMFT
                     CSLAVMISAKDALALRIADKNFKTVIRSMLCSFEATITMQRQMLEDGIGKHLYFELGS
                     SAIFYPFVSADMIEVPNGVALGVNLNTSAPIIYDYTQRDNYNILVLATSGAGKSVTAK
                     TILVRLIKKYPTAHVFIVDPNGEYESVAKFLSINVVKVTEETNLGLDPFKLFKEEDAA
                     EIIGDISKGDDVVRKEFRAKAQGCKSIKDLYVRVDEDAKSYLKDLIAGSIYEILAGEP
                     RLEDKTVISLRGTSGEERVSMLLLLTLGKIWKKINSLPTSVPKILLIDEGWMLFNMAS
                     AGKFLDMMARVGRKFNVIFLFITQRPEDVIENQYGRAIAENAATKIFLQNTEQASEKI
                     KHAMDLSEQEVDMLKTFSRGQCLFLTKDYRLKAQITPSAEELKVFSTTPMDTP"
     gene            3064..4548
                     /locus_tag="VGA92_06465"
     CDS             3064..4548
                     /locus_tag="VGA92_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HEX9846092.1"
                     /translation="MKGRLVSFALISHRADLSKIQIIDTYKVNDASVYITNDGKYLIV
                     EPLLTKESHDIYQEMMEQLFYSLEPLSKTDDPINYIEKHMWKEAEDRAITDSFSKYFH
                     SLRYYLIRDILGYGILDVLMRDENIEEITAERFDTKIGIIHRKFSEFNILDTNISFGS
                     ADSMGSYIQRLMQKTGNTVTAATPIMNAMTKEGDRITVTYGQEVSLPGPTLDIRKFTR
                     DPFTITHLLEFGALTGLMASYFWMLFDAKAFGLVVGETGSGKTTMINGLMGLSNPRWK
                     IVTIEETPELQIPHYRWERLITRTSPMITQSNFDITIMELIKASLRLRPDFEIVGEVR
                     GEEAQFLFQSAATGHGGLTSFHGSSAESALNRLASDPINIKSSQQMLLWFIAHMTKLK
                     TADKKIVRKIVSVKEVMPKTESVSLHELFKYDQKTSSYNIDTIDQLIEKSTKVQDAAN
                     ILNVEPKEDLQKRIDLLGECQKSSAHSVEQVFSVISKYYANNLG"
     gene            complement(4647..4862)
                     /locus_tag="VGA92_06470"
     CDS             complement(4647..4862)
                     /locus_tag="VGA92_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846093.1"
                     /translation="MEKTSIIDSEFVTSKFSIRTTVGNVLNIIFRKKPATIPLDLATD
                     HHLIEASEKASESTKSRLQLHDDDKIP"
     gene            complement(4894..5295)
                     /locus_tag="VGA92_06475"
     CDS             complement(4894..5295)
                     /locus_tag="VGA92_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846094.1"
                     /translation="MKWGFKYPCYENIECDAIIQNWVNISYLGRRIKAEKTNANYIWI
                     KPFYELKATIIGRYSIIRTVTIVTPDLEILWSRSERIEFREIKPLESHLVISKNRIAG
                     KDSLVDGLTTNIPKGTSIKMEFYYSGFDQLK"
     gene            complement(5324..7039)
                     /locus_tag="VGA92_06480"
     CDS             complement(5324..7039)
                     /locus_tag="VGA92_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846095.1"
                     /translation="MKFSSSKILKNDSTRLSLSTNNKILEMVKPILKNKTITSIQGKI
                     NSEIEQEVRVSGKAYNPRVLVQQSIAYMIFSLFVLVPISVVLGLFVNPYLFFALLVCP
                     IWLYYPKLKLWFIVSERKSSIDDEMAFFTLYAGVMQSIGQSLYNSIIDVLGKGTFLTI
                     ENEGKMLSRNVQVFGMDQITALNEHGCSHPNFYFKNFLLGYVSISKSGGNLALYMERK
                     GEEFFHKAQFKYANYKSQAHIIGETMLILLTILPTMILISSFMLAEDSVNVVMSLSFV
                     LIPVVTIFIILMISLSQPRARNVIDFDFKAVFVAIFASVVSLLLGQQPWFVIGVGVAA
                     GAIFNFVVCTKQFREVSFVESALPDFFRDITEYRKIGIPIPNAIIKISEGRNYNKYFD
                     GLLSVISARLKHGVNLSDIFDSMLIRSWIAKTSFFVLGKIADSGGGTAQILEQITNFS
                     TNINQTKKETHASISVISYFAFLSPIMISYTTKEMSSILEKLNTGLDQMVQSAFNIEA
                     MLVSNDLIGFINLLNVISAISLGLVMSKLVHFSMKYTITVGIATLVSVLSIILSPLFP
                     TLVQI"
     gene            7349..7843
                     /locus_tag="VGA92_06485"
     CDS             7349..7843
                     /locus_tag="VGA92_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9846096.1"
                     /translation="MMATSKLGKNRRGISPAITSIILIGVAVAAGIGGYTVYASTANT
                     ASLKGAVTVEAVSLVKQSNGEEYLSITIKNSGNKAFASSSVNLQIDTDSASTGIQPFS
                     ASPLPAALNPGQTASVTARLINSAGTAITLQNIGETTPLEIVVVTTDGSTISELISVT
                     MSLS"
     gene            7883..8539
                     /locus_tag="VGA92_06490"
     CDS             7883..8539
                     /locus_tag="VGA92_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846097.1"
                     /translation="MKPIDLLYFEDVSPFHEAVFTFPIIGKLSMRQMSIVGFSAIISW
                     SIYQGSGNLLSMIPLCAGGYVGLKKFNVRPIELQIISVIKFMLFGKKAKQKRKFTKKT
                     AFKFPRQESKKLGLSEVFQPKLEIIGKEIRVREIFTDPLKPIRLQVKLETADRRPIVN
                     TQTRVEFDGNVISTLATNNNGEIEVLVIPQTLGEKNLLIYAKGFEQPVFEEILSIKSL
                     "
     gene            8705..8992
                     /locus_tag="VGA92_06495"
     CDS             8705..8992
                     /locus_tag="VGA92_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846098.1"
                     /translation="MLNQAALDHEDKQKYISIIVAAGIAGAIIFLAQDVMEWMIGVDF
                     ESPATGNIPPAIITMLGKILPFLRFIGGGLIVVAVIIAVSKLSLASRPVRW"
     gene            8994..9395
                     /locus_tag="VGA92_06500"
     CDS             8994..9395
                     /locus_tag="VGA92_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846099.1"
                     /translation="MSVLADLKTAIPLAGSLALYFYFKPSLSSETLFFFFGIWISCFA
                     LDAKITVSNPNLINHEKNIIFPILYRKFGPRLSPIIQLIIEVLAMALVVSLFERNLNL
                     ISMSVVSLVFGLAHLDAYLSNIKTIRNVLKD"
     gene            9494..10804
                     /locus_tag="VGA92_06505"
     CDS             9494..10804
                     /locus_tag="VGA92_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846100.1"
                     /translation="MKFKRRIRFQNHRGVLDAGIIILGMIVAIIAIVAATLAWSDVLE
                     VSFIKLMTEMPRFGYDVQSGHEIGNQNFEIYQILRNIAFIIMLFVLMFAGLSFMFEHI
                     NLVPPETGFKIISKSLLYVFFFFFFPPLWDLVAVTVEQTSLWILNPEDQTMPTKNVEF
                     LLTKLGSIESPEFTLDAIVAGISDPFGTLKNMFLSVFLATFKAIALLIFMFTAFLLGT
                     IRIVLTAIVTIAIPVTLMLSLIPFFKRVTNRFTDALLGLMIAPIFSALVITAGVAHLK
                     TLESTAQDPAVEWFAALAVMALATFIPAMIVPLLGSVMGSVSSITGRAVSTGAIMTNM
                     VASGVARTVGGVITGMTDPVRMETSTNPLNLAKLVFSGSRFLPMAKTNVEKIGGEISH
                     AGTMHGPLSGIHRMDENLSRESAQDFTLSRNVDKKSKSDSANEI"
     gene            complement(10830..11186)
                     /locus_tag="VGA92_06510"
     CDS             complement(10830..11186)
                     /locus_tag="VGA92_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846101.1"
                     /translation="MINFRLGEKIARAFEVKQSLHGKGIFYITNFGAYLETQRNGLVL
                     DLPFEILKAYKNTGRNSFKIEWAQDDHRLHYEFKVEGSAKQVFDTYTVANKEFAYSVS
                     EVDALKQKCVCQNMRN"
     gene            complement(11191..11910)
                     /locus_tag="VGA92_06515"
     CDS             complement(11191..11910)
                     /locus_tag="VGA92_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846102.1"
                     /translation="MKSLLAGEQIKEIFAINCKFGRSTFYISNFGILVENSNGVILEL
                     DHNSILSLQPIDKKSIKIIWKESNSAYDFVFNYEKPAEITTRYKSIHEDYLNAVRTIG
                     LKIEQQAREKITIQNPVIEPRFEKTPSYVLSEQIWNDCWFDKERNLYITHNKFFKEVN
                     DLQSRPHQIEYRIRTKDDGIVAKPEGVVLKFGIPAVQIPYKGQKTWFLLPTMSQKFLT
                     TKIEAARFDINSEQRIDYFSS"
     gene            11997..14447
                     /locus_tag="VGA92_06520"
     CDS             11997..14447
                     /locus_tag="VGA92_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846103.1"
                     /translation="MSSPYVIAAISLSSILALAAIIATSNGVMTNALLAQESIQIQTQ
                     KNRENLEIAISNDVLTMKNNGLDSITIKEIRIYSDADTTSLLARIKFPESGLQLKPLQ
                     KSSHNPSEFSVASFLTTTILGITDLGNVFVATNLLAENSGNGTGNGEAMINGMGLNSR
                     IVTYDYSGKLTHGYGDSGTLSSLKTYNQFSTMHDFAAQLLSTDSKITVAIPQFNTEYE
                     YVPQTQSVQATGTTNPNTLGYSQSRTVGGSAITAVGTDGITISGTGIVILKLNNYGDQ
                     TLVLEGNVPSGGLLQVGTDLQYDYINIPYDNAYGFRLYSGGLPGASYSQSPCSYANGV
                     GYNCCASWTYTQSFASPLILTTSSNYYSYYYSASYPFSILDGTQNPTTYSLNSIRMTA
                     GACSGWHSPYGSWPTTSPVPSGQTLFVFDKAYVMNTKIDLTSTFQMPHSFQAGKQYYI
                     FAKPNGGTITIKGSSFNPSMTPYLKITDLPPNIPYEIAKDGLLAVSGMADSIGTVSLT
                     LDDVKIGGTNPNGNLYLYPDSLKYRGSFSTVVFDNVNDQTIHIDTPEDRVYVTHAYVQ
                     IPVVGDVEVTDTYLDNHLSLSYLNGNYTTGESIKVPVISGYHDINMKINNVQTMTVIA
                     NVLGGTGLKVIQPSASTITQQNDNNLVSSITATTGSVSYIISTSSGIVTTSITATISG
                     DSEIKNHAYFGAPPPSPPSPPPRDPLKAWVDVYKNGVLVNQQQIYFNALPSSQNSGGV
                     SGSSSYVIDKYMYPQTVVNGVITTNVLPGDFVELYLYANIQADGSTPPIPSGHVFYHH
                     SGEGHATTTIHSGSILSS"
     gene            complement(14450..14629)
                     /locus_tag="VGA92_06525"
     CDS             complement(14450..14629)
                     /locus_tag="VGA92_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846104.1"
                     /translation="MSEPDYHKLTVEGDKKTQWICGCFETIDGYFWFCPEHKEALNRT
                     IEAQIDELDMTRVIK"
     gene            complement(14692..14949)
                     /locus_tag="VGA92_06530"
     CDS             complement(14692..14949)
                     /locus_tag="VGA92_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HEX9846105.1"
                     /translation="MEIHVYDTYVEAKDGHTMHFDVITSEKDHTKAIEYAKKWLGSIG
                     EADARVTTEECQFCHSQGAPKPVEDAIKTNGFFIQKMEGCP"
     gene            14991..15374
                     /locus_tag="VGA92_06535"
     CDS             14991..15374
                     /locus_tag="VGA92_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HEX9846106.1"
                     /translation="MHSLVFEIPKIDNITLELDDSGTSGLILLRLPFTVKINIWGEEI
                     YTEPIPVRAEEENAKPVVELFDVAYWPPGQALCLFFGPTPISKDEIRPYSPVNVIGKI
                     KNPDKKILSKLRDGMKVTIRLAGLG"
     gene            complement(15554..15751)
                     /locus_tag="VGA92_06540"
     CDS             complement(15554..15751)
                     /locus_tag="VGA92_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846107.1"
                     /translation="MKKAFDKTLGAFADLMDAVELLKTDITISNPREIEDLQNIISLL
                     DKASDLVIDAVIVFGKYENLR"
     gene            complement(16059..16256)
                     /locus_tag="VGA92_06545"
     CDS             complement(16059..16256)
                     /locus_tag="VGA92_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846108.1"
                     /translation="MKKALEITLGVIADLVDAIAIIKAEMRVSSSAETEDMQKIVFLL
                     DKAADFVIDAVVIFGKHETRR"
     gene            complement(16368..16895)
                     /locus_tag="VGA92_06550"
     CDS             complement(16368..16895)
                     /locus_tag="VGA92_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9846109.1"
                     /translation="MDTNVFISALKSDDPYHAEAGRVASRLRKGEIQAETSTLTLLEV
                     ASVASRLYHRAEGSKDDHKRKTFVVKTLKTLAGLGIRFVHMSGDTPFAVGNIKTDMPN
                     VFNESILLSIQTTLRTLDLMHVAAARHAKQNNRELGAFVTGDTELLRMKAQLSRITGM
                     PFLSPREYVIDMGLK"
     gene            complement(16912..17187)
                     /locus_tag="VGA92_06555"
     CDS             complement(16912..17187)
                     /locus_tag="VGA92_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX9846110.1"
                     /translation="MEILNVKVEKDTIEKLERLIKKKAYKNKSEAVRKMLEEHFEEHP
                     ELFGHDELQGILREADYMSDQQFEKLAARVFKGAKTAAQLVAQGRDR"
     gene            17466..18473
                     /locus_tag="VGA92_06560"
     CDS             17466..18473
                     /locus_tag="VGA92_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9846111.1"
                     /translation="MSTESDLAQRVEKHFAKEGWSVRREVKIRGRIVDIVAIKDGQIA
                     AVEVKGAGGDVEKGIMQSLHQKGAVNLSYLAIPQDTLTDSIKSTCRNLGIGLILVDKK
                     IKEAIRPKHTSSLISVKNKILGKESQKRSKVIESKSPLEIIFRTKSQILALKLLFLNT
                     SKEFHLNEIARRTGLTPPAVSKELAKLVRAGLVKRRNQGNLSFYKINEAGIIFGEMKR
                     IFLKYEIFSEILSRELAKEKIKYALIYGSFAKGTEKEGSDIDLLVVGDVSEDYLLRVI
                     PRIERNTGREINFILWTEKEFGDKVQKKIPLIREILETPIIMIVGDEDGFKRALKERI
                     G"
     gene            18511..18864
                     /locus_tag="VGA92_06565"
     CDS             18511..18864
                     /locus_tag="VGA92_06565"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX9846112.1"
                     /translation="MENAEKSLKTAENILKIEEWGAAHSSAYTAMLHTGRALMFSKGY
                     RPRAQDHHVAVVEFSKIYEKKYTAEVLAAFDKGRRRRHEFQYDDVDAISPSQARNIVE
                     SANAFVSKTKEILKM"
     gene            18999..19208
                     /locus_tag="VGA92_06570"
     CDS             18999..19208
                     /locus_tag="VGA92_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846113.1"
                     /translation="MRERLLWLCTCTGIEVMTATGVTAYLLYLGQATTAIISAIIFAK
                     LIAFHFAIEVHERLKRKRQATIIKS"
     gene            19236..19982
                     /locus_tag="VGA92_06575"
     CDS             19236..19982
                     /locus_tag="VGA92_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9846114.1"
                     /translation="MSSSGNQIDVIKKLYGEKNWQEIISFCKKMLEEDPKDLVALQNM
                     ATALVNVGKFDEVISCCDTVLQMNGTDEYALKNKIFALEKLRRYDDVISCCDLLLKKN
                     QNDTWSLDSKGLALNELNRHDEAISNYDKSLQIDPDNTVALMNKAVTLSFLQRYGEAI
                     QFFDRAQKIDKSIKEAALAKSDAYKKIGRENEAFLAAQGLLVEEIEGLVLEARQKKTK
                     VFDLYCLREFEGLEEREKKHNEKQDSKSKS"
     gene            20059..20808
                     /locus_tag="VGA92_06580"
     CDS             20059..20808
                     /locus_tag="VGA92_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846115.1"
                     /translation="MLRANALLFVGLALILFGAFNLTTDALAQDSDVEQKSGDQNDIV
                     DTILINLISQIGPIVAGFVSIGIQFARKQGLKISAEAEEYFVKSASSFVVNQGRFLYK
                     TIRDNPEYLDDFKKGVVPAKLKEVTRDKVIEQLQTELRSDEFTKAAKSMLEENLVTLV
                     ERSFTEHKTHMAEKTQSMLKDLVPIAVNATLLGFKSPDEVTKQKEKIIEDVLEAVRKN
                     FDLEDMLMPQDIATTHVKSELNKIVGSLKTP"
     gene            complement(20821..21030)
                     /locus_tag="VGA92_06585"
     CDS             complement(20821..21030)
                     /locus_tag="VGA92_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX9846116.1"
                     /translation="MLYHVHVEFNKDFVQVDGNQITIGVRSRPVGGAANKEIIKKLAK
                     HFGVSSASVVIKSGHKSKEKIIEIS"
     gene            complement(21045..21809)
                     /gene="sufC"
                     /locus_tag="VGA92_06590"
     CDS             complement(21045..21809)
                     /gene="sufC"
                     /locus_tag="VGA92_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX9846117.1"
                     /translation="MAVLEIIDLHVKRDGKPILKGVNLKTGPGEVHAIMGPNGSGKST
                     LAYTLLGHPKYEVTQGKILFDGEDITHASTDARAKKGLFLGFQYPTEVSGVGYSHFLR
                     TSYNALSKSLQSSNREVFITVREFQNYLKKNLAAVGLRDDFLGRYLNEGFSGGEKKRS
                     EVLQMAVLKPKVSILDEPDSGLDIDAVQAVAKAISEVSSADATTIVITHYARILKFLK
                     KLDYVHVFSDGRIIKSGDASLADKLEQEGYDWIVNA"
     gene            complement(21888..22847)
                     /locus_tag="VGA92_06595"
     CDS             complement(21888..22847)
                     /locus_tag="VGA92_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX9846118.1"
                     /translation="MNVTELETCCPECKSSLIDDVQSGERICSMCGIVVMEQLADYGP
                     ESKSSSLEEKMKNTRASGQTTYSQHDLGIATEISISTKDYSGKTINYQVANQMNSLRK
                     WQQRVRISTPRERRLANVLSVIGGACQNLSLSANVLETASMIYRNLDSYMEVKGKSVA
                     CIASATIYMACKQCDVVRSLEEVCSGAGSNKDIKMRTKLAAKYYRMLVMELGSVTAPV
                     ISMDKYISKISNLTNTDVRVERLALEVAEKTSDTNVADGKAPNGIAAAYLYIASILLG
                     QNVLQRDVSSVAGVTEVTIRNRCKEILTCYKMRLTLRPSFAKN"
     gene            complement(22844..23104)
                     /locus_tag="VGA92_06600"
     CDS             complement(22844..23104)
                     /locus_tag="VGA92_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gar1/Naf1 family protein"
                     /protein_id="HEX9846119.1"
                     /translation="MQEIGEIIHLAGSGRVIIRLKQILDEGNFVCDNTGRRIAKVTEL
                     IGPVASPYASAISLTNNIKKYTGKKVYFLDEPVIKKTRKKKR"
     gene            complement(23179..23481)
                     /locus_tag="VGA92_06605"
     CDS             complement(23179..23481)
                     /locus_tag="VGA92_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA];
                     GO_function: GO:0008312 - 7S RNA binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HEX9846120.1"
                     /translation="MKDYEHVVIWLDYFNKTLTKKMGRRLPKEKCVFDPSLKELVDAS
                     VEAGFSPKESEDKVRFPRRAYVRSGYVVLPKNFGAKTKILYKISEKLVARRAKSSK"
     gene            complement(23486..23866)
                     /locus_tag="VGA92_06610"
     CDS             complement(23486..23866)
                     /locus_tag="VGA92_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HEX9846121.1"
                     /translation="MKRSTENLWTSKITGGRRHPLRTKRKYEIDRFPNEAITGQQVTV
                     SRAVRGGDIKTGLKTIDHVNLALAEGKVKKSKILKVLENATNNDYQRRGVISMGAVLE
                     TEDGKCRVVSRPGQHGVVNAILIK"
     gene            complement(23914..24489)
                     /locus_tag="VGA92_06615"
     CDS             complement(23914..24489)
                     /locus_tag="VGA92_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9846122.1"
                     /translation="MVKVEDDTEAIARLEAALYSAGRPLSIEELIRASGTESRTKTLA
                     LLAGIEKRTKSAYKALEIDTLPDGSYVFQLRPEFNDIIRRYASKPILSKATLKTLSYI
                     AYMQPISSKQLVETRGTAVYSHLKELRQLDFISHQNVGRLKIYMTTSKFQKYFGISGD
                     SDILKQILFKKMRKNAAPQQVVQEAQIPQQN"
     gene            complement(24491..25159)
                     /locus_tag="VGA92_06620"
     CDS             complement(24491..25159)
                     /locus_tag="VGA92_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein ScpA"
                     /protein_id="HEX9846123.1"
                     /translation="MSEPAENISQAPVNVLFNPNTIAKKDVWEINIVQILEILIQILK
                     KADKKDLRVAGMAALSSSLIHRMKVERIFALQKAAMEKKPLRSRTDVDIQLLNIPYRH
                     EATYPVTLDELMDLLENLIGTIANPRSRRGGQLKFEPVEVPDFKDYFTSLENIIEKYE
                     ELIIRKIEVQGFGLLKDIIADLDTTDSIRCFFAVLFLAKDQKVDLEQQEDDIKITLIR
                     EDLT"
     gene            25258..26307
                     /locus_tag="VGA92_06625"
     CDS             25258..26307
                     /locus_tag="VGA92_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX9846124.1"
                     /translation="MKAARIVEPQKPLQVLEIETPKPKDVEVLVKVKAVGVCHSDLHL
                     WEGGYDTGDGFMKVTDRGVKFPVTPGHEIVGTITEVGGAVQGFGVGDDVLVYPWIGCG
                     ICVACRIGNDNLCDAPRSLGVFQDGGYAEYVLVPHFKFLAKVSGIELESAASLACSGL
                     TAYTAIKKSGANSQHNMVIFGAGGLGLMGVQIARAITNANIICVDIDEAKLAAAKEFG
                     ADHAVNSKDPEAVQKIMSICNGKGAECVIDFVNAPPTVKMGLAVIRKRGNMILVGLFG
                     GSIEISLVTIPLKAITIQGAYTGNYNDMLELLGLAKRGVINPIISKRYSLADANNALE
                     DLKARKILGRAVINP"
     gene            26412..>27016
                     /locus_tag="VGA92_06630"
     CDS             26412..>27016
                     /locus_tag="VGA92_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX9846125.1"
                     /translation="MGQWLSWVKSNEKEILTILDNLAIKAKETGEQLVLLFSDMNKAS
                     EYTTNIKKLERQADDLTRSIFAELNKTFITPLDREDIQRIASKTDDIIDFIEGIAGRI
                     KSYEVTLTPPYMLEISNELLNAIKEVEILISRLKGLKADKSLVEHCRKISDIEHNIDE
                     LYRAAVGELFKTNDAVNIIKLKDIYEAIEQASDRCLDVADVV"
BASE COUNT         7762 a         5701 c         5642 g         7911 t
ORIGIN      
        1 tatgccgggc caaaaaagga gattggcttt taccatgata tcagaattga tcatgactct
       61 aaatcaaagc tctttgaagg gattacagac ccctttactg tctttcactg gcacggcgac
      121 acatttgata ttccaaaaaa cgcaacacgt cttgctcatt ctgagatgta caaccaggcg
      181 ttccggtacg gcagcgcagt aggggtacag tttcactttg aggtggacag gcaaatcata
      241 ttgtcatggc ttgataatgc gcaagacctg aacctttctt acatagatcc tcaaaaaatt
      301 aaaagccaga ttgattctaa catcaaagac gtccaggaaa acatgaggat tttttacaag
      361 aacttcaagt cggagtttaa tctctgatct tatttcagcg tgtctataac taatcattgt
      421 caaaaacaaa ctatgagtct tttcattaac ctctgacatt aatggtatct gccgaacgaa
      481 aagtagaacc actcggagtt gtaccatcaa tattgataag atattttttg cccaaattta
      541 ctgcaacgtt ttcaattaaa tgatccccaa attgctcgcc tggttccaaa ggagtcaggc
      601 tggtaattgt gtaaaaattc ccaaactcgt caacaaagct cacattaact gaggaaaatg
      661 tggtcattcc agtgtttttt acagttgccg caaaataggt cttatcagaa gcaattttta
      721 ccaaattcac atcgatgagg tctagcttgg tagttttctg agtgatctcg ttttgcttga
      781 acattccgtt tcctgcagca attgcgccaa ctactgcaac gccaattaga aagatggtag
      841 caatagcagg actgattgct ctttttttca taataccaat ggacttacct gctttaagcc
      901 acaaaaaagc gggagcgtct tgggctttgg agcaaaacag gtgtgtttaa tatgcatttt
      961 tttgagttct tgtctgagat gtgaattagg taaagcaatc cgtgttgtgt cggcttgaga
     1021 cccctgattt ggagtatgtg agaatctgtc tccaagattc cattacatat cctggatatc
     1081 tttttgatct gcaaaacaag tgagtctttg gcaatttcta atattttggc cacatcttca
     1141 agattctgat agtatctatt ttgtagttcc acaaaacaaa aacagcagga attgtttaag
     1201 gagtatccat tggcgttgtt gaaaacactt tgagctcctc agctgacggg gtgatctgag
     1261 cctttaaccg ataatccttt gtaagaaaaa ggcactgccc tcttgagaac gtctttagca
     1321 tatctacttc ctgctctgat aagtccattg catgttttat tttctctgat gcctgttcag
     1381 tgttttgcaa aaatatcttt gttgcagcgt tttcagcaat tgccctgccg tactgatttt
     1441 ctatcacatc ctcaggtctc tgtgtgatga acaaaaaaat cacattgaac tttcttccaa
     1501 ctcgtgccat catgtccagg aatttgcccg cacttgccat gttaaagagc atccaacctt
     1561 cgtctattag caaaatcttt ggcacacttg tgggaagcga attgatcttt ttccaaatct
     1621 ttccaagagt caacaaaagc agcatggaga cacgctcctc accagatgtg cccctaaggg
     1681 aaatcacagt tttgtcctca agccttggtt cgcctgcaag aatctcgtaa attgaacctg
     1741 cgatgaggtc ttttaggtag ctttttgcat cctcgtccac cctgacatat aggtccttta
     1801 ttgatttgca accctgcgcc tttgcgcgaa actcttttcg gacaacgtca tccccttttg
     1861 agatgtcgcc aattatctcc gccgcatctt cctctttgaa tagcttgaag ggatcaagcc
     1921 caagattggt ctcctctgtt actttgacca cattaattga gaggaatttt gcaacagatt
     1981 cgtattcacc gtttggatcc acaataaaga cgtgcgcggt tggatatttt ttgattagtc
     2041 gtactaaaat tgtctttgca gtaactgatt ttccagcacc cgatgtcgca agaaccaaaa
     2101 tgttgtagtt gtccctttgg gtgtaatcat aaatgattgg tgcggaggtg ttgaggttta
     2161 ccccaagtgc gacgccgttt ggaacctcta tcatatctgc tgaaacaaac ggataaaata
     2221 ttgcactaga tccaagctca aaatagagat gctttccaat tccgtcttca agcatctgcc
     2281 tttgcatggt aattgtcgcc tcaaatgagc aaagcattga cctaatcaca gtcttgaaat
     2341 ttttgtctgc aattcttaat gcaagtgcgt cctttgcact tatcatcacg gccaatgaac
     2401 aagtaaacat tcttgtctct tgtcttgtga gggctgcaag caaccctgtc cctttttcat
     2461 acttgtaata agagtcagga ttttttgtaa tgcttgctcc aactaaccct atgtacctgt
     2521 taatgattga aacagctctg tgattctcaa ttggcttcat ccttattgaa accatttcca
     2581 catggggcag gaggttgtgg acccacgcag cagacaagct ggcagggatc ctgtataggg
     2641 tgtagcattt tgcaaagctg ccatcctcta ataccaagtg tgaaagcatc tctgatctga
     2701 ttttccagct tggctcagta gttgccatgg aatagtcaac tccaatttgc tgcaaaatct
     2761 gctccagagg ctctactgaa acaaggtatg ttcttggtac ctgcagactg cgaacctcat
     2821 cccccatctc aaactccaag ggatctttga gcattatgat acgaattggc ctttgaattg
     2881 aggctaaaat gttgaaaaac ttgctcagcg cgaccatctt tttatcatca gggagcattc
     2941 caaaattggt tggcttgatt tcgtagacaa acttggtttt tgtttgctga aggttttcta
     3001 attttagaaa tctcacatca taaaatgctt ggcgtctttt aaggtcggct atcataaact
     3061 acattgaaag gaagactcgt aagctttgct ctaattagcc accgagcaga tctgtcaaaa
     3121 atacaaatca ttgataccta caaggtaaat gatgcatctg tctacataac aaatgacggc
     3181 aagtatctaa tcgtagagcc acttctgacc aaagaatctc acgacattta ccaagagatg
     3241 atggagcaat tgttttactc actagagccg ttgagtaaga ctgacgatcc aatcaactac
     3301 attgaaaaac acatgtggaa ggaagcagaa gatcgtgcaa ttacagactc gttctcaaaa
     3361 tattttcaca gccttaggta ttatctgatc agagacatac tcggatatgg aattttagat
     3421 gttttgatga gggatgaaaa cattgaggaa ataactgcag aaagatttga tacaaaaatt
     3481 ggaataatac atcgcaaatt cagtgaattt aacattcttg atacaaacat ttcctttggc
     3541 tctgccgatt cgatgggttc atacatacaa aggttgatgc aaaaaaccgg aaacacggtg
     3601 accgccgcaa cgccaattat gaatgccatg acaaaggaag gtgataggat taccgtaact
     3661 tatgggcagg aggtctcgct tccagggcct acactggata ttagaaaatt cacacgggat
     3721 ccatttacaa ttacacatct gctagagttt ggcgcactga ctggtctgat ggcaagctat
     3781 ttttggatgc tttttgacgc aaaggccttt gggctagtag ttggggaaac tggctctgga
     3841 aagacaacca tgattaatgg ccttatggga ttatccaacc ctagatggaa aatagtcaca
     3901 attgaagaga cacccgagct acaaattcca cattatcgat gggagaggct gattacacgg
     3961 actagcccaa tgataaccca gtcaaatttt gacatcacta ttatggaact tatcaaggcc
     4021 tcattgagac tgaggccaga ttttgagata gtaggtgagg tgagggggga agaggcacag
     4081 ttcttgtttc aaagtgcagc aacaggacat ggaggcctga ctagctttca tgggtcaagt
     4141 gcagaatctg cccttaacag gcttgcctct gatccaataa acatcaaatc aagccaacaa
     4201 atgcttttgt ggtttattgc gcacatgacc aaactcaaga cggccgacaa aaaaatagtc
     4261 agaaaaattg tgagcgtcaa agaagtcatg ccaaagacag aatcggtttc attgcacgag
     4321 ctgttcaagt atgaccaaaa aacgtcatca tacaatattg acacaattga tcaattaatt
     4381 gaaaaaagca caaaagttca agatgcggca aacatactca atgtcgagcc aaaagaagat
     4441 ctgcaaaaaa gaattgattt gcttggtgaa tgccagaaat catctgcgca ttccgtcgaa
     4501 caagtatttt cagtaatttc aaaatactat gctaacaact taggctaaaa tggttacata
     4561 atttacaaaa ccactatttt ggccaaattg aatgcttttg agttaaaatg tataacactc
     4621 tgatctttga tttacattct cagtttttat ggaattttat cgtcatcatg aagctgtaat
     4681 cttgattttg ttgattcaga tgctttctca gaagcctcta ttagatgatg gtctgttgcc
     4741 aaatcaagag gaatcgtagc tggttttttc ctaaaaatta tgttaagaac attcccaaca
     4801 gttgttctaa ttgagaattt acttgtcaca aactcgctat cgattataga tgttttttcc
     4861 aatgttcaat tatggtgaat catgtattta tgttcatttg agttggtcaa atcctgaata
     4921 ataaaattcc attttgattg aagtgccttt tggaatattt gttgtcaaac catcgacgag
     4981 gctatctttg cctgctatcc tatttttgct tattactaga tgggattcaa gaggcttgat
     5041 ttctctaaat tctattcttt cggaacgact ccataagatt tcgagatctg gagttacaat
     5101 tgtaactgtt cttatgatgg aataacgacc gataattgtg gctttcaatt catagaatgg
     5161 ttttatccag atgtagttag cgttggtttt ttcagccttg atccttcttc ctaaataaga
     5221 tatgttcacc caattctgaa tgatggcgtc acattcgata ttttcatagc atggatattt
     5281 gaaaccccat ttcacataaa tctctcctat cgtggcattc aaactaaatc tgaactaatg
     5341 ttggaaacaa tggggacaaa ataatcgaaa gaacagaaac cagcgttgca attccgacag
     5401 ttatcgtata tttcatagaa aaatgaacaa gcttggacat gacaaggcct aggcttattg
     5461 ccgaaatgac gttgagtagg ttgatgaatc caatcaagtc attggagact aacatggcct
     5521 caatgttgaa tgcactttga accatctggt ctaggccagt gttcagtttt tctaaaatac
     5581 tggacatttc ctttgtagtg taagatatca taattggact cagaaaggca aaatacgaaa
     5641 tgacactgat gcttgcatgg gtctcttttt ttgtctgatt tatgttggtt gaaaaattag
     5701 tgatttgttc taggatttgg gcagttccgc caccactgtc ggcaattttt cctagcacaa
     5761 aaaatgaggt ttttgcaatc catgatctaa tcagcatgga atcaaaaata tctgaaaggt
     5821 ttacaccatg ctttagcctt gcagaaatta ctgagagtag gccatcaaag tatttgttgt
     5881 agtttctacc ttctgagatt tttattatgg catttggaat tggaatgcct atttttcggt
     5941 attctgtaat atctctgaaa aaatcaggaa gggcggattc aacaaaagag acttctctga
     6001 actgctttgt gcaaacaacg aaattaaaaa ttgccccagc agcaacacca acaccgatta
     6061 caaaccaagg ttgctgccct aacagtaatg agaccacgga agcaaaaata gctacaaata
     6121 cggccttgaa atcaaaatca attacatttc tagcccttgg ctgtgaaagg ctaatcatca
     6181 aaattatgaa aattgttaca actggaatca aaacaaatga gaggctcatt accacattta
     6241 ctgaatcctc ggcaagcata aacgacgaaa taaggatcat tgtcggaagt atggtcagta
     6301 ggatcaacat cgtttcgcca attatgtggg cctgtgactt gtagtttgca tatttgaatt
     6361 gcgccttgtg aaaaaactct tcgccctttc tttccatgta tagggcaagg ttgccgccgc
     6421 ttttactaat tgagacatat ccaagtagga agtttttgaa gtaaaagttc ggatgggaac
     6481 acccgtgctc gtttagcgca gtaatctggt ccatcccaaa aacctgcacg ttccttgaga
     6541 gcatttttcc ctcattttca attgtaagga aagttccctt tcccaacaca tcgataatgg
     6601 aattgtaaag tgactgtcct attgactgca tgacgcctgc atagagggta aagaaggcca
     6661 tttcgtcatc aattgatgac ttgcgttctg agacaataaa ccacaacttg agttttggat
     6721 aataaagcca gatggggcaa acaagtaacg caaaaaacaa gtacggattt acgaaaagtc
     6781 ccaaaacaac tgagattgga actaaaacaa aaagcgaaaa gatcatgtac gcaatactct
     6841 gctggactaa cactcttggg ttgtaggctt tgccagaaac cctaacctct tgctcaattt
     6901 ctgaattgat ttttccttga atggacgtga tagtcttatt tttcaagatt ggttttacca
     6961 tctccaaaat cttattattg gtacttaagc tgagtctggt agaatcgttt ttgagaattt
     7021 ttgatgatga aaatttcaag taggcaaaaa atatctgaag ttcattttta attgctgacc
     7081 agttaacccg taaaccaatt aacactaaaa gtgctgcaat gcttgaaaaa aatagcgcca
     7141 tgttttgcag caatactgca agccacatcg actcacaaac agctagaaat aatacggaaa
     7201 caaccttcac aacatcaaaa aaccagttct aaaaaaggat aaatttttct cactctagtc
     7261 ttcaatcagt tgcatccaaa aatcatacca ttcaatcgta acttccacaa acaaaatttt
     7321 ctgcttaact actttacagt ttttaccaat gatggcaaca agcaaactag gcaaaaaccg
     7381 acgcggaatc tcccccgcaa taacatcaat catattaatt ggggttgccg ttgcagcagg
     7441 gataggcggt tataccgttt atgcctcaac tgcaaacact gcatccctga aaggagctgt
     7501 gaccgtagaa gcagtaagcc ttgtaaaaca atcaaacggc gaggagtatc tctcaataac
     7561 aataaagaat tccggcaaca aggcatttgc ctctagttct gtaaatctac aaatagatac
     7621 cgattctgcc tccactggta ttcaaccttt ctcggcaagc cccttaccag cagcactaaa
     7681 tcctggacag acagcaagtg tcacggcacg actcatcaac tctgctggaa ctgcaattac
     7741 cttgcaaaac attggcgaaa caactcctct tgaaattgta gtggtaacaa ctgacggctc
     7801 tacaataagc gaactcatat ctgtaacaat gagcctatca taggccaaaa aatccctttt
     7861 attttttgta tttttagctc atgtgaagcc aattgatctt ttgtattttg aggatgtaag
     7921 cccgtttcat gaggccgttt ttacatttcc aataattggc aagctgagta tgcgccaaat
     7981 gtcaatagtt ggtttttctg caatcatttc atggagcatt taccaaggat cgggaaatct
     8041 actttcgatg atcccacttt gtgcgggcgg ctatgtaggg ctaaaaaaat tcaatgtcag
     8101 accaatcgaa ctccaaatta tttccgtaat caaattcatg ctttttggaa aaaaggctaa
     8161 acaaaaaaga aaatttacga aaaaaacagc cttcaaattt ccaagacaag aaagcaaaaa
     8221 acttggactc tcagaagtgt tccaaccaaa attagaaatc attggcaagg aaatcagggt
     8281 cagagagatc ttcaccgatc cactcaagcc gatccggctg caggtaaagc ttgagactgc
     8341 tgacagaaga ccaatagtca acacgcaaac acgagttgag tttgatggca atgtaatttc
     8401 cacacttgcc acaaacaaca atggagaaat cgaggtgctt gtcattccac aaactctggg
     8461 agaaaagaat cttttgattt atgcgaaggg ttttgagcag ccagtctttg aagaaatact
     8521 ttctattaag agtctgtagg attatgtaca ttaccgtttc gataagattg gttatatttc
     8581 ataattggta aatattattg cggttctcat tttgccgtca ggcaagtcct atcacgactc
     8641 gagaaccgta gtctcttttt cataatctac aagtccctta aatctactat ctccttttat
     8701 tatgatgcta aatcaagcag cacttgatca cgaagacaag caaaaataca tcagtattat
     8761 agtcgctgcc ggaattgcag gagccataat ttttctggcg caggatgtta tggagtggat
     8821 gataggtgtg gactttgaga gtcctgcaac cggcaacatt ccaccagcaa tcataacaat
     8881 gctcggaaag atcctaccat tcttgcgatt cattggaggt ggcttgatag tcgttgctgt
     8941 aataattgcc gtctcaaagc taagtcttgc gtctagacct gtcagatggt agcatgtcgg
     9001 ttctagctga cctaaaaact gctatcccgc tggcgggctc ccttgcactt tatttttatt
     9061 ttaaacctag tctcagttca gagacattgt ttttcttttt tggaatctgg atttcgtgtt
     9121 ttgcacttga tgccaaaata acggtttcaa accctaatct gataaatcat gaaaagaaca
     9181 ttatttttcc aattttatat cgcaaattcg gaccaaggct ctctccaata attcaattaa
     9241 tcattgaggt actcgcaatg gcacttgttg tgagcctgtt tgagagaaac ctaaacctga
     9301 tttcgatgtc tgttgtatca ttggtatttg gcttggccca cttggacgca tacctctcaa
     9361 acatcaaaac cataaggaac gtattgaaag actagacatc gtttagaata ttacaaattt
     9421 ttttttgagt tactctaaac atcctgttta aggccagcct tcgttccttt tatggagaat
     9481 tttcataaaa tttgtgaagt ttaagcgccg aatcaggttt caaaatcatc gtggagttct
     9541 agatgctggc ataataattc ttggtatgat tgttgcaatc attgcaattg ttgcggcgac
     9601 tcttgcttgg tctgatgttc tcgaggtttc atttataaaa cttatgactg agatgcctag
     9661 attcggttat gatgtccagt ctggacacga gataggaaac caaaactttg agatttatca
     9721 gattttgcgc aacattgcct tcataatcat gttgtttgtg ttgatgtttg cagggctttc
     9781 cttcatgttt gagcacataa acttggtgcc gccagaaaca ggatttaaaa ttatttcaaa
     9841 aagcttgttg tatgttttct ttttcttttt tttcccaccg ctttgggact tggtggcagt
     9901 aactgtcgaa caaacatcat tgtggattct aaatccagag gatcagacca tgccaacaaa
     9961 aaatgtcgag tttctgttaa ctaaactcgg cagtattgaa tcaccagaat ttacacttga
    10021 tgccatagtt gcaggaattt cagatccttt tggcacgctg aaaaacatgt ttttgagtgt
    10081 ttttttggca actttcaagg caattgcgct tttgattttc atgttcactg cttttttgtt
    10141 gggaacaatc cgaatagttt tgacagcaat agtcacaatc gcaatacctg tgacattgat
    10201 gctttcacta atcccttttt tcaaaagggt caccaacagg ttcactgatg ccctgctagg
    10261 cttgatgatt gcccctattt tttctgccct ggtaataact gccggcgttg cacaccttaa
    10321 gacacttgaa tcaactgccc aagatcctgc tgtggaatgg tttgcagccc tggccgtgat
    10381 ggcccttgct acatttattc cggcaatgat tgtaccactt cttgggtctg ttatgggctc
    10441 agtttcttca attactggca gggctgtttc gacaggggcg ataatgacta atatggtagc
    10501 atctggggtg gcgcgaaccg taggaggagt gatcaccggt atgacagatc cagttcgcat
    10561 ggagacatca actaatccat tgaatcttgc aaaattggta ttctcaggat ctaggttttt
    10621 accaatggca aagactaacg ttgagaaaat agggggcgag atttctcatg ctggaacaat
    10681 gcatggccca ctttctggga tacacagaat ggacgaaaac ttgtctagag aatcagcgca
    10741 agacttcaca ctttcacgca atgtggataa aaaaagtaaa tccgatagcg caaacgagat
    10801 ctagtcctca acatgcataa tttccatgtt taattcctca tgttctgaca gacacacttc
    10861 tgctttagtg catcaacttc agaaacagaa taagcaaatt ctttgtttgc gacagtgtag
    10921 gtatcaaaca cttgttttgc tgagccctcc accttgaatt cataatgtag tctatgatca
    10981 tcttgcgccc attctatctt aaacgagttt ctgccagtgt ttttgtatgc tttgaggatt
    11041 tcaaagggca agtctaaaac tagtccgttt ctctgagttt ccaaatatgc tccaaagttt
    11101 gtaatgtaaa aaatgccctt tccgtgaagg ctttgtttta cctcaaaagc ccttgcgatt
    11161 ttttcaccca gtctgaagtt gatcatagga ctagctagaa aaataatcaa ttctttgctc
    11221 ggaatttatg tcgaatcttg cggcttctat cttagttgtg aggaattttt ggctcattgt
    11281 gggtaacaga aaccatgtct tttgaccttt gtaggggatt tgtactgcgg gaataccaaa
    11341 ttttagtacg actccttcgg gttttgccac tatcccatcg tcttttgtcc ttatcctgta
    11401 ttctatctga tgtggtctgc tttggagatc atttacttcc ttgaaaaatt tattgtgtgt
    11461 gatgtagaga ttgcgctcct tgtcaaacca gcaatcattc catatttgct cactcaaaac
    11521 ataggaggga gtcttttcga atcgaggttc tatgactgga ttttggatgg taattttttc
    11581 tctggcctgc tgttcgattt ttaatccgat tgtccttacc gcatttaggt aatcttcatg
    11641 gatgcttttg tatctggttg taatctcagc tggcttttca tagttgaaaa caaaatcgta
    11701 tgcactatta ctttccttcc aaatgatttt gatggatttt ttgtcaattg gttgcaagct
    11761 taggatggag ttgtgatcaa gctccaatat gacaccatta gagttttcaa ctagtattcc
    11821 aaaattgctt atgtaaaaag tagacctacc aaacttgcag tttattgcaa atatttcttt
    11881 gatctgttct ccagcaagaa gcgatttcat gcagtcaaaa ttttaatcgt atttaagacc
    11941 acatgaaagc caaaccaaat tgcttctcct taaatgatat gaagtcgtga tcctttatgt
    12001 caagcccata tgttattgcg gcaataagtc tctcttcgat tctggctctt gctgcaataa
    12061 ttgcaacatc aaatggggta atgaccaatg ctcttttggc ccaagaatca atacaaatcc
    12121 agacacaaaa aaatcgtgaa aacttggaaa ttgccatttc taatgatgtt ttaacaatga
    12181 aaaataatgg gttggattcg ataactatca aggaaattag gatttactct gatgctgaca
    12241 ccacatcact acttgcgagg atcaaatttc ctgaatctgg cctgcagctg aaacctcttc
    12301 aaaaatcaag ccacaatcct tcggaatttt ctgttgccag ctttttgaca acgaccattc
    12361 ttggaataac tgatttggga aatgtctttg ttgcaacgaa tctgttggct gaaaactcgg
    12421 ggaatggaac aggaaacggg gaggcaatga ttaatgggat gggattaaat tccagaatag
    12481 ttacgtatga ttattctgga aagctaacgc acggatatgg tgattctgga acgctaagct
    12541 cgttaaagac gtataatcaa ttttcaacta tgcatgattt tgctgctcaa ttactttcta
    12601 ccgattccaa aatcaccgtt gcaattccac aatttaacac agaatacgag tatgttcctc
    12661 aaacccaatc tgttcaagca acgggaacta caaatcctaa cactcttggt tattctcaat
    12721 caaggacagt tggtggatcc gcaatcactg ctgtgggaac tgacggaatt acaatttcag
    12781 gaaccggcat tgtcatcttg aaattaaata attatggtga tcaaactttg gtactagaag
    12841 gaaatgttcc aagtggtggc ttgctgcaag taggaactga tctacaatat gattacatca
    12901 acataccgta tgataacgca tatggtttca gactatattc ggggggtttg ccaggtgcat
    12961 cttattcgca aagcccgtgt agttatgcaa acggtgttgg ctacaactgt tgtgcgtctt
    13021 ggacctatac acaatcattt gcatctccac tcatactgac cacatcatca aactattact
    13081 cgtactatta ttctgcttca tatccattct caatacttga cggcactcaa aatccaacca
    13141 catattcact aaactctata cggatgactg caggtgcctg tagtggatgg cattcaccct
    13201 atgggagctg gcctacaaca tcacctgttc cttctggtca aacccttttt gtattcgata
    13261 aagcatatgt catgaacaca aaaatcgatc tgacttcaac tttccaaatg ccgcattcct
    13321 tccaagctgg aaaacaatat tacatatttg caaaaccaaa cggcggaaca ataacgatca
    13381 aaggttcttc tttcaatcct tctatgactc cttatctaaa aattacagac ctgcccccaa
    13441 acattccata cgaaatcgca aaggatggac ttctagcggt gagtggcatg gctgacagta
    13501 tcgggaccgt atctctgaca ttagacgatg tcaaaatcgg tggcacgaac ccaaacggga
    13561 acttgtatct ttacccagac tctttgaaat accgaggttc cttctcaaca gtggtttttg
    13621 ataacgtaaa tgaccagaca atccacatag acacccctga agacagggtt tacgttactc
    13681 acgcatacgt acaaattcca gttgtcggtg atgttgaagt aactgacaca tatcttgaca
    13741 atcatctctc actttcatat ttgaacggca actatactac cggcgagagc atcaaggtcc
    13801 ctgtcatatc tgggtaccat gatatcaaca tgaagataaa caatgtccaa actatgacag
    13861 taattgcgaa tgttcttggc ggcacaggac ttaaagtaat ccagccgtct gcatccacca
    13921 taacacaaca aaatgacaac aacctcgttt catcaattac agctacgaca ggatctgtct
    13981 cgtatattat ttccacgtct tctggaatcg tcacaacatc aattactgcc acaatttcag
    14041 gcgactctga aatcaaaaac catgcgtatt ttggggctcc accaccgtct cctccgtccc
    14101 ctccaccaag agatccactc aaggcgtggg ttgatgtgta caaaaacgga gtcttggtaa
    14161 accaacagca gatttacttt aacgcacttc caagctcgca aaattctgga ggggtgagtg
    14221 ggtcctcctc atacgtgatt gacaaataca tgtatcctca gactgtcgtt aacggcgtca
    14281 ttacaacaaa tgttttgcca ggtgattttg ttgaattata cttgtatgca aacattcagg
    14341 cggacggctc aacgccgcca attccatcag gccatgtttt ttaccatcat tccggcgaag
    14401 ggcatgcaac taccactatt catagtggaa gtattttatc gtcgtaaagt cattttatga
    14461 cgcgagtcat atccagctca tcaatctgcg cctcgattgt ccgattgagc gcctctttgt
    14521 gctctgggca aaaccagaaa tacccatcaa tagtctcaaa gcagccgcaa atccactggg
    14581 tttttttgtc gccctctact gtgagcttgt gatagtccgg ctctgacatg atacgattac
    14641 caaaaccaat caattaaaat gtattaaaaa taaaaaatta aaaaaaagaa tctatgggca
    14701 gccttccatc ttttggatga aaaagccgtt tgtcttaatt gcgtcctcta ctggctttgg
    14761 cgcgccctgc gagtggcaga actggcactc ctcagtagtt acgcgcgcat ctgcctcgcc
    14821 gattgagccc agccattttt tggcatactc gattgccttt gtgtggtctt tttctgatgt
    14881 gatcacatca aagtgcatgg tatggccgtc tttggcctct acataagtgt cgtagacgtg
    14941 tatttccatg aaataataat aaaaaaaggg atatttaatt caagaaatac atgcactctc
    15001 tggtttttga gattccaaaa attgataaca tcactctaga actggacgat tctggcacat
    15061 cggggcttat tttattgcgc ctcccattta ctgttaaaat caacatctgg ggcgaggaga
    15121 tctatacgga accaatccca gtcagggcag aagaagaaaa cgcaaaacct gtagtggagt
    15181 tgtttgatgt ggcgtactgg cctcctgggc aggcgctgtg cctgtttttt ggcccgacgc
    15241 caattagcaa ggacgagatc agaccgtact ctccagtgaa tgtgattggc aaaataaaaa
    15301 accctgacaa aaaaatcctc tcaaaactaa gagatggaat gaaggtcaca atacgccttg
    15361 ccggcttggg ctgactatat gttcaaaaat ttccgcactt ttatacaaat tttgcaatta
    15421 catatcatga acagacatga taccatgagg ccggaatgcc acaaaatgga tctagatctg
    15481 ctaggaactc actcaaatgg aaccaagttc tatcgctgca aaaactgcgg ttatgccggc
    15541 aaagaaagcg cttttagcgg agattttcat atttcccaaa tacaatgact gcatcaataa
    15601 ccaaatcgga agccttgtca agtagagaga ttatgttttg cagatcctca atttcgcgtg
    15661 gattacttat tgtaatgtct gtttttagta gctctactgc atccatcaaa tctgcaaagg
    15721 caccaagtgt tttatcaaac gcctttttca tcacatcaaa aagtcttaaa gcataaaact
    15781 tggtaaactc caggcaaaaa ttctctcaaa gataaagaaa tgaaggctac gatacgtctg
    15841 gcttgattcg gttgtgatct taaatctcat tgaaccgatt tttggctgct aaaatttctg
    15901 cgcttttata caaattctgc aatggtgtgg tatgaataca catgagtttt tgcgaccaga
    15961 gtgcaagaaa acgaatctag aattactggc aacgcactca aacggcacca gattctaccg
    16021 atgcaagagc tgcggctttg gtggcccaga aagaacattc accgacgagt ttcatgtttc
    16081 ccaaatatta caacggcatc aatgacaaaa tcggcagcct tgtcaagcaa gaaaactatt
    16141 ttttgcatat cctcggtttc ggctgagctg ctcaccctca tctcggcttt tatgatggct
    16201 atcgcatcaa ccagatccgc aatgacgcca agtgtaattt caagcgcctt tttcatcaca
    16261 tcaaaaagtc ttaaagcata aaacttggca aaacctggaa aaaaatcaaa gctaaccgac
    16321 ggaatgaagc cacaatccgt ctggcataaa ccacaccgtt tctgattcta tttgagtccc
    16381 atatcaatta catattctct tggcgaaaga aacggcatcc cagttatcct ggacagctga
    16441 gccttcatgc gaagaagctc agtatctcct gtgacaaacg ccccaagctc ccggttgttt
    16501 tgtttggcgt gtcttgctgc tgcaacatgc atcaggtcca gcgttctaag tgtagtctgg
    16561 atgctcaaaa gtatcgattc gttgaacacg tttggcatgt ctgttttgat gtttcccact
    16621 gcaaagggtg tatcgcctga catgtggaca aaccttatgc caagtcctgc aagcgtcttg
    16681 agggttttta ccacgaacgt ctttcttttg tggtcatcct tgctgccctc ggcacgatgg
    16741 tacaggcggc tcgctacaga tgcgacctct agtaatgtga gggtggatgt ttctgcctga
    16801 atttcgcctt tgcgcagcct gctggcaacc ctgcctgcct cggcgtggta tgggtcgtct
    16861 gacttgagtg ctgatatgaa tacgttagta tccatgtaga actggtgcgt cttacctgtc
    16921 ccttccttga gccactagct gagcggcagt cttggcaccc ttgaatactc tggctgccag
    16981 cttttcaaat tgctggtcgg acatgtagtc ggcctctctt agaattcctt gcagctcgtc
    17041 atggccgaag agctctgggt gttcttcaaa gtgttcttcg agcatctttc tgacggcctc
    17101 gcttttgttt ttgtaggcct tctttttgat gagtctttct agcttctcta ttgtgtcctt
    17161 ttcaactttg acgttgagta tttccattat ggtaatacaa tggtattacg taacataaaa
    17221 gtattactag ctagatgccc taactgcggc ggtttatgtc attaaaaaaa tgcctttatt
    17281 gggcattaag gccaatcata ctgtgactga aaaatctgat ttcaaaaaga agctccaagg
    17341 tacgatccaa taaaacctac gacttggaag ggactgattt ctaggccgaa cagatagtta
    17401 tttattattg aaagtggtat tattacttag ttaatgaata ttaaccaagt aaaaatgggg
    17461 gttttatgag caccgaatcg gatctggcgc agagggtgga aaaacacttt gccaaagaag
    17521 gctggagtgt gagaagggaa gtaaagatca ggggacggat agttgacatc gtggccataa
    17581 aggacggcca gatagcagca gttgaagtca aaggggcagg cggcgatgtg gaaaaaggga
    17641 tcatgcagtc gctccaccaa aagggggcag ttaacctgtc ctatcttgcg ataccgcaag
    17701 atacgctgac tgattctatc aaatctacat gcaggaatct cggaataggc ctgatcctag
    17761 tagacaaaaa aatcaaagaa gctatcagac caaagcacac ctcgtctctt atttccgtca
    17821 aaaataagat tcttggcaaa gagtcccaaa agcgtagtaa ggtcatagaa tcgaagagcc
    17881 cattggagat aatatttagg acaaaaagcc agatacttgc actcaaattg ctttttctga
    17941 acacaagcaa agagttccat ctcaacgaaa tagcccgcag gaccggtctt acccccccag
    18001 ctgtctcaaa ggagctcgca aaactagtca gggcgggtct agtcaaacgg cgcaaccagg
    18061 gcaacctctc attttacaag ataaacgaag ctggcataat atttggcgag atgaagagga
    18121 tatttctaaa gtacgagata ttcagcgaga ttctatcccg ggagctagca aaagagaaga
    18181 taaagtacgc actaatctac ggctcgtttg ccaaagggac ggaaaaggag ggaagcgaca
    18241 tagacctgct agtggtaggc gacgtctcag aggattacct cttaagggtg attccgagga
    18301 tagagcgcaa cacgggccgc gagatcaact ttatactttg gaccgagaag gagtttggag
    18361 acaaggtgca gaaaaaaata ccgctgattc gtgagatatt agagactccg ataattatga
    18421 ttgttggtga cgaggatgga tttaagcgag ctcttaagga aaggattggt tgaggagttc
    18481 aagctggggc cagaccaggt aggaaaggca ttggagaatg ccgagaagag cctaaagacg
    18541 gccgaaaata tattgaaaat agaggaatgg ggggcggcac attcttctgc ttacacggcg
    18601 atgctccaca caggacgtgc gctcatgttc tctaagggat acaggccaag ggcgcaagac
    18661 caccatgtgg cagtagtgga gttttccaaa atttacgaga aaaagtatac tgccgaggtc
    18721 ctagcggcat ttgacaaagg caggaggcgc agacacgagt tccagtacga cgacgtagat
    18781 gccatatcgc caagccaagc cagaaacata gtcgagagtg caaatgcgtt tgtaagcaag
    18841 accaaagaaa ttttgaagat gtgatcattt gctgcaaaaa cattctgtaa tttgatggaa
    18901 atcttttgtt gatggcagaa agtgcttgat cagcaagcca tctttgaaat ctttgatttg
    18961 gatgaatcct taattaaaac aaaccagtat tttttgccat gagggagagg cttctgtggc
    19021 tttgcacgtg cactggaata gaagtcatga ctgccaccgg cgtgacggca taccttttgt
    19081 atttgggcca ggccacaact gccataatct ctgcaatcat atttgcaaag ctaatcgcat
    19141 ttcattttgc catagaggtt cacgagagac taaaacgcaa gcgacaagca acaatcataa
    19201 aatcttaatg tagagtggtt tgcctatatc tgtaattgag tagttctggc aaccaaattg
    19261 acgtcataaa aaaattatac ggagagaaaa actggcagga aataatctcg ttttgcaaaa
    19321 aaatgctaga agaggaccca aaggacctag ttgccctgca aaacatggca acggcgctag
    19381 tcaatgtggg aaaattcgat gaggtcatat catgctgtga tacggttttg cagatgaacg
    19441 ggactgatga gtatgcgcta aaaaacaaga tctttgcgct agaaaagctg cgccgatatg
    19501 atgatgtgat atcttgctgc gatttgctct tgaaaaaaaa ccaaaacgat acttggtccc
    19561 ttgacagcaa gggcctggca ctaaatgagc taaaccggca cgatgaggca atctcaaact
    19621 atgacaaatc cctgcagatt gatccagaca acacagtagc gctaatgaac aaggcagtaa
    19681 ccctttcgtt tctgcaaaga tatggtgagg caatacagtt ttttgacagg gctcaaaaaa
    19741 tagacaaatc aatcaaggag gcagctcttg caaaatctga cgcatacaaa aagatcggac
    19801 gggaaaatga ggcgtttttg gcagcacagg ggctactagt agaagaaatc gagggactgg
    19861 tactggaggc aaggcaaaaa aagacgaaag tctttgacct ttactgtctt agggagtttg
    19921 agggactaga agaacgggag aaaaaacaca acgaaaaaca ggattcaaaa tcaaaaagct
    19981 agcttgcggg cttgctaaac tgtataataa tatcctacct tcgtaaaggt ttttgagctt
    20041 gtatgttgca ttgtttttgt gctcagggca aacgctttgt tatttgttgg actggccttg
    20101 attttatttg gagcgtttaa tctaacaact gacgcactag cacaggattc cgacgttgaa
    20161 caaaaatctg gtgatcaaaa tgacattgta gacactatac taatcaacct gatttcacaa
    20221 attggaccaa tagttgcagg ttttgtatcg attggaatcc agtttgcgcg aaaacagggc
    20281 ttgaagataa gtgccgaggc agaagagtat tttgtaaaat cagcaagctc ctttgtcgta
    20341 aaccaaggaa ggtttctgta caaaacaatt cgtgacaacc cggaatatct agatgatttc
    20401 aaaaagggtg tagttccagc caagctcaaa gaggtaacac gcgacaaggt aattgagcag
    20461 cttcaaacag agctgcgctc agacgaattc acaaaggcag caaagtcaat gctagaagaa
    20521 aacctggtga ctctggtgga aagatccttt actgagcaca aaacccacat ggcagaaaaa
    20581 acccaatcaa tgctaaagga tctagtccca atagcagtaa atgccacact tttggggttc
    20641 aagagtccag acgaggtaac aaaacaaaaa gaaaagataa tcgaagatgt tttagaagct
    20701 gttaggaaaa actttgatct tgaggacatg cttatgccgc aagacatagc aacaacgcac
    20761 gtcaaatcag agctaaacaa gattgtcggc tcacttaaaa caccttagat gattctggct
    20821 ttagctgatc tcaattattt tttctttaga tttgtggcct gactttatca caacacttgc
    20881 agacgagacg ccaaaatgtt ttgcgagctt ttttattatt tccttgtttg cagctccacc
    20941 gaccggtctt gatctaacac caattgttat ttggtttcca tcaacttgaa caaaatcctt
    21001 gttgaattct acatgaacgt gatacaacaa aaccggaaaa aaatctaggc gtttactatc
    21061 cagtcgtagc cttcttgctc tagcttgtcc gcaagtgatg cgtcgccgct cttaattatg
    21121 cgcccatcgg aaaacacatg gacgtagtct agctttttga ggaacttgag aattcttgca
    21181 tagtgtgtta tgacaattgt tgtggcatcc gcactggata cttcgcttat tgcctttgcc
    21241 actgcctgga ccgcatcaat gtcaaggccc gagtccggct catctaaaat tgagaccttt
    21301 ggctttagca ctgccatctg cagtacttcg gatcttttct tttctcctcc ggagaagcct
    21361 tcatttaggt acctgcccaa aaagtcgtcc ctcagtccca ctgccgcaag attttttttc
    21421 agataatttt gaaactctcg tacggtgatg aacacctccc tgttgctgct ctggagagat
    21481 tttgataatg cattgtatga tgtcctaaga aagtgcgaat agccgacacc tgagacctct
    21541 gtcgggtact ggaatcccaa gaacagcccc ttttttgccc gcgcatcagt tgaggcatgg
    21601 gtgatgtcct ctccatcaaa tagaattttt ccctgggtta cctcgtattt tgggtggccc
    21661 aagagcgtgt acgcaagggt gcttttgcca gagccgtttg ggcccattat tgcgtggacc
    21721 tcgccaggac cggtcttcaa atttacgccc ttgaggattg gctttccatc ccttttgacg
    21781 tgcaagtcga ttatctcaag tactgccatg tgctcaaagg tgtttttcag ttatataatt
    21841 ccgactgaat ttagctagtc ctaagaaaaa caaaaagagg gggatgttta gtttttggca
    21901 aaacttggtc gtagtgttaa tcgcatcttg tagcacgtaa ggatctcttt gcacctgttt
    21961 cgaatggtta cctcagttac gcctgcgacg cttgagacgt cgcgctgaag gacgttttgt
    22021 cctaaaagta ttgaggcaat gtacaagtac gcagcagcaa tgccgtttgg cgccttgccg
    22081 tctgcaacgt ttgtgtcgct tgtcttttcg gcaacctcca aggcaagcct ttcaacacgt
    22141 acatccgtgt ttgtcagatt tgagatcttg gagatgtact tgtccataga tatgactggt
    22201 gcagttaccg atccaagctc cattacgagc attcggtagt attttgctgc aagctttgtt
    22261 ctcatcttga tgtccttgtt tgagccggct ccactacaca cttcttctag tgatcttact
    22321 acatcgcact gcttgcacgc catgtagatg gtagcagatg caatgcatgc aactgatttt
    22381 cctttgactt ccatgtagct gtcaagattc cgataaatca ttgacgcagt ctccagcaca
    22441 tttgcagaca acgaaaggtt ttggcatgcg cctccaatta cagagagcac gtttgcaagc
    22501 ctcctctctc ttggcgttga gattcgtacc cgttgttgcc attttcttag gctgttcatc
    22561 tggtttgcta cctggtagtt gatcgtcttt ccggagtaat cctttgtact aattgagatc
    22621 tccgttgcga tccccaagtc gtgttgcgaa taagtggtct gtccgctcgc ccttgtgttc
    22681 ttcatttttt cttcaaggct tgagctcttt gattctggac cataatctgc cagctgttcc
    22741 atgacgacaa ttccgcacat ggaacagatt ctttctccgc tctgaacgtc atctatcaga
    22801 gatgatttgc attcagggca gcatgtttct agttctgtga cattcatctt tttttcttcc
    22861 ttgttttttt aatcacaggt tcatcaagga agtacacttt ttttccggtg tattttttta
    22921 tgttgtttgt cagagaaatc gccgaagcat acggactggc aactgggcca atcagctcgg
    22981 tgactttggc aatccttctg ccggtattgt cgcaaacaaa gttgccttca tcaagtattt
    23041 gtttgagtcg tatgatgact ctgccactac cggccaagtg tatgatttcc cctatctcct
    23101 gcaattattt tctaaaatgg atgtctgcta atcatagagc tctgtcaact ttactttagc
    23161 tactagctag atcgaatttt atttcgatga cttggcccgt cttgccacaa gtttttctga
    23221 aatcttgtaa agaatttttg tcttggcgcc gaaatttttt ggcaggacga catacccgga
    23281 cctgacataa gcccgtcttg gaaaccggac cttgtcttcg gattcttttg gagaaaaccc
    23341 agcttctact gatgcatcaa ctagttcttt gagtgacgga tcaaaaacac atttttcttt
    23401 tgggagtctt cggcccattt tttttgttag cgtcttgttg aaataatcca accagattac
    23461 gacgtgttcg taatctttca tctgatcact ttatcaaaat tgcatttact actccgtgct
    23521 gtcctggcct tgagacaaca cggcacttgc cgtcctctgt ttccaaaact gcgcccatcg
    23581 atatcactcc gcgcctttgg taatcgttgt ttgtcgcatt ttctaaaact ttgagaatct
    23641 ttgatttttt taccttgccc tcagctagtg ccagattaac atgatcaatt gttttgagtc
    23701 cggtcttgat atctccaccg cggactgccc tagatactgt cacctgctgt cctgttattg
    23761 cctcgtttgg aaatctgtcg atttcgtatt ttctttttgt tcgaagcggg tgtcttcttc
    23821 cgccggtaat cttgcttgtc caaagattct cagttgagcg tttcacaagg tgggtttagg
    23881 tcactctaat ttatagaaag cgcaagtact gtgctaattt tgctggggga tttgtgcttc
    23941 ttgcactact tgctgtgggg cagcattctt tctcattttc ttgaagagaa tctgtttgag
    24001 aatatccgag tctccgctaa ttccaaaata tttttggaac ttggaagtag tcatgtaaat
    24061 tttcagcctt ccaacatttt ggtgggaaat aaaatcaagc tgcctgagct ctttgagatg
    24121 agaataaact gcagtgcccc tcgtctcgac tagctgcttt gatgatatgg gctgcatgta
    24181 tgcaatatat gacaaagtct ttagcgttgc ctttgagaga atcggctttg atgcatacct
    24241 tcgaattata tcgttgaatt ccggcctgag ctgaaatacg tacgagccgt ccggaagagt
    24301 atctatttcg agtgccttgt aggccgactt tgtccttttt tcaattcctg caagaagggc
    24361 aagggtcttt gtccttgatt ctgtgcctga ggccctgatg agctcctcaa ttgagagcgg
    24421 ccttcctgcg gaataaagcg cagcctccag tcttgctatt gcctcagtat cgtcttctac
    24481 tttgaccatt ttatgtgagg tcctcccgga tgagggttat tttgatgtcg tcttcttgct
    24541 gctccaagtc gactttttgg tcctttgcca aaaacaagac ggcaaaaaaa cacctaatgg
    24601 agtctgtggt atccaagtct gcaattatgt ctttgaggag gccaaagcct tgcacttcaa
    24661 tctttcttat gatcagctcc tcgtatttct caatgatgtt ttcaagcgat gtgaaataat
    24721 ccttaaagtc gggaacctct actggctcga actttagctg gccgccccgc ctcgatcgcg
    24781 ggttggcaat agtgccaatc aggttttcca ataaatccat cagctcatct agtgttacgg
    24841 gatatgttgc ctcgtgtctg tacgggatgt tgagcagctg tatgtcaacg tcggtcctgc
    24901 tcctcagcgg ctttttctcc attgctgcct tttgcagggc aaagattcgc tcaactttca
    24961 ttctgtgaat cagggatgat gagagtgctg ccatgccggc aacgcgcagg tcttttttgt
    25021 cggccttttt taaaatttga attaggatct cgaggatttg tactatgttt atctcccaga
    25081 catccttttt tgcaatggtg ttggggttga ataagacgtt tactggagct tgagaaatgt
    25141 tttctgctgg ttcgctcaca actacaactt gagccttact caataatact taggtactct
    25201 atttttttgc ctctcccagt caactcttat attcttattc tgattgacgt aaagcagatg
    25261 aaggcagcca gaatcgtaga gccgcaaaaa ccacttcagg tcttagaaat cgagacgcca
    25321 aagccaaagg acgtcgaggt attagtaaaa gtaaaggcag tcggcgtctg ccacagcgac
    25381 ctgcacttgt gggaaggagg atatgatact ggcgatggtt ttatgaaagt aacagacagg
    25441 ggcgtcaaat tcccagtaac gccgggccat gaaattgtag ggaccatcac cgaagtagga
    25501 ggtgcagttc agggatttgg tgtaggtgat gatgttttgg tgtatccttg gattggctgc
    25561 ggaatctgcg ttgcgtgcag gattggaaac gacaacctct gcgatgcacc gcgttctttg
    25621 ggggtctttc aggacggcgg atatgcagaa tatgtccttg ttccacactt caagttttta
    25681 gcaaaggtct caggaatcga gctggaatct gcggcctcgc ttgcgtgttc tggtcttacg
    25741 gcatacactg caataaaaaa atcaggtgcc aactcgcagc acaacatggt catttttggc
    25801 gcagggggcc ttgggctcat gggggtccag attgcaaggg caatcacaaa tgccaatatt
    25861 atctgcgttg atatcgacga ggcaaagctt gcggcagcaa aggagtttgg cgcagatcac
    25921 gcggtaaact ccaaggatcc agaagcagtt caaaaaataa tgtcaatttg caatggcaag
    25981 ggcgcagagt gtgtgataga ttttgtaaat gccccgccga cagtaaagat gggacttgca
    26041 gtgataagaa aaaggggcaa catgatccta gtcgggctct ttggaggctc tattgagata
    26101 tcactagtca ccatacctct taaggcaata acaatccagg gtgcatacac tggcaactat
    26161 aacgacatgc tggagctttt gggtctggca aaacgcggcg tcataaatcc aatcatatca
    26221 aagcggtatt cgcttgcgga tgcaaacaac gccctagaag acctaaaggc acgcaagatc
    26281 ctaggtcgcg cagtaatcaa cccgtagaca aaacaatttt gctttttgct cgtaacgcag
    26341 ctctgctact atatagtgaa atatagtata tgtagaacag actttaatct ccgtaaactc
    26401 ggagcaagct tatggggcaa tggctttcct gggttaaatc aaatgaaaag gaaatcctaa
    26461 caattttaga taatcttgca ataaaggcaa aagaaactgg cgaacagcta gtcttgttgt
    26521 tttcagacat gaacaaggct tcggagtata ccactaacat aaagaaacta gagcgccaag
    26581 cagatgatct gaccagatcc atttttgcgg agctgaacaa gaccttcatt acgccacttg
    26641 acagggagga catccaaagg attgcttcaa agactgacga tattattgac tttatagaag
    26701 gaatcgcagg caggatcaaa agctacgaag taactttgac tcccccgtac atgcttgaaa
    26761 tatcaaatga gctgttaaac gccatcaaag aagtggagat tttgatctca aggctaaagg
    26821 gactcaaggc agacaaatcc ctagttgagc attgcagaaa gataagcgat atagaacata
    26881 acatcgacga actgtatagg gctgcggtgg gtgagctttt caaaacaaat gatgcagtta
    26941 acataatcaa gctaaaggac atctacgagg caatcgagca agcatctgat aggtgccttg
    27001 atgttgcaga cgtagt
//
LOCUS       DASWKH010000084        12063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2083754, whole genome shotgun sequence.
ACCESSION   DASWKH010000084 DASWKH010000000
VERSION     DASWKH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 12063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12063
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083754"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VGA92_06635"
     CDS             <1..364
                     /locus_tag="VGA92_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEX9846126.1"
                     /translation="DGMLAMAIPVVQRPTSIIKLGILAMTWSSEHYSVDFVKSLIKKQ
                     GFDVVSLEKIGSSVYEPLTDYYAKNRKSLKPKILKQYPSYVEKILFNSLHKMKKVSED
                     KIIDYLLIVCKKQEANPN"
     gene            complement(368..865)
                     /locus_tag="VGA92_06640"
     CDS             complement(368..865)
                     /locus_tag="VGA92_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846127.1"
                     /translation="MAENQIVCKNPPEDCQIINASLIKCILVPFDNSQYASRAFAHAL
                     DLAKKYGSMLVIVAVSNESDKTQWVNETPSRQRGVGKSRNEEFKEIFKKLEFQASRFH
                     VKCESLILESNLIAEAIISFATKKHVDYIVMGTHGKGQAKEMMLGRVSTSIALNAHCP
                     VVLVK"
     gene            1104..1667
                     /locus_tag="VGA92_06645"
     CDS             1104..1667
                     /locus_tag="VGA92_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HEX9846128.1"
                     /translation="MPQTKPIVRIENVVASATVDQKLDLKEITKKFPDTEWNPEQFPG
                     LVFRIKTPKTATLIFSSGKMVCTGGKSEDMTVKAVRTVVQQLRKGGIKIKKEAIVTVQ
                     NIVASANLGGKIHLENAARVMPRSMYEPEQFPGLIHRMLDPKTVILLFASGKLVCTGA
                     RKESDVYRSVNALHNTLEEKGLMIYDS"
     gene            complement(1664..2272)
                     /gene="pyrE"
                     /locus_tag="VGA92_06650"
     CDS             complement(1664..2272)
                     /gene="pyrE"
                     /locus_tag="VGA92_06650"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9846129.1"
                     /translation="MEFVKEFATFLHQNGIIKFGDFTLSSGKKSSYYVDLRLVPSYPH
                     QYRRMIKYLQNLISEQIGFDNFDAIASVPTGGLVIASSLAFEIVKPLVYVRSEVKAHG
                     TGKLVEGIVKNGMKILVVDDVATTGGSVINGIKALKEAGASISDVYVIVNRLEGADGA
                     LKKEGATLHHLLDILEIAKILHEYNLIEKSVLDKVQKQTEKS"
     gene            2323..3408
                     /locus_tag="VGA92_06655"
     CDS             2323..3408
                     /locus_tag="VGA92_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9846130.1"
                     /translation="MPEIWLNYGTTDVVLDIKAENLDQKVEFESQTLADSAVSEKLDA
                     VDLSKPLTFVVLNYTGSVQNALSVLFEKCTQKGVPRPKVFADRQIMNLVKATLPPESQ
                     VQAFEGIENVNPDLVFVGEMEFDGLFGFDSVCTKILRRFGTEQMLSAYEKRQGSLPNP
                     GKETPSLEVAKNFANGFEISAIEIVASPKGIVDLSVGHPSKTLEISKTMGSFTKTIEK
                     HRTLLISTGKDASNDTLGHSLGSLWNCYSAIRDEGLAILLGECKGGISSEAIRQYIEG
                     RMSIERLQKPAKYVDGMEGLLFLSEIQKKIKIGIVSVLPEFYIKKLDMILFDGVKETL
                     EYVLKHQGARQKISIVPDGARILLGPN"
     gene            complement(3394..4473)
                     /locus_tag="VGA92_06660"
     CDS             complement(3394..4473)
                     /locus_tag="VGA92_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9846131.1"
                     /translation="MMEPTQEQVISIVSSVFEVRDVIISLEKMEFEVADLDFKEKFVS
                     LAQRLELLNLVCFLRKSEGRLFVQINRLPEEKKKMLSRAWIQRILFSVVVAFVMVDGY
                     YRTLGVNSLIYIGDPLDFAVLYTFSLVGILGVHEAGHIVAARWHKIRTSWPYFIPGIP
                     IIGLPTFGALIQSRGLTINRDILFDIAIAGPIAGLVIAIIVSFYGAYTSPVLDSSVAE
                     ELSGDVMRLNDSVFMTLALATYDKAGPDVEVLMSPILFAAWLGFLITFLNLLPAWQLD
                     GGHMARTLFGQKIHRFATYGSVAVLFLLRYEMMAIFILIFSMRNMSARPLDDVSPLSK
                     NRKLLYVVVVVLAVLCAPLPFSVWP"
     gene            4559..5059
                     /gene="rimI"
                     /locus_tag="VGA92_06665"
     CDS             4559..5059
                     /gene="rimI"
                     /locus_tag="VGA92_06665"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX9846132.1"
                     /translation="MQIINRQIGEYIVRRCEPSDLIPVMEINLKTLPEHYSDYFYESL
                     LAEMPEAFLIAERHGKLVGYIMCKTEYGFSNFKKLGFVKKGHVVSVAVLEDYRRQGLG
                     SAIVEEALKGAKEKHCDELYLEVRCSNTDAVRLYEKLGFSIRQRLKSYYRDGEDAFLM
                     AIEFEY"
     gene            5098..5445
                     /locus_tag="VGA92_06670"
     CDS             5098..5445
                     /locus_tag="VGA92_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846133.1"
                     /translation="MEVQKATKRLLDLSPSVRVVTICDMNGKVIFSAHSKRVKNLLSR
                     KESKTSLQSAARSWKVRKSLSRKLGPCKYVLAEYGAVKRIVVPAGKNHLLYVTTTSAY
                     DHNKVIRKARSFR"
     gene            complement(5559..5825)
                     /locus_tag="VGA92_06675"
     CDS             complement(5559..5825)
                     /locus_tag="VGA92_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846134.1"
                     /translation="MRLSEMGIIRIGGRGTTRRVTEKEKDNWAGPAFKEHQQAQKEAS
                     KDKYITMGRGTAKIKFGDIADTPTRSTKGMWVSSGRGTGKRRLD"
     gene            5929..6150
                     /locus_tag="VGA92_06680"
     CDS             5929..6150
                     /locus_tag="VGA92_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9846135.1"
                     /translation="MTRRKATGIFIFCSCIVAFFVYAYLLMLSEWSPIVLHLSMLMMA
                     GGILGVISWIGYMMATTKPSPSSILSDDK"
     gene            complement(6151..6981)
                     /locus_tag="VGA92_06685"
     CDS             complement(6151..6981)
                     /locus_tag="VGA92_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HEX9846136.1"
                     /translation="MLKKALEDALTAQEAAELYSAFDQIGDIIIIRIPDSLLSKKKVI
                     GQTLLETVHPARSVFYQSSDVEGDFRTRNLEVIAGEDNTETEYKEFGCRFKVDVEKAF
                     FSPRLATERNRIAQLVQDGETVINMFGGIGMFSIVAAKKKKCTVYNIDLNPYAARLCE
                     QNVKLNKKLAGQVISIQGDASQVIEDQLQDKGDRTLMLLPERSDEFLASAISATKSGG
                     IIHYYSHQHADKRQDSVELSKQHFLQVCPVKSEILGGRMVRAVGPRYYQTVVDARITK
                     "
     gene            complement(6987..8771)
                     /locus_tag="VGA92_06690"
     CDS             complement(6987..8771)
                     /locus_tag="VGA92_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HEX9846137.1"
                     /translation="MTHRVAVLDKELCQPKKCGLECIKYCPVNKSGADCIILNEEIKK
                     AQIDENLCNGCGICVKVCPFEAITIVNLATELATDKIHQYGMNSFRLYRLPTPKKGEV
                     VGLLGRNGMGKSTVVNILSGNIKPNLGNYTSPPEWDEILKFFAGTELKDHFEKIKNQD
                     IKASIKPQQVYNISQMFDGTAKELLDKYDQRDAASDLIKQLDLGNSVEHDIKELSGGE
                     LQRLAVAVAAVREADFYFFDEPSSYNDVFQRRGVARVIHGLANLGKSVMVVEHDLTLL
                     DFLSDYIEILYGEPAAYGIVSGILSTKVGINVFLDGYIPSENVRFRDKKFSFDVSTSH
                     DDFEKGEVVISYPLLKRNYPAFSVTIEPGQVRKGEVLGIMGANALGKTTLMKMIAGVE
                     KPDEGSIDKAVTISYKPQYIPSDYDVDVVSVLEKANEGPIFGSIEEEQIIDPLKIKKL
                     YNKSIKNLSGGELQKVAVATCLLKKADVYALDEPSAFLDVEDRIAIAKFIQRFVRSFG
                     KSAIIIDHDIQLMDLISDVMVIFEGTSGLEGHATTPLSKSDAMNRFLKSLDITFRRDE
                     HTMRPRVNKAESRLDKQQKNTGHFYYKN"
     gene            8855..8974
                     /gene="rrf"
                     /locus_tag="VGA92_06695"
     rRNA            8855..8974
                     /gene="rrf"
                     /locus_tag="VGA92_06695"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            9053..9256
                     /locus_tag="VGA92_06700"
     CDS             9053..9256
                     /locus_tag="VGA92_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846138.1"
                     /translation="MFTAKIRRIGTSFGIIIPKKVIKEQKLREGDGVQVSILKKKNID
                     DVFTGFGVARGAKPFKRDRTDRY"
     gene            9315..9443
                     /locus_tag="VGA92_06705"
     CDS             9315..9443
                     /locus_tag="VGA92_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846139.1"
                     /translation="MPRCTVCGQELENDIRFLNHMMDKHGFRNPNTGTPDRNTRGY"
     gene            complement(9430..10902)
                     /locus_tag="VGA92_06710"
     CDS             complement(9430..10902)
                     /locus_tag="VGA92_06710"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9846140.1"
                     /translation="MRIRTENTEPAKKKTQALCYFVAEKSDTPFGFSKIDQKLSSTVK
                     QACTETKGKLGSISIVHTHGMIPAQRIVIAGLGPKDKITHETIRLVSGKIAKKSSDLG
                     LDEFCVVSNNLPPGSKIAANAIVEGTKLSLYSFDKYKKEKKSKHPNLTILVSDSAKIS
                     DTIARAQAVSEGVIYTRSIANLPPNECSPAQLAGFAKSLASKNKVKCTVLSKSELKKK
                     GFGGISAVGQGSKNEPKLIIFEYNGKRGEKPTVLVGKAVTFDTGGISIKPADKMDEMK
                     FDKCGGCTVFGIIKAAAELRLPVNLVGIVPSVENMPGGESYRPGDIIKLYSGKTAEIL
                     NTDAEGRLILFDALSYGIKTYKPKEIIDFATLTGACIVALGSNVAGLISNNPRLAEKL
                     KQSSEKTGEEIWQLPLNDDYMDMIKSDYADIKNVGSGRVAGTITAAAFLASAIENTPW
                     AHFDIAGTAWVQGGSKDKSYNPKGATGFGVRLILDYLSSP"
     gene            10986..11153
                     /locus_tag="VGA92_06715"
     CDS             10986..11153
                     /locus_tag="VGA92_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846141.1"
                     /translation="MSSMKCISCGKSFFSPMGYNKCNICAKKDGDSMGHDEHEGHGHE
                     GHDSCGCGHSH"
     gene            11220..11381
                     /locus_tag="VGA92_06720"
     CDS             11220..11381
                     /locus_tag="VGA92_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846142.1"
                     /translation="MSKTQQKTRKSDKLYPKEEAVVKRILSGKEKLYKFDNPEDALGF
                     LRSISKKEN"
     gene            11388..11654
                     /locus_tag="VGA92_06725"
     CDS             11388..11654
                     /locus_tag="VGA92_06725"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9846143.1"
                     /translation="MWKIVYAKTFVHQYQKLDLEIQKRVYNVIRDLQHSEDPAKLGIP
                     KKGQLKNMYAAYVGKYRIIYDIYRKEITIYFVYVGTHKDVYGTD"
     gene            complement(11656..>12063)
                     /locus_tag="VGA92_06730"
     CDS             complement(11656..>12063)
                     /locus_tag="VGA92_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846144.1"
                     /translation="DESEISYHAFEFALDLAKKYHAKLSIITIIHSNILASSFLQVSS
                     HQKIIERQKVSELNHLFKIIESISKRFGVQTYTDIVLSDNVSDSIVSFCSQQKVNLIV
                     MGTRSRTGPRKYLIGSVAVDVLQKASCPVVFVK"
BASE COUNT         3418 a         2557 c         2508 g         3580 t
ORIGIN      
        1 agacggaatg cttgccatgg caataccagt agttcaaaga ccaacatcaa ttatcaagtt
       61 aggaatactt gcaatgactt ggtcttctga acattatagt gtcgatttcg tcaaatctct
      121 tatcaagaag caaggatttg atgttgtatc gctagaaaaa attggctcaa gtgtttatga
      181 gccgctgact gattattatg caaaaaaccg caaatcactc aagccaaaaa ttttaaaaca
      241 atacccatct tatgttgaaa aaatcctctt caattcgctt cacaaaatga aaaaagtctc
      301 agaggacaaa atcattgatt atctactaat agtgtgcaaa aagcaagaag ctaatccaaa
      361 ctaagtgtta ttttaccaag accacggggc aatgagcatt tagcgcaatg ctagttgaga
      421 ctcggccaag catcatttcc tttgcctgcc cctttccatg cgtgcccatt acaatatagt
      481 caacatgttt ttttgtggca aaagagatga ttgcctctgc gatcaaattt gactctaaga
      541 tcaatgactc acacttgaca tggaatctgg atgcctgaaa ctctaacttt ttgaaaattt
      601 ctttgaattc ctcattcctg ctttttccaa cgcctctttg tctgcttggc gtctcattta
      661 cccactgggt tttgtcgctt tcattgctta ctgccactat gactaacatt gatccgtatt
      721 tttttgccaa atcgagtgcg tgtgcaaatg cacgcgatgc atactgcgag ttatcaaatg
      781 gaactagtat gcatttgatt agtgatgcgt taattatctg gcagtcctcc ggtggatttt
      841 tacacactat ttgattctca gccaacggta tcctttgcgc aaacacacct aaaataaccc
      901 agtttgtgcg tattgaaaat accagcacaa aacccgtacg aggcatcagt gaaatcaatt
      961 taagatgtaa gtgactaatc attattggcc agcaatgaag aatcagagtt atatctgaaa
     1021 aatgatgtga aggcatttgt ctgagctgac tacgttttgt tttcataatt ctatatataa
     1081 aaccgatttc taagacccaa gcgatgcctc agacaaaacc catagtcaga attgaaaacg
     1141 tggttgcgtc tgcaacagtt gatcaaaaac tagatctaaa agaaataaca aaaaaatttc
     1201 cagacaccga gtggaaccca gaacagttcc caggacttgt ctttagaatt aaaactccca
     1261 agactgccac tctaattttt tcgtcgggaa aaatggtttg cactggagga aaatccgaag
     1321 acatgacagt aaaggcagtg cgaacagttg tgcagcagct gcgaaaaggt ggaatcaaaa
     1381 taaaaaaaga ggcaatcgta acggtgcaaa acattgtcgc atctgcaaac cttgggggaa
     1441 agatccacct agagaacgca gcgcgagtca tgccaagaag catgtacgag ccagaacagt
     1501 tcccaggact aatccacagg atgcttgacc caaagactgt gatacttttg tttgcgtcag
     1561 gcaagctagt ctgtactggc gcaagaaagg aaagcgacgt gtaccgatcg gttaatgcct
     1621 tgcacaacac gcttgaagaa aaaggcctga tgatttacga tagctaggat ttttccgtct
     1681 gcttttgcac cttatctaga actgattttt caataaggtt gtattcgtgt aggatttttg
     1741 caatctctaa gatgtccagg aggtgatgca gtgtagcgcc ttcttttttt agcgcaccgt
     1801 cagctccctc tagcctgttt actataacgt agacgtctga gatgctcgca ccagcctcct
     1861 ttagggcctt aatgccgttt atgacggagc cgccagtagt tgccacatcg tcgacaacta
     1921 gtatcttcat tccatttttg actattcctt ctaccagctt gcctgttccg tgggccttta
     1981 cctcagacct gacgtatact agaggcttga caatctcaaa ggccaatgaa gaggcaatta
     2041 ctaggccacc tgttggcaca gacgctatgg catcaaagtt atcaaagcct atctgctctg
     2101 atatgaggtt ttgaagatac tttatcatgc gtctgtactg gtgaggatag cttggaacca
     2161 gtcttagatc cacatagtag gagctcttct tgccgcttga tagcgtaaag tctccaaact
     2221 tgattattcc gttttggtgc aaaaacgttg caaactcttt tacaaactcc acaccaaaat
     2281 taactaaact ggatttattt tggtttgtgt tacaggatag tcgtgcctga aatctggtta
     2341 aactatggga caactgacgt tgttcttgat ataaaggccg agaatcttga tcaaaaagtc
     2401 gagtttgaga gccaaactct ggcagactcg gccgtctctg aaaaacttga tgccgtagac
     2461 ctctcaaaac ccctgacatt tgtagtccta aattatactg gctcggtgca aaacgccctc
     2521 tcagtacttt ttgaaaaatg cacacaaaaa ggcgtgccaa ggcccaaagt ttttgcagat
     2581 aggcagataa tgaatttggt aaaggcaaca cttcccccag aaagccaggt gcaagccttt
     2641 gagggaattg aaaacgtaaa cccggacctt gtgtttgtcg gagaaatgga gtttgatggg
     2701 ttgtttggat ttgattcagt ctgcacaaaa attctcagaa gatttggcac agagcaaatg
     2761 ttatcggcat acgaaaaaag gcagggcagt ttgccaaacc ccggtaagga gacgccaagc
     2821 cttgaggttg caaaaaactt tgcaaacgga tttgagatat ctgcaatcga gattgttgcc
     2881 agcccaaaag gaattgtgga tttgtctgtt ggccacccat caaagactct ggaaatttca
     2941 aagacaatgg ggtcctttac taaaacaata gaaaaacacc gaacccttct aatcagcacc
     3001 ggaaaagatg caagcaatga tacgcttggc cattcgcttg gctcgctttg gaactgttat
     3061 tctgccataa gggacgaagg cctggcaatc ttacttgggg agtgcaaggg aggaatcagc
     3121 tcagaagcaa tacggcaata catcgaagga aggatgagca tagagaggct gcaaaagcct
     3181 gcaaaatatg tggatgggat ggaaggtctt ttgtttttat cagagattca aaaaaagata
     3241 aaaattggaa ttgtttcggt tctgccggaa ttctacatca aaaaactgga catgatatta
     3301 tttgatggcg taaaggaaac actagagtat gttctaaagc accagggggc aagacagaaa
     3361 atctcaattg ttcctgacgg tgcgcggatt ctactagggc caaactgaaa acggcagcgg
     3421 tgcacacaaa acggcaagaa caactactac aacatagagc agctttctgt tttttgataa
     3481 tggcgagaca tcatcaagtg gccttgcact catgtttctc atgctaaata tcaggatgaa
     3541 aattgccatc atctcatatc gcaacaaaaa caaaacagca acactaccat atgttgcaaa
     3601 cctgtgaatt ttttggccaa aaagtgttcg tgccatatgg cctccgtcaa gctgccaggc
     3661 aggaagcaaa ttcaaaaacg taattaaaaa gccaagccat gcggcaaaca aaataggtga
     3721 catcaacacc tctacgtctg gtccggcctt gtcgtatgtg gcaagtgcaa gtgtcatgaa
     3781 aacactgtca tttagtctca tcacatctcc tgagagttct tctgcaaccg atgaatccaa
     3841 aaccggagac gtgtatgccc cataaaatga aacaatgatt gcaattacaa gtcctgcaat
     3901 tggccctgca attgcaatat caaataaaat gtcccggtta atagttaagc ctctggactg
     3961 aatcagcgca ccaaaggttg gaagtccaat tattggtatt cccggaataa agtacggcca
     4021 cgaagttctg attttgtgcc atcttgcggc aactatatgc cctgcctcgt gcacacctaa
     4081 aatcccaacc agagaaaatg tatacaatac tgcaaagtca agcggatctc caatgtagat
     4141 taggctgttt acgccaaggg tcctgtaata accgtcaacc atcacaaagg ctacaactac
     4201 actaaaaaga attcgctgaa tccacgcccg cgaaagcatc ttcttttttt cttctggtag
     4261 ccggtttatc tggacgaaca gccttccttc ggattttcgc aaaaagcaga caagatttaa
     4321 caattcaagc ctttgcgcaa gggagacgaa cttttcttta aagtctagat ctgcaacctc
     4381 aaactccatt ttttcaagcg atattatgac gtctctgacc tcaaagactg aggatacaat
     4441 cgagattacc tgttcctgtg ttggctccat tatggatcaa aaaactgcgt tcacattaaa
     4501 tgatttgtat aaaattcaaa actaacatta aatattccag tgtggcgaat ctatatcaat
     4561 gcagataatc aacaggcaga taggcgaata catcgttagg cgttgtgaac caagtgatct
     4621 catcccggta atggagataa acctcaagac gctgcctgag cattattctg attattttta
     4681 tgaaagcctt cttgccgaga tgccagaggc gtttttgata gcagaacgcc acggaaagct
     4741 tgtgggatat attatgtgca agacggaata cgggttttct aactttaaaa aactcggctt
     4801 tgtcaaaaag ggccacgtcg tatctgttgc cgtactagaa gactatagaa ggcagggtct
     4861 aggttccgca atagtcgaag aggcactaaa aggtgcaaaa gaaaaacact gtgatgagct
     4921 gtatttggag gttcgatgca gcaacactga tgcggtaagg ctttatgaaa aactgggatt
     4981 tagcataagg cagaggctca aatcgtacta tcgcgacggc gaagacgcgt ttttaatggc
     5041 aatagaattc gaatactaga ggaaatactt atcaatttta gtgaaattca taatactatg
     5101 gaagttcaaa aggcaacaaa aagacttctt gacctcagtc catcggtgcg agttgttacc
     5161 atttgcgaca tgaatggcaa agtaattttc tctgcacact ctaaacgagt aaaaaacctg
     5221 ctgtcaagaa aggaaagcaa gacttctttg caatctgcag cacgctcatg gaaggtaaga
     5281 aaatcacttt caagaaagct cggaccatgc aaatacgttc tggccgaata tggtgcagta
     5341 aaaaggattg tcgtacctgc gggaaaaaat catctgctct atgtgactac cacgtcggca
     5401 tatgatcaca acaaagtaat tcgaaaggca cgctctttcc gatagatggg caaaccatct
     5461 ctgcaatttt tttaaactct tgataaaata atttagtaga gtactcccgt cttgagagct
     5521 attccattgt atggttctat tgaatctcgg attggaacct agtctagtct tctttttcca
     5581 gttcctctac ctgagcttac ccacatgccc ttagtagatc tggtcggtgt atctgcaata
     5641 tcgccaaact tgatctttgc tgttccgcga cccattgtga tgtatttgtc tttggacgct
     5701 tctttttgcg cttgttggtg ttctttgaat gctggacctg cccagttgtc tttttctttt
     5761 tcagttactc gtcttgtggt tcctcttcct ccgatgcgta ttattcccat ctcgcttaac
     5821 ctcatattca cttgcgtaac gcgtgttaat aaaattttga aaacgtctaa aaaatgccgc
     5881 tttagaacag aatctagaat tatatgactc aagccaaggg tttagctgat gactagacgc
     5941 aaagccactg gaattttcat attttgttcc tgtattgtgg cattttttgt gtatgcgtat
     6001 ttgctgatgc tctctgagtg gagcccaatt gttttgcatc tatccatgtt gatgatggca
     6061 ggcggaattt tgggcgtcat ttcatggatt gggtacatga tggcaacaac aaagccctcg
     6121 ccatcatcca ttctatctga tgacaagtag ctattttgtt attctggcat ctactacggt
     6181 ttggtaatat ctgggaccaa ctgctctgac catacgtcct cccaaaattt ctgattttac
     6241 tggacaaact tgcaaaaaat gctgttttga taattccaca gaatcctgac gcttgtctgc
     6301 atgctggtgc gaataataat ggattattcc gccgcttttt gtagcagata tggcagaagc
     6361 cagaaactca tcggatctct caggtagtag catgagggtt ctgtcgccct tgtcttggag
     6421 ctgatcctct atgacctgtg atgcatcgcc ctgaattgag attacttgtc ctgcaagctt
     6481 tttgttaagc ttgacatttt gctcgcaaag cctggcggca tacgggttta ggtcgatgtt
     6541 gtaaacggtg cattttttct tttttgcggc aacaattgaa aacattccaa tacctccaaa
     6601 catgttgatg actgtttcac cgtcttggac aagttgtgca attctgtttc gctcagttgc
     6661 aagccttggc gagaaaaacg ccttttctac gtcaactttg aatctgcatc cgaactcttt
     6721 gtattctgtt tcggtgttgt cctctcctgc gatgacttca agattccttg tcctaaaatc
     6781 gccttcgaca tcggaggact ggtaaaacac agagcgcgca gggtgaacgg tttcaagcag
     6841 ggtctggcca attactttct ttttggataa caacgagtct ggaattctga taattatgat
     6901 atcaccaatc tgatcaaacg cagaatacag ctctgcggct tcctgggcgg taagcgcgtc
     6961 ctccagtgct ttttttagca tccgagtcaa ttcttgtagt aaaagtgacc tgtgtttttt
     7021 tgctgcttgt ccagcctgct ttctgccttg tttacgcggg gcctcatagt gtgctcgtcc
     7081 cgcctaaatg tgatgtctag ggattttaga aacctgttca ttgcgtctga ctttgagagt
     7141 ggtgttgtag cgtgtccttc aagtccggat gttccttcaa aaattaccat cacgtctgag
     7201 atcaaatcca ttaactgtat gtcgtggtca attatgattg cggattttcc aaaagagcgc
     7261 acaaaccttt gaatgaattt tgcaatcgcg atcctgtcct ccacatccaa aaacgccgaa
     7321 ggctcgtcta gtgcgtatac gtctgctttt tttagaaggc atgttgccac tgccaccttt
     7381 tgcaattctc cgccagagag atttttaatt gacttgttgt agagtttttt gatttttagt
     7441 gggtcgatta tttgttcttc ttcaatgctt ccaaaaattg ggccctcgtt tgccttttct
     7501 agtaccgata cgacatctac gtcatagtca cttgggatgt actgcggctt gtaggaaatc
     7561 gtgactgcct tatcgatgct tccctcgtct ggcttttcta ctccggcaat catcttcatc
     7621 aacgttgtct tgcctagtgc gtttgcaccc atgattccaa gtacctctcc ctttcttacc
     7681 tgcccaggct ctattgtcac agaaaatgct ggatagttgc gttttagcaa tggataagaa
     7741 attacgactt cgcctttttc aaaatcatca tgtgatgtag atacatcaaa gctgaacttt
     7801 ttgtccctga accggacgtt ttcacttggg atgtatccat ccaaaaagac attgattcca
     7861 acctttgttg aaagaattcc tgagactatg ccgtatgctg ccggctcgcc gtacaatatc
     7921 tcgatgtaat cggaaagaaa atccaaaagt gtcaggtcgt gctcaactac cattactgac
     7981 ttgcccaaat tggcaaggcc gtggattacc cgtgccacac cccttctctg aaacacgtca
     8041 ttatatgatg acggctcatc aaaaaagtaa aaatcagcct ctcttactgc agctactgca
     8101 actgccagcc tctgaagctc gccaccactc agctccttta tgtcatgctc tacagagttt
     8161 cccaagtcaa gttgcttgat gagatcagag gcagcatccc tttggtcata tttgtcgagc
     8221 agctcctttg ctgttccatc aaacatctgg gaaatgttgt agacttgttg gggcttgatt
     8281 gaggccttga tgtcttggtt ttttattttc tcaaaatgat cttttagctc agttcccgca
     8341 aaaaatttta aaatttcatc ccactctggg gggcttgtat aattgccgag atttggcttt
     8401 atgttcccag acaaaatatt gactacggta ctttttccca ttccgtttct tccaaggagt
     8461 ccgacgactt cgcctttttt tggagtgggg agacgataca gcctaaacga gttcatcccg
     8521 tattggtgga ttttgtcagt tgcaagctct gttgccaaat ttacaattgt gattgcctca
     8581 aaggggcaga ctttgacgca tattccacag ccgttgcaga ggttttcgtc tatttgcgcc
     8641 ttttttatct cctcatttag tatgatgcag tccgctccgg acttgtttac cggacagtac
     8701 ttgatgcatt ctaggccgca cttttttggc tggcataact ccttgtctag tactgcgact
     8761 ctatgtgtca tgacggtata cttgatttgt tcaatttata ttcaatccgt atgcgagtcg
     8821 taatcttaat acagaacatt tattgaaatt tcttcaagcc ggccacagag ttggggcgcg
     8881 acccagtctc attccgaacc tggaagtcaa acccaacatc gctactgtgc tactaagatg
     8941 cgagagctct tgggaagcag tagtgctggc atcttttaat aatttgaggc caaaacctct
     9001 ggattttggc aaaaaatgtt tttaggcgtt gtaatgcatt atgatataca atttgtttac
     9061 agctaagata agaagaatcg ggacatcttt tggcatcata attccaaaga aagtgattaa
     9121 ggaacaaaaa ctccgagaag gcgatggggt tcaagttagc atattgaaga aaaaaaacat
     9181 tgatgatgta ttcacgggat ttggcgtcgc acgcggagca aaaccattca aacgagatag
     9241 aacagataga tattgatgtt attgtgattt tagatcacaa ataatgatat aacaatgtaa
     9301 ttttcagatc attaatgcca cgatgtactg tttgcggaca agaactagaa aacgacatta
     9361 ggttcctaaa ccacatgatg gataaacacg gatttagaaa cccaaacact ggcacaccgg
     9421 acagaaacac taggggctac taagataatc cagaatcaac cttacaccaa atcctgttgc
     9481 accctttggg ttgtaggatt tgtctttgga gccgccctga acccatgcgg ttcctgcaat
     9541 atcaaagtgg gcccacgggg tgttttctat ggcacttgcc aaaaacgcag cagcagtaat
     9601 ggtgccggca actcgacctg agccaacatt tttgatatct gcataatctg actttatcat
     9661 gtccatgtag tcatcgttta gtggtagctg ccagatttcc tcgccggttt tttctgagga
     9721 ttgttttagc ttttctgcaa gtcttggatt attgcttatc aaccccgcta catttgatcc
     9781 gagggcgaca atgcatgcgc cagtaagtgt ggcaaaatca attatctctt ttggcttgta
     9841 tgtcttgatg ccatatgata acgcatcaaa gagaatcaat cttccttctg catctgtgtt
     9901 tagaatttct gcagtttttc cgctgtatag cttaatgatg tcgcctggtc tatacgactc
     9961 gccacccggc atgttctcaa cagatggtac gattccaacc agatttactg ggagccttag
    10021 ctccgctgca gccttgatta ttccaaaaac ggtgcagccg ccgcacttgt caaacttcat
    10081 ctcatccatt ttgtctgcag gtttgattga aattccgcca gtatcaaatg tgactgcctt
    10141 tcctactagt acggttggct tctctcctct ttttccattg tattcaaaaa taatcagctt
    10201 tggctcgttt ttgcttccct gtcctactgc ggaaattccc ccaaatcctt ttttctttag
    10261 ctcagatttt gacaatactg tacattttac cttgttttta gatgcaagcg attttgcaaa
    10321 gcctgcaagt tgtgctggac tgcattcgtt tggaggcaaa ttagcaatgc ttcgtgtgta
    10381 gatgactcct tctgagacag cctgtgccct tgctatggtg tctgaaattt ttgcagaatc
    10441 tgagactaaa attgtgaggt ttggatgctt tgattttttt tcttttttgt atttgtcaaa
    10501 agagtatagt gaaagctttg ttccttccac aattgcgttt gcagcaattt tggagcctgg
    10561 cggcaaatta tttgaaacaa cacaaaactc gtctaaccca agatcgcttg atttttttgc
    10621 gattttgcct gagactagcc ttattgtttc atgggtgatt ttgtcctttg gtccaaggcc
    10681 ggctattacg attctttggg ctggaatcat accatgggtg tgaacaatgg agatactgcc
    10741 aagttttcct ttggtttccg tgcatgcctg ctttactgtt gaagataatt tctgatcaat
    10801 ttttgaaaac ccaaacgggg tgtctgattt ttctgcaaca aaataacata atgcctgagt
    10861 ttttttcttt gcaggctcgg tgttttctgt tctgattctc acaaactgac tacatttttt
    10921 tggatattat agctttatcg aaaataaagc gtatcgataa atagtggcac cttcaacgaa
    10981 gctatatgag tagtatgaaa tgcatctcgt gcggaaaaag cttcttctcg cctatggggt
    11041 acaacaagtg caacatttgc gccaaaaagg acggagacag catgggtcat gacgaacatg
    11101 aaggacatgg acacgaagga catgattcgt gtggttgcgg acactcccac taaattttta
    11161 atttagtcat cgtacaaatc gacaaatcct taattataaa gaaagagtaa gaagagtaat
    11221 tgtctaaaac acaacaaaaa acgcgtaagt cggataaact atatccaaaa gaggaggcag
    11281 tagttaaaag gatactttct ggcaaagaga agctctacaa atttgacaat ccagaagatg
    11341 cccttggatt tctacgttcc atttctaaaa aagaaaactg agataatttg tggaagattg
    11401 tatatgcaaa aacctttgtt catcaatatc aaaaactaga tttagaaata caaaaacgtg
    11461 tgtacaacgt aatcagagac ctccaacaca gtgaggatcc ggcaaagctt ggcataccaa
    11521 agaaaggtca attaaaaaat atgtatgctg cttatgttgg aaaatacaga ataatttatg
    11581 acatttacag aaaagaaata actatctatt ttgtatatgt cggtacacac aaggatgtct
    11641 acggaacaga ttgaatcatt ttacaaaaac gactgggcag cttgcctttt gaagtacgtc
    11701 aactgcgaca ctgccaatta ggtattttct tggacctgta cgggatctag ttcccataac
    11761 tatgaggttg actttttgct ggctgcaaaa cgacacaatt gaatcagata cgttgtcgga
    11821 aagcacaatg tcggtgtatg tctgcacgcc aaaccttttt gaaatggact ctattatttt
    11881 gaataaatga tttagctcag agactttttg tcgctcaatt atcttttgat ggctgcttac
    11941 ttggagaaac gagcttgcca aaatgttgct gtgtattatg gtgatgatgg aaagttttgc
    12001 gtggtatttt tttgccaaat caagcgcaaa ctcgaatgca tgatacgaga tctctgactc
    12061 gtc
//
LOCUS       DASWKH010000085         7209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_215893, whole genome shotgun sequence.
ACCESSION   DASWKH010000085 DASWKH010000000
VERSION     DASWKH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7209
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215893"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGA92_06735"
     CDS             <1..501
                     /locus_tag="VGA92_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9846145.1"
                     /translation="IYGIQYDIMQIDPYPSIADLFFAALYIFWIIHIITNIRYFGTTL
                     EHKKLIVFIMIFCAVTFSYIGITLYQLHLEPNFEFFYGLLFVVLSGITLPFVVYTAMI
                     FKDSALGKAWYVLLLAFLINISGDVWYYYLEVFEQYDLHHPVNFFWYIGYWIQTYALY
                     KHKKVT"
     gene            586..1251
                     /locus_tag="VGA92_06740"
     CDS             586..1251
                     /locus_tag="VGA92_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX9846146.1"
                     /translation="MSDRMFFNFSFFKIDPKWRWMADLAKEESAKEVEQVLRNSKIKC
                     RTYSTLGLRDDAEFLLWFASESIEEIQGIISKIYTTVFGKYITPSHVYLSSTRPSIYA
                     KSGKTPSFVDGAEPKNYVIVYPFIKTREWYLLPLEQRKVMMDEHITIGHKYPQVTLNT
                     TYSFGIHDEDFMLAFETDDLHAFQDLIMELRETKVSRYIEKDTPMIVCVKKDIVSLIS
                     SLG"
     gene            complement(1269..1676)
                     /locus_tag="VGA92_06745"
     CDS             complement(1269..1676)
                     /locus_tag="VGA92_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9846147.1"
                     /translation="MKDRQTRLYDTSAFLNLLLNEGSKSLSVLSGQAVLDLTTYELGN
                     SIWRLSYLQKKITKAEACSLLDSCLKVISSMNVLHIKGLEEDVKELSSGMGQSFYDSA
                     YLVVAKNHNLELVTDNKKLLKAALNSKIKASPS"
     gene            complement(1666..1893)
                     /locus_tag="VGA92_06750"
     CDS             complement(1666..1893)
                     /locus_tag="VGA92_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846148.1"
                     /translation="MSKVVSAKVPDELKKKADKYGIKVGALLRESLEAKINAIERQLL
                     SSKLDDLSSKVGSKIKKQDIIKAVRSSRDER"
     gene            complement(2017..2895)
                     /locus_tag="VGA92_06755"
     CDS             complement(2017..2895)
                     /locus_tag="VGA92_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX9846149.1"
                     /translation="MVSIRKKVKGGRTYYYLKHNAGTFQREAYLGTQIPKNIVHLKRQ
                     FLIDLQRERWSPSIDAIKKGYSENLKKTPNSILEQNLIEFSYYFTHDTQKIEGSSLTQ
                     QETYNLLRFHITPDKKPESDMIEAQRHHDVFLKIMNSKRDISLKYVLSWHSELFERTK
                     PEFAGKVRHFPIWVTGSRSTFPHARFVPAFLKDFFSWYEKSKAKIHPVELAGLAHFRF
                     VNIHPFGDGNGRVSRLIMNKILHQCGFPLLNIRYGERKFYYVALEKGNLKSEELYFLK
                     WFVKKYISENKRYAKM"
     gene            3029..3574
                     /locus_tag="VGA92_06760"
     CDS             3029..3574
                     /locus_tag="VGA92_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HEX9846150.1"
                     /translation="MHALKEWATIVSALESGDQTVLLRKGGILEVSSGFVVESKKFLL
                     FPTYEHQSSDNIKPQFQKHLDSVMKNKPQDTQNRITSYAEVVSEADLTDQKKIQQISE
                     FHIWSDSYIQTRLNWMPQKAIKAMFLKAYKMPEFEIASKPEFQGCKSWIDINAKPDSA
                     QAVLSDSELRLRLEKFEEIIS"
     gene            3571..4569
                     /locus_tag="VGA92_06765"
     CDS             3571..4569
                     /locus_tag="VGA92_06765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9846151.1"
                     /translation="MRYKKLGKTDITVSEIGFGAWTIALDWWGKKIEEDEAKKMIKKA
                     YDLGINFFETADMYGKGKSEKILGEALKGMRNEVVISTKYGYDFGGVDQIGHSELPQR
                     FDPLFTDHALKNSLARLQTDHIDVYGLHNPKLAHIRDDTIFYTLDSKINDGLIGTYQV
                     ALGPAIGWTQEGLESMNRKNVSAVQTVYNILEQTPGNELIRNAEQKNVGILVRVPDAS
                     GILTGKVNAETKIDQKDHRSVRKGEWIKSALDKVEQLRPIANRNGLSITELAIKFILS
                     KNAVASVLPTVISEQEVEQFAQISDGKYLPESDMSEISRLYDSWPLYELKATVQAA"
     gene            4646..5026
                     /locus_tag="VGA92_06770"
     CDS             4646..5026
                     /locus_tag="VGA92_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9846152.1"
                     /translation="MYEIPTAIVVDDSKMTVQVLCDYLAMIDVDVLGRGHNGKDAVEL
                     YKKYRPDIVFLDIMMPDYDGYYALENIKKMDTNAKVVVVTSDLRQKDSKELERLGPTK
                     IIFKPFAIDTIKDIVEKIRKSKQK"
     gene            5091..5474
                     /locus_tag="VGA92_06775"
     CDS             5091..5474
                     /locus_tag="VGA92_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846153.1"
                     /translation="MPRQQVLYYAAAGTTAIAGIIHLILALDEPKFGLNSDTLFLVGG
                     AAQIFWIVPMIKKWGTPWYIIGISGTAAFVAIWTITRMQDNPITGRAGSVNQNGIIVE
                     AMQLAFIGLVISILAYEKRRITNVK"
     gene            complement(5554..>7209)
                     /locus_tag="VGA92_06780"
     CDS             complement(5554..>7209)
                     /locus_tag="VGA92_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HEX9846154.1"
                     /translation="TIPDDVALGSGSLSYPSSVSTYLISSQDTLTIDAGDTLTIPSGK
                     LITNSGTLTNSGTIANAGTLTLTGTAFPGGVITNSGIMTNNGAINIDRFSRITNSGTI
                     DNFDTIENSGIITNLGTLANSGTVANLGTINNSNTITNSNAIENSGTIANAGFITITS
                     SGTLTNSGFIGNLNFARITNSGTIDNFDTITNSGTIENLGIITNQCDATYSGNPPSGN
                     AVDNISCDTSPPVIVILGDNPATHEAVTPYVDAGATALDDVDGDLTSAIIVTNNVNEN
                     TPGPYTVEYSVTDSSGNTASAQRTVNVMDTMPPAVTISENITAEATGPDGATVSYDFP
                     TATDAVDDSPTVQCTPASGSVFALGDTTVSCIATDFSGNSADTSFTVTVQDTTPPEIT
                     ILGENPATILENLAYADAGATASDIVDGDLTASIITDNPVDISVPDTYTVTYTVSDNA
                     GNTSVAERTVIVKPIEEATDDLGDQVGSLGLPKGAATSLASNLDSAISILSDNNPNND
                     SAACGMLGSFVNKVQAQSGKTIDSADADSLIADAELIRTALGC"
BASE COUNT         2049 a         1467 c         1639 g         2054 t
ORIGIN      
        1 atctatggta tccagtatga catcatgcaa attgatccat acccatctat tgccgatttg
       61 ttttttgctg cactttatat tttttggata atacacatca ttacaaatat cagatatttt
      121 ggaaccacac ttgaacataa aaaactaatc gttttcatca tgattttctg tgctgtcaca
      181 ttttcatata ttggcatcac gctttatcag ttacaccttg aaccaaattt tgaattcttt
      241 tatggtttgt tgtttgtagt cttatcagga atcacattac ctttcgtagt ttacacggca
      301 atgattttca aagacagtgc gcttggtaag gcatggtatg ttttgctgct cgcgtttttg
      361 atcaacattt caggtgatgt atggtattat tatctcgaag tctttgagca atatgatctg
      421 catcatccag ttaatttctt ttggtatata ggatactgga tccagacata cgcattgtac
      481 aaacataaaa aagtcacata atcacatatg caggtttcat tattggatac aaaccccaac
      541 ttttgaacag aacattgtta aatatagtca cgcggaaatc aaaccatgtc agataggatg
      601 tttttcaact tttccttctt caaaatagac ccaaagtggc gctggatggc agacctagca
      661 aaagaagaat cggcaaagga agtagagcag gtgctaagaa actccaagat aaaatgcagg
      721 acatactcga ctcttggtct tcgggatgac gcagaatttc tgctctggtt tgcatcagag
      781 tcaatagagg aaatacaggg aataatctca aaaatctaca cgacagtctt tggaaaatac
      841 atcacgccgt cacatgtcta cctctcatct actaggccgt caatttatgc aaagtcaggc
      901 aaaaccccgt cctttgtgga tggcgcagag ccaaaaaact acgtcatagt atacccgttt
      961 atcaagaccc gcgagtggta tctacttccg cttgagcaaa gaaaggtaat gatggatgag
     1021 cacatcacaa taggccacaa gtacccacag gtgacactaa acaccacgta ctcgtttggc
     1081 attcacgacg aggactttat gcttgccttt gaaaccgatg atctgcatgc gttccaagac
     1141 ttgataatgg agctgcgcga aaccaaggta tctcgataca tagaaaagga cacgccaatg
     1201 attgtctgcg tcaagaagga tatcgtatca ttgatttcta gtcttggcta gttttcaaat
     1261 gatcattctt atgaaggaga cgccttgatc ttagaattta atgcggcctt tagtagcttt
     1321 ttgttgtctg ttaccagctc aaggttatga ttttttgcga caaccaggta tgcagagtca
     1381 taaaacgact ggcccatacc tgacgatagt tccttgacat cctcttctaa tccttttatg
     1441 tgtagaacat tcatgctgga aataacttta agacaggaat ccaaaaggga gcaggcttca
     1501 gcctttgtga tctttttttg caaatacgat aacctccata tgctgttgcc aagctcgtac
     1561 gtcgtaaggt caagcactgc ctgaccggac aaaacagaaa gtgattttga gccctcattc
     1621 aaaagcagat tcagaaaggc actggtatca tagagtcttg tttgcctatc tttcatctct
     1681 ggatgaccta acggctttga taatgtcttg cttttttatc ttggagccaa ccttgctgct
     1741 aagatcatca agcttcgatg agagcagctg acgctcgatt gcgttaattt ttgcctcaag
     1801 agactccctt agaagcgccc cgactttaat tccgtacttg tctgccttct tttttagctc
     1861 atctgggacc ttggctgaaa ccactttgct catagtaata ttatatgagt attaccttaa
     1921 aagtattacc aatatggtag actactttaa gatgcaccga gtatcgtctg cgtcaagaag
     1981 gatattattt cgctggttgc aagccttgga taattttcac atctttgcat atcgtttgtt
     2041 ttctgagata tattttttta caaaccattt tagaaagtac agctcttctg attttagatt
     2101 gcctttttcc agcgccacat agtaaaattt ccgttccccg taccttatgt tcaagagtgg
     2161 aaagccgcac tggtgaagaa ttttgttcat gattaatctt gataccctac cgttgccatc
     2221 gccaaaggga tggatattta caaatctaaa gtgggcaagc cctgcaagct cgactgggtg
     2281 tatttttgcc ttggattttt cgtaccaaga aaaaaaatcc ttcaaaaacg caggaacaaa
     2341 tctagcgtga ggaaatgttg atctgctacc tgttacccag attgggaaat ggcgtacctt
     2401 tccagcaaat tccggcttgg ttctctcaaa tagctcagag tgccaactca ggacatattt
     2461 tagtgagata tcccttttgc tattcataat ttttaaaaat acatcgtggt gtctttgcgc
     2521 ctcgatcata tcagattctg gttttttgtc aggcgttatg tgaaacctaa gaaggttgta
     2581 tgtttcttgt tgggtaagtg acgatccctc gatcttttgc gtatcgtgtg taaaataata
     2641 ggaaaactcg attagattct gttctagaat tgaatttggt gtttttttga ggttttcaga
     2701 atagcctttt tttattgcat caattgatgg gctccacctt tccctttgta aatctatgag
     2761 aaactgacgc ttgagatgca ctatgttttt tgggatttgt gtacccaaat aggcttcacg
     2821 ttgaaatgtg ccggcattgt gtttgaggta ataataggtc ctccctccct ttaccttctt
     2881 tctgattgaa accattatta gttattgccc cttgtaatta ttaaaatttg ttaatttttg
     2941 taaatttcgg tgggtacaac atagaaagac atgccgatga gcgtgtgtca agaaggatat
     3001 tatttcgctg gttgcaagtc ttgggtagat gcatgcgcta aaagaatggg caacaatagt
     3061 atcagcttta gagtcaggag accagaccgt attgctgcgc aaaggcggaa tactggaagt
     3121 gtcatccgga tttgtagtcg agtcaaaaaa gttcctcctt ttcccgacat atgagcacca
     3181 aagttctgat aatatcaaac cgcagttcca aaaacacctg gattccgtga tgaaaaacaa
     3241 accccaagac actcaaaaca ggattacctc gtatgccgag gtagtctcag aagcagacct
     3301 cacagaccaa aaaaagattc aacagatatc agagtttcac atctggtcgg actcgtacat
     3361 acagaccaga ctaaactgga tgccacaaaa agcaatcaag gccatgttcc tcaaggcata
     3421 caaaatgccg gaatttgaaa tcgcgtcaaa accagagttt cagggctgca aatcttggat
     3481 agacataaat gcaaaaccag attctgcaca ggcagtgttg agtgattctg aactgagatt
     3541 acgactcgaa aaatttgagg agatcatatc atgaggtaca aaaaacttgg gaaaaccgac
     3601 attacagtat cagagatagg gtttggtgca tggacaatag cgctggactg gtggggcaaa
     3661 aaaattgagg aagacgaggc aaaaaaaatg ataaaaaaag cctacgactt ggggataaac
     3721 ttctttgaga cagcagacat gtacggcaag ggaaaatcag aaaaaattct aggcgaggcc
     3781 ctcaagggga tgcgcaacga agtggtaatt tctacaaagt acggttatga ttttggagga
     3841 gtggatcaga tagggcattc tgagctgccc cagcggtttg acccattatt tactgatcat
     3901 gcgctaaaaa acagccttgc aaggctccag accgaccaca ttgacgtgta cggcctgcac
     3961 aacccaaagc ttgcccacat acgggatgac accatatttt acacacttga ttccaaaatt
     4021 aacgacggac taatcggcac ataccaagtt gcactcggtc ctgcgattgg gtggacgcag
     4081 gagggcctcg agtcaatgaa tcgaaaaaac gtctctgcag tccagaccgt ctacaacatc
     4141 ctagagcaga cgcccggaaa cgagttgata cgaaatgccg agcaaaaaaa tgtcggaata
     4201 ctggtccggg ttccagacgc atccggaatc ctgactggca aggtaaacgc cgagaccaag
     4261 atagaccaaa aagaccaccg ctctgtgcgc aaaggcgagt ggataaaatc cgcactagac
     4321 aaagtagaac agctcaggcc cattgcaaac agaaatggcc tttcgattac agagcttgca
     4381 atcaagttca ttttatcaaa aaatgcggtg gcatcagttc tgcctaccgt aatatccgaa
     4441 caagaagtcg agcagtttgc gcaaatctcc gatggaaaat atctgcccga gtctgatatg
     4501 tcggagatat caaggttgta tgattcgtgg ccactgtacg agctcaaggc aactgtacaa
     4561 gctgcataat atcccaagca atgtaattta gatagttgta ggaaaaacgt ttattggttg
     4621 tgagtgggaa gtatcttgag gcgccatgta cgaaattcct actgcaatcg tagtcgacga
     4681 cagcaagatg acagtacagg tattatgcga ctacctggca atgatcgacg ttgacgtgct
     4741 tgggcgtggc cataacggta aggacgcagt ggaactatac aaaaaataca ggccagacat
     4801 tgtgtttttg gacataatga tgcccgacta cgacgggtat tacgcgctag aaaacatcaa
     4861 aaaaatggac acaaacgcaa aggtagtcgt agttacatca gacctgcgcc aaaaggactc
     4921 aaaggagctt gagcgactag ggcccacaaa aatcatcttc aagccatttg ccatagatac
     4981 aatcaaggac atagttgaaa aaattagaaa atcaaaacaa aaatgattta ctcaaatcaa
     5041 gtaacaatgt gacgtaaatc ttaagtttga atgtcagaga cgataaaaag atgccaaggc
     5101 agcaggtctt gtattatgca gcagctggaa ctactgccat agccggcata atacatctaa
     5161 tacttgctct agacgagcca aaatttggcc taaacagcga tacgctgttt ttagtcggag
     5221 gggcagctca gatattctgg attgtgccaa tgatcaaaaa atggggaacg ccgtggtaca
     5281 ttatcggaat cagtggaact gcagcctttg ttgcaatatg gacaataact cgaatgcaag
     5341 acaacccaat taccggtagg gccggctctg taaaccaaaa cggaatcata gttgaggcaa
     5401 tgcagcttgc attcatcggc cttgtgatat caattcttgc gtatgagaaa agaagaatca
     5461 ctaatgtaaa ataatttgaa aactgttttt tgtgttttta aaaggtttcc attgtatgcg
     5521 gatcgttatt taatgagcaa ttgatttttc tagttagcag cctagcgccg ttcttatcag
     5581 ttctgcatct gcaattaacg agtctgcgtc agcagagtca atcgtcttgc cgctttgagc
     5641 ctgcaccttg tttacaaaag aaccaagcat gccgcatgct gcagaatcgt tgtttgggtt
     5701 gttgtcactt aggattgata ttgcgctgtc aaggttgctt gcaaggcttg ttgcagctcc
     5761 ctttggcagg ccaagagaac ctacctgatc acccagatca tcggttgcct cttctattgg
     5821 ctttactata actgtccttt ctgccactga tgtgttgccg gcattatcgg ataccgtata
     5881 agtcacagtg tacgtgtctg ggaccgatat gtcaactggg ttgtctgtaa tgatgcttgc
     5941 tgtcaggtcg ccatccacaa tgtcagaggc agttgctcct gcgtcggcgt atgctagatt
     6001 ctcaagtatg gttgctggat tttctcccag tatggtaatc tccggaggtg ttgtgtcctg
     6061 tacggttact gtaaatgatg tatcagcaga gtttccagaa aagtcagtag cgatgcagct
     6121 taccgtggtg tcgccaagtg caaatacaga acctgatgca ggagtacact gtactgttgg
     6181 ggaatcgtcg actgcgtccg ttgctgttgg gaagtcatag gaaacggttg ctccgtctgg
     6241 ccccgttgct tcagctgtga tgttctcaga aatagttact gccggcggca ttgtgtccat
     6301 cacatttact gtcctttggg ccgatgcagt atttccagac gagtcagtca ccgagtattc
     6361 tacggtgtac ggtcctggcg tattttcatt tacgttattt gttacgatta tagctgatgt
     6421 aaggtcgccg tctacatcat caagtgcggt tgctccagca tcaacatatg gtgttactgc
     6481 ctcatgagtt gcagggttgt cacctagaat gacaattact gggggggagg tatcgcatga
     6541 gatgttgtcg acggcatttc cactaggtgg atttccactg tatgttgcat cacattgatt
     6601 ggtgattata cccaggtttt ctatagtgcc ggagttggtt atggtgtcaa agttatctat
     6661 ggtgccggag ttggtgattc tggcgaaatt taagttgccg ataaagccgg agttggtgag
     6721 agtgccggag cttgtgatgg taatgaagcc ggcgttggct atagtaccgg agttttctat
     6781 tgcgtttgag ttggtgatgg tatttgaatt gttgatcgta cccaggttgg ccacagtgcc
     6841 ggagttggct aacgtaccaa ggttggtgat gatgccggag ttttctatgg tgtcaaagtt
     6901 atctatggtg ccggagttgg tgatcctgct gaatcgatct atatttatgg caccattatt
     6961 tgtcatgatg ccggagttgg tgatgacacc gccggggaat gcagtaccag taagagttag
     7021 ggtgccggcg ttggctatag tgccggagtt ggtgagagtg ccggagttgg tgattaattt
     7081 gccggacggt atggttaacg tatccccagc atcaatggta agcgtatctt gagaggatat
     7141 caaatatgtg ctgactgagc ttggatatga caacgagcca gaacccaatg cgacatcatc
     7201 tggaatggt
//
LOCUS       DASWKH010000086         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1324255, whole genome shotgun sequence.
ACCESSION   DASWKH010000086 DASWKH010000000
VERSION     DASWKH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324255"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /gene="trxB"
                     /locus_tag="VGA92_06785"
     CDS             complement(<1..933)
                     /gene="trxB"
                     /locus_tag="VGA92_06785"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9846155.1"
                     /translation="MMAATSENTIQVEKNPPKKPDKKKTKYDVIIIGAGPAGYTAGIY
                     CARARHDTLILSGILPGGQLVNTTDVENYPGFAEGIMGPDLMIVMRKQTEKMGATIID
                     DEAVDVDFRRKPFKVLTASEEYEAKAVIICTGADYRKLGLEGEKTFAGKGVSYCATCD
                     GAFFRNQELIVVGGGDSAMEESTFLTKFASTVHVVHRKDTLRASKIMQERAMANPKIK
                     FHWNKEIEAISGNQKVQSVTLKDTKTGEKTTLNVEGVFVAIGHVPSSKIFQNQIELDK
                     NGYVVLKNNTETSVKGVFAAGDVHDHTYKQAVTAA"
     gene            complement(1422..>1746)
                     /locus_tag="VGA92_06790"
     CDS             complement(1422..>1746)
                     /locus_tag="VGA92_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846156.1"
                     /translation="SLQLLREGNLRSVAGHLGLDQSIPADGSVCVFFMSDLNEVLDKF
                     GNRGYRASQLEASTIGGKFYLTSYAQGLGATGLTFYDDEVTQFFSPHAKNKSVMFLVA
                     IGKKA"
BASE COUNT          478 a          366 c          323 g          579 t
ORIGIN      
        1 tgcagcagta acagcctgtt tgtatgtgtg atcatggaca tcacctgcag caaaaactcc
       61 ctttacgcta gtctctgtgt tattttttaa tacaacatat ccatttttgt caagctcgat
      121 ttggttttgg aaaatctttg aacttggcac atggccgatt gcgacaaaaa caccttcaac
      181 atttaatgtg gttttttcac cagtttttgt atcctttaat gtgacacttt gtactttttg
      241 gtttcctgag attgcctcaa tttccttgtt ccagtgaaat tttatttttg ggtttgccat
      301 ggctcgctcc tgcatgattt tgcttgccct tagagtgtct tttctgtgaa ccacatggac
      361 agttgaggca aattttgtaa gaaaggtgga ttcctccatt gcggagtcgc ctccgccgac
      421 aacaattagt tcttggtttc taaaaaaagc accatcacat gttgcacagt atgatactcc
      481 ctttcctgca aaggtctttt cgccttcaag gccaagtttt ctatagtctg cacccgtgca
      541 gataattact gctttagctt cgtattcttc tgatgcagta agtaccttga atggcttgcg
      601 cctaaaatca acgtctactg cctcatcatc aataattgtt gcacccattt tctctgtctg
      661 ctttctcata actatcataa ggtcaggtcc catgattcct tctgcaaagc ctggatagtt
      721 ttcaacgtct gttgtgttta ctagctgacc tccaggcaag atgcctgata agattagagt
      781 gtcatgtctt gctcttgcgc agtaaatgcc tgctgtatat cctgcaggtc ctgcgccgat
      841 aattattacg tcgtattttg tctttttttt gtctggtttt tttggtgggt ttttttcaac
      901 ttgtatggta ttttctgaag tagctgccat catagagtaa aaccgattaa ttcaatatta
      961 aagtccatct ttgatatggc tgtgttgaat ctcaagtttt tgtaagatag gacagttttg
     1021 tatcaagatt atagaaaaat tagcaggatc gaaaagccag taaccaacaa tactaaatgc
     1081 caagaaaatc tactctagta aatcttgatt aatctaggaa aactctttct acaaatacgc
     1141 tatgattgca gctcacaaga taaggaaaat cttgaagagg tgcaaaatca aattcaagag
     1201 agaatcaatt ttctcaatga acagaatctg gacagctacg gtgatcccac gcttttagag
     1261 tactacgaac tatacagtca gattcagcaa tgcatttttc aggaccatac aggtcaatac
     1321 gtgctcatag caagcatcat tgcagggtta tcttttcttg tttactttcg gtggtggatg
     1381 ataaagaaag gaggaactaa aaagaaatac taaattaaac ttcatgcctt tttacctatt
     1441 gcaaccaaaa acattacact tttgtttttt gcatgtggtg aaaaaaattg agttacctca
     1501 tcatcgtaaa aggtaagacc tgttgcaccc agcccctgtg cgtaagaagt aagataaaac
     1561 tttcctccga ttgtgcttgc ttccagttgc gatgccctgt atccacgatt tccaaatttg
     1621 tcaagaacct cattcaaatc actcataaag aaaacacata cactcccatc tgcaggaatg
     1681 ctctgatcca gccccaagtg gcctgcaacg cttcttaggt ttccctctct gagtagttgg
     1741 agtgag
//
LOCUS       DASWKH010000087         5441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_601697, whole genome shotgun sequence.
ACCESSION   DASWKH010000087 DASWKH010000000
VERSION     DASWKH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601697"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..393)
                     /locus_tag="VGA92_06795"
     CDS             complement(223..393)
                     /locus_tag="VGA92_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846157.1"
                     /translation="MVLIVPNEDFISFSWKFIREKHLSADGAHGDYNNDTPTAVTCAL
                     ALLDLQANMGDG"
     gene            490..996
                     /locus_tag="VGA92_06800"
     CDS             490..996
                     /locus_tag="VGA92_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846158.1"
                     /translation="MKVEMLLGGTTRFATIEALADATQPITAYQIAMTKGLDPAATYR
                     CLTELSDFRIVESKTGERNQTFYKLSDGPGRAAAEFLRSLKQKTPQVVDLEKWISPEM
                     QSARMGKIVKLDKLDKSIFRNSDGKQDVDKIMSKRISGELSALITSSKITFNELFEQK
                     NNVFILKE"
     gene            993..1601
                     /locus_tag="VGA92_06805"
     CDS             993..1601
                     /locus_tag="VGA92_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEX9846159.1"
                     /translation="MIEDKSGNETRTRFKTSPEYKELREERDSFKRRMFFLGFLTKTL
                     KQNGVEAILVGGQAIDLYTAGTFSTTDIDLLVDDKIIAEKLLNRFGFGKEANGLWLNK
                     DLAIVVQVISQPYSGNSEKLRKFKIKDCELRVAAPEDLIQNRLYSAKFWKSNIQRDME
                     ESISLLRIFADSIDNSYLDKLAKENDIEDYLAKARKYAFEII"
     gene            complement(1661..1738)
                     /locus_tag="VGA92_06810"
     tRNA            complement(1661..1738)
                     /locus_tag="VGA92_06810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1701..1703),aa:Val,seq:tac)
     gene            1795..2817
                     /locus_tag="VGA92_06815"
     CDS             1795..2817
                     /locus_tag="VGA92_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9846160.1"
                     /translation="MSQTDIGFFCSPIGLGHATRDIAIAQFFDKNAVKFVTGDGAAKL
                     FAEYGFSVNDSYKPPKFDVQNGALRGSLKWLWQYYQFYKDCKKIASEFIKNQNPRLVI
                     SDEDFASLTIAQQKNIPSVLITDILQTRFTKGIGSLIEKKMNQSMKDIIQKCNVVIIP
                     EFGQDQENIRHVGPIVRTTDSTREKLRKKFSFNKKTIIVSVGGTDAGEFLIKKTIEVL
                     SRLKGDLELVVVSGPSLKIGDKKNIRNLGFVKELHEMIYASDLVISLAGKSTIDESKA
                     YGTPGIFIPIKNHFEQEDNASREGYSHQDIFRLESLISEKLESKRESLHAEGAEKAYK
                     IIRQFL"
     gene            2875..4290
                     /locus_tag="VGA92_06820"
     CDS             2875..4290
                     /locus_tag="VGA92_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9846161.1"
                     /translation="MEKLVVAKFGGSACGIDGEFVSDIVERIKQLKKDSKVIAVFSAP
                     LTTHEGKRKSLTDLALLIGKNAEEGINPDISAIKKCYEKILGHVSSTYESECKKAIDS
                     FLEKALAAFNDAKQKGSFANEIRSRALAFSGEILMSYVMNYILKSSGIKSDVVSYDIW
                     PIITDYNIESTNFLASESLKRIEPLEHLLKENEIVSIGGFVGKTVDGVETTYERGGSD
                     RTAADLGILFHKKYDTTIDFEKDSAVVSADPRIVTDNLEEIYQLSYNEARLAGMFGMK
                     ILDPIAIKEILENGVDMPIIITNMKNPQKITTIKRRSDNQNGHPLKIVTGKKNCAILK
                     IESEAAQRLLESLEKDKRYSEFVVLSPFTKDGIGFTRILFLDADYVKRNERYILGFDS
                     LATITYNRGVITLIGDQMWRVQQIVSKASSRIGEAGLNILNMDAQEETSRVIIVVDDS
                     ANNIETAIRAVHSERAKITFT"
     gene            complement(4507..4710)
                     /locus_tag="VGA92_06825"
     CDS             complement(4507..4710)
                     /locus_tag="VGA92_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846162.1"
                     /translation="MEPIAFVGAAMVIAGLIGQGFEMKKIRESIRTDEQLSSQNIFAD
                     KRNFKWYAIIAAGFVLWYALSGN"
     gene            4800..>5441
                     /locus_tag="VGA92_06830"
     CDS             4800..>5441
                     /locus_tag="VGA92_06830"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX9846163.1"
                     /translation="MKEVGKIWMNGRLVPFKNAKVHVLTHALHYSTAVFEGIRCYNTP
                     KGSAIFRLPEHVDRLFRSAKLYSMKMQFTKKQITDAIIKTVKTSTLKECYIRPIAYYG
                     YGTMGLTPTPNKVDVAIACWEWKMGESKAGKVSGARCKTSSWLRIDSRSQPMQAKAAS
                     NYANAALARVEALKNGYDESIMLNYQGKVTEGSAENIFVVKNNQIFTPPLTSGI"
BASE COUNT         1882 a         1017 c         1113 g         1429 t
ORIGIN      
        1 agtctcttgt agatttgaga attctgtaag cgtctgccgt gttgaatatc gtcttgcctt
       61 cttcttctag cgtcagtatt atcttcagtt ctctaggtcc cagccttatt tctcttgtaa
      121 ccataatgtc gcctaacaac attatcgtta cacaagtatt aaaatatgtg tcttgtaacc
      181 ataatgtcgc ctaacaacat tatcgttaca cgagtattat gattacccat cgcccatatt
      241 tgcttgcaaa tccaacaaag ctaatgcaca tgtgactgct gttggcgtat cattattgta
      301 gtctccatgt gcaccatcgg cagacagatg tttttccctt atgaatttcc acgaaaatga
      361 tatgaaatcc tcatttggca ctatcagtac caatttttgg atggcttaat tacgataata
      421 tataaataaa actagactta aaatctaagt ctatatatag aagactagac cacactaatt
      481 gaagtacaaa tgaaagtaga aatgctgctt ggaggtacga ctcgatttgc tacaatagaa
      541 gcattagcag atgcaacgca gccaattaca gcctatcaga ttgccatgac taagggactg
      601 gatccagctg caacatatcg ctgtctaaca gaattatcag attttagaat tgttgaatcc
      661 aaaacagggg agcgtaatca aacattctat aaactttcag atggaccggg ccgagcggca
      721 gctgaatttc tacgatcatt aaaacaaaaa acaccccaag tggtagattt agaaaaatgg
      781 atatctcccg aaatgcaatc tgcacgaatg ggaaaaatcg tcaagcttga taaattagat
      841 aaatcaatat tcagaaattc agatggaaaa caagatgttg acaagatcat gtccaaaaga
      901 atatcaggag agttgtctgc acttattaca tcgtcaaaaa taacattcaa tgagttattt
      961 gaacaaaaaa ataacgtgtt tattctaaaa gaatgataga agataaatct ggaaacgaaa
     1021 cgcgtacccg attcaaaaca tctccagaat acaaggaatt gagagaagaa agggattcat
     1081 tcaaacggag aatgttcttt ctaggatttc ttacaaagac attaaaacaa aatggtgtag
     1141 aggccatact tgtaggtggg caggcaatag acctctacac tgcgggaaca ttttctacta
     1201 cagacattga cttactagta gatgataaaa taatagctga aaaattgctt aatagatttg
     1261 gatttggaaa agaagcaaac ggactctggc tcaataagga tttagctatt gttgtgcaag
     1321 tcatatcaca accttacagt gggaattctg aaaaacttag gaaattcaaa attaaagatt
     1381 gtgagttaag agtagctgcg ccagaggatc taatacagaa taggctatat tcagcaaagt
     1441 tctggaaaag caatattcag agagatatgg aagaaagtat ctcattacta agaatatttg
     1501 ctgattctat tgataattca tatctggata aactggctaa ggaaaatgac atagaagact
     1561 atttggctaa ggctagaaaa tatgcatttg agattatcta atttaaaatg acgttaaaca
     1621 aaggctgtag agttctaaaa gggtgcctgc gtctagtgta tggtgggccc acagggattt
     1681 gaaccctgga ttttcgccgt gtaagggcga cgtcataacc gatctggacc atgagcccag
     1741 ccacattcct tggcaaaaac catttaaact ttgagaagag catcacaaaa atcgttgagt
     1801 cagacagata ttggattttt ttgcagtccc ataggacttg gacatgcaac aagagacatt
     1861 gcaattgcac agttttttga caaaaatgca gttaaatttg tcacaggaga tggagctgcc
     1921 aaattatttg cagagtatgg tttttctgta aatgattcat acaagcctcc aaaatttgac
     1981 gttcaaaatg gagcgctgcg aggctcacta aaatggcttt ggcagtacta tcaattctac
     2041 aaagactgca aaaaaatagc atccgagttt atcaaaaatc agaatccaag actggttatt
     2101 agcgatgaag actttgcctc attaaccata gctcagcaaa aaaacattcc atcggttttg
     2161 attaccgata ttttgcagac aagattcaca aaaggaattg gttctctaat tgagaaaaaa
     2221 atgaaccaat ccatgaaaga catcatacaa aaatgcaatg ttgtcataat acctgaattt
     2281 ggccaagacc aagaaaacat caggcatgtt gggccaatag ttagaacaac ggactcgacc
     2341 agagagaagc tacgaaaaaa attctcattt aacaaaaaaa caatcattgt aagtgtaggt
     2401 ggtacagatg ctggagagtt tcttatcaaa aaaacaatcg aagtgctttc aaggctaaaa
     2461 ggagacttgg agcttgttgt tgtatctggt ccttcattga agataggtga taagaaaaac
     2521 atcagaaatc ttgggtttgt aaaagaattg cacgagatga tttacgcatc agatcttgtt
     2581 atctcgcttg caggaaaatc tacaatcgat gaatcaaaag catacggaac accgggaatt
     2641 ttcataccaa tcaagaatca ctttgagcaa gaagataatg caagccggga aggttattca
     2701 catcaggaca ttttcagatt ggaatcacta atttctgaaa aacttgaatc aaaaagagaa
     2761 tcactgcatg cagagggtgc agaaaaagcc tacaaaatca tacgccaatt tctttaatga
     2821 gcctgaatag gacgaatgct taatagtggt ttttagtgtc agaaaaactg cgacttggaa
     2881 aaattagttg ttgcaaagtt tggtggaagt gcatgcggaa ttgacggcga gtttgtatca
     2941 gacattgttg aacgcataaa acaattaaaa aaagattcca aagtaattgc tgtcttttca
     3001 gctcccctca ccactcatga gggaaaaaga aagtcactta cagatctagc actattaatt
     3061 ggaaaaaatg cagaagaggg aataaaccca gatatttctg caataaaaaa atgctatgag
     3121 aagattcttg gccatgtcag tagcacctac gaatcagaat gcaaaaaagc aattgactcg
     3181 ttcttagaaa aagcgcttgc agccttcaac gatgcaaaac agaaaggctc ttttgcaaac
     3241 gagattcgct ctagggcgct tgcgttctct ggcgaaattc tcatgtcata tgtgatgaac
     3301 tatattctca aaagtagtgg gatcaagtcc gatgtggtaa gctatgatat ttggcccata
     3361 attaccgatt acaacataga atcaactaat tttcttgcct cagaatccct caaaagaata
     3421 gaaccgcttg agcatctatt aaaggaaaat gagatagtct caattggagg ctttgttgga
     3481 aaaacagttg acggcgttga aaccacatat gagaggggtg gttctgacag aacagcagca
     3541 gaccttggaa ttttatttca taaaaaatac gacactacaa tagactttga aaaagacagt
     3601 gccgtcgttt ctgcagaccc aagaatagtc acagacaatc tagaggagat ttaccagctt
     3661 tcgtacaacg aggcaaggct tgcaggcatg tttgggatga agattttgga tccaattgcg
     3721 atcaaagaaa ttctcgaaaa cggagtagac atgccaatca taataacaaa catgaagaat
     3781 ccgcaaaaaa tcactaccat taagcgcaga tcagacaacc aaaatggtca cccgctaaaa
     3841 atagttacgg gcaaaaaaaa ctgcgcaata ctcaaaattg aaagtgaggc agcacaaaga
     3901 cttttagaat cactggaaaa agacaagcgc tacagcgagt ttgtcgtttt atcgcctttt
     3961 acaaaagacg gtatcgggtt tacgcgaatt ttgtttttgg atgcagacta tgtgaaaaga
     4021 aatgaaaggt acatccttgg atttgattcg cttgcaacaa ttacctacaa tcgtggtgta
     4081 ataacactga ttggtgatca gatgtggcga gtccagcaaa tagtgtcaaa agcaagctcc
     4141 agaattggcg aggcggggct taacatacta aacatggatg cccaggaaga gacgtcacga
     4201 gtaataatag tagttgatga ttctgcaaat aacatagaaa cagcgattcg tgctgtacat
     4261 tcagagaggg caaaaatcac cttcacctga atgcgacaca aatcgcattg gccccttgtg
     4321 ggtaatttgc ccataagagc cacgctgggt ttgttcttgg gccatcatct agaatcagcc
     4381 cggcgttacc caaaacacgc actactatag tatagtcaac gcgatattta gaggtgatct
     4441 atatagacta tctagagttt tttgcgagct aggcacgaat atgagattta gaaccaaaaa
     4501 tgacatctag tttccactta gtgcatacca caacacaaag ccagcagcaa ttattgcata
     4561 ccacttgaaa ttccgtttgt ctgcaaagat gttttgtgag ctcagctgct catctgttct
     4621 aatagattca cgaatttttt tcatctcaaa gccctgcccg attagtcctg ctatgaccat
     4681 ggctgcaccg acaaaggcta ttggctccat ggattagtta tcacgctcag atatttagtt
     4741 gctgtgcctg cacaccagat aagataattt ttaaaacgcc tgtacatttc caacattcta
     4801 tgaaggaagt tggcaaaatc tggatgaacg gaagactagt gccgtttaag aatgccaagg
     4861 ttcacgttct tacccatgca ttacattatt caacggcagt atttgagggg atacggtgtt
     4921 acaacactcc aaagggttct gcgattttcc gccttccaga acatgttgac aggcttttca
     4981 gatctgccaa gctgtattca atgaaaatgc agttcacaaa aaaacagatt actgacgcaa
     5041 taataaagac tgtaaagaca agcaccctga aggagtgcta catccggccc attgcgtatt
     5101 atgggtatgg tactatgggt ctgaccccaa caccaaacaa agtagatgtc gcaattgcgt
     5161 gttgggaatg gaagatgggc gaatcaaagg caggcaaagt aagcggtgca agatgcaaaa
     5221 cgtccagctg gcttagaatt gattccagat cacagccaat gcaggcaaag gctgcatcaa
     5281 attatgcaaa tgccgcactg gcaagagttg aggcactaaa aaacggatac gacgagtcca
     5341 taatgctaaa ctatcaggga aaggtgacgg agggaagcgc agaaaacatt tttgttgtaa
     5401 agaacaatca gattttcaca ccaccactca catctggaat t
//
LOCUS       DASWKH010000088         7332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1332392, whole genome shotgun sequence.
ACCESSION   DASWKH010000088 DASWKH010000000
VERSION     DASWKH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332392"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGA92_06835"
     CDS             complement(<1..305)
                     /locus_tag="VGA92_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846164.1"
                     /translation="MSDNSPYFFMKEFSSEELVHLLVNKVKELKTEKEKSDELNAKLE
                     EAQQEVARQRDLLQEEVKQKTEDLLKVERLSAIGQLSARIAHDLRNPLTVIQNSSRIL
                     "
     gene            complement(391..579)
                     /gene="spt4"
                     /locus_tag="VGA92_06840"
     CDS             complement(391..579)
                     /gene="spt4"
                     /locus_tag="VGA92_06840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HEX9846165.1"
                     /translation="MVREMACRKCKHVFVGKVCPLCKSSDLTPDWTGVVIVADPQNSA
                     IAKTLGITEKGKYAIRIA"
     gene            complement(579..1187)
                     /locus_tag="VGA92_06845"
     CDS             complement(579..1187)
                     /locus_tag="VGA92_06845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HEX9846166.1"
                     /translation="MFSISTLEDVVRIPPSMFGASLKKAAIAILKEKYESMINAELGY
                     IIMILDSKVDEMGKMIAGDGGTYHRVEFEALTFTPKLQEIVLGEIVDITDFGAFVRIG
                     PTDALLHLSQVMDDYLQSDVKSGMIIAKQSSRTLKVGSTIRARITAVSLGKAATMGKI
                     GITCRQPFLGAEEWIAEEIKKSGKEESDKKEPKEKKPVEVKK"
     gene            1409..2491
                     /locus_tag="VGA92_06850"
     CDS             1409..2491
                     /locus_tag="VGA92_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX9846167.1"
                     /translation="MVGVDQVLAKLATVIDPDLKKDIVSMGMIKDLELNDGNLKFTLE
                     LTTPACPFNDQIEQDVRHAVGELDGIKNFDMNVTAKVMEGRSLDADEAMATVKNIIGV
                     ASGKGGVGKSTVSLNLALALAQSGAKVGLLDADIYGPSIPLMLGMQKAFLEVDNNKLQ
                     PAESNGIKVVSFGFFAEQEHQAAIYRGPIISGILKQFLVDTNWSNLDYLIVDLPPGTG
                     DIPLTLAQTIPITGILTVTTPQEVASNVAVKAIGMFNKLNIPLLGVVENMSYFICPKC
                     SDKHYIFGQGGAKRISEKYNIPFIGEIPLNSGIMEGSDKGRPVMITDPTSPSAEAIRS
                     AAKNVAAQCSILAAKLKEEMQATTQA"
     gene            2495..2986
                     /locus_tag="VGA92_06855"
     CDS             2495..2986
                     /locus_tag="VGA92_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HEX9846168.1"
                     /translation="MKLPENLRKALKSPIGVLVKNQDVSRASLLRFLSANSFIITVGD
                     ATTEKMLYFNLVPSLQIVDGLEKRAKRKPPQSSATKLFCDNPAGEITQQSIDTIRKAF
                     GLKPPVQIIVNGEEDLLVIPACIYAPHNAVVLYGQPNEGLVVVLVTDELRNKTKAILN
                     LMK"
     gene            3112..3303
                     /locus_tag="VGA92_06860"
     CDS             3112..3303
                     /locus_tag="VGA92_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846169.1"
                     /translation="MVLVWIGIAVGILVAIILVKKIIKTSPKSAIIFNLYCKKCGYKT
                     NGLKCPRCESSQQNHQKWK"
     gene            complement(3306..3488)
                     /locus_tag="VGA92_06865"
     CDS             complement(3306..3488)
                     /locus_tag="VGA92_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846170.1"
                     /translation="MPKKRVTFTLDEDLDKKIRSLQVGLITTTNEGWSYSSVLGIVIE
                     EGLKSVSVKKLGKHKH"
     gene            3715..4002
                     /locus_tag="VGA92_06870"
     CDS             3715..4002
                     /locus_tag="VGA92_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846171.1"
                     /translation="MKDQNSNKSVVVLSVEVVLMRKGGPQYHQVLARLERDYECKIFD
                     CFEHPEYLKAVLQDVYGEDYKTIVDNLEVELGEIVSEKGVAGFLEALRQNN"
     gene            4032..4397
                     /locus_tag="VGA92_06875"
     CDS             4032..4397
                     /locus_tag="VGA92_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9846172.1"
                     /translation="MERVFKDIDYVRPFLHEVSNIVGKKWNFRVLWEIRNQKKIRYNE
                     MLYSLHGISPSTLSDVLKYLQKESLVRRVAHGDTPPLKVEYNITQKGLDLIIASSSLI
                     KWTMKERKHISQIPLKNHN"
     gene            complement(4418..4738)
                     /locus_tag="VGA92_06880"
     CDS             complement(4418..4738)
                     /locus_tag="VGA92_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846173.1"
                     /translation="MVRSAAICRDDGTIVCSDHRQSHKPLAPHAEGQASLIRASSRFF
                     NRKINEKSFGKVTYSLTLHEKVARIAVPLHDKYVVLVSADKDTNHTDLIMRDIYPVVE
                     AVKI"
     gene            complement(4893..5828)
                     /locus_tag="VGA92_06885"
     CDS             complement(4893..5828)
                     /locus_tag="VGA92_06885"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9846174.1"
                     /translation="MGMIPPQQDIDERIFPKIENNPKLSFSELKSDLLVSFSESCSEG
                     AVGMVDIVNSTQIAAKIGNSRLAMYYGVFLNSMGAIIQKHGGKVVKNIGDSVLFYFCK
                     PQSSTEFTRPLECALAMISAHDIINKVLSANHLPPLNYRVSLDHGPIVLARTSTSSCE
                     DIFGPPVNMCAKINSLAKPNAVAIGGDMYQIVKHLNYCFEEVKGYSVGFKYKYPVYFL
                     SHNNANNASLITAAIEKTLTEIGTPALEVVIAGLFNNYKCFLSDCYEHPEYLKQTLHD
                     IYGSSHTAIIKSIISKLEPHAYKKPMYDFIESLRI"
     gene            6025..6660
                     /locus_tag="VGA92_06890"
     CDS             6025..6660
                     /locus_tag="VGA92_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9846175.1"
                     /translation="MMRSIYSMNALDGLLAQTLEQVIKKKLGEKTFEKIEDRLQERYK
                     ITAIEAIRDFQRFDATLREFFGTGADAMEKDFLEQIISLDDSKHEKPWLIIENEKICN
                     LVLESYGDKEKKLILDTAFKKPGVILDILERCEIPKSTGYRIINELVEVGLLTEKGFT
                     TTEDGKKVNLYTSLFENVMIDIRQGKVTVRIQLKEEVIKESFFVRILRELT"
     gene            6751..7251
                     /locus_tag="VGA92_06895"
     CDS             6751..7251
                     /locus_tag="VGA92_06895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9846176.1"
                     /translation="MGLVAADPEEIRKLPHKERLGKLWDMFKNNPDEAARWDAVWLAG
                     ELAEETGLKGSIFDEIGQMYAWVLKNDDNSIVRHEVCYQIAGRNMRKQIPELFDAAVN
                     DESDLVRHEATECLSIIHAHEECEKAIAKNLNDPSKAVRETAIFIKKRMARIKNKEYD
                     RMVNSF"
BASE COUNT         2264 a         1430 c         1517 g         2121 t
ORIGIN      
        1 agaattcttg agctgttttg aattactgtc agcggatttc tcaaatcgtg cgcaattctt
       61 gcagacagct gtccaattgc agagaggcgc tcgactttga gcaggtcttc tgttttttgt
      121 tttacctctt cttgcagcaa gtcacgctgc cttgcaacct cttgctgtgc ttcctcaagc
      181 tttgcgttga gctcatctga cttttccttt tccgtcttta gctcctttac cttatttacc
      241 aaaagatgca ccagctcttc actggaaaac tctttcatga aaaagtacgg actgttatct
      301 gacatactat agaactttct ttttaattag taatggattt gtgtgtgttt taaacaaaca
      361 aattgtttaa aagctagttg tgcctgagcc ctatgctatt cttattgcgt acttgccttt
      421 ttctgttatt ccaagcgtct tggcaatggc agaattttgc gggtctgcaa caattaccac
      481 accagtccag tcgggtgtaa gatcagagga tttgcaaaga gggcaaacct ttccaacaaa
      541 aacatgcttg cactttctgc aagccatctc tcggaccatt actttttcac ttctactggc
      601 ttcttttcct ttggctcttt tttatcggat tcttccttac cagatttttt tatctcttct
      661 gcaatccatt cttctgcacc caaaaacggc tgtctacaag taattccaat tttgcccatc
      721 gttgcggcct tgccaagtga cactgctgtg attcttgcac gaatcgtaga gccaaccttt
      781 agtgtgcgac tgctctgctt tgcaattatc atgccggact tgacgtcaga ctgcaaataa
      841 tcatccatta cctgtgataa atgtagtagt gcatcagttg gaccaattct tacaaatgcg
      901 ccaaagtctg taatgtcgac aatttcacct aaaacaattt cctgcaattt cggagtaaat
      961 gtaagtgctt caaactctac tctgtggtac gtgccaccat caccggcaat catctttccc
     1021 atctcatcta ctttggaatc taggatcatg attatgtagc caagctctgc gttgatcatc
     1081 gactcgtatt tttctttgag gatggcaatt gctgcttttt ttagagaggc tccaaacata
     1141 cttggtggaa ttcgcacaac atcctctagg gtagatatag aaaacaacta tgccaaactc
     1201 ttcaagttga atttatgaat ctttggatga atttttgatt attttgataa tctttgcaca
     1261 aaaactgcca aattgctgta tttctgattt gtccgtgctg gagagaattt ctgcttagga
     1321 gtccctaatt tgcaattatt ttggcaaaaa atttcgccgt tttgaaaatt gacctaaatt
     1381 taaataatgt aaatgaagct ttttgtgcat ggttggcgta gaccaagttc tagcaaagct
     1441 tgcaacagta attgatcctg acctcaaaaa agacattgtc tctatgggga tgataaagga
     1501 tctagagcta aacgacggca acctaaaatt cacactagag cttacaacac ctgcatgccc
     1561 atttaatgat caaatagaac aagacgtcag acatgcagta ggcgagcttg acggaatcaa
     1621 aaattttgat atgaatgtga ctgccaaagt aatggaaggt cgctcccttg atgcagacga
     1681 ggcaatggcc acagtcaaaa acataattgg tgtggcaagc ggcaaaggtg gagtcggcaa
     1741 gtccacagtc tcattgaatc ttgccttggc acttgcccag tctggcgcca aagtcggact
     1801 acttgatgct gacatttacg ggccaagcat tcctctgatg cttggaatgc aaaaggcctt
     1861 tttggaagtt gacaacaaca agctccagcc agccgaatct aatggcatca aggtggtgtc
     1921 gtttggattt tttgcagaac aagagcacca ggcagcaatt tatcgcggac caataatttc
     1981 tgggatatta aaacaatttt tggtggacac aaactggtcg aatcttgatt atcttatagt
     2041 tgatctgccg ccaggtactg gcgacatccc tctgacactg gcgcaaacaa ttcctattac
     2101 tggaattctg acagtcacaa caccgcaaga agtagccagc aacgttgcag tgaaggcaat
     2161 tggcatgttc aacaagctaa acatcccatt acttggggta gttgagaaca tgagctattt
     2221 catctgccca aaatgtagtg acaagcacta catctttggc cagggtgggg caaaaagaat
     2281 aagcgaaaaa tacaacattc cattcattgg cgaaattcca ctcaactctg gaatcatgga
     2341 gggctctgac aaaggcaggc ctgtaatgat tacagaccct acatcaccat ctgctgaagc
     2401 aatccgctct gctgcaaaaa acgtggcagc acagtgcagt attttggctg caaagctaaa
     2461 agaagaaatg caggcaacaa ctcaggcata agacttgaag ctcccagaaa atttacgcaa
     2521 ggcgctaaag agcccaatag gagtattagt aaaaaatcaa gacgtatcga gggcgagcct
     2581 tttgcgcttt ctttcggcaa actcctttat cattaccgta ggggacgcaa caactgaaaa
     2641 aatgttgtac tttaatctag ttccatcatt gcaaattgtc gacggcctcg aaaaacgagc
     2701 aaaacgcaag cccccacaga gcagtgcgac aaaactgttc tgcgataacc ctgctggcga
     2761 aattacacag caaagcatcg atacgataag aaaggcattt ggactcaaac ccccagtcca
     2821 aataatagta aatggcgaag aagacctact tgtaatacct gcatgcattt atgcgccgca
     2881 caatgcagta gttctgtacg ggcagccaaa cgaaggacta gtagtggtgc tggtaacaga
     2941 cgaacttaga aataaaacga aagcaatact gaatctgatg aaataggggt atgatgagac
     3001 ggtggctgta tgattcatga ttacacacta aaactctgac cctcttgact tttaaagaga
     3061 ctcgctgaat atttgcgaac ttgatttatt gcagtatgac gtattgatat tatggtactt
     3121 gtctggattg gaattgcagt gggaatccta gttgcaataa ttctggtaaa aaagataatc
     3181 aagacatcgc cgaagtctgc aataatattc aacctatact gcaaaaaatg tggctacaag
     3241 acaaacggcc taaaatgccc aaggtgcgaa agctcgcagc aaaaccatca aaaatggaag
     3301 tgactctagt gcttgtgttt tccaagcttt ttgacagaga ctgacttgag accttcctct
     3361 attactattc caaggactga tgagtaactc caaccctcgt tggtagttgt gataaggccc
     3421 acctgcaggc tccggatttt tttgtccaga tcctcatcta gtgtgaatgt gactcgtttt
     3481 tttggcaaaa ttattcaaat tctgtctttt ctgaaattat ataaaatatc aacttcgcag
     3541 aatcgaagta acattgttcc attaatttta aatgagcaat caggctatgt ggtttgaatt
     3601 gtacgtatta tacgaatcag ttggactaaa tcaggcaaga ctctcatatt ttcagtgttt
     3661 ttgtatcaaa tagacaaaca ctagcttttc ttttgcaaaa aatgttgaac aaagatgaag
     3721 gaccaaaatt ctaacaaatc tgtagtagta ctctccgtag aggtggtgct gatgagaaag
     3781 ggtggtccac aataccacca agtcttggca aggcttgagc gcgactatga atgcaagatc
     3841 tttgactgct ttgagcaccc agagtatcta aaggctgttc tccaggacgt ctatggggaa
     3901 gactacaaaa ccatagtgga caacttggag gttgagcttg gcgagattgt ctcagaaaaa
     3961 ggcgttgccg gttttctaga ggccctcaga cagaacaact aatttttaaa tcataaaatg
     4021 ttttgatatg cgtggagcga gtcttcaaag acattgatta tgtaaggccg tttctgcacg
     4081 aagtgagtaa tattgttgga aagaagtgga actttagggt cctctgggag atacgaaacc
     4141 aaaaaaagat aagatacaac gagatgctat attccttaca tggcatctca ccttcgactc
     4201 tttctgatgt cctaaaatac ctacaaaaag aatccctggt tcggcgcgta gcacatggag
     4261 acacccctcc actcaaagtc gaatacaaca taacgcaaaa aggccttgat ctaatcattg
     4321 caagctcatc tctaatcaag tggacaatga aggagagaaa acacatttct cagattccgc
     4381 taaaaaatca taactgataa aaactgtctg aatctatcta aatctttact gcttctacga
     4441 ctgggtaaat gtcacgcatt atgaggtctg tatggtttgt gtccttgtct gctgaaacca
     4501 agacaacgta tttgtcgtgt aggggaactg ctattcttgc aactttttca tgtagggtga
     4561 gactatatgt taccttgcca aagcttttct cattaatttt tcgattaaag aatcgacttg
     4621 atgcgcgaat tagggatgcc tggccttccg catggggggc cagaggcttg tgactctgtc
     4681 tgtgatcaga gcacactatt gtgccgtcgt cgcggcagat tgcagccgac cttaccataa
     4741 agttagactc aagaatctta ctacaaagca ttcttgctga gtggtaggat agctccctac
     4801 tttctgatag catatgatgt gatgatatga atgccattca tagtgctttg tttgttacaa
     4861 tcaaacagac acattgtttg gtacagaatt tcctagatgc gtagactttc tataaagtca
     4921 tacatcggtt ttttgtaggc atgtggctct agttttgaaa ttattgactt tattattgca
     4981 gtgtgtgatg aaccgtagat atcatgtaac gtctgtttta gatattctgg gtgttcgtag
     5041 cagtcagata aaaagcattt gtagttattg aatagtccag ctatcacaac ttccaaggca
     5101 ggcgtcccga tctcagtcaa cgttttttct attgctgctg ttatgagtga ggcgttgtta
     5161 gcattgttgt gtgaaaggaa atagacagga tacttgtact taaaaccaac cgaatagcct
     5221 tttacctctt caaaacaata attcaaatgc tttacaattt ggtacatatc cccacctatt
     5281 gcaacagcgt tgggcttggc tagggagttt attttagcac acatatttac tggaggtcca
     5341 aatatgtcct cgcacgatga ggtagatgtt cgagcaagca ctattggacc atgatccaaa
     5401 ctgactctgt aatttagtgg aggcaagtgg tttgcagaaa gaaccttgtt aatgatgtca
     5461 tgagccgaaa tcatggccaa ggcacactct aaaggtctgg taaactctgt actactctga
     5521 ggcttgcaaa aatagaacaa aacgctgtcc ccaatgttct ttactacctt tcctccatgt
     5581 ttttgtatta tggctcccat cgagttaaga aacaccccat aatacatggc cagtctagaa
     5641 tttcctatct ttgctgcaat ctgagtcgag tttacaatat ctaccatgcc tacggcgcct
     5701 tctgaacaag actctgaaaa ggacacaagc aaatcagact ttagctctga aaaactcaat
     5761 ttagggttgt tctcaatttt aggaaaaatt ctttcatcaa tgtcttgttg tggaggtatc
     5821 attcccataa ttgagattat gaacattgtt ctagttaaat gtagcgatca actacgctta
     5881 taatatttaa gtatactgca ataattctta aatttgggaa aaattacctg atgattcttt
     5941 tttttaatct aaatattggg aaaaactagc caaaataaag ttaagattga ctagttattc
     6001 tcaatttggg gaaatagata taacatgatg cgctcaatat actccatgaa tgcattggat
     6061 gggcttttag ctcaaacgct agaacaagtc attaagaaga agcttggtga aaaaaccttt
     6121 gagaaaattg aggaccgtct gcaagaacga tacaaaatta ctgccattga ggcaataagg
     6181 gattttcaaa ggttcgatgc taccctgagg gagttcttcg gcactggggc agatgccatg
     6241 gaaaaagact ttttagagca aataatatca cttgatgact ctaaacatga aaaaccatgg
     6301 ctaatcatag aaaatgaaaa gatttgtaat cttgttttgg agtcatatgg ggataaagaa
     6361 aagaaactga tactggacac tgcttttaaa aaaccagggg taattttgga tattttggaa
     6421 agatgtgaaa tcccaaaatc aacgggatat agaataataa atgaattagt tgaggttggg
     6481 ctattaactg aaaagggttt cacaactacc gaagacggca aaaaagtcaa tttgtataca
     6541 tcgttgtttg aaaatgtcat gatcgatatc cggcagggca aagtgacagt aaggattcag
     6601 cttaaggaag aagtcataaa agaaagcttt tttgtcagga ttttgcgtga gcttacctag
     6661 tcaaaaaaat ccacattacc tgaaaatatg gagcagttta agcgtcataa ttatgaaaaa
     6721 tcataaataa ttacaatgtg atggtatgat atgggtctgg tcgcagctga tcccgaggaa
     6781 ataagaaagt taccacataa agaacgactt gggaaattat gggacatgtt caaaaataat
     6841 cctgatgagg cggcacgatg ggatgcagta tggcttgcag gggagctggc agaagaaact
     6901 ggactaaaag ggtctatttt tgatgaaata ggccagatgt atgcttgggt attaaaaaat
     6961 gacgacaaca gcatagtgcg gcacgaagtt tgttatcaga tagctggacg aaacatgaga
     7021 aaacaaattc cggaattatt tgatgctgca gtaaatgacg aaagtgatct agttagacat
     7081 gaggccaccg aatgtctttc aataattcat gcacatgaag agtgtgaaaa agccatagca
     7141 aaaaacctca acgatccaag taaggcggtt cgagaaacgg caatctttat caaaaagagg
     7201 atggcaagaa taaagaataa ggaatatgat aggatggtta actcattctg atttacttat
     7261 attacttttt catgtttgta caatgcatac gtaataatca ttgtatttag taaccaaagc
     7321 atattgacaa ta
//
LOCUS       DASWKH010000089        18034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1724215, whole genome shotgun sequence.
ACCESSION   DASWKH010000089 DASWKH010000000
VERSION     DASWKH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 18034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18034
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724215"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGA92_06900"
     CDS             complement(<1..208)
                     /locus_tag="VGA92_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846177.1"
                     /translation="MKKSVMAAIGSGIIVTIIVSLWLVQIQPATIPTQTSDQNQPIIE
                     QTEVAVSKIKVIASFYPLYEFTKNV"
     gene            299..682
                     /locus_tag="VGA92_06905"
     CDS             299..682
                     /locus_tag="VGA92_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HEX9846178.1"
                     /translation="MPIISISLTDELLSELDKLQKSMGFTGRSEVIRAGIRSFVSEEK
                     QKDELSGNLHAILLAVHNDEFDDIIAGLKHSFEDLIITHLHSKIHGNKCVELFMLDGE
                     AERIGMITRNFKTNKKMDTVKLVTL"
     gene            679..2040
                     /locus_tag="VGA92_06910"
     CDS             679..2040
                     /locus_tag="VGA92_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9846179.1"
                     /translation="MIKSKLGILLGLVIFASAIQIGNSYGHGLGFDTINTASINGKKL
                     TITAQITPTVFSQDVEKRIFVSAIDSLTNQNVENVTYHISLFHEGGLIFSDYFLAEDG
                     IVDMHVTPTADGQIQLRGEKNTETGAWHKTESKPIEIIGPILNSGGLYHFEIEIRTID
                     DPKNIVENPQTYVADVTVVTDHIYEENDKNGNDVKFGIRSYYDKASSFDYNPDTNAIY
                     FEMPFDWDEKNISHVPVVHEEVHFPKEFVDLFVPSYVGKVNGIELFKSSVTVDDYSSE
                     EERIVHFVISQDTLTYLKQAQKAAGVENPQNMQFTLEVSNQLVFPVIAMTKNEEVQVD
                     LSWDPIIIEPNKNTKFIFTFRDAKTGEPLRNTTYELIILQNGKELYKKSGNARIGGDF
                     VDYTFLEKQTGHTSIKFEKLRGTDMRTEFGITVVPEFGSLVFAILPAAIVAALIISKR
                     FRI"
     gene            complement(2037..2639)
                     /locus_tag="VGA92_06915"
     CDS             complement(2037..2639)
                     /locus_tag="VGA92_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846180.1"
                     /translation="MQACAITNEELKHLSEIFNKCISTKTVDALTVILGESVHHKIGK
                     TRLLDLSELDELTPAFADTENMSAVYLKQTGDVNVGVLYYMPEKDGKRFAAKLLGKKR
                     LGAFTKLSKSSLSETGNILSGSFFNALSNEQDCRTSSEVPGFAVDTFRSLLEFPAMDI
                     GIETQSLVACTAEFYSTSDLKLRMLIILDPTSVKKLLEKH"
     gene            complement(2652..3032)
                     /locus_tag="VGA92_06920"
     CDS             complement(2652..3032)
                     /locus_tag="VGA92_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9846181.1"
                     /translation="MSYAPKKILVVDDASFMRTVLKDIIKGNGLATEVIEAGDGVEGV
                     KAYQTQKPDLVTMDVNMPRADGIQALRAIMKIDPAAKVVMVTSVEQKQIVQDAMKLGA
                     RDYIVKPFDRSNVGLVLNKVLRQR"
     gene            complement(3029..3529)
                     /locus_tag="VGA92_06925"
     CDS             complement(3029..3529)
                     /locus_tag="VGA92_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9846182.1"
                     /translation="MSTQVTTFDSFQAVTFALMDSQKKEEYAIPIEQVREIRAVESIT
                     KIPKAKSYVKGIMNLRGLIIPVIDVKEKLGLISDGKSNAAKQRILVAEVSGTLTGLLV
                     DEVDQVMRLQTKDIEAAPQSVLESHNYIKGIAKINNKLIVLLDAVKLLEDSSSAISEV
                     MGENKQ"
     gene            complement(3699..4520)
                     /locus_tag="VGA92_06930"
     CDS             complement(3699..4520)
                     /locus_tag="VGA92_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX9846183.1"
                     /translation="MNSSQTISEQQIKQIERIMLQRVGKDLRQFKRPFLNRRITARIR
                     AVGVIDGSEYAKLLELDSSEPSLLFKSFSINVTEFYRDAFVWKCLSSQIIPQILQNNG
                     TLSLKVWSAGCASGEEPYSLAILLKETMGSKNFKFSIVATDINTEALQRAKKAQYLSA
                     SLKNLSPELIAKYFVKVSDDMYEVSDQIKQLVVFEHGDILSFQIDRANLITCRNVLIY
                     YDKPTQELIFKKFHKTLTKNSYLVIGQDETMMGVEAAKLFSCVLPRERIYTKVLQ"
     gene            complement(4517..5029)
                     /locus_tag="VGA92_06935"
     CDS             complement(4517..5029)
                     /locus_tag="VGA92_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX9846184.1"
                     /translation="MSFTKQTQVNNVIEIAMGGLGITSPEKNILQTFVGSCVAICIYD
                     PQAKIAGMAHVMLPKNNSSDPTPKPEGKFADVALKTLLERLVANGAKLSRLKAKMAGG
                     ANVFQNEGKQTIFNIGLRNVDTIRSTLAEKNISIVSEDVGANNGRWVIFDAKSSEMKV
                     KERTKGVKVI"
     gene            complement(5026..5769)
                     /locus_tag="VGA92_06940"
     CDS             complement(5026..5769)
                     /locus_tag="VGA92_06940"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEX9846185.1"
                     /translation="MSTLSYLSSEETQTLNKIFCQYITSKTVVALSTLLSEPINHNVN
                     ILNNGVTQIKNIKLTPNEIKVCAVRLNGKGDTHIEILYTLNQEHAKKIAAKLLCQTDI
                     REIDEMGASAIQEVANIMTGSFFNALSQGTGFRVDLSTPNFASDELHSLIDNSVKDVM
                     NPIEFAVVADVELVGRTSGTKLHMIIMQNPDNARKLLANHSDKPDTNNQVNSGSVVLA
                     QETAEPYTIGGGNTELDALLGDILQENSS"
     gene            complement(5780..7321)
                     /locus_tag="VGA92_06945"
     CDS             complement(5780..7321)
                     /locus_tag="VGA92_06945"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX9846186.1"
                     /translation="MSQNNYQEMYVSEALEHVETINEALLKLEEEPDKREHLDLIFRS
                     AHTIKGMSATMGYDDTRELCKNIENIFDNMRKGQAKLTPNLANALFKCIDLLREMILD
                     DKKKIDLKPYLQMLEHPEEVQVTAAESSGTKSPTIRVKMSDLDSLVNLVGELVISKMR
                     LEQTLNKGTDDSRQVMTELDRLVTDLQYQSMKLRLVPIDQIFSRFTRLVRDTSSALGK
                     EVRLNMDGSGIELDRTVLDAITDPLLHILRNCVDHGIETPQERATTGKPTSGTIKLTA
                     YGVGDQVAIKIEDDGRGINIDRLKAKAVEKNLLTEEEMQKMSDEEAIDLLGTPGLSTA
                     KEVTDVSGRGVGMDVVITQVEAVGGSVKITTQKDKGTTILLTIPLSVSIIGGLLINVS
                     GDKYVLPLSSISTTVVVEANQVKSIHGQEAIVLREQVVPLIRVAKILGIAENETNTTD
                     KITIVIVDKGGKPFGLVVDSYDRKQEIVIKRLGNESHSSDLFTNATILGDGSVALILD
                     PALLV"
     gene            complement(7308..8390)
                     /gene="cheB"
                     /locus_tag="VGA92_06950"
     CDS             complement(7308..8390)
                     /gene="cheB"
                     /locus_tag="VGA92_06950"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEX9846187.1"
                     /translation="MWRQPELLAKPVSSQTNVLIINDSSYMSTLLKDLISSETIRVYD
                     TAKDGQDALRKIGIQKPDVILLDLEMPRMDGLTFIEEMVKRGTLIPTILVSNFSQEGA
                     KIVLDALENGAIDFVPIAQVSPESISQLKETLISKINTASHCDPYKLVIEKINSLKPK
                     RKPPVSSQAATVVVTIASSTGGPNIVQSILAGLPANIKAGILVVQHMPKGFTSKFAER
                     LNETSEITVREAQDGDLITEGVALVAPGDLHMEIDSQLRVRLVNGPKRFGVRPAANVT
                     MISAAECFGSSTIGVTLTGMGHDGAFGMKTIKRRGGKTIAQDEQSSVVFGMAKAACEL
                     NAVDSLLPPEKIPQAILAEVEKLVTK"
     gene            complement(8581..8832)
                     /locus_tag="VGA92_06955"
     CDS             complement(8581..8832)
                     /locus_tag="VGA92_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846188.1"
                     /translation="MAYGIPNLREIYDRIEAEENTPMSEADGYGWGLDYLKDIMMQLE
                     KLEEKALVKKDPTFYNNIKISIQRAKEAQMELHSKLEQV"
     gene            complement(8934..9623)
                     /locus_tag="VGA92_06960"
     CDS             complement(8934..9623)
                     /locus_tag="VGA92_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9846189.1"
                     /translation="MMAHSAESIQAVSFSIVNPAGKKEDYAVPIEQVREIRAVEKITK
                     VPRSESYIKGIMNLRGLIIPVIDVKEKLGLGSDGKSNSTKQRILVAEINGTLTGLLVD
                     EVDQVMHLQTKDIEAAPQGAFESYNYINGIAKINEKLVILLDVEGLLGGSKEKPESLS
                     NTQSKSQETKSIEKPAQTQKPQQTENTPANAQKPFDDKIPPELAEVFKEDEQGIPPTQ
                     IVREPETIPNN"
     gene            complement(9710..11203)
                     /locus_tag="VGA92_06965"
     CDS             complement(9710..11203)
                     /locus_tag="VGA92_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FlaJ"
                     /protein_id="HEX9846190.1"
                     /translation="MIQIPLLKNFSFSNLQKGDEKFVYFVAFLYSISTGEIGPVDLIK
                     TARETNYGKYSQAFREVFQLGIGWGYGVSRSLEMIAEKISTDKSDPLMQLLIKIAQVI
                     RLGDTLRTFFADQLKSALITYSIIYERKLENEKLFLEMFYTLMSTAAFMIAANSIMSM
                     LMGQENAEGVLVMSFIGVAVSMGAFVFMMYIMFPRDKLGYSTADEDLKFRLKVYMMMG
                     AGTGIGVVLLFTQLLPTTLIVGIAAVPLLYPGLIARKMEQKIKNLNEWYSEFIRHFGE
                     IYATLGSVGGSLEAVLKSDFGPLQKHIERFKNRIKHRIEQKLGFELFSRDTGSQIIAN
                     GNEVISYAMDRGGDMNIAGNYVSDITGKINELRAKRTQTSKTFETIMLVLHALTLSVF
                     GLMNKLTSIFYELVSTVDVQNDAIQLTPIDPELMAVMMPLMIIMTSVISAMALKVAQG
                     GLYKTVFYHIGLLMVIGAVVMFVMDTLLSDYLATHVLDFAPVPAPAP"
     gene            complement(11200..12873)
                     /locus_tag="VGA92_06970"
     CDS             complement(11200..12873)
                     /locus_tag="VGA92_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HEX9846191.1"
                     /translation="MPLDFSQIKDKKFTDEIKKSPHLMNYLEMYTIRGNPLPLFTEQL
                     QSEHKKLKEPNLLYPTADQSYVHINPNTTSDDGYMEYVIVEPDMPDRKIMELADRMFA
                     VHSADLVPPAEITERFNMVENYLKEHTQTTDTSVDYNSLGDIYKLKMLPVNKKDWAGF
                     RYHFLQKRAGTGLLDPFLADPNLEDISIVGAGNVYVIHKSFGALKCPLFLGVEEIDEL
                     IVSMSEQFGKTVSHAKPVIDAVLPDGSRLNVVFGKDISRRGTNATIRKFASTPLSITQ
                     ILTSKALDFREAAYMWMMLAEGSSVFINGETASGKTTTLMALTAFIPSNWKIVTIEDT
                     PEITLPHANWITEQTRDTGNEASSVTMFDLLKASLRQRPNYIFVGEIRGAEANIAFQA
                     MQTGHPVVSTFHAANMTSLIQRITNPPMLIPKTNVENLNIALFQGAVQAPGGKRVRRV
                     LSINEILGYNPDGGNIMFIPVFNWDPGTDEVKFRGKGSSALFVQKVLEKRGMKRRDEG
                     LLYEELQLRANILQKMMEKRIFNFYDVFDSISHCREIGLEAFYKELDSL"
     gene            complement(12899..13600)
                     /locus_tag="VGA92_06975"
     CDS             complement(12899..13600)
                     /locus_tag="VGA92_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9846192.1"
                     /translation="MTEIIPCGNEEVDRQFGGGIPFPTLMLIEGDHGTGKSALAAQFM
                     KGFLESGKRILCVTENTVKEYIENMKAITFNFSTAFLRNRLTIMPLHMYGVQWDKEQS
                     SYLLPVVGKYIGNSFKEHNCVVIDSLSLLTVFSDASRILEFFTQCKYLVARGMSIIIT
                     IHPEDIPPDLRMRVKGGVDVYLKLGSASVGGRDVKTLKVVKLIGAKENTESGFAFDVD
                     MTFGIKIVPISMANA"
     gene            complement(13718..14896)
                     /locus_tag="VGA92_06980"
     CDS             complement(13718..14896)
                     /locus_tag="VGA92_06980"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HEX9846193.1"
                     /translation="MQRTYKFRIYPNHNLSAKLHCAAGACRWVYNKMVEKIHSDGLHT
                     RNDLCYFLMELKEAEPWLYEHHSKMLQMVAVQVDGAQKSLIQLRKNGNKTGSLKFARL
                     SEYSTFTYNQSGFELKDGFLHLSKIGKIKLNQHRQIPENSDIKQITISKSRSGKWYAC
                     VVVDIDVALPKISLKRSVGIDVGIKNFAYDSDGFATPNPLNLQKMLKPLVRAQRKISR
                     RQPDSNNHKKAVRFYQIIHERIKNRRKDFLHKISTIYAKKYDVIFVERLAKLNMVKNH
                     RLARNILDSGWGIFTNMLDYKTMRVEVPARNTTMQCSRCGNMVPKSLAVRTHKCDACG
                     LILDRDHNAAINILIKGLEIFGLSPQAIISVPQELRELTPVEISKRSRKQEATIFSEW
                     "
     gene            complement(15020..15532)
                     /locus_tag="VGA92_06985"
     CDS             complement(15020..15532)
                     /locus_tag="VGA92_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846194.1"
                     /translation="MGLSIVVSGGIVLFALVYILLTIPGVVNQTVSITRASTDISEVE
                     NSILRTNIEIDSLDASSGSSVINFTVNSLGVEKLWNFAKFNLIITYPSASGTNTESLS
                     YAGICSGNPSSGTWCVASISDDNVDPNILNTDETLNARSRVSQSLISGTVNTAFSTDN
                     GIVAGRSDSI"
     gene            complement(15552..16034)
                     /locus_tag="VGA92_06990"
     CDS             complement(15552..16034)
                     /locus_tag="VGA92_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX9846195.1"
                     /translation="MGAGIMTEGILIIASIVVATTMAGIIMSQVGTFESTFTATSESQ
                     KNEMLTKLKVIHAMYNSTASPETLEVWVKNIGIDPITAPTSMDVYYGETGSAQRVAYD
                     ESGGQDTWRFEPSTPPTMIQKMDTIQIKITDSDLAAGNTYVVRITAPNGVYSDYVLST
                     "
     gene            complement(16037..17266)
                     /locus_tag="VGA92_06995"
     CDS             complement(16037..17266)
                     /locus_tag="VGA92_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846196.1"
                     /translation="MFVAGSVIGMYGLVLYEVFPEYLFDINYSLYYFVIPVSAIASVL
                     VALPKFLGGNSAPKPQAAVSEEDPLLTQIIGDESQPAQSATSEAQSENIDISKVGGSN
                     AELDALLAGGSDATSDTVNSDTTVAADTPAFDESRIRELIDQKFEPVEKDLTTFKKDL
                     NKIKEDMKITKESVDTLTESFEGTLTDMKAFQAEISNPLNFMRKYFEALDLSNLSDPS
                     LPLRQGILQTQQMAQNYANNQSQNVTPNTQNQNNPMPIIMQAAGSNTTTSVQNPPSPS
                     AIVKGSEMDNLVKGSDTNNPMESVMKPLFNGSLSVANMMAIIELVGEMFEEKGDDCID
                     LLVEQCKLMGLKPEDEHTIYNIIDMLKNSGMSVEESISQLYRFAKIVGLNDKEADAYY
                     ARLSAQKRPRRQNTGSD"
     gene            complement(17353..>18034)
                     /locus_tag="VGA92_07000"
     CDS             complement(17353..>18034)
                     /locus_tag="VGA92_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9846197.1"
                     /translation="VIGVESAIVMIAFVIVAAALAFVVLNMGFSTTQKAKTTIVSGLG
                     EASSSLEVSGKVFGSGHVSAAKLNVTAFPIKIASGGESVNLANTTAAVKYLSNTLSYD
                     NIYKGTLTTTAQDDGDGTADGQWLQLSEAAETAKQEAFINDNPFESDGSSWPSETKAF
                     IYWTVNSNNNDILDQGEHAVLAIVYAADDRPSALDKVRAEVLVPSGAALTVERQVPSI
                     TTEVVDLG"
BASE COUNT         4612 a         3668 c         3713 g         6041 t
ORIGIN      
        1 caacattttt tgtaaattca taaagtggat aaaatgatgc aatgacttta atttttgaaa
       61 cggcaacctc ggtttgttct attatgggtt gattttgatc agatgtttgg gttggaattg
      121 ttgcaggttg gatttgaact agccacaaac taactattat tgtgactata atgccgctac
      181 caattgcagc cattaccgat tttttcatat ttttttgtgc gtaaagttat taataaacct
      241 ataaaaagtt aataacttta atggctatat ttataacaaa tttaataata aattcagcat
      301 gccaatcatc tcaatctcgc taactgacga gttgctatca gagcttgaca agctgcaaaa
      361 gtccatgggc tttactggca gatccgaagt aattagggct gggatacgta gctttgtttc
      421 tgaggaaaaa caaaaagacg agctttcagg aaacctacac gcaattttac ttgctgtcca
      481 taatgacgag tttgacgata ttattgcagg actcaagcat agctttgagg atctgataat
      541 tactcatcta cacagcaaaa ttcacggcaa caagtgtgtt gaattattca tgctagatgg
      601 tgaggctgaa agaattggca tgattacacg aaatttcaag accaacaaaa aaatggatac
      661 tgtcaagctg gttacattat gatcaaaagt aaacttggca ttttgcttgg tttagttatt
      721 tttgcatcag caatccaaat tgggaacagt tatggtcatg gacttgggtt tgataccata
      781 aatacagcaa gtattaatgg aaaaaaactc acaattactg ctcaaataac acctactgtt
      841 ttttctcaag atgtggaaaa aaggatattt gtcagtgcaa ttgattctct aacgaatcaa
      901 aatgttgaga atgtaacata tcacattagc ctatttcatg aaggaggttt gattttcagt
      961 gattactttt tggcagaaga tggtatagta gacatgcatg taactcctac tgcagatggt
     1021 cagatacaac tcagaggaga aaaaaatacc gagacaggcg cttggcataa aacagaatca
     1081 aaaccaattg aaattattgg accaattctc aattctggcg gcctttatca ttttgaaatt
     1141 gagattagga ccattgatga tccaaaaaac attgtagaaa atccgcaaac ctatgttgca
     1201 gatgtgaccg tagttacaga tcatatctat gaagaaaatg acaaaaatgg aaacgatgtc
     1261 aaatttggaa tcagatctta ttacgataag gcatcaagct ttgactacaa tccagacacc
     1321 aatgccatat actttgagat gccctttgat tgggatgaga aaaacatctc gcacgttcct
     1381 gtagttcatg aagaggttca ttttccaaag gagtttgtgg acctttttgt gcctagctat
     1441 gtaggaaaag taaacggaat tgagctattc aagtcatctg ttactgtaga tgactactct
     1501 agtgaggaag aaaggattgt gcactttgtt atttctcagg acaccctcac ttacttaaag
     1561 caggcacaaa aggcagcagg cgttgaaaat ccacaaaaca tgcagtttac attagaggta
     1621 agcaatcaac ttgtctttcc agttattgcc atgaccaaaa acgaggaagt ccaagttgat
     1681 ctttcctggg atccaataat aattgaacca aacaagaaca caaaattcat ctttactttt
     1741 agagacgcaa agacgggtga accgctccgc aacacaactt atgagctgat catcttgcaa
     1801 aacggaaaag agttatacaa aaaatcagga aacgcacgaa ttggtggaga ttttgttgac
     1861 tataccttct tggaaaaaca aactggtcat acatcaatca agtttgaaaa gctgcgtggc
     1921 acagacatga gaacagagtt tggaataacg gtggtgccag aattcggttc acttgtgttt
     1981 gccatattgc ctgcagcaat tgtcgctgcc ttgattatca gtaagagatt tagaatctag
     2041 tgtttctcaa gcaatttttt gacgcttgtt ggatctaaaa tgattaacat tcgaagcttt
     2101 agatcacttg tcgaataaaa ttcggcagta catgcaacca gtgattgtgt ttcaattcca
     2161 atatccattg ctggaaactc taagagtgat ctgaatgtat caactgcaaa gccagggacc
     2221 tcagacgaag ttctgcagtc ctgctcattt gataatgcgt taaaaaacga gccggaaaga
     2281 atgtttcccg tttccgaaag agatgatttg cttaactttg taaatgcacc tagccttttt
     2341 ttgcccaaaa gctttgctgc aaatcttttg ccgtcttttt caggcatata gtatagtacg
     2401 cctacgttga catcccctgt ctgcttcaga tatacggccg acatgttttc agtgtctgca
     2461 aatgctgggg tcagctcatc aagctccgat aagtcaagca gtcttgtttt gcctattttg
     2521 tggtggactg actcgcccaa aatcacggtt aacgcatcta ctgtcttagt tgagatgcat
     2581 ttgttgaaga tttcagaaag atgcttgagt tcctcgtttg ttatggcgca tgcttgcatt
     2641 agtttttgcc cctatcgttg tcttagtacc ttgtttagaa caaggcccac attgcttcga
     2701 tcaaatggtt ttacaatgta atctcttgca ccaagcttca tggcgtcttg gactatttgc
     2761 ttttgttcta cagatgtcac cattactacc ttggctgccg gatcgatctt cattattgca
     2821 cgtagtgctt gaattccatc tgcacgcggc atgttaacat ccatggtaac aagatcaggc
     2881 ttttgggtct ggtatgcctt taccccctct acaccgtctc ctgcctcaat tacttctgtt
     2941 gcaagaccat ttccttttat gatgtctttt agaacagtcc tcataaacga ggcatcgtca
     3001 acaacaagga ttttctttgg ggcgtagctc attgtttatt ttctcccatt acttcgctga
     3061 tcgcagaaga tgaatcctca agtagtttta cggcatcgag tagaacaatt agtttgttgt
     3121 tgattttggc aattccttta atgtagttgt gtgattctag tacactttga ggtgctgcct
     3181 ctatgtcctt tgtctgaagg cgcattacct ggtcaacttc gtcgactaga aggccggtga
     3241 gtgtaccact tacctcggca actaggattc tctgctttgc agcgtttgat tttccatctg
     3301 agatcagtcc gagtttttcc tttacatcaa ttactgggat gatgaggccg cgaaggttca
     3361 ttattccttt tacgtaagac tttgcctttg gaattttggt aattgactct actgcgcgga
     3421 tctcgcgtac ctgctctatt ggtattgcat attcttcctt tttctgactg tccatcaaag
     3481 caaaagttac tgcctgaaat gagtcaaaag ttgttacttg tgttgacatt aaatgatccc
     3541 ctgttttgct tgtgtaataa ctagagctga gatatatgat gaattcatga aggttgtttt
     3601 tctaataaaa taataatcta gagtgtgttt gcgatgatca gacaatatca gtgaaacata
     3661 ttttagaaat gagggggttg ttttacaaag attagtcatc attgtagcac cttagtgtag
     3721 attctttcac gtggaagtac acatgagaat aactttgcag cctctacgcc catcatagtt
     3781 tcatcttggc ctatgacaag atagctgttt tttgttaggg ttttgtgaaa ttttttaaag
     3841 attagctctt gagtaggctt gtcataatag atcaaaacgt tacggcatgt aattagattg
     3901 gcccgatcta tctgaaaaga caaaatgtca ccgtgttcaa agactacaag ttgctttatt
     3961 tgatcactta cttcgtacat atcatcactt accttgacaa aatattttgc aatcagctca
     4021 ggagacaagt ttttcaaact tgctgaaagg tattgggctt tttttgctcg ctgcagtgct
     4081 tctgtgttga tatcggttgc aactatactg aatttgaaat tttttgatcc catggtttct
     4141 ttgagcaaaa tagcaaggct gtagggttct tctcctgagg cgcaaccagc actccaaact
     4201 ttcagtgata aggtaccgtt gttttgtaga atttgtggaa ttatttgtga agaaagacac
     4261 ttccagacaa atgcatctct gtaaaattcg gttacattaa ttgaaaagct cttaaacaat
     4321 aaagatggct cacttgaatc taactctagc agctttgcat attcagagcc gtctataaca
     4381 cctactgccc gtatgcgtgc agtaatgcgt ctgtttagaa atggtctttt gaactgtcgg
     4441 aggtcctttc caactctttg tagcattatt ctttcaattt gttttatttg ttgttcagag
     4501 attgtttggc tcgaattcaa atgactttca caccctttgt tctttccttt accttcattt
     4561 cagatgactt ggcatcaaaa attacccatc tgccattatt ggctccaaca tcttcagaca
     4621 ctattgagat atttttttct gcaagtgttg atcggattgt gtcgacattt cgcagtccta
     4681 tgttaaagat cgtctgtttt ccttcgtttt ggaaaacatt ggcgccacct gccatctttg
     4741 cctttagtct gctcagcttt gctccatttg caacaagtcg ctctaacaaa gttttgagtg
     4801 caacatcagc aaacttgcct tccggttttg gggtaggatc agaagaatta ttttttggaa
     4861 gcatcacatg agccatgccg gcaatctttg cttgtggatc atagatgcag attgcaacac
     4921 aagagccaac aaacgtttgc agaatgtttt tttccggaga agtgattccc aagcccccca
     4981 ttgcaatttc aataacgtta ttgacttgag tttgttttgt aaaactcaac tactgttctc
     5041 ctgtagtatg tcgcccagta atgcatcaag ttctgtgttc ccaccgccaa ttgtatacgg
     5101 ttctgcagtt tcttgggcca aaaccaccga tccagagttt acttgattat ttgtatcagg
     5161 tttatcagaa tggtttgcaa gtagctttct tgcgttgtct gggttttgca ttataatcat
     5221 gtgtaatttt gtcccactgg ttcttccaac tagttcgaca tcagctacta cagcaaattc
     5281 gattggattc attacgtctt taactgaatt atcgattagc gagtgtaact catcactggc
     5341 aaaattcggc gtggaaagat caacacgaaa cccagttccc tgagagagtg cgttaaaaaa
     5401 cgagcctgtc attatgttgg ctacctcttg aattgcagag gcgcccattt cgtcaatttc
     5461 acgtatgtcg gtttggcaga gtagctttgc agcaattttt tttgcgtgtt cttgattgag
     5521 cgtgtataga atctctatgt gggtatcgcc tttgccgttt agtctaactg cacacacttt
     5581 gatttcgttt ggggtcagct tgatattttt gatctgagta actccgttgt ttaagatgtt
     5641 gacgttatga tttattggct cagagagcag tgtagatagt gcaactactg tttttgatgt
     5701 aatgtattga caaaaaattt tgttaagtgt ttgcgtttct tctgagctta gataggacaa
     5761 tgtactcata gtataacacc taaactaaaa gggctggatc aagaattaat gcaacactac
     5821 catctccaag aatggttgca ttggtgaaaa gatctgacga gtgtgattcg tttccaagtc
     5881 gtttaattac aatttcctgt ttacgatcgt aagagtccac tacgagccca aatggttttc
     5941 ctcccttgtc gacaattaca atagtgattt tatcagtggt gtttgtctcg ttttctgcaa
     6001 taccaagaat ctttgccacc ctgattagtg gtacaacctg ttcacgtaag acaattgctt
     6061 cctggccatg gatgcttttg acttgattgg cttcgactac cacagtagta gaaatactag
     6121 atagtggtag gacatacttg tcgcctgaga cgttaattag taatccacct attattgaaa
     6181 cgcttaatgg aattgtcaaa agtatagttg ttcctttgtc cttttgggtt gtgattttca
     6241 cactaccgcc tactgcttcg acttgggtta tgacgacatc catgccaact cctctaccag
     6301 agacatctgt tacctccttt gcagttgaga ggccaggtgt gcctaggagg tcgattgctt
     6361 cctcatcaga cattttctgc atttcttctt ctgtgaggag attcttttca acggcttttg
     6421 ctttgagccg gtctatgttt attccccttc cgtcatcttc aattttaatt gcgacttgat
     6481 cacctacccc atatgcagtt agttttatgg taccagatgt tggcttgcca gtagtagcac
     6541 gctcctgtgg cgtttcaatt ccatgatcca cgcaatttct tagaatatgt aagagcggat
     6601 cagttattgc gtcaagaact gttctgtcaa gctcaattcc agacccatcc atgttaaggc
     6661 gtacttcttt tcctagtgca gacgatgtgt ctcggacgag tctggtaaat ctgctaaata
     6721 tctggtcaat tggaacaagt ctaagcttca ttgactgata ctgcaagtca gtaacaagtc
     6781 tatcaagctc cgtcattacc tggcgagaat catcagttcc cttgtttagt gtttgttcta
     6841 gtctcatctt tgagatgaca agctcgccaa ccaagtttac tagcgagtcg aggtctgaca
     6901 tttttacacg gatggttggc gattttgtgc cacttgattc agctgcggtt acttgaactt
     6961 cttcaggatg ctcaagcatc tgaagatatg gtttgagatc tattttcttt ttgtcatcta
     7021 gaatcatttc tcgtagcaga tcaatgcact tgaaaagcgc attggccaag tttggggtaa
     7081 gctttgcctg accctttcgc atgttatcaa aaatgttttc aatgtttttg cacagctctc
     7141 tggtatcgtc atatcccatt gtagcagaca tgcctttaat ggtgtgagcc gaacggaata
     7201 tcagatcaag atgttctcgt ttgtcaggct cttcttctag ttttagcaac gcttcgttga
     7261 ttgtctctac atgctctagt gcttctgata catacatttc ctgatagtta ttttgtgaca
     7321 agtttttcaa cctctgctaa aattgcttgt ggaatttttt ctggcgggag taaggaatct
     7381 actgcgttta gttcacaggc tgcctttgcc atgccaaaga ctacagacga ttgttcgtct
     7441 tgggctattg tttttccgcc ccttcttttt attgtcttca tgccaaatgc gccatcatgg
     7501 cccataccag ttaaagtaac accgattgtg cttgagccaa aacactctgc tgctgagatc
     7561 atagtcacgt ttgctgcagg tctaacacca aaacgctttg ggccattgac aagccttact
     7621 cgaagttgtg aatctatctc catgtgaaga tctcctgggg ctacaagtgc aactccttct
     7681 gttatgagat caccgtcttg ggcctcccta actgtgattt cggatgtctc gttgagtctt
     7741 tcggcaaatt ttgatgtaaa gccttttggc atgtgttgaa caactaaaat tcctgccttg
     7801 atgttggcag gaagccctgc aagtattgat tggactatgt ttggtcctcc tgtagatgat
     7861 gcaattgtca caacaactgt tgcagcttgt gatgagacgg gtggttttct ttttggcttt
     7921 aggctgttga ttttttcgat tacaagcttg tatggatcac agtgtgacgc agtattaatt
     7981 tttgagatga gtgtctcttt aagctgggat atgctttctg ggctcacttg agcaattggg
     8041 acaaaatcaa ttgcgccgtt ttcaagtgca tcgagaacta tttttgcacc ctcttgacta
     8101 aagttgctaa ccaagattgt tggaattaat gttcctcgtt ttaccatctc ctcaataaag
     8161 gtcagcccat ccattcttgg catttctaaa tcaagtaaaa ttacgtcggg tttttgaata
     8221 ccgatttttc ttaatgcatc ttgcccgtct tttgcagtat cataaacacg gattgtttca
     8281 cttgagatca aatccttgag taatgtactc atgtagctgg agtcattaat tattaagaca
     8341 ttagtctgtg atgatacagg ctttgctaac aactcaggtt gcctccacat tgtaattcag
     8401 ggtatgaata atcaggtgta ccacccacgt gaagtcctaa taaaattaga gattggcaga
     8461 tgaaatggca ttaaatttcg taaaatcaaa tatcgatata tcatcaagta tgtttgatat
     8521 gatcatacaa aattttgaca ctaaagagac atcccaaagt aaaaagaatt tgttattttc
     8581 ttagacttgc tcaagtttgc tgtggagctc catttgtgcc tcttttgccc tctgaatcga
     8641 tatcttgatg ttattgtaga atgtaggatc cttttttacc aatgcctttt cctcaagttt
     8701 ttcaagctgc atcattatgt ctttgaggta gtctagtccc cacccatagc catcagcctc
     8761 actcatcgga gtgttttctt ctgcttctat tctgtcgtag atctccctaa gatttggtat
     8821 cccatacgcc atgtgagaag ccgtgtccca aattgtatta aactgaaagg aattttttgg
     8881 aaaagatcca ctccagaatg gcacaaattt agatgatttt gcccattttg gaactagtta
     8941 ttgggaatgg tttcaggttc tctgactatt tgagttggag ggattccttg ttcgtcttct
     9001 ttgaagactt cggcaagttc cggcgggatt ttatcatcaa agggtttttg tgcgtttgca
     9061 ggagtgtttt ctgtttgttg aggtttttgg gtttgggctg gtttttcgat tgattttgtt
     9121 tcttgagatt tagattgtgt atttgatagg ctttctggct tttcctttga gccaccaagc
     9181 aggccttcaa cgtccaatag aattaccaat ttttcgttaa ttttggcaat gccgttgata
     9241 taattatatg attcaaatgc gccttggggt gctgcttcta tgtcctttgt ctgaaggtgc
     9301 attacctggt caacttcatc gactagaagg cctgttagtg taccgttaat ttcggcaact
     9361 aggattctct gcttagtaga gtttgatttt ccatcagagc ccagtccgag tttttccttt
     9421 acatcgatta ctgggatgat gaggccgcga aggttcatta ttccttttat gtaagactct
     9481 gatctaggca ccttggtaat tttttcaacc gcacggattt ctcgcacctg ttctattgga
     9541 actgcatagt cttctttttt gccagctgga ttgactatac taaatgacac tgcttggatg
     9601 gattctgcac tgtgcgccat cattgtatgt ttacctcaat tcggcacatg aattcattat
     9661 tttgtaattt ggatatagga tgatgtgtgt ttagtttatt catacaaaac tatggggctg
     9721 gtgcagggac tggtgcaaag tctagcacat gggttgccaa atagtccgat agtagtgtgt
     9781 ccatcacaaa cattacaaca gcacctatca ccattagaag gccaatgtga tagaagacgg
     9841 tcttgtatag accgccttga gctactttta atgccattgc agatattaca gaggtcatta
     9901 taatcatcag tggcatcatt actgccataa gttctgggtc aattggagtg agttgtattg
     9961 catcgttttg aacgtccaca gtacttacaa gctcgtaaaa tattgaggtg agtttgttca
    10021 ttaagccaaa gactgaaagt gtgagtgcat gaaggaccag catgatcgtc tcaaatgttt
    10081 ttgacgtctg agttcgcttt gccctaagct cgtttatctt gcctgtgata tcggatacat
    10141 agtttcctgc aatgttcata tcaccacctc tgtccattgc gtatgaaatc acttcgtttc
    10201 catttgcaat tatttggcta ccagtatccc tagaaaacag ctcaaagccg agtttttgct
    10261 caattctatg cttaattctg tttttaaaac gctcaatatg cttctgaagt ggtccaaaat
    10321 cgctttttag taccgcttct aaagatcctc caacggatcc gagtgttgcg tagatctctc
    10381 caaaatgtcg gataaattct gaataccact cgtttaggtt ttttattttt tgttccatct
    10441 ttcttgcaat caatccaggg tatagtagtg gcactgccgc aatacccaca atcagcgtag
    10501 ttggcaaaag ctgtgtaaac aaaagaacca caccgatccc tgtgcccgca cccatcatca
    10561 tgtaaacttt gagtctgaat tttaaatcct catccgctgt tgaataacca agcttgtcac
    10621 gtgggaacat tatgtacatc ataaaaacaa atgcgcccat gcttactgca acaccgataa
    10681 atgacattac aagaactcct tctgcgtttt cttggcccat caacatgctc ataatcgagt
    10741 ttgctgcaat catgaatgcc gcagtagaca tgagtgtgta gaacatttct aagaatagtt
    10801 tttcgttttc aagttttctt tcgtatatga tactataggt tattagtgca gattttagct
    10861 gatcggcaaa aaatgtcctc aatgtgtctc cgagtcttat gacttgggct attttgatca
    10921 aaagctgcat gagtggatca cttttgtctg tggagatctt ttctgcaatc atctccaatg
    10981 atctggatac gccataaccc catccaatcc caagctgaaa aacttccctg aatgcttgac
    11041 tgtattttcc ataattggtt tcacgggcgg ttttgattag atcgactggg ccaatttctc
    11101 ctgtggaaat actgtacaaa aaagccacaa aataaacaaa tttttcatca cctttttgta
    11161 gatttgaaaa tgaaaagttt ttgagaagtg ggatctgtat cacagcgaat ccagctcctt
    11221 gtaaaatgcc tcaaggccaa tttctctgca gtgtgatata gagtcaaaca catcataaaa
    11281 gttaaagatt cttttttcca tcattttttg caagatgttt gcgcgaagct gcagctcttc
    11341 atacagcaag ccttcgtccc ttcttttcat gcctcgcttt tcgaggactt tttggacaaa
    11401 gagcgcactg cttcctttgc ccctgaactt tacctcgtct gttcctggat cccagttgaa
    11461 gacaggaatg aacatgatgt ttccaccatc tgggttatag cctaggattt cgttaattga
    11521 aagaacacgt cttactcttt ttccgccagg ggcctgcacg gctccctgaa acaaggcgat
    11581 gtttaggttt tcgacgtttg tttttgggat tagcattggg gggtttgtaa ttctttgaat
    11641 gagtgaggtc atgtttgctg cgtgaaacgt acttaccact gggtgacctg tctgcatagc
    11701 ctgaaaggca atgtttgctt ctgcacctct gatttctcct acaaagatat agttcggacg
    11761 ctgtcttaat gatgccttta gtagatcgaa cattgtgaca cttgatgcct cgtttcccgt
    11821 gtcccttgtc tgctctgtaa tccagtttgc gtgaggcagg gtaatttcag gcgtatcttc
    11881 aattgtcact attttccagt ttgatgggat gaatgcggta agtgccatga gcgtagtagt
    11941 tttaccagat gctgtttctc cgttgataaa tacgctagat ccttctgcga gcatcatcca
    12001 catgtaagca gcctcgcgaa agtcgagtgc ctttgatgtt aagatctgtg taatagaaag
    12061 tggcgtgctt gcgaattttc tgatagtcgc attggttccc cttctgctaa tgtccttacc
    12121 aaatacaacg ttaagtctgg atccatcagg aagtactgcg tcgattactg gttttgcgtg
    12181 agatacagtt ttgccaaatt gttctgacat gctaactatt agttcgtcaa tttcttccac
    12241 gcccaaaaac aaaggacatt tgagtgcgcc aaatgacttg tgtatgacat agacgtttcc
    12301 tgctccaaca atggaaatat cttcaaggtt tggatctgca aggaatggat caagaagccc
    12361 agttcctgct cttttctgca agaaatggta tcggaatcca gcccagtcct ttttgtttac
    12421 tggcaacatc tttagcttgt aaatatcacc taggctgttg taatcaacag atgtatcagt
    12481 tgtctgggta tgttctttga gatagttttc aaccatgttg aatcgttccg tgatttcagc
    12541 tggtggaact aaatctgctg aatggactgc aaacatcctg tctgcaagct ccatgatttt
    12601 tctatcaggc atgtctggct caactatgac atattccatg tagccatcat cggatgtggt
    12661 gttcggattg atgtggacgt atgactgatc tgcagttggg taaagcaggt ttggctcctt
    12721 gagttttttg tgctctgatt gtagctgttc agtaaacaat ggaagtgggt ttcctcttat
    12781 tgtgtacatt tcaagatagt tcataagatg cggtgacttt ttgatctcat cggtgaattt
    12841 tttgtccttt atttgcgaaa aatcgagggg caagttctat ttgacctcca tgacccacct
    12901 aggcgtttgc catcgagatt gggacaatct ttatgccaaa agtcatgtct acatcaaagg
    12961 caaaaccaga ttctgtgttt tctttggcgc caattagttt tactaccttt agtgtcttga
    13021 catcccttcc accaacagaa gctgagccca gttttaggta tacatccact ccgcccttta
    13081 ctctcattct aagatctggt ggaatgtctt ctggatgaat cgtaataatg atactcattc
    13141 cacgtgcgac aagatatttg cattgtgtaa agaattccaa gattctactt gcatcagaaa
    13201 aaacagtcag aagagacagt gaatcaatta ctacacagtt gtgttctttg aagctgtttc
    13261 caatgtattt gccgacaact gggagaagat atgaagactg ttctttgtcc cactgtactc
    13321 catacatgtg gagcggcatg attgtcaatc tgtttctcaa aaatgctgtt gaaaagttaa
    13381 acgtgattgc cttcatgttt tcaatgtatt cttttaccgt gttttcagta acgcaaagaa
    13441 ttcttttgcc agactccaaa aatcctttca taaattgggc agccagagca cttttgcctg
    13501 ttccatggtc cccctcaatt agcattagtg ttggaaatgg tattccgccg ccaaactgtc
    13561 tgtctacttc ctcatttcca cacggaatta tttcagtcaa ctaaattcct ccaattagtg
    13621 tctataacat acggtaggtc cgctacaatg ttcaatattt attctgaaaa aaaatttgct
    13681 ataagatcgt gtttggtttt ttcgaacgaa tagtgagcta ccactcgcta aagatcgtgg
    13741 cttcctgctt ccttgacctc tttgagatct ctacaggcgt caattcccgc agttcctgcg
    13801 gtactgatat gatcgcttga ggcgacagtc caaaaatttc taatcctttt atcaaaatat
    13861 tgattgctgc gttgtgatcc ctgtcaagta tcagaccaca cgcatcacat ttgtgagttc
    13921 gaacggctaa agattttggc accatgttac cgcaccttga acattgcata gttgtgttcc
    13981 ttgcagggac ctcaactctc atggtcttgt agtcaagcat gtttgtgaat atgccccatc
    14041 ctgaatccag aatgtttctt gccagtctgt gattctttac catgttcagt tttgccagtc
    14101 tttctacaaa tatcacatcg tattttttag cataaatagt ggatattttg tgcaaaaagt
    14161 cttttctgcg gtttttgatt cgctcatgaa ttatctgata gaatcgtact gcctttttgt
    14221 ggttgtttga atctggttgt cttcttgata tttttctttg agctctgact aatggcttta
    14281 gcatcttttg caagtttaat gggtttggtg ttgcaaaccc atccgaatcg tatgcaaagt
    14341 ttttgattcc tacatcgatt ccgactgatc gtttgagcga tatctttggc agagcgacgt
    14401 ctatatccac aacgacacat gcataccatt ttcctgatct tgattttgat atggtgattt
    14461 gtttgatgtc cgagttttcg gggatttgcc tgtgctggtt tagtttgatt ttgcctattt
    14521 tggagaggtg taaaaagccg tccttgagtt caaagccaga ctgattatat gtgaatgtac
    14581 tgtattctga gagtcttgcg aatttgagtg agcctgtttt gttgccgttt tttctcagct
    14641 ggattaatga tttctgtgca ccgtctactt ggactgccac catctggagc atcttggagt
    14701 ggtgctcgta cagccacggc tcagcctcct ttagctccat cagaaaatag cacagatcgt
    14761 ttcttgtatg aagcccatca gagtgtattt tttctaccat tttgttataa acccacctgc
    14821 aagctccggc tgcgcagtgc agttttgctg agaggttatg gttcggatat attctaaact
    14881 tgtaggttct ctgcaatggt gttgtgtgtc gtgttttgcc taaaaaccat tttgattgat
    14941 tgtgtgtcta tcgcagctgt gctcgttttc atccctactc tgaagggctc gggctttccc
    15001 cctcgcattc taaaaactgc tatatcgaat ctgatctgcc tgccactatt ccattatcag
    15061 tagaaaacgc ggtattaacc gttccactaa taagtgactg acttaccctt gagcgcgcat
    15121 ttagtgtctc gtctgtattt agtatgttgg gatcgacatt atcgtctgat atagacgcaa
    15181 cacaccaggt tccactggag gggttgccag aacaaatgcc tgcatagctt agtgattcgg
    15241 tgtttgtgcc ggacgcagat ggatatgtta tgatgaggtt gaattttgca aaattccaaa
    15301 gcttttccac tcccagacta ttgacggtaa agttgatcac gctagagccg gaagatgcat
    15361 caagtgagtc tatctcaatg tttgtcctca gaatggagtt ttctacttcg gaaatatcag
    15421 ttgaggccct tgttattgaa accgtttgat tgaccactcc tggtattgtc aataaaatgt
    15481 agactagggc aaatagcact ataccccctg aaactactat acttaggccc aattctcatt
    15541 cctcacaata attacgttga aagaacataa tctgaataaa cgccgtttgg agcagttatt
    15601 ctaaccacat atgtattgcc agccgctaga tcagagtccg ttatcttgat ttgtattgtg
    15661 tccatttttt ggatcattgt tggcggggtt gaaggctcaa aacgccatgt gtcttgcccg
    15721 cccgattcgt catatgcaac cctttgagcg gatcctgttt cgccatagta gacatccatc
    15781 gaagttgggg ccgttatcgg atcaattccg atgtttttta cccatacctc caaggtttca
    15841 ggagaagcgg tgctgttgta cattgcatga attactttga gttttgttag catttcgttt
    15901 ttttggcttt ccgatgtagc agtaaaggtg gactcaaagg tgcctacctg cgacatgata
    15961 attcccgcca tagtcgtggc aactacaata gaggcgatga tgaggattcc ttctgtcatt
    16021 attcctgcgc ccatctttaa tcactccctg tgttctgacg acgcggtcgt ttttgtgcac
    16081 taagccttgc ataataggcg tctgcttctt tgtcattaag accaacaatt tttgcaaatc
    16141 ggtacaactg cgatattgat tcttcaactg acatgccgga atttttgagc atgtcgatta
    16201 tgttgtagat ggtgtgttca tcttctggct ttaatcccat tagcttgcac tgctctacaa
    16261 gcaaatcgat gcagtcatca cctttttcct caaacatctc gcctactagc tcaattattg
    16321 ccatcatgtt tgcaactgag agacttccgt taaacaatgg cttcatgaca gactccattg
    16381 gattgttagt gtctgatccc tttacgaggt tatccatctc acttcccttt actatggcag
    16441 aaggtgacgg gggattttgt accgatgttg tggtgttgct tcctgctgct tgcataatga
    16501 ttggcatcgg gttgttttgg ttttgcgtat taggcgtgac attctgtgat tggttgtttg
    16561 catagttctg ggccatctgc tgggtttgaa gaattccttg tcttagtggc agggaaggat
    16621 cagacaggtt agataggtct agtgcttcaa agtattttct cataaagtta agtgggtttg
    16681 agatttctgc ttggaatgcc ttcatgtcag ttagtgttcc ttcaaatgat tcagttagtg
    16741 tatcgacact ttcttttgtg atcttcatgt cttccttgat cttgttaaga tcctttttga
    16801 aggtggtaag atctttttct actggttcaa acttttggtc gattagctca cgtattctac
    16861 tttcatcgaa tgccggtgta tctgcagcga cagttgtatc actgtttaca gtatcactag
    16921 tagcatcgga gccaccagca agcagcgcat caagctctgc attgctgcca ccaacttttg
    16981 atatgtctat gttttctgat tgtgcttcag atgttgctga ctgcgcaggt tgtgactcgt
    17041 ctccaattat ctgagtcagt agtggatctt cttcagatac tgctgcttgg ggctttgggg
    17101 ctgaattgcc acctagaaat tttggtaatg ccaccaatac agaagctatg gcacttactg
    17161 gaattacaaa ataatatagc gagtaattaa tatcaaaaag gtattctgga aagacttcgt
    17221 ataagactag tccatacatt ccaattacac tgcctgctac aaacattcga aacgtttgcg
    17281 tgaggctact cagcggtttt gtcaagaaag aaaaaaggtt gcttggagcg atcatagaaa
    17341 cactccagtg gtttatccga ggtctacaac ttctgtggta attgatggca cctgtctttc
    17401 aacagttagt gctgcaccag atggaactag tacttctgca cgtaccttgt cgagggcact
    17461 tggtctgtca tcggcagcat atactattgc caggacagca tgttcacctt ggtctagaat
    17521 gtcgttgttg ttactgttta cagtccagta gatgaatgcc tttgtttcag aaggccagct
    17581 gcttccatca ctttcgaatg gattgtcatt aatgaaggct tcctgctttg ctgtctcagc
    17641 tgcttctgat aattgcagcc attgtccgtc tgctgttccg tcaccatcat cttgtgcggt
    17701 agtggtaagt gttcctttgt agatgttgtc gtagctaagt gtgttactta ggtattttac
    17761 tgctgcagtg gtattagcca agttaacaga ttcgccacca gatgcgatct tgattgggaa
    17821 tgctgttacg ttgagttttg cggcagatac atgaccggaa ccaaagacct tgccggaaac
    17881 ctctaggctg ctacttgctt cacctaagcc tgagactatt gtagtctttg ctttctgtgt
    17941 tgtagagaaa cccatgttga gaacgacaaa agctaaggct gctgctacaa ttacaaatgc
    18001 aatcataaca attgcagact cgacgcctat gact
//
LOCUS       DASWKH010000090         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3207682, whole genome shotgun sequence.
ACCESSION   DASWKH010000090 DASWKH010000000
VERSION     DASWKH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207682"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGA92_07005"
     CDS             <1..822
                     /locus_tag="VGA92_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX9846198.1"
                     /translation="RMTALARQIQKFKPDLTISFCSPEAARISFGLGIRHIAFCDSPH
                     AEAVMRLSVPLIQKLLIPWIIPKNEFTKYGIDVKNIIPYKAIDASVIVKNTSSKKTGL
                     RQKGSKKNILIRVEEAQAAYVQKKQSQILPIIKEIAKRFPNESVLVLPRYRSQIVMLR
                     QTLGSRVKILGKVVVGKTLLQNTDVFVGSGGTMTAEAALLGIPTISYDAVPNLVQDYL
                     VRKKLVIRESNPKKIVSIIEKMLRGDNKYLRANAKKTLASMEDPLKKLIYAIKMR"
     gene            875..947
                     /locus_tag="VGA92_07010"
     tRNA            875..947
                     /locus_tag="VGA92_07010"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:909..911,aa:Gln,seq:ttg)
     gene            complement(956..1717)
                     /locus_tag="VGA92_07015"
     CDS             complement(956..1717)
                     /locus_tag="VGA92_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9846199.1"
                     /translation="MHPTIRLVNRNLTISLNPGFLVWQVIFPLVYIFVAGFAYTTLID
                     SVPFGNTSLDYPAFIASGMIGFNIMNSTLVSGIIIWNDRRHGMFEQILVGPFTRRNYI
                     LSNVYTIGIVGLISAAIITAIGFPLFFKSVEFNLLTIPFLVFASIVGSILFGSIASVI
                     STRLKSSEGFNVIINTVFLFFAFVSTAFYPAEGAPEPLATAFYLNPLTYLVDIIRAGI
                     FGTITEFVILEMAVLIVAATSLFIIAIKLLTKLDL"
     gene            complement(1701..2642)
                     /locus_tag="VGA92_07020"
     CDS             complement(1701..2642)
                     /locus_tag="VGA92_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9846200.1"
                     /translation="MSSILARSLSKSYGSVLAVDGIDLDVRSGHVFGFLGPNGAGKST
                     TIKLLTTLIPPTNGTIEILGINANKNPLEVRKRIGVVLQQPSYEPNLSVENSLEKYGM
                     MWNIDRKTRKERAAELIDVFELGEIRKRKNEDLSIGQRRRVQVAREFMHEMDLLFLDE
                     PTVGLDPGARRKLLDFLKNKAKTGLTIFYTTHVLTEAEYLCDEIAVIDKGKILAVDTP
                     DQLKNTFGQEKTIKIHLSDRHQKLAELLSEIGDCIIDFNTGTDIIIQSSKSEIVLSKI
                     LQILIENNIEVEDLSAVPTTLEEIFLKIVRNSDAPNN"
     gene            complement(2751..2906)
                     /locus_tag="VGA92_07025"
                     /pseudo
     CDS             complement(2751..2906)
                     /locus_tag="VGA92_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          940 a          614 c          589 g          849 t
ORIGIN      
        1 agaatgactg cactggccag acagattcag aaattcaaac cagaccttac aattagcttc
       61 tgctcacctg aagcggcaag gatttccttt ggattgggaa taagacacat tgctttctgc
      121 gactcaccac acgcagaggc agtaatgcgc ctttccgttc cacttatcca aaaactgcta
      181 attccttgga taataccaaa aaacgaattt acaaaatacg gcatagatgt aaaaaacatc
      241 ataccataca aggcaattga tgcttccgta attgtaaaaa atactagctc taaaaaaact
      301 ggattgcgac aaaaaggcag taaaaagaat atcctaataa gagttgaaga agcccaagcc
      361 gcttacgtgc aaaaaaaaca aagccaaatt ttgccaatca taaaagaaat agctaaaaga
      421 tttcctaatg agagtgtctt ggtattgcca cgatacagat cacagatcgt tatgctaagg
      481 caaactttag gatccagagt aaaaattcta ggcaaagtgg tggttggcaa gaccttgtta
      541 caaaataccg atgtttttgt aggctctggc ggaacaatga cagcagaagc tgccttgctt
      601 ggaattccga cgatttcgta cgatgccgta cccaacttgg ttcaagatta tcttgtaaga
      661 aagaaattag tcatacgaga gtcaaaccca aagaagatcg tttcaattat tgagaaaatg
      721 ttacgcggtg ataacaaata cttgcgagca aatgcaaaaa aaacattggc atcaatggag
      781 gatccgctaa aaaaactcat ttatgcaatc aaaatgagat agtcaacaaa cagaaaatat
      841 caataatagg agtttttgaa ggtggttaac aggaagcccg gtagtgtagc ggtcaagcat
      901 aggggccttt ggagcccttg accccagttc gaatctgggc cgggctactt tgattttaca
      961 gatcaagttt tgtgagaagc ttaattgcaa taatgaacag gcttgtagcg gctactatga
     1021 gcactgccat ctccagtata acaaattcag taatggttcc aaagattccc gccctaatta
     1081 tgtcgactaa atacgtaagc gggttcagat aaaaggcagt agcaagtggc tctggagcac
     1141 cctctgctgg gtaaaatgcc gtgcttacaa atgcaaaaaa caggaatacc gtattaataa
     1201 tgacgttgaa tccctcactt gacttgagcc gggttgagat tacagaagca attgatccaa
     1261 acaaaataga gccgacaata gatgcaaaaa ccagaaacgg aattgtcaat aaattaaatt
     1321 ccactgattt gaaaaacaat ggaaagccaa ttgctgtaat tattgcggcg ctaattagcc
     1381 ccacaattcc aatcgtgtag acattgctta gtatgtagtt gcgtcgagta aatgggccaa
     1441 ctaggatctg ctcaaacatt ccatgccttc tatcgttcca tatgatgatt ccagagacta
     1501 gtgtgctgtt cataatgttg aatccgatca ttccagatgc aataaatgcc ggatagtcca
     1561 gacttgtatt tccaaatgga accgaatcta tcagtgtcgt atacgcaaag cccgcaacaa
     1621 agatgtaaac tagtgggaaa atgacctgcc agacaagaaa gcctggattt aatgaaatgg
     1681 taagattcct atttacaagt ctaattgttg ggtgcatctg aattcctcac tatttttaaa
     1741 aagatctcct caagtgtagt tggcacggca gacaggtctt ctacttcgat gttgttttca
     1801 attaatattt gaagaatttt ggaaagcacg atttccgatt tacttgattg aattatgatg
     1861 tctgttcccg tgttgaaatc aattatacag tcaccaattt cagagagcag ctcagctagt
     1921 ttttgatgcc tgtctgatag atgaatcttg attgtttttt cttgtccaaa cgtatttttt
     1981 agctgatctg gggtgtcaac tgccagaatt ttacccttgt ctattacagc aatttcatca
     2041 caaaggtatt cagcctcagt caaaacatgt gtagtataaa agatagtaag gcctgttttt
     2101 gccttgtttt ttaaaaaatc aagcagcttt cttcttgcgc caggatccaa accaaccgta
     2161 ggctcgtcta aaaacaagag atccatctcg tgcatgaact cacgcgctac ctgaactcgt
     2221 cgtctctggc caattgagag gtcctcattt tttcgtttcc taatttcccc aagctcaaat
     2281 acatcaataa gctcagctgc gcgttccttt cttgttttgc gatcaatgtt ccacatcatt
     2341 ccatatttct caagtgagtt ttctacagaa aggtttggct cgtagcttgg ctgttgaagt
     2401 acgacgccga ttctttttct gacctcaagt gggttcttgt ttgcatttat tcccaagatt
     2461 tcaatcgtgc cgtttgttgg cggaattagt gttgtgagga gcttgatcgt agtagatttt
     2521 cctgcaccgt ttggccccaa aaagccaaag acgtggcctg atcgcacatc aaggtctatg
     2581 ccatctactg ccaacacaga tccgtatgat ttggatagcg atcttgcgag tatgcttgac
     2641 acaaatcttt ggatttagtt ctctaattta gactcatact gaaattttta tcgaaatagg
     2701 gcgagaagac caccggtttt aaccggtgga taaatcgcct actctttctg ctcacgaata
     2761 ctgatcaatg aatctaaaaa atccgcaata ccgccaacta gaatcttttt gcggtatttt
     2821 ggacaccaca caaaacgata gttgatattg tatgcagcag atctgcaacg tcttttctcc
     2881 acaattgtta aattagtgtc gtgcatcaat taaatccgtg gtaacgagaa cctacaaatt
     2941 tcgattatac ccggatcaca gccaatacaa aaaactccat aacactcttg gt
//
LOCUS       DASWKH010000091         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2827087, whole genome shotgun sequence.
ACCESSION   DASWKH010000091 DASWKH010000000
VERSION     DASWKH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827087"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /locus_tag="VGA92_07030"
     CDS             complement(<1..1992)
                     /locus_tag="VGA92_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX9846201.1"
                     /translation="MSVKCIPKTTVIALTLASAFFLIGANMEYSSAQYMQMVSPYMVS
                     FTVDKSTVTAGQTITFSGIVALAKTGTVTITILDSQSNVVLTEQSIVTNELFSHALST
                     TNLNPGTYTAKAQYGDSSKQLTFKVTTSGTDLSIQIIPSKNNPATIKPGTILSYTIKA
                     INNGPNPSTTNVVASISTSSGLLFYQQTSECITINKQIRCFYDASIPAQSSASKVLGI
                     QVTSSAAQSQTITAQVSTNGIETNPNNNMATLNVSIFVDDDNDGIPNSSDNCPSVPNP
                     SQDDSDKDGVGDECDSKPCFGSSLEVDLKSYTNQDTIYLTVIAPDANSNTKTKDTINV
                     VASHQTTSKNLSVLETGSDTGVFTTSFKASDIIPTGSGAITITYFDGCKNQDYQIQAN
                     VQGQQQTVSGSDFELQFLSDLPQNVNSDDIFTFQILYRNLGPEMAQSYRTVVEISPGL
                     RFDENYFSATRTYQGSKAFSNLGNLVPYVDSTPQYRIIVNNAATGTQFIKVIVDNVNE
                     LDPNLSNNYVEHFFQVEAQQQIQQPQDQDGDGIYDTNDSCITQPETYNGYEDTDGCPD
                     DVQTLDLNEPPAHKEDTITDTDGDGIYDTNDSCITQPETYNGYEDLDGCPDDVKILDP
                     NEQIPHKEDTITDTDGDGIYDTNDSCITQPETYNGYEDLD"
     gene            complement(2046..2870)
                     /locus_tag="VGA92_07035"
     CDS             complement(2046..2870)
                     /locus_tag="VGA92_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9846202.1"
                     /translation="MSDKIAAEFLELSSEQRLGIIQMLSSKKSKLSEIAKKLDSTNSE
                     AHRNLSRLTKAQIISKDGDGNYHLTAYGNMLCLMIPSLRFFGDNKKFFSNHGFGSLPA
                     NYIQAMGSLADSKRISGYVRVMECWKNIYQNATKYIYNILNEVSYDSETINIVKDKLK
                     SGMAINTVFIENAVISDNRKDILENTELGKLIKNSHLERRFSKDASIVMVLNEKQSSV
                     AFPATDGKIDISEVFYSDSPEFHDWCLSLFRHNWELSTAFFDEKLKNQSGEHSNHI"
     gene            3017..3349
                     /locus_tag="VGA92_07040"
     CDS             3017..3349
                     /locus_tag="VGA92_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846203.1"
                     /translation="MGLLTSNMSKYYLKRCSREGRTLRITISVEGVIEEKLKEIQRYL
                     FSYTGEEWSMSKVINMILLGGILADKRLGIQDWYAIKKFTEGSYTAISDVEVANYIIN
                     IAALSQTV"
     gene            complement(3333..4550)
                     /locus_tag="VGA92_07045"
     CDS             complement(3333..4550)
                     /locus_tag="VGA92_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846204.1"
                     /translation="MIRQNRPDLIYANFFFTDIVGLSNPNMSTTTQFKKLEVLNKIIQ
                     ESNAYKNTPKTDMITFPTGDGMCIGFTKDQETPMLLAFELHKKLYEYNKGKIPSEIIQ
                     IRIGLAGGNCFTFKDINENINVWGPGIIVTKRIMDIGDANHILLSQRFAEDLRELSDE
                     YMKIIKPIQDYAFKHGITMLVYSAYGQGFGNPIPPKQEQLESMMQNEIIKLQNTALYP
                     CVDVTLELANPKSMSFHHKRTYQIRNISDEPIRYVLHGIATDVEKNDVSDLNIKTYDE
                     SGEEMRISSINLNQPFCKEFSTVFKRPILKGQESRAYTLEYDVEEPEGFFENNFAIDC
                     KKFILRFAYNEGDLQKHPQLYYLNQETEEKTPDEAVPIVSKNDSKTTLTWEKENLVKG
                     QTLRLEWQARQSD"
BASE COUNT         1128 a          862 c         1060 g         1594 t
ORIGIN      
        1 atcaagatcc tcatatccgt tgtatgtctc cggttgtgtt atgcaggaat catttgtgtc
       61 gtagatgccg tcaccgtcgg tgtcggtgat tgtgtcttct ttgtgtggta tctgttcatt
      121 agggtccaat atttttacat catccggaca gccatcaaga tcctcatatc cgttgtatgt
      181 ctccggttgt gttatgcagg aatcatttgt gtcgtagatg ccgtcaccgt cggtgtcggt
      241 gattgtgtct tctttgtgtg caggcggttc attcaaatcc aatgtttgca catcgtctgg
      301 gcagccatct gtatcctcgt atccgttgta tgtctccggt tgtgttatgc aggaatcatt
      361 tgtgtcgtaa atgccgtcac cgtcctggtc ttgtggttgt tggatttgtt gttgagcttc
      421 aacttggaaa aaatgttcta catagttgtt tgataaatta ggatcaagct cgtttacatt
      481 atctacgatt accttgatga actgcgttcc agtagcagcg ttattgacta tgattctgta
      541 ttgcggtgtg gaatcaacat atggtaccaa attgcccaaa tttgaaaatg ctttgcttcc
      601 ctgataggta cgcgttgctg aaaaatagtt ttcatcaaac ctcaaccctg gtgaaatctc
      661 tactacggtc ctgtagcttt gtgccatctc aggcccaaga tttctgtaca aaatttgaaa
      721 cgtaaagata tcatcagagt ttacgttttg tggcaggtct gagagaaatt gcagctcaaa
      781 atcagagccg gacacggttt gctgctggcc ttgaacattt gcttgtattt ggtagtcctg
      841 gtttttgcat ccatcaaaat aggttatggt gatggcgcca gagcctgttg gaatgatatc
      901 gcttgcctta aaagacgtgg taaatactcc agtgtcagag ccggtttcca atacggagag
      961 attctttgag gttgtctgat ggcttgccac gacgttgatt gtatcttttg tttttgtgtt
     1021 agagtttgcg tctggggcaa ttacggtaag gtagattgtg tcttggtttg tataggattt
     1081 cagatccact tccagtgagg agccaaagca gggcttggag tcacactcat caccaactcc
     1141 gtctttgtct gagtcatctt gggatgggtt tggcacgctt gggcagttgt ctgaggagtt
     1201 tggtatccca tcattgtcat cgtctacaaa tattgaaacg ttcagtgtgg ccatgttgtt
     1261 gtttgggttg gtttctattc cgtttgttga gacttgtgca gttatcgttt ggctttgggc
     1321 tgcactgctg gtcacctgta ttcctagaac cttgctagcg cttgattgtg caggtattga
     1381 ggcgtcgtaa aaacaccgta tttgcttgtt aattgttatg cactctgaag tctgttggta
     1441 aaaaagaagc cctgaagaag tggaaataga tgcgaccacg tttgtagtag atgggtttgg
     1501 tccgttgtta attgccttta ttgtgtagct tagaattgtg ccaggcttaa tcgtggcggg
     1561 attatttttg gatgggatta tctggattga aagatctgtc ccgcttgtag taaccttgaa
     1621 cgttagttgc ttgctagaat ctccatactg tgcttttgca gtgtatgttc caggatttag
     1681 atttgttgtg gacagtgcgt gtgaaaaaag ttcgtttgtt actatggatt gctctgtcaa
     1741 gacgacatta gattgtgaat ccaaaattgt gatggtaact gttccggtct ttgctagggc
     1801 gacaattcca gaaaaggtaa tcgtttggcc tgcagttact gttgatttgt ctacagtaaa
     1861 tgataccatg tatggtgaaa ccatttgcat gtattgagca gaagagtatt ccatgttggc
     1921 gccaatcaag aaaaatgccg atgccagtgt cagtgcaatg actgtagttt ttggtataca
     1981 ttttacagac atgctaaaaa tttgtgaaaa gatcatacat tagataatag actactgatt
     2041 tttatttaaa tatggtttga atgttcacca ctttggtttt ttagcttttc atcaaaaaat
     2101 gcggtagaaa gctcccagtt gtgtctgaag aggctcaggc accaatcatg aaattctggc
     2161 gagtcactgt aaaaaacctc gcttatgtcg attttgccgt ctgttgccgg aaatgcgaca
     2221 cttgactgtt tttcgtttaa gaccatgaca atcgaggcat ctttgctaaa tcttcgctca
     2281 agatggctgt tttttatcaa tttccctagt tccgtatttt ccaaaatatc ttttctgtta
     2341 tcagaaatga ctgcgttctc gatgaatacg gtgttaatgg ccattccaga ctttagcttg
     2401 tcttttacaa tattgattgt ctcagaatca tatgatacct cattgagtat gttgtagatg
     2461 tattttgtag cgttttggta aatgtttttc cagcattcca ttacccttac gtatccagat
     2521 atgcgctttg aatctgcaag cgagcccatt gcctgaatgt agtttgcagg taggcttcca
     2581 aagccatgat ttgagaaaaa ctttttgtta tctccaaaaa aacgcaaaga tggaatcatc
     2641 aggcaaagca tgtttccgta tgctgtaaga tggtagtttc catcgccgtc ctttgagatg
     2701 atctgtgcct ttgtaagccg gcttaggttt ctatgtgctt ccgaattcgt agagtcaagt
     2761 ttctttgcaa tttctgagag ttttgacttc tttgatgaga gcatctgaat aataccaagc
     2821 cgctgctcac tcgatagttc caaaaactct gctgcaattt tatcagacaa cacatatgtc
     2881 cttaatgaac tgaattaaaa acttcaaggt cattagcaga aagtttgtat tttgtttgta
     2941 ttcaaacaaa atgatcctat atttgttcta aaaccagctg aaattttcgc attaaagctg
     3001 aataggagat atgtctttgg gcctgctaac tagtaatatg agcaagtatt acctgaaacg
     3061 ttgttcaagg gagggcagaa ccctcagaat aactatatcc gtcgaaggag taattgagga
     3121 aaagctcaaa gaaatccaga ggtatctttt ttcgtatacc ggcgaggagt ggagcatgtc
     3181 aaaggtcata aacatgatac tccttggtgg aatacttgcc gacaaaaggc taggaataca
     3241 ggactggtac gcaataaaga aattcaccga gggtagctac acagccatat ccgacgtaga
     3301 agtcgcaaac tatatcataa acattgccgc cctcagtcag actgtctagc ctgccattct
     3361 agtcggaggg tctgtccttt gactagattt tctttttccc atgtaagcgt agtctttgag
     3421 tcgttttttg aaactattgg tactgcctca tcgggagttt tttcttcggt ttcctgattg
     3481 aggtaataaa gctggggatg tttttgcagg tcgccttcgt tgtaggcaaa tcttaggatg
     3541 aattttttgc agtctatggc aaaattgttc tcaaagaatc cttccggctc ttcgacatcg
     3601 tattctagtg tgtaagccct gctttcttgg cctttgagga ttggcctttt gaaaaccgtg
     3661 ctaaactcct tgcagaacgg ctggttcaga ttgatgcttg atattctcat ttcctctcct
     3721 gactcgtcat atgtcttgat gtttagatca gagacgtcgt ttttttcaac atctgttgcg
     3781 attccgtgca gaacgtacct tattggctca tcggagatgt ttctgatctg atatgttctt
     3841 ttgtggtgga atgacattga ttttgggtta gccagctcaa gtgtcacgtc aacgcacgga
     3901 tacagggcgg tgttttgaag ttttattatc tcattttgca tcatgctttc taattgttct
     3961 tgttttggtg gaatgggatt tccaaagccc tggccatacg cagaatagac aagcattgtg
     4021 attccgtgct tgaaggcgta gtcctggatt ggctttataa ttttcatgta ctcgtcggat
     4081 agctctctga ggtcttctgc aaatctctga ctcaaaagta tgtggtttgc gtccccaata
     4141 tccattattc tttttgtgac aataattccc ggtccccaga cgttgatgtt ttcgttgatg
     4201 tccttgaagg taaagcagtt tcccccggca agcccgattc ttatctggat tatttcggat
     4261 gggatctttc ccttgttgta ttcgtatagt tttttgtgca gctcaaacgc caagagcatt
     4321 ggagtttcct gatcttttgt aaatccgatg cacattccgt ctcccgtagg aaaggttatc
     4381 atgtcagttt ttggcgtgtt tttgtaggca ttagattctt ggatgatctt gtttagaacc
     4441 tctagttttt tgaactgggt agtagtagac atgtttgggt ttgagaggcc cacaatgtct
     4501 gtgaaaaaga aattggcata aattaggtcg gggcggtttt gtcggatcat cacatattga
     4561 tagtttcatt ataaataaaa attcagtgcc gaaaaagcca gtgatagatt attcgtgttt
     4621 taactgaatt tatgataatt taga
//
LOCUS       DASWKH010000092        27568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3548429, whole genome shotgun sequence.
ACCESSION   DASWKH010000092 DASWKH010000000
VERSION     DASWKH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 27568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27568
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548429"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1393
                     /locus_tag="VGA92_07050"
     CDS             386..1393
                     /locus_tag="VGA92_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX9846205.1"
                     /translation="MWKYVDIGEGEKIFHKVDDCILSKKTKYQLLEIIKTKDFGNVLF
                     LDGDPQSSEVEEYIFNECLVHPAMITQSKPKTVFIGGGGTGLTLREVLRHSAVERVDI
                     VDIDKEVVDICKKFYNYAAESFSDPRTKLYHEDARQFLSNTDSKYDCIFLDTTMPHHD
                     DIAAPLYRKEFFELASQKLKPDGAFITAANSADFRYSTRFASIVKTLQQVFAFVKPYT
                     AYTPLFGQEWAFAFASNTIDPTKLKPHEVNEKLVETGCNGLNFYDGLTHQRIFSIDKK
                     MRKVLKEEGTILTDSNQSQDEIFVPEISESPDFENFVNMIDLHQYSSVIKLASTPHKT
                     F"
     gene            complement(1378..2157)
                     /locus_tag="VGA92_07055"
     CDS             complement(1378..2157)
                     /locus_tag="VGA92_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846206.1"
                     /translation="MSKSNPTPEHIGSKIDYKILVIIFCLGLVYHVINISIKDITEEF
                     NAIDIIELSLQSAVIVSAFIISKLYWPSKVFGKAYFALGIAFGMWFIAEVLWQIYENI
                     LFIEPYPSFADIFYFAFYPFALYHMITNMKGFEVKITKRTVVWMVLIPVFILTVYSYI
                     AIEEWHDIGFDYYYTMIFVSIASVSASFALLGTQMFKQSALAVVWSLLAVGLFLHIFA
                     DIWYYYLEIFGQYTDTHVVNVFWQAGWMVIIYALYKHQKVL"
     gene            2339..3298
                     /locus_tag="VGA92_07060"
     CDS             2339..3298
                     /locus_tag="VGA92_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9846207.1"
                     /translation="MINLLYLVNLSSVFSRFPRIKVATTLGFTVAILSAALSALPDVI
                     TKPIVDPGTDGILPTEPLLVVLIMYLMTGIFFTPLAKLQKPRVKIKRSSWPILIIYGV
                     ASASSTLVFSFGLQETSATNASILANSETVFTILIGMLICKECLAKKELLPLTLIVVG
                     AIFLPIGSDVYNQSFAFSEFVFGDILILISGFIYCLCTFIAKRASMIKTTSVVQIMSF
                     SGAAFIFILMLIFQTDFTIDHSNFSLLSFVGIAGIGGSVLFFVMAVRLIGAVRTILIF
                     SSTTIFGVFYSGVYLLEPISLFHISSVALVVLGLHQLRYRLAA"
     gene            3445..4053
                     /locus_tag="VGA92_07065"
     CDS             3445..4053
                     /locus_tag="VGA92_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9846208.1"
                     /translation="MVGIDRLLLKSLDTTIRENLGDRTIKKIENRLSEKYGISLTQSI
                     EEFQKLDIVLREFFGPSTDGLEKKFLRSVCEIKLMDGENWVLIENPELTRTILESFGD
                     DDKKKILSALNGNSLIVSQILEKCNIPQTSGYRKVNSLIKEGLLVPSGYIAAPDGKKI
                     TKYRSIFDNIKIDILKDKIVITLHLPKEDFSSSSILSVCTQQ"
     gene            4098..5087
                     /locus_tag="VGA92_07070"
     CDS             4098..5087
                     /locus_tag="VGA92_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9846209.1"
                     /translation="MAWDPLRTISKNKNSFQLGYVLILISAVLASLVHVLAKPLVDNQ
                     VGFEIHPVTLAACIYIINGAFFTPFTRKSKPIASLGRRNLVLLAVIGISEVIALITYF
                     FGLKDSTAANASIFSNGEIVFSLLIVMVIFKENLRKKEIGPFVMMLLGMMVLPVAYDF
                     YSHGMVISEIVIGDILIIASGTFYALDVMLCRYLSDKIDSKRITQITSLVSGTIAVAA
                     MIVFHIPFNMDLLSLGPIALFAIGGTGLATMLFLVSLRLIGGVRTILLFSTNSVLGVV
                     FAALILSETITVMNMISVALTFGGICLLRNRLGGSERTENTQKQKSDLVRHSS"
     gene            5483..6445
                     /locus_tag="VGA92_07075"
     CDS             5483..6445
                     /locus_tag="VGA92_07075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9846210.1"
                     /translation="MTKNGIVCFTNGRTVNLDPRFATPQSVNFVSHAHLDHLPNKKEG
                     LILATRETREIATLRGHDLLNHVESLDDFNLHDSGHILGARGLHFDDVFYTGDVCTRD
                     RGFLCGASIPKCKILITESTFGRPEFVFPKVDEVVKRVNEIIAELYHKGKPVLLLGYQ
                     LGKAQTLSHLFGHWEPLYYHDSVKEMNDLHRRLGIQLKAGLGHTEAESSGLLDKKPWV
                     MVCPLMSENNPFIKQMKSKYGAVTIGFSGWATSPHMSYGRKNDYSVPLSDHCDYNELV
                     ELVKKTGAEKIYTVHGFVEEFANSLTKMGFDAQPLRENSLDDFT"
     gene            6534..6839
                     /locus_tag="VGA92_07080"
     CDS             6534..6839
                     /locus_tag="VGA92_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846211.1"
                     /translation="MSQTVQQVAGKELWVLVSQLKENADSAELEKIVPQVLELIDSWT
                     KNGKFIWSGPFGNETGGMAIFEGTQNEANQFAENYGKICSNVLNYYTYKWDILWGSK"
     gene            6894..7448
                     /locus_tag="VGA92_07085"
     CDS             6894..7448
                     /locus_tag="VGA92_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX9846212.1"
                     /translation="MSTASLKKDHDLIEKIVRAMEATLQLLKAGKTIPESILIPVIDF
                     SKNFTNVCHHGKEEESLFPALEKHGFPQNTGPIAVMLLEHEMTRQIAARMEESTKEYL
                     ESGRYEKLVTDISDYIGHVREHLWKENNRLFVMAEMRLQGQSEQINKNLGDVELQKLA
                     SLGRNRADYEKLVSDLEQNVSKIA"
     gene            complement(7486..8715)
                     /locus_tag="VGA92_07090"
     CDS             complement(7486..8715)
                     /locus_tag="VGA92_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9846213.1"
                     /translation="MTTADKFGIAFSIGFTVALIAIAMSLSAMSQSPAPVATTPPPVP
                     VPEPLSTPQPTQPTKTPVAGYDRLTSIQDPGIGHESHQLAVILAPSDKVYSGTLMYDA
                     SEPIQLVALTGPLAPGEDMGQPIWTPDGETKFALTLVDPKNSRGEWKFAGNALAVHTM
                     KTSPFIVDYKVDYEEMSASDTVKTGTIQSVQDPGIGHESHQLAVILAPSDDTYSGILA
                     YSASEPIQLVSLRGPLASGETAPAIWTPDGETKFALTLVDPKTSMGTWEFSGNALAVH
                     TMKTSPFTVSYSVSATATPAEPVSVEEPVMEAAPMSAEVSIPSGSSLPDCVETNECYI
                     PYLVTISVGGTVTWTNDDTAVHTVTSGTVTSGPDDIFDSSIVMAGKTFEHTFDDAGTY
                     DYYCTYHSWMTGQVVVE"
     gene            complement(8798..9457)
                     /locus_tag="VGA92_07095"
     CDS             complement(8798..9457)
                     /locus_tag="VGA92_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846214.1"
                     /translation="MTGSDSDKIAKKILFDPVDFKLTVAKHFLHKIPNKIPNKDLEHF
                     LCEVNVETFLFFCSSSVDIVKREINEKFKLFDKENVFYIHGVRKKLSDSGDQKKTKQI
                     IADYFTTPTHAKTTKSGWNAKQSSLWRLQILRNQATHGHILKKTSGGKALSFVYVVRE
                     YKKHRKTPYLLKELTKNPKKYFGEIFDDLCEFVKKTRKITAPKIQSQYHKKRIYDFIP
                     D"
     gene            complement(9500..9772)
                     /locus_tag="VGA92_07100"
     CDS             complement(9500..9772)
                     /locus_tag="VGA92_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846215.1"
                     /translation="MLINLLRYNSSASVQEPIRIFLYNYQIMSDNFWEMYKKAKSHED
                     VLECYYQFSKNQCTIIETLLSNLKLSLNEDHLKEDLHLMLKDAFTF"
     gene            complement(9936..10175)
                     /locus_tag="VGA92_07105"
     CDS             complement(9936..10175)
                     /locus_tag="VGA92_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9846216.1"
                     /translation="MPSAYVIIHCEPTETYYVIRNLKYIPEVKGADDVFGYYEIVCKV
                     VADTQRELEKIITAKIRKIKYIISTMTLTVVEGQG"
     gene            complement(10175..10429)
                     /locus_tag="VGA92_07110"
     CDS             complement(10175..10429)
                     /locus_tag="VGA92_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9846217.1"
                     /translation="MSIAYFLINAKLNHEGEIIDKAKSVLKNSSLQSYEVQGVFGIYD
                     IIVKMSATNDDILRKIVLEKLRKIENIESAITMMVNEEQE"
     gene            complement(10433..10672)
                     /locus_tag="VGA92_07115"
     CDS             complement(10433..10672)
                     /locus_tag="VGA92_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9846218.1"
                     /translation="MATAYVLINCELGSEQSVIDQIKPIEGIKEVRGVFGAYDVLTKI
                     ETSSAEKLREIITWKIRKIDRIRSTLTLMGIEGQE"
     gene            complement(10802..12391)
                     /gene="uvrC"
                     /locus_tag="VGA92_07120"
     CDS             complement(10802..12391)
                     /gene="uvrC"
                     /locus_tag="VGA92_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9846219.1"
                     /translation="MTLNIKKITIPSHPGIYLMKDDGGKIIYIGKAKNLKNRVRSYFL
                     KNQNYKTQKLVEKIKNIEFVLTDNESEAFLLESNMIKQYRPVFNIELKDQQRYTYLRL
                     TEDQYPRLVVARRTRTGKFLGGGKIYGPFTSGSSKLLTIGSLRKAFKIRICKRLPKKA
                     CLEYHIGNCEAPCEFKEAQKRYEWHVSDLESILRGRDLRAFIKKLESEMREASEHLQF
                     ERAREIRDTLQRLGSLKTRQKMETVSDSDEEYFGLKIIDQTAQIMTFKKINGVIRDSN
                     KFSFDLVADNSFSNFLFQYYTTNRIPPRIIVSQMPDNAKLLEGLFSKTVGFSVRITVP
                     NGGKRKEMLDLIMRNIELIESKGAEPGLVELKEILKIPQIPKVIECFDISNHGASFAV
                     GAMSRFVNGRPDKSGYRRFRIRTVVGQDDFAMINEIIKRRYFRLDNENSQMPDLILID
                     GGKGQLRSALSALQSLALSVPCASLAKENEEIFVPGKKEPVIISKDRKSLQILQFARD
                     EAHRFGVAYNRILRTPKAKFN"
     gene            complement(12388..15204)
                     /gene="uvrA"
                     /locus_tag="VGA92_07125"
     CDS             complement(12388..15204)
                     /gene="uvrA"
                     /locus_tag="VGA92_07125"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX9846220.1"
                     /translation="MHDKLKIRGARHHNLKNLNLDIPKNKLVVISGLSGSGKSTLAFD
                     TIYAEGQRRYVESLSAYARQFLEMMDKPDVDSIEGLSPAISIQQKTTSKNPRSTVGTT
                     TEIYDYLRLLYARIGIPHCIKCGRKISNQSIESICESIFKDFDDRQTLVLAPLIQRKK
                     GTYEKLFEQIKKDGYSRARVDGQIILLEDEIPRLDRQKWHHIEIVVDRLKASKAEKSR
                     LFEAIQTALKASKGSVLIVADKDEKVFSQNNACPHCGITIGEIEPRTFSFNSPFGSCK
                     ACHGLGIKMEFDPDLVIPDKTKSILDGAIVPWSGRFSSFRKQELRAVGKKFGFDLMTP
                     INKMRPEQLKVILHGTDQMIHYQYESQTTDSSWQYTNSFEGVLNNLHRVFMDTDSESK
                     RDWLKQFMRDSPCNVCNGKKLKPEALAVKINGMGISEVCDLSVDECYDFFQNITLTET
                     ERYIARDVLKEITERLEFLKNVGLNYLTLNRRSSTLSGGESQRIRLATQIGSNLTGVL
                     YVLDEPTIGLHQRDNARLIKTLVKLRNLGNTIIVVEHDEEVIKNSDWIVDLGPGAGIH
                     GGKVVFEGTVEQILNNHKSVTGDYLKDKILIKLQNKTRNQKGRITIRGAAENNLKNIT
                     VEVPLGLLVTVTGVSGSGKSTLVNEILLKTLSTHFYKTNERPGKHKSVDGMENIDKVI
                     AIDQSPIGRTPRSNPATYIGVFTPIRELFASTESAKERGYTPGQFSFNVADGRCFACE
                     GDGVKKIEMQFLSDVYVRCDECKGKRYNSETLSIHYKDKNVADILDMTVEEALEFFEN
                     IPKIRRILQTIYDVGLGYIKLGQSSTTLSGGEAQRVKLASELSKRDTGKTLYILDEPT
                     TGLHFADVQKLLDVLNRLVNLGNTVVVIEHNMDVIKNSDWIIDLGPEGGDKGGQVVAT
                     GTPEQVAANPKSYTGQYLKKILVN"
     gene            complement(15194..17143)
                     /gene="uvrB"
                     /locus_tag="VGA92_07130"
     CDS             complement(15194..17143)
                     /gene="uvrB"
                     /locus_tag="VGA92_07130"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9846221.1"
                     /translation="MQQVQTFQIVSEFEPTGDQPQAVTKLVDGVKKNKVQTLLGVTGS
                     GKTFTIANVVAQTGKNTLVISHNKTLAAQLYSELKQFFPKNNVGYFVSYYDYYQPESY
                     IAQTDTYIEKDTEINEKIEKMRLEATAMLLSGEPTIIVATVSCIYSLGSPKEWEEMAT
                     TVTANATYSRTELIKKFINARYERNDLEPVPGNFRVKGDTIDIVPAYSEDIIRISLFG
                     DQIEKIVLLDRVSLKEKRKLNSIKIFPAKHYLIADDVRQKAVKSIRDELKQRLTQLNE
                     LERQRLEMRTKFDLEMIEELGYCSGIENYSRHFDGRGPGEKAFCLLDFFGDDFLLVID
                     ESHATIPQLHGMHGGDYTRKKSLVDYGFRLPSAFDNRPLKFAEFESYIKNCIFVSATP
                     STYEKEISYQIAEQLVRPTGLIDPTVEVRPTKGQMDDLISEIQKRTKKNQRVLVTTLT
                     KRMAEDLADYLSKKEIRVRYLHSEIEGLQRTELIRQLRLGDFDVLVGINLLREGLDIP
                     EVALVAILDADKEGFLRNYTSLIQTFGRAARNADGTVILYADTITKSIRAAIDETGRR
                     RKKQIEYNKLHNITPRTIIKSIPEQVAALDDIKNKTLHDLGRESIEVEAQMKKYAEDL
                     DFEKAIECRDRLRRIQIELEKKNAR"
     gene            complement(17214..17846)
                     /locus_tag="VGA92_07135"
     CDS             complement(17214..17846)
                     /locus_tag="VGA92_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9846222.1"
                     /translation="MQLLGRLEIKSRQKIIQFLNEEHVGRICSIDKDGYPQVIPMNFV
                     FLNDAVYMHSHTRGEKLDNIQANSKVGFEVDRELEFLPSYFTHPTDASQADTLYISVV
                     IKGTGTIIEDRQEKTLALNGLMEKYQPEGQYEKITPDMEVVDEVAIIKVVPKTMHGKY
                     KIGQHMNTASRTELAKKIFERNSATAKETLKIMGFDVSGKELRLVDEPLW"
     gene            complement(18019..18423)
                     /locus_tag="VGA92_07140"
     CDS             complement(18019..18423)
                     /locus_tag="VGA92_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846223.1"
                     /translation="MKGDVLLQNIQVKGFVPATAGRNVKADKKKILDAIETKLGKNIN
                     NVRKRCAGKPLSINVTFFLQKDSAHAKKDLDSLSNVLLTILSKEIGTSKEAGLDIIND
                     DSIIYRYVFEKKIVDAEKDEGLAFSIYEWSET"
     gene            complement(18500..19009)
                     /locus_tag="VGA92_07145"
     CDS             complement(18500..19009)
                     /locus_tag="VGA92_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9846224.1"
                     /translation="MVTIQEAESYARAKNADIAFCKGIVFRLKSIGISDEQVLSTAFL
                     CQTKASFDEIYSRFGREIATSVASISKDFTLPRQRQEEQYIEQLQAAPWGSILIKLCE
                     ISANLKTIKESEMSKTKRGKILKQNIHHLNVLKKKIAENKSATPGVERLLDGINETLV
                     HFRQRPIKF"
     gene            complement(19039..20124)
                     /locus_tag="VGA92_07150"
     CDS             complement(19039..20124)
                     /locus_tag="VGA92_07150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9846225.1"
                     /translation="MLKFGIQNGLNVARAGFSEDQILTACLLADRTGYDSIWYMDHSN
                     VPQWSKSTVLDPWVMLSAIAATTQRVELGTCVTDAIRRHPSNIALASITLDRVSKGRG
                     TLGIGAGEAQNLKEFNISFENPVSKWEEQLQVIKLLYESSPEHRVSFNGKYYQLSDAC
                     LQAKPIRKPHPPIYMAAGGQRTLKMTGKYGDGWLPIGYTPELFEDHVKTIKDSMKEEG
                     RSKEDQENFQYALDIDVYFSEDAEESWAKMKEAVKVSLFKPEVLRVHNIKQIEGFDFK
                     KYFTEYSMSNQEWIVKMREAATTIPDAVARSSVAVGTPDDVIPVFERFLKAGVNHFVI
                     RFWGSNYFGSIDKFASKVMPYLKEKYK"
     gene            complement(20221..21165)
                     /locus_tag="VGA92_07155"
     CDS             complement(20221..21165)
                     /locus_tag="VGA92_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9846226.1"
                     /translation="MHTIRIPKIIHFGKDALSEAEYPKNALVVTTAPPPISTKWLGKM
                     GIKDYMLYDKVEPEPSIETVQQVIADFKSKNPSAIIGLGGGSSLDVAKYAGSEMKLEK
                     ILIPTTFGTGAEMTTYCVLKFEGKKKLLREDRFLADRAVVDAYFMDGTPEQIVKNSVC
                     DACAQATEGYDSKIGNEFTRTMCKQAFEVLYDAIINNKPENYPYGSMLSGIGFGNCST
                     TLGHALSYVFSNEGVPHGYSLSSCTTVAHKFNKSIFYDRFKEVIEKLKFDRLALKAPL
                     DQAADLVMTDRGHLDPNPRPVTKEDVIQCLKDIVEGRL"
     gene            complement(21192..22061)
                     /locus_tag="VGA92_07160"
     CDS             complement(21192..22061)
                     /locus_tag="VGA92_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX9846227.1"
                     /translation="MAKKPKAFKKICWANTSSFDDSDIVIVGIPDESGSHSYSDGTSK
                     APDTIRRISNERDIYIEGKLRCLALPTKGPSNTQVYDYGNIKKQQIPEVFDRISFASK
                     IPISIGGDHSNTAKILKALAKKHAPISLAYFDAHPDFVSHTKNYYGSIITDSLDYLDI
                     KSSIVVGARTPEQEEVDNIKKHGLRVITPFDIIEKGVKQITKVILDTVKKNTYVSLDM
                     DCLDPAYAPGVSVPVPIGLESQDVTYMIKKIVQRGIIGFDVMEVCPNNDLNDVTSHLA
                     SRLIGEVVSSCKI"
     gene            22178..23446
                     /locus_tag="VGA92_07165"
     CDS             22178..23446
                     /locus_tag="VGA92_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HEX9846228.1"
                     /translation="MIKNKKQIYNAKTKDAIQILEAGLDAAKPERILFRFVRKGKLDV
                     NKKSYLLSKYRKIHLVAVGKAADSMAKLVHDKIHVDSGIIVIPQNYNSMLSAKNFKIF
                     RAGHPIPNKTSVIAAKSIINLVEGAKKEDLVIFLVSGGASALTCLPDGIALGQKQQLT
                     QILLRCGASINEVNALRKHLSQIKGGRLLEHLHCAAVSFVMSDVVGDDLSSIASGLTY
                     CDKTTYSECLGIIKKYGIEKQISAQALRQLKLGAKGKFAETPKRPKIPNIVVASNQDC
                     LDAMKNTATKLGYRTKIFAPLTGSTNSAAKKILQNFSFRKKSCLIFGGETTVKVIGDG
                     RGGRSQDLVLQILNRMMENTVVAAMGTDGIDGNTKDAGAIFYMIPRKNKVRDYLKNND
                     SNSFFKKYGGLITTGPTHTNLLDIGLILKS"
     gene            complement(23435..23821)
                     /locus_tag="VGA92_07170"
     CDS             complement(23435..23821)
                     /locus_tag="VGA92_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846229.1"
                     /translation="MEHTLHSDGNAKRICWTIKTQDIINEQFREQAEIFLDQVTNLQS
                     KYIALHVGLFWSIGVFIIKNGDTVKIMLDSDEMLAHLSSDDKSQDAFAENRKGFINQL
                     ASQRNLKLQYEKIDPTQNLASKMLRF"
     gene            23904..24110
                     /locus_tag="VGA92_07175"
     CDS             23904..24110
                     /locus_tag="VGA92_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846230.1"
                     /translation="MQKSFVFFTRMVDELVEFSDSDPELAEGIRWLDMQAQKKGISFY
                     EMVFQVLYKHDINTRAQQWLNSRN"
     gene            complement(24107..24256)
                     /locus_tag="VGA92_07180"
     CDS             complement(24107..24256)
                     /locus_tag="VGA92_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846231.1"
                     /translation="MVWIKWDDYGKKPKRKKLAKKKKPRIEPPVKIIPGKHKVEFKRA
                     SELSD"
     gene            complement(24287..25450)
                     /locus_tag="VGA92_07185"
     CDS             complement(24287..25450)
                     /locus_tag="VGA92_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX9846232.1"
                     /translation="MDTKNIGLRDIEVADTKISDIDGEHGKLIYRGYDILDLTKNSTF
                     EETAYLLLNDDLPTRAQLDAFKQELIGARDMPRQMQSNMRNWRKNADPMVMLQAFVVA
                     LAGYYDDKGANEEEINHQSAINLIAKIPTTIASWERVRTQKDIVDPDPRLSHSANFLH
                     MLVGESPDAETERIFDICLILHADHTFNASTFAAREVASTGAHMYSAVSAAIGALSGP
                     LHGGANFEVMKMLLEIKNADNAETWVKEKLARGEKIMGMGHAVYRTYDPRAQVLKELS
                     KRLAKKTGAPWFDITERVEETTIREMKKQKGIDIYPNVDLYSASLYYMLKIPSDLNTP
                     IFAISRVAGWTAHVIEEKFATAAPKPVLYRPKATYVGKYCGPQGCEYKPIDLR"
     gene            25555..25752
                     /locus_tag="VGA92_07190"
     CDS             25555..25752
                     /locus_tag="VGA92_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846233.1"
                     /translation="MSQAERDFIKQIDDLILGASQEDLSKIAELDKKTQLDGKTFYDV
                     YLALSDKDRKQIHDFVSIKKR"
     gene            25853..26806
                     /locus_tag="VGA92_07195"
     CDS             25853..26806
                     /locus_tag="VGA92_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX9846234.1"
                     /translation="MEDADGVSSAALIRQVFGGDTILVDYPGMMPAIEALAKDEKLGA
                     LYICDLGISKTNQDKFVEVLSELRKKRVSITYIDHHDMDPEIIKKLQKVKVKIIHDVN
                     ECTTVQVHDAFKSKLTDHSAFVAACAAITDYMEDRPKGAKLLQIYDRQFALISATVLT
                     YNIVGHQKDPDYLLYLVEILSESKYPHEIPNSFEFAQIQVEKLASMIAKVKKSLKTMK
                     NLGYMEILDAGASGAVNFVLGLSGKEVGVAYKERVDHGIYAVSIRGSKSCKVHLGRMV
                     NSLAQEFGGSGGGHDKACGAVIPKDKITQFIKEFNARLNQK"
     gene            complement(26793..27254)
                     /locus_tag="VGA92_07200"
     CDS             complement(26793..27254)
                     /locus_tag="VGA92_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9846235.1"
                     /translation="MIEEKLRSLNIDLPIPPKPAGSYIPVVVTGNLAFVSGQIPMQDG
                     KVVFTGKVPTEKSIEDAQKAAKLCAINILAQLKANLGSLDKVVKIVRISGFVNSAPNF
                     AEQPKIINAASDLFFDIFGESGKHSRIAVGVSSLPLNSTVEIDAVVEIISG"
     gene            complement(27292..>27568)
                     /locus_tag="VGA92_07205"
     CDS             complement(27292..>27568)
                     /locus_tag="VGA92_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HEX9846236.1"
                     /translation="KCLEKNGVSIKDKTLEEILDLVNSTLEITCSGKLQYDQKTNNII
                     LESKVNSGHSLQWAMLIEGYLEQKGQNPKLIYHSDSHKGETVHLKIN"
BASE COUNT         7590 a         5567 c         5596 g         8815 t
ORIGIN      
        1 caggatatgc tgtaactgat gatggcaaaa aactcacaaa gtatagatct gtttttgata
       61 agatcaaaat cgatatagta aaaaacaaag ttaccataac acttgagctg ccaaaaaagg
      121 atttggatgc aagcacagta cttgccgttt gtcaatctta gaatcaaata atattcccat
      181 aattggaaaa taggagcctc tattttttcc aaaaataaat tacatttcaa ctatgtgctt
      241 tgataatagc taaatactat gttcttacca tgttagatta ttaattcatg atatgctggg
      301 aggttaaatg ggaattttct acaattaaat ccggatattg gcagtataga gaactttatt
      361 aaatattaaa aatcactatt ttcaaatgtg gaaatacgtg gacattggcg aaggagagaa
      421 aatctttcat aaagtagacg actgtatact atcaaaaaag acgaaatacc aattactgga
      481 aatcattaaa acaaaagact ttgggaatgt cttgttttta gatggcgatc cacaatcctc
      541 agaggtagaa gagtatattt tcaatgagtg tctggtccac ccagctatga taacgcagtc
      601 caaaccaaaa actgtcttta ttggtggtgg cggtaccggc ttaacattac gtgaagtcct
      661 acgtcatagt gccgttgaaa gagtagacat agtggacata gacaaagaag tagtggatat
      721 ttgtaaaaag ttctacaatt atgcggcaga atccttctca gatcctcgca caaaattata
      781 ccatgaagac gctagacagt ttctttcaaa caccgattca aaatatgact gtatattctt
      841 ggatacgaca atgccccacc acgatgacat tgccgcccca ttgtacagaa aagagttttt
      901 tgaattagcc agtcaaaaat taaagcctga tggagctttt atcacggcgg caaactctgc
      961 agatttcaga tattccacaa gatttgcctc tatagtgaaa acattgcaac aagtctttgc
     1021 ttttgtcaaa ccatacactg catacacgcc gctttttggt caagagtggg catttgcatt
     1081 tgcatcaaat accattgatc caaccaaact aaaaccacat gaggttaatg agaagcttgt
     1141 ggaaacagga tgtaacggat tgaattttta cgatgggcta actcatcaac gtatattttc
     1201 aattgacaaa aaaatgagaa aagttttgaa agaagaaggc acaattttga cagattcaaa
     1261 tcaatctcaa gatgaaatat ttgtcccaga aatatcagaa tcacctgatt ttgaaaattt
     1321 tgtaaacatg attgatttac atcagtactc aagcgtaatt aaactcgcaa gtacgcctca
     1381 caagactttt tgatgtttgt atagcgcata aataataacc atccatcctg cctgccaaaa
     1441 cacattcact acatgggtgt ctgtgtattg accaaatatt tcaagataat aataccagat
     1501 atcggcaaat atgtgtagga ataatccgac ggcaagaagt gaccaaacta ccgcaagtgc
     1561 actttgtttg aacatttggg tgcccaaaag agcaaaagaa gctgaaacag aagctattga
     1621 gacaaagatc atcgtgtaat aataatcaaa accgatatca tgccattctt caattgcaat
     1681 atatgaataa acggtgagaa taaaaactgg aattaaaacc atccaaacta ctgttctttt
     1741 ggttattttc acctcaaagc ctttcatgtt tgtaatcatg tggtatagcg cgaacgggta
     1801 aaatgcaaaa tagaaaatat ctgcaaaaga cggatatggt tctataaaca agatattctc
     1861 gtaaatttgc cacaaaactt cggcaataaa ccacatccca aatgctatgc ctaatgcaaa
     1921 gtatgccttt ccaaagacct tgctaggcca atataatttt gaaataataa aagcgcttac
     1981 tatgaccgca gattgaagtg ataactcaat tatgtcaatg gcgttgaatt cttctgttat
     2041 gtccttaatg gaaatgttga tgacgtgata cactaaacct agacaaaata taatcacaag
     2101 tatcttataa tcaattttgc ttccaatatg ctctggtgta ggatttgatt ttgacaaaac
     2161 taattatcga tttccctaac gtcatttaaa aatgtgctaa ctaatcaaca aatattctaa
     2221 cagaaattac ttctgagcct gacttagatc tatatgataa ttatctgccc tttatatgca
     2281 atgaaatctt aaatctgaga aacgtcattt tcaaaagtgg aaatggtggt atgggaatat
     2341 gataaatttg ttatatcttg taaatcttag ttctgttttt tctagatttc cgagaatcaa
     2401 agttgctact acgttaggtt ttacagttgc tattctttct gctgctttat ctgcattacc
     2461 agatgtaatt acaaaaccaa tagttgatcc tggaacagat ggaatccttc caactgagcc
     2521 acttttggta gtcttaataa tgtacttgat gactggaata ttttttaccc cgcttgcaaa
     2581 attacaaaaa ccacgagtta aaattaaaag atcatcttgg cccatcctca taatttacgg
     2641 tgtagcttct gcttcatcca ctcttgtttt ttcttttgga ttgcaagaaa cgagtgcaac
     2701 aaatgcatcc attctagcta acagtgaaac agtttttaca atattgattg gaatgttgat
     2761 ctgcaaagaa tgcttggcaa aaaaagaact cttgccattg acgcttatag ttgtgggtgc
     2821 gatttttctt ccaattggtt cagatgttta taatcagagt tttgcatttt cagaatttgt
     2881 ttttggggat attttgattt taatctctgg attcatttat tgtctgtgca cttttattgc
     2941 aaaacgtgct agtatgataa aaaccactag tgtagttcaa ataatgtcat tttcaggcgc
     3001 agcttttatc tttattctga tgttaatttt ccagaccgac tttacaattg atcactccaa
     3061 cttctcactt ctctcttttg ttggcattgc gggcataggc ggcagtgtct tgttttttgt
     3121 aatggcagtt aggttgattg gtgcggtccg aaccattcta attttttcat caactacaat
     3181 ctttggagtt ttttattctg gagtatattt actagagccg attagtctgt ttcacatttc
     3241 atctgttgcc ctagtggtgt taggactgca tcagctcaga tataggttag cggcctgata
     3301 tcctgatcca tttatgctta aaaattcgta atttaatgcc acgcacaaat gatctattcc
     3361 caaatttgga aacatgctag tcacattacc aagttctggg cattactaga aatggtaata
     3421 tagtaagaat gtaaatctat ttccatggta ggtatcgacc gacttttgct aaagtcatta
     3481 gacactacaa ttagagaaaa cttgggtgat agaactatta aaaaaattga aaatagatta
     3541 tctgaaaaat acggcatttc actaacccaa tcaatagagg agtttcaaaa actagatatc
     3601 gtactgcgtg aattttttgg accaagcaca gacggactag agaaaaaatt ccttaggagt
     3661 gtgtgtgaaa tcaaattaat ggatggagaa aactgggttc taattgaaaa tcccgaacta
     3721 actaggacta ttttagaatc atttggtgat gatgataaaa agaaaattct tagtgcacta
     3781 aatgggaact cgttgattgt ttcacaaatc cttgaaaaat gcaacattcc ccaaacctcg
     3841 gggtaccgaa aggtaaactc gttaatcaag gaaggcctgc ttgttccttc aggatatatt
     3901 gcagcaccag acggcaaaaa aatcacaaaa tatagatcga tttttgataa catcaaaatc
     3961 gacattctca aagacaagat cgtaataaca ctccacttgc caaaagaaga tttcagctca
     4021 agctctattc tctccgtttg cactcaacaa tagattagga tcaagatatt aggatccttc
     4081 cattaccgta gttggaaatg gcatgggatc cgttaaggac tatttccaag aataaaaatt
     4141 cctttcaact tggatacgtt ctaatcctaa tttcagcagt attggcatca ctagtgcatg
     4201 ttttggcaaa gccgctagtt gacaaccagg tgggatttga gatccaccct gtgacgctgg
     4261 cggcatgcat ttacatcata aacggcgcct tctttacacc gtttacaaga aaatcaaaac
     4321 caatcgcttc acttggaagg cgaaatcttg tgttactggc ggtgattggc atctcagaag
     4381 taattgcgct gattacttat ttttttggcc taaaagactc aactgcagct aatgcgtcaa
     4441 ttttcagcaa cggcgagatc gtcttctcat tactaatcgt gatggtgatc tttaaggaaa
     4501 atctgcgaaa aaaagagatt ggcccatttg ttatgatgtt gcttggaatg atggtcttgc
     4561 ctgtagcata cgacttttat tctcatggca tggtcatatc ggaaatagtt attggagata
     4621 ttctgattat agcatcaggg acattttatg ctctagatgt gatgctttgc agatacctaa
     4681 gcgacaaaat tgactcaaag cgaatcacac aaatcacatc tcttgtttca ggaacaatcg
     4741 cagtagccgc gatgatagtg tttcacattc cgtttaatat ggatcttctt agcttgggtc
     4801 caatcgcatt gtttgcaatc ggcggaaccg gtcttgcaac aatgttgttc ctagtctcac
     4861 tgcgcctaat aggcggggtg aggacgattt tgctgttttc tacaaactct gttttaggtg
     4921 tagtgtttgc agctctgata ctgagtgaga cgataactgt tatgaatatg atatcggtgg
     4981 ccttgacctt tggcggcatc tgtcttctaa gaaacagact aggcggcagc gagcgtactg
     5041 agaatacaca aaaacaaaaa tcagacctag tccggcattc atcgtaagca agcgtattga
     5101 caactgttaa gtgaatttaa ttacatttga atagatttta ggtattttct ttataaaatg
     5161 cctatatttt aaacaaattt ttgtttttct tgtgatctat attatataat ttgtgtgtac
     5221 aataatacta attcgaaaaa tgtaagagaa aaacagagtg tccaattgcg tcttttgacc
     5281 ggtggcaagc acacgcttgc acaaactctt gcaaaggcac aatggtgtcg cttcggattc
     5341 tggtcgggaa gcgcgttggg cactcaaccc ctcctatcat ttttgtgaga atcatttttg
     5401 ttatttgttt tgtcaacatc tagtcaaacc cgaaacttaa atcggacgca aatgttcatg
     5461 cgttgtcttg acccgtgttc gaatgacaaa aaacggcatt gtttgtttta caaatggtag
     5521 aacagtaaac ctagatccga ggtttgcaac gccacaaagt gtaaactttg tctcacacgc
     5581 acacttggac catcttccaa acaaaaaaga gggcctgatt ttggcaacac gtgagacccg
     5641 cgagattgca acacttcgag gccatgatct cttaaaccac gtagaatccc tcgatgattt
     5701 taatttgcat gactctggac acatacttgg ggcaagagga ctacattttg atgatgtctt
     5761 ttacaccgga gatgtttgta ctagggatcg cggatttctt tgcggggcca gtatcccaaa
     5821 atgcaaaata ctcataaccg aatctacatt tggcagacca gaatttgtct ttccaaaggt
     5881 ggacgaagta gtaaaaagag tcaacgagat aattgccgaa ttgtaccaca aaggaaagcc
     5941 ggttttatta cttgggtatc agctcggaaa ggcacagaca ctctctcatc tttttggtca
     6001 ctgggagcca ttgtactatc atgattcagt caaggaaatg aatgacttgc acagacggct
     6061 tggaatccaa ctcaaggcag gacttggcca cacagaggct gaatcaagcg gactacttga
     6121 caaaaagcct tgggtcatgg tatgcccact aatgtcagaa aacaacccat tcatcaagca
     6181 aatgaagtca aagtatgggg cagtaacgat tggcttttca gggtgggcaa catcccctca
     6241 tatgtcatat ggcaggaaaa acgactattc tgtacctctg agcgatcact gcgattacaa
     6301 cgaattagtt gagctggtca aaaaaactgg tgcagaaaaa atctacacgg ttcatggctt
     6361 tgtagaagaa tttgcaaact cgctgaccaa aatgggcttt gacgcacagc cactacggga
     6421 aaactcacta gacgacttta cttgatctca ttacacaaat ctagtacaga attgtaaaat
     6481 aacaaggtag aagcttcact agttttaatt tctgcaaaag ttgaaaccat tctatgtcgc
     6541 aaacagtaca gcaagtagca ggaaaagagc tttgggttct tgttagtcag ctaaaggaga
     6601 atgcagattc ggctgagctt gaaaaaatcg taccacaggt acttgagcta attgatagtt
     6661 ggaccaaaaa tggcaagttc atatggtctg gcccatttgg aaacgagact ggcggtatgg
     6721 caatctttga gggaacgcaa aatgaggcaa accagtttgc tgaaaactat ggaaaaatct
     6781 gctcaaatgt cctaaactac tacacctaca aatgggatat tttgtggggc tcaaaataac
     6841 tatcttgaaa atcaaaacta actttaaatg agatttacaa gccgttccgt atgatgtcta
     6901 ctgcttcgct aaaaaaagat cacgatctta tagaaaagat agtgagggca atggaggcaa
     6961 cgctgcaact gctaaaggca ggaaaaacca ttccggaatc aattctcata ccagtaattg
     7021 atttttcaaa aaactttaca aacgtatgtc atcacggaaa agaagaagag tctctttttc
     7081 ccgcgcttga aaaacatggg tttcctcaga acacgggccc tattgccgta atgctattag
     7141 aacatgaaat gacgcgccag attgcagcca gaatggaaga atctacaaaa gaatacctag
     7201 agtctggtcg ctatgaaaaa cttgttacag acattagtga ctatattgga catgtaagag
     7261 aacacctctg gaaggaaaac aacagacttt ttgtcatggc agaaatgagg ctgcaagggc
     7321 aatcagaaca gatcaacaaa aatttaggcg atgtagaact acaaaagctt gcaagccttg
     7381 ggaggaacag ggccgattat gaaaaactgg tttcggatct ggaacaaaac gtctccaaaa
     7441 tagcctgatt ttagttaaaa aaataaaaat tatgaatttg tggatttatt ccacaactac
     7501 ttgtcctgtc atccaggagt ggtatgtaca gtagtagtca taagtaccag catcatcaaa
     7561 ggtgtgctca aatgtcttgc ctgccataac aatgctactg tcaaatatgt cgtctggtcc
     7621 actggttact gttccactag ttactgtgtg tactgcagtg tcatcatttg tccatgtgac
     7681 tgttccacca acgctaattg tgactagata tggtatgtaa cactcgttag tttccacaca
     7741 atctggtaat gaagatccac ttgggattga aacttcagct gacattgggg cagcttccat
     7801 aactggttct tccactgaaa caggctctgc tggggttgct gtagcgctaa ctgagtagct
     7861 tacagtaaat ggactagtct tcattgtgtg tactgctaac gcgtttcctg aaaactccca
     7921 tgttcccatt gatgtttttg gatcaacaag agtcaatgca aatttggttt ctccgtctgg
     7981 agtccaaatt gctggtgcag tttctccaga tgcaagtggg cctctcaatg atactaattg
     8041 aattggctca gatgctgagt aggctagaat acctgaatag gtatcgtcgc ttggggctag
     8101 aattactgca agttgatgtg attcatgacc aatacctgga tcctgaactg actggattgt
     8161 acctgtcttg acagtgtcgc ttgctgacat ctcctcataa tcgactttgt agtctactat
     8221 gaatggacta gtcttcattg tgtgtactgc aagtgcattt cctgcaaatt tccattctcc
     8281 acgagaattt tttggatcaa caagagtcaa tgcaaatttg gtttctccgt ctggagtcca
     8341 aatcggttgg cccatgtctt cacctggtgc caaaggcccg gtgagtgcga ctagctgaat
     8401 tggctcagat gcgtcataca tcaaagttcc agaataaact ttgtcgcttg gagctagaat
     8461 tactgcaagt tgatgtgatt catgaccaat acctggatcc tgaatggacg ttaatctgtc
     8521 ataccctgca actggtgttt ttgtaggttg tgttggttga ggcgtagaaa gaggttctgg
     8581 aaccggtact ggtggtgggg ttgtagctac tggagctggt gattggctca ttgccgaaag
     8641 tgacatggca attgcaatca gtgcaacagt aaatcctatt gaaaaagcaa ttccgaattt
     8701 gtctgcggtt gtcattggtg cttctgacat ttggacggta aataaagcta attatagtat
     8761 gcaagaattt tgtaactaat ttttctaaaa tctggtctta atcaggtata aaatcataaa
     8821 ttcgtttttt atgatactgt gattgtatct taggcgcagt tatttttctg gtttttttga
     8881 caaactcaca caaatcatca aagatttctc caaagtattt ttttgggttt ttggtcagtt
     8941 ctttgagaag atatggcgtc tttctatgct ttttgtattc acgtactaca taaacaaaac
     9001 ttagggcctt tccaccactg gttttcttga ggatgtggcc atgcgtggcc tggtttctta
     9061 gaatttgcaa tctccacaag ctgctctgtt ttgcattcca accagatttt gtggttttgg
     9121 catgagtagg agttgtaaaa taatctgcaa tgatttgttt tgtttttttc tggtcgccag
     9181 agtctgagag cttttttctt acaccatgaa tgtaaaagac attttcctta tcaaacagct
     9241 tgaacttttc atttatttcg cgttttacta tgtcgactga actggagcaa aaaaacaaga
     9301 acgtctctac atttacttca cagagaaaat gctctagatc tttatttggt attttgtttg
     9361 gaattttatg gagaaagtgc tttgcaactg tgagcttaaa atcaacagga tcaaaaagaa
     9421 tcttttttgc aatcttgtcc gaatcactgc ctgtcatctt ctattacaaa tttgtatttg
     9481 gataaaaatt attcaattcc taaaaagtaa aggcatcttt tagcattagg tgcaagtctt
     9541 cttttaggtg gtcttcattt agactgagct ttagattgga aagcaatgtt tcgattatgg
     9601 tgcactggtt ctttgaaaac tggtagtaac actctaggac atcttcatgc gattttgcct
     9661 ttttgtacat ttcccaaaaa ttatctgaca ttatctggta attgtacaaa aaaattcgaa
     9721 ttggttcttg gacacttgca gacgaattgt atcttagcag gtttatcagc aactcttcta
     9781 gtcttgcatc taggtttgct tcaaaaatag ccttgtaaat gcttgatacg gccagatcta
     9841 atcatacaaa tcaaaataag ataaaactat ccaccagtaa atctaacaga tgttcattac
     9901 attgtgcaat atttgaatga atttatgcaa caaaactagc cctgtccttc gactacagtc
     9961 agagtcatcg tgcttattat gtacttgatc tttcgaattt ttgctgtgat gattttttcc
    10021 agctcccgtt gtgtatctgc caccactttg cagactatct cataataacc aaatacgtca
    10081 tctgcacctt ttacttccgg gatgtatttt agattcctaa ttacatagta tgtctctgta
    10141 ggctcgcaat gtatgatgac ataggcgctt ggcattattc ctgttcctcg tttaccatca
    10201 ttgttatggc cgactcgatg ttttcaatct ttctgagttt ttcaagtacg atcttgcgta
    10261 aaatatcgtc atttgtagct gacatcttga ctatgatgtc gtatattcca aagacgccct
    10321 gaacctcata tgactgcaga cttgaattct tgaggacgga ttttgcctta tcaattatct
    10381 ctccttcatg gtttagcttt gcgtttatca aaaaatacgc aatggacatg gtttattcct
    10441 gcccttcaat tcccatcaaa gtcaatgttg agcgaattct atcgatttta cgaatcttcc
    10501 aagttatgat ctcacgtagt ttctcagcag aagacgtctc tatttttgtg agaacgtcat
    10561 aggccccaaa gacacctctg acctccttta tgccctcaat tggttttatc tggtcgatta
    10621 ctgactgctc cgatccaagc tcgcagttga tcaaaacgta tgccgttgcc atcttactca
    10681 ctcctaccag tattgaaata tttttgatgc aacagcagca tcttcgcatt agccgtatat
    10741 atagtgaatc ataaaaaaac taatcaaatg tttagttttc ttatttactc cttcacgttt
    10801 cttaattaaa ttttgctttg ggcgtacgca gtattctgtt gtatgcaacg ccaaagcgat
    10861 gtgcttcgtc acgagcaaat tggagtatct gcaacgattt tcgatctttt gaaataatga
    10921 ccggctcttt tttgcccggc acaaaaatct cctcgttttc ttttgcaagg gatgcgcatg
    10981 gcacacttaa tgccaagctt tgcagcgcag aaagagcaga gcgcagctgg ccctttccac
    11041 cgtctattag gattaggtct ggcatctgcg aattttcatt atccaaccta aagtacctgc
    11101 gttttattat ttcgtttatc attgcaaaat cgtcttggcc tacaactgtc ctgatcctaa
    11161 atcttctata tcctgacttg tccggcctac cattgacaaa tcttgacatt gcaccaactg
    11221 caaaagacgc accatggttt gatatgtcaa aacactcgat cacttttggt atctggggga
    11281 tcttaagaat ctcttttagc tcaactaggc cgggctcagc acccttggac tctatgagtt
    11341 cgatatttct catgatgagg tctagcattt cttttctttt tcctccgttt ggaacagtta
    11401 ttctgactga aaatcccacc gtctttgaaa ataatccttc aaggagtttg gcattgtctg
    11461 gcatttggct tactatgatg cgtggtggaa ttcgattcgt agtataatac tggaataaga
    11521 aatttgaaaa cgaattgtct gcaaccaaat caaaagaaaa cttgtttgaa tcgcgaatca
    11581 cgccatttat tttcttaaag gtcataatct gggctgtctg atcaattatt ttgaggccaa
    11641 aatactcttc atctgagtca gaaacagtct ccattttctg tcgcgtctta aggctgccta
    11701 gtcgttgtag cgtgtcacgg atttcgcggg cacgctcaaa ctgaaggtgt tctgatgcct
    11761 ccctcatctc cgattcgagt tttttgataa atgcccttag gtctcttccc cttagaatgg
    11821 attctaggtc ggagacgtgc cactcgtatc ttttctgggc ttctttgaac tcgcatggcg
    11881 cctcacagtt gcctatgtga tattctagac atgccttctt tgggagcctt ttgcagattc
    11941 gaatcttaaa ggcctttcgc agagagccaa ttgtcaaaag cttggagctg ccagaggtga
    12001 acggcccgta tatctttccg ccacccaaaa atttaccagt tcgtgttctg cgtgcaacaa
    12061 caagccttgg gtattggtct tctgttagtc tgaggtatgt gtaacgttgc tgatccttga
    12121 gctctatgtt aaagacgggc ctgtattgct ttatcatgtt tgattcaagc aaaaatgcct
    12181 ccgactcgtt gtcagttagg acaaactcga tgtttttgat tttttcgaca agcttctggg
    12241 tcttgtagtt ttgatttttt aggaaatatg atcggacgcg gtttttgaga ttctttgcct
    12301 ttccaatata gatgatcttc cccccgtcat ctttcatcaa gtagattcca gggtgcgatg
    12361 ggatggttat ttttttaatg tttagtgtca atttaccaag atctttttga ggtattgtcc
    12421 tgtgtagctc ttcggatttg ccgcaacctg ttctggagtt cctgtggcca ctacctgccc
    12481 gcctttgtcc ccgccttctg gaccaagatc aattatccag tcagaatttt tgattacatc
    12541 catgttgtgt tcaattacta cgacggtatt tccaagattg acaagtctgt ttagaacatc
    12601 aagtagtttt tggacatctg caaagtgcag accagtagtt ggctcatcta ggatgtacaa
    12661 tgttttacca gtgtcgcgct ttgatagctc tgatgcgagc tttactcttt gggcttcgcc
    12721 tccagacagg gtagtagatg actgaccaag cttgatgtag cctaggccta cgtcgtatat
    12781 ggtctgcaaa attcgcctaa tttttggaat gttttcaaaa aactcgagtg cctcttctac
    12841 tgtcatatcc aagatgtctg caacgttctt gtctttgtag tgaatagaaa gagtctcaga
    12901 gttgtagcgt tttcctttac attcatcaca tctcacataa acatctgata ggaactgcat
    12961 ctctattttt ttgacgccat ctccttcaca tgcaaagcac ctgccatctg ctacattgaa
    13021 tgaaaattgg ccgggtgtat atccgcgctc ttttgcagat tcggtacttg caaacaattc
    13081 ccgaattgga gtaaatactc cgatataggt agcaggattt gagcgaggtg ttcttccaat
    13141 cggggattga tcaattgcta ttaccttatc gatgttttcc atgccgtcaa ctgacttgtg
    13201 ttttccaggc ctttcgtttg ttttgtaaaa atgagtggag agtgtcttta gcagaatttc
    13261 attgactagt gttgacttgc cagagccaga gacccccgtc accgtgacta gtagcccgag
    13321 tggaacctct actgtgatgt ttttaagatt gttttctgcg gcgccccgga tggttatcct
    13381 gcccttctgg ttacgggtct tgttttgtaa cttgatcaaa atcttgtctt ttagataatc
    13441 gcctgtaact gacttgtggt tgtttaggat ttgttctact gttccctcaa agactacctt
    13501 tcctccatgg atgcctgccc ccggaccaag gtctacaatc cagtcggagt ttttgattac
    13561 ttcttcgtca tgctcaacta caattatcgt gttgccaaga ttgcgcagct tgacaagcgt
    13621 cttgatcaac ctggcattgt cccgctggtg aagcccgatt gtgggctcgt caagaacgta
    13681 cagaacgcct gtgagatttg agccaatttg cgttgcaagc ctgattcgct gcgattctcc
    13741 gcctgacaga gttgagctac gtctgtttag tgtaaggtag tttaagccta cgttttttag
    13801 aaactctaaa cgctctgtta tttcctttag tacgtctctt gcaatgtatc gttcagtttc
    13861 agttagagtg atgttttgaa aaaagtcata acattcgtca actgacagat cacagacttc
    13921 acttattccc attccgttta tcttgaccgc tagggcctca ggctttagct tttttccatt
    13981 gcatacattg caagggctgt ctcgcataaa ctgcttgagc cagtcccttt ttgattcaga
    14041 gtctgtatcc ataaagacgc ggtgcaaatt atttagaacc ccctcaaagg aatttgtata
    14101 ttgccaggac gagtcagttg tctgcgattc atactggtag tgaatcatct ggtcagtccc
    14161 atgcaaaatc actttgagct gctcaggcct catcttgtta attggtgtca tcaaatcaaa
    14221 gccaaacttt ttgcccactg cccgaagctc ttgctttcta aacgatgaga acctgccgct
    14281 ccatggaact atggcgccat caagaattga ttttgtcttg tctggaatca ccaagtcagg
    14341 atcaaactcc attttgattc cgagtccatg gcatgccttg caggatccaa acggcgagtt
    14401 aaatgaaaac gttcttggct ctatttcgcc tattgttatt ccgcagtgtg gacatgcgtt
    14461 gttttgggaa aaaaccttct cgtctttgtc agctacgatt aggactgaac ctttggaagc
    14521 cttcagagca gtctggattg cctcaaagag tctgcttttt tctgcttttg atgccttgag
    14581 cctgtcaaca acaatttcaa tgtgatgcca tttctgcctg tcaagccttg gtatttcgtc
    14641 ttctaatagt atgatttggc cgtctacgcg tgcccgggaa tacccgtctt tttttatctg
    14701 ctcgaagagc ttctcatagg ttcccttttt tctttggatt agcggcgcta aaactagggt
    14761 ctgcctatca tcaaaatcct tgaagatgga ctcgcaaatc gactctattg actgatttga
    14821 tattttgcgt ccgcatttta tgcagtgtgg gattccaatt cttgcataaa gcaatctaag
    14881 atagtcatag atctcagtag tggtaccaac agtagagcgc gggttctttg atgtcgtttt
    14941 ttgctgaatt gatatggcag gtgatagccc ctcaatagag tctacatctg gcttgtccat
    15001 catctccaaa aactgtcttg cataagctga gagggactcg acataccgtc tttggccttc
    15061 tgcgtatatg gtatcgaatg ccagagttga tttgccagat cctgagaggc cagatatcac
    15121 tacgagcttg ttttttggaa tatccaaatt gaggtttttg agattgtggt gtcttgcacc
    15181 tcggatcttc agcttatcgt gcatttttct tttccaactc gatttgaatt cgccttagcc
    15241 tgtcgcggca ttctattgcc ttctcaaagt ccaagtcttc tgcatatttt ttcatttgcg
    15301 cctccacctc tattgattcg cgtccaagat cgtgcaatgt cttgttttta atatcatcca
    15361 gtgcagctac ctgctctgga attgatttga taattgttct tggggtgatg ttgtggagct
    15421 tgttgtactc tatctgtttt tttcgtcttc tgccagtctc gtcaattgcg gctcttattg
    15481 actttgttat ggtgtctgcg taaagtatga ctgtgccatc tgcgtttctt gcagcgcggc
    15541 caaaggtctg aatcaggcta gtatagtttc ttaggaatcc ttccttgtct gcatcaagaa
    15601 ttgctacgag tgcaacttcg gggatatcta ggccctctct gagcaggttt atgcctacta
    15661 ggacatcaaa gtcgccaagc ctaagctgcc tgattagttc tgttcgttgt agcccttcaa
    15721 tttcagagtg caaatatctg actctgatct ctttttttga aagataatct gccaaatcct
    15781 ccgccattct ctttgttagt gtggtgacta ggacgcgctg gttttttttg gtcctttttt
    15841 ggatttcaga aatcagatca tccatttggc cctttgttgg tcgcacctca actgttgggt
    15901 caatgaggcc agttgggcgg acgagctgct cggcaatttg atacgatatt tctttttcat
    15961 atgttgaagg ggttgcagaa acaaagatgc agttctttat gtacgattca aactcggcaa
    16021 attttaacgg cctgttatca aacgcacttg ggagcctgaa cccataatcg actagtgact
    16081 ttttacgtgt gtagtctcct ccatgcatgc catgaagctg cgggattgta gcgtgggact
    16141 cgtcaatgac tagcaaaaaa tcatcgccaa aaaaatccaa aagacaaaat gccttctcac
    16201 caggccccct tccatcaaaa tgtcttgaat aattttcaat tccagaacaa tacccgagct
    16261 cttctatcat ttccagatca aacttggttc tcatctcaag tctttgcctt tcaagctcgt
    16321 taagctgcgt tagcctctgt tttagctcgt ctcgaattga cttgactgcc ttttgtcgaa
    16381 cgtcgtctgc aatcaggtag tgttttgctg gaaatatctt aattgaattt agcttgcgct
    16441 tttcttttag cgaaaccctg tcaaggagca ctattttttc tatttgatct ccaaagagtg
    16501 agatcctgat gatgtcctca gaatatgccg gaactatgtc aattgtgtcg ccttttacgc
    16561 ggaaattgcc tgggacaggc tccaagtcgt tgcgctcgta ccttgcgttg atgaatttct
    16621 ttatgagttc tgttctgctg taggtggcat tggcagtaac tgtggttgcc atctcctccc
    16681 actcctttgg ggagccaaga gaatagatgc atgaaactgt tgccacaatt attgtgggtt
    16741 cgccagaaag gagcattgcg gttgcctcaa ggcgcatctt ttctattttt tcgttgatct
    16801 cagtgtcttt ttctatgtat gtgtcagttt gcgcaatgta gctttctggc tggtaataat
    16861 cataatagct gacaaaataa ccgacattgt tttttgggaa aaactgtttt agctcagagt
    16921 acagctgcgc agccaaagtc ttgttgtgtg aaattacaag tgtgtttttg ccagtttgtg
    16981 caaccacatt tgcgatggta aatgttttcc cgctgccagt gacgccaagc agtgtctgaa
    17041 ccttgttttt tttgacgccg tcgactagct ttgtgactgc ctgcggctgg tcccctgttg
    17101 gctcaaattc tgatacgatc tgaaatgtct gcacttgttg caacagatcc gatcacactc
    17161 atcgaaattt aaagcaatgg agcttttgtg aatgcaggct tagctaacac tactcaccaa
    17221 aggggctcat caactagccg taattctttt ccggaaacat caaagcccat aattttgagt
    17281 gtttcttttg ctgttgcgga atttcgctca aagatttttt ttgcaagttc tgttcttgag
    17341 gccgtgttca tgtgctggcc aattttgtat ttgccgtgca ttgtctttgg cactactttg
    17401 attattgcaa cctcatctac tacttccatg tctggagtta ttttctcata ttggccttct
    17461 ggctggtatt tttccataag gccgtttagt gccagtgtct tttcttgtct atcttcaatt
    17521 attgtgccgg ttcccttgat taccacacta atgtacaggg tgtctgcctg tgatgcgtca
    17581 gttggatgtg taaagtacga tggcaaaaac tctagttctc tgtccacctc aaagcctact
    17641 ttactgttgg cttggatgtt gtcaagcttt tcgcctcttg tatgagaatg catgtaaacg
    17701 gcatcgttta gaaacacaaa gttcatcgga attacctgcg ggtatccgtc cttgtcaatt
    17761 gagcagattc tgccaacatg ttcctcattt agaaactgga ttatcttctg ccttgacttt
    17821 atttccagcc tgcctaagag ctgcatgata aaaattcgtt ttttatctaa ataaatgcaa
    17881 tcattttaca aatctcgctt ggggaaaagg ttccccaagc atgggtacag gtgtgttggt
    17941 acggcagagt tgtaaaaact ctatcagaat aaaataacta tttttgaaat aaaatgcttg
    18001 cgtagaaatg atatgaaact atgtttctga ccactcgtat atggaaaagg caagaccctc
    18061 gtctttttca gcatccacga tctttttctc aaacacatat cggtaaatga tgctgtcgtc
    18121 atttatgatg tcaaggccag cttctttact tgtgccaatc tcctttgaca gtatggtaag
    18181 cagaacattt gagaggctgt ccaggtcttt ttttgcatga gcagaatctt tctgcagaaa
    18241 aaaagtgaca tttattgaga gcggttttcc agcacaacgc tttctgacat tgtttatgtt
    18301 ttttccaagc tttgtttcaa tggcatcaag gatctttttt ttatccgcct taacatttct
    18361 tccagcagta gctggaacaa agccctttac ctgtatgttt tgtagcaaaa catcgccttt
    18421 catgcagagt ctgaaatgtt gtctaaattt aagctagtgc tgcaaacaaa atatgatttg
    18481 caagtctgtc tttttgtatt cagaacttga tcggcctttg cctaaaatga accagagtct
    18541 catttatgcc atcaagcaac ctctcgactc caggtgttgc agacttgttt tcggcaattt
    18601 tcttttttag cacattaagg tggtgaatgt tttgcttgag tatcttgccg cgctttgttt
    18661 ttgacatttc tgattccttt atcgttttga ggtttgcaga aatctcacat aatttgatta
    18721 gaattgatcc ccagggtgca gcctgaagct gttctatgta ttgttcttct tgtctttgcc
    18781 ttggcaaggt gaaatctttg gatattgagg caacagatgt cgctatctcg cgcccaaacc
    18841 tggaatatat ctcatcaaag cttgccttgg tttgacacaa aaatgcagtg ctaagaacct
    18901 gctcatcact tatgccaatt gattttagcc taaagactat gcctttgcag aatgcaatgt
    18961 ctgcattttt tgcccttgca taagattcag cttcttgaat tgtaaccata tgagtaggta
    19021 aatgggattg gactaaattt acttgtattt ttctttcaaa tacggcatta ctttagatgc
    19081 aaacttgtca atagatccaa aataattaga gccccagaat cgtattacaa agtgattaac
    19141 accggccttt aggaatcgct caaagactgg aatcacatca tctggagttc caactgccac
    19201 agaagagcgg gcaacggcat ctggaattgt agttgcagct tctctcatct ttacaatcca
    19261 ttcttggttt gacattgaat attctgtgaa atattttttg aaatcaaatc cctcgatttg
    19321 ttttatgttg tgcactcgaa gtacttctgg cttaaacaaa gaaactttga cggcttcttt
    19381 catttttgcc caagattctt ccgcgtcttc tgaaaaatac acatcgatgt ctagcgcata
    19441 ctgaaagttt tcttggtctt cttttgatct accttcttct ttcatcgagt ctttgattgt
    19501 ttttacatga tcctcgaaca gctcaggagt gtatccgatt ggcagccatc cgtctccgta
    19561 ttttccagtc atcttgagtg tacgttgtcc acctgctgcc atgtatattg gcgggtgtgg
    19621 ttttcgaatt ggttttgctt gtagacacgc atctgataac tgataatatt ttccattaaa
    19681 gctgactctg tgctcaggtg atgactcgta caacaacttg atgacctgta gctgctcttc
    19741 ccacttggaa acgggatttt caaatgatat gttgaactct ttgagatttt gtgcctcgcc
    19801 tgcccctatg ccgagtgtgc ctcttccctt tgagaccctg tcaagtgtaa ttgaggcaag
    19861 cgcaatgttt gatggatgtc ttctgatggc atctgtaaca caggttccaa gctcgactcg
    19921 ttgtgtggtt gcagcaatag cagaaagcat tacccaagga tctaagacag tagacttgga
    19981 ccattgcggg acattagagt ggtccatata ccaaatggaa tcgtatccgg ttctatcagc
    20041 taaaagacat gcagtgagaa tttggtcctc agaaaatcca gctcgtgcta cattaagccc
    20101 gttttgaatt ccaaatttta gcatcttttt tttgatctca tcttgcggta tttaagttta
    20161 ggactctatg tgagatatgt tccgtagatt acagagtctt gcaaactaaa aaagaaaaga
    20221 ctagagtctt ccttctacaa tgtcttttag acactgtatg acatcttctt ttgtgacagg
    20281 tcttgggttt gggtctaggt gtcccctgtc tgtcatcaca agatcagctg cttggtcaag
    20341 tggtgccttt agtgcgagcc tatcaaattt gagcttttca attacctctt tgaatctgtc
    20401 ataaaagatt gacttgttga acttgtgtgc aactgtggtg catgatgaaa gcgagtatcc
    20461 atgtggcact ccctcatttg agaacacgta tgacaatgca tgtccaagtg ttgtagagca
    20521 attaccgaat cctattccgg aaagcatcga gccgtacgga taattttctg gcttgttgtt
    20581 gatgattgcg tcatatagaa cttcgaaggc ttgcttgcac attgttcgtg taaactcgtt
    20641 tccaatcttg ctgtcatatc cttcggttgc ttgagcacac gcatcacaaa ctgagttttt
    20701 gacaatttgt tctggggtac cgtccataaa ataggcatca actactgccc tgtctgccaa
    20761 aaatctgtct tcgcgcaaca gcttcttttt tccttcaaat ttgaggacgc agtatgtggt
    20821 catttcggcg ccagttccaa aggtagtcgg aattagaatt ttttcaagct tcatttcaga
    20881 gcctgcatat tttgcgacat caagtgaact tccgccgcca aggccaatta ttgcagacgg
    20941 gttttttgat ttgaaatcag caattacttg ttgtactgtt tcaattgatg gttctggctc
    21001 cactttgtcg tagagcatat agtccttgat tcccatcttg cccagccatt ttgttgatat
    21061 tggcggtggg gcagtggtga ctaccaaggc attttttgga tattctgctt ctgaaagcgc
    21121 atctttaccg aaatggataa tttttggaat tctaatcgtg tgcatgtttg gtagtaaaat
    21181 agtatgccta attatatctt acaggaggat acgacttcgc caataagcct tgatgcaaga
    21241 tgtgatgtta catcatttag atcattgttt ggacagacct ccattacgtc aaaccctatg
    21301 attcctcttt gtacgatttt tttaatcata tatgtaacat cctgactttc aagaccaatt
    21361 gggactggaa ctgagacgcc gggcgcatat gcaggatcaa ggcaatccat atccaatgaa
    21421 acataggtgt ttttctttac ggtatcaagt attactttgg tgatctgttt tacgcccttt
    21481 tcaattatat caaagggggt gattacgcga aggccgtgct tctttatgtt gtcgacctct
    21541 tcttgttcag gagtacgagc gcctactact atgcttgatt ttatgtcaag atagtccaag
    21601 gaatcagtaa ttatggagcc gtagtagttt tttgtatggc tgacaaaatc cggatgtgca
    21661 tcaaaatacg ccaaagatat gggagcgtgc ttttttgcca acgcttttag aattttagcc
    21721 gtattggaat ggtctccacc aatcgagatt gggatttttg acgcaaagga aattctgtca
    21781 aaaacctctg ggatctgctg ttttttgata ttcccataat catagacttg ggtatttgag
    21841 ggtccttttg ttggaagggc aaggcatctg agttttcctt caatgtagat gtcgcgttca
    21901 tttgagattc gccttatggt gtctggggct tttgatgtgc catcagagta tgaatgggag
    21961 cctgattcat ccggtattcc tactataaca atatccgaat catcaaagct tgatgtgttt
    22021 gcccaacaga ttttcttgaa tgcttttggt ttcttagcca atttgaattg taacttgact
    22081 aaacaagtaa tgaaaataac tttttctacg atattaagcc ggcagaatga aaattagtag
    22141 agctgatctt aaatgatctg acaaatacca caggatgttg ataaaaaaca aaaaacagat
    22201 ctacaacgcc aaaaccaaag atgcaataca aattttagaa gcgggccttg atgccgcaaa
    22261 acccgaacgc attcttttca ggtttgttag aaaaggaaaa ctagatgtga acaaaaaatc
    22321 atatctctta tcaaaatatc gcaaaataca tcttgttgct gttggcaagg cagcggattc
    22381 gatggcaaag ttggttcacg acaaaataca tgtagattct ggaattatcg taataccgca
    22441 aaattataat tcgatgcttt ctgcaaaaaa ttttaaaata tttcgggctg gccacccaat
    22501 tccaaacaaa acgagcgtca tcgcagcaaa atcaataatc aacttagtag agggggcaaa
    22561 aaaagaagac ttggtaatct ttcttgtttc tggaggtgca tcggcgttga cttgccttcc
    22621 agatggcatc gctctgggcc aaaaacaaca gcttacgcag atacttttga ggtgtggcgc
    22681 atcaattaat gaggttaatg cgctacgaaa gcacctctcg caaataaagg gaggcaggct
    22741 acttgagcac ctgcattgtg ctgccgtatc ctttgtgatg tcggatgtgg taggcgatga
    22801 tctttcatcg atcgcatcag gccttaccta ttgtgataaa accacatact ctgagtgtct
    22861 tgggatcatc aaaaaatatg gcatagaaaa acaaatttca gcccaggccc taagacagct
    22921 taaactggga gcaaaaggaa aattcgcaga gaccccaaag aggccaaaaa taccaaacat
    22981 agttgtcgca agcaaccaag attgccttga tgcgatgaaa aatactgcaa ctaaacttgg
    23041 atacagaaca aagatttttg cgccgcttac tggcagcaca aactctgcgg caaaaaaaat
    23101 cttgcaaaat ttttctttta ggaaaaaatc atgtctgatt tttggcggtg aaacaacagt
    23161 caaagtcatc ggggacggca ggggtgggcg aagccaagac ctcgtcctac agatcttaaa
    23221 taggatgatg gaaaacactg tcgtcgcagc catgggaact gatgggatag atggaaacac
    23281 aaaagatgct ggtgcaattt tttacatgat tccaagaaaa aataaagtaa gggattatct
    23341 aaaaaacaac gactcgaact cttttttcaa aaaatatggc ggtcttatca cgactgggcc
    23401 gacacacacg aacctgttgg atattggatt gattttaaaa tcgtagcatc tttgatgcaa
    23461 gattttgcgt cggatcaatt ttttcatatt gtaacttgag gtttctttgt gaggcaagtt
    23521 gatttatgaa tccctttcta ttttctgcaa acgcatcttg tgatttgtca tctgatgaca
    23581 aatgtgcaag catctcgtct gagtccagca ttattttgac tgtgtcgccg ttctttatga
    23641 taaatacgcc aatcgaccaa aaaaggccta cgtgaagtgc aatgtacttt gattgcaaat
    23701 tagttacttg gtccaaaaaa atttctgcct gttctctgaa ctgttcattt atgatgtcct
    23761 gtgttttaat tgtccagcaa atcctttttg cattgccatc ggagtgcaga gtatgctcca
    23821 ttttttaaca ccgaaaaacg tgactgtttt gtctatataa tttgaattca gctctgaata
    23881 accaatcata aaacataccc gtgatgcaaa agtcctttgt cttctttaca aggatggttg
    23941 atgagctagt cgagttttcg gactctgatc cagaactcgc agaaggaatt cgttggctcg
    24001 atatgcaggc acaaaaaaag ggaatctcat tttatgagat ggtctttcaa gtcttgtaca
    24061 aacacgacat aaacacgcgt gcacaacaat ggcttaactc tagaaactaa tctgataatt
    24121 cgcttgccct tttgaattca actttgtgtt tgcctggaat gattttaaca ggcggctcaa
    24181 ttctgggctt tttctttttt gccagttttt tgcgttttgg ctttttgccg taatcatccc
    24241 attttatcca gaccatgtaa cgtcttggtt tttgtcggat taattattat cttagatcga
    24301 ttggcttgta ctcgcatccc tgtggaccac agtattttcc aacataggta gcctttggcc
    24361 tgtataacac aggttttgga gcagcagtgg caaacttttc ctcaattacg tgtgcagtcc
    24421 agccagcaac gcgagagatt gcaaaaattg gagtgttcaa gtcagatggg atctttagca
    24481 tgtaatacag tgaagcgctg tacagatcta catttgggta aatgtcgatt cctttctgtt
    24541 ttttcatttc acgaatagta gtttcttcta ctctttctgt tatatcaaac caaggagctc
    24601 cagtcttttt ggcgagcctt tttgaaagct ccttgagaac ctgagccctt gggtcgtacg
    24661 tcctgtatac tgcgtgaccc atccccatta ttttttcacc cctggcaagt ttttctttga
    24721 cccaagtctc tgcgttatcg gcatttttga tttccaagag catcttcatt acttcaaagt
    24781 ttgcgcctcc gtgaagtggt ccagatagtg ctccaattgc cgcactcact gcagagtaca
    24841 tgtgtgcacc agtggatgct acctctcgcg ctgcaaacgt tgatgcatta aacgtgtgat
    24901 ccgcatgcaa aattaaacaa atgtcaaaga ttctctcagt ctcagcatca gggctttcgc
    24961 caactaacat gtgcaaaaag tttgcagaat ggcttagtct tgggtctggg tctacgatgt
    25021 ctttttgggt tctcacgcgc tcccagcttg caatagttgt gggtattttt gcaattagat
    25081 tgatggcact ctgatggttg atctcttctt catttgcacc cttgtcatca tagtatcctg
    25141 ccagggccac aacgaaggcc tgaagcatca ccatggggtc tgcatttttt cgccagttcc
    25201 tcatgtttga ttgcatctgg cgtggcatgt ctcgagcacc aatcaattcc tgcttgaacg
    25261 catcaagctg tgcacgggtg ggaagatcat cgtttagaag caagtaagct gtttcctcaa
    25321 aagtagagtt tttcgtcaaa tccaaaatgt cgtagccgcg gtagattagc ttgccatgct
    25381 ctccgtcaat gtcggagatt tttgtatcgg caacctcgat gtctcgtaag ccaatgtttt
    25441 ttgtatccaa tttggtattt ttgatcaaaa tttcactata aagttttgca caatttagca
    25501 tttggtctaa tctagcaaag cttgtcagtt tagccttgtt tttcattaaa aatcatgagt
    25561 caagctgagc gtgattttat caagcaaata gatgacctaa tacttggcgc atcgcaggaa
    25621 gatctatcca agattgcaga gcttgacaaa aagacccaac ttgacggcaa gacattttat
    25681 gatgtctatt tggcgctctc cgataaagac agaaagcaaa ttcatgattt tgtgtcaatc
    25741 aaaaaacgat aatcttgctt tttgatttaa attgaggtag ccgagcagtg cgggtgtgac
    25801 ggctgcaaaa aaaccaaaag caaagaaaac aaaaataatc tgcctttccc acatggagga
    25861 tgccgacggg gtctcgtctg ctgcgctgat aagacaagta tttggagggg acaccattct
    25921 agttgactat cctgggatga tgcctgcaat cgaggcgctt gcaaaagacg aaaaactcgg
    25981 ggccttgtac atttgcgatc tggggataag caaaaccaat caagacaagt ttgttgaggt
    26041 tttatctgag cttcgaaaaa aacgtgtctc aattacttat atcgatcacc atgacatgga
    26101 tcctgaaatc atcaaaaaac tgcaaaaagt caaagtcaag ataattcatg atgtaaacga
    26161 atgtaccaca gtacaggtcc atgatgcatt caaatcaaag ctcacagatc actctgcatt
    26221 tgttgctgca tgtgcggcaa tcactgacta tatggaggat aggccaaaag gcgcaaagct
    26281 tttgcaaatt tacgataggc agtttgccct aatttctgct actgttctaa catacaatat
    26341 tgtgggccac caaaaggatc cagattatct gttgtaccta gttgagattt tatcagagtc
    26401 aaaataccca catgagatac caaactcttt tgagtttgcg caaatccaag tagaaaaact
    26461 tgcaagcatg attgcaaaag tcaaaaaatc actaaaaaca atgaagaatc ttggctacat
    26521 ggaaattcta gatgctggag caagcggtgc ggtcaacttt gttctgggtc tgtcaggaaa
    26581 agaagttggc gttgcgtaca aggaacgagt tgatcatgga atatacgcag tatccattag
    26641 gggttcaaag tcgtgcaaag ttcatcttgg aaggatggta aactcccttg cgcaagagtt
    26701 tggtggctcc ggagggggac acgacaaggc ctgtggcgca gtaattccaa aagacaagat
    26761 aactcaattt atcaaagagt tcaacgcaag gctcaaccag aaataatttc aactactgca
    26821 tcaatttcta cagttgaatt tagtggaaga cttgatactc caactgcaat tctggaatgt
    26881 ttgccgcttt ctccaaaaat atcaaagaac agatccgatg cagcattgat tatctttggt
    26941 tgttctgcaa agtttggtgc cgaatttaca aatcctgaaa ttcggacaat ttttacaacc
    27001 ttgtcgaggc ttccaagatt tgcttttagc tgggccaaaa tgttaattgc acataacttt
    27061 gcggcctttt gagcatcttc tattgatttt tcagttggca ccttgccagt aaagacaact
    27121 ttgccgtctt gcattgggat ttgtcctgat acaaacgcca gatttcccgt aactactaca
    27181 ggtatgtatg agccggcagg ttttggtgga attggcagat caatgtttag ggaccttagc
    27241 ttttcttcaa tcatgatatg tggctcttgt ttttggtgct tttctgtctt actagttaat
    27301 ctttaggtga actgtttcgc ctttgtgcga gtctgagtgg tagattaact ttggattctg
    27361 gcccttttgc tcaagatagc cttcaattaa cattgcccac tggagcgagt gtccgctgtt
    27421 taccttggat tccaaaatga tgttatttgt tttctggtcg tattgcaatt tgccagaaca
    27481 agtgatttca agcgtagaat taacaaggtc taagatctct tctagtgtct tgtcttttat
    27541 tgacacgcca tttttttcta ggcattta
//
LOCUS       DASWKH010000093        11227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1345771, whole genome shotgun sequence.
ACCESSION   DASWKH010000093 DASWKH010000000
VERSION     DASWKH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345771"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /gene="fen"
                     /locus_tag="VGA92_07210"
     CDS             <1..920
                     /gene="fen"
                     /locus_tag="VGA92_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HEX9846237.1"
                     /translation="SIIRGPDGTLLSDAKGRVTSHLSGLFYRNVNFLSLGIKPVYVFD
                     GKPPSLKFAEIERRKQIKKEATIKYEKALVQGNMEDMRKYAQQTTVMQDGMVDDAKQL
                     LDLFGIPHIDAPSEGEATAAHLTQTGLAYAAASQDYDSILFGAKRLVRNFTNSGRRKI
                     PNRNTYIEIEPELIDSEKMLSDLGVTREQLVDIGILIGTDFNPDGFERIGPKTALKIV
                     KENGRLEDIPQIQDKLSQIDYKQIRKIFLEPKVTPVSEIKFGTVNYSEIVRYLSGERS
                     FSKERLESSLGRLQKSTEKRSHTLEQWFT"
     gene            complement(952..2670)
                     /locus_tag="VGA92_07215"
     CDS             complement(952..2670)
                     /locus_tag="VGA92_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX9846238.1"
                     /translation="MEKQNVSTPSANILDHFPDGFKPRQIQIDLLRQIQDRLSEGFGT
                     LLLSAPTGIGKSLIAGAVAKHFGRSFVVTASKHLQDQYSHDLPFFKPVKGESNFACLK
                     LMESKKIDFEETDFAIKRSLTCDIGPCDMKKTKGGEQIMDKCNFKPAISEIESGKIQE
                     NACFYYLQKYIGLVSPHTIWNYYSFFQMIKFQQKYFEDYLSRAVTIFDEAHRLEDQII
                     GFVGLDIYKGNLEECGINAEHYDLSDINMVIGLIEDLTRSYARQSGELLESRIFQMKP
                     NYELLERLEKKYERFLHAKNEIKSNAENFVINKPERDENGGFVSVSVKPLDISKHLDA
                     FLTSPVRIFMSATIEPASFCENTGIPVSEVAFIETPKSPFLLENRKINFLNVKALNYA
                     TSNEDNMEVIRTIDSLLKIHEDKRGLILTSSKERCYMIYNNLSEVNKKRIRICHSKND
                     DDLTVDDILEAHKADPRGVLLSSSLWEGIDLKDDLSRFQIIEKTPFPNLTEKWVKEKS
                     AKYPLWYRSKTLMKLLQGCGRSVRNDTDWAKTYLLDSNAWRLVSDLKKFVPQAYHDVL
                     YLTNYR"
     gene            3039..4349
                     /locus_tag="VGA92_07220"
     CDS             3039..4349
                     /locus_tag="VGA92_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9846239.1"
                     /translation="MEMPENLKLNTFLKEFRQKCHNKKCDFGTYGFSFGQSPFPVPNI
                     VQKALIKNAGMGTYASVSGILPLREAISEYNKYYFDVDVDPRRILIGNGTKQLIYNIL
                     EMLHGTVILPTPAWLGYLPQIRFLRKNFHMLATRRNNGKIIPEELKHLALRLHDRQKI
                     LFLNNPNNPTGRLYERLELEEIVDICRMYNILVISDEIYAQTTYDFKKFVSMAEIYPE
                     GAFVTNGLSKSHSSGGYRLGYVILPHHSSEILQNFKKIQAAEYTSVSTPIQHAAVAGF
                     QPSKEINKYFETTRNIHHMMSEYVYESLNEIDDVRTTKPEATFYLLADFNAFTDDFHR
                     HKIITSQQFSESIIGHPYHTAIVGGDSLVLERTDFSARIAFVDYNGASTYQNYQNHKP
                     KNQSDRTEFVKKNAPRIVDGLDMIKKYFGDLTKSKMRVKSRASH"
     gene            complement(4365..5663)
                     /locus_tag="VGA92_07225"
     CDS             complement(4365..5663)
                     /locus_tag="VGA92_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9846240.1"
                     /translation="MVEIAISSLIGDSLDKMQQAIPQFSFPEVAELGATTLLLSAAII
                     IFLGVVGEAFFRKTGIPDIAFLMVLGVIIGPVFGVIPQDVIVQIVPYFAAVALIIIMF
                     DGGLGLDIRKVIKTAHFALALSILGFAASVISVAVVTYYGLNWSLIESVLLGTIVGGS
                     SSIIVFGLVKQLNISEETKSMLSLDSAVTDILVTVIAFVLIDVLVAGYLNQDLAIERF
                     AKSLAVGTVLGFGVGIPWMFAMTKLQKAQHAYMLTIGILFVIFFLDKSFGGTGALAPL
                     IFGLILGNKQTLSKYLRFKIPEIPSDNSVHHELTFMVRSFFFVFIGLLASITQLEYVF
                     YGVIGAILIYIARIGVVKGILTKRFNKFEKKVTQAMIPRGLAAAVMATIPLTMGLQKG
                     EVYPQIVFVIILTSVIITTIALGRAKKFNIPVTDESKNNK"
     gene            5777..6133
                     /locus_tag="VGA92_07230"
     CDS             5777..6133
                     /locus_tag="VGA92_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846241.1"
                     /translation="MNLQLAAMAVFAALLVSVTLADISFAQTTPKNDIKSIMENYRKA
                     ILKAQTDFQAAIKKANVDAKDVVLKGLPIDQINADSKSKIQKARVDLKASIAEARVDA
                     KNALLQLKAAIDAKSS"
     gene            complement(6150..6422)
                     /locus_tag="VGA92_07235"
     CDS             complement(6150..6422)
                     /locus_tag="VGA92_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846242.1"
                     /translation="MVAALVLIQNKAFAVASDSIYNIHYKVSANNVNMVPIVIMASIL
                     LVVLMAYYKISKVEKYDTFSLKCKKCGSFTRGLKCVVCQSRKMATK"
     gene            complement(6538..8442)
                     /gene="acs"
                     /locus_tag="VGA92_07240"
     CDS             complement(6538..8442)
                     /gene="acs"
                     /locus_tag="VGA92_07240"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9846243.1"
                     /translation="MNSEIFTIGLGNNDAKTRLEASKDYVSFWEKQAQKLVWSEMWSK
                     VLEWTPPFARWFVGGKINASYNALDVNQKQKLKPAILWEGENQERRTITYGELFDEVQ
                     KLSNALKSVGVKKGDRITIYLPMVPEIIVAILACARIGAIHTVIFSGFSATSIRDRAL
                     DSQSKIVITADGGYRRGGIVKLKETVDVAIENLDFVQNVIVLRRIGNQITLNKKDLMW
                     EDVVKNASNVCDAEPLDSDHPLYILYTSGTTGKPKGVLHDTGGYLTHISSTFRWAFDI
                     KDSDVYYCTADIGWVTGHSYVAYGPLICGATQVMYEGAPDYPSPARIWDIIQRYKVTI
                     FYTTPTALRMFMKFGDTIPNSFDLSSLRLLGTVGEPINPEVWKWYFRTIGKNRCPIID
                     TWWQTETGGIMISPLPGLETMPLKPGSGSFPIPGVDLAVVDENGAEVKHNTKGYLVIR
                     KPWPGMLLTLWGDDEKYKSVYWSKYDGFYYTGDYAIKDKDGYFWLLGRADDVLKVAGH
                     RIGTAELESSLVSHNSISEAAVCGVPDEIKGEVIIAFLVTKEGVAVTEDLRKEVLNRV
                     RTGIGAIATPHQIYFVSNLPKTRSGKIMRRLLKAIASNEKIGDVSTLEDGAAVSEIQD
                     AFDDLKKQIR"
     gene            complement(8503..9432)
                     /locus_tag="VGA92_07245"
     CDS             complement(8503..9432)
                     /locus_tag="VGA92_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdvA-like protein"
                     /protein_id="HEX9846244.1"
                     /translation="MNKDEIEIIGKQVKDMYGTYMGKVIGTITDIDGSIQTVGIDCGL
                     DGLKQVPFEQLVVQGDVVIFIPKWRLDAQRFLREKGLTIRRLKALIDIVSENDEMRED
                     AEIIHEKYKSKLLSLEESEKEISTTLAARLEELNAQLKLVKTLLFDAKVQFKSNEISE
                     SKFDLVKTHTGELIEHIDHEKAEILNIQRRIADIALDGVKTDVNQQVQIQQSAVSYLV
                     TNTASGQQVETPNVPPSTSDATQSTGDANKTTNTDAEQQEPVTANIAPPSAEPNKDAP
                     KWHAAIPQVTPTQQSGGFVNTHSENTGSECLEA"
     gene            9561..9836
                     /locus_tag="VGA92_07250"
     CDS             9561..9836
                     /locus_tag="VGA92_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846245.1"
                     /translation="MIDRDGLNQILSFVSKRWTDLSRDSNNKALQNLPADFWMNSVGG
                     KAGNGHWITMLMYTENENDANALKEVLLRWQTKGKSSTTDLIQINRK"
     gene            9867..>11227
                     /locus_tag="VGA92_07255"
     CDS             9867..>11227
                     /locus_tag="VGA92_07255"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX9846246.1"
                     /translation="MAEEFTGSEKEVLLKHFSNAEDSVFAIITPRQVDRGALMSRYSR
                     TDKSMRRIFLDEFLANESRGEEFYNKVLIEYGDDSVAELGIAQIAIEGISNIAVKKIE
                     DRRIGLSYLEKSSRYVSWDKKVNGHFKFYREPQIMDSKFANAYLDSCNLDFEIYSKNI
                     QPMIDYVREKEPIDKLKFKDTLSGTEVFFSKLKDDRDIKSATMIYNATTKAKALDVMR
                     GLLPASTLTNVGITGNGRSFEYLLTILFSSNLDEEQNLAHKIKQELDTTIKSFVRRSD
                     DKYGKALQNYFKSLQKTSSRLVKKHPVSTKSKGATVKLVDYEPELRALDKIIVAILYE
                     GQTVTYNAILSNVKKMSKSEKTKIITSFAKLRQNRRQRPPRAFEMTHYTFDIVGNFGM
                     FRDLHRHRVLTLERQLLTTDHGYVIPREIAEIGLEKEFKDCMDVSKRVFDSMRRKIPQ
                     QAQ"
BASE COUNT         3378 a         2364 c         2176 g         3309 t
ORIGIN      
        1 tatcaataat cagaggtcct gacggcacat tactctcaga tgccaaaggt agagtaacga
       61 gtcatctgag cgggctgttc tatagaaacg tcaactttct ttctctgggg ataaagccag
      121 tctacgtatt tgatggcaaa ccaccttctt tgaaatttgc agagattgag aggcgaaagc
      181 agatcaaaaa agaagctacg atcaagtacg aaaaggcact ggtccaagga aatatggagg
      241 atatgcgcaa gtatgctcag cagacaactg tgatgcaaga tggcatggta gatgacgcaa
      301 aacaactttt ggacttgttt ggaattccac acattgatgc cccatcagag ggcgaggcta
      361 ctgcagctca tctaactcag accggtttgg catatgcagc tgcaagtcaa gactacgact
      421 cgattctttt tggagcaaaa aggttggtga gaaattttac taacagcggt agaagaaaaa
      481 ttccaaaccg taacacctac atcgaaatag aaccggagtt aattgactct gaaaaaatgc
      541 tctctgatct tggagtgaca agagaacaac tagtagacat tggaatactg attggtacgg
      601 actttaaccc agacggcttt gagcgaattg gccccaaaac agcactcaag atagtaaaag
      661 agaatggcag actcgaagac attccacaaa tacaagacaa gctttcccaa attgattata
      721 agcagatacg aaagatcttt ttggagccca aagttacgcc cgtatcagag ataaaatttg
      781 gcacagtaaa ctattcagag attgtgcgat atttatcagg ggagcgaagc ttttcaaaag
      841 aaagactcga atcgtcgctt ggcagactgc aaaaatccac ggaaaaacga agtcataccc
      901 ttgagcaatg gttcacgtag acaaacacat ttgctacgaa tatgttttgg attagcgata
      961 gtttgttaga tacaaaacat catgatacgc ttgtgggaca aattttttca gatcacttac
     1021 aagtcgccat gcattggagt caagcagata cgtttttgcc caatctgtat catttctcac
     1081 gctacgacca cacccctgaa gaagtttcat caaggttttt gaacgatacc aaagtggata
     1141 cttggcgctt ttttctttga cccatttttc cgtaaggttt ggaaatggag ttttttctat
     1201 gatttgaaac cttgaaagat cgtctttgag gtcaattccc tcccagagtg acgacgagag
     1261 taagactcca cgagggtctg ctttgtgcgc ctctaggata tcatccacag tcaaatcatc
     1321 atcgtttttg ctatggcaga tccgaattcg ttttttgtta acttctgaga gattattgta
     1381 aatcatatag catctttcct ttgaggatgt aagaataagg ccgcgcttgt cttcgtggat
     1441 tttaaggagc gaatcgattg ttcgtatcac ttccatattg tcctcatttg atgttgcata
     1501 gttgagcgcc ttgacattta gaaagttgat ttttctattt tcaagcaaaa atggagattt
     1561 tggagtctca ataaaagcca cctcggagac tggaattcca gtattttcgc aaaaacttgc
     1621 aggctctatt gtagcagaca tgaatatcct aactggggag gtaagaaaag catccaagtg
     1681 ttttgatatg tccaatggct ttactgagac agagacaaaa ccaccatttt catccctttc
     1741 tggcttgttg attacaaagt tttctgcatt tgacttgatc tcatttttgg cgtgcaaaaa
     1801 tctctcgtat tttttctcta gcctctctaa cagctcgtag tttggtttca tttgaaatat
     1861 tctactctct aagagctctc ctgattgtct tgcataagac cgcgtcagat cttctatgag
     1921 cccaataacc atgttaatgt cagacaggtc atagtgttcc gcatttatcc cacactcctc
     1981 tagatttcct ttgtaaatat caaggccaac aaatccaatt atttgatctt caagcctatg
     2041 tgcctcatca aagatcgtaa ctgccctgct cagataatct tcaaaatatt tttgctggaa
     2101 ttttatcatc tgaaagaacg aataataatt ccatatggtg tgtggcgaaa ccagacctat
     2161 gtatttttgc agataataaa aacacgcatt ttcttgaatc ttgcctgact caatttcaga
     2221 tattgcaggc ttaaaattgc atttatccat tatttgttct ccacctttgg ttttcttcat
     2281 atcacagggc ccgatgtcgc atgtaagact gcgctttatg gcaaaatcag tttcctcaaa
     2341 atcgattttt ttagactcca taagctttag gcatgcgaaa tttgactcgc ctttgactgg
     2401 cttaaaaaaa ggcaagtcgt gcgaatactg gtcctgaaga tgttttgagg cagtcaccac
     2461 aaatgagcgg ccaaaatgct tggcaaccgc acctgcgata agcgatttgc caattccagt
     2521 tggtgcagaa agcagtaatg tgccaaatcc ctctgagagc cggtcttgga tttgccttag
     2581 cagatcaatt tggatttgcc ttggcttgaa gccatccgga aagtgatcca aaatgtttgc
     2641 cgaaggagtt gacacatttt gtttttccaa gatgacttcg gccagactct tttttgaact
     2701 ttggtaatac tctgaattgc tctgtttgat ttgctgagct tgatgtgcac ctaagttcct
     2761 ttttttaggt aagtgttgag tatttttgca atttttacat atcaagttac cctcttccga
     2821 ttttttggtt ttgattctag aaccacattt tgaacaaaaa agcatctgag tttttcaaat
     2881 ccccgtactt ttttcttttt ccattaacaa tgtattttgt agctgatctc attttatatt
     2941 ttgaggtatt ttcattgaaa catttaacaa tgaagtaatc gtgtaattgt tttgtgatcg
     3001 ctcctaaaaa attcatcatc gaccaacatg tcgaagacat ggaaatgcca gaaaacctca
     3061 agctaaacac gtttctcaaa gagtttcgcc aaaaatgcca taataaaaaa tgtgattttg
     3121 gcacatacgg cttttcattt ggccagtcac catttccagt tcccaacata gtgcaaaagg
     3181 ccttgataaa aaatgcaggt atgggtactt acgcaagcgt ttctggaatt ttacccctca
     3241 gagaagccat ctctgaatac aacaaatatt attttgatgt agatgttgat ccacgaagaa
     3301 ttcttatcgg aaacggcaca aagcaactaa tctacaacat cttggagatg ttacatggaa
     3361 ctgtaatcct tccaacgcca gcctggcttg gctatctacc ccagatcaga ttccttagga
     3421 aaaactttca catgctagcc acaagaagaa acaacggtaa gataatacca gaggagctaa
     3481 agcatctggc gcttcggcta cacgatagac aaaaaatcct atttttaaac aatccaaaca
     3541 atcccacagg tcgcctttat gaaaggttgg agcttgaaga gattgtggat atttgtagaa
     3601 tgtataatat cctagtgatc tcagacgaga tttatgcaca gacaacatat gactttaaga
     3661 aatttgtaag tatggcagaa atctatcccg agggtgcctt tgtaacaaac ggcctttcca
     3721 agtcacattc ctctggaggc tacaggctcg gatatgtcat tttaccgcac cattcatctg
     3781 agatattaca aaactttaaa aaaattcaag ctgcagaata tacatcagtt tcaacgccaa
     3841 tccagcatgc cgcggttgcc ggttttcagc caagcaaaga gataaacaaa tactttgaga
     3901 cgacaagaaa catccaccac atgatgagtg agtatgtgta cgaaagccta aatgaaatcg
     3961 atgatgtcag aacgacaaaa cccgaagcga cgttttatct tcttgcagat tttaatgcgt
     4021 tcacagatga ttttcaccgc cataaaatca taacatcaca acaattttcc gaatccataa
     4081 tcggtcatcc atatcacacg gcaattgtgg gtggagatag tcttgtattg gaaagaactg
     4141 acttttctgc aagaattgct tttgtcgatt ataatggcgc ttcaacatac cagaattacc
     4201 aaaaccacaa accaaaaaat caatcagaca gaacagaatt tgtaaaaaag aatgctcctc
     4261 gtatagttga cggcctagac atgatcaaaa aatattttgg cgatcttacc aaatcaaaga
     4321 tgcgcgtcaa gagcagagcc tcacattaac aatcgcgaat cagttcattt attgttttta
     4381 ctttcgtccg taactggaat attgaatttc tttgcgcgcc caagtgcaat cgtagttatt
     4441 attacagatg taagaatgat cacaaaaacg atttgtgggt atacctcgcc tttttgcaaa
     4501 cccattgtta atggtatggt tgccatcact gctgcggcaa gaccccttgg aatcattgcc
     4561 tgagtgactt ttttttcgaa tttgttgaat ctttttgtca ggattccctt taccacgcca
     4621 attcttgcta tgtagatcaa aattgcacca attacgccat aaaagacata ctctagctgg
     4681 gttatgcttg caagcaatcc tataaaaaca aagaaaaatg atcgtaccat gaaagttagc
     4741 tcatggtgga cggaattgtc tgatggtatt tctggaattt tgaatcgcag atacttggaa
     4801 agagtttgct tgttgccgag tatgagtcca aaaatcaatg gtgctagggc gccggtgcct
     4861 ccaaatgact tgtccaagaa aaatatgaca aacaatattc ctattgttag catgtatgcg
     4921 tgttgtgcct tttggagttt tgtcattgca aacatccaag gtattcctac tccaaagcca
     4981 agcacagttc caactgcaag ggatttggca aatcgctcta ttgcaagatc ctgatttaga
     5041 tagcctgcca ccaacacatc gattaggaca aaggcaatta ccgtgaccaa aatatcagta
     5101 actgcagagt ctaagctaag catagatttg gtttcttctg aaatgtttag ttgcttgaca
     5161 agtccaaata cgataattga gctgctgcca ccaactatcg tcccaagtaa aacactctcg
     5221 atgagtgacc agttaagacc gtaataggtc actactgcta cagatatgac agatgcagca
     5281 aagcccaaaa ttgaaagcgc cagcgcaaaa tgagctgtct tgatcacctt tcgaatatcc
     5341 aagcccagcc cgccgtcaaa catgattatt atcagcgcga ctgctgcaaa atacggcaca
     5401 atttgaacta tcacatcttg tggaatcact ccgaaaactg ggccaattat tacccccaag
     5461 accatcagaa atgcaatatc cggtattcca gttttcctaa agaatgcctc accaacaacc
     5521 cctaagaaaa tgataatagc tgcagacaaa agcaaagttg ttgccccaag ctcagccact
     5581 tcaggaaatg aaaactgcgg tattgcttgc tgcattttgt caagagagtc tccaattaat
     5641 gacgagatag ctatttcgac cattttatgt tcagaacaac aaaaattttg taaggttgtt
     5701 ttaaagtctg tttgtcttga taattagagc aattatgcgt aaaatttttt aatcacacaa
     5761 aaaaagacgc aatagtatga atctacagct tgcggcaatg gcggtttttg cagcattgtt
     5821 agtgtcagtt actttggcag acatctcgtt tgctcaaacg acaccaaaaa atgacataaa
     5881 atcaattatg gagaactacc gcaaggcaat tctaaaggcc caaactgatt ttcaggcagc
     5941 aattaaaaaa gcaaatgtcg atgcaaaaga tgtggtttta aaaggcctgc caattgacca
     6001 aatcaacgca gattccaaat ccaagataca aaaagcccga gtcgacctca aagcatcaat
     6061 agccgaggcg cgtgtagacg caaaaaatgc cctcctacag ctaaaggccg caatagacgc
     6121 aaaatcctca taacaattcc aatttcaggc tattttgttg ccatttttct gctttggcaa
     6181 actacacatt tgaggcccct agtaaagctg ccacattttt tgcatttgag ggaaaacgtg
     6241 tcgtattttt cgactttgct tatcttatag tatgccataa gaacgaccaa tagaatactg
     6301 gccataatca ctattggtac catattgaca ttgtttgcag acactttata gtggatgttg
     6361 taaatagagt ctgatgccac cgcgaatgcc ttgttttgaa tcaaaactaa agctgctacc
     6421 aaaaatgcca gacacgctct aaaattcaaa aaagattcag aaatccggct cacaattcag
     6481 taaacaatga ataaacttta acaatttgtt tgtctgaaaa agacaacggc cttttagcta
     6541 ccggatctgt tttttgagat catcaaatgc gtcttgtatt tcacttactg ctgcaccatc
     6601 ttctagggtg ctgacgtctc cgatcttttc gttacttgca attgccttta gaagcctcct
     6661 cattatcttt ccactgcgcg tttttggaag atttgataca aaatagatct gatgaggcgt
     6721 tgcaattgcg ccaattcctg ttcgaaccct attcaatacc tcctttctga gatcctcggt
     6781 taccgcaact ccttcttttg taaccaaaaa cgcaatgata acctcgcctt ttatctcatc
     6841 tggaactccg cagactgctg cttccgaaat ggagttgtgt gagacaagac tgctttcaag
     6901 ctcggcagtc ccaattctgt ggcctgcaac ctttagcacg tcgtctgcac gaccaaggag
     6961 ccagaagtat ccatctttgt ccttaattgc ataatcccct gtgtagtaaa atccgtcata
     7021 ttttgaccag taaacagact tgtatttttc atcatcaccc caaagcgtca aaagcattcc
     7081 aggccaaggt tttctgatga caagatagcc ctttgtatta tgcttcacct cggctccgtt
     7141 ttcatccacc acagccaaat ctacacctgg gattggaaaa gagccggagc ctggcttgag
     7201 tggcattgtt tcaaggcctg gaaggggaga gatcataatt cctcccgttt ctgtctgcca
     7261 ccatgtatca attattgggc agcggttttt gccaattgtt ctaaaatacc atttccacac
     7321 ttctgggttg attggttcgc caacggtacc caaaagtctc agactggaca gatcaaaaga
     7381 atttggaatc gtgtctccaa acttcataaa cattctaagt gctgttggcg ttgtgtaaaa
     7441 aatagtaact ttgtatcttt gtattatgtc ccaaattctt gccggagacg gatagtcagg
     7501 tgccccctca tacattacct gagttgcgcc acaaatcaga ggcccatatg ccacatagct
     7561 gtggcccgtc acccaaccaa tatctgccgt gcaataatat acatcagaat ctttaatgtc
     7621 aaatgcccac ctaaacgtag aactaatgtg cgttagatat ccccccgtat catgtagaac
     7681 cccttttggt ttgccagtgg ttccagaggt gtagagaata tacaatggat gatcgctatc
     7741 aagtggttct gcgtcacaaa catttgaagc attttttacg acatcttccc acatcaaatc
     7801 ctttttgttt aatgttattt gattgccgat acgtcttaaa acgattacat tttgtacaaa
     7861 atccaagttt tcaattgcca catcaacagt ttctttgagt ttgactatgc ccccccttct
     7921 ataaccccca tctgcagtga tgactatttt tgactgcgaa tcaagcgccc tgtcacgaat
     7981 cgatgttgca gaaaatccag aaaaaattac tgtgtgaatt gcccctattc ttgcacaggc
     8041 aagaattgct acaataatct ctggaaccat gggaagatag attgtaattc tatccccttt
     8101 ttttacccca actgatttta atgcgtttga aagcttttga acttcgtcga agagctcgcc
     8161 atatgtaatg gtgcgtcttt cttggttttc tccctcccag agaattgccg gctttagttt
     8221 ttgtttttgg tttacatcca gagcattgta cgaggcattt atttttccgc ctacaaacca
     8281 acgtgcaaaa ggaggggtcc attctagtac cttgctccac atctctgacc agactagttt
     8341 ttgtgcctgt ttttcccaaa atgacacata atctttggat gcctcaagcc tggtctttgc
     8401 atcattatta ccaagaccaa ttgtaaaaat ttcagaattc acaatagttt ttgattgttt
     8461 ccaaacttaa aattttttat aaaaaaagag gtttgtgctc cattaagctt ctagacattc
     8521 agacccggtg ttttcagaat gtgtgttgac aaaaccgcca ctttgttgag tcggcgtcac
     8581 ttgtggaatc gctgcatgcc attttggcgc gtccttattt ggctcggccg aggggggagc
     8641 tatgttggca gtaacgggtt cttgttgttc cgcatcagtg tttgtggttt tgtttgcatc
     8701 accagttgat tgtgttgcat cacttgtact aggaggcacg tttggagtct cgacctgctg
     8761 tcctgaggcc gtatttgtga caagataaga caccgccgat tgctggatct gtacttgttg
     8821 gttgacgtcg gttttgaccc catctagggc aatatcagca attcttcttt ggatgtttag
     8881 aatctccgct ttttcatgat caatgtgctc tattagttca cccgtgtgtg tcttgaccag
     8941 atcaaacttt gattcagaga tttcgttgct tttgaattga accttagcat caaacagcaa
     9001 tgtctttact agtttaagct gagcattcag ttcttcgagt cgtgcagcca gagttgtgct
     9061 gatttccttt tcggattctt caaggctcaa aagttttgac ttgtattttt cgtggatgat
     9121 ttctgcatct tctctcatct cgtcgttttc tgagactatg tcaattagtg ctttgagcct
     9181 tcgaattgtg agtccttttt ctcttaggaa tcgttgtgcg tcgagtctcc attttggtat
     9241 aaagatcacg acatcgcctt gaacaacaag ttgttcaaat gggacttgtt ttagtccgtc
     9301 aagaccacaa tcaattccca cagtctgtat ggaaccgtca atgtcagtta ttgttccgat
     9361 aactttgccc atataggtgc catacatgtc tttgacttgc ttgccgataa tctctatttc
     9421 gtccttgttc attagtatga cttttgtgtt gcatataacc aacaccatgt taacaagcct
     9481 tctttttttg gtaagaacaa tttaacaaaa caaaaacggg ttttcgaaat tttaaaatgc
     9541 gaaccaaaaa aacccacatt atgattgaca gagacggtct aaatcagata ttgagctttg
     9601 tttcaaagcg ctggacggat ctgagtcggg attctaacaa caaggcacta caaaacttgc
     9661 ctgccgattt ttggatgaat tccgttggtg gaaaggctgg taatggacat tggattacaa
     9721 tgctaatgta cacagaaaat gaaaacgacg caaatgcact aaaagaggtc cttttgcgat
     9781 ggcagacaaa gggaaaatca tcgacaacgg atctgattca gatcaatcga aaatagttta
     9841 tttacaatcc cccggttttg atttcattgg ctgaagaatt tacaggttca gaaaaagagg
     9901 ttttgctaaa acatttttcc aatgcagagg attccgtttt tgcaataatt acccccagac
     9961 aggtggatcg tggagccctc atgtcaagat acagcagaac tgacaagagc atgaggagaa
    10021 tctttcttga cgaattttta gcaaatgaaa gccgtggtga ggaattttat aacaaagtcc
    10081 taattgaata tggtgatgac tcagttgctg agctcggcat tgcacaaatc gcaatagaag
    10141 gaatatcaaa cattgcggta aaaaaaattg aagaccgaag aattggctta tcatatcttg
    10201 aaaaatcttc tcgctatgtg tcttgggata aaaaagtaaa tggacacttt aagttttatc
    10261 gcgagccgca aattatggac tcaaagtttg cgaatgcata tttggacagc tgcaacttgg
    10321 attttgaaat atattcaaaa aacatacagc caatgattga ttacgtcaga gaaaaagagc
    10381 caatagataa gctaaagttc aaagacactt tgtctggaac tgaggttttc ttctccaagc
    10441 taaaagatga tcgggacata aaatctgcaa ctatgattta taatgcgaca acaaaggcaa
    10501 aagcacttga tgtcatgcgg gggttgttgc ctgcatccac acttacaaac gtaggcataa
    10561 caggaaatgg caggtcattt gaatacctgc taaccatatt gttcagctca aatctagatg
    10621 aagagcaaaa tctcgcccat aaaatcaaac aagagttaga cactaccatc aagtcctttg
    10681 ttaggcgctc agatgacaag tacggcaagg cactacaaaa ctacttcaaa tcactgcaaa
    10741 agacatcatc caggctcgta aaaaaacacc cagtctccac aaagtccaaa ggcgcaactg
    10801 taaaactagt tgactatgaa ccggaattaa gggcgctaga caaaataatt gtagccattt
    10861 tgtatgaagg tcagacagtt acctataacg caatactctc aaatgtaaaa aagatgtcta
    10921 aatctgaaaa gacaaaaatc atcacctcct ttgcaaagct aagacaaaac aggaggcagc
    10981 gtccaccacg tgcctttgag atgacacatt atacatttga tattgtggga aattttggca
    11041 tgttccgcga cttgcatcgg cacagagttc taacacttga gcgacagcta ctcacaacag
    11101 atcatggata tgtcatcccc agagagattg cagagattgg tcttgaaaaa gaattcaaag
    11161 actgcatgga tgtatcaaaa cgagtttttg attccatgcg acgaaagatt ccacagcagg
    11221 cccaata
//
LOCUS       DASWKH010000094        11798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_241849, whole genome shotgun sequence.
ACCESSION   DASWKH010000094 DASWKH010000000
VERSION     DASWKH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11798
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241849"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VGA92_07260"
     CDS             <1..272
                     /locus_tag="VGA92_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846247.1"
                     /translation="LGKDLDDVFGLKIINDRKWKELAEIFYRRHIIVHNNGRSDSKYK
                     EKTGVEVGTDLSPELDYVTQTLDTFRYFSKLIRDFFKEKYSPVTP"
     gene            509..1792
                     /gene="aspS"
                     /locus_tag="VGA92_07265"
     CDS             509..1792
                     /gene="aspS"
                     /locus_tag="VGA92_07265"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX9846248.1"
                     /translation="MRFKKTHNIDELSLDNQGQQIVIGGWIEDLRKLGKLTFLTVRDV
                     TGRAQVIVKDENAIPEDMTRQSVVLVKGTIQSTKARDFTFEVKADDIDILTKAIHPLP
                     IDPIGRLESNIDNRLNARALDLRNQRVAAIFKIRHHVLASLRKMLAQKRFIEITTPKI
                     IGSASEGGANLFSLEYFGKKAYLAQSPQLYKEQLTVGLERVFEIASFYRAEKSHTGRH
                     LSEFTSVDIEAAFMDYTDVMDVLEELIVSVFKDVLESCKEELKSLEHILKTPKQPFEK
                     ITYKQALDELAKAGTSLNFGDDLLDSHLKIIGANHPGFFFLIDWPMSLKPFYIHEKDE
                     DPKLSRSFDLQYGYLELSSGGRRLHDPSKLKSRLAEQGLDPASFEDHLRSFEWGMPPH
                     SGWGMGLERLLTIIVGIDNIREVVLYPRDPERLFP"
     gene            1873..2154
                     /locus_tag="VGA92_07270"
     CDS             1873..2154
                     /locus_tag="VGA92_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846249.1"
                     /translation="MKAPTSFARYAESDSKGADITSMISGAVNRKQDQYAATASMIVC
                     EVWARFTHIRGDICEAICGVFATDVGTQSDYQIANPNSRCVEVEKGGNY"
     gene            2212..2553
                     /locus_tag="VGA92_07275"
     CDS             2212..2553
                     /locus_tag="VGA92_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846250.1"
                     /translation="MAVCEFCSSNFGDRKCYFCQKHVCTSCMTDDRTRCKKCYVNKAR
                     LGWKATLKRNKIILGFVAFVWFYAVFPGPLIPGLHSGYYVIFVIAAILCMIPLGLMLF
                     FWSRAPPASDV"
     gene            complement(2550..3566)
                     /locus_tag="VGA92_07280"
     CDS             complement(2550..3566)
                     /locus_tag="VGA92_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX9846251.1"
                     /translation="MYKISLITGDGIGPDLSESAITVLNTIHDRLGLKFDIKELMAGD
                     AALAKTGKALPDETLDAIKKSDACMKAPVGESAADVIVVLRRVLELYANIRPAKSYPN
                     MPALKDNIDLVIVRENTEDLYTGQEFEIADAAVAFRIISEKASRRIAKYAFETARQRG
                     GKKKVTCVHKSNVMRKTDGLFSRVCAAVAKDYPDIAFDQMYVDACAMNLIRRPETFDV
                     IVTTNLFGDILSDEAAEVVGGLGMAPAANIGDNFALFEPVHGAAFDIAGKGIANPSSF
                     ILSAKMMLDWLGAKHKDEKCLDVAKKLESAVYGVVKKGTKTRDIGGDKTTKEFTKEIV
                     SLLS"
     gene            complement(3581..5098)
                     /locus_tag="VGA92_07285"
     CDS             complement(3581..5098)
                     /locus_tag="VGA92_07285"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9846252.1"
                     /translation="MRVRIFDTTLRDGEQTPGVALSPEKKLAIAKKLDDLGVDAIEAG
                     FPIISEGEQQAIKLITKANLRSEICGLARTSKKDIDAAIDCGLNYIHTFIATSDIHLQ
                     YKLKMTREEVLAKAIEAVEYGKSHGLQVEFSAEDATRTDRVFLKHVFSEVAKAKADRI
                     DIPDTVGYSTPQYFAEITKDAIEASKLPVSVHCHNDFGLAVANAISGIQAGAQCAHVT
                     INGIGERAGNASLEELVMALQCLQFSAKWETGIKTKLLYETSRYVSNLVGIVVQPNKA
                     IVGENAFGHESGIHTHGILNNPLTYEPISPDLVGRTRWLQVGKHAGIHGISAMLEEYG
                     IKPNESQLRQILEKVKAIGDQGKQVTDVELLSIANEILGEHAIKRIAQLAGFSVSTGI
                     GNMPYAFVKINIDGADFIGTDYGVGPVDAALNAIQKITGEITKVRVKEYKLDSISGGS
                     DALCEVTIKVEDAYGNMASAKSIGEDIVITSVQAVMDGINRIMLKKTLKQRKIGS"
     gene            complement(5095..5580)
                     /gene="ilvN"
                     /locus_tag="VGA92_07290"
     CDS             complement(5095..5580)
                     /gene="ilvN"
                     /locus_tag="VGA92_07290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9846253.1"
                     /translation="MVWAILSILVENKPGILFKVTHLFRSKNFNIDAISVGVTQNPEV
                     SKMTITTIGDERQVDQIVKQLDKMIDTIEVKRLDDKKTVYRELVLFRVKVSKASDSME
                     INNIANAFAAKVHDARKDSVMIELTSTPDQINNLQELLKPYEILDIARTGVAALQRAG
                     E"
     gene            complement(5591..7279)
                     /gene="ilvB"
                     /locus_tag="VGA92_07295"
     CDS             complement(5591..7279)
                     /gene="ilvB"
                     /locus_tag="VGA92_07295"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX9846254.1"
                     /translation="METLSGARALIKALEKQGVKEIFGLPGGANLPMYDELYKSNIRH
                     ILARHEQSAAHMADGFGRVSRRAGVCFATSGPGATNIVTGLATAQADSAPMVAITGQV
                     PLGMIGRDAFQESDIIGIANPVVKYSYQPTKPSEIPEVVKKGFYIAETGRPGAVLIDI
                     PKDVQQNEASVDFPEEVKIRGYHPWTDPDMIAIEKAIKLLLSSSKPIILAGGGVIISS
                     AFAELQSLAEMLLIPVVTTFKGKGAFPENHVLSLGPIGMHGHAEANKIMTEADCVLAI
                     GTRFSDRSVGRFDEFEKHLKIIHLDVDPAEIGKNQTTSVAVVGDVRASLRIFLKLLMQ
                     KALKKSEHNVWLDHVKEVKEYWRQNLKLHPGELSAARILRKLREVLPHESIVTTEVGQ
                     HQMWASLFYDVIHPGTFFSSTGLGTMGWGFPAAIGAKTARPDLPVVDIAGDGSFNMTE
                     NSLATSVLDNLPVIVFLVNNYSLGMVAQWQRTFYDRRMIGVDQKHCPDYVKLAESYGA
                     QGIRVGNLEELGNAIKAGLKSDVATVIDIPIDPEEDVLPFVAPGTSLKDMILPS"
     gene            7487..7888
                     /locus_tag="VGA92_07300"
     CDS             7487..7888
                     /locus_tag="VGA92_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9846255.1"
                     /translation="MSDREEIISIIEMFFEIGKTKDLEPLRTIQLDEPSFSSFSDIPP
                     YDLKDYKTTVALEELRFVSISDYDYEIKNPKISIFGDAAVVAFELTQKGMLVDNKAFT
                     GEHISIDGRATFVLVKKPSWKIAHIHLSKIN"
     gene            complement(7914..9029)
                     /gene="lsrF"
                     /locus_tag="VGA92_07305"
     CDS             complement(7914..9029)
                     /gene="lsrF"
                     /locus_tag="VGA92_07305"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HEX9846256.1"
                     /translation="MFSNSLDMDWGLKNRIASILKPNNNRGVMLAVDHGYFLGPTEKL
                     EEPKKTIAPLVKYCDSLMVTRGVVRTSVDPNFPVPIVLRVSGGTSIIGEDLSNEKITT
                     SVKDAIRINATALAMSIFVGAKYEHNSLVNLGNLVNEAEEYGMPVLAVTAVGKELEKR
                     DARYLSLSCRIAAELGAHMVKTYYCDNFEKVVSSCPVPVIIAGGPKLPERDALALTVN
                     ALKSGAAGVDMGRNIWQSQYPIPMIRAVRSIVHSNYTLDQAYDLFKKLCQEYPIRNNQ
                     PNTSGNNFSQNQKPQQNGSQQNSPQQSRPQQTGPQQSQQMPNNPQQNRPQQPRPQQVR
                     PQQGQQKKPQQKPHQQKQPQKKPEAKPQPAAAQAKPQ"
     gene            complement(9049..10074)
                     /locus_tag="VGA92_07310"
     CDS             complement(9049..10074)
                     /locus_tag="VGA92_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HEX9846257.1"
                     /translation="MKAAFVKGPSIVLVQDSEQPMLGPGDILVKMQACGICGSDVEKV
                     FGKYGQPSMRLGHEPAGIILDMGSGVTDFKKGDRVFTHHHVPCYSCHYCKHGHETMCP
                     KYYETNLAPCGLAEEYVVPEWNVRHGGVLKIPDTMSFDEAAMIEPLACCVRAWAKFSY
                     NKGDTAAVFGAGPTGMMHVMLAFANEFSKVFCFDVNEFRLDFAKKFGAISLKSGQPES
                     VSKVLSETQNQGVDVSIVATGNLMAVSDAISATRKGGTIMLFGVPSKGATMDVDMSVV
                     YSKELTLTPSYAASDLDTKQSLHLISSGKVDVKRLITHRYSLLESQKAFEHAHTGQNA
                     MKIIIHS"
     gene            10203..10907
                     /locus_tag="VGA92_07315"
     CDS             10203..10907
                     /locus_tag="VGA92_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846258.1"
                     /translation="MSRWDNPSDGFEDVEESRLLKKFHDYAELEKKESERKRFLEVSD
                     KKRPSVWGNRFVLSAIIQGAIIIGLTVALILMQVAFSNVSLIEFLSLSLDGPAKWFFF
                     GYFMYITLVVTIAITAIFYNHLESNLGRPIRGNAKYLVWTQLVGMNVGGAAATILLIV
                     FGFIGAGFVSFISGEQSELQNIMDFVEIPIAGLALAFACGIISGGIAYIRTYTGQRGP
                     KLNKMSFDDSSKYDRL"
     gene            complement(10904..11200)
                     /locus_tag="VGA92_07320"
     CDS             complement(10904..11200)
                     /locus_tag="VGA92_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEX9846259.1"
                     /translation="MKIVTVGSKSFVTSFQLAGVQGVVVDSPQTAFSEIKKLADDQEV
                     GLVLVSDDISAPINTDLTKLRATKSKPLIFSLPSVGSEKKEVDYRKMLKTILGV"
     gene            11283..11579
                     /locus_tag="VGA92_07325"
     CDS             11283..11579
                     /locus_tag="VGA92_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846260.1"
                     /translation="MSQIDTAKNVYLFVHGRMDLQQKSIDALASKGFSKDKIISAVPN
                     QVGSIGDYMAMLWMPPNPDHIKIQKITKVDPAEPVGMIGLWKGVSKDDLFTIPL"
     gene            11662..>11798
                     /locus_tag="VGA92_07330"
     CDS             11662..>11798
                     /locus_tag="VGA92_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX9846261.1"
                     /translation="MTIELECSDYGFECNFKLEGQKSLSLLKKLRDHFEQEHGIDYSV
                     DV"
BASE COUNT         3115 a         2587 c         2460 g         3636 t
ORIGIN      
        1 atttgggaaa agaccttgat gatgtattcg gcttgaaaat aattaatgat aggaaatgga
       61 aggaactggc agagattttt tacagacgac atattatagt tcataataat ggacgatcag
      121 atagcaagta caaggaaaag actggggtag aagtaggaac ggatttatcg ccagaactag
      181 attatgtcac acaaacacta gatactttta gatattttag taaactgatt cgtgattttt
      241 tcaaggaaaa gtattcccca gttaccccct aattacatcg acccgacgat ttttgacagt
      301 cggaaaagcc gacatcaact tgacagaacc taaccgaata atgatcaacc atcaagtaaa
      361 cagaatccaa ttagagacac tgttcaatta gaataccacc acccgacagt taatgcgagg
      421 aacgctactc ccgtcaagtt aaaagtgccc acctatagat acatttaact ttgttttatt
      481 gtgaattcga tttgcggggc caacgaaaat gaggttcaaa aaaacacaca acattgacga
      541 gttatctttg gataatcagg gacagcaaat tgttattggt ggttggattg aagacctgcg
      601 aaagcttggc aaactaacgt tccttaccgt gcgtgatgtt acagggcgcg cccaagtgat
      661 tgttaaagat gaaaatgcca tcccagagga catgacaagg caaagtgtcg tattggtaaa
      721 aggcaccatt cagagcacaa aggctcgcga ttttacattt gaggtaaagg cagacgatat
      781 tgacatttta acaaaggcaa tacatccgct cccaatagac ccgattggca ggcttgaaag
      841 caacatagac aacagactaa atgctagggc cttggatctt agaaaccagc gagtcgctgc
      901 aatttttaaa attagacacc atgttttggc atcattgcga aaaatgcttg cacaaaaacg
      961 attcattgag attacaactc caaaaataat tggtagtgca agcgaaggtg gcgcaaatct
     1021 tttttcgcta gaatattttg gcaaaaaggc atatcttgcc caatccccac aactctacaa
     1081 agaacagctg acagttggtc ttgagcgggt ttttgagatt gcttcgtttt atcgtgcaga
     1141 aaaatctcac actggaagac acctttcaga gtttacaagc gtcgacattg aggcggcatt
     1201 tatggactat actgacgtca tggatgtttt agaagaattg attgtttctg tattcaagga
     1261 tgttttggaa agctgcaaag aagagttaaa atcacttgaa cacatcttaa agacgccaaa
     1321 acagccattt gagaaaatca catacaagca ggcactagac gagcttgcaa aggctggaac
     1381 aagcctaaac tttggcgatg acctgcttga ctcgcatcta aaaataattg gtgcaaacca
     1441 tcccggattt ttctttttga ttgactggcc tatgagctta aaaccatttt acattcatga
     1501 aaaagacgag gacccaaaac tttcaaggtc ctttgatttg cagtacgggt acttggagct
     1561 ttcatctggc ggtcgaaggt tgcacgatcc ttccaagcta aaaagccgac ttgctgaaca
     1621 agggctagac cctgcaagtt ttgaagatca cctcagatcg tttgagtggg gcatgcctcc
     1681 gcattctggc tgggggatgg gccttgagag gctactgaca ataattgtgg gaatcgataa
     1741 tatccgtgaa gttgtcttgt atccgcgtga cccggaaagg ttattcccat aaaaaagacg
     1801 gttcagtaag atgctggagg ttatatcata ataaccacgt tcttaaataa ttcaaagcac
     1861 attgcgatcg caatgaaagc acctacaagt tttgcccgat atgcggaaag tgattctaag
     1921 ggggcagaca tcacatctat gattagtggt gcagttaatc gcaaacaaga tcaatatgct
     1981 gctactgcca gcatgatagt ttgcgaggtg tgggctagat ttacccatat tcgaggcgac
     2041 atatgtgaag cgatatgtgg ggtttttgcc acggatgtag gaacgcaatc tgattaccaa
     2101 attgctaatc ctaatagtcg atgtgtcgaa gttgagaaag gagggaatta ttgaacatcc
     2161 catatcttaa cagaaccaaa aagataaatc caaaaaccat ctaacacacc attggccgtt
     2221 tgcgagtttt gcagctcaaa ctttggggac agaaaatgct acttttgcca aaaacatgtc
     2281 tgcacctcct gtatgactga cgacagaact cgctgcaaaa aatgctacgt caacaaggca
     2341 aggcttggct ggaaggccac gctcaaacga aacaagataa ttttgggctt tgtcgcattt
     2401 gtgtggtttt acgcagtatt ccccgggcct ttgattcctg gccttcattc tggatattat
     2461 gtgatttttg taattgccgc gattctttgt atgattcccc ttggcctaat gttgtttttt
     2521 tggtccagag ccccaccggc atcagacgtc tagctcagca gagaaacgat ttcttttgta
     2581 aattcttttg tggtcttgtc tccgccaata tcgcgcgttt tggttccttt ttttaccacg
     2641 ccatacacag ctgattctag cttttttgcc acatcaaggc atttttcgtc cttgtgcttt
     2701 gcaccaagcc agtcaagcat catttttgca gacagaataa acgaggacgg atttgcaatt
     2761 ccttttcctg caatgtcgaa tgccgcacca tgaactggct caaagagcgc aaaattatcc
     2821 cctatgtttg cagcaggcgc catcccaagc ccccctacca cctctgcagc ctcatcagac
     2881 aaaatgtctc caaagagatt tgttgtcact atcacatcaa atgtctcagg tcttctaatc
     2941 aagttcattg cgcatgcatc tacatacatt tgatcaaatg caatatcagg atagtctttt
     3001 gcaactgcgg cgcaaaccct tgaaaataat ccgtcggtct ttctcatcac gtttgatttg
     3061 tggacgcatg tcactttctt tttgccacct ctctgtcttg cagtctcaaa cgcatatttt
     3121 gcaattctcc ttgacgcctt ctccgaaatt attctgaatg caactgcggc gtctgcaatc
     3181 tcgaattcct gtcccgtgta tagatcttct gtgttttcac gcacgattac caagtcgata
     3241 ttgtccttga gtgcaggcat gttcgggtat gacttggcag gcctgatgtt tgcgtacaac
     3301 tcaagcactc tccgcaagac aacaattaca tctgctgccg actctccaac tggcgccttc
     3361 atgcatgcat ctgatttttt aattgcgtct agtgtctcat ctggaagcgc ctttccagtc
     3421 tttgcaagtg ccgcatcccc cgccatcaat tctttgatat caaacttgag tccaagcctg
     3481 tcatgtatgg tatttagtac tgttattgca gactcggaaa gatctgggcc tatgccatcg
     3541 ccagtaatta acgaaatctt gtacattttg cacttgcagc ctagctgcca atctttcttt
     3601 gttttagggt tttctttaac attattctat taatgccatc cattactgcc tgaacactgg
     3661 taatcacaat atcctcgcca attgattttg cagacgccat gttgccatac gcatcttcga
     3721 ctttgattgt tacctcacat aatgcgtcag agccgccaga aatagaatcg agcttgtatt
     3781 ctttgactcg cacctttgta atctcgccag tgattttttg gattgcgttg agtgcagcat
     3841 ctactggtcc aacaccataa tcagttccaa taaaatctgc cccgtcgatg tttattttga
     3901 caaatgcgta tggcatattt ccaattccag tagatactga gaaccctgca agctgcgcta
     3961 ttcttttgat tgcgtgctcg ccaagaatct catttgcaat cgatagcaac tcgacgtcag
     4021 ttacttgctt tccttgatct ccgattgcct ttaccttttc tagaatttgc cttagctgag
     4081 attcgtttgg ttttattcca tattcttcaa gcattgcaga aattccgtga attcctgcgt
     4141 gttttccaac ctgtagccac ctagtacgtc ctactaggtc tgggcttatt ggctcgtatg
     4201 taagtgggtt gtttagaatt ccgtgtgtat gtatcccaga ttcgtgccca aacgcatttt
     4261 cgccaacaat tgccttgttt ggctgaacta caatgcctac taggtttgat acgtatcttg
     4321 atgtctcgta gaggagtttt gtttttatgc cagtctccca ttttgctgaa aactgtagac
     4381 attgtaatgc catgacaagt tcttctaggg acgcgtttcc tgcacgctct ccaattccgt
     4441 taattgttac atgtgcacac tgcgctcctg cttgaattcc cgaaattgca tttgctactg
     4501 ccaacccaaa gtcattatga cagtgaacac ttactggtaa ttttgaggcc tcaattgcat
     4561 cctttgttat ctctgcaaag tattgaggcg tcgaataccc gacggtatct ggaatgtcga
     4621 tcctatctgc cttggctttt gccacttcgg aaaagacgtg ttttagaaac accctgtctg
     4681 tccttgtagc atcttcagct gaaaactcga cttggagtcc gtgggacttg ccatattcaa
     4741 ctgcctcgat tgcctttgca agcacttcct cccttgtcat tttgagtttg tactgtaggt
     4801 gaatatcaga tgttgcaatg aatgtgtgaa tgtagtttag gccgcaatcg attgcagcat
     4861 cgatgtcttt tttgctcgtt cttgcaagac cgcagatctc tgatcggagg tttgcctttg
     4921 tgattagctt gattgcttgt tgttctcctt cggatatgat tggaaatcct gcctcaattg
     4981 catccacccc aagatcatca agtttttttg caatcgcaag ttttttttct ggagatagtg
     5041 caacgccagg tgtctgttca ccgtccctta gcgtggtatc aaaaattcta actctcattc
     5101 cccagccctc tgtaatgccg caactcccgt tcttgcaata tctaagatct cgtatggctt
     5161 tagtaattcc tgcaaattat ttatttggtc aggcgtcgaa gtcaactcta tcataactga
     5221 gtcctttctt gcatcatgaa ctttggccgc aaatgcattt gcgatgttgt ttatttccat
     5281 gctgtctgag gcctttgaaa ctttaaccct gaaaagcacc aattctctat atacagtttt
     5341 tttgtcatct agtcgtttta cctctattgt gtcaatcatt ttgtcaagtt gctttacaat
     5401 ttgatctact tgcctttcat cgcctattgt ggtaattgtc atcttggaaa cctcaggatt
     5461 ttgtgtaacg ccaactgaga tggcatctat gttaaagttc ttggacctaa ataggtgagt
     5521 caccttaaag aggattccag gcttgttttc caccaatatt gagagaattg cccaaaccat
     5581 accctatcac ttatgatggt agtatcatat ccttaagtga cgttcccggc gccacaaatg
     5641 gtaagacgtc ttcttctgga tctattggaa tatcaattac tgtcgcaaca tcgcttttta
     5701 gccccgcctt gattgcatta ccaagctcct caaggttccc tacgcggatt ccttgtgcac
     5761 cgtatgattc tgcaagcttg acatagtctg ggcaatgttt ttgatcaacg ccaatcatcc
     5821 ttctgtcata aaatgtcctc tgccactgcg caaccatccc aagggaatag ttgtttacca
     5881 aaaacacgat tactggcaag ttgtccaaaa cagatgttgc aagcgagttt tcagtcatgt
     5941 taaagctccc atcacctgca atgtccacta ccggcaagtc aggtcgtgct gtctttgctc
     6001 cgattgctgc tgggaacccc cagcccatcg ttccaaggcc tgttgagcta aagaaggttc
     6061 ccggatgaat cacatcataa aagagcgagg cccacatttg gtgttgtccc acttcagttg
     6121 taacaattga ttcgtgtggt agcacctcac gtagtttgcg taagattctt gctgcggaaa
     6181 gctcacctgg atgtaacttt aggttttggc gccagtactc ctttacttct ttgacgtggt
     6241 ctaaccatac gttgtgttct gattttttga gcgccttttg catcaatagt tttagaaata
     6301 ttctaagcga tgcccttacg tctcccacaa ctgctacgct cgtggtttga ttctttccta
     6361 tttctgcagg atccacatcc aaatgaataa tcttgaggtg tttttcaaat tcatcaaatc
     6421 tcccaacgga tctatccgaa aacctagttc caattgccaa gacacagtct gcctcagtca
     6481 tgattttgtt tgcttctgca tgtccgtgca ttcctattgg gcctaacgac aatacgtgat
     6541 tttctgggaa tgcccccttt cctttgaatg tggtaactac tggaatcagc aacatttctg
     6601 caagcgattg taattccgca aatgctgaag aaattatcac accacccccc gccaaaatta
     6661 ttggtttgct tgatgatagt aacaatttga ttgccttttc gattgcaatc atgtctgggt
     6721 cagtccacgg gtggtatccc cttattttga cttcttctgg gaaatctacg cttgcctcgt
     6781 tttgttgcac atcttttgga atgtcgatta gaacagctcc cggtcttcca gtctctgcaa
     6841 tgtaaaatcc cttttttacg acctctggaa tttcggatgg ctttgttggt tggtaagaat
     6901 attttacaac tggatttgca attcctataa tgtcactttc ttgaaacgca tctcgtccaa
     6961 tcattccaag tggcacttgc cctgttattg ccaccattgg agcagagtct gcctgtgcag
     7021 tcgcaagacc agtcactatg tttgttgctc ctggcccaga tgttgcaaag cacacacctg
     7081 ccctcctact tactcgacca aatccgtctg ccatatgagc agcggactgc tcatgtcgag
     7141 ccaaaatgtg acgaatgtta cttttgtata actcatcata cattggaagg tttgcacctc
     7201 ccggtaaccc aaagatttcc tttacacctt gtttttccaa tgctttgatt aacgccctag
     7261 cgcctgacaa tgtttccata aattaatcac caaaaaagcg cgctgtatta taattttaca
     7321 gcaccagctc gactagcagc aatcccaaaa gtgtcttagt cgagccgttt ttcataacgt
     7381 tcactagcta aaaccaaaat taatgatata aagattactt aggcatggca cggccaaaca
     7441 ggcattgtca taaagcttaa agtcggattg gtttaagcaa agcgcattgt ccgacagaga
     7501 ggagataatt tctataattg agatgttctt tgagattggc aaaacaaaag acctagaacc
     7561 gctcagaaca atccagcttg acgagccaag tttttcaagc ttttctgaca tcccgccata
     7621 cgacctcaag gactacaaga caactgtagc actagaggag ctgaggtttg tcagcatatc
     7681 tgattatgat tatgaaataa aaaatccaaa gatcagcatc tttggcgatg ctgcagtcgt
     7741 tgcgtttgag ctgacgcaaa aaggaatgct agttgacaac aaggccttca ccggagagca
     7801 catctcaatt gatggtcgtg caacatttgt gctagtcaaa aagccaagct ggaaaattgc
     7861 tcacattcat ctttcaaaaa taaactgatc ctcggatcga aataattgag aatttactgt
     7921 ggttttgcct gagctgctgc tggctgtggt tttgcctctg gttttttctg tggttgtttt
     7981 tgttggtgtg gtttttgttg tggttttttt tgttggcctt gttgcggtct gacttgctgc
     8041 ggtctaggtt gctgaggcct gttttgctgt gggttgtttg gcatttgttg gctttgttgc
     8101 ggcccggttt gttgaggtct gctttgttgt gggctgtttt gctgagatcc gttttgttgt
     8161 ggtttttggt tttgtgaaaa gttgtttcca gatgtgtttg gttggttgtt tctaattggg
     8221 tattcttggc agagtttttt gaataaatcg tatgcttggt ccaaagtata gttagagtgg
     8281 actattgacc tgactgcgcg aatcattgga atcggatatt gtgattgcca gatatttctc
     8341 cccatgtcca cccctgcagc gccagacttg agtgcgttaa cagttagtgc aagtgcgtcc
     8401 cgttctggaa gctttggtcc gcctgcaatt attactggga ctgggcagct gcttactact
     8461 ttttcaaagt tgtcgcaata atatgtcttt accatgtgtg cgccaagttc tgctgcgatt
     8521 ctgcaagaaa gtgacaggta tcttgcatcg cgtttttcaa gctcctttcc aactgcagtt
     8581 actgccagga ctggcatgcc atactcttct gcctcattta ccaagtttcc aaggtttact
     8641 aacgagttgt gctcgtactt agctccgaca aaaattgaca ttgcaagagc tgttgcgtta
     8701 attctgattg cgtcttttac gctagttgtg attttttcat ttgataggtc ttcaccaatt
     8761 atgcttgttc cgccagatac tctaagaacg attggaactg gaaagtttgg atcaactgat
     8821 gttctgacaa cgcccctagt caccattaac gagtcgcaat attttacaag tggtgcgatt
     8881 gtttttttgg gctcttccag tttttcagtt ggccctaaaa agtaaccgtg atctactgca
     8941 agcatcacac ctcggttatt gtttggcttg agtatgcttg caattctatt ttttaaaccc
     9001 cagtccatgt ctaacgaatt tgaaaaaata aacccttctt aatcttttct agctgtgtat
     9061 gattatcttc atggcgtttt ggccagtgtg tgcgtgctca aacgcctttt gagactctaa
     9121 aagagaatat ctgtgcgtta tcagtctttt gacgtcgact ttgccagacg agataagatg
     9181 cagtgattgt tttgtgtcca gatctgatgc tgcatagctt ggagtaagtg tgagctcttt
     9241 agaatacact acgctcatat caacatccat tgttgcgccc tttgacggaa caccaaacag
     9301 cattatggtt ccaccttttc ttgtggcaga tatggcatca gagactgcca ttaggtttcc
     9361 tgttgcaaca attgatacat cgactccttg gttttgtgtc tctgagagca cctttgaaac
     9421 agactctggc tgtcctgact tgagagaaat tgcgccaaat tttttggcaa aatccaagcg
     9481 aaactcattt acatcaaagc agaatacttt ggaaaactcg tttgcaaatg caagcattac
     9541 atgcatcatt ccagtaggtc ctgcgccaaa cacagctgca gtatcaccct tattgtaaga
     9601 aaacttggcc caggccctaa cacagcatgc aagtggctct atcatggctg cctcatcaaa
     9661 tgacatcgta tcagggatct tgagaacgcc gccatgccgt acattccatt ctgggacgac
     9721 gtattcttct gccagaccac atggggcaag gtttgtctcg tagtattttg gacacattgt
     9781 ttcatgtccg tgtttgcagt aatggcaaga gtaacagggg acgtgatggt gtgtaaatac
     9841 tcgatcacct tttttaaaat cagttacgcc agaacccata tcaagtatta ttcctgccgg
     9901 ctcgtgtcca agcctcatgg atggttgtcc atattttcca aacacctttt cgacatcaga
     9961 gccgcaaatc ccacaggcct gcattttgac tagaatgtcg cctggtccga gcattggctg
    10021 ttcagaatcc tgaactaaga caattgatgg gccctttaca aatgccgcct tcatttacag
    10081 aacctacaaa gatcagacta taagtacatt cacggatttg agtaactagt tagaaatttc
    10141 taaacattcc gttcggatcg aacataaaat cgattattat gaaaatcaaa tctagaatat
    10201 tgatgtcgcg ttgggataat cctagtgatg gttttgagga tgtggaagaa tcacgcctat
    10261 tgaaaaaatt ccacgattat gcagagcttg aaaaaaagga atcggagcga aaaagatttc
    10321 ttgaggtctc cgacaaaaaa cgcccaagcg tttggggcaa cagatttgtt ctttctgcaa
    10381 taattcaggg cgccataatt atcggattaa cagtagcgtt gattttgatg caggttgcat
    10441 tctccaatgt tagtcttatc gagtttttgt cactttcatt agatgggcct gccaaatggt
    10501 tcttttttgg atattttatg tacataacgc tagttgttac aatcgcaatt actgcaattt
    10561 tttacaacca tctagaatct aatcttgggc ggcccatccg tggaaatgca aagtatcttg
    10621 tttggacaca acttgttgga atgaatgtag gtggtgccgc agcaacgatt cttttgatcg
    10681 tttttggatt catcggagca ggatttgtca gctttatttc aggtgagcag tccgagctgc
    10741 aaaacatcat ggattttgtc gagatcccaa ttgctggact tgcgttggcg tttgcgtgtg
    10801 gaataatatc aggtggaatt gcatacatca gaacatacac aggacaaaga gggccgaagc
    10861 ttaacaaaat gtcatttgat gattcgtcaa aatacgacag actctagaca cccagaatgg
    10921 tcttgagcat cttgcggtag tccacttcct ttttttcaga gccaacagat ggaagcgaga
    10981 agatcaatgg ctttgatttt gtcgcacgaa gttttgtaag atccgtattg atcggagcag
    11041 aaatatcgtc acttacgagg actaggccga cttcttggtc gtctgcaagt ttttttattt
    11101 ctgaaaatgc ggtctgtgga gaatctacta caactccttg gactccggcc agctgaaaac
    11161 ttgtaacaaa gcttttgctg cccacagtta cgatcttcat gatgtgggtt ttatttgtgt
    11221 aaatttaacc gtttttgagt tctaggaaac attgatttat cgttgaatat catagatatc
    11281 aaatgtcgca aatcgatacg gcaaagaacg tctacctttt tgttcacggt agaatggacc
    11341 tgcagcaaaa atcaattgat gcattggcct caaagggatt ttccaaggac aagataattt
    11401 cggcagtccc aaaccaggtt ggcagcatag gcgattatat ggcaatgctt tggatgcccc
    11461 caaatccgga tcacatcaag atccaaaaga taaccaaagt ggacccagca gaaccagtag
    11521 gaatgatcgg attgtggaaa ggtgtctcaa aagatgatct ctttaccatc ccgctctaaa
    11581 ttaccagatt tgaaaaccag tattagatga catagtcaag ccacaaaaaa gttataagga
    11641 aattacaaag gttcttagct gatgacaatc gagctagaat gcagtgatta cgggtttgaa
    11701 tgcaacttta agcttgaggg gcaaaaaagc ctctcgctac tcaaaaaact ccgtgaccac
    11761 tttgagcagg aacatggaat agattattcc gtggatgt
//
LOCUS       DASWKH010000095        21192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2112645, whole genome shotgun sequence.
ACCESSION   DASWKH010000095 DASWKH010000000
VERSION     DASWKH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 21192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21192
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112645"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1330)
                     /locus_tag="VGA92_07335"
     CDS             complement(392..1330)
                     /locus_tag="VGA92_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HEX9846262.1"
                     /translation="MWVEKYRPLKISDLVNQKEIVGSITSLIKNVSEMPHLLFSGSAG
                     VGKTTLALCLARQILGDRWREYTLELNASDERGINMVRERVKKFSRFAGLDTNIPFKL
                     IILDEADEMTSDAQTALRRIIEDTSKYCRFVLIANNISRIIEPLQSRCAVFKFTRISE
                     KEVIAHLKEIAKKEKIKVDEEGLKTIFDYTEGDLRHAINLLQAAASLGNITEDNVKSS
                     AGLTKTKDVDEVLKLALGGKIMDARNKMIELIKVYGMSESDFLRYLNEAIYKTKHDNL
                     VEISEIIAKYDYRILSGANPEIQLSALLAELSKLGK"
     gene            1471..1983
                     /locus_tag="VGA92_07340"
     CDS             1471..1983
                     /locus_tag="VGA92_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846263.1"
                     /translation="MKINELLQAYSIGYDTQDVKTVINHDFKINVSDVEVIGKQGEVL
                     NLPRWIAQTLESEKHAEIQESDMGIELTQSISKENFQGEELSHLEPDFYIKVIGYMSR
                     QSEKEYDKLQSLLKTLIRKRLGKLVPRANSMELTADTAKKLSIEERALYNIIHKNSTD
                     FVNQIFGDKK"
     gene            1980..4055
                     /locus_tag="VGA92_07345"
     CDS             1980..4055
                     /locus_tag="VGA92_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HEX9846264.1"
                     /translation="MTLETQSRGALIDQVQSFLASFKDKSGSFRYVEEIDQMMAKQAK
                     YIVVDYNDIVSQKDIEVKFNVEPDEILYAFARAVKNILEERFPDYAKKISDDIRVRIA
                     NYPMQRSLRQINAEVIGKLTSVSGMVVRASEVKPLAKNVVYKCPEGHLTEVPLERGLN
                     IYTPSKCSHEKCAHRDMRIDSEQSKFIDFQIVRLQELPEDLPPGQLPHYIDVTIRQDL
                     VDNARPGDRIILTGMVRIEQEQITGTRIHSGLHRLRIEGNNIEFLGGRGSKNSRRSER
                     EEISPEEEKLIKSLARSPDVYERLVNSFAPHIQGHSLIKESILLLMVGSSQRVLQDGT
                     KIRGDINIFLVGDPGTAKSEMLKFCARIAPRGLYTSGRGSTAAGLTAAVVRDKTGIMM
                     LEAGAVVLGDQGLVAIDEFDKMKPEDRSALHEVMEQQSASIAKGGIVATLNARTSILA
                     AANPMYGKYDPFKNITENVALPIPLLTRFDLIFVVRDIPSKERDRIIAQHIINLHKRS
                     SPDNRSIVDIDIFTKYLAYCKRNDPVLTQDAEEKILEYYMKMRNVESEEMITVTPRQL
                     GGLIRLATARARLLMKDQVDGEDAERAIYLIQSMLEDAGVDVNTGKVDLGVLQGRPHS
                     EVSKLQLFMDVLKSLEGDHKTPVEEKLFVKELAKTGKFTEEEARNFIRRMLREASIYE
                     SKPGHYNRV"
     gene            4052..6178
                     /locus_tag="VGA92_07350"
     CDS             4052..6178
                     /locus_tag="VGA92_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9846265.1"
                     /translation="MKIAELELADTAKQFFAEQGITELFPPQKDAIDAGLLDGQSVLV
                     AAPTASGKTLIAMLAIMNFLKNKEGKVVYLTPLRALASEKFYEFKKLEKISLGRTIKV
                     TISTGDFDSVDKEAENADIIVLTNEKMDSLMRHSAEWIDKIGLVVADEVHLIGDQDRG
                     PTLEVLLTRLKEMETKPQILALSATVTNSDELSEWLGCALVLSDWRPVPLTEGVFDGG
                     TILWNNGESVEIESSIRGPPVDLCIDTIKNGGQSLIFAETRTRSASLAAKGADAVSKF
                     LSDPERKYLDEISKKLLDSNEHTELVKTLAALLKKGVGFHHAGLNQNCRQIIETEFRG
                     GKIKLLASTPTLAAGVNLPAQRVVISNIARYDVKSATNKPISILEYKQLCGRAGRPQY
                     DSFGEAIIVGNANAADLIDYYVNGSPEPIESQLTDDKALRIHILSHIVSNPGIKNEEI
                     FEFFSKTLAGLQTRKNTLRFSIEIAKKFLLRENLIVQKGERFASTEFGKKVSMLYVDP
                     LTAIYFRRGIEMASENKKHTLGFLHLISSCEEFFPKFSLRNRDYETVNVLLENHSSEL
                     VEPISEYDCSRSLLALHSWISESSEIQLSDNLNIESGDMHRLVEMADWLSYCLYELAK
                     LLGRSDLLEDLAIIRKRLLYGIKEELADLVQIKGIGRVRARKLYGRGIKTIDDLKKIP
                     VDKLAEIDKIGPTLADNIKSQLKKVR"
     gene            6179..7195
                     /locus_tag="VGA92_07355"
     CDS             6179..7195
                     /locus_tag="VGA92_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-1-phosphate transferase"
                     /protein_id="HEX9846266.1"
                     /translation="MQAEIISAIVASLIAFFVVLGLVPPLIKQLTKRNWVVKDVNRIG
                     GAMIPRPGGPAILTGIILSEISLYFLLPADELITKGIAAILVTTSAAFVVGFVDDRMV
                     LGGWFKPVALAVAAIPIIALGAYDSNLEFPLFGAVKIPALYLGVIIFMIPITGNTINS
                     IDVMNGIASGFMTIAAFSLAIALFILQNYSVAIMTLPLAFASLAFYKYHRFPSRIFPG
                     DSGALVLGAMYGVIAIIGHVEVIAAIALLPAVINSFLFLSSVKRIVEHRQIKNPTTHT
                     DDHKITTTKETDAPITLVRLIVAGKPLTEKEIGLTIFRLAVFSGILAIITAFLMEVKT
                     WVTL"
     gene            7180..7416
                     /locus_tag="VGA92_07360"
     CDS             7180..7416
                     /locus_tag="VGA92_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846267.1"
                     /translation="MGHSLIYIFIAAMWALILIGGAVLVTLVAQISVEGYGEQLDFLI
                     GSIIKAAIAIILVVIWVLVLTKMKNKIFQKQIKS"
     gene            complement(7413..7886)
                     /locus_tag="VGA92_07365"
     CDS             complement(7413..7886)
                     /locus_tag="VGA92_07365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9846268.1"
                     /translation="MITKFVSKKAKLGKNVKIWHFAYVGDKTIIGNNVKIGSLAHVDY
                     NVTIGDNTMIEGLVYIPPLSKIGKNVFIGPAAALTNDPYPPSGKMVGVTIKDGAVIGS
                     RAVIKAGVTIGKNSVVAMGAVVTKDVPDNTVVAGVPAKARYSREEYDKKQKKWQA"
     gene            complement(7893..8162)
                     /locus_tag="VGA92_07370"
     CDS             complement(7893..8162)
                     /locus_tag="VGA92_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9846269.1"
                     /translation="MNKKMMDILVCPIDKQFPLEIIETESKDQIVIEGALYCSKCSRF
                     YPIIEEIPIMLPDELRDKKQDIDFLEKNKEDLPQKIIKHANPWHL"
     gene            complement(8159..9091)
                     /locus_tag="VGA92_07375"
     CDS             complement(8159..9091)
                     /locus_tag="VGA92_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9846270.1"
                     /translation="MKIAQIGVGGWGKNHVRVLSQLGVLSAICDADQNRAKETGQKYG
                     VNYYTSLDAMLEENVDAVFVCTPTATHFEIATKIIQKKKSVFVEKPMTYDSEEGTRLL
                     ELAKKNGVLLTCGYIERFNPAVEGVKDFVKSKKYGDLIMLEFHRENRMPLHIKDVGII
                     YDTSVHDIDTAMWLFDDTPEVVFARAGKIRHDHEDFATIMLGFKDNKVAIISSNWITS
                     ARIRHFNAVCTEGIVSGDFLSQEVKIQKDQQVETLTKEKQEPLTLEIKSFLDALEGKV
                     NLRVRPEDAVNVTKIAEAALLSSKKGVPIYLDLK"
     gene            complement(9093..10010)
                     /locus_tag="VGA92_07380"
     CDS             complement(9093..10010)
                     /locus_tag="VGA92_07380"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX9846271.1"
                     /translation="MRLNVSKVVWFDGKFTTLEKAKVPITTHAIHYGTSIFEGIRAYW
                     NSKNLYIFRLDDHIKRFRRSGQFYSIALNYSDEQLAYAITNLCKKNNAKKSCYIRPFY
                     FVGDYGINLHVNETVPTHVAAFMFIFGDLFNKNGISAGISSWRKFSDASTPTLAKMGG
                     NYLNSILATQECKRNGYDEAILLDLHANISEAPGENIFMVKNDILLTPPLESSALEGI
                     TRDSVMKLAEDLGYQVKEKEISRSQIYLADEVFLTGTAAEITPIMSIDGKKIGSGKVG
                     KVTKNMMSAYMDVVMAKNEKYFEWLTPVY"
     gene            complement(10038..10640)
                     /locus_tag="VGA92_07385"
     CDS             complement(10038..10640)
                     /locus_tag="VGA92_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846272.1"
                     /translation="MTVAIYLAHLNPVTNAHVEIIEELKKDAKVKVMPVVFLDDGIEV
                     NSRSFPFSFETRKKMLTAVFGNSIEVSSNYTFYAPFFKYFPPILSPYSWKLRSQILEG
                     INEDFYTYTGDKTEGLMLKAYRLNPKVGTRKKVSASSVKNKLYDAAMGKESTWKEDVP
                     KQVVKIIEESWDVVKKYASAPDATTRIAGMKFPKAGHKTR"
     gene            complement(10686..10759)
                     /locus_tag="VGA92_07390"
     tRNA            complement(10686..10759)
                     /locus_tag="VGA92_07390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10723..10725),aa:Pro,seq:cgg)
     gene            complement(10804..11646)
                     /locus_tag="VGA92_07395"
     CDS             complement(10804..11646)
                     /locus_tag="VGA92_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9846273.1"
                     /translation="MVVENQATITYIQLLKEDLSIYRLVPNDGVIPEYKAGQFITIGM
                     HVPSEGKVIRRAYSIASHPENKKYVELVIRWVRKPLPGRLTTQLFNAKEGDELTWIKP
                     TGAALLINDKLPDGQKDERRIVCLGGGTGIAPFVSFAQHLHSTGDKREIVVLHGASYI
                     DELSYKDLFTDLEMESLDRGRDKWNFKYRAAISRPQEWFNRSWSGQAGRVETFLRTKD
                     GDPSPLEQLIGEKVTPQNTMFYICGWQGTIDGCMDYLSAKGFVTERNKRPDGSFEVKF
                     ESYG"
     gene            11775..11848
                     /locus_tag="VGA92_07400"
     tRNA            11775..11848
                     /locus_tag="VGA92_07400"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11809..11811,aa:Ala,seq:ggc)
     gene            complement(11850..12401)
                     /locus_tag="VGA92_07405"
     CDS             complement(11850..12401)
                     /locus_tag="VGA92_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9846274.1"
                     /translation="MASFEELKKIIESGSKQEKIDSLELEIDAKDPKILNLIISELDD
                     KDIEVRGEAFSTLMLNENDISEILIEGLKNQNKNIRGYSVLILANRNDKKAIAKIVEL
                     TSDESAMVRSCAVGALGHLKAGQAYLAIRRCLDDSNLEVKKSAIKSAIDIGDKNLLEK
                     LEELSKENDPEIEKLLVLAKNNL"
     gene            12501..12788
                     /locus_tag="VGA92_07410"
     CDS             12501..12788
                     /locus_tag="VGA92_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9846275.1"
                     /translation="MSSENRDTIFIGKKPLMAYVTSTLIQLANLPAVYIKARGLSIGR
                     AVDVAQIISRKTENAGYSIGQIKIGSEQLESKDGKQRNVSTIEIEVKRNTP"
     gene            complement(12815..13651)
                     /locus_tag="VGA92_07415"
     CDS             complement(12815..13651)
                     /locus_tag="VGA92_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX9846276.1"
                     /translation="MNDPSKPLVIPGVYDAIGAKIAQKVGFEAMFQTGYGTSATLFGM
                     PDYGFIGSTETVENARRICRAVSVPVIVDSDTGYGNALSVWKLVNELQAAGAAGIFLE
                     DQRWPKRCGHMAGKEVIEKEEYAEKLQAAVDAKKGKDFIIVARTDARATRGLDEAIER
                     AKYYKKIGADAVFVEAPKSIAEMKKIGKSINAPLVANMIEGGATPIISAKELHKMGFK
                     IILYPLSVLYANTFASLQILKELRKSGTTAKLKKNVVSFDEFNEIVELSKFRKLELRY
                     KQ"
     gene            13800..14573
                     /locus_tag="VGA92_07420"
     CDS             13800..14573
                     /locus_tag="VGA92_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846277.1"
                     /translation="MTKILLQLVIAAGILSSVFVLTTAYAEICSRPCEAPTIGVLDNG
                     QRIVENGLVISGRAFDVENQIQTIPTMTLNTGTLARIKLMVYDNDGVDALRTASITIS
                     DYQDDKNRKDMARISFNQAFTGVQTVDVMDKGGLLKDASATATQLDDFRTEVVFSFRP
                     VKPFDTSALIVDLWDQDQSARTNVFLGAVKTTGKEMVDFVPGMPSQVPAPLKQVEDGT
                     APENVECRAGFELVIRTTGAPACVYPFTAEILRSWGMVA"
     gene            complement(14615..15121)
                     /locus_tag="VGA92_07425"
     CDS             complement(14615..15121)
                     /locus_tag="VGA92_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9846278.1"
                     /translation="MTSQWFQRVGSSIPRGFSRYYILELLATKPYTGKEIIDSATEQS
                     EGKWKPSPGLIYPLLGRLLDEELIEETKDGKYQITKKGRATADDLEAINKIIKNQLEV
                     LMKVKNVGRFVVMDIIERMNMMGTLLSSNVSKMTKEEMQKYRKFLQSELQKLDNQESK
                     AGKEIKIE"
     gene            15226..16938
                     /locus_tag="VGA92_07430"
     CDS             15226..16938
                     /locus_tag="VGA92_07430"
                     /EC_number="1.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HEX9846279.1"
                     /translation="MVDSIDYDLIIVGSGLAGLRAAIEAAKTSPSLRIGVISKVQVMR
                     SHSVSAEGGTAAVLYEEEGDNIESHIYDTVKGSDFLGDQDVAERMCKEMPKEIYQLEH
                     WGMPWSRRKDGRVDQRNFGGYSFPRATFASDKVGFFLMQTLYDTCQKFDNIEFLNEWF
                     VTSIIHDGQQFMGITAIELSSGTFYVIKAKSLIIASGGAGRLYSFATYAYSSTPDGLD
                     MAFRAGLALKDMEFVQFHPTGILPSGILITEAARGEGGYLLNNNDERFMKKYAPGKME
                     LAPRDIVSRSMMTEIREGRGFKHETGVDCLKLDLRHVGDARIREKLGAIREISIKFSG
                     IDPSKEPLDVRPVCHYMMGGIHTNIDGATELKGVWASGEAACNSVHGANRLGANSTSE
                     CIVWGKITGDLAAKHALNAKSTSQFPHHLVTLEEKRIYDGIFRGQGEYNPYEIRQELT
                     DTMNDRAYVYRTETDLIEGLKKLRELKEKTWKHVDDKAKEYNTNFSNVMELDSMFRVA
                     EVVLVGAINRRESRGAHARLDYPQRDDANFLHHTLAYYDPKEPIMKKHPVTITRYQPV
                     ERKY"
     gene            16939..17370
                     /locus_tag="VGA92_07435"
     CDS             16939..17370
                     /locus_tag="VGA92_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX9846280.1"
                     /translation="MKRNENKEGIKGMANPGRYGIERVAYWLMRITGLGLLAYLIGHI
                     YETSSILNGREAWNAMLDLTQTTEGHIILTLVIGMCVFHTVNGIRVMLGHGGVGVGKP
                     ARPDYPYVPASQNNMHKLCIYASIGLAALAMMYGLSVLLGE"
     gene            17373..17717
                     /locus_tag="VGA92_07440"
     CDS             17373..17717
                     /locus_tag="VGA92_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX9846281.1"
                     /translation="MRESIIMKIHYGTALGAVVLVAVHILFRLTMDYGDSLQYENVIA
                     NYEFIPYAIMLELILILLSVHGFNGLRVILLELKQGRSYEKAVTYGCLAAMAILIAYG
                     SRTILMASMGMT"
     gene            17718..18488
                     /locus_tag="VGA92_07445"
     CDS             17718..18488
                     /locus_tag="VGA92_07445"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX9846282.1"
                     /translation="MSEVSQNMAEEKSSTEVSSSKTITLRIAKYNPEHDATQQFAEFK
                     IPYERWTTVLDAILDVKRHLDHSVAVRYSCRQASCGSCGMKINGRPRLACFTKISELN
                     SDVITVEPMSNFPIVRDLVVDFKRMFANHKKMMPFVIREDSEVTGNTKEFSQSPEELE
                     QYIQFANCIKCGLCNSACPTMATDSSFLGPQALAQSYRYVADNRDKGKNKRLKIIDES
                     HGIWRCHFAGSCSQVCPKGVDPAMGIQLLRGYMLGFRK"
     gene            complement(18489..18812)
                     /locus_tag="VGA92_07450"
     CDS             complement(18489..18812)
                     /locus_tag="VGA92_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX9846283.1"
                     /translation="MTQDLQELRRKIFDELSGIVDPEINTPITELELIDNVDINDSAV
                     KVDLHLTSPFCPAVFGFKICQDVHDNLLKIDGIDDVKVNVSNHFMAEAINNQVNNSPN
                     PKKQA"
     gene            18862..20319
                     /gene="argH"
                     /locus_tag="VGA92_07455"
     CDS             18862..20319
                     /gene="argH"
                     /locus_tag="VGA92_07455"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX9846284.1"
                     /translation="MYRSRLDKSLDKHTLDYVSSIHDDLQIAQYDIIGSQAHTIMLYE
                     NKILNKAETKKILLALEKIKKEKFVSSDSEDIHELIESLVIKKTGLKTGGKMHTARSR
                     NDQVALDLRMKIRDDINTICKCILDFIETLASVAQKHADTAMPLYTHLQQAQIGTFSH
                     FVLSYIDVLFRDMERFYVTYGRINESPLGAGPVGGTSITIDRNSTAKMLGFKGLVENS
                     IDATSSRDVVAEFVSDVAILMTNLSRMAEDIIIWSTSEFSFVELADKFSSPSSVMPQK
                     KNPDLLELTRGKTARVVGNLVAILTTVKGLASGYGRDLQEIKPSVWSSSRIAISSLVV
                     LNAMFATLKVNKQKMKKVASSGYLVALDVAEALVKQGIPFRTAHKIVGQLVQGAHQLG
                     KSLSELTLGEIKKSVKEKGADPKKLLKIISSTNTQTSLKGRTSQGSSGFSEQKRMIAN
                     RKSKAQAYKSGITKRTNEVSTAFRGLSTKVSSLVK"
     gene            complement(20320..20393)
                     /locus_tag="VGA92_07460"
     tRNA            complement(20320..20393)
                     /locus_tag="VGA92_07460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20357..20359),aa:Lys,seq:ttt)
     gene            20552..20626
                     /locus_tag="VGA92_07465"
     tRNA            20552..20626
                     /locus_tag="VGA92_07465"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20588..20590,aa:Asp,seq:gtc)
     gene            complement(20628..>21192)
                     /locus_tag="VGA92_07470"
     CDS             complement(20628..>21192)
                     /locus_tag="VGA92_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9846285.1"
                     /translation="GFVIMDAIDGSANAVRGIPFFCCSLAFATENKLSSITAGVVTDL
                     SSGDMYWATKGKGAFCNKKKIQVHKEKPIYKIVGINVSGATLQLIKKIQPLFENSNHS
                     RHLGANALELALFARGMIDVYVDLREKIRVTDMAAGYLIAKEAGGIILDQNLKPLDSD
                     LEYETRLSFIAAASQEILSEIKNQVKL"
BASE COUNT         6352 a         4344 c         4324 g         6172 t
ORIGIN      
        1 aacagtttta agacttcaac ttaaaacgat cagatttttc atcacaaaat ctcaattttc
       61 attaggaatc aattcgaacc gcattgttat tatcccctcc ctaacatgag cccactgaaa
      121 tttgaaccgt tttagacaga aatccaatca caaacgaata aaattagtac aatattgttg
      181 acaatcccac tagattctat gtaaacaaaa cctgaagaat cgctaaattc atagtgccat
      241 ttgatggcct acaataatct ctatagtata attatgccta atcatcagct aattatttca
      301 tatgacatct aacggttgaa tgtaaacatc tgtttaatga taattttttc ttcattgtgg
      361 actgacaaaa tctttattta cgctctctgc gctattttcc aagcttgctc agctcagcca
      421 aaagcgctga aagctgaatt tcgggatttg ctccagacaa aatacgataa tcatattttg
      481 caattatttc agaaatttct acaagattgt catgttttgt cttgtaaatt gcttcattaa
      541 gatatcttag aaaatcagac tctgacattc cgtacacttt gataagctct atcattttgt
      601 ttcttgcatc catgattttg ccacctagtg caagcttgag aacttcgtct acgtcttttg
      661 tttttgtaag accagctgag gacttgacat tatcttctgt gatgtttcca agactagctg
      721 ctgcttgcaa aagattgatg gcgtgtcgaa gatccccctc tgtgtaatca aagattgttt
      781 tgaggccttc ttcgtctacc tttatctttt ctttttttgc aatttctttt agatgtgcta
      841 tgacttcttt ttcggaaatt cttgtgaact tgaatacggc acacctactt tgaagtggct
      901 cgatgattct tgaaatgttg tttgcaatca gaacaaagcg gcaatatttt gaggtgtcct
      961 caattattct tcgcagggca gtctgggcat cagaggtcat ctcatctgcc tcatcgagga
     1021 ttatgagctt aaatgggatg tttgtatcaa ggcccgcaaa ccttgaaaac tttttgaccc
     1081 gttctctgac catgtttata ccacgttcgt ctgaggcgtt tagctctagt gtgtattcac
     1141 gccatctgtc ccctaggatc tgacgagcca aacacaaggc aagtgtagtt ttcccgacac
     1201 ctgcagaacc agaaaacaaa aggtgtggca tctcggagac gttttttatt aacgacgtga
     1261 tgcttcctac tatctctttt tgatttacta gatcggatat ttttaggggt cggtactttt
     1321 caacccacat agtatttgaa acagacaatg tgatgagttt ccgactgatc ttactaatac
     1381 attacggctg aggatcaaat gatcttagct atttgtaaac acatttgatc gatcgtatcc
     1441 attaaatcct tgaagcaagt ttggtaaatg ttgaaaataa acgagctttt gcaggcttat
     1501 tctatagggt atgatactca ggacgtaaag actgtcatta atcatgattt taagataaat
     1561 gtctcagacg ttgaggtcat tggaaaacaa ggcgaggttc ttaaccttcc tcgatggatc
     1621 gcacaaacac ttgaatcaga aaaacatgca gagatacaag aatctgacat gggaattgag
     1681 cttactcaat cgatctcaaa ggagaacttt caaggcgaag aactgtctca ccttgagccg
     1741 gacttttaca tcaaagtgat tggctatatg tcaagacagt ccgaaaaaga atacgacaag
     1801 cttcaaagct tactaaagac tttgataaga aaaagactag gaaaactagt ccctcgggca
     1861 aattcaatgg agcttactgc tgatactgca aaaaagctat caatagagga acgtgcgttg
     1921 tataacataa tacacaaaaa cagcacggac tttgtcaacc agatatttgg tgataaaaag
     1981 tgacacttga aactcaatca cgtggggcgc taattgatca ggtgcaaagt tttcttgcat
     2041 cctttaagga caaatctggc tcattcagat atgtagaaga aattgatcaa atgatggcaa
     2101 aacaagccaa gtatattgtt gtagactata acgacatagt atcacaaaaa gacattgagg
     2161 taaaatttaa cgttgagcca gatgagattc tttacgcatt tgcacgtgca gtaaaaaata
     2221 ttttagaaga aagattccca gactatgcaa agaaaatctc cgatgatatc agagtcagaa
     2281 ttgcaaacta tccaatgcaa cgcagccttc gacaaataaa tgccgaagta ataggcaagc
     2341 taacgagtgt ttcaggcatg gttgtgcgtg cctctgaagt aaaaccactt gcaaaaaatg
     2401 ttgtctacaa gtgcccggaa ggacatctca ccgaggttcc gctagaaagg ggcttgaata
     2461 tctacacccc aagcaagtgt tcacacgaaa aatgtgctca ccgtgatatg cgaattgact
     2521 ctgaacaaag taaatttatc gactttcaaa ttgtaagact acaagagtta cccgaagacc
     2581 ttccaccagg acaactacca cattacatcg atgtaacaat aaggcaagac cttgtggata
     2641 atgcacgacc aggagacaga ataattctta ctggtatggt aaggattgag caggagcaaa
     2701 ttactggtac tcgtattcac agcggccttc acagacttcg aatagaggga aacaatattg
     2761 aattccttgg aggaagggga tccaaaaact ctaggagatc cgaaagagaa gaaatctcac
     2821 cagaagagga aaaattaatc aaatctttgg caaggagtcc agatgtctac gaacgattgg
     2881 tcaattcttt tgccccccac attcagggac actcgttaat caaagagtca attcttttgc
     2941 ttatggttgg ttcttcacaa agagtattgc aggatggcac aaagattagg ggagacatta
     3001 acatcttcct ggtaggtgat cctgggactg ccaagagcga aatgctcaag ttttgtgcaa
     3061 ggattgcacc tagaggactg tacacctctg ggaggggctc caccgcagca gggctcacag
     3121 cggcagtagt tcgcgacaaa accggaatta tgatgcttga agctggagcc gtagttttgg
     3181 gtgatcaagg cctcgtcgct atagacgaat ttgataaaat gaagccagaa gaccgaagcg
     3241 cactacacga agtaatggaa caacaatctg caagcatcgc aaaaggtggt atagttgcta
     3301 ccctaaacgc aagaacatcg attttagctg cagcaaaccc gatgtatgga aaatatgatc
     3361 cgttcaaaaa catcacagaa aatgttgcgt taccaattcc acttctcaca agatttgacc
     3421 tgatctttgt tgtgcgagat attccatcaa aggaaagaga taggatcatt gcacaacaca
     3481 tcataaatct acacaagcgc tcatctcctg ataatagatc aatcgttgat attgacattt
     3541 ttacaaaata tctggcatac tgcaaaagaa atgatcccgt ccttacccaa gatgcggaag
     3601 aaaaaattct agaatattac atgaagatga gaaatgtaga atcagaggaa atgatcacag
     3661 taacaccaag acagcttgga ggactgatca gactagctac tgcaagggca aggcttttga
     3721 tgaaggatca ggttgatggc gaggatgctg agcgggcaat atatctaatt caaagcatgc
     3781 ttgaggacgc aggagttgac gtaaacactg gcaaagttga tcttggcgta cttcagggaa
     3841 ggccacacag cgaagtctca aaactacaac tattcatgga tgttctaaaa tcacttgaag
     3901 gagaccacaa aacacctgtt gaagaaaaac tgtttgtaaa agagcttgca aagacaggca
     3961 agtttacaga agaagaggca agaaacttta ttcgaaggat gctgcgcgag gcatcaattt
     4021 atgaatccaa gcctggtcac tataaccggg tatgaagata gccgaacttg aactagccga
     4081 cactgcaaag caattttttg ccgaacaagg aatcacagaa ctatttcccc cacaaaagga
     4141 tgcaatagat gcagggttat tggatgggca aagcgtcctt gttgcagcgc ctacggcaag
     4201 tggaaaaaca ctcattgcaa tgcttgcaat aatgaatttt ctaaaaaaca aagaaggaaa
     4261 agtcgtatat ctgacaccac taagggccct tgcatcagaa aaattctacg aattcaaaaa
     4321 actggaaaaa attagcctgg gcagaactat caaggtaaca atttctacag gtgattttga
     4381 ctcagtagac aaagaagccg aaaacgcaga cattatagtg cttacaaacg aaaaaatgga
     4441 ttcacttatg cgtcatagtg cagagtggat tgacaaaata gggctggttg tggcagacga
     4501 agtccaccta attggtgatc aggacagggg cccgaccctt gaagtacttt tgacaagact
     4561 taaagagatg gagacaaaac cgcaaattct tgccctaagt gcaaccgtga caaactctga
     4621 tgagctatca gagtggcttg gatgtgcact agtcttaagt gattggaggc cggtgcctct
     4681 aacggaggga gtcttcgatg gtggaacaat tctgtggaat aacggtgaat cagtagaaat
     4741 tgaatcaagc atcagaggtc caccagttga tctgtgtatt gacacaataa aaaatggcgg
     4801 acaatcctta atctttgcag aaacaagaac gcgttctgcc tctttggccg caaaaggggc
     4861 tgatgctgta tcaaaatttc tctctgatcc tgaaagaaaa taccttgatg agatctcaaa
     4921 aaaactactt gatagcaatg aacatacaga gttagtaaaa acactagctg cacttttaaa
     4981 aaaaggcgtg ggctttcatc atgcaggcct caaccaaaac tgcagacaaa taatcgaaac
     5041 cgaatttaga ggtggaaaaa tcaaattact tgcttcaacg ccaacgcttg ctgcaggcgt
     5101 aaacctacca gcacaacgag tagtaatatc aaacattgca agatatgatg tcaaatctgc
     5161 taccaacaag ccaatcagca ttttagaata caaacagctt tgtggccggg caggaagacc
     5221 acaatacgac agctttggcg aggccataat agttggcaat gcaaatgcag cagacctaat
     5281 cgattattat gtaaacggaa gcccagaacc aatcgaatca cagcttaccg acgacaaggc
     5341 attaagaatt cacattttga gccatatcgt atcaaacccg ggaataaaaa atgaggaaat
     5401 ctttgagttt ttttcaaaga ctctggcagg tcttcagaca agaaaaaaca cactgcgatt
     5461 ctcaattgag attgcaaaaa agtttctctt gcgcgaaaat ctcattgtac aaaaaggaga
     5521 aagatttgca tcaactgaat ttggcaaaaa ggtatcaatg ctgtatgttg atccactcac
     5581 tgcaatttac tttaggcgtg gaatagaaat ggcctcagaa aacaaaaaac acacacttgg
     5641 gtttttgcac ctcatttcaa gctgcgaaga attctttccc aaattttccc ttagaaatag
     5701 ggattatgaa accgtaaacg tcttgttgga aaaccactcc tcagaacttg ttgaacccat
     5761 ctcagaatat gactgctcaa ggagcctgct tgcactacac tcctggataa gcgaatcatc
     5821 tgaaatccag ctatcagata acctcaacat tgaatctggc gacatgcaca gactagtcga
     5881 aatggcagac tggctttcat actgtcttta tgagctggca aaactacttg gacgatctga
     5941 tctcttagaa gatcttgcaa ttataagaaa aagactccta tatggaataa aagaagaatt
     6001 agctgaccta gttcaaatca agggaattgg acgggttcga gcacgcaagc tttacggccg
     6061 tggcattaaa accatagatg atctaaaaaa aatcccagtc gacaagctgg ctgagattga
     6121 taaaattggc ccgacacttg cagataatat taaatcccaa ctgaaaaaag taaggtaatt
     6181 gcaagctgag ataatttcgg cgatagtggc ctcgcttatt gcgttctttg ttgttttggg
     6241 gttggtaccg ccgctgataa aacaactcac aaaacgcaat tgggttgtaa aagatgtcaa
     6301 ccgtattggg ggcgcgatga taccaaggcc tggtgggcca gcaattctta ctggaataat
     6361 tttatcagaa atttctcttt attttcttct tcctgcggac gagctaatca caaaggggat
     6421 tgcagccata ttggttacaa cctcggctgc ctttgtagtg ggctttgtgg atgacaggat
     6481 ggttcttggt ggctggttca aaccagtcgc ccttgccgtt gcagcaattc caataattgc
     6541 acttggtgca tatgatagca accttgagtt tcctcttttt ggggcagtca agatcccagc
     6601 actgtatctt ggggtgataa tattcatgat tccaattacc ggaaatacaa taaattctat
     6661 tgacgtgatg aatgggatag caagtggatt tatgaccatt gccgcctttt cgctggcaat
     6721 agcgctcttt atcttgcaaa actattcggt tgcaataatg actctgccgt tggcctttgc
     6781 atcacttgca ttttacaaat atcacaggtt tccaagcagg attttcccag gcgactcggg
     6841 ggcccttgtt cttggcgcaa tgtatggtgt gattgcaatc ataggtcacg ttgaagtgat
     6901 tgcagcaata gcactacttc ccgcagttat caactcgttt ctgtttttgt ctagcgtaaa
     6961 acgcatcgta gaacacagac agataaaaaa tcctacaact cacaccgatg atcacaagat
     7021 tacgactaca aaggaaacgg atgcaccaat cacacttgtt cgattaattg tagcaggcaa
     7081 gccattaacc gaaaaagaaa taggattgac aatatttcgg ttagcggtat tttcaggaat
     7141 tttggcaata attactgcat ttttgatgga ggtaaagaca tgggtcactc tctgatctat
     7201 atcttcattg cagcaatgtg ggcattgata ttgataggcg gagccgtact tgttacgctg
     7261 gtcgcacaaa tctcagttga gggatatggg gaacagcttg actttttgat tggctccata
     7321 atcaaggcag caattgcaat tatacttgtg gtgatctggg tactcgtact aacaaagatg
     7381 aaaaacaaaa tattccaaaa acagataaaa tcttaggcct gccacttttt ttgcttttta
     7441 tcgtattcct ctctggagta tcttgccttt gcaggaacgc cagctactac agtgttgtct
     7501 ggaacatcct ttgtcactac tgcccccatt gcaactacgc tatttttgcc aattgtgaca
     7561 ccagccttta tgactgcccg tgagccaatc actgccccat ctttgattgt cactccgacc
     7621 atttttccag agggaggata cggatcgttt gttagggcag cagcaggacc aatgaagaca
     7681 tttttaccaa tctttgagag tggtgggatg tacacaagac cttctatcat ggtattatcg
     7741 ccaatagtta cgttatagtc cacatgggct agtgagccaa tctttacatt gtttccaatg
     7801 atggtcttgt ctccaacata ggcaaaatgc caaatcttta cattttttcc aagtttggct
     7861 ttttttgaaa caaatttagt tatcaaaaga tatcacaggt gccatggatt tgcgtgttta
     7921 attatttttt gaggcagatc ttctttgttt ttttccaaaa agtcaatgtc ttgttttttg
     7981 tctctaagct cgtcaggcag cattatcggt atctcctcaa taattgggta gaatcggcta
     8041 cacttggaac agtataatgc cccctcaatt acaatctggt cttttgattc cgtttctatg
     8101 atttcaagtg gaaactgctt gtcaattgga catactagga tgtccatcat ttttttgttc
     8161 attttagatc taggtaaatt ggcactcctt ttttacttga caacaaggct gcctctgcaa
     8221 ttttggtgac atttactgca tcttctggcc tcacccttag atttactttt ccttcaagtg
     8281 cgtcaagaaa gcttttgatt tcaagcgtta atggctcttg tttttctttt gtgagcgttt
     8341 caacttgctg gtctttttga attttgactt cttgtgagag aaaatcgcct gaaactattc
     8401 cttcggtgca tacagcgtta aaatgtctta tgcgcgccga tgttatccag ttcgaagaaa
     8461 ttattgcaac tttgttatct ttgaatccca gcattattgt cgcaaaatct tcgtggtcat
     8521 gtctgatctt tcctgccctt gcaaaaacaa cctctggggt gtcatcaaaa agccacatcg
     8581 cagtgtctat gtcatgtaca gaagtgtcgt aaattatgcc aacgtccttt atgtggaggg
     8641 gcattctgtt ttctcgatga aactctagca ttatgagatc gccatatttt tttgatttca
     8701 caaaatcctt tactccttca actgccggat tgaatctttc aatatatcca caggtcagta
     8761 agacaccatt ttttttggca agctctaaga gccgtgtccc ttcttctgaa tcatacgtca
     8821 ttggtttttc tacaaaaacg ctcttctttt tctgaataat ctttgtcgca atctcaaaat
     8881 gagtcgccgt tggcgtgcag acaaatactg catcgacgtt ttcttctagc atggcatcaa
     8941 gtgaggtata gtagttaacg ccgtatttct gtccagtttc ttttgccctg ttttggtctg
     9001 catcacaaat tgctgacaaa acgccaagct gtgataaaac cctgacgtga tttttacccc
     9061 atcccccaac gccaatttga gcgattttca tgctagtaca ccggggttaa ccactcaaaa
     9121 tatttttcat tttttgccat tactacatcc atgtatgcac tcatcatgtt ctttgtaacc
     9181 ttgccaactt ttccagagcc aatcttttta ccatcgatgc tcataatggg tgtaatttcg
     9241 gcagcggtgc ctgtgagaaa gacttcgtct gcaagataaa tctggctgcg gctgatctct
     9301 ttttctttga cttggtatcc aagatcttct gctagtttca ttacagaatc tcgcgtgatc
     9361 ccctcaagtg cagaggattc tagaggcgga gtcagtagaa tgtcgttttt taccatgaag
     9421 atgttttctc ccggggcctc acttatgttt gcgtgcaaat caagcaagat tgcttcatcg
     9481 tatccattac gcttgcattc ctgggtggcc aaaattgaat ttagatagtt tccgcccatt
     9541 ttggcaaggg tcggagttga ggcatcacta aattttctcc acgaagaaat gccagcactt
     9601 attccatttt tgttaaacag atctccaaag ataaacatga atgcagctac gtgtgttggc
     9661 accgtctcat ttacgtgcaa gtttattccg taatctccta caaaataaaa cggacgaata
     9721 tagcaggatt tttttgcgtt gttttttttg cacaaattag ttattgcgta agctagctgc
     9781 tcgtctgaat aatttagcgc aatagagtaa aactggcctg atcttcggaa tcgcttgata
     9841 tgatcatcta gtctgaagat gtagagattt tttgaattcc aatatgctcg aattccttca
     9901 aaaatagatg ttccgtaatg aattgcatgt gttgttattg gaaccttggc tttttcaagt
     9961 gtcgtaaatt taccgtcaaa ccaaacaact tttgaaacat ttaatctcat ggaattttct
    10021 ccaaacgcgg tattaatcta tcttgtctta tggcctgctt ttggaaattt cattccagca
    10081 attcgtgttg tcgcatctgg tgcagatgcg tattttttta caacatccca actttcctcg
    10141 attattttca ctacttgttt tggaacatcc tctttccaag tgctttcttt tcccatcgct
    10201 gcgtcgtaca gcttgttttt tacagaggat gctgagacct tttttcgcgt gccaaccttt
    10261 ggattcagcc tgtacgcttt gagcatcaag ccttcagttt tgtctccagt gtatgtgtaa
    10321 aagtcttcat tgattccctc aagaatttgg cttcttaatt tccaagagta tggggatagt
    10381 attgggggga aatacttgaa aaatggagcg taaaaagtat agttagaaga gacttcaata
    10441 gaattgccaa aaacagcagt tagcattttt tttcttgtct caaaactgaa cggaaaactg
    10501 cggctgttta cctcaattcc atcatctaaa aaaaccaccg gcatgacttt gactttggca
    10561 tcttttttta actcctcaat aatctctaca tgcgcgtttg tgactgggtt taggtgtgca
    10621 aggtagattg caactgtcaa acatcttgat taaattcttg tcctatttca atgcttgaaa
    10681 attactgggg acgatgggac ttgaacccat gatctccagc gcccgaggct ggcagtatac
    10741 caagctaacc tacgtccccg aaactgaaat aaaactatgc aatattactt ttgagctata
    10801 gtgctatccg taggactcga acttgacttc aaagcttccg tctggtcgct tgtttcgctc
    10861 agtcacaaat ccctttgcgc taaggtagtc catacaacca tcgatagtgc cctgccatcc
    10921 acaaatgtaa aacattgtgt tttgaggagt aactttttca ccaattaact gttctagtgg
    10981 cgatggatca ccatcttttg ttctaaggaa ggtctctact cttccagcct gaccgctcca
    11041 cgatctgtta aaccattcct gtgggcgact tattgcggct cgatatttaa aattccactt
    11101 gtcacggcct ctatcaagac tctccatttc taaatcagta aacaagtcct tgtaactcag
    11161 ttcatcaata tagctagcac catgaagtac tacgatctct cttttatcgc ctgtggagtg
    11221 taggtgttgt gcaaagctca caaatggtgc aatacctgtt ccaccaccaa gacaaacaat
    11281 tcttctttca tctttttgac cgtctggcaa tttatcatta attagcaacg ctgcacctgt
    11341 gggttttatc caagttagtt catcgccttc ttttgcattg aaaagctgag ttgttagtct
    11401 gccaggcaat ggttttctta cccatctaat cacaagctca acatactttt tgttctcagg
    11461 gtgtgatgca atagagtatg ctcggcggat taccttgccc tcacttggaa catgcatacc
    11521 aattgttatg aattggccag ctttgtattc tggtatgact ccgtcatttg gaacaagtcg
    11581 gtaaattgac aaatcctctt ttagtaattg aatgtaagta atggtcgctt ggttttctac
    11641 taccacagac agacctatta gatgacggtt aaatatttta tgataagcta ttgccaaaat
    11701 ttgttgcttt tagctaaagt taataatcaa tctacaaact tgtaaaacga tcagaatttt
    11761 ctctctgatt atttgggccg gtcgtctaga ctggtaggat acgcccttgg catgggtgag
    11821 atcgagggtt caaatccctc ccggtccact tacaaattat ttttggcaag tactaggagc
    11881 ttttcaattt caggatcgtt ttcttttgaa agctcctcta gcttttcaag aaggtttttg
    11941 tcaccaatat cgattgctga cttgatggcg ctctttttta cttcgagatt agaatcatcc
    12001 aaacatcttc ttattgcaag gtatgcttgg cctgccttca aatgtccaag tgcgccaact
    12061 gcacaagagc gaaccatggc actttcatct gaggtaagct caacaatttt tgcaatcgcc
    12121 tttttgtcgt ttctgtttgc caagatcagt actgaataac cacgtatgtt cttgttttgg
    12181 ttttttaggc cctcaattaa aatttctgat atgtcgtttt catttaacat taatgtgctg
    12241 aacgcctcgc ctcttacctc aatgtcctta tcatcaagct ctgaaattat caggttgagt
    12301 atttttgggt ctttggcatc aatctctaat tcaagactgt cgatcttttc ttgtttagag
    12361 ccagactcta tgattttttt taattcctca aatgaggcca tttttacttg ataattacac
    12421 gtcagttaat aagaaatttt tgcaatttgc ttatagtttc cgtttttagc tttaaattat
    12481 ccatatatag ttaggagaac atgtccagcg aaaacagaga cacaatcttc attggcaaga
    12541 aacctttgat ggcgtacgtc acgtctacgc taattcaatt ggcgaacttg ccagcagtgt
    12601 atatcaaagc cagaggactc agcatcgggc gtgcagtaga tgttgcgcaa atcatctcaa
    12661 gaaaaaccga aaatgctgga tactccattg gccaaataaa gataggctct gaacaactag
    12721 aatcaaaaga cggaaaacaa cgaaacgtat ctacaatcga gatcgaagta aagagaaaca
    12781 ccccataggg gcgctttact tgaacttttt tattttattg tttgtatcgt agttctagct
    12841 ttctgaactt tgatagctca acgatttcgt tgaactcatc aaagcttacc acattctttt
    12901 ttagtttcgc agttgtacct gactttcgca gctcttttag tatttgaagt gaagcaaagg
    12961 tattggcata gagcacagaa agtgggtaca aaattatctt aaaacccatt ttgtgcaatt
    13021 ctttggcaga gatgatgggt gttgcacccc cctcgatcat gtttgcaaca agaggggcat
    13081 taatcgactt tccaattttt ttcatctcag caatagactt tggagcctca acaaagactg
    13141 catccgcacc tatctttttg tagtatttgg cgcgctcaat tgcctcatca aggccccttg
    13201 ttgcccttgc gtctgttcgt gcaactatga tgaagtcttt gccttttttt gcatctactg
    13261 cagcttgcag tttttctgca tattcttcct tttcaattac ttcttttcct gccatgtgac
    13321 cacatctttt tggccatctt tggtcttcta gaaaaattcc cgcagcacca gcagcctgaa
    13381 gctcgtttac aagcttccag acacttagtg catttccata acctgtatca gaatcaacaa
    13441 taactggaac tgagacagcc ctgcaaatcc ttcttgcatt ttccaccgtt tcagtcgaac
    13501 caataaaacc atagtctggc atcccaaaca aagtagccga tgtcccatat cctgtctgaa
    13561 acattgcctc aaagccaacc ttttgcgcaa tttttgcacc tattgcgtca tagactccgg
    13621 gaatgacaag gggttttgag ggatcgttga taagcttctt tagggttttc aacaaaaaac
    13681 atgctttgct taattattta tgattagctg caaaagattc tcggtatata caagaccaaa
    13741 ctacgtagtg ttccgtatcc tacataggat ttaattatct ccccctcaag ttcaatccaa
    13801 tgacaaagat attacttcaa ttagtaattg cagcaggtat actgtcttca gtctttgtac
    13861 tcacaactgc gtatgctgaa atttgtagca gaccatgtga agcgccaaca ataggcgtac
    13921 tagataatgg acaacgaata gtagaaaatg ggctagtcat tagcggtagg gcatttgatg
    13981 tagaaaatca gatccaaaca attccaacta tgacacttaa cacaggaacc ttggctagaa
    14041 taaagttgat ggtctatgac aatgacggcg tagatgcatt aagaactgca tctattacca
    14101 tatctgacta tcaagacgac aaaaatcgaa aggatatggc cagaatctcc tttaaccaag
    14161 ccttcacagg tgtacaaaca gtggatgtaa tggataaagg cggtcttcta aaggacgcta
    14221 gtgcaacggc aacacaactt gacgatttcc gtacagaagt ggtgttttcc ttcagaccag
    14281 taaagccatt tgatacatca gcactcattg tagatttgtg ggatcaagac caaagtgcaa
    14341 gaacaaacgt cttccttggt gctgttaaaa caacaggcaa ggaaatggtt gactttgtac
    14401 ctggaatgcc aagtcaggtt ccagcgccac ttaaacaagt agaagatgga actgcgccag
    14461 aaaacgtaga atgcagagct ggatttgagc tagtaattcg cacaacgggt gcaccagcat
    14521 gtgtatatcc atttactgca gaaatactta gaagctgggg aatggttgcc taaacggcaa
    14581 ttttcacaca atcttctttt tttaatacaa aaaattattc gattttgatc tcttttccag
    14641 ctttagattc ttgattgtct agcttttgaa gctcggattg caggaacttg cggtattttt
    14701 gcatctcttc ttttgtcatc ttagatacgt ttgagctgag gagtgtgccc atcatgttca
    14761 ttctttctat gatgtccatc acaacaaacc ttccaacgtt tttgactttc attagcacct
    14821 caagctgatt tttaattatc ttgttaattg cctcaagatc gtcagcagtg gctcgaccct
    14881 tttttgttat ttggtatttg ccatcctttg tttcctcaat tagctcctca tctaagagtc
    14941 ttccaagtag tggatagatt agacctgggg agggcttcca ttttccctca ctttgctctg
    15001 tcgctgaatc gatgatttct tttccagtgt atggctttgt tgcaagcagc tccaaaatgt
    15061 agtatcttga aaatcctcga ggaattgagc ttccaacccg ttggaaccat tggcttgtca
    15121 tcactagcga tatcactagt gataaatata aagattacgc gtgcctaatt acgttttatt
    15181 tttacgatac ctatttatcc gattttcaga agttcatggt agcaaatggt tgattctatc
    15241 gattatgatc taattatagt tggatcaggc cttgctggtc tgcgtgcagc catagaggct
    15301 gcaaagacaa gtccatcact taggattgga gtaatatcaa aagttcaggt aatgcgttct
    15361 cattctgttt ctgctgaagg cggtacagct gcagtactct atgaggaaga aggtgacaac
    15421 atcgaatctc acatatatga tacggtaaag ggtagtgatt ttctgggtga tcaagacgta
    15481 gcagaaagaa tgtgcaaaga aatgccaaaa gaaatctacc agcttgaaca ctggggaatg
    15541 ccatggtcta gacgaaagga cggacgagtc gatcaaagaa actttggtgg ttatagtttt
    15601 ccacgtgcta cttttgcatc agataaagtt ggattttttc taatgcaaac actttacgat
    15661 acatgccaaa agttcgacaa catcgagttt ctcaacgaat ggtttgtcac aagcataatt
    15721 catgatggac aacaattcat ggggataacg gcaattgagc tatcaagtgg aacattctat
    15781 gtgataaagg caaaatctct gatcattgcg tctggtggtg ccggaagact gtacagcttt
    15841 gcaacatatg cttattcgtc cactccagat ggtctcgata tggcatttcg tgcaggactt
    15901 gcattaaaag acatggagtt tgtgcagttc catcctacag gaattctacc ctctggcatt
    15961 ttgattacag aagcggcaag gggtgaaggt ggataccttt tgaacaataa cgacgagaga
    16021 ttcatgaaaa aatatgctcc aggcaagatg gagctggcac cgcgtgatat tgtatcaaga
    16081 tcaatgatga ctgaaatccg cgaagggcga ggctttaagc atgaaacagg cgttgattgc
    16141 ttaaagcttg atcttaggca cgtcggagat gccagaatca gagaaaaact aggcgcaatc
    16201 cgagaaataa gcatcaaatt ctctggcata gacccttcca aagaaccact tgacgtcagg
    16261 cctgtgtgtc actatatgat gggtggaatt cacacaaaca tcgatggcgc aacagaactc
    16321 aagggtgttt gggcctcagg agaggcagca tgcaacagcg ttcatggcgc aaacaggctt
    16381 ggagcaaact ccacttcgga atgtattgtc tggggcaaaa tcacaggcga tcttgcagca
    16441 aaacatgcac taaatgctaa atcaacttca cagtttccac atcacctagt taccttggag
    16501 gaaaaaagaa tctatgatgg aattttcaga ggacagggag aatacaatcc atacgaaata
    16561 cgacaagaac taaccgacac catgaatgac agggcgtatg tctatagaac agagactgat
    16621 ctgattgagg gattaaagaa gctgcgagaa ctaaaagaaa aaacttggaa acacgtagat
    16681 gacaaggcaa aggaatacaa cacaaacttt agtaatgtta tggagcttga ttccatgttc
    16741 agagttgcag aagttgtgct tgttggcgca atcaacagaa gagaatcacg tggagcacac
    16801 gcaagacttg attatccaca aagagacgat gcaaactttt tacaccatac ccttgcttac
    16861 tatgatccaa aggagccaat aatgaaaaaa catccggtga caattactag gtatcaacca
    16921 gtggagagga aatactagat gaaacgaaac gaaaacaaag aaggaatcaa aggaatggcc
    16981 aacccaggcc gatacggaat agaacgcgtt gcatattggc ttatgagaat aacgggcctt
    17041 ggcttgcttg cctatttgat tgggcacata tacgagacaa gctccattct aaatggaagg
    17101 gaagcatgga acgcaatgct ggatctgaca cagacaacag agggccacat cattttgaca
    17161 ctagtaattg gtatgtgtgt ctttcatacg gtaaatggaa tccgggttat gcttgggcac
    17221 gggggagtcg gagtcggcaa accagcaaga cctgattatc cttacgttcc cgcatcgcag
    17281 aataacatgc acaagctttg catttacgcc tcaattggac ttgcagctct ggcaatgatg
    17341 tatggcctca gcgtattgtt aggtgaataa aattgcgtga aagcataata atgaaaatcc
    17401 actatggcac ggcacttgga gcagtagtgc ttgtcgcagt acacatcttg ttcagactga
    17461 cgatggacta cggcgattcg ttacaatatg agaacgtgat tgccaactat gagttcattc
    17521 catatgcaat aatgctggag ctcattctga ttttgctgtc tgtccacgga ttcaacggac
    17581 tgcgagtcat actcttggaa ctaaagcagg gacgctctta tgaaaaagca gtaacatatg
    17641 gctgtcttgc agcaatggca atactaattg cctatggttc aagaactata ttgatggcaa
    17701 gcatggggat gacatagatg agtgaagtat cacaaaacat ggcagaggaa aaatcatcta
    17761 cagaggtttc atcatcaaag acaatcactt tacgcattgc aaaatacaat ccggaacacg
    17821 acgcaacgca acaatttgcg gaatttaaga tcccatatga gcgatggact acggtacttg
    17881 atgcaatctt ggatgtcaaa agacacttgg atcattccgt tgcggtgcga tattcttgca
    17941 gacaggcctc gtgcggctca tgtggaatga aaataaatgg aaggccaaga cttgcttgct
    18001 ttactaaaat ctcagagctg aactctgatg taattactgt tgaaccgatg agcaattttc
    18061 cgatagttcg tgacttggta gttgatttta aaagaatgtt tgcaaaccac aaaaaaatga
    18121 tgccttttgt aatacgagaa gattctgaag ttactggaaa tacaaaagag ttttctcaaa
    18181 gcccagaaga gctagagcaa tacatccagt ttgcaaactg tatcaagtgt ggcctttgca
    18241 attccgcatg ccccacgatg gcaacagact cgtcattttt gggcccacag gcactagcgc
    18301 aatcctaccg atatgttgca gataatcgag acaaaggaaa gaacaagcga ctaaagataa
    18361 ttgatgaatc acacggaatc tggcgatgcc actttgctgg ctcgtgcagc caagtatgtc
    18421 caaaaggagt agaccccgca atgggaattc aacttcttag aggctacatg cttggattta
    18481 gaaaataatt aggcctgctt ttttggattt gggctgttgt ttacctgatt gttgatagct
    18541 tcggccataa agtggtttga gacgtttacc ttaacgtcgt ctattccgtc aattttgaga
    18601 aggttgtcat gaacgtcttg gcaaatttta aagccaaata ctgcagggca gaaggggctt
    18661 gtcaagtgca aatctacctt tactgcagag tcattgatgt ccacattgtc gataagctca
    18721 agctcggtaa ttggggtgtt tatttcagga tcaacaattc ctgataattc atcaaaaatt
    18781 ttccgtctta attcttgtaa atcttgagtc ataaacaaca aaacatctat gctatatata
    18841 accattataa atcctagttt gatgtatcga tcccgtcttg acaaaagttt agacaaacac
    18901 acacttgatt atgtttcatc aattcacgat gatttgcaaa tcgcgcaata tgacatcatt
    18961 ggaagtcagg cccacacaat catgctttat gagaacaaaa ttctaaacaa agctgaaacg
    19021 aaaaaaattc ttttggcgct agagaagata aaaaaggaaa aatttgtcag ctccgattca
    19081 gaagacattc atgaattaat tgaatcacta gtcataaaaa aaactggact caaaactggt
    19141 ggaaaaatgc atactgcccg atcgagaaac gatcaagtgg cacttgacct taggatgaaa
    19201 atacgggacg acatcaacac aatttgcaag tgcatacttg attttattga aaccctggct
    19261 tcagttgcac aaaaacacgc agatacggca atgccacttt acacgcacct tcagcaggca
    19321 caaattggca cgttttcaca ctttgtactc tcgtatatcg atgtgttgtt tagggacatg
    19381 gagagattct atgttactta tggtagaata aacgagtcac cacttggggc aggacctgtt
    19441 ggtggaacaa gtattacaat tgatagaaac agtactgcaa aaatgcttgg cttcaaaggt
    19501 ttagtggaaa actctattga tgctacaagc agccgagatg tggttgcaga atttgtaagc
    19561 gacgtagcaa tcttgatgac aaatcttagc aggatggcag aagacatcat aatttggtca
    19621 acatcggaat tttcctttgt tgagcttgcg gacaaatttt catcaccatc cagtgttatg
    19681 ccacaaaaaa agaaccctga tcttttggag ctgactaggg gcaagactgc ccgtgttgtg
    19741 gggaatctag tagctattct gactactgtt aagggcctgg catcaggtta tgggcgagac
    19801 ttgcaggaaa tcaagccgtc tgtatggtct tcctcaagaa ttgctatctc atcccttgtg
    19861 gtattgaatg cgatgtttgc tacactgaag gtaaacaagc agaaaatgaa gaaggtggca
    19921 agctctggat atcttgttgc actagatgtg gcagaagcac tagtaaaaca aggaatacca
    19981 tttaggactg cgcacaaaat tgtaggacag ctagtgcaag gagctcacca attaggcaag
    20041 tcactatctg agctgacact tggcgagatc aaaaaatcag taaaggaaaa gggagcagat
    20101 ccaaaaaaac tgctcaaaat aatctcatca actaatacac aaacctcgct aaagggtaga
    20161 acctctcaag gatcttctgg cttttcggaa caaaaacgaa tgattgcaaa cagaaagtct
    20221 aaggctcagg cgtacaagtc aggcataaca aagagaacaa acgaggtctc aacagcattt
    20281 cgtggactct cgacaaaagt ttcgagtttg gtcaagtagc gggttcgcgg ggattcggac
    20341 cccggacagc cggattaaaa gtccggtact ctacctggct gagctacgaa ccctctcgga
    20401 attctctaaa tcgtgtataa tttagtcttt tactagcaag tttctaactc aaacagtggt
    20461 aaattaatta gattatcacg taacaaccaa ccattctttt tttgatgtag tcccaaactt
    20521 taaaaaagga acttcaatca aaaaatttac cgcccttgta gtatagtccg gtctagaatt
    20581 cggccctgtc acggctgaga cgcgagttca aatcccgcca agggcgctta caattttacc
    20641 tgatttttaa tttctgaaag aatttcttga cttgctgctg caataaacga aagccttgtc
    20701 tcatattcaa gatcagagtc aagcggcttg aggttttgat ccaagattat tcctcctgct
    20761 tcttttgcaa taagatatcc agcagccatg tctgttacgc gaattttttc tcttaaatca
    20821 acataaacat caatcatacc acgtgcaaaa agtgcaagtt ctagtgcatt tgctccaaga
    20881 tgccttgaat gattcgaatt ttcaaaaaga ggctgaattt tttttattaa ttgaagcgtt
    20941 gcaccagata cgtttattcc aacaattttg taaattggtt tttccttatg aacttggatt
    21001 ttctttttgt tgcagaacgc gccctttcct tttgttgccc aatacatatc gcctgacgac
    21061 aaatcagtta cgactccggc agttattgag ctaagcttgt tttctgttgc aaacgcaagt
    21121 gagcaacaaa agaaggggat tccacgaacg gcatttgcag agccgtctat tgcgtccatt
    21181 attacaaaac ct
//
LOCUS       DASWKH010000096        14271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2848633, whole genome shotgun sequence.
ACCESSION   DASWKH010000096 DASWKH010000000
VERSION     DASWKH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848633"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3915
                     /locus_tag="VGA92_07475"
     CDS             <1..3915
                     /locus_tag="VGA92_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9846286.1"
                     /translation="PLDFHLMENETPFSQMSQTRKSTGSEMAQIKYNYTGSGIKIAII
                     DTGVDFSNPDMQDSLARDQNNHPIMLDPDGQGLVLTNATFIANIDKYGILENVTKPVI
                     EKINKSLSKNATSYVYVTKSGVFLNLKQTGKGTQVSLYNSFFPASGPFPIFNGTIVDD
                     YKIGKTNRDYIKSASGVYHFGIIYQGAISGPFASVQAVPVLVVDSQTPGVYDTIIPDM
                     STSWKDYTRFDLKRGQTPNYDFDFTDETPIKLGDGKEFLVYDFNNDGKLDYSAGTVGA
                     QILDIYGVISQNKSAIDDILKATNGTLLPGLDPAGNFFGVMSDFVGHGTSAAASISSK
                     GTQQYDIYNNTKKYTLPGVATGAKIIPIKALWFGDTVYGWLWAAGFDNKNNTWSFSGK
                     PRADIISNSWGISNFPSLKTSPGVDILSLISNVLSVPQSVDERYPGVTIVSSAGNAGH
                     GYGTMGLPNAAPFVITVGATTNNVYVGYGAFKGQPRFGNTTTYANDMIDFSSRGPSII
                     GDPKPDLVNTGAYGFTPKNVLKSSPDSKAEPFVLFGGTSMAAPLTAGSAAILMESLKD
                     KNQDYTPFTIKNILMSTATDLQNDPYTQGAGLVNAYKAVRFVDGKDGIFIVHNDSTYQ
                     NIKEILDAPIMKLNSTLIGLDKFMLPDKTHQQTSWFAGRLVQGERSSSYFTIDNPNNH
                     TLSIQITPQHLQLIEKTELNSTTRLKLQDSQYNKTGTYRPDYIRLADIKSHESLATFY
                     ENATIPKDTSLLVLNLNFEFSDFMNKTEELYASDTKIASLYLYDWNDKNKDEKISSNE
                     LSMVNRGGSWGTVQELRVSEPQSKFKNTPLAGVYPVPTTYSYWLGETKKNATEMDYLL
                     SASYYKKDTWDDIWLDTGLVVIPPFSSAKILATITIPDDAKPGLYQGFITFTGQNHTV
                     NAPVSYAVTTPVKQKDKLLIISGTPDSDILYGNGYVKGAFDMVNRYNAGDWRQYYFDI
                     QDSTINAASIDISWKNPDTNFSIFMVDPQGRIVQTNMPPGVFGQFLDWPTSDWLGTSV
                     FSEGGGFYPVKNKDDTSTLLYTTINQTGTYTLLVHSTLFGGKSTTEPFTVLARFSTIL
                     DDDTPPQISLPIPSYTNSSAFPVPQITDESEFSVRYFLDEAEIRLDTVPDSIGEGPHS
                     LRIEATDEAENTSAQFYSFTVDKTPPNIIVNSPINNTKISDVLVVDFMVEDENLLDEQ
                     TMILFADQIIQNKTRTEINVSNFTEGTYQIIISANDRADNKIQKLVIFEIDRTLPTNI
                     VQTTAKTATDQNFVYLVIAGVAAIAIASGVVFATKSKKSPRP"
     gene            3954..4562
                     /locus_tag="VGA92_07480"
     CDS             3954..4562
                     /locus_tag="VGA92_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9846287.1"
                     /translation="MSKLDSTKANTLSRRDFLKLMGAAGTALTFAPFVPWGNFMPNPD
                     TAKLEKVPAILPDGNQANVNTFPVNHAEIVTYPQTGDRVLDEEAFRKWQFIRLPEELG
                     GGTNEISAFRVYSMVCLHLWCLWKYWPQEGRKRGECPCHGSMYNPLTGTAFAGPASLQ
                     APPSNTLANLNLEADSNGDIWILPAKWGPNDNGVVGYGRFVK"
     gene            4543..6048
                     /locus_tag="VGA92_07485"
     CDS             4543..6048
                     /locus_tag="VGA92_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX9846288.1"
                     /translation="MAVSLSRRTGAVSFFYWLWDGIDRTIFTGMKFSFPSRFVSPFGF
                     LGMLTFITFVILGISGALLMFYYFPILDRAWDSVQKINDVVPFGFHIRNIHYHGSNAM
                     VLLAILHMYYQYFSGRYKIRNEVLWVTGVILGTVTILEAFTGYDIIFSERAELAISIA
                     ASLTNSIPIAGPTIREAMFGSGFTDFVLRFYAQHVFILPLVMLGLMAVHFPRFMVFDV
                     PMVMAISGAILITGGVFPIDMGFKFEPTVPPGITVPEWYLTGLYAFLRTQYDKFVTGV
                     LWPGLFIGALLLTPFIDRYKKFSWKDRPLITAFGITGIAQIMVTTYWGFYIPPDTTIP
                     LVQRLVIDPIFFYLVMILLVPLGFGFSYMMIKMAQEAERKAKLSKEKGPKKVAEINLS
                     QKWLNWIIVALIAFQVYLNIAAYNAAITGLNNLSLFFTGLILIVFAGLFHVYRYALSE
                     AKKPPPPPPVPSYEPALEKAQPAEISSAPAKELPKEVSAQEVPKSETKSTS"
     gene            6115..6576
                     /locus_tag="VGA92_07490"
     CDS             6115..6576
                     /locus_tag="VGA92_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9846289.1"
                     /translation="MKPTSSHAYGIGIIAMIVGVSVGIGYYQLYYIPESLAKPAVSHE
                     ILEPVGKTQIKMIPGSANVDQKDNFIPKLVNIQLGIDNHVVWTNDDDTAHTVTPDHRA
                     EDSYSGEFGSSGVIKPGETYEFLFTESHEIEYHCDPHPWMKGKLVITKQRF"
     gene            complement(6573..6920)
                     /locus_tag="VGA92_07495"
     CDS             complement(6573..6920)
                     /locus_tag="VGA92_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846290.1"
                     /translation="MKDIQITLEDANIRPNSEIRGLIKVNYSGKYDSVVLNTQILNSN
                     ELMLFTSCNGKKIYQLSARLFISKDSMPENKAEFTAVIAFEPKEQHDVKFRASIIEQH
                     KEIESDVLFAKYS"
     gene            complement(6966..7040)
                     /locus_tag="VGA92_07500"
     tRNA            complement(6966..7040)
                     /locus_tag="VGA92_07500"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7003..7005),aa:Arg,seq:tct)
     gene            complement(7076..7924)
                     /locus_tag="VGA92_07505"
     CDS             complement(7076..7924)
                     /locus_tag="VGA92_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEX9846291.1"
                     /translation="MQSSLQKISVKHTIYSGQVFLWQKFDKKWYIVNGDDVLEIDEDY
                     PLQVTSYQNNDYDLFRQGDNVEKIIKTISKDPVLREAAKEFAGLRLLRQDPFQCYISF
                     IVSSNSSIQNIRLTLQRLSKKFGKKITFEGKEFRLFPTQDTLANAKESELLSCGLGYR
                     AKFVKNASIAVKEEQIDFDFLKKADYDTAKSGLLAVDGIGNKVSDCILLFSLEKLEAF
                     PLDRWIIRSLQNYYPEKFSFEGKTLTDKKYQALHEKIVDYFGPYAGYCQQFLFKLIRE
                     SNQKRW"
     gene            complement(7914..9170)
                     /locus_tag="VGA92_07510"
     CDS             complement(7914..9170)
                     /locus_tag="VGA92_07510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9846292.1"
                     /translation="MKVKVLGAGLEVGRSGFLVNCDGANYLLDYGVNLGKNLQAPLHV
                     MPKEVDSIIISHAHLDHSGYVPLMYVSGSCDVYATPPTFDLSRILIEDMLRLEKDAHP
                     FGLPEVNKMYSHAKGISYKEKIRQGNSSFELRESGHVVGGSTVLVESDGKRLFYTGDI
                     NIHGSRMLREADLDIGEIDFLITESTYSQTEQMPRQEAEKNFIEFANETLDQKGTLFV
                     PAFSVERSQEIACILKNAKFKHKIIMDGMALSVNEILLQHPDYLRDYDVFVDAIKNTK
                     WIRNQSERKKALDEPCVVISPAGMLVGGNAVIYLQQLAFDKKNGIALVSYQGEGTPGR
                     RLLDTGKVLTRGKEKKVEAEVRQFEFSGHSDRNALFDLIKKIKGNPKVLTVHGDGQSC
                     VKFAEEIHEKFGFDAHAPQLGDVIAI"
     gene            9282..9494
                     /locus_tag="VGA92_07515"
     CDS             9282..9494
                     /locus_tag="VGA92_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846293.1"
                     /translation="MGRTFDQWWNTIPADVRSKVRAGDEGNKPLLNQINWVWVKNLMD
                     KRADLNPTAAELLDWVTSGQIDAMRK"
     gene            9644..9946
                     /locus_tag="VGA92_07520"
     CDS             9644..9946
                     /locus_tag="VGA92_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9846294.1"
                     /translation="MPIAILPDVDEQRCIGCALCVEICTSLGPDVLRVKPVEGWKRGK
                     AFVFYPERCISDGACIGVCPTKSIFWMRPMNYTAGQPVPLHKNGVFVKGWAEDAGL"
     gene            complement(9964..11478)
                     /gene="tgtA"
                     /locus_tag="VGA92_07525"
     CDS             complement(9964..11478)
                     /gene="tgtA"
                     /locus_tag="VGA92_07525"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HEX9846295.1"
                     /translation="MFEILKTDLAGRIGILHTNHGKVETPAFVPVVHPVSQKIPPQKL
                     RQIGFDLVITNAYITMNRYGDEAIRKGIHGIINFDGAVMTDSGGYQVLEYGKVDVSSS
                     DMADYERKIKTDIAIPLDRPTGYGLPKKKAREYVDYTLKISKEALDGSESNGQIWCGP
                     IQGAEHSELVRKSTKSLVDYGFQMLALGSPVEVMESYEYRVLAEMIMQAKPLMPSSIP
                     LHLFGAGHPLTIPIAVALGCDTFDSASYMLYAKHDRYISEDRTSHLAEIQFFSCNCEV
                     CTKYTPKELQGLDSEQRITSLALHNLYSIKAEVDRVKQAIYEGRLWEYTIKKLRAHPK
                     LFEAIDVFTNNSKYFAKTTPKFKERALFLYGVEDQFRPELTSYHDVVRSFRSDKRKLV
                     IISDSDVKPFYLSQKYSKLKKKFDSGEVQFCQYNPFVGLIPLEISDLYPASHYVMSAR
                     TLEPDDFSEFAKTWKLFFANNRFNLVYIEDQKFLRHYAKLLPNKIKTRIIKKQK"
     gene            complement(11488..12042)
                     /locus_tag="VGA92_07530"
     CDS             complement(11488..12042)
                     /locus_tag="VGA92_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9846296.1"
                     /translation="MSIVITGNPGVGKHTIAKKLGAELGFAIIDLNQIAITKGIFEKK
                     GSTLDVDVNKLSRAMKKMIKRNTIVVGHLAPYVVPRRQVKFALVLRKSPYKLISVYKK
                     RKYSKKKAFENLGSEILGVVAYDAISRFGPSKVYQLDTTRLSAKKATSKIKSIFKKRF
                     AGDRVDWLGLVTKKGDLARFFLHK"
     gene            12116..13309
                     /locus_tag="VGA92_07535"
     CDS             12116..13309
                     /locus_tag="VGA92_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9846297.1"
                     /translation="MQIGLLGKANVGKSTFFSAATEAAVAIGNYPFTTIEPNVGVTYV
                     RTKCACKHFGIKHDNPLCVDGIRLVPVKLIDVAGLVPGAHEGKGLGNKFLDDARQAEV
                     LIHVVDVAGTTDIQGQPVPPGTHNPLEDVEFVGNEFDQWFKQILDREWQKLVREITQK
                     TATLSDGIAKRFSGLGVREFDVSGVLKESGLGTKKSSEWTDSDLLLFVKKLRKKIKPL
                     LIAANKADLCRDLRIVDEIKKSNHVVVCSAESELLLRKAAKAGLIKYLPGDSTFEILN
                     PDGLNLQQKQALSLVKTVLEKIKTTGVQEALNYAAFDLLKFITVYPVEDETKLSNKDG
                     TVLPDARLLPVGSTAKDLAATIHADLAKGFLHAVDAKTKQRISADHKLGDGDVIKIVS
                     TMSRG"
     gene            13311..>14271
                     /gene="kae1"
                     /locus_tag="VGA92_07540"
     CDS             13311..>14271
                     /gene="kae1"
                     /locus_tag="VGA92_07540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HEX9846298.1"
                     /translation="MICLGIESTAHTFSCAIMERRRKKGKILSDVRKIYHPPEGQGIH
                     PREASRHHIEHSSEVLSECIKKANLTIKDIDMISYAAGPGLGPCLRVAGVIARTLSSY
                     YKIPIYPVNHAIGHIELGKFLTGSKDPLVLLVSGGHTMLAAFKQKKWRIFGETLDITL
                     GQLLDQFGRSLGFASPCGKMIEELAEKSNNYISLPYVVKGNDVSFSGLFSAAKKISKN
                     KQDACFSLQETAFAMIGEATERALSFTQKKELMIVGGVSANKRLSQMLKKICQRQNSK
                     FFVSPLEYAGDCGSQIAWTGLLESSVKDGAKINETFVRQSWRLD"
BASE COUNT         4263 a         3124 c         2974 g         3910 t
ORIGIN      
        1 ccactagatt ttcatctaat ggaaaatgaa acgccgtttt cacaaatgag ccagacaaga
       61 aagtccacag gctctgagat ggcacaaata aaatacaact ataccggcag tggaatcaaa
      121 atagcaataa ttgacactgg agttgatttt tcaaatccag acatgcagga ttctcttgca
      181 cgagatcaaa ataatcaccc aataatgctt gatccagacg gtcaggggct ggttctcaca
      241 aatgccacat ttattgcaaa catagacaag tacggcatac tggaaaatgt cacaaaacca
      301 gtcattgaaa aaataaacaa atccctttca aaaaatgcga cctcgtatgt ttatgtgaca
      361 aaaagcggcg tctttttgaa cctaaagcaa acaggcaaag gcacccaagt ctcactttac
      421 aactcgtttt ttcctgctag cggaccattt ccaatcttta atggaaccat agttgatgac
      481 tataaaatag gaaaaacaaa ccgtgactat atcaaatccg caagcggtgt ctatcacttt
      541 ggaataatat accagggtgc aatatctggc ccctttgcaa gcgttcaggc agtccctgtt
      601 ctggtggtgg actcgcaaac accaggggtc tatgacacca taataccgga catgtcgact
      661 tcgtggaagg actatactcg ctttgatttg aaaagaggcc aaacaccaaa ctatgatttt
      721 gactttactg acgaaacgcc aataaaactt ggagatggaa aagaatttct tgtttatgat
      781 tttaacaatg atggaaagct agactatagc gcaggaacag tcggcgcaca aatccttgat
      841 atttatggcg taatttcaca aaacaagtca gcaatagatg acattctcaa agcaacaaac
      901 ggtacactcc ttccaggact agacccagct ggaaactttt ttggtgtcat gagtgatttt
      961 gtgggccatg gtacatcggc tgcagcatca atatcctcaa aaggaactca acaatacgac
     1021 atctacaaca acacaaaaaa gtacacactc ccaggcgtgg caactggcgc aaaaataatt
     1081 ccaattaagg ctctttggtt tggcgataca gtatatggat ggctgtgggc agcaggattt
     1141 gataacaaaa ataacacgtg gagcttttct ggcaagccgc gagcagacat catatctaac
     1201 agctggggca tctccaactt tccatctctt aagacatcgc caggagtaga cattttatca
     1261 ctaatatcaa acgtactgtc ggttccacag tcagttgatg agagatatcc aggtgttacc
     1321 atagtgtcta gtgcaggaaa tgcaggacac gggtatggca ccatgggcct tccaaatgcc
     1381 gcgccatttg taattacggt tggtgcaacg acaaacaatg tctacgtcgg atatggcgca
     1441 tttaagggcc agccaaggtt tggcaacacc acaacttacg caaatgatat gattgatttt
     1501 tcaagcaggg gtccaagcat aattggtgat ccaaagccag accttgtgaa tactggcgca
     1561 tacggcttta ctccaaaaaa tgttctaaag tcaagtccgg actccaaggc agagccattt
     1621 gttttgtttg gtggcacaag tatggcagca ccactgactg ctggctctgc tgcaattctc
     1681 atggaaagcc tcaaggacaa aaatcaggac tatacgccat ttacgataaa aaatattcta
     1741 atgtcgactg caacggatct tcaaaacgat ccatacacac agggagcagg actggtaaat
     1801 gcgtacaagg cagtaaggtt tgttgacgga aaagacggga tatttatcgt acacaacgat
     1861 tccacatacc aaaacatcaa agaaattctt gatgcgccaa ttatgaagtt aaattccaca
     1921 ttaattggcc ttgataaatt catgcttcct gataaaacac accagcaaac aagctggttt
     1981 gcaggaagac tcgtgcaagg tgagcgaagc tcatcatatt ttacaattga taatccaaac
     2041 aaccacaccc tttcaattca aatcacgccg caacatctac agctaattga aaaaacagag
     2101 ctcaactcta ctaccagact aaaactccaa gacagccaat acaataaaac aggaacgtac
     2161 aggccagact acatccgact tgcagacata aaaagtcacg aatcacttgc tacattttat
     2221 gaaaatgcca caattccaaa ggatacctcg cttttggtcc taaacctcaa ctttgagttt
     2281 tccgatttta tgaacaaaac agaagaactc tacgcatctg acacaaagat agcttccctg
     2341 tatctgtatg actggaatga caaaaacaag gacgaaaaaa tatccagtaa tgagctatcc
     2401 atggtgaacc ggggcggctc atggggaacc gttcaggaat tacgcgtatc agaaccacaa
     2461 tccaagttca aaaacacgcc actggctgga gtatatccag tcccgaccac atactcctac
     2521 tggcttggcg agacaaagaa aaacgcaacc gagatggatt atttgctttc tgcaagctat
     2581 tacaaaaagg acacctggga tgacatctgg cttgatactg gactagttgt gatcccacca
     2641 ttcagctcag ccaaaatact tgcaacaata acaattccag acgatgcaaa gccaggacta
     2701 taccagggat tcattacatt cacaggacaa aatcacacag tcaatgcgcc agtgtcatat
     2761 gcagtcacaa caccagtaaa acaaaaagac aagcttttga taatttctgg aacaccagac
     2821 tccgatatat tgtatggaaa tggatacgta aagggggcat ttgatatggt aaaccggtat
     2881 aatgcagggg actggcgcca atattatttt gacatccaag attccacaat caatgcggca
     2941 tcaattgaca tttcctggaa aaacccagac acaaactttt caatctttat ggttgatcca
     3001 cagggaagaa tagttcaaac aaacatgccg cctggagtct ttggacaatt tctggactgg
     3061 cccacatctg actggcttgg aacatccgtg tttagcgagg gagggggatt ttacccagtc
     3121 aaaaacaaag acgacacttc gactttattg tacactacaa taaaccagac aggaacgtac
     3181 accttactag tccattctac tttgtttgga ggaaaatcaa ctactgagcc atttactgta
     3241 ctggcaagat tctctacaat acttgatgat gacacgccgc cgcagatatc tctgccaata
     3301 ccatcataca ccaatagttc tgcatttccg gtgccacaga taacagatga gagcgagttt
     3361 tctgtcaggt attttcttga tgaggctgag atccgccttg atactgtgcc tgattcaatc
     3421 ggtgaagggc cacacagtct gagaatagag gcaacagatg aggcagaaaa cacctcagcc
     3481 caattctact cgtttacggt tgataaaacc ccgccaaaca ttattgtaaa ctcgccaata
     3541 aacaatacaa aaatctcaga tgtactggta gttgatttta tggttgagga tgaaaatcta
     3601 cttgatgagc aaaccatgat tttgtttgca gaccaaatca tacaaaacaa aactagaaca
     3661 gaaattaatg tcagcaactt tactgaagga acataccaaa tcataatctc tgcaaacgac
     3721 agggctgata acaaaatcca aaaactagtt atctttgaga ttgacagaac attgcccaca
     3781 aacattgttc agacaacagc aaaaaccgca actgatcaaa actttgtata tctggtaata
     3841 gctggcgtag ctgcgatcgc aatagcttcc ggcgttgtat ttgcaaccaa gtcaaaaaaa
     3901 tcgccaagac cttaaaaaag gtttataaga aaaatgtcga gtacggagct tgaatgtcaa
     3961 aattggatag taccaaggca aatacgctat cccgacgaga ttttcttaaa ttaatgggtg
     4021 ctgcagggac agcccttact tttgccccct ttgtaccatg gggcaatttc atgccaaacc
     4081 cggatactgc caagctagaa aaggttccgg caatcttacc tgatggaaac caggcaaacg
     4141 ttaacacttt tccagtaaac cacgcagaaa tagtcaccta tccacaaaca ggagacaggg
     4201 tcttagatga agaagcgttt cgaaagtggc agttcatcag actacctgaa gaacttggcg
     4261 gcggaacaaa tgaaatttcc gcattccggg tatacagcat ggtttgtctg catttgtggt
     4321 gtctttggaa gtactggccg caggaaggca gaaaaagggg cgagtgtcca tgccatggaa
     4381 gcatgtacaa cccacttacc ggaactgcgt ttgcagggcc ggcttcgctt caggcgccgc
     4441 catcaaatac gcttgcaaat ctaaacttgg aggctgactc taatggtgat atctggattc
     4501 tgccagcaaa gtggggaccg aacgacaatg gagtagtggg gtatggccgt ttcgttaagt
     4561 agacgcacag gagcagtcag cttcttttac tggctctggg acggaataga cagaaccatc
     4621 tttactggaa tgaagttctc attcccatcc agatttgtta gtccgtttgg gttcctagga
     4681 atgctgactt ttatcacatt tgtaattttg ggaatctccg gtgcgctctt gatgttttat
     4741 tatttcccaa tacttgatag ggcgtgggac agcgtgcaaa aaatcaacga cgtagttcca
     4801 tttggttttc acatacgcaa catccattat cacggctcaa acgcaatggt gctcttggca
     4861 atcctgcaca tgtactatca atatttttcc ggaagataca agataagaaa cgaggttctc
     4921 tgggtaacag gagtaatact aggtacagtg acaattcttg aggcatttac cgggtatgac
     4981 ataatattta gcgaacgagc cgagcttgca attagtattg cggcatcgct taccaactct
     5041 attccaattg ccgggcccac aattagggag gcaatgttcg ggtctgggtt tacggacttt
     5101 gtcctaaggt tctacgcaca acatgtcttt attttgccgc tagtgatgct tggtctcatg
     5161 gcagtccact ttccaagatt catggtattt gacgtaccga tggtcatggc aatctctggt
     5221 gccattctga tcacaggagg agtgttccca attgatatgg gcttcaagtt tgagcccacc
     5281 gtgccgcccg gcatcacagt accagagtgg tacctaaccg gcctgtacgc gtttcttagg
     5341 acgcagtacg acaagtttgt aacaggtgtg ctctggcccg gcctgttcat tggggcgttg
     5401 ctactgactc cgtttattga caggtacaaa aaattctcct ggaaggaccg cccgctaatt
     5461 acggcatttg gaataacggg aattgcccaa atcatggtga ctacatactg gggattctac
     5521 attccaccgg acactacaat tccgctagtg caaaggctgg taattgatcc gatcttcttc
     5581 tatctggtaa tgatactgct agtgccgcta ggctttggct ttagctacat gatgataaag
     5641 atggcccaag aagcggaaag aaaggccaag ctcagcaagg aaaaggggcc aaaaaaggta
     5701 gcagaaataa acctctcaca gaaatggctc aactggatta tagtggcgct aattgcattt
     5761 caggtttacc taaacattgc agcgtacaat gccgcaataa ctggacttaa caacctgtca
     5821 ttgttcttta ctggtctgat actgattgtg tttgcgggat tattccacgt atacaggtat
     5881 gcgctaagtg aagcaaagaa gcctccacct cccccaccgg ttccctcgta cgagccagca
     5941 cttgaaaagg cccagccagc agagataagt tcagcgccag caaaggagct gccaaaagag
     6001 gtatctgcac aagaagtgcc aaaatcagaa actaaatcca catcttagca aaaagatttc
     6061 agaactctga acaagcttta taagactttt ccaaaccaca acaaaacgtt gagcatcaaa
     6121 ccaacatcaa gccatgcata cggaattgga atcatagcga tgattgttgg tgtatcagtt
     6181 ggaattggtt attatcagtt atactacatt ccagagtctc tggcaaaacc cgcagtctct
     6241 cacgaaatac tagagccggt tggcaaaact caaattaaaa tgatcccggg ctctgctaat
     6301 gtggatcaaa aagataactt tattccaaaa ctagtaaaca tccagctagg tattgataat
     6361 cacgtggttt ggacaaacga tgatgatact gcacacacag taacgcccga tcatagagcg
     6421 gaagactcgt acagcggcga gtttggttct tcaggagtaa tcaagccagg agaaacatac
     6481 gagttcctct ttacagaatc acacgaaata gaataccact gcgatcccca cccgtggatg
     6541 aaaggcaagc tagtgatcac aaagcaaaga ttctaggaat attttgcaaa caagacgtca
     6601 ctttctatct ctttgtgttg ttctataatg gatgcacgaa acttgacatc gtgctgttct
     6661 tttggctcaa atgcaattac tgcagtaaac tcggctttgt tttctggcat gctgtctttg
     6721 ctaatgaaaa gacgtgcgct tagctggtag atttttttac cgttgcacga agtaaaaagc
     6781 attaattcat ttgaatttag gatttgcgtg tttagcacaa cactatcgta ctttccagaa
     6841 taatttacct tgatgaggcc ccttatttcg gaatttggcc tgatgttggc atcctccagt
     6901 gttatctgta tgtccttcac tacaagcaaa tctaattttg atgataaata atttttgaaa
     6961 ttaaacgggc cagaagggct tcgatccctt gaccactgga ttagaagtcc agcactctat
     7021 ccatgctgag ctaccggccc aagtgtttca caaatttttg gcattttaaa ggtatttacc
     7081 atcttttctg attagattct ctgatcagct tgaatagaaa ttgctgacaa tatccggcat
     7141 atgggccaaa ataatcaact attttctcat gcaaggcctg atactttttg tcagttagcg
     7201 tttttccctc aaaagaaaat ttttctggat aataattttg gagactgcgg ataatccacc
     7261 tatccagcgg aaacgcctca agcttttcaa gtgagaacaa caaaatacag tctgatacct
     7321 tgttgccaat cccatcaact gcaagaaggc ccgactttgc agtgtcatag tccgcttttt
     7381 ttaaaaaatc aaaatcaatt tgttcttctt ttactgcaat tgatgcattt tttacaaatt
     7441 ttgcccgata tccaagaccg cacgacaata attctgattc ttttgcattt gcaagcgtat
     7501 cttgggtggg aaaaagacga aactcttttc cctcaaatgt gatttttttt ccaaattttt
     7561 ttgagagtct ctgtagtgtc agccgtatgt tttggatgct cgagtttgaa gaaacaataa
     7621 acgagatgta gcactgaaag gggtcctggc gcaaaagcct caggcctgca aactctttgg
     7681 ctgcctccct tagaactggg tcttttgata tggttttgat tattttttct acattgtctc
     7741 cctgcctgaa caaatcatag tcattattct ggtatgacgt gacctgcaaa ggatagtcct
     7801 cgtctatttc cagcacatca tccccattta ctatgtacca ttttttgtca aatttctgcc
     7861 acaaaaatac ctggccgctg tatattgtat gcttgacgct gattttttgc aagctagatt
     7921 gcaattacat cgcctagctg cggggcgtgt gcatcaaaac caaacttttc atggatttct
     7981 tcggcaaact tcacacacga ctggccgtcc ccatgaacgg ttaggacttt gggatttccc
     8041 tttatttttt tgatcagatc aaagagtgcg tttctatcgg agtggccaga aaactcgaac
     8101 tggcgcacct cggcttctac ctttttttct tttccacgag tcagtacttt accagtgtct
     8161 agcagtcttc ttccaggtgt tccctcccct tggtatgaaa caagtgcgat tccgtttttc
     8221 ttatcaaaag ccagctgctg caggtaaatg actgcgtttc ccccaactag catccctgca
     8281 ggagagatta caacacacgg ctcatcaagt gctttttttc tttcagactg gtttcttatc
     8341 cattttgtat ttttaattgc gtcaacaaaa acgtcgtaat ccctaagata gtctgggtgc
     8401 tgtaataata tttcatttac gctcagagcc attccgtcca ttattatttt gtgtttgaac
     8461 ttggcgttct tcaaaatgca cgcaatctcc tgcgatcgtt ccactgagaa tgccggaaca
     8521 aacaacgttc ccttctgatc aagggtttcg tttgcaaact cgataaagtt tttctctgct
     8581 tcctgtcttg gcatctgttc agtctgagag tacgtgctct cggttatcaa aaagtcaatt
     8641 tcaccaatgt caagatccgc ttcgcgaagc atccttgatc cgtgtatgtt gatatcgcca
     8701 gtgtaaaaaa ggcgcttgcc atctgattcc actaggactg tgctgcctcc aaccacatgg
     8761 ccagactcgc gcagctcaaa tgaagagttg ccctgcctta tcttctcttt gtacgagatg
     8821 ccttttgcat ggctgtacat tttgtttact tcgggcagtc caaacggatg ggcatctttt
     8881 tccagcctga gcatgtcctc aattagaatt ctgctcaaat caaatgtagg cggcgttgca
     8941 tacacgtcac agctcccact aacatacatc agaggtacat aacccgaatg atcaaggtgt
     9001 gcatgactaa ttattatgga atcgacttct ttgggcatca catggagtgg ggcctgcaga
     9061 ttttttccca gattgacccc atagtctaaa agataatttg caccatcaca gtttaccaaa
     9121 aatcccgatc ggccaacttc taatcctgcc cccaaaactt ttactttcaa gaattaactg
     9181 ggtttgggtc catcttaata ggttattaca aaatcgccga tcattccgaa tttattgtaa
     9241 tcttcaaaag ctttaattag tcaaacctaa gatcccctca tatgggtaga acttttgacc
     9301 aatggtggaa tacaattcca gctgacgtgc gcagcaaagt tcgcgcaggc gatgagggaa
     9361 acaaaccact actaaaccag attaactggg tttgggtaaa aaatctgatg gataaacggg
     9421 ctgatctaaa tccaacagct gccgagttgc tcgattgggt taccagcggc caaatagatg
     9481 caatgcgcaa gtaagcataa ccatctagtt atctttttta tattttctaa atttttacgc
     9541 ctgactctgt atgatcaaac cgatcaaaaa attctataac gctgttgaat tttttaatca
     9601 tagtttaaat agttaccaac tatgatcaca attagtgaaa taaatgccaa tagcaatact
     9661 tccagacgta gatgaacaaa gatgcatcgg atgcgccttg tgcgtagaaa tctgtacatc
     9721 tttaggtcca gacgtacttc gtgtaaagcc agttgaaggc tggaagagag gtaaagcatt
     9781 tgtattttat ccagaaagat gcatttctga cggtgcatgc attggtgtat gcccaacaaa
     9841 gtcaatcttc tggatgagac caatgaatta cactgctgga caaccagtac ctcttcacaa
     9901 aaacggagta ttcgtaaaag gctgggcaga agacgccggt ctataagacg gcactagtct
     9961 tttttatttt tgttttttta ttattctagt cttaatttta tttggtaata gttttgcata
    10021 atgccttagg aatttttggt cttcaatgta gactaggttg aacctgttgt ttgcaaaaaa
    10081 taacttccat gtttttgcaa actcggaaaa atcatccggc tccaaagtac gggctgacat
    10141 tacgtaatgc gaggcaggat acaagtccga gatttcaagt ggaattagtc caacaaacgg
    10201 gttgtactgg caaaactgaa cctcgccgga gtcaaatttc ttttttagct ttgaatattt
    10261 ttgagagagg taaaatggct ttacatccga gtctgaaatt atcacgagct ttcttttatc
    10321 tgatctaaaa gagcggacca catcatgata tgatgtgagt tcaggcctaa actggtcttc
    10381 gactccatac agaaaaagcg ccctttcttt gaacttgggg gttgtttttg caaaatactt
    10441 ggaattattt gtaaagacgt cgattgcctc gaaaagcttg gggtgggccc tgagtttttt
    10501 aattgtatat tcccagaggc gcccttcgta gattgcctgc tttaccctgt cgacttcggc
    10561 cttgattgaa tacaaattgt gcagcgcaag acttgtgatt ctttgctcag aatctaggcc
    10621 ctgcagctcc tttggcgtgt atttggtaca aacctcgcag ttgcaggaaa aaaactgaat
    10681 ctctgcaaga tggctagtcc tgtcttcgga aatgtacctg tcgtgctttg cataaagcat
    10741 gtaggaggca gagtcaaacg tgtcgcatcc aagcgctact gcaattggta ttgtaagtgg
    10801 atggcctgcg ccaaaaagat ggagcggaat agaagacggc attagtggct ttgcctgcat
    10861 tatcatttct gctaatactc tgtactcgta tgactccatg acttcaactg ggctgccaag
    10921 tgcaagcatc tgaaatccgt aatcaactaa tgacttagtt gactttctga ccagctcaga
    10981 gtgttcagct ccctgtattg gaccacacca gatctgccca tttgattcgc tgccatctag
    11041 tgcctctttt gagatcttta gcgtgtaatc gacatattcc cgcgctttct tttttggcag
    11101 cccatagcct gtcggtctgt caagtgggat ggcaatgtct gtctttattt tccgctcata
    11161 gtctgccata tcagatgatg agacgtctac tttgccgtac tctagtacct ggtatccacc
    11221 agagtctgtc atgactgccc catcaaaatt gattatgcca tggattcctt ttcttattgc
    11281 ctcgtctccg taccggttca ttgttatgta cgcatttgta attactagat caaaaccgat
    11341 ctgccggagt ttttgtggtg ggattttttg gcttactgga tggactactg gaacaaatgc
    11401 cggcgtttcg accttaccat ggttggtgtg caaaattcca attcgtcctg caagatcggt
    11461 ttttagtatt tcaaacattt tttctcctta tttgtgcaaa aaaaatctgg caagatcgcc
    11521 tttttttgta accaacccaa gccaatctac cctgtctcct gcaaatcttt ttttgaaaat
    11581 ggatttaatt ttacttgttg ccttttttgc agaaagtcgc gtagtgtcaa gctggtagac
    11641 tttggatggg ccaaacctgc taatcgcatc atatgcaacc acgcctaaaa tctcgcttcc
    11701 aagattttca aaggccttct tttttgagta ctttcttttt ttgtaaactg aaatcaattt
    11761 gtaaggactt tttcgtaaga ctagagcaaa tttaacttgt cttcttggta ccacatatgg
    11821 cgccaggtgc cccacaacta tggtgttgcg tttaatcatt tttttcatgg ctcgtgacag
    11881 cttgtttaca tccacgtcta gtgtggagcc tttcttctca aaaattccct ttgtgattgc
    11941 aatctgattc agatcaatta tcgcaaatcc aagctcggcg ccaagttttt ttgcaatcgt
    12001 gtgctttcca acgcccgggt ttccagtaat tactattgac acacactctg ctcaaatcat
    12061 gccaatttaa tcgatctctg taatcctaat atgaagtaag aaatttcaaa ttttgatgca
    12121 gattggtctt ttgggcaaag caaatgtggg aaaatccacg ttcttctcag ctgccacaga
    12181 ggcagcagtc gcaattggga attatccatt tacaacaatt gagccaaacg tgggggtgac
    12241 ctatgttaga accaagtgcg catgcaaaca ttttggcata aaacacgaca atccattatg
    12301 tgtagacggc attcgtctgg tgccagtcaa gctaatcgat gttgcaggcc ttgttcccgg
    12361 tgcacacgaa ggcaaaggcc ttggcaacaa gtttcttgat gatgcgaggc aggcagaggt
    12421 gctgattcac gtagtagatg ttgccggcac aaccgacata caaggacagc cagtacctcc
    12481 tggaactcac aacccgctag aagacgtgga atttgttgga aacgagtttg atcagtggtt
    12541 caagcaaatt ttggacaggg aatggcaaaa gctagtaaga gaaattactc aaaaaacggc
    12601 aacactttca gacggtatag caaaaagatt cagcggcctt ggagtgcggg agtttgatgt
    12661 ctcaggagtg ctaaaagagt ccgggcttgg cacaaaaaag tcatccgaat ggacggactc
    12721 tgatcttttg ttgtttgtaa aaaaactgcg caaaaaaata aaaccgcttc tcattgcagc
    12781 aaacaaagct gatctgtgca gggatctcag aatagttgat gaaataaaaa agagcaatca
    12841 tgtggtggtg tgcagtgcgg aatccgaatt gttgctaaga aaggcagcaa aggctggact
    12901 gataaaatat ctgccaggag actctacatt tgaaatccta aatccagacg ggctgaatct
    12961 gcagcaaaaa caagccctga gtctagtcaa gacggttctt gaaaaaatca aaacaacggg
    13021 agttcaggag gcgctaaatt acgcagcatt tgatcttctc aaattcatca cagtttaccc
    13081 agtagaagat gaaaccaaac tatcaaacaa ggacggcacg gttttaccag atgcaaggct
    13141 actacctgta ggatcaactg caaaagacct tgctgccaca attcatgcag atttagcaaa
    13201 agggttcttg cacgcagtcg atgcaaaaac aaagcaaaga ataagcgcag atcacaaact
    13261 cggggatgga gacgtgataa aaatagtctc aactatgagt cgtggctaag atgatctgct
    13321 taggaataga aagtacagca cacacatttt cgtgcgcaat aatggaaagg aggagaaaaa
    13381 aaggaaagat tctctcagat gtgagaaaaa tataccaccc accagaaggc cagggaatcc
    13441 atccaaggga ggcatcaaga caccacatag aacacagctc cgaagtccta tcagaatgca
    13501 tcaaaaaggc aaacctgaca ataaaggaca tcgacatgat ttcatatgca gcaggaccag
    13561 gacttggacc gtgtcttcgc gttgcaggcg taattgcacg aacactttca tcatactata
    13621 aaattccaat ttatccggta aaccatgcaa ttgggcacat tgagcttgga aaattcttga
    13681 ctggatcaaa ggatccatta gtcttgctag tctcaggcgg ccatacaatg cttgctgcat
    13741 tcaagcaaaa aaaatggcgt atctttggtg agacacttga tattactttg ggccagctgc
    13801 ttgaccagtt tggccgctcg cttggctttg cgtcaccgtg tggaaaaatg attgaggagc
    13861 tagcagaaaa atcaaacaac tacatctcat taccatatgt agtcaaagga aacgatgtct
    13921 cattttccgg attgttttct gctgcaaaaa aaatatcaaa aaacaagcaa gatgcatgtt
    13981 tctcactgca agagactgcg tttgcaatga ttggcgaggc aacagaaagg gcgctctcat
    14041 ttacgcaaaa aaaggaacta atgatagtgg gcggagtctc tgcaaacaag agattatcac
    14101 aaatgctaaa aaaaatatgc caaaggcaaa actccaagtt ttttgtctca ccattagaat
    14161 atgcagggga ttgcggttcc cagattgcct ggacggggct tttagagtca tcagtaaagg
    14221 atggtgcaaa aatcaacgaa acatttgtca ggcagtcttg gagactggat a
//
LOCUS       DASWKH010000097         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_996841, whole genome shotgun sequence.
ACCESSION   DASWKH010000097 DASWKH010000000
VERSION     DASWKH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996841"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VGA92_07545"
     CDS             complement(<1..864)
                     /locus_tag="VGA92_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9846299.1"
                     /translation="MGKEHELTVSLEEFGLSQYEARAYVTLITKGTISASEVAYYSEL
                     PRTKVYPVLLKLQQKKLVILSKSKPVMCTGIAPEDAFDEIVHEQINKVNAMNTLVTKL
                     KKISENGKKTRGAEEKRYFHLHPNYVVNQMRVMIAGATTSIHIMADSWGISILAECKE
                     EMLSVLRRNIDVKLIIPSQSIGGESFRTIPDGIKIRMSEISQNCFIFDDTELLLIDNT
                     SGKGAVFAATDILGGNQAKLFAQVWKTGLKIDNLLEMTKSQALEICKIINIINENGLG
                     FALHSIVNSKTR"
     gene            1025..1582
                     /locus_tag="VGA92_07550"
     CDS             1025..1582
                     /locus_tag="VGA92_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846300.1"
                     /translation="MVASSVIVNDLVQVLPNPNALTITPRLEYCMYLVSKILPTLQNE
                     YERVRGSNHKSYSCNKGLTTDNPEFRKQVTAESSLACAIGALNLVENKLASVSQLSNV
                     AEILPLVSIIRTVNSTIYSVIPNESGDLVELSGILGSIIVDSGSLVEATFDFKQTNLE
                     SKQILTEVNLIVDSKINKQYPNLNF"
     gene            1599..>1859
                     /locus_tag="VGA92_07555"
     CDS             1599..>1859
                     /locus_tag="VGA92_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846301.1"
                     /translation="MKSFESTDLEKDLKRVRQFSGLVKKKILESNPNYEGKLQNLTTG
                     ELEDLDKILGMAEQILLKHQHKKEAYVLLKEFSGLLKNWTISL"
BASE COUNT          559 a          397 c          361 g          542 t
ORIGIN      
        1 cctggtcttt gaattaacaa tagaatgaag tgcaaatcca agaccgtttt cattgataat
       61 gttgatgatt ttacaaattt caagtgcctg gctttttgtc atctccaaaa gattgtctat
      121 tttgagtcca gtcttccaga cttgtgcaaa aagcttagcc tggtttccac ccaagatgtc
      181 agttgcagca aaaactgcgc ctttgccact ggtattgtca attaaaagta actctgtatc
      241 atcaaaaata aagcaatttt gagaaatttc cgacattctg atttttatgc catccggaat
      301 cgttctaaat gactcgccgc caattgattg gcttggaatg attagtttca catcaatatt
      361 tcttctcaaa acggaaagca tctcttcttt acattctgcc aaaatgctga tgccccacga
      421 gtcagccatg atgtgtattg atgtcgttgc accagcaatc ataactcgca tctgatttac
      481 tacgtagttt gggtgaaggt gaaagtatcg tttttcttca gcaccgcgtg tttttttgcc
      541 attctcgcta atctttttca gcttggtgac tagtgtattc attgcattta ctttgtttat
      601 ctgctcatgg acaatctcat caaaggcatc ttctggtgca atacctgtgc acataacggg
      661 cttacttttt gaaagaatta caagtttttt ttgctggagc ttgagcagta cggggtaaac
      721 cttggttcgc ggcagctccg agtaatacgc aacttcactt gctgaaatgg tcccctttgt
      781 gattagtgtg acatatgcac gagcctcata ctgagaaagg ccaaactcct ccaaacttac
      841 agttaattca tgctcttttc ccataggcag tcttttggtg ttattaggta agatccattg
      901 tcaaattcat tgaacaaaac ctagaaaaaa aaatccagtt tgtaaccacg gggtcttgtt
      961 actgcggtta aaccaaattt tgtgacagcg ttctaataaa ctaaaaccca atctgtgatc
     1021 aggtatggtc gcaagctcag ttattgtaaa tgatctagtt caggtcttgc caaatccgaa
     1081 cgccctgacg ataacgccaa ggctggaata ctgcatgtac cttgtcagca aaatcttgcc
     1141 aacacttcaa aatgaatacg aaagggtgcg aggctcaaac cacaagtctt attcttgcaa
     1201 caaggggctg acaacggata atcctgaatt tagaaaacag gttacggcag agtcaagcct
     1261 tgcatgcgca ataggggcac taaatctagt cgaaaacaaa cttgcatccg tttcgcagct
     1321 tagcaatgtt gctgaaattt tacctctagt atccattatt cgcacggtaa actctactat
     1381 ctattctgtc attcctaatg aaagcgggga tcttgtagaa ctatcaggaa ttctgggcag
     1441 catcatcgtg gattcgggga gtttggttga ggccacattc gacttcaagc aaacaaacct
     1501 cgaatccaaa caaatcctaa ctgaagtaaa tttgatagtg gactctaaaa taaacaagca
     1561 gtaccctaat ctaaatttct agcaagggcg ttttgctaat gaagtccttt gagtccactg
     1621 acctagaaaa ggatctaaag cgggtacgcc agttttcggg actagtaaaa aagaagattt
     1681 tagaatcaaa cccaaactac gagggaaaac tccaaaacct gacaacagga gagctagaag
     1741 acttggacaa aatacttggc atggccgaac aaatcctcct aaaacatcag cacaaaaagg
     1801 aagcatatgt tcttctaaaa gagttttctg gcctgctcaa aaactggacc atctcgctt
//
LOCUS       DASWKH010000098         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_250972, whole genome shotgun sequence.
ACCESSION   DASWKH010000098 DASWKH010000000
VERSION     DASWKH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250972"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGA92_07560"
     CDS             complement(<1..566)
                     /locus_tag="VGA92_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9846302.1"
                     /translation="MSLLSSSSESFHALITKIPVSDDKKKMSVQKKQRTSLIHNEANN
                     SMGVGLSASDDIEFEKTHMPYYDELSSDLASILGLDDLETKIYLNLLRTGPITASALS
                     KEINVDRAKTYRTIDKLSSAGIVSISFSSPKLCIAADPDEILSLTLKKKEEEIIKIKK
                     DGKKIIGKIKEVSTSESKSSVPTFRIIQG"
     gene            complement(580..1488)
                     /locus_tag="VGA92_07565"
     CDS             complement(580..1488)
                     /locus_tag="VGA92_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9846303.1"
                     /translation="MTKTKTLQKDTDAKLPKIPLVRTEKFRVMIISAVASAFVIIFSF
                     YLSGSISLITRDVGIIFGILAGVIPLTLLQLKEVQRKESVDRHLPLFLLSLVSSIQSG
                     SNLIHAIQLVPERNMGHLGPLLKNLKANISWGLSIEEGFTNFAKKAGTRMAKRVTVLL
                     EIAYKNGGNVSENLETIQKHVTELRNLEKERKSALQPYTYTIYIAYAVFIAITVILSS
                     QFFTQIESVKQLLLEQSDLPANNVFAALSGIKVTEIDSIMFNMAIIESVFGGLAAGKI
                     GSGSYVSGIKHVIIMIVIAVIAFTLI"
     gene            complement(1491..2408)
                     /locus_tag="VGA92_07570"
     CDS             complement(1491..2408)
                     /locus_tag="VGA92_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9846304.1"
                     /translation="MGLLKSKTLDEKKHRPSGFQVFSYRLLNRHLHFLYPRMPTLREN
                     IRQAMMPVPFEVFVASMVLSSMIAAIVGLISMIAASIVLNIEPIATIVLSLVASIGCA
                     GITFGALQLSPVINAKSRATKLSEEIPHYIGYMATLCASGLSLEGIFKSISKENSNEE
                     VVKDAKYVTRNIEVLGMDVITAVNDLIRRTPTGPYSELLDGAIITFKSGGNLREYFLA
                     TAKVHLEEKKLNVKRSTESLGIIAEIYTILLIVFPLMAIIMLSIMAIMNPDLGGFDLL
                     TLMNMLTYVLVPLFGVVMLIMMNSMVPKR"
     gene            complement(2408..>3360)
                     /locus_tag="VGA92_07575"
     CDS             complement(2408..>3360)
                     /locus_tag="VGA92_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HEX9846305.1"
                     /translation="SLPDGSRINLTLGHEITKRGSTFTIRRFRADPITIIDLIKFRTI
                     SVYEAAYLWYAVENNSTMLVAGGTASGKTTLLNSLSSFIRPGQKLVSIEDTHELNLLH
                     ENWIPAVSRQNFTDGQIGEITQYDLLRAALRQRPDILIVGETRGREAYTLFQAMATGH
                     GGYSSIHADSVEATLTRLVSSPMDVPKALIANTLDIITLQLKMRFNDKSVRRIIQISE
                     IAGLDDKTGEIKTHEIFKWNPNDDRHEFSGDSIVMNKIKERTGVSDDQITYELKKRKT
                     ALEWLTENNIRGHKEVMKNILEFYSNSDRFYERKRLVIES"
BASE COUNT          829 a          739 c          715 g         1077 t
ORIGIN      
        1 ccttggatta tgcgaaaggt tgggacgctg gatttggact cacttgtgct tacctctttt
       61 atttttccta tgatcttttt tccgtccttt ttgattttga ttatttcttc ttcttttttc
      121 tttagcgtca gcgacaatat ctcgtctggg tctgcggcaa tgcaaagctt tggcgatgaa
      181 aaagaaatcg agacaattcc ggcacttgat aatttgtcaa ttgtacgata tgtctttgcg
      241 cggtccacgt tgatctcctt tgataatgcg cttgcagtga tcggcccggt tcgcagcaaa
      301 ttcagataaa tttttgtctc aagatcgtct agtcccaaaa tcgaagcaag atctgatgat
      361 agctcatcgt agtatggcat gtgtgtcttt tcaaactcta tatcatcaga tgcgcttagt
      421 cctacaccca tagaattgtt ggcttcattg tgtattaacg atgttctctg ttttttttga
      481 acagacattt tcttcttatc gtcgcttaca ggtatcttcg taattaaggc gtggaaggat
      541 tctgacgatg aacttaaaag agacaaaaca gctttccacc taaattaaag tgaatgcaat
      601 cactgcaatc actatcatga ttatgacgtg tttgattcca gagacatacg atccacttcc
      661 gatcttgcca gctgcaagac cgccaaagac tgattctatt attgccatgt tgaacatgat
      721 cgagtcaatc tcagtgacct ttatgccgga aagcgccgca aagacgttgt tagctggcag
      781 atcggattgc tcaagtaata attgctttac tgactcgatc tgcgtaaaga attgagacga
      841 cagaatcaca gtaatggcaa tgaagactgc gtatgcgatg tagatggtgt aggtgtatgg
      901 ctgcagtgct gacttgcgtt ctttttccag attcctcagc tccgttacat gtttttgtat
      961 ggtttctaga ttttctgaga cgtttcctcc gtttttatac gctatttcaa gtaaaactgt
     1021 gactcttttt gccattcgtg ttccggcctt ttttgcaaaa tttgtaaatc cctcctcaat
     1081 tgagaggccc cagcttatgt ttgccttgag attttttagc agagggccaa gatgtcccat
     1141 gtttcgctca ggaacaagct ggattgcatg gataagattt gagccgctct gaattgagct
     1201 gaccagtgag agcaaaaaca acggcaggtg cctatcaacg ctttcttttc gctgcacctc
     1261 ctttagctgt aggagggtaa gcggtatgac ccctgccagt atgccaaaaa taattccgac
     1321 atcccgcgta atgagactaa tcgagcctga caaataaaag ctgaaaataa ttacaaacgc
     1381 actagctact gcagaaatga tcatcactcg gaatttttca gtacgaacta gcggtatctt
     1441 tgggagcttt gcgtcggtat ctttttgcaa cgtttttgtc tttgtcatgt ttacctcttt
     1501 ggaaccatcg agttcatcat gattagcatg actacgccaa aaagcggcac aaggacatat
     1561 gtcagcatgt tcattagggt tagcaggtca aagccgccaa ggtctgggtt cattattgcc
     1621 ataattgata gcatgattat tgccatgagg ggaaacacga ttaacaaaat agtgtaaatt
     1681 tctgctatta ttccaaggga ttcagttgat cttttgacgt tgagcttttt ttcctcaagg
     1741 tggacctttg ctgttgccaa aaaatactcc cttaggtttc cgcctgactt gaaggtgatt
     1801 attgcgccat caagtaactc agaatatggc ccagtcggag tcctcctgat tagatcgttt
     1861 acggcagtaa tgacatccat tcccaaaacc tcgatgttgc gcgtcacata ttttgcgtcc
     1921 tttacaacct cctcgtttga attttctttg gaaatggact tgaatattcc ctcaagcgac
     1981 agaccacttg cacaaagggt cgccatgtat ccgatgtagt gcggaatctc ctcactcagc
     2041 tttgtagccc ttgactttgc attaattacg gggcttagct gcaaggcacc aaacgtaatt
     2101 ccggcacaac cgatgcttgc aactaggctc aaaactattg tcgcaatcgg ttctatgttc
     2161 aaaacgattg atgcggcaat catgctgatg agcccaacga ttgcagcaat catgcttgat
     2221 aggaccatgc ttgcgacaaa aacctcaaag ggaactggca tcatggcctg cctgatgttt
     2281 tctcttagtg ttggcattct tggatataga aaatgtaggt gccgatttag aagcctatag
     2341 ctaaatactt gaaatccaga cggcctgtgt tttttctcgt ccagagtttt tgattttaaa
     2401 agacccatca actttcaatc acaagtctct tcctttcata gaacctgtcg gagtttgagt
     2461 aaaactccaa gatgttcttc attacctctt tgtgacccct gatgttattt tctgtaagcc
     2521 attcaagggc agtctttcgc ttctttaact cgtatgtaat ttggtcatca ctaacaccag
     2581 ttctctcttt aatcttgttc atgacaatgc tgtctccaga aaattcatgt cgatcatcgt
     2641 ttgggttcca cttgaatatc tcatgggttt ttatctcacc tgtcttatca tcgagtcctg
     2701 caatctctga gatctggatg attcttctaa ccgacttgtc gttaaagcgc atctttagct
     2761 gaagcgttat gatgtctagc gtgtttgcaa tcaaagcctt tggcacatcc atcggagatg
     2821 acacaagtct cgtcagtgtt gcctctacgg agtctgcgtg tattgatgag taacctccgt
     2881 ggcctgttgc cattgcctga aagagcgtat atgcttctct gccccttgtc tctccgacaa
     2941 ttagtatgtc tggcctttgt ctgagtgctg ctctgagcaa gtcgtactgt gttatttcgc
     3001 ctatctggcc gtcggtaaag ttttggcgcg agacagcagg aatccagttc tcatgcagca
     3061 agttgagctc gtgcgtgtcc tcaatgctta cgagtttttg acctggtcga ataaacgatg
     3121 ataacgagtt caaaagtgtg gttttcccac ttgctgtgcc tccagctact agcatagtag
     3181 agttgttttc tactgcatac caaagatatg cggcctcgta cactgaaatg gttcgaaact
     3241 tgatcaggtc aattattgta attgggtctg ccctgaacct acgaatggta aaggtactgc
     3301 cgcgctttgt tatctcgtgt cccaaggtga ggtttattct gctaccgtct ggaagggatg
//
LOCUS       DASWKH010000099         9230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1004753, whole genome shotgun sequence.
ACCESSION   DASWKH010000099 DASWKH010000000
VERSION     DASWKH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004753"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1463
                     /gene="carA"
                     /locus_tag="VGA92_07580"
     CDS             285..1463
                     /gene="carA"
                     /locus_tag="VGA92_07580"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX9846306.1"
                     /translation="MHFSRVTISIKKAQYANKHGKLILSDGTIFDGKGFGYSKTVFGE
                     VVFNTGMVGYNETLTDPSYAGQILTLTYPLIGNYGVPDPQAKDESGISTYFESDKIQV
                     RGLVVHELSELASHWNLKLTLDEWMYNEKVPGISGVDTRHLTKKLRTSGVMMAALAVS
                     DSEINVEQVKEQLAKAKPYTSEEFMGDVSTGQAQSIGAQKECVVVIDTGVKNAILRNI
                     RDLGYQVIRLPWNATIDQVLSYKPKGVVISNGPGDPEKCPQTIQTAKQLIEQNIPTLG
                     ICLGAQIIGLAGGAKTYKLKYGHRGQNKSCLNLQNNQVYVTSQNHGYGIDPDSIKHTD
                     FDLWFTNTDDKTVEGIRHKKKKIIAVQFHPEASPGPYDCKYVFEELDDMMKGGDSNSK
                     "
     gene            1453..4713
                     /gene="carB"
                     /locus_tag="VGA92_07585"
                     /pseudo
     CDS             1453..4713
                     /gene="carB"
                     /locus_tag="VGA92_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
     gene            4858..5484
                     /locus_tag="VGA92_07590"
     CDS             4858..5484
                     /locus_tag="VGA92_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846307.1"
                     /translation="MSDEKPKTRSKKKDGLTASEAAELAEAAAKAATAKAAAAKAAAD
                     EEAQKQYEEAAKVAAELAKAAAAGGKAFTNKRHQERIFRREMGEPEFFRYKKELVPTR
                     RVLTEDEQEEISRQVEIPTDQTPHYKPEYILSPEFGGTSNYESGVSELLVETKQRLDR
                     LKNDPNAAAADVARAEQDLKYLDSLYQNFYFGMNVFRTAKGGRAKLKE"
     gene            5499..6764
                     /gene="hemA"
                     /locus_tag="VGA92_07595"
     CDS             5499..6764
                     /gene="hemA"
                     /locus_tag="VGA92_07595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX9846308.1"
                     /translation="MDKNNFNITNVRVTFKNIPIHKIEKFAFKDIPAACEAFKKIPGV
                     SECVIIQTTSRVEIFTVAVMEKEAPDVRRGEGKNLVMHKIKETWISLTELEQFDTDHF
                     DQILEVYENTDVYLHLLRLASGLDSIVIGNETVLEELKTSISNAKQAKVSGKLLNKLF
                     ESSIRIATRIRDSTGISKGATSVGDIAVKIAQEIAGLDGKKHVLLMGTGETAAMVAKT
                     LNKNGYTFDVTSKTIERATGFSKIQGGKPIEFQDVLKGFDKFDIVFVATTADYFIINY
                     DKLKVVMEGKKSGTMILDISDPRAVDESVSTLPGVKLMFRDQITEMEERNLKAREDIV
                     LAVEKMISKEVPIMAATMQQLEPEPLVKDAFASVDSIRKKELEKALEMLGETDEKKIK
                     IIEELTKAVVESIVSVPTSSSKKASEDKS"
     gene            6922..7086
                     /locus_tag="VGA92_07600"
     CDS             6922..7086
                     /locus_tag="VGA92_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX9846309.1"
                     /translation="MPKFVCKDYGFECDFVAEGDDIAKVIAEYSKHSTDEHGIEYSKE
                     ALMQFIIRKG"
     gene            complement(7181..8026)
                     /locus_tag="VGA92_07605"
     CDS             complement(7181..8026)
                     /locus_tag="VGA92_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9846310.1"
                     /translation="MVVENKATISYVQLLKEDLVILRMVPNDGTIPEYEAGQFVTIGI
                     PVVAENYKTVRRAYSIASHPENKKYFEFVLRWVRKPLPGRVTTQLFYASEGDQITMGK
                     PTGNALTINERLPDGRKDERRIVCIGGGTGIAPFVSFAQHLRAIGDKREIVVLHGASY
                     VDELSYKGLFTKLEEESVDRGRDKWNFRYRASISRPQEWFNRSWKGQTGRVESFLKPR
                     DGGKSPLEELVGEKITKENTSFYICGWQGTIDGVMDFLGPQGFVTLHDKREDGSFEVK
                     YESYG"
     gene            complement(8190..8939)
                     /locus_tag="VGA92_07610"
     CDS             complement(8190..8939)
                     /locus_tag="VGA92_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9846311.1"
                     /translation="MATTKIVPSKPALILEGGKKHLVITDLHIGFEGNLAQNKIFVGK
                     NTTLNEFLDEVYSLVDSNKPDDIILLGDVKSGISRISKNEWDEIPKFLQELKKKTDII
                     IVPGNHDADIQRLVPDGITLISAIGVVLENVLLTHGHTIPSENFAHVDKIIMGHVHPV
                     FFDEDSVLNGQRVWVSIKTKKENIFPSSAGELEIIIIPSFNRYFYATKKKQYKRSISP
                     IIERIKDVSSARIVTLDGVIIGNEAVLSQVI"
     gene            8974..>9230
                     /locus_tag="VGA92_07615"
     CDS             8974..>9230
                     /locus_tag="VGA92_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846312.1"
                     /translation="MEKVKKTDVIISEKRVKLHIFEPSKRQIWTVVGMGGEHWLTPEF
                     DFCSCRGYYFGKFKKKKSCYHLESVKMAKKENDIEIVTFSD"
BASE COUNT         3046 a         1851 c         1976 g         2357 t
ORIGIN      
        1 acaacatcat acctaccgtc atacattgtc cagcaaaatg acattttgaa cgttgaacta
       61 cagttaatca tcaccaaaga gcacatcaaa ggcctcactt agcttgaaat gaattaggaa
      121 tgaatttgat cgcaggctaa attgcaaaaa ttaaggtcat tttaaaatgc cattaaaaca
      181 gaaaaaatga gaatagagct gtcttgtgtc atcagaaact ataactatgg ccctatagat
      241 tttttcgcga tcacaccact caaaaaactt aaatcaccaa ctaaattcac ttttcgaggg
      301 tcacaatttc gataaaaaaa gcccaatacg caaacaaaca tggaaaacta attttatcag
      361 acggtaccat ttttgacgga aaaggattcg ggtattcaaa gactgtcttt ggagaggtag
      421 tattcaacac aggaatggtt ggctataatg agaccctaac tgatccgtca tatgcaggtc
      481 aaatccttac tctgacgtat ccccttattg gcaactatgg cgtgcctgat ccgcaggcaa
      541 aagacgaatc gggcatatcg acatactttg agtccgataa aattcaggtg cgaggcctag
      601 tagtacatga attgtcggag cttgcaagtc actggaacct aaagctgact ttggatgaat
      661 ggatgtataa cgaaaaggtt ccgggaattt ctggagttga cacacgccat cttacaaaaa
      721 aacttcgaac aagcggcgtg atgatggcgg cacttgcagt ctctgattct gaaataaatg
      781 tggaacaagt aaaagaacag ctggcaaaag caaagcccta cacatcagaa gaatttatgg
      841 gtgatgtctc gacaggacaa gcccaaagca ttggtgcaca aaaagaatgc gtcgtagtaa
      901 ttgacactgg cgtcaaaaac gcaatcttgc gaaacatccg agacctaggc taccaagtta
      961 tcaggcttcc atggaatgcg acaattgatc aggtgctatc atacaaacca aaaggagttg
     1021 tgataagtaa tggacctgga gatccagaaa aatgcccgca gacaattcag actgcaaagc
     1081 aactaatcga gcaaaacatc ccgacgcttg ggatatgtct tggggcacaa atcattgggc
     1141 ttgctggcgg tgcgaaaact tacaagctaa agtatggtca tcgcgggcaa aacaagtctt
     1201 gccttaattt gcaaaacaac caagtttatg tgacaagcca aaaccacgga tacggcattg
     1261 acccagactc gattaaacac acggactttg atttatggtt tacaaacaca gacgataaga
     1321 cagtcgaagg aataaggcac aaaaagaaaa agatcatcgc agtacagttt catccggaag
     1381 catcgcctgg tccttatgat tgcaagtacg tcttcgaaga gctcgacgat atgatgaagg
     1441 ggggagattc taattcgaaa tgaaagtctg aaaaaaattc tagtattggg cagtggggca
     1501 ataaagatcg gagaagcagg cgaaagtcgc aaaaacgacc gctaattcga ctactccggc
     1561 agtcagtgcc tcaaggcaat acgagaagat ggtctgaaaa gcgtactcat caaccctaac
     1621 attgcaacga ttcagactga cacaaggttt gcagacaagg tgtatctgct gcctgttacg
     1681 ccagagtatg tccaatcaat tatcgagtcg gagcgaccag atagtataat gcttggatat
     1741 ggcgggcaga ctgcactaaa ctgcggagta aatcttgcag agcttggggt tttggaaaaa
     1801 tacggaatca aagttcttgg cacacaaatt gcaggaattc aaaaaaccga ggatcggcag
     1861 ctcttcaagg attcaatggc agagtgcggc gtaccaattc taaaaagcaa aacagtcaat
     1921 acatttgagc aggcaaaaaa agcagcagaa gaccttggct atccagtaat aattcgcgtc
     1981 gcatacacac ttgggggcaa ggggggagga gtggcctaca acgagtacga gctgcacgaa
     2041 attgtcgagc ggggattaaa cgcaagcctt gtgcaccaag tactaatcga ggagtacatc
     2101 gggcactgga agcaaataga atatgaggtg atgcaggact acgagggaaa caacgtcatt
     2161 gtgtgcaaca tggaaaacgt tctttccatg cgggttcata cgggcgataa cattgtcgtt
     2221 gcaccgtctc agacaatcga caatagggaa tatcacatgc tgcgttctgc agcgctacgg
     2281 gcaacaaaac atgttggaat tgttggagaa tgcaacatcc agtttgcact ggagccggaa
     2341 tctgataggt atgtggcaat tgaaattaat ccaaggcttt caaggtcatc ggcgcttgct
     2401 agcaaggcaa ccggataccc gcttgcgtac atgtctgcaa aaattggtct tggccactcg
     2461 cttccagagc ttgtaaacca tattacaaaa actaccactg cgtgctttga gccatccttg
     2521 gactatattg tctgcaagca cccacgctgg gattttgcca agtttgagct tgcaaaccgc
     2581 aagcttggcc caacaatgaa gtcagtaggt gaggtaatgg caattggaag gacctttgag
     2641 gaatcactcc aaaaagcaat acggatgctg gatgttggca acgacggctt ggttcttaat
     2701 cgagcaaacg gaaaatctta cgacgtagaa gacattgaaa accatctcct aaaccctgat
     2761 gataatatct tgtactatgt tgcagccgca ctcaaaaaag gactagacat acaaaaaatc
     2821 caaaagcttt ctgcaattga tccatggttc attgaaaaaa ttaagaacat tattgataca
     2881 gaacaggagc taatagaaaa gcaactggat gccgaacttt tacgaaaggt aaagcatttt
     2941 gggttttccg acaggcaaat tgcgcgagca aaacaaaaag aagaaaccga aatcaggaaa
     3001 ataagaaaag atctcggcgt cacaccggta gtaaagcaaa tcgataccct ggcagcagaa
     3061 tggcctgcaa agacaaacta tctgtatgtg acatatggcg gaaccgaaaa tgacgtctcg
     3121 gtaagctccg accagcgcgg cattatagtg cttggggcag ggccataccg cattggaagc
     3181 tcagtcgagt ttgactgggg gacagtaaac atggtgtggg gtctgcagga aaatggagaa
     3241 aaaaacgtag ttgttgtaaa ctgcaaccca gaaacagtct cgactgatta tgatatttgt
     3301 agcaggctgt attttgagga gctgacactt gagagaatat tggacattgc ggaatttgaa
     3361 aacgcaaaag gaattgtaac atctgttgga ggacagacgg caaacaacct tacgccaaag
     3421 cttgcaaaag caggaatgaa acttttgggc acatcagctg aagacgttga cagggcagaa
     3481 gaccgctcaa aattcagcag cgtcttagac aagctgcaca taaagcagcc ttcttggcaa
     3541 gcctttacaa actcattgga ggccaagtcc tttgccttaa aggtaggcta tcctgttttg
     3601 gtgaggcctt cgtatgttct aagtggtgcc gcaatgaagg ttgtctggtc gcaagaagag
     3661 cttgcaaaat atgtgacaga ggcaacgatc ctttcaccag accatccggt agtcattaca
     3721 aaattcatgc taaatgcact tgaggtggac gtggatgggg tctctgatgg aaagaacgtc
     3781 ataattggcg caatagtgga gcacatagat gcagcaggtg tccattccgg cgacgcaatg
     3841 atggtgattc caccatggcg cctctcaaac aagcacgtcg acacaataac agactatgca
     3901 aacaagatag caaaatcatt taacatcaaa ggcccgttca atttgcagtt tctaatccac
     3961 aacgaccatg tctatgtaat tgagctaaac attcgcgcgt cacgttccat gccttttgta
     4021 tcaaagctga tcaagacaaa cctcatcagc ttggcagcac gtgccgttct tggaaaagag
     4081 cttccaaaga ttccgcaaga caaatggaaa aagacacaca ttcatggaat aaaagtgccg
     4141 cagttttcgt ttatgcagct tgatggtgca gacattgtac ttggagtaga aatgcagtca
     4201 actggcgagg ctgcgtgttt tggggacagc ttttacgatg cactatcaaa gagcctaatt
     4261 tctgtcgggt ataaccttcc aaaagaaggt actgcacttg ttacgattgg cggtaccgaa
     4321 aacagggaga agcttttgca gacaatggcc acactacaac atcttggatt caaaatcctg
     4381 gcaacggagc atactgcaga attcctatca gaaaaaaaca ttgcagatgt acaaatagtt
     4441 cacaagattt cggaaccaca aagaaaacca aacatctcgg atttgctgta tgaacgaaag
     4501 attaacttta tcattaacat tccttccact tcgactctgg aaaaatatgt gggtatgctc
     4561 tatgatgaat accagattcg aagaaaagca accgagcttg gaattccggt ccttacaaca
     4621 acggagcttg ctgaatcctt tgtcaagacg ctggaatggc tacaccacaa cgagactacc
     4681 aaaaaatcac ttgagccata cgacccaatc taattacaag tatgagacaa gaaaaactca
     4741 caacccttta aggttggtta tgtgtcagct gtaattggta ttatcatttt taggatctga
     4801 ttaaagcaaa aacatatcat aaaaacttaa aatttaaccc caaatgatgt atctacaatg
     4861 tctgacgaaa agccaaagac tcgatccaaa aaaaaggatg gattaacggc atcagaagca
     4921 gcagaattgg cagaagcggc cgcaaaggca gccacagcaa aggcagcagc cgcaaaggca
     4981 gccgcagacg aagaagccca aaagcaatat gaggaggcag caaaagtggc agcagaatta
     5041 gcaaaagcag ctgcggctgg agggaaagcg tttactaata aacgccatca agagcgaata
     5101 tttagacgag aaatggggga gccggaattt ttccgttata aaaaagaact agtcccaaca
     5161 agacgtgtac ttacagaaga cgagcaagaa gaaatttcac gacaagtaga aattccaaca
     5221 gatcaaactc cgcattacaa gccagagtat atactgagtc ctgaatttgg cgggacatct
     5281 aattacgaat ctggagtaag tgaactttta gtagaaacta aacaaagact tgatcgacta
     5341 aaaaacgatc ccaatgcagc cgcagctgat gtggcgcgcg ccgaacaaga tctcaagtat
     5401 ttggattcat tatatcaaaa tttttacttt ggcatgaatg ttttccgtac tgcaaaaggt
     5461 ggaagggcaa aacttaagga ataattggtg acattattat ggataaaaat aattttaaca
     5521 tcactaacgt ccgagttact ttcaaaaaca tcccaattca taagatagaa aaatttgcat
     5581 tcaaagacat cccagccgca tgtgaggctt ttaagaaaat tccaggtgtt tcagaatgtg
     5641 tgatcattca gactactagc agagttgaaa tattcacggt ggctgttatg gaaaaagaag
     5701 cgccagatgt gaggcgtggt gaaggaaaga acctcgttat gcacaaaatc aaagaaactt
     5761 ggatatcact aacagaacta gagcaatttg acactgacca ctttgatcaa attcttgagg
     5821 tgtatgagaa tactgatgtt tatctccatc tgttaagact tgcatctggt ttggattcga
     5881 tagtcattgg aaatgaaaca gtccttgagg aactaaaaac atcaatttca aatgcaaaac
     5941 aagctaaagt atctggcaaa ctattgaata aattatttga gagttctata cgaattgcta
     6001 ctcgtattag agattctact ggaattagta aaggagcaac atcagttggc gacatagctg
     6061 ttaaaattgc acaagaaatt gcaggtttgg atggaaaaaa acacgttctg cttatgggca
     6121 caggcgaaac ggcagctatg gttgctaaga ctctgaataa aaatggctac acctttgatg
     6181 ttaccagcaa gacaattgaa cgcgccaccg gcttttctaa aatacagggc ggcaagccaa
     6241 tcgagtttca agatgtttta aaagggtttg ataaatttga catagttttt gttgctacaa
     6301 cagcagacta cttcataatt aattatgaca aactaaaagt ggtcatggaa ggtaaaaaga
     6361 gtggaacgat gatattagat atatcagatc caagagcagt tgatgaaagt gtctcaacac
     6421 ttcccggtgt caaattaatg tttagagatc aaattactga gatggaagaa agaaatctga
     6481 aagccaggga ggacatcgtt ctggctgtgg aaaaaatgat tagtaaagaa gttccaatta
     6541 tggcagcaac aatgcaacaa cttgagccgg aaccattagt taaggatgcg tttgccagcg
     6601 tagactcgat aagaaaaaaa gaattagaga aagccttaga aatgctaggg gaaacggatg
     6661 agaaaaaaat caaaatcatc gaggaattga caaaggcggt tgtggaaagt attgtttctg
     6721 tacctactag tagttcaaag aaagcatcag aagacaaatc ataattgctt gaaactgtaa
     6781 tattttagtg ggactttgta cttgtggcat gcaataagat tgaaactcct cacctgccaa
     6841 aattatacat agaaaattag atcctgatcg ttcgtaaaat taatgaaaca ggatatataa
     6901 tccaaatttt tacccccaag catgccaaag tttgtctgca aagactatgg ttttgaatgt
     6961 gattttgttg cagagggcga cgatattgca aaagtaattg ctgaatacag taaacattca
     7021 actgatgaac acggaatcga atactccaaa gaagctttga tgcagttcat aataagaaaa
     7081 ggctgaaaaa acaaaatttt ttggcaataa acttaacgca ataaaaaaag cttgggcttg
     7141 aatgtgtaaa agaatcccaa aaaacttgaa attctaatgg ttatccgtat gattcgtatt
     7201 ttacttcgaa gcttccatct tcacgtttgt cgtgcaaagt cacaaaacct tgcggcccca
     7261 aaaagtccat tactccatcg atggttcctt gccaaccgca aatgtagaaa ctcgtgttct
     7321 cttttgtaat tttttcccca actagttctt ctaatggtga ctttcctccg tcacgtggct
     7381 ttaggaaact ttcaactctg cctgtttgac ctttccatga tctgttgaac cattcctgcg
     7441 gacggctaat tgatgcacga tatctaaagt tccacttgtc tctacctctg tcaacactct
     7501 cttcttctaa tttagtaaat agtcccttgt aacttaactc gtctacataa ctggctccgt
     7561 gtaaaacaac aatctctctt ttatcgccaa tcgcacgaag gtgttgtgca aagcttacaa
     7621 aaggtgcaat gccagtgcct ccaccaatac agacgattct cctttcgtct tttctaccat
     7681 caggtaatct ttcgttaatt gttaaggcgt ttccagttgg ctttcccata gttatctgat
     7741 ccccctcact agcatagaat aactgagttg taacacgacc aggcaaaggc tttcgaaccc
     7801 atcgaagtac aaattcaaaa tattttttgt tttcgggatg agatgcaatt gagtacgctc
     7861 tgcgaaccgt cttgtagttt tcagctacta ctggaatgcc gatggtaacg aattgacccg
     7921 cctcatactc tggtatggtg ccatcatttg ggaccatacg taaaattaca agatcttctt
     7981 tgagtaattg aacataagaa atagttgcct tgttttctac taccatccta aatgatctgt
     8041 ctctgattgg ctaaatatct tttatgatgt ttccataatc atcaattaat ttgacaatgc
     8101 aatgtgtgat aaaggagatt tgtacccaat gactctaaca cagcgttgat ccaaaaaccg
     8161 acgcgcagac tacgtcgcat tgacacaatt cagattacct gagacaaaac ggcttcgttt
     8221 ccaattatta ccccatccaa ggtgactatt cttgcagatg aaacgtcttt gatcctttct
     8281 atgattggcg agattgatct tttgtactgc ttcttttttg ttgcataaaa gtatcgatta
     8341 aacgacggaa taattatgat ttccaactcc ccagcacttg acggaaatat gttctccttt
     8401 tttgtcttaa ttgataccca gacccgctgt ccatttagaa ccgagtcctc atcaaaaaag
     8461 acgggatgta catggcccat tattatctta tccacatgtg caaagttttc agatggaata
     8521 gtgtggccat gtgttagaag aacattttca agcaccaccc caattgcgct tatcagagtt
     8581 attccgtctg ggactaatcg ctgtatgtct gcgtcgtgat ttccaggaac tatgatgatg
     8641 tctgtttttt tctttaactc ctgcaaaaac tttggtatct catcccattc gttttttgaa
     8701 atcctgctta ttcctgactt tacatcgccc aaaagtatga tgtcgtctgg cttgttagag
     8761 tctacaaggg agtaaacctc atctagaaac tcgtttaggg tggtgttttt tccaacaaag
     8821 atcttgtttt gcgccaaatt gccttcaaag ccaatatgaa ggtcggttat gactaggtgt
     8881 tttttacccc cctccaaaat tagcgcaggc tttgatggaa ctatctttgt cgtcgccacc
     8941 aaaaatatag aatcgagttt gcattaaata cttgtggaaa aagtaaagaa aactgatgtc
     9001 ataatatccg aaaaaagagt caaacttcac atctttgaac caagcaaaag gcagatttgg
     9061 acagttgttg gaatgggcgg agaacactgg cttacccctg agtttgattt ttgttcatgc
     9121 cggggctact attttggaaa attcaaaaag aaaaagtcct gttaccatct tgaatcagta
     9181 aagatggcaa aaaaagaaaa tgacattgaa attgtaacct tctcagacga
//
LOCUS       DASWKH010000100         8737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1362851, whole genome shotgun sequence.
ACCESSION   DASWKH010000100 DASWKH010000000
VERSION     DASWKH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362851"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VGA92_07620"
     CDS             <1..459
                     /locus_tag="VGA92_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9846313.1"
                     /translation="GLSVAIGAFLAGVLVAESKSAEVSKILSSPIKDMFVAIFFVSVG
                     ALMDISELENYIFLAAGLVALTILVKFGGALAGAILFRQGKPKSLRSAFALAGPRGEF
                     SIVIIKTGVDVGAVSSFVFPLIGLISIISAFISPFLIRASDKIVPKITKE"
     gene            466..903
                     /locus_tag="VGA92_07625"
     CDS             466..903
                     /locus_tag="VGA92_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846314.1"
                     /translation="MSEDEMKKLMDRVHVGVTSFNYNGIKVPCLILSEKRFDQIMTQI
                     AGKPLSVETNLNILQDGHGHVFVEIVLTFSEGGIEEKLLLYANESLGFFESLASTSML
                     ALSSPHSEIGKDNVFMIQLPRPEKITNALEMIKQGLSSTNQTK"
     gene            complement(949..1098)
                     /locus_tag="VGA92_07630"
     CDS             complement(949..1098)
                     /locus_tag="VGA92_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEX9846315.1"
                     /translation="MSEERKMYPCTCADCGKQSEVPFEPKEGRPVYCRECLPKHRKPR
                     FQRRF"
     gene            complement(1182..1255)
                     /locus_tag="VGA92_07635"
     tRNA            complement(1182..1255)
                     /locus_tag="VGA92_07635"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1218..1220),aa:Gly,seq:gcc)
     gene            1373..1639
                     /locus_tag="VGA92_07640"
     CDS             1373..1639
                     /locus_tag="VGA92_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846316.1"
                     /translation="MDKRLEQKINEKIEEALANMDEIHNLAKSLDARGDSESFKYGLV
                     VGRLYNSFYYQSRRILKRDPTKKEFFEFLQILRKRENDILKTIS"
     gene            complement(1636..2136)
                     /locus_tag="VGA92_07645"
     CDS             complement(1636..2136)
                     /locus_tag="VGA92_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX9846317.1"
                     /translation="MHGQCYRRGNGQPYARKEFIKGKPQIKIAKFQGGKAGEYDFSVQ
                     LCSNEKIQIRHMAIESARLSANKKLEQTTGETGYYSILRIYPHILLRENKMIATAGAD
                     RLQEGMRRAFGKAVSLAARVKQGQCIMEMHVNKEHLDAAKKALQGACVKLPITPMIKV
                     TKLKKN"
     gene            2237..2812
                     /gene="endA"
                     /locus_tag="VGA92_07650"
     CDS             2237..2812
                     /gene="endA"
                     /locus_tag="VGA92_07650"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HEX9846318.1"
                     /translation="MSSDTEPNIEGMLVDDHTVITNKEMQNVLEQKGYGELKQNKFLL
                     KPFESMYFLFYNKLQLTKGKKRIGFDQMLQICTEFDENALTKFLIYRDLRVRGYAVKD
                     GFGFGSDFRVYERGQFGEKGAKYLVFGLAEGKQEKIGQLQKNIEEITKMGKEPILAVI
                     ERRGEIIYYKISLVRFVENTKNLDASDVVFS"
     gene            complement(2805..3479)
                     /locus_tag="VGA92_07655"
     CDS             complement(2805..3479)
                     /locus_tag="VGA92_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase"
                     /protein_id="HEX9846319.1"
                     /translation="MGLRDVSLKLQYRTDRDDLITDFFIPCLSNSIEYDRAVEFVTIK
                     SISTLSLGLQNFIQHDGKIRLVTGHRYRSSDLDILTKMFGKKNNNHNGVLHNGNTIHS
                     NKIEILHQLIQKNKIQIKVAIPNSEDVDGSFSERIGVFGDENGDAVAYTGTSNETFNL
                     ENRNFESVDVFTSWDEPSRVEIKKTDFENLWNNETKYVQVYDYSYASEHNLLKYDPRW
                     AIETIS"
     gene            complement(3526..4692)
                     /locus_tag="VGA92_07660"
     CDS             complement(3526..4692)
                     /locus_tag="VGA92_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9846320.1"
                     /translation="MSSNAISKKALLLHRKHKGKIAITSKIPNLKQKDLQLIYTPGVA
                     AVSKEIVSHPTQKYFLTSKANMVAIVTDGTRLLGLGNVGPYAAIPVMEGKAVLYKKFG
                     EVDAFPICLNTTKKEEIISIILAIEPVFGAINLEDIESPKVIEISEELEKRMSIPIFH
                     DDRHGTSVVVLAALLNSLKLVKKQLGSVKIIIVGAGSAGYGICKLLEFAGCKNIIVVD
                     SAGAIYKGRDKDMSKYKKEIAMITNPKKEGGSLSEVLKESDVFIGVSGVRNLLTAEMV
                     KSMNKNPIVFALTNPDPEINPRLAQKSGARIVATGSYQYQNKVNNALVFPYLMRAILD
                     HRIRKISLDLLLAASKAVARTVSGAKLSYNHIIPEIGDESLHKNISYAVAKFKK"
     gene            4769..5218
                     /locus_tag="VGA92_07665"
     CDS             4769..5218
                     /locus_tag="VGA92_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9846321.1"
                     /translation="MTHIKIRTATKNDILTILELLYEMQRPKPKTKSEKTKFTKLILQ
                     YLSEGDKKILVAYDDSKVVGLVSMMFLSRLNRIRKELYIPELVVAKKYRRLGVGNLLI
                     ESCIGIAKRKNCFRIRLESGKQRKEAHKFYQSLGFEQSALSYNFNLD"
     gene            complement(5267..5342)
                     /locus_tag="VGA92_07670"
     tRNA            complement(5267..5342)
                     /locus_tag="VGA92_07670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5304..5306),aa:Glu,seq:ctc)
     gene            5463..6347
                     /locus_tag="VGA92_07675"
     CDS             5463..6347
                     /locus_tag="VGA92_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846322.1"
                     /translation="MELQEIINQLSLSDYPVGLGGCKTHKTTLDCCEYNITVFDNKSS
                     KDEVHAFKDKIIKIHRGKLDESNPDILQKYDSMSVLSDNDWSLRIFLSKIKEKSEQIR
                     KSCIKSCLVDAASFATKAKQGLSSDPFAPAWLKCAAYFISDALVLMNSRPRSPTHMLE
                     FIRRFEKNQVNEFFSVVAEVIGLERTTPSLLERMVKSTIGFSDMTENNDHSKIISKKY
                     QYLVENSLLSDCYFYLGYINKNNIVSIMDSIHKRTDLIHVLKVAFDFENDPTRIEQQA
                     KSLHKLANDMTESLQKYA"
     gene            complement(6596..6748)
                     /locus_tag="VGA92_07680"
     CDS             complement(6596..6748)
                     /locus_tag="VGA92_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846323.1"
                     /translation="MFEVEFKLAGMSVVPTHKNCGNALSETQADKFQKELVKSWGLDP
                     EDENPI"
     gene            6856..8559
                     /locus_tag="VGA92_07685"
     CDS             6856..8559
                     /locus_tag="VGA92_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEX9846324.1"
                     /translation="MRKSGIFVALLAVTILTFAFSQNAFAQFQGGGVSKEGTWYVGEG
                     LKKGDQFSYSLCHIDHKDCSQFKIDFWVEGDVQVGSETQWKVQTVVHDGAKIFKGTMN
                     LGKLAPEPTSSTPNIAEFAAAFKSSIVWMSAYATADPTSNSIKGPKQFSAPSWGKIGN
                     IGGEQIIPTAEEKIAVPDGVFDTVQVSWKTGGKINRVWVVDEFPFPIKADTYAHVSEG
                     VPPQEYRFELLDYQQNVKTNPFTNIQSTPDAQAGLGCQEIYDLTRVSKLTNTNTMIVE
                     INYGPPQPKQGCDLELIINFKRMVHETEFENEIHYDILVVEQTSAGLSKKRSIADEEN
                     RDALFTTSGQVRRSIIVNESGPTTYAIFVYGTGSEYLQPNPAKAGYMTFDVNVQKGTI
                     TSTPPPIECPLGQILSGGQCIDEQSEGTGPECDPGTVAKDGICVPVVEAKPPTNGCLI
                     ATAAFGSELAPQVQLLREVRDNVLFSTSSGTSFMTGFNSIYYSFSPTVADWERNNPVF
                     KEVVKTAITPMLSTLSILNYVDINSEQEMLGYGIGIILLNIGMYFVAPVIVIVKLRSL
                     N"
BASE COUNT         2642 a         1755 c         1798 g         2542 t
ORIGIN      
        1 ggcctatcag tggcaattgg agcgtttttg gcaggagtgc ttgtagccga atcaaaatca
       61 gctgaggtct caaaaatttt gtcgagtcca attaaggaca tgtttgttgc aatatttttc
      121 gtctcagttg gagcgctcat ggatatctcg gagcttgaaa actatatctt tttggcagca
      181 ggactagttg cccttaccat cttagtaaag tttggaggtg ctttggctgg cgcaattttg
      241 tttaggcaag gtaagcccaa atcgctgcgg tcggcatttg cactggcagg tccaagaggg
      301 gagttttcca tagtgatcat aaagactgga gtcgacgttg gagccgtgag cagctttgta
      361 tttccgctaa tcggcctgat atctataata agtgcattta tttcaccatt cttaattaga
      421 gcctcagaca agattgttcc aaagataaca aaggagtaaa aaaaattgtc agaagatgaa
      481 atgaaaaaac taatggatcg agtgcatgtt ggcgttacgt cgtttaacta taacggaata
      541 aaggtgccgt gcttaatttt gtctgaaaaa cgatttgacc aaataatgac acaaattgca
      601 ggaaaaccgc tttctgttga aactaacctt aacattttgc aggatggcca tggccacgta
      661 tttgttgaaa ttgttttaac gttctcggag ggaggaatcg aggaaaaact cctcctatat
      721 gcaaacgaat cacttgggtt ttttgagtcg cttgcaagca cttcgatgct ggcactctcc
      781 tcaccacatt ctgagatagg caaggacaac gtctttatga ttcagctccc acgccctgaa
      841 aaaataacaa acgccctaga gatgataaaa cagggactga gctcaacaaa tcaaactaaa
      901 taatacaact agcggaaatt gaataaaaaa cgaagtttaa gcaatcgctt aaaatcttct
      961 ttgaaatctt ggttttctat gctttggtaa gcattcacga caataaacgg gccttccttc
     1021 ttttggttca aagggtacct cagattgttt gccgcaatca gcacatgtac acgggtacat
     1081 ttttctttct tctgacatgc ttgtaatttg atttttttta gtgatatatg aatcaaaggg
     1141 taggtttgac gccttgcatc tcaaaaatca tcatgaaaag atgcggctgc cgggatttga
     1201 acccgggtca cagaattggc aatcccgcgt actggccagg ctatactaca gccgcatgat
     1261 cgattacaca aaaccaagtc tattaaaccg taacgattcg gatcgtatgc aaaaaaaact
     1321 tgatgcattg tatgactatc acctttaatc ctagatcaat gctatgtgtg atgtggacaa
     1381 aagactggag caaaaaatta acgaaaaaat tgaagaagct ttggcaaaca tggatgaaat
     1441 acacaatctt gccaaatctc ttgatgccag gggggattcg gaatccttca agtacggtct
     1501 agtagttgga aggctgtaca attcatttta ctaccaaagc agaaggattc taaaacgcga
     1561 cccgacaaaa aaagagtttt ttgagttttt gcaaatactg cgtaaacgag agaatgatat
     1621 tctaaaaacg atatcctagt ttttctttaa tttagttacc ttgatcatcg gagttatggg
     1681 taatttgaca caggcgcctt gtaatgcttt ttttgctgca tcgaggtgct ctttgttcac
     1741 atgcatttcc attatacact gtccttgttt tacacgtgca gcaaggctca cggccttacc
     1801 gaacgcacgc ctcattccct cttgaagtct gtctgcaccg gcagttgcaa tcatcttgtt
     1861 ttcacgaagt aaaatgtgcg ggtatatcct tagaattgaa tagtatccgg tctcccccgt
     1921 tgtctgctct agtttcttgt ttgcagaaag tcttgctgac tcgattgcca tgtgtcttat
     1981 ctgtattttc tcatttgagc acaactgcac gctaaagtcg tattcgcccg cttttccgcc
     2041 ctgaaacttg gctattttta tctggggttt tccttttatg aactcctttc ttgcgtatgg
     2101 ttgtccattt cctcttctat aacattgacc gtgcataata tcaccgatga tgccagatgt
     2161 aaaacacctc tattttaacg gttagacagg atttgagtac tacgctttat attgaaaatg
     2221 gaataatttt ttccaaatga gctcagatac agagccaaac attgagggaa tgctagtaga
     2281 tgatcataca gttattacaa acaaggaaat gcaaaacgtc cttgagcaaa aaggctacgg
     2341 ggagctaaag caaaacaaat ttctcctcaa gccgtttgag tctatgtatt ttctttttta
     2401 caacaaactg cagcttacca aaggaaaaaa gcgcataggc tttgatcaaa tgttgcaaat
     2461 ttgtacggaa tttgatgaaa acgccctgac aaaatttctg atttatagag acttgagggt
     2521 aagggggtat gccgtaaagg acggttttgg atttggttct gactttaggg tttatgagcg
     2581 gggccagttt ggcgaaaaag gcgccaaata cttagtcttt ggcctagctg agggaaagca
     2641 ggaaaaaatc ggccagcttc aaaaaaacat tgaagagata acaaagatgg gaaaagagcc
     2701 aattttggca gtaattgaaa gacggggcga gataatctac tacaagatct cgcttgtgag
     2761 gtttgtagag aacacaaaaa accttgatgc gtctgacgtt gtgtttagct aattgtctct
     2821 attgcccatc tgggatcata ctttagcaaa ttgtgctcag acgcatatga atagtcatac
     2881 acctgaacgt attttgtctc attgttccac aagttctcaa agtcggtttt tttgatttct
     2941 actcttgatg gttcatccca ggaggtaaag acatcaactg actcaaagtt tcggttctcc
     3001 aaattgaaag tctcatttga tgtgcccgtg tatgcaactg catcgccgtt ttcatcgcca
     3061 aagaccccaa tccgctcaga aaaagagcca tctacgtcct cggagtttgg tatggctacc
     3121 tttatctgaa ttttgttttt ttggattagc tgatgcaaaa tttcaatctt gttgctgtga
     3181 atagtgttcc cattgtgcag aactccatta tggttattgt tttttttgcc aaacattttg
     3241 gttagaatgt caagatctga tgacctgtat ctgtggcctg tgactagtcg tattttgccg
     3301 tcatgttgga taaaattttg caggccaaga gacaaagtcg agatgctctt tatcgttacg
     3361 aattcgactg ccctgtcata ctctattgaa ttgctaagac agggaatgaa aaagtctgta
     3421 attaggtcat ctctgtcagt tctgtattgt agctttagag aaacgtctcg cagtcccaat
     3481 aaattttaga gcactttatc ttatttaaat gaaaatttgt ctttgctatt ttttgaattt
     3541 ggctactgca tagctgatat ttttatgcaa agactcgtcg ccaatttctg gaatgatgtg
     3601 gttgtaactg agttttgcgc cgcttacagt tcttgctact gcttttgatg cggcaagcag
     3661 caagtcaaga ctaattttcc ttattctgtg gtccaaaatc gccctcatca aataaggaaa
     3721 gacaagtgca ttgttgacct tgttctgata ctgataactt ccagttgcaa caattcttgc
     3781 accgcttttt tgtgcaagcc ttggatttat ttcaggatct ggatttgtta gcgcaaaaac
     3841 tatgggattt ttgttcatgc tttttaccat ttctgcggtg aggagatttc ttactccaga
     3901 tacaccaatg aagacatcag actctttcaa aacttcggaa agtgaacccc cctccttttt
     3961 cggatttgta atcattgcaa tctctttttt gtatttgctc atgtctttgt ctcgtccctt
     4021 gtaaattgca ccggcagaat ctacaacaat gatgttttta catcctgcaa attctagcag
     4081 cttgcagatc ccatagccgg cagagccggc acccacgatt attattttga cagaaccaag
     4141 ttgttttttg accaatttta gggaatttaa caaggctgcc agaacaacaa cggatgttcc
     4201 gtgtctatca tcgtgaaata ttggaattga cattcttttt tccagttctt cagagatctc
     4261 aattactttg ggagactcta tatcctcaag atttattgcg ccaaagactg gctcgattgc
     4321 caaaattatt gaaatgattt cttctttttt tgtcgtatta agacaaattg gaaacgcatc
     4381 tacttcacca aactttttgt acaaaactgc ctttccttcc atgactggga tggctgcata
     4441 ggggccaaca ttgccaagcc caaggagcct ggttccatcg gttactatgg ctaccatgtt
     4501 tgcttttgag gtaagaaaat acttttgtgt gggatgcgaa acaatctctt tgctgactgc
     4561 agctactccc ggcgtgtaaa ttaactgcaa gtctttttgt tttagatttg gaatcttgct
     4621 tgtaatcgct atcttgcctt tatgttttct atgtagaagc aatgcctttt ttgaaattgc
     4681 atttgaagac atgcactgat caacggaaga cttcatttaa agaatgctaa gtgcataact
     4741 tttaacgcgc gcaatgacat tgctaagggt gacacatatc aaaataagga ctgctacaaa
     4801 aaatgacatt ttgacaattt tggagctgct ttatgagatg cagcgaccaa aaccaaaaac
     4861 caaatcggaa aaaaccaagt ttacaaaact aatcttgcaa tatctgtctg agggggacaa
     4921 aaaaatccta gtggcatatg atgattccaa agttgtcggc cttgtcagca tgatgtttct
     4981 ttcaaggctt aatcgtataa ggaaagaatt gtacattccg gaactcgtcg ttgcaaaaaa
     5041 gtatcgaagg ctgggcgtgg gtaatctgtt aatcgaatcg tgcattggta ttgcaaaaag
     5101 gaaaaactgc tttagaatac gactggaatc gggaaaacaa agaaaggagg ctcacaaatt
     5161 ttaccaaagt cttggctttg agcaatctgc actaagctac aactttaatt tagattagga
     5221 aatttctaaa gccttgattc gcaaactctg ctaaaccaaa aagacatgct cccgccggga
     5281 tttgaacccg ggatcactgc cttgagaggg cagtatgctt aaccggacta caccacagga
     5341 gcttaaaaaa agcggtaaat gctagcaatt taaaggaatg gctacggtgt ttcaatattg
     5401 gattgtttgg ttaaaaaata atcattggaa cacttaagaa aattaaagag caaatttctg
     5461 atgtggagct acaagaaatc ataaaccaat tatcattatc tgactacccg gtggggcttg
     5521 gaggctgtaa aactcacaaa accacacttg attgttgcga atacaacatc acagtttttg
     5581 ataacaagtc atcaaaagac gaagttcacg cattcaaaga caaaataata aaaatccatc
     5641 gcggcaagct tgacgaatca aatccagaca ttttgcaaaa atatgactcg atgtccgtat
     5701 tatcagacaa cgattggagt ctccgaatat ttttatcaaa aattaaagaa aaaagtgagc
     5761 agataagaaa atcctgcatt aaaagctgtc ttgttgacgc ggcatcattt gcaaccaaag
     5821 caaaacaagg gctgagctcc gatccatttg cacctgcttg gctcaaatgc gcagcttact
     5881 tcatatctga tgcactagta ttgatgaact caaggcctcg aagcccaaca cacatgctgg
     5941 agtttatcag gaggtttgaa aaaaaccaag tcaacgagtt cttttctgtc gtagcagaag
     6001 taattggcct tgagcggaca acaccatcgc ttcttgagag gatggtcaaa tcaacaattg
     6061 gattttccga catgacggaa aacaacgacc attccaaaat aatctcaaaa aaatatcaat
     6121 acctagtaga aaactcgctt ctttccgact gttactttta cctaggttac ataaacaaaa
     6181 acaacattgt ctccataatg gattcaatac acaaacgcac agatctaatt catgttctca
     6241 aggtcgcctt tgattttgag aacgacccca ctagaataga gcaacaagca aaatccttgc
     6301 acaagctggc aaacgatatg acagagtctt tgcaaaaata tgcctaaaca tcttttcaat
     6361 atgatctgga taacctttaa aactatgtga taggatccct actttatggt agatcaagct
     6421 tgcggatgcg aagcagaaag cggtgatcct gactttaaat gtgattgtga aatgcaagga
     6481 cactgtattt gcagccacga ttgtagatgc aaatcatccg tttgtaaaga agcagtctcc
     6541 gagttaagtt aaaaatactt aacattctat tttttgtttt atttttcgtt tggccctaaa
     6601 ttgggttttc gtcctcagga tccagtcccc aagacttgac aagctctttt tgaaatttgt
     6661 ctgcctgtgt ttcgcttagt gcattgccac agtttttgtg agttggaaca acactcatgc
     6721 cggccagctt aaactctact tcaaacatgt gaatcttggc gcaatcgcac attctaattt
     6781 tcgttcgaaa ttcctctata tttgcgtagc ttgactgaat aacttttaac tagggttttg
     6841 actaaaatag atcacttgag aaagtctgga atttttgtcg cattactagc agtaactatt
     6901 cttacatttg ccttttcaca aaatgccttt gcgcagttcc aaggcggtgg ggtaagcaag
     6961 gaaggcactt ggtatgtggg agaaggactc aaaaaaggag atcagtttag ctattcatta
     7021 tgccatattg atcacaagga ctgctcgcaa tttaagatcg atttctgggt agaaggcgac
     7081 gtgcaagttg gaagcgaaac ccagtggaaa gtccaaacag tagtgcatga cggcgcaaaa
     7141 attttcaaag gaacgatgaa ccttggtaaa cttgcaccag agccaacaag cagcactcca
     7201 aacattgccg agtttgcagc cgcattcaaa tcctcaatag tttggatgtc tgcatatgct
     7261 acggcagacc cgacatccaa ttctataaag ggaccaaaac agttcagtgc cccgtcttgg
     7321 ggaaaaatag gtaacattgg aggcgagcag atcataccta cggcagaaga aaagattgca
     7381 gttcctgatg gcgtatttga taccgttcaa gtctcatgga aaaccggagg caagataaac
     7441 cgggtttggg ttgtagacga gtttccattt ccgataaagg cagacacata tgcacatgtt
     7501 tctgagggtg tgccaccaca agaatacaga tttgaattat tagattacca gcaaaacgtc
     7561 aagacaaacc catttacaaa catccaatca acaccagatg cccaagcggg tcttggttgc
     7621 caggaaatct atgacctaac gcgagtctca aagctaacca acaccaacac tatgatcgta
     7681 gagatcaatt atggcccccc acaaccaaaa caaggatgcg acttggaatt gataatcaac
     7741 tttaagcgaa tggtacacga aaccgagttt gaaaacgaaa tccactatga tatactggta
     7801 gtggagcaaa cttcggccgg gctttctaaa aaaaggtcga ttgccgatga agaaaatcga
     7861 gacgcactct ttactacgtc tggccaagta aggcgcagca ttatagtaaa tgaatcaggc
     7921 ccgactacat atgcaatctt tgtgtatggt actggatctg agtacttgca accaaatcca
     7981 gccaaggcag gttatatgac atttgatgtt aatgtacaaa aaggcactat tacttccact
     8041 ccgcctccaa tagaatgtcc actaggacaa atactatctg gcggccaatg cattgacgag
     8101 caatcagaag gcacaggtcc agaatgtgat ccaggaacag tagctaagga cggcatttgt
     8161 gttccagttg tggaagctaa accaccaaca aatggctgtt tgattgcgac tgctgcattt
     8221 ggtagcgagc ttgcaccaca agtacaacta ctcagagaag tcagagacaa cgtactgttt
     8281 agcacaagtt ctggaacaag cttcatgact ggctttaact caatctacta ctcatttagc
     8341 ccgactgttg cagactggga gagaaacaac ccagtattca aggaagtagt caagactgca
     8401 attacgccaa tgctttcgac tttatcgata cttaactatg ttgacatcaa ctctgagcaa
     8461 gaaatgctcg gatacggaat tggcatcata ctgctaaaca ttggaatgta ctttgttgcg
     8521 ccagtaatag tgatagtcaa acttagaagc ctaaactaat ttagctgatc ttggtcaaaa
     8581 aaattctctt cattccatta gggataatgt ttggagctgc tgcaataggc cttgcatatc
     8641 cgtttttgac tggtgtgccg ggagctgaac aaaacttaat cccaagattt gcaagccagt
     8701 ggcacgtggg aaagggcgca gaagacaaac cgacgtt
//
LOCUS       DASWKH010000101        16174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_256670, whole genome shotgun sequence.
ACCESSION   DASWKH010000101 DASWKH010000000
VERSION     DASWKH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 16174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16174
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256670"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VGA92_07690"
     CDS             <1..507
                     /locus_tag="VGA92_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9846325.1"
                     /translation="EINAAEADVLLRLDEALDDRRQIVLKESTGEIIKADEKWFVIAT
                     INPLTHVGTKELPPQLLSRFPVRIRLDYPPEQTELQIVKRYVSGNHDDILLQGIKLAN
                     TLREAASVEELYYSPSLRETIAFGRLLDAGVDSKKAANIVFANVYAQWGNVEYQKVND
                     IITSMFGS"
     gene            507..2072
                     /locus_tag="VGA92_07695"
     CDS             507..2072
                     /locus_tag="VGA92_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX9846326.1"
                     /translation="MQSLHLRNDTLVEIATFLARRWSQKEKLTVEFSDKKENQTKLKE
                     NRISLIAPERYLGSDFERYRQFRTSIWYEAMRAKHCKKILSNDHAFGFILNTLETRRV
                     ELLGRKIWRGMDEELIFSYSYWWLYRPQLANVLGKTRIVEAFYQYFLFGDIKGELQPS
                     YHDKIRKASARSKEIIAEALQKNHDTEWLEKKIPEILSILEIDSLVTIPLSTPWKGPG
                     LLVTPEDLLKAMQKIAKNRESDFGKIDVKNIMSGKAVAEEFKVIKEETRKNENKGLGN
                     ETIGIQVPGSTNVDETRIYDQDLISNLKTRFKDWKTSWSEQHVSSGDEFDEESYIEGH
                     LPFLTDIKKSIKAKIVILVDHSSSITDQQTQYKKVTVALCEVLSYLKIKFSVYAFNTT
                     QKQVVCWLVKPEDMKWNNACAKRLAQIDANGGTPLADVYQKMYPILRSKKPDIFLTLS
                     DGEPSDPDAVRAMVKSLKTVGIKMVAIGVGRDTFGATQIANNLKYLEYERTLAVSRLK
                     DIPNRVLAVLQTS"
     gene            2161..2586
                     /locus_tag="VGA92_07700"
     CDS             2161..2586
                     /locus_tag="VGA92_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846327.1"
                     /translation="MANKMQKILVPLDGSQNSTRALEKAITIAQPTGATITGLYVIHI
                     PARSAIRFTPQQRKKEISFAESIISEAAEKVERAQVNFKPRTETGDPGQKIAQVAKDS
                     IYDLVVIGSRGRGTGKEMFLGSVSNYVLHKTNIPVMVVK"
     gene            complement(2631..2861)
                     /locus_tag="VGA92_07705"
     CDS             complement(2631..2861)
                     /locus_tag="VGA92_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846328.1"
                     /translation="MQYTVKAKICTVVGIGLIVFVASLVGLPFTDSESNVKKISYENA
                     KKLDDSEQKMLDVIAQEKAEIESKIGGKINFP"
     gene            complement(2970..3497)
                     /locus_tag="VGA92_07710"
     CDS             complement(2970..3497)
                     /locus_tag="VGA92_07710"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9846329.1"
                     /translation="MNVINNVDLDKVSQTIEGGKKDRTTLKKPVKLQGEWNFDPSKGY
                     QFRTELAFEKGKQLIEIDSPSFLGGGGNRLGPMAYCVAGITSCFVGTFVGIAASQGIR
                     LTKLSVNTECGVNFAKTFDVSDEPITEGINFQIDAQAENADRQKLQELVKMAEERCPA
                     VYSMSNIIKVNAQIK"
     gene            complement(3521..4318)
                     /locus_tag="VGA92_07715"
     CDS             complement(3521..4318)
                     /locus_tag="VGA92_07715"
                     /inference="COORDINATES: protein motif:HMM:NF019949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FilR1
                     domain-containing protein"
                     /protein_id="HEX9846330.1"
                     /translation="MKEESLPFELFTDLASETRCAILVALSKEPLRLNQLAKKLDLTI
                     QDAHRNADKLAGSSIIQKNADATLSLTEYGRLMAKQIQYFEFLYRYKQFFEDHIIQNM
                     PEKFVQRIGALQNSQLITSVTEVLEKLKKIESNTKKALKIMVSQAWSAEGKILINLTK
                     NDVIVHTLIGKNTIFPDEIIDTIIKNLDKLSSKENFVQRQIDSVDVAVYITDDSCAVM
                     FPNKKGQIDMNSMFLSEDDSFREWCNDIFEHYWSRSRPQVRISKKQI"
     gene            complement(4369..4797)
                     /locus_tag="VGA92_07720"
     CDS             complement(4369..4797)
                     /locus_tag="VGA92_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846331.1"
                     /translation="MKTNHKSILALAGVAALVALLSVPVMSENAEPQAKAASDVAPVL
                     QPRTVIVAGNEDPQFGPKQLTITAGEEVKFFNVDGFNGVGRVHQIVSVDAQSGIPNGV
                     FDTGVLSRGGSATIKITEPGVYGFIDQLWPATQGTITMVP"
     gene            4922..5653
                     /locus_tag="VGA92_07725"
     CDS             4922..5653
                     /locus_tag="VGA92_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9846332.1"
                     /translation="MKNKIIHSSEHRTLEHDWLRTTHHFSFGEYYNPDRMSFGPLRVF
                     NDDVIKPGAGFDFHQHRDMEIVTYVIDGALEHKDNFGNHDVIGSGEIQRMSAGTGVYH
                     SEYNHSQKGPLRLLQMWVFADHKDLKPSWEQQKFTKHERQNKLLAVISPHKSGKKTLS
                     IHQDASFYISNITQDHTVDHTLAQKRQAYLYVIKGTVKVNGQSLNQGDSAEIQDSDIS
                     MTAANDSEIILIDLPQQYVKNSETS"
     gene            5920..7323
                     /locus_tag="VGA92_07730"
     CDS             5920..7323
                     /locus_tag="VGA92_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HEX9846333.1"
                     /translation="MTKYLVIPIVVLIFISYSINGVSAQEILSPREQTKMGVDPNNVQ
                     CHDGLQLVLRSNVMPACVRPATAEILNQRGLILFMELVPAPIEPPPTEKPEPSGKEAS
                     DIIEASNEFMLDFYSLNNAGQDNIFFSPWSILSAFSVVYEGARGQTAEEVASATYLPR
                     DDSDRRDSFKTIQQNLNPNSTEYELTNANALWVKTGFGVKQEFVDIAKQYYDSEVAEV
                     EFPAGESEIDSWVENQTNGKIKDLVKGKTNDMTRLVITNAVYFMGTWKDQFNANLTSQ
                     DDFTLDSGDTVEVPMMYQKSRFDYAENDNLQILSMPYKGDRLSMLVLLPKNNLGSLED
                     SLTLEELSSWQEQMQNKEVYVYMPKFKLEAEYDLSPQMKELGVELAFDPDYADLSGIA
                     DVKPNNLYIGFATHKAFVDVNEEGTEAAAATAIGIGVTSAPVDPTVFRADHPFVFLIQ
                     DNETGLVLFMGRVSDPS"
     gene            7368..8771
                     /locus_tag="VGA92_07735"
     CDS             7368..8771
                     /locus_tag="VGA92_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9846334.1"
                     /translation="MDSIEITPEELSQIIDNKIAALILDIRAKEKYMDGHVPGAANAV
                     CQNMQQKQVIMSKIPPSMKIILIDEDGTESKQNAIMMARFGFDAHYLKDGIKSWNKEL
                     VKSSWDVTISGDNLWDSLKQDKDVFLLDVREPEEFADFKIPGAVNIPLSELFSPAVES
                     SIPKDKKIVTICSHGNRSMVATFALAQKGIEASSLVGGMSLWNQVLNATTLKENDLTV
                     IQVEKVGKGCLSHIVGSGGEAAVIDPTYPPSKYVEFAQSEGLKITKVIDTHQHADHVS
                     AAKDLAQLAGAKLYLSKLEEYKIDSERIGNGDSIPFGSKQLKVIHTPGHTAGSMSYVV
                     DDRYVFSGDTLFVEGVGRPDLRDQVQEYATSLYDTLHNKILKFSDYTKVFPTHHSEGI
                     KPTENGIYYTTVKMAKSLPLLDLSQPDFVAKVISITTPRPMNYSMIIKVNKGTIPISP
                     MQIPDLEMGPNRCSIRM"
     gene            8773..9240
                     /locus_tag="VGA92_07740"
     CDS             8773..9240
                     /locus_tag="VGA92_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX9846335.1"
                     /translation="MTAFKCLSVSQPFADLIVKGKKTIELRSWNTSYRGEFLVHAPIK
                     IRTSDCKRLGIDQASLPVGAIIGKATIHDVKLYQTRKEWQADQKYHFADCDFSGSRYG
                     FLLKGAKSFVIPIPCKGKLGFFDVQLDSTKVKDADITSEIFDEEYRTQWINHH"
     gene            complement(9237..9518)
                     /locus_tag="VGA92_07745"
     CDS             complement(9237..9518)
                     /locus_tag="VGA92_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009628326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9846336.1"
                     /translation="MKATWNDTILAESDQTIIVEGNHYFPYDSIKKEFFVKSDTTTIC
                     GWKGMANYFSVVINGKTNKDCAWYYPEPNDAAKKIKNHVAFWNGVKVSS"
     gene            9630..9779
                     /locus_tag="VGA92_07750"
     CDS             9630..9779
                     /locus_tag="VGA92_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846337.1"
                     /translation="MKLFRRNSTQDMSPVCKICGMEFTEVERMMRHMTKAHSKPCKGG
                     SCGCS"
     gene            complement(9789..10811)
                     /gene="egtD"
                     /locus_tag="VGA92_07755"
     CDS             complement(9789..10811)
                     /gene="egtD"
                     /locus_tag="VGA92_07755"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9846338.1"
                     /translation="MVQRKMKPHSDSNYVRTVLSPNCTLIKSLEKKEQDFAKDVAYGL
                     TRKEKFVPSKYFYDEAGSELFDKICDLPEYYLTRAELEILDSAKQELSEYLDGNYSVV
                     ELGSGSAVKTRHLFEVLCKSQKKIAYYPIDISNVVKESSQRLQGEFDNLQITGIVDQY
                     ESGLDLVKDVAGKKIIAFFGSSLGNFDQSSAQDFLRKVRSSMNSGDLFLLGVDLVKGK
                     KVLEAAYNDSEGVTRDFNLNLLRRINYELRGDLDYAKFEHVAFYNSKEKRIEMHIRSK
                     TKHQVSIQDIGLSVDLEKGETIRTEYSHKYAISQIRKMAKKAGLSPVKIWRDEKNYFA
                     LVLFSV"
     gene            10925..12250
                     /gene="egtB"
                     /locus_tag="VGA92_07760"
     CDS             10925..12250
                     /gene="egtB"
                     /locus_tag="VGA92_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX9846339.1"
                     /translation="MNTKKPLHIEMLSTYYLVEEFKATRSTTLRLVDTLEGDDFAVQT
                     DDYVSPPKWHLGHVSWLFEMLLQQASKDYRVYSTEFLKYLNSYYNKFGEQHEKAQRGS
                     VARPTTGQILEYYDIISKQVINFLETNPANDSQRKLFELGIHHECQHQELLVYDIQHI
                     LGDKYRPSIKKQAPKPHAKQKQIHVEGGLYNLGYSGKDFCYDIELPEHKVYLNDYKID
                     ALPVSCGQFIEFINSGGYQDYRYWLSDGWDAVQKNKWESPMYWYKIDGDWYVDDFLGR
                     RKINPNEPVCHVSFYEADAYCRWAGRRLPTESEWEKAACWNEEKQIKTIFPWGNESPS
                     EKHANLLESFLWGCSEIGSYPDGKSHYGCHQMIGDVWEWTSSEFVGYPGFKSGFSEYN
                     DKWFTNQKVLRGGSFATPQISIRGSYRNFFRLDERWLFSGFRCVENIRI"
     gene            12596..12778
                     /locus_tag="VGA92_07765"
     CDS             12596..12778
                     /locus_tag="VGA92_07765"
                     /inference="COORDINATES: protein motif:HMM:NF037845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846340.1"
                     /translation="MSSGVSGDDDEVLVRYKFIGTHKASEIRCTYLQYRNLKMLPNIE
                     FCTIVKDEDDSEKNPS"
     gene            complement(12853..13176)
                     /locus_tag="VGA92_07770"
     CDS             complement(12853..13176)
                     /locus_tag="VGA92_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846341.1"
                     /translation="MAKSRRSKAKRKNKRRSSRTARNLTRAKKPAKKRPAKKKTRRHT
                     RTKRSKKGTKRKAKSKKTKSKAAKNIASILSKIVMLLDKPVMSQEEKDIIKDSIIAVS
                     KFIRS"
     gene            13339..14184
                     /locus_tag="VGA92_07775"
     CDS             13339..14184
                     /locus_tag="VGA92_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9846342.1"
                     /translation="MAKTRSKKLAKKRIRYASKAPYRLKEIPIKQIRVWKEAQARKLD
                     REGISELARSIKNEGLQNPPMLQKEGKNTYLLMSGQRRLAALKRLGAKKIPALVLTKK
                     TEYEVPDAKAASVIENIHRKNMNVKDMAAACAFLADQMGKSKAAQSLGLSMSTFKKYH
                     GFAGVPDKLKELVPRIISRDDATKLYQIIPNVSKSVKIAHKISGLDTATKKRYLKVLV
                     RNPHSPHKKILKKARSMTVQQKISLRLSKRQARGLGTQSRHQDVDPNELAGKIISDWL
                     GRKGY"
     gene            14427..15032
                     /locus_tag="VGA92_07780"
     CDS             14427..15032
                     /locus_tag="VGA92_07780"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9846343.1"
                     /translation="MKLHKYLEGVLGNKGAISVLRALVNHRGRTYTIRRLAKDAGISH
                     TETSATIQDLEKLGIVQVQPIGKAYNISLNEKSYVLANIIEPVLDVEKKSLDQVISIC
                     KKHLSSKKIISAVIFGSVARGEEKEDSDIDLLIISNDFDKAIEIVSRLSEEMSLAFHT
                     RISHITFSEKKLRSKRNSDLIRSIIKEHILVSGKELEDLIK"
     gene            15029..15253
                     /locus_tag="VGA92_07785"
     CDS             15029..15253
                     /locus_tag="VGA92_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846344.1"
                     /translation="MTKPVDPKKSRNYIIKAENSLRIAKIAIEHNAYDNAVMTAIHSA
                     INALDALTSSEKGLGPQVNTMRFSHFSRAF"
     gene            15496..16059
                     /locus_tag="VGA92_07790"
     CDS             15496..16059
                     /locus_tag="VGA92_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HEX9846345.1"
                     /translation="MPQTKPIISVENVVASASVDQKIDLNEVTKKFPDTEYHPDQFPG
                     LVFRLKTPRTATLIFRTGKMVCTGAKSEEMAIKAVQTVVDRLRKGGIKIKKDAEIVVQ
                     NIVAAINLGGKIHLEHAARTLPRSMYEPEQFPGLIHRMLDPKTVILLFASGKLVCTGA
                     KKEADVYRSVNNLHSLLEEKGLMIYDQ"
BASE COUNT         4978 a         3428 c         3489 g         4279 t
ORIGIN      
        1 gagataaacg cagcagaagc agacgtcctg ctaagacttg atgaggcact agacgacaga
       61 agacagattg tcctaaagga atcaactgga gagatcatca aggcagacga aaaatggttt
      121 gtcattgcaa caattaatcc ccttacacat gtcggcacaa aggagctccc gccgcagctg
      181 ctaagcagat tcccagtcag aattagactt gattatccgc cagaacaaac agaacttcag
      241 atagtaaagc gatatgtctc tggaaatcac gatgacatac tactccaggg aataaagctt
      301 gcaaacactt tgcgagaggc cgcatcagtt gaggaattgt attattcacc aagccttcga
      361 gagacaattg cgtttggcag actgcttgat gcaggagtcg attcaaaaaa ggcagcaaac
      421 attgtctttg caaacgtgta tgcacaatgg ggaaacgtag aatatcaaaa ggtaaacgat
      481 atcatcacat cgatgtttgg tagctaatgc agtcactaca ccttagaaac gacacactag
      541 tagagattgc cacgtttttg gcaagacgat ggtcacaaaa agaaaagctg acagtggaat
      601 tttctgacaa aaaagaaaac cagacaaagc taaaagaaaa cagaattagc ctgattgccc
      661 ctgagcggta tcttggcagc gattttgagc ggtatcgcca atttaggaca tcaatctggt
      721 atgaggcaat gcgcgcaaaa cactgcaaaa aaatcctaag caatgatcac gcgtttggat
      781 tcatcctcaa cacgctagag acgcggcgag tagagctgct tggaagaaaa atttggcgtg
      841 gaatggatga ggagctaatc tttagttatt catactggtg gttatacagg ccgcagcttg
      901 caaacgtctt gggaaagaca aggattgtgg aggcgtttta ccagtatttt ttgtttggcg
      961 acatcaaagg cgagctccag ccaagctacc atgacaaaat acgaaaggca tctgcccgct
     1021 caaaggaaat catagcagag gcgctgcaaa aaaaccatga cacagagtgg cttgagaaaa
     1081 aaattccaga gattctgagc attttggaaa ttgactcgct agtcaccatc ccactttcta
     1141 ctccatggaa gggcccaggg ctactagtta cgccagagga tcttctcaag gcgatgcaaa
     1201 agattgcaaa gaatagagag tcagattttg gaaaaatcga tgtcaaaaac atcatgtctg
     1261 gcaaggcagt ggcagaagag ttcaaggtca taaaagaaga aacccgcaaa aacgaaaaca
     1321 agggcctagg aaacgaaaca ataggaattc aggtcccagg ctctacaaac gtggatgaga
     1381 ctcgaatcta cgatcaggac ctaatcagca atctcaagac gcgattcaag gactggaaga
     1441 ctagctggag cgagcaacac gtatcatctg gtgatgagtt tgatgaggag tcatacatcg
     1501 aaggccacct gccgtttcta accgacatca aaaaatcaat caaggcaaag attgtcatcc
     1561 tagttgatca ctcatctagc atcacggacc agcagacgca gtacaaaaaa gtcacagttg
     1621 cactctgtga ggttttgtca tatttgaaga taaaattctc agtgtatgct ttcaatacaa
     1681 cacaaaaaca ggttgtgtgc tggctagtca agccggagga catgaagtgg aacaacgcgt
     1741 gcgcaaaaag gcttgcccaa attgacgcaa atggcggaac gccactagct gacgtgtatc
     1801 aaaagatgta tcccatttta agatcgaaaa agcctgacat ttttctgacg ctatctgatg
     1861 gcgagccatc agaccccgat gccgtccgtg caatggtaaa atctctaaag acagttggaa
     1921 tcaagatggt cgcaataggt gttggccgcg acacatttgg agccacgcaa attgcaaaca
     1981 atttgaagta cctggaatat gagaggacgt tggcagtatc aaggctaaag gacattccaa
     2041 atcgggtcct tgcggttttg cagacaagct agaatctgca agtatgtttt ggtacaatta
     2101 tcaaattcag ttttgataat tggcgaattt gatattaatc aagccttgtt agttttacca
     2161 atggcaaaca aaatgcaaaa aattttggtt cccttagacg gatcacaaaa ttctacacga
     2221 gcgctagaaa aagcaattac cattgcacaa ccaacagggg ccacaatcac tggattgtat
     2281 gttattcaca tacctgcaag atctgcaata cgctttaccc cgcaacaaag aaaaaaagag
     2341 atttcctttg ccgagtctat aattagcgag gctgcagaaa aggtggaaag ggctcaagta
     2401 aactttaaac caagaactga aacgggcgat cctggacaaa aaattgcgca agttgcaaaa
     2461 gacagcatct atgatttggt cgtaattggc tcaaggggca gaggtacagg aaaagaaatg
     2521 tttcttggaa gtgtatcaaa ctatgtgctc cacaagacaa acattcctgt gatggttgta
     2581 aaatagatca caatttttta tttttccaat gttttgcgga ctagctgatc ttatggaaaa
     2641 ttaatttttc cgcctatttt tgattctatt tctgcttttt cttgtgcgat gacatcaagc
     2701 attttttgct cagagtcgtc tagctttttg gcgttttcat aagagatttt ctttacattt
     2761 gactctgaat ccgtaaaggg taatcccaca agagatgcga caaagacaat aaggccaatc
     2821 ccaactactg tgcaaatctt tgccttaact gtatactgca tttgatattg tgcccaaggc
     2881 aaacaaaatt aattgcttgg tgatcatttc aaacaaatat tcatttttta attaaattag
     2941 agccccttac agccaaaaac acgttgtggc tattttatct gcgcattcac tttgatgata
     3001 ttggacatgc tgtatactgc aggacacctt tcctctgcca tcttgactag ttcttgtagc
     3061 ttttgcctgt ctgcgttttc tgcttgcgcg tcgatttgaa agttgattcc ctctgtgatt
     3121 ggctcatctg atacatcaaa tgtcttggca aaatttacgc cgcactcggt attgacactt
     3181 agttttgtca gcctaattcc ctgagacgct gcaatcccaa caaaggttcc aacaaagcat
     3241 gacgtaattc cggcaacaca gtaggccatc ggaccaagcc tgtttccacc ccctccaaga
     3301 aaagagggcg agtcgatttc tattagctgc tttccttttt caaacgcaag ctcggttctg
     3361 aactggtatc cctttgaggg atcaaaattc cactccccct gcagttttac tggctttttt
     3421 agagttgtcc tgtccttttt tccgccttct attgtttgcg aaaccttgtc taaatccacg
     3481 ttgttgatca cattcatgga ttggtattga tcttcgtaga ctaaatttgt tttttagata
     3541 ttctgacttg gggccttgat cggctccagt aatgctcaaa aatatcattg caccactccc
     3601 taaaggaatc gtcctcgctc aggaacatag agttcatgtc tatctggcct ttcttgtttg
     3661 gaaacatcac tgcgcaagag tcgtccgtta tgtagactgc aacatcaacg ctgtcgattt
     3721 gcctttgcac aaagttttcc ttggagctta gcttgtccag attttttatg attgtgtcta
     3781 ttatttcgtc aggaaaaatt gtgtttttcc caatcaatgt gtggacaatt acatcgtttt
     3841 ttgtcaggtt gatcagtatc ttgccttcag cagaccaggc ctgtgagacc attattttga
     3901 gcgctttttt tgtgttggat tcgatttttt ttagcttctc aaggacttcg gtcacgctgg
     3961 taattagctg gctgttttgt agcgccccta ttcgctgcac aaacttttct ggcatgtttt
     4021 gtatgatgtg atcctcaaag aattgtttgt acctgtacaa aaattcaaag tactggattt
     4081 gttttgccat cagcctgccg tattctgtta gtgaaagcgt tgcgtcagca ttcttttgta
     4141 ttattgaaga gcctgcaagc ttgtctgcat ttcggtgcgc gtcctgaatt gtaaggtcta
     4201 gcttttttgc cagctggttt aaccgcaatg gttctttgct caatgcgaca agtattgcac
     4261 accttgtttc acttgcaagg tctgtaaaca gctcaaaagg aagagattcc tctttcaata
     4321 atgaaatgat gcgatcgtcg tattaatact taaaaaaaag aaaaaagatt atggtaccat
     4381 ggttatggtg ccttgggttg caggccacaa ctggtcgatg aatccgtaga ctccgggctc
     4441 tgtaattttg attgtcgccg agccacctct gctcagaact cccgtgtcaa agacgccgtt
     4501 tggtatgccg ctttgcgcgt caactgagac tatctggtgt acccttccaa ctccgttgaa
     4561 tccgtcaacg ttgaagaatt ttacttcctc gcctgctgtg atggtgagct gttttggtcc
     4621 aaactgcggg tcttcgtttc ctgcaactat tactgtcctt ggctgcaaga ctggtgcaac
     4681 atcagatgcc gcctttgcct gtggctcggc attctcagac attactggta ctgacaagag
     4741 ggcaacaagt gccgcaactc ctgcaagcgc aaggatgctt ttgtgatttg ttttcatgat
     4801 gtgtttacgg cacaaattgc gatatagtta gctcaacaaa aaaatcaaaa ataaacaaaa
     4861 ttgtttattt tcatcatttg tgtttagctg atcttatacc atattcttgg tatgaaataa
     4921 tatgaaaaac aaaataattc acagcagcga acaccgcaca ctagagcacg actggctgag
     4981 gacaactcac cacttttcct ttggcgaata ttacaaccca gataggatga gctttggtcc
     5041 gctccgagtc ttcaacgacg atgtgataaa gcccggggca ggatttgact ttcaccagca
     5101 ccgcgacatg gaaattgtca cgtatgtcat agacggcgca cttgagcaca aggacaactt
     5161 tggaaaccat gacgtaatcg gttcagggga gatccagagg atgagtgctg gtactggggt
     5221 gtatcactca gaatacaacc attcacaaaa aggacctctc agattattgc agatgtgggt
     5281 ttttgcagac cataaggacc tcaagccatc ctgggagcag caaaaattca caaaacacga
     5341 aaggcagaac aagcttttag cagtaatcag cccccacaaa tccggcaaaa aaacactttc
     5401 aatacaccaa gacgcatcat tttacatctc aaacataacc caagaccata cagtagatca
     5461 cacgcttgcg caaaaaaggc aggcgtatct gtacgtcata aagggaacag tcaaagtcaa
     5521 cggccaatca ctaaaccaag gcgattctgc agaaatccaa gactcggata tttccatgac
     5581 ggcagcaaat gactctgaga taatcctaat tgatctgcca caacaatacg taaaaaactc
     5641 cgaaaccagt tagcagaaac taaaagacta gtccctaact tttttctata ttatttatac
     5701 tgataatttc ttagtgcgtc tttaatacca tgaaaaaatt tgcttaatca agtgtggatt
     5761 ggggcgtttc cataccggtg cctcagcaac cacaccccct atctcgtcct caaaatgtgt
     5821 agaacgcttt aaagagcgtc acatgagaat attacttgaa agtttggtaa aaacagaatt
     5881 taatcaatgc ttatttccca ttcatgcagt atctttgata tgacaaaata tttggtcata
     5941 cccatagtgg ttttgatttt tatcagttat tccattaatg gtgtttctgc ccaagagatt
     6001 ctcagtccac gcgagcagac caaaatggga gtagacccaa acaacgtcca gtgtcatgac
     6061 ggccttcagc tagtattaag atcaaatgtt atgcctgcat gtgtaaggcc agccacagca
     6121 gagatcctaa accagagagg tttgatactt ttcatggaac tggtgccagc accaattgag
     6181 ccgccgccaa ctgaaaaacc agaaccaagc ggcaaagaag cctcagacat aatagaggca
     6241 agcaacgagt tcatgcttga tttttactcg ctaaataacg caggacagga caacattttc
     6301 ttctcgcctt ggagcatcct tagcgcattt tccgtagtgt acgagggtgc caggggccag
     6361 actgcagaag aggtcgcctc tgcaacatac ctgccaaggg atgactctga tagaagagac
     6421 tcctttaaga caatccaaca gaatctcaac ccaaacagta cagaatacga gctgacaaac
     6481 gcaaacgcat tgtgggtaaa aacaggattt ggtgtaaaac aagaatttgt cgatattgca
     6541 aaacaatact atgacagtga ggtagctgaa gtagagtttc cagcaggcga gtcggagata
     6601 gactcgtggg ttgaaaatca gacaaatggc aagataaagg atctggtaaa gggcaagacc
     6661 aacgatatga ctaggcttgt aatcacaaac gcagtctact ttatgggaac atggaaggac
     6721 cagtttaatg caaacctgac aagccaagac gactttacac ttgactctgg tgatactgtg
     6781 gaggtgccaa tgatgtacca aaaatcaaga tttgattatg cagaaaatga caatctgcag
     6841 attctttcta tgccatacaa gggcgacaga ctctcaatgt tagtgctgct cccaaaaaat
     6901 aatcttggct cacttgagga ctcactgacc ctagaagagc tgagcagctg gcaagaacag
     6961 atgcaaaaca aagaggtgta cgtgtacatg ccaaagttca agctggaagc agagtatgat
     7021 cttagtcccc aaatgaaaga gcttggagta gagcttgcat ttgacccaga ctatgcagac
     7081 cttagcggaa ttgccgacgt aaagccaaac aacttgtaca tcggctttgc cacccacaag
     7141 gcgttcgttg acgtaaacga ggagggaacc gaagctgctg ctgcaacagc aattggaata
     7201 ggggtcacat ccgcacctgt agatcctacg gttttcagag cggatcatcc ctttgtgttt
     7261 ttgattcagg ataacgagac cggactagtt ctgttcatgg gccgagtttc cgacccatcc
     7321 tagatcaaaa attctaaatt tggctgcaag gttctggaat cctgtaaatg gattctatag
     7381 agatcacacc agaggaacta tcacaaatta tcgataacaa aattgccgcc ctgattttgg
     7441 acatccgcgc aaaggaaaaa tacatggacg ggcacgtgcc cggtgctgca aacgcagtgt
     7501 gccagaacat gcagcaaaaa caggtaataa tgtcaaagat cccgccatcg atgaaaataa
     7561 ttctaataga tgaggacgga acagagtcaa agcaaaacgc gattatgatg gccagattcg
     7621 ggtttgacgc gcactatcta aaggatggaa taaaatcctg gaataaggaa ctagtcaaaa
     7681 gctcatggga tgtgacaatt tctggcgata atctgtggga ttccctaaag caagacaagg
     7741 atgtcttttt gctagatgtt cgagagccag aagagtttgc agactttaag attccagggg
     7801 ctgtcaacat tccgctatca gagctgttca gcccggcagt agaatcgtca ataccaaaag
     7861 acaaaaaaat tgttacaata tgctcgcatg gaaaccgttc catggttgca acgtttgcct
     7921 tggcgcaaaa aggaatcgag gcaagctcgc tagtcggcgg gatgtctttg tggaaccagg
     7981 tcctaaacgc cactacacta aaggaaaatg atcttacagt aatccaagta gaaaaggtgg
     8041 gcaaggggtg cctctcccat atcgtcggct ctggtggcga ggcagcagta attgatccta
     8101 catatcctcc atcaaaatat gtcgagtttg cacaatcaga gggactcaaa attacaaagg
     8161 taatcgatac gcaccagcac gcagaccatg tctctgctgc aaaagacttg gctcagcttg
     8221 caggcgcaaa actatacctg agtaaattag aagaatacaa aatagattcg gaaaggattg
     8281 gcaatggcga ttccatacca tttggctcaa agcaactaaa agtgattcac acacccggcc
     8341 acacagcagg aagcatgtca tatgtagtag atgacaggta cgtcttttct ggcgacacgt
     8401 tatttgttga aggggttggc aggcctgacc ttcgcgacca agtgcaagaa tatgcaacaa
     8461 gcctttacga caccctgcac aacaagatac tcaaattctc agactatacc aaggtgtttc
     8521 caacacacca ctcagaggga atcaagccca ctgaaaacgg aatttattac acaacagtca
     8581 aaatggcaaa gagtctcccg ttgctggatt tatcgcagcc ggattttgtg gccaaagtta
     8641 tatcaattac aacgccacgc ccgatgaact attccatgat aattaaggta aacaagggta
     8701 ccataccaat atctccaatg cagattccgg acctcgagat gggcccaaac cgctgctcca
     8761 tccggatgta gcttgactgc attcaagtgt ctctcagtct cccagccttt tgcagacctc
     8821 atagtcaaag gaaagaaaac aatcgagtta cgatcgtgga ataccagcta tcgcggcgag
     8881 ttcctagtcc atgcgccaat taagattcgg acatctgatt gcaaaaggct tggaattgat
     8941 caggcctcat tgccagttgg agccataatc ggcaaagcta ccatacatga tgtaaaatta
     9001 taccaaacca gaaaggaatg gcaggctgac caaaaatatc attttgcaga ctgcgatttt
     9061 tctggctcta gatatggatt tttgctaaag ggcgcaaaat catttgtcat tccaattcca
     9121 tgtaaaggaa agcttgggtt ctttgatgtg cagctagatt ctaccaaggt aaaggacgcc
     9181 gacatcacat cagaaatatt tgatgaggaa taccgcacgc agtggattaa ccaccattag
     9241 gatgatactt tgacgccgtt ccaaaacgcc acatggtttt ttattttctt tgcagcatcg
     9301 tttggttcag gatagtacca ggcacagtcc ttgttggtct tgccattaat caccactgaa
     9361 aaataatttg ccataccctt ccagccacaa attgtagttg tgtcagattt tacaaaaaat
     9421 tcctttttta ttgaatcgta tggaaagtag tggttcccct caactattat ggtctggtca
     9481 ctttctgcta gtatggtatc gttccacgta gctttcattt tctggaatta tgtaatcacc
     9541 gaataaacat ttttcgcagg gtgcggaagc caagtttttg catctagttt accgattcac
     9601 ttttattgtc atcccagggc agcgtcacta tgaaactctt taggagaaac agcacgcagg
     9661 atatgagtcc tgtctgcaag atttgcggaa tggagtttac cgaagtggaa cgaatgatgc
     9721 gccacatgac aaaggcacat tcaaagccat gcaagggcgg aagctgcggc tgcagctaag
     9781 actgattctt atacagaaaa caagaccagt gcaaaatagt ttttctcatc gcgccagatt
     9841 ttaaccgggc ttagccctgc tttttttgcc atttttctaa tctgcgatat ggcgtacttg
     9901 tgggaatatt ccgttctgat agtctcgcct ttttccaaat ccacggaaag tccaatgtcc
     9961 tgtatggata cttggtgttt ggtctttgag cggatgtgca tttctattct tttttctttg
    10021 gaattgtaga atgcaacgtg ctcaaatttg gcatagtcaa gatcgcctct cagctcgtag
    10081 tttattcgcc tcagcaggtt cagattaaag tcccgcgtaa cgccctccga gtcgttgtat
    10141 gcagcctcta ggactttttt gccctttacc agatccacac caagcaagaa caggtcgcct
    10201 gaattcatgc tgcttctaac ctttcttaga aagtcttgcg cactgctctg atcaaagttt
    10261 ccaagactgg agccaaaaaa cgcgattatt ttttttccgg ccacgtcctt gaccaaatcc
    10321 agtccgcttt cgtactggtc cacaatccca gttatttgca gattatcaaa ctcgccttgc
    10381 agcctctggc tgctctcctt tactacattt gagatatcaa ttggatagta tgctattttt
    10441 ttctggcttt tgcacaatac ctcaaagagg tgtctcgtct ttactgcaga gccgcttccc
    10501 aattccacta cagaataatt cccatctagg tattcagata gttcctgctt tgctgaatcc
    10561 agaatctcca gctccgctct ggtcagatag tattccggga gatcgcatat cttatcaaat
    10621 aattcggagc cggcctcgtc gtagaaatat tttgatggga caaatttttc ctttctggta
    10681 aggccatacg ccacatcctt tgcaaagtct tgctcttttt tttctagaga ttttatcaga
    10741 gtgcagtttg gagagaggac tgtccttaca taattagagt cggaatgagg cttcattttt
    10801 cgttgaacca attaatatgg agctcccttt attcgttgat tcttaaaagc ttcgatggaa
    10861 tcataaacag agaatcattt gagtaatgtc atttgcgttt tttatgattg aaaaaaagaa
    10921 acaaatgaat actaaaaaac cactacacat agaaatgctg tctacatatt acctagtaga
    10981 agagttcaag gcaacccgct caaccacact aagactggtc gacacactag aaggcgacga
    11041 ttttgcagta cagacagatg actatgtcag cccgccaaaa tggcacctag gacatgtgag
    11101 ctggctattt gagatgctgc tccagcaggc aagcaaggat tacagggttt attctacaga
    11161 gtttctaaag tacctaaact cgtactataa caagtttgga gagcagcacg aaaaggcgca
    11221 aagaggctca gttgcaaggc caactactgg tcagatccta gaatactatg acattatcag
    11281 taaacaggta atcaattttc tagagacaaa tccggcaaat gattcgcaaa gaaaattatt
    11341 tgagcttgga atacaccacg agtgccagca ccaggagtta ctggtgtacg atattcagca
    11401 catacttgga gacaagtaca ggccgtccat aaaaaaacag gccccaaaac cacatgcaaa
    11461 gcaaaaacag atccacgtag aaggtggcct gtataatctt ggatattccg gaaaagattt
    11521 ctgttatgat atagaactac cagagcacaa agtctaccta aacgactaca aaatcgatgc
    11581 acttcctgtg agttgtggtc agttcatcga gttcataaat tccggcggat atcaggatta
    11641 tcggtactgg ctgtctgatg gatgggatgc cgtacagaaa aacaaatggg agtcccccat
    11701 gtactggtat aagatagatg gtgattggta tgttgatgat tttcttggga gacgtaaaat
    11761 taatccaaat gagccggttt gtcatgtcag tttttacgaa gccgacgcgt actgcaggtg
    11821 ggcaggcaga cgccttccaa cagaatctga atgggaaaag gcagcttgct ggaatgagga
    11881 aaaacagatc aaaacaatat ttccttgggg taatgaatca ccatctgaaa aacatgcaaa
    11941 tcttctggaa tcatttctgt ggggatgctc cgagattggc tcatacccag atggcaaaag
    12001 ccattatggc tgccaccaga tgattggcga tgtgtgggag tggacatcgt ccgaattcgt
    12061 aggatatccg ggattcaagt caggcttttc tgaatacaac gacaagtggt ttacaaacca
    12121 aaaggttctg cgcggcggct cctttgccac acctcaaata tcaatccgag gaagctatag
    12181 gaactttttc agactggatg aaagatggtt gttttctgga tttagatgcg ttgaaaatat
    12241 tcgaatttag aaaattcagt ccgtgactga tcaacaatca aggcacaaat gcttttagtg
    12301 tgaaaaaact aatttcatta aatacatgat atagtttaac tctttttaaa aaaataaaaa
    12361 aagacagagt ccccattgga agggattaat gaatggacag ttcactatat gatcattttc
    12421 tattggtcgt gcatgagagg aattgtgagc gcatcctaac ttttttagct agccaaacaa
    12481 tggtcagatt tccatatctt cctactgtat gtgtgtactc atactgccaa aggcttagaa
    12541 caaattttta ataccctagt ttttcatagg taatcggact tggtactgtc gaattttgtc
    12601 tagtggtgtt agtggagatg acgacgaagt tcttgtccgc tacaagttca taggcacgca
    12661 caaggcatca gaaatccgct gcacgtacct ccaatacagg aatcttaaga tgcttccaaa
    12721 catcgagttc tgcaccatag taaaagatga agatgactct gagaaaaatc catcttgatt
    12781 tctatactgg cagcaacaga agcagaattg gttagtttag tgggccaacc gttgtgtaat
    12841 ctatcaaaac catcaggagc ggatgaactt tgagacagct atgattgaat ccttgatgat
    12901 gtctttttcc tcctgagaca ttaccggctt gtccaaaagc atgacaattt tggagaggat
    12961 gcttgctatg ttttttgcag ccttactttt tgtctttttt gattttgcct ttctcttggt
    13021 tccttttttt gatctcttgg ttcgtgtatg ccttctggtc ttcttttttg ctggcctctt
    13081 ttttgctggt ttttttgctc gcgttagatt tcttgccgtc cttgaggatc tgcgtttgtt
    13141 ctttctcttg gctttggatc ttctggattt ggccacgggg ggagctggtg cttggaagga
    13201 taaaaaaagc taaaatcaca tctttttagc taagattgtt tttaatctca agtgaaaact
    13261 tgcatagtta ttcatcacac gcaggcaaag ctcattgaac cgaacgcttt ttacaaaatc
    13321 caagtcaaac catgacggtt ggcaaaaact aggagtaaaa agcttgcaaa aaaaaggatc
    13381 agatacgcat caaaggcgcc ttatagatta aaggagattc caataaagca gatccgagtc
    13441 tggaaagagg cacaggcaag aaagcttgac agggaaggaa tttcagagct ggcaagatca
    13501 attaagaatg agggactgca gaatccccca atgctgcaaa aagaaggaaa gaacacgtat
    13561 cttttgatgt cagggcagcg aagactggca gcactcaaaa gacttggcgc aaaaaagatt
    13621 cccgcactgg tcctgaccaa aaaaacagag tatgaggtac ccgatgccaa ggcagcatct
    13681 gtcattgaaa acattcaccg caaaaacatg aatgtaaagg acatggctgc cgcgtgtgca
    13741 tttttggcag atcagatggg caagtcaaag gcagcgcagt cattaggact ctcaatgtcc
    13801 acattcaaga aataccatgg gtttgcagga gtcccagaca agctaaagga acttgtacca
    13861 aggatcatat ccagagatga tgctacaaaa ttataccaga tcattccaaa tgtttcaaaa
    13921 tctgtgaaga ttgcccacaa aatatccggt cttgatactg ccacaaaaaa acggtacctc
    13981 aaggttttgg tgcgcaatcc gcattcacca cacaagaaaa ttttgaaaaa ggccagatca
    14041 atgacagttc agcaaaaaat ttcactaagg ttgtcaaaaa gacaggcccg tgggttgggt
    14101 acacagtcac gccatcaaga cgtagaccca aatgagcttg caggcaagat catatccgac
    14161 tggcttggca gaaagggtta ctgaatagct ggcccaaaag gcctcaaaga ctgagggctt
    14221 gattcaagat tgggaagaag gcgctcttgg agggcagatc atcttattca tcactaaatc
    14281 tatgtcccaa aaataggaca tggctaggct gaaagtattg cagagtggat taaccgtcat
    14341 taaatccatg cgtgtgtgtc ctaaaaacaa aatagcagat ttatatggaa gatttttctt
    14401 ccatatgtaa gaaaaatctg acacatatga aactacacaa atatctagag ggagtgcttg
    14461 gaaacaaggg agcaatcagc gtgcttcgtg ccctagtcaa ccacaggggc agaacctaca
    14521 ccatcagaag actggcaaaa gacgctggaa tttcacacac agagacgtca gcaaccatac
    14581 aagacctaga aaagctcggc atagtccaag tccaaccaat tggaaaggca tacaacatat
    14641 ctctaaacga gaaaagctac gtgctggcaa acatcataga gccagttctt gacgtagaga
    14701 aaaaatcgct tgaccaagta atttcgatct gcaaaaaaca ccttagctca aagaaaatca
    14761 tctcagctgt aatttttgga agtgtggcaa gaggcgaaga aaaggaggac agcgacattg
    14821 atcttctgat catatcaaat gactttgaca aagcaattga aatcgtttca agactttcag
    14881 aagaaatgtc attggcattt cataccagga tctctcacat aacattctcc gaaaaaaaac
    14941 tcagatcaaa aagaaacagc gatctgatac gctcaattat taaagagcac attctggttt
    15001 ctggaaaaga attggaagat ttaatcaaat gacaaaacca gtcgatccaa aaaaatcacg
    15061 caactatatc ataaaagcag agaattcgct tcgcatagca aaaattgcaa tagagcataa
    15121 cgcatacgat aatgcagtaa tgactgcaat acatagtgcg ataaacgcac tagacgccct
    15181 tacttcaagt gaaaaaggat tagggcctca ggtgaacacg atgaggttct cacacttctc
    15241 aagggcattc tgacgccaaa cgaatatgcc gatattgaaa aacagttttc ttctttgata
    15301 gatctcaaaa atactgcaga ataccagccg gattttacga gtcccgagga ggctagaaat
    15361 tcaataaaac gggcgcagag aatcctcgac aaagtcaagg ccaagatgaa agccaagaat
    15421 ggataaatcc tcacaaaacc agaaaacccc gttctaccat caccttatat aaagggggta
    15481 tataaaaaag cacgaatgcc gcagacaaag ccgataatat ctgtagaaaa tgtagtagcc
    15541 tctgcatcag ttgatcagaa aattgatctt aacgaggtga caaaaaagtt tccagacacg
    15601 gaatatcatc cagaccagtt tcctggattg gtctttaggc taaagactcc gcgcactgct
    15661 actttgattt ttaggaccgg caagatggtc tgcactggag caaaatcaga agagatggca
    15721 ataaaggctg tccagactgt tgtggaccga ttgcgcaaag gcggaatcaa gattaaaaag
    15781 gatgcagaaa ttgtagtgca aaatattgta gctgcaataa atctgggcgg caagattcac
    15841 ctagagcatg ccgcacgaac tcttcctcgt agtatgtacg agcccgagca gtttcctggc
    15901 ctaattcata ggatgcttga cccaaagact gtgatattgt tgtttgcgtc cggcaaattg
    15961 gtttgcactg gagcaaaaaa ggaagctgat gtttatagat ctgttaacaa cctgcattct
    16021 ttgttagaag aaaaagggtt aatgatttac gaccagtaga atttttgtaa attttataat
    16081 atttagctga atcctgcacc aaatttgtct gcttgttgta gtgggtcata gtcgcctgca
    16141 ccttttagct ttaggtagag gaatgtttcg ttga
//
LOCUS       DASWKH010000102        10763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1365085, whole genome shotgun sequence.
ACCESSION   DASWKH010000102 DASWKH010000000
VERSION     DASWKH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365085"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGA92_07795"
     CDS             complement(<1..195)
                     /locus_tag="VGA92_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846346.1"
                     /translation="MLRCRGNCKTKYQEFLGQISLKYNGQKRCGVCDIYLRWDGAKCP
                     CCSSVLHVRPRHSRAKIKYYE"
     gene            complement(240..806)
                     /locus_tag="VGA92_07800"
     CDS             complement(240..806)
                     /locus_tag="VGA92_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846347.1"
                     /translation="MLLHFIFLVTQDDLQNRSKEFEYIQQMAQFYKKWIKDTFSTDVD
                     IQSDQMITPKSGLLHRIDTSALLEDHRKRGQGIFHFYLCNFRPLWTDCTCEGYSAENF
                     GMSLWQRPKDQNDALFLAEKNCTVVSHELSHELLRQKKTKNQAELVHDVWTKHLFDEL
                     PFEQYGKDFEPTTNDPYFFTIDASSFRL"
     gene            complement(825..1064)
                     /locus_tag="VGA92_07805"
     CDS             complement(825..1064)
                     /locus_tag="VGA92_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9846348.1"
                     /translation="MATAYVLINCELGSEEAIIKQLKSLDGVREVHGTFGAYDILAKI
                     ESPTVEALRETITWKIRKIEKIRSTLTLMGIEGQS"
     gene            complement(1112..1522)
                     /locus_tag="VGA92_07810"
     CDS             complement(1112..1522)
                     /locus_tag="VGA92_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9846349.1"
                     /translation="MVNYWLCKQEPSTYNIDKLENEKKTIWDGVHNNLALKHIRQMKK
                     GDFAFFYHSGDEKQIAGIMEIASGPRPNPKEKDPRFVVVDVKFKKRLDRPVTLGEIKA
                     NNKFKDWELLRISRLSVMPVSSQIWNEIIKISQK"
     gene            complement(1860..2552)
                     /locus_tag="VGA92_07815"
     CDS             complement(1860..2552)
                     /locus_tag="VGA92_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846350.1"
                     /translation="MTKLNTKYTQALALLLVAALAPASIAFAQSSTEAETTTETTENT
                     ANSDVKIKTRADVKARVSDILEKKMDLIKDEVKTRYVVSDTLADRGTDLTFRGHTDGW
                     AIVGGKAHMSSIAFGGDAHHIGGGNWKITSAGELTVGDRHAKLDLSGFARGNIIHLQG
                     SGSLDNGEAFRILLRGHFAPTDDRNVYAIAFTNAHIQYISSGLRVPLMQVGSVTIIDT
                     ILPATVEPVPAQ"
     gene            complement(2646..4280)
                     /gene="pheT"
                     /locus_tag="VGA92_07820"
     CDS             complement(2646..4280)
                     /gene="pheT"
                     /locus_tag="VGA92_07820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9846351.1"
                     /translation="MPVVTLYFNRLEKLVGKKANKNKILDTLPFLGLDIEEQTNEYVR
                     VEYSPNRPDYATDVGIAAGLQGLLGIKKGIDKLTFKKEKSFLIRTDPSVKKIRPYVSG
                     IAAKNGKLDDEAIRQLIALQEDLHFGVGRRRKKASIGIHNLDAVSFPLRYTTVPKDHK
                     FVPLASTQEMTAEQILAKTDVGSQYGKVLGGATRLPMILDANGNTLSFPPIINSVLTA
                     VSTSTTNLLVEVTGTDKNAVNDTLSVVATTLQNFGFTLFDLKTDAKSSAESLKTRAMA
                     LEPTLVNQTLGVSLTNQEICNSIKKCRLDAVVKAKKIICTIPRFRFDVLGPMDLIEEV
                     ALGYGIQKLKPTIPLSGAVGQKNELTKRLDRLGQIMVGLGFTEALNTSLTSRHTLYEQ
                     TNRNSSGIIEVAESKSLEHTVLRDSILPGLLENLSRNIHESYPQKLFESGTIFSNANP
                     IDESISLACISAHKDANFTEIKAVLQSLLKTDSDIECKTKTSTNPIFATGNTADILVG
                     DKKIGVIGEIDAQVIDNFKIRMPVCGFEITLSDFIL"
     gene            complement(4271..5677)
                     /locus_tag="VGA92_07825"
     CDS             complement(4271..5677)
                     /locus_tag="VGA92_07825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9846352.1"
                     /translation="MSQVLHPIEKTIIKLLKTTSNLTDEQISEKTNLSIDQTRRGIEW
                     LRLKNLAVVLESEKSFVRLGKNGLLARQEGLPERKLVDLIASKPLAFGELQKKLGAET
                     NIAIAIAKKNDWIEMQKGESINIVSLKQKPDQTSEEKLIAFISENKIPLEQIQDSAAL
                     ESLKKRPDYIVIETTKSRTVSLSEQAKSIDVEKEDDGAIDVEADVPRVFAARTHPLKD
                     VINEIREVFVSLGFTEVMGNFAQPSFWNFDALFTPQDHPAREMQDTFYIKEKLADKFA
                     SQNQINQVSKAHKKGWKYDWSVDVAKKMVLRTHTTCVTIKYLAENKPQEARIFSLGRV
                     FRNEKVSYKHLVEFHQVEGIVVGKNVSLRDLMGIQTEFYKKMGLDKIKFWPTFFPYTE
                     PSLQTMVYNQRLDKWVELFGMGIFRPEVTRPFGITRPVLAWGGGIERIAMLKYGLDDV
                     REFYNNNLSWLRGVPKCL"
     gene            5730..6839
                     /locus_tag="VGA92_07830"
     CDS             5730..6839
                     /locus_tag="VGA92_07830"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9846353.1"
                     /translation="MPSDEFIVTPWHVEGEIDYDKLIKQFGTEKITGELLKKIEKVTG
                     EIHFMLRRGIFFSHRDLGRLLADYEKGKKFFLYTGRGPSGHTHIGHLVPWVFAKWLQE
                     KFDTTMYFQLTDDEKFYAKQDLTMEQTKKFAYENALDFIALGFKPDKTKIIIDSQNIK
                     TLYPLAVQVAKKINFSNTKAVFGFTNETNVGMIFYTSLQSAPCFIEDRPVLIPLGVDQ
                     DPHFRITRDVAPRIGRPKPALIHNIMIPSLTGPRGKMSASEESGTIYTTDSPEMIKKK
                     INKYAFSGGQPTVEEHRKLGGNPDIDVSYQYLRIFFEPDDKKLKQIHDDYKSGKLLTG
                     ELKAILIEKVTEFLKSHQQKREKAKGQLEKFLFED"
     gene            6840..7121
                     /locus_tag="VGA92_07835"
     CDS             6840..7121
                     /locus_tag="VGA92_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846354.1"
                     /translation="MKIKIACQDKHEASKLASLVFIKDGKETFISSILNIYDNEVVIA
                     LKDKSAHSIVLKDDYQVELFADFIQSVLDGISQIIDAKAENDTVEITKG"
     gene            complement(7118..7447)
                     /locus_tag="VGA92_07840"
     CDS             complement(7118..7447)
                     /locus_tag="VGA92_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846355.1"
                     /translation="MSIINSMMKKFTKNVTGLQEGLHPENLSFWYSTIIAEAKEMAPP
                     WLADKIKVKQDPILPMKFNLDISKRAVRYFMMAVDNNSIKMPNSTRLYFLKVQETLSD
                     EMDKSLV"
     gene            complement(7511..7975)
                     /locus_tag="VGA92_07845"
     CDS             complement(7511..7975)
                     /locus_tag="VGA92_07845"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9846356.1"
                     /translation="MTRFDVVAMGGTFDIIHKGHIALLEAAFSASKKVIIGLTSDELA
                     KKKGKNLNHPYLQRLDTLKQFIEKNFQNKEYLISKLDNDFGPAAIEGNVQALIVSEET
                     AHKGKTLNKLREEKNLGPVETVVVPMALAKDGQRISTTRIRNSEIDAQGNLL"
     gene            complement(8005..9084)
                     /locus_tag="VGA92_07850"
     CDS             complement(8005..9084)
                     /locus_tag="VGA92_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX9846357.1"
                     /translation="MKKILVISIFAFLLLPVSQGFGQVTDTPSTLVVKLTNTTPFSYK
                     DDQGYTIVIGEVENTTTFPVTNIKVWVGFYSGKTSGSGEAPLETVTGSTLLDVIPARG
                     KSPFMIKSKTANPEISKVTINILGFNPATQKQQMLEITPDTLAIGNTIKLSAEIKNNG
                     AQESTKTNVYLIAFDAFKPPRIVGIQTLTIDEITQGQSVNIEFDALMDYRATTFKVLA
                     ESDNYQSPITDVTDISLDTLTRLISINDVGVMDLSGKRVSQLKVGEPVDIASDLSIQY
                     FALSGSKQPYVYYAQVKQFGETAPVEFLGIAEGEFSSAVPQTASVNWTPEREGGYFIE
                     AYVWDPNGVALAAPSKTISIVLVTS"
     gene            complement(9392..9463)
                     /locus_tag="VGA92_07855"
     tRNA            complement(9392..9463)
                     /locus_tag="VGA92_07855"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9429..9431),aa:Ala,seq:tgc)
     gene            9550..10101
                     /locus_tag="VGA92_07860"
     CDS             9550..10101
                     /locus_tag="VGA92_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9846358.1"
                     /translation="MVVMESQVQLKKGDTAPDFELLGVDDKKHSLASYGNYDAVLVIF
                     ICNHCPYVKAKVDAINEVATKFKDKVAVIGINSNDSVAYPDDSFENMKKFAAEKKIAF
                     SYLVDDAQQVAKRYGAVCTPDPFLFDKQRKLAFHGRIDNAMKPEDVATEKTMIANIER
                     LLSGQPIPKDFDPSIGCSIKWKS"
     gene            10142..10235
                     /locus_tag="VGA92_07865"
     tRNA            join(10142..10180,10197..10235)
                     /locus_tag="VGA92_07865"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10177..10179,aa:Ile,seq:gat)
     gene            complement(10255..>10763)
                     /locus_tag="VGA92_07870"
     CDS             complement(10255..>10763)
                     /locus_tag="VGA92_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX9846359.1"
                     /translation="SMDVNPIDAVFNTISASTNTGFQTINLQDLSPIPGIVFIIIMII
                     GGCGFSTAGGMKVFRLIKLVNVKNLILRKKLSHSDKNHIITASILVVLFPLIPYFVAV
                     HMESLGYDFEDSYFDAVSALTTTGLGAGTIGLDLDPTTLTLFSFLMIFGRLEIILLIY
                     MVVPRLIP"
BASE COUNT         2841 a         2262 c         2230 g         3430 t
ORIGIN      
        1 ttcataatac ttgatttttg ccctagagtg ccttggcctc acatgtaaaa ccgaactgca
       61 gcacgggcat ttggcaccgt cccaccttag gtaaatgtca cagacaccgc atcttttttg
      121 cccgttatac ttgagtgaga tttggcccaa gaactcttgg tattttgtct tgcagtttcc
      181 cctacacctt aacacaatac aatgctgttc tgcgaaaatt aaagaagctg gtaattgtat
      241 tataacctaa agctggacgc atcaatggta aaaaagtaag gatcatttgt tgtgggctca
      301 aaatcttttc cgtactgctc aaatggcaat tcgtcaaaga gatgttttgt ccagacatcg
      361 tgcactagtt ctgcctgatt ttttgtcttt ttttgccgca agagctcgtg cgagagctcg
      421 tgcgatacta cggtacagtt tttttctgcc aaaaagagcg catcattttg atcttttggt
      481 ctttgccaga gcgacatgcc aaaattttct gcgctatacc cctcgcatgt gcaatcagtc
      541 cacaacggcc taaagttgca caggtaaaaa tgaaaaattc cttgtcctcg ttttctgtga
      601 tcctccaaaa gcgcactggt gtctatccta tgtaaaagac ccgactttgg cgttatcatt
      661 tggtcggact gaatatcaac atctgttgaa aaggtatctt tgatccattt tttgtagaac
      721 tgcgccattt gttgtatgta ttcgaattct ttggatctat tctgtaaatc gtcttgggtt
      781 accaaaaata tgaagtgcag tagcatgaga agaaaaagaa gtaattaaga ttgaccctca
      841 attcccataa gggttagcgt agatcggatt ttttctatct tacgaatctt ccatgttatt
      901 gtctcgcgta gtgcctcaac cgttggagac tcgatcttgg ccaagatgtc gtatgcacca
      961 aaggtgccgt gaacttctct tacgccatcc agagacttta gctgtttgat gatggcttct
     1021 tcggatccaa gctcgcagtt tattaaaacg taagcggttg ccatgattaa cgtatacacg
     1081 tcgggatata tcaataaacc tattgccggt tctatttttg agaaattttg atgatttcgt
     1141 tccaaatttg agacgaaacc ggcatgacgg agagtcgcga tattcgcaat aattcccagt
     1201 ccttgaactt gttgttggcc tttatctcgc caagtgttac gggcctatca aggcgttttt
     1261 tgaatttgac atcaactact acgaatctcg gatccttttc ctttgggttt gggcgtggtc
     1321 ccgatgctat ttccattatt ccggcgattt gtttttcatc gcccgagtga taaaaaaacg
     1381 caaaatcgcc ttttttcatt tgccttatgt gctttagtgc caagttgttg tggacgccgt
     1441 cccagattgt ctttttttca ttttctaatt tgtcgatatt gtatgtgctt ggctcttgtt
     1501 tgcagagcca gtagtttacc atcggtatca catattttac aaatctaata tttgctttgt
     1561 tggcatttgt aagaatttgt accccggtac tgactcacat actatcacgg gttttagcct
     1621 aaatcctcaa gcatgctctg taaccggggt attgccccat gtcgcacacc tgggtggacc
     1681 cagaaatcat tttttagcat catattcatt ccagtccgtc tgtgtgcctt tttagggcac
     1741 gtaccatttg atcaagtcaa atattaattc aaagctttag tgttgtacag ttagtatagt
     1801 aattttgcgg caaaccagtc gtttgtttag ttattttgaa aaaataagaa agaagctgct
     1861 cactgagcag gtactggttc tactgttgct ggtagaattg tatcgatgat tgttacagag
     1921 cctacttgca ttagtggaac tctaaggccg ctgcttatgt actgaatgtg tgcattggta
     1981 aatgcgatcg cgtatacgtt tctgtcatct gtcggcgcaa agtggcctct caacagtatt
     2041 cgaaaggctt caccgttatc aagtgatcca cttccctgca gatgtatgat attgcctctt
     2101 gcaaatccgc taaggtctag ttttgcatgt ctgtcgccaa ctgtcagttc gcctgcggat
     2161 gtgattttcc agtttcctcc accgatgtgg tgagcgtctc ctccaaatgc aattgaagac
     2221 atgtgtgctt ttcctccaac tattgcccat ccgtctgtgt gtccacggaa tgtcagatct
     2281 gtgccacggt cagcaagtgt gtctgagacc acgtatcttg ttttaacttc atctttgatg
     2341 aggtccattt tcttttctaa tatatcagat actcttgcct tgacgtcagc tcgtgttttt
     2401 atttttacat ctgagttagc tgtgttttcg gtagtttcag ttgttgtttc tgcttcagtc
     2461 gaagattgcg caaatgcaat ggatgctgga gcaagtgcag caactaacaa tagtgccagt
     2521 gcttgtgtgt atttcgtatt gagttttgtc attagcagcg gttctgcttt tttcttttaa
     2581 ggagttacgc agctttgtta ctgatgagtt atcagtgagt cacatcagaa ttggaaatgt
     2641 tgtgcctata aaataaaatc agatagtgtt atttcaaatc cgcagacagg catgcgaatt
     2701 ttgaaattat caattacttg tgcgtctatt tctccgatta cgccaatttt tttatcgcct
     2761 actaaaatgt cagcagtatt cccagtagca aaaattggat ttgtcgacgt ttttgttttg
     2821 cactcaatgt cggaatctgt tttgagtaac gattgcagga ctgcctttat ttctgtaaag
     2881 ttggcgtctt tgtgtgcgct tatgcatgcc aagctgattg attcgtcaat tgggtttgca
     2941 tttgagaaaa tggttccact ctcaaataat ttctgtgggt atgattcgtg tatgtttctt
     3001 gagagatttt ccaacaatcc tggcagtatc gagtctcgca gaactgtatg ctctaggctt
     3061 tttgattcag ctacttcgat tatgccagag gagtttctgt tggtttgttc ataaagtgtg
     3121 tgtctgcttg tcagactggt gtttagcgcc tctgtaaatc caagccccac cataatttgt
     3181 ccaagcctgt ccagtctttt tgtgagctcg tttttttgtc ctactgcgcc agaaagtgga
     3241 attgttggct ttaatttttg aatgccgtac ccaagtgcga cttcttcgat taaatccatt
     3301 ggcccaagaa catcaaatcg gaatcttgga attgtgcaga tgattttttt cgcctttact
     3361 actgcatcca gtctgcattt ttttatcgag ttgcagattt cttggtttgt tagtgatact
     3421 cctagtgttt gatttaccag cgtcggctca agtgccatgg ctcttgtctt tagtgattct
     3481 gctgagctct ttgcgtctgt ctttaaatca aagagggtaa atccaaagtt ctgcaatgtt
     3541 gtcgcaacga cagaaagtgt gtcatttact gcgtttttgt cagtacctgt cacctccact
     3601 agcagattag ttgtgctcgt ggaaactgct gtaagtacgg aattaattat tggtggaaac
     3661 gacagtgtat tcccattggc atccaaaatc attggcagtc tggtagcacc acccaagact
     3721 ttgccatatt gcgagcctac gtctgttttg gcaagaattt gctctgcagt catttcttgt
     3781 gtgcttgcca gaggcacgaa cttgtgatcc tttggtacgg tagtgtatct aagtggaaac
     3841 gatacggcat caaggttatg aattccaatc gatgcctttt tgcgtcgcct acctactcca
     3901 aagtgcagat cctcttgtag tgcgatgagc tgcctgattg cctcatcatc tagtttaccg
     3961 ttttttgcag caatgccgct tacatacggg cggatttttt ttaccgaagg atcagttctg
     4021 atcagaaacg atttttcttt tttaaatgtt agcttgtcga ttcctttttt tattccaagc
     4081 agtccttgca gccccgcagc tattccaaca tctgttgcat agtctggcct gttggggctg
     4141 tactccactc ggacatactc atttgtttgc tcttctatgt caaggcccaa aaatggcaag
     4201 gtgtctaaga ttttgttttt gtttgccttt ttgccaacga gtttttccag tctgttaaag
     4261 tatagtgtca ctacaggcat tttggcacac ccctcaacca gctaaggttg ttgttgtaaa
     4321 actccctgac atcgtccagt ccgtacttga gcattgcaat tctttctata ccaccacccc
     4381 aggccaagac aggtcttgta attccaaatg gtcttgttac ttccggccta aagatcccca
     4441 tcccaaagag ttccacccac ttgtcaagcc tttggttgta gaccattgtc tgcagcgacg
     4501 gctccgtata tgggaaaaat gtaggccaga attttatttt gtctagtccc atctttttgt
     4561 aaaattccgt ctggattccc atcagatctc tcaatgagac attttttcca actacgattc
     4621 cctctacttg gtggaactct actaggtgct tgtagctgac cttttcattt ctaaaaacgc
     4681 ggccaagtga aaatattctt gcttcttgcg gcttgttttc ggccagatac ttgattgtaa
     4741 cgcatgtagt gtgtgttcgc aaaaccattt tctttgccac atcgacactc cagtcgtact
     4801 tccatccttt tttgtgggcc ttggatactt ggtttatctg gttttgtgat gcaaatttgt
     4861 ctgcgagttt ttcttttatg tagaaggtgt cctgcatctc tcttgccgga tgatcctgcg
     4921 gtgtgaacag tgcgtcaaaa ttccaaaagc ttggctgtgc aaaatttccc atcacctcgg
     4981 taaatcccaa gctgacaaag acttccctta tttcgtttat gacatctttt agtggatgtg
     5041 ttcttgcggc aaagacccgc gggacatcgg cctcaacatc aattgcgcca tcatcctcct
     5101 tttcgacatc gattgatttt gcctgctctg aaagcgagac tgtgcgcgac tttgttgttt
     5161 caattacgat atagtccggt cgttttttta gcgattctag tgcagcagag tcctgtattt
     5221 gttctagcgg aattttattt tccgaaataa atgcgattag tttttcttcg cttgtttgat
     5281 caggcttttg ttttagtgag acaatgttaa ttgattcccc tttttgcatt tcaatccagt
     5341 catttttctt tgcgattgct attgcaatgt ttgtttcagc gccaagtttt ttttgcagct
     5401 cgccaaacgc caaaggcttg cttgcaatca aatcaacgag ttttctctca ggcaatcctt
     5461 cctgtcttgc caaaagaccg tttttgccca gcctcacaaa tgatttttca gattccaata
     5521 cgactgcaag attcttgagt ctgagccatt ctattcctcg ccttgtctga tcaattgaga
     5581 gatttgtttt ttctgagatt tgttcatcgg ttagatttga cgttgtcttg agtagcttga
     5641 ttattgtttt ctcaattgga tgtagaactt gagacaacag caaaacgacc cgaaaagact
     5701 ttttaaacct taacctaact caaacttgaa tgccttctga tgaattcatt gtcacaccat
     5761 ggcatgtaga aggagaaatc gattatgaca agctcataaa acagtttggc accgaaaaaa
     5821 tcactggaga actgctaaaa aaaatcgaaa aagtcactgg agaaattcat tttatgctaa
     5881 gacggggcat ttttttctca cacagagatc taggcaggct tttagcagac tatgaaaaag
     5941 gcaaaaaatt cttcctatat acgggccgcg ggccttctgg acacactcac attgggcatc
     6001 ttgtaccttg ggtttttgca aagtggcttc aggaaaaatt tgacactacc atgtactttc
     6061 agctcaccga cgatgaaaag ttctatgcaa agcaagatct caccatggag cagacaaaaa
     6121 agtttgccta tgaaaacgcc ctggacttta ttgctctcgg atttaagcca gacaagacaa
     6181 agataatcat tgattcgcaa aacatcaaaa cgctttatcc ccttgcagtc caagttgcaa
     6241 aaaaaatcaa tttttcaaac acaaaagcag tctttggttt tacaaacgaa acaaatgttg
     6301 gcatgatttt ttacacgtct ctccagtccg ctccgtgctt tattgaagac aggcctgttc
     6361 tgatcccact tggtgtggac caagacccgc actttagaat cacgcgcgat gttgccccgc
     6421 gaatcggaag gccaaagccc gccctgattc acaacatcat gattccatca cttacaggac
     6481 ctagagggaa aatgtctgcc tccgaagaaa gtggaaccat ctacactacc gactcgccag
     6541 aaatgataaa aaagaaaatt aacaagtacg ccttttctgg cggccagcca acagtggaag
     6601 aacacagaaa gcttggagga aatccagaca tcgatgtgtc gtaccaatac ctccgaattt
     6661 tctttgagcc agacgacaaa aagctcaagc agattcatga cgactacaag tccggcaagc
     6721 tgctcacagg cgagctaaaa gcaattctga ttgaaaaggt cacagaattt ttaaaatcac
     6781 accagcaaaa acgcgaaaaa gcaaaaggac aacttgaaaa atttttgttt gaagactaga
     6841 tgaaaataaa aattgcctgc caagacaaac acgaagcctc caagcttgca agcctagttt
     6901 tcataaagga tggaaaagag acgttcatct catcaatttt gaacatatat gacaacgagg
     6961 tagtaattgc gctaaaggac aaatccgccc attcaattgt tctaaaggac gactaccaag
     7021 tagagttgtt tgcagacttt atccaatctg tccttgatgg gatcagtcag ataattgacg
     7081 caaaggcaga aaacgacacc gtggaaataa caaagggtta gactagagat ttatccatct
     7141 catcagatag tgtttcttga acctttagaa agtaaagcct agtagagttt ggcattttta
     7201 tcgagttgtt gtctactgcc atcataaagt aacgcactgc cctttttgag atgtccaaat
     7261 tgaacttcat cggtagaatt ggatcctgct ttaccttgat tttgtccgca agccatggcg
     7321 gggccatttc ttttgcctct gcaattattg tagagtacca aaaggagagg ttttctggat
     7381 gaaggccttc ttggagacca gttacgtttt ttgtaaattt tttcatcata ctattgatga
     7441 tgctcaacga ttcggattga gaatttttgt tttatctgaa taaatcaaac ataggcaatc
     7501 agtttgtcaa ttatagcaag tttccttgcg catcgatttc agaatttcgt atcctagtag
     7561 tagaaatcct ttgtccgtcc tttgctagtg ccattggaac gacaaccgtt tcaactgggc
     7621 cgaggttttt ttcctccctt agcttgttta gggttttgcc cttgtgtgca gtctcctcgc
     7681 ttactattag tgcctgaacg tttccctcaa ttgccgcagg cccaaagtcg ttgtcaagct
     7741 tgctgataag atattccttg ttttgaaaat tcttctcgat gaattgtttt agtgtatcaa
     7801 gcctttgcaa atatggatga ttcaggtttt tgcccttttt tttggcaagc tcatcacttg
     7861 taaggccgat tatcaccttt tttgatgcag agaatgccgc ttcaagcaac gcgatgtggc
     7921 ctttgtgaat gatatcaaat gtacctccca ttgccactac atcaaatcga gtcatgagat
     7981 gagttagtgt tcgtgtggat aaatctatga tgttacaaga acaattgaga ttgtcttgct
     8041 tggtgctgca agtgcgacgc cgttaggatc ccacacatat gcttcgataa agtatcctcc
     8101 ctcacgttct ggagtccagt tgacagaggc agtctggggt acggcggagc taaactcacc
     8161 ttctgcaatt cctaaaaact ccactggtgc cgtttcacca aattgtttta cttgtgcata
     8221 atacacatac ggctgctttg atcctgacag ggcaaagtac tggattgaaa gatcacttgc
     8281 tatgtcaact ggttcgccga ctttgagctg cgagacgcgt tttccggata aatccatcac
     8341 acccacatcg ttaattgaaa tcagtcttgt tagtgtatca agtgaaatgt cggtaacatc
     8401 ggtaatcggc gattggtagt tatcagactc tgctaaaact ttgaatgttg ttgcacgata
     8461 atccatcagt gcatcaaact cgatgtttac gctttgtcct tgcgttattt catcaattgt
     8521 cagtgtctga attccaacta ttcttggggg tttgaatgcg tcaaacgcga ttagatagac
     8581 gttagtcttt gtgctttcct gcgcaccatt gtttttgatt tccgctgaaa gcttaatcgt
     8641 gttgccgatg gctagtgtgt caggcgttat ttccagcatt tgttgttttt gcgtagcagg
     8701 gttgaaccca agtatgttaa ttgtcacttt tgagatttct ggatttgcag tctttgattt
     8761 tatcataaag ggggacttgc cgcgagcagg tatgacatca agcaaggtac ttccagttac
     8821 cgtttctaga ggagcttctc ctgagccaga cgtttttccg ctgtaaaatc cgacccagac
     8881 tttgatgttt gttacaggaa aggtcgttgt gttttctact tcgccaatca ctattgtata
     8941 tccctgatcg tctttgtagc taaacggggt agtgttagtt agttttacca ccagtgtaga
     9001 tggcgtatcc gttacctgtc caaaaccctg agatacaggc aacaacagaa atgcaaaaat
     9061 ggatatgacg agtatctttt tcatttgatt ctagttatca tatgggcatt aatcacataa
     9121 gaaacattat tctaaacaat gtggactaga agcttttaca agataggaat aaagaataaa
     9181 agagaagaaa cgagtgatta tcgtcttttg cttctcatac cagttatgac aaaccagtaa
     9241 actaatcctg gcgccaaacc gacgataagt ggtatccata cagcgattga ttctgctgca
     9301 aatgcttgag ccataccgag gttcctttca aaatgtcata tttaagtcca tcctttgccg
     9361 tttggaatcg cagaagatcg gaggttgtaa gtggactgaa ggggatttga acccctgacc
     9421 tcccgcgtgc aaggcgggca ttctaccgct gaactatcag ccctcaaaaa gagattttgg
     9481 tgtgtggatt ttaattgttg tctttgattt gggcaagaat ttttatttgc aaaaattaga
     9541 gcgcaacaca tggtcgtaat ggaatctcaa gtccagctaa aaaaaggaga tacagctcca
     9601 gacttcgagt tgcttggagt cgatgacaaa aaacactcac ttgcaagcta cggcaattat
     9661 gatgcagtgc tggtaatttt catttgcaat cactgcccct atgtcaaagc caaagttgac
     9721 gcaataaacg aggtcgcaac caaattcaag gacaaagtag cagtaattgg aataaacagc
     9781 aacgactcag tagcatatcc agatgatagc tttgagaaca tgaaaaagtt tgcagctgaa
     9841 aagaaaatcg ccttttcgta tcttgtagac gatgcacaac aggtagcaaa gcgatacggc
     9901 gcagtctgca cacctgaccc gtttttattt gacaagcaaa gaaagctcgc atttcatgga
     9961 agaattgaca acgctatgaa gccagaggat gtggcaacgg aaaaaacaat gattgcaaac
    10021 atagagagat tgttatcagg ccaaccaatt ccaaaagatt ttgatccgtc gattggttgc
    10081 tcaataaagt ggaagagttg agccacacat ttaaaatacc gatctcttgg aaattttttg
    10141 agggccggta gctcagcttg gctggagcgt tcgactgata aaacaacctt tcagacatcg
    10201 aaaggtcgag agttcgaatc tctctcggcc caccattttt tgtgccatac aaaattacgg
    10261 tatgagtctt ggcacgacca tgtagatgag taaaatgatt tcaagtcttc caaatatcat
    10321 cagaaacgaa aacagtgtaa gtgtagttgg gtctagatct aagccgattg taccagcacc
    10381 aagtccagtt gtagtcagag cggagaccgc atcaaagtac gaatcttcaa agtcataacc
    10441 taaggactcc atgtggactg cgacaaaata tggaattaat ggaaacaaaa caacgaggat
    10501 tgatgctgta atgatatggt ttttgtctga atgagatagc ttttttctca aaataagatt
    10561 tttgacgttt acaagtttaa tcaatctaaa taccttcatt cctccagccg ttgaaaatcc
    10621 gcatccacca attatcatga taatgataaa aacaattcct ggaattggac tgagatcctg
    10681 caagttgatc gtttggaatc cagtgttagt gctagctgaa atggtattga atacggcatc
    10741 tattggattc acatccatag aaa
//
LOCUS       DASWKH010000103         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1765845, whole genome shotgun sequence.
ACCESSION   DASWKH010000103 DASWKH010000000
VERSION     DASWKH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765845"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VGA92_07875"
     CDS             <1..404
                     /locus_tag="VGA92_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX9846360.1"
                     /translation="KVDVVILAAAASDYTVLNPSSSKIKSSKKSLQIKLVQAPKIIDH
                     IKKWQKNVFLVGFKAETNISKKKLASLARKKLAEAKADLIVANDIGTKYQKNPDLNEI
                     VLVNSKKAVFLSRRRKEKLAKLIRKELEKVI"
     gene            complement(469..966)
                     /locus_tag="VGA92_07880"
     CDS             complement(469..966)
                     /locus_tag="VGA92_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846361.1"
                     /translation="MKQKVKQLEAEIVQLKSQPEEPTSPADCGPNQIFQGGQCLDIEK
                     EEPEDCAPNQVYQQGKCVDIEEEGSKAFTIKTDQPSYDDGKIIRITGNVGSINESYPN
                     TSITIIITGPTGNIVAIMQVIPDTTGNFSHQVIAGESMTDAGYYTVTAQYGTQKATTT
                     FSFTA"
     gene            complement(1403..2482)
                     /locus_tag="VGA92_07885"
     CDS             complement(1403..2482)
                     /locus_tag="VGA92_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9846362.1"
                     /translation="MILVTSLAPTISAQEQTVAIPDWIRTTALWWSDGEIDDSNFLSG
                     IQYLIDKKIMKIPATTKTIKPSLPFVPNWIKDTAGWWATGKVGDIDFVNGMSWLIENG
                     MIHVGQKAFCTGDKLCLVGEVKTIIDGDTIYLEKHKIRLSLTNTPEREEHGYFEATEF
                     TRKLCPVGSTILVDQDDLQPVDQYGRVLGKVFCGDKLLNEELLINNHGWILDQYCKTS
                     EFSSEPWAKKYGCLSKNIPLPIEPKQTSNSFDVEVTGEKQVRRGTTQTLEVLVSRNNI
                     PVEDAWVVIRIEDYGEDIIREFDGYTNKNGYFEFSWEIPKSFDDIETLLAFVDVTDDI
                     SAKTELFKFQVYCLPGEAGCKVEGN"
BASE COUNT          813 a          538 c          453 g          874 t
ORIGIN      
        1 aaaaagttga tgttgtgatt ttggcagctg cggcatcaga ctatacagtt ttgaatccaa
       61 gtagctctaa gataaaatcc tcaaagaaaa gcttgcaaat caagctagtc caagctccaa
      121 aaattataga ccatatcaaa aaatggcaaa aaaatgtttt tttagtcgga ttcaaggcag
      181 agacgaatat ttcaaagaaa aagcttgcca gtctagctag aaaaaagctt gccgaagcta
      241 aagctgattt gattgttgca aacgatattg gaacaaagta tcagaaaaat ccagacctta
      301 atgaaattgt tttagtaaat tcaaaaaaag ctgttttttt gagtcgtaga agaaaggaaa
      361 agcttgccaa attaattaga aaagagcttg aaaaagtaat ttagcttcat tttagacaat
      421 attttgtact tgttacaata attggaggga tgctttacgt tcttattatt atgctgtaaa
      481 tgaaaatgtc gttgtagctt tttgtgttcc atattgagct gttactgtat aataaccagc
      541 gtctgtcata ctttcaccag ctataacttg atgagaaaag ttacccgtag tatctggtat
      601 tacttgcata attgcaacaa tattgccggt tggaccagta attataatcg taatggatgt
      661 gttaggatac gattcattaa tgcttccaac atttccagtg atcctaatta tctttccatc
      721 atcatatgat ggttgatcag ttttaattgt aaaagctttt gacccctctt cctcaatatc
      781 aacacattta ccttgttggt aaacttgatt tggagcacaa tcttcaggtt cctctttctc
      841 aatatcaaga cattgacctc cttggaagat ttgatttggt ccacaatctg caggtgatgt
      901 tggctcttct ggttgacttt taagctgaac tatttcagcc tcgagttgtt tcactttttg
      961 tttcaatgat tctatttgtg gaactttgat aatgtcttga tcaataagaa atgataagcc
     1021 ttcaccaaac tcatcgtctg aaattttatc ttcagcccaa aatccagcta cacccttaac
     1081 ccatgccgga attgagtatt tttcctaggc actaacagta gaagcaattg ttattgaaat
     1141 aatcaaggat gctacaatta ttggaatcca aagctttgcc caattaccca atgttaaatt
     1201 atgttcatac ttttagataa atcagatctt gatttttcgt ataatcacag caagattatc
     1261 cagaacgtaa atgaaaatta acgcaacaat cacagtcatt cataatacat tcttggttaa
     1321 ttgcatgtcc gcaaattcca catgcgcagt gtattttcat gatactatga tgcagctttt
     1381 tcagatataa cggatctgtg ttttaattgc cttcaacttt acagccagcc tcgcctggca
     1441 ggcaatacac ctgaaacttg aatagctcgg ttttcgctga aatgtcatct gtcacatcaa
     1501 caaaggctag tagggtctct atgtcatcaa agctttttgg aatttcccaa gaaaactcaa
     1561 agtagccatt tttgttcgtg tatccatcaa attctcgtat gatgtcctcc ccataatcct
     1621 ctattctgat aacaacccaa gcatcctcaa cagggatgtt gtttctacta actagaacct
     1681 cgagggtttg ggtcgtgcca cgcctcacct gcttttctcc agttacctca acgtcaaaag
     1741 agttggatgt ttgttttggt tcaattggaa gcggtatatt ttttgaaaga catccgtatt
     1801 tttttgccca aggttctgat gaaaactcgc tggttttaca atattggtct aaaatccatc
     1861 cgtggttatt tatcaaaagt tcctcattta gcagcttgtc cccacaaaac actttgccca
     1921 aaactctgcc atactggtct actggttgca ggtcgtcctg atcaactagt atggtagagc
     1981 caacaggaca aagttttctt gtaaattctg tggcttcaaa atatccatgc tcttctcttt
     2041 ctggcgtgtt tgtcagagag agacgaatct tgtgtttttc aagatatatt gtatcgccat
     2101 caatgattgt ctttacttct ccaaccaaac aaagcttatc tccagtgcaa aaggcctttt
     2161 ggccaacatg aatcatccca ttttcaatta gccagctcat cccatttaca aagtcaatat
     2221 cgcctacttt accagttgcc caccacccag cagtgtcctt aatccagttt ggaacaaacg
     2281 gcaaagatgg ttttatggtc tttgttgtgg ctggaatctt cataattttt ttatcaatga
     2341 ggtattggat tccagaaaga aagttagaat cgtcaatttc accatctgac caccacaacg
     2401 ctgtggtacg aatccaatca ggaattgcaa cagtttgttc ttgcgcagat atagttggag
     2461 ctagtgaagt tacaagaatc aagctaagaa aaattgcaat tgttttcagt tcaaaataat
     2521 gataatttct catccataat caatcttttt tatccgaacc tgactcccaa acgattccag
     2581 ataatgtccc aaaaaattct ttatctgctc gccaattcga aacccaagcg caccatccat
     2641 tatgccagat ttttctatta cagaaaacat ttcggaac
//
LOCUS       DASWKH010000104         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3236116, whole genome shotgun sequence.
ACCESSION   DASWKH010000104 DASWKH010000000
VERSION     DASWKH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236116"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..507)
                     /locus_tag="VGA92_07890"
     CDS             complement(55..507)
                     /locus_tag="VGA92_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX9846363.1"
                     /translation="MLSHQDASWLKLWQQNSPEIRKRAIQWRKDNAFTRIVRPNRIQK
                     ARRLGYKAKQGMVVIRTRVGTGGMRRQRPVAGRRPKHLGVTRIKADVNMQQVAEKRTQ
                     QRYKNLKLLGSYFLYKDGFHYWFEVIMADKAHPRISKDKELRKRLIPA"
     gene            746..1429
                     /locus_tag="VGA92_07895"
     CDS             746..1429
                     /locus_tag="VGA92_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846364.1"
                     /translation="MVEKYLHRKDDEDEDEVSHIAELGLKLDDSTGSIDPMEVKARTI
                     TFGKEVSNATKDAVIKEKSEKFKDQMSNTNQYLKFINAHLAEKKSKIDKLKESERRFK
                     EEIESLQNDQVKPRAELDKVNYKYITENDTKSLLSHLETERHTLQEKLSHQEQQIKNT
                     AQQIQQKDEHILQIKNELDSLVKKNSQESQDPIAVIKEELAKLGIKDDSAKILDAVNS
                     LSSLINSKK"
     gene            1486..2085
                     /locus_tag="VGA92_07900"
     CDS             1486..2085
                     /locus_tag="VGA92_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846365.1"
                     /translation="MKLLAFVFLVGVLFGASAFAVEPIQISTSQQTYHYGDYLSVTIQ
                     VSEVTGSDGVMYIIDSDGVKSSSIPLKIRDNITTIVAQSPFDPLLFKEGKYQIQLDYD
                     GTKLFADFELVDAGNTMMPYGSNVVVPQWVVGTISDYNFLKFLADKNVISLSPDYALK
                     ESVKIPTWYKTNAAWWSEKKISDVEFVNGLQYLLSKKII"
BASE COUNT          690 a          457 c          429 g          621 t
ORIGIN      
        1 tgggcaaaaa caggcatcat gattaacgga attagcaaaa gtagggtttt caacttatgc
       61 cgggattaat cgctttctta gctctttgtc ctttgatatt cttggatgag ccttatctgc
      121 cataatcact tcaaaccagt aatgaaatcc gtccttgtac aaaaagtacg agccaagcaa
      181 tttaagattc ttgtatcttt gctgggttct tttttctgca acctgctgca tgttaacatc
      241 tgctttgatt cttgttacac caaggtgttt tggtcgtctg ccagcaacag gcctttgtcg
      301 tctcatgccg cccgttccga ctcgcgtgcg aatgacaacc attccctgct ttgccttata
      361 tccgagtctt cgtgcctttt gaattctgtt tggcctcact attcttgtaa aggcattgtc
      421 ctttctccac tggatagctc tttttcttat ttcaggcgaa ttttgctgcc ataatttgag
      481 ccatgatgca tcttggtgac taagcattat tcggcactat tgtaaacccc ttaataatct
      541 ctacagaagc cggcgccatt ctagatttgg gtttgattaa aaatgtctgc cagagtcaga
      601 attctcctag tttttttcac tggcaggaat ttcggttctg aaatccagcg agattagaaa
      661 ctatggcgtc ataaagatat ccaaaggttg tgtgtttttt ttaaacgaca gccaaatcat
      721 atcggtgatt ttgtaaccat acataatggt tgaaaaatac ctgcacagaa aggacgatga
      781 ggacgaagac gaagtatccc acatcgcaga gcttggccta aaacttgatg attcaacagg
      841 ctctattgat cctatggaag tgaaggcaag aaccatcact tttggaaaag aggtctctaa
      901 tgccacaaag gacgcagtaa tcaaggaaaa atccgaaaaa ttcaaggacc agatgagtaa
      961 cacaaaccag tatctgaaat tcatcaatgc acaccttgca gaaaaaaaat ccaagataga
     1021 taagctaaaa gaatccgaaa gacgattcaa agaagagatc gaatcactac aaaatgacca
     1081 ggtaaaacca cgggctgagc ttgacaaagt caactacaag tacatcacag aaaacgacac
     1141 aaagtcgctg ctttcccacc tagagacaga gcgccacaca ctacaggaaa agctttcgca
     1201 ccaagaacaa caaatcaaaa acactgcgca gcaaatacag caaaaagatg agcacatact
     1261 ccagataaaa aacgagcttg acagcctagt taagaagaac tcacaggaaa gtcaagaccc
     1321 aatcgcagtc ataaaagaag agcttgcaaa acttggcata aaagacgatt ctgcaaaaat
     1381 acttgatgca gtaaactctc tttcaagttt gataaattcc aaaaaataat atcattgtat
     1441 gatttgttag gaacgttttt ttgtagcagt atttgttttt acaatgtgaa actgttagct
     1501 tttgtttttt tggttggagt tttgtttggc gccagtgcct ttgctgtaga gcccattcaa
     1561 atcagcacta gtcagcagac ataccactat ggcgactatc tatcagttac tatccaagtc
     1621 tcagaggtaa ctggtagtga tggagtcatg tacattattg actcggatgg agtaaagagc
     1681 agttccatac cactcaagat aagagacaat attaccacaa tcgttgcgca aagtccgttt
     1741 gatcctttgt tgttcaaaga agggaaatac caaatccagc tagactacga cggaacaaaa
     1801 ctgtttgcag attttgagct tgtagatgca ggaaacacca tgatgccata tgggagcaat
     1861 gtagttgttc cgcagtgggt agttggtaca atttctgact ataattttct caaatttctt
     1921 gcagacaaga acgtaatttc tctttctcct gactacgccc taaaagaaag cgtcaagatt
     1981 cccacatggt acaagactaa tgctgcctgg tggtctgaga agaaaatctc cgatgtagag
     2041 tttgtaaatg gccttcagta tcttttgagt aagaaaatta tctagtgcta gttatttttt
     2101 ctgctggctc ttccgattgc ccacaggatt atcattatga taaacgctat gacaaacgct
     2161 gcgactagac catatatcat ctgaaatgca ccactac
//
LOCUS       DASWKH010000105         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3575357, whole genome shotgun sequence.
ACCESSION   DASWKH010000105 DASWKH010000000
VERSION     DASWKH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575357"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..190
                     /locus_tag="VGA92_07905"
     CDS             26..190
                     /locus_tag="VGA92_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846366.1"
                     /translation="MRDKMEEEILKLKGKAAEAFLEYDSRNLTAKEQRRIKKARKYYL
                     SYATNLNGFL"
     gene            243..689
                     /locus_tag="VGA92_07910"
     CDS             243..689
                     /locus_tag="VGA92_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9846367.1"
                     /translation="MEDVAIRSASKKDIPIILELLYELGRPKPKKDSQLDAFEKLITK
                     YLSDSDKEILVAQNQGGVVGMVSIMYLHRLNQEFFEMYIPELIVSKDHQNKGIGKKLI
                     EACIKLANDRKCHRIRLESGNQRKESHIFYKKLGFDQSALSFTKNL"
     gene            762..1376
                     /locus_tag="VGA92_07915"
     CDS             762..1376
                     /locus_tag="VGA92_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846368.1"
                     /translation="MKYFMILLTVILAFFVSVQAFASHDPNQPQLHSIIIPPDYPPLK
                     QFKAGLSAEKIICRQGLELVTKYDGSPACVKPETKVKLIERGWTKSTEHVDDVGEFWG
                     SIVGVVMLGPTCPVVENPLDPECADKPYETTLVVTTADQSRVIKEFSSDANGKFSVEV
                     SPGEYAIRSAAAANVLPYCSSNDTIKLSANDYTEITVHCDTGIR"
     gene            complement(1430..1711)
                     /locus_tag="VGA92_07920"
     CDS             complement(1430..1711)
                     /locus_tag="VGA92_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846369.1"
                     /translation="MVTEAQIRASILFKLHKRGNWGASHTAFDNIKKGFNERDFGKHG
                     TKIIDKIAKELVREGLIITKPTHYGRQVSLNPRQSDRIVTIMKAYFPDV"
     gene            1772..2119
                     /locus_tag="VGA92_07925"
     CDS             1772..2119
                     /locus_tag="VGA92_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846370.1"
                     /translation="MLYKRNETLLLKYLGPSPVLRIIDFFLDNPLFDYSKNDIIKNLE
                     MGRVTFFKYWAELEKSGAVKVTRRVGRATMYKLDRENEVVKHLVKLDMALAKRTMEKA
                     SEQYKKPITVKSR"
     gene            2213..2833
                     /locus_tag="VGA92_07930"
     CDS             2213..2833
                     /locus_tag="VGA92_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9846371.1"
                     /translation="MSEQDSANKSYLSRRDFLKLLAAGGVAIAFTPFVPWGSYGPNPQ
                     LDNLEKTKVILPGGTHANIKTFPVNYAEVVTYPNTGDSVLDEESFRKWQLIRLPAELG
                     GNANDVSAFRLYSMVCLHLWCLWSYFPTTDPTKPLNKGACPCHASTYDPLTGMATDGP
                     AAKQSPPSNILPRLDLEADANGFLWILPPKWGVNANGVIGYGRYAK"
     gene            2955..>3288
                     /locus_tag="VGA92_07935"
     CDS             2955..>3288
                     /locus_tag="VGA92_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846372.1"
                     /translation="MSQGLIISSVNRRIVFAGIFLASLSGLVLEIAITRIFSAAIWYH
                     FAFVAVAVALVGLGSSGLLVHYRLKKIKENWAGDLTVLACLGITSVIPITLFVMHSLA
                     SQLIYLPVF"
BASE COUNT         1060 a          631 c          657 g          940 t
ORIGIN      
        1 ttaaagtctg gaaagggtct tttaattgcg agacaagatg gaagaagaaa ttctaaaact
       61 aaaaggaaaa gccgcagagg cctttctaga atatgactct agaaatctta ctgccaaaga
      121 acaaagaagg ataaaaaaag ctcgcaagta ttacctgtct tatgcaacaa atctcaacgg
      181 gttcctttga gtccgaaaaa attttaaaaa ctccttaaac cacaattcat tttatcataa
      241 caatggaaga tgtagcaatc agatctgcct caaaaaaaga catacccata attcttgagc
      301 tgctttatga acttggaagg cctaaaccaa aaaaagattc acagctagat gcttttgaga
      361 aattaatcac aaaataccta tctgattcag acaaggaaat tctagttgcg caaaatcaag
      421 gaggcgttgt agggatggtt agcataatgt atcttcatag gctaaatcaa gaattttttg
      481 aaatgtatat tccagaatta atagttagca aagatcatca aaacaaagga attggaaaga
      541 aactaatcga agcttgtatt aagcttgcaa atgacagaaa atgccataga ataaggcttg
      601 aatctggcaa tcagcgaaaa gagtctcata ttttttacaa aaagcttgga tttgaccagt
      661 cggctctgtc ttttacaaag aatctataat cttgttaatg attttacaat aacaaagctg
      721 agaaaaactt ttcaatgatt gtttttggag ctatctgaat catgaaatac tttatgattt
      781 tgttaactgt aatacttgct ttctttgtat ctgtacaagc atttgcgagt catgatccaa
      841 accaacctca acttcattcg attataatac ctccagatta cccaccacta aagcagttca
      901 aagcaggact ttcagctgaa aaaataattt gcaggcaagg actcgaactt gttacaaagt
      961 atgatggttc tccagcatgt gtaaaaccag agactaaagt caagctgatt gaaagaggtt
     1021 ggacaaagag caccgaacac gtagatgacg ttggtgaatt ctggggaagc attgtaggtg
     1081 tcgtgatgct aggccccaca tgccctgtgg tagaaaatcc tctggatcca gagtgtgccg
     1141 ataaaccgta cgaaacgacg cttgttgtga ccactgctga tcaatcacgt gttataaaag
     1201 agttcagctc cgatgcgaat ggaaaattca gtgttgaagt atctcctggt gaatatgcaa
     1261 tacgttctgc tgcagcggca aacgttctgc cttattgctc atcaaatgac acaataaagc
     1321 taagtgctaa tgactatacc gaaatcactg tacactgcga cactggaatt agataataaa
     1381 attcttcaaa ataaaatctg gtaggagttt tgcagccact tgaatatact taaacatcag
     1441 gaaaatacgc tttcatgatc gtaacaatcc tgtcgctttg ccgtgggttt agtgaaacct
     1501 gcctcccata gtgcgttggt tttgtaatta tcagcccttc acggacaagc tcttttgcaa
     1561 tcttgtctat gatttttgtg ccgtgctttc caaagtccct ttcgttgaat ccctttttta
     1621 tgttgtcaaa tgcggtatgg cttgctcccc agtttcccct cttgtggagc ttgaaaagaa
     1681 ttgatgctct tatctgagcc tctgtaacca tgagttcact tacagtactt tgagagtcta
     1741 ggattaaata ctttgattac aaactgattt tatgctatac aagcgaaacg aaacgctttt
     1801 gctaaaatac ttgggcccaa gccccgtcct gaggataatc gacttttttc ttgacaatcc
     1861 gctttttgac tattccaaaa acgacataat aaagaacctt gagatgggca gggtcacatt
     1921 cttcaagtac tgggcagagc ttgaaaagtc aggagcagta aaggtgacaa gaagagttgg
     1981 gcgagctaca atgtacaaac tggacagaga aaacgaggta gtaaagcatc ttgtcaagct
     2041 ggacatggca cttgcaaaac ggacaatgga aaaggcttca gagcaataca agaaaccaat
     2101 tacggtaaag tctagatgac tttgaacaaa taaaaaattt ggataggagt tttgtagtgg
     2161 gaaaataaat tcagtagtat gattaataga taagaaacac attttgtaaa acgtgtcaga
     2221 acaggattct gccaacaaga gctacctgtc acggcgtgat tttttaaaac tgcttgcagc
     2281 gggcggagta gcaatcgcat ttacgccgtt tgttccatgg ggaagctacg gcccaaatcc
     2341 tcagttagac aaccttgaga aaacaaaggt aatcttgcct ggaggtaccc atgcaaacat
     2401 caagacattt ccggtaaatt acgctgaagt tgtaacctat ccaaatacag gtgacagtgt
     2461 tttagatgaa gaatcattta gaaaatggca gcttatccgt ctgcctgctg agcttggtgg
     2521 aaatgcaaac gatgttagcg catttcgtct atacagcatg gtttgcctgc atctttggtg
     2581 tctgtggagc tactttccaa caacggatcc aacaaagcca ctaaacaaag gggcatgtcc
     2641 atgtcatgca agcacctacg atccacttac tggaatggca actgatggtc ctgctgcaaa
     2701 acaatctccg ccatcaaaca tacttccaag gcttgaccta gaggcagacg caaacggctt
     2761 tctctggatt ctgcccccaa aatggggtgt taatgcaaac ggcgtaattg gatatggccg
     2821 ctatgccaag taaaatttaa tctttagatt tttgctaaag tttaatcaac cttacttaaa
     2881 accagaaacg acgagttaat aaaaaattct tttgaaagaa taatattcaa ccataatgcg
     2941 agtcagtcag tttaatgtct caaggattaa taatatcgtc agttaatcgc aggattgttt
     3001 ttgctggaat atttctggcc tcattatctg gtcttgtttt ggaaattgcc ataacacgaa
     3061 ttttttcagc agctatttgg tatcactttg cctttgttgc agttgctgta gcgctagtag
     3121 gacttggttc atcgggatta cttgtccact atagattgaa aaaaatcaaa gaaaattggg
     3181 ctggcgatct cacagttcta gcatgtcttg gcataacatc tgttattcca ataacccttt
     3241 ttgtaatgca ttcgctagca tctcaattaa tttatctgcc ggtattca
//
LOCUS       DASWKH010000106        35540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3239682, whole genome shotgun sequence.
ACCESSION   DASWKH010000106 DASWKH010000000
VERSION     DASWKH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 35540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35540
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3239682"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1011
                     /locus_tag="VGA92_07940"
     CDS             274..1011
                     /locus_tag="VGA92_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9846373.1"
                     /translation="MVRLGILQTRSYPTNQKGIDEVSKMLIGAAKSETDIICLPEQWQ
                     KENKFVDFDVEFAQLKKIAREYAMTIIPGAFYQKKKKGFVISAPVIDSSGDIIGTQEK
                     IHPFDYEKSLIRAGTKARVFKTACKFGIIICYDMVFSDVAESLVKKGAQVLFSPSRIV
                     KRGIIPWHMYVQVRALENRIPILATNIQNNRFGGNGIIVDLTEDDNVMIPKITTLHGQ
                     TTKSSSFDLSKYEKSRKHRYLDHRRFA"
     gene            1053..1994
                     /locus_tag="VGA92_07945"
     CDS             1053..1994
                     /locus_tag="VGA92_07945"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEX9846374.1"
                     /translation="MIEPGKPVQDINITNQTNIEEIFAMMSKSGGFESRNLADGLEIL
                     SSMITDKNCLRFLSFVAAITSTGTRGIIKDMIQKKWFDVVITTCGALDHDIARHFSNY
                     REGSFTMDDSQLADQNIHRLGNVLVPMESYGPLIEEKVQAILEEAYSQGKKEMSTADI
                     THEIGKRLGEDSFLYWAYKNNIPVIVPGIMDGAVGSQVWMFSQKHRDFKLNMTADADI
                     ISGLVFKAQKSGALMLGGGISKHHTLWWNQYRDGLDYAVYITTAQEFDGSLSGALVRE
                     AISWGKVTQGAKQTTIHAEITTTLPFLYRALLSKIKQ"
     gene            2043..2759
                     /locus_tag="VGA92_07950"
     CDS             2043..2759
                     /locus_tag="VGA92_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9846375.1"
                     /translation="MLPRLWVKELKQIDIEGGFLIDGFPSIGFTSAIASESLMHTSQY
                     DLVGVIDSIDFPTVTILKDAIPNYATRIYVNSDLKVSIFTSYLTINEPYHRAIAKMML
                     SWAKKHKCSLVVSSSPMKFPIDRPDEIIAAGSTDSARETLKKAGMTLLQSGTIPGIPG
                     ALLNDGMLNGQDVIVVLVNVDEMGPDFKSSAHLCMAMSKLLPGVSCDLTMIQKQAEIA
                     EKQIKETEKETRTLKESMYR"
     gene            2775..3392
                     /locus_tag="VGA92_07955"
     CDS             2775..3392
                     /locus_tag="VGA92_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX9846376.1"
                     /translation="MQEFFGFFAAVVVISASGVMAPGPLFAATIASGIKEGKLAGLKV
                     AVGHTVVEFLLVILIGIGVLSLEVLPEFRVVIGVLGALSIFAFASLQLHSTLKKQGHV
                     QKESKYSSFLTGILLTGLNPFFLIWWFTIGFKLIADAFSLWSFLGILIMFGLHIWMDY
                     VWLFFVSALSSKGTKFLTNKGYKFCMIAINVVLIYFGISFITNLS"
     gene            complement(3389..3622)
                     /locus_tag="VGA92_07960"
     CDS             complement(3389..3622)
                     /locus_tag="VGA92_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846377.1"
                     /translation="MQRHNYYKVSVVCGIVIISTLLFFQNFHSCSAKQFSIIDEIIEY
                     EKTMDPNICISLAEKIGDLNNECKSEFETPDCG"
     gene            complement(3609..4076)
                     /locus_tag="VGA92_07965"
     CDS             complement(3609..4076)
                     /locus_tag="VGA92_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9846378.1"
                     /translation="MSEQDKPTDAEEIFAESQKIKEPKDEQTETTTLVQETENTPAPQ
                     ETENTPAPQETENVLDEDSAKKTRDVIFVGIKPIMTYVTATLTQLSNQQVVTIKARGQ
                     RITQAVDVSQMIVKRMNTVGYIIKDIRIASDSLLSQDGKTRNVSTIEIDIAKA"
     gene            complement(4118..4366)
                     /locus_tag="VGA92_07970"
     CDS             complement(4118..4366)
                     /locus_tag="VGA92_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846379.1"
                     /translation="MSYPITSDEGGIKIIPELMKQEKMYYCLHNGKVLLVFKDSQEFL
                     NCYEIEEKEIVENITTCKSPDEIEKAIENYLAKQNLKH"
     gene            4444..5538
                     /gene="dinB"
                     /locus_tag="VGA92_07975"
     CDS             4444..5538
                     /gene="dinB"
                     /locus_tag="VGA92_07975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9846380.1"
                     /translation="MSERIVFHIDFDYFYAQCEEIRDQTLKTKPVCICIFSDRGGDSG
                     AIATANYIARKYGVKSGMPIRFAKSRLQQIPDAVFLGADFPFYSDISEKAMEVIKSYA
                     DVFEYVGKDEAYLDVTRKSEKSFQIATHLAQQLKNQIRNEIKMTCSVGISANKLVSKI
                     ASSFRKPDGLTVVEPHKTESFLNPLKISDIPGVGKITEEKFTEMNLKTIEDLKNVDVF
                     TLNKMFGRKISSYLYNAARGIDEEPVAERAPSIQYSRIVTLKEDSKDFEFLAKSLDEI
                     CDDLHHTILKHKKMFKSVGIQFVQSDLSNKTKSKMLKNPTFSIEELKKTALLLLEEAL
                     VDQTMNVRRLGAKVSELSDFEGQSSITNFF"
     gene            complement(5540..6457)
                     /gene="artG"
                     /locus_tag="VGA92_07980"
     CDS             complement(5540..6457)
                     /gene="artG"
                     /locus_tag="VGA92_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumarchaeosortase"
                     /protein_id="HEX9846381.1"
                     /translation="MQNKTLMLAILVIVSPVLFALAAYPDSFSLSWNQGRGGFIFAMA
                     FVVAELIGIKILISKKRLIAIIPLAAAVIVYLVSLEYGLRDYIINGAEQYNVNLVYSW
                     TWMWDFVVMGAFVMAAICILFGRRWIRIAPAGPIFLCGSAIILSLDAFFPYDTLGPLQ
                     YIVPHLVKANVSIINALDLGTAIARDNLMFLRGEHGSMALQVFWPSAGVHSVIIYSLV
                     MMAFLFKMNIPPKRKAVYFVIGILGTIGVNMIRIFSLSIFVLKVSANPTDFEEFHGIA
                     GEIMFLPWLFAYLFLVMAIETKRLKKQSA"
     gene            complement(6498..7043)
                     /locus_tag="VGA92_07985"
     CDS             complement(6498..7043)
                     /locus_tag="VGA92_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846382.1"
                     /translation="MVDFNYLDELARQIKTNRNYLEQIEEELKVVNMKLHELPLKKPT
                     ESTFAKMIGVEYDDQLDTLQKSKANLEAKRDELSNAIKNNTNKFITEMTSPDLVVPID
                     PKPTFKDGRIQFQYRNATKFHNLFEFLSELLGLSAPLVVKDVMLSSSEIIVKVSNEYE
                     AKQKFISTMSEIQKTLTIKKK"
     gene            complement(7052..8446)
                     /locus_tag="VGA92_07990"
     CDS             complement(7052..8446)
                     /locus_tag="VGA92_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9846383.1"
                     /translation="MAATTTAPLPPAPVLMTCFGHCGIGLGAESYRRLLLDVGADPLK
                     PVLKDSKEESRILKRYGSTILRFPGAYTGGETPLVPRALLVDLDPRAANLILQSYPDL
                     FALRDKHVIHGAGGAARNWAEGRSRFKNEITQKWDFTKQLAALSPEPVRGYTVPFAMG
                     GGTGSSFACSFLEFIRSNTKEHATIATLGLLPEFGWDPVILEAAAINIVMNLEYQIKY
                     SDCSILFSNKRLRELGHKYEKRVQKIPSIIDDLPKEHEVGWKDYKGMNLIAANAISMF
                     IASFARETEWDMSNYRTWLATKRPKMAIPWVIPVLPEDDQWGLNQITGGKNNTMDAII
                     ENLNKKQDALLFDIDENDVRNYGGPRDSCCALVKVKGDFDIKEREILKRTIKERFNIE
                     DSRMIFVKIPIMDREQANITIFVNTKAIGPKILEIAREAEESWDAYKEEYGKWGLTTE
                     DFKQSLMDVVHQFS"
     gene            complement(8637..10622)
                     /locus_tag="VGA92_07995"
     CDS             complement(8637..10622)
                     /locus_tag="VGA92_07995"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HEX9846384.1"
                     /translation="MTEADDAFALPRTLVLNDAMTCQTFLNGFWDAATLTCTNSNIGF
                     FISTGDTLDIPAGVTLLNPTGRTFGTSVGGIINNFGTINNFGTIQNLGGINNHCGGVV
                     NNSGTLSGKAIVIIPCPDLDLDGFTSDIDCNDNNVTVYPGAPELDDNLDNDCDGIIDE
                     GFAPPGSYYDEIDNTIKPCLPSTYQPDPNQTSCILADSGHFVSESGATAQLECLAGSY
                     QDQAGQTSCILAPAGSYAPGPAATESLLCSAGTFSLDVGSAQCDDAPPGSFVADTGAT
                     AAELCPIGTFQPSSGQSSCIPSDIGHFVAVTGATSQTECPAGSFQSMTGQSSCEPAPA
                     GSYAPGPAATESLLCPAGTFTSVEGSASCDLAPPGTFVSILGATQAELCEPGTYQPDS
                     GQSSCLLADPGFFVDTFGAASQQQCPDGTTSEAGATECIPIPPIGDPFCDGMTIDQLI
                     AIGTYNVIDNRDGHLGKNIKGTKGNDLILTSDAGNKVSAKDGNDCIIGGTGKDNLFGG
                     KGDDKIFGMGGNDKIHGQQGDDVLSGDDGNDVIWGGQGNDFIDAGDGNDRVHGNQGND
                     TIFGGEGNDWLGAGIGNDMVFGGPGNDKIFGRPGNDTLNGDEGNDRIHGGQGNDTLNG
                     GSDTDRCHGGQGNNVFDSCESKKPMPDEEDWSEESDE"
     gene            complement(10801..11961)
                     /locus_tag="VGA92_08000"
     CDS             complement(10801..11961)
                     /locus_tag="VGA92_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846385.1"
                     /translation="MDPTARDIRRSIEENWKAYLSKYGNLFSSDSISGSSPPSVFVGS
                     FGYPKVGVGPMVPPIHGDTSLLDLPERWLGKSLEEIVNFRLNLVRGIQKVSVLEPKGQ
                     LIENLQELAMASNTPDSDIVFQKNTMPITTIDGESAPFGPIGEIKTAKFSGMSSNKVI
                     EQSYYDKDLKAEDAVLQLYNSGIEISKIQKCFSIGMFGKKRKLVPTRWSITATDNIIS
                     KNLVAEILDYSQIDSYLVFTHEHLGNLFSILLFPHHWIFEMEEAWYTESGQIGFGVDA
                     EDANGIDHYPSIAGAYFAAKLGIAEYLKKKKRQAGVLVLREIRPEYAVPVGVWQVREA
                     IRAAMSKPPTLVDTLELGLNLACKNMSISKNEWLSKGSTLKMLKQKSITDYF"
     gene            12054..12974
                     /locus_tag="VGA92_08005"
     CDS             12054..12974
                     /locus_tag="VGA92_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX9846386.1"
                     /translation="MVAKRIVSFLPSATELLYELGAGDHLVGVTHECLYPEQAKTKPR
                     VINSVFDPEKMTSRQIDQKISALTREGKNIFVVQEENLKKANPDLIIAQGTCAVCSAY
                     TNEVNKALAILEKKPDVEVLDPHTIEDILSSVMIVAKKIGKESEGQKLVESLKNRIDF
                     VKNKNFKIRSKILCIEWVEPFFTSGHWIPQMVEIAGGANLISTVGQHSQRMTVDEVEK
                     ADPDIIVMMPCGFDTKRTATECAAVLQNDQRWQGLRAVKDNKVFAVDANSYFSKPSIR
                     TITGIEILAKIIHPEECSALSVPDGAFRQI"
     gene            complement(13355..13825)
                     /locus_tag="VGA92_08010"
     CDS             complement(13355..13825)
                     /locus_tag="VGA92_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846387.1"
                     /translation="MNATKLVFSNYGYIGLAVAIFMVMFFLLSTLSEYVFFEPYFVFY
                     VPDDRVLGFALILAVSVLSAAVIPMNVYRVKILQRVSKASGGFLGSLIGASAGACSCG
                     PVGFAIISTFGTAGGIATAFLASYEIPLRIISTGILGYVYYTTAKSLSVECKAR"
     gene            14044..14334
                     /locus_tag="VGA92_08015"
     CDS             14044..14334
                     /locus_tag="VGA92_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846388.1"
                     /translation="MGLVFDKFITALFVIILGSAWFLTRRYESSRLYKIVAYDVFGSQ
                     VRLDGIRTTFNTHSVAISFAKHYREIFPHYDFALKSEMPNIKRGFLPKKTQI"
     gene            complement(14325..14687)
                     /locus_tag="VGA92_08020"
     CDS             complement(14325..14687)
                     /locus_tag="VGA92_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2-type zinc finger protein"
                     /protein_id="HEX9846389.1"
                     /translation="MITIDCREVEHLKHEMAVFVSDWVGAIPSMKLHEFILSPLGNEL
                     LEMDRTVKAIKEFFASIGETSNYGVLAKNNMIMIKSLHEKPIKKVKQTESMFACTHCG
                     YVTQYEVLWQNHMKMHYI"
     gene            14805..15404
                     /locus_tag="VGA92_08025"
     CDS             14805..15404
                     /locus_tag="VGA92_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846390.1"
                     /translation="MASKLILCTVLLGFFVILTSSSQSFAVEEDEIKATDEIKSNPAL
                     MNILKKIELSKKILTDMQEKKKIQDQKALHVQEARKVAQQDLTAELDRMNKDNEPFTP
                     VNAFARFVSKKPTNVHDIYWSMFNYQQEKIKAAKESRDRILANGGTWSEAWDSYQKIS
                     ATNRIKIIELNKDFNIRYGNVDANIQNTFDINGKLPRYD"
     gene            complement(15413..16696)
                     /locus_tag="VGA92_08030"
     CDS             complement(15413..16696)
                     /locus_tag="VGA92_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9846391.1"
                     /translation="MEPISKKWVWFIFPINIAAEGLHTAVPLFVIHLGGGIAEIAVIT
                     AMHYGVAALGSIFWGKILDKYHAKKLVLLIAFSITLVCCVWLFYTTTIEPIYAISPIS
                     GFFLVARSQVTQMLVMETSQNNQWSRLFARTSILATLGSLAAMVIGAIWSLYFDSRQY
                     FLICAISTGIAILISLQITKTNFHIERSTIAESIHGIHYIFSHFRWHHHLIFPKVPEL
                     YDYRHILTILKGKVSHEIGFLFLTNFLFYFGSNVYFTALTPFLKNLGLSDSTVFSLYL
                     LQACTMVGFFFTAPKIIAKIGEEHAAMGAYIPRLSGVLIASFLITAFVGTGSLVFAII
                     SMCLMVVGFSIYNTANSVLLFKAIPKGFEGTYLGVNSSMVGFGVFGGALTAGFITNIF
                     GYQTTFFGASLVLFTSLVLFRLYLRHRLSGKTDIK"
     gene            complement(16789..17346)
                     /locus_tag="VGA92_08035"
     CDS             complement(16789..17346)
                     /locus_tag="VGA92_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846392.1"
                     /translation="MQKFALFFLFAVTISLAAPLEFVLAQEMSEDEMMDEEVMDDTMT
                     EETMMEDEVTAETTMDEPVASPLKQLAMGVDPHEIQCASGQILVFKSSNWRPACVSES
                     SFDILLERGWISSHDPSHEDLDKMVNDYMASQPEIIEEPEGESAIEIEEDVTMEGETD
                     IDGGNMTEPQSHTIELREDMEMGAQ"
     gene            17424..17810
                     /locus_tag="VGA92_08040"
     CDS             17424..17810
                     /locus_tag="VGA92_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846393.1"
                     /translation="MKSDSETFGIEKGYGKQAIEWLNDEAKQNNLNFEARLYGYEVTT
                     QNFGSFELFSWIGDMQVARKLVTKVSKRFKIKVIEGGYKTKEKIVKFKKQDYGMVRKG
                     DKVIGHLEFSSSRFGGNWQIEKEELR"
     gene            17832..18728
                     /locus_tag="VGA92_08045"
     CDS             17832..18728
                     /locus_tag="VGA92_08045"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9846394.1"
                     /translation="MRKISDMRVGIVGTGLLGNAVGLNILKKGYVLTAYNRTKSKTKQ
                     LLESGAKIVDSPKEVAENSDIVFTIVKNADAVRKVAFGDSCIACGKHDGLIVADMSTI
                     NPIDSKDIAKEFQKHKIPFLDVPVMGGPNVAITGELVMMVGGNKETFERCKEIFDVIA
                     NKIFYLGENGTAHAVKLAMNLQIAMLALALSEGITLARGASIDPEIFLKILNSTYFKT
                     GMSESKAYKMIKDSFEPTFTLSNLKKDLDTINQAAEAFGLSLPMAKKANEVYRMAEDA
                     GFGGIDYTGILAYLKEHAEKSS"
     gene            complement(18720..18842)
                     /locus_tag="VGA92_08050"
     CDS             complement(18720..18842)
                     /locus_tag="VGA92_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846395.1"
                     /translation="MAEVQIVPFISWAVVSAVLFAVVAIVYKTYLKKKKSNQSA"
     gene            18894..19100
                     /locus_tag="VGA92_08055"
     CDS             18894..19100
                     /locus_tag="VGA92_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846396.1"
                     /translation="MPEIICQDCGKKMFHENEETVKIEEAVHKKFCRKKPGEAYSFMH
                     RDPQHTSTFDAERASDQKGKPILK"
     gene            19102..19590
                     /locus_tag="VGA92_08060"
     CDS             19102..19590
                     /locus_tag="VGA92_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9846397.1"
                     /translation="MSVNNILNQATIEFNLGNFKKALALFEQVLQLEPRHSNALIKKG
                     NILGKFGKYDEAIICYDKILEMEPQNALALVNKGLALHYLEKYDSAISCYNTILQTNP
                     NNTIALYNKASSLIRQDRIGEGLELLKETIKLDYSYKYKARFDIDFAHLQKNNDFKRI
                     VL"
     gene            19638..20882
                     /locus_tag="VGA92_08065"
     CDS             19638..20882
                     /locus_tag="VGA92_08065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9846398.1"
                     /translation="MANELNFDVVVVGAGPAGSSAAHSASKNGMNVALLEKEESVAQT
                     VRTSGVTWIDSIEEFGIPEDRYNPIKNYTFCSPNNEITIGSDKPRAAVLDVRKTYRWL
                     AEQAQKSGAELFVNTMVTDAAKTTSGISLKTISSKEETVFNAKIIIDASGFQSVVAKS
                     LGFVSQWKRFGVGAEYEAQVENVDSSTWWLMVGQQYSPAGYAWIFPLGKNIVRIGVGV
                     GKPESAVDPTERLKDLVEKRVGPIAKLGRITPLEFHYGLIPNDGLSRKTVYDNLILVG
                     DSAGQANPLVLEGIRYAIRFGRLAGQIAAKAISNDNTSEKALLPYEETWKKAILTKIN
                     SASKVQTRWIGLSDQEWDEELNIIKELSVDEFLDFIRADFGLASIVRLATHHPKLAVR
                     QLFNIVKSAAKRPSQHEKSLTP"
     gene            complement(20920..21135)
                     /locus_tag="VGA92_08070"
     CDS             complement(20920..21135)
                     /locus_tag="VGA92_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846399.1"
                     /translation="MPDESCRNCGGELKKCTVCAECRDAVSMICVQCGTRTAEQVHAL
                     CFMPEIPKIEEPTILPTGIFQKQILVA"
     gene            complement(21201..23678)
                     /locus_tag="VGA92_08075"
     CDS             complement(21201..23678)
                     /locus_tag="VGA92_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9846400.1"
                     /translation="MKLVLIFLMCSFMIGTSFAEKGTFVDKVQFIQYLEESTALEEVK
                     KGNLDIYYSRIPTELLQEQESLQNLQVFYTTGGSYSVLVNPAVGEKFNPFSFQQIRFA
                     LNYLIDRKLIVDELMGGNGVVMTSNYGPFDPDYLLIVDELEKFHFRYNPELANDMMTK
                     ILAENGAKKIDGKWLYDDKPIEITIFIRNDDPVRKSIGEVISAELEKIGFVVKKDFGD
                     LNKAFVVVYGSNPAELKWSMYTEGYAGRSAFVKYDPLGLAQMYAPWFSNMPGFNNPTY
                     WNYENQKIDDLTQRIYSGNFTSAEQRAELIGAATKEGIGDSVRIFLASKVDPFVANKN
                     VNGVINDFGGGITTRFTPINARTDDGELKIGVKQIYQGAWNPIAGLGDAYSKNIWDTL
                     YDPGVFKNPYSGKNFAVREDWKVETAGPSGALDVPTNVMNWDSDLQKWVMVEPNTKAI
                     SKITFDLMLGNWHHGQKMDLNDVLYSTYFTQEWGSQSKENDKTFDPDFSPSASQVAQT
                     LVGIRSINEHTVEVYVNYWHFDESEIASWSGMWVAMPWEIMYAMEQAVLDGEASFSRT
                     DSQAKSLNWLSLIIPRDATIIRQNLEDFLASKKTPVALSVLENPQQYYDARYQSAINW
                     IEEKNHAVISNGPFYLEGYSPDARTITIRAFDDPSYPFVAGYWSEFEHVNLPKITKID
                     VPDEIVMGTTVEIPIQTTDASQIHYFINNAAGDQVDSGILLIQDDKTRITLSDQLTNN
                     LGLGGNDLKMYAISDSVLRPDIYTTSFLAVASDGGEISETVVTQNTEKTQKTENVGVF
                     SIIIGVIIIGIIMYVRKSRKANAKADH"
     gene            23789..24424
                     /locus_tag="VGA92_08080"
     CDS             23789..24424
                     /locus_tag="VGA92_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846401.1"
                     /translation="MNQKLWLNGIDGIIPGDISVQDFAIILNSDQETAWKMLQELSQH
                     DIGKFDGETSEFVEGDKLRATILALKKGVLVDEAAKHLGWKDFEGLTAQILELKDFTT
                     LRNLVLTKPRMEIDVVGIKFGVAILIDCKHWKKHTQASLEQAVRKQIERTKHYVAKTG
                     AIAAPAIITLYQDTLSFINKVPIVPIFQFASFVDEFYGNLDDVEIIEKDSQ"
     gene            complement(24402..26141)
                     /locus_tag="VGA92_08085"
     CDS             complement(24402..26141)
                     /locus_tag="VGA92_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HEX9846402.1"
                     /translation="MTESHDDVTNIHRRYSLTLLNPTSRYHSLIISIIAAAIASFVIL
                     FVYLGKSDEILFRLPAAIGVLAVTQYIDSRFIKNKEYSKSLHMSLFGNLLWLAIILSG
                     AAASTILSKDMSLFYVTEAMMIFASFRIGIMTTTLGVSLKKALTLCFLQPLALYLVLV
                     PADLWASSLGDQLALGIGATFIVIASTWSSVTDRTGRPGVESTHKLLQAYLASISKRN
                     FVEVESIFESKSKPLTIFTSQIRLRSKDNDFRMVLPEVHPGPYHPIGGSNITYRIFKT
                     MNSCAMVMHSVSDHALNLPSQNEVENYLQSLSNYSVIKNGTICTEPVIVQINKARVTG
                     LLFDKTAVLFLSLSPHGMEDVPNYIKKEIEQFASNRNYEKVLVVDCHNAMGKDISQPD
                     SQDMLKAAKATLETLITKESHPLEFGYANSDNMKLDVMDLGPGGIGVLCLKINNLKYF
                     LGWADSNNMENGLRERVVDHFSKNGMSLLEMCTSDTHYSSTHVRTRTGYYPFGKITKF
                     DDIEGWYFKIAQDAEKNLTPASFEVMEQKAEIKVMGSAIYEEVSQALDKSLAITKAFM
                     VGSFALFIASLFL"
     gene            complement(26151..26318)
                     /locus_tag="VGA92_08090"
     CDS             complement(26151..26318)
                     /locus_tag="VGA92_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HEX9846403.1"
                     /translation="MSSKKKGAPLPASSAGLLRFFEDETKGYRLDPRIVVSIPIGLII
                     VSWMLDLFLIK"
     gene            complement(26358..26705)
                     /locus_tag="VGA92_08095"
     CDS             complement(26358..26705)
                     /locus_tag="VGA92_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HEX9846404.1"
                     /translation="MSQELRKKAVEMLLKGATLVAEPCPYCKGVRVMKDGNALCINCG
                     KTPDKQKLDEIKLGSEKQATPDQKTSSFVGSLNKKLEQLTKELESENDYEKQQQILKS
                     INSLIETIQKLQK"
     gene            26801..27109
                     /gene="yciH"
                     /locus_tag="VGA92_08100"
     CDS             26801..27109
                     /gene="yciH"
                     /locus_tag="VGA92_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HEX9846405.1"
                     /translation="MAVICNICGLPEDLCACGDLNKDSKKIVVRLEERRFKKKGTMIE
                     GLDTKLNDVHGTLSELKRRYACGGTAKEDYIFLQGDHRETMKETLVKLGFSESSIEIH
                     "
     gene            27112..28083
                     /locus_tag="VGA92_08105"
     CDS             27112..28083
                     /locus_tag="VGA92_08105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX9846406.1"
                     /translation="MQPYKLFQGIAIPYSALVSVIFGLMIFSFPIGAYLFFNSHLGKS
                     IDYHYPLSELEFVQHFGLKSLAAIKIGDLFVVAWVFFLILFAIAAFGPKKSFLKVLSP
                     IMAGTHESQDGNYLVHTIKWFSIIIILSEVINTMQEFVGIKTTLPPFENDLLQFLSVT
                     VAPVIEEIGFRIILIGIPLFLFYSHKASAKHFFKSLWNPFANLPISNSKKPIVIIVIV
                     GVFFGAAHIITDQWSEGKFAQATMSGIIIGWVYYRYGFVASVLIHWATNYMIFSYGYL
                     VSTVNETSFVDAFSHSLLQTIEILFLVTGALSIALLSLEYQKKKLEV"
     gene            complement(28072..28983)
                     /locus_tag="VGA92_08110"
     CDS             complement(28072..28983)
                     /locus_tag="VGA92_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HEX9846407.1"
                     /translation="MVKTVGKKDRCPRCGQGSVVTDTNTGENFCGKCGYVLNEENVEA
                     GPEWRSFSKEEGENRTRTGTPTSLAMHDMGLATIIGQADKDATGKPLSSTMRSTIERL
                     RTWDSRSQVHEPVDRNFRQAFSELDRLKDKLAVGDAVIEKAAYIYRKALEKGLVRGRS
                     ISALIASALYAACRDTQTPRTLKDIGNASNIKRKDIARCYRLLLRELSLKMPVVDPIK
                     CVARIASKAELSEKTKREAVRILKTAEENKISAGKDPMGLAAAALYVACVTNGENKTQ
                     RDVAEAAGVTEVTIRNRYKGLKIALNL"
     gene            complement(29086..30366)
                     /locus_tag="VGA92_08115"
     CDS             complement(29086..30366)
                     /locus_tag="VGA92_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846408.1"
                     /translation="MKNRASLLAFTLLSILVVGTVCPAWAAQLNWDAKTFETAQNPKF
                     VFQRSVFIEYPADSDIADALRGNDISIFVSADSSTTNLQELIDELNQSLSQKQSSAHV
                     TAVKIDYSASMFARDNSVSIDYRVILTPIIDGFLIREYSEGSPALFDLTWRGLKVDGP
                     ITISTEEYGQVEVNQPLSFLTKNFPNVASSIAGTEAEDILTMSLIDASGIGNQPLTNW
                     HFLFDPTGVSAETSKYGFSGAKVVVSSFTMGESSFREGIVSEKEFEATFTTDATYHVR
                     TVQSGDSANVFLAGYASPDQLQGHEVVGVSATAPTTAAQTSTGSFPVFIIYGMAGMAA
                     AGAGGFFYWSNKKAKQETEYIQRGIDPKHLRASSTSEASGGYHTLRGEAELAADSGYE
                     QHQSVYDQQGQSLPKEEPKSDSSRGSMPKGWKPS"
     gene            30540..31460
                     /gene="cofD"
                     /locus_tag="VGA92_08120"
     CDS             30540..31460
                     /gene="cofD"
                     /locus_tag="VGA92_08120"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX9846409.1"
                     /translation="MITILAGGTGSVKLVRGVISQSRDVNVVCNVGDNYWLYGMFVCP
                     DIDTIVYGLADILDYDKGWGIRKDTFGFLRQMEIFGEETWFRIGDRDVATHLVRTNML
                     KNGKNLADITKWMCEKFAVEAKVMPVTDNTIETRITTNKGEMHLQEFWVKYKGKDQVV
                     GIQYIGADKARPNPEAINAIHDSELVIIAPGNPLTSIGPMLQIKGVRKELSKNRKKVV
                     AISPLVSSKAFSGPAAEYMQAAGIEVSPYGIAQMYSDVCSNIVIDSKDRLLTKKIQNL
                     DMKVYDTNIKMNNKIAEDALASFVLKNVRV"
     gene            31462..32094
                     /gene="cofC"
                     /locus_tag="VGA92_08125"
     CDS             31462..32094
                     /gene="cofC"
                     /locus_tag="VGA92_08125"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX9846410.1"
                     /translation="MQVAAIIPVKRFSNAKTRLGLPQEKTELFCKIMLDEVLSTVSQS
                     QKINKIILVSNDESALEMGKKHNTVSIFEEKERGVNAAVALAEKYLLENGFETSVVFP
                     QDIPLMQPVDIDNLFAFQKNTKSLLIVPSRKFDGTNALVRTPVDIIETHYDEDSYKIH
                     LSTGKLRGIPTSFVLIHRIMWDVDDRSDIGYVMNSIEKPQIGDKLRKILE"
     gene            32110..32829
                     /locus_tag="VGA92_08130"
     CDS             32110..32829
                     /locus_tag="VGA92_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846411.1"
                     /translation="MLSIALLVAFPGGASLIPGGSLTFYVHDDDLNTNHRGIDEITTA
                     GLLEFTINGALISGPAKIIETSGNSGVFVGKISIPTSVNGKHLKQGDTLVIKYNDESD
                     DSGNPNTVARSISIAKAITDFDTSPSKIRIGQTFQVKIYDPDFNFDSREADNIPLRLV
                     EFRAANGIRATLIDDAFNAKPKSLRETGDNTNLFVVTIKMPKEIDGKKLKVGSTAELR
                     FTDTTSPSGTFEILKKTVRIG"
     gene            complement(32843..33122)
                     /gene="rnpB"
                     /locus_tag="VGA92_08135"
     ncRNA           complement(32843..33122)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGA92_08135"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            complement(33228..33731)
                     /locus_tag="VGA92_08140"
     CDS             complement(33228..33731)
                     /locus_tag="VGA92_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HEX9846412.1"
                     /translation="MNVQVLMFRQDDPKKCTAAKLVKFGLAKSIQRTQTDTIVLDPFA
                     KQTLLRKDALIAHSITGIDCSWNRADNAFAKKFVGIPRKLPPLLAGNPVNYSKLNKLT
                     TVEAIAAAVFILGYEDMTMELLGKFNWGHTFYELNEHLLKDYSKMTSEEEIEPLLREY
                     GLVKNTG"
     gene            complement(33764..>35540)
                     /locus_tag="VGA92_08145"
     CDS             complement(33764..>35540)
                     /locus_tag="VGA92_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846413.1"
                     /translation="YEYGEKLTIIGHVIPEARSTFVTTPVSVSIQDESGKPLTIVAKD
                     KKLKIRDDSLIVAYSFTAIPDTAGYYKIDMPLNLSTFTPGAYIIEAKYGKSVTSTIFS
                     VSGTLDTTDKNINAKLDKSIYGLGETVSLQGTLLSGQSAVKIILTTPAGKTVNSGAKV
                     DNSKFSWSWTVPSKDYDLADIRDPREARPSVFGNYKLTISATSETIGLFFKVSKNPET
                     DTLEIKPLAVATDKDVYKAGEKLTVLGNAIKRQQGTGLGSGVIPDRVGIQVKTLANKQ
                     IYDGMVSFDNAGNFKATYDLPLTIFKDGTYKVTATYQKLITDTRFEVRNDIPFGGDGK
                     ISITLNTDKEEYSPGDTVQIYGATNKILSLGKLDLVVIPEESKINCGTSNCGLGGKKI
                     DLTRYYGNGLYKYDYKLPSSLDFGTYVIKVNAEFGTFSKTFKVIEKKEPQKTVSPLGK
                     ISDKFNRITDTMIDIPIFEQNVQDQDVVPWLIQGSVITTRGSEKSVNLKVMADDGSCV
                     IGQDEGCLVSKSTKTSEGGYEVVSFAGLDYKVTYSGPEPFVENFSITPESEDGVIPNS
                     IWTVEIIKENQSSRFYYEIIYKPIQ"
BASE COUNT        10460 a         7205 c         7106 g        10769 t
ORIGIN      
        1 gtcaagaatg ttgcttttgc ctgatccgtt tgggccagaa atagaaacaa gcccgggctc
       61 aaaattcacc gtagtatttg taaatccaaa tgatttgaag ccaaaaatct ccaccttttt
      121 gatatgaacc aattataccc ctttgagatc tggtttgtta atcaaatatt gactaaaatt
      181 gttgactaga caaacaattg ttataatgct tttatttttg ttgtactagt cacactaggt
      241 taactaatta agtcagaata aaaacaagaa ctgttggtca gactaggcat tctgcagaca
      301 cgctcgtacc ctacaaacca aaaaggaatt gacgaagttt caaaaatgct gataggggca
      361 gcaaagtcag aaaccgacat catctgcctt cccgaacaat ggcaaaagga aaacaagttt
      421 gttgattttg atgtagagtt tgcccagctt aaaaaaatag caagagaata cgcaatgacc
      481 ataattccag gcgcatttta ccaaaaaaag aaaaaaggat tcgtgatttc tgctccagta
      541 attgatagtt ctggggatat tataggcacg caggaaaaaa tacacccttt tgattatgaa
      601 aagagcttaa tcagagcagg aaccaaggcc cgtgtattta agaccgcctg caagtttgga
      661 attataattt gctacgacat ggtgttttca gatgtggcag aatcacttgt aaaaaaaggt
      721 gcccaagtcc tgttctcacc ctcacgaatt gtaaagcgag gaataatccc atggcacatg
      781 tatgtgcaag ttagggcact agagaataga attccaattt tggctacaaa cattcagaac
      841 aatcggtttg gcggaaatgg catcatagtt gacctaaccg aagatgataa cgttatgata
      901 ccaaaaatca caacattgca tggtcagacg acaaaatcat cgtcatttga tttatcaaaa
      961 tacgaaaaaa gcagaaaaca cagatatttg gaccacagaa ggtttgcgta aagggtgttc
     1021 aattttataa ccccagccat ttttgagaaa acatgataga gccgggaaaa ccagtccaag
     1081 acattaacat caccaatcaa acaaatattg aagagatttt tgcgatgatg tccaagtctg
     1141 gagggtttga gtcaagaaat cttgcagacg gtcttgaaat tctttcttcg atgattaccg
     1201 ataagaattg tcttaggttt ttgtcatttg ttgccgcaat aacatcgact ggaactcgtg
     1261 gcatcataaa agacatgata caaaaaaaat ggtttgatgt agtaattact acttgtgggg
     1321 cattagacca cgatattgcc agacattttt caaactatag ggagggatct tttaccatgg
     1381 acgattctca gcttgcagat caaaacatcc acaggcttgg aaatgttttg gtcccaatgg
     1441 aaagctacgg tcctctaatc gaggaaaaag tgcaagcaat tcttgaagaa gcatacagtc
     1501 aaggaaaaaa agaaatgtca acagctgaca tcacccatga gattgggaaa agacttggag
     1561 aggactcttt tctttattgg gcttacaaaa acaacatccc agtcatagtt ccaggcataa
     1621 tggatggagc ggtagggagc caggtttgga tgttttcgca aaaacaccgc gactttaagc
     1681 ttaacatgac tgcagatgcc gatatcatat caggacttgt ctttaaggcg caaaaatctg
     1741 gcgccttgat gcttggcggt ggaatttcaa agcatcacac gttatggtgg aatcagtacc
     1801 gagatgggct tgattatgca gtatacatta ctactgctca agaatttgat gggagtctta
     1861 gcggagcgct tgtacgcgag gcaatctcct ggggcaaggt aacgcagggt gccaagcaga
     1921 ctaccataca tgcagaaata acgacaactc tgccgttttt gtatagagcg ttgttatcaa
     1981 aaatcaaaca ataatttgat tatttcataa aagtatatca gtgattttga gttgggtatt
     2041 gtatgttacc tcgtctctgg gtcaaagagc taaaacaaat cgatattgag gggggttttc
     2101 taatagatgg ttttccctca atcgggttca ccagtgcaat cgcaagcgaa tcattgatgc
     2161 acacatctca gtacgaccta gtaggtgtta ttgattcaat tgattttcct acagtaacaa
     2221 tcctaaaaga tgccattcca aactacgcca cgagaattta tgtcaactcc gatctcaaag
     2281 tcagcatttt tacttcgtat cttaccatta atgagccgta tcatcgagca attgcaaaaa
     2341 tgatgttgag ttgggcaaaa aaacacaagt gctcactagt cgtaagcagt tccccaatga
     2401 agttcccaat agaccggcca gacgagataa ttgcagccgg aagcaccgat tccgcacgtg
     2461 aaacactcaa gaaagccggc atgacactat tacaaagtgg aaccatccca gggatacccg
     2521 gggcactctt aaacgacgga atgctaaacg gtcaagacgt catagttgtt ttagttaacg
     2581 tcgatgaaat ggggcctgac tttaaatcga gtgcgcactt gtgcatggca atgtcgaagc
     2641 ttttacctgg ggtgtcttgt gatttaacca tgatacaaaa acaagcagag attgcagaaa
     2701 agcaaattaa agaaacagaa aaagaaacaa gaaccttaaa agaatcaatg tatcgataga
     2761 ctatctaaac atcaatgcaa gagttttttg ggttttttgc ggcagtcgta gttatttcgg
     2821 cttcaggagt aatggcacca ggaccacttt ttgctgcaac aatcgcaagt gggataaagg
     2881 aaggaaaact cgctggactc aaggtggcag ttggtcatac ggtcgtagaa tttctacttg
     2941 tgattctaat tgggatcggc gtcttatcat tagaggtact gccagaattt cgcgtagtaa
     3001 ttggagtttt gggagccctt agcatttttg cttttgctag cttgcagctg cactccacat
     3061 taaaaaaaca gggacatgta caaaaagaat ccaaatatag ttccttcctt acaggaattt
     3121 tacttacagg attaaatccg ttttttctca tttggtggtt tacaattggg tttaagctta
     3181 ttgctgatgc gttttcactg tggtcgtttt tgggaatttt gataatgttt ggattacaca
     3241 tttggatgga ttatgtatgg cttttttttg tatcagcatt atcatcaaag ggaaccaagt
     3301 tcttaacaaa caaaggatac aagttttgta tgattgcaat aaatgtagtt ttgatttatt
     3361 ttgggatttc ttttatcaca aacttgagtt aaccgcagtc aggtgtttca aattcggatt
     3421 tgcattcatt atttaggtca cctatttttt ccgcaagcga gatgcaaatg ttaggatcca
     3481 tcgttttttc atattctatt atttcatcaa taattgaaaa ttgttttgcg gaacacgagt
     3541 gaaagttctg aaaaaacagt aatgtagaaa ttataacaat tccgcataca acagaaactt
     3601 tgtagtagtt atgcctttgc aatatcaatt tctatcgtag aaacgtttct agtcttaccg
     3661 tcctgagaca gtaatgagtc agaggcgatt cgaatatcct ttattatgta gcctacggta
     3721 ttcattcttt ttacaatcat ctgagagaca tcaactgcct gtgtaattct ttgcccacgc
     3781 gcttttattg tcacgacttg ttggtttgaa agctgcgtaa gtgtggcagt aacataagtc
     3841 ataattggtt ttataccaac aaaaatcaca tctcgtgtct tttttgcact gtcttcatct
     3901 aaaacatttt cagtttcttg tggcgctggt gtgttttcag tttcttgtgg cgctggtgtg
     3961 ttttcagttt cttgtactaa agttgttgtt tctgtttgtt cgtccttagg ttctttgatt
     4021 ttttgagatt ctgcaaatat ttcttctgca tctgttggtt tgtcttgttc actcataact
     4081 ttatcctata gattgttctt gcttttctgc gtttatttta atgtttgaga ttttgttttg
     4141 caagataatt ttcgattgcc ttttcaattt cgtctggact tttacaagtt gttatatttt
     4201 caacaatttc tttttcttcg atctcatagc aattcagaaa ttcttgtgaa tctttgaaaa
     4261 ctagaagaac ctttccgttg tgaagacaat agtacatttt ttcttgtttc attagttcag
     4321 ggattatttt gattcctccc tcatcagatg ttattgggta cgacacagat tttcaaccac
     4381 accaagatat ttagaccctt catatgcgat attgtaataa agattttttt cgtatactaa
     4441 tcgatgtcag aacggattgt ttttcatata gattttgatt atttttatgc acagtgtgaa
     4501 gaaatccggg accagactct gaagacaaag ccagtctgca tatgtatttt ttccgacaga
     4561 ggtggcgata gcggcgcaat agctactgca aattatatcg caagaaaata tggcgtcaaa
     4621 tcaggaatgc caataagatt tgcaaaatca aggctgcagc aaattcctga tgccgtgttt
     4681 cttggagccg attttccttt ctactcggat atttctgaga aagccatgga agtaatcaag
     4741 agttatgcag atgtttttga gtatgtagga aaggatgagg cgtatttgga cgtaacaaga
     4801 aaatctgaaa aaagctttca gattgccact cacctagcac agcaactcaa aaaccagatt
     4861 cgaaacgaga tcaaaatgac ctgctcagtt ggcatttctg caaacaagct tgtctcaaag
     4921 attgcctcaa gttttagaaa acccgatggg ttgaccgtag ttgaaccgca caagacggaa
     4981 tcgtttctca atcccctcaa gattagcgat atcccgggag taggcaagat aactgaagaa
     5041 aaatttactg aaatgaacct aaagacgatt gaggatttga aaaatgtaga tgtctttacg
     5101 ctaaacaaga tgtttggccg caagatttca tcatatcttt acaatgcagc aagaggaata
     5161 gatgaagaac cagtagcaga aagagcccca tcaatccaat acagtagaat agttacacta
     5221 aaagaggatt ccaaagattt tgaatttttg gcaaaaagtc ttgatgagat atgtgatgat
     5281 cttcatcaca ccatactaaa acacaaaaaa atgttcaaat cagttgggat ccagtttgtc
     5341 cagtctgatc tatcaaacaa aactaaatct aaaatgctca aaaacccaac attttccatt
     5401 gaggagctca aaaaaacggc cttgctgctc ttagaagagg ccctagttga tcagaccatg
     5461 aatgtaagaa gacttggtgc taaggtttcc gaattgtcag attttgaggg tcaaagctca
     5521 attacaaact tcttttgagt taggcgcttt gttttttgag tcgttttgtt tctattgcca
     5581 ttaccaaaaa aagataggca aaaagccacg gtaaaaacat tatttcgccg gcaattccat
     5641 gaaattcttc aaaatctgtc gggtttgcag aaaccttgag aacaaaaatt gatagtgaaa
     5701 atattctgat catatttacg cctattgtgc ccaatattcc aataacaaag tatactgcct
     5761 tgcgctttgg tggaatattc atcttgaaca aaaatgccat cattactaaa gaatagatga
     5821 tcacactgtg tacgcctgca gacggccaga agacttgcag cgccatagag ccgtgctccc
     5881 ctctaagaaa catcaggttg tcacgtgcga ttgcagtgcc aagatctaat gcattaatta
     5941 ttgagacgtt tgcctttacc aagtgcggta ctatgtattg tagcgggcca agtgtgtcat
     6001 agggaaagaa cgcgtcaagt gaaagtatta ttgcacttcc acaaagaaag attggcccag
     6061 ccggggcaat tcttatccag cgtcgtccaa acagaatgca tattgctgcc atcacaaacg
     6121 cacccatcac tacaaagtcc cacatccatg tccacgaata aacaagattc acgttgtatt
     6181 gttctgcacc attaatgatg tagtccctta gcccatactc taaagacaca aggtacacga
     6241 ttactgccgc tgcgagcggg attattgcaa ttagtcgttt ttttgaaatt agtatcttta
     6301 ttcctatgag ctcagcaaca acaaatgcca ttgcaaaaat aaatccccct cggccttggt
     6361 tccagctaag actaaacgaa tcaggataag cagctagcgc aaacaaaaca gggctcacaa
     6421 ttaccaaaat tgcaagcatt agcgttttat tttgcattac agggggtaaa tttgcaataa
     6481 ataaaaacta agagaggtta ttttttctta atcgtaagcg ttttttgaat ctcgctcata
     6541 gtgctgatga atttttgctt tgcttcatac tcgtttgaga ctttgactat gatttccgat
     6601 gaggaaagca tgacatcttt taccactagt ggcgcactga ggcccaaaag ctcacttaga
     6661 aactcgaaga ggttgtggaa ttttgttgcg tttctgtact ggaattggat gcggccatcc
     6721 ttgaaggtag gctttggatc aattgggacg accagatccg gggaggtcat ttcggtgatg
     6781 aatttgtttg tgttattttt tattgcgttt gagagctcgt ctcgttttgc ctccaagttt
     6841 gcctttgatt tttgtaatgt atcaagctga tcatcatatt caaccccaat catttttgca
     6901 aaggtagatt cggttggttt tttcagcgga agctcgtgaa gtttcatgtt tactaccttt
     6961 agctcttctt cgatttgttc tagatagttg cggtttgtct tgatttgtcg cgctaattca
     7021 tcaagatagt taaaatcaac catattgacc actagctgaa ttgatgtaca acatccatta
     7081 gtgattgctt aaagtcctca gtcgtaaggc cccacttgcc atattcttct ttgtatgcat
     7141 cccaagactc ttctgcctct ctggcaatct caagtatctt tggtccaatt gcctttgtat
     7201 ttacaaaaat tgtgatgttt gcctgctctc tgtccattat tggaatcttg acaaatatca
     7261 tcctggaatc ttcgatgttg aatcgttctt tgatggtgcg tttgagtatt tcgcgctctt
     7321 tgatgtcaaa gtctcccttt actttcacta gagcacaaca tgaatctcgc ggtccgccat
     7381 aatttctcac atcattctca tctatgtcaa agagtaacgc atcctgtttt ttgttaaggt
     7441 tttcaattat tgcatccatt gtgttatttt ttccaccagt tatttggtta agaccccact
     7501 ggtcatcttc tggtagcact ggtattaccc atggtattgc catctttggt ctctttgtcg
     7561 caagccatgt cctatagttt gacatgtccc actcggtttc cctggcaaat gacgcaataa
     7621 acatcgagat tgcgtttgcg gcaatcaaat tcattccctt gtagtccttc caacctacct
     7681 cgtgttcttt tggcaaatca tcaattattg acgggatttt ttgaactcgt ttctcatact
     7741 tgtgtccaag ctcgcgcagc cttttgtttg agaataaaat ggagcagtcg ctgtacttga
     7801 tttggtattc aaggttcatt acaatgttta ttgccgcagc ctcaagaatc actggatccc
     7861 atccaaattc tggcaatagt cccagtgttg caattgtcgc gtgttctttt gtattgcttc
     7921 tgataaattc aagaaatgag caggcaaatg agctcccagt tcccccaccc attgcaaatg
     7981 ggactgtgta tcctcgtact ggttcaggag ataatgccgc tagctgtttt gtaaagtccc
     8041 atttttgggt gatctcgttt ttgaatctag agcgtccttc ggcccagttt cttgcggcgc
     8101 ctcccgctcc atggattacg tgcttgtcac gtagtgcgaa aagatccgga tatgattgta
     8161 aaatgaggtt tgctgccctt gggtccaaat caacaagcaa tgcccttgga acgagtggag
     8221 tttcaccgcc ggtatatgct ccaggaaaac gcaaaattgt actaccgtat ctttttagaa
     8281 tccttgactc ctccttggaa tctttgagga ctggttttag tgggtctgca cctacatcca
     8341 aaagtaatct tctgtaggat tccgcaccaa gacctatgcc acagtgacca aaacaggtca
     8401 tcagaaccgg cgctggcggt agtggtgccg tagttgttgc tgccatgatt agatacctac
     8461 ctttttgact ttaactgcac agcgtaacaa aagtggagta cctacttgag ccttgtagac
     8521 ttgtgcgcaa ttcatgctca gaggatcgta ggaatggatt taagacttga gtagaaatta
     8581 tactgagact atgtagaatg ttatcttgca tgattagttt tttagttaaa ggttagttat
     8641 tcgtcggatt cttcagacca gtcttcttcg tcgggcatag gcttctttga ttcacagcta
     8701 tcaaagacgt tgtttccctg gccaccatga catcggtcgg tgtcgctgcc tccgtttagt
     8761 gtgtcatttc cttgtccacc gtgaatcctg tcgtttccct catcgccgtt tagtgtgtcg
     8821 tttcctggtc taccaaagat cttgtcgttt cccggaccgc caaacaccat atcattgcca
     8881 attcccgctc caagccagtc atttccttcc ccaccaaata ttgtgtcatt gccttggttt
     8941 ccgtgtactc tgtcattacc atctcctgca tcaatgaagt cgtttccttg tccaccccag
     9001 atcacatcat ttccgtcatc tccacttaac acatcatctc cttgttgtcc gtgaatcttg
     9061 tcattgccac ccatcccaaa gattttgtcg tcacctttgc ctccaaagag gttatctttg
     9121 ccggtgccgc caatgataca gtcattacca tcttttgcag agaccttgtt acctgcatct
     9181 gatgtcaata tcaaatcatt tccttttgta cctttgatgt ttttgcccaa gtgtccgtca
     9241 cgattgtcaa ttacgttgta agtaccgatt gcaatcagct gatcaattgt catcccatca
     9301 cagaagggat cccctatagg tggaattgga atacattctg tggcccctgc ttccgatgtg
     9361 gttccatcag gacattgttg ttgtgatgct gcaccaaatg tatcaacaaa gaatcccgga
     9421 tcagctaaaa gacaggatga ctgacctgaa tctggttggt aagtacctgg ctcacaaagc
     9481 tctgcttgtg tagctcctaa tattgaaaca aatgttccag gtggtgctag atcacacgat
     9541 gcggaaccct ccacagaggt aaatgttcca gctggacaga gtagtgactc ggtggctgca
     9601 ggacctggcg catagcttcc tgcaggtgca ggctcacatg atgattgccc tgtcattgat
     9661 tgaaaggagc cagcagggca ttctgtctgt gatgttgctc cagtcactgc aacaaaatga
     9721 ccaatgtcag aaggaataca tgatgactga cctgaactcg gttgaaaggt gccaattgga
     9781 caaagttctg cggctgtagc gccagtatct gccacaaatg atcctggggg agcatcatca
     9841 cattgagcag agccaacatc taaagagaat gtgcctgcgg aacaaagtag tgactcggtg
     9901 gctgcaggac ctggcgcata gcttcctgca ggtgccaaaa tgcacgaggt ttgacctgct
     9961 tggtcttgat atgaacctgc aagacattct agttgagcag tagcgccaga ttctgaaaca
    10021 aagtgtcctg aatcagctag aatacacgag gtttggtttg gatctggttg gtaagtactt
    10081 ggcaaacacg gttttattgt attgtctatt tcatcatagt aagatcctgg aggtgcgaaa
    10141 ccctcgtcga ttattccgtc acagtcattg tctagattat cgtcaagctc tggagctcct
    10201 gggtatactg taacgttgtt atcattacaa tcaatatctg atgtaaatcc atccaaatca
    10261 agatcagggc atggtatgat aacgattgct ttaccactaa gggttccaga attattaacc
    10321 acgcccccac agtgattgtt gataccaccc aaattctgaa tagtaccaaa gttgttaatt
    10381 gtaccaaagt tgttgataat accaccaacg cttgtcccga acgtacgacc agtgggattt
    10441 aggagtgtca ctccagcagg tatgtcaaga gtatcaccag tactgataaa gaatcctata
    10501 tttgagttgg tacatgtcaa tgttgcagca tcccagaatc cattcaagaa ggtttgacag
    10561 gtcatagcat cattcaaaac aagagtccgt ggaagtgcaa aggcgtcatc tgcttctgtc
    10621 aaattaatca aaaaagctgt cagtacacta aacaaagcga tttgaacgca aactagtgtt
    10681 ttacttgcca aaatcctcaa cccatgccat tgaagtgatc acatggtaga ttgagaatat
    10741 ttaaggactg tggcacattt ggaatggttt gtatgcccat ttggcaaaat gtcagacatt
    10801 ttaaaagtaa tcggtaatcg atttttgttt tagcattttt agagttgagc cctttgagag
    10861 ccattcgttt ttgctgatgc tcatgttctt gcacgccaag ttcaatccta gttcaagcgt
    10921 atcaacaagt gttggtggtt ttgacattgc agctctaatt gcctcacgta cctgccacac
    10981 ccccacagga acagcgtact ctggtctaat ctctcttaaa accaaaacac ctgcttgtct
    11041 ttttttcttt ttcagatatt ctgctattcc aagctttgca gcaaaataag caccagcaat
    11101 gctcggataa tgatcaattc cgttggcatc ttcagcatcc acaccaaaac caatttgacc
    11161 agattctgta taccacgcct cttccatctc aaaaatccag tggtgtggaa aaagtagaat
    11221 cgaaaacaaa tttcccaagt gttcgtgggt aaatacgaga tacgagtcta tttgagaata
    11281 atccaaaatt tcagctacga gattttttga tatgatgtta tctgttgcag taatgctcca
    11341 gcgggttgga acaagttttc tttttttgcc aaacattcca atgctgaagc atttttggat
    11401 ttttgagatc tctattcctg aattatatag ctgcaatacg gcatcttcgg ctttgagatc
    11461 cttgtcatag tatgattgtt ctataacttt gttagaggac atgccagaga atttggcagt
    11521 ttttatttcg ccgattggtc caaatggcgc gctttcccca tcaattgtag taattggcat
    11581 ggtgtttttt tgaaatacaa tatcagaatc aggcgtgttt gatgccatcg ctaattcttg
    11641 gaggttttct attagttgac cttttggttc aaggactgaa actttttgaa ttcccctcac
    11701 caaattgagc ctaaaattga caatctcctc aaggcttttt ccaagccagc gctcaggcaa
    11761 atcaaggagg ctcgtatcac catggattgg tgggaccatt ggcccgacgc ctacctttgg
    11821 atatccaaac gagccaacaa acacagaggg aggactagaa ccactaatcg aatccgagga
    11881 aaatagattc ccatatttcg agagatatgc cttccagttt tcctcaattg atcttcgaat
    11941 atcccttgct gtaggatcca taccataatt gacgctttga tgtatttagc ctcatttgtg
    12001 gaatcattgt tatgcgaaaa tgacttaaaa ttcaagtagt tgaaaataaa ttcatggttg
    12061 caaaaagaat agtttcattt cttccaagtg ccaccgaatt actgtatgag cttggtgcag
    12121 gagatcacct agttggagtt acccatgagt gtctataccc cgaacaagca aagacaaaac
    12181 cacgagtcat aaattctgta ttcgacccgg aaaaaatgac gtcgcgccag atagaccaaa
    12241 agatctcagc gcttacaaga gagggaaaaa acatctttgt agttcaagaa gaaaatctca
    12301 aaaaggcaaa tcccgatctc ataatagcgc aaggaacttg tgctgtttgt tccgcctaca
    12361 caaatgaggt aaacaaagcg ctagcaattt tagaaaaaaa gccagacgta gaagtgctcg
    12421 atccccatac tattgaggat attttatcaa gtgtaatgat tgttgcaaaa aaaattggca
    12481 aagaatcaga agggcaaaaa cttgtagagt cattaaaaaa cagaattgat tttgttaaaa
    12541 ataaaaattt caaaatacgg tcaaagattt tatgtattga gtgggttgag ccgtttttta
    12601 cgtctggtca ctggattcca caaatggtag agattgcagg cggggctaac ctcatcagca
    12661 ccgtcggcca gcactcgcaa agaatgacag ttgacgaggt ggaaaaagct gatcctgaca
    12721 tcatagtcat gatgccatgc ggctttgaca ccaaaaggac tgcaaccgag tgtgccgcgg
    12781 ttttgcaaaa cgaccaaaga tggcagggcc tccgagcagt taaagataac aaggtttttg
    12841 cagttgatgc aaactcttac tttagcaagc caagcatcag aaccataaca ggaatcgaga
    12901 ttttggcaaa gataatacac cctgaggaat gttcagcgct ttcggttcca gatggcgcct
    12961 tcagacagat ctagttgttt tatttgtgtt cgtcatcgtt gtcttcaagc gttcttttgc
    13021 ttggtcttgt tgggacaaac aacactgtca tgacattcgc ataattgcaa acctaatagg
    13081 aagaccggat cagaaaaatt tactttttta tgatttttag ttgcttgatt atctgattgt
    13141 tacttgcatt ctagagttag tgcctgaaat taaaacgcca aaaactattt ttgaaaaagt
    13201 tagtgtgtgg atattaagaa taagtttgtt tgaagtcgac acacattctc tgatattgaa
    13261 ggttgttctt acctagtaca agaatggcct gaatcatatc tctctggtcc tatctcgtgg
    13321 cttcttgtct tcaatgattc aggcagattc ccttctatct ggccttacac tcaacagata
    13381 gggacttggc cgtggtatag tagacatatc ctaggatccc agttgaaata attcgaagtg
    13441 ggatctcata tgatgccaaa aacgcagttg caatcccgcc tgcagtgcca aatgtagaaa
    13501 ttattgcaaa tcccacaggc ccgcagctgc aagcacctgc agatgcgcca attagggagc
    13561 caagaaaacc cccgcttgct ttgcttaccc tttgtaatat ttttaccctg tacacattca
    13621 taggaattac agctgcagac aaaacagata ctgcaaggat taatgcgaac ccaagtaccc
    13681 tgtcgtctgg cacataaaaa acaaagtaag gctcaaaaaa gacatattct gacagcgtag
    13741 aaagaagaaa aaacatcacc ataaaaattg ctacagcaag cccaatgtag ccataatttg
    13801 aaaagaccag tttagtcgca ttcaattttt agctgaaccc atgtacgagt taaaaagatt
    13861 cttttacttt cgcctaaaaa gcctggcaag tttttgaatc gccgcgtcaa gtggacatgt
    13921 ttcacaagtg acctcttttg agtcttcttt gtcttccatg acacaagcca tcgcttatca
    13981 aatataaaaa aattctcaag atctccagca caacgctaaa taccaatagt ctgtgcaaaa
    14041 tctatgggtc tagtttttga caaattcatc acggcgttat ttgtaataat tcttggatcg
    14101 gcgtggtttt tgacaagacg ttacgaatca tcgcgcctgt acaaaattgt agcttatgac
    14161 gtttttggaa gtcaggtaag gctcgatggg attagaacca cgttcaacac tcattcagta
    14221 gccattagct ttgccaagca ctacagagaa atatttccac attatgattt tgcattaaaa
    14281 tctgaaatgc ccaacataaa acgcggtttt ttacccaaaa aaactcagat ataatgcatt
    14341 ttcatgtggt tttgccaaag aacctcatac tgtgtgacat aaccgcagtg ggtgcatgca
    14401 aacatggatt ctgtctgttt cactttttta atcggttttt cgtgtaatga tttaatcata
    14461 atcatgttgt tttttgccaa gaccccgtag tttgatgttt ccccaataga ggcaaaaaac
    14521 tcctttattg ccttgactgt cctatccatc tcaagcaatt catttcctag tggtgagagt
    14581 atgaattcat gtagcttcat ggaaggaatc gccccaaccc aatctgaaac aaatactgcc
    14641 atctcatgct tgaggtgttc tacctccctg cagtctattg taatcatagt tggaattcga
    14701 tcagatcgta tttttaattg tgtctttgca tatttctagc acttaagcat tgctaagaaa
    14761 tcctgtttaa ttgctaaata caaaatgttc catcagtgtt ttcgatggca agcaaactca
    14821 ttttgtgcac agtattgtta ggtttttttg taattttgac ttcaagttct caaagttttg
    14881 cagttgagga agatgaaatc aaagcaacag atgaaataaa aagtaatcca gctctcatga
    14941 acatcctaaa aaagatagaa ttatcaaaga aaattctaac cgacatgcag gaaaagaaaa
    15001 agattcagga ccaaaaggca ctacacgtac aagaagcaag aaaggttgcc caacaagatc
    15061 ttactgccga actagacaga atgaacaagg acaatgagcc gtttactcct gtcaatgcat
    15121 ttgcaaggtt tgtgtccaaa aaacccacta atgtgcatga catctactgg tccatgttca
    15181 attaccagca agaaaagata aaggctgcaa aagaatctcg tgacagaatt cttgcaaatg
    15241 gaggaacttg gagtgaggca tgggattcat atcaaaaaat ctcagctaca aacagaatca
    15301 aaataataga gctcaacaag gactttaaca taagatatgg taatgtagat gccaacatcc
    15361 aaaatacatt tgatataaac ggcaaattgc cacggtacga ctagtgttta gattatttta
    15421 tgtcagtctt gcctgaaagc ctgtgccgca gataaagcct aaagagaact agtgatgtga
    15481 acagaacaag tgaagcccca aaaaatgttg tctgatagcc aaaaatattg gttataaagc
    15541 cagcagtaag agcaccgcca aagactccaa agccaaccat tgagctattc acgccaagat
    15601 acgtcccttc aaatccttta ggtatggctt tgaataaaag aaccgagttt gccgtattat
    15661 aaattgaaaa ccccaccacc atcaaacaca ttgatatgat tgcaaaaacc aaggagcctg
    15721 ttccaacaaa tgcagttatc aaaaagctgg caattagaac tccacttagc cgaggtatgt
    15781 atgcgcccat tgcggcatgt tcctcaccta tttttgcaat aatttttggt gctgtaaaaa
    15841 agaatcccac catggtgcat gcctgtaata gatacaaaga aaataccgtc gagtcggaaa
    15901 gccccaagtt tttcaaaaaa ggagtcaggg cagtaaagta aacattgctt ccaaaataaa
    15961 acaaaaagtt tgttagaaac aaaaacccaa tttcgtgtga aacctttcct tttaggatcg
    16021 tcagaatgtg cctatagtca taaagctcag gcacctttgg aaagataagg tggtgatgcc
    16081 acctaaaatg actgaaaata taatgtatgc catgtattga ttcagctatt gtacttcgtt
    16141 caatgtgaaa gttggttttt gtaatttgaa ggctgattag tattgcaatg ccagtagata
    16201 tggcacagat caagaagtat tgtctgctat caaaatacaa actccatatg gctccaatca
    16261 ccatggcagc aagacttcca agtgtagcaa gaatcgatgt tcttgcaaac aatctgctcc
    16321 actggttgtt ttgacttgtt tccatcacaa gcatttgggt cacttgactt cgcgcaacca
    16381 aaaaaaatcc tgaaattggc gagatggcat aaattggttc tattgttgtt gtgtaaaata
    16441 gccacacgca gcaaaccaaa gttatagaaa acgctattag caacactaat ttttttgcgt
    16501 gatacttgtc cagaatctta ccccaaaaaa tcgacccaag tgcggccacg ccataatgca
    16561 ttgctgtgat aaccgcaatt tcggcaatgc caccaccaag gtggattaca aataatggaa
    16621 ctgccgtatg tagtccttct gctgcgatgt tgattgggaa gatgaaccat acccattttt
    16681 tacttattgg ctccattgtt agaattagac gtaaacccgc actaatctat aacgcattaa
    16741 aaaatcttgt acaggcttgt tgaattgttt aatcagatca tctttgaact attgtgcgcc
    16801 catttccatg tcttcgcgca gctcaatcgt atggctttgt ggctctgtca tattgccacc
    16861 atcaatatca gtttcgcctt ccattgtcac atcttcttcg atttcaattg cactttcacc
    16921 ttcaggttct tcaattattt ctggctgtga tgccatgtaa tcattgacca ttttgtcgag
    16981 gtcttcgtgt gagggatcat ggcttgaaat ccaaccacgc tcaagcagta tgtcaaagct
    17041 tgattcgctg acacacgcag gtcgccagtt gctggatttg aacacaagta tttgtccgct
    17101 agcacattgt atttcgtgtg gatcaacacc catagccaat tgttttagtg gagatgcaac
    17161 tggttcatcc atcgttgtct ctgccgtcac ttcatcttcc atcatcgtct cttccgtcat
    17221 tgtatcatcc attacttctt cgtccatcat ctcgtcttca gacatctcct gagccaagac
    17281 aaactctaga ggtgcagcca gcgagatcgt gactgcgaat aagaaaaata atgcaaattt
    17341 ctgcattaat tgatgtgatt tgacttggaa tttaaccttt ttcaaaactc tagaataaga
    17401 ggagtttgtg tacgagcaat atggtgaaaa gcgactctga aacatttgga atagaaaaag
    17461 gctatggtaa gcaagcaata gagtggttaa acgacgaagc aaagcaaaat aatctcaatt
    17521 ttgaggccag attatatggg tatgaagtca ctacccaaaa ttttggttcg tttgagcttt
    17581 tttcatggat tggtgacatg caggttgcaa gaaaactcgt aaccaaagta agcaagcgct
    17641 ttaagatcaa agtgattgaa ggcggatata agacaaaaga aaagatagtc aaattcaaaa
    17701 aacaagacta cggtatggta cgaaaagggg ataaagtgat tggccacctg gagttttcct
    17761 catcaagatt tggcggaaat tggcagatag aaaaagagga gctgcgctag agcacaacac
    17821 aaataatcat tttgcgaaaa atatcagata tgagagtcgg aatagttggc accggattac
    17881 ttggaaacgc cgtaggcctc aacatcctga aaaaagggta cgtactaaca gcgtacaata
    17941 gaacgaaaag taaaacaaaa cagcttttag aaagcggtgc aaagatagta gactcgccaa
    18001 aagaggtagc ggaaaattcc gacattgtat tcaccatagt aaagaacgca gatgcagtcc
    18061 gcaaggttgc gtttggcgac agctgtattg catgtggaaa gcacgatgga ctcatagtag
    18121 ctgatatgag tacaataaat ccaatcgact caaaagatat tgcaaaagaa tttcaaaaac
    18181 acaagatacc atttttagat gttcctgtaa tgggtgggcc aaacgttgca atcactggcg
    18241 agcttgttat gatggttggc ggaaacaaag aaacatttga aagatgtaaa gaaatttttg
    18301 acgtaattgc aaacaagatt ttttatcttg gtgagaatgg aactgcgcac gcagtcaagc
    18361 ttgcaatgaa tctccaaatc gcaatgctgg cacttgcttt atctgaagga atcacacttg
    18421 cgagaggagc ctcaatcgac ccagaaatat ttctgaaaat cctcaactct acatatttta
    18481 agaccggtat gagtgaaagc aaggcttaca agatgataaa ggactccttt gagccaacat
    18541 ttacgctatc aaacctcaaa aaagatttgg atacaataaa ccaagcagct gaggcctttg
    18601 ggttgagctt gcccatggca aagaaagcaa acgaggtata ccgaatggcc gaggatgctg
    18661 gttttggtgg aattgactat accggcatat tggcatatct aaaagaacat gcagaaaaat
    18721 caagctgatt ggttgctttt cttttttttc aggtatgtct tatacactat tgcaactact
    18781 gcaaacaaga cggcagacac tacagcccaa ctaataaagg gaacaatctg cacttcggcc
    18841 atattatcac caacttggaa taaaataaat acatttgata tttgatatca catatgcctg
    18901 aaataatttg ccaagactgt ggaaaaaaga tgtttcatga gaatgaagaa actgtaaaga
    18961 tcgaagaagc agtacacaaa aaattctgca gaaaaaaacc gggtgaagca tacagcttta
    19021 tgcaccgtga tccccaacac acatcaacat ttgatgcgga aagagcaagc gatcaaaagg
    19081 gcaagccgat tctaaagtga tttgagcgta aacaatattc tcaaccaagc aacaattgaa
    19141 ttcaatttgg gaaattttaa aaaagcactg gcattatttg aacaagtttt gcaactagaa
    19201 ccacgacaca gtaacgcact aatcaaaaaa ggcaatatct tgggaaaatt tggcaagtat
    19261 gacgaagcca tcatatgcta cgacaagata cttgaaatgg agccccagaa cgcccttgcc
    19321 ttggtaaaca aaggtctcgc actacattat ttggaaaagt atgattccgc aatatcttgc
    19381 tacaatacaa tcctgcaaac aaatccaaac aatacaattg cgttgtacaa caaggcatct
    19441 agtcttatcc gacaagacag aatcggggaa ggattagagt tattgaaaga aaccatcaag
    19501 cttgactact catacaagta caaggcaagg tttgatatcg actttgcaca cctacagaaa
    19561 aacaatgatt tcaaacggat agttttgtga atttcgaaaa aataacttaa taatattcaa
    19621 atacagacga ctgcctgttg gccaatgaac tgaatttcga tgtcgtagta gtcggggcag
    19681 gtcccgctgg ctcatctgct gcacattctg cctcaaaaaa cggcatgaat gtggccctac
    19741 ttgaaaaaga agagtcggtg gcccaaacgg ttagaacaag tggagttact tggattgata
    19801 gcatcgagga atttggaata cccgaggatc gctacaatcc gatcaagaac tatacgtttt
    19861 gttcaccaaa caacgagatc acaattggca gtgataagcc aagagccgct gtcctggatg
    19921 tgagaaagac ataccgctgg ttagctgaac aagcccaaaa atctggtgcc gagttatttg
    19981 taaacactat ggttacagat gcggctaaaa ccactagtgg aatttcatta aagacaatat
    20041 catcaaaaga agaaaccgta ttcaacgcca aaataataat tgacgcaagt ggatttcaga
    20101 gcgttgttgc aaaatcgctt ggttttgttt ctcagtggaa aagatttggg gttggtgccg
    20161 aatacgaagc ccaagtcgaa aatgtcgatt ctagcacatg gtggctcatg gttggacagc
    20221 aatactcacc tgctggctat gcatggatat ttccactcgg aaaaaacatt gtgaggattg
    20281 gggttggcgt aggcaagccc gaatctgcag ttgatcctac cgaaaggcta aaggatcttg
    20341 tggaaaaaag agtcggcccc attgcaaagc ttggtaggat cactcccctt gaatttcatt
    20401 atggattgat tccaaatgac ggtctttcac gaaaaacagt ttatgacaat ctgattttgg
    20461 ttggcgattc tgccgggcag gcaaatccac ttgttcttga aggaatcaga tatgcaatcc
    20521 ggtttggcag gttagccggg caaattgccg caaaggcaat ctcaaatgac aacacttcag
    20581 aaaaggcact tttgccatat gaggaaacat ggaaaaaagc aatcttaaca aagataaact
    20641 ctgcatcaaa ggtgcaaacc cgctggattg gactttcgga tcaggaatgg gatgaggagc
    20701 ttaacataat aaaggagctt agcgttgatg agtttctgga ttttatcagg gcagattttg
    20761 ggcttgccag cattgtaaga cttgccacac atcatccaaa acttgcagtt agacagcttt
    20821 tcaacattgt aaagagtgct gcaaaaagac ccagtcagca tgaaaaaagc cttacaccat
    20881 aagaaacaga catcgaaatt agttacttag aatttttttc tacgctacaa ggatttgttt
    20941 ttgaaaaatt ccagttggaa gtattgtcgg ttcttcaatt tttggaatct ctggcataaa
    21001 gcacagtgca tgcacttgtt cggctgtcct agtaccacat tgtacacata tcatacttac
    21061 tgcgtctcta cattctgcac aaactgtgca ttttttgagt tcgcctccgc aatttcgaca
    21121 tgattcgtct ggcatttttc ttaattacga ctttgttgct acgaatataa gacttgagta
    21181 gaattcgttc gtatcaaagc tcagtggtct gctttggcat ttgcttttct tgattttctc
    21241 acatacataa taattccaat tattataacg ccaattatta ttgagaatac tccaacgttt
    21301 tcggtttttt gtgttttttc agtgttttgt gttacgacgg tttcagaaat ttcgccacca
    21361 tcagatgcca ccgcaagaaa gcttgtagta taaatgtcag gcctcaagac agaatcagaa
    21421 attgcataca tctttagatc gttcccgcca agccccagat tattggttag ctgatctgat
    21481 aatgtgattc ttgtcttgtc atcttgtatt agcaatatgc cactgtccac ctgatcacct
    21541 gccgcattat tgataaaata atgtatttgt gacgcatctg ttgtttgaat tggaatctcc
    21601 accgttgttc ccattacaat ttcatcaggt acatcaattt ttgttatttt tggcagatta
    21661 acatgctcaa actcactcca ataaccagct acaaatggat atgaggggtc atcaaacgcc
    21721 ctaatcgtga ttgttcttgc gtcaggagaa taaccttcaa gatagaacgg cccgttgctt
    21781 attactgcat ggtttttttc ctctatccag ttaattgcag actggtatct tgcatcatag
    21841 tattgttgag ggttttcaag tacagataat gccacagggg ttttttttga tgcaagaaaa
    21901 tcctctagat tttgcctaat tatggttgcg tcacgaggta tgattaagga aagccagttg
    21961 agactctttg cctgtgaatc agttcttgaa aatgaggctt cgccatcaag tacggcttgc
    22021 tccattgcat acattatctc ccacggcatt gccacccaca tcccgctcca acttgcaatc
    22081 tcagattcgt caaaatgcca ataattgaca taaacttcaa ctgtgtgctc gtttattgat
    22141 cgtataccaa caagtgtttg tgcaacttgg cttgcagatg gtgaaaagtc aggatcaaat
    22201 gtcttgtcgt tttcctttga ttgcgaaccc cattcttgtg taaaatatgt cgagtagaga
    22261 acatcgttta gatccatttt ttgtccatga tgccagttac ccaacatcaa atcaaaagta
    22321 atcttgctaa tggcctttgt atttggttca accattaccc atttttgcaa gtcagaatcc
    22381 caattcatga cattagtagg cacatccagt gctccactag gccctgctgt ctctaccttc
    22441 cagtcttctc tgacagcaaa gttttttcca gagtatggat ttttgaatac ccccggatca
    22501 tacagtgtat cccaaatgtt tttgctgtat gcatcgccaa gacctgcaat tggattccaa
    22561 gccccttgat agatttgttt gactccgatt ttcaactcgc catcatcagt tcttgcatta
    22621 attggggtga atctggtcgt tatacctccg ccgaaatcat ttatgacacc atttacattt
    22681 ttgtttgcaa caaaaggatc aaccttactt gcaagaaaaa ttcgtacaga gtcacctatt
    22741 ccttcttttg ttgcggcacc aatcagttct gctctttgct cagcagatgt aaaattacct
    22801 gaatagattc tctgtgtaag atcatcaatc ttttgattct catagttcca atatgttgga
    22861 ttgttaaacc caggcatgtt agaaaaccac ggcgcataca tttgtgcaag cccgagtgga
    22921 tcatacttga caaatgcaga acgcccagcg tagccttcag tgtacatgct ccactttaat
    22981 tcagctggat ttgagccgta aactactaca aaggccttgt ttaggtcacc aaagtccttt
    23041 tttacaacaa agccaatttt ttcaagttct gcagatatga cctcaccaat cgattttcgt
    23101 acaggatcat catttctaat aaaaatggta atttcaatcg gtttgtcgtc atacagccat
    23161 tttccatcaa ttttttttgc gccattttct gccaggatct ttgtcatcat gtcgtttgca
    23221 agctctggat tgtacctaaa gtgaaatttt tccaactcat ccacaatcag aagatagtct
    23281 gggtcaaacg gaccatagtt agatgtcatt acaaccccat ttccccccat tagctcatca
    23341 acaattagtt ttctatcaat cagatagttt aacgcaaatc ggatttgttg gaatgagaat
    23401 gggttaaact tttctccaac agctgggtta acaagcacac tgtatgagcc acccgttgtg
    23461 taaaagacct gcagattttg caatgattct tgttcttgta ataactcggt aggaattctg
    23521 gaatagtata tgtccagatt ccccttttta acctcctcta gtgctgtgct ttcctcaaga
    23581 tattggataa actgcacctt gtcaacaaac gttccttttt ctgcaaaact cgtacctatc
    23641 ataaaagaac acatcaaaaa aatgagaact aatttcacaa gctagataca aaccaatcta
    23701 atttaaagct cttagcacat gcaatcatgt gttgcaatta aacattgttt gttgcaacgt
    23761 ttaaaattaa taaaatgaca aaaaatgtat gaatcaaaag ttatggctta atggtattga
    23821 tggcatcata ccaggagaca tcagtgttca agattttgca attatcttaa actccgatca
    23881 agagactgct tggaaaatgt tgcaagaatt atcgcagcat gacattggta aatttgacgg
    23941 ggaaacaagc gagtttgtcg aaggagacaa actacgagcc acaattttag ccctcaaaaa
    24001 gggcgttcta gtggacgagg ctgcaaagca tcttgggtgg aaggattttg aaggtttgac
    24061 tgcacaaatt cttgagttaa aggattttac cacgttaaga aacttggttt taacaaaacc
    24121 gcgcatggaa atagatgtgg ttggaatcaa gtttggtgtt gctatattaa ttgactgcaa
    24181 gcactggaaa aaacataccc aggcatcact tgaacaggca gttagaaaac aaattgaacg
    24241 cactaagcac tatgttgcaa agacaggtgc aattgccgca ccagccatca tcacattata
    24301 ccaggacaca ctgagtttta tcaacaaggt tccgattgtg ccaatttttc agtttgcatc
    24361 ctttgttgac gaattttacg ggaatctgga cgatgttgaa attatagaaa aagactcgca
    24421 ataaacaaag caaagcttcc aaccatgaat gcctttgtta ttgctagaga tttgtccagt
    24481 gcctgtgata cttcctcata gattgcagaa cccatcacct tgatttcggc tttttgttcc
    24541 atgacttcaa acgatgcagg agtaagattt ttttctgcgt cttgtgcaat tttgaaatac
    24601 catccctcta tgtcatcgaa ttttgtgatt ttgccaaatg gatagtaacc tgttctagtt
    24661 ctaacatgtg ttgagctgta gtgtgtatcc gaggtacaca tctccaaaag actcatccca
    24721 ttttttgaaa aatgatcaac tacccgttcc cttaggccgt tttccatgtt attagagtca
    24781 gcccagccaa gaaaatattt taaattgttg atttttagac acaacacgcc gattcctccc
    24841 ggccctaaat ccattacatc taatttcatg ttatcagaat tcgcataacc aaactctagt
    24901 ggatggcttt cttttgtaat taatgtttca agtgttgctt tggcagcttt tagcatgtct
    24961 tgtgagtctg gctgtgaaat gtcctttccc attgcgttgt ggcagtcaac tacaagcact
    25021 ttttcataat tcctattgct tgcaaactgt tcaatttctt ttttgatgta gtttggcaca
    25081 tcttccatcc catgtggcga aagtgacaaa aacaagacgg ctgttttgtc aaaaagaagc
    25141 ccagttactc tcgctttgtt tatctgcaca attacaggtt ctgtacatat ggttccattt
    25201 ttgatgacgg aataatttga aaggctttgc agatagttct cgacctcatt ttgtgaggga
    25261 agatttaacg catgatcaga tacgctatgc attaccattg cacaagaatt catcgtcttg
    25321 aaaatcctgt atgttatatt actaccaccg attgggtggt atggacctgg gtgaacttcc
    25381 ggcagaacca ttctaaaatc attatctttt gagcgtaggc gaatttgcga ggtaaaaata
    25441 gtcagaggtt ttgattttga ttcaaagatg gactctactt caacaaaatt tcgttttgag
    25501 attgatgcaa gatatgcttg tagaagtttg tgcgtgcttt caactcctgg ccgtccagtc
    25561 ctatcagtaa ctgaagacca tgtggatgca atcacaataa aggtggcacc aattcccaaa
    25621 gcaagttggt ccccaagtga gctagcccaa agatctgctg ggactaaaac cagatacaac
    25681 gcaagcggtt gcaaaaagca aagagtcaat gctttcttca atgagacccc gagtgttgtt
    25741 gtcattatcc cgattctaaa gctggcaaaa atcatcattg cttctgtgac ataaaaaagc
    25801 gacatgtcct ttgatagaat cgtcgatgct gcagccccag acaatatgat tgccagccac
    25861 aaaagatttc caaaaagcga catgtgtagt gatttagaat attccttgtt tttgataaaa
    25921 cgagagtcga tgtactgtgt taccgccaag accccaattg ccgcaggtag tcgaaataga
    25981 atttcgtcgc ttttccccaa atatacaaag agtatgacaa acgaggcaat tgctgctgca
    26041 ataattgaaa ttattagtga atggtatcta gacgtgggat tcagcagagt aagagagtat
    26101 ctcctgtgaa tgtttgttac atcgtcgtgt gattctgtca tgtacgattc ttattttatc
    26161 aaaaagagat caagcatcca tgaaactatt attagtccaa taggtattga gacaactatt
    26221 cttggatcga gtctgtaacc ttttgtttca tcctcaaaga agcgcaataa tcccgcagaa
    26281 gacgcaggaa gcggcgcacc ttttttctta ctgctcattt aatgaaaaag ccgtccctta
    26341 aaataaaaac ttttgtatta tttctgcagt ttttgaatag tctcgattag cgaattaatc
    26401 gattttagaa tttgttgctg tttttcatag tcattttcag attccagttc ctttgttagc
    26461 tgctcaagct ttttgttcag agaacctaca aatgagcttg ttttctgatc aggggttgcc
    26521 tgtttttcag atcctagttt gatctcgtcg agtttttgtt tgtctggcgt ttttccgcaa
    26581 ttaatacaca aagcgttacc gtctttcatt actcgaaccc ctttacaata cggacatggt
    26641 tctgcaacaa gtgttgcacc ttttagtagc atctctactg ctttttttcg cagctcttgt
    26701 gacattagtt atttatgatc aaagcttggt aaaaacctag tttcgtcaat caccccaata
    26761 aggcttaata ggccgattga agacaagtat actagaacta atggcagtaa tttgcaatat
    26821 ttgtggactt cctgaagatt tgtgcgcctg cggtgatcta aacaaagaca gtaaaaaaat
    26881 tgttgtaaga cttgaggaaa gacgtttcaa aaagaagggc actatgatag aaggtcttga
    26941 cacaaagcta aacgatgtgc acggaacgct tagcgagctt aaaaggcgat atgcctgcgg
    27001 cggaactgca aaagaagatt acattttttt gcaaggagat catcgtgaga ccatgaaaga
    27061 aactcttgtg aaacttggat tttcagaatc aagcatcgaa atacactaat cttgcagcct
    27121 tacaagcttt ttcaaggaat cgcaattcca tattccgctc tagtctcagt aatctttggc
    27181 ttgatgattt tttcatttcc aattggcgca tacctttttt ttaattcaca tcttgggaaa
    27241 agcattgact atcactatcc gctctccgag ttggagtttg tacaacattt tggcctcaaa
    27301 tcactagcag caattaagat tggtgatctg tttgttgttg cgtgggtgtt ttttctcatc
    27361 ttgtttgcta ttgcagcttt tggcccaaag aagagttttc ttaaagtttt gtctccaatt
    27421 atggccggca cccatgagag tcaagatggt aattatttag ttcacacgat caagtggttt
    27481 tcaataatca tcattttatc agaagtaata aacaccatgc aggagtttgt cggcatcaaa
    27541 accactttgc cgccatttga gaatgatctt ttacagtttt tgtctgtgac agtcgcgcca
    27601 gtaattgagg agattgggtt tagaattatt ctaattggaa ttccgttgtt tttgttttat
    27661 tcgcataaag cttctgcaaa acactttttc aaatcattgt ggaatccttt cgcaaattta
    27721 ccaatttcca attcaaaaaa accaatcgtc atcatagtaa ttgtcggcgt attttttggc
    27781 gctgcacata tcatcactga tcagtggagc gagggcaagt ttgcacaggc tacaatgagt
    27841 ggaatcataa tcggttgggt ctactacaga tatgggtttg tggcatcagt tcttattcac
    27901 tgggcaacta actacatgat tttctcatac gggtatttgg tgtcgactgt aaatgaaacc
    27961 agctttgtgg atgcattctc acattcgctg ctccaaacta tcgagatttt gtttttagtt
    28021 actggtgcac tatcgatcgc attattgtcg ctagaatatc aaaaaaagaa gctagaggtt
    28081 taaggctatt ttgaggccct tgtagcggtt tcgtatcgta acttctgtaa cacctgccgc
    28141 ctctgcaaca tcacgctgtg ttttgttttc accgtttgtt acacatgcaa catatagggc
    28201 ggctgcagca aggcccattg ggtcctttcc tgcagatatt ttgttttctt cagccgtttt
    28261 caaaattctt actgcttcgc gttttgtttt ttctgagagt tccgccttac ttgcaattct
    28321 ggcgacacat tttattggat caactactgg catttttagg ctaagctcac gtaacaacag
    28381 ccgatagcac cgtgcaatgt cttttcgctt tatgttactt gcatttccaa tgtcttttag
    28441 cgttcttggg gtttgcgtat cacggcacgc tgcatacaat gcagatgcaa taagtgctga
    28501 aatagagcga ccacgtacaa gtcctttttc taatgccttt cgataaatat atgcggcttt
    28561 ttctattact gcatcaccca ctgcaagctt gtcctttagt ctgtcaagct ccgaaaatgc
    28621 ctgcctaaag ttcctatcca cgggttcatg aacttgactt ctcgaatccc acgttcttaa
    28681 tcgttcaatt gttgaacgca tagttgatga aagtggtttt ccagttgcat ctttgtctgc
    28741 ttggccaatt attgttgcaa gacccatgtc gtgcattgcc aatgatgtag gcgttccagt
    28801 acgcgttctg ttttcacctt cttcttttga aaatgatctc cactctggtc ctgcctctac
    28861 attttcttca tttagaacat aaccacattt accacaaaaa ttttcaccag tgttggtgtc
    28921 tgtgacaact gagccctgac cacaacgcgg acatcgatct tttttaccta cggttttaac
    28981 catttttcgc ttaccctttg tttcttgtat ttaacctttg ttgctaaaaa cctgtcgatg
    29041 gttttgacag tcctaaaaat gaataactga aattatcagg tttgattaag atggtttcca
    29101 gccttttggc atagagccac gtgaagaatc tgattttggt tcttcttttg gaagagattg
    29161 gccttgttga tcatatactg actggtgttg ttcatatcca gaatcggccg caagttctgc
    29221 ttcacctctt aatgtatggt atccgccaga ggcttcacta gtacttgagg cccgcagatg
    29281 ttttggatca attccccgtt ggatgtattc ggtttcttgt tttgcctttt tgttggacca
    29341 gtagaagaat cctccagctc cggctgccgc cattcctgcc attccatata tgataaagac
    29401 tggaaatgag ccagtcgatg tctgtgcagc agtagttggt gcagttgcag aaactcctac
    29461 tacttcatgt ccctgtaatt gatcaggtga tgcataacca gcaaggaaaa cgtttgccga
    29521 gtcgccagac tggactgttc ttacatgata agtcgcatct gttgtaaagg ttgcctcaaa
    29581 ttctttttct gaaacaatgc cttctctgaa gcttgattct cccattgtaa atgaggagac
    29641 gactactttg gcgccagaaa atccgtattt gcttgtttca gcagagacgc ccgtcgggtc
    29701 aaacaaaaag tgccagtttg ttagcggttg gtttccaatg cctgaggcat caattagact
    29761 cattgttaga atatcttccg cttctgttcc tgcaatgctg gaggcaacgt taggaaagtt
    29821 ttttgtgaga aatgaaagtg gttggtttac ttctacttgg ccatattcct cagtggaaat
    29881 agttattggg ccatcaactt tgagacctct ccaagtaaga tcaaataatg caggtgaacc
    29941 ttctgaatat tccctaatta agaatccgtc aattattggg gtaagtataa ctctataatc
    30001 aattgaaacg ctgttgtctc gtgcgaacat tgaggcgctg tagtcaattt tgacagccgt
    30061 tacgtgtgca gaactttgtt tttgtgaaag actttgattg agttcatcaa ttagttcttg
    30121 aaggtttgtt gttgaagaat ctgcagaaac gaaaatcgat atgtcatttc ctcttaatgc
    30181 gtctgcaata tcactatctg ccggatattc tatgaacaca ctcctttgga agacgaattt
    30241 tgggttttgt gcagtctcaa atgtctttgc atcccagtta agctgcgcgg cccatgctgg
    30301 acaaactgtt cctacgacta aaatgctcaa aagggtgaat gccagtagcg aagccctatt
    30361 cttcacaaaa ctcatgcttg gcgggacagt aataaatcta tcttgataat agataaccca
    30421 aattttgtaa aaatgaaatt tggatttttt tacgattttg ttgttatttt gacacaagtc
    30481 gtaattattg aaaaggtaat attgatccag actgccatac aaaaagaggt tttcagatta
    30541 tgataacaat acttgctggc ggtacaggtt ctgttaaact tgttagaggc gttatttcgc
    30601 aatcaagaga cgtcaatgtt gtttgtaatg ttggtgacaa ttattggctt tatggaatgt
    30661 ttgtttgccc tgatatagac accatcgtct atggtcttgc agacatttta gattatgaca
    30721 aagggtgggg gattagaaaa gacacattcg gattcttgcg acaaatggaa atttttggag
    30781 aagaaacatg gtttagaatc ggcgacaggg acgttgccac tcatctagtt agaacgaaca
    30841 tgttaaagaa tggaaaaaat ctcgcagaca ttaccaaatg gatgtgtgaa aagtttgccg
    30901 ttgaagcgaa ggtcatgcca gtaactgaca acacgatcga aaccagaata actacaaaca
    30961 aaggagaaat gcacctgcaa gaattctggg taaagtacaa aggaaaagac caggtggtag
    31021 gcatacagta tattggcgca gacaaggcaa gaccaaatcc agaggccatc aacgcaatac
    31081 atgattccga attagtaata attgcgcctg gaaacccgct tacaagtatt ggccctatgc
    31141 tccagatcaa gggagttaga aaagaacttt caaaaaacag aaaaaaagtt gttgcaatca
    31201 gccctcttgt gtctagcaag gcgttttcag gcccagctgc agaatacatg caggcagccg
    31261 gcattgaggt atccccctat ggtatagcac agatgtattc tgatgtttgc tcaaacattg
    31321 tgattgactc aaaagacaga ttacttacaa agaaaattca gaatttggac atgaaagtct
    31381 acgacaccaa catcaaaatg aataacaaga ttgccgaaga cgctctggcg tcatttgttc
    31441 taaaaaacgt ccgcgtatag attgcaagta gctgccataa tcccagtaaa gagattttcc
    31501 aacgcaaaga caaggcttgg ccttccacaa gaaaaaacag aattgttttg taaaataatg
    31561 ctagatgagg ttctctccac agtatcacaa tcccaaaaaa taaacaaaat cattcttgtt
    31621 tcaaatgacg agtcggcatt agagatgggg aaaaaacaca atacagtttc catctttgag
    31681 gaaaaggaaa ggggtgtaaa tgccgcagtc gctttggcag aaaaatacct actcgagaat
    31741 gggtttgaga catcggttgt atttccacaa gacattccgc ttatgcaacc agtagatatt
    31801 gacaacctgt ttgcttttca gaaaaatacc aaatcattgc tcattgtacc ttcaagaaaa
    31861 tttgacggga caaatgcgct tgtcagaact cccgttgaca taatagagac acattatgat
    31921 gaagatagct acaaaattca cttatccaca ggaaaattgc gtggaatccc cacttcgttt
    31981 gtattgattc atcgaataat gtgggatgtt gatgataggt cagatattgg atacgttatg
    32041 aacagtattg aaaagccaca aattggagat aagctcagaa aaatattgga gtaatgaatt
    32101 atgctaccct tgcttagtat tgctttattg gttgcattcc caggaggcgc aagcctaatt
    32161 cctggaggct ctttgacttt ttacgtacat gatgatgacc taaacaccaa ccatcgtgga
    32221 atcgatgaga taacaactgc aggcctttta gaatttacca ttaatggtgc gctgatttca
    32281 ggcccagcaa agattattga gaccagtggc aattcaggag tttttgttgg aaagatcagc
    32341 ataccgacat cagtaaatgg aaagcatctc aaacaaggcg acacacttgt aataaaatat
    32401 aatgacgagt cagatgattc tggaaatccc aatactgttg ccagatcaat ttcaattgca
    32461 aaagccatta ctgattttga cacgtcgcca agcaagattc gaattggaca aacattccag
    32521 gtaaagattt acgatccaga ctttaatttt gattcaagag aagcagacaa catacctctg
    32581 agactagttg agttccgagc agctaatggc attagggcaa ccttgataga cgacgccttt
    32641 aacgcaaagc caaaatcttt gagggaaact ggagataata caaatctctt tgttgttacg
    32701 atcaagatgc caaaggagat tgatggtaaa aaactcaaag ttggctctac tgcagagctt
    32761 aggtttactg atacaacgtc accgtctggc acatttgaga ttctaaagaa aactgtcaga
    32821 ataggctaat tttcaaatag ttgcgtgctg gccgtaaccc tccttctgtt ttagacgaca
    32881 tttagctttg cagcctacct gaaccgagtg caggcgcttg tggttcggtt tggccttgct
    32941 ctgcgcggga cagacttttc attcctttct gctggaaatc tcaggggttt ccatcattgt
    33001 gctccagtcc ggattttttc tgttctgttg ccagccatct ctgaccttct gtcaccagaa
    33061 cgcgcttcct gcattgaggg aggagtttcc tctgctttcg cagtaatcgt ccaccagctc
    33121 gcaattatta ttttattttg caaccccaat atttaggtga ttatgatttt gaatcgaaat
    33181 gttcctcgtt gaatcatttt ttgtgtgtag attaatgtgc aaatcaatca gccagtgttt
    33241 tttacgagcc catattcacg caaaagtggc tcaatttcct cttctgaagt catctttgaa
    33301 taatctttta acagatgctc attaagctcg taaaacgtat gaccccaatt gaacttgccc
    33361 aataattcca ttgtcatatc ctcatatccc aaaatgaaga cagctgctgc aatcgcttca
    33421 acagttgtca gtttgtttag ctttgaataa ttgactggat ttcccgcgag tagtggtgga
    33481 agttttcttg gaatgcctac aaattttttt gcaaatgcat tgtctgctcg attccatgag
    33541 caatcgattc cagtaatgga gtgtgctatg agagcatctt ttcttagcag cgtttgtttt
    33601 gcaaacggat ccaaaacaat tgtgtcagtc tgcgtccttt ggatgctttt tgcaaggcca
    33661 aacttgacta gttttgccgc agtgcatttt ttcggatcgt cttgcctaaa catcaacact
    33721 tgaacgttca tttaagatac tatgaaaatc gtgaaatatc ttcttactgg atcggtttgt
    33781 agattatctc gtagtagaac ctagaggact ggttctcttt gatgatttca acagtccaaa
    33841 tcgagtttgg aatcacgccg tcttcagatt ctggagtaat tgagaagttt tctacgaacg
    33901 gttcagggcc agagtatgtt actttgtagt caagcccagc aaacgatacc acttcatagc
    33961 cgccctcact ggtctttgta gattttgaga caagacagcc ttcgtcttga ccaataacac
    34021 atgagccgtc atcagccata accttgaggt ttactgattt ttcagagcct cttgttgtta
    34081 tgactgagcc ctgaattaac catggcacta cgtcctgatc ttgtacgttt tgctcaaaga
    34141 ttggaatgtc aatcatagta tcagtaatcc tattgaactt gtccgatatt ttgccaagtg
    34201 gagatactgt cttttgtggt tctttctttt caatcacctt gaatgtcttg ctaaatgtac
    34261 caaactctgc attcaccttt atcacatagg tgccaaagtc taaagaactt ggaagcttgt
    34321 aatcatactt gtagagtccg ttgccatagt atctagtcag gtctattttt ttgcctccaa
    34381 gaccacaatt agatgtacca cagttgatct tgctttcttc tggaattacc actagatcaa
    34441 gtttaccaag agatagaatc ttgtttgttg ctccatagat ctgaactgta tcaccaggag
    34501 aatattcttc tttgtctgta ttcaaagtga tggatatttt tccatcccca ccaaacggaa
    34561 tgtcgtttct gacttcaaat ctagtatctg ttattagttt ttgatacgtt gcagtaacct
    34621 tgtatgttcc atctttgaag attgttaatg gtaaatcata tgttgcttta aaattacctg
    34681 cgttatcaaa cgacaccatt ccgtcataga tctgtttgtt ggccaaagtc ttgacttgaa
    34741 ttcccaccct gtcaggaatt actccgctgc ccaaacctgt tccctgttgc cttttgattg
    34801 cattgcccaa aacagtgagt ttttcccctg ccttgtagac atccttgtcc gtagcaacag
    34861 caagcggttt tatttcgagt gtgtcagttt ctgggttctt tgatactttg aaaaagaggc
    34921 ctattgtttc agaggttgct gaaattgtta atttgtagtt accaaatacg cttggtcttg
    34981 cctccctagg atcacgtatg tctgccaggt catagtcttt tgatggaaca gtccagctcc
    35041 atgaaaactt ggaattgtca actttggcgc ctgagttaac agtcttacca gccggtgttg
    35101 tcaaaattat ttttactgca gattgtccag aaagcaacgt tccttggagg ctgactgttt
    35161 cgccaagacc atagatgctt ttatcgagtt tggcattgat gtttttgtca gtcgtatcca
    35221 aagttccact tactgaaaat attgtagatg tcacactctt accatacttg gcttcaataa
    35281 tgtaagcccc aggcgtaaat gtcgacagat ttagtggcat atctatctta tagtacccag
    35341 cagtatccgg aattgcagta aagctgtacg caacaattag actgtcatct cttattttga
    35401 gcttcttatc ttttgcaaca atagttaatg gttttccact ttcatcttgg attgacaccg
    35461 aaacaggagt tgtcacaaat gtactccttg cctctggaat cacatgtcca attattgtta
    35521 gcttttctcc atattcataa
//
LOCUS       DASWKH010000107         5361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3242814, whole genome shotgun sequence.
ACCESSION   DASWKH010000107 DASWKH010000000
VERSION     DASWKH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242814"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VGA92_08150"
     CDS             <1..1523
                     /locus_tag="VGA92_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9846414.1"
                     /translation="NWDAYKTEANQIQVETIYNRDVVNALNYVLEKNTEMILTTDSLV
                     RDLELLDRNYNRHKEIAHQLQETAKTVGQNALLVSIGQENGTKENLHNARISFDVGIR
                     KLLQVPLDDLEITGLQIEEGILEPIPRENSQSLDQLDLLWEAVGLRVKTLETKSLYSD
                     EFRQAYDSLNEQRQSLLDSIDELLAMWNENRLERRSQGELITQSIIGIDLAVFAMVLI
                     TIRKSLTPLENITRAISRVKEGIYGEKIEYTSSDEIGELASSFNTMSETIRIKEEEAK
                     RTDIAKDEFLAMITHELKTPLVPIQGYADILLSGYLGNLTEKQRERIAIMKSSATSLL
                     QLISDLLDAQKLELGQLRIKKNPADIHATVTKSIQTLNPEIEGAKIKVVNEVNQNLVV
                     PHDTERIAQVLTNLIKNSLKAIKPETGLIKIYSSEDQNEIRIIIHDNGLGIPYERQGK
                     LFTKFYQADASLTREKGGSGLGLSICKGIVEAHGGKISLESKPDTGTTITFSLPKH"
     gene            1618..1764
                     /locus_tag="VGA92_08155"
     CDS             1618..1764
                     /locus_tag="VGA92_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846415.1"
                     /translation="MEETDPPKNTEFYAVCNEYFAALRKQRRNDDGFEDEFFYTMPVI
                     SGSN"
     gene            1799..3202
                     /locus_tag="VGA92_08160"
     CDS             1799..3202
                     /locus_tag="VGA92_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9846416.1"
                     /translation="MLEQAEKAVELAQKHGCTYCDARIETSEKHGLVIEDGQIEHILT
                     KNEQGIGIRVLCDGAWGFYSTSDFHKLEYGIIAAIKSAKHYAVKKKDGVKLADIVTSQ
                     KKIKYKAKKQADIDSLIKIAFDCDKIIKDCKLISKSTVSASNSTTSKYLASSEGTKIQ
                     QDFSDVIIDLVATSRQSGITQSINTTEGGRGGLEKITEDVDIYSISGEIAKNAAMLVN
                     AKTAKEEKATIVMNPDFVALLTHEILGHPSEADRVLGKEMAWAGGAWWAGMLGKQIGS
                     ENLNVIDDPTIEGSLGWSEFDDEGTKSQRNQLVKNGRLVDHMHSRETANIFGVRPNAS
                     MRATSYSFMPLIRMSCTCIEKGDWNPDEMIKDVKHGYLVSNMKVPSIDMKRYNWSISC
                     QYANRIENGEIKEPLRDVIVVGAAPEFFDSIDACGKDFTVRPITNCGKGDPMQQMKMG
                     NGGPSIRGTATIKSVGG"
     gene            3204..3761
                     /locus_tag="VGA92_08165"
     CDS             3204..3761
                     /locus_tag="VGA92_08165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9846417.1"
                     /translation="MTELEKIRSQVISCTKCDLCKGRTNAVPGKGNPNAEIIFVGEAP
                     GRTEDLKGEPFVGVAGKKLTEMLEKNGIPRESVYITNVVKCRPPNNRVPTESERLTCR
                     EYLEKEIQLINPKIICIMGNTACTSILGQGEITKNRGKIIKKDGKTFFLTFHPAATIY
                     NQELIPLMEKDIAALVEALQKTNGE"
     gene            3751..4320
                     /locus_tag="VGA92_08170"
     CDS             3751..4320
                     /locus_tag="VGA92_08170"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9846418.1"
                     /translation="MVNNSRLPREFYARNTVDVAKDLLGKILVRRIGKDTISGIITET
                     EAYRQSDDPASHAYRAITRRNEAMFGEVGHAYVYFTYGMHHCVNVVAKSKNHKAGAVL
                     IRSLTPKDGIDIMMKNRKIDNVANLTNGPAKLTMALDITKKQYGEDLVSSLYIYITNG
                     TKIDQKIIAGPRIGIKEGIDKQWNFKIKL"
     gene            complement(4317..4958)
                     /locus_tag="VGA92_08175"
     CDS             complement(4317..4958)
                     /locus_tag="VGA92_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX9846419.1"
                     /translation="MDISEIFPFFNEIGYLGLALVSFFGSLIPFVPLPSFILLATMSV
                     GDKFNIHYLALIVAITSTVAKQIIFLISYGGGRIITEKTRKRMRPFERLVKKYGSAAA
                     FVAAATPIPDDLIYVPLGLAKYNPLRFFISTLLGKLVLSYVIVLLSHFFGMAYVEPFL
                     ENVDDPTIVYVGFVFFVAMTAVSVFLLLRLNWEKILGRFAPWTVQKDDDESKD"
     gene            5070..>5361
                     /locus_tag="VGA92_08180"
     CDS             5070..>5361
                     /locus_tag="VGA92_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846420.1"
                     /translation="MIKKQHREICSRISAISPYIRFVGIIGRNGKLLAYQRRMSLRPL
                     LNAKDTQSQFSHISQKTDVEGTFDRRLGKVLFVWEERKKVQIISFAIKRNRVW"
BASE COUNT         1944 a         1004 c         1152 g         1261 t
ORIGIN      
        1 tgaattggga tgcttacaaa acggaagcaa accagattca agttgaaacg atatacaata
       61 gagacgttgt aaatgcacta aactatgtct tggagaaaaa taccgaaatg atcctaacta
      121 cagattcgct agtcagagat ttggaactat tagatagaaa ctacaacaga cacaaagaga
      181 ttgcgcacca gctgcaagag actgcaaaaa cagttgggca gaatgcactg cttgtttcaa
      241 ttggacagga aaatggtacc aaagaaaacc tgcataacgc aagaatatca tttgatgttg
      301 gaattagaaa actccttcaa gtgccacttg atgatctaga gataacaggc ctacagatag
      361 aagaaggaat acttgagcca atcccaaggg agaactcgca gtcactggat cagcttgatt
      421 tgttgtggga agcagtagga ttaagagtca aaacacttga aacaaagtca ctatactctg
      481 atgaattccg ccaagcatat gatagcctaa atgaacaaag gcaatcacta ctagactcaa
      541 ttgatgaact gttggcgatg tggaacgaaa atagacttga gaggagaagc cagggtgagc
      601 taatcacgca gtcaattatt ggcatagacc ttgcagtttt cgcaatggtt cttatcacta
      661 tcagaaaatc attgacacca cttgagaata tcaccagggc aatttcacgc gtcaaagagg
      721 gcatttatgg agaaaaaatc gaatacacat caagtgatga aataggtgag cttgcttcca
      781 gttttaatac aatgtcagag acgatcagaa ttaaagaaga agaggcaaaa aggacagaca
      841 ttgcaaagga cgaattcctt gcaatgatca cacacgagct aaaaacccca cttgtaccca
      901 ttcagggata tgccgacata ctacttagcg gctaccttgg aaatcttacg gaaaaacaac
      961 gtgaaaggat cgccataatg aaatcaagtg cgacatcatt attacaactc atttcagatc
     1021 ttttggatgc acaaaaactt gaacttggcc agctaagaat aaaaaagaac ccagctgaca
     1081 tacacgctac agttacaaaa tccattcaaa cattaaatcc agaaattgag ggggccaaaa
     1141 ttaaagtggt aaacgaggtg aaccaaaatc ttgttgttcc ccacgacaca gaaagaatag
     1201 cgcaggtttt gactaacttg ataaaaaaca gtctaaaggc aatcaaacca gaaaccgggt
     1261 taatcaaaat ttactcttct gaagatcaaa atgaaataag aataatcatt catgataacg
     1321 gtttaggtat tccttatgag agacagggca agttgtttac caagttttac caggctgatg
     1381 cgtcattaac acgagaaaaa ggcggtagcg gtttgggatt atcaatttgt aaaggaattg
     1441 tagaagcgca tggcggaaaa attagcctag agagcaagcc agatactggc actactatca
     1501 ccttttcgct gccaaaacac taaatcctag aaaataactc caactcaaag aaatcacaca
     1561 tactgtacaa atcgggctca cactgtatta gataacttga ctttaggcgt atcactcatg
     1621 gaagaaacag acccgccaaa aaacacggaa ttttatgccg tctgtaacga atattttgcc
     1681 gcattacgca aacaaagaag aaatgacgat ggttttgaag acgagttctt ttatacgatg
     1741 cctgtaattt cgggtagcaa ttaacaaaaa tagagcactc ctagccatta atgacatcat
     1801 gttagagcag gctgaaaagg cagtcgaact tgctcaaaaa cacggctgca cctattgcga
     1861 tgcaagaatt gagacgtccg aaaaacacgg attggtaata gaagatgggc aaattgaaca
     1921 catactcaca aaaaacgagc agggcatagg aatcagagtt ttgtgcgacg gtgcgtgggg
     1981 attctattct acctcagatt ttcacaaact agagtatgga ataattgctg caataaaatc
     2041 tgcaaaacat tatgcggtaa aaaagaaaga cggggtgaag ctggccgata ttgtaactag
     2101 ccaaaaaaag atcaaataca aggcaaaaaa gcaagctgac atagactctc ttatcaaaat
     2161 tgcatttgat tgtgacaaaa ttatcaagga ttgcaagtta atttctaaaa gtacggttag
     2221 cgcctcaaac agcactacct caaagtattt agctagcagt gaggggacaa aaattcagca
     2281 ggatttttca gatgtaataa ttgaccttgt tgcaacttca cgacagtcag gaataactca
     2341 atcaataaac actactgagg gaggcagagg ggggctggaa aaaataactg aagacgtaga
     2401 catttattct atttcaggag aaattgcaaa aaatgcggca atgcttgtta atgcaaaaac
     2461 agcaaaagaa gaaaaagcca caatagtgat gaatccagat tttgttgcgc ttttaacaca
     2521 tgagatttta gggcacccct cagaagcaga cagagtttta ggaaaggaga tggcatgggc
     2581 aggaggtgca tggtgggcag gaatgcttgg aaagcaaata ggctcagaaa acctcaatgt
     2641 tattgatgat ccaacaatag agggaagtct tggatggtct gagttcgacg atgagggcac
     2701 aaaatcgcaa agaaatcaac ttgtaaaaaa tgggaggctc gttgatcaca tgcatagccg
     2761 tgagactgca aatatctttg gcgtaagacc aaatgcgagt atgcgcgcca catcatactc
     2821 atttatgcca ctaatacgaa tgtcgtgcac ctgcatagag aaaggcgact ggaatcccga
     2881 cgagatgata aaagatgtca aacacggcta tcttgtttca aacatgaagg tgccttcaat
     2941 tgatatgaaa cgctataact ggagcatatc atgccaatac gcaaacagaa ttgaaaatgg
     3001 agagatcaaa gaaccgctaa gagatgtaat tgttgttgga gctgcacctg aattttttga
     3061 ctcaattgac gcgtgtggta aagattttac ggtaagacca atcactaact gcggaaaagg
     3121 agaccctatg caacaaatga agatgggaaa tggtggacct tccatccgtg gtactgcaac
     3181 aataaagagt gtcggcggat aatatgactg agctagaaaa aattcgaagc caggtaatat
     3241 cttgtacaaa atgcgacttg tgtaaaggca gaaccaatgc cgttccagga aaaggaaatc
     3301 caaacgctga gatcatcttt gttggtgaag cgcctggtag aaccgaggat ctaaaagggg
     3361 agccatttgt cggcgtggcc ggaaaaaaac taacagaaat gcttgaaaaa aatggcattc
     3421 caagggagtc ggtatacata accaatgttg taaagtgtag gcccccaaac aatcgggtgc
     3481 caactgaatc cgaacgactc acatgtagag aatatttgga aaaagaaata cagctgataa
     3541 atccgaaaat tatttgcatt atgggaaata cggcgtgcac ctcaatctta ggtcaaggag
     3601 aaattacaaa aaatcgtgga aaaatcataa aaaaagatgg aaaaacattt tttcttacgt
     3661 ttcatccagc ggcaacaatt tacaatcaag aactaatacc actcatggaa aaagacattg
     3721 cggcactagt agaagcactg caaaaaacaa atggtgaata actctaggct accaagggag
     3781 ttttatgcgc gcaatacagt cgacgttgca aaagacttgc ttggcaagat ccttgttcga
     3841 agaattggca aagacaccat ctcaggtata attacagaga cggaagcata tagacagtca
     3901 gacgatcctg caagtcatgc gtatcgcgcc atcacaagac gaaatgaagc catgttcgga
     3961 gaggtaggcc atgcgtatgt gtatttcaca tacggaatgc atcattgtgt aaatgttgtt
     4021 gcaaaaagta agaaccataa agccggtgcc gttctaattc gttcactaac accaaaagat
     4081 ggaattgaca tcatgatgaa aaacagaaag atagacaacg ttgcaaactt gacaaatggt
     4141 cctgccaagc tgacaatggc actagacatt acgaaaaagc agtacggcga ggatcttgtc
     4201 agttcgttgt acatttacat aacaaatggc actaaaattg atcaaaaaat aattgctggc
     4261 ccgagaatcg gcataaaaga aggaatcgat aagcagtgga attttaaaat aaaactctag
     4321 tctttgcttt catcgtcgtc tttttgtacg gtccatggcg caaatctgcc aagaatcttt
     4381 tcccagttta atcgcaaaag caagaatact gatactgctg tcattgcgac aaagaataca
     4441 aatcccacat acacgattgt tggatcgtca acattttcca aaaacggttc aacatatgcc
     4501 atgccaaaga aatgcgacaa aagcacaatt acgtagctta gaacaagttt cccaagcaaa
     4561 gttgagataa aaaatctcag aggattgtat tttgccagcc caagcggcac ataaattaga
     4621 tcgtccggga ttggggttgc ggcagcaaca aaggcagctg ctgaaccata tttttttact
     4681 agtctttcaa atggcctcat gcgttttctt gtcttttcag tgattattct tccgccaccg
     4741 tagcttatca gaaatattat ctgttttgca acagtagatg ttattgcgac aatcaaagca
     4801 aggtaatgga tgttgaattt gtcccccaca gacattgtgg caagcaggat aaatgatggc
     4861 agtggcacaa acggaataag cgagccaaaa aaactcacaa gtgcaagccc aagataacct
     4921 atttcattaa aaaaaggaaa gatctcagaa atatccacaa atgagaaccc ccaccgtaga
     4981 ttataaaaat gaggtgcgca agtagttgag tttggtcaat acccagttta ccatgatatt
     5041 aaaataacaa gatttgcaag aaacttcaaa tgataaaaaa acaacatagg gaaatttgct
     5101 caaggatttc tgcaattagc ccatacatcc ggtttgttgg aatcattggc agaaatggta
     5161 agctcctcgc ataccaaaga aggatgagtc tcagaccgct tttgaatgcc aaggatacac
     5221 aaagccagtt ttcccacatt tcgcaaaaaa cagacgtgga gggcacattt gatagaaggc
     5281 ttggaaaggt gttgtttgtc tgggaagaga gaaaaaaagt tcagataatc tcgtttgcaa
     5341 taaaaagaaa ccgggtttgg g
//
LOCUS       DASWKH010000108         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1778914, whole genome shotgun sequence.
ACCESSION   DASWKH010000108 DASWKH010000000
VERSION     DASWKH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778914"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1594)
                     /locus_tag="VGA92_08185"
     CDS             complement(305..1594)
                     /locus_tag="VGA92_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9846421.1"
                     /translation="MKTERLESAERLDIHNIDGDLEACLKTLEKESSKENWDLIKKYN
                     TLLTATAVSKATRRKELRTILSLNRLNNNKAWESMTQQDMITLVSDIMNKYSQDGQET
                     WSTYNHKRVLKIFFRWIRLGDRDFREVDDPPETKKIKIKNVKETLTREQLVADKDRRN
                     MIASCDDLQSKALIDTTDEGACRMAEMLTLKIKHISFDKNGAIIRVDGKTGARPIRLV
                     RSSKNLLNWVNSHPFRTNPESWLYIGIRKGVMGKRMSYSTARNILLTAAKRAGVDKRV
                     FFTLFRHSGITNVANFLSDAQMRQRHGWTKNSIMPGRYTHLVGADVESAVLKHHGLKK
                     ESQVVEIQKCKFCDAINSIDVLYCENCSKPLDIKTAIEADKKRDEDMKKQIDEAVTEK
                     LHTIIDKEMLKIKTELLEKKLKNIEDQKADRNETSKS"
     gene            complement(1679..>1887)
                     /locus_tag="VGA92_08190"
     CDS             complement(1679..>1887)
                     /locus_tag="VGA92_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEX9846422.1"
                     /translation="GFPQSFLPLDLDYVGIVHSHPSGSANPSITDLHNFFGLVSLIVQ
                     SPYEEADIFAWDSNGNQLSMTVID"
BASE COUNT          459 a          371 c          354 g          703 t
ORIGIN      
        1 agatttttgg agtctgcttc tggcttctca tcccactatc atttaacgat cgttaaatga
       61 attaattaca aatttggaat tgtataaaac aatatcggct tcgatccacg tcgtgatttt
      121 atgtttttga aaaattcctc gtcgtgattt tgtaaaatgt ttttgatcgg agggcgcccc
      181 cgccacgtaa cgaggttcaa aaagattgct ctagctaaat ttggatataa gattcacaca
      241 atatttgcat gaacttagtc aaaatgcctt acgtgatttt gttttttggt aataatatga
      301 taacctagga ttttgaggtt tcgttgcgat cagctttttg gtcctcaata ttctttagtt
      361 tcttttccag aagctcagtt ttgattttta gcatttcctt gtcaattatt gtatgtagtt
      421 tttctgtgac tgcttcatct atctgttttt tcatatcttc gtctcgcttc ttgtctgcct
      481 cgattgctgt cttgatatca agaggtttac tgcagttctc acagtaaaga acatcaattg
      541 agtttatggc atcacagaac ttgcattttt gaatttctac gacctgactt tcttttttca
      601 ggccatgatg ttttagaacc gcactttcaa catctgcgcc cacaagatgt gtatatcttc
      661 caggcataat cgagttttta gtccagccgt ggcgctgtct catctgtgca tctgatagaa
      721 agttagcaac atttgtaatt cctgaatgcc taaaaagagt gaagaataca cgtttgtcca
      781 cgcctgctct ttttgctgct gtaagcagga tatttcgggc tgttgaatat gacattcttt
      841 tacccataac gccctttctt atgcctatat acagccatga ttcgggattg gtcctaaagg
      901 gatgtgagtt tacccagttt agcagattct ttgaagacct tactagtcgg attggccttg
      961 ctccagtttt gccgtctacc ctgatgattg cgccgttctt atcaaaagat atgtgtttga
     1021 ttttcaaagt cagcatttct gccatcctac aggcaccttc atctgttgta tctattagtg
     1081 cctttgattg cagatcatcg caagatgcaa tcatgtttct tctatccttg tctgcgacta
     1141 gttgctctct tgtcagcgtt tcctttacat tcttgatttt gatcttttta gtttctggag
     1201 ggtcatctac ctctctaaaa tctctgtcac caagccttat ccatctaaaa aatatcttca
     1261 gtactctttt gtggttatag gtagaccagg tttcttggcc atcttgtgag tacttgttca
     1321 taatatctga taccagtgtg atcatatcct gctgtgtcat ggattcccaa gctttgttat
     1381 tattgagcct gttcaagctg agaatggttc gaagttcttt tctcctagta gcttttgaaa
     1441 cagcggtggc tgttagaagt gtgttatatt ttttgattag atcccagttt tcttttgatg
     1501 attctttttc aagtgtcttc aggcaggctt ccagatcgcc atcgatgttg tggatatcaa
     1561 gtcgttctgc tgattctagt ctctcagtct tcactaggca ttagatactc taaagttttt
     1621 tatagattct gtaaaaacct tataaagttt tgtcacgttt tatctgattt tttgttgcct
     1681 aatcaatcac agtcatgcta agttggttac cattactatc ccatgcaaaa atatcagctt
     1741 cttcgtatgg ggattgtact atcagagaaa ccaatccaaa aaaattatgg agatcagtta
     1801 ttgatggatt tgcagaaccg cttggatgag aatgaactat gccgacgtaa tctaggtcaa
     1861 gcggcaaaaa tgactgtgga aatcctg
//
LOCUS       DASWKH010000109        15356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_284474, whole genome shotgun sequence.
ACCESSION   DASWKH010000109 DASWKH010000000
VERSION     DASWKH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284474"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VGA92_08195"
     CDS             complement(<1..1503)
                     /locus_tag="VGA92_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX9846423.1"
                     /translation="MRPWLLTGVFASLLFFGVFTSSYAQELSDHVVINEVDTNPPGDD
                     SKAISEWVELYNPTDETVDIGGWKVASTTILKKTLTIPAGTQIKAGQFLVYSYASLWF
                     TDVSEKVRLQDNFGNVIDETPVITDQKNDFSSWQRNFDGVDSDASNDWVFRKSSVSSS
                     NGKLDTVSAGPGDLTISVSTDKQSYDFGETATISGKVSKRIYQEKPYFSQQQIKIHID
                     GPGSYDKRITLYPDLNLEFKTSLKLDKVLGVGPGTYDVFVVYDVVQDKTVFSVGETAV
                     TVITEEESELTISSDNVAYIPGQRVEISASTTEIIPLTGLEYGVFDPNGNRVFSGNLY
                     PTTKGEFSTIVYMTTVNPVFGTFDIVANYGKQHAETTFELVKDVKDTEKIVLTTDKEA
                     YGLGETVVISGRSNKFVAALDLEVLQSGATAIGKDTQNAFRIKNHVNLAGDSSFEYKL
                     QIPNDNLRYGDYRVTVSKEFGQSIVYFKVVENPEEYSAGEKGAYVSTDKAN"
     gene            complement(1631..1888)
                     /locus_tag="VGA92_08200"
     CDS             complement(1631..1888)
                     /locus_tag="VGA92_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846424.1"
                     /translation="MSYQKKEILLVAGVLFFMIGLLGAGLPLIYSVIIAVGIYVGIKV
                     FVGRRQRLIEKEIPEGVCVECGAKIISGNCPNCDAGGQKEK"
     gene            complement(1939..2012)
                     /locus_tag="VGA92_08205"
     tRNA            complement(1939..2012)
                     /locus_tag="VGA92_08205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1976..1978),aa:Pro,seq:tgg)
     gene            2087..2977
                     /gene="map"
                     /locus_tag="VGA92_08210"
     CDS             2087..2977
                     /gene="map"
                     /locus_tag="VGA92_08210"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HEX9846425.1"
                     /translation="MQIDDYVKAGKIASEVRELARAKDWTGKTLYEICETVESEIKSR
                     GGKCAFPVNASLNEFAAHYTAEPDDPKVLSDTDLIKIDLGVQINGFIADTAVTVSYDP
                     EFEVLVDAAEEALKNAMAMVKEGVKASDIGRTIEKTVKQYGFLPIANLSGHSLEQYTI
                     HAGKSIPNIWSIGSFSLSSKEAYACEPFVTTSKGLGFVREGKIRNIFGLISRKKIKND
                     AANKLLDYIWENFNTLPFALRWITKEWSQKEAKPLLDELIKNKVVRAYPVLIEANEQR
                     VAQAEHTFIPTETGAIVTTL"
     gene            complement(2967..3149)
                     /locus_tag="VGA92_08215"
     CDS             complement(2967..3149)
                     /locus_tag="VGA92_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846426.1"
                     /translation="MIFSKKEKQIIAYTAEKCPSCKNELKRKFKEGDFLFAPSTECVS
                     CKVPMVIEKIFGEPIG"
     gene            complement(3146..4252)
                     /locus_tag="VGA92_08220"
     CDS             complement(3146..4252)
                     /locus_tag="VGA92_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1512 domain-containing protein"
                     /protein_id="HEX9846427.1"
                     /translation="MLSPDTLFEGETGNPLMWLVWIIPIVIFMFYGQRIQLKITSGEI
                     NKNIEKLKKYKEDSKKGLLDYLKKNLNTTDEAPKKVEQFLEYFTIMPVDVDPNGIVPK
                     IKHLVRSREDYTRAQVKSLSPSMNALELSKIQNILEVVTSLHMLHKIVRHLYLTAKKQ
                     NNFPLILPLQMMMPFIMEQADALKDAVPALKQGQPMGDGIGPMVVGKLMLGTQKKTIT
                     FETVYGEMDFEQRKLFLLKAEGPAATVGRPGDAVEILVKEKKPNIIVMVDAALKLEGE
                     DSGAIAQGFGAAIGGIGTDRFQIEEVATKNNIPVYAIVIKQSIKEAITLMTKEIADKA
                     KEVESQIYQTIRENTAPGQNILVVGVGNTLGVSQ"
     gene            4411..4971
                     /locus_tag="VGA92_08225"
     CDS             4411..4971
                     /locus_tag="VGA92_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HEX9846428.1"
                     /translation="MPQTKPIVSIENVVASASVDQKMDLNEITRNFPDVEYHPDQFPG
                     LVFRLKSPKTATLIFTSGKMVCTGAKSEEMARSAVKSVVQKLRKGGIKVKKDAVVDIQ
                     NIVASINLGGKIHLEQAARTLPRSMYEPEQFPGLIHRMLDPKTVILLFSSGKLVCTGA
                     KKEPDVYRSVNNLHSLLEEKGLMIYD"
     gene            5039..5734
                     /locus_tag="VGA92_08230"
     CDS             5039..5734
                     /locus_tag="VGA92_08230"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9846429.1"
                     /translation="MKIDLFGDFKLKDIVLVASLPDMGRVGGLVSAHIAKKTLAKDAA
                     KVVLSDKPWVNQVDGLVEVPFDEYKILVDEKNSLVIFTGENQPQEPNAVFELVDFVID
                     TVQKFGNIRLIISTGGYLPLQKIDGDQVYGVATDQKLLESLKPHDIKTLSNDVKSITW
                     FNGLVLGAAKNRNIDGIGLFGEIIDPEAPQHRAAKNIINKIEKILKIQINTDELQIAE
                     KSPELKKDGPGIG"
     gene            complement(5735..6058)
                     /locus_tag="VGA92_08235"
     CDS             complement(5735..6058)
                     /locus_tag="VGA92_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX9846430.1"
                     /translation="MTITYDDFAKLEIRVAKIISTEKIPGKTKIIKGTIDLGSETRNL
                     IIGGAQFFEPEELVGKTVIAVTNLEPKTVGGVESNAMLLAADLNDKPYWLTVDESIPL
                     GTKIK"
     gene            6175..6864
                     /locus_tag="VGA92_08240"
     CDS             6175..6864
                     /locus_tag="VGA92_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846431.1"
                     /translation="MGQFFTDDIDALVKEGHGDHERLVRIKSDFEAKKLVTLEDRRYV
                     EGLVSRYLQPSKPESERIVKIPEKRIVPPPSPPSQNISVEMKYQKQKSQEPIPKIGGK
                     RNIRNVVVSVAVVVAALLIISVVAINRDNLGIDQPAVTENIELDQTSYARGDIISISG
                     KATTSTTVQLVISNPSNQQIWSETVSTKANGEFSTLTIAGGNGWEQSGSYTLSSTHGG
                     TTEQVTFEFTN"
     gene            6905..7321
                     /locus_tag="VGA92_08245"
     CDS             6905..7321
                     /locus_tag="VGA92_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9846432.1"
                     /translation="MATIEVSTMINAPVDKVWEIVSDIDNEPKFWKGTKQIRNISKND
                     NVVTREVTIAFKDSKCMQTVTLYPKEKIVGQFTEGILNGTKTIKLTPQENGTNLEVVW
                     DIKLSGMMGMFTGMIKKHIKNGTEQALESIKQEIEK"
     gene            7323..8525
                     /locus_tag="VGA92_08250"
     CDS             7323..8525
                     /locus_tag="VGA92_08250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9846433.1"
                     /translation="MDFVDVINYAFFAILIGVAGAWALLIKSMMESFKKTPFLDRFKA
                     KEHTHPKVSVILPARNEEGFIEKCLDSLLNQDYDNYEIIAIDDSSEDATGRIIAEFAR
                     KNPKVIHVSARPKPQGWMGKNWACMEGYKKATGDLLLFTDSDTTHSKNVISLAVSHLL
                     SLELDALTAIPKMICLDTWTKITLPVLSTFLHTRFSALRVNDSSKKTGYFFGSFYLIK
                     RSVYDAVGTHEGIKQEIIEDGALGKKVKESGYRMRMVRGDHLIDAVWARDWSTLWNGL
                     KRLMIPLYLQNSKIAVGIFFAVLFLLFMPYVLLAYSAAFFYHSQSFSALFCAALASAG
                     LLYAAGLLDARALGIKAPHGICGPIGSFVVVAGFLCGILQAKSNMAVSWRGRAYSMKD
                     HAQNSISV"
     gene            8565..9746
                     /locus_tag="VGA92_08255"
     CDS             8565..9746
                     /locus_tag="VGA92_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9846434.1"
                     /translation="MSKSTVIVSSLLATVIVLVAYSIFLNPPDNIPIVDEISKSTKNT
                     VIAQDKSILASQDLPLTEIFEKTESGVVRINVKRPDTDSRGAGGIGSGFVYDDSGHII
                     TNDHVVTNSEKIVVTFLDGRSFNAEIVGKDVFTDLAVIKVNTDADALHPLPLGDSSKL
                     RVGEQIAAIGNPFGLSGSMTSGIVSQLGRLLPSQDRSFQIPDIIQTDAAINPGNSGGP
                     LLNMRGEVIGINTAIQSESGEFAGVGFAIPSRTVQKIIPTLIESEVYHHPWVGISGRD
                     IDPDLAKVLGLQDARGFLIINVLNDSPAEKTGLRGSTEIKEIDDTSYQVGGDVILSVD
                     GKIVRKIDDILIHLQREKSVGDQMVLQILRDGKVTDVIITLEERPNSNITNNSTNSTQ
                     K"
     gene            9774..10943
                     /gene="cofG"
                     /locus_tag="VGA92_08260"
     CDS             9774..10943
                     /gene="cofG"
                     /locus_tag="VGA92_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HEX9846435.1"
                     /translation="MSDLVLGCEPLNRVLDGKEITASDAYEIFRLSSNNPLEVYKTAA
                     ILRNKHKKRVVTFSKKVFFNLVNLCSDVCGYCTYKSEPGQPKLSLMSKKDVQNLAKLA
                     KKHRCVEALFVTGERPEQKYEEAQTWLKENGFSSTPEYLAHASEIALSEGLFPHTNAG
                     NLTKNEINDLKKTNVSMGLMLESSSERLTEKNMPHHLAPSKNPKTRLRVLESAGELKM
                     PMTTGLLVGIGETAHEIIDSIFEIKKLHQRYGHIQEVILQNFQPKPDTLMRGIASAEE
                     SYFKTVVALTRVIMPQMNIQIPPNLSPNSYQSFIETGINDWGGISPLTPDYVNPEFGW
                     PEIEFVQKNTNNAGFELKARFPVYPEFMGLVSTELCDKMAQIMDEEGLVSEEYWK"
     gene            10940..12196
                     /gene="cofH"
                     /locus_tag="VGA92_08265"
     CDS             10940..12196
                     /gene="cofH"
                     /locus_tag="VGA92_08265"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX9846436.1"
                     /translation="MTSVIESLLKNSDPLIAETLDRALSEKEISVKEATRLYDASGPD
                     FHLIGMVADELRKRRVGDIVTFVVNRNINFTNVCIKQCGFCAFSRDFREEEGYFLPTE
                     EIVRRAKEAHGFGATEVCIQAGLPPDMDGGLYENICKAIKAEIPDIHIHGFSPEEVLY
                     GARQSGFSIKEYLQRLKAAGVNTLPGTAAEILDQEIRDKISPGRISVNDWVDIIKTAH
                     KLGIRSTSTIMFGHIETPEDRVKHIAKIREIQKETGGFTEFVPLNFIHTEAPMYKHGL
                     HDGIRSGAGANDVMLTHAIARIMLNNYIDNIQMSWVKEGPKMSQLLLKWGANDFGGTL
                     INESISTSAGAQHGQILRPKEIRHLIREVGRIPAQRSTTYKILKVYDSDSDYEDLDKV
                     EDISRFGSYFELVKIDKYRYKNPRAN"
     gene            12199..13554
                     /locus_tag="VGA92_08270"
     CDS             12199..13554
                     /locus_tag="VGA92_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9846437.1"
                     /translation="MSVCDDVISCAKKLGIDECESVVCARKIITVRITDSEIAEIKEN
                     YEKSLGIRLIHGKRISSCQSTILEPVKLVDAAFRSSQNLTKRDFWKSLPAGTKTDVNI
                     QKTNDQKIWQLESSGAADIAQQMINSASHQKITRISGSLNIVCEEFELQNTSQLNKHD
                     KATYVSGIINADSEHGIPVSGIGQASSRTLADFDAERVGTEAAQMCVNSINPQSCEAG
                     TSTIIFEPLAVGELLSFVFTPNFSLKTYSEKRSCFSEKIGAQISVADFSLLDDPHATN
                     RLGSKSFDDEGTPTKVTQYVHNGIFENTYSDSYNAFKEGTVSSGNACRPGSPLGRSSA
                     PIPISAPHNLTIKAGTTSRDDIIKDTKNGILISRLWYTYAVNPIKGDFSCTARSGNWI
                     IKDGQISTPAKSVRIIHSLPRLLQNISAIANNQRTVLSWAAMPVTAPSIRCENIPVVP
                     I"
     gene            13803..14147
                     /locus_tag="VGA92_08275"
     CDS             13803..14147
                     /locus_tag="VGA92_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846438.1"
                     /translation="MKARLTYVPLDIANHFDDFIIKRDEQILDAIKARSRDFSTLSLL
                     KLLYQLSNGPMTFSDLYTNSKIRMKRSFLNYLHLCVDYNFVTRKKLGQCMIYTISERG
                     RIMLNLFMHKGN"
     gene            14169..14894
                     /locus_tag="VGA92_08280"
     CDS             14169..14894
                     /locus_tag="VGA92_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX9846439.1"
                     /translation="MRQRASEDHVHEIKKINYQSPIIFAGFVGPGLAGPLSVGYLIQK
                     LQMQEVGYLRSKYLPPSTVFMQGRLRHPFRFYSNKKGTICAIICEMTLRMEGLYDIAS
                     TILDWAESKGTRELVILDGVASSEHDNKTFCAAEEDLCRIMSEKGISMISQGFITGIP
                     GSILNECLVRKIRAITLLVKANHASPDPLAAATLIEAINSVYGTGIDTSELRAEKEKI
                     GIEFKELSEKYKEHRQIDSGMYM"
     gene            14931..>15356
                     /locus_tag="VGA92_08285"
     CDS             14931..>15356
                     /locus_tag="VGA92_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX9846440.1"
                     /translation="MALTYKSAGVDVQKIKKSQSAIGKIISSTHKSQKVKSGFGHYAG
                     LVEIHGGMFLATHTDGVGTKVLIANMLKKYDTIGIDCVAMNVNDIICIGAKPISFVDY
                     IAANQNNEHIFTEIAKGLAAGAKKAAVPIVGGETAIMPDL"
BASE COUNT         4791 a         3120 c         3229 g         4216 t
ORIGIN      
        1 atttgccttg tcagttgaca catatgctcc tttttcacca gctgaatatt cttccgggtt
       61 ttcaacaact ttgaaataaa ctattgattg accaaactcc tttgaaaccg tcacacggta
      121 atctccatat ctgagattgt cgtttgggat ttgcagtttg tattcaaatg agctgtcgcc
      181 tgcaagattt acgtgattct ttattcggaa tgcgttttgt gtatcttttc caattgcggt
      241 agccccagac tgcaagacct ccaaatcaag tgcggcaaca aacttgttac ttctgccaga
      301 gattactacc gtctctccaa gtccgtaagc ctccttgtcc gtcgtcagaa caattttttc
      361 tgtgtctttg acatctttaa ccagctcaaa cgtggtttct gcatgttgtt taccatagtt
      421 tgcaacaata tcaaaagtcc caaagacagg atttacagtc gtcatataga ctatagttga
      481 gaattcgccc tttgttgtag gatacagatt tcctgagaag actcggtttc catttggatc
      541 aaagactccg tattccagac ccgttaatgg gattatttcg gttgtacttg ccgagatttc
      601 aactcgctgg ccaggaatgt aagcgacatt atcgctagat attgttagtt ctgattcttc
      661 ttccgttatg acagtgacag ccgtctcacc aaccgaaaat acagttttgt cttgcaccac
      721 atcataaacg acaaagacat cataagttcc aggacccact ccaagcacct tgtcaagctt
      781 tagtgatgtt ttgaattcaa ggttcagatc tggataaagt gtaattcttt tgtcatagct
      841 gcccggtcca tcaatgtgta ttttgatttg ttgctgtgaa aagtatggtt tttcctggta
      901 gattcgtttt gatacctttc ccgaaattgt tgccgtctca ccaaagtcgt aagactgttt
      961 gtctgtgcta acagaaatgg tcaggtcacc aggtcctgca gatactgtat caagttttcc
     1021 gtttgaagaa ctaacagaag atttcctaaa aacccaatca tttgacgcat cagaatcaac
     1081 tccatcaaaa tttctctgcc aagatgagaa atcgtttttc tgatcagtta tgacgggggt
     1141 ttcatcaatt acattgccaa agttgtcttg aagtcgtact ttttcagaaa catcggtaaa
     1201 ccacaaactc gcatatgaat aaaccaaaaa ctgacctgct ttgatttgag tcccagctgg
     1261 gattgttaat gtctttttta aaatggtagt tgatgcaact ttccacccgc caatatctac
     1321 tgtctcatca gtagggttgt agagctcgac ccattcagat attgcctttg agtcgtcgcc
     1381 aggcggattt gtgtcaactt cgttaattac gacatgatca gataattctt gagcataaga
     1441 gctagtaaaa actccaaaga acagtaaaga tgcaaataca ccagttagta accaaggcct
     1501 cattttttac ctataatttt gtcgttttta ttatcatttt agaacttgat tgagcaaaag
     1561 ttgagcaagt tgtgaatgtg ggcttcattt accctcaatt tgcgtcttct tcattaaaaa
     1621 atgatgcgca tcatttctct ttttgcccac ctgcgtcaca atttggacaa tttccgctaa
     1681 tgattttggc gccacactcc acacaaacgc cctctggaat ttctttttct attagtcgtt
     1741 gtcgccgccc cacaaagact ttgattccaa catagatgcc aactgcaatt attacggaat
     1801 agattaaagg caatccggct cccagcagac caatcatgaa aaacaggact cctgcaacta
     1861 ggaggatctc ttttttttgg tagctcaatt acgtttgcat tagtagagtc aattaaaaat
     1921 cgttggattt aataaaaatg ggattggccg gatttgaacc gacgacctca ggtacccaaa
     1981 acccgaatca taccaagcta gaccacaatc ccaatacggg atgcagcttt tgcaccccaa
     2041 tttaagccac atgattttaa ttaagatgta aaatcaatgc aaaccattgc aaatcgatga
     2101 ttatgtcaaa gcaggtaaaa ttgcatccga ggtcagagaa cttgcaagag caaaggactg
     2161 gacaggaaaa accctctacg agatctgcga aactgtcgaa tctgaaatta agagccgcgg
     2221 tggaaaatgc gcatttcctg taaatgcaag cttaaatgaa tttgcagcac attacactgc
     2281 tgaaccagat gatccaaagg ttttgtcaga cactgaccta atcaaaattg atcttggagt
     2341 gcagataaac gggttcattg cggacactgc tgtcaccgtt tcatacgacc ctgaatttga
     2401 agtgctagta gacgcagccg aagaggcgct aaaaaacgca atggcgatgg taaaggaggg
     2461 ggtcaaagcc agcgacattg gtaggactat tgaaaaaaca gtaaagcagt acgggttttt
     2521 gccaattgca aatcttagtg gccactcact tgaacaatac acgattcatg ccggaaaatc
     2581 gattccaaat atttggtcaa ttggctcatt ttcactttct tccaaagaag cgtatgcgtg
     2641 tgagccgttc gttacgacat caaaaggctt gggttttgtg cgggaaggca agatacgaaa
     2701 tatctttgga ctcatatctc ggaaaaaaat taaaaacgat gctgcaaaca agcttcttga
     2761 ttacatctgg gaaaacttta acacgctacc atttgcactt cgttggatca ctaaggaatg
     2821 gagccaaaaa gaagcaaagc ccctgcttga cgaattgatc aaaaacaaag ttgttcgcgc
     2881 atatccggtt ttgattgaag caaacgagca aagggtcgcc caagcagaac acaccttcat
     2941 accaactgaa actggtgcaa tagttactac cctataggtt cgccaaagat tttttcaata
     3001 accattggaa ccttacaaga aacgcattca gtacttggcg caaaaagaaa atcgccttct
     3061 ttgaactttc ttttcaattc atttttgcat gatgggcatt tttcagcagt atacgcaatg
     3121 atttgttttt ccttttttga aaatatcatt gtgacactcc caacgtgttt ccaacaccaa
     3181 ctacaagaat attttgccca ggtgccgtat tttctctaat cgtttggtag atttgggatt
     3241 cgacttcttt tgccttatcc gcaatctcct ttgtcataag tgtaattgct tcttttatcg
     3301 attgttttat gacgattgca taaaccggaa tgttgttctt tgttgcaact tcttcgattt
     3361 ggaatctgtc tgtcccaatt ccgccaattg cagctccaaa tccctgagca atggctccag
     3421 agtcttcgcc ttcaagtttt aacgctgcat ccaccatgac gattatgttt ggtttttttt
     3481 ctttaaccaa gatttctacg gcatcgcctg gcctcccaac tgtcgcagct ggcccctctg
     3541 cttttaaaag gaatagtttt ctttgctcaa agtccatttc tccgtacact gtctcaaacg
     3601 taattgtctt tttttgcgtt ccaagcatca atttgcctac aaccattggg ccaataccat
     3661 cacccattgg ctgaccttgt tttaatgccg gtacggcatc ctttagcgca tctgcctgct
     3721 ccattataaa tggcatcatc atttgtaatg gcaaaataag tggaaagttg ttttgctttt
     3781 ttgcagtaag atagaggtgt ctgactattt tgtgtaacat gtgtagtgat gtaactactt
     3841 ccaaaatgtt ttggatcttg cttagctcaa gcgcattcat acttggcgag agtgatttta
     3901 cttgtgctct tgtatagtct tctcttgacc taacaagatg ttttattttt gggacaatac
     3961 cgtttgggtc aacatcgact ggcattatgg taaagtattc caggaattgc tcaacttttt
     4021 ttggagcctc gtctgttgtg tttaggtttt ttttcagata gtctaaaagc ccctttttag
     4081 aatcttcttt gtattttttt agcttttcta tatttttgtt aatttccccc gatgtaattt
     4141 taagttggat tctttgccca taaaacatga agattacgat cggaataatc cagacaagcc
     4201 acatcagtgg gttgccggtt tccccttcga aaagggtgtc aggcgataac atctctgtaa
     4261 agttcaacta gtggatgggc tgattagacc aaattaattg atttgtgtgc atggatcata
     4321 attggctgtg caaatttcaa agaaattgtg ctcctagtaa acaattagaa agtgcttttt
     4381 atataggcta gctgagtcaa ctggtagcat atgccccaaa caaagcccat agtaagcata
     4441 gagaatgtgg ttgcttctgc ttcagtagat caaaaaatgg acctaaatga gattacccgt
     4501 aattttccag atgtagaata ccacccagac cagttcccag gacttgtgtt cagactaaag
     4561 agtcccaaga ctgcaacttt gatttttact tctggcaaga tggtttgcac gggagcaaaa
     4621 tcagaagaaa tggcaaggag tgcagtaaaa tcagtcgttc aaaagctcag aaagggcgga
     4681 atcaaggtca aaaaggacgc agtagtggat attcaaaaca ttgttgcatc aattaatctt
     4741 ggtggcaaaa ttcatcttga gcaggcagca aggacgcttc ctcgaagcat gtacgaacca
     4801 gaacagttcc caggcttgat tcacagaatg cttgatccaa aaactgtgat tttgcttttc
     4861 tcttcaggaa agcttgtctg cacaggcgca aaaaaagagc ccgatgtata caggtctgtg
     4921 aacaatttgc attcgcttct tgaagaaaaa ggtttaatga tttacgatta gactttcttt
     4981 tttctaataa gttagaagta aagcgaatat atcaacacaa agccacaaag tgtataagat
     5041 gaaaatagat ctttttggag atttcaagct aaaagacatt gttttggttg catccctgcc
     5101 agatatgggc agggttggag gtttggtatc agcacacatt gcaaaaaaaa ctttggcaaa
     5161 agacgccgcc aaagtggttt tgtcagacaa gccctgggtc aaccaagtag acggtcttgt
     5221 cgaggtaccg tttgatgagt ataagatttt agttgatgag aaaaactcgc ttgttatttt
     5281 tactggtgaa aaccagcctc aggaaccaaa tgcagttttt gagttggtag attttgtaat
     5341 tgatacggtt caaaaatttg gcaacatacg attgataatt tcaactgggg gctatttgcc
     5401 attacaaaaa atagatgggg atcaggtata tggtgttgcc acagaccaaa aacttctaga
     5461 gtcgttaaaa ccacacgaca taaaaacact atcaaatgat gtgaaaagta ttacgtggtt
     5521 taacggcctt gtcttgggcg ctgcaaaaaa cagaaacatt gacggaatcg ggttgtttgg
     5581 cgaaatcata gaccccgagg caccacaaca cagggctgca aaaaacatca taaacaaaat
     5641 tgaaaaaatt cttaagattc agataaacac tgatgaattg caaattgctg aaaagtcgcc
     5701 tgagctcaaa aaagatgggc ctggaatagg ataatcattt tatttttgtt ccaagtggaa
     5761 ttgattcatc aactgttaac cagtagggct tgtcgtttag gtctgctgcc aaaagcattg
     5821 cgtttgattc cacgccacca acagtttttg gttcaagatt agttactgca ataacagttt
     5881 tccccacaag ttcttctggt tcaaaaaact gtgcgccgcc aattatgagg ttgcgcgttt
     5941 cactgccaag gtcgattgtt cctttgatta tctttgtttt gcctggaatc ttttctgtcg
     6001 agattatttt ggccacccga atctcgagct ttgcaaaatc atcatacgtg attgtcatag
     6061 caaggctttt tttctctcgt taaaattaat ttcgaacttt agtgatttca ataacgtttg
     6121 tgtactaagg ttaacagaga tcaatttatg taaatgtctt gcaaggaata tcatatgggc
     6181 cagtttttta cagacgacat tgacgccctc gtaaaggaag gccatgggga tcatgagcgc
     6241 cttgtgagaa tcaagtctga ctttgaggca aaaaagcttg ttacacttga agaccgaagg
     6301 tatgttgaag gcctagtttc gcggtatttg cagccaagca aaccagaatc ggaaagaatt
     6361 gtcaaaattc cagaaaaacg aattgtcccc ccgccttcac caccttcaca aaacatttca
     6421 gttgagatga agtatcaaaa acaaaagagc caagaaccga ttccaaagat aggaggaaaa
     6481 agaaacatcc gaaatgtagt agttagtgtc gctgtagttg ttgcagctct tctgataatc
     6541 agcgttgttg caataaatcg cgacaatctc ggaattgacc agccagcagt aactgaaaac
     6601 atagagcttg atcaaacatc ctatgcccga ggcgacatca tctcaatttc aggcaaggca
     6661 actaccagca caacagtaca actcgtaata agcaatccaa gcaaccagca aatctggagc
     6721 gagacggtaa gtacaaaggc aaatggagag ttttctactt tgaccattgc cggtggaaat
     6781 ggttgggagc agtcaggcag ctacacatta agctcgactc atggtggcac aacagaacaa
     6841 gtcacatttg agtttacaaa ctagatttaa agactaatat ttcagctaca aagatttcca
     6901 aaacatggcg acaatagagg tctcaactat gataaatgca ccagttgaca aagtttggga
     6961 aattgtctca gatatagaca atgagccaaa gttttggaag ggaacaaaac agattcgaaa
     7021 catctcaaaa aatgataatg tggtaacgcg cgaagtgaca attgcattta aggattccaa
     7081 atgtatgcag acagtgacgc tgtacccaaa agaaaagatt gtcggacaat ttactgaggg
     7141 catactaaat ggcaccaaaa ccataaaact cacaccccaa gaaaacggca cgaatcttga
     7201 ggtagtctgg gacatcaaac tatcaggcat gatgggcatg tttacaggca tgatcaaaaa
     7261 gcacataaaa aatggaaccg agcaggcact agaatcaata aagcaagaaa tcgagaaatg
     7321 acatggattt tgtcgatgta atcaattatg ccttttttgc cattttgatt ggggtagccg
     7381 gtgcatgggc gttactcata aaatcgatga tggagtcgtt taaaaaaacg ccgtttcttg
     7441 acagattcaa agcaaaagaa cacacacacc ccaaggtttc tgtaattttg cctgcaagaa
     7501 atgaagaagg attcattgaa aagtgtcttg actccttgct gaaccaagat tatgataatt
     7561 atgaaatcat tgcaatcgat gactcatcag aagacgcgac tggtagaatc attgcagagt
     7621 ttgcaaggaa aaatccaaag gtaattcatg ttagtgccag accaaaaccg caaggatgga
     7681 tggggaagaa ctgggcatgc atggaaggat ataaaaaagc aactggcgat cttttacttt
     7741 ttactgattc tgatacgact cattcaaaaa atgtaatctc gttagcagta tcgcatctat
     7801 tatcacttga gcttgacgcg ctgacagcca tcccaaaaat gatctgcctt gacacttgga
     7861 caaagataac cctgccagtt ctctccactt ttttgcacac aagattttct gcacttcggg
     7921 taaatgattc ttcaaagaaa acagggtatt tttttggaag tttttatctg atcaagcgtt
     7981 ccgtgtatga tgctgtaggt actcatgaag gaataaaaca ggaaataatt gaggatggtg
     8041 cacttggaaa aaaagtaaaa gagtctggct atagaatgcg aatggttcgt ggcgaccacc
     8101 taatcgacgc agtctgggct cgcgattggt ctactttgtg gaatggtcta aagcgactaa
     8161 tgatcccact ttatcttcaa aacagcaaaa ttgcagttgg catatttttt gcagtcttgt
     8221 ttttgctttt catgccgtac gttcttcttg catattctgc tgcctttttc tatcactcgc
     8281 agtcattttc tgcgcttttt tgtgcagctc ttgcctcagc agggctgctt tatgctgcag
     8341 ggcttcttga tgcaagggcc ttaggcatca aggcgccaca tggaatttgc ggtccaattg
     8401 ggagttttgt tgtagttgca ggatttctat gtggaatttt gcaggcaaaa agcaacatgg
     8461 ctgtttcttg gcgaggtcga gcttactcta tgaaagacca tgcccaaaac tcgatcagtg
     8521 tgtagcgagt aacaattata gattatctgc aaactgccaa gttattgagc aagtcgaccg
     8581 taattgtcag cagtctgtta gcaacagtca ttgtgcttgt agcatacagt atttttctca
     8641 atccaccaga taacatccct atagtagacg aaatttcaaa atcgacaaag aacaccgtga
     8701 ttgcccaaga caaatccatc ctagcttctc aagaccttcc tctcacagaa atttttgaaa
     8761 agacagaatc gggtgttgta agaatcaacg taaagagacc agacacagat tcacgtggtg
     8821 ctggtggcat tggctccgga tttgtctatg atgattcagg acacatcata acaaatgatc
     8881 atgttgttac aaattcagaa aaaattgttg ttacgttttt ggacgggaga tcatttaatg
     8941 cggaaattgt gggcaaggat gtctttactg accttgcagt cataaaggtg aatacagatg
     9001 cagacgcatt gcatccatta ccgcttggcg attcttctaa gcttagggtt ggagagcaaa
     9061 ttgctgcgat tggcaatccg tttggccttt ctggctcgat gacgtcaggc attgtaagcc
     9121 agcttggaag actgctccca tcacaagacc gaagttttca aattccagat ataattcaga
     9181 ctgacgctgc cataaaccct ggaaattctg gtggacctct tttgaacatg cgaggtgaag
     9241 tcattggaat aaacactgca atccaatctg agagtggtga gtttgcagga gttggctttg
     9301 ccataccttc aagaactgtt caaaaaatta ttcctacatt aattgaaagt gaggtatatc
     9361 atcacccgtg ggtaggaata tctggaaggg atatagaccc agacttggcc aaagtactag
     9421 gcctgcagga cgcaaggggg tttctgataa tcaacgtgtt aaatgacagc cctgcagaaa
     9481 agactgggct taggggtagc actgagataa aagaaataga tgacacaagt taccaagtcg
     9541 gtggagacgt catactttca gtcgatggga agattgtaag aaaaatcgat gacattttaa
     9601 ttcaccttca acgcgaaaaa tctgttggtg accaaatggt cttgcaaatt ctccgtgacg
     9661 gcaaagtcac agatgtgatc ataactttgg aggaaaggcc gaactcaaac attacaaata
     9721 attccaccaa ttcaacacaa aaataaatac tacaaaccaa aagtaacgac agattgagcg
     9781 atcttgtact tggctgcgag ccactaaatc gtgttcttga tggaaaagaa atcacagcgt
     9841 ctgatgcata tgaaattttt cgtttgtcat caaataatcc cttggaggtc tacaagacgg
     9901 ctgcaatact tcgcaataaa cataaaaaaa gagtcgtcac gttttcaaaa aaggtgtttt
     9961 tcaatttagt taatctttgc agcgatgttt gtggttattg cacgtacaag tcagagccag
    10021 gccagcccaa gctctcactg atgagtaaaa aagatgtgca aaatctggca aagcttgcaa
    10081 aaaagcatcg ttgtgttgaa gcgctttttg tgacaggtga aagaccagag caaaaatatg
    10141 aagaggcgca aacatggctc aaagaaaatg ggttttcaag cacgccagaa tatctcgccc
    10201 atgcatcaga gattgcactc tcagaaggtc tattccccca cacaaatgcc ggcaacctca
    10261 caaaaaatga gatcaatgat cttaagaaaa caaatgtcag catgggccta atgcttgaga
    10321 gctccagtga gcgcctaacg gaaaaaaata tgccgcacca tcttgcccca agtaaaaacc
    10381 caaaaacgcg gctgcgcgtc ttggagtctg ccggggagct aaaaatgcca atgactacag
    10441 gattgcttgt tggaatcggc gagactgcac atgagatcat tgactccatt ttcgagataa
    10501 aaaaactgca ccaaagatat gggcacatac aagaagtcat tttacaaaac ttccagccaa
    10561 aaccagatac gcttatgagg ggtattgcat cggcagagga aagttatttc aagacggtag
    10621 tagcactcac cagagtgata atgccgcaaa tgaacatcca gattcctcca aacctttccc
    10681 caaactctta tcagagcttt atcgaaacgg gaataaatga ctggggtggg atatccccgc
    10741 taacgccaga ctatgtgaat cctgaattcg gctggcctga aattgagttt gtgcaaaaaa
    10801 acacgaataa tgcgggtttt gagcttaaag caagatttcc agtttatcca gaatttatgg
    10861 gccttgtaag caccgagctt tgtgataaaa tggcacaaat tatggacgag gaaggtcttg
    10921 taagcgagga atactggaaa tgacatcagt tatcgagtcg ttgttaaaaa attctgatcc
    10981 gttaattgcc gagacgcttg atagggcact atcagaaaaa gagatttctg taaaagaagc
    11041 aacaagactt tatgatgcct ccggacctga ctttcatcta attggcatgg ttgcagacga
    11101 gcttagaaaa aggcgagtcg gagacattgt cacatttgta gtaaacagaa acatcaattt
    11161 tacaaatgtc tgcataaagc aatgtggctt ttgtgcattt agcagggatt ttagagagga
    11221 agagggatat tttttgccga cagaggaaat tgtccgtcgt gcaaaagagg ctcatggttt
    11281 tggtgcaacc gaagtatgca tccaggcagg tcttccccca gacatggatg gaggcttgta
    11341 cgaaaacatt tgcaaagcaa tcaaagccga gattccggac attcacatac atggattttc
    11401 cccagaagag gtattatacg gtgcaagaca gtcgggtttt tcaataaaag aatatttgca
    11461 aagactaaag gcagcagggg tcaacactct ccctggaact gcggcagaaa tacttgacca
    11521 agaaattcgc gacaagattt cgcctggaag aattagtgtc aatgactggg ttgacatcat
    11581 caaaactgca cacaagcttg gaataagatc gacttcgacc atcatgtttg ggcacataga
    11641 gactccagaa gacagagtaa agcacattgc caagattaga gaaattcaaa aagaaactgg
    11701 cggctttaca gagtttgttc cactcaactt tatccataca gaagcaccga tgtacaagca
    11761 cggactacat gatgggattc gtagtggtgc gggtgcaaac gatgtcatgt taacacacgc
    11821 cattgcaaga ataatgctca acaactacat agataacatc cagatgtcgt gggtaaagga
    11881 aggcccaaag atgtcgcagc tcttgctcaa atggggcgca aatgactttg gcggaactct
    11941 catcaacgag agtatatcaa cgtctgcagg agcacagcac ggtcagatac taagaccaaa
    12001 agaaattaga catctaattc gcgaggttgg gagaattcct gcacagaggt caactacata
    12061 caagattctc aaagtctacg actctgattc ggattacgag gatcttgaca aagtggagga
    12121 catttcacgc tttggttctt attttgagct tgtaaaaata gacaagtaca gatacaagaa
    12181 cccaagggca aactaggctt gtctgtttgc gacgatgtaa tttcttgtgc aaaaaaactc
    12241 ggcattgatg agtgcgagtc tgtagtttgt gctaggaaaa taatcacagt cagaatcaca
    12301 gattccgaaa tcgccgaaat taaggaaaac tatgagaaat ccttaggaat tcgtctcata
    12361 catggcaaaa gaatctcttc ttgccaatca acaattcttg agccggtaaa gctagtcgat
    12421 gccgcattca ggtcatccca aaacctaacc aaaagagatt tttggaagtc cttgcctgcc
    12481 gggacaaaaa ccgacgtgaa catccaaaag acaaatgacc aaaagatttg gcagctagag
    12541 tcttctggtg cagcagacat tgcacaacag atgattaatt ctgcatctca ccaaaagata
    12601 actcgaattt ctggctctct gaacatagtt tgcgaggagt ttgaactaca aaatacaagc
    12661 cagttgaata aacacgacaa ggcaacctat gtttcaggca taataaacgc agattctgaa
    12721 catggaattc ctgtgagcgg catcgggcag gcaagctcta ggactctagc agactttgat
    12781 gcagaacggg tcggcactga agctgctcaa atgtgtgtaa attcgataaa tccgcaaagc
    12841 tgtgaggctg gaacaagcac cataattttt gaaccattgg cagtaggcga gcttttatca
    12901 tttgttttta caccgaactt tagtctgaag acatattctg agaagcgcag ttgcttttca
    12961 gaaaaaattg gtgcgcagat atcagttgcc gattttagct tacttgatga cccgcatgca
    13021 acaaacagat tgggaagcaa gtcctttgat gatgagggaa ccccaaccaa agtaacgcaa
    13081 tacgtccaca atggaatctt tgagaacaca tactctgatt cctacaatgc gttcaaggaa
    13141 ggaacagttt cttctggtaa tgcgtgcagg ccgggctctc cacttggtcg cagttctgca
    13201 ccgattccga tttcagctcc acacaacctt accatcaagg caggaactac aagtcgagat
    13261 gacatcatca aagacacaaa aaacggaatt ttgatcagca ggctttggta tacgtatgca
    13321 gtcaatccga taaaagggga tttttcatgt actgcaagga gtggaaattg gatcatcaaa
    13381 gacggtcaga ttagcactcc agcaaaatct gttaggatca tacacagcct tcccaggtta
    13441 ttgcaaaaca tttcagctat tgcaaacaat caaagaactg ttttgtcatg ggccgcaatg
    13501 ccagtcactg ccccatcaat tagatgtgag aacattcctg ttgttccaat ctagcatgct
    13561 taagtatcat tgaatcgtaa taatgacatg tgtgaaactc tggacgagat tcattccatg
    13621 cagcttgcat caataattgc aaagatgcga aaaacaaaga aagaggaaaa acagctaccg
    13681 gccgtagcat aattcctcac tttttgttta attcacgcct gcattgttta aaattattgc
    13741 cagaaactca actaatttac aggccaatgt taataaggta atgtaccgta ccatacggta
    13801 acatgaaagc acgactcacg tacgtaccgc tagacatagc taaccacttt gatgacttta
    13861 tcataaaaag agacgagcag attctagatg caataaaggc acggtctcgt gactttagca
    13921 cactgtcact tttgaaactt ttgtatcaac ttagcaacgg cccgatgacg ttttctgatc
    13981 tttacacaaa ctcaaagatc cgcatgaaga gatcgttttt gaactatctg catctttgcg
    14041 ttgattataa ttttgtgaca cgaaaaaaac tcggccaatg catgatctac accatttccg
    14101 aaagaggaag aatcatgcta aacttgttca tgcataaagg taattagtgg cagaaaaaac
    14161 cgaacatagt gcgacagcgt gcctcagaag accacgtcca tgaaataaaa aagatcaact
    14221 accaaagtcc tataattttt gccggttttg ttggtcctgg ccttgcgggt ccactttccg
    14281 tcggatacct aattcaaaaa ttacaaatgc aagaggttgg atatctgcgc tcaaagtatt
    14341 tgccgccatc aactgttttc atgcaaggaa ggctacgaca cccgtttcga ttttactcta
    14401 acaaaaaggg aaccatttgc gcaataattt gtgaaatgac actaagaatg gaaggattgt
    14461 atgatatcgc atccacaatt ttagattggg cagaaagcaa aggcacgaga gaattggtaa
    14521 tcttagatgg tgttgcaagc agcgagcatg acaataagac attttgtgca gcagaagaag
    14581 acttgtgtag aataatgtct gaaaaaggaa taagcatgat ttctcagggg ttcataactg
    14641 gaatcccagg aagcatccta aatgagtgcc ttgtgcgtaa aattcgcgca atcacgctac
    14701 ttgtgaaggc aaaccatgca agtccagacc cccttgcagc agccacgtta attgaagcga
    14761 taaattctgt ttatgggact ggaattgaca catcggagct acgagctgaa aaagaaaaaa
    14821 ttggaatcga gttcaaggag ctttcagaaa aatacaaaga gcacagacag atagattctg
    14881 gcatgtacat gtaaaagttc ttttatgcag cccacaaacg caattatgtc atggcactga
    14941 catacaagtc cgcaggagtc gacgtacaaa agataaagaa aagccaatct gccattggaa
    15001 aaataatttc atcgacccac aagtcacaaa aagtcaagtc cggatttgga cattacgcag
    15061 gcctagttga gattcacggc ggcatgtttt tggcaacaca cacagatggt gtcggcacca
    15121 aggttctgat tgcaaacatg ctgaaaaaat acgacacgat tggcatagac tgtgtcgcga
    15181 tgaatgtaaa tgacatcatt tgcattggcg caaaaccaat ttcctttgtt gattacattg
    15241 cggcaaatca aaacaacgaa catattttta cagaaatagc aaaaggcctt gccgcaggtg
    15301 caaaaaaggc agccgtgcca atagttggtg gggaaactgc aataatgccc gacttg
//
LOCUS       DASWKH010000110        26533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3250823, whole genome shotgun sequence.
ACCESSION   DASWKH010000110 DASWKH010000000
VERSION     DASWKH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 26533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26533
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250823"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..280
                     /locus_tag="VGA92_08290"
     CDS             74..280
                     /locus_tag="VGA92_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846441.1"
                     /translation="MWGIIPEENRQIRCPCGHTEDRNISAAQNILYKGIQVQLRVLRL
                     RPDAPQGEAMKQLKDAKQIVVSYL"
     gene            378..638
                     /locus_tag="VGA92_08295"
     CDS             378..638
                     /locus_tag="VGA92_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9846442.1"
                     /translation="MQIVADLLTATDHCGQEGIKVTTLLTQANLSHSRLSKFIENLTG
                     AGLINKIEFDGKNTFVITPKGKQYLESYKRFHDLAGSFGLDL"
     gene            complement(635..820)
                     /locus_tag="VGA92_08300"
     CDS             complement(635..820)
                     /locus_tag="VGA92_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846443.1"
                     /translation="MDKYLVIVFTLLVGGMIFSIMKDPPSLELFYGQLAGAIIIVIYS
                     SFKNRKSHQSAKKKRKD"
     gene            871..1032
                     /locus_tag="VGA92_08305"
     CDS             871..1032
                     /locus_tag="VGA92_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846444.1"
                     /translation="MSKVLELIGFGLIGGTIYAWIGMIDNPMRWPIFWGCAGGALAII
                     LYRKFKKRK"
     gene            complement(1226..1299)
                     /locus_tag="VGA92_08310"
     tRNA            complement(1226..1299)
                     /locus_tag="VGA92_08310"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Thr,seq:cgt)
     gene            1352..2644
                     /locus_tag="VGA92_08315"
     CDS             1352..2644
                     /locus_tag="VGA92_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEX9846445.1"
                     /translation="MKKSISGIRGIFGNDLTLKDILKFCRNFTPLIKSDRCVVGRDTR
                     PSGEIIKETAMAALMGQGISVYNLGMVPTPVVFREARKYGAGLIITSSHNPLEWNGLK
                     FIIEGRGPNEKEFAQILKEKKSNNKKIGTETEVSSNYVDDAARIIGKINQKPKIVIDV
                     GGGAAVNTAPHLLQSLGCQVQTINQNSSKRGPDPTSDSLSELVSMSKNADIGFAFDLD
                     GDRLVVVKDGKKQSPDTTLALGVSKALELGYKKFCLSIDTSTSVEKYILDHGGRVIRS
                     KVGEANVIDVMIRQKCQAGGEGSSGGFILPEFNMCRDGILSAGLIAAMTGKKIIDQVI
                     EFVSRYHQLRTKIGIESNLHDKVLDRFERNLKGKFSQIITIDGVKAIIDDDTWALVRK
                     SNTEDIVRISVESNNLQKAKQIQKEITSLVRKSHDQTR"
     gene            2628..3581
                     /gene="thiL"
                     /locus_tag="VGA92_08320"
     CDS             2628..3581
                     /gene="thiL"
                     /locus_tag="VGA92_08320"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX9846446.1"
                     /translation="MTKLDERQIIKIFQRNFSGKKFVSEDVEVFRIGKSFGVIKTDTL
                     VESTDVPPGMSPAQIARKSIVASVSDFAAKGVRPLYCVVSVSLPRNYSKSKVLQLASG
                     LKNASREFGFRILGGDTNEGKELVISATLFGLAKKIARRAGAKAGDHIIVTGPFGRTS
                     AGLKIVLHGKKASAGFKKLAKKAVFLPTPRLIFGVSAATYMTSAMDSSDGLSTTLNEM
                     AKQSNKRFVITQIPKDDRLDSFAKDNKLDLIDLVFNGGEEYEIVATASPKDLNRIKKI
                     ARTKKIRLICIGRVQKGAGVFLQKKQKLVKIRDRGWLHFTN"
     gene            3654..4052
                     /locus_tag="VGA92_08325"
     CDS             3654..4052
                     /locus_tag="VGA92_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9846447.1"
                     /translation="MSEERVEKWGVAHIYSSYNNTIVHMTDLTGAETVAISSGGVHVT
                     ADRYESSPFAAMKSSNAVVEAARTKGFTGFHIRVRAVGGVGSRVPGPGAQAAIRALAR
                     GGFKIGRIDDVTPIPHDTTRKKGGKRGRRV"
     gene            complement(4049..4366)
                     /locus_tag="VGA92_08330"
     CDS             complement(4049..4366)
                     /locus_tag="VGA92_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846448.1"
                     /translation="MIQWVFEISSTDYLEDKRLINNALSDIAAQCGLQNEFEFGSPVS
                     RFGWTFFKLWIKTNLQLQINGKFSDMIKKTKGSKSEEKFTNFLSDYFQSQGCKAKIKL
                     IEV"
     gene            complement(4347..4769)
                     /locus_tag="VGA92_08335"
     CDS             complement(4347..4769)
                     /locus_tag="VGA92_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9846449.1"
                     /translation="MSDEDKELEMLKAKRLAEMKKNVFSQTQKDQQKPTEQKPPSYRD
                     IVIGRLGYRGMEVLQNAERQYPNDTKLVIEKLGQLIHSGEINEEIDGGKLLALFRSVG
                     INVRMETKINVEQDGKFVSLSDKLSRATESEYNDSVGL"
     gene            complement(4766..6214)
                     /locus_tag="VGA92_08340"
     CDS             complement(4766..6214)
                     /locus_tag="VGA92_08340"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX9846450.1"
                     /translation="MSSISPKKIGNMEYRIDADSSKGMRVPVTIYADEGLLSKMMTDR
                     TIWQAVNVSTLPGILGHAVVLPDGHEGYGFPVGGVAAMDAQEGVISPGGVGYDINCGV
                     RLLRTNLSEKDVRPKLKELVTDLFNSIPSGVGSEGAIKLSYSQLDEVLVRGVNWAIEH
                     GYGTHDDADVCEENGQIKNADPNKVSNTARKRGAPQLGSLGSGNHFLEVQRIEKIYDE
                     EAAKRMGIAEGNVTVLIHCGSRGFGHQICSDYLRISEQALRKYDINLPDRELACVPNT
                     SEEGESYRKAMFAALNFAWSNRQMITHWTRKSFERVFKKTESDLEMKLVYDVAHNIAK
                     VEKHKIEGKERPVVVHRKGATRAFPANREEIPTKYRDLGQPVLIPGSMGTGSWILLGK
                     PNSMSLSFGSTAHGAGRMMSRSRARREYTEEQVKKSLHDKGIFIKSLTRDGVVEETPE
                     AYKDVDAVVNVSHELGIATKVAKLVPIGVIKG"
     gene            complement(6328..6624)
                     /locus_tag="VGA92_08345"
     CDS             complement(6328..6624)
                     /locus_tag="VGA92_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846451.1"
                     /translation="MKIIKVTALAAILATALIFANSSPAEAKAANTDDKLSPKSFGLK
                     SNQKLSIEKTYSDKFDPKKQLTKPDKLKPEQLKTKLKMIEAQKALEFAKKKYGI"
     gene            complement(6852..7439)
                     /locus_tag="VGA92_08350"
     CDS             complement(6852..7439)
                     /locus_tag="VGA92_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846452.1"
                     /translation="MSIINHKANKFGAFVGVLVLILAILVLPFNSVEAKVDETMCKSM
                     YERYKVLGEQKFREKYKNKSFLNDCIKLYKNPNWYFVGKSKIDKHYEKLNALMQNTTE
                     EKKITVKILSSISIGADKYLVKFRACAEKSTIQKPSFLIKSQFEQYLGLSSKVLQVGK
                     CNDYHAQIKTKQAANIQIEYVYDLSKYQNIKTKVI"
     gene            complement(7894..8697)
                     /locus_tag="VGA92_08355"
     CDS             complement(7894..8697)
                     /locus_tag="VGA92_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9846453.1"
                     /translation="MKYIFPILVIFVFLLMFQNVSAAPVPLWVKNIAKWWAEGSITER
                     EFLNAIEYLVEKGYIVIEDTQEDEIVPISPETSTTKLLEKGVENIKEEDNEIAILYFN
                     EALKREPDNVKALVDKGIATARTGKLAEAKFIFDQAIKMGETKNNLDYRAIINAGIAI
                     SIYGNQTDAMVYFDKVIANADKVDQNTLYAAYVNKGIAYYEQNKYDEAIDLYDKALEI
                     NPGRLGAIVNKANALQETKRYDDALTYFEQAYKITNDPLRWKPTFVLVE"
     gene            9013..9855
                     /locus_tag="VGA92_08360"
     CDS             9013..9855
                     /locus_tag="VGA92_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846454.1"
                     /translation="MPSEIGVYGNLHSYKLYVLPTAKRLFGSYSFRRKATIKHHTNVQ
                     KMLEILALHGPLTTWGMAKVVVHNEVSGIRTKEKEYRRLLKGRKDRGKHSPGVLDVGL
                     VVVDGKNHDRAPSDLYRLSLHGILYCLDVLDLTNKEIDMMAKHYSPVLPWVFGKWEYL
                     KSIIGSDVYRLKTLANGIFLDNIQVTKMSRFPVFELLTYLSIKYQEYFEYINEEKLAD
                     QISCWFYTHLLISSGTKSSIDNTKWKKILADQEIKKWYHGFTGEAMRFYQERFNTIKK
                     LGKR"
     gene            complement(9858..10223)
                     /locus_tag="VGA92_08365"
     CDS             complement(9858..10223)
                     /locus_tag="VGA92_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9846455.1"
                     /translation="MSNKKTILLVDDDVDLLENTAFMIKGMGHDVFTAGDGEEGISKY
                     KDVRPNLTFMDIRMPKMDGYDAFFKIKQFDPNAKVVLITAYNQDEKKYLKAKSMSLLD
                     SISKPYSFEILEGLIEKYA"
     gene            complement(10272..12125)
                     /locus_tag="VGA92_08370"
     CDS             complement(10272..12125)
                     /locus_tag="VGA92_08370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9846456.1"
                     /translation="MKISHLLIMSITILFLVYAIHSFLTYDFTLKEAKNIALLRIQAQ
                     ANSIATDLDKTIYEKTAVFQSLTKLEQIQSAVIESNQKFDSSNLKEQESLIEVSESNK
                     KTPFLRGILENEISDELADFISQYGQEYDYDVVKELFVTNQYGANIALASGKSDYLQS
                     DEEWWQITKNKQNYIGPIEFDENYNDYVIAFAYPILDDSSDYVGTLRISVSLRLLLTD
                     FLNDADLLHLSKKSVILLDQNGNVIYENGKFFPTDAPKEYFPRLVTENGIFEYGPSNS
                     VFVSYAKSIGFRDFSGFDWIVVIEQESSVVDEFEELEKNYLFTTLVGIISAIMLGVIL
                     SYFVTTPLAKLSKLTALLGKGNFDAKIQKSKITEINSIIDSFGEMEVSLKKLFETEKR
                     LAEANARIKNERLTAIGELAASMAHDMKNPLGTIRSGMDIIKRNTSTDSNTEEVIQRM
                     NRAVSRMAHQVEDVLNFVRVTPLSLKPVSINAIIDSAIKSIEIPKTIQVIIQGDDVVV
                     TCDEKKMEIVFINLILNSVQAIGDTQGRIMIKIKKVNENAVIEIEDSGAGVPQKVISD
                     VFKPLVTTKQKGTGLGLASCKNIIEQHGGSIKFQNNPTIFTITIPLEAKTK"
     gene            12531..12977
                     /locus_tag="VGA92_08375"
     CDS             12531..12977
                     /locus_tag="VGA92_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846457.1"
                     /translation="MIKLIGISILAVLLVVAIASLHPTDAITYEKQEDSKYVKQKIVE
                     PLKGKNGWWKYLLKVCADDRNLGITEVVLSSDMETIYQGVNKGLAKGKCTYFGAVMKA
                     KDGNTLGYKITQTHEAVEKIVESKQVKLSSSSWSEIMRYKFILGFY"
     gene            13428..14390
                     /locus_tag="VGA92_08380"
     CDS             13428..14390
                     /locus_tag="VGA92_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846458.1"
                     /translation="MKLTISLLLVVIVTLFSANALAQPYILLDKPTYSYGNTIKVSGS
                     VEYQEGMFIIIQIRSPSDIVAIDQIFPSRSGKFSAVFDTEGPKWGETGTYTVLVSYQG
                     QKTERTFQFSKPQPSPTETPAVQEPPTPPKPKITIKGFPDPTKSPQYYYERYNDDVEF
                     KRWFDSTFVGYTIQDVVGYKPTRVAGFPDPNQPPQYYIDRYNNEKPFMDWFDQYFSEM
                     TIYDVVGVTEQIRTIAPSWVKQYAGMWSNEEIDDERFIEGIADLIRQNIIEVNADITK
                     QDNPDKTIPVWFKRNASWYSQDLISEDAFLSGIQYLIEKEIIVV"
     gene            14458..15819
                     /gene="purB"
                     /locus_tag="VGA92_08385"
     CDS             14458..15819
                     /gene="purB"
                     /locus_tag="VGA92_08385"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX9846459.1"
                     /translation="MPILPIDGGRYGTKEMLAIFNDQKKIDYQLQIEGAAAVAQGQIG
                     IIPKSAAQNIFAVSKSGKITVKRIKHLEAKSDHDTAALVEALSEKCKPQARPWVHYGL
                     TSNDLVDTSTSMQMKDSLVIIEPKVARLALLLTKKAMEYKDVPAVGRTHGQHASIISF
                     GLKFANWASEMAKHIERIEEKKKRILICKTLGVVGTGSLMGVKALEVQKRVAKKLGLF
                     PAEVTTQIIPRERHAEYIFLLALIGSTLEKISIEIRNLQRTEIGEMSEHFKEGQMGSS
                     AVPVKRNPIKSERISSLSKLLRSQMSASFENIPLWHERDLSNSANERFTIPMCSILLD
                     EMIETMIRVIEKLKVDVEKIEQNLYITKGQIFAEFVLEALIKKGVPRFEAYRDVQRVA
                     FEAHASKEDYIDAVKGDKVMSSKLTEDEIDKIFSPSSHLGASSQIITNVNRTVQKVCK
                     RFL"
     gene            complement(16034..16789)
                     /locus_tag="VGA92_08390"
     CDS             complement(16034..16789)
                     /locus_tag="VGA92_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9846460.1"
                     /translation="MRQIDTLLEKVSNAISQTITGKKIGVAFSGGVDSSVIAKICSDM
                     NYDVTLLTIGFAGSHDIEFSKQVNEILRLPHKILEINPNEFDKISTSIKSKIKTDNLS
                     WNENSIAFYHVAKLAKSLGLNVVVTANGIDELFCGYNAYRDAIKGGEQVVMELMNSKL
                     ENELNMMRAVNEVCSEFGVNIVQPLLSDDFINYAKSVPISDKIKDENDLQRKHIIRKL
                     ALQIGVPEISASKRKKALQYGSLIHKTLLKNKP"
     gene            16979..17197
                     /locus_tag="VGA92_08395"
     CDS             16979..17197
                     /locus_tag="VGA92_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846461.1"
                     /translation="MSTIQIERQWQPEPKKNLVKSFPVKEGAKIYGQISAAKCVICST
                     PKVPYVFGQGKTPLVVICPKCGHKSWAD"
     gene            17480..17764
                     /locus_tag="VGA92_08400"
     CDS             17480..17764
                     /locus_tag="VGA92_08400"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9846462.1"
                     /translation="MGPAKLSDEQIKSELANLSGWSLKDGKLHKDFVFGDFIEAFGFM
                     TKASLHIEKMNHHPEWFNVYNKISVDLMTHDAGGITQNDINLAKTLNSLQ"
     gene            17812..18636
                     /locus_tag="VGA92_08405"
     CDS             17812..18636
                     /locus_tag="VGA92_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9846463.1"
                     /translation="MTTNPAILDADFRYIDKKGTLLKSRTELSVSQMLSFLGIDYDYQ
                     HKVILPGGKEFLIDFKTDDGKLIEVIDSDQDIDKYKQVKQFLPDAKVMAVGHPKFAAK
                     IKELDDVVFYDTKDAQSGSIFIEDPSFAFDYAHILPLVEKCSILHGHTSSVLVELVGD
                     MKNNLLIDFGEAKKIIKEIISILDHKFFINRKYLKSEDPSHYRIAFEGPRGVFDMQVP
                     KNTAYLLEGEATVENLSTEIIKLLVPKLPKNIEAVGVYVYEGYNKGAHLLSKISKS"
     gene            18640..20985
                     /locus_tag="VGA92_08410"
     CDS             18640..20985
                     /locus_tag="VGA92_08410"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9846464.1"
                     /translation="MEPKIKETAWNPQLELQILRHWGDSHLYDFVPQSKNFVIDTPPP
                     YPSGRPWHIGAAAHYSQIDMIARTARMSEHNVYFPIGIDRNGLPVELYTEKKHNIRMQ
                     ETERSKFLELCKSSLDDLEAEMIQIMKSLGISANFANYYRTDSEEYRTLTQSTFIELW
                     KRGIVYKATRPNNYDWVSGTTIADAEITYQDIQTKLVYMKFKIKDSDSEIIIASTRPE
                     LLCACQTVIVNPEDERYTGLVGKKVIIPVINREVSIRTHHSAQKEFGSGAVMVCSYGD
                     QNDVALFREMKLDEVVAIGMSGHMTEAAGKYAGLKVKQARTQIIEDLKNAGLVEKIED
                     ITHRIPVSERSKIPIEIIPMEEYYIRQVDAVEKIRNIGQKIRFYPEMHKQILMNWLDS
                     ISIDWPISRRRFYGTEIPIWYCKTCSEPHVPKPGKYYKPWKDKAPFEKCTQCGSAEFV
                     GEERTFDTWMDSSISPLFISKYNTDDEFFAKTYPATIRPQAKDIVRTWLYYTILRCDQ
                     LTDKAPWSEAWIMGYGLDEKGMKMSKSKGNVIDPIPVIQQFGADAFRFWSASEINHGY
                     DFRCNEQKIEATKKFLSKLWNVSRFISSYPEITYQNLKDISGIPRNIQESDRWILSEL
                     DNLIKECKKGYSEYNFFVPAIAVREFTWNLFAAHYIEMVKARAYGEDFTEGEKNAAIY
                     TLHKVLSTILLLLAPITPFITDFMWQKLYSKETIHKQQLPEPQNLQDMSKFTKEIADF
                     NSKIWNEKKANGLSLKDPISVEIPESLRPFAKDLIPMHKLE"
     gene            complement(20995..21612)
                     /gene="pdxT"
                     /locus_tag="VGA92_08415"
     CDS             complement(20995..21612)
                     /gene="pdxT"
                     /locus_tag="VGA92_08415"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX9846465.1"
                     /translation="MSVNIGVLGMQGDVEENISATRAALGNLGLKGNVRQVKTPDQIE
                     KLDGLIIPGGESTMIGQMSLVNGAIKTIKEKIQSGMPVFGVCAGLILLSKNAKDKVVG
                     KKDQPLLELLDVKVERNAFGRQRDSFEAEISAQPIGIPKSRGVFIRAPSIEDIGKDVQ
                     VVTKFNEKIVAVKQGNILATSFHPELTTDISWHKYFVSLIKPKKK"
     gene            complement(21609..22577)
                     /gene="pdxS"
                     /locus_tag="VGA92_08420"
     CDS             complement(21609..22577)
                     /gene="pdxS"
                     /locus_tag="VGA92_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX9846466.1"
                     /translation="MIPLSGNLGNAKGIVSEKVGTHSITRGTSTLKRGFAHMLKNGVV
                     MDVTTVEQALIAEEAGAVSVMVLDKLPSDVRKAGGVARTASIRVIEEIMDAVTIPVMA
                     KCRIGHVYEAKVLEETNVDMIDESEVLTPADESHHIWKWDFTTPFVNGARSLAEALRR
                     IEEGAAMIRTKGEPGTGNVAEAVTHIKKVNDELRAIKSIYESGDHQDLVKMAREFRVS
                     YELVEQTAKIGRMPVVNFAAGGIATPSDAAYLMSLGCDGIFVGSGIFKAEDAKERARA
                     VVLATTFWEEPDKVKEAQKMIDERQSMLGLDVKTLELRMQERGSTA"
     gene            complement(22648..23178)
                     /locus_tag="VGA92_08425"
     CDS             complement(22648..23178)
                     /locus_tag="VGA92_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846467.1"
                     /translation="MVKLLILIAASIPVILAILIAVPMITNPQIPFSATTSDDKISLE
                     FTKQHLQTTLGVAERIIPQKIEILTISNEGDARYLVTEGGVNQPEKNLKLDKDYVKKL
                     TALIKETGFMHIPIDLIRANDGVTEYDRFSLKVTLNGNTKQIQWAEQNATSTLVPPLI
                     TIIGSELQNFTKKFNN"
     gene            23298..23447
                     /locus_tag="VGA92_08430"
     tRNA            join(23298..23326,23349..23357,23412..23447)
                     /locus_tag="VGA92_08430"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23354..23356,aa:Trp,seq:cca)
     gene            complement(23452..25719)
                     /locus_tag="VGA92_08435"
     CDS             complement(23452..25719)
                     /locus_tag="VGA92_08435"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX9846468.1"
                     /translation="MEIETTPKLVLDVYKKLENNISKFRKAVNRPLTLSEKILVGHLV
                     ELASKEPECAKSYVFLNPDRVALQDVTGQTVMLEFMQAGLKQVALPTTVHCDHLIQAR
                     VEAESDTRAALYENNEVYKFLESASRKYGAGFWKPGAGIIHQVVLENYAFPGGLMIGT
                     DSHTPNAGGLGMIAIGVGGLDAAEVMAGMPWEVLYPRRIGVYLKGSLNGWTAPKDIIL
                     YVAGQLTVSGGTNAVIEYFGPGARTISCTGKATITNMGAEIGATCSIFPYDEKMETYL
                     RATDRSEIADLANKHTELLTEDPQVEQDPTKYFDKVIEIDLSKLEPHIVGPHTPDLAR
                     PISKLAEDVKKNNYIDDISVALIGSCTNSSYEDMSRASSVARQAKQKGVKAKIPLLVT
                     PGSEQIRATIERDGQMQTLKEIGATVLANACGPCIGQWSRPELKRDEPNTIVTSFNRN
                     FPGRNDGKRNTMNFIGSPELVIALSLGGSLSFNPLSDELTAKDGTKFKLQPPEVAPEV
                     PQSGFKMGTDVYVPPASNPETVQVLIDKNSTRLQVLEPFEKWDGLDYVELPIMVKAKG
                     KCTTDHISPAGPWLMYRGHLDKLSDNLLLGAVNAFSGEVGKGKNILNDNIESFPNIAR
                     QYKAKKMRWVIIGDNNYGEGSSREHAALTPRYLGCAAVISKSFARIHETNLKKQGVLA
                     LTFSNPADYDKIQETDRLSLLELSKLEQGRPVKCIITHADGSKEEIQLLYSYNSAQLE
                     WFRKGSALNVLRSKK"
     gene            25917..>26533
                     /locus_tag="VGA92_08440"
     CDS             25917..>26533
                     /locus_tag="VGA92_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846469.1"
                     /translation="MVNKGFPTFGMSQAGAYIAALKNYNLPDFILKKIAKECSSNLLE
                     RGRLDDRLQSMNDYALTMLHRIFIDCESDEGGKFADFRFYAYVASMYHKCDILINETI
                     PGASGKNHKVPVAVKNNGMYIAVAFNKSTGTPVQKREAIKFYNIIDDVKKGDHGTMLT
                     DAILGTSVGFKGESLVELERLSKSRKSDAENRLEIKTANFENRIYS"
BASE COUNT         7698 a         5429 c         5413 g         7993 t
ORIGIN      
        1 tcattttacc taaagaacaa ttattgattt agatgaaaat agaccaaaac gggacatctg
       61 cgaggtgttc gatatgtggg gaataatccc cgaagagaac cgacagataa gatgtccatg
      121 cggtcataca gaagatcgca acatcagcgc tgcacaaaac atcttgtata agggtattca
      181 ggtgcagcta cgagtccttc ggctaaggcc cgacgcgcca caaggtgaag caatgaagca
      241 gttaaaagac gccaagcaga ttgtggtgag ttatctttga acatccagag gtaacagtgc
      301 catatgatcg tgagtaacgt taataccctt atattcaagt aaacaggacg attagcatga
      361 ccacgtatag aaccagtatg cagattgtag cagacctact tacagctact gatcattgtg
      421 gtcaggaagg aatcaaggta acaacccttc taactcaggc aaatctctca cattctaggc
      481 tctccaagtt cattgagaat ctgactggtg caggcctaat caacaaaata gaatttgatg
      541 gaaaaaacac ctttgtcatt acgccaaagg gaaagcagta tcttgaatca tacaaacgat
      601 tccacgacct tgcgggatca tttggtctag acctttagtc ttttcttttc ttcttcgcgc
      661 tttgatggct ctttctgttt ttgaaactag aatatatcac tatgattatt gctccagcaa
      721 gttggccata aaacaactca aggcttggtg ggtctttcat aattgaaaaa atcattccgc
      781 ccacaagcag cgtaaaaacg attactagat acttgtccac acaagacatc aataaacaaa
      841 caatatattt tttcgaaatt ctgagaatgt atgagtaagg tacttgagct gattggcttt
      901 gggctgatcg gaggcacgat ttatgcgtgg attggaatga tcgacaaccc catgcgatgg
      961 ccgatttttt ggggctgtgc cggaggtgcg cttgccatca tactttatcg caaattcaaa
     1021 aaacgcaagt aagttttgta ctagttggtc tacaatgtca ggttagataa ttttcgatcg
     1081 acagatctat aaaggagctc tggatgatac cttcggggag aatgacagaa caaacaagca
     1141 agtggtggga aggacttgct aaagaactgg aagttgacat tgaagcccca gaagaataag
     1201 ttttgttttt aatttaatat tttagagcct cgagcgggat tcgatcccgc gacctaacgc
     1261 ttacgaggcg ttcgctccac caggctgagc tatcgaggca tggttcgctt gttatcagag
     1321 tttattaaaa gcatttgacg ctttgctacc attgaaaaaa tccatttctg gaatacgtgg
     1381 aatttttggt aatgacctta cgctcaaaga tatcctaaaa ttttgcagga attttacgcc
     1441 gctaatcaag tcagacagat gtgttgtagg gcgcgacact aggccatcag gtgagataat
     1501 caaagaaaca gccatggcag cactaatggg ccaaggtatc agcgtctata atcttgggat
     1561 ggtcccaaca ccagtagttt ttagagaggc gcgtaaatat ggcgcagggc tcatcatcac
     1621 gtcatcgcac aacccacttg aatggaatgg tctcaagttc ataatagagg gacgtgggcc
     1681 aaacgaaaaa gagtttgcac agatcctaaa agaaaaaaaa tcaaacaaca aaaaaattgg
     1741 caccgagact gaggtgtcct caaattatgt agatgatgca gcaagaataa ttgggaaaat
     1801 aaatcaaaag ccaaaaattg taatagatgt tggagggggt gcagcagtaa atacagctcc
     1861 ccatcttctg cagagtcttg gctgccaagt tcaaactata aatcaaaatt cgtcaaaaag
     1921 gggtccggat cccacttcag acagtctttc tgagcttgta tcaatgagta agaatgctga
     1981 cattggtttt gcgtttgatt tggatggtga taggcttgta gtagtaaagg atggtaaaaa
     2041 gcagtcacct gacacaacct tggcgcttgg tgtatcaaag gcactggagc ttggatacaa
     2101 aaaattctgt cttagcatag acactagcac atctgttgag aaatacatct tagatcatgg
     2161 tggcagagtc atccgctcaa aggttggcga ggcaaacgta atcgatgtga tgattaggca
     2221 aaaatgtcag gcaggtggtg agggaagtag cggcgggttc atacttccag aattcaacat
     2281 gtgccgcgac ggcatattgt cagcaggctt gattgccgct atgactggaa aaaaaatcat
     2341 tgatcaggtc atagagtttg tgtcaagata tcaccagctt aggacaaaga ttggcatcga
     2401 gtctaatctg catgacaaag tactggacag gtttgagagg aacctaaaag ggaaattcag
     2461 ccagataata acaattgatg gcgtaaaagc cataattgat gatgacactt gggctctggt
     2521 aagaaaatca aacacggaag acattgtgag gatttcagta gagtcaaaca atttacaaaa
     2581 ggcaaaacaa attcaaaaag agataacatc cctagtaagg aaaagccatg accaaactag
     2641 atgaaagaca gataatcaaa attttccaaa gaaacttttc tggtaaaaaa tttgtctcag
     2701 aagatgtaga ggtgtttagg attggcaaaa gctttggagt aatcaagact gacactttgg
     2761 tagaaagcac ggacgtcccc cccggcatga gcccagcaca gattgcgcga aaaagcattg
     2821 ttgcatcggt aagtgatttt gcagcaaagg gagtcagacc actttactgc gttgtttcag
     2881 tctcactgcc aagaaattat tcaaaatcaa aagttttgca gcttgcaagt ggactcaaaa
     2941 atgcatcacg tgaatttggt tttagaattc ttgggggcga tacaaacgaa ggaaaggagc
     3001 tggtaatttc agcaacactg tttggccttg ccaaaaaaat tgcgcgcagg gctggcgcaa
     3061 aagcagggga tcacatcata gttactggtc cctttggaag aacttcggca ggcctcaaga
     3121 ttgttctgca tggaaaaaag gcctcagctg gcttcaaaaa gcttgcaaaa aaagcggttt
     3181 ttttaccaac tccacggcta atctttggag tatcagctgc aacatacatg acgtctgcaa
     3241 tggactctag tgacggtctg tcaactacgc tcaacgagat ggcaaagcag agcaataaga
     3301 gattcgtaat tactcagata ccaaaagatg acaggctgga tagctttgcc aaggataaca
     3361 agcttgatct gattgatctt gttttcaatg gcggagaaga gtatgagata gtagctactg
     3421 catctccaaa agatcttaat aggatcaaaa aaattgcgcg caccaaaaaa atcaggctga
     3481 tttgcattgg gcgtgtgcaa aaaggagccg gtgtattttt gcaaaaaaaa caaaaactcg
     3541 tcaaaattag agaccgagga tggctacatt ttacaaatta gcatgatttc tgcgttgttt
     3601 gacttggcca aatccgataa tgttttttaa accctccaat gtttcggtaa acaatgtcag
     3661 aagaacgtgt agaaaaatgg ggcgttgctc acatttacag cagctacaac aataccatag
     3721 tacacatgac tgacctaaca ggcgcagaaa ctgtggccat aagttcaggc ggtgtgcacg
     3781 ttactgctga cagatacgaa tcctcgccat ttgctgcaat gaaatcatca aatgcagtag
     3841 tcgaggcggc aagaacaaag ggctttactg gatttcacat cagggtaaga gcagttggcg
     3901 gcgttggctc aagagttcca ggacccggag cacaggcagc gattagagcc cttgcaagag
     3961 gcggattcaa gattggtaga atagatgatg taactcctat tcctcacgac accacacgaa
     4021 agaagggtgg aaagcgggga cgaagagtct aaacctcaat taactttatt ttagctttgc
     4081 atccctgaga ttgaaaatag tcggacagaa aattcgtaaa cttttcctca gacttgctgc
     4141 cctttgtctt ttttatcata tcggagaatt ttccattgat ttgtaactgc aggtttgtct
     4201 ttatccataa tttgaaaaac gtccagccaa atctcgagac tggacttcca aactcaaatt
     4261 cgttttgtag tccgcactgc gcagctatgt ctgataacgc gttgttaatc agacgtttgt
     4321 cttccagata atcggttgag cttatctcaa agacccactg aatcattata ttcagactcg
     4381 gttgctctac ttaacttgtc agaaagtgag acaaattttc catcctgctc tacgttgatt
     4441 tttgtttcca tccttacgtt gattccgact gatctgaaca gtgccaaaag ctttcctccg
     4501 tcaatttctt catttatctc cccgctgtga ataagctggc caagtttttc aattacgagt
     4561 ttggtatcgt ttgggtattg ccgctctgcg ttttggagta cctccatgcc cctgtaccct
     4621 aagcgcccaa tcacaatgtc tcgatatgat ggaggttttt gctctgttgg tttttgttgg
     4681 tctttttgcg tctgagaaaa tacgtttttt ttcatttctg ctaggcgttt tgccttgagc
     4741 atctctagtt ctttgtcttc gtcgctcatc cctttatcac accaattgga acaagttttg
     4801 ccaccttggt tgcaatacca agctcgtgtg aaacatttac cactgcatcc acatccttgt
     4861 aggcctctgg tgtctcctct actacgccgt cccttgtcaa cgattttatg aaaattcctt
     4921 tatcatgtag tgattttttg acctgttctt ctgtgtattc gcgccttgcc cttgagcgtg
     4981 acatcattct tcctgcgcca tgtgctgttg agccaaagct taggctcatc gagtttggct
     5041 tgccaagcag aatccagctc ccagttccca tcgagccggg aatcaaaact ggctgtccca
     5101 agtcccggta ttttgttgga atctcttctc ggtttgcagg aaatgcgcgt gtagcaccct
     5161 ttctgtggac taccaccggt ctttcctttc cttcgatttt gtgtttttct actttggcaa
     5221 tgttgtgtgc cacatcataa acaagcttca tttccaagtc agattctgtt tttttaaaga
     5281 cccgctcaaa tgattttctt gtccagtgtg tgatcatctg cctgttgctc caagcaaaat
     5341 tcagtgcagc aaacattgcc tttctgtacg attcaccctc ttcggatgtg tttggaacac
     5401 atgcaagctc cctgtccggc agattaatgt catattttct gagtgcttgc tccgatatcc
     5461 taagataatc gctgcagatc tggtgtccaa atccccttga gccgcaatgt atcagaactg
     5521 taacgtttcc ttctgctatt cccattcttt ttgcggcctc ttcgtcatat attttttcga
     5581 tccgctgaac ctccaaaaaa tgattaccag agccaagact tccaagctgt ggtgctcctc
     5641 tttttcttgc tgtgtttgag accttgtttg ggtctgcatt ttttatttgt ccattttctt
     5701 cgcagacatc tgcatcatca tgcgtcccat acccgtgctc tattgcccag tttacccccc
     5761 tgacaagtac ttcgtcaagc tgagagtaac ttagtttaat tgcgccttca gagccaacgc
     5821 ccgaaggaat tgagttgaaa agatcagtta caagctcttt tagctttggt ctaacatctt
     5881 tttctgaaag attcgtcctt agcaatctaa cgccgcagtt aatgtcgtat cctacgcctc
     5941 cgggggagat cacgccttct tgtgcatcca ttgccgcaac cccgcctact ggaaatccat
     6001 atccttcatg cccatcaggc agaactacgg cgtgtcccag tattccaggt agtgtcgaga
     6061 cgttaactgc ctgccaaatt gttctatctg tcatcatttt tgagagcagg ccctcgtctg
     6121 catatatggt cacagggact ctcattccct tgctggaatc agcgtctatt ctgtactcca
     6181 tgtttccaat tttttttgga gaaatcgatg acatcatcgg taggatgttt tggaagtaat
     6241 ttaaatcata tactaacagg tgtacaaata tcgcacccca aatcagctca actatcagta
     6301 aaaataaaaa ataaagaaaa atacgtttta aattccgtat ttcttctttg caaactctaa
     6361 tgccttttgc gcttcaatca ttttgagttt tgtctttagc tgctctggtt ttagcttgtc
     6421 aggctttgta agttgctttt ttggatcaaa tttgtccgag tatgtttttt ctatgcttag
     6481 tttttggttt gatttcaggc caaaactctt tggagatagt ttgtcgtcag tgtttgccgc
     6541 ttttgcctct gcaggtgaag agttagcaaa gatcaaagca gtagctagaa ttgctgcgag
     6601 tgcagttact ttaattattt tcatcagctc acctcccttt gaaatgttga actctcatcg
     6661 gagtcgattc ttgattttgc aaagacagag agatttgcat cttgtaaatc ttcttgtctt
     6721 agtttttttg ttttcataac cataccatta taaaataata ttagtgaata tccaaccgct
     6781 cgcggtatgc cttattcttg gataaaacca cacacaagac aacttggaaa tattttctgc
     6841 actatctaaa tttagattac tttcgttttg atattttgat attttgagag atcgtagaca
     6901 tattctatct gaatgttggc tgcttgtttt gttttgatct gtgcgtgata atcattgcat
     6961 ttgcctacct gcaaaacctt gcttgataga cccaagtact gctcaaattg ggatttgatt
     7021 aggaatgaag gcttttggat tgtggatttt tctgcgcacg ctcgaaactt gaccagatat
     7081 ttgtctgccc cgatagaaat tgacgataat atttttacgg taattttttt ctcctcggtt
     7141 gtattctgca ttaacgcgtt aagtttttcg tagtgcttgt caattttgct ttttcctaca
     7201 aaataccagt ttggattttt atacaatttt atgcagtcat ttaggaagct tttgttcttg
     7261 tatttttcac gaaatttttg ctcacctagt actttgtatc tctcatacat tgatttacac
     7321 atggtttcat caacttttgc ttccacagaa ttaaagggaa gaaccaaaat ggcaagaatg
     7381 agtactagaa cgcctacaaa ggcgccaaat ttgtttgctt tgtgatttat tattgacatc
     7441 taatccctgt aaaaaaatta gaacacaaac tgacgagtcg tttgggggat gataagtgta
     7501 aagattggga cataccactt tttccattgc ttgctttgac gtcgtagttt gtgttcatga
     7561 ctggttgatt gaagaatatt attagtgcat aatcatagtt ttgcggcatg cctttttaga
     7621 tcaagcattc aatctgtctt gatccaatta tttgtagaca ttctagttca taaaaacagt
     7681 ccaagtgaaa ttgctctaat gttttttgtc cacacacatt acatatcttc tggatatctt
     7741 ttttgcattt ggcacatagt gcacagattg tttgtaaatt tccacatctt ctgcaatgtt
     7801 catctggcat ttcaagatgt ctgacgtatt ttgatataaa gaactagata tgacagtgcg
     7861 gtgtgcttta aattagtaca aatttttgtt tgcctattca actaggacaa aagtaggttt
     7921 ccatctgagc ggatcatttg taattttgta tgcttgctca aaataagtca atgcatcatc
     7981 atatcgcttt gtttcttgta atgcatttgc cttgttgact atggccccaa gcctgccagg
     8041 attgatttcc agtgctttgt cgtatagatc tattgcttca tcatacttgt tttgttcata
     8101 atacgctata cctttgttaa catatgcagc atagagagtg ttttgatcta ccttgtcggc
     8161 atttgctatg accttatcaa aatataccat ggcatcggtt tggttgccat atattgagat
     8221 tgcaattcca gcattaatta tggccctata gtccaaattg ttctttgttt ctcccatttt
     8281 tattgcctga tcaaagatga attttgcttc tgccagtttg ccagttcttg ccgttgcaat
     8341 tcccttgtcc actagagcct tgacgttgtc tggttctcgc tttaatgctt cattgaaata
     8401 aagaatcgca atttcgttat cttcttcttt aatgttttca acaccctttt caagtaattt
     8461 tgtagtgctt gtttctggcg agattggcac aatctcgtct tcttgtgtgt cttcaatcac
     8521 tatgtagcct ttctcaacaa gatactcgat cgcgttgaga aactcccttt ctgtgattga
     8581 gccttcggcc caccatttgg ctatgttttt tacccataat ggaactggtg ctgctgatac
     8641 attctgaaac attagaagaa aaacaaatat caccaaaatg ggaaaaatgt atttcattaa
     8701 aagctaatct cgttcattat ttaaaaaaat attgttagtg aatgggctta agagttgtac
     8761 aactcgagtt gacaataaca gaactcgtgt aaatcaacta attccttttg attacaacac
     8821 gtgcaccttg caaaaactgg atttttgcat tccacgcaga aattagactc gaccattttg
     8881 ccttggcatc ttttgcagag ctgttcatct gtctcactca ccagatcttc cttttgttca
     8941 aggtagattg ttagttggtt catgaatttg ttgtaagaaa atgttattag tgattagccc
     9001 taagtttgcg gcatgccttc tgagatagga gtatacggaa atcttcactc atacaaattg
     9061 tacgtattgc ccacagcaaa gcggcttttt ggaagctatt cctttcgaag gaaagctacc
     9121 ataaagcacc acactaacgt acaaaagatg cttgagatct tggcccttca tggtcctctt
     9181 accacttggg gaatggcaaa ggtggttgtt cacaatgagg tatcaggaat taggacaaag
     9241 gaaaaagaat acaggcggtt gttaaaggga agaaaggaca gaggaaagca ctcacctgga
     9301 gttttggatg ttgggttggt agtagtagat ggtaagaacc acgatcgagc tccctcagat
     9361 ctctacagac tatctttgca tggaatacta tattgtttgg atgtccttga tttaacaaac
     9421 aaagaaattg acatgatggc aaagcattat tcgcctgtcc taccatgggt atttggaaag
     9481 tgggaatatc taaaatcaat tattggatcc gatgtttata gattaaagac actggcaaat
     9541 ggaattttcc tagataacat ccaggtgaca aagatgtcta ggtttccagt ctttgaactt
     9601 ctcacatact tgtcaataaa ataccaagaa tattttgaat acataaatga agaaaagctg
     9661 gcagatcaga tatcctgctg gttttacaca cacttgctta tttcctctgg tacaaaatcc
     9721 tcaatagata acacaaagtg gaagaagatt cttgccgatc aagaaatcaa aaaatggtat
     9781 catggcttta ctggcgaagc gatgcgattc taccaagaac gattcaatac aatcaaaaag
     9841 cttggaaaaa gataatgcta cgcgtatttt tcgattagcc cttccaatat ttcaaaggaa
     9901 taaggctttg atatagaatc aagcaacgac atgcttttgg cttttaggta ttttttttca
     9961 tcttggttgt atgcggtaat aagaacaact ttggcgttgg gatcaaactg ctttatttta
    10021 aaaaacgcgt catagccatc catcttgggc atccttatgt ccataaatgt aagatttggc
    10081 ctcacatcct tgtattttga tataccttct tctccatcgc cggcagtaaa tacatcgtgc
    10141 cccataccct ttatcataaa agcagtattt tccagcagat caacgtcgtc atccactaac
    10201 aagatagtct ttttgttgct cacgttaatt tagagagccg tgacttgata aaagatttac
    10261 taaagaatct ttcattttgt tttagcttct aaaggaatag ttattgtgaa aatagttgga
    10321 ttgttttgaa atttgatcga gcccccatgc tgctctataa tgtttttgca gcttgcaagc
    10381 ccaaggccgg ttcctttttg ttttgttgtt acaagaggtt tgaaaacatc ggaaatgact
    10441 ttttgtggta cgcccgcacc actatcctct atttctatta ctgcgttttc atttactttt
    10501 ttgattttta tcatgattct tccctgagta tcaccaattg cctgaacaga atttagtatc
    10561 aaattaatga atactatttc catctttttt tcatcgcagg taactacaac atcatctccc
    10621 tgaataataa cttggattgt ttttgggatt tcaattgatt tgatggcaga gtctattatt
    10681 gcatttattg aaaccggttt tagggaaagc ggcgtaactc tcacaaaatt tagaacgtct
    10741 tctacttgat gtgccattcg agaaactgcc ctgttcatcc tctgaataac ttcttcagtg
    10801 ttagaatcag ttgaagtgtt tctctttatg atatccatac cgcttcgaat tgtccccagt
    10861 gggtttttca tatcatgtgc catgcttgct gcaagctcac caatggcagt cagtctttcg
    10921 ttttttatgc gtgcgtttgc ttcggcaagt cttttttctg tttcaaataa ttttttcaaa
    10981 gaaacctcca tttccccaaa agagtctatt atagagttga tttcggtgat cttgctcttt
    11041 tgtattttgg catcaaagtt tccctttccc aaaagtgcag taagttttga tagttttgca
    11101 agaggagttg tgacaaaata tgataatatc actcccagca ttatagcaga tattattccg
    11161 actagtgttg tgaacaaata gtttttttca agttcttcaa attcgtccac tactgaagat
    11221 tcttgctcta ttacaacaat ccagtcaaag ccactaaagt ctctaaaccc aatagacttt
    11281 gcataagaga caaaaactga atttgatgga ccatactcaa agattccatt ttctgtgacc
    11341 aatctgggaa aatattcctt aggcgcatcc gttgggaaaa actttccatt ttcatagatc
    11401 acatttccgt tttggtcaag cagtatgaca gatttttttg atagatgtaa taggtcggca
    11461 tcgtttagaa agtctgtaag taaaagacgg agactgacag agattctcaa tgtgccaaca
    11521 tagtctgaag aatcatctag aatcggatat gcaaaggcaa ttacataatc gttgtaattt
    11581 tcgtcaaatt ctattgggcc aatatagttt tgcttgtttt ttgtaatttg ccaccattcc
    11641 tcatcagatt gcaagtaatc tgacttaccg cttgccagag caatattagc accatattgg
    11701 tttgttacaa ataactcctt taccacatca taatcatact cttgcccata ttgcgaaata
    11761 aaatcagcaa gctcatctga aatttcgttc tctaatatac cgcgtaaaaa tggtgttttt
    11821 ttattgcttt cagatacttc aattagtgat tcctgttctt taagattaga cgagtcaaat
    11881 ttttgattgg attcaattac tgcagattgg atttgttcta gttttgttag gctttgaaaa
    11941 acagcggttt tttcataaat ggttttgtca agatccgtcg ctatgctgtt tgcttgtgct
    12001 tggattctca gcaaggctat gttcttagct tctttgagcg tgaaatcgta tgttaaaaag
    12061 ctgtgaatcg catataccaa aaacaatatc gtgattgaca ttatcagcaa atgtgaaatt
    12121 ttcaattgtg tctgatgatg tcttttttgc atttaaactc tagttttttg gtgcatgaaa
    12181 aaggcatgcc gcaaactgtt agttatcgac taataatatt tagtcaaaaa tacttgcatg
    12241 aatcaagtag gaataatcaa caatactgct tccaatattg aatcgtatgc ggtcaacttg
    12301 gaaaaagata gacttgcaac ttttgatagt ttgattaaaa aattatttta tgcgtgtgaa
    12361 tattgtgatg gtgcactcat acagctggcc atttgtattg tttgtagaag agcaatcatt
    12421 agaaaatgca ctcagtgtag cactgaatta aaaatctcac accccatgtg tgaaattcca
    12481 ggcagaatcg ataatcccag taacgattct gctttggtgg tgaaatatta atgataaaac
    12541 taattggcat ctccattctt gcagtattgc ttgtagtagc tattgcttct ctgcacccaa
    12601 ccgatgcaat tacctatgag aagcaagaag actccaaata cgtcaagcag aaaatagttg
    12661 aaccactaaa aggcaagaat ggttggtgga agtacctgct taaagtgtgc gcagatgacc
    12721 gtaacttggg aattacagaa gttgtattga gttctgatat ggaaacgatt tatcagggcg
    12781 taaacaaagg ccttgccaaa ggtaaatgca cctattttgg tgcagtaatg aaggcaaaag
    12841 atggcaacac cttaggatac aagattacac agactcatga agcagtagag aagatagttg
    12901 aatcaaagca agtaaagcta agttcgagta gctggtctga aataatgcga tacaagttca
    12961 tcctaggctt ctactagaga ctcagaaggc atgccgcaaa tatgcaaaaa ggacttaata
    13021 attaattgaa aaaatgctat caatgcgtgt cctgtattca gaaaagcacc gtcattatag
    13081 gtggagttgg gcaaatcctc ccaactccac ctatacgtat gtgcaaaaaa ggagtttgtg
    13141 atgattatga ggtgtaaaaa ctgtggaaag gagtttgaga gcaggcgatc agagttttgt
    13201 tccgttgaat gtgcaggtca gtatgcagaa tagattattg ccatatcgga gaaataccaa
    13261 aaatgcaaat taaatttttt aaaatgtttt tgaaaagtgg gattgtttta tacacatcgt
    13321 tactacgtcc cagatatttg atttcaagta acaacataac caaacaagag ctaaacaata
    13381 cttaagtaga ataattttcg aggagatttg taaaaaacga acgattcatg aaactcacaa
    13441 tatcactgct tttggttgtt atagtaacat tgttttcagc caatgctctt gcacagccct
    13501 acattttgct tgacaagcca acctacagct atggcaacac aatcaaagtg tcagggagtg
    13561 tagaatatca ggagggaatg ttcattataa tccagattag aagcccatct gacatagtag
    13621 caatagatca aatatttccc tctcgttctg gaaaattttc agctgtcttt gatacggagg
    13681 gcccaaaatg gggagaaact gggacatata ctgttttagt atcataccaa ggacaaaaga
    13741 cagaaagaac attccagttc tcaaagcccc agccaagtcc aactgaaacg cccgcagtac
    13801 aagaaccccc aacgccccca aaaccaaaga ttacaatcaa gggatttcca gacccaacaa
    13861 aatcgcctca gtattactac gaacgataca acgatgatgt agagtttaag cggtggtttg
    13921 attctacatt tgtaggatat acaatacaag atgtcgttgg atacaagcca acacgtgttg
    13981 ccggatttcc agacccaaat cagccaccac aatactacat tgacaggtac aataacgaaa
    14041 aacccttcat ggattggttt gatcagtact tctcagaaat gacaatctac gatgtagtgg
    14101 gcgttacaga gcaaataaga acaattgcgc caagttgggt aaaacagtac gcggggatgt
    14161 ggtcaaatga agaaatcgac gatgagcgct ttattgaggg gatcgctgat ctgataagac
    14221 agaacataat tgaggttaat gccgatataa caaaacagga caatcctgat aagacgattc
    14281 cagtttggtt caaaagaaac gcaagttggt acagccagga tctaatatca gaagacgcct
    14341 ttttatcagg aattcagtat ctaattgaga aagagatcat agtggtgtga gcttacttgc
    14401 ctagctcaaa aacattggcg taagtagatt tatttttgaa tcaattttag aaactttgtg
    14461 cctattttac caattgacgg tggccgttat ggtacaaaag agatgcttgc aattttcaac
    14521 gatcagaaaa agatagacta tcagctccaa atcgaaggtg ctgctgcagt agctcaagga
    14581 cagattggca tcatcccaaa atccgctgca cagaacattt ttgccgtctc aaagtcaggt
    14641 aaaatcactg taaagagaat caagcatctt gaggcaaaaa gtgatcatga taccgcggca
    14701 ctagtcgaag cattaagcga aaagtgcaaa ccccaagcta ggccctgggt tcattatggc
    14761 ctgaccagta atgatttagt cgatacgagc acttcaatgc aaatgaaaga ttcacttgta
    14821 ataattgagc ccaaggttgc acgattggcg ttacttttga caaaaaaggc aatggagtac
    14881 aaggacgttc ctgcagtagg tagaacgcat ggccagcacg caagcataat ttcgtttggt
    14941 ctaaaatttg caaactgggc ttctgagatg gcaaaacaca ttgagcgaat cgaggaaaag
    15001 aaaaaacgaa tcctaatttg caaaacactt ggcgtagttg gtactggttc tcttatgggt
    15061 gtaaaggcat tagaagtcca aaaaagggtg gcaaaaaaac ttggattgtt tccagccgag
    15121 gtcacaactc aaatcatacc aagggaaaga catgctgaat acatttttct tcttgcactg
    15181 attggaagta cacttgaaaa aatatcaatt gagatccgca acttgcagcg aacagaaatt
    15241 ggtgagatgt ctgagcactt taaggaaggc cagatgggca gtagtgcagt tccagtaaag
    15301 agaaatccaa tcaaaagcga gcgaatctcg tcattatcca agcttttgcg cagccagatg
    15361 agtgcctcat ttgaaaacat tccgttgtgg catgagcgcg acctctcaaa ctctgcaaat
    15421 gagaggttca ccataccaat gtgctctatt ttgcttgacg agatgattga aaccatgatt
    15481 cgagtaattg aaaagctaaa ggtagatgta gaaaaaatag agcaaaatct ctatatcaca
    15541 aaggggcaga tatttgccga gtttgtgctc gaagctttga tcaaaaaagg cgttccaaga
    15601 tttgaagcgt acagagatgt tcagcgagtt gcatttgagg cacatgccag caaagaggac
    15661 tatatcgacg cagtaaaagg agacaaggta atgtcttcta aactaacaga ggatgaaatt
    15721 gataagatat tttctccatc aagtcacctc ggtgcatctt cgcaaataat cacaaacgtg
    15781 aacagaacag tccaaaaggt ttgtaagcgt tttctctaac tagatcacgt tataaacacc
    15841 atcacaccta ttatttttag gctaaactcg aatatgaaag ataaagacaa attagatgaa
    15901 ttgcagtatt tttcacaaga atctttaaag aaagtttggt ttaatgctaa agacgatgtc
    15961 tggaatagat atttaaaata aattcactat tgattaatca atgactcggt ttgttaagta
    16021 atttatcatc aaatcatggt ttatttttta gtaaagtctt gtgaattaaa gaaccatact
    16081 gtaatgcctt tttgcgcttg cttgccgaga tttctggcac gcctatctgc aaggctagct
    16141 ttcgtatgat gtgttttctt tgcagatcat tttcgtcttt gatcttatca gagattggca
    16201 cagattttgc gtaatttatg aaatcatcag ataggagagg ctggactata ttgacaccaa
    16261 attcagaaca aacctcgttt accgctctca tcatatttag ctcattttct aactttgaat
    16321 tcattagttc cattaccact tgttctcctc cttttattgc atctcgatac gcattatatc
    16381 cacaaaacag ctcatcaatt ccgtttgcgg taactaccac atttagacca aggctttttg
    16441 caagcttggc tacatgataa aatgctatac tgttttcatt ccatgagaga ttatcggttt
    16501 ttattttaga tttgatgcta gttgagattt tatcaaactc gttaggattt atttccaaaa
    16561 ttttgtgtgg taatctcaaa atctcgttga cctgttttga aaactcaatg tcatgcgagc
    16621 ccgcaaaccc gattgtcagt agcgtaacgt cataattcat gtcactgcaa atttttgcaa
    16681 taactgagct gtcaacgccc ccagaaaacg caaccccaat tttttttcca gttatcgttt
    16741 gagagatggc attgctgact ttttctaaga gcgtatcaat ttggcgcatg atttgttgcc
    16801 atatctccta tacaaagaag ttactattgc aaaattaaag aataaatcat gtagccaact
    16861 atccaggatt catttgagac acttgactgg atgatgtgtt tagtcgagtt tacaggtcaa
    16921 ataaggcata ccgcaaagcg gcatacattc tcatatattt ctgagcgcaa cacatgtcat
    16981 gtcaacgatt caaatcgaaa ggcaatggca acctgagcca aaaaaaaatc tggtcaaaag
    17041 tttcccagta aaagaaggtg caaagattta cgggcagatc tccgcagcca aatgcgtaat
    17101 ctgttcaacg ccaaaagtgc cctatgtctt tggtcaagga aaaaccccac ttgtggtaat
    17161 ttgtccaaag tgtggacaca aatcatgggc tgactgagtt gttggcacat gattttggca
    17221 ttttatcgac aacggagtga ttggtcatga agaagatctg tgtgaattgt ggaaaagtat
    17281 acgaaaacag ggcaagctat ttttgttcac aagactgttt tgaggaaata attaacagtt
    17341 gaattcaaac tcaagaaaac aactagattc caagttttgt tattattctt gtgttagcat
    17401 gaaagaacaa gtgatttaat tttgcgatca ggttcaaaat tgccaaaatt attaattgtg
    17461 tctgatttat caacaagcca tgggacctgc aaaactatcc gatgaacaga tcaaatcaga
    17521 acttgcaaat ttgagtggct ggtcgttaaa ggacggcaag cttcacaagg attttgtttt
    17581 tggcgacttt attgaggcct ttggctttat gacaaaagca tcactgcaca ttgagaagat
    17641 gaaccaccat ccagaatggt tcaacgtgta caacaagatt agcgtggact tgatgaccca
    17701 tgacgctgga ggtatcacac aaaacgacat caacttggca aaaacactca attcgttgca
    17761 gtagctaatt cgtagtcaat ttatatacag aatgaagtcc aaaagctcca aatgactaca
    17821 aatcctgcta ttttggatgc cgactttagg tacattgaca aaaaaggcac gctgctaaag
    17881 tcacgaaccg aactttcggt atcacagatg ctttcgtttt taggaatcga ttatgattac
    17941 caacacaaag taatcttgcc aggcggaaaa gaattcttga ttgattttaa gactgatgac
    18001 ggcaagctaa tcgaggtaat tgattctgac caagacatcg acaagtacaa gcaagtaaaa
    18061 caatttctcc cagacgcaaa agtcatggca gtaggacatc ccaagtttgc agcaaaaatc
    18121 aaagagcttg acgatgtcgt gttttatgat accaaggacg cacagtcggg ttctattttc
    18181 atagaagacc catcgtttgc attcgattat gcgcacattt tgccactagt tgaaaaatgc
    18241 tccatactgc atggacacac atcgtctgta ctagtagaat tagtaggtga tatgaaaaac
    18301 aatctactaa ttgattttgg cgaggcaaaa aagataatca aggaaataat ctctattttg
    18361 gatcacaagt ttttcataaa cagaaaatat ctaaaaagcg aggatccctc ccactatagg
    18421 attgcgtttg agggaccaag aggggtcttt gatatgcagg ttccaaaaaa taccgcttac
    18481 ctgcttgaag gggaggcaac ggtggagaat ctttcaactg agataataaa gctcctcgta
    18541 ccaaagcttc caaaaaacat cgaggcagta ggagtttatg tctacgaagg ctacaataag
    18601 ggcgcacacc ttttatccaa aatctccaag tcctagggca tggaaccaaa gatcaaagag
    18661 actgcatgga atccacagct ggaactgcag attctcaggc attggggaga ctcgcactta
    18721 tacgattttg ttccgcagag caaaaacttt gtaattgaca cgcctccgcc atacccatct
    18781 ggaaggcctt ggcacattgg cgctgctgcc cattattccc aaattgacat gattgctagg
    18841 actgcaagaa tgtctgagca caatgtctat tttccaatag ggattgacag aaacggcctc
    18901 ccagttgagc tgtacactga aaaaaagcac aacatccgca tgcaggaaac tgagcgctcc
    18961 aagttcctag agttgtgcaa aagctccctt gacgatttgg aggcagaaat gatccaaatc
    19021 atgaaaagcc ttggcatttc tgcaaacttt gcaaattact accgaacaga ctctgaagag
    19081 tacagaactc tcacacaatc aacatttatc gagttatgga agcgcggaat tgtctacaag
    19141 gcaacgcgtc caaacaacta cgactgggtt tctggaacga caattgccga tgccgaaatt
    19201 acataccaag acatccaaac aaagctcgtc tacatgaaat ttaagatcaa agattccgac
    19261 agcgagataa taattgcaag tacaagacct gagctgttgt gcgcatgcca gacagtaatt
    19321 gtaaatcctg aagatgagag atacacaggg cttgttggta aaaaggtaat cattccagta
    19381 atcaatagag aagtctcaat cagaacacac cactctgctc aaaaagagtt tggatccggc
    19441 gcagtgatgg tgtgtagcta tggtgatcaa aacgatgttg cattatttag agaaatgaag
    19501 cttgacgaag ttgtcgcaat cggaatgagc ggccatatga cagaagctgc cggaaaatac
    19561 gctggcctca aagtaaaaca ggcacgaacc cagataattg aggatttgaa aaatgcgggc
    19621 ctggttgaga aaatagaaga catcacacac cgcatccctg tttctgagcg aagtaaaatt
    19681 ccaattgaga tcattccaat ggaggaatat tacataagac aagtagatgc tgttgaaaaa
    19741 ataagaaaca ttggacaaaa aatccgcttc tatccagaga tgcacaagca gatcctaatg
    19801 aactggcttg attcgatttc aatcgactgg ccaatttcac gcagaagatt ctatggaact
    19861 gaaatcccaa tatggtattg caaaacctgc tcagagccgc acgtgccaaa gccgggaaaa
    19921 tactacaagc cgtggaaaga caaagcaccg tttgagaaat gtacccagtg cggctcagct
    19981 gagtttgttg gagaggagag aacctttgat acttggatgg actccagcat ctcgccatta
    20041 tttatttcaa aatacaacac cgatgacgag ttttttgcaa aaacatatcc tgcaaccata
    20101 cgaccgcagg caaaagacat tgtcagaact tggctctact acaccatact aagatgtgat
    20161 cagctgacag acaaggcacc gtggtctgag gcctggataa tgggatacgg tcttgacgaa
    20221 aagggaatga agatgagcaa aagcaaggga aacgtaattg accccattcc cgtcatccag
    20281 cagtttggtg cagacgcatt taggttctgg agtgcaagtg agataaacca tggatatgat
    20341 tttaggtgca atgagcaaaa aattgaggca accaaaaaat tcctctccaa gctctggaac
    20401 gtatcaagat tcatctcaag ctatcctgag ataacgtatc aaaatctaaa agacatctct
    20461 ggaataccaa ggaacatcca agaatccgac aggtggatac tatctgagct tgacaacctg
    20521 atcaaggaat gcaaaaaagg ctacagtgaa tacaactttt ttgttccagc cattgctgtg
    20581 cgagagttta cgtggaatct ttttgctgcg cattatatcg agatggtaaa ggcacgtgcc
    20641 tacggtgagg actttactga gggtgaaaaa aatgccgcaa tctacacttt gcacaaggtg
    20701 ctttctacaa ttttgttgct tttggcaccg attacgccat ttatcacgga ttttatgtgg
    20761 caaaagctgt actctaaaga aacaatccac aagcagcagc taccagagcc gcaaaatctc
    20821 caagacatga gcaagtttac taaagagatt gcagacttta actccaaaat ttggaatgag
    20881 aagaaggcaa atggcttgtc gctaaaggac cctatttcag tagagattcc agaatctctc
    20941 aggccgtttg caaaggatct aattccaatg cacaagctag agtaaaatta aaaattactt
    21001 tttctttggc tttatcagac tgacaaaata tttgtgccat gaaatatcag tcgtaagttc
    21061 tgggtgaaac gatgttgcca agatatttcc ctgttttact gcgacaattt tttcattaaa
    21121 ttttgtgaca acttggacgt cctttcctat atcttcaatc gatggtgctc gaataaagac
    21181 gcctctgctc tttggaatgc caattggctg agcagaaatt tccgcctcaa acgagtcccg
    21241 ctggcgccca aaggcgtttc tttccacttt gacatcgagt agctccaaaa gcggttggtc
    21301 ttttttgcca acaactttgt ccttggcgtt ttttgaaagc agaataagcc ctgcacagac
    21361 cccaaagacg ggcattcctg actggatttt ttctttgatt gtctttattg cgccatttac
    21421 cagcgacatc tgtccaatca tcgtactctc gccgcctgga attatgaggc catcaagttt
    21481 ttctatctgg tctggtgtct ttacctgcct tacgtttcct tttagtccca gattgccaag
    21541 tgctgcccta gtagctgaaa tgttttcttc cacatcgccc tgcatcccaa ggacgccaat
    21601 gtttacactc atgccgtgct tccacgctct tgcattcgca gctctaatgt ctttacgtca
    21661 aggccaagca ttgactgtct ttcatcaatc attttctgag cttcttttac cttgtctggc
    21721 tcctcccaga atgttgttgc aagcactact gcacgcgctc gctctttggc atcctctgct
    21781 ttgaatatgc cagagcctac aaaaatcccg tcgcatccaa gtgacatgag ataagcggca
    21841 tcagatggtg ttgcaattcc tcctgctgca aaattaacta ctggcattct tccaatcttt
    21901 gctgtctgct ctacaagctc gtaagagact cggaactctc ttgccatttt tactaggtct
    21961 tggtgatcac ctgactcgta gattgatttg attgccctaa gctcgtcatt tacctttttt
    22021 atgtgtgtga cagcttcggc aacgtttcca gttcctggct cgccctttgt tctaatcatc
    22081 gcggcgcctt cctctattct tcttagtgcc tcagccaaag aacgcgcgcc gttaacaaat
    22141 ggcgttgtaa aatcccactt ccaaatatga tgtgattcgt ccgctggtgt caaaacctcg
    22201 gactcgtcaa tcatgtctac gtttgtctcc tctagtacct tggcctcata gacgtgtcct
    22261 attctacact ttgccataac aggaattgtt actgcatcca taatttcttc aataactcta
    22321 atgcttgcag tccttgcaac gccgccagct tttcttacat ctgatgggag cttgtctaaa
    22381 accattacag ataccgcacc cgcctcttcc gcaattagtg cttgctcaac tgttgtaaca
    22441 tccattacta caccattttt gagcatgtgc gcaaatccgc gctttagtgt agatgttccc
    22501 ctagtgatgg aatgagtgcc aaccttttcg ctaactatgc cctttgcgtt gccaagattg
    22561 ccagaaagag gaatcaataa ctctaattgt caatttgcat atttataata gtcgactaga
    22621 tctgcgtacg ggatttagtt ttggatgtca gttgttgaat ttttttgtaa agttttgcag
    22681 ctcagatcct attattgtta tgagcggcgg gaccagagtg gatgtggcgt tttgctcagc
    22741 ccactgaatt tgttttgtat ttccatttag tgtaaccttg agtgagaatc tgtcgtattc
    22801 tgtcacacca tcgtttgctc gaatcagatc aattggaatg tgcataaatc cagtctcctt
    22861 gattagcgct gtaagctttt ttacgtaatc tttgtcaagc tttagatttt tttctggctg
    22921 gtttactccg ccttccgtaa ctagatacct tgcatcgccc tcatttgaga tggtcagtat
    22981 ctctattttc tgcggtatga ttctctcggc tactcccaat gtggtttgca gatgctgctt
    23041 tgtaaactct aatgagattt tgtcatctga ggtcgttgcg ctaaatggga tctgagggtt
    23101 tgtgatcata ggcactgcaa tcaggattgc caaaattacc ggaattgatg ctgcaatcag
    23161 tatgagcagt tttaccattc aaagtattga ttttgtgatt cgataataaa tctaattttt
    23221 gatgacaaat tcttaggata atctgcacaa aacactacca gttagaataa atcccagtcc
    23281 taatttttca attctgtggg gtcgtagcct agcctggtag ggcgactatg atgtcaagtc
    23341 atcgctaaag gctccagaag tgtacaaccc aaactgattt cacaagatga tgtgagtttg
    23401 gagctgcagt gacctgtaga tcgccggttc aactccggtc ggccccacgt cttatttctt
    23461 tgaacgcagg acgttgagcg cagagccttt cctaaaccat tccagttgtg cagagttgta
    23521 agaatacaat aactgaatct cttcttttga gccatctgcg tgtgtgatta tgcattttac
    23581 tggtcgtccc tgctccagct tgctcagctc taaaagtgag agtctgtctg tttcctggat
    23641 tttgtcgtaa tctgcaggat ttgaaaatgt gagtgccaaa accccctgct tttttagatt
    23701 tgtctcatgg attcttgcaa agctttttga aattactgct gcgcacccaa ggtaccttgg
    23761 ggtgagggct gcatgctccc tgctgcttcc ctcgccatag ttattatcgc caattattac
    23821 ccacctcatt ttctttgcct tgtactgtcg cgcaatgttt ggaaatgatt ctatattgtc
    23881 atttaggata tttttgccct ttcccacctc gccagaaaat gcatttactg cccccaaaag
    23941 caaattgtcg cttagcttgt ccaggtgtcc cctatacata agccacgggc ctgcaggcga
    24001 gatgtgatca gttgtacact tgccttttgc cttgaccatg attggcagct caacatagtc
    24061 taatccgtcc catttttcaa acggctccaa tacttgcagc ctagtgctgt ttttgtcaat
    24121 taacacttgg actgtttctg ggttgcttgc cggtgggaca tagacatctg ttcccatttt
    24181 aaacccgctt tgcggcacct ctggggccac ctccgggggc tggagcttga attttgttcc
    24241 gtcctttgcg gttagctcat cacttagggg attaaacgaa aggcttccgc caagcgatag
    24301 tgcaattaca agctcggggc ttccaataaa gttcattgtg tttcttttgc cgtcgtttct
    24361 tccaggaaag tttcgattaa acgacgtcac aattgtgttt ggctcgtctc tttttagctc
    24421 aggcctgctc cactgcccaa tgcatgggcc gcatgcatta gcaagaactg ttgcgccaat
    24481 ctcctttagt gtctgcatct gtccgtcacg ctcaattgtt gccctaatct gctcagagcc
    24541 cggtgttaca agtagtggaa tctttgcctt tactcctttt tgttttgcct gtcttgcgac
    24601 gcttgatgcc ctagacatgt cctcatagga agagtttgtg cagcttccaa ttagtgcaac
    24661 tgatatgtca tcaatgtagt tgtttttctt tacatcctct gctagctttg agattggcct
    24721 tgccaagtct ggggtgtggg gacctacgat atgtggctct agttttgaga ggtcgatttc
    24781 aattacctta tcaaaatatt tggttgggtc ttgctctact tgcggatcct cagttaaaag
    24841 ttcagtgtgc ttgtttgcca ggtctgctat ttcgctgcgg tcagttgccc taaggtatgt
    24901 ctccattttt tcatcgtatg gaaatattga acatgttgcg ccaatttctg cacccatgtt
    24961 tgtaatcgtt gccttgcctg tgcagctgat tgtccgcgct cccggcccaa agtattctat
    25021 taccgcattt gtcccgccag atacggtaag ctggcctgcc acatacagta tgatgtcttt
    25081 tggtgccgtc cagccgttaa gtgatccttt tagataaaca ccgattcgtc taggatagag
    25141 gacctcccat ggcatgcctg ccatcacctc tgctgcatca agaccaccaa cacctattgc
    25201 aatcattcca aggccgcctg cgtttggcgt gtgcgagtcg gttccaatca tgaggcctcc
    25261 aggaaacgca tagttttcta aaactacttg gtgtattatt ccagcaccgg gcttccaaaa
    25321 tcccgcacca tattttcgcg aagctgattc taagaacttg tagacttcgt tgttttcgta
    25381 gagtgctgct ctggtatctg actctgcctc aacacgcgcc tgaatcaaat ggtcgcagtg
    25441 aaccgtagtt ggcagagcaa cctgcttgag tcctgcttgc ataaattcca gcattactgt
    25501 ttggcctgta acgtcctgta gtgccactct atccggattc aaaaagacgt agctctttgc
    25561 acactctggc tcttttgagg ccagctcaac aagatgaccg acaagaatct tttcgcttag
    25621 tgtaagaggt ctgttgactg cttttctaaa tttggaaatg ttattttcta attttttata
    25681 aacgtcaagc acaagttttg gggttgtttc tatttccatt aattatcaca gcaagtaatt
    25741 atctgagtta tgagttataa atcgttatag agttgcgcaa ttttgtttta gtttcagatc
    25801 atgctccagc ggcatctgtt ttttgtgatt ttgctaacat taataagcca aaaacgacgc
    25861 ataccacacg gggtttcagg ttttgataca tctgtttgct aaagggggcc atctgcttgg
    25921 tcaataaggg ctttccaact tttggaatgt cgcaagcagg cgcgtacatt gccgctctga
    25981 aaaactataa tcttcctgat tttattttaa aaaagattgc aaaagaatgc agttctaatc
    26041 ttttagaaag gggccgtctt gatgacagac ttcaaagcat gaatgactat gccctgacaa
    26101 tgctccaccg aatttttatt gactgtgaga gtgacgaggg tggcaagttt gccgattttc
    26161 gattttacgc ttatgtcgca agcatgtacc ataaatgcga cattttgatc aacgaaacaa
    26221 ttcctggagc atcaggaaag aaccacaagg ttccagtagc agtcaaaaac aacggaatgt
    26281 acattgcagt tgcctttaac aagtcaactg gaactcccgt tcagaaaagg gaggcaatca
    26341 agttctacaa cattatagat gacgtcaaaa aaggcgacca tggtacgatg ttaacggacg
    26401 caatccttgg aacatctgtt ggattcaaag gcgagtcatt agttgaacta gaaaggctca
    26461 gcaagtcaag aaaatccgat gcagaaaaca gattagagat aaagactgca aactttgaaa
    26521 acagaattta ctc
//
LOCUS       DASWKH010000111         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2886672, whole genome shotgun sequence.
ACCESSION   DASWKH010000111 DASWKH010000000
VERSION     DASWKH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2886672"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1056)
                     /locus_tag="VGA92_08445"
     CDS             complement(10..1056)
                     /locus_tag="VGA92_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX9846470.1"
                     /translation="MVTAALLVYAGVKSYSLKGKLLTRKDFQTLAEARDLDELVTRIK
                     NTAYNETISKVQKPYTSQKIELALRDRQAEMHYKMIKSVGGSTLLFAYYLKFILRNLK
                     LIIKGKILDRPQEEIEGMLSLHAEELINERDIILKALVAKDVAESINTLRGIGIGSEV
                     EKAYALYTEKKQIQILDTYFDKFFYEKLSHAITNSSDFSVHPLCGREVDFYNILSILR
                     GKFWGLDENQIQNLIVPHSASSSKELLTRMISADSIKVALNELVSTPYKDIIPQQENP
                     IDAISQLENSFERLIHDAMQREFVRVFSFSTVVAITRLIEYEVRNLSSIAFAVEQKIP
                     VDVMMSKMILKEGE"
     gene            1224..1931
                     /locus_tag="VGA92_08450"
     CDS             1224..1931
                     /locus_tag="VGA92_08450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX9846471.1"
                     /translation="MPISELYDINPKSWMKKFQKNSILYLVKMAVFYHLLSIGLTYVG
                     SQLVVRVITDYQEPSFPVSIVMALTSGPIEEVLFFGLPYYLTGNPYAVLVLGSIWSAA
                     HIFNTQVFQLNSLGYVTFLATIPHLFFSLRTWISGKGWFAILFHTGWNLAFLLSYCFA
                     GIRNCSVFGEGDYMALDLFALGLAGSLLSILYLLYSKNKISKRKFRFTMTSSVAAFVV
                     FEILVNLKYVELFFFNS"
     gene            complement(1921..2544)
                     /locus_tag="VGA92_08455"
     CDS             complement(1921..2544)
                     /locus_tag="VGA92_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX9846472.1"
                     /translation="MSSHEQTQISLFRTILEEGPLTLYVANAKSSSPIGTIHRHFREM
                     MQTKKIKVYTNLEDKSRGKIHYGPTLLGMIHFYRLDKILQARLENYFLKWIRFDNFLS
                     ELKDEGFDLNDIARSRDVKNLFKKYVHYFAGVEDQIAALREPNSIPRDILLYIGEFLL
                     VRRPEYMKIWEELYGKMPIIRKNVDGYMQSTIEFYEKLKKKNKAIKS"
     gene            2607..>3783
                     /locus_tag="VGA92_08460"
     CDS             2607..>3783
                     /locus_tag="VGA92_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846473.1"
                     /translation="MQLKTFAVLLFAMLIFTNIPAFAAQLSWDARSFDSAVAPKFTFQ
                     RTVLIEYEDGGIIADDLRGKQTLVQFSADRSTKGVEGLVQKINENLQQSGSTVWVSDL
                     TLQYSAELIGRESSTTIDYKITMIPTISGFLIREYNEDVSALFDVNWRGIKVDDPIVL
                     SDSEQEIEINRPISFLKKEFPTVYDVITETGSEKILEFSMIDASGIKQLPIAKWHSLT
                     DPTAIISDTSQYGFKGEVLTAFSMGESTIFTPTKEKFSEIVFVSDKEYKIQTHEAADN
                     ANLFVPGYAVPTMIVEHEAIGASPTALSGTPPSQQGQFPIFVIYGMAGMAAAGAGGFF
                     WWSSKKAKKDLKLGQSGIDPTLLRGVETSSASGGYKTNRGEAHLVGDTMYQQTKSVYD
                     "
BASE COUNT         1065 a          719 c          778 g         1221 t
ORIGIN      
        1 ttgcaagtgc tactcgcctt cttttagaat catctttgac atcatgacat caactggaat
       61 tttctgctct actgcaaatg caattgagga gaggtttctt acctcgtact caattagtct
      121 agttatcgca acaacagttg agaagctgaa aactcgtaca aactcgcgct gcatcgcatc
      181 gtggattagc cgctcaaagg aattttcgag ctggcttatt gcgtcaattg gattttcttg
      241 ctgtggtatg atgtccttgt agggtgtgct gaccagctca tttaaggcga ctttgataga
      301 atcagcagat atcatccttg ttaggagttc ttttgaactg ctagctgaat gcggaacgat
      361 tagattttgg atttggttct catcgaggcc ccagaacttg ccacgtaaaa tgctcaaaat
      421 attataaaag tcgacttccc ttccgcacaa ggggtgcact gaaaagtcag acgagtttgt
      481 aatggcgtga ctcagctttt cataaaaaaa cttgtcaaag tacgtgtcaa gtatttggat
      541 ttgttttttt tcagtgtaca acgcgtacgc tttttcgact tcactgccta ttccgattcc
      601 cctaagcgtg tttattgact ccgctacgtc ctttgccaca agtgccttga ggatgatgtc
      661 ccgctcgttg attagctctt cggcatgcaa gctgagcatg ccttctattt cttcctgcgg
      721 cctgtcaaga atttttccct ttattatcag cttgaggttt cttaaaatga actttagata
      781 atatgcaaaa agcagagtcg agccgccaac tgacttgatc atcttgtagt gcatttctgc
      841 ctgtctgtcc cttagtgcaa gctcgatttt ttgcgacgtg tagggttttt ggacctttga
      901 tattgtctca ttgtatgcag tgttttttat tctggtgaca agctcgtcta ggtctcttgc
      961 ttcggctagg gtctgaaagt cttttcttgt tagcagtttt cctttgaggg aataagattt
     1021 gactccagca tagactagta atgcagctgt aaccattctc tctcctacct cataatgatt
     1081 ttaattgttt agtataaaaa cgaaaagaaa tgtaagaatt tcagctatgg ttactataat
     1141 tatattattt acgtgatttt tttgaaaatt tttaatccaa aaaacataca aaactaaata
     1201 tcaaattccg gcctttctaa ttgatgccaa tttctgagct gtatgacata aatccaaaat
     1261 cttggatgaa gaagtttcaa aaaaattcca ttctatatct tgtaaaaatg gcagtttttt
     1321 atcatcttct cagtattggg ctgacctatg taggctcgca gcttgtggtc cgtgtaatta
     1381 ccgactatca agaaccatcg tttccagttt caattgtgat ggcactgact tcgggaccaa
     1441 tagaggaggt cttgtttttt ggcttgccat attatttgac tggaaatcca tatgcagtgc
     1501 tggttttagg ctcgatttgg tctgcagcac acatcttcaa tacgcaggtt tttcagctaa
     1561 acagcctagg ctatgttact tttcttgcaa caatcccaca tctgtttttc agtctgagga
     1621 cctggattag cggaaagggc tggttcgcaa ttttgtttca cactggatgg aatcttgcat
     1681 ttttactatc gtattgtttt gcaggcatta ggaattgttc ggtgtttggt gaaggtgact
     1741 atatggcact ggatcttttt gcacttgggt tggcaggatc attattatct attttatatt
     1801 tattgtattc aaaaaacaaa atctcaaaaa gaaaattcag attcacaatg acaagttctg
     1861 ttgcagcatt tgtagtcttt gagatcttgg tgaacctaaa gtatgtggaa ctgtttttct
     1921 ttaactcttg attgctttgt tctttttctt cagtttttca taaaattcta ttgtggactg
     1981 catgtatcca tcaacatttt ttcgaataat tggcatcttg ccataaagct cctcccaaat
     2041 tttcatgtat tctggtcttc gtacaagcaa aaactcaccg atatacaaca aaatgtctct
     2101 tggtattgaa tttggttctc tgagtgccgc aatctggtcc tcaactcctg caaaataatg
     2161 tacatatttt ttgaacaagt tcttgacgtc tcgtgatctt gcaatgtcat ttagatcaaa
     2221 gccttcatct tttaactctg atagaaaatt atcaaaacgt atccatttta aaaaataatt
     2281 ttcaagtctt gcctgaagaa tcttgtcgag gcggtaaaaa tgaatcatgc ccaacagggt
     2341 tgggccgtag tgaatcttgc ccctgctttt gtcctcgaga tttgtgtaaa ctttgatctt
     2401 ttttgtctgc atcatctccc gaaagtgcct atgaatcgta ccaatcgggc ttgaagattt
     2461 ggcatttgcg acataaagtg tcagcggacc ttcctcgagg atggtcctaa agagactgat
     2521 ttgggtctgc tcgtgcgagg acatatccat ttctaactac ctatatgata ataatggata
     2581 tatctatttc tgtggatatg ttatacatgc agctaaaaac atttgctgtt ttgttatttg
     2641 caatgctcat cttcacaaat attcctgcat ttgctgcaca gctaagctgg gatgcaagat
     2701 catttgatag tgcagttgca cccaagttta ccttccagcg gaccgtctta attgagtatg
     2761 aggatggtgg aataattgca gatgatctta ggggaaaaca aacattggtc caattcagcg
     2821 cggatagatc aacaaaagga gtggaaggac tagtacaaaa aataaacgag aatctgcaac
     2881 aaagtggcag tactgtgtgg gtctcagatc ttacgttaca gtattcagct gagctaattg
     2941 gaagagaaag cagcacaact attgattata aaataacaat gattccgaca attagcggat
     3001 ttctgattcg tgagtacaac gaagatgtgt ctgctttgtt tgatgtaaac tggagaggga
     3061 tcaaagtgga tgacccaata gtattatctg attctgagca agaaattgag atcaacagac
     3121 caatctcatt cttgaaaaaa gaatttccta cagtatatga tgtgattacg gaaacaggat
     3181 ctgaaaaaat acttgaattt agtatgattg atgcgtctgg gataaaacaa ctaccaattg
     3241 ccaaatggca ctctttgact gatccgacgg caattatatc agatacctct caatatggat
     3301 tcaaaggcga agtgttaact gcattctcaa tgggtgagag cacgattttt acaccaacta
     3361 aagaaaagtt ttcagagata gtctttgtgt cagacaaaga atacaaaata caaactcatg
     3421 aggcagcaga caatgcaaac ttgtttgtcc caggctatgc cgtgccaacc atgattgtcg
     3481 agcacgaagc aattggagca agtccaacgg cactatctgg tactcctcca agccagcagg
     3541 gacaattccc aatatttgtc atatatggga tggcaggcat ggcagcagca ggagctggcg
     3601 gctttttttg gtggagtagc aaaaaggcca aaaaagatct caagttgggt caatctggaa
     3661 ttgatcctac attactaagg ggagttgaga caagctcggc ttctggtgga tataagacca
     3721 acaggggtga ggcacatcta gttggagata ctatgtatca acaaaccaaa agtgtgtatg
     3781 ata
//
LOCUS       DASWKH010000112         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3253585, whole genome shotgun sequence.
ACCESSION   DASWKH010000112 DASWKH010000000
VERSION     DASWKH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3253585"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VGA92_08465"
     CDS             complement(<1..922)
                     /locus_tag="VGA92_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX9846474.1"
                     /translation="MLDGLKNNLRAAIKKLVSSSGVDEELIKELARDVQRALLQSDVN
                     VKLVLEITKNLEHRSLNETPPPGLSRKDHIVKILYDELAKLLGNETEFSFKPDKLNKI
                     LMLGIQGSGKTTITAKLAKFLSKQGYKVGAVGADTFRPGALVQLKTMCEKANIEVYGE
                     ENNKDSPSVVKNGIKHFTDSGIDIVLIDTAGRHKEEKDLLDEMKQIGKAAEPDLALLV
                     IDGTIGQQCYSQAEAFHKTVPVGGIIVTKLDSSAKGGGALAASAATGAQIMYVGTGER
                     IDDLEKFSPTRFVGRLLGMGDVQALLDLAKR"
     gene            complement(915..1604)
                     /locus_tag="VGA92_08470"
     CDS             complement(915..1604)
                     /locus_tag="VGA92_08470"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEX9846475.1"
                     /translation="MKLAALFSGGKDSTYSIYSAKKDGHQISCVVTVFPISFESELLH
                     FPNIRLTKLQSQAMGIPQLSIDADSTNPNSEVSTLQEILYKAKENFGVEGILHGGILS
                     EFQKKRFSSICENLNVKLVSPLWKKNQKEYMQNLLKENFHFIIISVSADGLDESWLGK
                     EITQDDLKKLEALSEKYGFNLSFEGGEAETLVTDCPLFSNPIKIKKSNKVWDGYRGRF
                     EILEAELAHNA"
     gene            complement(1737..>1963)
                     /locus_tag="VGA92_08475"
     CDS             complement(1737..>1963)
                     /locus_tag="VGA92_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214912.1"
                     /note="eIF-5A; promotes the formation of the first peptide
                     bond; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HEX9846476.1"
                     /translation="VGPVSMQVHVPLIDKRMGQIISITDSAIQVMDSETFETLDMNMV
                     EDELKGKIQQGQDVEYWKVMDRTKIMRIKS"
BASE COUNT          529 a          408 c          321 g          705 t
ORIGIN      
        1 gtctttttgc caagtctaaa agtgcttgaa catcacccat gcccaaaaga cggccaacaa
       61 atcttgttgg agagaatttt tctaaatcat caatacgttc tccggttccc acatacatta
      121 tttgtgcacc tgttgcagca gaagcagcta aagcgcctcc tccttttgct gagctatcaa
      181 gttttgttac tattattcct ccaactggaa ccgttttgtg aaatgcctca gcctgtgagt
      241 aacattgctg accaatggta ccatcaatta caagcaatgc caagtcaggc tcagcagctt
      301 tacctatctg cttcatctca tcaagcaggt ctttttcctc tttatgacgt cctgcagtat
      361 caataagaac aatgtcaata ccagagtctg taaagtgttt aatgccattt tttacaacag
      421 atggcgaatc tttgttattt tcttcaccat aaacttcgat gtttgctttc tcacacattg
      481 tttttagctg aactagtgcg cctggcctga aggtatctgc accaactgcc cctaccttat
      541 atccctgttt tgagagaaac ttggcaagtt ttgcagtaat tgttgttttc ccgcttcctt
      601 gaattccaag catcaatatc ttgtttaatt tatctggctt gaatgaaaat tcagtctcat
      661 tgccaagaag ttttgctagc tcatcgtaaa gaatttttac aatgtggtct tttcgtgaaa
      721 gacctggagg tggagtttca ttaagggatc tgtgttctag attttttgtg atttcaagaa
      781 caagttttac atttacatcc gattgtagca aggcacgttg aacatcacgt gctagttctt
      841 tgattagctc ctcatcaact ccagaagagc tcactagctt ttttattgct gctctgagat
      901 tgttttttag gccatcaagc attgtgagct aattctgcct ccagtatttc aaatcttcct
      961 ctgtatccat cccatacttt gttagatttt ttgattttga taggatttga aaataacgga
     1021 cagtcagtta ccaatgtttc tgcttcccct ccctcaaagc ttaggttaaa tccatatttt
     1081 tctgacaacg cttcaagttt ttttagatca tcttgtgtga tttcttttcc aagccaagac
     1141 tcatcaaggc catctgcact tacactaatt attataaaat gaaagttttc cttgagaaga
     1201 ttttgcatgt attctttttg gtttttcttc cacagtggcg aaacaagttt tacattcaag
     1261 ttttcacaaa tacttgaaaa tcgcttcttt tgaaattcac ttaatattcc accatgaagt
     1321 attccttcaa ctccaaagtt ttcctttgcc ttgtaaagta tttcttgaag tgtactaact
     1381 tctgaatttg gatttgtaga atctgcatct attgatagtt gtggtattcc cattgcctgt
     1441 gattgtagct tggtcaaacg aatgtttgga aaatgcaaca gctcactttc aaaagatatt
     1501 ggaaatactg ttaccacaca gctaatttga tgcccgtcct tttttgcgct gtatatggag
     1561 taagtactat ctttaccacc agaaaatagt gctgcaagtt tcattatgat aagaaattct
     1621 agatgtaatt ttaagtgtca ataacaaatt tgcaggggaa aagcaccgga tgcttcaatt
     1681 ctcataatct tcatcaaaaa tcagatggct tttccaaatc atcatcagca tccaaattaa
     1741 gatttgattc tcattatttt agttctatcc attactttcc aatattcaac atcttgacct
     1801 tgttgaattt tgcctttgag ttcatcttct accatgttca tatccaaggt ctcaaaggtt
     1861 tctgaatcca ttacctgaat ggcagagtct gttattgaga ttatctgacc cattcttttg
     1921 tctattaatg gcacatgtac ttgcatacta acaggaccta cgt
//
LOCUS       DASWKH010000113        21072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3254826, whole genome shotgun sequence.
ACCESSION   DASWKH010000113 DASWKH010000000
VERSION     DASWKH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 21072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21072
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3254826"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGA92_08480"
     CDS             <1..236
                     /locus_tag="VGA92_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9846477.1"
                     /translation="IRSTTHIIPIVIGDEKTTLEFGKYLYDGGVFAQPIRYPTVAKNS
                     ARIRVSVTAWLSKKQIEHALYTFEKAGKKFRIL"
     gene            434..1168
                     /locus_tag="VGA92_08485"
     CDS             434..1168
                     /locus_tag="VGA92_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9846478.1"
                     /translation="MPDITIAVAALAGLGSFLAPCILPVIPAFLAYISGTTITDLQRN
                     NGTVNLLSNRLNVLMNTIFFVLGFSVVFSVFGVILNGVLSNSAASLTLGFNQVGGIII
                     IGFGVFMLLSTKITKMNFEKKFFPKGGRASYPMSFVFGLAFATAWTPCIGPILGSILT
                     LAATTPSQAFTLLLAYSLGLGIPFILMGVFFSRMTRVIRALSKHLKYYSVIMGAFIIL
                     LGVLVLTNQLAAIASFPFLNDLILLG"
     gene            1174..2298
                     /locus_tag="VGA92_08490"
     CDS             1174..2298
                     /locus_tag="VGA92_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX9846479.1"
                     /translation="MRSEFKTAIIMGVIVVGAIGGLSAYFTSLDQSAMDKTDGLKTQT
                     TQENSALPNIDKSRFKKAPELEGINGYINTNSEEFKEQIEGKVVLYDIWTYSCINCQR
                     TLPYLTAWDEKYSDEGLLIIGIHSPEFEFEKDINNVELAVEKFGINYPVILDNDKTIW
                     KSFENRYWPRKYIADDEGYIRYDHIGEGAYDETEKIIQNLLQERATRLGLNVASAQSL
                     VDLEEFKHGARTPELYFGHNFAFGRNQLGNSEGFKPQQDVKYVIPQSLKDNFFYLDGT
                     WKNLEDRMILVSESGTIKLPYFAKQVNIVADGNSELTILLDGKPISQSDIGDDVSQVG
                     KVVISEATLYNLVNTPESSKHTLEIQVNEPGFEIYTFTFG"
     gene            2386..3042
                     /locus_tag="VGA92_08495"
     CDS             2386..3042
                     /locus_tag="VGA92_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846480.1"
                     /translation="MGSISDKWSDSGRTDNLSQRILDKVKPDVPLKNKIDVAQKNLQL
                     QIIKLDEIGIKIKQKHDYIFNKIVSAQKSNNHLHAKAYATELLEIRKMHNMVNGAKLS
                     LEQIQLRLNTVSELGDIVVTLSPCMSVIKGLGASLSGIMPEATSSMQNLSQILGDVLT
                     GSSMNATSTTVSTYSPSADTLAILEEAQAVIEGQARTNIPEPPSGTPSSVMQKKETLI
                     "
     gene            complement(3035..3376)
                     /locus_tag="VGA92_08500"
     CDS             complement(3035..3376)
                     /locus_tag="VGA92_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846481.1"
                     /translation="MEEIQIKLQLTKKPTDTQLRAIEDCFREIPLEEILTGLKFAKNR
                     WSAKDAGILKVGRKSIIQKEVHSVTNEQAQWRLKNWKMMISNYRRRGYSYPTISRIKK
                     ILIEKSKKKSR"
     gene            3529..3960
                     /locus_tag="VGA92_08505"
     CDS             3529..3960
                     /locus_tag="VGA92_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846482.1"
                     /translation="MQDRHLERKEARKEYDPLLDKPERELFFQHEEREAEKASAEKAR
                     DKVVEAGNDTRNTNQYVKLVNAHIQNKVSTMQRIKEAQKKFEAEVDMFKVNSEADEQP
                     QAMPTQSSDVESMLIVKELSIMINKYGIEKISKALDQILKP"
     gene            4330..4614
                     /locus_tag="VGA92_08510"
     CDS             4330..4614
                     /locus_tag="VGA92_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846483.1"
                     /translation="MNSRTEQHKLALLSCIELVLMRRGNTDYNLVVAKLDSLYNCTIR
                     DCYEHPDYLRTILKDVYNDEYNNIIDEIKVQLDELTNVEEIRDFCQSLAS"
     gene            complement(4927..5472)
                     /locus_tag="VGA92_08515"
     CDS             complement(4927..5472)
                     /locus_tag="VGA92_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846484.1"
                     /translation="MADAKSTWGSRFIISAIVQGGIITFLALSMVGIQVIFAKDINMI
                     QFLSLSFEGPAKWFFIGLMLYLILTVAIAVTAVFYNHLEINLKKRIFGKIRILVWVHL
                     LGMNIGGIGTTLSMIFAGLAGSGALSMVTEGQLGQQNIAIMDSFIPPIATFIGILAIG
                     VLCGGLAYTTIYVKKSYTISE"
     gene            complement(5535..5783)
                     /locus_tag="VGA92_08520"
     CDS             complement(5535..5783)
                     /locus_tag="VGA92_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846485.1"
                     /translation="MARIRRANRYCIDCGGRLTYYPRLSNPKAPNEHRVLVYACPDCT
                     DDFEKPKLLSIRRNQVEDPLETVEIEIIEPRQKISLSN"
     gene            complement(5985..6058)
                     /locus_tag="VGA92_08525"
     tRNA            complement(5985..6058)
                     /locus_tag="VGA92_08525"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6022..6024),aa:Asn,seq:gtt)
     gene            complement(6122..6679)
                     /locus_tag="VGA92_08530"
     CDS             complement(6122..6679)
                     /locus_tag="VGA92_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX9846486.1"
                     /translation="MSTKQAEIFESTYEIPQKVKVSLKKHMLHVEGPLGKTYKNFKKI
                     PVELEVKDNKVSIKAVNSRKKYSAIANTASSIIRNLCEGVVEGYTVKMKIVYAHFPIT
                     VKTKDDKVLIENFQGERAPRVARIHGSTKVVSKGDDVIVTGSVLNDVTQTAAEIQTKT
                     KVKGKDHRVFLDGIYLYYKGKGIEK"
     gene            complement(6669..7061)
                     /locus_tag="VGA92_08535"
     CDS             complement(6669..7061)
                     /locus_tag="VGA92_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX9846487.1"
                     /translation="MPATNIISNIFNTLYNNEARRRTECVILPTSKLAIEILKTLQKH
                     NYIGEFEHVEDNRGGKFKIKLLAHITKCGSITPRFRVKKNEYADWEQQYLPAYNRGML
                     LVTTNKGVMSHHEAENNGIGGFLIGYVY"
     gene            complement(7066..7242)
                     /locus_tag="VGA92_08540"
     CDS             complement(7066..7242)
                     /locus_tag="VGA92_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX9846488.1"
                     /translation="MKDRSYKYTGRKEHKFGKGSRWCKRCGDYTAVIQKYNLMICRRC
                     FREVADSLGFIKYR"
     gene            complement(7239..7763)
                     /locus_tag="VGA92_08545"
     CDS             complement(7239..7763)
                     /locus_tag="VGA92_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX9846489.1"
                     /translation="MADNQENPMRIIRLEKLVLNMGVGKSGDAIVIAKKALDSITGKK
                     SCARDAKATQRDWGVRKGEPIGVAVTVRGQDAATILKRLLDAVGNKIKARSFDDFGNV
                     SFGIKEHIDIPGIKYDPQIGILGLEAAVTLTRPGFSIRMRSRHKASVGKKHRISKEEA
                     TEFLSREFGVSIVQ"
     gene            complement(7768..8484)
                     /locus_tag="VGA92_08550"
     CDS             complement(7768..8484)
                     /locus_tag="VGA92_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HEX9846490.1"
                     /translation="MTRIAGSKKLKRQMAPMFWGILRKRPRFVTTVRPGPHSKHFSIP
                     TAVFLRDTLKLVTTAREAYYAIYNGKVKIDGVKRKSIHHGIGLMDVIELDGVSDIYRL
                     VPKQGHLLEPIKINSSEKSIKLVKVTSKTTIKKGKLQLGFHDGRSLISDIDASVGDSC
                     VIQVPEQKINDVLNLKEGVKVIVTKGINAGQVAEVKEIKEGTFVLPKRVLLSLEQKEI
                     EIPADLVMVVGKKEPVIQIA"
     gene            complement(8484..8975)
                     /gene="rplX"
                     /locus_tag="VGA92_08555"
     CDS             complement(8484..8975)
                     /gene="rplX"
                     /locus_tag="VGA92_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX9846491.1"
                     /translation="MKPSIIRNRTIFKASLAVKSRLLCSHLTKDLQNKYSRRSVRVTE
                     GDTVRVMRGEYKGVSGKIIKVSSEKNGVAVEGIKREKLKGGNVDVFIHPSNLLITDLN
                     TEDKWRQNKLEGKHQKSKETKPKEEKPKTTPQKEAKQEVKQEKPKENKESKTAKNAKK
                     EDK"
     gene            complement(8977..9414)
                     /locus_tag="VGA92_08560"
     CDS             complement(8977..9414)
                     /locus_tag="VGA92_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX9846492.1"
                     /translation="MSTGKSRAVSAKGVQEFRPYITRALPLGARITCADNTGAKILEI
                     IMVKRAKTRTSRMPSAAVGDFVNVVVKKGPAELRKQIFGAVIIRQKYPIRRLNGVRVA
                     FEDNAGILVTPEGEIKGTDIKGPVAAEASEKWPRVANLASMVV"
     gene            complement(9416..9721)
                     /gene="rpsQ"
                     /locus_tag="VGA92_08565"
     CDS             complement(9416..9721)
                     /gene="rpsQ"
                     /locus_tag="VGA92_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX9846493.1"
                     /translation="MSKDAQKHVYKGLVMDNGKSLSVRGKLFEGKVISAKNKNTVVIR
                     RENPLYITKTKRYARSTSTIHAYKSANLDLKEGDVVIAAECRPIAKSVAFVVVEVRS"
     gene            complement(9718..9984)
                     /locus_tag="VGA92_08570"
     CDS             complement(9718..9984)
                     /locus_tag="VGA92_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HEX9846494.1"
                     /translation="MITQQNIINHELLGLDAQILESTNKAVVGLLGKVIDETKSMLLL
                     DTKRGTKMVSKQHNKWKFTLGDQDFTVDGALISKRPEDRIKVKA"
     gene            complement(9981..10184)
                     /gene="rpmC"
                     /locus_tag="VGA92_08575"
     CDS             complement(9981..10184)
                     /gene="rpmC"
                     /locus_tag="VGA92_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX9846495.1"
                     /translation="MARLKMKSIREFNEMDLKERLGQSRTELTKLRTDAAKGTLRKES
                     GKIKALRKEIARMLTRLNELKRK"
     gene            complement(10184..10924)
                     /locus_tag="VGA92_08580"
     CDS             complement(10184..10924)
                     /locus_tag="VGA92_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX9846496.1"
                     /translation="MSSLKNVIKDNYNMMLLKEYLKTAIKESGFSHVDISKTPIGTRV
                     VLHVTRPGIVIGRKGAGIRELTEKLEKDFGLKSPQIAVNEITEPELTASVMCNRLASL
                     IERGTAFRRATMWTLQQIMNAGALGVQITVSGKLRGDRSSFEKHSAGILPRAGHKATL
                     AVDEDNVHVPTPMGYIGVRIRIARKERYLPDFELKGKEELAAQERKLAKEREDAEVKA
                     RTESEQIKIDAERIEKLDLMEEIEEELK"
     gene            complement(10927..11385)
                     /locus_tag="VGA92_08585"
     CDS             complement(10927..11385)
                     /locus_tag="VGA92_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX9846497.1"
                     /translation="MPQFGYAFQNFDPTKHVRAAVREKSVSHKHAREIAKMIKGLSIE
                     KARDYLQEVISLKRAVPFRRYKNEVGHRSDTGVMSGRYPRKAATEFIKLLDNLEANAE
                     YKGMDLDRLKIVGANTHRGVLIKRFTPRAMGRATPKNNVLTHIELVAQEK"
     gene            complement(11393..11788)
                     /locus_tag="VGA92_08590"
     CDS             complement(11393..11788)
                     /locus_tag="VGA92_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX9846498.1"
                     /translation="MVKEFDYRGIPLEQLQSMSLEKLFEIFPARARRSLTRGITDGKR
                     KLIEEIKLEKAGKSKNPIKTHIRDLIVLPYMVGVTINVFSGKEFLPVTITPQMIGHYT
                     GEYVRTNKRVVHGAPGVGASRSSLYVPLK"
     gene            complement(11827..12093)
                     /locus_tag="VGA92_08595"
     CDS             complement(11827..12093)
                     /locus_tag="VGA92_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX9846499.1"
                     /translation="MNATQATKIILRPHITEKTFALVEKDSRICFIVDPQANKKTIKE
                     AIKTLYEEDVVKINTARTISGKKAFVKFESVEKARDLATKIGML"
     gene            complement(12090..12899)
                     /gene="rpl4p"
                     /locus_tag="VGA92_08600"
     CDS             complement(12090..12899)
                     /gene="rpl4p"
                     /locus_tag="VGA92_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX9846500.1"
                     /translation="MKAQTLSLSGTKQEEIDLPKVFSTPFRKDLIHKAYVNLDSHHYQ
                     RQGRHPTAGMDVVAKSNDPPTGHGQARIARIRGGGPRRGEAAGVAMARGGRQAHPPTS
                     EKVIYKKLNKKENRLALCSAIAATTSKDIIASRGHIVDNITSFPIIVSDDVEKISKAK
                     EISKVFDALNLAKDIQRLQKRKARSGRAVIRGRKTKTGKSILFVVKDAKSLAKACGSF
                     LGVDVREAKDLSVLDLAPGSQPIRLTVYTKAAIGEIANIKSPHLQLMEAIQ"
     gene            complement(12896..13888)
                     /locus_tag="VGA92_08605"
     CDS             complement(12896..13888)
                     /locus_tag="VGA92_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX9846501.1"
                     /translation="MGHRKHSQPRRGSLAYLPRGRAKSMEARIRTWPEINSEQPKLLG
                     YAGFKVGCIQIVSIDDREKTPNHGKQLVSLGTVVATPPVAVIGIRGYHEDVDGTHALF
                     DVFSNDMPKEVSRLITIKPKEGSLEKAEKLLNRISDLFAVVAVSPNNIGLAQKKPYVF
                     EVAVKGGDIKKQFDFLKGIFGKQVKVEDVFELGSTVDVASITKGKGWEGPITRWGVKK
                     KQHKSRKSVRALGTLGPISPSSIMYTVPRGGQRGFHQRVEYNKRIMIMSNTEKNEFKI
                     NPTGGYKHYGLVDGDFVVLKGSIPGTYRRLVKLRTQIRNIQSKILKPNILEIVV"
     gene            complement(14073..14888)
                     /locus_tag="VGA92_08610"
     CDS             complement(14073..14888)
                     /locus_tag="VGA92_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA uridine N3 methyltransferase"
                     /protein_id="HEX9846502.1"
                     /translation="MNISVAIPDSSLKDEATKLDKSRKVSSIARACAIFSVNTIFVYE
                     ESGGNYSDRFLLSTVLKYLETPQYLRRTLFPKIDELKYAGILHPLQIPHHSLSSNPKN
                     LKEGDIREGAIVHYRGKKFIDIGVHKLIPYFGKEHEKKRITVQIKTVEPELTAKEISE
                     FHIKQYWGYKVKERPNLVSILEEWNGNVILTSRKGKEATAQQIREYASSDKPLLIVFG
                     APDRGLYDILGKKINEVHNARVLNFFANQATETIRLEEAVLGILSILNFGNSN"
     gene            complement(14901..15638)
                     /gene="psmA"
                     /locus_tag="VGA92_08615"
     CDS             complement(14901..15638)
                     /gene="psmA"
                     /locus_tag="VGA92_08615"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HEX9846503.1"
                     /translation="MLPSQQGYDRAITIFSPDGRLYQVEYAIETVRRGAIAVGVKCKD
                     GVVIAVEEKPRKLQISESAHKIFQIDEHIGFAAAGYIPDARSQADTARFFSQSNRIVY
                     DEAVDVETVAKHLADQCQQFTQFGGARPFGVALIIGGVDSSGNSLFLTDPSGTYIAYD
                     AVAIGANSEQANDFLEKNYKPDMSLEDASALATACIYIASDQKEGTDHIKMSHIKTST
                     KQFEKVTIEQIANYAKIGKQKFPIPNV"
     gene            15691..16107
                     /locus_tag="VGA92_08620"
     CDS             15691..16107
                     /locus_tag="VGA92_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HEX9846504.1"
                     /translation="MRFEIPFYGHKNVRALHPRTIEITTEPDLTLDGDCIIGVSADCG
                     CKEIPKKLKQKLRQTDSKITITIRVKGKSFTITGNGHPDLRLENPHDIVIRKSNFLCP
                     RTLAVGCDLASNDMPRQMIKLLQDPKTKGLFVIEVT"
     gene            complement(16100..16618)
                     /locus_tag="VGA92_08625"
     CDS             complement(16100..16618)
                     /locus_tag="VGA92_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX9846505.1"
                     /translation="MSTLLEKQIKINRVMTISVEQARALEDPARSKIVEILYHKQLTT
                     DQITKELQKNGCNKAVTTIRHHLEILKEAGLIEIVKIQEIRGAIAKFYGTSIKLIANT
                     SSKDFDVKYSSMIKSTSTKIEKILDGIAKKPALQKKSESVHNEFVLMEIVNRAMANVL
                     ENNNFTFQKKIK"
     gene            complement(16926..17750)
                     /gene="purC"
                     /locus_tag="VGA92_08630"
     CDS             complement(16926..17750)
                     /gene="purC"
                     /locus_tag="VGA92_08630"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9846506.1"
                     /translation="MKFLGSGKVKDVYDLEDGNLLFKFSDRVSAYDVKFHDPIPYKGE
                     VLAKFAEFWFTKIPVKNHFVKTVSKNEIVVKKMKMIPVECVVRGYFYGSLITRWKASQ
                     VSLPAGTQTEIAAKLPVPIFDPTTKAEHDEPIDKQTATEKGLVTETEYNWLELKSIEI
                     YNQMYGVADAAGFILADLKLEFGKLDDQIILGDSIGPDEYRLWPKEIYAVGKIQEAYD
                     KQILRDWLTANGYQQQFDDARKQGKMPVAPSIPQEIIQKMSERYIISYERISGKPL"
     gene            17820..19364
                     /locus_tag="VGA92_08635"
     CDS             17820..19364
                     /locus_tag="VGA92_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9846507.1"
                     /translation="MPTLRILLLVLGLSLGFVPVFADEPQIKADDLVVETFVDNIPNN
                     PTTMTFVGDDILVLQRYNGQVRLVHDGVLQDKPVLDVSVAKDGERGMLGITSIGSTVY
                     VYFTAADTDGGKAIENRIFKYEWNGSELVNPILLKQLPGDNYYHNGGAMTNFDGQVYA
                     VIGDNGNYGKLQNRQSNWKNDTSVILRVDPPGPYYAMGIRNSFGITFDPFTGNMWDTE
                     NGDDDFDEINLVPESFNSGWIEIMGPAKNQSQIDSLPKYGNFVYSDPEFSWQKPVAPT
                     GISFVKSEKMLDYQDSVFVGDCNTGNLYRFKLNSDRTDFVFETPELSDKVLNLSDPSD
                     EIIFGSGFGCVTDIELGPDGLLYIVSLSEEKIYRLVPKSMAQASQEPEIVDGGCLIAT
                     AAYGTELATQVQLLREVRDNVVFSTHSGTTFMTGFNQAYYSFSPTISDWERQSPVFRE
                     MIKIIITPMLSTLSLLNYAEIDSEQEMLGYGIGIILLNVGIYFIAPVILIEKLRKLID
                     KCNRKS"
     gene            19520..>21072
                     /locus_tag="VGA92_08640"
     CDS             19520..>21072
                     /locus_tag="VGA92_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846508.1"
                     /translation="MAGLVLEIAITRIFSAAVSYHFAFVAVSVALVGLGSSGLLVHYR
                     LKKIKENWASDLAILSSLGVTLVIPLTLFVMHYFASRVMYLPLYMLLFAVSFFFIGTV
                     ISAAFSAFAHVAGRLYAADLIGASLGAVSVVLLLSMLGGEGTVLTVGFISAICATVFS
                     IVAKNKKRVLISLAFVAFAAGLLFINETIEIFSIQTNPDAQKDLPLFLKENPGAHIVK
                     TQWNSFSRIDVVEGITGNCAGSSDPYAQRQCSQKDIVAKIFIDGGAGTNIIFWDGNPD
                     SRQSLSSWMQYVPFKMKDDPKVLVIGSGGGRDVVAALASGSRDVTSVEINPIIFETVK
                     SYGQKAGNLYTHPYVHSHIDEGRSFISKSQEKFDIIYIPFVDTWASVSSGSLGVSENF
                     LYTIEGFQEYYDHLEDNGKIIAVRWLIDSPRFVSTFAELLEQNGLSRTELQKHLVIVT
                     AESTEKDPSVTMVIFSKSPFTENEVQFLSDSFERNGYKPILMPDKIAPEKYSGFLSDK
                     LSHQEFYAL"
BASE COUNT         5788 a         4281 c         4088 g         6915 t
ORIGIN      
        1 acataagatc aactactcac atcatcccaa tcgtgattgg tgatgaaaaa accactttgg
       61 agtttggaaa atatctgtat gatggtggag tttttgccca gccaataaga tatcccacag
      121 ttgcaaaaaa tagcgcaaga atacgagtat cggttaccgc ttggctttca aaaaaacaaa
      181 tcgagcatgc tctctatacc tttgaaaaag ctggaaaaaa gttcaggatt ctctaattat
      241 ctgcgcaaat catagccaag aaacgcaggt gagccgacat aaactgcaat tataactatt
      301 acagccaata tgacaccaat tatgatgaat cgtttgttca cgaattattg ccaaatatgg
      361 ccagataaaa acaataccaa attggagtaa atttggaaca atgctttaat aaaaaattga
      421 agacttgatg agcatgccag atatcacaat agcagtggca gcactagctg gacttggatc
      481 gtttcttgca ccatgcatac tgcccgtaat acctgccttt ttggcatata tctctggcac
      541 aacaattacc gatcttcagc gaaacaacgg cacggtcaat cttctctcta acagactgaa
      601 tgttttgatg aatacaatat tttttgtcct aggattttcg gtagtgtttt cggttttcgg
      661 agtaattttg aacggtgttc tttcaaacag cgcagccagt ctaactttgg gctttaacca
      721 agttggcgga ataatcataa ttggatttgg cgtattcatg ctactgtcta caaaaattac
      781 aaagatgaat tttgaaaaaa agtttttccc aaagggtgga agggcaagct accccatgtc
      841 ttttgttttt ggacttgctt ttgcaaccgc gtggacccca tgcattggcc caattttggg
      901 aagcattctt actttggctg caacaacacc aagccaagca ttcacgttgt tgcttgctta
      961 ttcacttgga cttggaattc catttatttt gatgggggtc tttttctcaa gaatgacgag
     1021 ggtcatcagg gctctaagca aacacctaaa atattattct gtgataatgg gcgcattcat
     1081 aatactactt ggggttctag tactaacaaa ccaacttgca gcaatagcaa gctttccatt
     1141 tctaaatgac ttgatattat tgggttgatg aatatgagat ctgagttcaa aacagcaata
     1201 attatgggag taatagttgt cggcgcaatt ggtggtctga gcgcatactt tacctcactt
     1261 gatcaatcag ccatggataa gacagatggg ctcaaaacac agacaacgca agaaaactcg
     1321 gctttgccaa acatagacaa aagccgcttc aaaaaagcac cagaacttga gggaattaat
     1381 ggttatatca acacaaattc cgaggaattc aaagaacaga tcgaaggcaa ggtcgtcctc
     1441 tacgatattt ggacgtacag ctgcattaac tgccagagaa cgctcccata cttgacagca
     1501 tgggatgaaa aatattccga tgagggtctg ctcataattg gcattcactc accagagttt
     1561 gaatttgaaa aagacatcaa caatgtggaa ctagctgtag aaaaattcgg aatcaattat
     1621 cccgtaatac ttgataacga caaaacaatt tggaaatcgt ttgaaaacag gtactggcca
     1681 agaaaataca ttgcagacga tgaaggatac ataagatatg atcacatcgg cgaaggcgcg
     1741 tatgacgaga cggaaaaaat aatccaaaac ttgctacagg agcgagctac aagacttgga
     1801 ctaaatgttg catcagcaca atctcttgtg gacctagaag aattcaaaca cggtgcaagg
     1861 acacccgagc tgtattttgg ccataatttt gcatttggga gaaaccagct tggcaactct
     1921 gaaggattca aaccgcaaca agacgtaaaa tatgtcatcc cgcaaagttt gaaagataat
     1981 ttcttttatc tagatggtac gtggaaaaac ttggaagaca gaatgatcct agtatcagaa
     2041 tctgggacaa tcaagcttcc atattttgca aaacaggtta acattgtagc agatggcaac
     2101 tcggagctaa ccatacttct tgatggaaag ccgatttcac agtcagatat tggagatgat
     2161 gtaagtcagg tcggcaaggt ggtcatctcg gaggcaacac tatacaattt ggttaatact
     2221 ccagaatcat caaagcacac attagagatt caagtaaacg aaccgggttt tgaaatctat
     2281 acgttcacgt ttggataggt gtaaccacag tgtcttgtta ccagagtgac atatgttgtg
     2341 gttacatctt tctttaaaac caatttttct agtacctaca ggaaaatggg gtcaataagt
     2401 gacaaatgga gtgatagtgg gagaacggat aatctttcac agagaatact agacaaagta
     2461 aagccagatg taccactcaa aaacaaaatc gacgtggcac aaaaaaatct tcagcttcaa
     2521 attatcaaat tagatgaaat cgggataaaa ataaagcaaa aacacgacta tattttcaac
     2581 aagatagtca gtgcacaaaa atcaaacaat catcttcatg ccaaagcata cgccactgag
     2641 cttttagaaa ttagaaagat gcacaacatg gtaaatggtg caaaattatc ccttgaacaa
     2701 attcagctta gactaaacac agtatcagaa ttaggcgaca tagtcgtaac gctcagccca
     2761 tgcatgtctg taatcaaagg acttggcgcc tcattaagcg gaataatgcc tgaggcaaca
     2821 tcgtcgatgc aaaacctatc acaaatacta ggcgatgtgc tgactggatc atctatgaat
     2881 gcaaccagta caacagtgtc gacatacagc ccaagtgcag acacgctagc aatcctagag
     2941 gaagcgcagg cagtaatcga gggccaggcc aggacaaaca ttccagaacc gccgtctgga
     3001 acaccaagca gtgtaatgca gaaaaaagag acgctcatct agactttttt ttgctttttt
     3061 ctatcagaat ttttttaatt ctagaaatgg tcggatagct gtagcccctc cgccggtaat
     3121 ttgagatcat cattttccaa ttctttagcc tccattgcgc ctgctcgttt gtaacagaat
     3181 gaacctcttt ttggataatg ctttttcttc cgactttgag aatccctgcg tccttggcag
     3241 accagcggtt ttttgcaaat ttgaggccgg taagaatttc ttcaagaggt atttccctaa
     3301 aacagtcttc aattgccctc agctgtgtat cagtcggttt ttttgtcagt tgtaacttga
     3361 tttgaatctc ttccacggca aaaccagctc agtcatttct taatagagtc atgataagct
     3421 agattttaca aaatgttgta tttatcaggc gctaacacat tggtcttttt gtttgttcga
     3481 taacgacaga ataccccata ttacgtaaaa tacttaacaa ataggcatat gcaggacagg
     3541 catctggaaa gaaaagaggc tagaaaagag tatgacccgc tcctagacaa gccagagcga
     3601 gagctgttct tccaacatga ggaaagagag gctgagaaag ccagtgccga aaaagccaga
     3661 gacaaggtcg ttgaggccgg aaacgacaca cgaaacacca atcagtatgt aaaactcgta
     3721 aatgcccaca tacaaaacaa ggtttccaca atgcagcgaa tcaaggaagc acaaaaaaag
     3781 tttgaagcgg aagtagacat gttcaaagtc aactcagaag ctgacgagca gccgcaggcc
     3841 atgccgaccc aatcaagtga tgtcgaatcc atgttaatag tcaaagaatt atccatcatg
     3901 ataaacaaat atggaatcga aaaaatatca aaagcattgg atcaaatact caagccctag
     3961 caaaaacacg cagaacacac caaggaatta gaaaaaacag cccttcgatt ttgctcattt
     4021 ttaattgtca acgtcttcaa aaatagtatc tttctaaatt cgaattttga aacattggat
     4081 ttttgagcct gagattatga tatcaactaa cctcaagtca tccacggatt ccatcattca
     4141 agcagaattc aagaaacaaa atttagttat ccaaaagcat gttggtggac gatcagggta
     4201 cagaaggttg tcttaaaatg atttattaga ttggttttta cgaaatgaca tatcatgata
     4261 ttccctaatg aagttacttg gtaggttttg tccaacgatg gcagatataa cataaggaga
     4321 ttctaaatta tgaattcgcg tacagagcag cacaagctag cattactgtc ttgcatagaa
     4381 ctggtgttaa tgagaagagg aaataccgac tataatctgg tagtagctaa actcgactcc
     4441 ctttacaact gcacaatcag agattgttat gagcacccag actatttgcg aaccatactc
     4501 aaagatgtgt ataatgacga gtacaataac attattgatg aaatcaaagt gcaactagac
     4561 gagctaacca acgtagagga aataagggat ttttgccaaa gtctggcgtc ataaccccct
     4621 acttttccat caaaacaaga cacaaaacaa ctactagtcc caaaaatctc gaaaaaaaga
     4681 accaaaaatc aaacgggagg attttgttag aaaaagtggt tttgttcgca aaagctaaac
     4741 cccctcccat aggatcccca tttacgagta atatgatttt ttcaaaaacg ccaatttttg
     4801 cggggggttt agcgtttatg cgtaactggt gcaggcaacc atcaaatcac ccagatagat
     4861 ggccaaaaca tagtcaaact atttttctta cgaagaaaag ctaaacccca ccaaaaaggg
     4921 taattttcac tcagaaattg tatatgattt tttgacgtaa attgtagtgt aggccaagcc
     4981 gccgcacaga acgcctattg ccaaaattcc aataaatgtc gcgattggag gtatgaaaga
     5041 atccattatt gctatgtttt gctgaccaag ttgcccctca gtcaccatgg aaagtgcgcc
     5101 agagcctgca agtccagcaa atatcatgga taaagtcgtt ccaattcccc caatattcat
     5161 acccaaaagg tgtacccaca caagaatccg gattttacca aatattctct tttttagatt
     5221 tatttcgaga tgattgtaga atacggcagt tacggcaatg gcaacagtca gaatcaggta
     5281 cagcatgagc ccgataaaga accacttggc cggaccttca aaagacagag atagaaactg
     5341 gatcatgttg atgtctttgg caaaaattac ctgaatgcca accatactta atgccaaaaa
     5401 cgtgataatg ccaccctgaa cgattgcaga tattatgaac ctgcttcccc aggtgctttt
     5461 agcatctgcc acatgtagtt tttgaagtgg ttgaataaag tcttatttgt tagagcaagt
     5521 aaactgaaaa tcaactaatt ggacagcgat attttttgtc ttggttcgat tatttctatt
     5581 tcaacagtct ctagaggatc ttcgacctga tttcgtctaa ttgacaaaag ttttggcttt
     5641 tcaaagtcat ctgtgcagtc agggcatgca tataccaaaa ccctgtgctc atttggcgct
     5701 tttgggtttg aaagtcgagg ataataggtt agtctaccac cacaatcaat acaataacga
     5761 ttggcccttc taattctggc caatgtaaac ctcctgcatt attcctaatc tgcgatcaga
     5821 tattagatct cactggtcta agagaataaa cccaaacggt tgcaacgatc aatatttcta
     5881 aattcgaatt tagaaatatc tgaatattaa actgtacaat cagcaaccca taaatccaca
     5941 cctgcaaaat aaatctttca gaattcgaat ttagaaagaa tatgcgccgc ccgtcagact
     6001 tgaactgacg accccctgat taacagtcag gcgctctacc acgctgagct agggcggcaa
     6061 cacgtttgta ccttgatgta tgcgatttaa tccttactag tttagtgatg aaaaattaaa
     6121 attatttttc tattccttta cccttgtaat acaggtaaat gccatcaaga aaaacgcggt
     6181 ggtctttgcc ctttaccttt gtttttgttt ggatttcagc agctgtttga gtcacgtcgt
     6241 ttagtactga acctgtaact atgacatcat ctcctttaga gactactttt gtggaaccgt
     6301 gaattcttgc aactcttggt gctcgctctc cctgaaagtt ctcaattaga actttgtcgt
     6361 ctttggtctt aacagtaatt ggaaagtgcg catatactat cttcattttt accgtatagc
     6421 cttcaaccac cccctcacac aggtttctaa ttatggaaga agcagtgttt gctattgcag
     6481 agtatttctt tctggaattt acggccttta ttgaaacttt gttgtctttg acttctagct
     6541 cgactggaat ctttttgaag tttttgtagg ttttgcctaa cgggccttca acatgtaaca
     6601 tgtgtttttt tagagaaacc ttgactttct gtgggatttc gtatgtagac tcgaagatct
     6661 ctgcttgttt agtagacata tcctatcaaa aatcctccaa tcccattatt ttcagcttca
     6721 tgatgggaca taactccttt atttgtagta acaagtagca tcccacgatt atatgctgga
     6781 aggtattgct gctcccagtc agcatattca ttctttttta ccctaaatct aggcgtaatt
     6841 gaaccacatt ttgtaatgtg cgccaaaagc ttaatcttga atttgcctcc cctgttgtcc
     6901 tcgacgtgtt cgaattctcc aatgtagttg tgtttctgta gcgtttttag aatctctatt
     6961 gccagttttg aggttggcaa gattacgcat tcggttcgcc ttcttgcttc gttgttgtac
     7021 aaggtattga atatgtttga aattatgtta gttgctggca ttttctcacc tgtattttat
     7081 gaatcccaag gaatctgcta cctctctgaa acatcgcctg caaatcataa gattgtattt
     7141 ttgaattact gctgtgtaat ctccacatcg cttgcaccag cgtgagcctt tgccgaattt
     7201 gtgctctttt cttcccgtgt acttgtacga cctatctttc attgtactat cgaaactcca
     7261 aactctctag ataaaaactc agttgcttcc tcttttgaaa ttctgtgctt ctttccgacg
     7321 cttgctttgt gtctacttct cattctgatg ctaaagccag gccttgtcag tgttactgca
     7381 gcttcgaggc ccaatatgcc aatttgtgga tcatatttta ttccaggaat gtcgatgtgt
     7441 tctttgattc caaatgaaac atttccaaag tcatcaaatg atcttgcctt gattttgttt
     7501 cctaccgcat caagtagccg tttaagaatt gttgcagcgt cctgtccccg tacagtaacg
     7561 gctacgccga ttggctctcc cttccttaca ccccaatctc gttgcgttgc tttggcatca
     7621 cgtgcgcatg attttttgcc cgtgattgaa tcaagtgcct tttttgctat cacaatggcg
     7681 tcaccggatt tacccacacc catgtttagg actaattttt caagtctaat tattctcatt
     7741 gggttttctt gattgtctgc catagtatca cgctatttga ataacaggtt cctttttgcc
     7801 cacaaccatc accaagtctg caggtatttc gatttctttt tgctcaagtg agagcaaaac
     7861 acgctttgga agaacaaatg ttccttcttt gatctctttt acttcggcaa cctgccctgc
     7921 gttgattcct tttgtgacaa ttaccttcac accttctttt agatttagta catcattgat
     7981 tttttgttca ggtacttgta tgacgcatga atcgcccaca ctagcatcaa tatctgaaat
     8041 cagtgagcgc ccatcatgga acccaagctg cagcttacct ttttttattg tagtcttgct
     8101 tgttactttg actagtttta ttgatttttc tgagctgttg attttgattg gttctaagag
     8161 atgaccttgt tttggcacta ggcggtaaat gtcagaaacg ccatcaagtt ctatgacatc
     8221 cattaatcct atgccgtgat gaattgactt tcttttaact ccatcaatct ttactttgcc
     8281 attgtaaatt gcgtagtatg cctctcgtgc agtagttaca agctttagtg tgtcacgtaa
     8341 aaatactgcg gttgggatcg aaaagtgttt tgaatgcgga cctggcctaa cagtagttac
     8401 aaatcgtggt ctttttctta aaatgcccca aaacattgga gccatttgtc ttttgagttt
     8461 cttactacct gctattcgtg tcattacttg tcctcctttt tggcgttttt agctgttttt
     8521 gattctttgt tctcttttgg tttttcttgt tttacttctt gttttgcttc tttttgcggg
     8581 gttgtttttg gtttttcttc ttttggtttg gtttcctttg atttttgatg tttgccttca
     8641 agtttatttt gacgccattt gtcttcggtg ttaagatcag ttatcaaaag attagatggg
     8701 tgtatgaaga catcgacgtt tccgcccttg agtttttctc tctttattcc ttcaacggct
     8761 acgccatttt tttctgatga aactttaata atttttccac tcactccttt gtattcccca
     8821 cgcattactc gtaccgtgtc cccctcggtt acgcgaacac ttcgtctgct gtacttgttc
     8881 tgaagatctt ttgtgagatg gctgcaaaga agtctgctct taacagcaag tgatgctttg
     8941 aaaattgttc tgtttcgaat tattgagggt ttcattttat accaccattg atgccaaatt
     9001 tgctaccctt ggccattttt ctgatgcttc tgcggcaaca ggtcctttga tatcagttcc
     9061 ttttatttcg ccttctggcg taacaagaat tcctgcgttg tcctcaaagg ccactcgaac
     9121 cccatttaat cttctaattg gatatttttg tcggattatt actgcaccaa agatttgctt
     9181 tctaagctct gctggccctt tctttaccac aacattgaca aaatcaccta cagccgcgga
     9241 aggcattcgt gatgttcttg tctttgccct ctttaccata attatttcta aaatttttgc
     9301 accggtatta tcagcacatg tgattcttgc gccaagagga agcgccctcg taatgtatgg
     9361 tctgaattct tgaacgccct ttgcagatac tgcacgactt tttcctgtgg acattttacg
     9421 accgtacctc cactactaca aatgctactg actttgcaat aggcctgcat tctgctgcaa
     9481 taacgacgtc gccttctttg agatctaaat tagcagactt gtaagcatgg attgtacttg
     9541 tacttcttgc atatcgtttg gtctttgtga tgtaaagtgg gttttctctt cgaattacca
     9601 ccgtattctt gttctttgcg ctgatcactt taccctcaaa tagtttacct ctgacgctaa
     9661 gcgattttcc gttatccatc acgagtccct tatacacgtg tttttgtgcg tctttactca
     9721 tgctttcacc ttgattctat cttcaggccg ttttgatatg agtgctccgt cgactgtaaa
     9781 gtcctgatca cctagtgtaa atttccattt gttgtgttgt tttgaaacca tttttgttcc
     9841 acgctttgtg tcaagcaata acattgactt tgtttcatca attactttgc ctaataatcc
     9901 aacaactgcc ttatttgttg attctaaaat ttgcgcatct agtcccagta gctcgtgatt
     9961 tatgatgttt tgttgtgtta tcattttctt ttcaactcgt ttaatctagt caacatgcgc
    10021 gctatttctt ttctcaatgc ctttatcttc ccactctctt tacgtaatgt tccttttgca
    10081 gcatcggttc gaagttttgt aagctcggtt cttgattgtc ctagtctttc ttttaggtcc
    10141 atttcattaa attctctaat tgatttcatt tttagtcgag ccattacttt aactcctcct
    10201 cgatttcttc cataagatca agcttttcaa ttctttctgc atctattttg atttgttcgc
    10261 tttctgttct agccttgact tctgcatctt cacgttcttt tgccagcttt cgttcttggg
    10321 cagctagctc ctcttttcct tttagttcaa agtcaggtaa gtatcgctcc tttcttgcga
    10381 ttcgtattct cacaccaatg taacccattg gggttgggac gtgaacattg tcttcatcaa
    10441 ctgcaagcgt tgctttgtgc cctgctcgtg gaagaattcc tgcagaatgt ttttcaaatg
    10501 atgaacggtc acctctcagt tttccggaaa cagtaatttg aacacccaat gcgcctgcat
    10561 tcatgatttg ttgcagtgtc cacattgtag cccttctaaa cgcggttcct cgctcaatta
    10621 atgacgctag tctgttacac atcacacttg cagtaagctc tggttctgtg atctcattta
    10681 ctgcaatttg tggactcttt aatccaaaat ctttttcaag tttttcagtc aactctctaa
    10741 ttcctgctcc ttttcttccg atcactattc ctggtcttgt tacatgtaaa acgactcttg
    10801 ttccaattgg agtctttgag atatcaacat gcgagaaccc agactctttg attgcggttt
    10861 tgagatattc tttgagaagc atcatgttgt agttgtcttt gattacgttt ttgagtgatg
    10921 acaattttac ttctcctgag ccacaagctc aatatgtgtc aagacattgt tttttggagt
    10981 tgctctgccc atcgcacgtg gggtgaatcg ttttatgagt accccgcggt gagtgtttgc
    11041 tccaacaatt tttagcctat caagatccat tcccttgtac tctgcattag cttctagatt
    11101 atccaaaagt ttgatgaatt ccgtggcggc cttacgcggg taacgaccag acattacgcc
    11161 agtatcagat ctgtggccaa cctcattttt gtatctccta aacggaactg cacgctttag
    11221 agatatgact tcttgcagat aatcacgtgc tttttcaatt gaaaggcctt tgatcatttt
    11281 tgcaatctct cgtgcgtgtt tgtgcgagac acttttttct ctgactgcgg ctctgacgtg
    11341 ttttgtcggg tcaaaatttt gaaaagcata accaaattga ggcatagtat attcacttta
    11401 atggcacgta cagacttgat cttgatgcgc cgacaccggg agcgccatga acaactcgtt
    11461 tgtttgttct gacatattca ccggtatagt gtccaatcat ttgtggagtg attgtgaccg
    11521 gcagaaactc tttgcctgaa aatacgttaa ttgttacgcc gaccatgtag ggtaaaacaa
    11581 taagatctct gatgtgtgtc tttatcgggt ttttggactt gcctgccttt tctagtttta
    11641 tttcctctat tagctttcgt tttccatcag tgatgcctct tgtcagtgat cttcgtgccc
    11701 tcgctggaaa tatctcaaat aatttttcta gcgacatgct ttgcagctgc tctagtggta
    11761 ttcccctgta atcaaattcc ttaaccattc tttgacacct ttaggattga gatttgcaag
    11821 tccatattat agcattccta tctttgtggc caagtctctg gctttttcta cggattcgaa
    11881 cttgacaaag gcctttttgc cagatattgt ccttgctgtg tttattttaa ctacgtcttc
    11941 ttcgtaaagc gttttgattg cttcctttat tgttttcttg tttgcttgtg gatcaacaat
    12001 gaagcagatt ctgctgtctt tttctactag cgcaaatgtt ttttctgtaa tgtgtggcct
    12061 gagaattatt tttgtggcct gagttgcatt cattgtattg cctccattaa ttgaagatgt
    12121 ggggatttga tgtttgcaat ctcacctatt gcagcctttg tatacacagt taatctaatt
    12181 ggttgagagc caggagccaa gtcaagaaca gatagatcct ttgcttctct cacatcaact
    12241 cccaaaaagg agccgcatgc ttttgcaaga ctttttgcgt cttttactac aaacagaatg
    12301 ctttttccag tctttgtttt gcgtcctcga attaccgccc tgccagaacg tgcctttctt
    12361 ttctgtaacc tttgtatgtc ttttgccagg tttaacgcat caaaaacttt ggagatttcc
    12421 tttgctttcg atattttttc aacgtcatca gatacaatga ttggaaatga tgtaatgtta
    12481 tcaactatat gtcctcgtga tgctatgatg tcttttgatg tagtagcagc aattgcagaa
    12541 catagtgcga gcctgttttc ttttttgttt agctttttgt aaattacttt ctcagatgtt
    12601 ggtggatgtg cttgtcttcc gcctcttgcc attgcgactc ctgctgcttc acctcgtctt
    12661 ggccctccgc ctcgaatcct tgctattctt gcttggccgt gtccagttgg cggatcattt
    12721 gactttgcaa ctacatccat tcctgctgtc gggtgtctcc cttgtctttg gtaatgatga
    12781 gaatccaagt tcacataggc tttatgaata agatcttttc taaacggtgt tgagaaaacc
    12841 tttggaagat caatttcttc ctgttttgtt ccgcttaatg acagtgtttg agctttcata
    12901 ccacaatctc caatatgtta ggcttgagaa tctttgattg aatgttacgg atttgcgttc
    12961 tgagttttac gagtcgtcgg tatgttccag gaattgatcc tttgagaaca acaaagtcac
    13021 catctacaag accatagtgt ttgtatccgc ctgtaggatt tattttgaat tcattttttt
    13081 cagtgttact cattatcatt attcttttgt tgtattctac gcgttgatga aaaccgcgtt
    13141 gcccacctct aggcacagta tacatgatgc ttgatggcga gattggccct agtgtaccaa
    13201 gtgcccttac actttttctt gacttgtgtt gttttttctt gacaccccat ctggtgatcg
    13261 ggccttccca tcctttgcct tttgttatcg acgcaacatc cacagttgag ccaagctcaa
    13321 atacatcttc gactttgact tgttttccga aaataccttt tagaaaatca aattgttttt
    13381 tgatgtctcc accttttact gcaacttcaa agacgtatgg tttcttttgt gccagtccga
    13441 tgttgtttgg cgatactgca acgactgcaa aaagatcact tattctgtta agaagttttt
    13501 ctgctttttc caagctgcct tcttttggtt ttatagtaat taatcgcgat acttcttttg
    13561 gcatatcatt gctgaaaaca tcaaataatg catgagtgcc gtcaacgtct tcatgataac
    13621 ctcgaatgcc aatcacggca actggtggag ttgcaacaac tgtcccaaga cttacaagct
    13681 gttttccatg gtttggtgtt ttttctctgt catcgatact gacaatttgt atgcagccta
    13741 ctttgaaacc ggcatagcct aacagttttg gttgttctga atttatttcc ggccaagtcc
    13801 taattcgtgc ctccatgctt ttggcacgtc ctctaggcaa atacgcaaga ctgccacgac
    13861 gtggctggct atgttttcta tgacccatag ataatcgaaa aaactcgtta accccattat
    13921 atacctaggg gtaacgaatg ttacggtttt gctatgtttg tgatcgaaaa gaacagtgta
    13981 attccgccga tgatcattcc ggtaattccc accaaaatgg caagcaataa aatgtttttt
    14041 tgtcttggca tgatttcaga acgaatcttg cattaatttg agtttccaaa attcaaaata
    14101 gatagaattc caagcactgc ctcttcgagt cggattgttt cagttgcctg atttgcaaaa
    14161 aagtttagca ctcttgcatt gtgaacttcg tttatttttt tgcccaagat gtcgtacagg
    14221 cctcgatctg gtgcgccaaa tacgatcaaa agtggcttat cagaacttgc atattctcga
    14281 atttgttgtg cggttgcttc ttttccctta cgtgacgtga gaataacatt tccattccat
    14341 tcttcaagaa tacttaccaa gtttggtctt tcctttacct tgtagcccca atattgtttg
    14401 atgtggaatt cagagatctc ttttgctgtc agttctggct ctacggtttt gatttgtact
    14461 gtgattcgtt tcttttcatg ctctttacca aaatatggaa ttaatttgtg gactccaatg
    14521 tcaatgaatt tctttcccct gtagtgtact attgcgccct ctctaatgtc accttctttg
    14581 agatttttcg ggtttgatga taatgagtga tgtggaattt gtagtggatg caatattcca
    14641 gcatatttga gctcatcaat ttttggaaac agtgttcttc tgagatattg cggtgtttct
    14701 aaatatttga ggacagtcga taagagaaag cgatccgagt agtttcctcc actttcctcg
    14761 tagacaaaga ttgtatttac actaaatatt gcgcatgccc tggcaataga tgagacttta
    14821 cgcgatttgt ctagcttagt tgcctcgtcc ttaagagagg aatctgggat tgcgactgag
    14881 atattcaata ttgaaaaatt ctaaacgttt gggattggga atttttgttt gccgattttt
    14941 gcatagtttg caatttgctc tattgttact ttttcaaact gctttgtcga ggttttgatg
    15001 tgagacatct tgatgtgatc ggttccttct ttttggtcgc ttgcaatgta aatgcatgca
    15061 gttgccagtg ctgatgcatc ttcaagtgac atgtcaggtt tgtagttttt ctcaagaaag
    15121 tcatttgctt gttcagagtt tgctccaatt gctacggcgt catatgctat gtaagttcca
    15181 cttgggtctg tcagaaataa cgagttacca gaagaatcta ccccgccaat aattaatgca
    15241 acaccaaatg gtcgcgcacc tccaaactgc gtaaactgtt gacattggtc ggctaggtgt
    15301 tttgccacgg tttccacatc aactgcctca tcatagacaa ttctgttact ttgtgaaaaa
    15361 aatcttgccg tatctgcttg gcttcgtgca tcaggtatgt aacctgctgc tgcaaatcct
    15421 atatgctcat caatttgaaa aattttgtgt gctgattctg aaatttgtaa tttgcgtggt
    15481 ttttcttcta ctgcaatgac aacaccatct ttgcatttga ctccaactgc aattgcacca
    15541 cgtcggaccg tttctattgc atattctact tggtataacc ttccatcagg agaaaatatt
    15601 gtgatggctc tatcataacc ttgttgggaa ggaagcaatt ctattttgaa tgctttgaaa
    15661 gtttaattta agtctagtta tgctgcactt gtgcgatttg agattccgtt ttacggtcac
    15721 aaaaatgtca gagcacttca tccaagaaca atagagatca caaccgagcc tgatctcacg
    15781 ctggatggag actgcataat tggagttagt gcagattgtg ggtgcaagga gatcccaaaa
    15841 aaactaaagc agaaactcag gcaaacagac tctaaaataa caatcacaat tagggtcaag
    15901 ggaaaatcat tcacaatcac aggaaacggt catccggatt tgaggttaga aaatccacat
    15961 gacatagtaa ttagaaaaag caattttttg tgccccagaa ctttggcagt tggttgtgat
    16021 ttggcatcaa atgacatgcc gcgccagatg ataaaattac tacaagatcc aaaaacaaaa
    16081 ggattgtttg taatcgaagt tacttgattt ttttctgaaa tgtaaagtta ttattttcca
    16141 aaacatttgc catcgccctg ttgacaattt ccatcaagac aaattcatta tgaactgatt
    16201 ctgatttttt ttgtagagca ggtttttttg caatcccatc aagtattttt tctatcttag
    16261 ttgacgttga ttttatcatt gaagagtatt tgacatcaaa atcttttgag gacgtatttg
    16321 caattagctt aattgatgtg ccataaaact ttgcaattgc acctctgatt tcttggattt
    16381 tgacaatttc gatcagacca gcttctttta ggatttccag atggtgacgt attgtagtta
    16441 ctgctttatt gcaaccgttt ttttgtagtt cctttgtgat ttggtcagtg gtgagttgtt
    16501 tgtggtacag tatttccacg atttttgatc ttgctgggtc ttcaagtgct cgggcctgct
    16561 caacagaaat cgtcatgact cggttaattt tgatttgttt ttcaagcaaa gtagacatgt
    16621 tatctgcaaa tcatgggttc agctaaaaga tcctagcatc ataatttctt gtccattcgc
    16681 atcatttttt ttatgttcca acaaatttcg tagcaaacga ccaaaactat ctttttgcta
    16741 ctaagaatgt tgttgtgaaa agaaaacttt cgtaccgctc tgaactaatt agtagcaata
    16801 ccaataattt caaaaaaagc aaaattttgg caatgtcggg gagctatgtt gccaaacagg
    16861 tgcagaaccc ccaaagggaa gggtctgaga aattcaaaat tagagttaag ctgaaaaaat
    16921 tgagcctaaa gaggtttgcc agatatgcgc tcataagaga taatgtagcg ctccgacatt
    16981 ttctgaatta tttcttgagg gattgaaggt gccacgggca tttttccttg ttttcttgca
    17041 tcatcaaatt gttgctggta tccgttggca gtaagccagt cacgaagtat ttgcttgtcg
    17101 tatgcctcct gtatttttcc gactgcatag atttcctttg gccacagtct gtactcatca
    17161 ggtccaattg agtcgccaag aattatttgg tcatcaagct tgccgaactc tagcttcaag
    17221 tctgccaaaa tgaaccctgc tgcgtcggca acaccataca tttggttgta aatctcaatt
    17281 gattttaatt caagccaatt gtattctgtt tcggtaacta ggcctttttc tgttgcagtt
    17341 tgtttgtcta ttggttcatc gtgttcagcc tttgtagtag gatcaaaaat cggcactggg
    17401 agttttgcgg caatctctgt ttgtgttccg gcaggcagtg acacttgact tgccttccaa
    17461 cgtgtgatca agcttccata aaaataaccg cgcaccacac attcgactgg aatcattttc
    17521 atttttttta caacgatttc attcttcgac acagtcttta caaaatggtt tttgactgga
    17581 atcttggtaa accagaattc cgcaaatttt gctaaaacct cgcctttgta agggattgga
    17641 tcatggaact tgacatcgta tgcggaaacc ctatcactaa atttgaatag gaggtttccg
    17701 tcctcaagat catagacgtc ctttaccttg cctgaaccaa gaaatttcaa tttataattt
    17761 gcataatgaa ttagccatta taacaatttt tgaatgtgtt tttaatccag tgattattaa
    17821 tgccaacatt gagaattctg cttttggttt taggtttgtc actgggtttt gtgcctgttt
    17881 ttgcagatga acctcaaatc aaggccgatg atctggtagt cgaaaccttt gttgataaca
    17941 ttccaaataa tccaaccaca atgacgtttg ttggagacga tattttggtg ctccaacgat
    18001 ataacggcca agtacgactg gtccatgatg gcgtcttaca agacaaacca gtgttagatg
    18061 tatcagttgc aaaagacggg gaaagaggaa tgcttggaat cacatctatt ggctctacag
    18121 tctatgttta ctttactgcg gccgacacag acgggggaaa agcaattgag aatagaattt
    18181 tcaagtatga gtggaatggt agtgagcttg tcaacccaat tctgctaaag cagttgccag
    18241 gtgacaacta ttatcacaat ggtggcgcca tgacaaattt tgatggccaa gtttacgcag
    18301 taattggcga caatggcaat tatggaaagc tacagaacag acaatccaat tggaagaacg
    18361 atactagcgt catactgaga gttgatccgc caggaccata ctacgcaatg ggaatacgga
    18421 atagttttgg aatcacattt gacccattta ctggaaacat gtgggatact gaaaatggtg
    18481 atgatgattt tgatgagatc aatttggttc cagagagttt caatagcggt tggattgaga
    18541 taatgggtcc tgcaaaaaac caatcacaga ttgactcttt gccaaagtat ggaaactttg
    18601 tctacagtga cccagagttt agttggcaaa aacctgttgc accaactggg atttcttttg
    18661 taaagtctga aaagatgtta gattaccaag atagtgtctt tgttggagat tgtaatacag
    18721 ggaatttgta cagattcaag ttaaattctg atagaacgga ttttgtattt gaaacccctg
    18781 aattatctga caaggttcta aatcttagtg acccaagtga tgagatcatt tttgggagtg
    18841 gttttggctg tgtaacagat attgagctag ggcctgatgg attgctatac atcgtttccc
    18901 tttctgagga aaaaatctac agacttgtgc caaaatctat ggctcaagct agccaagagc
    18961 cagagatagt tgatggtggt tgtcttattg caactgctgc gtatggaaca gagttagcta
    19021 ctcaggtaca acttctcaga gaggtaagag acaatgttgt gtttagcaca cattctggca
    19081 cgacattcat gacgggattc aatcaggcct attattcatt tagcccgacc atatcggact
    19141 gggaaagaca gagtcctgta ttcagagaaa tgatcaaaat cataattacg ccgatgctct
    19201 caacgctatc attactcaat tatgccgaaa tcgattcaga gcaggagatg cttgggtatg
    19261 ggattggaat cattcttctt aatgtaggaa tatattttat tgctccagtc attctgattg
    19321 aaaaattaag gaaactaata gataaatgta atagaaaatc ataatctaaa attttacttt
    19381 atagtatcat aattagaaag agaaaactag gaaacaataa tatgcgttat tacttttcgc
    19441 gttttgtttg acgcatcaag gactgacacc agcttcaatt aataaaagga tgatttttgc
    19501 tgggattttt ttgatctcct tggcaggtct cgtactggag atcgccatta caagaatttt
    19561 ttcagctgct gtttcatatc attttgcctt tgttgcagtg tcagttgcac tcgttggcct
    19621 tggttcatct ggacttttgg ttcactaccg attaaagaaa atcaaagaaa actgggcctc
    19681 cgatctcgca atattgtcat cccttggtgt aacgctagta attccattga ctctttttgt
    19741 aatgcattac tttgcctcac gtgtaatgta cctgcctttg tacatgctcc tctttgcagt
    19801 ctctttcttt tttattggca ctgttatctc agctgcattt agcgcctttg ctcatgttgc
    19861 tggaagattg tatgcggcag atttgattgg tgcgtcacta ggcgcagttt cagtcgtttt
    19921 gttattatcg atgttaggag gagaaggaac tgtcctaact gttggcttca tctctgctat
    19981 ctgtgccaca gttttctcaa ttgtagcaaa aaacaaaaag cgggttttga tcagcctagc
    20041 atttgttgcc tttgcagcag ggttgttgtt catcaacgaa acaatagaaa tattttcaat
    20101 tcaaactaac cccgatgcac aaaaagacct gccattattt ttgaaagaaa atcctggcgc
    20161 ccacattgta aagacgcaat ggaactcttt ttcaagaatt gatgtagtcg aggggattac
    20221 tgggaattgt gctggtagtt ctgatcctta tgctcaaaga cagtgttctc aaaaagacat
    20281 tgttgcaaaa attttcattg atggtggagc tggaacaaac ataattttct gggatggaaa
    20341 tccagatagc aggcagtcgt tgtcttcttg gatgcaatac gtaccgttta agatgaaaga
    20401 tgaccccaaa gttcttgtta ttggttctgg tgggggaaga gatgttgtag cagcattagc
    20461 cagtggcagc agagacgtta cttctgttga gataaaccca atcatttttg aaactgtaaa
    20521 aagttacggg cagaaagctg gtaacctgta tactcaccca tatgtacact cccatataga
    20581 cgagggtagg agttttattt caaagtctca ggaaaaattt gacataattt acataccttt
    20641 tgtagataca tgggcttcag tttcatctgg gagccttggg gtttcagaaa actttcttta
    20701 caccatagaa ggttttcaag aatattatga tcatttggaa gacaatggca aaataattgc
    20761 ggttagatgg ctcatagatt cacccagatt tgtctcaaca tttgccgaac tgcttgaaca
    20821 aaatggactc tcacgtactg agcttcaaaa acatctcgta attgtaactg ccgaatcgac
    20881 agaaaaagat ccaagcgtga ctatggtaat attttcaaag tcaccattca ctgaaaacga
    20941 ggtacaattc ctgtcagatt cttttgaaag aaacggatac aaaccgatac tcatgccaga
    21001 caaaattgca ccagagaagt attctggttt tttgagtgac aaattatctc atcaggagtt
    21061 ttatgctttg tt
//
LOCUS       DASWKH010000114         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1791101, whole genome shotgun sequence.
ACCESSION   DASWKH010000114 DASWKH010000000
VERSION     DASWKH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791101"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VGA92_08645"
     CDS             complement(<1..799)
                     /locus_tag="VGA92_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9846509.1"
                     /translation="MQNYLLQVGQQSASTINGEVKNIIDQITPEIPHSSFVTDLAIIM
                     ILASVVTLAFFKMRQPLIIGYLFAGMLIGPLSPIWSSVLPEDNGFMGLGKTGILSDIA
                     VLNIFAELGVILLLFVIGIEFPFSKIRSIGKIAVGAGTIGLFMTLGIGFYVSSLVGLN
                     FMDSLFLSAALSISSTAILVKILEETGKIKKESSILVLGILIVEDIIAVILIATLESV
                     ALVGSVSIENIVVVAVVAGALIAGTLTVGIRVIPRLIDRVAATEHKEI"
     gene            complement(840..>2436)
                     /locus_tag="VGA92_08650"
     CDS             complement(840..>2436)
                     /locus_tag="VGA92_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9846510.1"
                     /translation="PYGLKTNPYPSSPTPTEHDAKILGGSRHQEAKASILECISDLYK
                     KVARRNSTDNDFRMVTIVQDVGSGKTHLALHIKTLKTRQDIVTTFVDLSTISPKTNDS
                     IYNAIIRGFNKELFSELKEKFLLQMCEKAQKGDVLAKKTLGYGLVDKLKGITLKQKTE
                     DIMYDRKPVSAEALQRYLAANFNQHEAVIIRNVISNSFDKITNLDDLHGRLGAIGKFS
                     HNLLGKVILFELDELDAQEGSLDFVKSLINAHIPASILLLITTPSLYMDVQRANPSVF
                     DRLEKANYKIDLAGANSIDELIEIAIEYIKEADKTERFNKKEQQDLAAKIRVLCDEFP
                     EFRNVRSIINILNHAIEKAGELEASEITEAVIDETIKQTYPGLKIKGSIMEVPISEFI
                     KIKRIVGTNQNQVKQAVSNLVNYAHEMGNISEANKSNPSFDVVYNDAFGTKVGIIVVM
                     DPSHSKNFETITNVVKSSAFVDKLVILTNTTIPPSNQATIVNVDKSKVMDLLYFNTKY
                     IEHKLEEPESEKIKMLAKTICVI"
BASE COUNT          621 a          509 c          503 g          803 t
ORIGIN      
        1 ggatttcttt gtgctcagtt gctgcaaccc tgtcaattag tcttggaatt accctgattc
       61 ccacagttag tgttccagca atgagtgctc ctgcaacaac tgcaaccaca actatgtttt
      121 caatggaaac agagcctact agtgccaccg actctagtgt tgcaataaga atgacggcga
      181 tgatgtcctc tacaatcaat atgcccaaaa caagaatgga tgattctttt ttgatcttcc
      241 ccgtttcttc tagaattttt actaggatgg cagtactact aatggaaagt gcagcactca
      301 aaaacaacga atccataaag tttagcccga caagacttga aacgtagaat cctattccaa
      361 gcgtcataaa caatccgatt gttccagcac cgactgcaat cttgccaata gaacggattt
      421 tggaaaacgg aaactctatt ccaatcacaa acaaaagtaa gatgacgcca agctctgcaa
      481 agatgttaag caccgcaatg tcagaaagta ttccggtctt tccaagtccc ataaagccat
      541 tgtcttctgg cagaactgag ctccagatgg gcgaaagcgg cccaattagc attccagcaa
      601 aaagatatcc tatgattaag ggctggcgca ttttgaaaaa tgctagtgtt acaacagagg
      661 ccaaaatcat aattattgcc aagtcagtga caaaggaaga gtgtggaatc tcaggcgtta
      721 tctggtctat aatgtttttg acttcgccgt taattgtgct tgcagactgt tggccaacct
      781 gtaaaagata attttgcact agattttgat aaacaaaatc attaaaatga gtttggtttt
      841 tagattacgc agattgtttt tgcaagcatc ttgatttttt cgctttctgg ctcttctagc
      901 ttgtgctcta tgtatttggt gttaaagtac agcaaatcca ttacctttga tttgtccaca
      961 tttacaatcg ttgcctgatt tgacggtggt atggttgtgt ttgttaggat tacaagcttg
     1021 tccacaaatg ccgatgattt gactacgttt gttattgtct caaagttctt tgagtggcta
     1081 gggtccatga ctactatgat tccaaccttt gtgccaaatg cgtcattata caccacatca
     1141 aatgacgggt ttgacttgtt tgcctcagat atgtttccca tctcatgtgc atagttgacg
     1201 aggtttgaga ctgcctgctt tacctggttt tggtttgtgc caacaatcct ttttattttg
     1261 ataaactctg atattggcac ttccataatg ctgcccttta tctttagtcc cggataggtc
     1321 tgctttattg tctcatctat tacggcctcg gtgatttcag aagcttcaag ctcgcctgcc
     1381 ttttctattg catggtttag aatgttgata attgagcgaa cgtttctaaa ttccggaaac
     1441 tcgtcacaca gaacgcgaat ttttgcagcc aagtcttgct gctctttttt gttaaagcgc
     1501 tctgtcttgt ctgcctcctt aatgtattca attgcaattt caatgagctc atctatggag
     1561 ttggcacctg caagatcaat tttgtagttt gccttttcca gcctatcaaa tacagatggg
     1621 ttggcccttt gaacatccat gtataatgat ggtgtagtaa ttagcaaaag aatcgatgcc
     1681 ggaatgtgcg cgttgattag agactttaca aagtccagtg agccttcctg tgcgtcaagc
     1741 tcatctagct caaagagtat tactttgcca agcaggttgt ggctgaattt gccaattgcc
     1801 ccgagccttc cgtgaaggtc atcaagattt gttattttat caaacgagtt ggaaattaca
     1861 tttcgtatta tgactgcctc gtgctggttg aaattggcag caagatatcg ctgtagcgcc
     1921 tctgccgaga cgggttttct gtcatacatg atgtcctctg ttttctgttt tagcgtgatt
     1981 cctttgagct tatcaactag gccgtatcct agcgtctttt ttgccaagac gtcccctttt
     2041 tgcgcttttt cacacatttg caagaggaat ttttctttga gctcagaaaa cagctccttg
     2101 ttaaagcccc gaatgattgc gttgtaaatg ctgtcatttg tttttggcga aattgtagaa
     2161 agatccacaa aagtagtcac aatgtcctgt cttgtcttta gtgtcttgat gtgtagtgca
     2221 aggtgggtct tgccagaacc aacgtcctga actattgtca ccatcctaaa gtcattatca
     2281 gtagagtttc ttcttgccac ttttttgtac aggtcagata tgcactcaag tattgaggcc
     2341 tttgcctctt ggtgtcttga tccaccaagt atttttgcat cgtgttctgt aggggtggga
     2401 ctgcttgggt acggatttgt tttcagaccg tatgga
//
LOCUS       DASWKH010000115         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_291882, whole genome shotgun sequence.
ACCESSION   DASWKH010000115 DASWKH010000000
VERSION     DASWKH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291882"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1666
                     /gene="argS"
                     /locus_tag="VGA92_08655"
     CDS             60..>1666
                     /gene="argS"
                     /locus_tag="VGA92_08655"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9846511.1"
                     /translation="MSFRILLEEINTNLTNIIHDLGIHETEFVLEPAKEGFGDVTCNI
                     AFLAAKQLKTKPYEIAQSIAKKYEQFLGKLVAKVEAHQSGYLNFYADAAQLCRIIISA
                     TQKGEYGAVDVGKKSHLVVEHTSVNPNKALHIGHVRNVIIGDSVARILSKVNYDVSVL
                     NYVDDSGLQVADIIVGFKHLGFSENPPTGQKFDHYCGDAVYVKTTEKYSEDSHLEQIR
                     NNILKDLEDGNSEIAKFAKTITCKVLAEQLKTCWRLGAYYDCLNFESEIVRSNLWPLI
                     FEKLKEMHLIKYEEEGKNKGCWVIISDEEDKVLVRSNGTATYIAKDIPYAAWKLGLVK
                     DPFHYKKYTVQENGKILWQTTLEQSNEPQQKFAADKVITVIDSRQARLQNIITKLISK
                     FATNSKSYNHLRYESVTLSSDTAGALGMDTRGKNTQMSGRKGLYVNADAILDMLEAKT
                     LGETKKRNPDFSEDQLLKIANKVAVATLRYEMIKQDLDKIITFDHTKSLSLEGDTASY
                     IQYAYARAMRIIEKSNMTPDFDARYELL"
BASE COUNT          598 a          312 c          362 g          394 t
ORIGIN      
        1 ggtgatggtg taatggaagc attggacaaa attgcgccaa ttctttcgga gttgtaagga
       61 tgagctttag aattttacta gaggagataa acacaaacct cacaaacatc atacatgacc
      121 ttggcatcca cgagactgaa tttgttttag agccagcaaa agagggattt ggcgacgtca
      181 catgtaatat tgcgtttttg gcagcaaaac aactaaagac aaagccatat gagatcgcgc
      241 aatctattgc caaaaaatat gaacaattcc taggaaaact agttgcaaaa gttgaggcac
      301 atcaatcggg ttatctgaat ttttatgcag acgccgcgca actttgtcgc ataataatat
      361 ctgcaacaca aaaaggtgaa tacggtgcag ttgatgtggg caaaaaatct caccttgttg
      421 tggagcacac aagtgttaat cccaacaaag cactacacat tggacacgtc agaaacgtga
      481 taattgggga cagcgtggcg cgaattctat caaaggtaaa ctatgatgtg agtgtgctca
      541 attatgtcga tgattctgga cttcaggttg ctgacattat tgttggattc aaacatcttg
      601 gattttctga aaacccgcca actggacaaa aatttgatca ttattgtggg gatgcagtgt
      661 atgttaaaac cacagaaaaa tactctgagg attcacacct agagcagatc agaaacaata
      721 ttctaaaaga tctagaggac ggtaactctg aaatcgcaaa atttgcaaaa acaataacat
      781 gcaaagtact ggcagaacag ctaaaaacat gctggaggct tggcgcatac tacgactgtc
      841 ttaattttga gtcagaaatt gttcgctcaa atctttggcc actgatattt gaaaagctaa
      901 aggagatgca tcttatcaaa tacgaagagg agggaaagaa caaaggctgt tgggtgataa
      961 tatctgacga agaagacaag gttcttgtta ggagcaacgg tactgcaaca tacattgcaa
     1021 aagatattcc atatgcggcg tggaagcttg gtcttgtaaa agatccattc cattacaaaa
     1081 agtacacagt tcaagaaaat gggaaaattt tgtggcaaac aacacttgag caaagcaatg
     1141 aaccacaaca aaaatttgct gcagacaagg taattacagt aattgattca agacaagcga
     1201 ggcttcaaaa cataataacg aaactgatct caaaatttgc caccaattca aaatcataca
     1261 accacctaag gtacgaatcc gtaacattaa gctcggatac tgccggtgca cttggaatgg
     1321 acacgagggg gaaaaacaca caaatgtctg gcagaaaggg gctctacgtc aatgccgacg
     1381 ccatattgga tatgcttgaa gcaaaaacac tgggggaaac aaaaaagcgg aatcctgact
     1441 tctcagagga tcaactgcta aagattgcaa acaaggtggc agtggccaca ctcagatatg
     1501 agatgataaa acaagatctg gacaaaataa tcacatttga tcacaccaag tcgcttagcc
     1561 tggaggggga tacggcgtcg tacatccaat atgcttatgc aagggcaatg cgaatcatag
     1621 aaaaatcaaa tatgacacct gactttgatg caagatacga actact
//
LOCUS       DASWKH010000116         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_666924, whole genome shotgun sequence.
ACCESSION   DASWKH010000116 DASWKH010000000
VERSION     DASWKH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666924"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..277)
                     /locus_tag="VGA92_08660"
     CDS             complement(2..277)
                     /locus_tag="VGA92_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846512.1"
                     /translation="MNYQYPLLGGIGVVAVLVIMYAAVVMTVPHQTAIDSVENTGVES
                     SMTSQEQSLSQIQITDGIKHIVPLDKIKSGGPPKEIWDKILEKSQAS"
     gene            388..>1520
                     /locus_tag="VGA92_08665"
     CDS             388..>1520
                     /locus_tag="VGA92_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846513.1"
                     /translation="MNKILKYAGLLAILPLFTASLTTIAFENPSAEGQRICGDKICTS
                     QNGPDAEKEREKMRQEMAGKTTTSETSQKEETKTFEESDLGSVLRLSRANVPATIPLH
                     KGFYNGEAVYFIITDSSDKTHADTITKNQGWKVELAPPLANAPKEALSKTYMFTNGVN
                     GNGVHGFQGEVFTSTPAQPDKYSALTSHVHVTWNEDVTPRVLDSEQAILDAQAAGDVS
                     LQDLKVVINMPQIVWPGGQMMVKEDKTLSDETPYGGGQVLDIDLDKMTVTFIAHRGWG
                     PDGRTIYYIVTDATPEMPANMMGVVSSPTAAKLIANSAAVDLFQFMNGITGSGPMGFQ
                     AGIAASAPGDQNYSPMWRISVIGWKDPTSAKLLETTTDISAFT"
BASE COUNT          510 a          289 c          317 g          404 t
ORIGIN      
        1 cttaggaagc ttgagatttc tctagtattt tgtcccagat ttcttttggc ggcccgccac
       61 ttttgatctt atcgagtgga acaatgtgtt tgattccgtc tgtgatctga atttgcgata
      121 aactttgttc ctgagatgtc atagatgatt caacacctgt gttttcaaca gaatcaattg
      181 cagtttgatg tggtacagtc atcactacag ctgcatacat tattactaaa acagcgacta
      241 cgcctatacc tcctaaaagt ggatattgat aattcatttt gcaaaaaaaa cggttaagaa
      301 agtattaaaa aattgttcca aatgctcttg cacttttgga tctaatttgg aataatgctt
      361 ttaattgatt agaaaaacga gcatgcaatg aataagatac taaaatacgc aggattgtta
      421 gcaattctac cgctatttac ggcttcatta accaccatag ctttcgaaaa cccttcagct
      481 gaaggacaaa gaatttgtgg agataaaatt tgtacttcgc aaaatggtcc tgatgctgaa
      541 aaagaacgcg aaaaaatgcg acaagagatg gctggaaaaa caactacatc ggaaacatcc
      601 caaaaggaag aaactaaaac atttgaggaa agtgatcttg gttctgtact gagattatca
      661 agagcaaatg ttcctgcaac gattccgttg cacaagggat tctacaacgg tgaggcagtt
      721 tatttcataa tcacagattc aagtgataaa acacatgcag atacaataac aaagaatcaa
      781 ggatggaaag tcgaacttgc accaccactt gcaaatgcac caaaagaagc actgtcaaaa
      841 acatacatgt ttactaatgg tgtaaatgga aacggtgtcc atggatttca aggtgaagtt
      901 tttacgagta caccagctca accagacaaa tacagtgcat taacatcaca tgtacatgta
      961 acatggaacg aggatgtaac gcctagagta ttggattctg aacaagctat acttgatgca
     1021 caagctgcag gtgatgtttc gctacaagat ctcaaagttg taatcaacat gccacaaatt
     1081 gtctggccag gtggtcagat gatggtaaaa gaagacaaga cacttagtga cgagacacca
     1141 tatggtggag gtcaggtgct tgacattgat cttgacaaaa tgacagtgac ttttattgca
     1201 caccgtggtt ggggtcctga cggtaggaca atttactaca ttgttacaga tgcaacacct
     1261 gagatgccag caaacatgat gggagttgta tcatcgccaa ctgcagcaaa actgattgca
     1321 aactcggcag cagttgatct attccagttt atgaacggaa tcacaggatc aggtcctatg
     1381 ggattccagg caggaatcgc agcatctgca ccaggtgatc aaaactatag tccaatgtgg
     1441 agaatctctg tgattggctg gaaagaccca acaagtgcaa aacttttgga aaccacgact
     1501 gatattagtg ccttcacaaa
//
LOCUS       DASWKH010000117        44447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1404926, whole genome shotgun sequence.
ACCESSION   DASWKH010000117 DASWKH010000000
VERSION     DASWKH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 44447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44447
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404926"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VGA92_08670"
     CDS             <1..467
                     /locus_tag="VGA92_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846514.1"
                     /translation="GGGVQSGVTNAFRDLQATVKSGSVTKSLDILSDVRAGHYYSKII
                     PTKTGSLAVELKGTINGVPVNEEVAIEDVESIDILAFPPTGSGGSNQDVLALTNAMSS
                     LQKDVTEIKSKMGNIADGTNIDLSKSYDFAVFGMALGAAGVILAVTAMIKRK"
     gene            complement(477..1352)
                     /locus_tag="VGA92_08675"
     CDS             complement(477..1352)
                     /locus_tag="VGA92_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9846515.1"
                     /translation="MKTIAIGSIFAIFALMVVAPSAFADHAEQVIHIVDQGTGSACGE
                     DQCYVPAEITVDVGTEVTWINDDDAVGSPHTVTSGSAETGGPQGVFPFDLDLISGVGQ
                     EVTFKFETEGEYPYYCMVHAWMKGTVIVQAAHAEDDGEMMDGDAAHVTEMTMDGSVMI
                     TVEAGAPMEGEEMALTVGFTDAGGTAIEHVNFDISATQDGTEVLAEMGQHVHSGSADF
                     TTSALSSDSAVDVQITMLGIGLPDDEANWSGPIGETISLNVVPEFGPIAMIVLAAAIV
                     SIVAVTARSKAILKL"
     gene            complement(1413..2177)
                     /locus_tag="VGA92_08680"
     CDS             complement(1413..2177)
                     /locus_tag="VGA92_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEX9846516.1"
                     /translation="MSPVFAESQRLPTDKGTLLVDITTEPAKPVIGDLTKLKINFINP
                     KTNGIQEHVDYKVTVTKDGVAVFGPIPLQHTGTGSITIPVEFKEKGEHKITVDVELIW
                     FQPIPPETVTFTTMIEAAAQENGDKTPNGNGCLIATAAFGSELAPQVQLLREVRDDVV
                     FSTSTGTAFMTAFNNIYYSFSPTVADWERQNPAFKHVVRATITPMLSTLSILNYVDID
                     SEQELLGYGIGVILLNVGMYFVIPALVIAKLRKFRK"
     gene            2319..3122
                     /locus_tag="VGA92_08685"
     CDS             2319..3122
                     /locus_tag="VGA92_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846517.1"
                     /translation="MTKICIILSIFCTFVLFTSYTNSAAAQETNSTSKSQIQDIVYTA
                     KFVCGSISDDDGPIRPGHYDTSINILNKKGYRVGILLTAVINDGPSSIAIFRNLEPET
                     STGMSCKHIKDMFGIDTKEIVEGFAIIKVPVGSLRGFTNEQTIPDFSEDGINILDVQV
                     FYTANALSTLPHEIAREKIAFYIIQDGTGKIPKESFRKLLDVTIPTTFNEISDTEQKV
                     KSILATKYDLDKNELEKLRIRIKSISIGVGALLDDHAVSLHVVRPQLSS"
     gene            3177..4796
                     /locus_tag="VGA92_08690"
     CDS             3177..4796
                     /locus_tag="VGA92_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9846518.1"
                     /translation="MVSFKKIFVAIFAILLSAQAFTIAQFAYGDGLTQENLPPASFGD
                     RQAALFIKISPPILTKDTVGDTYLQLRLYDANTNQPVPHTSYFISAWKDDKLLLRNLF
                     HAHSGELTLKIVPKNVDVNDVIVYGDQVPQIPGAWTGYNDRVDVHAPVLLESGLYHFE
                     IKIFGIDYDQNIFAEQDVKTFNSWLSVGDISNHTIKYGAESYPVSIVSYYDRIDNFKF
                     DETKKSISFSMPFNWDTTRIEGQTIFVHQEVRVPQAFKEFADTTAYDGKVNGKPIFGR
                     MLILDPYSIEGTSILHFLITKADILDLAKTIPLSTKTMDFTVSPASAVAKNSFTVNLD
                     SGATATVSYDANLGASDTIPLQITFFDENGQILKFVRHGFRIDDSSGKILVENVGGEP
                     QKPGILSIEGIDIQEIKFPAQGNYKMTLVVFNHGLDELTTYQGIGSATFAIGAGGQNA
                     PDYQIPSWIKNNAKWWAEGAITDNDFVQGIQYLIKQQIIKMPSTQSGTGSQVIPSWIK
                     NDAKLWAEGTIGDSDFVQGIQYLIKQGIIKI"
     gene            4886..5659
                     /locus_tag="VGA92_08695"
     CDS             4886..5659
                     /locus_tag="VGA92_08695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9846519.1"
                     /translation="MLPIKDENPKPPGFKPKMTIALIIVNVIVFFFEVAVTGQFFEFS
                     NNNAASLFYNWGAVPACITGERSISIQQFQVSCPAMPMLGLLSSTFLHGGLMHLGGNM
                     LFLWIFGDNIELKFGKLKFLGIYLFWGIIAGLAHVVGDMNSPIPAVGASGAISGVLGA
                     YLVMFPRARVMTFLMMGFFWRMIHIPAKWFLPFWLIFQNIIPFLIGGFGFGAGGVAYL
                     AHIGGFVIGLATGYLYKKTHQSEFMYGSRYGSRYGWKGD"
     gene            5701..5877
                     /locus_tag="VGA92_08700"
     CDS             5701..5877
                     /locus_tag="VGA92_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEX9846520.1"
                     /translation="MPLITVSLYPGRTQQQKEEFAKAITKSAVEILKTKEAHVIVVFE
                     ENPKENWYLAGNSL"
     gene            5919..6725
                     /locus_tag="VGA92_08705"
     CDS             5919..6725
                     /locus_tag="VGA92_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9846521.1"
                     /translation="MTKVAVVQFCASTQKDVNLEKILGFIRKAAERGAHLCAFPEFMM
                     FYTNSKQTPKELATLAETINGNFVTTIAKAAKKNSIQVIGTLYEKSKKKDRVYDTSFI
                     VDKSGKVVSTYRKIHLYDALGFKESAKLEAGSKISGPVKTSLGKTGMLICYDLRFPEV
                     SRILASAGSEILVAPSAWVKGNKKEEHWITINKTRAIENGCYVVAPDQVGNIYCGRSL
                     VVDPYGEILLDMKKKQGIGIVDISLDKVKQTRKVLPLLQNRRTDVYSLRP"
     gene            7217..8371
                     /locus_tag="VGA92_08710"
     CDS             7217..8371
                     /locus_tag="VGA92_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX9846522.1"
                     /translation="MEKVCVLGAGSTKYGKLEDSITDITIQASVGAIESAGIESKEIQ
                     AGYISNVFGIADKQVHLGPVIMSNLGISERPSLSIESACGSGSVSFREAFANVAAGFY
                     DAVLVAGVEKVTHTGTEWTTTYFSYCSDFFYEGGAGASFPGLFASMARAYLTEFKATE
                     EDLALVAVKNHQNGFLNPKAHLRKKITVDDVMKSAVVASPLKLYDCCPFSDGASAVIL
                     CSEKFAKAHSKNYIEVIGSGRGGSPATLQGRDQMTTIPSTKIAAEAAYKMAGVTPKDI
                     DFAEVHDCFTIAEIVDTEDLGFYAKGHGVEAVREGRTALNGEISINPSGGLKAKGHPI
                     GATGVGQVVEVFEQLTGKAGERTVRDARIGLTHNFGATGASCAVHIFKSV"
     gene            8375..8740
                     /locus_tag="VGA92_08715"
     CDS             8375..8740
                     /locus_tag="VGA92_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9846523.1"
                     /translation="MAMKQEFIDTIKSGKILVKKCTKCESLHLATVYFCQKCGNKGFE
                     NKILEGTGTVATYTIITVSPAGFEQYTPYAWVVMKIDGYDLRISGFLGGISSPAQLPI
                     GSKVKIVGYDDRGIILEKQ"
     gene            8812..8973
                     /locus_tag="VGA92_08720"
     CDS             8812..8973
                     /locus_tag="VGA92_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846524.1"
                     /translation="MSVDVICGKCGAKISNMKMLKSIKDVMKHYNNKCPSCGQTLSTS
                     EFSVEIEKN"
     gene            9036..11138
                     /gene="nrdD"
                     /locus_tag="VGA92_08725"
     CDS             9036..11138
                     /gene="nrdD"
                     /locus_tag="VGA92_08725"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEX9846525.1"
                     /translation="MSIEVATGSLDPKKSGGILQSTSKRVRMIFSVMASPNRIDILRI
                     LNSKGPLTYSELKSLAGFKSKKESGKFAYHLRKLLRQSLVALNKSERRYTITNLGKLV
                     LSLARQIEERSIIESGKMYVRTSHESIEEFNSHKIIQSLVREGSLPLELAQKITEEVE
                     NRIYKYQTTYLTGSLIREMVNSVLLEHGHEEYRNKLARLGMPVFDVQEMLTNVDNIDN
                     GAQGLFFKAGQTVFAENLLLNMLPKDVADSHLSGDIHISNPGIWSLLPDTIFFNVKEV
                     IEDGIDLKGKYLGVSRIPTIKTIDNLMASLSMIISLASKEASKEIIMDGLVQLCSKHA
                     KNITELEEKLVGAFTTSSVLSKYTKEPTLVSFRIQLGSEPKIVQAILGAYKNYVKMTP
                     VPQIGLIIDHAVGKISDVSGVVAEIISLGGKVLFTKDETSYSGVVRIKGKNSTSSINL
                     QSLTINLPRLAFESNKDETYFRARLALLMKPALAAMSLRKKDISDLTRRGLNPVLAAN
                     TQYMQRSSVCLVLNLIGLKEAVFNILGYNNDKEGQEIIYKVIQTAVDVAEKKGKEMGD
                     AVIVAMTESDGTSRFGTLDGEKYGKMSVLRSLDGATYSEGVVLASLELDSMNAKAEKI
                     VEANKTSKLLNGGLLVKLQFEKESKVEDIKKALEKAGDLVGAFRPSKEVPICGNCGFK
                     DEKLFEKCPICKSPYVIS"
     gene            11350..14001
                     /locus_tag="VGA92_08730"
     CDS             11350..14001
                     /locus_tag="VGA92_08730"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX9846526.1"
                     /translation="MTTDFSQIESSIVDVKKRDGRITNFNKEKITNAIYKALIANGNP
                     DRKIAEDLTSGVLTKLIEQGFSVGSPPSVEDVQDMVESTLIEKGLGDIAKSYILYRHE
                     RRKLREDKMKVLNTKSLDSVAKTFDLNCLRVLASRYLLRNNKNEITESPSQMFERVAI
                     LVGIGDLLYDAELYSKDASAHQDIEEARRYLTKLDDFEYKFKIGSYYLNKYQFRGLIN
                     HYIYLAKRGQMKKSFKEVLTLIAAKKFDNYAERIQEYYDLMTKQDFLPNSPTMMNAGA
                     RLGQLSACFVLGMPDDMNDIMKSTSDAALIFKSGGGVGINYSDLRHEGDIVASTSGVA
                     SGPVSFMNIINTVTEVVKQGGKRRGANMGILESWHPDIEKFITNKTQAGILENFNVSV
                     GIWEDFWSALVDSQDGKYTLRSPRDKTPVREVNAHQLIDLIAMSAWRSAEPGLIFFDN
                     INKYNVFAKARGMPLRATNPCGEQSLYPYESCNLGSINLANLVKRKADGQYEFDWQRY
                     EETIRKTTRFLDNVIDMNNYPVPEIDQASKESRRIGLGVMGVADLLYKLRIPYNSKEG
                     YEFQSKLAEALTYYSMEESVALAHSRGEFDLCSKTEYPEGKIPVAGYYEKPIGEHYYD
                     WDALISKIQKHGIRNVLATTVAPTGTLSMIADCSNGMEPTFALVFEKRVTVGRFFYTN
                     KIFENVLRENGLYSDELLAKIADNYGSVRGLAEIPEWIQNIFVTAMDIHWSDHLMAQA
                     VWQEWIGNAIAKTINMPHDVSSDDVKAAYLLAHELGLKGITVYRDGSRHTQVLHMMSE
                     NAVKTFDVLPSEYLHEYIGKHIKNTYIRAQVNNALELKIPEEPLKESALEEIREDMLC
                     PSCKNNLVFVEGCSICIECGFSGCTSG"
     gene            14059..15159
                     /locus_tag="VGA92_08735"
     CDS             14059..15159
                     /locus_tag="VGA92_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846527.1"
                     /translation="MNKKIFAAIGIALAVAIGILAVLPGSPPQDLPVAKNEKLGLIIN
                     TPGSAVTLDQLTRVYSEASAAGIGRNNLYLFWNNIEPQPDQYNWRDTDILMSLNKNNN
                     LEVTLYFSIVNGRIIGPYPDWMGAPGFGTILEQNAVKTLDAIISRYGIIDYVIIGGNL
                     DSYFDDTEGSVGLYEEFFTNVYTELKQKHPQVKIGNAFSLNNVLDKNLEHYVTDLSQI
                     GNFVAFTYMPVDRLNDITKTPQEAQEDLQKMFELVPDKKIAVFEISWSTSDLVNGNEA
                     AQAEFIKTAYEFYRQNESKIEFFTWYRQYDRPEGSCAIEQNFPESSVTIAGDQYVRER
                     LASYTCDAGLIKIDGSHKSGWSELKRQIQSSR"
     gene            15169..15837
                     /locus_tag="VGA92_08740"
     CDS             15169..15837
                     /locus_tag="VGA92_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis protein"
                     /protein_id="HEX9846528.1"
                     /translation="MLSLIIAESSLELVPRELQRHNAVTSYCRKMGKRPSEVLLDNSW
                     HFGAMKGIENEIKRGRPDIVHFCLLEACTIPLYEEDEITVYVHTINDQVIQVGKKVRL
                     PKSYHRFAGIIEKLFLEGIIKSENQTLLTLKDMTFSELVDKIKPDKVIGLSTEGKPST
                     YSDVAKLCTDDSCLVVGGFPKGHFADSTKKRIDILLSVDTRPLEIHAVLARTLYEYEK
                     TIFM"
     gene            15860..15932
                     /locus_tag="VGA92_08745"
     tRNA            15860..15932
                     /locus_tag="VGA92_08745"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15894..15896,aa:Gly,seq:tcc)
     gene            complement(15967..17004)
                     /locus_tag="VGA92_08750"
     CDS             complement(15967..17004)
                     /locus_tag="VGA92_08750"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX9846529.1"
                     /translation="MQTKIIKINPKSPQISKIKEAARLIRQGKLVAFPTETVYGLGAN
                     ALNHLAVKKIFEAKGRPSDNPLIIHIYDKNDIQLLAKDIPEITKKLIRQFWPGPLTIV
                     LKKSQIVPKITSGGLDTVAIRMPKNKIANLLIREAGIPIAAPSANFAGKPSPTLARHV
                     WDDLKGRIDLIIDGGQTNIGIESTVIDLSRKRPVMLRPGGVTLEELRKAIGKVDVHPF
                     LRGGKLNSVSRSPGMKYRHYSPLAKLILIEGTKQSVNKKISQLIKTYKKQRKRVGVMT
                     ADKDFSQKADMIKYVGSNFDKIAANLFKTLREFDSKKIDIILVQGISQRGLGFGIMNR
                     LSKAAYKKIKI"
     gene            17093..17395
                     /locus_tag="VGA92_08755"
     CDS             17093..17395
                     /locus_tag="VGA92_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit"
                     /protein_id="HEX9846530.1"
                     /translation="MKPDPKQIAMERIQILINQALANAKKNPTLAQRQASLAKKISTK
                     YRIKLPYQIRMHFCKKCKMFITPGINSRIRLGRSALKSVRITCGFCNHTYRKVISK"
     gene            17428..17880
                     /locus_tag="VGA92_08760"
     CDS             17428..17880
                     /locus_tag="VGA92_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HEX9846531.1"
                     /translation="MAKAYDVPADALISRLAEALKSENIGRPEWTMYVKTGSHKQRPP
                     QDKDWWYKRCASIFRKIYLHGPIGTNDLRIEYGGAKAVGYALPHHRDSGGAIIRNAIH
                     ELEKLGYVEKVEKKGRVVTHQGMKKLDRLASEILDEMAVQNPTLKIYS"
     gene            17890..18165
                     /locus_tag="VGA92_08765"
     CDS             17890..18165
                     /locus_tag="VGA92_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX9846532.1"
                     /translation="MSYEQDDSNPQKEAEITAQKEFILKQLLSPEARLRLNNVRMVKP
                     DLAALVENYLIGMASQGKLQSQITDDQLKQILMSVQQPKRDFKINRR"
     gene            18208..19284
                     /locus_tag="VGA92_08770"
     CDS             18208..19284
                     /locus_tag="VGA92_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9846533.1"
                     /translation="MKGVVYEEYAPDDNYQKILKVKEVPDPKPKATEVVFKVKAAALN
                     YNDIWGMRGQPVAVPLPHISGSDAAGEVVAVGEDVTTLKVGDRVVSHANMSCRVCKAC
                     TEGREYDCTKRTIWGFQTGPNWGAFSELTHLPEVNVLKIPEGVGFDEAAAASMTLLTS
                     WHMLVGRAKIKPGQTVLIMGGGSGVGSFGIQIAKLYNCTVIATASGDKLEKCTKLGAD
                     YAVDHRKEDWNKEVFKITKEIAKTSGGVPGIDVSFDHIGQTHWNQQLTLLKYGATLVS
                     CGATTGYDAKTDLRHVFFKGTNILGSTQGTRAELEDGFYWMGKGKIKAVIDSVYSFEQ
                     AAEAHTKMLTGKGLFGKILLKPEA"
     gene            19290..20153
                     /locus_tag="VGA92_08775"
     CDS             19290..20153
                     /locus_tag="VGA92_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9846534.1"
                     /translation="MTRLFRSLLFVPGNNPRFLEKAKTIPADIVCFDLEDSVPDSEKK
                     NARNLIKEALKSRSDYTPDVYVRTNSPASGKIPDDLDQIVQKGLDGIVIPKVNSAKEL
                     NKIKKTLSSIEKKRKLKPIPLMPSIESAQGVVNAYEIAIESKRVTALVFGVFDLLNDM
                     GIEYTKQPDGAKYARAKIPVDAKAAGVYAIDAIWQDLQDKDGLKQDCIIGKSLGYVGK
                     SIIHPDQIPVTHNAFAPNKAEIEWAQKICDTYLESTKKGRGATVVDGKMIDEVHYKRA
                     KALLEVVKNNH"
     gene            20236..21036
                     /locus_tag="VGA92_08780"
     CDS             20236..21036
                     /locus_tag="VGA92_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9846535.1"
                     /translation="MVDNYEEAANNFLELSSHQRLQIIFRLLEKKSKVTSMAKDLNAT
                     TQEVHRNFARLEDGGFITKNIDGYYSLTTYGNTICSQIPSIIFLSHNRKYFEDHNFGD
                     IPPKFIMRIGQLANSEHITGVTKILEQWKSIYKNADEYIYEMLTEIPLDIIEPKVRRV
                     EKGVKLNYILSEGAVIPKGRKNLLLKLNFAKLIDKGLVERKMKKTVLTLLVLNEKEAT
                     ISFPNIEGEPDITEIFYSTDLMFHEWCLDYFRYCWYASEIFQESKLKE"
     gene            complement(21064..21204)
                     /locus_tag="VGA92_08785"
     CDS             complement(21064..21204)
                     /locus_tag="VGA92_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846536.1"
                     /translation="MKQITKPDHWGYQVRAPFKKVIAWDMLFFVLGIGVGAGIGAFLV
                     TA"
     gene            complement(21369..22238)
                     /locus_tag="VGA92_08790"
     CDS             complement(21369..22238)
                     /locus_tag="VGA92_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX9846537.1"
                     /translation="MVSTQIMEKILFGVAKQWIAGNTINEALAAAKISYKNGMSVIIN
                     RLGEYHTSKQLIEKTIDEYKIVMSSFRRWKVSGGISIKPTQIGLSRSKKECLRNFEAL
                     IKAASSSQTFVWIDMESSDHTDETIEIYQNLFARYERLGVALQANLHRTENDLADLLS
                     MGGKIRLVKGAYRETAKVAFKSKEKVDQNYLKLMKVLFEKGNEFGIATHDSVMIGQAV
                     AISKRYEKKFEFQMLRGIRDELKLNLIKMGFSLAEYIPYGTNWLPYSVRRLKERKRNI
                     LLLGSSFISRHKI"
     gene            22377..22967
                     /locus_tag="VGA92_08795"
     CDS             22377..22967
                     /locus_tag="VGA92_08795"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX9846538.1"
                     /translation="MFTGIIEGIGKILKIEKSTKNRSAVKMTVDLGGHARGLKIGQSV
                     ALNGVCLTVTGISNSKCNFEMIEETTKKTDLGNLKIGGLVNIERSLKVGDRMEGHFVL
                     GHVDGVGTIKKIEKKPKEIKVWFETPKHLARFVVQKGSIAVDGISLTVVDVKKNLASV
                     CLIPHTVQITNFQTRKVGDKINIETDILGKYLLKQE"
     gene            23040..23714
                     /gene="ribB"
                     /locus_tag="VGA92_08800"
     CDS             23040..23714
                     /gene="ribB"
                     /locus_tag="VGA92_08800"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX9846539.1"
                     /translation="MSLQEAIFSLKRGEFVLLYDSGGRENEVDMVVAAQFVTPEHISR
                     MRQNAGGLICIAIKDSFAKSLNLQYMHNILSRSSDLDAESKQMIMGTAPYGDHPTFSI
                     SINHKQTYTGITDSDRALTIKEMATTYHSANPKQSFVSSFTTPGHVPLLLASTGLLAS
                     RQGHTEMSVYLAELAKLTPVTAICEMMDSQTYSALTVEKAQKFAKENAIPFIDGKELL
                     EFSRVH"
     gene            23717..24130
                     /gene="ribH"
                     /locus_tag="VGA92_08805"
     CDS             23717..24130
                     /gene="ribH"
                     /locus_tag="VGA92_08805"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9846540.1"
                     /translation="MNIAIVTAEFNDEITSRMLDVALEKAKSLKLQVKYSCKVPGSYD
                     MPLIVDALLQKKEVDGVVTLGSIIKGQTKHDEVIANATVKSLTELSIKHQKPVSLGIS
                     GPGMQERHAYARIRPVAERAVEAVVKLAKELERIS"
     gene            24127..24882
                     /locus_tag="VGA92_08810"
     CDS             24127..24882
                     /locus_tag="VGA92_08810"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HEX9846541.1"
                     /translation="MIQLTIVKITEYGPWTLTLGSDREHALQMLQADLYREIQKLFSE
                     KNCLVFFNRFDEFFVVTNGLGLDDHVEIQKKLGKISKLNLSMSIGYADNPFDANLRAY
                     EGKKTPVILDEKHSIYGFVNGAADQKVTIMHFDIEDLTSQRKVKSPYEISSTIFKLYA
                     KMSEFFLDKKSLTFFMGGDNFMVVSSDNSKQTAKEFLDLAKKEFDITLNCGIGTARTV
                     REAVNLATKSLDTIREIRTSGKEKPEIYEMSCF"
     gene            24873..26057
                     /locus_tag="VGA92_08815"
     CDS             24873..26057
                     /locus_tag="VGA92_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9846542.1"
                     /translation="MLLKNISILYGKNLEYIPSTNLQISNQTFGKIREKLSREKDTID
                     CEGLLLVPGFVNAHTHIGDSIAKDATLKGTVDSKIHPVLGAKPRILKQTTPRHLMAFM
                     KNTCLSMIKKGITTFVDFREGGLDGVLLLKKVTSQVPIRAVILGRLEYYQDKNQIRKN
                     IPLDKLHKNKLEQILKACDGIGISGPNENSSSVLKYYSKTKKIRAIHSSETTESSQLS
                     EKLTGKTETTRALLLKPHFLVHMTYANDTDLRSVANKTNGIVICPRANATLAEGIPDF
                     SRMTKNGCTVAIGTDNVMINSPDMFREMDYLWKVHMGLHKTRIDPKQILKMATINGGI
                     LLGKKIGSIETGYLADTIFVDKHAIDLEPMHNPHASLVHRASESAIKAVMIGGKIIHG
                     KL"
     gene            26044..26697
                     /locus_tag="VGA92_08820"
     CDS             26044..26697
                     /locus_tag="VGA92_08820"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HEX9846543.1"
                     /translation="MASSRPRIVLSAAMSIDGKIATKTGDSELSSRQDKTRVHRLRAK
                     ADAIIVGIRTVNVDDPLLTVRYTRGKNPTRIVLDSFGTILPSSRIIKTANKVPTIIAV
                     SKKAPKKNLAKLVRYPVKILIAGQNKVELKKLLNILYEQKIKTILLEGGGTLNWEFIR
                     HGLVDELIVTIAPYIVGGKDAITLVEGNGFSKILYSPKLKLHKIARQNNEIVLHYYS"
     gene            complement(26688..27203)
                     /locus_tag="VGA92_08825"
     CDS             complement(26688..27203)
                     /locus_tag="VGA92_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HEX9846544.1"
                     /translation="MVESYEDPFVRIAAMIGGDEYLKVARALLKAEDATDEEIASSTG
                     LRINMVRKVLYDLFGKALITGIRVKDERKGWFVYRWRSRKDEVENFIENQKKKIAERL
                     QQRLDYENSSQFYHCGREDCPRITFETALELFFKCPRCGSVVNLKKNDKLKKALESKI
                     TEIRNDMRRQL"
     gene            complement(27234..27761)
                     /locus_tag="VGA92_08830"
     CDS             complement(27234..27761)
                     /locus_tag="VGA92_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HEX9846545.1"
                     /translation="MKKIEVLRIGQRLVRDDRVTTHVALVSRAFGASKIYMNEVNPEI
                     KDTLNKVNKSWGSDFQIEFIEDWRKLLRSKKKVAKIVHLTMYGENINDVEQKITQEDN
                     VLIVVGAEKVPRDVYEQADYNVAVGNQPHSEISALAVLLDRIQGGKQFDAKYNNAKLE
                     IVPTKKGKKVVAKND"
     gene            complement(27748..28875)
                     /gene="hflX"
                     /locus_tag="VGA92_08835"
     CDS             complement(27748..28875)
                     /gene="hflX"
                     /locus_tag="VGA92_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9846546.1"
                     /translation="MTQTAILITYDNEDAIKEALGLCDSAGYKVAKIIRQKVLDKHKY
                     GIGEGKVLEIKELVTKIRPDVIVFDEVLKPSQSYNLASTLKTNILDRESLILEIFEKR
                     SSSFESHLQIKLAQLRYEMSRAKEKVRLAKMGEQPGFMGIGMYEVDTYYNDIQNRMRS
                     VKSKLAKTGKQRALHREARRRVGLRTVSLAGYTSAGKTTLFNLLTGETKEESPELFTT
                     LSTTTRKIFIEKNPVLVSDTVGFISKLPAYMIEAFKSTLEELRYADVVIVVVDISDSA
                     QELKKKFRSCLKTLADLDVDSEKIIYALNKSDLASPEEIVQKSELLGFENSKKWVPIS
                     SVTKQNVPKLLDLVKKMLELPAQQIKEIPKKSDLAEFYEED"
     gene            complement(28881..29930)
                     /locus_tag="VGA92_08840"
     CDS             complement(28881..29930)
                     /locus_tag="VGA92_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEX9846547.1"
                     /translation="MQEREETRRLQFTGKSSYIVSLPKQWVLELGLKQGDQITITREG
                     KSALKILPAKDQAKSTQVDEAVLEITRDDDNAAIVRKLVSLYFLGYKTIQIKPKTDRL
                     QPGQRNSIKSAVKGMLMGSEIISDSVEGITIQVLVNLLELTVDGAFKRMLHLAKSMLK
                     DALLAVEEANLDLAREVIDSDDEVDRFGFYIIRQLKIAISNEYMLKDMGFTNARQCLG
                     YRLIVKNIERTGDHAVLIAKDLLEFKKSVRKEVMEKIEDLNNFATSVLDQACLALFKE
                     DFVQAELAIKKAAETSKYEKRILESIKSIKDEEEAYRIRRMSENIIRIAEYASDIAEI
                     VLNMNIEKMLKKTKN"
     gene            30078..32246
                     /locus_tag="VGA92_08845"
     CDS             30078..32246
                     /locus_tag="VGA92_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9846548.1"
                     /translation="MAKDPVCGMIVDEKRALNSELGSKTFYFCSPVCQKTFLEPEKEL
                     AKMKKRMYLAASGALALAIIRGGAYLALAAGAIAFTWVPFPEIPFLSYGLLLFFIVTP
                     VQFIGGWSFYVGGYHAIKRRTANMDLLISIGTLTAYIYSTVVLFFPDVIPTEEKYVYF
                     EVSAVIIAFVLLGKYMEEAIKKKSSASVRKLLDLRPAMATVVRDGNEIQIPANQVELG
                     DIMIVKPGEKIPTDGLIVDGESSVDEKMITGESLPVGKTKGDEVIGATLNKHGLLKVK
                     ATKVGKETALSQIIHIVEQAQSSTAKVQRLADSISAKFVPAVVITAVTTFVVWTFLLS
                     DLVSGILTFVAVLIVACPCALGVATPAALMVGVGKGAESGILIRGAEYLERSQKVNTV
                     VLDKTGTLTKGEPEVTDVVNLGTLSEEKLVEFAGAVESGSEHPIAQAVVKKAKESGIL
                     VPALEKFEVLNGLGVKAIVRGNFVYAGNRKMMKKFGIPTEPVEEKMSMLESQGKTAII
                     VAVDDKIEGIIAVADSLKESSPMAVAALKDLGIECIMITGDNEKTAKTIAEKVGIDKV
                     IANVLPSDKASEIKKLQSQGKVVAMVGDGINDAPALAQADIGIAIGSGSDIAKETGGI
                     ILIRDDVMDVSRAIRLSRVTMKKIKQNLFWAFAYNSGAIPIAAIGLLSPILAAAAMAL
                     SSISVIANSSLLKRYKITSGEEKLSFKKEYSQEKPIELIH"
     gene            32332..32796
                     /locus_tag="VGA92_08850"
     CDS             32332..32796
                     /locus_tag="VGA92_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEX9846549.1"
                     /translation="MDPILKLKYTIDALFGAGVSKHLPKDITITFSKKTGRIREVKHN
                     GRLLCTLRIDGGLAITTYFAQILLKSKKFRQNCIEVDEDSKPFVEDGKSVFCKHVTWA
                     GKNILIGADVPVLYQNKVIAVGRAIVNTEMMMYLKRGVAVKVRDSLKSSLEA"
     gene            32803..33144
                     /locus_tag="VGA92_08855"
     CDS             32803..33144
                     /locus_tag="VGA92_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HEX9846550.1"
                     /translation="MMRGGNRQMRRMLDKMGLDMKELPNVQEVIIKTDKKEIIISKPS
                     VTEMKSQENSIFQVIANGYEERELETPVFSEEDIMLVSQQANVTPEVAANALKETNGD
                     LARAILLLSTK"
     gene            33180..33530
                     /locus_tag="VGA92_08860"
     CDS             33180..33530
                     /locus_tag="VGA92_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846551.1"
                     /translation="MKEAAQTAVADSKVSKIIKSLADLENDIDSQNIKIAEMKKSLNS
                     KALKEIDSLKEKVTQTAIKEAESMISETKAKAEQQAKKIASDGAAKLDKLKSTIDSKF
                     DEAVDSVVSTILKP"
     gene            33571..34233
                     /locus_tag="VGA92_08865"
     CDS             33571..34233
                     /locus_tag="VGA92_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846552.1"
                     /translation="MKGISTLEDCRIGIATTYGKPYFQFVKALKFLNLKYDSLLPTEI
                     QSYPGSLILTTQEESPQSASQTILNEEILKEDPTVIQGIIIEKLDSGLNQHELIIGID
                     PGQRIGLSVLYLGKEIERGLYTSPDELAFHVARILGGLRSEKKIVKIGNGNMDVAKKI
                     TTLLNLRFCSHFELEFVDERKTTRKIKNYNKRGERDMMSAKYITQRQGYRRFVLPLSR
                     TG"
     gene            34481..34723
                     /locus_tag="VGA92_08870"
     CDS             34481..34723
                     /locus_tag="VGA92_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846553.1"
                     /translation="MTCKGICSRYKAQKPVGTGRYASGQRRCQICEIFIKWEGLWCPC
                     CGYRLRTKPRNLKYKAKLRARVEAEAQEAEAIAIKA"
     gene            34741..35076
                     /locus_tag="VGA92_08875"
     CDS             34741..35076
                     /locus_tag="VGA92_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846554.1"
                     /translation="MSSSDHRVISVETENRKFDVTIVKYDNGFFISITEGARKFGATI
                     ISIGTGPAPSSSIIIPAKADSLFLKLISQKVASEIRGLCIASLSTQKELGANAGKVLI
                     AKIMEAVRE"
     gene            35073..36404
                     /locus_tag="VGA92_08880"
     CDS             35073..36404
                     /locus_tag="VGA92_08880"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX9846555.1"
                     /translation="MTDIRNFIDHIKKSGQLSIIKKKVSTKYEIAAITAKVDGGNAIL
                     FENIKESKLRLVANLVGTRARFAKAIGASESTIHHKVISAIGHAKKPKIVSGGKFLEN
                     SSRDLSVLPVVTHFEKEPGPFITSSIIYAKNPETGAQNSSFHRLMPLDKTHFSVRMVE
                     GRHLHRTFVDAKEHGKDLKVAITVGVHPTISIAGAYQAEWGKDELDIANTLLAGKLSL
                     TKLPYSGLAVPSGAEIVMEGNILADKTHKEWMVEMLRTYDFARQQPVFELKKLYYRNN
                     AIFHDILSGYAEHRLLMGMPIEAKLNRELKKSVPQTNQVVMSDGGCNWLHAVVQISKK
                     KDSDAKKAIEKTFASHRSLKLVTVVDDDIDPSDPVAVEYAMATRFQADKDLTIIQKVR
                     GSSLDPSSDQKNLLTAKLGIDATRPLSKRKEGFEIAKIPGQEKLSLKKYLK"
     gene            36442..36672
                     /locus_tag="VGA92_08885"
     CDS             36442..36672
                     /locus_tag="VGA92_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846556.1"
                     /translation="MEVKRRSSWWFLLPILFSVIGGIIAYFVIREDDPKKAKDCLLLG
                     AILTAIGIAIFVIPILVGMSFMPHTNMFESSF"
     gene            complement(36684..37064)
                     /locus_tag="VGA92_08890"
     CDS             complement(36684..37064)
                     /locus_tag="VGA92_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846557.1"
                     /translation="MLKGILVHKQLNPLDICKQVLSLDDSIRFVGRIKDRKVIAFARH
                     PEKKPLLDSELGNLAHYHASVKASMEEMFDDALGKTNWMITAKEKVKLITLFLDDGLL
                     IFSIDVKADHDEIIKKIQSLNMPL"
     gene            37266..38516
                     /locus_tag="VGA92_08895"
     CDS             37266..38516
                     /locus_tag="VGA92_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846558.1"
                     /translation="MNDKLKLASLLLILPLFTVGLTTNFQDAEALKSKGTPLSATSSS
                     KVCGDKLCSEIPGGKSAMEEKLSKKTEKMTEKETMMEKNSMVETETMMEEPGYELRLA
                     RTNVPVTIPLHQGWYNGESVYFIITDSSEQKHADVITQNQGWKVELAPFLANAPKKAL
                     STTYVFTNGIEGGGIHGFQSEVFTNTPAQPETYSALTSHVHVTWNDASQADLLDSEAA
                     ILAAEEEGLVTLSDLPVVINMPQVVWPGGQLQVKDDKTLTDDTPYGGGQVLDIDTDEM
                     TVTFVAHRGWGPDGRTIYYIVTDATPSGPAAMMGVASSPTSAALIANSAAVDLFQFMN
                     GAKGTGPLGFQPGIAAAAPGDKNYSPMWRIFAIGWKDPTSASVLETVGDINAYSKAGL
                     IDVNLARPMDSDHIVNCPFIDPFQ"
     gene            complement(38679..39974)
                     /locus_tag="VGA92_08900"
     CDS             complement(38679..39974)
                     /locus_tag="VGA92_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9846559.1"
                     /translation="MSELRRHLGLFQTTMYGVGLILGAGIYALIGSATGEAGNSVWVS
                     FILAAMAAVFTGLSYAELSSMYPKAAAEYSYTKNAFKNNFVAFLVGWLTLFVAIISAS
                     TIALGFGGYFAGLFGAPILISAILVIVVLSFVNFIGIRESSSMNVIFTIIEAAGLGLI
                     IWIGFALFGNSQVDYFEAPHGLMGIFSGFTLVFFAYIGFENIANIAEEIRNPKKVLPR
                     AIILSISITAIIYILVSISAIRVLDWQELGSSIAPLADVVKKALGPQAQIILLLIALF
                     ATTNTVLIMLLSGSRILYGIARDKSLPAIFGIIHQGRKTPWAGVIMIGSLAVAFTFAG
                     DISTIANISVFAIIMVFIAVNFSLIWLRLKEPQAERPFKVPLNVKNFPILPVLGIITP
                     IVGVIQLDVYVILMGIGVVLSGAAFYFIYNRIKKTTKVD"
     gene            40074..40517
                     /locus_tag="VGA92_08905"
     CDS             40074..40517
                     /locus_tag="VGA92_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846560.1"
                     /translation="MNSDKKFIIIGLIGGFIALAAIFGYASQGYVRPFNIFWPEEVRQ
                     TLEFRTLDGRTVVVGTVGNAGTNPELVMRTGDYSYIITVVNKDVISHQLFVEGPNIST
                     KLLEPDERDKIILQSKNEAIFNYYDTANGKEFLGTIRAVKVTPFE"
     gene            40554..41477
                     /locus_tag="VGA92_08910"
     CDS             40554..41477
                     /locus_tag="VGA92_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEX9846561.1"
                     /translation="MISIIGSGKVGSALAFLCGSFALDDVILVNRNEKKAIGEALDAS
                     TTIPATSTISINGTSDYSKIKNSDVVAITASSGVHLQSRTEIMYDQAIMIREISQNIA
                     RYAPEAKILMITNPVDVLTYVMQKERDFPSKNVIGVASSLDSSRFRYLLSKEFGTNQS
                     KITDALVLGEHDDSMVPIFSYAKFNGKPVQDILDGQKKSEITNEVRNYWKYLRDYKGH
                     SVFGIAKSTFDIIKCIIKNETLDIPASTLLDGQFGLSDVCIGVPLTINKNGIAKIHQI
                     KITKNENESLQKSAHVVRNNITKVLEFLKTN"
     gene            complement(41470..42288)
                     /locus_tag="VGA92_08915"
     CDS             complement(41470..42288)
                     /locus_tag="VGA92_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HEX9846562.1"
                     /translation="MKKIMIIKANGEKAPFDPNKVESTCIRAGASPDLAKRIANQVYS
                     KTRQGMSTREIYRMVLHFLSEHGMIAIKHRYRLKEAIMRMGPAGFSFEIYVGQILGHF
                     GYTTKSIRTEMTGRCVKHEVDLVLESIQDGKKWMAECKYHNMPGRYTGLKESLYTHAR
                     FLDLSDKFDGEMLVCNTKVSSDVITYAKCVGQQVLSWKYPTEKGLERMIEEKKLYPIT
                     ILGPTKKELVLFSKNSLMIAKDLVDMPVNEVVNKTGISIKRIIILRNLARQVIS"
     gene            42498..43277
                     /locus_tag="VGA92_08920"
     CDS             42498..43277
                     /locus_tag="VGA92_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9846563.1"
                     /translation="MTNQIIWIGVVVGVFFAGLGIGYAMFYSTQPNFTHMTPQQMQQM
                     MNNPQSIQQMHDLMMNDPNHMQQMHQMMMQSPQHMNTMMGTMMQNSQFGSVMMGQMMN
                     NPNTMNGMVNQMMNNPQFMNTMMGTMMQNQNFQQQYMGPWMMMRDSQFMHNMRGQWFQ
                     ETNLESSAVQTNQVSILADTWRYQSTKAYSPSVIKVSTGTTVTWTNDDTIIHTVTDLG
                     NEFDSGFIQAGETWQYTFDTKDTYHYFCSIHPWMRGTVLVS"
     gene            43349..44038
                     /locus_tag="VGA92_08925"
     CDS             43349..44038
                     /locus_tag="VGA92_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX9846564.1"
                     /translation="MKWHFEDFIYGSFDGSVTTFAVVAGAIGASLSPMIVVILGFANL
                     FADGFAMAVGSYQATKARIEFIQKERKREEWEIDNMEESEKQEIRDIYAKKGFTKELL
                     DEIVRVITARKKVWVDTMMKEELGLIEDDKKPLASATSTIMGFTLIGIIPLIPFVIIY
                     ALGLTSSVSESFTSSVIFTGIAFFIIGIIKGKIVKKPLFQSGLITLLIGGIAATVAYL
                     VGNLISNLVRG"
     gene            complement(44035..>44447)
                     /locus_tag="VGA92_08930"
     CDS             complement(44035..>44447)
                     /locus_tag="VGA92_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9846565.1"
                     /translation="SSATVNEAAKMMEKARVGSVLVLEQNIPVGIMTDRDFAIKIAAH
                     AYPIHTKVKQVMSSPLIHVSPDDEVWIAADLMYSRKIRRLPVLDEDKLLGIVTTTDLI
                     KLYTVCNREDTRKMYYHALTKIFDDHNEEAIASY"
BASE COUNT        14426 a         8901 c         8954 g        12166 t
ORIGIN      
        1 aaggaggcgg agtacaatcc ggagtaacaa atgcctttag agaccttcag gccacagtca
       61 aatcaggcag tgtcacaaaa tcactagaca ttctttcaga tgttagggcg ggtcattact
      121 attcaaaaat aatcccaacc aaaaccggct ccctcgcagt agagctaaag ggaaccataa
      181 acggagttcc agtaaatgaa gaggttgcca tagaggatgt ggaaagcata gacattcttg
      241 catttcctcc aactggctca ggaggatcaa accaagacgt cttggcacta acgaacgcca
      301 tgtcctcact tcaaaaagac gttacggaaa ttaaatccaa aatgggaaac attgcagatg
      361 gaaccaatat tgatctatca aaatcttatg actttgcagt atttggcatg gcacttggcg
      421 cagcaggtgt aatacttgca gtaactgcta tgataaaaag aaaataggaa agaattctaa
      481 agttttagaa tcgctttaga tcttgctgtg acagcaacaa tgcttactat ggcagctgca
      541 agaactatca tggcaattgg accaaactct ggtaccacat ttagtgagat tgtctcacct
      601 attgggccac tccagtttgc ctcatcgtca ggaagtccaa tacctaacat cgtaatttga
      661 acatctacag cactatcgga gctcagagca cttgttgtaa agtcagctga gccagagtgt
      721 acgtgttgac ccatttcagc caaaacctct gttccatctt gtgttgcaga aatatcaaag
      781 ttgacatgtt ctattgcggt tccgccagca tcagtgaatc ctactgtcaa ggccatttct
      841 tcaccttcca ttggtgcgcc tgcctcaacc gtgatcatta ctgagccatc cattgtcatt
      901 tctgttacat gagcagcatc tccatccatc atttcaccat catcttctgc gtgagctgct
      961 tgaacgatga ctgttccttt catccaagca tgaaccatgc agtagtaggg atattccccc
     1021 tccgtttcaa acttgaatgt tacttcttgt cccactccag aaataaggtc tagatcaaaa
     1081 gggaatactc cctgaggacc tcctgtttcc gctgaaccgc tagttacggt atgaggactc
     1141 ccaacagcat catcatcatt tatccacgta acttcggttc caacatcaac agtaatctca
     1201 gctggaacat aacattggtc ttcaccgcaa gcagaacctg taccctgatc cactatgtgg
     1261 attacctgtt cagcatgatc tgcaaaagca gatggtgcaa ctaccatcaa agcgaatatt
     1321 gcaaagatgg agcctatggc tattgtcttc attgaacttg aattaataag ggatccataa
     1381 aaatccttat tctgattcac acaggattta cgctattttc taaatttacg cagttttgct
     1441 attaccagag ctggtataac aaagtacatg ccaacgttta gcagtatgac tccaatccca
     1501 tatcctaaca gttcttgttc agagtcaatg tctacatagt ttagtattga caaagtcgac
     1561 aacattggcg taattgtagc ccttacaaca tgcttgaatg caggattttg tctttcccaa
     1621 tctgccacgg tcgggctaaa tgaatagtaa atgttgttaa atgccgtcat gaacgctgtt
     1681 cctgtactgg tgctaaagac tacatcgtcc cttacttctc tgagcaattg tacttgtggt
     1741 gcaagctcgg acccaaatgc ggcagttgca attaggcatc cgttaccatt tggagtctta
     1801 tcaccatttt cttgagcagc cgcttcaatc attgtagtaa atgtaacggt ttctggagga
     1861 attggttgaa accaaatcag ttctacatca actgttattt tgtgttctcc cttttctttg
     1921 aattcaactg gtatggttat tgaccccgtc ccagtatgct gaagtggtat tgggccaaaa
     1981 actgctactc catcctttgt aacagttacc ttgtagtcta cgtgctcttg aatgccgttt
     2041 gtttttggat ttatgaaatt aatttttagt tttgtcaaat ccccaattac tggttttgca
     2101 ggttcggtgg tgatgtccac aagcaaagtt cccttatcgg ttggaagccg ttgagactct
     2161 gcaaatactg gtgacatcaa aacaggtaac aaaaataata aaaacaaaat tcgcataaac
     2221 ttagtcgcaa atagaatata ttaataattt tctcagaatt ttattactac aataatgcca
     2281 acattattaa tattgattcg ataaattagt aattgatctt gactaagatt tgcataattt
     2341 tgagcatttt ctgcacattt gtgcttttta catcatacac aaactctgct gctgcgcaag
     2401 aaacaaactc gacatcaaaa tcacaaatac aagacattgt ttatactgca aaatttgtgt
     2461 gcggctcaat atctgatgat gatgggccaa ttcgaccagg tcactatgat acctcaatta
     2521 acattctaaa caagaaaggc taccgggttg gaattctctt aactgcagta ataaatgacg
     2581 gtccgtcttc aatagcaatt ttcagaaatc tagagcctga aacatctact ggcatgagct
     2641 gcaagcacat caaagacatg tttggaattg atacaaagga gatagttgaa ggatttgcta
     2701 taattaaggt gcctgtggga tcattacgcg gctttactaa tgagcaaacc attccagatt
     2761 tctcagagga tggaattaac attcttgatg tacaggtatt ctatactgca aatgccctat
     2821 ctaccctgcc acatgaaata gcacgtgaaa aaattgcatt ttatataatc caggacggaa
     2881 caggaaaaat cccaaaggag tcatttagaa aattacttga tgttacaatt ccaacaacat
     2941 ttaatgaaat ttcagatact gagcaaaaag taaaatcaat tcttgctaca aaatacgatc
     3001 ttgacaaaaa tgagctagaa aagctcagaa ttcgcataaa gagcatctca attggagttg
     3061 gtgccctact tgatgatcac gcagtctcat tgcatgtcgt caggccacaa ctcagctcat
     3121 aaactcaact gtagaaatct gcttaataat taaatacagt gaaattttat taattaatgg
     3181 tgagttttaa aaaaattttt gttgcaattt ttgctatttt gctttcagct caagcattta
     3241 ctattgcgca atttgcatat ggggatggcc tcacacaaga aaatcttccg ccagctagtt
     3301 ttggcgacag acaagctgca ctattcatta aaatcagccc cccaattcta actaaagata
     3361 ccgttggaga tacatatcta caacttagat tgtatgacgc aaacacaaac caaccagtcc
     3421 cacacacatc ttacttcatt tcagcatgga aagacgacaa acttcttcta cgaaacctat
     3481 tccatgccca ttctggagaa ctgacgctca aaattgtacc aaaaaacgta gacgtaaacg
     3541 atgttatcgt atatggcgac caagtgccac agatacctgg cgcttggact ggatataacg
     3601 acagggtgga tgtacacgca ccagtcctat tagaatctgg gttataccac tttgagatca
     3661 aaatatttgg tattgattat gaccaaaaca tctttgccga acaagatgta aagaccttca
     3721 actcttggct gagtgttggt gacatctcaa atcatacaat aaaatatggt gcagagtcat
     3781 acccggtatc tatagtgtca tattacgatc gaatagacaa cttcaaattt gacgaaacaa
     3841 aaaaatcaat ttcattttca atgccgttta actgggatac aacacgaatt gagggacaaa
     3901 caatttttgt acatcaagaa gtcagggttc cgcaggcatt caaagaattt gctgatacaa
     3961 ccgcatacga tggtaaagta aacggcaagc caatctttgg aagaatgtta atacttgatc
     4021 catattcaat cgaaggaaca tcaattttac actttttgat aaccaaagca gacatactcg
     4081 atttagctaa aacaattcca ttaagcacaa aaacaatgga ctttacagta tctccagcat
     4141 cagctgttgc caaaaactct tttactgtga atcttgactc tggggctact gctactgtat
     4201 cgtatgatgc aaatcttggg gctagcgaca caatacctct ccagataaca ttctttgacg
     4261 aaaacggcca gattttgaaa tttgtacgcc atggatttag aatagatgac agctcaggaa
     4321 agattctagt tgaaaacgta ggtggtgagc cacaaaagcc aggcatactt tccatagaag
     4381 gaattgatat tcaagaaatc aagtttccag ctcagggaaa ctacaagatg acacttgtcg
     4441 tctttaatca cggcctagat gagctcacaa cataccaagg aataggcagt gcaacatttg
     4501 ccataggagc gggaggccag aacgcaccag actaccaaat tccatcatgg atcaaaaata
     4561 acgcaaaatg gtgggcagaa ggcgcaatca ctgacaatga ctttgtgcag ggaattcaat
     4621 acttgataaa gcagcaaatc ataaagatgc cttcaacaca atcaggcaca ggatcacaag
     4681 taatcccatc atggatcaaa aacgacgcaa aattgtgggc tgaaggaact atcggagatt
     4741 cagactttgt gcaaggaatc caatatttga taaaacaagg aataatcaaa atataatact
     4801 caacaaaaaa tatccaattt tacaaaagtt tttcgtaaaa ccagatttac attattatac
     4861 aataaaacat ttgtactaat catcaatgct tccaataaag gacgaaaatc caaaaccacc
     4921 tggattcaaa cccaaaatga ccattgcact tatcatagta aatgtaattg tcttcttttt
     4981 tgaggtggca gtcaccggac agttctttga gtttagcaat aataatgcgg catcactatt
     5041 ttacaactgg ggggccgttc ccgcatgtat aacaggggag cgctccatat cgatacaaca
     5101 attccaagtc tcctgccctg cgatgccgat gttggggcta ctaagctcaa ctttcctgca
     5161 cggtggacta atgcaccttg gcggcaacat gttgtttttg tggatatttg gagacaacat
     5221 cgagttaaag tttggaaaac tcaaatttct tggaatctat ttgttctggg gaataattgc
     5281 aggccttgca catgtagttg gagacatgaa tagtccaatt ccagcagtgg gtgcatcagg
     5341 tgcaatatca ggcgtgcttg gggcatacct tgtgatgttt ccacgagcgc gagtcatgac
     5401 atttcttatg atgggattct tttggagaat gattcacatt cccgcaaagt ggtttttgcc
     5461 attctggctc atcttccaaa acataattcc gtttttgatt ggtggatttg gttttggtgc
     5521 agggggggta gcctaccttg cgcacattgg tggatttgta atcggactgg caactggata
     5581 tctgtacaaa aagactcacc agtccgaatt tatgtatggt tcaagatacg gatcgagata
     5641 cggatggaag ggagactagc tatcacatac gcataaatca agcaagtcaa acaagcaatc
     5701 atgccattaa tcacagtctc gttgtatcct ggcagaacac aacaacaaaa agaagaattt
     5761 gcaaaagcaa taacaaagtc agcagttgag atactaaaaa caaaagaagc tcacgtaatt
     5821 gttgtctttg aggaaaatcc aaaagaaaac tggtacttgg caggaaactc cctgtaaagt
     5881 gtcagtcttt ttatttacat tgttttttag ataatacatt gaccaaagtc gcagtagtcc
     5941 agttttgtgc atcaacacaa aaagacgtta acttggaaaa aattctcggc tttatcagaa
     6001 aggctgcaga gaggggagca cacctctgtg catttccgga atttatgatg ttttacacaa
     6061 actcaaaaca aacaccaaaa gaacttgcca ctctagctga aacaatcaac ggcaactttg
     6121 taactacaat agccaaagct gcaaagaaaa actcgatcca agttatagga acactgtatg
     6181 aaaaaagcaa aaaaaaggat agggtttatg acacgtcgtt tattgtcgac aaatccggca
     6241 aggttgtatc tacgtacaga aaaattcatc tctatgacgc acttggcttc aaggaatctg
     6301 caaaacttga ggctggctct aaaatctcag gcccagtcaa gacaagtctt ggaaaaacag
     6361 gcatgttaat ctgctacgac ttgaggtttc cagaagtatc acgcatactt gcctctgcag
     6421 gatctgaaat actagttgcg ccatctgcgt gggttaaggg caacaaaaaa gaagaacact
     6481 ggatcacaat taacaagact cgtgcaattg aaaacggatg ttacgtggtt gcaccagatc
     6541 aagtcggaaa catttactgt ggaagaagtc tcgtagttga tccatacgga gaaattttac
     6601 ttgatatgaa aaaaaagcag ggaataggaa ttgtagacat atcgcttgac aaagtaaaac
     6661 aaacaagaaa ggtcttgcca cttttacaaa acagaagaac agatgtttac tcgcttaggc
     6721 cttaggtttt ctgcttgggc agttgatgtt ggagcactgc cgtacccatc tctgtcttct
     6781 tgaatatcga aatagtatct ggggccaggc acacagtgca catggtatct tggtttgaca
     6841 caacattgct ttttgtaata atggtgatga tgctctgcaa ccgttataat agtttgaaca
     6901 ccccatgaat cgtttttttg ttttccttga tctaattacc atgagctttc caactttaca
     6961 gctgggacac gcaactagtc cgttttttgg ctcatttgaa cctaaaataa catactttct
     7021 tttgctttca gaccacgaaa ggtaaccatt cttatcgaaa tactgacgta tttcggttcg
     7081 aaacaagttg attcgtttct gtgttagctt gacagtactt cgaatcggtt ttttcactaa
     7141 aagcgttcta gttgaatttt tcacttgatg aattattcgt tgaacgattt ttatatggat
     7201 ttggtcaggg agtgttgtgg aaaaggtctg cgttcttggt gctggaagca ccaaatatgg
     7261 aaaattagaa gacagcatta ctgatattac tattcaggca tcagttggag caatcgaaag
     7321 tgcaggaata gagtcaaaag agatccaggc aggatacatc tcgaatgttt ttggcattgc
     7381 agacaagcag gttcacctag gaccggtaat catgagcaac cttggcatct ctgagaggcc
     7441 ctcgctatca atcgagtctg cgtgtggaag cggctccgtc tcgtttcgcg aagcatttgc
     7501 taatgttgct gcaggatttt acgatgctgt tttggttgcg ggagtagaaa aggtaacaca
     7561 caccggaaca gagtggacaa ccacatactt ttcatattgt tctgattttt tctatgaggg
     7621 tggtgcaggg gcatcattcc caggtctttt tgcatcaatg gcaagagctt atctgacaga
     7681 attcaaggca acagaggaag acctcgcact tgtagcagta aagaaccatc aaaatggatt
     7741 tttgaatcca aaggcccacc ttcgaaagaa aattacagtt gatgatgtaa tgaaatcagc
     7801 agtagttgca agcccactaa aactatatga ctgttgtccc ttttctgatg gagcaagcgc
     7861 agttatattg tgtagtgaga aatttgcaaa ggcccattct aaaaactaca ttgaagtgat
     7921 tggatcgggt aggggtgggt cacctgctac actgcaaggc cgagatcaaa tgactaccat
     7981 cccaagcaca aagattgccg cagaagctgc ttacaaaatg gctggagtca cgccaaagga
     8041 tattgatttt gcagaggttc atgactgctt tacgattgca gaaatagttg acacagaaga
     8101 ccttggattc tatgcaaaag gacacggagt ggaagcagta agggagggaa gaactgcact
     8161 aaatggcgaa atttcgataa atccgtctgg tgggctcaag gcaaagggtc atccaattgg
     8221 cgcgacaggt gtcggtcagg tagttgaggt ttttgagcag ttaacaggta aggccggtga
     8281 gcgtacggtt agagatgctc gtattggcct gactcacaat tttggcgcaa ctggagcaag
     8341 ctgcgctgtg cacatattca aaagcgtgta agccttggca atgaagcaag agttcataga
     8401 tacaatcaag tcaggcaaga tacttgtcaa aaaatgcacg aaatgtgaga gcctacattt
     8461 ggccacagtc tatttttgcc aaaaatgtgg caataagggg tttgaaaaca agattttgga
     8521 aggaacaggc acagtcgcta catacaccat aattacagtt tcgccggcgg gatttgaaca
     8581 atacactcca tatgcatggg ttgtaatgaa aattgacgga tacgatctta ggatttccgg
     8641 ctttttggga ggaatcagct cgccagctca gctcccgata ggatcaaaag tcaaaattgt
     8701 cggctacgac gatcgtggaa taattttaga aaaacagtaa accaaattta ccgtgttaat
     8761 taacaaaatt caaaaaagat ttttaatagc tccaaaaatg tctcactttc attgtcagtt
     8821 gatgtaatct gtggaaagtg cggagcaaag atctccaata tgaaaatgct caagtcgatc
     8881 aaagatgtaa tgaaacacta caacaacaaa tgtccctcct gtggacagac cctctctaca
     8941 tcagagtttt cggtagaaat cgagaagaac tgatcaaaaa tgttgtttag aatcacgtca
     9001 taaatcttat ttactcctga tctataggca taagcatgag tatcgaggta gccacaggat
     9061 cactagatcc aaagaaaagc ggtggcattt tacaatctac atcaaaacgc gtgaggatga
     9121 ttttttctgt tatggcaagt ccaaacagga tcgatatttt gcgaatttta aactctaagg
     9181 gacccctcac ctattctgag ctcaagtcac ttgctggctt caagtccaaa aaagaaagcg
     9241 gcaagtttgc ataccatcta agaaaactgc taagacaatc cctcgttgcg ttaaacaagt
     9301 ctgagaggcg ttacaccatt acaaaccttg gaaaacttgt tctaagtctt gcaagacaga
     9361 tcgaagaacg ttctatcata gaatctggca agatgtatgt tagaacctca cacgaatcaa
     9421 tcgaggagtt caattcccac aaaattatcc agtctttagt tagagaagga agtcttccat
     9481 tggagctggc acaaaaaatc actgaagaag tggagaaccg aatctacaag tatcaaacta
     9541 catacctaac aggctcgctt attcgtgaga tggtaaactc ggtactctta gagcacggcc
     9601 acgaggagta tagaaacaag cttgccagac ttggcatgcc tgtctttgac gttcaagaga
     9661 tgctcacaaa cgtagataac attgacaacg gcgcacaagg actgtttttt aaggcaggcc
     9721 agactgtatt tgcagaaaac ctactactta acatgcttcc aaaagacgtt gcagactcgc
     9781 acctttcagg cgatattcat atctcaaatc caggcatatg gtcgcttttg ccagacacga
     9841 tattctttaa tgtaaaggag gtaatcgagg acggaataga cctaaagggc aaatatctgg
     9901 gtgtgagccg tattcctaca atcaaaacca ttgataattt gatggcgtct ttgtccatga
     9961 taatatccct ggcttcaaag gaggcatcaa aagaaatcat catggatgga ttagtacagc
    10021 tttgctcaaa gcacgcaaaa aatatcacag aacttgagga aaaattagta ggcgcattta
    10081 ccacctcatc tgttttgtcc aagtatacta aggagcctac actggtctca tttagaatcc
    10141 agcttggctc agaacccaaa atagtgcagg caatccttgg cgcatacaaa aactatgtca
    10201 aaatgacccc ggttccacaa ataggactaa taattgatca tgccgtaggc aagatatctg
    10261 acgtgtctgg agtcgttgcc gagatcatct cgcttggcgg caaggtattg ttcacaaaag
    10321 acgagacgtc atacagtggt gttgtgagga tcaagggcaa aaacagcact tcttcaatta
    10381 atctacaatc attgacaatt aacctaccaa gacttgcctt tgaatcaaac aaagatgaga
    10441 catatttcag agcaaggctt gccctgctaa tgaagccagc acttgccgca atgtcacttc
    10501 gcaaaaaaga tatttctgat cttacgagga ggggactcaa cccagtcttg gcagcaaaca
    10561 cgcaatacat gcaacgaagc tcagtctgtc tggtactaaa cctcatagga ctaaaagaag
    10621 cagtattcaa tatacttggt tacaataacg acaaggaagg acaagaaatt atttacaaag
    10681 taatccagac tgcagtagat gttgctgaaa agaagggcaa ggaaatgggt gatgcagtaa
    10741 ttgtggcaat gactgaatct gacggaacga gtcgatttgg taccctcgat ggagaaaaat
    10801 acggcaaaat gtctgttctc agatcccttg atggtgcaac ctactcagaa ggtgtggttc
    10861 ttgcatcctt agaacttgat tccatgaacg caaaggctga aaaaatcgta gaagcaaaca
    10921 agacttcaaa gctcttaaac ggaggcctgc tagtcaaact ccaatttgag aaggagtcaa
    10981 aggtcgagga tataaagaaa gcactcgaaa aggcaggcga tctggtaggt gcgtttagac
    11041 caagcaagga agttccgatc tgtgggaact gtggctttaa ggatgaaaaa ctctttgaaa
    11101 aatgtcctat ctgcaaatcc ccgtatgtta tcagctaaat cgaaatccgc aacatctatt
    11161 ttcgtcaaat aaaaattaat ccttgttgga aaaaagatcg cctctaaaac ttgctgaatt
    11221 cgcaatccat cctaggaaca acccttgcga aatttcttgg aatggtgtaa acattgttga
    11281 gctaaaatct gaaatctttg tcagtcaaga ttatatccga ttcgatatta cctagtgagg
    11341 ggagacatta tgacaacaga tttttctcaa atagaaagtt cgatcgtaga cgtgaaaaag
    11401 cgagatggta gaattaccaa tttcaataaa gaaaagatca ccaatgcgat ctacaaggca
    11461 ttaattgcca atggcaaccc agacaggaaa atagccgagg atctaacaag cggggttttg
    11521 accaagctga ttgaacaggg attctctgtc ggaagccctc caagcgttga agacgtgcaa
    11581 gacatggtgg aatcaacact aatcgaaaag ggacttggcg acattgcaaa atcctacatt
    11641 ttgtacagac acgagcgtcg caaactacga gaagacaaaa tgaaggtcct taacacaaag
    11701 tctttggatt ctgtcgcaaa gacatttgat ctaaattgcc tacgagttct ggcatcaaga
    11761 tatcttttac gtaacaacaa aaacgaaatc actgaaagcc caagtcaaat gtttgagcgc
    11821 gttgcaatac ttgtaggcat cggagatctt ctttacgatg cagaacttta ttctaaagac
    11881 gcaagcgcac accaagacat cgaagaggca agacgatacc taacaaagct tgatgatttc
    11941 gaatacaaat tcaaaatagg aagctattat ctgaacaaat accaattcag gggcctcatc
    12001 aaccactaca tctacctagc aaagcgaggc cagatgaaaa agagctttaa agaagttctc
    12061 acacttatcg ctgcaaagaa attcgacaac tatgcagaac gcattcagga atactatgac
    12121 cttatgacca agcaggactt tttaccaaac tcgcctacaa tgatgaacgc cggtgcacgc
    12181 ctaggacaac tgtctgcctg ctttgtgctt ggcatgcctg atgatatgaa tgacataatg
    12241 aagtcaacat cggatgctgc gctcatcttc aagtcaggcg gaggggttgg catcaattat
    12301 tctgatctta gacacgaagg ggacattgtt gcatccacct ctggggtggc atcaggaccg
    12361 gtctcattta tgaacataat aaacacagta actgaggttg tcaagcaggg aggaaaaaga
    12421 cgcggggcaa acatgggaat cctggagtct tggcacccag acattgaaaa attcatcaca
    12481 aacaaaaccc aagcaggaat acttgaaaac tttaatgtca gcgttggaat ctgggaggac
    12541 ttttggtctg cacttgttga cagccaagat ggcaagtata cacttcgcag cccgcgcgac
    12601 aaaacgccag tacgtgaagt caatgcacac cagctaattg atcttattgc aatgtcagca
    12661 tggaggagcg cagagccagg tctcatcttc tttgataaca tcaacaagta caacgtcttt
    12721 gcaaaggcaa ggggtatgcc cctgcgggcc accaatccat gcggcgaaca atcattgtat
    12781 ccatacgagt cgtgcaatct aggttctatc aatttagcaa atcttgtgaa aagaaaagca
    12841 gacggtcaat acgaatttga ctggcaaaga tacgaggaaa caattcgcaa gacaacacga
    12901 ttcttggaca acgtaattga catgaacaat tatccggttc cagaaatcga ccaagcatca
    12961 aaggaatcaa gaagaatagg gctaggtgtg atgggagtag cggatctttt gtacaagctt
    13021 agaattccat acaactccaa ggaaggatac gagttccaat caaaacttgc agaagcactc
    13081 acatattact ccatggagga aagcgttgca cttgcgcact ctagaggtga atttgatcta
    13141 tgttcaaaaa ctgaatatcc agaaggtaag attccagtcg caggatatta tgaaaagcca
    13201 ataggtgagc attattatga ctgggacgca ctaatctcca agattcaaaa acacggaatc
    13261 cgtaacgtct tggcaaccac agttgcacca actggaaccc tttctatgat tgcagactgc
    13321 tcaaatggaa tggagccaac atttgcacta gtatttgaaa agcgtgtaac cgttggtaga
    13381 ttcttttaca caaacaagat ctttgagaat gttttgcgag aaaacggatt atacagcgac
    13441 gaactcttgg caaaaattgc agacaactat ggctcagtaa gagggctggc tgaaattcca
    13501 gaatggatcc aaaacatctt tgttactgca atggacatac actggtctga ccacctgatg
    13561 gcccaagcag tatggcagga gtggattgga aacgcaattg caaagacaat caacatgcca
    13621 cacgatgtat catcagatga tgtcaaggcg gcatacctgt tggcgcacga gcttggacta
    13681 aagggaatta cagtgtatcg tgacgggtca cgacacaccc aggtactgca tatgatgagc
    13741 gaaaacgcag taaagacctt cgatgtgtta ccaagcgaat atttgcatga gtacattgga
    13801 aaacacatca aaaacacata catcagagcc caagtcaaca acgcacttga actcaaaatt
    13861 cctgaagagc cactaaagga aagcgcactc gaagagataa gagaagacat gctctgccct
    13921 tcctgcaaga ataatcttgt atttgttgaa gggtgtagca tctgtatcga atgcggattc
    13981 tctggctgca catcaggcta aatccaacag aaccatccat cataactctt tttactctca
    14041 atttttactg gcacaattgt gaacaagaaa atttttgcgg caattggtat tgcactagca
    14101 gtagctatcg gcattttggc tgtgctgcca ggctcaccac cacaagattt accggttgca
    14161 aaaaatgaaa agctcggcct tataattaac acgccaggca gtgctgttac gctggatcag
    14221 ctaacgcgtg tttattctga ggcatcagcc gcaggcattg gaagaaacaa tctgtatttg
    14281 ttttggaata acattgaacc gcaaccagac cagtacaact ggagggatac cgacattttg
    14341 atgagcctta acaaaaataa caatctcgag gtaacactgt atttttccat cgttaatggc
    14401 agaatcatcg gtccataccc tgattggatg ggcgcaccag gctttggaac aatacttgag
    14461 caaaatgcag taaaaacgct tgacgcaatc atatcaagat atggaataat tgattatgtg
    14521 ataattggag gtaatcttga ttcttacttt gatgatacgg aaggttctgt aggtctatat
    14581 gaagagtttt tcacaaacgt ctacactgaa ctaaagcaaa aacatccaca agtcaaaatt
    14641 ggtaatgcgt tttcactaaa caacgtactc gacaaaaacc tggaacatta tgtgactgat
    14701 ctgagtcaga ttggcaattt tgtggcattt acgtatatgc cagtagacag actaaatgat
    14761 attaccaaaa caccgcaaga agcacaagaa gacctgcaaa aaatgtttga gcttgtacct
    14821 gacaaaaaaa ttgctgtctt tgagataagc tggagcacat ctgatcttgt aaatggaaat
    14881 gaggcagcac aagccgaatt catcaaaaca gcatacgagt tctacagaca aaatgagtct
    14941 aaaatcgagt ttttcacttg gtaccgtcaa tatgacaggc cagaaggtag ctgcgcaatt
    15001 gagcaaaact ttccagaatc ctcagtcaca attgctggtg accaatatgt aagagaaagg
    15061 cttgcaagct atacatgtga tgcagggcta atcaaaattg atggctccca caagtctggc
    15121 tggtctgagc tcaaaagaca aatacagtca agcagatgat cttctaaaat gctttctcta
    15181 ataatcgcag aatcatcatt agagctagtt ccgcgggagc tacaacgcca caacgcggtc
    15241 acttcatact gcagaaaaat gggcaaacgg ccatcagagg ttttgcttga taattcttgg
    15301 cactttggcg caatgaaggg aattgaaaat gaaattaaac gtggtcggcc agacattgtg
    15361 catttctgcc ttcttgaagc ttgcaccatt ccattatacg aagaagacga aataacagtg
    15421 tatgtccata ctattaacga tcaagtgatc caggtgggaa agaaagtgag gttgccaaaa
    15481 tcctatcata ggttcgctgg cataattgaa aaattatttt tagaagggat aatcaaaagt
    15541 gaaaatcaaa cactgcttac cctcaaagac atgacatttt ccgaattggt tgataaaata
    15601 aaaccagata aggtaattgg actgtccacc gagggaaaac caagcaccta ttctgatgtt
    15661 gcaaaactgt gtacggacga ctcttgtctt gtagttggtg gattcccaaa gggacacttt
    15721 gcagattcta ccaaaaaaag aattgatatt ctgctcagtg tcgatacaag accgcttgaa
    15781 atacatgcag ttttggcccg aactttgtat gagtatgaaa aaaccatttt tatgtaggcg
    15841 caagacgctt gacttttgtg cggccgtagt atagcctggt agtacgctgg ccttccaagc
    15901 ccgttacccg ggttcaaatc ccggcggccg cacctgaaaa actattttta attattgaga
    15961 aatttttcaa attttgatct ttttgtatgc ggccttgcta agcctattca tgatcccaaa
    16021 gcctagacct ctctgactaa tcccttgcac caaaatgata tcgattttct ttgaatcaaa
    16081 ctccctgagc gttttaaaca agttcgcggc aattttgtca aaattacttc caacgtattt
    16141 tatcatgtct gctttttgag aaaaatcttt gtcagcagtc attaccccta ctcgttttcg
    16201 ttgctttttg tacgtcttga taagctgaga aatctttttg tttacacttt gttttgtacc
    16261 ctcaatcaaa atcagcttgg caagaggcga ataatgccga tacttcattc cgggggaacg
    16321 agaaacagaa tttagttttc cacccctcag aaatggatga acatctactt tgccaatagc
    16381 ttttcgcagc tcttctagtg ttactccgcc gggtcgcaac atcacgggtc tctttctgct
    16441 aagatcaata actgtagatt caattccaat gtttgtctga cctccatcaa ttatcaaatc
    16501 tattctgccc ttcaaatcat cccacacatg tcttgcgagt gtggggcttg gctttccagc
    16561 aaagtttgcc gaaggtgcag caatgggaat gccagcttct cttatcaaaa gatttgcaat
    16621 cttatttttt ggcatcctaa ttgctacggt atcgagtccc ccagatgtga tctttggaac
    16681 aatctgtgat tttttcaaaa ctatggtgag cgggccaggc caaaattgtc taatgagctt
    16741 ctttgtgatt tctggaatgt ctttggcaag aagctgaatg tcgtttttat cataaatgtg
    16801 tattatgagg gggttatcag aaggtcttcc ctttgcctca aagatttttt ttactgcaag
    16861 atggtttagc gcatttgctc caagcccata aactgtctct gttggaaatg caacgagctt
    16921 tccctgcctg attaatctgg cagcttcttt tatctttgag atttgagggc ttttaggatt
    16981 aatcttgata atctttgtct gcatcttgat tttagaaaag aattcgtgca ggcatattaa
    17041 cctacaaaat tttgggatcg aattattaaa ggaatttttc tattcttatt ctatgaagcc
    17101 tgacccaaag caaatagcaa tggagagaat ccaaattcta atcaatcagg cacttgccaa
    17161 tgcaaaaaaa aatccaacgc tagctcaaag acaggccagc ctggcaaaaa aaatcagcac
    17221 caaatacaga atcaaactac catatcaaat cagaatgcat ttttgtaaaa aatgcaagat
    17281 gttcattaca cctggaataa attctagaat tagactcggc agatctgctc tcaaatcagt
    17341 acgaataacc tgtggctttt gtaaccacac ctaccgcaaa gttatttcta aatgatttat
    17401 aagtcgagtt aaaagtttga ttttcacatg gcaaaagctt acgatgtacc agcagacgca
    17461 ctcatatcaa gactagctga ggcactaaaa agtgaaaaca ttggaaggcc agaatggaca
    17521 atgtatgtca agacaggctc acacaagcaa agacctccgc aagacaagga ttggtggtac
    17581 aagcgatgcg catctatttt tagaaagatc taccttcacg gcccaattgg aactaatgac
    17641 ttgagaatag aatatggggg ggcaaaggca gtaggatatg cactacctca ccacagagat
    17701 tctggcggcg caatcataag aaatgcaata cacgagcttg aaaagctggg ttatgtggaa
    17761 aaagtcgaaa aaaagggcag agtagtcaca caccaaggaa tgaaaaaact tgacagactt
    17821 gcatctgaga tcttagacga gatggcagta caaaacccaa cactgaagat ctattcatag
    17881 tgaaacacta tgagctatga gcaagacgat tcaaatcctc aaaaagaagc cgaaattacg
    17941 gcacaaaaag aattcatact aaagcaactc ctatcaccag aagcaagact aagactgaac
    18001 aatgtcagaa tggtcaagcc agacttggca gcacttgttg aaaattatct gatcggaatg
    18061 gcctcacaag gaaaactcca gtcccagata acagacgacc agctaaaaca aattctaatg
    18121 tctgtccagc aaccaaaacg agatttcaaa atcaacagaa gataaactca aacaaaatat
    18181 aattaggggg tatctgggat ccaactcatg aaaggcgtag tttatgaaga atatgcacca
    18241 gatgataatt atcaaaaaat cctcaaagta aaagaagttc ctgacccaaa acccaaagca
    18301 accgaagttg tcttcaaagt aaaagcagca gccctaaact ataacgacat ctggggaatg
    18361 cgtggacaac cagtagctgt tccattacct cacatttctg gctctgatgc cgcaggtgaa
    18421 gttgtggcag ttggtgagga tgtaacaacc ctcaaagttg gcgatagagt ggtttctcat
    18481 gcaaacatgt cgtgtcgagt ctgcaaggcg tgtacagaag gacgcgaata tgactgtact
    18541 aagagaacta tctggggatt ccagacaggt ccaaattggg gcgcattttc tgagcttacc
    18601 catcttccag aggtaaacgt tctgaaaatt ccagaaggtg tgggttttga tgaagcggca
    18661 gcagcttcaa tgacactatt aacatcatgg cacatgctag ttggcagagc caaaatcaaa
    18721 ccaggtcaaa ccgtcctaat tatgggtggt ggctcaggtg tcggctcgtt tggaatccag
    18781 attgccaaac tctataactg cacagttatt gcaactgcat caggcgacaa gctcgaaaaa
    18841 tgcacaaagc ttggagcaga ttatgcagta gaccacagaa aagaagactg gaacaaagag
    18901 gtattcaaaa taacaaaaga aattgccaaa acaagtggag gtgtaccagg aatagatgta
    18961 agttttgatc acattggtca gacccactgg aaccaacagc tgacgcttct caaatacggc
    19021 gcaacactgg taagctgtgg cgcaactact ggttatgacg caaagactga tctaagacac
    19081 gtcttcttta aaggaacaaa catccttggt tctacgcaag gaacaagggc agaattagag
    19141 gatggatttt actggatggg caaaggaaag atcaaagcag taattgattc ggtgtattca
    19201 tttgagcaag ctgcagaagc tcacacaaag atgctaactg gtaaaggcct ctttggtaaa
    19261 atcctcctaa agccagaagc ctaggtctta tgacaaggct ttttcgcagc cttttatttg
    19321 tgccaggaaa taacccgcgt tttttagaaa aggcaaagac cattccagca gacattgttt
    19381 gctttgacct cgaagactcg gtgcctgact ctgaaaagaa aaatgccaga aatctaatca
    19441 aagaggcact caaatcacgc tcagactata cgccagatgt ctatgtgagg acaaattctc
    19501 cagcgtctgg aaaaattcct gacgatttgg atcaaatagt gcaaaaagga cttgatggca
    19561 ttgtcatacc aaaggtaaac tctgcaaaag agctaaacaa aataaaaaaa actctatcaa
    19621 gtattgaaaa aaaacgcaag ctcaaaccaa ttcccttaat gccgtcaatt gaatcagctc
    19681 agggggttgt taatgcatat gaaattgcga tagaaagcaa gagggtaact gcattggtgt
    19741 ttggcgtctt tgatctgcta aatgatatgg gaatagagta tacaaaacaa ccagatggcg
    19801 caaagtatgc aagagccaag ataccagtag acgctaaagc agcaggagtt tatgctattg
    19861 atgcaatctg gcaggaccta caagacaagg atggcctaaa acaagactgt atcatcggca
    19921 aaagccttgg ttatgtggga aaaagcatca tacatcctga tcagatacca gtaacacata
    19981 acgcgtttgc accaaacaaa gccgaaatag agtgggcaca aaaaatttgt gacacatact
    20041 tggaatcaac gaaaaaagga agaggtgcaa cagtagtaga tggtaagatg atcgacgagg
    20101 ttcactacaa aagagccaaa gcgcttttgg aagtggtaaa aaacaaccac taagccttgc
    20161 aatatcaacc tgataatgtc ttgatctcac cgttttttta tcggttctta attaccatat
    20221 gtgaggtaaa acagtgtggt agataattac gaagaggcag ccaacaattt cttggaatta
    20281 tcaagccatc aaaggctgca aatcattttc aggctattag agaaaaaatc caaggtgaca
    20341 tcaatggcaa aagatcttaa cgccacaact caagaagtac acagaaattt tgctagatta
    20401 gaggacggtg gttttatcac aaaaaacatt gatggatatt actcgcttac cacatacgga
    20461 aataccattt gttcgcagat accatcaata atttttctct cacacaaccg caagtatttt
    20521 gaggatcaca atttcggtga cattccacca aaattcataa tgagaattgg gcagctggcc
    20581 aactcagaac acatcacagg cgtcacaaaa atacttgaac agtggaaatc aatttacaaa
    20641 aacgcagatg agtatatcta tgaaatgcta actgagattc cgcttgatat aattgagcca
    20701 aaggtaagaa gagtggaaaa aggcgtcaaa ctcaactata ttttgtcaga gggcgcagtg
    20761 ataccaaagg gaagaaaaaa cctattgcta aagctaaatt ttgccaaact gattgataag
    20821 ggccttgttg aaagaaaaat gaaaaagacc gttcttacat tgcttgttct taacgaaaaa
    20881 gaagcaacca tatcttttcc aaacattgaa ggcgagccag acatcacaga gatattttac
    20941 agtacagact tgatgtttca tgaatggtgt cttgattact ttagatactg ctggtatgca
    21001 tcagaaattt ttcaagaaag caagctaaaa gaataatcct acactaaaaa aaggaaaagt
    21061 ttcctaggct gttactagga atgcgccaat tccggcacct acaccaatgc ctaagacaaa
    21121 aaagagcatg tcccaagcaa tgactttttt gaatggtgca cgaacctgat agccccagtg
    21181 atctggcttt gttatctgtt tcatgattat actgcccata tctaattgat agcaccttgt
    21241 cagctaggtt gttgattgat aagctagttg ttgatagcta aaaccaagat gaggacaaaa
    21301 tcggacttac tatgacttac tttcaaattt gacttaccat ttttgtcgtt atcctgatcc
    21361 aagttttctc aaattttatg gcgtgaaata aaggagcttc caagcagcaa gatgtttcgt
    21421 tttctttcct ttagacgacg tactgaataa ggaagccagt ttgtaccata tggtatgtat
    21481 tctgcaagac taaatcccat tttgataaga tttagtttta gctcatctct aattccacga
    21541 agcatttgaa actcgaattt tttttcatat ctttttgaaa tcgcaacggc ttgtccgatc
    21601 atcacagaat catgagtagc aatgccaaac tcgttgcctt tctcaaaaag caccttcatt
    21661 agttttagat aattctgatc aactttttct tttgatttga atgccacctt ggcagtttct
    21721 ctatacgctc cctttacaag acgaatttta ccgcccattg aaagcagatc cgcaagatca
    21781 ttttcagttc ggtgtaagtt agcttgtagt gctactccaa gcctttcata tcttgcaaaa
    21841 agattctgat agatttctat tgtttcgtct gtatggtctg aggattccat gtcaatccac
    21901 acaaaggtct gggatgacga tgcagccttg attagtgctt caaagttacg caggcattcc
    21961 tttttgcttc gtgacaggcc aatctgtgtt ggctttattg agatgcctcc acttactttc
    22021 catcttctaa aagacgacat gactatcttg tattcatcaa tggttttttc gattagttgt
    22081 tttgaggtat ggtattcgcc taatctgttt attattacac tcatgccatt tttgtatgat
    22141 atttttgcag ccgctaacgc ctcattaatg gtatttccag ctatccattg tttagcaaca
    22201 ccaaaaagaa tcttttccat gatttgtgta gataccaata gatcagaatc ggccttcctc
    22261 tgtaaaaata ttcgttagaa cctaatggat gaaattctca tgtgtggaag agccaaaaat
    22321 aagacataga aatctaataa atcaatttat acctaaatca tacacgttaa acagagttgt
    22381 ttactggaat aatagaaggc ataggaaaaa tactcaaaat tgaaaaaagt accaaaaaca
    22441 gaagcgctgt caaaatgaca gttgatcttg gaggacatgc gaggggactc aaaataggac
    22501 aaagtgttgc attaaacgga gtctgtctta cagtcactgg catttcaaac tctaaatgta
    22561 attttgagat gatagaagag acaacaaaga aaaccgatct tggcaatctc aagataggtg
    22621 gattagtcaa catagagcga agtctaaaag taggggatag aatggaagga cattttgttt
    22681 taggccatgt cgacggtgta ggtacaataa aaaaaattga aaaaaagcca aaagagatca
    22741 aagtctggtt tgaaacacca aaacacctcg caagatttgt agtccaaaag ggatctattg
    22801 cagttgatgg aatcagtctg acagtagttg atgtgaaaaa aaaccttgca tcggtctgtc
    22861 ttattcccca cactgtccag ataactaatt ttcagacgcg aaaagtcggc gacaagatta
    22921 atattgaaac tgacatttta gggaaatatc ttctaaaaca ggaataaatt actaccaatt
    22981 tagtggtaat taccaatttt ttagtaaact ttataattgt tttaaagcaa ttttcagtta
    23041 tgagtcttca agaagcaatc ttctcactaa agcgcggtga gtttgtacta ctgtatgatt
    23101 ctggagggcg tgaaaacgaa gtagacatgg tagtagctgc ccaatttgta acacctgagc
    23161 acatctcaag gatgcgacaa aacgcaggcg gtctcatatg cattgcaatc aaagactctt
    23221 ttgcaaaatc gctcaaccta cagtatatgc acaacatctt gtcccgctca tctgatcttg
    23281 acgctgaatc taagcagatg ataatgggta ctgctccata tggagatcac ccaacatttt
    23341 ctatatcgat taatcacaaa cagacctata ctggaattac cgatagcgac agggctctta
    23401 ccatcaaaga aatggcaaca acatatcatt ccgctaatcc aaagcaaagc tttgtatcat
    23461 catttacaac tcccggtcat gtgccattgt tgctagcatc aactggatta cttgcaagca
    23521 gacaaggtca tactgaaatg tcagtgtatc ttgcagagct tgccaaactt accccagtta
    23581 ctgctatttg tgagatgatg gattctcaaa catattcagc tttgacagta gaaaaggcac
    23641 aaaaatttgc caaagaaaac gccattccat ttattgatgg caaagaattg ctagagttct
    23701 caagggtgca ctaattttga atattgcaat tgtgacggcg gagttcaacg acgagataac
    23761 atcgaggatg ctcgatgtgg cactagaaaa agcaaaatca ctaaaacttc aagtcaagta
    23821 ttcctgtaag gttcctggtt cctatgacat gccattaata gttgatgcat tgcttcaaaa
    23881 aaaagaagtt gacggcgtag ttacgctagg ttcgataata aagggtcaaa caaaacacga
    23941 cgaagtaatt gcaaatgcta ctgtcaaatc actcacagaa ctgtcaataa agcatcaaaa
    24001 accagtatct ttgggtatat ctggcccagg catgcaggaa cgtcatgcct acgcacgaat
    24061 aaggccagtc gcagaacggg cagtagaggc agtagtaaag cttgccaagg agttggagag
    24121 aatttcttga ttcagcttac catagtcaaa atcactgaat atggtccgtg gacacttact
    24181 cttggcagcg acagagagca cgcactgcaa atgctacagg cagaccttta cagagaaatc
    24241 caaaagctgt tctctgagaa aaactgcctt gtatttttca acagatttga cgagttcttt
    24301 gtagtgacaa atggtttggg tcttgatgat catgtagaaa ttcaaaaaaa attgggaaaa
    24361 atctccaagc taaatctttc aatgtcaatt ggatacgcag ataatccatt tgatgcaaac
    24421 cttagagcat atgagggaaa aaagacacct gttatcttgg atgaaaaaca cagcatctat
    24481 ggctttgtaa atggagcagc agaccagaaa gtgaccataa tgcattttga tattgaagac
    24541 ctcacatcac aacgtaaagt caagtcgccc tatgagatct cttcaacaat ctttaaactg
    24601 tatgcaaaaa tgtccgaatt ctttttggat aaaaaatcat tgacattttt tatgggggga
    24661 gacaacttta tggtggtatc atcagataat tcaaaacaaa ccgcaaaaga atttctggat
    24721 ttggcaaaaa aagaatttga tatcacgcta aattgtggaa taggtacggc aaggactgta
    24781 agggaggcag ttaatcttgc aacaaaatcc cttgatacca tcagagagat tagaacatct
    24841 ggaaaagaaa agccagagat ctatgaaatg tcatgctttt aaaaaacatc agtattctat
    24901 atggaaaaaa tctagaatac attccttcaa ctaatctgca aatctcaaac caaacctttg
    24961 gaaaaatcag agaaaaacta tctagggaaa aagacaccat tgattgcgag ggcttgcttt
    25021 tagtgccagg ttttgtaaat gcgcatacgc acattggtga ttctatagcc aaagatgcca
    25081 cactcaaagg caccgtagat agtaaaatcc accctgtttt aggcgcaaaa ccacgaatcc
    25141 tgaagcaaac cacacctagg catcttatgg cttttatgaa aaacacatgc ctttctatga
    25201 taaaaaaagg aatcacgaca tttgtagatt ttcgcgaagg cggtcttgat ggagtgttgt
    25261 tactcaaaaa ggtaacatcc caagtaccaa tccgagctgt gattttaggc agactagagt
    25321 actatcaaga caaaaaccaa attcgaaaaa acattccact tgataaatta cataaaaaca
    25381 aactggaaca aattctcaaa gcatgtgatg gaattggaat aagcgggcca aatgaaaaca
    25441 gcagttctgt actgaaatat tattctaaaa caaaaaagat ccgtgcaatc cacagctcag
    25501 aaaccacgga aagctcgcag ttatcagaaa aactaacggg aaaaacagaa actacaagag
    25561 cactgctact aaaaccacat tttcttgtac acatgacata cgccaatgac actgatctga
    25621 gaagcgtagc caacaaaacc aacggtattg ttatctgtcc tagggcaaat gctacactag
    25681 cagaggggat cccagacttt tcccgaatga caaaaaacgg atgtactgta gcgattggta
    25741 cagacaacgt gatgataaac tcacccgaca tgtttcgaga aatggattat ctttggaagg
    25801 tgcacatggg actacacaaa acaagaattg atccaaaaca aatactcaaa atggcaacaa
    25861 taaacggcgg aatcttactt ggaaaaaaaa ttggttcaat tgaaacaggt tatttggcag
    25921 acacgatatt tgtggacaaa cacgcaattg atttggagcc aatgcataat ccacacgcat
    25981 ctctggttca cagggcttcg gagtctgcca taaaggcagt catgattgga ggaaaaatca
    26041 ttcatggcaa gctctaggcc acgtatagtt ttgagtgctg caatgagcat tgatggcaaa
    26101 atagccacga aaacaggaga ttctgagctc tcctcaaggc aagacaaaac acgggttcat
    26161 aggctgcgag ccaaagcaga tgcaattatt gttggaataa ggacagttaa tgtcgacgat
    26221 cctctcctga ctgtcagata cacaaggggg aaaaacccaa caagaatagt tttggattct
    26281 tttggtacaa ttttaccaag ctcaagaata atcaaaacag caaacaaggt tccaacaatt
    26341 atagccgtat caaaaaaggc cccaaagaaa aatctggcaa aacttgttag atatccagtc
    26401 aaaattctaa tagctggaca aaataaagtc gagctcaaaa aacttctcaa catattatac
    26461 gaacaaaaaa taaaaacaat cctgcttgaa ggtggcggca ccctgaactg ggagtttatc
    26521 agacatggat tagtagatga gctgattgtt accatagcac cttacattgt ggggggaaaa
    26581 gacgcaataa cactagtaga aggaaacggc ttttctaaaa ttctctattc accaaagcta
    26641 aaactgcaca aaatagctag acaaaacaac gaaatcgtat tgcactatta cagctgacga
    26701 cgcatatcat ttctgatttc agtaattttt gattctaatg cctttttgag cttgtcgttt
    26761 ttctttaggt tgactacgct accacacctt ggacatttga aaaacaactc aagtgcagtc
    26821 tcaaacgtaa ttcttgggca gtcctctctt ccacaatgat aaaactgcga cgaattctca
    26881 tagtctagtc tttgctggag ccgctcggca atcttttttt tctggttttc gataaagttc
    26941 tcaacttcgt ctttgcggga gcgccatctg tacacaaacc agccctttcg ctcatctttt
    27001 actctgattc cagtgattaa cgccttgcca aaaagatcat agagtacctt tctaaccatg
    27061 ttgattctaa ggccagtcga gcttgcaatc tcttcgtctg ttgcgtcttc tgcctttagg
    27121 agtgcgcgtg ccaccttgag atattcgtct cctccaatca tagcggcgat cctaacaaaa
    27181 ggatcttcgt aagattcgac caatgttatt cagtttccta ggtccatatt tattcaatcg
    27241 ttttttgcga ctactttttt gccctttttt gtcggaacaa tctcaagttt tgcattgttg
    27301 tattttgcat caaactgttt tccaccctga attctgtcaa gcaagactgc aagcgcacta
    27361 atttcagagt gaggttgatt tccaacagct acattgtaat ctgcttgctc gtacacatcg
    27421 cgtggaacct tttcagcacc tacaacaatt aggacgttgt cttcttgtgt aattttttgt
    27481 tcaacatcat tgatgttttc accatacatg gtaagatgca cgattttggc aacttttttc
    27541 tttgatctta agagttttct ccagtcctca atgaactcga tttgaaaatc gctaccccaa
    27601 gatttgttca ctttgtttag tgtatctttg atttctggat ttacctcatt catgtaaatc
    27661 tttgatgcgc caaatgcacg tgacaccaat gccacatgcg ttgtcaccct atcgtctcgg
    27721 accagccgtt gccctattct taacacttca atcttcttca tagaattctg ccagatctga
    27781 tttttttgga atttctttta tttgctgcgc tggtagctct agcatttttt tgacaagatc
    27841 caaaagcttt gggacgtttt gtttcgttac tgaggaaatt ggcacccatt ttttgctgtt
    27901 ttcaaagccc aacagctctg atttttgcac aatctcttca ggacttgcaa ggtcagattt
    27961 gttgagagcg tagattatct tttcagaatc tacgtctagg tctgctagtg ttttgaggca
    28021 gcttcggaat ttctttttga gttcttgggc agaatcagaa atatcaacta caacaattac
    28081 aacgtcggcg tatcgaagct cttcaagtgt ggacttgaac gcttcaatca tgtaggcagg
    28141 aagcttgctt atgaatccaa cggtatctga aaccaaaact gggttttttt caatgaaaat
    28201 ttttcttgtg gtagtagaaa gggtcgtaaa taattccgga ctttcttctt ttgtttcacc
    28261 cgtcaataaa ttaaacaaag ttgtcttacc ggcagacgtg tatcctgcaa gcgaaactgt
    28321 tctcagccca acccgccttc gagcctctct gtgcaatgct ctttgttttc cagtttttgc
    28381 aagcttagac ttgacacttc tcattctgtt ctggatatcg ttatagtacg tgtcaacttc
    28441 gtacattcca attcccataa agcctggctg ttcacccatc tttgcaagcc gaaccttttc
    28501 ttttgctcgt gacatctcat atcgtagctg ggctagcttg atctgaaggt gtgactcaaa
    28561 gcttgatgac cttttctcaa aaatctccaa aatcaatgac tctctatcta aaatgtttgt
    28621 ctttagcgta gatgcaagat tgtagctttg acttggtttg agtacctcat caaaaactat
    28681 gacatcgggt cttatctttg tgacaagctc tttgatctct agtacctttc cctcgcctat
    28741 tccatacttg tgtttatcaa gaaccttttg cctgattatt tttgcaacct tgtagcccgc
    28801 agaatcgcag agcccgagcg cttcctttat ggcatcttcg ttgtcatatg tgattaagat
    28861 tgcagtttgt gtcaatacaa tcagtttttt gtctttttca gcatcttttc tatgttcata
    28921 tttagtacga tttccgcaat atcgcttgcg tattccgcaa ttcggatgat attttccgac
    28981 attcttctta ttctgtaggc ttcttcctcg tcttttatgg acttgatgga ttctaggatt
    29041 cgcttttcat attttgaagt ctctgcggcc tttttgattg caagttctgc ctggacaaag
    29101 tcttctttga acaacgcaag acatgcttga tccaaaaccg aggttgcaaa gttgttgagg
    29161 tcctcaattt tttccataac ttcctttctt acagattttt tgaactccaa aagatccttt
    29221 gcaattaaaa ctgcatgatc accggttctc tcaatgttct taactattaa ccgataaccg
    29281 aggcactgtc ttgcatttgt aaatcccata tcttttaaca tatactcgtt tgaaattgca
    29341 atttttagct gtctgattat atagaaacca aatctgtcta cttcatcatc actgtcaatt
    29401 acttctcgtg ccaaatctag atttgcctcc tcgactgcaa gcaatgcatc ctttaacatt
    29461 gattttgcaa ggtgcaacat tcttttgaat gcgccatcta ctgtcagctc aagtagattc
    29521 acaagaacct gtattgttat tccttcaaca gaatctgaaa ttatttcaga gcccatcaac
    29581 attcctttta ctgcagattt gatagaattg cgttggcccg gttggagtct atctgtcttt
    29641 ggttttatct gaattgtctt gtacccaaga aaataaagtg acacaagctt tcttacaatt
    29701 gcggcgttat cgtcatcacg tgttatttct aaaactgctt cgtctacttg tgttgatttt
    29761 gcctgatctt ttgctggtaa gattttcaga gccgattttc cttcccgagt tatcgtaatt
    29821 tggtctcctt gttttagacc aagctccaaa acccattgtt ttggaagtga aacaatatac
    29881 gatgattttc ctgtaaattg gagccgcctt gtctcttctc gttcttgcat actggtctgg
    29941 gaatcattgt tctatatagt ttgtttgctg tctatgtgac aacacgtttt ttcatattta
    30001 atggaaagag gttattttca gggctgataa tagttaccac cattaaacat aacttcaaca
    30061 taattaattt tgaaaaattg gcaaaagatc ctgtctgtgg aatgattgtt gatgagaaaa
    30121 gggcactaaa ttctgagctg ggcagcaaga cattttattt ttgcagccca gtctgccaaa
    30181 agacattcct tgagccagaa aaagagcttg ccaaaatgaa aaagcggatg tatcttgccg
    30241 cctctggagc tttggccttg gcaatcataa gggggggcgc ttacttggcg ttagctgccg
    30301 gagccattgc ttttacatgg gtcccatttc cagagattcc gtttctgtca tatggattac
    30361 ttttattttt tatcgtaaca ccagtgcagt ttatcggagg ttggtcgttt tatgttggag
    30421 gatatcatgc aataaaacgt cgtactgcaa acatggattt gcttatttca attggaactc
    30481 ttacagccta catctatagc accgtggtgt tgttcttccc agatgtaata ccaactgaag
    30541 aaaaatacgt ctactttgaa gtttctgcag tcatcatagc cttcgtgctt cttggcaagt
    30601 acatggaaga ggcaatcaaa aagaaaagct cagcatctgt gaggaagctg ttggacctaa
    30661 gaccagcgat ggcaacggtg gtcagggatg gaaacgagat ccaaattccg gcaaatcagg
    30721 ttgagctagg agacataatg attgtaaagc caggtgaaaa aattccaact gacggattga
    30781 tcgtcgatgg agagtcatca gttgatgaga agatgataac aggagagagt ctgcctgttg
    30841 gaaaaacaaa aggggacgaa gtgattggtg caacattaaa caagcatggt ctactcaagg
    30901 tcaaggccac aaaggttgga aaggagactg cactttctca aattattcac attgtagagc
    30961 aggcacaatc atcaactgca aaggtccagc gtctagcaga ttccatttca gccaaatttg
    31021 taccggccgt tgtgattact gctgttacaa cgtttgtggt atggacgttt ttgctaagtg
    31081 accttgtttc aggcatactt acctttgttg cagttttgat tgttgcctgt ccatgtgctc
    31141 ttggggtggc aacacctgcg gccctcatgg taggggttgg aaagggtgcg gagtctggca
    31201 tactaatccg aggtgcggaa taccttgagc gctctcaaaa agtcaatact gtggtattag
    31261 ataaaaccgg aactctgact aagggtgagc ccgaggtgac tgatgtcgtg aatttgggaa
    31321 ccctttcaga agaaaaacta gttgaatttg caggcgcagt cgaatctggc tctgagcatc
    31381 caatagcgca ggctgttgta aaaaaggcaa aagaaagcgg aattttagtt cctgctttgg
    31441 aaaaatttga agtgcttaac ggtttgggag taaaagcaat agtaaggggg aatttcgtct
    31501 atgctggaaa tagaaagatg atgaaaaagt ttggcattcc aactgaacca gtagaagaaa
    31561 agatgtcaat gctggaatcg cagggaaaaa cagcaattat tgttgccgtt gatgacaaaa
    31621 ttgaaggcat cattgcagta gcagactctc tgaaggaatc ctcgcccatg gcagttgcag
    31681 ccttaaaaga tcttggtata gaatgcatca tgataactgg agacaatgaa aagacagcca
    31741 agacaattgc agaaaaagta ggtattgaca aagtcattgc aaacgttctt ccctccgaca
    31801 aggcatcgga aatcaaaaag ctgcaatcac agggcaaggt ggtcgccatg gtgggtgatg
    31861 ggataaacga tgcgccagca ctggcacagg cagacattgg catagcaatt ggaagcggct
    31921 ctgatattgc caaagagacg ggaggaatta ttttgataag ggatgatgtc atggatgtct
    31981 caagggccat ccgccttagc agggtaacta tgaaaaagat aaagcagaat ttattctggg
    32041 catttgcgta caactctggt gcaatcccaa tagctgccat agggctgctc agcccaatac
    32101 ttgcagctgc tgcaatggct ctgagttcca tttctgttat tgccaattct tctttactaa
    32161 agcgctacaa gattaccagc ggtgaagaaa aactttcttt taaaaaagaa tacagccaag
    32221 aaaaaccaat tgaactaata cactaatcaa aacccttctc tataatttta aaaatggcgt
    32281 atcacacaga aattttcaca tattaataac aggtaaattg ttaagcattt gatggaccca
    32341 atccttaagc taaagtacac aattgatgcg ctttttggtg caggcgtctc aaaacatctc
    32401 ccaaaggaca tcacaattac attttcaaaa aagactggaa ggattcgtga ggtaaagcac
    32461 aacggcagac tcttgtgcac gcttagaatt gacggcggtc ttgcaataac cacgtatttt
    32521 gcacaaatcc tgctaaaaag taaaaaattc cgtcaaaact gcatcgaagt tgacgaggac
    32581 tctaaaccgt ttgtggaaga tggcaaatcg gttttttgca agcatgttac ttgggctgga
    32641 aaaaacattc tgattggtgc tgacgttcca gtactatacc aaaataaagt aattgcagta
    32701 ggtcgggcaa ttgtaaacac agaaatgatg atgtacctaa aacgcggagt tgcagtcaaa
    32761 gtcagagata gtttaaaatc tagtttggag gcataaacaa tcatgatgcg aggcggcaac
    32821 cgccagatgc gaagaatgtt ggacaaaatg ggccttgata tgaaagagct cccaaacgtc
    32881 caagaagtaa taataaagac agacaaaaaa gaaatcataa tttccaagcc ttccgttact
    32941 gagatgaagt ctcaggaaaa ttcaatcttt caggttatcg caaatgggta tgaggagcgc
    33001 gagctagaaa ccccagtgtt ttcagaagag gacattatgc ttgtgtccca gcaggcaaat
    33061 gtaactccag aagttgcagc aaatgcccta aaggagacaa acggcgatct tgcgcgggca
    33121 atccttcttt tgagtacaaa atgaaggctt taattatgga aaaaggggat ttttaactga
    33181 tgaaggaagc agcacagaca gcagtcgcgg attctaaggt cagcaaaatt atcaagtcct
    33241 tggctgatct tgagaacgac attgattccc aaaacatcaa gattgccgaa atgaaaaaat
    33301 cattaaattc aaaggctcta aaggagatag attcactaaa ggaaaaggta actcagacag
    33361 caataaaaga agccgaatca atgatttctg aaaccaaggc aaaagcagaa cagcaagcaa
    33421 aaaagattgc ttcagacgga gcagcaaagc ttgacaaact caaatccaca attgattcaa
    33481 aattcgatga ggcagttgac agcgtagttt ctacaattct aaaaccctaa attttcaaac
    33541 cctaaagcct taaattcgca aaaaccatca atgaaaggta tcagcacatt agaagattgt
    33601 agaattggaa ttgccaccac ctacggcaaa ccttatttcc aattcgtcaa ggcactcaag
    33661 tttctgaacc taaaatatga ctcacttttg ccaacggaga tccaaagcta tccaggaagc
    33721 ctcattttga caacgcagga agaatcgcct caatctgcaa gccagacgat cctaaacgag
    33781 gagatactaa aggaagaccc aaccgtaatt cagggaataa taatcgaaaa acttgactct
    33841 ggtctaaacc aacacgagct aataattgga atcgacccag gacagagaat cgggctttct
    33901 gtactatatc ttggaaagga aatcgagcga ggactgtaca cctcaccaga cgagcttgcc
    33961 tttcatgtgg caaggattct tggcggactc cgatcagaaa aaaagattgt caaaatagga
    34021 aatggaaaca tggatgttgc aaaaaagata acaacccttc ttaatttgag gttctgctct
    34081 cactttgagt tagagtttgt tgatgagaga aaaactacaa gaaaaatcaa aaactacaac
    34141 aagcgcggcg aacgcgatat gatgtctgca aaatacatca cacaacgcca agggtatcgt
    34201 cgttttgttc tacccctttc aagaactgga taatttcgta aaatccgcaa aaaatgacca
    34261 aaaaataggc aaaaaaatcc caaaaaccgc gcctgagggg tttaatatac catgtgtaaa
    34321 aaaatcgtaa gatatttata catcttgacg tggttttttc aaattgatcg agctattccc
    34381 caaggttcaa taaaaaaatt acaagatttt caaaataaaa ctatagaaat tttagatcca
    34441 tccttaaata tttgcgaatc ctatatcata ttgaagaaat atgacatgta agggaatctg
    34501 ctccagatac aaggctcaaa agccagtagg aaccggacgt tatgcttcag gacagagacg
    34561 ttgtcaaatt tgtgaaatct tcatcaaatg ggaaggactt tggtgtccat gctgcggtta
    34621 cagactgaga acaaaaccac gaaatctcaa gtacaaagcc aagctgcgag ccagagttga
    34681 agctgaagct caagaagcag aagcaatagc aataaaggca taagtcttac ctagcaatta
    34741 atgtcaagtt ctgaccacag agtaatctct gtggaaacag aaaatcggaa attcgatgta
    34801 acaatcgtaa agtacgataa tggttttttc atttccatca ccgaaggtgc tagaaaattt
    34861 ggtgcaacta taatatcaat tggaacgggt cctgcaccaa gctcatctat tattattcca
    34921 gcaaaagccg actcgctttt tttgaaatta atttctcaaa aggtagcatc agaaattagg
    34981 ggtttgtgta ttgcatcatt gtccacacaa aaagaacttg gagcaaacgc cggcaaggtt
    35041 ctaattgcca agataatgga ggcagtaaga gaatgacaga cattcgtaat ttcattgatc
    35101 atataaaaaa atctggacaa ctctcgataa taaaaaagaa agtatctaca aaatatgaga
    35161 ttgcagcaat tactgccaaa gtcgatggcg gcaacgcaat tttatttgag aacattaaag
    35221 aaagcaagct gcgcctcgtt gcaaacctgg ttggaacacg tgcaagattt gcaaaggcaa
    35281 ttggagcaag cgagtcaaca attcatcaca aagtaatttc ggcaataggg catgcaaaaa
    35341 agcctaaaat tgtatctggt ggaaaattcc tcgaaaacag ctcacgggat ttgtcagttt
    35401 tgccagttgt tacccatttt gaaaaagagc caggaccgtt tatcacatct tcaatcattt
    35461 atgcaaaaaa tccggaaact ggggcacaaa actcttcatt ccatagactg atgccgcttg
    35521 ataagacaca cttttctgtt aggatggtag aaggacgaca ccttcatcgc acctttgttg
    35581 acgcaaaaga acatggcaaa gacctcaaag tagcgataac tgtgggcgtt catcctacca
    35641 tatccattgc aggggcatat caggcagaat ggggaaaaga cgagctagac atcgcaaaca
    35701 cgcttttggc aggcaaactt tcccttacaa aactgccgta ttctggcctt gccgtaccaa
    35761 gtggtgcaga aatagtcatg gagggcaata tcttagcaga caagacccac aaggaatgga
    35821 tggttgagat gctaaggacg tatgactttg caagacagca gccagtattt gagctcaaaa
    35881 agctgtatta cagaaacaat gcaatatttc atgacatact atcaggatat gcagagcaca
    35941 ggctactgat gggaatgcca attgaggcaa aactaaaccg cgagctcaaa aagtcagttc
    36001 cacaaacaaa ccaagtggtc atgagtgatg gcggctgcaa ctggcttcat gcagttgtac
    36061 agatttcaaa gaaaaaagat tctgatgcaa aaaaagcaat cgaaaaaacc tttgcctcgc
    36121 atcgctcgct aaagctagtc acagtagtag atgacgatat tgacccctcc gatcctgtgg
    36181 ccgtagaata tgccatggca acaaggttcc aggcagacaa agacttgaca ataatacaaa
    36241 aggtaagagg atcaagcctt gatccgtcaa gtgatcaaaa gaatctattg actgcaaagc
    36301 ttggaattga tgctacgcgt cctctttcaa aaagaaaaga aggatttgag attgccaaga
    36361 ttccaggcca agaaaaactt tcactaaaaa aatatctgaa atgagccaag cttttttacc
    36421 taaaatgggg tttgatataa catggaagta aaaaggcgca gcagttggtg gttcctctta
    36481 ccaattctct ttagtgtaat aggtggcata attgcctact ttgtgatcag agaagacgat
    36541 ccaaaaaaag caaaggactg cttacttctt ggggcaatac ttactgccat cggaattgcg
    36601 atatttgtta tcccaatttt ggttggaatg tcgtttatgc cacacactaa catgtttgag
    36661 agtagcttct aagtttagga tttctaaaga ggcatgttta ggctttgaat tttcttgatt
    36721 atttcatcat ggtctgcttt tacatcaatt gagaaaatga gcagcccgtc atccaaaaac
    36781 aatgtgatca gctttacctt ttcctttgca gttatcatcc agtttgtctt gcctagtgca
    36841 tcatcgaaca tctcctccat acttgctttt actgatgcgt ggtaatgagc caagtttcct
    36901 aattcagaat ccaaaagcgg ttttttttct ggatgtcgtg caaaggcaat tacctttcta
    36961 tccttgattc ttccaacaaa tcgtatggaa tcatcaaggc tcaaaacctg tttgcatata
    37021 tcaagtggat ttagctgttt atgcactagt atccccttaa gcaacaaacc tcagaatact
    37081 tgttaaaagt ctttttattg atgcagaaaa tcgaatgtaa ttttacaaat ttttagctga
    37141 tctgctatat ttttccaaat ttttcagttt gatgatatca aattttagat tctaaacaat
    37201 ttacatatgc ccattgtaag atctggaaaa ttgcttttat gtgattataa atctgatcaa
    37261 actcaatgaa tgataaactc aaactagctt cgttgctgtt gatactacct ctctttactg
    37321 ttgggctaac aaccaacttt caagatgctg aggcgttaaa aagcaaggga actcctctat
    37381 cggctacatc atcaagtaag gtctgtggag acaaactttg ttctgagatt ccaggcggaa
    37441 aatctgcaat ggaagaaaaa ctatccaaaa aaacagaaaa gatgacagaa aaggaaacca
    37501 tgatggaaaa aaattctatg gtggaaacgg aaaccatgat ggaagaacca gggtatgaac
    37561 taagacttgc aaggacaaat gttcctgtga cgatcccact tcatcagggt tggtacaatg
    37621 gtgaatcagt gtactttatc ataaccgatt ccagcgaaca aaagcatgcc gatgttatca
    37681 cacaaaatca gggttggaaa gtagagcttg ccccattttt ggcaaatgcg ccaaagaaag
    37741 cactttcaac aacatacgtc tttaccaacg gaattgaagg tggtggaatc cacggattcc
    37801 aaagcgaggt ctttacaaac actcctgccc agccagaaac gtacagcgca ctgacatcac
    37861 acgtccatgt aacatggaat gatgcttcac aggctgatct tttggattct gaagccgcaa
    37921 tacttgcggc agaagaggaa ggcttggtga cactaagtga tcttccagtg gtgattaaca
    37981 tgccacaagt tgtgtggcct ggaggacagc tccaagttaa ggacgacaag acattaaccg
    38041 atgacacacc atacggcgga ggacaggtac tagacattga cacagacgaa atgacagtca
    38101 catttgtagc acacagaggg tggggaccag acggtagaac catctactat attgtcacgg
    38161 atgcaactcc atcaggccca gctgccatga tgggcgtagc aagctcacca acttcagctg
    38221 cactgattgc aaattctgca gcagtagact tgttccaatt catgaatggc gccaaaggaa
    38281 ctggcccatt gggattccag ccaggaattg cagcagctgc tcctggagat aagaactatt
    38341 ctccaatgtg gagaatattt gcgattggat ggaaggatcc gacttctgca tcagtacttg
    38401 aaacagtagg tgatatcaac gcatacagca aagcaggatt gattgatgtg aatttggcgc
    38461 gaccaatgga tagtgatcac atagtgaact gtccgttcat cgatccattc caataacttt
    38521 ttttattttt tttgagtaac aacaatttgg tgttaaattg aatcaatttc agataaaatg
    38581 tgcagattgt gagtgcttgc catcagaatg caaaaaaagt aattcaccag ttgactgccc
    38641 aaattgcatg tatgttgagt gttgttgctg gactgaaatt aatcaacctt cgtagttttc
    38701 ttaattctgt tataaataaa ataaaacgca gccccactca aaacaactcc aatacccatc
    38761 aatatcacat atacatccaa ttgaatcact ccaacaattg gtgtgattat tccaagtaca
    38821 ggtaaaattg gaaagttttt cacatttagc ggaactttga atggcctttc agcttgcggc
    38881 tcctttaatc gtagccaaat caacgaaaaa ttcactgcaa tgaaaaccat gatgatggca
    38941 aacacagaga tatttgctat agtcgaaata tctcctgcaa aggtaaatgc aacagctaga
    39001 gaaccaatca taattactcc agcccatgga gtttttctcc cctgatgaat aattccaaaa
    39061 attgctggaa gcgatttgtc acgagcaatc ccataaagaa ttcgtgatcc cgaaagcaac
    39121 atgattaaaa ccgtgtttgt tgtagcaaaa agcgcaatta acaaaagaat gatctgggcc
    39181 tgggggccaa gtgctttttt tacaacatcc gcaagaggtg caatcgaaga cccaagttct
    39241 tgccagtcta gtaccctgat tgcagaaatt gatactagaa tgtagattat tgcagtaatt
    39301 gatatggaaa gaataattgc tcgcggaaga actttttttg gattacgtat ttcttcagca
    39361 atatttgcaa tgttttcaaa gccaatatag gcaaaaaaca caagcgtaaa gccagaaaat
    39421 atgcccatta gaccatgagg tgcctcaaaa taatcaactt gtgaattccc aaacaacgca
    39481 aaaccaatcc aaattatcaa tcctagtcca gcggcttcaa ttatggtaaa aattacattc
    39541 atagaagatg attcccgtat tccaataaaa ttaacaaatg agagaaccac aattacaagt
    39601 attgcagaaa tcaaaattgg tgcgccaaac aaaccagcaa aatatccacc aaaaccaagt
    39661 gcaatagttg atgcagaaat tattgcaaca aacagtgtaa gccaaccaac aagaaatgcc
    39721 acaaaattat tcttgaatgc atttttggtg taagaatatt cggcagcagc ctttggatac
    39781 atagaagaaa gctccgcata acttagtcct gtaaacacag cagccatggc agccaaaata
    39841 aatgagaccc aaactgaatt tcccgcttct ccagtagcac ttccaattaa cgcataaatt
    39901 cccgcaccaa ggataagacc aacgccatac atggttgtct gaaaaagccc aagatgacgt
    39961 cgtaattctg acatgaatta tcctacaact ttggaattat ttatctgcac aatggaaccc
    40021 aattctgata attatcactt ctatacttat acgataaaac agctaaattc tttgtgaaca
    40081 gtgataagaa attcataata atcgggctga ttggtggttt tattgcactt gctgcaattt
    40141 ttggttatgc aagccaagga tatgtcagac cgttcaatat tttttggcca gaagaagtca
    40201 ggcaaacatt agagtttcga actctggatg ggagaacagt tgtagtaggt acggtaggaa
    40261 acgccggaac aaatccagaa cttgtgatga gaactggtga ttattcttac attattacgg
    40321 ttgtaaacaa ggatgtgata tcacaccagc tattcgttga aggacctaat attagcacaa
    40381 aattgttaga accagatgaa cgtgataaaa taattttaca aagcaaaaac gaggcaatct
    40441 tcaattatta tgataccgca aatggtaaag aatttcttgg aacaattcgt gcagttaaag
    40501 taacgccatt tgaataatta aaatatgatc ttaaaactga gtgaaaaatc aaattgatct
    40561 caataattgg tagcggcaaa gtaggatctg cacttgcatt tctttgcggc tcttttgcac
    40621 ttgatgatgt gattctggta aatcgtaacg agaaaaaagc aattggtgag gcattagatg
    40681 catcaactac aattccagca acatcgacaa tatcaattaa tggaacaagc gattattcga
    40741 aaatcaaaaa ctcagatgtc gtagcaatta ccgcaagcag cggagtacat ctgcaaagtc
    40801 gaaccgaaat aatgtatgat caggccatta tgattagaga aatctcacaa aatatagcta
    40861 gatacgcacc tgaagccaaa atactgatga taacaaaccc cgtagatgtt cttacatatg
    40921 taatgcaaaa ggagagagac tttccatcaa aaaacgtaat aggtgttgcc tcaagccttg
    40981 actctagtag atttagatat cttttatcaa aagaatttgg tacaaaccaa tcaaaaatta
    41041 ctgatgccct agttttggga gaacacgatg attcaatggt cccaattttc tcatacgcaa
    41101 aattcaatgg aaaaccagtt caagacattt tggatgggca aaagaaatcc gagattacaa
    41161 atgaggtaag aaattattgg aaataccttc gagattacaa aggacattca gtgtttggta
    41221 ttgcaaaatc tacctttgat ataataaaat gcataataaa aaatgaaaca ttggatattc
    41281 ctgcctcgac attacttgat ggacaatttg ggctttcgga tgtgtgtatt ggtgttccac
    41341 tcaccataaa caaaaatgga attgcaaaaa ttcatcaaat caaaatcacg aaaaatgaaa
    41401 atgaatccct gcagaaatct gcacatgttg taagaaacaa cataacaaaa gttttggaat
    41461 ttttaaaaac taactaatga cttgtcttgc aaggtttcgt aatattatta tccgtttaat
    41521 ggaaatgcca gttttgttta caacctcatt tacgggcata tctaccaaat ctttggcaat
    41581 catcagactg tttttggaaa ataacactaa ttccttttta gttggaccca gtatggtgat
    41641 gggatacagc ttcttttctt caatcattcg ttccaaaccc ttttcggttg gatacttcca
    41701 agacaagact tgttgcccaa cacattttgc gtatgtaatt acatctgaag ataccttggt
    41761 attgcaaaca agcatttcac catcaaattt gtcagatagg tccaaaaatc gtgcatgcgt
    41821 atacagggat tctttgagtc ctgtgtatct gccaggcatg ttatgatatt tgcattcggc
    41881 catccacttt tttccgtctt gaattgattc caagacgagg tcgacttcat gcttgacaca
    41941 tcttcctgtc atctcggtac gaatagactt ggtagtgtaa ccaaagtgtc caagaatctg
    42001 tcctacgtag atctcaaatg aaaacccagc aggtcccatt ctcattattg cttcttttag
    42061 gcgatacctg tgtttgatag ctatcatccc atgttcggaa agaaaatgca gtaccattct
    42121 gtaaatttct ctagttgaca ttccctggcg agttttagag taaacctgat tcgcaattct
    42181 ttttgctaaa tcaggacttg cacctgctct gatacatgtt gattctactt tgtttggatc
    42241 aaatggtgct ttttccccat ttgcttttat gatcattatt tttttcaata atttagtaca
    42301 tcccagatga ttgattaagc attttgccca gctacagtac aactggaagt aatgttctca
    42361 atgattttgg ctgtgttgat gttttttggt cgtctctact tcttgattgt tttaggctct
    42421 gagaatttga attttagagt tatcaaggtt gaaaattgac cattaattgt atatagatat
    42481 aattacgaat caaatcagtg acaaatcaaa ttatttggat aggagttgta gtgggtgtct
    42541 tctttgcagg actaggcata ggatatgcaa tgttttactc gactcaacca aactttacgc
    42601 acatgactcc acaacaaatg caacaaatga tgaacaatcc tcaatccatt caacaaatgc
    42661 atgaccttat gatgaatgat ccaaaccaca tgcaacaaat gcatcaaatg atgatgcaaa
    42721 gtccacaaca catgaataca atgatgggta caatgatgca aaattcccag tttggaagcg
    42781 tgatgatggg acaaatgatg aacaacccaa acaccatgaa cggcatggtg aatcaaatga
    42841 tgaacaaccc acagttcatg aatacgatga tgggtacaat gatgcaaaac caaaactttc
    42901 agcagcagta catggggcca tggatgatga tgagggactc acagtttatg cataatatga
    42961 gaggccaatg gttccaagag acaaaccttg agagctcggc agttcaaaca aaccaagttt
    43021 ctatactggc agacacttgg cgataccagt ccacaaaagc gtactctcca tccgtgataa
    43081 aggtctcaac tggcactacg gtcacatgga caaatgatga cactataatt cacacggtaa
    43141 cggatcttgg aaacgagttc gattctggat tcattcaggc aggtgagact tggcagtaca
    43201 cctttgatac aaaagacaca taccattact tctgcagcat ccacccatgg atgagaggca
    43261 ctgtattagt gtcctaaaac caacttttta ttttcaaact tgattttttc ccagcaattt
    43321 ttaatctcac tatgtggctt taataacaat gaaatggcat tttgaggatt tcatttatgg
    43381 ttcatttgac ggctcggtta ccacatttgc tgttgtagca ggagcaatag gtgcttcact
    43441 ttctcccatg attgtggtga tacttggatt tgcgaacctt tttgccgatg gttttgctat
    43501 ggcagtaggt agctatcagg caaccaaggc tcgaattgag ttcattcaaa aggaaagaaa
    43561 aagggaggaa tgggaaatcg ataatatgga agaatcagaa aaacaggaaa tccgtgatat
    43621 ttatgcaaaa aagggattca ctaaagagct cttggatgaa attgtcagag taattactgc
    43681 aagaaaaaaa gtctgggttg atactatgat gaaagaagaa ttaggactaa tcgaagacga
    43741 taaaaaacct cttgccagtg ctacaagcac aattatgggt tttaccttaa ttggcattat
    43801 tccactaatc ccatttgtaa taatctatgc attgggactg acaagctcag tttctgaaag
    43861 ctttacatct tcagttatct ttacaggcat cgcatttttt ataattggaa taatcaaagg
    43921 aaaaattgtc aaaaaaccat tatttcaatc aggtcttata acattattaa ttggaggaat
    43981 tgcagcaacg gttgcatatc tggttgggaa cttgataagt aatcttgtta gaggctagta
    44041 gctggctatt gcctcttcgt tatggtcatc aaatattttt gtcagcgcgt gataatacat
    44101 ttttcttgta tcctctctat tacatacagt atacaatttg atcaaatcag tagttgtgac
    44161 aatccctagc aatttgtcct catctaggac aggaagtctt cgtatctttc ttgaatacat
    44221 caggtctgct gcaatccata cctcatcgtc tggacttaca tgaatcaaag gtgatgacat
    44281 tacctgcttt accttggtgt gaattggata tgcatgagca gcaattttta ttgcaaaatc
    44341 cctgtctgtc attattccaa ctggaatgtt ttgttctaaa actaggacag agccaactct
    44401 tgccttttcc atcattttag ctgcctcatt aacggttgca gacgaat
//
LOCUS       DASWKH010000118         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3263756, whole genome shotgun sequence.
ACCESSION   DASWKH010000118 DASWKH010000000
VERSION     DASWKH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263756"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VGA92_08935"
     CDS             <1..437
                     /locus_tag="VGA92_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846566.1"
                     /translation="IGVIAGIVFFGALMTIIGQGTVRHVDIFYPETVRQTVAFRDDGI
                     KTTVDGIVGNGEKNNPTLITRLGFMYILTVINDGTSPHRLYIDGLDIQTELLASGEKD
                     IITFTPQSEGVYNYYDKQQELLLGQLKVVEVIQIDAIEANSG"
     gene            509..2152
                     /gene="thsB"
                     /locus_tag="VGA92_08940"
     CDS             509..2152
                     /gene="thsB"
                     /locus_tag="VGA92_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX9846567.1"
                     /translation="MAQLQTTAEGIPVILLKEGTRQSRGRDAQRNNISAAKLIAEMIS
                     TSLGPRGMDKMLVDSLGDITITNDGATILKEIDVQHPAAKMMVEVAKATDSEVGDGTT
                     SAVVMAGALLEKAESLIDNEIHPVIIADGYKKASRKASELLLEIAQKVSVDDRSTLEK
                     IAKTAMQTKLVSIEASDLAKLVVEACLAIREEKNGEYKIKIDNIKVEKKAGGSVSDSQ
                     IVNGIVLDKEVANSGMPRRIENAKIALISAPLEIEKTEFEAKINISDPNQIKSFMDEE
                     DKILRDMVEKIKSANANVILCQKGIDDIVQHYLSKANILAVRRIKESDMSKLAKATGA
                     RIVGNVNDLSEKDLGLAQNVEEKQIENDNWVFVEGCKNPKAVTVLIRGGSQRVIDEAE
                     RSIHDALMVVKDVVERPLMLVGGGAPEAYVAVKLRTWSQSLSGREQLAVEKFAEALES
                     IPLALARNAGMNPIDAITQLRAKQSAGEKYTGIDVINGTVGNISKLDVLEPLKVKEQV
                     IKSATETANMILRIDNVVAVSKSASPPQGMPPGGGMGEY"
     gene            complement(2173..>3032)
                     /locus_tag="VGA92_08945"
     CDS             complement(2173..>3032)
                     /locus_tag="VGA92_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEX9846568.1"
                     /translation="AFLIAATSLDDIVLVNRNKEKALGTALDISNSVSVDSPVSVVGT
                     DDYSKIKNSKVVIISASAGNSGTSRMDFLEQNVVLIKDIAKKIKQHSPESKILIVTNP
                     LDVITYVFQKEVPHSKEKVIGIAGNLDSSRFRYILAKELNTDVDKITNALVLGEHGDT
                     MVPIFSHARYNGKPVLDFLSTTQVQQITDNVRNYWKDLRKYKVFSIFGIAKNTTDVAR
                     AILKDEKLSLSASVLVDGQYDLSDLCLGVPIKINKNGIDKINEIDLLKSELESLHHSA
                     LVVKNYLAA"
BASE COUNT         1050 a          512 c          612 g          858 t
ORIGIN      
        1 tcattggagt cattgcaggc attgtatttt ttggtgcgtt aatgaccata ataggacagg
       61 gcacagtaag gcatgtagat attttctatc ctgaaacggt tcggcaaact gtagcgttta
      121 gagacgatgg tataaagaca actgtagacg gaatagtcgg aaacggtgaa aaaaacaatc
      181 ccactttgat tactcgtctt ggctttatgt atattctaac tgtaatcaat gatggtacat
      241 cccctcacag gctatacatt gatgggttag atatccagac agaactttta gcatctggag
      301 aaaaagacat tattacattt actccgcaat ctgaaggagt ttacaattac tatgataagc
      361 agcaagagct attactagga cagttaaagg tagtcgaggt aattcaaatt gacgctattg
      421 aggcaaatag tggctagaat ttttaaaacg attatcaatt gtggaatttg taatcatccc
      481 ataaatagaa aatctttgtg ttatttttat ggctcaacta cagactaccg ccgaagggat
      541 tccagttatt ttactaaaag aaggaactcg ccaatcaaga ggacgtgatg ctcaaagaaa
      601 caacatatct gcagccaaac tgattgcaga aatgataagc actagtttag gaccgcgtgg
      661 aatggacaag atgcttgtag attcacttgg cgatattaca atcacaaacg atggtgctac
      721 aatactcaaa gaaattgatg ttcagcaccc agctgcaaaa atgatggtag aggttgcaaa
      781 agcaacagat agtgaagtgg gcgatgggac cacatctgcg gtagtcatgg caggcgcact
      841 cttagaaaaa gcagagtcat taatagataa tgagattcat cctgtaataa ttgcagatgg
      901 ctacaaaaag gcatcaagaa aagcaagcga gcttctttta gaaatagccc aaaaagtaag
      961 tgttgatgat cgaagtactt tagaaaaaat tgcaaaaact gcgatgcaga caaagcttgt
     1021 ttcaattgaa gcatcagatc tggcaaagct tgttgtcgaa gcctgtcttg caatacgtga
     1081 ggaaaaaaat ggcgaatata aaataaaaat agacaatatc aaggtagaaa agaaagctgg
     1141 tggttctgtt tctgacagtc agattgtaaa tggaatcgta cttgataaag aagttgccaa
     1201 cagtggcatg ccaagaagaa tagaaaatgc aaaaattgct ttgattagcg cgccacttga
     1261 gattgaaaaa acagaatttg aagcaaaaat aaacatctca gatcccaacc aaatcaaatc
     1321 tttcatggac gaagaagaca agatcttacg tgatatggtt gaaaaaatca aatctgctaa
     1381 tgctaatgtg atcttgtgcc aaaaaggaat tgacgatatc gtacagcatt atctttccaa
     1441 agccaacatt cttgcggtaa ggagaataaa agaaagcgac atgtcaaagc ttgcaaaggc
     1501 tacaggcgca agaatagttg gaaatgtaaa tgatttatca gaaaaggatc ttggactagc
     1561 ccaaaatgta gaggaaaagc aaattgaaaa tgacaattgg gtgtttgtag agggctgtaa
     1621 aaatccaaaa gcagtaaccg tacttatacg aggaggttca caaagggtaa ttgatgaggc
     1681 tgagcgctcc atacatgatg ccttaatggt tgtaaaagat gttgttgaaa gaccgttaat
     1741 gcttgtaggt ggcggagcac ctgaagcata tgttgctgta aagcttagga catggtcgca
     1801 atcgttatct ggtcgtgagc aattagcagt tgaaaaattt gctgaagctt tagaatcaat
     1861 tccccttgca ctagcaagaa atgcaggcat gaatccaatt gatgctataa ctcagcttcg
     1921 tgcaaaacaa tcagctggcg aaaaatatac aggtattgat gtaattaacg gtaccgtagg
     1981 aaacatttca aaacttgatg ttctagaacc actcaaagta aaagaacagg taataaaatc
     2041 tgcaacagaa actgcaaaca tgattctaag gatagacaat gttgttgcgg tatcaaagtc
     2101 tgcttctcca ccacaaggaa tgccaccagg tggaggaatg ggcgagtatt aaaattaata
     2161 tttttagaca attcaagctg caagatagtt ttttacaact aatgcagaat ggtgtaatga
     2221 ttctaattct gattttaaaa gatcaatttc attgatttta tctataccat ttttgtttat
     2281 ttttattgga acaccaaggc acaaatcaga tagatcatat tggccatcaa ctaatacaga
     2341 tgctgaaagc gacagttttt cgtctttgag gattgcacgt gctacatctg ttgtgttctt
     2401 tgcaatacca aatatagaaa aaaccttgta ttttcttaga tctttccaat aatttctcac
     2461 attatctgta atttgctgca cctgagtagt gcttaaaaaa tctaaaacag gtttaccgtt
     2521 atatcttgca tgagaaaaaa ttggaaccat tgtatctccg tgttctccaa gtacaagcgc
     2581 atttgtgatt ttgtcaacat ctgtattcaa ttcctttgca agaatatacc taaatctact
     2641 agagtcaagg tttcctgcaa taccaattac cttttctttt gaatggggca cctctttttg
     2701 aaaaacatag gtaataacat caagaggatt tgttacaatt agaatttttg actctggaga
     2761 atgttgtttg atttttttgg caatatcctt gattaggaca acgttttgtt ctaaaaaatc
     2821 catcctacta gttcccgagt tacctgcact ggcagaaatt ataacaactt tagagttctt
     2881 tattttagaa tagtcatcgg ttccaacaac agataccggt gaatccacag acacagaatt
     2941 tgatatatca agtgctgtac ctagcgcctt ttccttgtta cggttaacca aaacaatatc
     3001 atcaagcgat gttgcagcaa taagaaatgc ta
//
LOCUS       DASWKH010000119         4851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1409081, whole genome shotgun sequence.
ACCESSION   DASWKH010000119 DASWKH010000000
VERSION     DASWKH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409081"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..828
                     /gene="hisF"
                     /locus_tag="VGA92_08950"
     CDS             25..828
                     /gene="hisF"
                     /locus_tag="VGA92_08950"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9846569.1"
                     /translation="MSLTKRVIPCLDVANGRVVKGLHFESIKDAGDPVLLAQKYSNEG
                     ADELVFLDITASQEQRETIRSLVSKVAQVIDIPFTVGGGVKTLQHARDILLCGADKVA
                     INTGAVKNPQIITELMELFGRQCIVVAIDAKRNYNVSNGKNIFSEDSKKFWFEVFVYG
                     GKKETGLDAIDWAKKVQELGAGEILLTSIDRDGTKDGYDLILTKAIVDNVSIPVIASG
                     GCGKPNHMLEVFKQTNVDAALAASIFHYETHSVDRVKEYLKVNDVDVRL"
     gene            851..1189
                     /gene="hisI"
                     /locus_tag="VGA92_08955"
     CDS             851..1189
                     /gene="hisI"
                     /locus_tag="VGA92_08955"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9846570.1"
                     /translation="MEKNINDLDFAKGDGLVPVIVQDVNTKEVLTLAYANKESILLTQ
                     KTGNSWFWSRSRNKLWMKGEESGNTQKVREILVDCDSDAVIYLVEPAGPACHTGERVC
                     FHNKLDQQTL"
     gene            complement(1174..1647)
                     /locus_tag="VGA92_08960"
     CDS             complement(1174..1647)
                     /locus_tag="VGA92_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846571.1"
                     /translation="MKAKIIGISIASVVVTALVLAIIFVGPIDVAKPQEKEDPFEGWN
                     RSGPFAINKLEYKLGENIFVVGEGLGPNDVGTMVFVMPNQTTKYISIPFDGADKTDFN
                     QYFKPSISKARKICSVDDLVGVWTVVLSGTNYKPISFEFLNETLRGDEALFQRVC"
     gene            1811..3025
                     /gene="thrC"
                     /locus_tag="VGA92_08965"
     CDS             1811..3025
                     /gene="thrC"
                     /locus_tag="VGA92_08965"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9846572.1"
                     /translation="MTKVSLQCRECKKEYDSTFRYICDECFGPLDVKYEFPPISKNTF
                     VGREHTYWRYFELLPIQNKANIVSIDAGMTPLVRAENLGKKLGLNNLYIKNDSVNPTF
                     SFKDRPAGIAVSKAKEFGLSAVGCASTGNLASATAAHAAKGGFPCYVFAPSDIEHAKI
                     TQALAYGSKFIAVDGTYDDANRIAAQIGDSKGIGIVNINMRSYYVEGSKTLAYEVAEQ
                     LGWQVPDHLVVPVGSGAMLNAICKGFEELQNISLLKDVSGMHIHAAQPHGCAPIVDAF
                     KNNSSEVIPVENPDTIAKSLAIGDPGDGRYVLKRLKQYHSYAEESNNREILDAILLLA
                     QTEGIFTEPAGGVSVAVLKKMVEDGKIDKNDTTICYVTGNGLKATESLMEVLQKPEVM
                     QADLAKISAVVK"
     gene            3027..4361
                     /locus_tag="VGA92_08970"
     CDS             3027..4361
                     /locus_tag="VGA92_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX9846573.1"
                     /translation="MPNITFTIPSVLNKGGGEKKLQITAGSLLESFNKISENMGDDFK
                     RRVLNDDGTPRSLINIYINGKNARFSGGMETVLKDGDEIYILPAVAGGSELSSKDVDR
                     YSRQIMLEEIGYQGQLKLRAAKVCVVGVGGLGNPITTRLVAMGIGKLRIIDRDVIELS
                     NLHRQTMFDESDVGQVKVEVAAKKLKKLNSDVEIESLPISINDYTALDAIEGCDVVID
                     ALDSVNARYALNKACVEKNIPFVTGAAVGVSGQAFTILPHQSACYSCMFPALDEDSMP
                     TCSIEGVHPSILSVIGGIEVAEAVKIILGKKPGLSDKILHVDLENLDFVMTRTFKAEE
                     CPVCGNGKVESTPKEELIIEELCGRNRGKRTFSITPTQKFEIDSEKITTLAKDLKFRV
                     ENQGELGLSMRTDDFSVSFMKRGSAVIVGPKDENDAVLLYKKLLSKKEVMIN"
     gene            4557..>4851
                     /locus_tag="VGA92_08975"
     CDS             4557..>4851
                     /locus_tag="VGA92_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846574.1"
                     /translation="MNNEIGRKLTSLTLMTIMLTWGYSAFAPSVMPEASAANANLFVS
                     AENSQFQNYFSGPMVIEVVVIDSSISDTNEAKGEPDVTANGNQLRMVQATDGNW"
BASE COUNT         1654 a          906 c         1051 g         1240 t
ORIGIN      
        1 aattgaggag gcaaaaaagc tagcttgagt ctaacaaaaa gagtaattcc ctgtcttgat
       61 gtagcaaatg gcagagtagt aaagggattg cactttgagt caatcaaaga tgcaggtgat
      121 cctgtccttt tagcacaaaa atacagcaat gaaggtgcgg acgaactggt ctttttggat
      181 ataactgcat cacaagaaca aagagaaaca attagatcac tagtatcaaa ggttgcccaa
      241 gtaattgaca ttccatttac tgtgggaggt ggagtgaaaa ccctgcagca tgcgcgagac
      301 attttgcttt gtggggcaga caaggtggca atcaatactg gcgcagtaaa gaacccgcaa
      361 atcattacag agctcatgga attatttgga agacaatgca ttgttgtagc aattgatgcg
      421 aaacgaaact acaatgtttc aaatgggaag aacattttct cagaggattc caaaaagttt
      481 tggtttgagg tgttcgtata tggtggaaaa aaagaaactg gtcttgatgc catagactgg
      541 gcaaaaaaag tacaagagct tggggcaggt gagattcttc taacaagcat tgacagggat
      601 ggaactaaag acggctatga cctaattcta actaaagcaa ttgtagataa tgtgagcatt
      661 ccagtaattg cgtcaggtgg atgtggcaag ccaaaccaca tgctagaggt ttttaagcaa
      721 actaatgtcg atgcagcctt ggcagcatcc atatttcatt atgaaaccca ttccgtggat
      781 agggtaaaag aatatctcaa agtaaacgac gttgacgtaa gattataagg aaaaatctaa
      841 ttctaaagac atggaaaaga atatcaatga tttagatttt gcaaagggag acggactagt
      901 tccagtaata gttcaagatg tcaacacaaa agaagttcta acacttgcat atgcaaacaa
      961 ggaatcaatt ttgctaacac aaaaaactgg caactcgtgg ttttggagca gatcaagaaa
     1021 caaattatgg atgaaaggcg aagagtctgg caacacacaa aaagtcagag aaatcctagt
     1081 tgactgcgac tctgatgccg tgatatatct agtagagccg gcaggccctg cctgtcatac
     1141 cggcgagcga gtctgctttc ataacaaact ggatcagcaa actctttgaa aaagagcttc
     1201 gtctccgcgc agtgtttcat ttaggaattc aaatgatatc ggtttgtaat tagtaccgct
     1261 aagtacaacc gtccacaccc ctacaagatc atcaacacta caaatctttc ttgcctttga
     1321 aatagatggt ttgaaatact gattaaagtc ggtcttatca gcgccgtcaa atggaattga
     1381 tatgtatttt gtggtctgat ttggcatgac aaaaaccatg gtgcctacat catttggacc
     1441 aaggccctca cctacaacaa aaatattttc tccaagcttg tactcaagtt tgtttatggc
     1501 aaaagggcca gatcgattcc agccctcaaa tggatcctct ttttcttgag gttttgcaac
     1561 atcgattggc ccaacaaaaa tgattgctag tacaagcgca gtaactacca ccgaagcaat
     1621 ggaaattcca attatttttg ccttcatcgt ttttgattac atcgcaaaaa agataaaaaa
     1681 cgttgtggat gagtcatatt tgcgcgttat tagacctaat taactattat taaattttta
     1741 gcctaaaaaa ttaagtatta ctaaacttga tattaggaca ttttaactat ggtttagtaa
     1801 acgatttttc atgacaaagg tatcattgca gtgcagagaa tgcaaaaaag aatacgattc
     1861 tacttttagg tatatttgtg atgagtgttt tgggccacta gatgtcaagt acgagtttcc
     1921 accaatttca aaaaacacct ttgtaggccg tgagcacacc tactggcgat actttgagtt
     1981 attgccaatc cagaacaagg caaacattgt aagcattgac gctggaatga cgccgttagt
     2041 cagagcagaa aatcttggaa aaaagctagg gctgaataat ctttacataa aaaacgactc
     2101 tgtaaacccc acattttcat tcaaagacag gccggcaggc attgcggtat caaaggcaaa
     2161 agagtttggc ctgtctgcag ttggatgcgc atctactggt aatctggcgt cggctactgc
     2221 ggcacatgct gcaaaggggg gttttccatg ctatgtgttt gccccaagtg atattgagca
     2281 tgcaaaaata actcaggcac ttgcatacgg ctcaaaattc atcgcagtgg atggaacata
     2341 tgatgatgca aacaggattg cagcacaaat tggcgacagc aagggaattg ggattgtcaa
     2401 tattaacatg agatcatact atgttgaagg atcaaaaaca ctggcatacg aggtcgcaga
     2461 acagcttggc tggcaagtgc ctgaccatct tgtcgttcca gtaggaagcg gcgcaatgct
     2521 aaacgcaata tgcaagggat tcgaagaatt gcaaaacatt tccctgctaa aagatgtgtc
     2581 tggaatgcat attcacgctg cacaaccaca cggctgtgca ccaatcgttg atgcgttcaa
     2641 aaataattca agtgaagtca tcccggttga aaaccccgac accatagcaa aaagccttgc
     2701 aataggtgat cctggagatg gccgttacgt tctcaaaaga ctaaagcagt atcacagtta
     2761 tgccgaagaa tcaaacaatc gggaaatcct tgatgcaata cttcttttgg cccaaacaga
     2821 gggaatattt acagaacctg caggtggtgt ctctgttgcg gttctcaaaa aaatggtaga
     2881 ggatggcaaa attgacaaaa acgacactac aatatgttat gtgacaggaa acggactcaa
     2941 ggcaacagag tcactaatgg aagtattgca aaagccagaa gtaatgcagg cagatttggc
     3001 aaaaatctct gcagtggtga agtaatttgc caaacatcac atttacaatt ccatcagtgt
     3061 taaacaaagg tggaggagaa aaaaaactcc aaataactgc aggctcgctt ttagaatctt
     3121 ttaacaaaat ttcagaaaac atgggagatg atttcaagcg ccgagttcta aacgatgatg
     3181 ggactccgcg ttctcttatc aacatctaca tcaacggaaa gaatgctcga ttctctggtg
     3241 gcatggaaac agtactaaag gatggtgatg agatatacat cctacctgca gtggcaggcg
     3301 gctcagaact ctcaagcaaa gatgttgaca gatactctag acaaatcatg ctagaggaga
     3361 ttggttacca aggacagcta aagctaagag cagccaaagt ctgcgttgtg ggagttggag
     3421 ggctaggcaa tccaattaca acaaggcttg ttgcaatggg aattggaaaa ctgcgaatca
     3481 tagacaggga tgtaattgaa ttatcaaacc tacacaggca aacaatgttt gacgaatctg
     3541 atgtcggcca agtcaaagtg gaagttgctg caaaaaaact aaaaaaacta aactcagatg
     3601 tagaaatcga atccctacca atctcaatta atgattatac tgcacttgat gccatagagg
     3661 gatgtgatgt tgtaatagat gcgcttgata gtgtcaatgc aagatatgct ttgaacaagg
     3721 catgtgtcga aaaaaacatt ccatttgtta ctggtgccgc agtaggagta tcgggacaag
     3781 cctttaccat tttgccgcat cagagtgcat gctattcatg catgtttcca gcactagatg
     3841 aagactcgat gcctacctgc agtattgagg gtgtacaccc gtcaatcctc tcagtaatag
     3901 gtggtattga ggttgctgaa gcagtcaaaa taatacttgg caaaaaacca ggcctatcag
     3961 acaaaatact acacgttgat ttggaaaacc tggactttgt gatgactaga acattcaaag
     4021 ctgaagaatg tcctgtatgt ggaaacggca aagttgaatc aacaccaaaa gaagagctaa
     4081 tcatcgagga gctttgtgga agaaacaggg gaaaaagaac attctcaatt acaccaacac
     4141 aaaagttcga aatagattct gagaaaatca ctactcttgc aaaagaccta aagtttcggg
     4201 tggaaaatca gggagaactg ggactttcta tgagaactga tgatttttct gtcagcttta
     4261 tgaaaagggg ttcggcagtc attgttggcc caaaagacga aaatgatgct gttttgctat
     4321 acaaaaaact cttaagcaaa aaagaagtca tgatcaatta aaaatcagac tgacatgtat
     4381 caaaaatcat gaaaatgacc ccgtaatcac cgtatgaaat gtaaatattg caaaatccga
     4441 tcaaaaaaat ggatcaggcg caaaccagcc ttgaaattta ttcaaaaaat catcccattg
     4501 tggataaatt gtccattaat cttatatact gattttcgaa cagaccaact aataacatga
     4561 acaacgagat aggacgtaaa ttaactagtc ttactttaat gacaattatg ctgacttggg
     4621 gatattcggc attcgcacca agtgtgatgc cagaagcctc agcagcaaat gcaaacttgt
     4681 ttgtatcggc tgaaaactct caattccaaa actacttttc aggaccaatg gtcattgaag
     4741 tagttgtgat tgatagttca atcagtgata ctaacgaagc aaaaggtgaa cccgatgtga
     4801 ctgccaacgg taatcaatta agaatggttc aagcaactga tggtaactgg t
//
LOCUS       DASWKH010000120         7690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_671163, whole genome shotgun sequence.
ACCESSION   DASWKH010000120 DASWKH010000000
VERSION     DASWKH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671163"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3012)
                     /locus_tag="VGA92_08980"
     CDS             complement(<1..3012)
                     /locus_tag="VGA92_08980"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX9846575.1"
                     /translation="MRIKIATLAVFFIFTLTLYVPAQAATNYPFVSSWGGMGLTKTGA
                     FTFPQYVAVDEAGTVYVTDLGNSRVQKFDNDGTYLHAWGSKGTGSSQFSSISGIAARN
                     NYVYVVDHELNLVKKFDGTGNFIKSWGSDGVESERLKLPNGIAVSKDNYVYVADTANA
                     RIQKYDSDGKFIKTIGSSGKEQGRFLFPLGVAVDGDGNIYVSDSGNNRVQKFNSEGVF
                     LKSSNTSSAGFKITPDGLAIDSSGNIIVADSANNRILVMNKDGVVQTTFGSTGTANNQ
                     FKIPKGAALDSDGDLFIVDSSNHRIQKFGTADTPEPTQETPTEQITVKPVPNDFTKPT
                     VLPPNDLYIEATGGLTPVSVGKAIAEDESGIKSLTHNAPAEFPLGITTIIWTAIDGAG
                     NVGMATQTVTVNDSTPPRISNLSDIVLEAKGSQNQVKLGNPTVTDLVGVLSITSDAPE
                     TYSLGETIVKWTAIDVAKNVATYAQKVTVTDATPPRIRAPADVSMEAISLNQNQVNLG
                     EPSISDNTEVSSVTNDAPQFFPLGETEIVWTGIDVAGNVGNAIQKITVVDTATPVITQ
                     PQEIIIEAISETSNSVTLVAPNVSDVQEVQITNDAPKFFPIGQTSITWTAIDLSGNNS
                     TATQIISVVDTTAPTLIQPQDITQEATGVAGNLVTLGEPTLEDVTGISSISNNAPLEF
                     PFGSTIVTWTATDNYGNSASADQTITIIDTTPPKITAPKNVQVEATGISENLVMLGEP
                     KVSDLVEIESISNDAPDVFALGDTAVTWAAIDTSENVMTATQIISVVDTTPPEIIIPQ
                     NLVVEATDSDGTSVSIGQATANDVIGVDSITSDSPDIFNLGTTAITWAATDTSGNVVT
                     AIQNVTVVDTTAPTIIPPSDITLEAVSASENTVSLIIPETTDSVSEVIVSNDAPYTFP
                     LGETIVTWTATDEAGNPATATQTVTIEDTTAPAIIVPEDITIEATSKSDNAITLVVPE
                     ASDNVGVSSLTNDAPAAFPVGETIVTWTATDD"
     gene            complement(3090..3866)
                     /locus_tag="VGA92_08985"
     CDS             complement(3090..3866)
                     /locus_tag="VGA92_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9846576.1"
                     /translation="MMLYDTYTIFWREMKRYRKSRSGILIRLIQPAIWIIVIGHTFAG
                     TQPLIEQAGFDGTYLEFMAPGVIILTAIFTSIFGGVNTLWDRRYGFMNKALTAPISRS
                     SIALGKMLAISLISALQSALIIGIAIIIGVSFPNLLVIIPIMAIVILFSLGFSGISVI
                     VAATAKSQETFWGVINFLGMPLFMLSPALFPLELLPDWLATAARLNPVTYTVLLVRGL
                     MTGVTEGISTALSLGIIGAFVVTMIGLASYVFTREVNKPF"
     gene            complement(3863..4867)
                     /locus_tag="VGA92_08990"
     CDS             complement(3863..4867)
                     /locus_tag="VGA92_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9846577.1"
                     /translation="MYAIETRSLTKVYSSDVVAVDDVSIQVNEGEIFGFLGPNGAGKS
                     TIMMILTTLLRPTKGQAFVSGFDIVSQQKKVREQIGYVQQDITIDEYLTGRENLLLQG
                     RLNHIPKNQLNSRIDELLDLVELVDKQDEAAITYSGGMRKRLEIAGGLLHRPKVLFLD
                     EPTVGLDIQTRHKIWEYIKKIHTEYQMTIFLTTHYMEEADRLCDRIGIIDYGKIQAID
                     TPQTLKNALGNGVIIVSIDEHASKPQLVSSIREIEFVRDVTESDGKITIFSSKGPEVA
                     PMIFSLSSKFGITIKSISITQPTLDDVFLSYTGRDLRDSDGTTYDRRKEYRKKERMSP
                     "
     gene            complement(5103..5555)
                     /locus_tag="VGA92_08995"
     CDS             complement(5103..5555)
                     /locus_tag="VGA92_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846578.1"
                     /translation="MATQLEGMTENLCTADDFTGKKVVDREGIGYGKVKHIHINTNTL
                     AVSGVTIHQGFHKDYYLTTEYIDRFTEKSLLLSRPPIRTDVPVVDIDGHKLGKVKRLV
                     KDNETSELQSIEVSDGVLHTKIITKSEIWGVGEKVILKMTKEQFKSSH"
     gene            5643..5960
                     /locus_tag="VGA92_09000"
     CDS             5643..5960
                     /locus_tag="VGA92_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX9846579.1"
                     /translation="MQGKVFKAALITKGRRTGREHSVWLRAVLYNGKVYFSRRNQNSD
                     WLKNAIASPTVKVVFDDQTHVGVASLVTDESLAKKISELKYPSEARAQEARVALQVEL
                     NLP"
     gene            complement(6003..6647)
                     /locus_tag="VGA92_09005"
     CDS             complement(6003..6647)
                     /locus_tag="VGA92_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846580.1"
                     /translation="MAMPMDYDGLRSDEGSERASSVDDYKVTCIRFIEDISKDYLVWV
                     DYYKLPPDEDKKRRDRIASVIQRTNEWIAKIAQTIKGVDVVMNDGIQKMAESTAGQPF
                     QIGVFVNKQSGYTSSKPGVIDVNVKAVGRNGRKTKIIEHYKGQQFRIESTAKAFIDET
                     LLPQGEFDMMDVHLYIDATAAQQRDLISFTISVAEMENDVEMDKRGVSTIIHIV"
     gene            6726..7229
                     /locus_tag="VGA92_09010"
     CDS             6726..7229
                     /locus_tag="VGA92_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846581.1"
                     /translation="MSFSAITAELQLQLKSNLPQIRLLLKKNPAIGYTKITEIGSSVG
                     QKYKIQLLVNFPQREKINEFELYGRRDLSIIVDRQKKNFPIQRSIIKDVAKKMLGDVQ
                     VDDAYMYEGKEGVRVSFEGGRIDILPHSLHIWCLFDDKVTSYCDWLLSEVYLLGKEPS
                     SLTSSKE"
     gene            complement(7181..>7690)
                     /locus_tag="VGA92_09015"
     CDS             complement(7181..>7690)
                     /locus_tag="VGA92_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846582.1"
                     /translation="QYLVKYQDMEFLAELRFLDQLDGEQNIQVIIDDKKTNQQISNTL
                     RIGEAYVFIDVPEDIKPYIHALDLTVFSVRDTLGGSKYLVVGAEWGTTYIGKFTPKLK
                     LTEYGDTQFEFGTLKTYTISYKINDVENKFLIADNIPLPVRAEFYTIEGDLDYSFELV
                     KLEGSLPSK"
BASE COUNT         1920 a         1644 c         1692 g         2434 t
ORIGIN      
        1 atcatctgtg gcagtccatg taactattgt ctctccgact gggaatgcag caggtgcgtc
       61 gttagtaagg cttgatactc ctacattgtc agaggcctct ggaactacta gtgttatggc
      121 attgtctgat tttgatgtgg cttctattgt gatatcttct ggcacaatga ttgcaggtgc
      181 agtggtatct tctatggtaa ctgtttgtgt agcagtagcg gggttgcctg cctcgtctgt
      241 agcagtccat gttactatgg tctctcctag tgggaatgta tacggtgcat cgtttgatac
      301 tattacctcg cttacagagt ctgttgtctc tggaatgatc aacgatacgg tgttctctga
      361 ggcagatacc gcttccaatg ttatgtcgga tggcggaatt atagttggtg cagtagtgtc
      421 aactactgtg acgttttgga ttgcggttac gacatttcca gaagtgtctg tagcagccca
      481 ggtaattgcg gttgttccta aattgaaaat atctggcgaa tcacttgtga ttgaatctac
      541 gccgattaca tcattagcag ttgcttggcc aatagaaacg ctagtgccat cagagtctgt
      601 tgcttccact accaagtttt gtggtattat tatttctggg ggagttgtgt caaccacaga
      661 tattatttgt gttgcagtca tgacgttttc cgaagtatca atagcagccc aggtaactgc
      721 tgtgtcacca agcgcaaata catctggtgc gtcgtttgat attgattcaa tttctacaag
      781 atcagaaacc tttggctccc caagcattac aagattttcc gaaattcctg ttgcctcaac
      841 ttgcacgttc tttggtgcag taatcttggg cggagtagta tcaattattg tgatggtttg
      901 gtctgcagaa gcgctgtttc cataattgtc tgtggcagtc caagttacta ttgtagagcc
      961 aaacgggaat tccaagggag cgttgtttga tattgatgag attcccgtaa cgtcttctag
     1021 tgttggctcg ccaagtgtaa ctagatttcc tgcaactccc gttgcctcct gtgtgatgtc
     1081 ttgcggctgg attagggttg gtgcagtagt gtcaactact gatataatct gagttgcagt
     1141 agagttgttt ccactcaagt cgatggcagt ccaagttatg ctggtttggc caatcgggaa
     1201 aaatttcggg gcatcgtttg taatttgtac ttcttgcaca tcagatacgt ttggtgcgac
     1261 tagtgttact gagtttgatg tttcagaaat tgcttcaata ataatttctt gcggttgggt
     1321 gatgactggg gtcgcagtat caactacggt tatcttttgg attgcatttc caacgtttcc
     1381 tgccacatca attccagtcc atacaatttc ggtttctcca agtgggaaaa attgaggggc
     1441 atcgtttgta actgaggaaa cttcggtatt atcagaaatg gacggctcgc caaggtttac
     1501 ctggttctga tttaggctta ttgcctccat tgagacatct gctggggcgc gtatccttgg
     1561 tggggtcgca tcagttacgg tgactttttg ggcgtacgtt gccacgtttt tggcaacatc
     1621 aatggcagtc cactttacta tggtctctcc aagcgaatat gtctctggag catcactagt
     1681 tatcgacaac acaccaacca gatcagtgac ggtcgggttt ccaagtttga cttggttttg
     1741 cgatcctttt gcttctagta caatatctga cagatttgaa attctaggtg gagtcgaatc
     1801 gtttactgtc acggtttggg ttgccatgcc cacatttcca gcaccatcga ttgcagtcca
     1861 aattatcgtc gtaattccaa gcgggaattc tgcaggtgcg ttgtgtgtaa gcgactttat
     1921 tccgctttcg tcttcggcaa ttgcctttcc aactgagact ggagttagtc cacctgttgc
     1981 ctcgatgtaa agatcatttg gtggaagtac ggttggttta gtgaaatcat ttggcactgg
     2041 ctttactgta atttgctcag ttggagtttc ctgtgttggt tctggtgtgt ctgctgtgcc
     2101 aaacttttga attctgtgat tgcttgaatc tacgataaac aagtctccat cagaatctag
     2161 tgcggcgccc tttggtattt tgaattggtt gttggcagtg ccagtggagc caaacgttgt
     2221 ttgtacaacc ccgtccttgt tcattactag aattctgttg tttgcagaat cggctacaat
     2281 tatgttgcca gatgaatcta tcgcaagacc gtcaggtgtg atcttaaatc cggcagatga
     2341 agtgttggag ctttttagaa aaactccttc agagttgaat ttttgcactc tgttgtttcc
     2401 agagtcgcta acatagatat tgccatcgcc atcaactgcc actccaagtg gaaataaaaa
     2461 tcgtccctgt tcttttccac tagagccaat tgtcttgata aattttccat ccgaatcata
     2521 tttttggatt ctggcgtttg cagtgtctgc aacataaaca tagttgtctt ttgatactgc
     2581 tattccgttt ggaagcttga gtctttctga ctctacacca tcagaacccc atgacttgat
     2641 aaaatttcca gtgccatcga atttctttac taaatttagc tcgtgatcta caacatagac
     2701 atagttgttg cgtgctgcaa ttccagatat agaagaaaat tgagaagatc ccgttccttt
     2761 tgatccccat gcatggagat atgtcccatc attgtcaaat ttctggactc gagagttccc
     2821 aaggtctgtt acatatactg tgcctgcctc gtcaactgcc acgtattgtg ggaatgtaaa
     2881 ggcaccagtt tttgtaagtc ccatgccgcc ccaactgcta acaaaaggat agttggttgc
     2941 tgcttgtgca ggcacataca gtgttagggt gaaaataaaa aatactgcta gcgtagctat
     3001 tttgatccgc atttaggcat aagtttcgag gttagcaaaa gatctcaaaa gtaagaattt
     3061 cttgcagttt tagtcaaatt ttttctgaat taaaacggct tgttgacttc gcgcgtaaag
     3121 acatagctgg caagaccaat catcgtgacc acaaaggccc caattatgcc aaggcttagt
     3181 gcagttgaga tgccttcggt aacgcccgtc atcaggccgc gcaccaagag cactgtgtat
     3241 gtgactgggt ttagtcttgc tgctgttgca agccagtctg gcaaaagctc aagtgggaaa
     3301 agcgcggggc tgagcatgaa taatggcatt cctaaaaagt tgattactcc ccaaaacgtc
     3361 tcctgcgact tggcagttgc cgcgacaatt acagagatgc cggaaaatcc aagcgagaac
     3421 aaaattacaa ttgccattat cggaataatc acaagcaggt ttggaaagct cactccaatt
     3481 atgattgcaa ttccaattat cagcgcagat tggagtgcgg atatcaaaga gattgcaagc
     3541 atcttaccaa gtgcaataga tgatctggag attggtgcgg taagtgcctt gttcataaat
     3601 ccgtaccttc tatcccacag ggtgttgacc ccgccaaaaa tgcttgtaaa aattgcagta
     3661 agaattatga ctcctggtgc catgaactcc agataagttc cgtcaaatcc agcctgctca
     3721 attagtggct gggttcctgc aaacgtgtgg ccaatcacga taatccagat tgcgggctgg
     3781 attagcctta ttagaatacc gcttcgggat ttgcggtatc gcttcatctc acgccaaaaa
     3841 attgtgtatg tgtcgtatag catcatggtg acatcctctc cttttttcgg tattcctttc
     3901 ttctgtcata tgtcgtcccg tcgctgtcac gcagatcacg gcccgtatat gacaggaaca
     3961 cgtcatcgag tgtgggctgg gtgattgaaa ttgattttat tgttatgcca aactttgagg
     4021 aaagagaaaa aatcatcggg gctacctctg gtccctttga tgagaatatg gttatcttgc
     4081 cgtcactttc cgttacgtcc cttacaaatt ctatttccct aattgaggag actagctgtg
     4141 gttttgatgc gtgctcatcg attgaaacta tgattacgcc gtttccaagc gcgtttttga
     4201 gcgtctgtgg ggtatctatt gcctggattt taccatagtc gattatccct attctgtcgc
     4261 acagcctgtc ggcttcttcc atgtagtgag tagtcaaaaa gattgtcatc tggtattcgg
     4321 tgtggatttt tttgatgtac tcccatatct tgtgtcttgt ttggatatca aggcctactg
     4381 tcggctcatc caaaaacaga acctttggtc tgtgcaataa tccccctgca atttcaagtc
     4441 gttttctcat tccgccagaa taagttattg ctgcttcgtc ttgtttgtcg acaagctcca
     4501 ctaggtcaag cagctcatca attctagagt tcagttgatt ttttggaatg tggtttagcc
     4561 ggccctgcaa aagaaggttt tccctcccag taaggtattc gtcaatcgtt atgtcttgtt
     4621 ggacgtagcc tatttgctct cgaacctttt tttgttgtga tacaatatca aaccccgaaa
     4681 caaacgcctg gcccttagtt ggcctcagaa gagtagtaag tatcatcata atggtgctct
     4741 ttcccgcacc gtttgggccc agaaagccaa aaatctcacc ctcgtttacc tgaattgaga
     4801 cgtcatctac tgccacgaca tcagaactat atacttttgt cagcgatctg gtctctattg
     4861 catacattac gatcaaccaa ttggactggc tataaaatgg tgtcttaact ttgttaatgg
     4921 gtaaaaaaat tttgcaaaaa aaatttaagg cagaaaaaat ttttcatttc tatgaagggc
     4981 ctatgccaca tctgctataa ctcaaatgtt gaagtagtct taaatgacgg caccccgatg
     5041 tgccgcaatt gtgcatcaaa gaccaaataa cttgtttttt tagataaaaa attaaaaaga
     5101 aattagtgtg agcttttgaa ctgttctttt gtcatcttta gaattacctt ttctccaacg
     5161 ccccagattt ctgactttgt gattatcttt gtgtgtagta ctccgtctga gacttcaatg
     5221 gattggagtt cgcttgtttc gttatctttg actagacgct ttaccttgcc tagcttgtgt
     5281 ccgtcgatgt caactactgg tacatcagtt cgaattggcg gcctgctgag tagaagcgat
     5341 ttttctgtaa acctgtcaat gtattcggtt gtcagatagt agtccttgtg gaatccttgg
     5401 tgaattgtca ctccagagac tgcaagtgtg ttggtattga tgtggatgtg ttttaccttt
     5461 ccatatccga ttccctctct gtctactacc ttttttcctg taaaatcgtc ggctgtgcac
     5521 agattttccg tcattccttc taattgtgtt gccatggcct gtttttcaag attggtttat
     5581 ggaatacaaa aattacaatt ttcaatcaat gaggttaaat tacttgactt tttggaattt
     5641 gcgtgcaagg caaggtcttc aaggcagctc tgataacaaa gggcagaagg actgggaggg
     5701 agcactctgt gtggttgcgc gctgtcttgt ataatggcaa agtctacttt tcgcgaagaa
     5761 accaaaactc ggactggcta aaaaatgcca ttgcaagccc cacagtcaaa gtggtctttg
     5821 atgatcagac ccatgtcggg gtggcatcac ttgttacaga cgagtctctt gccaaaaaga
     5881 tctcggagct caagtatcct agtgaggcgc gggcacaaga ggcaagagtg gcactacaag
     5941 ttgaattgaa tttgccctaa ttcataaaac ctgattgata acatatacca agttctaaaa
     6001 ctttacacta tgtgtattat ggtggagaca ccacgcttgt ccatctccac atcgttttcc
     6061 atctctgcta ccgaaatagt aaatgatatc aaatcgcgct gctgtgctgc tgtagcatcg
     6121 atgtaaaggt gtacgtccat catatcaaat tccccttgcg gaagtaaagt ctcgtcgata
     6181 aaggccttgg cagtagactc tattcgaaac tgctggccct tgtaatgctc aattatcttt
     6241 gtctttcttc catttcgtcc tactgccttt acattgacgt cgatgactcc cggctttgag
     6301 ctcgtatagc cagattgctt gttgacaaag acaccaatct ggaatggctg gccggcagtt
     6361 gattcggcca tcttttgtat gccgtcattc attacaacat caactccctt gatggtctgg
     6421 gcaatttttg caatccactc gtttgttctt tgtatgactg atgcaatcct gtcccgcctt
     6481 tttttgtcct catctggtgg gagtttgtaa taatcgaccc ataccagata gtcctttgat
     6541 atgtcctcga tgaatcgaat acatgtgacc ttgtagtcat ccacactgct tgcccgctca
     6601 gagccctcat cgcttcgaag cccgtcatag tccattggca ttgccatatc atttggttct
     6661 cactggacgt aaaaaaacca tcccgcgcaa tcaatgaata tatttcaagc atactacaaa
     6721 tcatcatgag tttttctgca attactgctg aattgcagct acagctcaaa tcgaatttgc
     6781 cgcagataag actgctccta aagaaaaatc ctgcaattgg atacaccaaa atcaccgaga
     6841 ttggctcatc tgttgggcaa aagtacaaaa tccagctact agtcaacttt ccccaaagag
     6901 aaaagatcaa cgagttcgag ttgtacggaa ggcgcgacct ttccatcata gtcgacaggc
     6961 aaaaaaagaa ctttccaatc cagcgctcaa tcataaagga tgtggcaaaa aaaatgctcg
     7021 gagatgtaca ggtagatgat gcatatatgt acgagggaaa agaaggggtg cgcgtctcat
     7081 ttgagggtgg cagaattgac attttgccgc attcgctgca catctggtgt ctttttgatg
     7141 ataaggtgac tagttactgc gactggcttt tgtccgaggt ctatttgctt ggcaaagagc
     7201 cctcaagctt gactagctca aaggaataat ccaagtcccc ctcaattgta tagaattctg
     7261 ctctgactgg aagcgggata ttatctgcta tcagaaattt attttcaacg tcgtttatct
     7321 tgtaggatat ggtgtatgtc tttagtgtcc caaattcaaa ttgcgtgtcc ccgtattctg
     7381 tgagcttgag ttttggtgta aatttgccaa tgtaggttgt gccccattct gccccaacaa
     7441 ctaggtattt tgatccaccc agcgtgtccc gcactgaaaa aacagtaaga tccaatgcgt
     7501 gaatgtatgg tttgatgtct tctggcacat caatgaagac atacgcctcg ccaatcctta
     7561 gcgtgtttga tatctgttgg tttgtttttt tgtcgtcaat tattacttgg atgttttgtt
     7621 cgccatcaag ctgatccaaa aacctaagct ctgctaaaaa ctccatgtcc tgatatttta
     7681 ccaaatattg
//
LOCUS       DASWKH010000121         7519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2176631, whole genome shotgun sequence.
ACCESSION   DASWKH010000121 DASWKH010000000
VERSION     DASWKH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176631"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGA92_09020"
     CDS             complement(<1..534)
                     /locus_tag="VGA92_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846583.1"
                     /translation="MKTHFLLMVVTAVLITGFYSVSAHKTVIVEQYEIEVGWRDEPPL
                     VSQQNAIVFAITVDEGGGVQSGVTNAFRDLQATVKSGSITKPLDILSDVRAGHYYSKI
                     IPTKIGSLAIELKGTINGVQVNEEVTIEDVESIDILAFPPTGSGGSNQDVLALKNAMS
                     SLQKDVTEIKSKMGNIAD"
     gene            638..3379
                     /locus_tag="VGA92_09025"
     CDS             638..3379
                     /locus_tag="VGA92_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX9846584.1"
                     /translation="MKKFLILLSLIFVLGIPLAYAHPVILNSSPAQSEAAPVDTTQII
                     INYSEAIEIDFSVIKVLDSNGNQIDNKDTRYFDGESSLVVTTPPLEDGIYTVTSKVLS
                     KVDGHLVPEAFVFGVGNVSVPPPKQKDLGESIYFPEAGARFPGLVGQVIVLGSVISSL
                     VMWKAAQRKNLIKENLVELQKFYHSKFSSITGIALFLVFASNILMLVVQTIRLETSAA
                     NVLQTSFGSVWILRMAITVILLAVWFLMENKTSLSSKKQGLILGLSLVLIGTTTIIGH
                     GTASEQLSAVIIDYIHNLIASVWIGGVIFFGFILLPAFAKLDGDKKELITLLMIPKFS
                     SIIIIALGILIITGPTLLWVLEDDLVALSQSYYGFLIIAKIAVASAMVALGGYNQFKI
                     QRPAEAALKSDNVVVHKKLKRSLKTEAVLGIILLGVVALLTNSSLPTTQTQQATAQIS
                     YGLQTAEFSENIRFDVDIEPFRSGANTISISAFDSNGNPLDDLSNIKVKISNPQKNIV
                     PIEIPLTRSENDPKYEGDVTFGFSGRWNIEVEAQRTQHANEGTSFSVFVKPHISELKT
                     DITEIDLPDSAAPLYPAYDGDDTIWISDASNPRIWKFSISDKQFASYEFEGKTTVFLT
                     IGNDDKIWFTDTPESKIGYFDPIREEFTVISLPAKSIPISLETDLDGNIWVALIDQHM
                     LLKYDQDADQFEEYKTPTNPSGPVALTKDANGDIWFAESQSGKIGVIEPQTGNIQEFA
                     PSEPLKEPFALFIDGDGNVWISEHTGLSITKFNPVLQTFESVAVSDPNSLPFALAPDK
                     FDNIWIAQHTADKLGVYDPHKGEFGEVNIPTTSTATQFLTADKDGNIWFVEQRANKLG
                     SVIISESPQISIVQEQPELDLKYSEFVAPFISAGIIATSMFFVKSVRDKRRLDSMIA"
     gene            3439..5085
                     /locus_tag="VGA92_09030"
     CDS             3439..5085
                     /locus_tag="VGA92_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846585.1"
                     /translation="MDNNGSAESKNEGGYKKRLKDASEARSAEQAKTEQSPQADSELQ
                     PGKIFEESTKSISEMTSEMRETAELVSSMREGLQRVNKIANVIYDNPLYNTQEDHRYS
                     EIERLAAQNSGSSQKSLADVKIAAKQSIDNYKVVDLATDHTKDGLSDLKSDTESIRHE
                     LMQFQNNIKQKESELIEFREKLNSARQQVRGFSDQDLTDNVSSSLLTQISKNAPEESQ
                     DLTEFDAATSLNDSTDFGSIQENELLNSQHEIKEGEKTLLELRWKIKKAEAEYEKRKA
                     EKEEFGDLMAQIQELETKRDSLKVEVKKLETQNKEPKQELKELGQKIEWAKKEYEEKL
                     AAKQELDDVRSVLEYLKLDKESLKSDLEELRVRIKNAEKEYQEKKAAKDEFEDVRFNV
                     TTLKAEKETILAELEQLQAKIKKAESEFEEKRAEKEKLGEFKAVLTHMNIEKESLESE
                     LVELKEKIKKTETEYQEKKAAAEELHEVREVITFLKPERDAVKLELTQLRGKIEKLQD
                     SYDEINARKRELQLEYDEQKFRLRKLESEYEEKKNSLHFR"
     gene            complement(5078..5923)
                     /locus_tag="VGA92_09035"
     CDS             complement(5078..5923)
                     /locus_tag="VGA92_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9846586.1"
                     /translation="MSLEILSYSFMQKGLVAGMAIAIICSLMGIFLVLRRYSLFGDAL
                     AHMAFGGISVGMLAGVYPLWTAFAVSVLGALGITKLRKSTKISGDAAIAVLLVSGLGV
                     GVLLISATDGFRVDLFSFLFGSILLISTEDTLLILGISAGVVAALTALRKQLLHMTFD
                     EEQAKVSGLNIDRLNYIFVVLASITVITSMRLVGILLISALIVLPNITSMLFGKGFKK
                     TVIISMSISVFSVVSGIILSYYLDLAPSGTIVMITVAVLIGTLIAKYAGIVGKSKPLE
                     YQKVT"
     gene            complement(5926..6726)
                     /locus_tag="VGA92_09040"
     CDS             complement(5926..6726)
                     /locus_tag="VGA92_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX9846587.1"
                     /translation="MSMNVLEVNDVSIKYNGYLAVDKINFSVKEGDLLGIVGPNGAGK
                     TTLFKAIIGLQNYTGSIKLFGYEGKDYHPLLPLIGYVSQKVNFEQNFPATVQEVVSMG
                     LLPEKKLVKGAILLQNCGCCWSRIYKKLEKDSDKVLQTLRTVGLESLKDRRIGDLSGG
                     ELQRVFIAKALVKDPVLLILDEPVTGVDMEAQSKFYNLLQTINKENKITIVWSSHDLE
                     AISKLANRVACMNRKLFFHGEKGEFFSNKELLKTYAESAMQMHMHDHD"
     gene            complement(6717..>7519)
                     /locus_tag="VGA92_09045"
     CDS             complement(6717..>7519)
                     /locus_tag="VGA92_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX9846588.1"
                     /translation="VGGEKAEVSSFIPIGIEPHDWEPSSGDILKLKGSDVFVYNGAGF
                     EPFVEQLIDSGEYNDVVFVESAKGIDLILPKHDEEKHDTETEDEHDSEYDPHVWLDPT
                     LVKHQVTTIKNAMIAADPQNAQYYETNANTYNEKLDSLDSKIREELSNCKKDTFMPFH
                     DAFSYFANRYGLKIFALSGVAPESETTAAELKEFIDFVKEHQIKIIFSEELIDPRLAE
                     VLADEAGAQVMILSPLEGLTNEDLAAGKTYISKMEENLENLKVALECQ"
BASE COUNT         2371 a         1514 c         1514 g         2120 t
ORIGIN      
        1 atctgcaatg tttcccattt tggatttaat ttccgtaacg tctttttgaa gtgaggacat
       61 ggcgttcttt agtgccaaga cgtcttggtt tgatcctcct gagccagttg gaggaaatgc
      121 aagaatgtct atgctttcca catcctcaat ggtaacctct tcatttacct gaactccgtt
      181 tatggttccc ttcaactcta ttgcaagaga tccaattttg gttggaatta tttttgaata
      241 gtaatgaccc gccctaacgt ctgaaagaat gtctagtggt tttgtgatac ttcctgactt
      301 gactgtggcc tgaaggtctc tgaaggcatt tgttactccg gattgtactc cgcctccttc
      361 atctaccgta attgcaaata ctatggcgtt ttgctggctt accaagggtg gttcatcacg
      421 ccagcctact tctatttcat attgctcgac aataacggtc ttgtgggcag ataccgagta
      481 aaatcctgtt atcaaaactg ccgtaactac catcaatagg aaatgagtct tcatgatgta
      541 caatattctt actcaagtta tttactttgt gcaaaaactg gtagaaccgt caaactagta
      601 aattgtttaa attagcaaaa tgaccaatca aatacgattg aaaaaatttc taatcctttt
      661 atcattgatt tttgttttag gaattccttt agcatatgct catcccgtaa ttcttaattc
      721 aagtcccgca caatcagaag cagccccggt tgacaccaca cagattataa tcaattattc
      781 agaagccata gagattgatt tttccgtcat caaagttctt gacagtaatg gaaatcaaat
      841 agacaacaaa gatactcgat attttgacgg tgagtcgtca cttgtcgtaa ctactccacc
      901 gttagaggat ggaatctata cggtgacgag taaagttctc tcaaaggtag atggacacct
      961 agtccctgag gcctttgtgt ttggcgtcgg taatgtcagt gtcccaccac caaaacaaaa
     1021 agatttgggc gaatcaatct attttccaga agccggcgct aggtttccag gtcttgtagg
     1081 acaggtaatc gtgcttggtt ctgtaatttc atctcttgta atgtggaagg cagctcagag
     1141 aaaaaatctc atcaaggaaa atcttgtaga gctgcaaaaa ttctatcatt ccaaattttc
     1201 ctccattact ggcattgcgt tgtttttggt atttgcatca aacattttga tgctagttgt
     1261 ccagaccatc cgtcttgaga catcagctgc caacgtcttg cagacatcgt ttggctccgt
     1321 gtggatactc agaatggcaa taactgttat tttgcttgcg gtgtggtttt tgatggaaaa
     1381 caagacaagc ctttcctcaa aaaaacaggg gttgattctt ggactatcac ttgtattaat
     1441 tggcacaaca acaataattg gacacggcac ggcaagcgag cagctttctg cagtaataat
     1501 tgactatatc cacaatctga ttgcgtcagt ttggattggt ggtgtgatct tttttgggtt
     1561 catcttgttg cctgcttttg ccaaattaga cggcgacaaa aaagagctaa ttacgctatt
     1621 aatgatcccc aagttttctt caataattat cattgcgctt ggaattctga taataactgg
     1681 gcccacattg ttgtgggtcc tagaagatga tcttgtggca ctaagccagt cctactatgg
     1741 ttttctaata attgcaaaaa ttgctgttgc gtcagcaatg gtggcacttg gcggctacaa
     1801 tcagtttaag atacaaagac ctgcagaggc ggcgctgaaa tcagataatg ttgttgttca
     1861 taaaaagctg aaaagatcac taaagactga agcagtgctt gggattattt tgcttggagt
     1921 cgttgcactg ctcacaaatt cttctttgcc aacaacgcaa acacagcaag caactgcaca
     1981 gatatcatac ggattacaaa ctgcagagtt ttcagaaaat atcagatttg atgtagatat
     2041 tgaaccgttt agaagtggtg caaatacaat atctatttct gcctttgatt ctaacggaaa
     2101 ccctcttgat gatctaagca atatcaaggt aaagatctct aatccgcaaa aaaacatagt
     2161 acctattgag atcccactaa ctagatcaga aaatgatcca aaatatgagg gcgatgtgac
     2221 gtttggtttc tcaggaagat ggaatattga agtcgaggca cagagaactc agcacgcaaa
     2281 cgagggaacg agcttttctg tctttgtaaa accgcacatc tctgagctaa aaacagacat
     2341 caccgagatt gacttgccag acagcgcagc accgctttat cctgcgtatg acggtgatga
     2401 caccatatgg attagtgatg cctcaaatcc gcggatatgg aagttttcaa tttctgataa
     2461 gcagtttgca tcatatgagt ttgaaggaaa aacgacagtg tttctaacaa tcggcaatga
     2521 tgacaagatt tggtttacgg atacgcctga aagtaaaatt ggctattttg atcccatcag
     2581 agaggaattt acagtcattt ctcttcctgc aaaatcaatt ccaatctcgc ttgaaacaga
     2641 ccttgatggt aacatctggg ttgcgttgat agatcagcac atgttgctaa aatatgatca
     2701 agacgcagat cagtttgaag agtacaaaac tccgacaaac ccatcaggtc cagttgctct
     2761 gacaaaagat gcaaatggcg acatttggtt tgcagaatca caaagcggga aaattggtgt
     2821 aattgagcca caaactggaa acattcagga gtttgcacca tctgaaccgc taaaagagcc
     2881 gtttgcactt tttattgacg gtgatggtaa cgtttggatt tcagaacata ctggtttgag
     2941 cataaccaag tttaatccag tgttgcagac atttgagtca gttgcagtgt cggatccaaa
     3001 ttcacttccc tttgcccttg cgccggataa atttgataac atctggattg cgcaacacac
     3061 agcagacaag cttggtgtgt atgaccccca caaaggtgag tttggcgagg tgaatattcc
     3121 gacaacatcc acagccacgc aatttttgac tgcggacaaa gatggaaata tctggtttgt
     3181 tgaacagcgt gcaaacaagc ttggaagtgt catcatatct gagagccccc aaattagcat
     3241 cgtacaggaa caaccagaac ttgatctgaa atattctgaa ttcgtagcac cgtttatctc
     3301 tgctggaata attgcaacct cgatgttttt tgtaaaaagc gttcgcgata agcgaaggct
     3361 tgattccatg atagcgtaaa agcgtttgtt cttttcgaac gacatgacgg tattatctga
     3421 cttaatacaa tttttcatat ggacaataat ggttcggctg aatcaaaaaa tgaaggtggc
     3481 tataaaaaac gactaaagga tgcgtctgag gcaagatctg cagaacaggc aaaaacagaa
     3541 caatccccac aagccgactc tgaactgcag cctggcaaaa tattcgaaga aagcacaaaa
     3601 tccatttcag aaatgactag tgagatgagg gaaactgccg agcttgtttc atccatgaga
     3661 gaaggccttc aacgagtaaa caaaattgct aatgtcatat acgataatcc tttgtataac
     3721 acgcaggaag accacagata cagtgagata gaaagactgg ctgctcaaaa ttctgggtca
     3781 agtcagaaat cattagctga tgttaaaatc gctgctaaac agtcaattga caactacaaa
     3841 gttgttgatc ttgctaccga ccatacaaaa gatggcctat cagatttaaa atcagatact
     3901 gaatcaatac gacacgaatt gatgcagttc caaaataata ttaaacaaaa ggaatcagag
     3961 ttgatcgaat tcagagaaaa gctaaattct gcacgacaac aagtaagggg attttcagat
     4021 caagatctca cagataatgt cagttcaagc ttgctaactc aaatatctaa gaatgcacct
     4081 gaagaatcgc aagatcttac ggaatttgat gccgccacgt cactaaacga ttcaacggat
     4141 tttggttcaa tacaagaaaa tgaattacta aattcccaac acgagatcaa agaaggagaa
     4201 aagacactcc ttgagttacg atggaagata aaaaaggcag aggcagaata cgagaaaaga
     4261 aaagcagaaa aagaagagtt tggggatttg atggcgcaaa ttcaagaatt agaaacaaaa
     4321 cgagatagtt tgaaagtcga ggtcaaaaag ttagaaaccc aaaacaaaga gccaaaacag
     4381 gaactaaaag aattaggaca aaaaatagag tgggctaaaa aagaatatga agaaaaactg
     4441 gctgcaaagc aagaactaga tgacgtaaga tcagtccttg aatacctcaa actagacaaa
     4501 gaatccctca agtcagacct agaagagctt agagtgagga tcaaaaacgc tgagaaggag
     4561 tatcaagaaa aaaaggctgc aaaggacgaa tttgaggatg taaggtttaa cgttaccacg
     4621 ctcaaagcag aaaaggagac tattttggca gaacttgaac aattgcaagc aaagatcaaa
     4681 aaggctgaat ccgaatttga agaaaaacga gccgaaaaag aaaagcttgg agagttcaaa
     4741 gccgttctaa cccatatgaa tattgaaaag gaatcacttg aatctgagct tgtcgaactc
     4801 aaagaaaaga tcaaaaaaac agagacagaa taccaagaaa agaaggctgc tgcagaggaa
     4861 ctacatgagg ttcgtgaagt cattacgttt ttgaagccgg aaagagacgc agtaaaattg
     4921 gagctaaccc agcttagagg gaaaattgaa aagctgcaag acagctatga tgaaataaat
     4981 gcaagaaagc gagaactgca gctagaatat gacgagcaga agtttaggct aagaaagctg
     5041 gaatcagaat acgaggaaaa gaaaaactcg cttcacttta ggtaactttt tgatattcaa
     5101 gtggtttgga ttttcctaca atcccagcat attttgcaat cagagtccca attagaactg
     5161 caactgttat catcacaatt gttccagacg gcgccagatc cagataatat gacaaaatta
     5221 ttccgctaac tacagaaaac accgaaattg acattgagat aattacagtt ttcttaaagc
     5281 ctttcccaaa cagcatgctt gtgatgtttg gcagaacaat cagtgcagaa atgagcaaga
     5341 ttccaacaag tcgcattgag gtaattacgg taatactggc aagaacaacg aaaatgtagt
     5401 ttagtctatc aatatttagg ccgcttactt ttgcttgctc ctcatcaaat gtcatgtgaa
     5461 gcagttgttt tcgtaaggct gtcagtgcag caacaactcc ggcactaatt cccaagatga
     5521 gcaaggtgtc ttctgtgctg atcaaaagaa tgcttccaaa aaggaagcta aacaaatcga
     5581 ctctgaaccc atctgtggcg ctaatcaaaa gcacgcctac accaaggcca gacaccaaaa
     5641 gcactgctat tgcagcatct ccagaaatct tggtgctttt tctaagctta gttattccca
     5701 gcgcacctaa cacagatact gcaaatgcag tccaaagtgg gtacacccca gctagcatcc
     5761 ctacagagat tccaccaaat gccatatgtg caagcgcatc accaaaaaga gaataacgtc
     5821 ttaagaccaa aaatatgccc atcagtgaac aaattattgc aattgccatt cctgcaacca
     5881 atcccttttg catgaagctg tagcttagaa tttcaaggct catggttaat cgtggtcgtg
     5941 catgtgcatc tgcatcgcag actcggcata tgtctttaag agctccttgt ttgagaagaa
     6001 ttcacccttt tctccatgga agaacagttt tctgttcatg catgcaaccc tgtttgctag
     6061 cttggagatt gcttctaggt cgtgagaaga ccacactatt gtgatcttgt tttctttgtt
     6121 tattgtttgc aacaaattgt aaaacttgga ttgtgcttcc atatctactc cagtcactgg
     6181 ctcatcaagt atcaaaagta ctggatcctt gacgagtgct tttgcaataa agacccgttg
     6241 cagctctcca ccagacaggt cacctattcg tctgtctttt agtgactcta atccaacagt
     6301 cctcagtgtc tgaagaacct tgtcgctatc cttttctaat ttcttgtata ttctgctcca
     6361 acaacaaccg caattttgta aaaggatggc acctttgacg agtttctttt ctgggagaag
     6421 acccatagaa acaacttctt gaacagttgc aggaaagttc tgctcaaaat tgactttttg
     6481 agaaacatag ccaatgaggg gaagcaatgg gtggtagtct tttccttcat atccaaacag
     6541 tttgatgctg cctgtgtagt tttgcagccc aattattgcc ttaaacaggg ttgtctttcc
     6601 agcgccgttt ggtccaacga ttccaagcag atcaccttct tttacgctaa aattgatttt
     6661 atctacggct agataaccgt tgtattttat tgaaacatca tttacttcca atacattcat
     6721 tgacattcaa gtgccacctt tagattctcc agattttctt ccattttaga tatgtaggtt
     6781 ttaccagctg cgagatcctc gttggtaagg ccctccaagg gacttaggat cattacttgt
     6841 gcgccagcct catcagcaag aacctccgca agccttggat caattagttc ttcggaaaat
     6901 atgattttaa tttggtgttc ttttacaaaa tctatgaatt cttttagctc cgctgcagtt
     6961 gtttctgact ctggtgcaac tccagaaaga gcaaagatct ttaacccata cctgtttgca
     7021 aaataactaa aggcgtcatg aaaaggcata aaggtatctt ttttgcagtt tgatagttct
     7081 tctcttattt tcgaatcgag actatcaagt ttttcattgt atgtgttggc atttgtttca
     7141 tagtattgtg cgttttgtgg gtctgctgca atcattgcat tctttatcgt cgtgacttgg
     7201 tgttttacaa gagttggatc cagccagaca tgtgggtcat attcagaatc atgttcgtct
     7261 tctgtttcag tatcatgttt ttcttcatca tgtttaggta atatcagatc aattcctttt
     7321 gcgctttcta caaaaaccac atcgttgtat tctcctgaat caattaattg ctctacaaat
     7381 ggttcaaatc ctgcaccgtt atagacgaaa acatcggatc ctttaagctt caatatgtca
     7441 ccgctgctcg gctcccagtc atgcggttct ataccaattg gaataaaact tgaaacttcg
     7501 gctttctcac cgccaacat
//
LOCUS       DASWKH010000122         6245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1805153, whole genome shotgun sequence.
ACCESSION   DASWKH010000122 DASWKH010000000
VERSION     DASWKH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805153"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VGA92_09050"
     CDS             <1..1109
                     /locus_tag="VGA92_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846589.1"
                     /translation="SGNILSVTLPAGVSGVITFTDTDSGSSDQSIEFFGDIIDIDPTG
                     GASCATGCTVSFIFTGTDAADEGLSPSDAVVYHDSNENGSFESGEGLVTTVTGSDPYT
                     ATATASFTSKFAVGGVRANVAAAAQSLSAGGFFKNLSSCESNGFDSGVSLRVYSISYD
                     KCENNQLQVLAYSTCGPIVAEVTTKIGRYTLGISSTQPFLNDKEKKIALGANISPELE
                     SFNILIKDKRDSFTDRIMLNQCNATKTYTHTTGYTSEQQGLLVRNDTKSQITPASIEQ
                     TKPDIPPWIKNNAKWWSSGQIADSEFTQGIEYLIKQDIINVPESTQTDTAQESKQIPN
                     WIKTNSRWWADGMISDSEFIQGLEYLIKVGIIRV"
     gene            1169..1732
                     /locus_tag="VGA92_09055"
     CDS             1169..1732
                     /locus_tag="VGA92_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846590.1"
                     /translation="MEYSLEFFATIGHVMTGIGTMMLSVLLYKTFRHMEASTRATELQ
                     TEYKFRPWVGPIGTVREINDDSDKKRFEITIRNYGEYAATDVTAASLVKSEKITRDDL
                     KSKPNGHVSLGPLLPNMEKRFWFDIDNQTIKKTKETNTKIFVGITIEYPLVVGNSEYG
                     MISEVDPETLSFVHKDMWVQSPPLRSK"
     gene            complement(2077..3279)
                     /locus_tag="VGA92_09060"
     CDS             complement(2077..3279)
                     /locus_tag="VGA92_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9846591.1"
                     /translation="MEIPTPTRLFHAHFLFVDIVGLSDPSMSTKTQVKKIETLNKSIS
                     ETTTYKNTPKDTILVLPTGDGMCLGFLQGPELPLHLAIDLHKKLDEYNKGKIPSETVR
                     IRIGLHSGSCFIVKDIRGYDNVWGPGTIISRRVMDFGDDGHILLSTRLAEDLRELSDE
                     YRKIIKPVHDFTIKHGQTMLVYSAYGPGFGNPQHPTKGAAERSKYGEEILKMQKTALY
                     PNLEVHLTITDPEKMLTRHKRTYEVANISDEPIKYVLHGIATDVEKYSINDLNLQVLD
                     DANREMRISSINVDKPTTKEFTTQFNRPVVKGEKDRKYTLVYEVEEPERYFENAFLID
                     VQKFSLVFEYPKSMRDCQPIIYYINQETDEKTKSEIDLIVEETPDCFITRYNKAENLK
                     GETLRLEW"
     gene            3404..4702
                     /locus_tag="VGA92_09065"
     CDS             3404..4702
                     /locus_tag="VGA92_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9846592.1"
                     /translation="MQKFDFAIIGGGILGTTTSYWLSSLYDARVCVIEKDGIASHASS
                     KNTGVVHSPFYLDPQKRKTIAKAALASHGLWKDFALAKNISWKQCGTIEVALDEKQHK
                     TLEKYDKWGKENGISDNELELLDSTQVSKKEPNVRCYSGIYCSRDASTDYGALTNELS
                     RVSEKQGTKLLLGHHVQDIKSKDDGIELGFDDGSNLECKFLINCAGGHSLDIAKKLGI
                     AQDYSTLHFRGEYWIAQNQCANLVNTNIYSVAKFSNFPFLDPHWVKRANGQTEIGPNA
                     VPVADPETYSGYVGNIPKTLSKLREILSGNSRKLLFNSEFLTLVSKEWKSSLSKTAMV
                     NRVKQFIPAIKPSFFTKRGTAGIRSPVITKKGEFLPEILELETKNSLHIINYNSPGAT
                     GAPAYAAFMVKKLQDKGILNLKTKQNNGIWDFQKIIEQMP"
     gene            4748..5008
                     /locus_tag="VGA92_09070"
     CDS             4748..5008
                     /locus_tag="VGA92_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846593.1"
                     /translation="MLPDRCSIKEKGRNCVNPPEFVISVLGDKDEYMIGVSCENHKNV
                     ISGKLEILQKEGKIPKGKINFVKLKAVGTDCIKASPDDLIQL"
     gene            complement(5016..5987)
                     /locus_tag="VGA92_09075"
     CDS             complement(5016..5987)
                     /locus_tag="VGA92_09075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX9846594.1"
                     /translation="MTSNKPRVLITRRLHDFALKELKKRYDIFVHDGKIPMPKKLLRE
                     KIKKADALVCFPYDIIDRRIIECAPNLKVISTYSVGFDHIDTKAAKKHGIKIGYTPEV
                     LTNATADLTVALLLDLLRRVTEGDRLIRAGNWKIIFGAYDYVGVELEGKTLGIFGMGR
                     IGQAVAKRAHSFGMKIIYHNRKKLPHTLEKKLHAKYVSFGDLIRKSDVLSIHVPYTKD
                     THKIINHTALQKMKKTSFLINTARGKIINESDLAKALRDGLIAGAALDVFEYEPIGKR
                     HELTKMPNIVLAPHIGSSTAETRRKMAEITMQNLILGLDGKKMIYSV"
BASE COUNT         1923 a         1227 c         1254 g         1841 t
ORIGIN      
        1 catcaggaaa tatcctctca gtaacccttc cagcaggggt atcgggcgtc atcacattta
       61 cagacacaga ttcagggtct tcagatcaaa gtatagaatt ctttggagac ataatagata
      121 ttgatccaac cggtggggca agctgcgcca caggatgtac agtatcgttt atctttacgg
      181 gtacagatgc tgcagatgag ggtcttagcc cctctgatgc agtagtgtac catgattcaa
      241 atgaaaatgg cagcttcgag tcaggcgaag gccttgtcac cacagtcaca ggctcagacc
      301 catacactgc aactgcaacc gcgtcattta catcaaaatt cgcagtaggt ggagtcaggg
      361 ccaatgtagc tgcagcagca caatcgcttt cagccggcgg atttttcaaa aacctttcat
      421 catgtgagtc aaatgggttc gattctggag tgtcactgcg agtgtattca ataagctatg
      481 acaagtgtga aaacaaccag ttgcaggtgt tggcatactc tacgtgcggg ccaattgtag
      541 cagaagtaac aaccaaaatt ggaagataca cacttggaat atcgtctact cagccattcc
      601 taaatgataa agaaaaaaag atagcacttg gagcaaacat ctcgccagag ctggaatcat
      661 ttaacatcct gataaaggac aaaagagatt cctttaccga cagaataatg ctaaaccagt
      721 gcaatgcaac aaagacatac acacatacta ctggatacac ctcagaacaa caaggattac
      781 ttgtcagaaa tgacacaaaa tcacaaatca ctcctgccag cattgagcaa accaaaccag
      841 atattccacc gtggataaaa aataatgcaa aatggtggtc ttcagggcag atagccgatt
      901 ctgaatttac tcaaggaata gaatacctca tcaaacaaga catcattaac gtaccagaat
      961 ctacacaaac ggacactgcc caagaatcaa aacaaatccc aaattggatc aagacaaact
     1021 cgcggtggtg ggcagacggc atgatatctg attcagagtt tatccaggga ttggaatatt
     1081 tgataaaggt tggaataata cgtgtataaa atccaaatcc ttattttctt aattttttgc
     1141 agtgatctca gggggcaggt aaaataacat ggaatacagc ttggaatttt ttgcaacaat
     1201 tgggcatgtg atgactggaa taggaacaat gatgctgtcg gttcttctct acaagacatt
     1261 cagacacatg gaagcttcta cgcgtgcaac cgaacttcaa accgaataca agttcagacc
     1321 atgggttggg ccaattggta cagttaggga gataaatgat gattctgata aaaaacgatt
     1381 tgagatcacg ataagaaatt atggtgagta tgcggcaact gatgttacag ctgcaagtct
     1441 tgtaaaatcg gaaaaaataa caagggatga tctaaagtca aaaccaaacg gccatgtcag
     1501 tcttggacca cttttgccaa acatggaaaa aagattctgg tttgacatag ataatcaaac
     1561 aatcaaaaaa acaaaagaaa ccaacacaaa gatttttgtt ggaatcacta tagaataccc
     1621 actagtcgtt ggaaacagcg aatatggaat gataagcgag gttgatccgg agacacttag
     1681 ttttgttcat aaggatatgt gggtgcagag ccctcctctg agatccaaat aatatttcat
     1741 tctggaccca aaatgaaagc catttttcca tggtgcttat catgtttgtg gcacaattaa
     1801 ttatcatgac ctctctttca aaattctgat aggtgatacg aaatgaacgt gatttgttcc
     1861 ctaatgaaca ttctaaatgg tcaaatcaac agacttgctt ttggatttca cgcggtgatc
     1921 ttttgtatca agttgggaaa atgattctat tttttgattc ttattatgag attgtttccc
     1981 atcccaaaat ctactcatac cataacaatt acgagttggt gcaataagga acagtgtgtt
     2041 tagttgaacg cacaaaattt tgccggtttt ataagattac cattcaagcc tcagggtttc
     2101 acctttgagg ttttcagcct tgttatatct agtaataaaa caatctgggg tttcttccac
     2161 gatcagatct atctctgact ttgttttttc gtccgtttct tgattaatat agtaaattat
     2221 aggttggcaa tctctcattg attttgggta ttcaaaaaca agtgagaact tttgaacatc
     2281 tattaaaaac gcgttttcaa agtatctttc tggctcttct acttcgtaaa ctagggtgta
     2341 ttttctgtct ttttcgccct ttacgacagg cctgttgaac tgtgtggtga attcttttgt
     2401 ggttggcttg tccacattaa tgcttgatat tctcatttcc ctgtttgcat catcaagtac
     2461 ttgcaaattc agatcattga tggaatattt ttcaacatcc gttgcgattc catgcagaac
     2521 atattttatc ggttcatctg aaatgttggc tacctcatag gttcttttgt gtcttgtgag
     2581 cattttttca ggatctgtta tggtaaggtg tacctcaaga tttggatata gtgcggtctt
     2641 ttgcattttg agaatctctt caccgtattt gcttctctct gcagcgcctt ttgttggatg
     2701 ttgtggatta ccaaatcctg gaccatacgc tgaatacacg agcattgtct gaccgtgctt
     2761 aattgtgaaa tcatgtactg gtttgattat ttttctatat tcatctgata attcacgcaa
     2821 atcttcagct agtctagtgc taagaagaat atgaccatcg tctccaaaat ccatcactct
     2881 tcttgagatt attgtaccgg gaccccaaac gttgtcataa cctcttatgt ctttgacgat
     2941 aaaacagctg ccactgtgca gtcctattcg tatccttact gtctcagatg gaatctttcc
     3001 cttgttgtat tcgtctagtt ttttatgaag atctattgct aaatggagcg gtagttctgg
     3061 tccctgcaaa aagccaaggc acataccatc tccagtaggt aggacaagaa tggtatcttt
     3121 tggagtgttc ttgtatgtgg tagtttcgct tatgcttttg tttagggtct caattttttt
     3181 tacttgggtt ttagtcgaca tggatggatc tgataagccc acaatatcca cgaaaaggaa
     3241 gtgggcatga aataatctag ttggtgtagg aatttccatt atgtctaatt tagtaatctg
     3301 ctacataaag ttgagttcaa acccatacgt cccgcgcctt ttatcaaaaa ttaataatcc
     3361 ttttacgcat agaaattaat ggttaggcct ccgttgtttt gtattgcaga aatttgattt
     3421 tgccataatt ggtggaggta ttcttggtac cacaacatcg tactggcttt cttctttgta
     3481 tgatgcaaga gtgtgcgtta ttgaaaaaga tggtattgcc tcgcacgcaa gcagtaaaaa
     3541 caccggcgtg gttcactcac cgttttatct ggatcctcaa aaaagaaaaa caattgccaa
     3601 ggctgccttg gcttcccatg gcttgtggaa ggattttgca ttagctaaaa acatttcttg
     3661 gaaacaatgc ggcacaatcg aagtcgcctt ggatgaaaaa caacacaaaa cactagaaaa
     3721 atacgacaag tggggaaaag agaatgggat ttctgataac gaactagaat tgttggattc
     3781 aacacaggtt tcaaaaaaag aaccaaacgt taggtgttat tctggaattt attgcagtcg
     3841 tgatgcgtca acagattatg gtgctctgac aaatgagctt agcagagtat ctgaaaaaca
     3901 aggaacaaaa ttactgctag gacatcacgt acaagatatc aaaagcaaag acgacggtat
     3961 agaattagga tttgatgacg gctcaaatct agaatgcaaa ttcttgataa actgcgcagg
     4021 aggacactcg ttagatattg caaaaaagct tggcattgcc caagattatt cgacacttca
     4081 ttttaggggg gaatactgga ttgcgcaaaa ccaatgcgcc aatttggtga acaccaacat
     4141 ctattctgtt gcaaaatttt ccaactttcc atttttggat ccgcactggg ttaaaagagc
     4201 gaatggccaa acagaaattg ggccaaatgc agttccagta gccgatccag aaacatattc
     4261 tggatacgta ggaaatatac caaaaacact atcgaagctc agagaaatac tcagtggaaa
     4321 ttcgcgaaaa ctgctcttta attcagaatt tttgacattg gtctcaaaag aatggaaaag
     4381 ctcactatcc aagactgcca tggtaaatcg cgtaaagcag ttcatcccgg caatcaagcc
     4441 atcgtttttt acaaaacgtg gaacggcggg aatacgaagc ccagtcataa caaaaaaggg
     4501 cgaattcctc ccagaaattt tggagctaga gaccaaaaac tcgcttcata tcataaatta
     4561 taattcgcca ggtgccacag gtgctcctgc atacgcagca tttatggtaa aaaaactgca
     4621 agacaaggga attctaaatt tgaaaaccaa gcaaaacaat ggaatctggg attttcaaaa
     4681 aattatcgag caaatgccct gaatacacta atcaaaatta atattgattt gaagaaacca
     4741 agaatcattg ttacctgaca gatgttcaat taaggaaaaa ggccgtaact gtgtaaaccc
     4801 accggagttt gtcatctctg ttttgggtga caaagatgaa tacatgatag gcgttagctg
     4861 cgaaaatcac aaaaacgtca tttctggaaa actcgaaatt ctccaaaaag aaggaaaaat
     4921 tccaaaaggc aagataaact ttgtaaaatt aaaggcagtt gggactgact gcatcaaggc
     4981 aagccctgat gatctaatcc agctttgagg ctggctcaaa cagaataaat catctttttt
     5041 ccatccagtc ctaaaatcaa attctgcatt gtgatttctg ccatttttct tcgtgtctct
     5101 gcagtcgagc tgccgatatg tggtgctaaa acaatgtttg gcatttttgt cagctcatgg
     5161 cgttttccaa ttggctcgta ttcaaaaaca tctagggcgg caccagcaat caatccatcc
     5221 cttaaggcct ttgccaaatc actttcgttg attatcttgc cgcgtgcagt attgattaaa
     5281 aacgaagtct ttttcatttt ctgtagggct gtatggttta tgattttatg agtgtctttt
     5341 gtatatggca catgaattga aagaacatcg cttttcctaa tcaaatcgcc aaagctgaca
     5401 tatttggcat gaagtttttt ttccaaagtg tgtggcagtt tttttctatt gtgatagatt
     5461 atcttcattc caaacgagtg cgcccttttt gccactgcct gcccaattct tcccatccca
     5521 aatattccaa gcgtttttcc ctcaagctca actcccacat agtcatatgc gccaaaaata
     5581 atcttccaat taccagccct gatcagcctg tctccctcag ttacccggcg caacaagtca
     5641 agcaacaatg caactgtcaa atccgcagtg gcgttagtga gaacttcggg ggtgtatcca
     5701 attttgattc cgtgcttttt tgcagctttg gtatcaatgt ggtcaaatcc aacactgtat
     5761 gtgcttatga ctttgagatt tggcgcacat tcaattattc tcctatctat gatgtcataa
     5821 ggaaagcaga caagggcatc tgcttttttg attttctctc tcagcagttt ttttggcata
     5881 ggaatttttc catcatgtac aaagatgtcg tatctttttt tcaattcctt tagggcaaaa
     5941 tcatgcagcc tgcgagtaat gagaactcgg ggtttgttgc tagtcacttt atgcgaaaag
     6001 tcaatcaaat ctttataaga tttgttgtgt gatccaaggt aactttgaag aaaaagaagg
     6061 gtaacgatga agaagaattc gcactgtgcg tcgaatgtgg aaacgcaata gaaaagtgcg
     6121 tttgcgtatg tccatactgc ggcgagcgag acaagtgcga gtgcgccctt tttgaggctg
     6181 ctaccggtgg gtaagaaaat taaacatatt cttaataatc aacaaatcat aggagacctg
     6241 tgact
//
LOCUS       DASWKH010000123         5682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3267470, whole genome shotgun sequence.
ACCESSION   DASWKH010000123 DASWKH010000000
VERSION     DASWKH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267470"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..664)
                     /locus_tag="VGA92_09080"
     CDS             complement(230..664)
                     /locus_tag="VGA92_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9846595.1"
                     /translation="MAGTFVHDIMKKNVISIDSSMTAQDAAKMMDDASIGAIVVLENG
                     IAIGIVTERDLARRIVAKGKSLTTNVKEVMSSPLIVINPDETVWEAAQLMKTRKIHRM
                     PAVKENRLVGIVTTSDIVKLCSIGSDSELRKITEQILLRAKL"
     gene            786..1160
                     /locus_tag="VGA92_09085"
     CDS             786..1160
                     /locus_tag="VGA92_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846596.1"
                     /translation="MVILESLAGFEPKKAKESLEMLLVDRKNEFKDLAKGIGIPTSAP
                     NWEHMILKFCLDFNQCFKEVLTTVEGPNDANEYSHNKIHQCMTLLRQISRGKTSMIEV
                     THLQNLAYTIAEEFKTVYKRLS"
     gene            complement(1163..1441)
                     /locus_tag="VGA92_09090"
     CDS             complement(1163..1441)
                     /locus_tag="VGA92_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846597.1"
                     /translation="MSMHNRATCVFCKSPRSIHADKTQWLRHLSTHREAIIAYIVDHY
                     EKCPLGAYPRLIKDKTEYAGHLRWAHSRRALYEWVHRNLIENQVVVYP"
     gene            complement(1448..1924)
                     /locus_tag="VGA92_09095"
     CDS             complement(1448..1924)
                     /locus_tag="VGA92_09095"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9846598.1"
                     /translation="MVLVPDKVKKLIQKEQILVVGSSDKTGISNVSPRTTYYLGTDGS
                     IYWLELFKHKTYRNFQKNPWCSIAVFDKKRLVGYQLKGKATIVTDKKSKNEIVIKIVD
                     KLTRLHRQRILKQNKKPNLVKFTPNIVYSLNPNELTDSPLVLDATDESLNTANMQW"
     gene            complement(1983..2408)
                     /locus_tag="VGA92_09100"
     CDS             complement(1983..2408)
                     /locus_tag="VGA92_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846599.1"
                     /translation="MVTNYSRILVCYDGSKYSDGALRQACDLAKRYDSALTLIHVIQK
                     TKKSDLLAGNEYTQILRKYAKSALVKAQKIAKEQGFEPKIVTKEGNAANEIVKYSKTD
                     KTDLIVVGSKGLGAVLQIFLGSVSSKIADRSLCSVLIVK"
     gene            2683..3108
                     /locus_tag="VGA92_09105"
     CDS             2683..3108
                     /locus_tag="VGA92_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846600.1"
                     /translation="MKNKIKRILVPMDGSKTSFKALDNAIEIARVCHATILGVHVISF
                     LPTEFMPAVVPYKIYQKKEAGIFLEKSKTLAAKKGILFKYAIIYGNPVEQIISLARAK
                     KFDLIVIGARGKGRLAEVFLGSVSNAILHKSTIPVMVVK"
     gene            3119..3580
                     /locus_tag="VGA92_09110"
     CDS             3119..3580
                     /locus_tag="VGA92_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846601.1"
                     /translation="MYQRILVPFDGSKFSKNALNEAIKIAQTTNGIIYLCTIISMGNI
                     VPPGSLLGLVRSASTGDLQRRLIRSAKIQAERLEQSQVSYCESKGVKAYYKIIVNGNI
                     AEEILKVAKKKTIDLIVIGSQGLHGIGKIKSLGSVSRKVSELASCPVLIVR"
     gene            complement(3584..4213)
                     /locus_tag="VGA92_09115"
     CDS             complement(3584..4213)
                     /locus_tag="VGA92_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX9846602.1"
                     /translation="MIHDRLHAGRLLAEKLTPLVKNKDCLVLAIPRGGVVVGAEIARI
                     LGVSLDVIISKKITPPDYPEYAIGAVTYDGVLYYGHDWERYSTDPRFEEEINKKKAEV
                     KRQIESYRGTTSYDFDNKTVILVDDGIATGATVCAILEWLSQKKVKDIILAIPVVPFV
                     THEILKRFGVQIVALEYPLEFSAVGQFYRKFEQVPDQIVLNLLQKYKSK"
     gene            complement(4261..5127)
                     /locus_tag="VGA92_09120"
     CDS             complement(4261..5127)
                     /locus_tag="VGA92_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9846603.1"
                     /translation="MLLKNPGLRRSIIKKAITVTQNTLVLEAIEILQKFRIGRLVVIG
                     KNYAPVGIITQKDILRSLYPLGNKPLGSLQVGDMMSTNLITVRKSDSVYRCAKLMKDN
                     NISSLVVLRDDNSLEGIITKTDLVFNFLIQETEPLKVSKVMTAKVIIVAPDDQLFFVE
                     SVLLNNKISRVPVVKNQKLLGIITYKDFIPAKIPNRLGSFTDPSELRDIWYSPHPSQF
                     NINQLDYVLTFRAEDIMTKNPVTIESSDDVSMAALVMYRYNISGIPVIKQSKLAGIIT
                     KSDIVFALAKEA"
     gene            complement(5137..5565)
                     /locus_tag="VGA92_09125"
     CDS             complement(5137..5565)
                     /locus_tag="VGA92_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9846604.1"
                     /translation="MTNSKFKSILVALDGSKGALKALDEAIYLARQNQATITGVFVIP
                     LFSVNVAKPASKMGKLFVDGGKKILAEAKTHCAKNGVLFYEKILCGNEGFKIVSFAKN
                     KKADLIVVGSRGRSNVRELFLGSVAHYVLHKSQIPVLVVK"
BASE COUNT         1681 a         1075 c         1072 g         1854 t
ORIGIN      
        1 atgaatcata ttagttcacg aaagaaatct gaggatttgt tttctgtcta taattgctat
       61 tgaacctaat attgccaagc caatgattag acttgcttca ggagaaaata cccgtactga
      121 taaatcccac aacccacgcc aaaacaaaat gattccaact atgccagcag tgaaaacaaa
      181 tgcttgtttt cgtttgaagg ttgtttcagg tatgtttcat tgtgttctat tacagctttg
      241 ctcgcaaaag aatttgttca gttattttac gtaattcaga atcagaacca atactgcaaa
      301 gcttgacaat atctgatgtt gtcacgatgc ccacaagcct gttttccttt actgcgggca
      361 tcctatggat tttacgtgtt ttcataagct gggcagcttc ccagacagtc tcgtctggat
      421 taattactat gaggggtgaa gacattactt ccttgacatt agttgtaagt gattttcctt
      481 ttgcaactat tcttctagct aaatcacgtt cagttacaat acctatggca ataccatttt
      541 ccaatactac tattgcacca atggacgcat catccatcat ctttgcggca tcttgagccg
      601 tcattgatga gtcaattgag atgacatttt ttttcataat atcatgaaca aatgttcctg
      661 ccatatcaga aaagacacgt tgctgtttaa aagatcaaaa cacgattatc aaacctagta
      721 ttatgggaaa atcatgcgtg agtttcttga aatatgttaa atattattta cccagtttcc
      781 aagatatggt tatacttgag agcctagctg gctttgaacc aaagaaggca aaagaatccc
      841 ttgaaatgtt actagtggat cgaaaaaatg aattcaaaga tctagcaaag ggaataggca
      901 ttccaactag tgctccaaat tgggagcata tgatcttgaa gttctgtctt gattttaatc
      961 agtgttttaa agaagtgtta actactgttg aaggcccaaa tgatgccaac gagtattcac
     1021 acaacaaaat ccatcaatgt atgactttac ttcgacagat atccagaggg aaaaccagta
     1081 tgattgaagt tacccatctg cagaatctcg cttatactat tgcagaagaa ttcaagacag
     1141 tttacaagcg attaagttag atttacggat aaaccacaac ttggttttcg atcaaattac
     1201 gatgcaccca ttcataaagc gcccttcttg aatgtgccca tcttaggtga cccgcatact
     1261 cggttttgtc tttgattagc cttggatagg ccccaagtgg acatttttca taatggtcta
     1321 cgatatacgc aataatagct tctcggtgtg tggaaagatg ccttagccac tgagttttgt
     1381 ctgcatgaat ggaacgtgga ctcttacaga atacacatgt tgcccgatta tgcatgctca
     1441 tagcacatca ccattgcatg tttgcagtat taagagactc gtctgttgca tccaaaacta
     1501 gtggtgagtc agtaagctca tttggattca gcgaatatac gatgtttgga gtaaatttta
     1561 caaggtttgg ctttttgttt tgttttagaa ttcgctgtct atgaagtctg gtaagcttgt
     1621 ccacaatttt gataacaatc tcattttttg attttttgtc tgttacaatt gttgcttttc
     1681 cctttagttg atatcctaca agtctttttt tatcaaagac tgcaatgctg caccatggat
     1741 ttttttgaaa gttgcggtat gttttgtgtt tgaaaagctc aagccagtaa attgaaccat
     1801 cagtacccaa atagtatgtt gttcttgggg acacgttact gattcccgtc ttatcagagg
     1861 aaccaactac tagtatctgt tctttttgaa taagcttctt taccttgtct gggactagga
     1921 ccacagttga ttttctagtc gatttgtaat aaaagcaaga tatgattatc agttttggaa
     1981 gctcatttta ctatcaaaac agagcaaagc gagcgatctg ctatttttga cgaaacggac
     2041 cctaggaaaa tctgcaatac tgccccaagc cctttgctac caacaactat gagatctgtc
     2101 ttatcagttt tggaatattt tacaatctcg tttgcagcat tgccttcttt tgttactatt
     2161 tttggctcaa agccctgctc ttttgctatt ttttgtgcct ttacaagcgc ggatttggca
     2221 tattttctaa gaatttgtgt gtattcgttt cctgccaaaa gatcggattt tttcgtcttt
     2281 tgaattacat gaattagggt aagggcagaa tcatacctct tggcaagatc gcatgcctgg
     2341 cgtaaggcac catcggaata ctttgatcca tcatagcaaa caagtattct ggaatagttt
     2401 gtaaccatat cattaaagtg attctatgaa tgcatttaat gattttgcaa atttttaaaa
     2461 cagaaaaaga cgagaatgca aatacgccat accaaggcat gctacgttgg gttaaaaccc
     2521 cgcattcccg tccatctgca caatattact tgtgcaccgt tattatgtat ctgttatagt
     2581 taagggccta acacgattat cagatctgga accataatgt gattattagc cgtgataatc
     2641 taaacaagac gtttaaatta aaataagaaa aactcttcga tgatgaaaaa caaaataaaa
     2701 agaatccttg tcccaatgga tggatcaaag acttctttta aggcattaga taatgcaatt
     2761 gaaatagcaa gggtctgcca cgctacaatt ttaggtgttc atgtaatttc atttcttccg
     2821 acagaattta tgccagctgt tgttccctac aaaatttatc agaaaaaaga agctggcata
     2881 tttttggaaa agtcaaagac tttagctgca aaaaaaggaa ttttgttcaa atatgctata
     2941 atttatggca atcctgttga acagatcata agcttggcaa gagccaaaaa atttgattta
     3001 atagtgattg gcgcaagggg taaagggaga ctagctgagg tatttttggg aagtgtctca
     3061 aatgcaatac tccataaatc caccatccca gtaatggtag taaaatagtg agaaaacgat
     3121 gtaccaacga attcttgttc cttttgacgg ctctaaattc tcaaaaaatg ctctcaatga
     3181 ggcaataaaa atagcacaaa cgacaaacgg cataatctac ttgtgcacaa tcataagcat
     3241 gggaaatatt gttccgcctg gttcactatt aggtttagtg agaagcgcat caactggaga
     3301 tttgcaaaga aggctgataa gatcagcaaa aattcaggca gagcgattag agcagtctca
     3361 agtttcttac tgtgaatcaa agggagtaaa agcctactac aagataattg ttaatggaaa
     3421 tattgccgag gaaattctca aagttgccaa aaagaaaaca attgacctca tagtaattgg
     3481 aagtcaaggc cttcatggaa taggcaagat caagtctttg ggtagtgtaa gcagaaaagt
     3541 ctcagaactt gcgtcttgtc ctgtcttgat agtaagatag cttctacttt gatttgtatt
     3601 tttgaagtag gttcagtaca atttgatcag gaacctgctc aaattttctg tagaattgtc
     3661 caacagcaga aaactctagt gggtattcta atgcgacaat ttggactccg aatcttttga
     3721 gaatctcatg agtcacaaat ggaaccacag gtattgccaa gatgatgtct tttacctttt
     3781 tttgtgaaag ccactccaaa attgcacaga ctgtagcgcc ggtggcaatt ccatcatcaa
     3841 ctaaaattac agtcttgtta tcaaaatcat agcttgtagt cccacgatat gactcaatct
     3901 gtcttttgac ctcagctttc tttttgttta tctcttcttc aaatcgtgga tcggtggaat
     3961 aacgctccca gtcatggcca taatacaaaa ctccgtcata cgttactgcc ccgattgcat
     4021 actctggata atcaggtgga gtgatttttt tagaaattat aacatcaaga gaaaccccaa
     4081 gaatccttgc aatttctgcg cccacaacca cgcctcctct tggtattgcc aaaacaagac
     4141 aatctttgtt ttttacaagc ggggttagtt tttctgcaag aagccttcct gcatgcaatc
     4201 tatcgtgtat cataggccat tattggtgct tcaggtatac taaattgtgg gcagtaaatc
     4261 ttatgcctct tttgcaaggg caaacacgat atcggatttt gtgatgattc ccgctagttt
     4321 ggattgtttg atgaccggta ttccgcttat gttataacga tacatgacaa gtgccgccat
     4381 ggaaacatcg tctgaactct ctattgtaac tgggtttttg gtcataatgt cttctgccct
     4441 aaaggtaagc acatagtcta actgatttat gttgaattga ctgggatgtg gactgtacca
     4501 gatgtctctt agttcgcttg gatctgtgaa tgaacccagt ctgtttggta tttttgctgg
     4561 gataaaatcc ttgtaggtaa taattcctaa aagcttttga ttttttacga caggcactct
     4621 ggaaatcttg ttgttaagaa gtacactttc gacaaaaaat aattggtcat ctggagcaac
     4681 aatgatcact ttagctgtca ttacttttga cactttgagt ggttcagttt cttgaattaa
     4741 gaaattaaaa acaagatcag tctttgttat aattccctca agagaattat cgtcgcgcaa
     4801 aacaacaagt gagctaatgt tgttatcctt catgagcttg gcacatctgt ataccgaatc
     4861 gctttttcgt actgtgatca gatttgttga catcatatcc cctacttgta gcgaaccgag
     4921 tggtttgttc ccaagcggat aaagtgatct tagtatgtcc ttttgtgtta tgataccaac
     4981 gggggcatag tttttgccaa ttacaaccag ccttccaatt ctaaattttt ggaggatttc
     5041 tattgcttca agaacaagcg tgttttgagt aacagtgatg gcctttttta ttatcgatct
     5101 tcgtagtcca gggtttttca aaagcatggt ttttcactat ttcaccacta ggactggaat
     5161 ttgtgatttg tgtaagacgt aatgagcgac actacctaag aaaagttctc gtacgttgct
     5221 tcttccacga gagcccacga ctatcaagtc ggcttttttg ttttttgcaa aagagacaat
     5281 cttgaatccc tcgtttccac acaatatctt ttcataaaat aacacgccat tttttgcgca
     5341 atgtgttttt gcttcagcca agattttttt tccaccatca acgaacagtt ttcccatctt
     5401 tgatgctggt tttgcgacat tgactgaaaa aagcggtatt acaaagacgc ctgtaattgt
     5461 tgcctggttt tgtcttgcaa ggtaaattgc ctcatctagc gcttttaaag ccccttttga
     5521 tccgtctaat gcaacaagta tgctcttgaa ttttgagttt gtcaagttat catgtgtaag
     5581 atttaaacca aaattaaagc agatcatgat tatctgaatt gattttcagg attgataatt
     5641 cacacccatc tcatatagcg gcgctttact ttgatatttg at
//
LOCUS       DASWKH010000124         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_300907, whole genome shotgun sequence.
ACCESSION   DASWKH010000124 DASWKH010000000
VERSION     DASWKH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300907"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGA92_09130"
     CDS             complement(<1..362)
                     /locus_tag="VGA92_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846605.1"
                     /translation="MARKTNFSHTKKNVIRYPKARIHVSCVIRDEEGDIVAIKGMKLG
                     SSSVVRDQVWTAEEIYEKSGQSPNFSQTHSFWAQDKDSNESEVYCKRKNGKICLVAPS
                     IVDEDGRDILKDLPVCDS"
     gene            complement(469..1464)
                     /gene="dph2"
                     /locus_tag="VGA92_09135"
     CDS             complement(469..1464)
                     /gene="dph2"
                     /locus_tag="VGA92_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HEX9846606.1"
                     /translation="MIVIDENTIFKVIQERKPVSVALNGPDGILPKVQETAVKIMSRF
                     GIPAYVIADTTWGSCDINANGAKVLSAEILFNIGHTISMQVFESNVVLIDAFDDVSFD
                     VVAQKSAEILKEKTVSLVTDSQHLHQIESVKKTLEDNGVLVKIGKGRGQLNDGQVFGC
                     EFYPAIELKDQVDANVFMGQSNFHAAGLALATNKPTFVLDPYFNEVREVTEFAQKLQR
                     KATLSIYKAAEAKTFGVIVGLKEGQISKTTALKFKRELEKEGKTVQLFALTDITNERL
                     RNLKGIDAFIQVACPRISTDNQFDKPVLSTPQANALLKILRNESIEGYLQIPHWL"
     gene            1546..2874
                     /locus_tag="VGA92_09140"
     CDS             1546..2874
                     /locus_tag="VGA92_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008300099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HEX9846607.1"
                     /translation="MNKDTILNIGFDDTDSPKGMCTTYLAYKLVDSLKKENVRFLDYP
                     RLIRFNPNIPWKTRGNGAVALKIQTNSPKMIKKKVIRFVRKFSDLKNGANPGVVFFEN
                     DVIPKEFTKFSDMALWRLISRKDAKKLISENRMESFHLGNGQGLVGAIGAIGYRFNDH
                     TFELLSYRKKSHFGKKRKIISSSVQKMQEKTYPQTYNSYDDKKNRILFAPHGPDPVFF
                     GIRGEDPKSVLFASKLIQAHERPVGHLIFKSNQGTGDHLQNELDVSSLLPYLSGTITG
                     TVSRLPQMEKGKHVTFSIIKNGVEVSCAVYRPTGLTKEAMHLIKCDKIRVGGGIRKSS
                     PKHGRTLNVEFFKVLKLQKKTVLVNPLCAKCNKRMKSKGRNQGFECIKCGQKAQNKTV
                     LKVPRQIKTKLYIPQPSAHRHLTRPQQRIGITNRQTSFDEKDPWFHTYQN"
     gene            complement(2881..2988)
                     /locus_tag="VGA92_09145"
     tRNA            complement(join(2881..2916,2934..2953,2970..2988))
                     /locus_tag="VGA92_09145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2935..2937),aa:Met,seq:cat)
     gene            3067..4161
                     /locus_tag="VGA92_09150"
     CDS             3067..4161
                     /locus_tag="VGA92_09150"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016940015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9846608.1"
                     /translation="MNKKIRIAGIIAALAVSAIILSSPSQSPPLPEPITQPENESIQI
                     LATNLQKPWAIAFADGKIFVTEKQGGIRIIESDTLIEEPLAALRVAKVFGGGLLGIAT
                     HPDFANNHFLYVYYTYSEDDNLWNKVLRITEADNKIKDAITIIDKIPASQFYNGGVIK
                     FGADNKLYVATGLSSEFSHESQDVSSLGGKILRLDDDGTIPTDNPFPDSSVFSLGHRD
                     IQGMAWDKNGNLFVTEFGPTKNDEINLIRAGQNHGWPEVECGGNEEFVDSIMCYDPSI
                     EPGGIVFYSGDKLEYTGSLIMATLRGSSLYKLDISENEVFSQKSILSGVGRIRDVAQG
                     PDDYLYVITSNTDGKGFPDKTDDKLIRILK"
     gene            4163..>4909
                     /locus_tag="VGA92_09155"
     CDS             4163..>4909
                     /locus_tag="VGA92_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HEX9846609.1"
                     /translation="MDSSIPRFFEKSRKERLDIVAKFANLTKEERQILENTSGIGFEQ
                     ADKMVENAIGTFSFPLGIATNFLINKKEYLIPMVIEEPSVIAAASKAAKIARIHGGFT
                     MQSNESYSIGQIQVVNVDVKSAAAKVLASSKEILELANSKSKTLSTMGKGAKEVSCKE
                     LSTDVGQMLIVELLIDVGDAMGANVTNTMCEGVAPLIEKITGGKTILRILSNYSTRRL
                     VRGMATFDKEELGGTQIVDNIISAFQFADND"
BASE COUNT         1581 a          964 c         1010 g         1354 t
ORIGIN      
        1 tcggaatcgc aaactggcag gtcttttaga atgtctctgc cgtcctcgtc aacaattgat
       61 ggcgcaacaa gacaaatttt gccgttcttt cttttacagt aaacttcgga ctcgttgctg
      121 tccttgtctt gcgcccaaaa agaatgagtc tgcgaaaagt tggggctttg tccacttttt
      181 tcatagattt cttctgcggt ccaaacttgg tcacgtacaa cagagctgct tccaagcttc
      241 attcctttga ttgctacaat gtcgccttcc tcatctctga ttacgcagct aacgtgaatc
      301 ctagcctttg gatatcgaat aacattcttt tttgtatgtg aaaaattggt tttgcgcgcc
      361 atttatatca aattataata gacaaaaatt ggtttttgaa ttcaaattac ttccttatca
      421 atgatcatct tgaaatcatc aatattaaca tttttgtttt tgatgtgatc aaagccagtg
      481 tggaatctgc aaatagcctt caatgctttc atttcgtaaa attttgagca acgcgtttgc
      541 ttgcggtgtg gacagaaccg gcttgtcaaa ctgattatca gttgaaattc ttgggcacgc
      601 aacctggatg aaggcatcaa ttccttttag gttgcgtaat cgctcatttg taatgtcagt
      661 taaggcaaac agctggacag tctttccttc tttttctaat tcgcgcttga acttgagcgc
      721 agtcgttttt gaaatctggc cctcctttag gccaactatg actccaaatg tttttgcctc
      781 ggctgccttg tagattgaca gtgttgcctt tcgctggagc ttttgcgcaa attctgttac
      841 ctctcttacc tcattaaagt aaggatcaag aacaaaggtc ggcttgtttg ttgccagagc
      901 aagacctgct gcatgaaagt tgctttgtcc catgaaaaca ttggcatcca cttgatcttt
      961 tagctcaatg gcgggataga actcgcaccc aaacacttgg ccgtcgttta gctggcccct
     1021 accttttcct atttttacca gaactccatt gtcttcgagg gtttttttta ccgactcgat
     1081 ttgatgtaga tgctggctgt cagttacaag tgagactgtt ttttctttca aaatttctgc
     1141 agatttttgc gctacaacat caaaagaaac atcatcaaac gcatcaatta gaacaacatt
     1201 gctctcaaag acttgcatgc taattgtatg tccgatattg aatagaattt ctgcggaaag
     1261 gactttggcg ccatttgcat taatgtcgca ggaaccccaa gtcgtatctg ctattacata
     1321 ggctggaatt ccaaacctgc tcattatttt cactgcagtt tcctgcactt ttggcaaaat
     1381 tccgtcaggg ccattaaggg ctacagatac gggcttgcgc tcctgaatta ctttgaagat
     1441 ggtattttca tctattacta tcattgaaca gctaaccaag ctttgatttt aatttaaacc
     1501 caactaatag taaatcacaa atgtctggtt agtttcttga ctgctgtgaa taaagacaca
     1561 atactaaaca ttggatttga tgatactgat tcgccaaagg gaatgtgcac tacatatctt
     1621 gcatacaagc tagttgattc actcaaaaaa gaaaatgtca ggtttttgga ttaccctaga
     1681 ctgattagat tcaatccaaa cattccttgg aagacgcgtg gaaatggcgc tgtagcacta
     1741 aaaatacaaa caaattcgcc taagatgatc aaaaaaaagg taattcggtt tgttagaaaa
     1801 ttctccgacc taaaaaacgg cgcaaatcct ggtgtcgtat tctttgaaaa tgatgtgatt
     1861 ccaaaggaat ttacaaaatt tagcgatatg gcactatgga gactaattag tcgtaaagat
     1921 gcaaaaaaat taatctcaga aaatagaatg gagtcgtttc atctgggcaa tgggcagggt
     1981 ttagttggtg caattggtgc aattggatat agatttaatg atcacacctt tgagttgcta
     2041 agctatagaa aaaaaagtca ttttggcaaa aaaagaaaaa taattagttc cagcgtacaa
     2101 aaaatgcaag aaaaaacata cccgcaaaca tacaacagct atgatgacaa gaaaaatcgc
     2161 attttgtttg cgccacacgg accagacccc gtcttttttg gaatccgagg tgaagaccca
     2221 aaatcggtgt tgtttgcatc aaagctgatc caagcacatg aaaggccagt cggtcatctg
     2281 atcttcaagt ctaatcaggg aacaggtgat catctgcaaa acgagcttga cgtatcatca
     2341 ctactaccat atctttccgg aaccattact ggcacagtat caagactgcc gcaaatggaa
     2401 aagggtaaac atgttacatt ctcgataatc aagaacggtg ttgaagtaag ctgtgcagta
     2461 tacagaccaa ctggattaac aaaggaggca atgcatctga tcaaatgtga taagattcgt
     2521 gttggaggag gaattagaaa gtcatcacca aaacatgggc gtacactaaa cgtagagttt
     2581 ttcaaagttc tcaaattaca aaaaaagaca gtattggtta atccattatg cgctaaatgt
     2641 aacaagagaa tgaaatctaa agggcgaaat cagggctttg agtgcatcaa gtgcgggcaa
     2701 aaggcgcaaa acaaaactgt gttgaaagtt ccaagacaaa ttaaaaccaa attatacatt
     2761 ccacagccat ctgcacaccg acatctgacg cggccgcaac aaagaattgg tattacaaac
     2821 aggcaaacaa gctttgatga aaaagatcct tggttccaca catatcaaaa ttaaagatac
     2881 tagcgaggag tagatttgaa ctactgatct gcgggtttcg catcaattat gattatgagc
     2941 ccgccgggat gacttcgtcc tatgaaagtc tgacttcccc acctcgctga ctaaaaatga
     3001 aactacaggt gatatataga ctctcatttt gtaaataatt aataagaatt cacaattctt
     3061 ttggttgtga acaaaaaaat aagaatagct ggaatcattg ctgcactagc tgtatctgca
     3121 ataattctga gctcgccttc ccaatcaccg ccgctgccag agcctataac acaaccagaa
     3181 aatgaatcaa tccagatact tgcaacaaat cttcaaaaac catgggcaat tgcatttgca
     3241 gatggcaaga tctttgtaac agaaaaacag ggtggcatta gaataatcga gtcagataca
     3301 cttattgaag agcctctggc tgcgcttcga gtcgcaaagg tgttcggagg aggattgtta
     3361 ggaattgcaa cacatccaga ttttgctaac aaccattttc tgtatgtgta ttatacttat
     3421 tcagaagatg acaatctctg gaacaaggtt ctacgtatta cagaagctga caacaaaatc
     3481 aaggatgcca taacaattat tgataaaatt cctgcctcgc aattctataa cggtggcgta
     3541 atcaagtttg gagcagacaa caagctgtat gtcgcaacag gactatcctc cgaattttca
     3601 cacgaatcac aagatgtatc atcacttggt ggaaaaattc tcagactaga tgatgatgga
     3661 accataccaa ctgacaatcc atttccagac tcgtcagtat tctcactagg acacagggac
     3721 atacaaggaa tggcttggga caaaaatgga aacttgtttg taacagaatt tggcccaaca
     3781 aaaaatgacg agataaacct gatcagggct ggccagaacc atgggtggcc agaagtggaa
     3841 tgtggtggga acgaagaatt tgtagattcg ataatgtgtt atgatccaag catagagccg
     3901 gggggcattg tgttttatag tggcgacaag ctagaataca cgggcagctt gatcatggct
     3961 actttgcgtg gttccagtct ttacaaatta gatattagtg aaaatgaagt tttctcacaa
     4021 aaaagcatac taagtggagt cggtcgaatc agggatgtgg cacaagggcc tgatgattat
     4081 ctttatgtta tcacatcaaa cacggatgga aagggatttc ctgacaagac ggatgacaag
     4141 ctgattcgaa ttttgaagtg aattggattc atcaattcca agattttttg aaaaaagcag
     4201 aaaggaaaga ctggatattg tagccaagtt tgcaaacctc acaaaagaag aaaggcaaat
     4261 tcttgaaaac acaagcggca taggctttga gcaggcagac aagatggttg agaacgccat
     4321 aggaacgttc tcatttccac ttgggattgc aacaaatttt ctcattaaca aaaaagagta
     4381 ccttatcccg atggtaattg aagagccatc tgtaatagca gctgcatcca aggcagccaa
     4441 gatagcaaga atccatgggg gatttacaat gcaaagcaac gaatcctaca gcattgggca
     4501 aatccaagtc gtaaatgttg acgtaaaatc ggcagcagca aaggttctgg catcatcaaa
     4561 agagatactg gaactggcaa attcaaagag taaaacactt tccacaatgg gcaagggagc
     4621 aaaagaagtc tcctgcaagg agctttctac tgatgttggc caaatgttga ttgtagagct
     4681 actaatcgat gtcggagatg ccatgggtgc taacgtgaca aatacaatgt gcgaaggagt
     4741 cgctccacta attgaaaaaa taactggtgg aaaaacaatt ctgcgaatac tttcaaacta
     4801 ctcaacaaga aggctagtca gaggtatggc aacctttgat aaagaagagc tagggggaac
     4861 gcaaatagtt gataacatta tttcagcatt ccaatttgcc gacaacgat
//
LOCUS       DASWKH010000125         5751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2535411, whole genome shotgun sequence.
ACCESSION   DASWKH010000125 DASWKH010000000
VERSION     DASWKH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535411"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..892)
                     /locus_tag="VGA92_09160"
     CDS             complement(158..892)
                     /locus_tag="VGA92_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846610.1"
                     /translation="MISITSTMLLLMAFMLTNNQNSQVAAQNSFVDYKESKMPELRYY
                     QNYKELMASPKTAVVYPIFTQNAYEWEGIHDYYAGYCDSCLSNQIRTTYEKTYAASGN
                     GFRILEFLGYQVIDDMDIDKNPQILNQFDKIILLHNEFVTKKEFEALMAHPKVVYLYP
                     DSLSSEIVVDHAKNSMTLVRGPSYPDSSIKNGFDWENDNAQFFTDWDCNSWEFYTVKN
                     GHMLNCYPETFLPNDGYELLKALKNL"
     gene            complement(1013..1699)
                     /locus_tag="VGA92_09165"
     CDS             complement(1013..1699)
                     /locus_tag="VGA92_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9846611.1"
                     /translation="MKLVRKGHRPFHRGVIGVESAIVMIAFVIVAAALAFVVLNMGFS
                     TTQRAKTAIVASLDESSSALEIAGKVTAAGDASPVGYLNVTAVPVKVASGGSSVNLGN
                     TTMAVRYFTGTVSYDNIMTGVLSSGNYENLTLAMDAAETAGYINVNPITSNTAADATA
                     AVVYFAVNRNNNSILDQGEHAIIAIIHKGDERPSALDVIRAEVIVPTGSPLTIERLVP
                     NITTSVVDLG"
     gene            complement(1992..2717)
                     /locus_tag="VGA92_09170"
     CDS             complement(1992..2717)
                     /locus_tag="VGA92_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaellin/type IV pilin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9846612.1"
                     /translation="MNLIRKGHRQSHRGVIGVESAIVMIAFVIVAAALAFVVLNMGFS
                     TTQKAKTTIVAGLGEASSSLEIGGKVTGFGCTSGSSETACGTAPLLNVTTIPLRIASG
                     GESIDLSSSTASVKYLSNTVQYDNIYAGPITDDVYDNATEAFTRATAESGSAFDAFNS
                     AHPITGATPSSTKAIIYWAVRTNTNAILDQGEHAVMAIAYQTADRPASLDKIRAEIVV
                     PTGAALTIERQVPNITNLVVDMG"
     gene            2980..3783
                     /locus_tag="VGA92_09175"
     CDS             2980..3783
                     /locus_tag="VGA92_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9846613.1"
                     /translation="MSVQEELVTALSYFGLEKTDAMVYLGLLQMGSVTVGTMSAKLDI
                     DRGKAYRSLNKLRNLGLITTTFSNPTICKAMDPKEGLTTIMQRREDEIITMQKLSRKI
                     VEQLQQITRPETVTEISSFSIIQGRANIYARVGKLIQESTKTIYVVTTVEDILRMYHT
                     AIPEKIKMCKQNKGEVRVLTWTNSENLLPLINRLNATETRVGKLPSKSRMIVEEGNQL
                     IMSGSIKESMDLNDDNDSIMYTNSSEMINNMFSLCTHLWKKAKPIELLM"
     gene            complement(3813..>5751)
                     /locus_tag="VGA92_09180"
     CDS             complement(3813..>5751)
                     /locus_tag="VGA92_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9846614.1"
                     /translation="DKAIQINPNYYEVHYQKGMILFGNKKYEDAIACFDNALAINPYD
                     SNLIYQKGVILYYNGKYSVAITCFDKVLELDQSNFLAFYHKAQSLAKLDKHDEALIHF
                     ERALIIQSGHMDSIFGKALALENLNRYEESLIILDKIMEVEPNFVEALYHKGFVLSEL
                     GRHANALTFFDKALELDQNHTNAILGKAILLQQQGLHQDALVYFDKALEIDPANLDVL
                     YHKGRLLMELNKHQNALQIFDQILASNYDHVPTLFSKGLIFEEMGYDEEALRCFDQVL
                     SKSENDVDSLFHRGKVLFNMKKFDNAASSFDEVLEVDPKHVESTVYKAKSLFNLGMLK
                     DSILYSDKALALERNNLDAWYTKSLALIKGAKYQEALRCTEQISKIKPQYKETLYYQG
                     LCLSNLDKSEQALEILDRFLQIHPNHISCLIQKGILFQKLGKHQEAVLCFDKILQLDS
                     SHNLALSRKAQSMAILGDYQNALLCYDMMNKTDSDKEILLEEGLLLSILGQHEKSIEC
                     YDQILKDNPNHFVALKNKSLSLTGLGKYAESLDLLTKALYMEPDNLELLSAKAKSLQV
                     LGRHKEAISTFEQILGKNPENTDALFHKAKSLLLVGKISESLEVLQMTVSIEPLYKQI
                     TTKDPVFAKLAANQAFKSIIK"
BASE COUNT         1677 a         1053 c         1151 g         1870 t
ORIGIN      
        1 aacaattgca gactcgacgc ctatgactcc tctatgggat tgtctgtgtc cttttctaat
       61 aaggttcatt ggctttctaa tatagcgtaa gagtatattc tagagtgtga tcaggatgat
      121 caaacatagt gcctagcaac cagatgatgg ttttatttta caaatttttc agtgctttta
      181 ataattcata gccatcattt ggaagaaaag tttcaggata gcaatttagc atatgaccat
      241 ttttaacagt atagaactcc caagaattgc agtcccaatc tgtaaaaaac tgtgcgttat
      301 cattttccca atcaaagcca tttttaatgc ttgaatctgg atatgatggc ccccgaacta
      361 aagtcataga attttttgca tggtctacaa ctatttcaga gctcaaagag tctggataca
      421 gataaacaac ttttggatga gccattagtg cctcaaattc tttttttgtg acaaattcgt
      481 tgtgtagcaa aataatttta tcaaattgat tgagaatttg ggggtttttg tcgatatcca
      541 tatcatcaat cacttggtag cctagaaatt ctaagattct aaatccgttg ccacttgcag
      601 catatgtttt ttcataagtt gtgcgaattt ggttgcttag gcatgaatca caatatcctg
      661 cgtaataatc atggattcct tcccattcgt aggcattttg agtaaaaatt ggataaacta
      721 cggctgtttt tgggcttgcc atcagttctt tgtaattctg atagtaccta agctcaggca
      781 tttttgattc tttatagtct acaaatgaat tctgggcggc aacctgagag ttttggttgt
      841 ttgttagcat aaatgccatt agcaacaaca ttgttgaagt aatgctaatc atgatcgcgt
      901 tgaagtggtt aaccaacatt agattggcct tgatccttaa ttaaaatctg gatttgttca
      961 aagtgaaaaa cattatttgt aataaaaatg gtatttcttg gaaaccgttt ggctatccaa
     1021 gatctacaac tgaggtagtt atgttaggca ccaatctctc tatggtcaga ggagagcctg
     1081 ttggaacgat gacttcggct cttataacat cgagtgcgct aggtctttca tctcctttat
     1141 gaattatagc aattatcgcg tgttcgcctt ggtcgagtat agaattgttg ttcctattta
     1201 cggcaaaata cactacagct gctgttgcgt ccgctgcagt attacttgta attggattga
     1261 cgttgatata acctgccgtt tctgccgcgt ccattgcgag tgttagattt tcatagttac
     1321 cacttgacaa aacacccgtc ataatattgt catatgatac agtgcctgtg aaatatctaa
     1381 cagccatggt agtattaccc aagtttacag atgatccacc tgatgcgact ttgactggaa
     1441 ctgctgttac gttgagatat ccaactggtg acgcatcgcc tgctgccgtg actttgcctg
     1501 caatttctag tgcgctactt gattcgtcta agcttgctac tatagcagtc tttgctcgtt
     1561 gtgttgtaga gaaacccatg ttgagaacga caaaagctaa ggctgctgct acaattacaa
     1621 atgcaatcat aacaattgca gactcgacgc ctatgactcc tctatggaat ggtcggtgtc
     1681 cttttctaac aagtttcatt ggctttctaa tatagcgtaa gagtatattc tagagtgtga
     1741 tcaggatgat caaacagatg atgtaacatg ttaacttgta ttattcaatt cattatactt
     1801 tcagatatgg ttatggtttt caataatagt atacatatta ttttataatc taatccatac
     1861 atttgggatt caaattaata aaataatgtt ttcaagtttg tattgaataa gttgattgca
     1921 attagatcaa aaaataacta ctagtctcta aataaaataa aaagaaagtt gtgattattt
     1981 gcacaatcac tttatcccat atcgactaca agattggtga tgtttggtac ctggcgttca
     2041 atagtcagtg ctgcaccagt tggtacaaca atttccgctc tgatcttgtc taatgatgca
     2101 ggtctatctg ctgtttgata ggcaatggcc atcacagcgt gctcaccttg atccagtata
     2161 gcgtttgtgt ttgttcttac agcccagtag atgatcgcct ttgtggatga tggtgttgca
     2221 ccagttattg gatgtgcact gttaaatgca tcaaaagcac taccagattc tgctgtagcc
     2281 cgtgtgaatg cctctgtagc attatcgtat acatcatcgg tgattggtcc agcatagatg
     2341 ttatcgtatt gtacggtgtt gcttagatac ttgactgaag cagtacttga gcttaagtca
     2401 attgattcac cgccagatgc gattctgagt ggaattgttg ttacgttaag taacggtgct
     2461 gtaccacatg cagtttcgct tgagccggat gtgcatccaa agcctgtgac tttgccgcca
     2521 atctctaggc tgctacttgc ttcacctaag ccagctacta ttgtagtctt tgctttctgt
     2581 gttgtagaga aacccatgtt gagaacgaca aaagctaagg ctgctgctac aattacaaat
     2641 gcaatcataa caattgcaga ctcgacgcct atgactcctc tatgggattg tctgtgtcct
     2701 tttctaataa ggttcattgg ctttctaata tagcgtaaga gtatattcta gagtgtgatc
     2761 aggatgatca aacattgaat tggtggaatc ttgattattt tttagcaaag tcatcacttg
     2821 gtgaaaaaat tttcaacttt gatgtttgtt tgaataaaac agacaacaac taaaatcatt
     2881 attatctttt tgattttaca tgatatcgat aagaaacaat tacaccactg ttgagaaaaa
     2941 aaatccaagt cctcaagtta ttttgatggg tgctacacaa tgagcgttca ggaagaacta
     3001 gttacagcac tgtcatattt tggcttggaa aaaacagacg caatggttta cctaggtttg
     3061 ttgcagatgg gctctgtcac agtaggtaca atgtctgcaa agcttgacat cgacaggggc
     3121 aaggcatata gatcattaaa caagttgcga aatcttggat tgattacaac gaccttttca
     3181 aacccaacaa tttgcaaggc catggatcca aaagaaggac taaccacaat aatgcagaga
     3241 agggaagacg agattataac aatgcaaaag ctttctcgca aaattgttga acagctgcaa
     3301 cagatcacac gacctgagac agtcacagag atctcatcat tttcaataat tcagggaagg
     3361 gcaaacatct acgcaagagt tggaaagctc attcaggaat caacaaaaac aatctacgtt
     3421 gtcaccacag ttgaagacat tcttagaatg taccatactg caattccgga gaaaataaag
     3481 atgtgcaagc aaaacaaagg cgaagtcagg gtactgactt ggaccaatag tgaaaatctg
     3541 ttgcctttga tcaacagact aaatgccaca gaaaccaggg ttggcaagct tccatccaaa
     3601 agcaggatga ttgttgagga aggaaatcag ctcatcatgt ctggctcaat aaaagaatcg
     3661 atggacctaa atgacgacaa cgattctatt atgtacacaa actcctctga gatgataaac
     3721 aacatgttta gtctgtgtac ccatctgtgg aaaaaggcaa aaccaataga attattgatg
     3781 tagttttatc caattataag aaaatcaatc ggttacttga tgattgattt gaaggcctgg
     3841 tttgcagcca gttttgcaaa gactggatcc tttgtagtta tttgtttgta gagtggttca
     3901 atactaactg tcatttggag cacctcaagt gattccgata tctttcctac caagagcagc
     3961 gactttgctt tatggaacaa ggcatcagtg ttttctggat ttttgccaag aatttgctca
     4021 aaggtggata ttgcttcttt gtgtcgtccc aagacttgga gtgattttgc cttggcagac
     4081 agcaattcca aattgtctgg ttccatatac agtgcctttg tcagaagatc taaagattct
     4141 gcatatttgc ccaagccagt aagggacaga gatttatttt tgagtgcaac aaaatgattt
     4201 gggttgtctt tgagtatttg atcataacac tctattgatt tttcatgttg gccaagtatc
     4261 gaaagtaaga ggccttcttc taacaatatt tccttgtcag aatcagtttt gttcatcata
     4321 tcataacata aaagcgcgtt ctgatagtca ccgagtattg ccatagactg ggccttgcgg
     4381 gacaaggcta aattgtgtga cgagtcaagc tgtaaaattt tatcaaaaca taaaacagct
     4441 tcttgatgtt ttccaagttt ttggaataag attccttttt ggatcaagca gctaatatga
     4501 ttaggatgaa tttgtaaaaa tctgtccaaa atttcaaggg cttgctctga tttatctaga
     4561 tttgataagc acaatccctg atagtacagt gtctctttgt attgtggttt gatttttgat
     4621 atttgttcgg tacatcgcag tgcttcttgg tattttgcac cttttatgag agccaaggac
     4681 ttggtatacc aggcatcaag attatttcgt tccaaggcta gtgctttatc ggaatataag
     4741 atagagtcct tgagcatgcc aagattaaac aacgattttg ccttgtagac agtagactcg
     4801 acatgttttg gatctacttc cagaacctca tcgaagctgg atgctgcatt atcaaacttt
     4861 ttcatattaa acaaaacttt gcctctgtga aataatgaat ccacgtcatt ttcagattta
     4921 cttaacacct gatcaaagca ccttaacgct tcttcgtcat atcccatttc ctcaaaaatt
     4981 agtcccttgc taaatagagt tggcacgtgg tcatagtttg aagccaaaat ctgatcaaag
     5041 atctgcagtg cattctgatg tttgtttagt tccattagaa ggcgaccttt gtgatagaga
     5101 acatcaagat tggcaggatc gatttctaat gccttatcaa agtagactaa tgcgtcttgg
     5161 tgtaggcctt gttgttgcaa taaaattgcc ttgccaagga ttgcgtttgt gtgattttga
     5221 tccagctcta aagccttgtc aaagaatgtt aatgcgtttg cgtgtctgcc gagctcagat
     5281 agtacgaatc ctttgtgata taacgcttct acaaaattag gttctacttc cataattttg
     5341 tctaatatta ttagagattc ttcgtagcgg ttaagattct ctaatgccaa ggctttgcca
     5401 aaaattgaat ccatgtgtcc actttgaata attaaggctc tctcaaagtg aatcaaggcc
     5461 tcatcgtgtt tgtcgagttt tgccagtgat tgggctttat ggtaaaatgc aagaaaattg
     5521 gattggtcca gctctaagac tttgtcaaaa caggttatag cgacagaata ttttccatta
     5581 tagtacagga tcactccctt ctgatagata aggttggaat cgtatgggtt gattgccaga
     5641 gcgttgtcaa agcaggctat tgcatcttcg tattttttgt ttccaaagag gatcattccc
     5701 ttttggtagt ggacctcata gtagttgggg ttgatttgga ttgccttatc a
//
LOCUS       DASWKH010000126         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_304487, whole genome shotgun sequence.
ACCESSION   DASWKH010000126 DASWKH010000000
VERSION     DASWKH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304487"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VGA92_09185"
     CDS             complement(<1..119)
                     /locus_tag="VGA92_09185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX9846615.1"
                     /translation="MSCIFCDIITKKREGHFIYEDDDHVAVLDKYPIDKGHSLV"
     gene            238..846
                     /locus_tag="VGA92_09190"
     CDS             238..846
                     /locus_tag="VGA92_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide isomerase"
                     /protein_id="HEX9846616.1"
                     /translation="MGSGEKDSKKERRESFLDKQTREKRRNMMIGLGVLAVVVIIVGF
                     ASWTFIERSEFDPMGGPAGAGPLGDEHEHASMLVRIHGDKFDFALPAYQVKSPYIHFE
                     GGDGNTVHRHASGVPLGFLFNSLKVGLNDECFIFPDKTPEHTFCTDENYSLKFYVNHE
                     NVNSILDYVLDEDDRVLISYGNQNQTQIDTQLRELDNQIILR"
     gene            909..2591
                     /gene="ilvD"
                     /locus_tag="VGA92_09195"
     CDS             909..2591
                     /gene="ilvD"
                     /locus_tag="VGA92_09195"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9846617.1"
                     /translation="MNGEISSRNVVVGTARAPHRAMYKAMGLTDADLERPFIGVSHTG
                     NEATPCNIHLGRLAQKAKEGVHDAGATPREFSTISVSDGIAMGHEGMKSSLVSREIIA
                     DSIELMMRAHQYDGLVGIAGCDKSLPGTMMAMARLNLPSVFVYGGTIMPGFYNGKQLT
                     VQDVYEAVGAYDAGQLTLEALKNIENYACPNAGSCGGMFTANTMASISEALGIALPGS
                     ASPPAEDERREKMVYDTGVAAAKLVELGVKPREILSFEAFENAITILNAIGGSTNAIL
                     HLLALAHEARIKLTYDDFERVRRKVPHIADLKPGGMYVMNDLDKVGGVPLMLKKLLDR
                     KLLHENVITVTGKTMKQNLDAISIPAPPNDNIVKSMDSPLHLSGTAVILKGSLAPDGA
                     VVKMSGVKITKFAGKAKTFDREEDAFDAVSKNQIIPGDVVVIRYEGPKGGPGMREMLG
                     VTAALVGQGLGEKVAMVTDGRFSGATRGFMIGHVAPEAFVGGNIALVKDGDEIRIDTE
                     TNKIDLMIPEDDLKRRRAEWKPRKPNYESGALAKYASLVGSAAQGAITSPIW"
     gene            complement(2772..3929)
                     /locus_tag="VGA92_09200"
     CDS             complement(2772..3929)
                     /locus_tag="VGA92_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9846618.1"
                     /translation="MTFSLTPVRGVDLIDREKELDQLVSELSKPNSRIGFSITGIRRV
                     GKTSILYETKKRLEQKGIIVIYVSVWKTLPDTLDSFMTYLFDETIHAFERKLSLKMKM
                     TELAKMGTETLLSFLKHLKLSVEADDIVYTVSYVKGEEKDLAKATLHTFSLIDKLALK
                     TKTKAVLIIDEFPSMTELKIGKKMVGSPIVKTIRTINEDYKKTALVISGSFIHTMQAV
                     ALSPNAPLYKQLINLEIKPLQKEAIADFIKKYLKKQPDQKTLEALFETTNGIPYNLQI
                     LGRELDYLQIKNLEVGTIQKATQSLIAREGDLHFKEYLHLMQSTEVKVAKIMAVFGVN
                     GPKEIADQANMQLNEVTSLLGSLMDKGIVERVDRGQYAFTDSMFKVWLGQT"
     gene            complement(3971..>4156)
                     /locus_tag="VGA92_09205"
     CDS             complement(3971..>4156)
                     /locus_tag="VGA92_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846619.1"
                     /translation="ERPKGNVLYSDLTFPRKAIQLKSSAKEAVCQICKSELREGSALT
                     ARYVDSKIVLMCGYHKF"
BASE COUNT         1263 a          811 c          913 g         1169 t
ORIGIN      
        1 actagtgagt ggcccttatc gattggatac ttgtccaaaa ccgctacgtg atcatcgtct
       61 tcatagataa aatgaccctc cctttttttc gtgatgatgt cacaaaaaat gcagctcaac
      121 acatgcaatg atagattccg caatttatac aaatgggcaa agggaataac aaatttgcag
      181 cattagacaa tctcatcgat tgattaatta gggctaattt tttagtttga ttgttttatg
      241 ggcagcggcg aaaaagactc taaaaaggaa cggcgcgaaa gttttctaga caagcagact
      301 cgcgaaaaac gaagaaacat gatgattggg ctaggagttt tggcagtagt tgtcataatt
      361 gtaggatttg catcttggac gtttattgaa agaagtgagt ttgatccgat gggtgggcca
      421 gccggagcag gaccattagg agacgagcat gagcacgcat caatgcttgt caggatccat
      481 ggcgacaagt ttgactttgc actgcctgca tatcaggtaa aaagccccta cattcacttt
      541 gaaggaggag atggaaacac cgttcacaga cacgcttcag gtgttccatt gggatttctc
      601 tttaattcat taaaagtagg actaaatgat gagtgcttta tcttcccaga caagacccca
      661 gagcacacgt tttgcaccga tgagaattat tcactcaagt tctacgtcaa ccacgaaaat
      721 gtgaatagca tattagatta tgtgctagac gaggatgaca gagttttaat ctcatatggt
      781 aatcaaaacc aaacacaaat tgacacccag ctccgagagt tggacaacca aataatcctg
      841 cggtagattc tagcgcatga gaattgaaaa aattctaata tgttactcga cctaagccca
      901 gtgatactat gaatggagag atttcaagca gaaatgtagt tgtcggcacc gcaagagcac
      961 cccatagggc aatgtacaag gcaatgggac taacagatgc agacttggag aggccgttta
     1021 tcggagtatc acacactgga aacgaggcaa cgccatgcaa tattcacctt ggaagactag
     1081 cacaaaaggc aaaggagggg gtgcatgatg ccggggcaac accgcgagaa tttagcacca
     1141 tttcagtaag cgatggaatc gcaatgggtc atgagggaat gaaatcatcg cttgtcagtc
     1201 gcgaaattat tgcagactcg attgaattga tgatgagggc tcaccagtat gacgggctag
     1261 ttggaattgc gggatgtgat aagagtttgc ctggtacgat gatggcaatg gcaagactca
     1321 atctgccgtc ggtgtttgtg tacggcggaa cgataatgcc gggattttac aatggaaaac
     1381 agcttacggt tcaagatgtc tacgaggcag taggcgcata tgatgcagga cagctaacac
     1441 ttgaagcact caaaaacatt gagaactatg catgtccaaa tgccggctcc tgtggcggaa
     1501 tgtttactgc aaacacgatg gcgtcgattt ctgaagcgct cggaattgca ttgccgggaa
     1561 gtgcaagccc gccagcagaa gatgagcgaa gagagaaaat ggtctacgac acaggtgttg
     1621 cagcagcaaa gctagttgag cttggagtaa aaccaagaga gattttgagt tttgaggcat
     1681 ttgaaaatgc aattacaatt ctaaatgcaa tcggcggctc gacaaacgcg attttgcatc
     1741 ttctagcact tgctcatgag gcaagaataa agctaaccta tgatgatttt gagagagtac
     1801 gaagaaaggt gccccatatt gccgatttga agcctggggg aatgtacgtc atgaatgacc
     1861 ttgacaaagt aggaggagtt ccactgatgc tcaaaaagct tcttgacaga aagctactcc
     1921 atgaaaatgt catcaccgtt actggaaaaa cgatgaagca gaaccttgac gctatatcca
     1981 taccagcccc gccaaacgat aacatagtaa agtcaatgga ctcgccattg cacctatctg
     2041 ggacggcagt aatactcaaa ggctcgcttg ccccagatgg agcagtagta aagatgtctg
     2101 gcgtaaaaat taccaaattt gcaggaaagg caaagacctt cgatagagaa gaggatgcgt
     2161 ttgacgcagt atcaaaaaac cagatcatac cgggtgatgt agtagtgatt agatacgagg
     2221 ggccaaaagg aggacctggt atgagggaaa tgcttggagt aactgcagct cttgtaggac
     2281 aaggtcttgg tgaaaaggta gcaatggtaa cggatggcag gttttctggc gcaacacgcg
     2341 gatttatgat tggccatgtg gcgccagagg cattcgttgg tggaaatatc gcacttgtaa
     2401 aagacggcga tgagataaga atcgatacag aaacaaacaa aattgatcta atgattccag
     2461 aagacgactt aaagcgcaga cgagccgaat ggaaacctcg aaaaccaaac tatgagagtg
     2521 gggccttggc aaaatatgca tcacttgtag gatctgctgc acagggtgca attacaagtc
     2581 ctatttggta aatggatcaa atgggattcg aatccaaaaa taacacataa cgtatctttg
     2641 ggttctgcaa aacgacatta catcacaatt ttttgttgat ttttctacga attgactcag
     2701 cacttgacaa agcagagctt ttggattttt taccaacaac aataacttta tagactataa
     2761 ctttattgtc ttcaagtttg tcctagccac actttaaaca tgctatcagt gaatgcatac
     2821 tgacctctat ccacacgttc aactatgcct ttgtccatga ggctaccaag aagagaagtg
     2881 acctcattta gctgcatgtt tgcctggtct gctatctctt ttggaccatt tacaccaaaa
     2941 acggccatga ttttggctac tttaacctca gttgattgca tcaaatgcag atattcttta
     3001 aagtgaaggt cgccctccct cgcaatcaag ctttgtgttg ctttttgtat tgtaccaacc
     3061 tcaagatttt ttatctgcag atagtcaagc tctctgccta atatttgcag attatatgga
     3121 atgccgtttg ttgtctcaaa caacgcttca agtgttttct gatcaggttg tttcttgaga
     3181 tattttttta taaaatcagc aattgcctct ttttgcaatg gttttatttc caaattgatt
     3241 agttgtttgt acagcggagc atttggggat aatgcaactg cttgcatagt atgaataaaa
     3301 gatcctgaaa tcaccaaagc tgttttttta taatcttcat ttatggttct aatagttttt
     3361 acaatgggcg aaccaaccat tttttttcca attttcaatt cggtcattga tggaaactca
     3421 tcaattataa gaacagcttt ggtttttgtt ttcaaagcca gcttatcaat caaactgaat
     3481 gtatgtaaag tggcttttgc aagatctttt tcttctcctt ttacatatga tactgtatat
     3541 acaatatcat cagcttcgac agaaagtttg agatgcttta aaaaactcaa caatgtctcc
     3601 gtccccattt tagcaagctc ggtcattttc atctttaagg agagttttct ctcaaatgca
     3661 tgaattgttt catcaaaaag atatgtcata aaagaatcaa gcgtatctgg cagggtcttc
     3721 caaactgaga catatattac gataattcct ttttgctcaa gtctcttctt tgtttcatat
     3781 agaatacttg tttttcccac tctgcgaata ccagttatgg agaatcctat tctagagttt
     3841 ggcttgctaa gctctgagac gagctgatct agttcttttt ctctgtctat tagatcaact
     3901 ccccgtactg gtgtaagtga aaaagtcacg gttaaactat aaagatatag actataaagt
     3961 tattgcttaa ttagaatttg tggtaaccac acattaagac aatcttacta tcaacatatc
     4021 tggcagtaag tgcgcttcct tcccttagct cggatttgca tatctgacat actgcctctt
     4081 tagcagagct ttttagctga attgcctttc ttggaaaggt aagatccgag tacagcacgt
     4141 tgccttttgg cctctc
//
LOCUS       DASWKH010000127        15362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1819898, whole genome shotgun sequence.
ACCESSION   DASWKH010000127 DASWKH010000000
VERSION     DASWKH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 15362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15362
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819898"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VGA92_09210"
     rRNA            complement(<1..807)
                     /locus_tag="VGA92_09210"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            1088..2398
                     /locus_tag="VGA92_09215"
     CDS             1088..2398
                     /locus_tag="VGA92_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9846620.1"
                     /translation="MDDFEKLYKKKTPQSAKLFEKSKRQHVSGVSHNIRFFEPYPFVT
                     KSARGKFLVDVDSNKYVDFWMGHWSLILGHVPKQVFDKVREQLRNGWMYGVVNKNTLE
                     LSDLISKAVSVAQKIRYATSGTEADMYAVRLARSATGRKTIAKIDGGWHGYTSDLLKT
                     VNWPFDQSESSGLTDEEHVVSLPYNDLEKSIAILRSVKKDLAGVIVEPVLGGGGCIPA
                     TKEYLHGLQEFVQKENALFILDEIVTGFRFRYGCLYSMLNLDPDIVTLGKIAGGGFPI
                     GVICGKDEVMKFANTGLYPKTKRAYIGGGTFSANPMSMSAGAATLSVLKNNNMVYSKI
                     KRLGERARKNLDKVFDGKVITTGKGSLFMTHFITDGITQIQNSEDVSRCDSELLHRYH
                     FEMIAKDGIFFLPRKLGAFSSAHSDADVKSLIDASERFAHRIHR"
     gene            2429..2788
                     /locus_tag="VGA92_09220"
     CDS             2429..2788
                     /locus_tag="VGA92_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9846621.1"
                     /translation="MLIADLDSSQPIVGSEGTLIKQIFHPHNTMLGIRYSMVQCRIEP
                     GKNSKLHKLKSSEVYFVTSGEGVLHIDDESCHVKKNQAIYVPPHSKQFIQNIGKAPLE
                     FLCIVDPAWRQEDEIVG"
     gene            2845..3489
                     /locus_tag="VGA92_09225"
     CDS             2845..3489
                     /locus_tag="VGA92_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846622.1"
                     /translation="MTELDGFLFKSILHAIRNQLNTEKIGDIEQKLQQEHGLKFSDLF
                     NRFDEMKNSLFEFESELKKIEDGILRDFLTVETAGTTSEKWLVIKNKYLTELILKTFA
                     DEDKKAILDLTRDSVETIPKILVLCNLPNTSGYRKMNQLIEDGFVTPIGLAETFEGKR
                     AILYKSIIQKIQIIINRNVIITKILIPKEIVNSSQIVRTITEVIQGKANPSFAN"
     gene            3526..4218
                     /locus_tag="VGA92_09230"
     CDS             3526..4218
                     /locus_tag="VGA92_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9846623.1"
                     /translation="MNASQAYEVLHLQRNSSFNEIKYAYRKLALELHPDKNNIEKDGS
                     KFKLVTEAYHVLKHNNKVTNAQFREPTPDKKYADTKTKKEKTFSRTSWGARPDDGPPQ
                     EDWTRYTKQTEQSDPFFWKSYVAEFWKSYEAKNNQAKNPYDFEVTREVKKEPDLSVDV
                     DHSRCIGCCSCETIAPQVFSVDKITKMNPKSNVINRKGAKMDKIMDAAQTCPTKAINV
                     EEKETGRRLYPY"
     gene            complement(4215..4622)
                     /locus_tag="VGA92_09235"
     CDS             complement(4215..4622)
                     /locus_tag="VGA92_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9846624.1"
                     /translation="MDCIFCKIAKGEIMARIIMATKNSLAFLDAFPVAKGHALVIPKN
                     HYEKIQDMSPDDNKDVFDTVYKVVKKVDKLTGSTLVAIHNGKGAGQEIPHVHIHLIPR
                     STDDSAGAVHSMFKNRPKISDSEFDQILGKLKD"
     gene            4728..5876
                     /locus_tag="VGA92_09240"
     CDS             4728..5876
                     /locus_tag="VGA92_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010195448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9846625.1"
                     /translation="MSIKNITVLGSGVMGHGIAQVSAMAGYNIVLRDVDQQFLDKAME
                     KIKWSLEKLVTKEKITQAESDAILGRIKTKVELKDAVQSADLIIEAVPEIMELKKKVY
                     AELDSVADKKTVFASNTSTLPITEIANTTSRPERFIGIHFFNPPQLMKLVEVIPGQKT
                     SQDVLDLTVEFVRSVKKEPVVCKKDVPGFIVNRLFIPMVHEACWLKQRQNLKLEEIDS
                     AVKFRMGFPMGIFELADFTGMDVIHKATIELQIRDKKVIFPHPEIEKLFNEKKLGQKS
                     GEGFYKYSDDKYERIQLDEELAKKCNPIQLVANILNNAAWLVTNKASDIAEIEKAAQL
                     GLGLKKPIFETAREFGIKNIVNELNDLAKKYGTFYEPDPLVTSMQKSG"
     gene            complement(5857..6366)
                     /locus_tag="VGA92_09245"
     CDS             complement(5857..6366)
                     /locus_tag="VGA92_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HEX9846626.1"
                     /translation="MSKRRQILVIGNNENGCTPELEKTAYDVGQEVAKSGSVLISGGL
                     GGVMRGACHGARDAGGLTVGIIPQNDPSFANEFCDIVIPSGLGLARDFLTALSADGVI
                     VVGGGSGTLSEVCAAYMHKKPITALKNSGGIASQYTDQFLDHRQNVKIVGVSTPKEAV
                     TYILSQISA"
     gene            6466..6588
                     /locus_tag="VGA92_09250"
     CDS             6466..6588
                     /locus_tag="VGA92_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846627.1"
                     /translation="MSKRTMPVCLTAEQYKKIEKIAKKHGLLNASQAIEKVLMD"
     gene            6652..7062
                     /locus_tag="VGA92_09255"
     CDS             6652..7062
                     /locus_tag="VGA92_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846628.1"
                     /translation="MGLFSKKPVNCTVCNKETVHKHKPKKEWNLQYPLCTECYMKTME
                     QYYSGSFKQKCVSCGVVQKLTDLWEPRWQWEMEGLLCKSCFDTKEVDFSQKKEFCSIC
                     GAKLNFFRYNPKGSWKVQGQLCRKCWDDQKEQHK"
     gene            7125..7250
                     /locus_tag="VGA92_09260"
     CDS             7125..7250
                     /locus_tag="VGA92_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846629.1"
                     /translation="MQHQQIVHGKDLPYDCKICNQNFSNMQDMRTHLQRYHSYKN"
     gene            complement(7247..7477)
                     /locus_tag="VGA92_09265"
     CDS             complement(7247..7477)
                     /locus_tag="VGA92_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HEX9846630.1"
                     /translation="MSEEPLETPTTPSELETPTTPSEPATEKFDVIYKCLRCGTTVTN
                     TELSRLPEIKCICGFRVFNKVRPPIVKTVSAI"
     gene            complement(7513..7821)
                     /gene="rpsJ"
                     /locus_tag="VGA92_09270"
     CDS             complement(7513..7821)
                     /gene="rpsJ"
                     /locus_tag="VGA92_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX9846631.1"
                     /translation="MTQSARIKLTSTSLPKLDGVCTEILGIGQKTGVRVKGPTPLPVK
                     RLNVVTRKSPCGNGTETYEKWEMRMHRRIIDLSADDKAIRQLMRLKIPDDVYIELSLK
                     "
     gene            complement(7834..9132)
                     /gene="tuf"
                     /locus_tag="VGA92_09275"
     CDS             complement(7834..9132)
                     /gene="tuf"
                     /locus_tag="VGA92_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HEX9846632.1"
                     /translation="MATKPHLNLIVTGHIDNGKSTTMGHFLMDMGLVDERTIASHAAE
                     SEKTGKGDTFKYAWVMDNIKDERERGITIDLAFQKFETPKYFFTLIDAPGHRDFIKNM
                     ITGASEADAAILVLSAKEGETDTAIAPGGQAREHAFLLKTLGVNQLIVAINKMDDSNY
                     SEVAFKAAKEKAEKLIKSVGYKVENVPILPISGWKGDNLVKKSENMPWWTGKTLLLAF
                     DDFTLPEKPVGKPLRLPIQDVYSITGVGTVPVGRVETGKFKAGDKIVIMPSGAIGEVK
                     SIETHHTQMESAEAGDNIGFNLRGIDKKDVKRGDVIGHPDNPPKVAKEFRAQIIVIHH
                     PTAIAPGYTPVMHAHTTQVAATITEFEAKINPATGAVDEQNPKFLKVGDSAIVKIRPV
                     RPTCIETFKDFPEMGRFALRDMGATIAAGIVKDITEEHKP"
     gene            9367..10497
                     /locus_tag="VGA92_09280"
     CDS             9367..10497
                     /locus_tag="VGA92_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEX9846633.1"
                     /translation="MKVTVSAIKADVGGIGGHTRPSDGLLDAVRRTIKSAGSLLLDHY
                     IGYAGDDVHIVMSHTKGVDNSEIHGLAWKAFEEGARVAKAEGLYGAGQDLLKDSFSGN
                     VKGMGPGVAELEFEERPNEAFTVLAADKTEPGAFNYPFYRLFVDALSNTGLIVNKSLA
                     AGVKFNIMDVEKGEIAELVLWEDKPILEAALMYPGRYVVSSVYTKSGEPIVASSTDRL
                     HNIAGTYVGKDDPIAVVRTQKNFPATEELGSVFNNPHYVAGNTRGSHHMPLMPVKINT
                     AATIDFCIPLIEALVFSMHDGKFTGPFDGFSTPDWDYVRQIATQKALAMRSQGFIHPA
                     TLVPAELEYAEGYKAVIDGLNKKMKPLEKKNIHGSKKYEDPD"
     gene            10553..10957
                     /locus_tag="VGA92_09285"
     CDS             10553..10957
                     /locus_tag="VGA92_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846634.1"
                     /translation="MRFNLIEYLRSVIFGIGIVSVSHIFLTIFGQTIPNAVVGFFREV
                     GGVLILAVVCAFAFTWFLRARPHHKPKKYSVVVFDVFGQQTNIEGLRTDFRNHDVAWS
                     FMKQYKNDYPTHNFAMVADNSDSAKKTIFRYI"
     gene            11004..11297
                     /locus_tag="VGA92_09290"
     CDS             11004..11297
                     /locus_tag="VGA92_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846635.1"
                     /translation="MPLDVIQEKSLTEIINYALEYPSMVLSSTKSNHIVPTSLADFAY
                     GLYVGYISGMFFDKFLAENKRFLNDDELVDFYSKLASRNSEITLKIKAHLKTQ"
     gene            11346..13112
                     /locus_tag="VGA92_09295"
     CDS             11346..13112
                     /locus_tag="VGA92_09295"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9846636.1"
                     /translation="MKFSLISETFEKMEATTKRLELTQHLVDLFKQTPPEIIAKVVYL
                     IQGKIRPDFEGVELGVAEKLAIRALSKSAGIPIKKIAEIYAEDGDLGHTAAKMIERKT
                     QTTFLTQDITAERVYETLYKIAKLEGARSQDMKMKYISSLLNDATPQEAKFILKISMG
                     TLRLGIADNTVMDALAIAYTGAKENREKLEAAYNVSSDLGRVAEAIANNNLAGLENFE
                     VTLFSPIRPMLAERVKSEREAVEKLGKEFVAEYKLDGERVQIHINNEKVVLFSRRLEN
                     ITTHYPDIVENIPKALKTTEAILEAEAVAINDNTGEFLPFQELMHRRRKYDIAKTVEQ
                     YPITVNLFDLMYLNGRDLLNSTYEERRKRLEEITTENDFVRLVPRTIVRSENEIEDFL
                     ENAINAGCEGLMLKVLDAPYRAGARGSNWLKLKREYRNELGDSLDLVVIGAFFGRGRR
                     TGKYGTLLLATYNQEDDTFDSICKVGTGFTDENLDQFYQILSDKVTLKKNPRIDSGME
                     ADVWFEPELVIEIVASEITLSPIHRTAKDVIRKGSGLALRFPKFTGKIRTEKAPEDAS
                     TDQEVITLYRDQKKVTQGVSES"
     gene            13155..13808
                     /locus_tag="VGA92_09300"
     CDS             13155..13808
                     /locus_tag="VGA92_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX9846637.1"
                     /translation="MYSGELEVQAKRKAIAVLQDEINRILNAARELATLPALVMKKDK
                     VGIKAVNEQISSIEEEVENLRRKITREVADVGGLIMNRENLLNTAYTMDEIAGYITGI
                     AFKLSQIKPTTLKNAKMDVDISALIDLVVDQVYKLNEIVRGLNTNTANAIELAQETQK
                     IEREIDIKYRTTVVKALDEISNTKELMLVKDVIQGIEEMADKCQQVSDSFILLALSL"
     gene            13872..14405
                     /gene="endA"
                     /locus_tag="VGA92_09305"
     CDS             13872..14405
                     /gene="endA"
                     /locus_tag="VGA92_09305"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HEX9846638.1"
                     /translation="MQGELVENRIIVWSTKDARSLFTDSYYGKPIGIPKPKPEEINVP
                     LILDLIEGHYLQEKGEITIFKSKKKITRQQLLQICRNEYHNFDKKYLVYKDFREKKYI
                     VNPGIKFGCDFAVYQRGPGVDHAPYLVQVYNKNDTLSSTGVVLAGRLATSVKKNFILA
                     IPHGPKVNYLALDWWKA"
     gene            complement(14402..14773)
                     /locus_tag="VGA92_09310"
     CDS             complement(14402..14773)
                     /locus_tag="VGA92_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846639.1"
                     /translation="MQDPTISHWGAQDKFQAHFIVRMDAARPEKYVAKTILKTEGHFG
                     AKKVKSVSWNGGLLAEELNKDTELNELIAKQPLKDATIFVDPAVKEIRIYNKWKNAYD
                     FKITKDLYVIYDKIAEHVKKI"
     gene            complement(14779..15018)
                     /locus_tag="VGA92_09315"
     CDS             complement(14779..15018)
                     /locus_tag="VGA92_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846640.1"
                     /translation="MHDTESDTFVYETWPEKVSHMLKEIGLDATPKELGTDPVENAKD
                     YYGRNFSQATRMITNRGCVELKNSNIDVIQIIQKG"
     gene            15102..>15362
                     /locus_tag="VGA92_09320"
     CDS             15102..>15362
                     /locus_tag="VGA92_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010190084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9846641.1"
                     /translation="MLKFQNKIVLVTGSGTGIGQTVAKLFAENGASVIILGRRKEPLE
                     ETKKILDDIINNAKSNAKVWLFSGVDVSDEEGVSQMFDSLKQS"
BASE COUNT         4839 a         3099 c         3235 g         4189 t
ORIGIN      
        1 cggcttccct gcggcactgc actggccata agccaatgca tcaccgagtc tgcatcgttt
       61 acagctggga ctacccgggt atctaatccg gtttgctccc ccagctttca tccctcaccg
      121 tcgaacgtgt tctggtacac cgccttcgcc acaggtggtc atcaatagat caaaggattt
      181 taccccttcc tatcgagtac cgtgtacctc tcccactccc tagctgtcca gtattttcgg
      241 cagcctatac gttaagcgca tagatttaac cgaaaactta aacaaccggc tacggatgct
      301 ttaggcccaa taaacctcct gaccactcga ggtgctggtt ttaccgcggc ggctgacacc
      361 agaacttgcc caccccttat tcaccagtgg ttctatgact gacaaaagac tcctttagca
      421 ggagacactc ggattaacct tgtcgtgctt tcgcacattg caaaggtttc tcgcctgctg
      481 cgccccatag ggcctgggtc cgtgtctcag tacccatctc cgggcccctc ctctcagagc
      541 ccgtacctgt tatagccttg gtgggccatt acctcaccaa ctagctgata ggccgcagtc
      601 ccatcctacg gcgataaatc atttcaacta cgaaccattc caggtgccgt aatctatcgg
      661 gtattattct cagtttcccg aggttatccc cgtccatagg ttaggttaac tacgtgttac
      721 tgagcagtcc gccttgtatt gctacaacga ctcgcatggc ttagtaccaa tccgatagca
      781 gtcaggtccc gcaggatcaa cgggattcat tgtttggttt attcatattt ggaattggac
      841 ggaatgcact aatcttcaca tagtcagatt agatcgtttt gatcaaatcc tcattttgta
      901 tgcgtaactg gaggcctgta aatcacaaac tggtatgata ccatacttca tcacaagcgc
      961 cgccaaagcg ttacgtatgc aaaagagaaa caatatgaga tggcatataa accatgcaga
     1021 attttttaca aattacatga ttttttcgat caaaaatata tggcgtaata taaagtagca
     1081 aatacacttg gacgattttg aaaaactcta caaaaaaaag accccacagt ctgcaaagct
     1141 ttttgaaaaa tcaaaaagac agcacgtaag cggtgtaagt cataacatca gattttttga
     1201 gccgtatcca tttgtgacaa aatctgctag gggaaaattc ctcgttgatg ttgactcgaa
     1261 caaatacgtt gatttttgga tgggccattg gagtctgatt ttagggcatg tgccaaaaca
     1321 ggtctttgac aaagtaagag aacagttacg aaacggctgg atgtatggag tagtaaataa
     1381 aaacacgctg gagctttcgg atctaatttc aaaagcagtt tctgttgcac aaaaaatccg
     1441 atatgccaca tctggaactg aagctgacat gtatgcagtt cgactcgctc gttctgcgac
     1501 tggtagaaaa accattgcaa aaatagatgg tgggtggcac ggctacacaa gtgatctgct
     1561 aaagactgta aattggccat ttgatcaatc agaaagcagc ggcctcaccg atgaggagca
     1621 cgtagtatca ttgccgtaca atgatttaga aaaatcaatt gcaattctaa gatcagtaaa
     1681 aaaagacctt gcaggagtaa ttgttgagcc agtcctaggt ggtggaggct gcatccctgc
     1741 aacaaaggaa tacctgcacg gcctgcagga atttgtgcaa aaggaaaatg cactattcat
     1801 tttagatgag atagtcaccg gctttaggtt caggtatggc tgcctgtaca gcatgctaaa
     1861 tcttgatcca gacattgtca cactgggaaa aatcgctggt ggcggatttc caataggcgt
     1921 aatctgtggg aaagatgaag tcatgaaatt tgcaaacact gggctttatc caaaaacaaa
     1981 acgcgcatac atcggtggag gaacattttc tgcaaatccc atgtcgatgt cagcaggtgc
     2041 tgccactctt agtgtcctaa agaacaacaa catggtttat tcaaaaatta aaaggcttgg
     2101 agaaagggca agaaaaaatc ttgacaaagt ctttgatggt aaagtaataa caactggcaa
     2161 gggctcattg tttatgactc attttattac cgatggcatt acacaaatac aaaattcaga
     2221 agatgtctcg aggtgtgaca gcgaattatt gcacagatat cattttgaga tgattgcaaa
     2281 agacggaata tttttcctac caagaaaact gggtgcgttt tctagcgccc attctgacgc
     2341 agatgtcaaa tcactaattg acgcatcaga gagatttgct catagaattc acagataaag
     2401 aaaaataaaa cacaaaatag gtagtgttat gttaattgct gatcttgact catcacaacc
     2461 aatagttggc tccgaaggga cactaatcaa acaaattttc cacccacaca acacaatgct
     2521 tgggattagg tacagcatgg tgcagtgcag aatcgagcca ggcaagaatt ccaagctaca
     2581 caaactaaaa tcatctgaag tttattttgt cactagtggt gagggagtct tgcacataga
     2641 tgatgagtct tgccacgtta aaaaaaacca ggcaatttat gtgccaccac attcaaaaca
     2701 atttattcaa aacattggaa aggctccact ggagtttttg tgcatagttg atccggcttg
     2761 gcggcaagaa gacgagatag ttggataacc atgttttgaa aatacagcgc ctgacttaaa
     2821 taacaaaaac cgcttatcgt gcttatgacc gagttagacg gatttctttt caaatcaata
     2881 ttgcatgcaa taagaaatca gctaaatact gaaaaaattg gagacattga gcaaaaacta
     2941 cagcaggaac atggtttgaa gttttctgac ctctttaaca gatttgatga gatgaaaaac
     3001 tcgttatttg agtttgaatc cgaactaaaa aagatcgaag atgggatact aagagatttc
     3061 ctcactgtcg agacagccgg cactacgtca gaaaaatggc tagtcattaa gaacaaatac
     3121 ctaactgaac ttatcttaaa gacgtttgca gacgaagaca aaaaagcaat tttagatctt
     3181 acaagggaca gcgtggaaac aattccaaaa attttagttt tgtgtaactt gccaaacact
     3241 tctggctata gaaaaatgaa tcagctaata gaagacgggt ttgtaacgcc aattggatta
     3301 gctgagacct ttgagggaaa acgcgcgata ctttacaaat ccatcataca aaaaatccag
     3361 atcatcataa acagaaatgt aatcattacc aaaatcctaa ttccaaaaga aattgtcaat
     3421 tcaagccaga tagtaaggac gataacagag gtaattcaag gcaaagcaaa tccttctttt
     3481 gcaaattaag catattttta tcatagtttg cccaatattg gactattgaa tgcatctcag
     3541 gcctatgagg ttttacacct acaacgtaac tcatcattta atgagattaa atatgcatat
     3601 agaaaactgg cactagaact acatccagac aaaaacaata ttgaaaaaga tggatcaaaa
     3661 ttcaaacttg taactgaagc atatcacgtg ctaaaacaca acaataaagt tacgaatgcc
     3721 caattcagag agcccacacc agacaaaaaa tatgctgaca caaaaacaaa aaaagaaaaa
     3781 acattttcac gcacaagctg gggcgccaga ccagacgatg ggcccccaca ggaggactgg
     3841 acgagataca caaaacaaac agagcaatcg gatcccttct tttggaaaag ctacgttgca
     3901 gaattttgga aaagctatga ggcaaaaaac aatcaggcca agaatccgta tgattttgag
     3961 gttactcgag aagtaaaaaa agagccggat ctttctgtcg acgtagacca cagtcgctgc
     4021 attgggtgtt gtagctgcga gaccatagcc ccacaggtct tttcagttga caagatcaca
     4081 aagatgaacc ctaaatcaaa tgtgataaat cgcaaaggcg caaaaatgga caaaataatg
     4141 gatgccgcgc agacctgccc aactaaggca atcaacgtgg aagaaaaaga gaccggcagg
     4201 cgcctgtatc cctactagtc ctttagtttc cctagtattt gatcaaattc ggaatctgag
     4261 atctttggcc ggtttttgaa catgctatgc actgcaccag cggagtcatc agtgcttcga
     4321 ggtatcaaat gaatgtgaac gtgtggtatc tcctggcccg ctcccttgcc attatgaatg
     4381 gcaacaagag tagagcccgt caacttgtct acttttttta caactttgta tactgtatca
     4441 aagacgtcct tgttatcatc aggggacatg tcctgaattt tttcatagtg gttttttgga
     4501 attacaagtg cgtgtccttt ggctacagga aaggcatcta agaatgcaag tgagtttttt
     4561 gtcgccataa ttattctggc catgatttca cccttggcta tcttgcaaaa aatacaatcc
     4621 acaattgctc atcatcagtg tctttgatat atttttcagt ttgactttgt tggttttgaa
     4681 aaataaattg aaatagatta aaaaggatag cgagcaaccc aagagagttg tcaataaaaa
     4741 acataacagt tctagggtca ggcgttatgg gtcatggcat tgcacaggta agcgcaatgg
     4801 ctggatacaa catagttctc agagatgttg accagcaatt cctagataag gcaatggaga
     4861 aaataaaatg gagtcttgag aaacttgtta caaaagaaaa aatcacgcag gcagaatccg
     4921 acgcaatact tggacggatc aaaaccaaag tagaactcaa agatgcagtc cagtctgcag
     4981 atttgataat tgaggcagta ccggagatca tggagctaaa aaagaaggtt tatgcagaat
     5041 tagattctgt tgccgataaa aaaacggttt ttgcatcaaa taccagcacc ctcccaatta
     5101 ccgagattgc caacaccact agcaggcctg aaagattcat tggaattcac tttttcaacc
     5161 caccacaatt aatgaagtta gttgaagtca ttcctggaca aaaaacatct caggacgtgc
     5221 ttgatctgac agttgaattc gtaagatcgg taaaaaaaga gcccgtagtc tgcaaaaaag
     5281 atgtgccggg atttattgtg aatcgccttt tcatccccat ggttcatgaa gcctgctggc
     5341 taaaacaaag acaaaacctc aaactagaag aaattgattc tgcggtaaaa ttcaggatgg
     5401 ggtttccaat gggaattttt gagcttgcag actttactgg tatggatgta atccacaaag
     5461 ccacaataga actgcagata cgtgataaaa aagtcatatt cccacaccca gaaatagaga
     5521 agcttttcaa tgaaaaaaaa ctaggacaaa agtcaggtga gggtttttac aagtattctg
     5581 atgacaagta cgagcgaata cagcttgatg aagaattggc aaaaaaatgt aacccaattc
     5641 agcttgttgc aaatattttg aacaatgctg cttggcttgt gacaaacaag gcaagcgaca
     5701 ttgcggaaat tgaaaaggcc gcgcagcttg ggcttggcct caaaaagcca atttttgaga
     5761 cggcaagaga gtttggcatc aaaaacattg taaatgagct aaacgacttg gccaaaaaat
     5821 atggcacatt ttatgagcca gatccattag ttacatctat gcagaaatct ggctgagaat
     5881 gtatgttact gcttcttttg gcgtagagac gccgacaatt ttgacgtttt gtctgtgatc
     5941 aagaaactgg tctgtgtatt gtgaggcaat accaccagag ttttttagtg cagttattgg
     6001 ttttttgtgc atgtatgctg cacaaacttc tgatagagtt cccgagcctc cccccacaac
     6061 tattacgccg tcggctgaca gcgcagtcaa aaaatccctc gccagtccta ggccgcttgg
     6121 tatgacaata tcacaaaact catttgcaaa tgagggatca ttttgaggaa ttattccaac
     6181 tgtaagccca ccggcatcac gtgcgccatg gcacgcacct ctcataaccc caccgagtcc
     6241 gccacttatt aaaacagagc cagactttgc aacctcctgg cctacatcat atgcggtttt
     6301 ttctagttct ggcgtgcagc cgttttcatt gttgccgata acaagaattt gccttctttt
     6361 tgacatatat tatacatgtt tttttcgcac agatatttct tggtatctgt aaattggatc
     6421 gattgtgagc aaaaaggata taaaattaga atcattactc aaaccatgtc aaaacgaaca
     6481 atgccagttt gtctaacagc agagcagtac aaaaaaattg aaaaaatcgc gaaaaagcac
     6541 ggactgctca acgcaagcca agcaattgaa aaagtcctaa tggactagac ataacctttc
     6601 aacccttttt gtctcattat gtttttaaaa agactgcaat aatgcattac aatgggcctt
     6661 tttagcaaaa aaccagtaaa ttgcacagtt tgtaacaagg agacagtaca caaacacaag
     6721 ccaaaaaaag aatggaatct tcaatatccg ctctgcacag agtgctacat gaaaacaatg
     6781 gagcagtact atagcggctc atttaagcaa aaatgcgtaa gctgtggagt tgttcaaaag
     6841 ctcaccgatc tctgggagcc gaggtggcaa tgggagatgg aggggctgct ctgcaagtcc
     6901 tgctttgata ccaaagaagt tgactttagc caaaaaaaag agttttgtag catttgtgga
     6961 gccaagttaa actttttcag atacaaccca aagggaagct ggaaggttca gggccagctt
     7021 tgcagaaagt gttgggatga ccaaaaggaa caacataaat gaagtttttt aaaaagatca
     7081 agtgtgaaaa atgcgatcgt gatttcccat cagaggaaaa cttgatgcag caccagcaaa
     7141 tagttcacgg aaaggatctt ccctatgatt gtaagatatg caaccagaat ttttcaaaca
     7201 tgcaagatat gagaacacat ctacagcgat accacagcta caaaaactag attgctgaaa
     7261 ctgtcttgac tattggtggt ctgaccttgt taaaaactct aaaaccacag atgcatttta
     7321 tctctggcag tctgctcaac tcggtatttg ttacagttgt tccacaccta agacatttgt
     7381 aaatgacgtc aaatttctca gttgctggtt cagatggtgt tgttggagtt tctaattcag
     7441 atggtgttgt tggagtttct agtggttctt cagacatcga ttgttacact tttcttctat
     7501 aatttaaggc tattatttca gagataattc aatatagaca tcgtcgggaa ttttaagacg
     7561 cattagttgc cttattgcct tgtcatctgc actcagatca attattcttc ggtgcattcg
     7621 catctcccac ttttcgtatg tttctgttcc gttcccacat ggggattttc gagttacgac
     7681 gttaagcctc tttacaggaa gtggtgttgg cccctttact ctaacgccag ttttttgccc
     7741 aatgcccaaa atttctgtac atacaccgtc tagttttgga agacttgtgc tggttagttt
     7801 gatgcgagca gattgggtca tctaaaaatc tactcagggt ttgtgctctt cggtgatatc
     7861 tttgacaata cctgctgcaa ttgttgcacc catatctctt aatgcaaatc ttcccatttc
     7921 tggaaagtct ttgaatgttt caatacatgt tggcctaaca ggtctgattt ttacaattgc
     7981 cgagtcgcca actttgagga attttggatt ttgttcatca actgcacctg ttgctggatt
     8041 tatctttgcc tcaaattctg tgatggttgc agcaacttgt gttgtgtgtg catgcatgac
     8101 tggtgtgtag ccaggtgcaa ttgcagttgg atggtgaatt acaatgattt gtgctctaaa
     8161 ttcttttgcg acctttggag gattgtctgg gtgtccgatt acatcgcctc ttttgacatc
     8221 tttcttgtct atgcctctta ggttgaatcc gatgttatca cctgcttctg ctgattccat
     8281 ttgtgtatgg tgagtttcaa ttgattttac ttcaccgatt gctcctgatg gcatgatgac
     8341 aattttgtca cctgctttga atttgcctgt ctcgactctt cccaccggca ctgttccgac
     8401 gccggtgatt gaatagacat cttgaattgg taatcttaat ggcttgccaa ctggtttttc
     8461 tggtaatgta aagtcatcaa atgccagtag tagtgtcttg ccagtccacc acggcatgtt
     8521 ttcagatttt tttactaggt tgtcgccctt ccatccagaa attggaagga ttggaacatt
     8581 ctctactttg taacctactg acttgattag tttctctgct ttttcttttg ctgccttgaa
     8641 ggctacctct gaataattac tgtcgtccat tttgttaatt gcaacaatta gttggttcac
     8701 accaagtgtt ttgagcaaga atgcgtgttc tcttgcttgt ccgcccggtg caatcgcagt
     8761 atctgtctca ccttcttttg cagagaggac cagaattgct gcgtctgcct ctgatgctcc
     8821 agtaatcatg tttttgataa agtctctgtg tcctggtgca tcaattaatg tgaaaaagta
     8881 ctttggcgtc tcaaattttt gaaaagccag atcaattgtg atacctcttt ctctttcgtc
     8941 tttaatgtta tccataaccc acgcgtactt gaatgtgtca ccttttccgg tcttttctga
     9001 ctcggctgca tgagatgcga tggtcctttc atctacaagg cccatatcca ttaagaaatg
     9061 acccattgtg gttgatttac cgttatcaat gtggcctgta acaatcaagt tcaggtgtgg
     9121 tttagttgcc ataggacttt cgttatgaga ggggtttatt agagttacga ttttttcaag
     9181 actttttgcc attttttgga tttttttgat cagataattg gttttagatt ttgcgcggcg
     9241 tcatctgatc tggaatacag agtttttgga gttttggggt aaaagacccg ataagtttgc
     9301 caaatcagtt gtcatttacg aatcctaatc aaagaaatta aatcaaagta gacaaccaga
     9361 tgattcatga aggttaccgt ttctgcgatc aaagctgatg tcgggggaat tggtggccac
     9421 acaagaccaa gcgatgggtt acttgatgcc gtaaggcgaa ccatcaaaag cgccggtagc
     9481 ctcctgcttg atcattacat tggatatgca ggcgacgacg tccatatagt gatgtcacac
     9541 acaaaaggcg ttgacaactc tgagatccac ggacttgctt ggaaggcatt tgaggagggc
     9601 gccagggttg ccaaggccga aggtctctac ggtgcaggcc aggacctctt aaaggattct
     9661 ttttcaggaa acgtaaaggg aatggggcca ggcgttgcag agctagagtt tgaggaaaga
     9721 ccaaacgagg catttacggt tctggctgca gacaaaacgg agccaggcgc ttttaattat
     9781 cctttttaca gattgtttgt cgatgcacta agtaacacgg gactaatagt aaacaaatca
     9841 cttgccgccg gagtcaagtt caacataatg gacgtagaaa aaggagaaat cgcagaactc
     9901 gtactgtggg aggacaagcc aatcttggag gccgccctga tgtatcctgg gagatatgta
     9961 gtctcatcag tgtacacaaa gtccggagag ccaattgtag catcctcaac tgacagactg
    10021 cacaacattg caggaacata tgtaggaaaa gatgatccca tcgcagtagt tcgaactcaa
    10081 aagaattttc cagcaacaga agagcttgga agcgtcttta acaacccaca ctatgttgcc
    10141 ggcaatactc gcggcagtca ccacatgcca ttaatgccag ttaagatcaa cactgctgca
    10201 acaattgact tttgcattcc actaattgaa gcgctagtgt tttccatgca tgatggtaaa
    10261 tttacagggc catttgatgg attctcaacg ccagactggg actatgtaag acagattgca
    10321 actcaaaaag ccctagcgat gcgaagtcag ggattcatac atcctgcaac ccttgtgcct
    10381 gcagagctag aatatgcaga gggatacaaa gcagtaattg atggtttgaa caaaaagatg
    10441 aaaccacttg agaagaaaaa tatccatggc agcaaaaagt acgaagatcc tgactaaatt
    10501 agtccaacat aaacagcaat agagttaagt gtaagaaatg aggatctaat atatgaggtt
    10561 caatctcatt gaatatctac gatctgttat ctttgggatt ggcatagtct cagtttctca
    10621 catcttttta acaatatttg ggcagacaat accaaatgca gtggttggtt ttttccgaga
    10681 ggttggcgga gttttgatac ttgccgtagt ctgtgcgttt gcatttacat ggtttcttag
    10741 agcaagaccg catcacaaac caaaaaaata ttcagtagta gtctttgacg tatttgggca
    10801 acaaacaaac attgaaggtt tgcgtaccga ttttagaaac cacgatgtgg cgtggagttt
    10861 tatgaagcag tacaaaaacg actatccaac gcacaacttt gcgatggttg ctgacaattc
    10921 agattctgcc aagaaaacaa ttttcagata catctaaaac acatcaaaag tcctaaatat
    10981 taaacaaagt caccccaagc aagttgccac tcgatgtcat tcaggaaaaa agcctaacag
    11041 aaataatcaa ctatgcctta gagtacccaa gcatggttct ttcctccaca aaaagcaacc
    11101 acatcgtacc cacatcgctt gccgattttg cgtatggcct ttatgttgga tacatttctg
    11161 gaatgttttt tgacaagttc cttgcagaaa acaagagatt cctcaatgac gatgagctag
    11221 tcgattttta ttcaaaactg gcaagtagga attctgagat caccctaaaa atcaaagctc
    11281 atctcaagac acagtagcat tacattcgac tcacttttat tttagaccaa gtaaaaagct
    11341 ccggtgtgaa gttttcctta atctcagaga cgtttgaaaa aatggaggcg acgacaaaac
    11401 gactggaact tacacagcat cttgttgact tgttcaagca gacaccacca gaaatcattg
    11461 caaaggtagt ctacctcatt caggggaaga taagaccaga ctttgaggga gtagaactag
    11521 gagttgcaga aaagcttgcc atcagggcat tatcaaaatc tgctggcatt ccaattaaga
    11581 aaatagccga gatttatgca gaggacgggg atttgggcca caccgcagca aaaatgatag
    11641 aacggaaaac acagactaca tttttgactc aggacatcac tgcagaacga gtatacgaaa
    11701 cattgtacaa gattgccaag cttgaagggg cccgctctca agacatgaag atgaaataca
    11761 tttccagtct actaaacgat gcaacgccgc aagaagctaa attcatcctc aaaatatcaa
    11821 tggggacttt gcgtcttgga attgcggaca atacagtaat ggatgccttg gcaattgcat
    11881 acactggcgc aaaagaaaac agagaaaaac ttgaagctgc gtacaatgtg tcatctgatc
    11941 ttggcagagt tgcagaggca attgcaaaca acaatcttgc tggattagaa aattttgaag
    12001 tgacgctgtt tagccccatt cggcctatgc ttgcagaacg cgtaaaaagc gaacgggagg
    12061 cagttgaaaa gcttggcaag gagtttgtag ctgagtacaa gctggacggc gagagggttc
    12121 aaattcacat caataacgaa aaagtggttt tgttttcaag aaggcttgaa aacattacaa
    12181 cccattatcc cgatattgtc gaaaatattc caaaggcatt aaaaaccacg gaagcaattt
    12241 tggaagccga ggccgtagcc atcaatgaca acactgggga atttttgcca tttcaggaat
    12301 taatgcatag aagaagaaaa tacgacattg caaaaaccgt agaacagtat ccaattactg
    12361 tcaacttgtt tgatttaatg tatctaaatg gacgagatct gctaaattcc acatatgagg
    12421 aaagaaggaa aaggcttgaa gagatcacga cagaaaacga ttttgtcaga ctggtaccaa
    12481 gaaccatcgt gaggtctgaa aatgagatcg aggattttct tgaaaatgca ataaatgcgg
    12541 gatgcgaagg gctaatgctc aaagttctcg atgcacctta cagggctggg gcacgcggaa
    12601 gtaattggct gaagctaaag cgagaatata gaaacgagct tggagacagc cttgacctag
    12661 ttgtaattgg ggcatttttt ggcaggggaa ggcgcaccgg caaatacggt acgctcttgc
    12721 ttgccacata caatcaagaa gatgacacct ttgatagcat ctgcaaagtt gggaccggtt
    12781 ttacagatga gaaccttgat cagttttatc agattctctc tgacaaagtt actctgaaga
    12841 aaaatcctag aattgacagt ggaatggaag ctgatgtgtg gtttgagccg gaattggtaa
    12901 ttgagatagt tgcttccgaa ataacactaa gcccaattca cagaactgca aaggacgtga
    12961 tccgaaaggg aagcggtctt gctctgaggt ttccaaaatt cacaggtaag ataagaactg
    13021 aaaaggcgcc tgaggatgca tcaactgacc aagaagtaat cacactctat agggaccaga
    13081 aaaaagtaac acagggtgtt tccgaaagct aagcctgcaa gattaattaa taccatgcaa
    13141 aattatacaa aaggatgtat agcggagagc tagaggttca agcaaaacga aaagcaattg
    13201 cagttttaca agacgaaata aacagaatcc tcaatgcggc aagagagctt gcaacattac
    13261 ctgctttggt tatgaaaaaa gacaaggttg gaatcaaggc agtaaatgag caaatctcaa
    13321 gcatcgaaga agaagttgaa aaccttagaa gaaaaatcac tcgtgaggtt gccgatgtag
    13381 gcggtctgat catgaacaga gagaatctgt taaacactgc gtacacaatg gatgagattg
    13441 caggatatat cacgggaatt gcctttaagc tttcacaaat caaaccaact actctaaaga
    13501 atgcaaagat ggatgtcgat atttctgcat taatagattt ggtagtagac caagtctaca
    13561 agcttaacga aatagtccgt ggcctcaaca caaacactgc aaacgcaatc gagcttgccc
    13621 aagaaaccca aaaaatcgaa cgtgaaatcg atataaaata tcgcacgaca gtagtcaagg
    13681 cattagacga aattagcaat acaaaagaac tgatgctagt caaagacgtc atacagggaa
    13741 ttgaagagat ggcagacaag tgccagcaag tttctgactc attcatactc cttgcactaa
    13801 gcctttaacc caatctttca tatttttaac acaaagcaaa taatttttag tgatagttta
    13861 atgacaatat catgcaaggc gagcttgtag aaaacagaat catagtttgg agcacaaagg
    13921 atgcaaggag tctttttact gattcctact atggcaagcc aattggaatc ccaaaaccca
    13981 aaccagagga gatcaatgtt ccgttgattc tggatttaat tgaggggcat tatctgcaag
    14041 aaaaaggaga aatcacaatt ttcaaatcaa aaaagaaaat aacgagacag caattactcc
    14101 aaatctgcag aaacgaatat cataactttg ataaaaaata cctagtttac aaagacttta
    14161 gagagaaaaa atacatcgta aaccccggca taaaatttgg atgtgatttt gcagtttatc
    14221 agaggggacc gggagtagat catgcaccat atttggttca agtatacaac aaaaacgaca
    14281 ctctctcatc aactggcgtt gtacttgcag gaaggctggc aacaagtgtt aaaaaaaact
    14341 ttatccttgc aataccgcat ggaccgaagg taaactattt ggcacttgat tggtggaaag
    14401 cctagatttt ctttacgtgt tctgcaatct tgtcataaat tacatacaag tcttttgtta
    14461 ttttaaagtc gtaagcgttt ttccatttgt tgtagattct gatttccttg actgcaggat
    14521 caacaaaaat tgttgcgtct ttgaggggtt gttttgctat gagttcattt agctcagtgt
    14581 ccttgttcag ctcctcggca agaagaccgc cattccaact aacggacttt actttttttg
    14641 cgccaaaatg gccttcagtt tttaggatgg tctttgcaac gtatttttcc ggacgagcgg
    14701 catccatcct cacaataaaa tgtgcctgaa atttgtcttg cgccccccag tgagagattg
    14761 ttggatcttg catttttttt accccttttg aattatctga attacgtcaa tgtttgaatt
    14821 ttttagctca acacagcccc tgtttgtgat catccttgtg gcttgagaga aatttctccc
    14881 atagtagtcc ttggcatttt caactggatc ggttccaagc tcctttggtg tggcatctaa
    14941 gccgatttct tttagcatgt gggatacttt ttcaggccat gtttcataaa caaaagtgtc
    15001 agactctgtg tcatgcatat tttttgttgg ttttacaccc acaaataaag ttttgatatg
    15061 cgattgatcc aattaaatat tccaaaacta atgccaattc catgttaaag tttcagaaca
    15121 agatcgtctt agttacagga agcggcaccg gaattggtca aacagttgcc aaactgttcg
    15181 cagaaaacgg cgcaagcgta atcattttgg gaagacgaaa agagccactt gaagaaacaa
    15241 agaagatttt agacgatatt atcaataatg ccaaaagtaa cgccaaagtc tggctgtttt
    15301 ccggagtcga cgtcagtgac gaagaaggcg tatcacaaat gttcgattcc ctaaagcaat
    15361 ca
//
LOCUS       DASWKH010000128         8487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3288160, whole genome shotgun sequence.
ACCESSION   DASWKH010000128 DASWKH010000000
VERSION     DASWKH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288160"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGA92_09325"
     rRNA            <1..412
                     /locus_tag="VGA92_09325"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            602..982
                     /locus_tag="VGA92_09330"
     CDS             602..982
                     /locus_tag="VGA92_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX9846642.1"
                     /translation="MAESMELQFFESKCKKLTQQHEIRFAGIIDENGRLIAGGFKEGL
                     EPLEENESRLNAFMEFASTVSLRKEFDKTLGPINYLAARRDKLVLISFPFPVSKFVLL
                     ISAEPTVDIESVAKKVVDEFTNVS"
     gene            complement(977..1492)
                     /locus_tag="VGA92_09335"
     CDS             complement(977..1492)
                     /locus_tag="VGA92_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846643.1"
                     /translation="MSGFLICVCIGFASLQSFASTNYLTENDIFYCEENYEQYKIIGE
                     YDFLVRERHTLEARVCVHLYNDPLWADTSKYRTAKLLERGNYYVNVEIEKSKENAESG
                     TIPQPEKPISDMQKAINQITALEKKVSDLEKKLAQKDAVIFEQIKVIMDLANKIKTVI
                     FSHVPFLNLSL"
     gene            1587..1817
                     /locus_tag="VGA92_09340"
     CDS             1587..1817
                     /locus_tag="VGA92_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9846644.1"
                     /translation="MNEAYVLLNVDYKQQKNIIEQAKKMPAVKSIKTVYGIYDILVVL
                     ESENMQEIKNTIDIHLHNIDGINNLTSLISVT"
     gene            1818..2792
                     /locus_tag="VGA92_09345"
     CDS             1818..2792
                     /locus_tag="VGA92_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9846645.1"
                     /translation="MVQEYEVVIVGAGPAGLSAGIFVARQKTSCLVISKDLGGQLNLI
                     PKLENYPGTMMSSGPLLAKTLENQYLVFGGDLTYDTVERIDEVEDGIKVKTARSEYLA
                     KAIVLAPGKVPNNIGLENESQFANKGVHYCTKCDAPFYQGRTTATVGVGGYLLESGIL
                     LSRMASKVYLIYRGGGLGGDKEMIEAIKKKENVELVPNTSIKSISGSNTLQQITVVDS
                     TGAEKTINVDGLFIEMGSKINLDFVKHLVKINQKGEIEVTDGGKTSHPAIFAAGDATC
                     IPYKQIVSACGDGAAAGLSAFNHVEKLKGRPGVRADWKKTIGDTVFHY"
     gene            2859..3440
                     /gene="dps"
                     /locus_tag="VGA92_09350"
     CDS             2859..3440
                     /gene="dps"
                     /locus_tag="VGA92_09350"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HEX9846646.1"
                     /translation="MDKQTEPNVVGVQVLKQNGVDTDELIRLLVYNASVEFTAYYYFT
                     NLRMHCTGLDGEGVKGVVEDARLEDLSHFESCLSRIYELGGSIPNDAMEFIKMSGCEF
                     LQLPPNKTDLRAIMEKCLKAEQGAIVNWNKVCQMTIGKDPATYDVAKDILREEIEHEA
                     WFLELLYGRPSAHMRRKFPGERPHTHKHSRALG"
     gene            3553..5055
                     /locus_tag="VGA92_09355"
     CDS             3553..5055
                     /locus_tag="VGA92_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9846647.1"
                     /translation="MILGIIFPPVFGHGVGYEILPPQMLGDRKVAMEVGSTVDNATNR
                     KQIVFSMFDTNTGLSVRDVTYHIKTIKNNQILFEGSYQTTNGVLTFDLIPDASDKVIV
                     QEKKDSDIFGFLIGAKKSLVEAKGKIFERGGLYKFSIRVVSAENYSDKTQKPVQFESG
                     LSFAEPTIYNVNDKQFGKQELKVVSYYDVFDGVIFDSKTRSVFFSMPFEWTINNINQT
                     SVIHQEVFIPKTFTALQVSEYEIFANDSILPNNAITIDDFVNDYRVIHILLYQTELMN
                     LYEKQEKEQSKIIFSLTPKSDDVLLVDVTENVQYKITMTMMPKQIVSGQETTLLFKIY
                     DVFLQGKAVSVNYDLLVKSGDTVLYQTSGTSSDSKEKWNEAKFDIPKDVANIISVRFE
                     NLGGNRLARAEIPLMVSTIDGQEVPSWIKNNAGWWCQKLISDDEFLRGVEYLIDKNII
                     DVGTQAQSTSQKGIPEWVRSNSCWWSNNSISDNEFVSGIAYLVKVGIIKA"
     gene            5166..5957
                     /locus_tag="VGA92_09360"
     CDS             5166..5957
                     /locus_tag="VGA92_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9846648.1"
                     /translation="MQEEFISVEGNKIRYLKAGNAKKNLILVHGLGASAERWEYVIPK
                     FSKHYTVFVPDLIGFGKSDKPNADYTAEFFTNFLSSLLNALGLEKATMMGSSLGGQIT
                     VEYALNNQKSVDKLVLVSPSGAMKQSTPALDAYVMAALYPDHEIAKNAFTMMTGSNKK
                     VSPHIIENFVQRMKLPNAKFAFMSTLLGLKNAPEITTRLEVITAPTLVVWGELDPVIP
                     VTYAEYFVKNIRDCRFYQMQNCGHTPYVEDPENFSKIVLDFLLQK"
     gene            5989..6399
                     /locus_tag="VGA92_09365"
     CDS             5989..6399
                     /locus_tag="VGA92_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9846649.1"
                     /translation="MAGNTNQTDYVAMFQDWMQKGGKAQAEFMKAFCSFMENNQRFDP
                     LQTLKEVTSKAAEAQAGLVSNVASMQKSAMEQAFNVANMMQSFTGWGAFKTTVGSNGR
                     ISIPEAERDALRLNEGDLVQVMVLPLEKKKPKKE"
     gene            complement(6402..7460)
                     /gene="phaC"
                     /locus_tag="VGA92_09370"
     CDS             complement(6402..7460)
                     /gene="phaC"
                     /locus_tag="VGA92_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HEX9846650.1"
                     /translation="MQPETIDPKLVEEFLSFTKNVTDAHKLVPAPDEIILESTPFDVA
                     YEEDRMRLLHFKPLTEKQVKTPLIISYAIINRFHILDIQPKKSWVRKILESGIDVYLI
                     DWGNPSSIDKYLDFDDYVNTYMDNCVDFVRNESQTENVSLQGYCTGGTIATIYSALHP
                     QKIKNLITTAPVIDGWKDTTVVCNMAKHIDVDKIVETIGNMPPEFMYYCFSILKPFEQ
                     GLEKYYKFFKNIHDKEFVDSFLRVEKWLSETPPIPGELFKQWIKDVYQENLLIQNKMY
                     VGGKHVDLKKITMPLFIQIAVGDHLVSPECSIPLHYAVGSQDKTLRIYPTGHVGMIAS
                     KFSQQQVLPELCQWLKER"
     gene            complement(7450..8013)
                     /locus_tag="VGA92_09375"
     CDS             complement(7450..8013)
                     /locus_tag="VGA92_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HEX9846651.1"
                     /translation="MSQDTKGPAEYYKHISLFWTDLLHLMSGKPMALTSVGPMRNWTN
                     DMKKVQTEVIDSYEDLMEFNQRLVGYYKQLSDAWTESQKKVNAKIPSIPNDSEQLEAY
                     KRIWIDIFENDFTELFNSEKFGQNYGKLVSAELELTKHWENILNVLLNSMRLPTRKEI
                     DEVYKELHELRKRVKKLEKKEVKSDAA"
     gene            8088..8447
                     /locus_tag="VGA92_09380"
     CDS             8088..8447
                     /locus_tag="VGA92_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX9846652.1"
                     /translation="MDKETLCKKISELDPMIRFVGLINDKGHLVAGGMAEGKKPLEDT
                     KKDEMLYMELALRVRMRQEFDVELGPVKFALSYRDRVIVLSFPVGREILLVSAEKDLN
                     FGKFPFSVLKLIEEYSK"
BASE COUNT         2633 a         1630 c         1836 g         2388 t
ORIGIN      
        1 gcggtttggt acctcgatgt cggcttttcc tatcctggtc ctgcagcagg ggccaagggt
       61 gaggctgctc gcctattaaa agggaacatg agctgggttt agaccgtcgt gagacaggtc
      121 ggtctctgcc tgatggaagc gtggctatct gaggggaagt tgctccgagt acgagaggaa
      181 cagagcagcg tggccactgg tttaccggtt gtctgacaag gcaggccggg cagctaagcc
      241 atttgggcta agtgctgaaa gcatctaagc acgaaaccct tcccgagaca agatagccct
      301 tctttcgatg gaggtcagca gtagaagact gcgttgatag gatggaggta tagatcacaa
      361 gcttcggcga gtggtgagcc tgccattact aacagaccaa cgcactggtt agccacatta
      421 catagagact atgcgcgatg atttttaatt ttaaatcaac cctagtggac actaggtttt
      481 caaaaattac acgactaatc aaaataactt ttcagaaaat gttgccacaa aatacgatgg
      541 tttagggcaa attcatttct aaaatgtcaa atcttttatg tcggactggc ttagattctg
      601 tatggcagaa tcaatggagc tgcagttttt tgaatcaaaa tgcaagaaac tgacgcaaca
      661 acacgaaatc aggtttgccg gaattattga tgaaaatggg cgtcttattg ccgggggatt
      721 caaagaaggc ctagagccgc tagaagaaaa cgaaagcaga cttaatgcat tcatggaatt
      781 tgcctcaacg gtatcgctaa gaaaggagtt tgataagacc ttgggaccaa tcaattatct
      841 ggctgcaagg cgcgacaagc tggttctcat aagctttcca ttcccggtaa gcaagtttgt
      901 tttgttaatt tctgccgagc cgacagtgga catagaaagt gtcgcaaaaa aagtagtcga
      961 cgaatttacc aacgtctcat aggctcaagt tcagaaaagg aacatgagag aaaattactg
     1021 tttttatctt gtttgcaaga tccatgatta ctttgatctg ctcaaaaatc accgcgtcct
     1081 tttgagctaa ttttttttca agatcggaga cttttttctc aagtgcagtg atttggttta
     1141 tggccttttg catatctgaa atcggctttt ctggctgagg tattgtacct gattctgcgt
     1201 tttccttact tttttcgatt tcgacattta cgtaatagtt tcctcgctca aggagttttg
     1261 cggttctata cttgctagta tcagcccaaa gcgggtcgtt gtacagatga acacaaacac
     1321 gagcctcaag tgtgtgtctt tcgcgcacta aaaaatcata ttcaccaata atcttgtatt
     1381 gctcgtaatt ttcttcacag taaaaaatat cgttttctgt caagtaattt gttgatgcaa
     1441 atgactgcaa gctggcaaat ccaatgcaca cacaaatgag aaaacccgac aactttatgg
     1501 cgaaattttg ttttggcagt taaagaaatt tactactcgg tgatatgaaa tgaaactctt
     1561 ttaaagcaac catatgagga tatgatatga atgaagcata cgttttgtta aatgttgatt
     1621 ataaacagca aaaaaacata attgagcaag caaaaaaaat gcccgcagtc aagtcaataa
     1681 aaacagttta cggaatttat gatattttgg tggtattaga gtcggaaaac atgcaagaaa
     1741 taaagaacac aatagacatt catctccaca acatagatgg tatcaacaat ttaacatcgc
     1801 ttatctcagt gacataattg gtccaggaat acgaagtagt tatcgttggc gcaggacctg
     1861 caggactatc tgccggcata tttgtggcaa ggcaaaaaac atcatgtctt gttatttcaa
     1921 aagaccttgg agggcagctg aatcttatcc caaaactaga aaattatccg ggaacgatga
     1981 tgtcaagtgg gccgcttttg gccaaaacct tggaaaacca atacctcgtt tttgggggag
     2041 atctgacata tgatactgtg gaaagaatcg atgaggttga agatggcatc aaagtcaaaa
     2101 ctgcacgctc tgaatatctg gcaaaagcaa tagttcttgc cccaggcaag gtcccaaata
     2161 atattgggct tgaaaatgaa agccagtttg caaacaaggg cgttcattat tgtacaaagt
     2221 gtgacgcgcc attctatcaa ggcaggacca ctgcgacagt cggtgttggt gggtatcttc
     2281 tagagtcagg aatccttctc tcgcgtatgg catcaaaggt ttacctaatc tacaggggcg
     2341 gtggactggg tggagacaag gaaatgattg aggcaattaa gaaaaaagaa aatgttgaac
     2401 ttgtcccaaa cacttcgatc aaatccatct caggaagcaa caccttgcag caaattacag
     2461 ttgttgacag tacaggtgct gaaaaaacga tcaacgtgga cggcttgttc atagagatgg
     2521 gctcaaaaat taatctggac tttgtaaaac atcttgttaa gatcaatcaa aaaggggaaa
     2581 ttgaggtaac cgatgggggt aaaacctcgc acccggcgat atttgcagca ggcgatgcta
     2641 catgcattcc atacaagcag attgtttctg catgtgggga tggtgctgca gcagggcttt
     2701 ctgcattcaa ccatgttgaa aaactaaagg gaaggccggg ggttcgcgcc gactggaaaa
     2761 agacgattgg cgatacggta tttcattatt gattttgcaa ctgaaaacca gatttataga
     2821 accaataatt tttatctaca tgttttatgt ttattcgtat ggataagcaa actgaaccaa
     2881 acgtggttgg cgtccaagtc ctaaaacaaa atggtgtgga tactgacgaa ctcatcagac
     2941 ttcttgttta caacgcctct gtagagttta ctgcatacta ttactttaca aatctgagga
     3001 tgcactgcac tggccttgat ggtgaagggg taaaaggcgt agtcgaagac gcaaggcttg
     3061 aggatctaag ccactttgag tcgtgccttt cgcgaatcta tgagcttggc gggagcatac
     3121 caaacgacgc aatggaattc atcaaaatgt cgggctgcga gttcttgcag cttcctccaa
     3181 acaagacgga ccttagggca atcatggaaa aatgcctcaa agccgaacag ggcgctatcg
     3241 tcaactggaa taaggtctgc caaatgacca tcggaaagga tcctgccaca tatgatgttg
     3301 caaaagacat cctaagagaa gaaattgaac acgaggcatg gttcttagag ttactttacg
     3361 gacgaccatc agctcacatg agacgaaaat tcccaggaga gaggcctcac acccacaagc
     3421 actcaagagc attgggttaa catctttttt tcttttatga ttggtgttga tgacaaaatc
     3481 tgttttacat ggaaagcctt ctgtaatgct gatatgaagt caaatcaaat tatcatttta
     3541 ttatcttgca ttgtgatttt aggaattatt tttcctccgg tatttgggca tggtgttggc
     3601 tatgagatac ttccaccaca aatgcttggc gacagaaaag ttgcaatgga ggtaggctct
     3661 acagttgaca atgcaacaaa tcgcaaacaa attgtttttt caatgtttga tacaaacaca
     3721 ggacttagtg ttagggatgt cacataccac atcaagacaa taaaaaacaa tcagatactc
     3781 tttgaaggaa gctatcagac aacaaacggg gttcttacat ttgatctaat tccagatgca
     3841 agcgataaag tcatagtcca agaaaaaaag gactctgaca tttttggctt tctaattggt
     3901 gccaaaaaaa gcctagttga ggcaaaaggc aaaatctttg agcgaggagg tttgtacaaa
     3961 ttttcaataa gggtagtttc cgctgagaat tattcagaca aaacgcaaaa accagtccaa
     4021 tttgaatcag gactctcctt tgcagagccg acgatttaca atgtaaatga caaacagttt
     4081 ggcaagcagg agctcaaagt ggtcagctac tatgatgtgt ttgatggggt aatctttgac
     4141 tcaaaaacaa ggtccgtttt cttttcaatg ccttttgagt ggacaattaa taacataaat
     4201 caaacgtcgg taatccacca agaagtcttt attccaaaaa catttacagc attacaggta
     4261 tctgaatacg aaattttcgc aaacgattca attctgccca acaatgccat aacaattgat
     4321 gatttcgtaa atgactatcg tgtaattcac atcttgctat accaaacgga actgatgaat
     4381 ctttatgaaa agcaagaaaa agaacaaagc aaaattatct tttcattaac gccaaaatcg
     4441 gatgatgttt tgttggtaga tgtaacagaa aatgtgcaat acaaaattac catgactatg
     4501 atgccaaaac agatcgtatc ggggcaagaa accacactgt tgtttaaaat ttatgatgtg
     4561 tttttacaag gcaaggcagt atctgttaat tacgatctct tggtaaaatc aggtgataca
     4621 gttctttacc aaactagcgg caccagctcc gactctaaag aaaaatggaa cgaagccaaa
     4681 ttcgatattc caaaagatgt tgcaaacata atatcagtaa gatttgaaaa ccttggtgga
     4741 aatcgacttg caagagcaga aattccactc atggtgtcga caattgatgg gcaagaagtg
     4801 ccatcttgga ttaaaaacaa tgccggatgg tggtgccaaa aactaatttc agatgatgag
     4861 tttctcagag gtgtggagta tcttattgac aaaaacatca ttgatgttgg aacccaagcc
     4921 caaagtacat cgcaaaaggg aattccagag tgggtaagga gcaattcttg ttggtggtca
     4981 aataactcga tctcagataa cgaattcgta agtggaattg cgtatttagt taaagttgga
     5041 ataataaagg cctaaattcg gccattatct gccataacta gtctaacatt atagatccgt
     5101 tcgtccaaga tgatcaacct aaacctaaaa cttatctatg ttaacattag attgacgata
     5161 actcgatgca agaggaattc atttcagttg aaggaaacaa gatccgttat ctaaaagcag
     5221 gaaatgcgaa aaaaaacctg attttggttc acgggcttgg ggcatctgca gaaagatggg
     5281 agtatgttat tcccaaattt agtaaacact atacagtttt tgtcccagat ctgatcggat
     5341 ttggtaaaag cgacaagccg aacgcggatt acacagcgga gttttttaca aactttcttt
     5401 cttcgctttt gaatgctttg gggcttgaaa aagccacaat gatggggtca tcccttggcg
     5461 gacaaattac agttgaatat gcccttaaca atcaaaaatc tgtagataag ctagttttag
     5521 tatcgccttc tggggcaatg aagcaatcta ccccagcact tgatgcatat gtgatggctg
     5581 cactttatcc tgatcatgag attgcaaaaa atgcgtttac aatgatgact ggtagtaaca
     5641 aaaaggtaag cccacacatc atagaaaact ttgtgcaaag aatgaagcta ccaaacgcaa
     5701 aatttgcatt catgtccact ctgcttggac tcaaaaatgc accagaaata acgacaagac
     5761 ttgaagttat cactgctcca acgttggttg tgtgggggga gcttgaccct gtcattccag
     5821 taacgtatgc cgaatatttt gtaaaaaata tccgggattg cagattttat caaatgcaaa
     5881 actgcggaca caccccgtat gttgaagacc cagaaaactt tagcaagatt gtacttgact
     5941 ttctactcca aaaataactt taaataacaa ctttaccaca aaatgccaat ggctggtaat
     6001 acaaaccaaa ctgactatgt ggcaatgttc caagactgga tgcaaaaagg aggcaaggct
     6061 caggccgaat ttatgaaagc attttgttct tttatggaaa ataaccaaag attcgatcca
     6121 ctgcaaacgc tcaaagaggt gacatcaaaa gcagccgagg cccaagctgg ccttgtaagt
     6181 aacgtggctt ctatgcaaaa aagtgccatg gagcaggcat tcaacgtggc aaacatgatg
     6241 caaagcttca cgggctgggg tgcgtttaag acaacggtgg gaagcaacgg acgaatatct
     6301 atcccagaag cagagcgtga cgcattaagg ctaaacgaag gcgatctggt tcaggttatg
     6361 gttcttccac tagaaaaaaa gaagccaaaa aaagaataac tctacctttc ttttagccac
     6421 tggcaaagct caggcaaaac ctgttgttgt gaaaatttgc ttgcaatcat tcctacatgc
     6481 cctgtaggat aaattctcag agttttgtcc tgacttccta ctgcataatg aagcggtatg
     6541 ctgcattcgg gggaaactag gtggtcgcca acagcaattt gaatgaataa tggcattgta
     6601 atttttttga ggtctacgtg tttgccgcct acatacatct tgttttgaat cagcaggttt
     6661 tcctgataca catcctttat ccactgcttg aatagctcgc ctggaattgg gggcgtctca
     6721 cttagccatt tttccactct cagaaagctg tcgacaaatt ctttgtcgtg tatgtttttg
     6781 aaaaacttgt agtatttttc cagcccctgc tcgaatggct ttagaattga aaagcagtaa
     6841 tacatgaact cgggaggcat gttgcctatt gtctcaacaa tcttatctac atcgatatgt
     6901 tttgccatat tgcaaacgac cgtggtgtcc ttccatccgt caattactgg agctgtggtt
     6961 atgagatttt tgattttttg ggggtggagt gcggaataaa tagtagcaat tgtgcctcca
     7021 gtgcagtatc cttgcaagga aacattctct gtctgagatt cgtttcttac aaaatcaaca
     7081 caattatcca tgtatgtatt tacataatcg tcaaaatcca aatacttgtc gatgcttgat
     7141 gggttgcccc aatcaatcaa gtatacatca attccacttt ctaagatctt tcgcacccaa
     7201 cttttcttgg gctgtatgtc aaggatgtgg aatctgttta ttatggcgta gcttatgatg
     7261 aggggagtct ttacctgttt ttctgtgagt ggcttaaaat gcaataaacg cattcgatcc
     7321 tcctcatatg ctacatcaaa aggcgttgac tctaatatga tctcgtctgg cgctggcaca
     7381 agtttgtgtg cgtcagtaac gttttttgta aaactaagaa actcttcaac taactttggg
     7441 tcaatagttt caggctgcat ctgattttac ctcctttttt tctagcttct ttacacgttt
     7501 tctaagctcg tgcagctcct tgtagacttc gtctatttcc tttcgtgtgg gcaatcgcat
     7561 tgagtttaag agcacattga gaatgttttc ccaatgcttt gtgagctcca gttctgctga
     7621 tactagtttg ccgtaatttt ggccaaattt ttcagaatta aataactcag taaagtcatt
     7681 ctcaaaaata tcaatccaaa ttctcttgta ggcctcaagc tgctctgaat cgtttggtat
     7741 gcttgggatc tttgcgttta cctttttttg agactcggtc catgcatctg ataattgctt
     7801 gtagtatcct actaatcttt ggttgaactc cattaggtcc tcgtaggaat cgatgacttc
     7861 ggtctgaacc tttttcatgt cgttagtcca gttccgcatg gggccaacag aggtgagtgc
     7921 catgggcttg ccagacatta ggtgaagcag gtctgtccaa aacagagaaa tgtgcttgta
     7981 atattcagct ggtcctttgg tatcctgcga catgctaatt aaatacaaaa gatcgcaata
     8041 aatagatcac tcctaaaagg acaggtacgg gtaattagtg aaaaaacatg gataaagaaa
     8101 cactttgtaa aaaaataagc gaacttgacc caatgattcg gtttgtgggt ctgataaatg
     8161 acaaaggtca cctagtcgca ggagggatgg cagaaggaaa aaagccactt gaggacacaa
     8221 aaaaagacga aatgctctac atggagcttg ccttgagggt cagaatgcga caagaattcg
     8281 acgtcgaact agggccagtc aaattcgcac tgtcttatcg tgacagagtt attgtgctaa
     8341 gctttccagt aggaagagaa atactgctag tatcagctga aaaggatctt aattttggca
     8401 agtttccatt ctcggtatta aaactaatag aagaatattc taaatagaaa tatccgctta
     8461 gtttatgatg tgattggtag tgtgttg
//
LOCUS       DASWKH010000129         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3293891, whole genome shotgun sequence.
ACCESSION   DASWKH010000129 DASWKH010000000
VERSION     DASWKH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3293891"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VGA92_09385"
     CDS             complement(<1..505)
                     /locus_tag="VGA92_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846653.1"
                     /translation="MADVLAVFAGLSYLGVILLLFLVNMAPVLMPPTWLILASFYVLD
                     DSLNPLSLALAGATGATAGRFLLLYCSVYFRRFMNQERKSSLEKITNYLRSKRFGFFL
                     VTFLFAATPLPSNMLFIGYGLMKAKTIQIYIGFWLGRVLSYYVMISISKIVLTPFAQL
                     FEDRLLGI"
     gene            824..1249
                     /locus_tag="VGA92_09390"
     CDS             824..1249
                     /locus_tag="VGA92_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846654.1"
                     /translation="MRRRGISEAVSAVMLLAVIATVSYFALSGASKKTIDAERTVVDV
                     IELKGAQVQELLSVISKNASPGSITLELLNYGKKDITIERVFVDGSPFAFTVSDSDGN
                     VYSNNTITPKKITELYVGAAGQSIQIVTSSKNIINLPLN"
     gene            1280..>1553
                     /locus_tag="VGA92_09395"
     CDS             1280..>1553
                     /locus_tag="VGA92_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846655.1"
                     /translation="MKKITVLLIVILFCSYITPAYAESSPEILYSGLVSGGGPEIVTE
                     THYWGFCKIWVETGISKSYSNGFVRNNPDKSFYPGDGFGYIFVYGWA"
BASE COUNT          472 a          334 c          320 g          427 t
ORIGIN      
        1 taattcccaa aagcctgtct tcaaataatt gggcaaatgg agtcaggaca atcttgctaa
       61 tcgatatcat cacataataa gacagtaccc ggccaagcca aaacccgatg taaatttgga
      121 tggtctttgc cttcatcagt ccatacccga taaagagcat gttgctaggc aacggtgttg
      181 ccgcaaacaa aaacgtcacc aagaaaaacc caaatctttt tgatctgaga tagtttgtga
      241 tcttctcaag gcttgatttt ctttcctggt tcatgaacct tcgaaaatac acactacagt
      301 aaagtaaaag gaaccgccct gccgtagctc cagtagctcc agctagcgcc aaagaaagcg
      361 ggttaagcga gtcgtccagc acgtaaaatg acgccaaaat caaccaggtg ggcggcatta
      421 gaaccggagc catatttacc aaaaaaagca gcaaaatgac tccaagatat gaaagtcctg
      481 caaaaaccgc taaaacgtct gccacgacaa tacctgtctt gcgcctttac taaattggtt
      541 ttgagccctt caaaattagc ccgttgttac agaaaaaatg aaatttttgg gcgttttgat
      601 cggatttgac aaaaaaatgg acaaaacatc caacatcttt aaattttaag cagcctatat
      661 agtactatga gcgccaaggt gtggaagtgt tttagatgca atcttgtttt ccgtgaggaa
      721 tccttggctg atgtgcacac caatttgtca agacatcatg taaccagcat tgacgttttg
      781 gaagcataat acgccttaaa acaaacaaaa ttcttttcag ttattgcgcc gacgtggaat
      841 ttctgaggcc gtaagtgccg ttatgctttt ggcagtaatt gccactgtgt catattttgc
      901 cctaagcggc gcctcaaaaa aaacaatcga tgctgagcgg accgttgtag atgttataga
      961 gctaaagggc gcccaggtcc aggagctgct ctcggtaatt tccaaaaacg catcacctgg
     1021 cagcataacc ttggagcttc taaactatgg gaaaaaagac atcacaattg agagagtgtt
     1081 tgtcgacggc agtccttttg catttacagt ttccgattct gatgggaatg tttattcaaa
     1141 caatacaatt actcccaaga aaatcacgga gctttatgtg ggtgctgctg gccagtcaat
     1201 ccagatagtt acatcttcta aaaacataat caaccttccc ttgaactgat gctttttagc
     1261 cagcattcat ttaattttgt tgaaaaaaat cacagttctt ctaattgtaa ttctgttttg
     1321 cagttatatc acaccggcat atgccgaatc aagccctgaa atcttgtaca gcgggcttgt
     1381 ttctggcggt ggcccagaaa tagtcacaga gacccactac tggggatttt gcaaaatatg
     1441 ggttgagact ggtatctcaa aatcatattc taatggtttt gttagaaaca atccagacaa
     1501 gtcgttttat ccaggcgacg ggtttggcta catctttgtg tacggttggg cag
//
LOCUS       DASWKH010000130         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_325757, whole genome shotgun sequence.
ACCESSION   DASWKH010000130 DASWKH010000000
VERSION     DASWKH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325757"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..866
                     /locus_tag="VGA92_09400"
     CDS             246..866
                     /locus_tag="VGA92_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9846656.1"
                     /translation="MRNLKELQNEVQKRIQNDPAHDFEHIMRVCRNAKKIAKHENADV
                     GLVMAAALLHDIVSYKKSDKRSKNASLQSAQAAKSILKKYDYATPEIKIITDAIRDHS
                     FSQNKVPKTLVGKVLQDADRLDALGAIGIARAFSVGGAENRPIYNTADPFCKKRTPND
                     KSWTTDHFFRKLLLLEKKMNTKSAKKEAQRRTRLMIKFLANLKREI"
     gene            complement(863..1174)
                     /locus_tag="VGA92_09405"
     CDS             complement(863..1174)
                     /locus_tag="VGA92_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846657.1"
                     /translation="MKEEERFEIERAYDLLPHVVGASWACVWFRLNQIKKPTAEEFRQ
                     KTIEYLAVLERLYESYPGTEQFSEINSYMKNRHTEEIEKILEGKNPEIEKRYARYIDY
                     G"
     gene            complement(1171..2157)
                     /gene="hemB"
                     /locus_tag="VGA92_09410"
     CDS             complement(1171..2157)
                     /gene="hemB"
                     /locus_tag="VGA92_09410"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9846658.1"
                     /translation="MLGSFPSKRLRRLRKNEKMRNLVQEITLSTNDLICPVFVQEGLK
                     SRTQVQSMEAIERLPLSELTKEVESIKNLGIPAIMLFGIPEHKDEQGTSAFAENGIVQ
                     EAISQVRKNFGDSMVIMADVCLCQYTTSGHCGIAKGNVIDNDSSIETLAKIAQSQAAA
                     GVDIVSPSAMMDGQVKAIRHRLDENGFTEVGIMSHSAKHRSAFYTPFRDAADCAPHFG
                     DRKTYQVPFTNPREAMREIELDVNEGVDIVMVKPALAYLDLIAEAKRRFNIPISAYSV
                     SGEYALVKGAAKQGWINETDVMTEILYSIKRAGADMIVTYFAKMMAQQLLQK"
     gene            complement(2205..>2955)
                     /locus_tag="VGA92_09415"
     CDS             complement(2205..>2955)
                     /locus_tag="VGA92_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9846659.1"
                     /translation="LAEENIDELKSKKILLIGTGEAATLVAKSLIKRDYGFFVTSRTM
                     ERSKAFAETIGGNPISFEDILTSFSNYDVVFVATIAPYFLVTFDRISEAMKDKKNGMM
                     ILDLSNPRTVDEKVATIHGIKMMNLDQIAEMVDKNIRYRSSQRSNAEKIISEEMQVLQ
                     AHMKRLEVEPVVKEIFKDIDVRRSKELQKALQMLGEKDAEKIKIIDELTKAVVEGIIS
                     EPMNNLRRASEQSDDELLKAAAKLFDYKKKE"
BASE COUNT          754 a          677 c          600 g          924 t
ORIGIN      
        1 tgctgtcttg gcaatctcgg cccgttcttc aactagggag tttatcagat tcatcatctc
       61 ttttgcgtcc gaggcctttg ggtgcctgaa aacgacgatt tttccgccaa ttacctgttc
      121 tttgagtatt ctgccagccg attttgtgcg tatttttgcc atttttacca atttttcctg
      181 ttattgtgaa attggagtaa tatggcacaa aaagctattt gtacgaacaa aatttttgaa
      241 aaactgtgag aaacctcaaa gaattgcaaa acgaggtgca aaaaagaatc caaaacgatc
      301 ctgcccacga ctttgagcat ataatgcgcg tctgcagaaa tgcaaaaaag attgcaaaac
      361 acgaaaatgc agatgtaggg cttgttatgg cagctgcgtt attgcacgat attgtatcgt
      421 ataaaaaatc agacaagaga tcaaagaatg catcgctgca aagcgcgcaa gctgcaaaaa
      481 gcatactaaa aaaatacgac tacgccacac ctgaaataaa aataattact gatgcgattc
      541 gtgatcacag cttttcgcaa aacaaagtgc caaagactct ggtgggaaag gtactacaag
      601 atgccgacag gcttgatgcc cttggggcaa ttggaatcgc aagggcattt agcgtaggcg
      661 gggcggaaaa ccggccaatc tacaacactg cagatccgtt ttgcaaaaaa agaaccccca
      721 acgacaagtc ttggacgact gatcattttt tccgcaagct gttgctactc gaaaagaaaa
      781 tgaacacaaa atctgcaaaa aaagaagctc aaagaagaac cagactgatg ataaagttcc
      841 tagcaaatct aaaaagagaa atctagccgt agtctatgta tcgggcgtac cttttttcta
      901 tttcgggatt ttttccctca agaatttttt ctatttcctc agtgtgccga tttttcatgt
      961 acgagtttat ctcagagaat tgttcagtgc cgggatatga ttcatagagc ctttctaaaa
     1021 cggcaagata ttctattgtc ttttggcgaa attcttctgc ggttggcttt ttgatttggt
     1081 ttagcctaaa ccatacgcac gcccagcttg ctcccactac gtgcggcagg aggtcgtagg
     1141 cccgttctat ttcaaaacgc tcttcttctt tcatttttga agcagctgtt gcgccatcat
     1201 ttttgcaaaa taggtaacaa tcatgtcggc tcctgcgcgc tttatcgaat acaaaatctc
     1261 tgtcatgaca tctgtctcgt ttatccaacc ttgttttgca gctcccttga ctagggcgta
     1321 ttccccagag acactgtatg cagaaatagg aatattgaat cgccgttttg cttccgcaat
     1381 cagatcaagg tatgccaggg ccggctttac cattacaatg tcgactccct cgtttacgtc
     1441 aagctcaatt tcccgcattg cctctctcgg gtttgtaaac ggaacctggt aggtcttgcg
     1501 gtccccaaag tgtggcgcgc agtcagccgc atctctaaac ggcgtgtaaa aagctgatct
     1561 gtgttttgca gaatgcgaca ttatgccgac ttcggtaaac ccgttttcat ctagtctgtg
     1621 tctgattgct tttacctgtc cgtccatcat tgcggacgga gagacaatgt ctactccagc
     1681 agctgcctgg ctttgcgcaa ttttagccag cgtttcgata ctggaatcat tatcaattac
     1741 atttcccttt gctatgccgc agtgtcctga tgtggtgtac tggcaaaggc agacatctgc
     1801 catgattacc atactgtcgc caaagttttt tctgacttgg gaaattgcct cctgtactat
     1861 tccattttct gcaaatgcgg atgttccttg ctcgtctttg tgttctggaa ttccaaaaag
     1921 catgattgca ggaatgccca gattctttat cgactcgact tcttttgtta attccgataa
     1981 cggcagtcgc tctattgcct ccattgactg gacctgggtt ctggacttga gtccttcctg
     2041 gacaaacact gggcagatca aatcatttgt agagagtgtt atctcctgta ctaggttgcg
     2101 catcttttca tttttgcgca gccttctcaa tcgcttggag ggaaaagagc ctagcatggt
     2161 aaaaatcgac cttgttgcaa atataatcct aggcgcgcaa aaatctactc ttttttcttg
     2221 taatcgaaaa gctttgcagc tgcctttaga agctcatcat cggactgctc agatgcccgt
     2281 cttaggttgt tcatcggctc tgagatgatt ccctcaacta ctgcctttgt cagctcgtca
     2341 ataattttga ttttttctgc gtctttttcg ccaagcatct gcagtgcctt ttgcagctcc
     2401 tttgatcttc gcacgtcaat gtccttgaat atttctttta caacgggttc tacttcgagt
     2461 cttttcatgt gggcctgaag tacctgcatc tcttcgctaa ttattttttc cgcgtttgag
     2521 cgctggcttg agcgatacct gatgtttttg tccaccattt cagctatctg atcaaggttc
     2581 atcatcttga tgccatgtat ggtagctacc ttttcgtcca cagttcttgg atttgatagg
     2641 tccaaaatca tcatgccgtt tttcttgtct ttcattgctt cgctgattct atcaaatgtg
     2701 actaaaaaat acggcgcaat ggttgcaaca aagactacat catagttgga aaagctagtc
     2761 agtatgtctt caaagctaat cgggtttcct ccaattgttt ctgcaaacgc ctttgagcgt
     2821 tccattgttc tacttgtgac aaaaaaccca taatcccttt tgattagaga ctttgcaaca
     2881 agtgttgctg cctcgccagt tccaatcagc aaaatctttt ttgattttag ctcatcaatg
     2941 ttttcttctg caagc
//
LOCUS       DASWKH010000131         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1452510, whole genome shotgun sequence.
ACCESSION   DASWKH010000131 DASWKH010000000
VERSION     DASWKH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452510"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1924
                     /locus_tag="VGA92_09420"
     CDS             <1..1924
                     /locus_tag="VGA92_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010193324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9846660.1"
                     /translation="ILHKTDVGGVKVGIDNVNDVKKTFNDMYGRLSKKKGVHVKGILL
                     EKMVPKGVELIVGLQNDPQFGPVIMVGLGGVLTEIFKDVSFRMLPISLSDAKSMLNEL
                     KGSEILKGFRGSKPIDINMLAKALVGIGKLGVDNAGHFDSIDFNPIVVYPQSYYVVDA
                     KILLRKEYKKNAISKAKPDAMYMEKFFTPKSVALVGASATPGKIGNSVLNALGKQDYK
                     GKVYPINPKQKSILGIKCYPSLDAIPAKIDLVVVCIDLAECGPIMKICAKKGIHNVVI
                     VSGGGKELGGNRAAMEAEVKELSLKHKIRVVGPNCIGMFNAANRLDCAFQGQARMIRS
                     KLGNVAFFSQSGTMGISMLESADVFGLSKMISYGNRSDVDEADMIWYAANDPQTKVIG
                     LYVEGFGDGRKFINTAKKVMNEKKKPIVIWKSGRTAAGAKQAASHTGSLGGSNAIIMG
                     AFKQAGIISVDSYQELVGVLKALAWQPPAKGNRVAMTSNGAGPMIGGIDHLERLGLTI
                     GKLSPQLLKKMKARFPPTVPIHNGNPADVGGGATADDYRFVIQQFYDEKNIDIAMPWF
                     VFQDDPLEETIVGHLADFSKKQKKPLVVGCNGGPYTEKMIKLVEKHKVPVYQDLRTWV
                     AAASALAQWGNIKNSR"
BASE COUNT          710 a          324 c          445 g          610 t
ORIGIN      
        1 aattctacac aaaacagatg taggcggagt aaaagttggt attgataatg taaacgatgt
       61 gaaaaaaact tttaatgaca tgtatggacg tctttctaaa aagaaaggag ttcacgtcaa
      121 aggaatctta cttgaaaaga tggttccaaa aggtgtagag ctaatagtag gtctgcaaaa
      181 tgatcctcag tttggccctg taatcatggt aggacttgga ggagtgctta ccgaaatttt
      241 caaagacgta tcttttagaa tgttgccaat tagtttatcg gatgcaaaat caatgttaaa
      301 cgagcttaag ggttccgaaa ttctcaaagg atttcgtggt agtaaaccca ttgacattaa
      361 catgttagca aaagccctag ttggcattgg aaagttagga gtagacaacg caggtcattt
      421 tgacagcatt gattttaatc ctatagttgt ttatccacaa tcctactatg tagttgatgc
      481 aaaaatactg cttcgaaaag agtataagaa aaatgctatt tccaaagcaa agcctgatgc
      541 gatgtatatg gagaaatttt tcactccaaa gtctgttgca ctagtaggag catctgcaac
      601 tcctggaaag attggtaatt ctgtattaaa tgcacttgga aaacaagatt acaaaggtaa
      661 agtgtatcca attaatccta aacaaaagtc aattcttgga atcaaatgtt atccctcatt
      721 ggatgcaata cccgcaaaga ttgatctggt tgttgtctgt attgatcttg cagaatgtgg
      781 gcctattatg aaaatatgtg caaagaaggg aattcataac gttgtaattg tttctggtgg
      841 cggtaaagag cttggcggta acagggccgc aatggaagct gaagtaaaag aattatccct
      901 aaaacacaaa atccgtgtag ttggtcctaa ttgtattgga atgtttaatg cagcaaatcg
      961 tcttgattgt gcattccaag gacaagcaag aatgatacgt tcaaaattag gaaatgttgc
     1021 atttttctca caaagtggaa ccatgggaat tagtatgtta gaaagtgctg atgtgtttgg
     1081 tttatcaaaa atgatcagtt atggtaatcg ctctgatgtt gacgaagcag acatgatatg
     1141 gtatgctgca aatgatcctc aaacaaaagt aattggatta tacgttgaag gctttggaga
     1201 tggacgaaaa ttcatcaata ctgctaaaaa agtaatgaat gagaagaaga aaccaatcgt
     1261 tatttggaag agtggaagaa ctgctgcagg tgcaaaacaa gctgcatcac atacaggttc
     1321 tcttggtggt tcaaatgcaa tcattatggg tgcattcaaa caagcaggaa ttatttcagt
     1381 tgatagttat caagaactag ttggagtctt aaaggcacta gcatggcaac ctcctgcaaa
     1441 gggtaatcgt gttgctatga ctagtaacgg cgctggtcca atgattggtg gaattgatca
     1501 tttagaaaga ttaggtctta caataggaaa gttgtcgcca caacttctta aaaaaatgaa
     1561 ggctcgtttc ccaccaactg ttcctattca taatggtaat ccagcagatg taggtggtgg
     1621 tgcaactgca gatgattata gatttgttat tcagcaattt tacgatgaaa aaaatattga
     1681 tattgctatg ccatggtttg tcttccaaga tgatccactt gaagaaacaa ttgttggtca
     1741 tctagccgat ttctcaaaga aacagaagaa accacttgta gttggctgta atggtggtcc
     1801 atatactgaa aaaatgataa aattagttga gaagcacaag gttccagttt accaagacct
     1861 tcgaacttgg gttgcagctg catctgcttt agcacaatgg ggaaacatta aaaattctag
     1921 ataacattta agtcctgcaa aaattcgaat aagattatgt ctcttgtaac aacctcaaac
     1981 tctgattgta tttgtactgt caaaattaac agacctgaca aacttaatgc aatgaatatg
     2041 gatgttgcaa aagaactcgt taacattttt gaaaatcttg gaaaagatg
//
LOCUS       DASWKH010000132         6843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2937413, whole genome shotgun sequence.
ACCESSION   DASWKH010000132 DASWKH010000000
VERSION     DASWKH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2937413"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VGA92_09425"
     CDS             <1..1810
                     /locus_tag="VGA92_09425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9846661.1"
                     /translation="ITLQLWKGDFIRNKLVIMCFLSVTIITIGYGEYLTTLEQEKLIE
                     QKESKIFPFVSNFETDLFDTISLLEITAKESSVTNIPFSDQIDPIFHGIREDSDIEKR
                     TIANAILLNKENLDAVFFTMPNGDMYLVEPYQYQADLATNNFAHRDWYIGVTSAKSTY
                     TSEVFQPQGKKEITVAIVTPVKSESSEIIGIWGGLVNLASWEKRFNEINFVENAKTII
                     VDHKGNRVIDSEHPSLDTIESHLYLQSVRNALAQNSGIMVETVDGVRSLVVYTPITVG
                     THTWAAIITEPHDQAFSAINFIRLLYIGIVVSIAGVMATWFLLPKKLSTTKEWWFLPQ
                     KISQIEQDVVESQPAIRSALNKKVYFVIIPLLLSSTLGVGLMQNQHPDETTNLKGSFL
                     IQNLRGDTVDTWINWKIPKGDTFHIHVVNSPEVTTERMQTISDAIYSKESVIIDDSLL
                     HKGSPGSTSTYYKGWFGAIESVSGINTRFAIPIALHTTITDKGEGHITIRLTKLQNSD
                     GYSAYTKSFVDDENHQILKSVITVYDVDSLSDDQLATIMRHELGHGLGLAHSTAPEDL
                     MAPEITTPYPYISECDIAALVGLYNGNQNSQVVCEK"
     gene            complement(1833..3686)
                     /locus_tag="VGA92_09430"
     CDS             complement(1833..3686)
                     /locus_tag="VGA92_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846662.1"
                     /translation="MDSQATESFLKKSKTEMALIVSIISITVIALAASTIGPEETNYV
                     FAIQSTILAGTMVAFSVTLIKKTKSRQELYIVLAFTAFAALWFVAGQIWNYNGIILQI
                     DQFPSIADYLWLLGYVALFVFAVQIIRPVKNALSKKLIFSICALSASFLIPSIIATVG
                     NNALANVDVIVAMLYPISDAIVLVPIICAIIIFKNTKENNFWPLFVAIILTVITDTIY
                     LFLEVSGSYYTGHLVWLGYGVANTFFVYGLYKQNQIMKKTTAPIFEQLRSEADVEKLK
                     TTISIPIVISTAVIVFVLSVVQIFGFGNLSVNEITLVQPIFYVSVSLILALSVVVSII
                     GKKYLSVKSELEKSRVEKPRLDYEVKMSPQIEVMQNRLENMGRWGRFAVRALFIISLV
                     VGAAIAFYLYSSLEQTPTILSSGRYLIEGLDGQVLDTWAIWYLAENESLHVSIVNSGL
                     LPSDKISSIQNAIISEKTIESVELTSGVYFEGWKGALAKAGLQKTTTIIPQSFVLEET
                     DAASGEIVITLSSQKPTDGVFGSTRSIADVDSHQLLKSFVTIYDVKNLDNDELASITR
                     HEMGHALGLTHSTGSEIEFYRNELNSAFISDCHIESLVLLYNEQDVSMTCQ"
     gene            3857..5707
                     /locus_tag="VGA92_09435"
     CDS             3857..5707
                     /locus_tag="VGA92_09435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX9846663.1"
                     /translation="MMQRQASFLSLGSKILIISLVSAGVFTAIIGLVDEETTVLLGNL
                     AYVPIHLAWVLTAFFSFVKLRKTQSERFLLVFLITAVLWFVADMAWTVLELVYEEDPF
                     PSIADHFYIVGYLTMFTALGIVIFPHIRKISFDVKLIAASLSLALLVPLILLTQTQEY
                     DNVASAAISYSYPVLDVILLWFCIVGALVFSKKMDFMYYLIAGLLALIVADTEFAISS
                     ISETYHVGQLSEVFFYWSYILLAFAAYRVYKSPLQKPSEIQLRTIMISGKTRIALVVF
                     TFVLLVGIIVSAIAYFGFTNFSPNEERVIIPSLYASSFAVFLTIVTHAAFHKKKTNLV
                     YKLERIETLAQPTDQLVLLQKQISRLEAATKKNTMLTIIGMTVLGGIMLFYVSTNFIE
                     GPSYQTELVSGKYFIENLKGDRIDTWVAWQISKDKTIQVTVINSAELSQEKLKAINDP
                     ILSESSIVLENAFMNKDPPNETSLYHLGWKGALSSISKIGTKYNVPTNFQVDVSEKSV
                     GDIVIILSTAKETDGSLGFTRSIADENTNQILKSFITIFDVDNLDGNELSAIVRHEFG
                     HAMGLGHSTDEMDLMYPEFSSNQAFISECDLDAMASLYNGEKISQVVCRH"
     gene            5719..>6843
                     /locus_tag="VGA92_09440"
     CDS             5719..>6843
                     /locus_tag="VGA92_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX9846664.1"
                     /translation="MKTSFWFVMGLIMSLFFPAAALPPDAFAQPDYNDEIIEEMQYWY
                     FDRIEKSEITIIGEPDLKDGLVGKALGLDGKNGSIIVENQNKGKELSRFTVSAWLKPE
                     YTKSPKDFVVLSSENAFELSVLNLQDKRTARFSIFDGFRWISVETRTKIDDGDWTNLT
                     AGYNGTALNIFVNGRLDGSTPVFDTIRYFAYGREVTKFDYSNVRNPVLSADLIIGAKK
                     IQGRITDMFSGQLDEIQFYFTAMTVDQAVPKLKTKNNYMLISDNCTQRKSAQCSIQMS
                     LITGEIIDDKSQIMFDHGTITINEIEYLIKSKDWRGTIPVKPGQATFSGHAVDSDNNE
                     IFVVIVGYFVENTMTGHLYRMSGSIKGSNIQTDLVGNFELV"
BASE COUNT         2151 a         1362 c         1365 g         1965 t
ORIGIN      
        1 gatcacatta caactgtgga aaggtgattt cattagaaat aaactagtca taatgtgctt
       61 cctttcggtc acgattatca ccatagggta tggcgaatat cttaccacat tagaacaaga
      121 aaaattaatc gaacaaaaag aatccaaaat ttttccgttt gtttcaaatt ttgaaacgga
      181 tctttttgac acaatatcac tcttagagat cactgcaaaa gaaagcagtg tcaccaacat
      241 accattttca gaccagattg atcccatctt ccacggaatc agagaagact cggatataga
      301 aaaacgtaca attgccaatg ccatactttt aaacaaagaa aacttggatg ccgtgttttt
      361 tacaatgccc aatggggaca tgtatttggt tgaaccatac cagtatcaag cggatcttgc
      421 cacaaacaat tttgcacaca gagattggta cattggtgtg acaagtgcaa agtcaacata
      481 cactagtgag gtgttccagc ctcagggaaa aaaagaaatc acagtcgcaa ttgtaactcc
      541 cgttaaatca gagtcatcag aaatcatagg aatatgggga gggttggtaa atcttgcttc
      601 ttgggaaaag cgatttaatg aaatcaattt tgtagaaaac gcaaagacca taatagttga
      661 ccataaaggg aatcgtgtca tcgattcaga acatccatcg cttgacacaa tagagtcaca
      721 tctttacttg caaagtgtaa gaaatgcgct tgcacaaaac agcggcatta tggtagagac
      781 ggtagatggt gtaaggtcat tagtagttta caccccaatt acagttggca cgcacacttg
      841 ggcggcaatc ataacagagc cgcatgatca agcgttttca gccatcaatt ttattcgcct
      901 actatacata ggaattgtag tatcgattgc cggagtaatg gcaacttggt tccttttgcc
      961 aaaaaaatta agcactacaa aagaatggtg gtttctcccc caaaaaatta gccagattga
     1021 gcaagatgtt gtagaatcac aaccagcaat acgcagtgca ttaaataaaa aagtgtattt
     1081 tgtaataatc ccgttactgc tatcatcaac tttgggcgtt ggcctgatgc aaaatcagca
     1141 tcctgatgag acaacaaatc tgaaagggtc atttctaata cagaacttga gaggagatac
     1201 ggtagacacg tggattaatt ggaagatacc aaaaggcgac acatttcaca tccatgttgt
     1261 aaactcgcct gaggtaacaa cagaaagaat gcaaacaata tcagatgcca tctattcaaa
     1321 agaaagcgtc ataattgatg attccttatt gcataagggt tcaccgggct caacttcgac
     1381 atattacaaa ggctggtttg gggcaataga aagtgtttct ggtatcaata caaggtttgc
     1441 aatacctatt gctcttcata ctaccattac tgataaggga gagggtcaca ttactataag
     1501 gctaactaaa ttgcaaaatt ctgatggtta ttctgcatat actaaatcat ttgttgatga
     1561 tgaaaaccat caaatcctca agtcagtaat aacagtctat gatgtggaca gtctgagtga
     1621 tgatcagctt gcaaccataa tgcggcatga gcttggacat gggttaggcc ttgcccactc
     1681 gacagccccg gaggatctca tggctccaga gataacaacc ccatacccat acatttcaga
     1741 gtgcgacata gctgccctcg ttggcctata caacggtaat caaaacagtc aggttgtttg
     1801 cgaaaaatag accaaaccaa attaattatt tattactgac aagtcatgct gacatcttgc
     1861 tcattataca gtaacactaa agactctatg tgacagtcag atatgaacgc agagttcaac
     1921 tcatttctat aaaattcaat ctcacttcct gtagaatgag tcagcccaag tgcatgaccc
     1981 atttcatgtc ttgttatgga ggccagctca tcattgtcaa ggtttttcac atcataaatt
     2041 gttacaaatg attttaggag ctgatgcgag tccacgtctg caattgatcg cgtcgagcca
     2101 aacacaccat cagtcggctt ttgggaagaa agtgttatga ctatttcgcc tgatgctgca
     2161 tctgtctctt caagtacaaa agactgaggg attatagtag ttgttttttg aagacctgcc
     2221 tttgctagtg ctcccttcca cccttcaaaa taaacccccg atgttaattc aacggactca
     2281 attgtttttt cagagatgat ggcattttga atagaactaa ttttgtcact tggcagcaag
     2341 ccagaattta caatactcac atgcagtgat tcgttttctg ccaaatacca aattgcccac
     2401 gtatccaaaa cttggccatc caatccctca atgagataac ggccagatga cagaattgtc
     2461 ggagtttgct ccaggcttga ataaaggtag aatgctattg cagcgccaac taccagactt
     2521 attatgaaga gtgcccggac tgcaaatcga ccccatcttc ccatattttc gagcctgttt
     2581 tgcataactt ctatctgcgg cgacattttg acttcgtagt ccaggcgtgg cttttctact
     2641 cgtgattttt ctagttccga ttttacggac aaatattttt tgccaataat tgataccact
     2701 acagaaagtg ccaaaattag cgaaacactc acataaaaaa tcggctggac tagtgtgatt
     2761 tcatttacag aaaggttgcc aaacccaaaa atctgaacca cggataatac aaaaactatg
     2821 actgcagtag aaatcactat tgggattgag attgttgtct tgagtttttc aacatctgcc
     2881 tcagatctta actgttcaaa tattggtgcg gtggtttttt tcatgatttg gttttgtttg
     2941 tataacccat atacaaagaa agtattggca accccatacc caagccatac aagatgacct
     3001 gtgtagtatg agccactcac ttcaagaaac aaatagattg tatcagttat tacagttaga
     3061 attattgcca caaacaatgg ccaaaaatta ttttcctttg tgtttttaaa aataataatg
     3121 gcgcaaatta ttggaacaag tactattgca tctgatattg ggtagagcat tgcaacaatg
     3181 acgtcaacgt ttgcaagagc attgttacca accgtagcaa ttattgatgg gataagaaat
     3241 gaggccgaaa gcgcacaaat ggaaaaaatt agtttttttg aaagagcatt tttgactggc
     3301 cttattatct gaactgcaaa gacaaacaat gcaacatacc caagcagcca caaatagtct
     3361 gcaatggatg gaaattgatc tatttggaga attatgccgt tgtagttcca gatttgtcct
     3421 gcgacaaacc acaatgccgc aaacgcagta aaggcaagta ctatgtaaag ctcttgtcgg
     3481 ctttttgttt tttttatcag agtgacggag aatgcaacca tcgtacctgc aagtattgtt
     3541 gactggatgg caaaaacata attcgtctct tccggaccaa ttgtgctagc tgcaagagct
     3601 atgacagtaa tactgataat cgatactatc aaggccatct cggtctttga tttttttaaa
     3661 aatgactcgg ttgcttggct gtccataccc aaggaaaatt actggttttt tattataata
     3721 agggtcaaaa tatcataatt gataatgcag ttctttattt taggctcaag tactacctac
     3781 cagaattgat aatttcgttc ttggtttaat acataaaaaa ctactttctt atttgatgag
     3841 tgtttgtagt aataaaatga tgcagcggca agcctccttt ttaagcctag gcagcaaaat
     3901 cctcataatt tccctggttt ccgcaggagt attcactgca atcattggcc tcgtagatga
     3961 ggaaactact gtcttacttg gaaatttggc atatgtgcca atacatttgg cgtgggttct
     4021 gacggcattt ttttcctttg taaaactgcg aaaaacccag tctgaaagat tccttcttgt
     4081 gtttttgata actgcagtat tgtggtttgt cgcagatatg gcctggactg tactggagct
     4141 tgtatacgaa gaagatccat tcccgtcaat agctgatcat ttctatattg tcggctacct
     4201 gacaatgttt actgcacttg ggatagtgat ttttccacat ataagaaaaa tttcttttga
     4261 tgtgaagtta attgccgcct cgctgagtct tgcactttta gttccgctaa ttttgttaac
     4321 ccaaacccaa gaatatgaca atgtcgcatc agctgccata tcctacagct atccagtcct
     4381 tgatgtcata ttgttgtggt tttgtatagt aggcgcactt gttttttcca aaaaaatgga
     4441 ttttatgtat tacttgattg ccggtctgtt agctttgatt gttgccgata ccgagtttgc
     4501 aatctcgtcc atatctgaaa cgtatcatgt cggtcagctt tctgaagtct ttttttactg
     4561 gtcctacatt ctgcttgcat ttgctgcata cagagtatac aaatcacccc tccaaaagcc
     4621 atcagagatc caactaagga cgataatgat atctgggaaa acaagaattg ccctcgtagt
     4681 ttttacgttt gtgttgcttg tgggaataat tgtatcagcc attgcgtatt ttggatttac
     4741 aaactttagc ccaaatgagg aaagagtgat catcccatcg ctttatgcaa gctcctttgc
     4801 cgtttttcta accatagtaa cacatgccgc atttcataag aagaagacaa atctagtgta
     4861 caaattagaa cggattgaaa cccttgcaca gccaactgat cagcttgtac tgcttcaaaa
     4921 acaaatcagc aggctggaag cagccactaa aaagaacacc atgctcacca tcattggcat
     4981 gactgttttg ggcggaatta tgctatttta tgtttcaact aacttcatag agggaccatc
     5041 atatcaaacc gaattggtat ctggcaagta ttttattgaa aacctgaaag gagatagaat
     5101 tgacacatgg gttgcatggc agatttcaaa ggacaagaca attcaggtaa ccgtaattaa
     5161 ttcggcggaa ctgtcgcaag aaaagctcaa agcaattaat gatccaatcc tatctgagag
     5221 ctcgattgtt ttagaaaatg cgtttatgaa taaagacccg ccaaacgaga catcgctgta
     5281 tcatctcggg tggaaaggcg ctcttagtag catatctaag attggcacaa aatacaacgt
     5341 acctacgaat tttcaggtgg atgtttctga aaaatcagta ggcgacattg tcataatttt
     5401 atccaccgca aaggagacag atggctcact tggattcaca cgctccattg ccgatgagaa
     5461 cacaaaccag atcctcaaat catttatcac catttttgat gtagataacc tagatggtaa
     5521 tgagctgtcc gccattgtaa gacacgagtt tggccacgcc atgggacttg gacactcaac
     5581 ggatgagatg gatctaatgt atccagaatt cagctcaaat caggctttca tctcagagtg
     5641 tgacttggat gccatggctt ccctgtataa tggcgaaaag atatcgcaag ttgtgtgcag
     5701 gcactaagta gattgagatt gaaaacttca ttttggtttg taatgggatt aattatgtca
     5761 ttatttttcc cagctgcagc acttccaccg gatgcctttg cccaaccaga ctacaatgac
     5821 gagatcatcg aagagatgca gtactggtat tttgatagga tagaaaaatc cgaaattacc
     5881 ataattggtg agcctgacct taaggatggt ctcgtgggaa aggctctagg tcttgatggg
     5941 aaaaatggct ccatcatagt ggaaaatcaa aacaagggca aagaactctc cagatttacg
     6001 gtctcagcgt ggctaaagcc agaatatacc aaatctccaa aggattttgt tgtgctgagc
     6061 agcgaaaatg cctttgagct gtcggttctg aacctgcaag acaagcgcac agcaagattt
     6121 tcaatatttg atggctttcg ttggatttct gttgaaacta ggaccaaaat agacgacggg
     6181 gactggacaa accttactgc aggctacaac ggcacagctc tgaatatttt tgtcaacgga
     6241 aggctcgatg gcagcacacc agtctttgat acgattcgtt attttgcgta cggaagagag
     6301 gtaaccaagt ttgattacag caatgttcga aatcccgtac tctcagctga cctcattatt
     6361 ggggcaaaaa aaatccaagg tcggattaca gacatgtttt ctggccagct tgatgaaatc
     6421 cagttttatt ttaccgcgat gacagttgac caagcagtac caaagctaaa aacaaaaaac
     6481 aactacatgc tgatttcaga taattgcaca caaagaaaat cagctcaatg tagcatacag
     6541 atgtcattaa tcactggcga aattattgat gacaagtcac agataatgtt tgatcacggt
     6601 acaattacaa ttaatgaaat agaatatttg ataaaatcaa aggattggag ggggaccata
     6661 ccagtcaaac cagggcaagc tacattttct ggacatgctg tagattccga caacaatgag
     6721 atctttgtgg taatagtagg atattttgta gaaaacacca tgaccggaca cctatacaga
     6781 atgtcaggca gcatcaaagg aagcaacatc cagacagact tggttggaaa ctttgagctg
     6841 gtc
//
LOCUS       DASWKH010000133        12395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1095768, whole genome shotgun sequence.
ACCESSION   DASWKH010000133 DASWKH010000000
VERSION     DASWKH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 12395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12395
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095768"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(778..1011)
                     /locus_tag="VGA92_09445"
     CDS             complement(778..1011)
                     /locus_tag="VGA92_09445"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9846665.1"
                     /translation="MTWQVVWSEKSVKQLEKIDKKDAQRIYDHVLDCSNNPFRTAVRL
                     VGSPFFRLRVGNYRVILDLQQSKLVIFVVEVDL"
     gene            complement(1013..1243)
                     /locus_tag="VGA92_09450"
     CDS             complement(1013..1243)
                     /locus_tag="VGA92_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846666.1"
                     /translation="MYTSIQIEKEIKARLDKLKNHPRETYNEVLVRLLKIAQDDDILS
                     PAVIKNIEEGIADIKAGRVYSSAQVKKKLGLK"
     gene            complement(1454..1828)
                     /locus_tag="VGA92_09455"
     CDS             complement(1454..1828)
                     /locus_tag="VGA92_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9846667.1"
                     /translation="MIRTKPTTKAMSDGPLESMFPVASSKILDFLSTHKRWDYSVSDI
                     AKYSGISFKTALIEVKKLEAQDVVVKTRDVGNATMYQFNMDSRQAQYIDKLINEIATR
                     RIQEVLEPKPTMKIKQKKKKSK"
     gene            complement(2314..2412)
                     /locus_tag="VGA92_09460"
     tRNA            complement(join(2314..2359,2375..2412))
                     /locus_tag="VGA92_09460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2376..2378),aa:Ser,seq:gga)
     gene            complement(2480..3571)
                     /locus_tag="VGA92_09465"
     CDS             complement(2480..3571)
                     /locus_tag="VGA92_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX9846668.1"
                     /translation="MAKKIKVGLVGIGNCFSGLIQGIEYYRQKPSQQVIGIMHNKIGS
                     HSIHDLDFVAGFDVGTNKVGKTINEAIYAYPNMVDWVPKNKMPKTKSLVYESPALDGV
                     GIWVENRVNPIKSKKSKEQITKEIKKTIEKTGAEVIVSYLPVGSEMATQYWAQICLDT
                     NSAFVNCIPSFIASNKKWGQKFADKKLPVIGDDIKGQVGATIVHRTLARLCDERGTKI
                     EKTYQINVGGNTDFLNMKEQERLVSKRISKTESVQSQLSQRLADDQIYVGPSDFIPFL
                     GNTKLMFMRIEGRQWANIPYNMEVRLEVDDKANSAGIVIDAVRLAKLALERKLGGPVI
                     PACAYLMKHPPKQMSDPQAKTELEKFIKP"
     gene            complement(3611..4048)
                     /locus_tag="VGA92_09470"
     CDS             complement(3611..4048)
                     /locus_tag="VGA92_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846669.1"
                     /translation="MIPKSLVIAVIMGVSAAAALGIYFVKLGSQDLEFVQGPAISVLV
                     EKQDYKVGEKITIGIINTGTTEITFSSEFPSLRIRALDGTVFFSTSFDGMQLTPNQKH
                     VFEWEQLKNDSSKIIEGRYVIDSFAYNPDKQKISDSVTVNILR"
     gene            complement(4045..5043)
                     /locus_tag="VGA92_09475"
     CDS             complement(4045..5043)
                     /locus_tag="VGA92_09475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HEX9846670.1"
                     /translation="MTLPQLQNLVVIDQDVTNDQHGVYYDKRSIKELLNYGLILLDKP
                     PGPTSHEAVAWAKRILKLPKIGHSGTLDPQVSGVLPLGLGEATKALGVLLLGPKEYYG
                     LGRLHSLPGKEKLDSVLNMFKGEIYQKPPQRSSVLRQTRTRTIYELEVIEQKERLLLL
                     RVLCEAGTYIRKLFYDIGEILGPGATMIELRRSRVHQFNEGQKLVTLHELADAFAQWQ
                     EKNDDSKLYKMILPIEYALTEIKAVVIRDSAVDALCHGAQLAIPGILQISPNLSKGNL
                     VAIYTQKGEVVALAESLLSEQEIKDATKGYAFQTKRIIMAPGTYPKSWHSKTTTTK"
     gene            complement(5040..5600)
                     /locus_tag="VGA92_09480"
     CDS             complement(5040..5600)
                     /locus_tag="VGA92_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX9846671.1"
                     /translation="MPKSIIISGPPAIGKTTISRGLAKEFGLKSLSGGDVLKELAEEQ
                     GFKTQGDDWWDTKDGMKFLSQRKDNYEFDNKVDEKLKKIFLEGNVVITSYTLPWLVKG
                     GIKIWLAGSHENSAKRMQSRDNMSFQEAFDIVKRRYDENKILYKKLYGFDFGDDLKVF
                     DVVINTDGLDAQTVLNTAIQKVRELI"
     gene            complement(5603..6220)
                     /locus_tag="VGA92_09485"
     CDS             complement(5603..6220)
                     /locus_tag="VGA92_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HEX9846672.1"
                     /translation="MEPIIILQFLESIFLQGDFFGLHQGPLGSADPFIKGMIPSMFGI
                     LGFAILLNLFNAAVRKKLVNQEKLKRIMKETRAWQKERMAAFRAKDTEKQAELNKKSA
                     YMSRLNQELMQMNMRPMMITFVPLILIFYFVLPPLFSYTVALSPISLNVIPGDFFQLT
                     CTADKIDGKICHSVNDIYFWAWYFLGSIAFSGMIMKLTKTSMDLT"
     gene            complement(6222..6800)
                     /locus_tag="VGA92_09490"
     CDS             complement(6222..6800)
                     /locus_tag="VGA92_09490"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9846673.1"
                     /translation="MGESKKVVIVGIAGVGKTTLVSKIVEILNSKKKSVSVHSFGTVM
                     FEEAKKLGIENRDELRKLSVEEQKELQKMAAKKIASFNHNIVIIDTHAFISTKEGFYP
                     GLPSHVLEELKPANLITVSARPEEIYNRRMKDTTRERDIISIDSIKKELAVQEAMLSS
                     CAVITGSPMKSILNSEGKVEEAANEVINAIGL"
     gene            complement(6787..8217)
                     /gene="secY"
                     /locus_tag="VGA92_09495"
     CDS             complement(6787..8217)
                     /gene="secY"
                     /locus_tag="VGA92_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9846674.1"
                     /translation="MAEGTATGIVKRIVEKAEPYLVQVPKPKKKLSLQTRLLWCGIAL
                     LIYQVMGQTPLFGATAPEFDFLQFARVIFASQQGTLVELGIGPIVTAGLLMQLLKGSE
                     ILKFDFTKPEERGVFQTATKLVTYIVIIIESVVYAIAVYGPGIANPTVLYVIIGQLIA
                     ASIIIMFLDELIQKGWGLGSGISLFIMAGVAQQILWSIFSPLYAGDGSHIGVFPFVAQ
                     SIMQGDITEIFFRANQLPSIFGLFITAGVMLLLVYTQAIKVEIPIVSTKYRGFSATYP
                     IKLMYVSNIPVILASALTANAIFMGQMLWANVNPRNNNPLFNILAQFDPTAPSTPIGG
                     IVYYITPPRGLDLAALDPMRAVLYIFFMIGIVIIFGRLWVELGGLSPKSAAKNLLDAD
                     VQIPGFRKSNQPVEALLNKYIPSVTIIGSAILGALAGVSDVLGVFGSGIGILLMVDIL
                     INYYQQLVREQVEVVMPRLGALLGRK"
     gene            complement(8210..8632)
                     /locus_tag="VGA92_09500"
     CDS             complement(8210..8632)
                     /locus_tag="VGA92_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HEX9846675.1"
                     /translation="MATRLRKTRKLRGSRTHGWGQIGQHRASGHKGGLGKSGMHKHLF
                     SSMLKYDPDHFGHDSTHPPHPNITRKWASVRDLDDFFAKYGKQEGGKKVLDLDSLGYD
                     KLLGGGQLKNAYSVKIRQYTASAEEKIKKVGGEVLAVG"
     gene            complement(8633..9100)
                     /locus_tag="VGA92_09505"
     CDS             complement(8633..9100)
                     /locus_tag="VGA92_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX9846676.1"
                     /translation="MPKAYLVVRMRGQINVPHWVKTTLNLLKLEKKFRATIIPSKDNT
                     LGMLDRVKHYVSWQEANLDITKELLDKKARKTGYKKITNDDLTKLGFKTIDELAASIT
                     EGKISLNKINPIKPWFALSPPRRGFKRSTRKTYGEGGILGQNKELIELVRNMI"
     gene            complement(9107..9754)
                     /locus_tag="VGA92_09510"
     CDS             complement(9107..9754)
                     /locus_tag="VGA92_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX9846677.1"
                     /translation="MSQTAAERPRHDRRSSRKEEEVWVPRTELGKKVFAGLITTMDEI
                     YANGLRIQEAGIIKKLLPDLKTEVIDVGMVQTMTPNGQRTKFKAMVAAGNENGWLGIG
                     QGKSKQMRIAIEKATTNAYLNVSPVKLGCGSWECRCDQSHSVPFKVRGKGGSVVVEIV
                     PAPRGLGLVAGGKIRNLLKLAGLKDAWTTAKGSTETMNSTSKAVLQCLRQTFSQG"
     gene            complement(9751..10239)
                     /locus_tag="VGA92_09515"
     CDS             complement(9751..10239)
                     /locus_tag="VGA92_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX9846678.1"
                     /translation="MAYSTILRRLREEKTNYKKRKLLLMSRRDFITVQISNENTLVQI
                     HKPQMSGDKVVASAHSRFLAQKGWKGSRKNVPAAYLTGYLAGKKALSKGSNDVILYSG
                     TRRYTQRMAAALKGVIDAGLQIPADEEALPSQNRINGDHLKIKNDVAKFKSIIDGEVK
                     QK"
     gene            complement(10270..11184)
                     /gene="argF"
                     /locus_tag="VGA92_09520"
     CDS             complement(10270..11184)
                     /gene="argF"
                     /locus_tag="VGA92_09520"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9846679.1"
                     /translation="MIKVKNVLTLGELDKKEVLQIIDLAIKLKKDLKKGITKPLLKNK
                     TLAMIFQKPSTRTRVSFEVGMFQLGGYALTLSPQDLQLSRGETVEDTARTLSRYVDIM
                     MARVYEHSTIDVLAKNSTVPVINGLSNSFHPCQILADLMTIKERKGKLKGLKLAWVGD
                     GNNVCNSMLYGCSKVGISMSIATPKGFEPIANVVKDCKKIIDIELTQDPVKAVKDADI
                     VATDTFVSIHDDPKRFGKFFPKYQVNSSLMSKSKKGSIFMHCLPAKRGQEVTASIIDG
                     PQSVVWDEAENRLHSQKALLSMLALEHL"
     gene            complement(11181..>12395)
                     /gene="ftsY"
                     /locus_tag="VGA92_09525"
     CDS             complement(11181..>12395)
                     /gene="ftsY"
                     /locus_tag="VGA92_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9846680.1"
                     /translation="TAFSSVAKSLSERELKEGDIDDVLFQLEIALMESDVATEVIDSI
                     KSDLKKKLVGTSIEKKEIEEFVKNNLIQNISNLFDTAGTIDILSKINSKKSSSEPFII
                     LFVGINGTGKTTSLAKLAYLLKENKFSIAIAAADTFRAGAIEQLKEHADRLNLKIIAQ
                     NYGADPAAVARDAVLYAKSHKIDCVLIDTAGRMQTSKNLMEQIEKITKVVKPDLKIFV
                     GDSLAGNDTVSQAREFYQHVEFDGAILTKSDADARGGAALSVVKITSKPVLYVGVGQE
                     YPDLKPFDKKLFLQTVFGESTLPKPELKPEPELKPEPKKNKDPFEGINDKDISRYADL
                     YDVPPPESDDEAFVLAQKIKKWIENGRPKLGEQAKEEKKKPEPSEGEEEKKVKEEKDA
                     KPKKGLFGWRKK"
BASE COUNT         3165 a         2621 c         2372 g         4237 t
ORIGIN      
        1 ctaagtggca agatatatag caacattttc aaaaaacaaa tccaatttca tgcaaaaaat
       61 tgattttttc atattgtaag atgttgtgtt tttacatatc ttacaacttc aaagtgttaa
      121 attttattgt aaatgtaacg ccataattca ccacactctg atcatgtttt atagaatgaa
      181 tcaaaaaaaa ttgttaaatc aaaattaatt ttgggaatga ttttgtctat gattttattt
      241 ggttctgtaa cattaggtga gatgccagct gttgcagcaa caaaagcaaa aggcacccat
      301 gtttccaaat agggttcggc tacagcaaga acagtatgcg gagatcatct ttgtgaatct
      361 ggagaaacat atgatttaag agaaaagata cgaaattaca ttcttggaga tttaaaatgc
      421 agcgtgtttc cagaagaacc tgaatgtata gacatggcaa cagcatcaca gcaagcggtt
      481 caaatcaagc ttcctttttc aagtgtagcc tgcaaagccc ttaagacaaa atatgacagt
      541 ctaactttga ctgaaatagt gaaactaaga aagttgtgca aaggaataac ggctcaaact
      601 cctagtgatt gtcaagtagg cgaacgttgg agttcacgtc atggatgtgt accagatctt
      661 agtgtcctag gaccatttga tccttactaa tgatattcga tacaacgcga tgtgtttacc
      721 acatccaccc tcacatctat aaaaacatct tggatgattt tatttgtaaa ttctttttca
      781 aagatctact tcaactacaa aaataaccag cttgctttgc tgcaagtcta aaatgacgcg
      841 ataatttcca acacgtagcc tgaaaaacgg cgaaccaacc aacctaactg ctgtcctgaa
      901 cggattgttt gagcagtcaa gaacatgatc atagatcctt tgggcgtctt ttttgtcgat
      961 tttttctaat tgttttacgg atttttcaga ccagactacc tgccacgtca tcttacttta
     1021 gtccgagttt ctttttgact tgagctgagc tatagacacg acctgccttt atgtctgcta
     1081 ttccttcctc tatgtttttg attactgcag gacttagtat gtcgtcatcc tgggcgattt
     1141 ttaggagtcg gacaagaacc tcgttatagg tctcacgggg atgatttttg agcttgtcaa
     1201 ggcgcgcctt aatctctttt tcgatttgta tactagtgta cattatagta tattataaga
     1261 tactataaca taaaaacata atgttcaatt tcattcaaac tttaaagaaa taatgaaagc
     1321 gtcgggagag agatctgtca atttggatta acatcctctc ttatagctat aaaaacctca
     1381 tgcgtgtaag gaagtttttc tccatttact ggttagaatt cataaacagc gtctaggtat
     1441 accttaatcc tgcctacttt gattttttct ttttctgttt aattttcatt gttggttttg
     1501 gttccaagac ttcttggatt cttcttgtag caatctcgtt tatgagcttg tcaatatatt
     1561 gtgcttgtct tgagtccatg ttgaactggt acattgtggc attaccaaca tcccttgttt
     1621 tcacaactac atcctgtgct tccagttttt tgacttctat cagtgcggtc ttaaatgaga
     1681 ttccggaata ttttgcaata tcggacaccg aatagtccca tcgtttatgt gtggaaagaa
     1741 agtcgagtat ctttgagctt gcgactggaa acatgctttc aagcggaccg tcagacattg
     1801 ctttagtagt tggttttgta cggatcatga tacatgttag ataaaatatt atattaaact
     1861 tactagatct atgtaatcta gtacataatt aataaataca tcaaccacct aagctttgtt
     1921 acggtcaagc agagcatttc aacattcatt ttggaaaaga tgtatagaaa tagaatcata
     1981 ggtgggagac acatctctct tgaagatctc agaactggat gcccttcaca tgagcgagga
     2041 aatgttgaaa aaactctaaa aaaattagta aaagagaact tggttgtgca acatcctacc
     2101 tcatatggaa tgcagtatgc acttaatcct caacgaatag acgaaattat gaaaattttg
     2161 aacattagat agatgatata aaagtgtatt aaaagaacct caaaatactg taatggtggg
     2221 cccacaggga tttgaactcc cagttcaacg cccaacaaac tttttcaggt tttcaaaaaa
     2281 ttaggttcag actttataaa tgaaaaataa atgcgccggg agagagattt gaactctcga
     2341 acccttgcga gtacgcgctt tatggttttt caaattccag gcgcgcgccc taccaggcta
     2401 ggcgaccccg gcaaatgtcc tttggacaaa aaccatacca gaaattgact atatagaggt
     2461 gtttttgaaa tcagaaaaat tacggcttga tgaatttttc cagctctgtc ttggcttgag
     2521 gatcactcat ttgttttgga gggtgcttca tcaaataagc gcatgcagga atgactggcc
     2581 ctcctaattt tctctctagt gctagctttg caagacgcac tgcatcaatt acaattcctg
     2641 ccgagtttgc cttgtcgtcc acttcgagtc gcacttccat attatatgga atgtttgccc
     2701 actgcctacc ctctattctc ataaacatca attttgtatt gcccaaaaac ggaataaagt
     2761 cagacggccc cacatagatt tgatcatctg ccagtctttg cgagagctgg ctttgaacac
     2821 tttctgtttt tgaaatcctt ttgctgacta gtcgttcctg ttctttcata ttcaaaaagt
     2881 cggtgtttcc cccgacattg atttggtaag tcttttctat ctttgttccg cgctcatcac
     2941 acagtctggc aagagttctg tgtacaattg ttgcaccaac ctggccctta atgtcatcgc
     3001 ctattactgg cagttttttg tctgcaaatt tttggcccca ttttttgttt gatgcaataa
     3061 acgacgggat gcagtttaca aatgcagaat ttgtgtcaag acaaatttgt gcccaatatt
     3121 gtgttgccat ctcagaaccc acaggaaggt aagacactat tacttcggct ccggtttttt
     3181 cgattgtctt tttaatctct tttgtgattt gttcttttga ttttttgctc ttaattgggt
     3241 tgaccctgtt ttctacccag attcccacgc catcgagtgc cgggctttcg tatactaggg
     3301 actttgtttt tggcattttg ttttttggaa cccagtcaac catgtttggg tatgcataaa
     3361 ttgcctcgtt gattgttttg ccgaccttgt ttgttcccac atcaaagcca gctacaaagt
     3421 ctaagtcgtg aatcgaatgg ctgccaatct tgttatgcat tattccaatt acttgctgag
     3481 atggcttttg cctatagtat tctattccct gtataagacc tgaaaaacag ttccctatac
     3541 caacaagacc cactttgatc ttttttgcca aacctaccct gtggtttcgt tgaactcgtt
     3601 ttaaattttc ctacctgaga atgttaacag ttacactatc actgattttt tgtttgtcag
     3661 gattgtaagc aaatgaatca attacatatc gtccctctat gattttggag ctgtcgtttt
     3721 tgagttgttc ccattcaaag acatgctttt gatttggcgt tagttgcatt ccatcaaagc
     3781 tcgtggagaa aaagaccgtt ccatccagtg ccctaattcg caagcttgga aactcgcttg
     3841 agaaggtgat ctcagttgtt cccgtattta ttatcccaat tgtgatcttt tctcctacct
     3901 tgtagtcctg tttttcaact aaaacagaga tggctggtcc ttggacaaac tccaaatctt
     3961 gcgagccaag ttttacaaaa taaattccta atgcggcagc tgcagacact cccatgataa
     4021 ctgcaattac taagcttttt ggaatcattt tgtagtggta gtttttgagt gccagctttt
     4081 agggtatgtg cctggcgcca tgattattcg cttggtttga aatgcatagc cctttgttgc
     4141 gtctttaatt tcctgctctg atagaagtga ttctgctagt gcgactactt cgcctttttg
     4201 ggtgtatatt gcgactaggt ttcctttgga aagatttggc gagatttgga ggatgccagg
     4261 tattgccagt tgtgcgccgt ggcataatgc atcgacagcg gaatctctaa ttacaactgc
     4321 tttgatttca gtcaatgcat attcaattgg gaggatcatt ttgtatagtt tagagtcgtc
     4381 atttttttct tgccattgtg caaacgcatc tgcaagctcg tgtaaggtca ctagcttttg
     4441 gccctcgttg aactggtgaa ctcgggagcg acgcaactca atcattgttg caccaggtcc
     4501 aagtatttca ccgatatcat agaatagttt acgaatgtag gttcccgcct cgcacaaaac
     4561 gcgcaataat aaaagccgct ctttttgttc tattacttct agctcataga tggttcgtgt
     4621 tcttgtctgt cgtaagacag atgatcgttg tgggggtttt tgataaattt ctcctttaaa
     4681 catattcaaa acactatcaa gtttttcttt gcctggaagg gagtgcagtc ttcccaaacc
     4741 atagtattct tttggaccaa gcaataagac gccaagtgcc tttgttgcct cgccaagccc
     4801 cagtggcagg actccagata cttgcggatc cagtgttcca ctgtgaccaa tttttggaag
     4861 ttttagaatt ctttttgccc aagcaactgc ctcatgactt gtcggtcctg gcggcttgtc
     4921 taaaagaatc aagccgtaat tgagcagttc tttaattgat cttttatcat aatacactcc
     4981 atgttggtcg tttgtcacat cttgatcaat tacgacaaga ttttgtagtt gtgggagagt
     5041 caaatcagtt cccttacctt ttgtattgcg gtgtttagga ctgtttgagc atctaatccg
     5101 tccgtattta tgacaacatc aaatactttc aagtcgtccc caaaatcaaa gccatagagt
     5161 tttttgtaca ggattttgtt ttcgtcgtac cgtcttttta ctatatcaaa tgcctcctga
     5221 aatgacatgt tatcgcgtga ctgcatccgt tttgcgctgt tttcgtgcga gcctgcaagc
     5281 cagatcttta tgcctccttt gacgagccac gggagagtat agcttgtaat gactacgttt
     5341 ccttcaagaa atatcttctt tagtttttca tctactttgt tgtcaaattc atagttgtcc
     5401 tttctttggc tcagaaactt catgccgtcc ttagtgtccc accagtcatc gccctgtgtt
     5461 ttgaatcctt gctcctctgc tagttccttg agcacgtctc caccgcttag gctttttaga
     5521 ccaaattcct tggcaaggcc cctagaaatt gtagtcttgc cgattgctgg aggaccggaa
     5581 ataattattg actttggcaa tgctaagtta aatccattga tgtcttggtc agcttcatta
     5641 tcattccgct aaatgcgatt gaccccagaa aataccaagc ccaaaaataa atatcgttta
     5701 cactatggca aatctttcca tcaattttgt cagcggtgca cgtcagctgg aaaaagtctc
     5761 ctggaattac atttaatgat attggtgata atgcgacagt atacgaaaat agtgggggca
     5821 ggacaaagta gaaaatcaga atcagtggta cgaacgttat catcattggc ctcatgttca
     5881 tctgcatcaa ttcctggttc agtcttgaca tgtatgctga ttttttgttg agttctgctt
     5941 gtttttcagt gtcttttgcc ctgaatgcgg ccattcgttc tttttgccat gcccgtgtct
     6001 ctttcattat tcgcttgagt ttttcttgat taactagctt ttttctgact gccgcattaa
     6061 acaagttaag aagaattgca aatcccagaa ttccaaacat cgatggaatc atccctttga
     6121 tgaaaggatc tgcgcttcca agcggcccct gatgtaagcc aaaaaagtct ccctgtagaa
     6181 atatcgattc caaaaattgc agtattatga ttggttccat ctcaaagacc tattgcgttt
     6241 attacctcgt ttgcggcctc ttcaaccttt ccttccgagt ttagtattga tttcattgga
     6301 gaccctgtga ttactgcaca gcttgaaagc attgcttctt ggactgcgag ttcttttttt
     6361 atgctatcaa ttgagatgat gtctcgctct cgagtcgtat ccttcattcg ccgattgtag
     6421 atttcttctg gccttgctga gacggtaatg agatttgccg gtttgagttc ttcaaggacg
     6481 tgggacggca aaccaggata gaatccttct tttgttgaga tgaatgcgtg agtgtcaatt
     6541 atgacaatat tgtgattaaa gcttgcgatt ttttttgcgg ccatcttttg tagttctttt
     6601 tgttcttcta cagagagctt tcttagctcg tccctgtttt cgattccgag tttttttgct
     6661 tcctcaaaca tgacggttcc aaaactatga acgcttacgc tttttttctt gctgtttaat
     6721 atctcaacta ttttggatac aagcgtcgtt ttgcctactc cagcaatgcc aactatgaca
     6781 actttcttac tttcgcccaa gcaacgcacc cagacgcggc ataacgactt cgacttgttc
     6841 tctgactaat tgttgatagt agtttatcaa aatgtccacc attagcagta taccaatacc
     6901 gcttccaaac accccaagaa catcagatac gccagccaaa gcaccaagta tggcagaccc
     6961 aattattgtc actgatggaa tgtatttgtt aagaagtgcc tctactggct gatttgattt
     7021 tctaaatcca ggaatttgta cgtctgcatc aagcaagttc tttgcagcgc tctttgggga
     7081 aagacctcca agctctaccc acaaccttcc aaatataatt acgattccta tcataaagaa
     7141 aatatacagt actgccctca tcggatcaag ggctgccaag tccaaccctc tgggcggtgt
     7201 gatatagtaa acaattcctc caattggcgt agagggggcc gtaggatcaa actgcgccaa
     7261 aatattgaag agtggattgt tgtttcttgg attgacgttt gcccataaca tttggcccat
     7321 aaagatggcg tttgcagtaa gtgcagatgc cagaattact ggaatgtttg agacgtacat
     7381 tagcttgatt ggatatgtcg cagaaaatcc cctgtacttt gttgagacga tcggaatctc
     7441 aactttgatt gcctgcgtgt acacaagtag taacatcact cctgccgtaa taaacagccc
     7501 aaatatgctc gggagctgat tagccctgaa aaatatctct gtaatatcac cttgcataat
     7561 tgactgggct acaaacggaa atactcctat gtggctgcca tcgcctgcat aaagcgggct
     7621 gaagatgctc cacaaaattt gttgggcaac tcctgccatg atgaaaagac tgattccgct
     7681 accaagaccc caacctttct ggataagctc gtcaaggaac attatgataa tagatgcagc
     7741 aattagctgc ccaattatga cataaagaac agtcgggttt gcaattcctg gtccatacac
     7801 agctatagca tacactacag actcgataat tattacaata taggtcacaa gctttgttgc
     7861 agtctgaaat actcctcttt cctctggttt tgtaaagtca aacttgagaa tctcagagcc
     7921 ctttagcagt tgcatcaaaa gtcccgctgt aactattggt cctattccga gctcaactag
     7981 agtgccctgc tgagatgcaa agatcaccct tgcaaattgc agaaagtcga attctggagc
     8041 agtagcgcca aaaagtggag tttgtcccat tacttggtag atcaataagg caattccgca
     8101 ccagagtagc cttgtttgta gtgatagctt cttttttggt ttaggtactt ggactaggta
     8161 tggttctgct ttttcaacga ttcttttgac aattcctgtt gcagtgcctt cagccaacag
     8221 ctaacacctc tcccccaacc tttttgatct tttcttctgc agatgccgtg tattgccgaa
     8281 ttttgacaga gtaggcattc tttagctgcc cgcccccaag aagtttatcg tagccaaggc
     8341 tgtccagatc cagaaccttt ttgcctcctt cttgtttgcc gtatttggca aaaaagtcgt
     8401 ctagatctct tacactggcc catttccgtg ttatgtttgg gtgaggtgga tgagttgaat
     8461 catgaccaaa atgatcagga tcgtatttta gcatagagct gaagaggtgt ttgtgcattc
     8521 ctgatttgcc aagacctcct ttgtgaccgc ttgccctgtg ttgaccgatt tggccccaac
     8581 cgtgggttct tgagcctctg agttttcttg tttttcgcag tcttgttgcc aattagatca
     8641 tgttcctgac aagttcgatg agttctttgt tttgcccaag tatgccgcct tctccgtacg
     8701 tttttcgtgt gctgcgtttg aaaccacgtc ttggtggtga tagtgcaaac caaggtttta
     8761 tggggttaat cttatttaga gaaattttgc cttcggtaat ggatgctgca agttcgtcga
     8821 tggttttaaa accaagtttt gtcaggtcat cgtttgtgat ttttttgtag ccggtttttc
     8881 ttgctttttt gtcaaggagc tcttttgtga tatcgaggtt tgcttcttgc catgagacat
     8941 agtgctttac tctatccaac attccaagtg tgttatcttt tgagggaatt attgttgcac
     9001 ggaatttttt ttctagtttt aagagattca gagttgtctt tacccagtgt ggcacgttga
     9061 tttgtcctct cattcttaca acaagatatg cttttggcat cttcaatcaa ccttgactaa
     9121 atgtctgcct caggcattgt agcactgctt ttgatgtaga attcattgtt tcagtagatc
     9181 cttttgctgt agtccatgca tccttgaggc ctgcaagttt gagaagattt ctaattttac
     9241 cgcctgctac aagtccaagc cctcttggtg ctggaacaat ttcaactacg acgcttcctc
     9301 cttttcctcg taccttgaac ggaactgagt gagattggtc gcatctgcac tcccagcttc
     9361 cacaacccag tttgactgga ctcacattaa gataagcgtt agttgttgcc ttttcaattg
     9421 caattctcat ctgtttagat tttccttgtc ctattcctaa ccaaccattt tcatttcctg
     9481 ccgccaccat tgccttgaat tttgttcgtt gtccatttgg cgtcattgtc tgaaccattc
     9541 caacgtcaat tacctcagtt tttagatctg gtaacagttt tttgattatg ccagcttctt
     9601 gaattcttaa tccgttagca tagatttcat ccatagttgt gataagacca gcaaatactt
     9661 ttttgccaag ctctgttcta ggaacccaga cttcctcttc ttttctagat gagcgcctgt
     9721 catgccttgg tctttctgca gctgtttggc tcatttctgc ttgacctcac catcaatgat
     9781 tgacttgaat tttgcaacat cgttttttat ttttagatga tcaccgttaa ttcgattttg
     9841 agatggtaag gcctcctcat ctgctggaat ttgaagtcct gcgtcaatta ctccttttag
     9901 ggcagctgcc attctttgtg tataacgtct tgtaccacta tacaagatta cgtcgttgga
     9961 tcctttggag agtgcctttt ttcctgccag atagccagtc aggtatgcag ctggaacatt
    10021 ttttcgtgat cctttccagc ccttttgtgc aagaaaccta gaatgagctg aagccactac
    10081 tttatcgccg ctcatttgtg gtttatgaat ttgaaccaga gtgttttcat ttgaaatttg
    10141 aacggtgata aaatcacgcc tactcatcag tagtagcttt cgcttcttat agttggtttt
    10201 ttcttcacgt aaacgtcgca gtattgttga atatgccatt gcgatattgc tgaaattttt
    10261 gaactgcttt tataaatgtt cgagagccaa catagaaagt agtgcttttt gtgaatgtag
    10321 acgattttct gcctcatccc aaacaactga ctgcggccca tcaatgatcg aagctgtcac
    10381 ttcttgacct ctttttgctg gcaaacaatg cataaagata gaaccttttt tggacttgga
    10441 cataagtgat gagtttactt ggtattttgg aaagaatttt ccaaatcgct ttggatcatc
    10501 gtgaattgac acaaatgtgt ctgtggccac aatatcggca tcttttactg ccttgacagg
    10561 atcttgtgtt aactcaatat cgatgatctt tttgcaatct tttaccacat tagcaattgg
    10621 ttcaaaccct ttgggagtag caatagacat tgagatacct acttttgagc agccatataa
    10681 catggaatta caaacattat ttccatctcc aacccacgca agctttaatc ctttgagctt
    10741 tcctttcctt tcctttattg tcatcaggtc tgcaagaatt tgacatgggt gaaatgagtt
    10801 tgaaaggccg ttaattactg gaactgtgga atttttagcc aaaacatcaa tggttgaatg
    10861 ttcgtaaact cgtgccatca taatgtccac atatcgtgaa agtgtccttg ccgtatcctc
    10921 aactgtttct ccacgtgaca gctgcaagtc ctgtggcgac agagttaacg catacccccc
    10981 tagctgaaac attcctactt caaaacttac gcgcgttcgc gtagatggtt tttgaaaaat
    11041 cattgcaagg gttttgtttt ttagcaatgg ttttgtgatt ccttttttga gatctttttt
    11101 taattttatt gcaagatcga taatctgcag gacttctttt ttgtccagtt cccctaatgt
    11161 taagacgttt tttactttaa tcatttttta cgccatccga aaagtccctt ctttggcttt
    11221 gcatccttct cttctttaac tttcttttct tcttcacctt ctgacggttc aggttttttc
    11281 ttttcttctt ttgcttgttc gccaagtttt ggacggccgt tttctatcca ttttttaatt
    11341 ttttgagcca aaacaaatgc ctcatcatca ctttctggtg gtggtacatc atacaaatct
    11401 gcataacgcg aaatatcttt gtcattgatt ccttcaaagg gatctttgtt cttttttggt
    11461 tctggtttga gctctggttc tggtttgagc tctggttttg gcaatgtact ctcaccaaat
    11521 actgtttgaa gaaatagttt cttgtcaaat ggtttaagat ccggatattc ttggcccact
    11581 cccacataca aaacaggttt tgaggtaatt ttgactacag atagtgccgc accgccccta
    11641 gcatcagcat cgctttttgt tagaattgca ccatcaaact ccacatgctg ataaaattcc
    11701 cttgcctgac tcacggtgtc gttgccagca agcgagtcgc ctacgaaaat ctttagatct
    11761 ggctttacta cctttgttat cttttcaatt tgctccatta ggtttttgct tgtttgcatt
    11821 cttccggcag tatcgattag cacgcagtca attttgtggg attttgcgta aagtactgca
    11881 tctcgtgcta ctgcagcagg atctgcccca tagttttgtg caattatctt tagattcaat
    11941 ctgtcagcat gctcttttag ttgctctatt gcgccagccc taaacgtatc agccgcagcg
    12001 atcgcaatgg aaaatttgtt ttcttttaac aaataagcaa gcttggcaag tgaggtggtt
    12061 ttgccagtcc cattaattcc tacaaataaa atgataaacg gttcgctaga actttttttt
    12121 gagttaattt ttgatagtat gtctatggtt cccgcagtat caaacaagtt agaaatattt
    12181 tgaatcaaat tatttttaac aaattcttca atttcttttt tttcaataga tgttccaaca
    12241 agtttcttct taagatcaga ttttatagaa tctattactt ctgttgcaac gtcagattcc
    12301 atcagtgcaa tttctaattg aaaaagaacg tcatcaatgt ctccttcctt tagctccctc
    12361 tcactcagac ttttagcaac cgatgagaat gcggt
//
LOCUS       DASWKH010000134         4896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2561079, whole genome shotgun sequence.
ACCESSION   DASWKH010000134 DASWKH010000000
VERSION     DASWKH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561079"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..586
                     /locus_tag="VGA92_09530"
     CDS             218..586
                     /locus_tag="VGA92_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846681.1"
                     /translation="MEIVIEPWKKLIIHEIIEYNFDDWVTQIAFGSKTAGGAIPTINW
                     VNGVVFQSFNFPDTNAIVEEKLKGILHWSSVMFATKERYEKQIIKDNATINLIDVSVN
                     EVFRVLAEKLKEHSRQKSRP"
     gene            700..1599
                     /locus_tag="VGA92_09535"
     CDS             700..1599
                     /locus_tag="VGA92_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEX9846682.1"
                     /translation="MTSSQPKCPSCAKTTMQTDDATGEMFCRNCGFVAAEKLEESGPE
                     WRSFSNDESDRSRVGAGTSLTMHDMGLSTVIGAADKDATGKPLSASMKSSIMRLRTWD
                     SRTQAHTSADRNLRQALNELNKMKDKMGLADSVIEKAAYIYRKAMEKKLVRGRSIHGL
                     VAACLYAACRNTETPRTLDDVAESINIRRKDVARCYRLIYKELDLKMPVADPTKGVSR
                     IASMANLSEKTKRKATEILNKAKKIGMAAGKDPMGLAAAALYLACISNGEIRSQKDIS
                     VAAGVTEVTIRNRCVGLKGLLEK"
     gene            1649..1936
                     /locus_tag="VGA92_09540"
     CDS             1649..1936
                     /locus_tag="VGA92_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846683.1"
                     /translation="MSDAISWSDWLDFSEEQVAKVPESTGVYMMHAAMKILIIGNSTN
                     LKQSIAESLKTPCTSDAKRFRYFVTDLHLEIGQKLLKEYQEKHDGNLPKCM"
     gene            complement(1937..2176)
                     /locus_tag="VGA92_09545"
     CDS             complement(1937..2176)
                     /locus_tag="VGA92_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9846684.1"
                     /translation="MATAYILINCDMGSEDSVITQLKSIDDVREVHGTFGAYDVLAKI
                     SSEKAENIRDTITWRIRKINQIRSTLTLMGIEGQE"
     gene            complement(2486..3085)
                     /locus_tag="VGA92_09550"
     CDS             complement(2486..3085)
                     /locus_tag="VGA92_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846685.1"
                     /translation="MQIFVLKPVPHLFMASLSDIFHNGKQMRKKSHEVTKLSENKIAP
                     KFVIGNPEAFTKNRETLGNIIVKNMNASGGMDVDWLVERKGNFIILEIKSFYDDRIRI
                     PLGQIIAYQSLHEKLNENGKCYLYVIGVDENDFTDDCSKIWLFEMKEWREQKIPHISR
                     NFEESDSPKDGFIVERSFMREMTVHEFRDLVESAWKEFN"
     gene            3180..3878
                     /locus_tag="VGA92_09555"
     CDS             3180..3878
                     /locus_tag="VGA92_09555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9846686.1"
                     /translation="MSDEKKHQKDASLKFDKLIHIGELTSRITHDMRNPLTVIINYSM
                     MVRKNSRGKLDKKSLDQLSLIEEEARKMYHQIEDVLNYVKLPPLKLESYSLHDILKKV
                     IERVQISDDVEIHLPKNNPRIVCDIDKMEIVFVNLITNAVEAMNGSGTITISAKNEQS
                     DIVIEFEDSGPGISDENLDKVFEPLFTTKENGTGLGLASCKNIIERHNGTISVKNDPT
                     RFTIQLPKIDSQIR"
     gene            complement(3871..>4896)
                     /locus_tag="VGA92_09560"
     CDS             complement(3871..>4896)
                     /locus_tag="VGA92_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9846687.1"
                     /translation="LVSAEVWVPEDEFHGYFDSNGVYTVVGAVRNTEDFSVLPTIEIK
                     INEDGSQVSAIQQIPTVFPHRDIPFKLKFPEIKSQNIVLEKPSVSFEKIIKPASSVQI
                     IYDKTLVKHNDGHLTGRIMNQGNTTEFDLKVYATIHGDNNKFLDVGKNAEKIDKIEAG
                     QILEFTIYPDPLFASEVNYYSCFAIGDETIVPLYAIRDGEKFNFRYDSTAAFTVVGFD
                     ELGTTLSIDGINSFKVPTYVNFEFPRTSDGEQFNVLVNDRQAEFIQSKDEEGNWHVAF
                     GVDGASQSKIVISGFEKPQTTSLLDIKSKTDEKTDYAFLYYAAAIIAVVVGIFLYKYK
                     ARKKLTT"
BASE COUNT         1579 a          985 c          947 g         1385 t
ORIGIN      
        1 ccccatgtat actttggaat caatttcttc tagaccaaaa aaagctaaag cctcaacgat
       61 ttctttttgg ttcatcagaa tccccaaatt accatatctg aacaaaataa tagatattgt
      121 ttgttcaaaa tagacagaca attgcctaat accacatttg cgatcacctc tgattcgttg
      181 aaaattttat aatcatggcg ctttgcaaaa tataagtttg gaaatagtaa ttgaaccttg
      241 gaaaaagctg ataatacacg aaataatcga gtacaacttt gatgactggg tgactcagat
      301 tgcctttgga agcaagactg ccggcggtgc aatcccgacc attaactggg tcaacggagt
      361 tgtttttcaa tcatttaatt ttcccgacac gaacgcaata gttgaggaaa agctaaaagg
      421 aatattgcat tggtcctcag tgatgtttgc aaccaaggaa aggtatgaaa aacaaatcat
      481 aaaggacaac gcaacaatca atctcattga tgtgagtgtc aatgaggttt tcagagtgct
      541 tgcagaaaag ctaaaagagc attcaagaca aaaatcacgt ccgtagattc caagcctgtt
      601 ccatacccat agaatggtga ttagcttaac ctaccaacaa ataaccatag aaattatgca
      661 atactaataa taagtgaaaa gtgcatatat tatacgcaaa tgacatcatc acaacccaaa
      721 tgtccatcat gtgcaaaaac cacaatgcag actgacgacg ccacaggcga gatgttttgc
      781 aggaattgtg gctttgtcgc agctgaaaag ctagaagaat caggaccaga gtggagatca
      841 ttttcaaatg atgaatccga caggagcagg gtaggtgcag ggacatccct cacaatgcac
      901 gacatggggc tctcaactgt aatcggtgcg gcagacaagg atgccactgg aaagccgctt
      961 tcagcatcga tgaagagctc aattatgcga ctcagaacat gggacagtag aacgcaggca
     1021 cacacatctg ctgacagaaa cctaagacag gcgttaaatg agcttaacaa aatgaaggac
     1081 aagatgggat tagcagactc ggtcatagaa aaggcagcat acatctacag aaaggccatg
     1141 gaaaaaaaac tggtaagggg aagatcgatt catggcctag tagcagcatg tctgtatgcc
     1201 gcatgcagaa acacagagac accaagaacc ctagatgatg tagcagaaag catcaacatt
     1261 agacgaaaag atgtggcgcg ttgctacaga ctaatctaca aagagctaga tctcaagatg
     1321 cctgttgctg atccaacaaa gggagtatcc aggattgcaa gcatggcaaa cctaagtgaa
     1381 aaaacaaaga gaaaggcaac ggagatccta aacaaagcaa aaaagatagg aatggctgca
     1441 ggaaaagacc cgatgggact tgcagctgca gccctttact tggcgtgcat ctcaaatggc
     1501 gaaattcgct cacaaaaaga catttctgtt gctgcaggag taaccgaagt caccatcaga
     1561 aacagatgcg taggactcaa aggcctactt gaaaaataac cttccctttt tgttaaaaat
     1621 ttaaggcggc acgctacaac taaaaaacat gtcagatgcg atcagctggt cagattggct
     1681 tgattttagt gaagaacagg tagcaaaggt gccagaatct actggagtct acatgatgca
     1741 tgctgcaatg aagatattga ttataggaaa ttcaaccaat ctcaaacaaa gcattgccga
     1801 atcactaaaa actccatgca cgtcggatgc aaagagattc cgctactttg tgaccgattt
     1861 acacctagaa attggccaaa aactgctaaa agaataccaa gaaaaacacg atggaaacct
     1921 cccaaagtgc atgtagttat tcttggcctt cgatacccat caatgttagt gtagaacgaa
     1981 tctggttgat ttttcgaatt ctccacgtta ttgtatcacg tatgttttca gccttttctg
     2041 atgagatctt ggctagaaca tcataggcgc caaatgttcc atgcacctcc cttacgtcat
     2101 caatggattt tagctgtgtg atcaccgagt cttcagagcc catatcgcag ttgattagaa
     2161 tgtatgctgt tgccatatct ccgatacgta tctaacatat ttattataaa ttatagaaaa
     2221 gatgaacatc tgataagatt tattttataa ttgtggatat tcgcaagata acaatcagat
     2281 aaacagaaca taactaaatg aatgttagtt tgaatgccca aagtggtttt ttccaatcgc
     2341 ttcgttgttt gtgtcatgca gacacacaca agaatagtaa ctaacatctt ttttagtaga
     2401 aataggtctg ttggaccagt gccataattt tctcccttgc cagaccaatg gtaacccagg
     2461 tcctacagaa ccgattttca taaaactagt tgaattcctt ccaagcagat tctactaggt
     2521 ccctaaactc gtgtacagtc atttcgcgca taaatgatct ttctacaata aagccatctt
     2581 ttggcgagtc gctttcctcg aaatttcgtg atatgtgcgg gattttttgt tcacgccatt
     2641 ctttcatttc aaacagccat atcttgctgc agtcatcggt aaagtcgttc tcatcaactc
     2701 caattacata cagatagcac ttgccgtttt cgtttaactt ttcatgtagg ctttggtagg
     2761 cgatgatttg gcctagggga attctaattc tatcatcgta gaaacttttg atttcaagaa
     2821 tgatgaagtt gccttttctt tccaccaacc aatcaacatc catgcctcca gacgcattca
     2881 tgtttttgac gataatattg ccaagtgttt cgcgattttt agtaaaagcc tctgggtttc
     2941 ctattacaaa ttttggcgcg attttattct cagacagctt tgttacttcg tgtgactttt
     3001 ttctcatttg tttcccatta tggaagatat cgcttaggct tgccatgaat agatgtggta
     3061 ctggctttag aacaaagatt tgcatgtaaa gttttgtaca aaaagctgaa tgatacctaa
     3121 cttattttga catagcacaa gataaaataa tatgagcacc acatcccagc ctatctgaga
     3181 tgtctgacga aaaaaaacac caaaaagatg ccagtctgaa attcgacaaa ctaatacaca
     3241 ttggagagct gacctctcgc attacgcatg atatgcgaaa cccacttacg gtaattatca
     3301 attattctat gatggttaga aaaaactcca gaggcaagct cgataaaaaa tcacttgatc
     3361 aactatcatt aattgaagaa gaggcacgaa agatgtacca ccaaattgaa gacgtattaa
     3421 attacgtaaa actcccgccc ctaaaacttg agagttattc gctgcacgac attttgaaaa
     3481 aagttatcga acgagtacag atttcagatg atgttgagat ccatttacca aaaaataatc
     3541 caaggattgt ttgcgatatt gacaagatgg aaattgtttt tgtgaatttg atcacaaacg
     3601 cagtggaagc gatgaatggc agtggtacga tcaccatttc tgccaaaaac gagcaaagtg
     3661 acatagttat tgaatttgag gactcgggtc cgggcatatc tgatgaaaat cttgacaaag
     3721 tgtttgagcc attatttaca acaaaagaaa atggaactgg tttgggcctt gcaagctgta
     3781 aaaacatcat tgagcgccac aatggcacca tttctgtaaa aaatgatcca actagattta
     3841 caatacaact tccaaaaata gattcccaga tcaggtagtt agtttttttc tggctttgta
     3901 cttgtacaaa aatattccca ccacaactgc aataattgcg gcagcatagt acaaaaaggc
     3961 ataatctgtt ttctcatctg ttttgctttt aatatctagc aaagatgttg tttgtggttt
     4021 ttcaaagcca ctgattacaa ttttgctctg agatgcccca tccacaccaa atgccacatg
     4081 ccagtttcct tcttcgtcct tgctttgaat gaattcggct tgcctgtcat ttacaaggac
     4141 gttgaattgt tcgccgtccg aagttcttgg aaattcaaaa tttacatagg ttggaacctt
     4201 aaaggagtta atcccatcaa tggacagtgt tgttcccaat tcatcaaaac caacaactgt
     4261 aaatgccgcc gtagaatcgt atctaaagtt gaacttttcc ccatcccgga tggcatacag
     4321 tggcactatg gtttcatcgc caattgcaaa acaactataa taattaactt cggacgcaaa
     4381 caacggatcc ggataaatcg taaactccaa gatttgcccg gcttcaattt tgtctatttt
     4441 ttcagcattt tttccaacat caaggaattt gttgttgtca ccatgaattg tcgcgtagac
     4501 tttgagatcg aattctgtgg tgttgccctg attcattatt ctaccagtca ggtgtccgtc
     4561 attgtgtttc actagtgttt tgtcgtagat tatttgaacg cttgatgcgg gtttgataat
     4621 tttttcaaat gacacgcttg gtttttctag aacaatgttc tggcttttaa tttcaggaaa
     4681 cttgagcttg aatggaatgt ctctgtgtgg aaatactgtt ggaatctgct gaatcgcgga
     4741 gacttggctg ccatcttcat tgatttttat ttctattgta ggtaaaacag aaaaatcttc
     4801 agtgtttctt actgccccaa caacagtata gacaccattt gaatcaaaat aaccatggaa
     4861 ttcatcttct ggaacccaaa cctcagctga gaccaa
//
LOCUS       DASWKH010000135         6843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_1453617, whole genome shotgun sequence.
ACCESSION   DASWKH010000135 DASWKH010000000
VERSION     DASWKH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453617"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGA92_09565"
     CDS             complement(<1..215)
                     /locus_tag="VGA92_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEX9846688.1"
                     /translation="MMAQLGQKAPNLSVSKWVQGMPTNIDQEKDRIVLVEVFQVNCPG
                     CFLYGIPEAINIYDKYHKDGVRVLGLAT"
     gene            306..794
                     /locus_tag="VGA92_09570"
     CDS             306..794
                     /locus_tag="VGA92_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX9846689.1"
                     /translation="MSAVPEQRLALFAEMEDRYEKKDTGYFVKLLDNEDYVIRTRATC
                     ILVDFGGEDKVPFVAKVLKDDPNELVRHEAAFSLGQMGYRSAIKHLEEATKNDPSMFV
                     RHEAAIALGVVGAKEAKQTLEDALKDPSVPVVESAIVALSNIQFMEKLSKNEKFAKLT
                     GG"
     gene            complement(902..2986)
                     /locus_tag="VGA92_09575"
     CDS             complement(902..2986)
                     /locus_tag="VGA92_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEX9846690.1"
                     /translation="MIKSVELGNFISHAETKLDFEDGVTVFVGHNGAGKSSVIDAITF
                     ALFGEHTRKSNKGLIRRGTSQAYAKINFSSNGRNYEAVRKIDSKGTLSAQFTEIRDGQ
                     QIQLATGERKQFGESMTEQIESRLGLDFTKLKVASIVQQGELNAIIKAKPKEFKELLN
                     AIIGIDKLDLASESLKAVQKNFRHLVQQKIGYDDTHSDILQKELEQKQQEIKRAEPQK
                     QELQKRKQAQEKELQELELKIKADSQKESILEQLEERRQELHRYVREAIIGIQKEISE
                     KERKMAECEGCFECARQCPDLESMLQNAESEISEIDKKILGFEKQQTILQEHQILAKK
                     LQLQDGRCPVCDTNVEHLKPIFQVEHIRSEQEKTRQELDVLASKKQQAIKDKNEVSVK
                     LKEAILAQRTLDAHAIQRPEQLQQIKNEISLQKAQIQKVPAMNSSGLLIEAASIDSHA
                     RLLCEKIESLQKEISGFDPTEFSRLKVEFERKRQDASKIDQEYGAVTEKIKQASHRIE
                     KISTILFELGHVKEYLVHLGEIQSNVYNRDGPVATSLRSWALATISEKASEYLEMLNT
                     KIHRITLAEKTRDVIITCYSKNTVIDIDSLSGGEQVSVALALRLGMAHLLGASNLNFV
                     ILDEPTTHLDEERRRSLVRVLSQLSDISSNVSGPLQLIIITHDSEIFEDSTVEKIYKF
                     EASPDGTKVSLL"
     gene            complement(2983..4122)
                     /locus_tag="VGA92_09580"
     CDS             complement(2983..4122)
                     /locus_tag="VGA92_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9846691.1"
                     /translation="MLFSHISDTHLGLTQYGLEERENDIYSAFNEAIDTSVRDHVDFV
                     IFAGDIFHVPNPSGTAIIQMANALRRLKENDIDSFFVLGEHDISRIRSTPIPYVYHNL
                     KFSKYIGDGAPFVYKDVFLAGFDKMRKNEVVRFAEKFAELDMAAQRHNGHKILVMHQG
                     ISEINQFASELSSADLPRNFTYYAMGHLHDRVEKRFSRLGGPLVYPGSIEMTTSEGIK
                     ETQKGFYEVDISAPEPHPSWIKLDTRPQLSVKTEFENLADAISNTLEKITKYTKKPIV
                     ELKISGNEIKVEAVQGHLAHLAPHTLHCTWKIIHRGGDGSSVLAERPSSIDDELFRLA
                     TNYLGSEKIANFAIRELLPVLASGKIDEAGQMIAENFEQFRVTKK"
     gene            complement(4125..5651)
                     /locus_tag="VGA92_09585"
     CDS             complement(4125..5651)
                     /locus_tag="VGA92_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9846692.1"
                     /translation="MTLGVVIGESKPTSVTAQTSRPLSVGEYVIVDSQDGKLLGLVER
                     SFVQSAALTDIHNFDEAIESKEIADINSRDKSFTAKITILGYLSELQKSRVVLPAIPP
                     IPGTSVLDATKDDLGMIFAPQKDEWAKVGTLLRNRQIEACVNLNKVVSRHLGILAMTG
                     MGKSNLVSLLAKQIATLDGTLIIFDYHNDYSELHIPRINIIDAKINPRLLDANTLGDV
                     LEIRENADVQQRILRLAFTPDIKEAKDFWDALDHSVAMIGANPERKDDRHSADRVQDK
                     IDDARHRFADILDPDIMDPLGLIKEGRVNILNISELSEKQANVALAYYFQELLNDRKA
                     ALAAKNAKNKAAKRYRFDSPIFVIVEEAHVFIPKGEETHTKYWAAKIAREGRKFGLGL
                     GIVSQRPRNIDPNVLSQMGSLAVMKIVQEDDQHQIASAAESISRDLINQLTSLNVGDA
                     VFVGQWVNLPAMVHIEEVKDKVVGSDKNAVAEWRHAKKFEKIAAESTQGLVQKDLLLD
                     "
     gene            5999..6808
                     /locus_tag="VGA92_09590"
     CDS             5999..6808
                     /locus_tag="VGA92_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846693.1"
                     /translation="MCSYATLNYPDTILAKAVEEKVAKKLHKHFSSLGLDCKLIPDGT
                     MDFEYDYPRASLEGPTPGIASRGVMKVNDSIDYVDMLKRKTITKSEFAPGGIHGMGIA
                     EGITWKLRFFISFPSEVSLGPLNIGTLTKILKGRLHSKVDDFIWDGYGKLTTLPPGMI
                     PDDVIATLNADKRLRELMMHSLLKERVITISVYKPKTKVNYDYLQIVKDDSYWYKYKP
                     KKESYARIVISSEWRGQKDIFIDKETLEMYQRIAANIRTMVEKLEYHLIKK"
BASE COUNT         1768 a         1482 c         1442 g         2151 t
ORIGIN      
        1 gtagctaacc caagtactcg caccccatct ttgtggtact tgtcgtagat gtttattgcc
       61 tctgggattc catacagaaa gcagccgggg cagtttacct gaaaaacttc tactaggaca
      121 atgcgatctt tttcctgatc gatgttggtg ggcattccct gcacccactt tgatactgag
      181 aggtttggtg ccttttgtcc taactgcgcc atcaataatt ttgattgttt tttgtttatt
      241 ataactgtaa gtatggtttt tgcgaattag agtaatatag tgcagaactt ttctgcaaat
      301 cagaaatgag tgcagtacct gagcaaagac tagctttgtt tgcagaaatg gaggacagat
      361 atgaaaaaaa ggacactggt tattttgtca agctcctaga caatgaggat tatgtgataa
      421 ggacaagggc aacatgcatc ctagttgact ttggtggtga agacaaggtg ccatttgttg
      481 ccaaggttct aaaagacgac ccaaacgagc ttgtgcgaca cgaggcggca ttctcgcttg
      541 gccagatggg atataggagt gcaataaaac acctagaaga agcaacaaaa aacgatccaa
      601 gcatgtttgt taggcatgaa gcggcaattg ctctaggcgt agttggggca aaggaggcaa
      661 aacagacact tgaagatgcg ctaaaagacc caagtgttcc agtagtagag tcagcaattg
      721 ttgcactatc gaacatccag ttcatggaaa agctcagcaa gaatgaaaaa tttgccaagc
      781 ttactggcgg ctagcaccgt aatcttttgt ggctgtgtgt ttatccacga tctattacaa
      841 aaatattgaa ttcgccttaa aaatggtgga taataaaatc atcaaatttg tttgaatgct
      901 tctaaagaag tgaaaccttg gttccatcag gactggcctc aaacttgtag attttctcaa
      961 cagtcgagtc ctcaaagatt tcagaatcgt gtgtaatgat gattagctgc aacggtcctg
     1021 atacattact ggagatgtct gagagctggg acaaaactct gactagggat ctccgccttt
     1081 cttcgtccaa atgtgtcgtt ggctcatcaa gtatcacaaa gttgaggttt gatgccccaa
     1141 gcaggtgtgc catcccgagc ctgagggcta atgcaacgct tacctgctcg ccacctgaga
     1201 gagagtcgat gtcaattacc gtatttttag agtaacaagt aatgatgaca tcacgcgttt
     1261 tttctgcaag tgtgattctg tgaattttcg tgttcagcat ttcaagatat tccgatgcct
     1321 tctctgatat ggttgccagc gcccacgacc taagacttgt ggcaactgga ccatctcgat
     1381 tgtaaacatt actttgaatc tcgcctagat gtacaagata ctctttgaca tgccctagct
     1441 caaaaagaat ggtgctgatt ttttcaattc tgtggcttgc ctgtttgatc ttttctgtga
     1501 ctgccccata ctcctgatca attttgcttg catcctgtcg ttttctctca aattctacct
     1561 ttagtctact gaattccgtt gggtcaaagc cagaaatttc cttttgaagg gattctattt
     1621 tttcacaaag gagccttgca tgcgagtcga ttgaggctgc ctcaattaaa agccctgagg
     1681 aattcatggc cggcaccttt tggatttggg ctttctggag gctgatttca ttttttattt
     1741 gctggagttg ctcaggcctc tgtattgcgt gggcatcaag ggttctttgg gctaaaatcg
     1801 cctctttgag cttaacggac acctcgtttt tgtctttgat ggcttgttgc ttttttgatg
     1861 ctagcacatc aagctcttgc cttgtttttt cctgctctga tctaatgtgc tcgacttgga
     1921 agatgggctt gaggtgttct acattggtat cacaaacggg gcatctgccg tcttgaagct
     1981 gcagtttttt tgcaagaatc tggtgttcct gtaaaatggt ttgttgtttt tcaaaaccca
     2041 atatcttttt gtcaatttct gatatttcag attctgcatt ttgcaacatt gattcaagat
     2101 ctggacactg tcttgcgcat tcaaaacaac cttcacactc tgccatcttg cgttcttttt
     2161 cagaaatttc cttttgaatt cctatgattg cctctcttac atatctgtgc agctcttgcc
     2221 ttctttcctc aagctgttct agtattgact ctttttgcga gtctgctttt atttttagtt
     2281 ccagctcttg cagttctttt tcttgggcct gttttctttt ttgcagctct tgtttttgtg
     2341 gctctgctct tttgatctct tgttgttttt gctctaattc tttttgcaga atgtctgagt
     2401 gtgtatcatc ataaccaatc ttttgctgaa ctaaatgcct aaagtttttt tgtactgcct
     2461 tgagggattc ggatgccaga tcaagcttgt cgattccaat tatcgcgttg agtagttcct
     2521 tgaactcttt tggctttgcc ttaattatcg cgttgagctc tccttgctga acaattgatg
     2581 cgacttttag ctttgtaaag tcaagaccca gtcttgattc tatttgttca gtcatggact
     2641 cgccaaactg ttttcgctct ccagttgcaa gttgtatctg ctgaccgtcc cttatctcag
     2701 tgaactgcgc agaaagtgtt ccctttgagt caatttttct tacggcttca taatttctcc
     2761 cgtttgatga aaaattaatc ttggcgtacg cctggctcgt tccgcgcctg attaagccct
     2821 tatttgactt tcttgtatgt tccccaaaca atgcaaacgt tattgcatca atgacacttg
     2881 attttcctgc tccgttgtga ccaacaaaaa cagttacgcc gtcttcaaag tccagctttg
     2941 tttctgcatg ggaaatgaaa ttcccaagct caactgattt tatcatttct ttgtcaccct
     3001 gaactgctca aagttttccg caatcatctg gcctgcctcg tcgattttgc ctgatgcgag
     3061 cactggaagt agctccctta ttgcaaagtt ggcaatcttt tctgagccga gatagtttgt
     3121 cgcaagcctg aataactcat catcaatact cgaaggcctc tcagctagga ccgacgatcc
     3181 gtcaccacct ctatggatta tcttccatgt gcagtgtagt gtgtgggggg caagatgagc
     3241 aagatgtccc tgcactgcct ctactttgat ctcatttcca gaaattttca gctcgacaat
     3301 tggctttttt gtatattttg taatcttttc tagtgtgttg ctaatggcat ctgcaagatt
     3361 ttcaaattcc gtttttactg aaagctgcgg ccttgtatca agcttaatcc agcttgggtg
     3421 tggctctggt gcagaaatgt ctacttcgta gaatcccttc tgtgtctcct ttattccctc
     3481 actagtggtc atttcaattg agccagggta cacaagcggt ccaccaagac gtgagaagcg
     3541 tttttctact ctgtcgtgta ggtggcccat cgcataatag gtaaagtttc ttggcaggtc
     3601 agctgagctg agctcagacg caaactggtt tatctcagaa atcccttggt gcatgactag
     3661 tattttgtgg ccattgtgtc tttgtgccgc catatcaagt tcagcaaatt tttcagcaaa
     3721 tcttacaacc tcattttttc gcatcttatc aaaccccgcc aaaaaaacgt ccttgtaaac
     3781 aaacggcgca ccgtcgccaa tgtattttga aaatttgagg ttgtgataca catacggaat
     3841 tggggttgag cgaattctgg atatgtcgtg ttcgcctagg acaaaaaatg aatcaatgtc
     3901 gttttccttt aggcgcctaa gtgcatttgc catctggatg attgcagtgc cgcttgggtt
     3961 tgggacatgg aaaatgtctc ctgcaaaaat aacaaaatca acatgatccc taactgatgt
     4021 gtcgattgcc tcgttaaacg cgctgtaaat gtcgttttct ctttcctcta ggccgtactg
     4081 tgtgagccca agatgtgtat ctgaaatgtg ggaaaataac atcttcaatc caaaagtagg
     4141 tctttttgaa ctagtccttg cgtagattcc gctgcgattt tttcaaattt ttttgcgtga
     4201 cgccattcgg ctacggcatt tttgtcagag ccaaccacct tgtcttttac ctcctcgatg
     4261 tgaaccattg caggcagatt gacccactgc ccaacaaaca ctgcgtctcc gacattaagt
     4321 gatgtgagtt ggtttatcaa atccctgcta attgattcgg cagcagacgc aatctggtgc
     4381 tggtcatctt cttggactat cttcattact gcaagtgagc ccatctggga gaggacgttt
     4441 gggtctatgt ttcttggcct ctgcgataca atgcctaggc ccaagccgaa ctttcttccc
     4501 tctcgagcaa tttttgcggc ccaatatttt gtgtgggttt cttcgccctt tggaatgaat
     4561 acgtgcgctt cctcaacaat tacaaaaatt ggcgagtcaa agcggtatcg ttttgccgct
     4621 ttgttttttg cattttttgc tgcaagtgca gcctttctgt cgtttaacag ctcttgaaaa
     4681 taataagcaa gtgcgacgtt tgcttgcttt tcactcagct cagatatgtt gagtatgttc
     4741 acccttccct ctttgatcag tccgagggga tccatgatgt caggatccaa aatgtctgca
     4801 aagcggtgtc ttgcatcgtc aattttgtcc tggaccctgt ctgcagaatg tctgtcatcc
     4861 tttcgttctg ggtttgcgcc aatcattgca acgctgtggt caagcgcatc ccaaaaatcc
     4921 ttggcctctt ttatgtccgg cgtaaacgca aggcgcagga ttcgctgctg gacgtcagcg
     4981 ttttcgcgaa tctccaaaac atcgccaagt gtgtttgcat ccaaaagcct tggatttatc
     5041 tttgcatcaa ttatgttgat tctgggaatg tgcagctcag agtaatcatt atggtaatca
     5101 aaaatgatca gcgtgccatc aagtgttgca atctgttttg ccaaaagcga gactaggttg
     5161 ctttttccca ttccagtcat cgccaaaatt ccaagatggc gagagacgac cttgttgaga
     5221 ttcacacacg cctcaatttg ccgatttctt agcagcgtgc caactttggc ccactcgtct
     5281 ttttgtggtg cgaatatcat gccaagatca tcttttgtcg catccagaac ggatgtgccg
     5341 ggaattggcg gaattgccgg aaggacaact cggctttttt gcagctctga cagatagccc
     5401 aaaattgtaa tttttgcagt aaaacttttg tcgcgtgaat taatgtctgc aatttccttg
     5461 ctttctattg cctcatcaaa gttgtggatg tcggttaggg cggcactctg gacaaaagag
     5521 cgctcaacta atccaagaag ctttccatct tgtgaatcaa ctatcacata ttccccgact
     5581 gaaaggggcc ttgatgtttg cgcggttacg gaagtcggct ttgattcccc aattacaaca
     5641 cctaatgtca ttttttaatc acactaaact atgtacatcg ccttaagaga ttttcctgcc
     5701 actgaaatat gaaaaaaact gtcacaaaaa atcctagaga tttccagtaa ttaaattggc
     5761 agattagctt ttagctaagc tgtactgcgt gtgctttgat cgaaaccatt catttgtcag
     5821 taagatatca aatcgatttt tgaaactgac agttgggtat cttgtagatc aattaaaaac
     5881 caaaaagaca cgtgtagcta aaaaatatgt ttttttcaat aaataatgta aaatatgcaa
     5941 aaaaattcaa aaattataaa attattcttt tattgataga tactcttaaa aatattaggt
     6001 gtgtagctat gctacgttaa attatcctga tacgattctg gcaaaggcag tagaagaaaa
     6061 ggtggccaag aagctgcaca agcacttctc gtcacttggg ctagactgca agctaattcc
     6121 agacggcacg atggactttg agtatgacta tccacgagcc tcccttgaag gtccaactcc
     6181 tggaatcgca agcaggggtg taatgaaggt aaatgacagt attgactatg tggatatgct
     6241 aaaaagaaag accataacaa aaagcgaatt tgccccaggg ggcatacatg gaatgggcat
     6301 agccgaagga atcacgtgga agctaaggtt tttcatatca ttcccatccg aagtcagcct
     6361 tgggccactt aacattggca ccttgactaa gatcctaaaa ggcaggctac attccaaagt
     6421 ggatgatttc atctgggatg gatacggcaa gcttacaacg cttccgccag gaatgattcc
     6481 agacgatgtc attgccacac taaatgccga caaacggtta cgcgagctaa tgatgcactc
     6541 tttgttaaag gagcgagtaa tcacaatatc cgtctacaaa ccaaagacaa aggtaaacta
     6601 tgactatctg cagatagtaa aggacgactc gtattggtac aagtacaagc caaaaaagga
     6661 gtcatacgca agaatcgtca tcagctcaga gtggagggga caaaaagaca tcttcataga
     6721 caaagagaca ctagagatgt accaaagaat tgctgcaaac atcagaacca tggtggaaaa
     6781 actagagtac cacctaatca aaaaataaat atcttcgaaa actattccgc ttcttttaga
     6841 atc
//
LOCUS       DASWKH010000136        25574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3307076, whole genome shotgun sequence.
ACCESSION   DASWKH010000136 DASWKH010000000
VERSION     DASWKH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 25574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25574
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307076"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VGA92_09595"
     CDS             <1..431
                     /locus_tag="VGA92_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HEX9846694.1"
                     /translation="NIASQVERMLETTTDIVYFVTTLEDISRLYHSEIPEKIKLAEKA
                     GGTVRLILDEVSPKMLPFVKRFGASEVRLGKLPSKGRIVVSKNCPITGKLLMSDSAVR
                     GTLHENIDAECALFTNSEEMTNNIFTLCTFIWKGADVIKL"
     gene            474..1148
                     /gene="tpiA"
                     /locus_tag="VGA92_09600"
     CDS             474..1148
                     /gene="tpiA"
                     /locus_tag="VGA92_09600"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9846695.1"
                     /translation="MFIINYKNYEEISGDKAAKLAAVASSISRKYKVKIAIAPPHHLL
                     SAVKNYSGPILAQHVDNARVGSTTGFVIPELLKKSKISGSLINHSEHRIPSKDIVELV
                     NRLRNLKMISVVCVKDVPEAAKYAKLNPTFIAIEPPELIGTGRAISTERPELITKAVQ
                     AVKAAKNKTKLLCGAGIVTGKDIRKAIELGSEGILVASGIVKAKNWNKIVEEFAKGML
                     KISSNR"
     gene            1168..3843
                     /gene="ppdK"
                     /locus_tag="VGA92_09605"
     CDS             1168..3843
                     /gene="ppdK"
                     /locus_tag="VGA92_09605"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9846696.1"
                     /translation="MKMVYFFDEGDGKNKKLLGGKGAGLCEMTRLRLPVPPGFTITTE
                     VCKTYYQNNKTLPKSLWSEVKKNIAKLERRTGKKWNSNQNPLLVSVRSGAALSMPGMM
                     DTILNLGLNDSAVESLTKKTNNPRFAWDSYRRFVQLFGKVVFGVNDKKFDEVLDGAKK
                     KQGIHEDSHLSEQSLREVVAQYKQICQDHTGKPFPSHADEQLQLAIRAVFNSWMGERA
                     IVYREKNHITKDIADGTAVNVVAMAFGNMGDDSATGVVFTRSPADGTNKIFGDYLVNA
                     QGEDVVAGVRTPKPVDEMKKELPKGYKLLLDTCKKLERHYKEPQDIEFTIEQGKFYLL
                     QTRSAKMNAVGMIKTSVDMVKEKLITKDRALLRLQPDQLEQMLHKTIDYEKARQHKKV
                     TKGIAASPGAASGVAVFDVKRAVAMGENGAKVILVREETKPEDVPAFFTSVAILTSRG
                     GKTSHAAVVARGMGKPCIVGCSDLKINADAKQATTGDVVIHEGDAITIDGSIGEVYLG
                     EVPTIEPQITDDFRTILSWAQKAKKIGIRANADTPDGAQLARKYGAQGIGLCRTERMF
                     NAKERLGLFVNLIMAKTLEERKLHLQKLQELQKSDFVQILKAMEGYKVTIRLLDPPLH
                     EFLPNPEELINKIHRLEKQGSSQELEEVKILLARAKELAEVNPMMGHRGVRVGVTYPE
                     IYEMQIRAVFEAAAELAKQKVHAHPQIMIPQVGSVAELNHIKQIYNKVKSEIESKHRM
                     RLPINFGTMLEVVRGCLTSGELAQTAEFFSFGTNDLTQAVFSFSREDAEGKFLPEYLE
                     KEILERNPFQTVDVNGVGSLVKIAIANGKAVKPSLEIGVCGEHGGDPNSIKFFHSVGV
                     SYVSASPHRIPIAIVAAAQANLIAKSKPKSKRKRK"
     gene            complement(3844..4224)
                     /locus_tag="VGA92_09610"
     CDS             complement(3844..4224)
                     /locus_tag="VGA92_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9846697.1"
                     /translation="MIAEDNKFAARQYKLMLEQRGHTVITTENGEECVKKYKENLKKT
                     EFDSLDELPFDLVLLDHKMPKKTGARAAKEILAKNPKQKILFITGYQRWAIEDESDDL
                     IDKIILLEKPFTLSQLERKINSFK"
     gene            4530..5564
                     /locus_tag="VGA92_09615"
     CDS             4530..5564
                     /locus_tag="VGA92_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9846698.1"
                     /translation="MKILYIMIFLFGFATSPAFSQPTTDTIGTEPSPASHDGLLSVVF
                     VDLQTKGESILVIFPNGKTMLIDGGMPSSYPKLESVLKQHNISEIDVMVGTHADQDHI
                     AGLTKVLDDSDFTVKQVFISYVPSSTLTYQNFLNELSDNGLQSQAVFDGHVIDLDSAV
                     TAKIISPPIGGIQDGPSASLSNSNSLVTLLEYGSVSFLLTADATHTTESWIVQNHPDL
                     DIDIMNGPHHGSKYSSTDAFIDHLTPQLVIFSADQNNEYGHPHQEVIDEYQSRGINHY
                     QTGLEGSIVIKTDGVGCSLILEGEPEQPCYSGIQVVPEFPLVLAILFASVFVVVLFSK
                     FVVLRNPILK"
     gene            complement(5566..6720)
                     /locus_tag="VGA92_09620"
     CDS             complement(5566..6720)
                     /locus_tag="VGA92_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846699.1"
                     /translation="MYAIVVVILFSIFQTAFVPSEITIKEWDIPTPDSAPHDIVVGKN
                     GLVWFTEIATNKIGQFDPKTEQFKEFQISTPSSRPHGLVVDDGGNVWFTEVGAGKIGM
                     LDPQSGEILEYATPTPNSGPHTPIFAKDGILWFTEQRASKIGKLDTKNGEMAEYPTLT
                     PSANPYGIIVDAEGNPWFAELQGHNIGKVDAKTGQVTEYAPPTEKSGPRRIAIDSNDV
                     LWFTEYNSGKIGSLDPKTQEMIEYDTSSGSSGPYAIWVDKFDNVWFSMTGIYKVGKFD
                     QATQTLHEYDLPSPQTHIKFIHIDDSGNVWFPNYNNNKIGVILVSKEITKSEFGHLAP
                     HHQVKNGIAPQEVQCISSLELIFKSTDGSPACVTPQTKVMLIDRGWAKPI"
     gene            7188..7616
                     /locus_tag="VGA92_09625"
     CDS             7188..7616
                     /locus_tag="VGA92_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9846700.1"
                     /translation="MQQKLTAIVVDDDIDLVSVFCEYLKIMDVDVVGRGYDGKEAVDL
                     YEQKRPDIVFLDLSMPQYDGIFAVQRIKEANPDANLVVLTAYSSEDVRKKVERLGSEV
                     IIKPFEMDKVMDVIENVKNQVMQSPSIHRAEKHILKEVPG"
     gene            7613..7975
                     /locus_tag="VGA92_09630"
     CDS             7613..7975
                     /locus_tag="VGA92_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846701.1"
                     /translation="MTRRYLDRKTKEHFKKLVRYDDEPKHITEIGPKIPEFDEAIKAP
                     RALHLADVEDVQFDPDDILTIKIMELVNAAKETKEYIEQTNEHLDEQTKHLDELKNAH
                     RAFEKEIKSLKTKTEPEG"
     gene            8348..8611
                     /locus_tag="VGA92_09635"
     CDS             8348..8611
                     /locus_tag="VGA92_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846702.1"
                     /translation="MPNTVLFSRQQIKKHLIVIIHVTNPLDDTLEALQNIQDAINTVK
                     TRKTVISEKQILQDYDKIISHLEDGAASLLNAVVIFQKHERPL"
     gene            complement(9093..10922)
                     /locus_tag="VGA92_09640"
     CDS             complement(9093..10922)
                     /locus_tag="VGA92_09640"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX9846703.1"
                     /translation="MLTKTAFYGILFALITLGMTLFGTMPEASAVGGTISDQTSCEGM
                     GGSWTAPDTCTVTSLTINSGQTLTIPNGIMLVISNTGGAGIVNFGGTIDNSGTITVSN
                     SGGTGIVNSGTIGSSTINNSGTITVSNSGTSTGISNSRVISNSGTITVSNNGTIINTG
                     TINNSGTIDNNGTINNFSTIDGSTINNSGTITNSGTIINTSASTIDNSGTITNTDLST
                     IVNKGRITNSDTIDNSGTIDTSDTISNSGTITNSGTINNGFDNAFGVISNSGTITNLG
                     TINNIVGIIDNTSGTIIHQCGAVYSGTAPLGDPSNFISVCSNILINDVTLAEGNSGTT
                     SFDFIVIRSGDTTGTSTVNFATADGTATLADSDYDINSGLLTFTDGQTTKTVSVLVNG
                     DVVVEPNEQFLVDLSNCVGCIITDPQGIGTITNDDSPPSLSCGAGTVEVNSQCVAEPL
                     SDSDGDGFPDIFDQCDSDPLKIIPGINGCGTPEPDFDQIISDLQNQVNDLTQQVDDLT
                     SQINDLISQLDTIPSGIISSLIADVQSLVSDDTLDEKDSKKIIKELENSLKDLEKGNI
                     KACKDVSKFVKDIDQLIKKGKLSQADAQPLLDEANALLGSCPA"
     gene            complement(11195..11287)
                     /locus_tag="VGA92_09645"
     tRNA            complement(join(11195..11223,11244..11287))
                     /locus_tag="VGA92_09645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11252..11254),aa:Val,seq:cac)
     gene            11358..13124
                     /locus_tag="VGA92_09650"
     CDS             11358..13124
                     /locus_tag="VGA92_09650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX9846704.1"
                     /translation="MYKQGRWNLSELAISHKNPQFAKQITDLEFRVRKFEKLKTRLTP
                     SISSKDFFKIIRNIEEISERASKIAGYSSLLYAADTQSDEATSLVTKMAKLGSEIDNK
                     TLFFDLWWKQKIDEKNAKRLMKDAESLKEYLRHKRLLAKYTLTEPEEKIINTLDVTGS
                     TALVKLYDKITNAFEYVITINGKKKKLTREELTVLVRSTNAKTRQLAYKTLLTKYHHG
                     KGVLGEIYQNLVLNWKDEGIEIRGYSSPISIRNIANDVDDKTVQSLLEVCKDNAGIFY
                     EFFSYKAKTLGLKRLRRYDLYAPNVKKIKEKNYPYQKAASLVLESLAKFSPNLSDFEK
                     SVFEENHIDSEVRPGKRDGAFCSTVTPKITPYVLVNYTGKSRDVFTLAHELGHAVHSK
                     AASDQSILVSEASLPLAETASTFSELLLYDNISGQMSADEKRITLSEKIDDLYATIMR
                     QAFFTIFEVDAHRQIADGTTVDQISKTYLANLKSQFANSVDLSDDFAIEWSCIPHFFH
                     TPFYCYAYSFGNLLSLSLFQRYKKEGNEFAKTYTEILASGGSKKPEDLLSEHGIDISS
                     KKFWQDGFDYVRSQVKELVLLN"
     gene            13295..14428
                     /locus_tag="VGA92_09655"
     CDS             13295..14428
                     /locus_tag="VGA92_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846705.1"
                     /translation="MYWVFVVFLLGFAFIGYAFAEEPRLATYHEIATVIVDQRFSNNV
                     TASISLQTTSIQEFLVPPDLDLKIRNNTDITAVIITNEDQCILGVDDQICIMVNTQRI
                     QGEGIRAIQEKARHAGDSLIDDINETFDLNAKFHSIFVHYEDSANEALNTTGEISGAG
                     TVSAVYTAQMQSTDFMFNKISGVLIPRQIRGMGGFYDVAQELSKDDSSRMTFTIFPKG
                     EVSIMQLKVSEKYPNIAKDVSIVDPLMFLKVDQIKKSSYFSVGFFPLNSLIHVIVLPE
                     KEINEVHVTNVIEPTIKDGQKIPSDLSKNGWFFDSESGEKIDAMYLFGEGLSANRQDL
                     MLVIGDEQEELQTGFNETYILIGIGAAAAGAAAFYLKGIKKKN"
     gene            complement(14425..14976)
                     /locus_tag="VGA92_09660"
     CDS             complement(14425..14976)
                     /locus_tag="VGA92_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HEX9846706.1"
                     /translation="MLDLVAKKLFLTKGKGVHEDRLTSFEYALRDAGISGTNIVLISS
                     IFPPGAKLVPRAEGLKLIRAGQVLFTIYSRNQTDEPHRLISASVGIAQPSDTKRYGYL
                     SEYEAFGQNEKKAGDYAEDIAAQMLASSLGIKFDIDKSWDEKRQQWKISGQIYKSMNV
                     TQTAVGDKDGRWTTVFAAAVLII"
     gene            complement(15014..15472)
                     /locus_tag="VGA92_09665"
     CDS             complement(15014..15472)
                     /locus_tag="VGA92_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9846707.1"
                     /translation="MREGDAAPDFELPASDGTTVKLSSFSGSKNVVLCFYPKNHLFMC
                     PSKKVFKMAQSVISAYSDILKTGSVLFAISVDTADDQKKFVSEYQIPYLHLSDTAKNA
                     CKTYAGLNVAGLSKRSTFVIDKNGIIRKIFRDVDVEKHGEDIARTLAELK"
     gene            15520..16092
                     /locus_tag="VGA92_09670"
     CDS             15520..16092
                     /locus_tag="VGA92_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846708.1"
                     /translation="MLDFSFLFTLDSIFSSEGFIIGYYVFTVATSLILIKETKKRVFD
                     LAKGIKSMVYAPISFGILILYVVFAYPLVEEIPILNWSWLGYNIAFGPFAEEGFWGIV
                     PFIPILIYMFIHINYVEEFYFRKSKKMVLVWAAAHIAMGIKVHMAILLIPIGFLFKYL
                     YDKKGVEHSYAMHFATNMLVVTALFFTLVR"
     gene            complement(16054..16278)
                     /locus_tag="VGA92_09675"
     CDS             complement(16054..16278)
                     /locus_tag="VGA92_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846709.1"
                     /translation="MKYIPPKKLKIIMIMFFAGGIWGIVSGLFIIKPFVFFIVVFGVI
                     NLCLGGLFGYRLLTQVKPSPDERKKKRGNN"
     gene            16365..16438
                     /locus_tag="VGA92_09680"
     tRNA            16365..16438
                     /locus_tag="VGA92_09680"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16399..16401,aa:Thr,seq:tgt)
     gene            complement(16481..16876)
                     /locus_tag="VGA92_09685"
     CDS             complement(16481..16876)
                     /locus_tag="VGA92_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX9846710.1"
                     /translation="MKRYGKLIVKGVKESLENIPFHGKAPIKRLSMLSKKLVQKSDTH
                     VAVHFIDSLPKKIPAYSKLHKHNVDEINLILSENDKLTYEIQLEDEIYKVTSPSTIFI
                     PKGVRHRAQVISGKGLFVCIILSSNYNSS"
     gene            16969..17658
                     /locus_tag="VGA92_09690"
     CDS             16969..17658
                     /locus_tag="VGA92_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846711.1"
                     /translation="MQQDRTSEWFVPKFGPRNFRVSIGILFLPYTSIVTCFAAWGALS
                     TSFELERLVAICAIYFLAVGVSAHCLDAVGGKTKPWGELPKRKLWAIATTSLGVAFVI
                     GLYYAFLDSPLLFPIGIAEGFFLFAYNLELFRGKFHNNASTVLSWGVLPVFAGSAIQT
                     NSITLEAILLSAVSGIITYLLIITSRRYKELKRDSQDHIQIKKKELVLKLIFIGVVCS
                     TAAFVIVRTYL"
     gene            complement(17669..18352)
                     /locus_tag="VGA92_09695"
     CDS             complement(17669..18352)
                     /locus_tag="VGA92_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9846712.1"
                     /translation="MAAESPKNLVPKFFSNTATTYDKIATWATFGKDRYWKKEILRQI
                     PKADSYLDLACGTGILTRKIAEQFPFAKIVGVDISENYLDVAKKNSKSYQNISFVLQD
                     AEDLSLSAKFDCVVSSYIPKYCDAGILVTACLAHLNDGGKIILHDFIYPKNKLVQKLW
                     KMHFVMLRLAGLFLPSWSDAFSELPNLIKTSRWLDDCESEMKKHGLAVTKQYLTCSTS
                     AILTGNLKT"
     gene            18503..20071
                     /locus_tag="VGA92_09700"
     CDS             18503..20071
                     /locus_tag="VGA92_09700"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9846713.1"
                     /translation="MTTFEQLGIKKSIIDGLKEIGFEAPFPIQEATIPVLLSGRDVIG
                     QAHTGTGKTAAYAISMLQNIEPTKRIQGLILAPTRELAVQITTEFKKFAKYTGVKIVS
                     IYGGQGIGIQHQALKHGVEIIVATPGRLIDHLRQESIELNDVTYVVLDEADTMLDMGF
                     IDDIKFILELTPVNKVMSLFSATMPPEILRLAEEYLNNPKQFLLDADDLSGEGIDQSF
                     LVIKDREKMSYLTQFIKENKKGQTVVFCSTKNRTRDVARELKHNGFNAVAIEGDMSQH
                     RREISMSQFRRGGADILVATDVAARGIDVPQVALVVNYDVPNQELIYFHRIGRTARAG
                     AEGRAITLVSYSSIGDFNIIKQQTKVNMTDLNEKLGIVVSIPDPLKRQVGQRRTFGRG
                     GGDRRQSYGRPDSRRPSYGRSDNRRPSYGRSDDRRPQYGRSDDRKPSYGRSDDKRPQY
                     GRSDDRKPSYGRSDDRKPSYGRSDDRRPSYGKSENRGSYGRSDDRRTEYGKSDNRRGP
                     MRNAVRRPNHRRRW"
     gene            20104..20349
                     /locus_tag="VGA92_09705"
     CDS             20104..20349
                     /locus_tag="VGA92_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9846714.1"
                     /translation="MEIGFVLLNCDLGAEEYLLEEIKQVPEVKQAYLTFGAYDIIIEI
                     HAKSADEFDKTVSSKIRRLSRVMSTMTLKVLSTAEKH"
     gene            20375..20662
                     /locus_tag="VGA92_09710"
     CDS             20375..20662
                     /locus_tag="VGA92_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846715.1"
                     /translation="MEKIEYTGTVWAINHNNPEPLVEHCLKKLQQYGVKKEDIRLTDA
                     PENVKIGEIVVEIWPYHLDVARVRTIRNESFISGLVMQIELKADEQGNYID"
     gene            complement(20717..21559)
                     /locus_tag="VGA92_09715"
     CDS             complement(20717..21559)
                     /locus_tag="VGA92_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846716.1"
                     /translation="MKSLIAIPIFALAILFTQSYAQETDYFLSASGYKISNDAANKMT
                     LDFQLEMTSPANPVLRQGTLVLDDKERAIENFNLIFYRDGRLFRLNAESDDLTIRANG
                     RMLVSNQEGSIYQLSGATTRDGVSERFLLVAILKPIEEQKTVDFAAILPEKETVAEKE
                     DVLVLVRHVDRVEWMGLYKFTVRTFDPLLNSFSDFYKTSGYLEGVPISAKITDPLGNV
                     IETSSGETQKFGYYEASLLIPDNSRTGAYVLNVTVSGEKFKASSKEMTFFVTPPRSDG
                     SSTP"
     gene            21673..22158
                     /locus_tag="VGA92_09720"
     CDS             21673..22158
                     /locus_tag="VGA92_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846717.1"
                     /translation="MKSTYLVVGFGVIIVAIAVFYFYSADQAKIRGFNFGNSLDAVQG
                     ELKQVQTDFYSKKRLLDENTITKEEFLEFSEEHVEKMEEMVSKYESLVSPNSFVKAVE
                     LFKTSTQKQLESDRFLIEWIRTNDTSDRVRSDLLLQESFENEMAALSSFEKAKNSSGQ
                     D"
     gene            complement(22155..22607)
                     /locus_tag="VGA92_09725"
     CDS             complement(22155..22607)
                     /locus_tag="VGA92_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846718.1"
                     /translation="MWKIEREADFFRIFDKDQKIAGYFVPEYGDIQPEEKIEEIIEQM
                     HKNGDKVPGGYMTVPMVKFGIFDSNDDLDIVYLQGQLADASARLDAWDEFLSQNQMRH
                     AIRMSHTDSDMLSLTFPIKFSDQIPLDKKIILETIAPTLDLLHQKGLL"
     gene            22664..22939
                     /locus_tag="VGA92_09730"
     CDS             22664..22939
                     /locus_tag="VGA92_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846719.1"
                     /translation="MAITKYTKDFLDGLIDYYISEAPSYKQIAESYFPEITSVEDAAF
                     GIIAGCIYSAFLRAYESENKPVELDDMQEFNKIIKERAALIKKAILE"
     gene            22971..23291
                     /locus_tag="VGA92_09735"
     CDS             22971..23291
                     /locus_tag="VGA92_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846720.1"
                     /translation="MGDLTRYRIHQIVDNGQIVQLALAEDVQTEPMSQKQLIMDSVAK
                     KLDPEVKEQVQPLLEAILQAQPTIKIKSYEQTTISITMPRARYERMGRPQVGEVLEIN
                     ISRK"
     gene            complement(23280..25313)
                     /locus_tag="VGA92_09740"
     CDS             complement(23280..25313)
                     /locus_tag="VGA92_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9846721.1"
                     /translation="MAQNHLIHETSPYLLQHAHNPVGWYPWCEEARQKAKTENKPIFL
                     SIGYSSCHWCHVMEHESFENEEIAKIMNENFISIKVDREERPDLDDIYQKVCQMSTGQ
                     GGWPLSVFLTPDQKPFYVGTYFPILDSYGRPGFGSILRQLAQAWKEKPSDVKKAAESF
                     MTTLQQAEHVTTPAKLEKTILDEAAVNLIQIADNTYGGFGSAPKFPNAANLSFLLRYS
                     KISKISKFSEFVLKTLTKMARGGIFDQVGGGFHRYSTDARWLVPHFEKMLYDNALLPI
                     VYAEAYQITKDPFYLDVIKKTLGFVLREMASKEGGFYSALDADSEGEEGKYYVWKKKE
                     IRDILGADAEVFFLYYDVTDGGNFEGNTILNNNISTSSVAFHFGITEDKVRQIVSEGE
                     KKLLDARAMRIRPGLDDKVLTSWNALMISAFAKAYRVTDDKSYLAAAESCISFIEKNL
                     ITEGQLLRTYKDGKAKIHGYLEDYAYFVNALIDVFEANPQAKYLELATNLANYLVDHF
                     WDAEHNSFYFTADNSEQLIIRPKNNYDLSMPSGNSVAALALLILYHLTQQKSFLEIST
                     KIMESFAIMAAENPFGFGFLLNTIYLYLQTPTEITLINTKNKELYDFVTKKFLPETIL
                     VTIQNADQLKQLSKHAFFAGKEFDDSKTAVFVCKNFSCSLPLATIPEIENLLS"
BASE COUNT         7572 a         5265 c         5645 g         7092 t
ORIGIN      
        1 aaaacatcgc ctcccaagta gaacggatgc tagagactac aaccgacatt gtttattttg
       61 ttaccaccct tgaggacata tcgaggctat atcattctga gataccagaa aagatcaagc
      121 ttgccgaaaa ggcaggcgga accgtgcggc tcatcctaga tgaggtatcc cccaagatgc
      181 taccatttgt caaaaggttt ggggcaagcg aggtcagatt gggcaagctc ccatccaagg
      241 gaagaatagt agtttccaag aactgcccga ttactggcaa acttttgatg tcggattccg
      301 cagtgagggg aacactgcac gagaacattg acgcagagtg cgcgctgttt actaattctg
      361 aggagatgac aaacaacatc tttactttat gcaccttcat ttggaagggc gcagacgtca
      421 tcaagctgta agctcaaaaa aataaataat gcaaaaatca cctcacagag gtcttgttta
      481 taattaatta caaaaattac gaggagatct ctggcgacaa ggccgcaaag cttgccgccg
      541 ttgcctcatc aatatcaaga aaatacaagg ttaaaatcgc cattgccccg ccacaccacc
      601 tcttatctgc tgtgaaaaat tattcagggc caatcctggc gcagcatgtg gataatgcca
      661 gagttggcag cacgacaggt tttgtcatac cggaattgtt aaaaaaatca aaaatcagcg
      721 gctcactcat caaccacagc gagcacagga ttccaagcaa ggacatagtc gaattggtga
      781 atcgattacg aaatctaaaa atgatatctg ttgtttgcgt caaggacgta ccagaggcag
      841 ctaaatacgc aaagctaaat cccacattca ttgcaataga gccccctgag ctgattggga
      901 ccgggagagc catatcaaca gaaaggcccg agctgataac aaaggcagta caagcagtaa
      961 aggcggcaaa aaacaaaaca aagctacttt gtggtgccgg aattgttact ggcaaggaca
     1021 tcagaaaggc aatcgagctg ggctcagagg gcatacttgt ggcaagcggc atagtcaagg
     1081 caaaaaactg gaataagatt gtcgaagagt ttgctaaagg aatgcttaaa atcagttcaa
     1141 atcgatagga aaatcctgat gaaaaaaatg aagatggtgt atttctttga tgagggagac
     1201 ggcaagaaca aaaagctcct aggcggcaag ggcgcaggcc tctgcgagat gaccaggcta
     1261 aggctaccag ttccgcccgg atttacaatt actaccgaag tctgcaagac gtattatcaa
     1321 aataacaaaa cgctgccaaa gtcgctttgg tcagaagtca aaaaaaatat tgcaaaactt
     1381 gaaagaagga ccgggaaaaa atggaactct aatcaaaacc cgttgcttgt atcggtgcgt
     1441 tctggcgcgg cactctcaat gcctggcatg atggatacga tactaaactt gggcctaaat
     1501 gatagcgctg tcgagtcatt gacaaaaaag acaaacaacc cgaggtttgc ctgggactcg
     1561 tatcgaaggt ttgttcagtt atttggcaaa gttgtatttg gtgtaaatga caaaaagttt
     1621 gacgaagtcc tagatggggc aaagaaaaag caggggattc atgaagacag ccatctgagc
     1681 gagcaatccc taagggaggt agttgcacaa tacaagcaga tctgccaaga ccacacagga
     1741 aagccgtttc catcacatgc agacgagcag ctacagcttg caataagggc agtctttaac
     1801 agctggatgg gcgagcgggc cattgtatat cgagagaaaa accacataac aaaagacatt
     1861 gcagacggga ctgccgtaaa tgtggtcgcc atggcctttg gcaatatggg agatgatagc
     1921 gcaactggcg tggtatttac gcgaagccca gctgatggca caaacaagat ttttggcgac
     1981 tatctggtaa atgcgcaggg cgaagacgta gtagctgggg tccgaacgcc aaagcctgtg
     2041 gacgagatga aaaaagagtt gccaaaaggc tacaagcttt tgctagatac gtgcaaaaaa
     2101 ttagaaagac actacaagga accgcaagac atcgagttta caatagagca aggcaagttc
     2161 tacctgcttc agactaggtc tgcaaagatg aacgcagtcg gaatgataaa gacctcagtt
     2221 gacatggtaa aagaaaaact gatcacaaaa gaccgtgcgt tattacgact gcagccggat
     2281 cagctcgagc agatgttgca caaaacaatt gactatgaaa aggcaaggca gcacaaaaag
     2341 gtaacaaagg gaattgccgc gtcccctggt gctgccagcg gcgttgcagt ctttgacgta
     2401 aagcgcgcag tagcaatggg agaaaacggt gccaaagtaa tcttggtaag agaagaaaca
     2461 aaacccgagg atgttccggc gtttttcaca tcagttgcaa ttctgactag tcggggcggc
     2521 aagacatcgc atgcggcagt agtcgcaagg ggaatgggca agccctgtat agtcggctgc
     2581 tcggatctca aaataaacgc cgacgcaaag caggcaacca ccggagatgt cgtaattcat
     2641 gaaggcgacg caatcacaat cgatggaagt ataggcgaag tgtacctagg tgaggtacct
     2701 acaatagagc cgcaaataac tgatgatttt aggacaattc tgagctgggc gcaaaaggca
     2761 aaaaagattg gaattagagc aaacgctgac accccagatg gtgcgcagct ggcaagaaaa
     2821 tacggcgccc agggaatagg actgtgcaga accgaaagaa tgttcaacgc aaaagaaagg
     2881 cttggacttt ttgttaatct aattatggca aagacattag aggagagaaa actccacttg
     2941 caaaagctgc aggagctgca aaaaagcgac tttgtacaaa tcctgaaggc aatggagggc
     3001 tacaaggtca caatacgact cttggatcct ccgctccacg agtttttgcc aaatcctgaa
     3061 gagctaatta acaaaatcca caggctcgaa aaacaaggaa gctcgcagga attagaggaa
     3121 gtcaagattc tgcttgccag agcaaaggag cttgcagaag taaacccgat gatggggcac
     3181 aggggcgtcc gagtcggagt cacgtatcca gaaatctatg agatgcaaat tagagcagtc
     3241 tttgaggcag cagctgagct tgcaaaacaa aaagtgcacg cacatcccca gattatgatc
     3301 ccacaggttg gaagcgtagc tgagctaaac cacatcaagc aaatttacaa caaagtcaag
     3361 tcagagatag aatcaaaaca ccgcatgcga cttccgataa actttgggac aatgctcgag
     3421 gtggtccgcg gctgccttac atcaggcgag cttgcacaga ctgccgagtt tttcagcttt
     3481 ggcacaaacg atctcaccca agcagtgttc agctttagca gggaagatgc ggaaggaaaa
     3541 ttcctgccag aatatttaga aaaagagatt cttgagcgaa acccgtttca gacagtcgat
     3601 gtaaacggcg taggcagcct agtcaaaatc gcaattgcaa acggcaaggc agtaaaaccc
     3661 agcttggaga tcggagtctg tggtgagcat gggggagacc caaactctat caagttcttc
     3721 cacagtgtcg gagtatcata tgttagcgcc tcaccgcaca gaattccaat tgccatagtt
     3781 gcagcagctc aggcaaacct gattgccaaa tcaaagccca agtcaaaaag aaaaagaaag
     3841 taattactta aacgagttta tctttctttc cagttgtgag agtgtaaacg gtttttccaa
     3901 aagaattatc ttgtctatga ggtcgtctga ctcgtcttct atcgcccacc tctgataccc
     3961 ggtgataaag aggatttttt gttttgggtt ttttgcaagt atctcttttg cagcccttgc
     4021 accagtcttc tttggcattt tgtggtcaag cagcactagg tcaaagggca attcatcaag
     4081 tgagtcaaac tcggtttttt tgagattttc tttgtatttt tttacacatt cttcgccgtt
     4141 ttccgtagtg atgactgtgt ggcccctttg ctcaagcata agcttgtact gccttgcggc
     4201 aaacttgttg tcctctgcga tcaagatgtt caatttgact agaatgtctg gtaatgacgt
     4261 ttgaaagcta gtgtgttctt tttgaacaac atttcaaaaa tgttcacgaa taattcctac
     4321 gcaagccttt ttttattaga aatcacatct tgagcaggtt cattcgaaga caaacgtgcc
     4381 gagattagaa ccagccaaga ccaaccaaat cagagtgagg atatccacaa gtcgaccatg
     4441 ttctacctga tatcctcact tttttcattt aactgtagat tttgatacct gtcagcaaac
     4501 ctaaattctt gctttcctaa tttagtacta tgaaaattct ctacatcatg attttcttgt
     4561 ttggttttgc caccagtcct gcattttctc agccaaccac agacacaatt ggaacagagc
     4621 caagtccagc ttcgcatgat ggcttgctaa gtgttgtctt cgttgatctt caaacaaaag
     4681 gcgaatctat tcttgtgata tttccaaatg gaaaaacaat gctaatcgat ggcggaatgc
     4741 ctagctcata cccaaagctt gaatcagtcc taaaacaaca caacatatcc gaaattgacg
     4801 tcatggtcgg aacacatgct gaccaggatc acattgcggg cctaacaaaa gtcttggatg
     4861 attcagactt tactgtcaaa caagttttca tatcatacgt accaagcagc actttgacat
     4921 accaaaattt tcttaatgag ctttcagata atggtttaca gtcgcaggcc gtctttgatg
     4981 gtcatgtaat agatcttgac agcgccgtca cagccaaaat aatcagccca cccattggag
     5041 gaatccaaga tggtccaagt gcaagtctat caaactcaaa ctctcttgtc actttgctag
     5101 aatatggaag tgtttcgttt ttacttacag cagatgcgac acatactaca gagtcttgga
     5161 ttgtgcagaa ccatccagat ttggacatag atatcatgaa tggtccccac cacggctcaa
     5221 aatactccag caccgatgca ttcattgacc atctcacacc acaactagtg atattttctg
     5281 cggatcagaa caacgagtac ggccacccgc accaggaagt cattgatgaa tatcagtcga
     5341 ggggaattaa tcattatcag acaggattag aaggaagcat cgtaatcaag acagatggag
     5401 tcggatgttc gctcatacta gaaggcgaac cagagcagcc atgttattca ggcattcaag
     5461 ttgtgccgga atttccgctt gtcttggcaa ttttgtttgc ttctgttttt gttgttgtgc
     5521 tattttctaa atttgtggtg ctgcgaaatc caattttgaa ataactcaga ttggttttgc
     5581 ccagcctcgg tcaattaaca tcaccttggt ttgcggtgtt acgcatgcag gagagccatc
     5641 tgtcgatttg aaaatcaatt ctaaagagga tatacattga acctcttgag gcgcaatgcc
     5701 gtttttgact tggtgatgtg gtgctaggtg accaaattcc gactttgtta tttctttgct
     5761 gactagaatc accccaatct tgttgttatt gtaattagga aaccagacgt ttccactatc
     5821 atctatgtga atgaatttga tgtgtgtttg aggcgacgga aggtcatact cgtgcaatgt
     5881 ttgtgttgcc tgatcaaatt ttccgacctt gtatatgcca gtcatgctaa accacacatt
     5941 atcaaatttg tcaacccata tcgcgtatgg tccggaggat cctgagctgg tatcgtactc
     6001 gatcatttct tgtgttttag gatcaaggct tcctatcttt ccagaattat attcggtaaa
     6061 ccataacaca tcattactat caattgcgat tctccttggc ccactctttt ctgttggcgg
     6121 cgcatattct gttacttggc cagtctttgc gtcgactttg ccaatgttat gtccctgcag
     6181 ctctgcaaac cacgggttgc cctcagcgtc gacaattatc ccatagggat tcgctgatgg
     6241 cgtaagggtt ggatattcag ccatctcacc attttttgta tcgagctttc caatcttgga
     6301 ggctctttgt tctgtgaacc ataaaatccc gtctttagca aatatcggag tgtgaggccc
     6361 cgaatttgga gtcggtgtcg catactccag tatctcaccg ctttgagggt caagcattcc
     6421 tattttccca gctcctactt ctgtaaacca cacatttccg ccatcatcga ctactaagcc
     6481 atgcggtctt gatgatggtg tagatatttg aaattctttg aactgttctg ttttaggatc
     6541 aaattgtcca atcttgtttg tggcaatctc tgtaaaccac actagcccat tttttccaac
     6601 aactatgtcg tgtggtgcag agtctggtgt cgggatgtcc cattctttta tggttatttc
     6661 agaaggcaca aaagcagttt ggaagataga aaataggatc acaacgacaa ttgcatacat
     6721 cattaaatta ttcgcgacat gacatatatg atttagtcat gattggtgtg atgcgttgca
     6781 ctcgtgtgat atatttaata atgatttttg ccataaaata atgtggattg gttttttctt
     6841 cttctagcac taattcatga atgctcggaa attcgcccgt aatagataat aaaagagaat
     6901 ttttttaatt atgtgaattt atgtccccag tacgacggct tctacgcact ggaaaacatc
     6961 aggaagatca accctgaagt ccgggtgata atcatcacgg cggacctgcg aaaggacacg
     7021 taacataaaa cttccaagct caggcatgag tgtacgcgcg gatatcacat ttcctgactt
     7081 tgtataatac cggctagtca ttacatcaag gtaggcattt catactatgt ggtttgtttg
     7141 aaaaattcag gcagaaccaa ttatgcagaa aaggagtaaa tccgctgatg cagcaaaaac
     7201 tgactgcaat tgtagttgat gacgatattg acttggtaag cgtattttgc gaatacctca
     7261 agataatgga tgtggatgtc gtgggccggg gctacgacgg caaagaagca gtagatctgt
     7321 acgagcaaaa aaggcccgac attgtatttt tggatctgtc aatgccgcaa tatgacggca
     7381 tttttgccgt acagagaatc aaggaggcaa accccgatgc caatcttgtc gtcctgactg
     7441 cctattcatc tgaggatgta aggaagaagg tggaaaggct tggctcagag gtgatcatca
     7501 agccgtttga gatggataag gtcatggacg taatcgaaaa tgtgaaaaac caagtgatgc
     7561 agtcgccttc aattcacagg gcggaaaaac acatcctcaa ggaagtgccg ggttgacaag
     7621 aaggtatctt gaccgcaaga caaaggagca ctttaaaaaa ctggtgcgct acgacgacga
     7681 gccaaaacat ataactgaaa tcggtcccaa aattccagaa tttgatgaag cgataaaggc
     7741 gccaagggca ctccatcttg cagatgtaga agacgtgcag tttgacccag acgacatact
     7801 gactataaaa ataatggagc tggtaaacgc cgcaaaggaa acaaaggaat acatcgagca
     7861 gacaaacgag cacctcgacg aacaaaccaa acatctagat gaattaaaaa atgcccacag
     7921 agccttcgaa aaggagatca aatctctcaa aaccaaaacc gagccggaag gctaggctac
     7981 aaggcaaagc cctgtcagat ggcttttttg tggacagaat ttttactgcg tttgactcaa
     8041 gccctttttt gaccagagag ctgtcatgac agctaacgcc aagaacatca ctttcacatc
     8101 agttcaacgc ctaaagttac gtaattatgc gataaaaagt gtgtatttcg tggaaaaatg
     8161 taaatgaacc gaatgtgttt tacaccgatt ttggtggacc cggagcaatt agcgcctcta
     8221 gcggtacacg ctttttgggt tccgatgttt aaacagcaaa gccagcttta aagttttcta
     8281 acagaaagta atcaaggaat aattcagtta tgtcgaactt atttcaataa gcaagtgcct
     8341 ctgattattg ccaaatacag tcttattttc tcgtcagcaa attaaaaagc atctaatagt
     8401 tatcattcat gtgacaaatc ctcttgatga tacactagaa gcattacaga acatacaaga
     8461 cgccatcaat accgtcaaaa ctagaaaaac tgtcatatca gaaaaacaaa tactacaaga
     8521 ctatgacaaa atcatatctc atcttgaaga tggtgcggcg agtttgctca atgctgttgt
     8581 aatatttcag aagcatgaaa ggcctttgta aaagactcta tggttccaaa ataattgcaa
     8641 ttcttgcacc tgtaatagac aattccgttt gagtgggtgg caagcatatc aggttcgttt
     8701 tttggacagt ccggattgaa atagtcgtgc ttgcttttca tgtcatcctt atacaaaatt
     8761 tgtataaaaa tatgacaccc aacaggctct ggtgcattga cgtgggctgt tattcgtgtt
     8821 gcaagtcggg ggatgatcgg ttaaacaagg catttttgac actgttcaat ctcagggtga
     8881 tttcaccaat ctgtcgacac tgttaattct taggtagatt tcactgactt tctctgctgt
     8941 taatttaggg tgatggagtg ttaagttagg ttgattttgt ggtgtgaaaa atagtgggtt
     9001 aagattcgga tgattgaatt atagataaag atgatgtggt gttaggaaaa caaaaagagt
     9061 ttagaattta gaaaataatc gttgtcagaa ttttatgctg ggcaagaacc aagtaaagca
     9121 tttgcctcat caagtaatgg ttgagcatcg gcttgtgata attttccttt ctttatgagt
     9181 tgatcaatgt ctttgacaaa tttgctgaca tctttgcaag ctttgatgtt tcctttttca
     9241 aggtctttaa gactattttc caactcttta ataatttttt tagagtcttt ttcatctagt
     9301 gtgtcatcac ttacaagtga ttgtacgtca gctattagcg atgaaataat tccacttgga
     9361 atagtatcaa gctgcgatat tagatcatta atttgactag taagatcatc aacctgttgt
     9421 gtcaagtcat tgacttggtt ttgtagatca gaaattattt ggtcaaagtc tggttctgga
     9481 gtaccgcatc cgtttattcc cggaattatt ttgagaggat cagaatcaca ttggtcaaaa
     9541 atgtctggaa atccatctcc atcagaatca gagagaggct ctgccacaca ttgactattt
     9601 acttctactg tgccagcacc acagcttagg cttggaggtg aatcatcatt tgttattgtt
     9661 cctattcctt gagggtcagt aatgatgcat ccaacacagt ttgataaatc gacaaggaat
     9721 tgctcatttg gctccactac cacatcaccg tttacaagta cagacacagt ctttgttgtc
     9781 tgtccatctg taaatgtgag taatccagag tttatatcat aatcagaatc agctagagtt
     9841 gcagtaccgt ctgctgttgc aaagtttaca gttgatgtac ctgttgtatc gcctgatctt
     9901 ataacaataa agtcaaagct tgttgttcca gagtttcctt ctgctagtgt tacatcatta
     9961 attaagatgt tagaacatac cgaaataaag ttactcggat ctccaagggg tgcagttcca
    10021 gaatatacag cgccgcattg atggatgatg gtgccggagg tattgtcgat gatgccgacg
    10081 atgttgttga tggtgcctaa gttggtgatg gtgccggagt tgctgataac gccgaaggcg
    10141 ttgtcgaagc cgttgttgat ggtgccggag tttgtgatgg tgccggagtt ggagatggtg
    10201 tcgctggtat cgatggtgcc ggagttgtcg atggtgtcgg agttggtgat tctgcctttg
    10261 ttgacgatgg tgcttaagtc ggtgttggtg atggtgccgg agttatcgat ggtactggcg
    10321 gaggtgttga tgatggtgcc ggagtttgtg atggtgccgg agttgtttat ggtgctgccg
    10381 tcgatggtgc tgaagttgtt gatggtgccg ttgttgtcga tggtgccgga gttgtttatg
    10441 gtgccggtgt tgatgatggt gccgttgttt gatactgtga tggtaccgga gttggagatg
    10501 acgcgggagt tggagatgcc ggtgctggtg ccggagtttg atactgtgat ggtgccggag
    10561 ttgttgatgg tgctgctgcc gatggtgccg gagttgacga tgccggtgcc gccggagttt
    10621 gatactgtga tggtaccaga gttgtcgatg gtgccgccga agttgacgat gccggcgccg
    10681 ccggtgtttg atattacgag cattatgccg ttaggaattg tcagcgtttg tccactgtta
    10741 attgtcaaac tagtcacagt acaggtgtct ggtgcagtcc acgaacctcc cataccttca
    10801 catgaagtct gatcagaaat tgtcccgcct actgcagagg cttctggcat tgtgccaaat
    10861 aatgtcattc caagtgtaat taaagcaaat aaaattccat aaaatgctgt tttagttagc
    10921 aataaagccc ggatcgatta taagtattaa agtgttgcat ggaaaatggt aatgattgtg
    10981 cctttttttg aaaaattttt cataaaaaaa ttctaacgtc gactcgaagg gaatcgaccc
    11041 tctaggttca gacctcctag gatttgaacc cataacataa taatttttac aaaaattaat
    11101 tacacgtttc taaatcattt catagaattt gtaaattaag gttaaaaatt tctacaaaaa
    11161 tatggttcag ctaactagtt agttagaatg tgagtggacc gaatgggatt tgaacccatg
    11221 acccttgcgg gattatacat ccatacgcag tgtgagtgcg tcatcctaac cactagacaa
    11281 tcggtccaac ttttctgaaa aaatgcagta tttaggcgtt agaaatatca tatacgggca
    11341 attcagaaca agatcaaatg tacaaacagg gacgttggaa tctctcagag cttgccatca
    11401 gccacaaaaa cccacaattt gcaaaacaaa tcactgatct ggaattcaga gtaagaaaat
    11461 ttgaaaaact aaaaaccaga ctcaccccaa gcatatcttc caaagacttt ttcaaaataa
    11521 taagaaatat tgaagaaata tcagagcgtg caagcaagat agccggatat tcgtcgcttt
    11581 tgtatgcggc agacacgcag tcagatgagg caacatcgct tgtaacaaaa atggcaaagc
    11641 tcggctcaga aattgacaac aagactttgt tttttgattt gtggtggaaa caaaaaatcg
    11701 acgaaaaaaa cgcaaaacgt ctaatgaaag atgcggaaag tctaaaggaa tacctaaggc
    11761 acaaacggct tttggcaaaa tacacgctaa cagaaccaga ggaaaaaata atcaacacac
    11821 ttgatgtcac cggctcgact gcgctagtca agctatacga caagataaca aacgcatttg
    11881 aatacgtcat aaccattaac ggaaaaaaga aaaagctcac ccgcgaggag ctaacagtac
    11941 tagtcagaag cacaaacgca aagactcgtc agcttgcata caagacgctt ctcacaaaat
    12001 atcaccacgg taagggcgtg ctaggtgaga tttatcaaaa ccttgtatta aactggaagg
    12061 acgagggaat cgagattaga ggctactcat ccccaatatc aattagaaac attgcaaacg
    12121 atgtggatga taaaacagtg caaagccttc ttgaggtttg taaagataac gcgggtatct
    12181 tttacgagtt tttctcctac aaggcaaaaa cgcttggact caaaagactg cgtcgatacg
    12241 acctatatgc cccaaacgtc aagaagataa aagaaaaaaa ctatccatac caaaaagcag
    12301 caagcctcgt tttggaatcg cttgccaagt tcagccccaa tctctcggat tttgaaaaat
    12361 ctgtctttga ggaaaaccac atcgactctg aagtcaggcc gggaaaaaga gatggcgcgt
    12421 tttgcagcac agtcacacca aaaataacac cttatgtcct agtcaactat actggaaaat
    12481 cgcgcgacgt atttacgctt gcgcatgagc tcggccatgc agtacacagc aaggccgcat
    12541 cagaccagtc cattctagta tccgaagcgt cacttcctct agccgagacc gcatcgacat
    12601 tttctgagct gctactttat gataacatat caggccagat gagtgcggac gaaaaacgaa
    12661 taacactctc agaaaaaatt gacgatttgt atgccacaat aatgcgccag gcattcttta
    12721 ccatttttga agtcgatgca cacagacaga ttgccgatgg caccacagtc gaccagatat
    12781 caaagacata ccttgcaaac ctcaagtcgc agtttgcaaa ttctgttgat ctctcagatg
    12841 actttgccat agagtggagc tgcattccgc actttttcca cacgccgttt tactgctatg
    12901 catattcgtt tgggaacttg ttgtctttgt ccttgtttca aagatacaaa aaagaaggca
    12961 acgagtttgc aaagacatac actgagattt tggcatctgg tggatcaaaa aagccagaag
    13021 acctgctaag cgagcatggc atcgacataa gctccaaaaa gttctggcag gacggatttg
    13081 actatgtaag atctcaagta aaggaattag ttttgttaaa ttaaatgggg ctggcagcct
    13141 ttacgcacgg gctgccagca tttgttcccc ttcggtttga attcgatgtc agagtgtgaa
    13201 attaaattaa agaatttaaa cgtttgactt tggggcaatc tgaaatgaca aattttgagt
    13261 aaagttaata ctgaaaaaca aggcttggca agatgtgtac tgggtttttg tcgtattttt
    13321 gctcgggttt gcatttatcg gatatgcttt tgccgaagag ccacgccttg ccacatatca
    13381 cgagatagct actgtaatag ttgaccagag attctcaaac aacgtcacag cgtccatatc
    13441 gctgcagaca actagcatcc aagagttttt ggtaccacca gacctcgatt tgaagatacg
    13501 aaacaataca gacatcacag cagtgatcat cacaaatgag gaccagtgca ttttgggcgt
    13561 agatgaccag atctgcatta tggtaaacac acaacggatc caaggcgagg gaattagggc
    13621 aatccaggaa aaggcaagac atgcaggcga ctcgttgatt gatgacatca atgagacctt
    13681 tgatctgaat gcaaagtttc actccatatt tgtccattat gaggacagtg caaatgaggc
    13741 actaaacacc acaggtgaaa tctctggtgc cggaacggta tctgctgtat atactgccca
    13801 aatgcagagc accgatttta tgttcaacaa gatttccgga gttttgattc caaggcaaat
    13861 tcgaggcatg ggtggatttt atgatgtagc ccaagagcta tcaaaagacg acagctcgag
    13921 gatgactttt actatttttc ccaagggtga agtctccatc atgcagttaa aggtctcaga
    13981 aaaatatccc aacattgcaa aagatgtaag catagttgat cccttgatgt ttctcaaagt
    14041 agaccaaata aaaaagtcca gttatttttc ggttgggttc tttccactca attcactcat
    14101 ccatgtcata gttttaccag agaaggagat aaatgaagtc catgttacaa atgtgattga
    14161 gccgacaatc aaggatgggc agaaaattcc atccgatctg tcaaagaacg gatggttctt
    14221 tgactccgaa tctggcgaaa aaatagatgc aatgtacctc tttggggaag gactttctgc
    14281 gaatcgtcag gacttaatgc tagtgattgg cgatgagcaa gaagaactgc agacaggatt
    14341 taatgagacc tacatcctaa ttggaatcgg cgccgcagca gcaggtgccg cagcgtttta
    14401 tctaaaagga atcaaaaaga aaaactagat tattaggact gctgccgcaa acacagtagt
    14461 ccacctgccg tctttgtcgc ctactgcagt ctgcgttacg ttcattgact tgtatatctg
    14521 gcctgagatc ttccactgct gccttttttc gtcccagctt ttgtcgatgt caaattttat
    14581 tccaagcgag gatgcaagca tttgtgcggc aatgtcttct gcatagtctc ctgccttctt
    14641 ttcattttgc ccaaatgcct catattctga gaggtagccg tatcgctttg tgtcagatgg
    14701 ctgtgcaatc ccgacagacg ccgaaatcag cctgtgtggc tcatctgtct ggtttcttga
    14761 ataaatggta aagagaacct gtcctgccct gatgagtttg agtccttctg cgcgcggaac
    14821 cagttttgcc ccgggcggaa atatgcttga aattagaaca atgtttgttc ctgagattcc
    14881 ggcatccctt agggcatact cgaaacttgt gaggcggtct tcgtggacgc ccttgccctt
    14941 tgtcaaaaat aatttttttg caactaagtc aagcaaattg tttttgtgaa aaaattctaa
    15001 ttatttatac cttttatttc agctctgcaa gtgtcctagc tatgtcctct ccgtgttttt
    15061 caacatcaac gtctcggaaa atttttctga ttatgccgtt tttgtcaatt acaaaagttg
    15121 agcgcttgga tagtcctgcc acattcagac cggcatatgt cttgcaggcg ttttttgcag
    15181 tatcactgag gtgcaggtac ggtatctggt attccgatac gaattttttt tggtcatctg
    15241 ccgtgtccac cgagattgca aaaagaaccg agcccgtctt tagaatatca gagtatgcag
    15301 agattacgct ttgggccatc ttgaagactt tttttgaggg gcacatgaaa agatggttct
    15361 ttggataaaa acaaaggact acgtttttgc ttccactaaa cgaggataat tttactgtgg
    15421 ttccatcact tgctggcagc tcaaagtccg gcgccgcatc accttcccgc atggatttag
    15481 tacgcagaac ttattattta ataaaatgtt gacagagtca tgctggattt ttcctttctt
    15541 ttcacactcg atagcatatt cagctcagaa ggattcatca taggatacta tgtctttacg
    15601 gtagccacat cacttattct aatcaaggag acaaaaaaaa gggtctttga tcttgccaag
    15661 ggaataaaat caatggtcta tgcgccaatc tcctttggca ttctaatttt gtatgtcgtg
    15721 tttgcgtatc cgctagtcga agaaattccc atcttaaatt ggagctggct tggctacaac
    15781 attgcgtttg gcccctttgc agaagaaggg ttctggggca tcgttccatt cattccaatt
    15841 ttgatctaca tgttcatcca catcaactat gtcgaggaat tttactttag gaagtcaaaa
    15901 aagatggttc tggtttgggc cgcagcacac attgccatgg gaatcaaggt gcacatggca
    15961 atactgctaa ttccgattgg gtttttgttc aagtacctct acgacaaaaa gggagtagaa
    16021 cactcgtatg caatgcattt tgcaacaaac atgctagttg ttaccgcgct tttttttacg
    16081 ctcgtcaggt gatggcttta cctgtgttag gaggcggtac ccaaagagcc ctccgaggca
    16141 caaattaatc actccgaaaa ctacgatgaa aaagacaaac ggcttgataa taaataatcc
    16201 agatacaatc ccccagatcc cacctgcaaa aaacattatc atgattatct tcagtttttt
    16261 tggtgggatg tacttcattt tagtggactg aatttgccac actattaaag attggcaaaa
    16321 actctcacaa cattttaaac ggctatattt tcttgtaaat tcgtgccaat atagctcagc
    16381 ctggcagagc atcggttttg taaaccgagg gtcaagggtc caaatccctt tattggcttt
    16441 ttactaaatt gctaaaaata ttcgatttgt gggcacgatt ttaagaggag ttgtagttcg
    16501 acgagaggat aatgcacaca aagagtcctt tgcccgagat cacctgtgct ctgtgtctca
    16561 ctccctttgg aatgaatatt gttgatgggg acgttacttt gtagatttca tcttcaagct
    16621 gtatctcgta ggtcagcttg tcattttctg agaggatgag gtttatttca tcgacgttgt
    16681 gtttgtgtaa tttgctgtat gccggtattt tttttggtag ggaatctata aaatgaactg
    16741 ccacatgcgt gtcggatttt tgtactagct ttttgctcag catcgataag cgcttgattg
    16801 gcgcttttcc gtggaatgga atattttcaa gtgattcttt tacacctttt acaattagtt
    16861 ttccgtatcg tttcacaaac taggttcatt gtttcactat aaattagtct ggattttcgg
    16921 ttctcaaaat tagattatcg ttataaacga taattaacaa taactagtgt gcagcaggat
    16981 agaacttcgg aatggtttgt tccaaaattc ggtccacgaa actttagagt aagcattgga
    17041 attttgtttt tgccatacac aagcattgtt acgtgttttg ccgcgtgggg tgcattatca
    17101 accagctttg agcttgaaag actagtagcc atatgtgcaa tttactttct tgcagtagga
    17161 gtatcagcac actgccttga tgcggtaggc ggtaaaacca aaccgtgggg cgagttgcca
    17221 aaaagaaaac tgtgggcaat tgcaaccaca tctcttgggg ttgcctttgt cattggtctt
    17281 tactatgcct tcttggactc gccgcttttg tttccaattg gaatagctga aggtttcttc
    17341 ttgtttgcgt ataacttgga attatttcgt gggaaattcc ataacaacgc atcaactgtt
    17401 ctttcgtggg gagtgctccc agtttttgca ggctcggcaa tccaaacaaa ctccataact
    17461 ctagaagcaa tactgctttc tgcagtatct ggaatcatca catatttgtt gataataacc
    17521 tcaagaagat acaaagagct caaaagagac tctcaagatc atattcagat taagaaaaag
    17581 gagctagtcc taaaattaat tttcattggc gtagtatgca gcactgcagc ctttgttatt
    17641 gtgagaacat atctctagtt tactcacatt aggtttttag atttcccgtc aaaatggcag
    17701 acgtactgca tgtaagatat tgttttgtaa cagcaaggcc atgttttttc atttcagatt
    17761 cacagtcatc aagccatctg ctggttttta tcagattggg aagctcagag aacgcatcac
    17821 tccagcttgg aaggaataaa ccagctaaac gcagcattac aaaatgcatc ttccagagtt
    17881 tctgcaccag tttgtttttt ggatagataa aatcatgcaa aatgatttta ccgccatcat
    17941 ttaggtgagc aaggcatgca gttaccagaa tgcccgcatc acagtatttt ggaatgtatg
    18001 aagaaaccac acagtcaaac ttggcagaca ggcttagatc ttcggcatct tgcagaacaa
    18061 atgaaatgtt ttggtatgat tttgaatttt tctttgccac atccagataa ttttccgata
    18121 tatccactcc aactattttt gcaaaaggaa actgttctgc aatttttctt gtcagtattc
    18181 ccgttccgca cgccaagtca agataggaat ctgcttttgg gatttgcctt aggatctctt
    18241 ttttccaata acgatctttt ccaaatgttg cccacgtagc tattttgtca tatgtggtag
    18301 ctgtgttgct gaaaaatttt ggtacgaggt tttttggtga ctcggctgcc aacagcaatt
    18361 attgagcgtc gagtaattta aagaggctca agatgccacg ttgtaatggt ttattagacg
    18421 cgccgcaagc cttcagatca tcaggctata atgacggaaa caagcaatga gctttttctc
    18481 tgtcattgct taggtaaaca aattgacaac attcgaacaa ctaggaatta aaaaatccat
    18541 cattgatggg ctaaaggaaa taggatttga ggcaccattc ccaatccaag aggcaactat
    18601 acccgttttg ctttccggaa gagatgttat aggccaggca cacaccggta ccggcaagac
    18661 tgcagcatat gccatttcga tgctgcaaaa catcgagcca acaaaaagaa tccaaggcct
    18721 gattttggca cccacaaggg agcttgcagt acaaatcaca acagaattca aaaagtttgc
    18781 aaaatacact ggagtcaaga tagtctcaat ttatggcggc caaggaattg gaatacaaca
    18841 ccaagcgcta aagcacggag ttgaaataat tgtcgcaacg cctggaagac taattgacca
    18901 cctcagacag gaatcaattg agctaaatga cgtaacgtac gtggtgcttg atgaggcaga
    18961 cacgatgctc gatatgggat tcattgacga tatcaagttc atcttggagc tgaccccagt
    19021 aaacaaggta atgtcattat tttcagctac tatgccacct gagattttgc gattagcaga
    19081 agaatatctt aacaatccaa aacagttttt gcttgatgcc gacgaccttt ccggcgaggg
    19141 aatagaccag tcgtttttgg taataaaaga cagagagaaa atgagttacc ttacacaatt
    19201 tatcaaagaa aacaaaaagg gccagacagt cgtgttttgc tcaactaaga acagaacccg
    19261 cgatgttgcg cgtgagctca agcacaacgg atttaatgca gttgcaatcg aaggcgacat
    19321 gtcccagcac agaagggaga tttcaatgtc gcagttcaga cgtggcggag cagacatact
    19381 agtagcaaca gatgtagctg caaggggaat tgacgtcccg caggtagcac tggttgtaaa
    19441 ctatgatgtt ccaaaccaag aattgatcta ctttcataga ataggaagga ctgcccgggc
    19501 aggtgcagag gggcgggcaa tcacattggt atcatattct tcaattggcg attttaatat
    19561 cataaagcag caaaccaaag tcaacatgac tgatcttaac gaaaagcttg gaatcgtcgt
    19621 aagtattcca gacccactaa aaagacaggt cggccagaga aggacgtttg ggcgcggagg
    19681 cggagacaga agacaaagtt atggacgtcc agatagcaga agaccaagct atggccgctc
    19741 cgataacaga aggcccagtt atggcaggtc tgatgacaga agaccgcaat atggccgctc
    19801 tgatgataga aaaccaagct atggtaggtc tgatgacaaa agaccgcaat atggccgctc
    19861 tgatgataga aaaccaagct atggtaggtc tgatgataga aaaccaagct atggtaggtc
    19921 tgatgataga aggcccagtt atggcaagtc cgagaatcgt ggcagctatg gccgctcaga
    19981 cgacagaaga actgaatatg gcaagtcaga taacagaaga ggtccgatgc gcaacgcagt
    20041 caggcgacca aaccaccgaa ggcgttggta agctttttta aaagtcggag ttaagaaaaa
    20101 acaatggaaa ttggttttgt tttactgaat tgtgacttgg gcgcagagga atatttactt
    20161 gaagaaataa agcaagttcc agaagtaaag caggcctatc ttacgtttgg agcatacgat
    20221 atcataattg agattcatgc caagtctgct gatgagtttg acaagaccgt ctcaagcaag
    20281 atacgccgac tatctcgggt catgagcacg atgactctaa aggtgctcag tacggcagaa
    20341 aagcactaat acaatttttt gaaggtaaga caaattggaa aaaatagagt ataccggaac
    20401 tgtctgggca ataaaccaca acaatccaga gcccctagta gaacactgtc taaagaagct
    20461 ccagcaatat ggagtaaaaa aagaagacat acgcctcact gatgcgccag aaaacgtaaa
    20521 gattggagaa attgtagtag agatatggcc gtatcatctg gatgttgcgc gagtcaggac
    20581 gattcgaaac gagtcattta tttccggctt ggtcatgcaa attgagctca aagcagacga
    20641 gcagggaaac tatatcgact aggtttgaaa aaacgaatca gtgtttctaa ttagataata
    20701 ccattcatgc aataatttat ggagttgatg agccgtcaga acgcggcgga gttacaaaga
    20761 aggtcatctc cttactcgat gccttgaatt tttcgccaga cacagtcaca tttagcacgt
    20821 atgccccagt ccttgaatta tccgggatca aaagacttgc ctcatagtag ccaaattttt
    20881 gcgtctctcc acttgaggtt tcaatcacat taccaagtgg gtcggttatt tttgcggaaa
    20941 ttggcactcc ttccaagtag cccgaggtct tgtaaaagtc agaaaaagaa tttagcagtg
    21001 gatcaaacgt cctcaccgta aacttgtata ggcccatcca ttcgacacgg tctacatgtc
    21061 tgactagtac taggacatct tctttttctg ctacggtctc tttttctggc aatattgcag
    21121 caaagtctac ggttttttgt tcctcaattg gctttaggat tgcaactaaa agaaacctct
    21181 ctgagacgcc atccctagtt gtagctccgc tcagctggta aatgctgccc tcctggtttg
    21241 ataccagcat ccttccgttt gccctaattg tcaagtcgtc agactcggca tttagcctaa
    21301 agagtcttcc gtcccgataa aatatcaagt taaaattttc aattgcacgt tccttatcat
    21361 cgagtacaag tgtgccttgc cgcaagactg ggtttgcagg actagtcatt tctagttgga
    21421 agtccagcgt catcttgttt gccgcatcat tggaaatttt gtatccactt gccgacagaa
    21481 aataatcggt ttcttgtgca tacgactgtg taaataagat tgcaagtgca aatattggaa
    21541 tagctattag tgatttcaac aagatttgat ctttttaatt gctattaaga tttgggcacg
    21601 agtcagtttt cccttttgtg aattttatgc aacaactggt taatatcgag cgtcagggtc
    21661 taataaaata atttgaaatc aacatacctt gtggttgggt ttggagtaat aattgtcgcc
    21721 attgcagtat tttattttta ttccgcagac caggcaaaaa tacgcgggtt taattttgga
    21781 aacagccttg atgcagtcca aggcgagcta aagcaggtcc agacggactt ttactccaaa
    21841 aaaaggctgc tcgatgaaaa cacaataaca aaagaagagt ttttggaatt ttctgaagaa
    21901 cacgtcgaaa aaatggagga aatggtatca aaatacgaaa gcctagtatc gcctaactct
    21961 tttgtaaagg cagtcgaact cttcaagaca tcaacgcaaa aacagcttga gagcgaccgc
    22021 tttctaatag aatggattag aacaaacgac acttcagaca gggtgcgctc agacctgctc
    22081 ttgcaggaat cgtttgaaaa cgagatggct gcactctcct cctttgagaa ggcaaaaaat
    22141 tcctcaggtc aggactagag aagccctttt tggtgcaaca agtccagcgt aggcgcaatt
    22201 gtctcaagga ttatcttttt gtctagtggt atctggtctg aaaacttgat tggaaatgta
    22261 aggctcagca tgtctgagtc ggtgtgcgac attcggattg catgcctcat ttggttttgc
    22321 gacaaaaact cgtcccacgc atcaagcctt gcgcttgcgt cggcaagctg gccctgcaga
    22381 tacacaatgt ccagatcatc atttgagtca aagattccaa attttaccat tggcactgtc
    22441 atgtacccgc caggaacctt gtcgccgttt ttgtgcatct gctcgattat ctcttctatt
    22501 ttttcttctg gctggatgtc accatattct ggtacaaagt atcctgctat tttttgatcc
    22561 ttatcaaaga ttctaaaaaa atcagcttcc cgttcgatct tccacatgca atgctagatt
    22621 ttgcgccaac tgttataaat catagattcg caacaaaaac gaattggcaa tcacaaagta
    22681 cacaaaagat tttcttgatg gtcttattga ttattacata agcgaggcgc catcatacaa
    22741 gcagattgcc gagtcatact ttcctgaaat cacctcagtt gaggacgcag cctttggaat
    22801 aattgccggc tgcatttatt cggcgttttt aagagcatac gaaagcgaaa acaagccagt
    22861 cgagcttgat gacatgcagg agttcaacaa gatcatcaaa gaaagggcag ccctcataaa
    22921 aaaggccatt ttggaataag aataaatccc aacacaaaaa ccacataaca atgggcgacc
    22981 taacccgcta cagaatccac caaatcgtag ataacggcca aattgtccag ctggcacttg
    23041 ccgaagacgt ccagacagag ccaatgtcgc aaaaacaact gatcatggac tctgttgcca
    23101 aaaagctaga ccccgaagta aaagaacagg tgcagcctct tttggaggca atactgcagg
    23161 cccagcccac aatcaagata aaaagctacg agcagactac catttctatt acgatgccgc
    23221 gtgcaagata tgaaagaatg ggaaggccac aagtgggcga agtcctagaa atcaacattt
    23281 caagaaagta ggttttctat ttctggtatg gttgccagag gcagtgagca gctaaagttc
    23341 ttgcatacaa atactgcggt cttggaatca tcgaattcct ttcctgcaaa aaacgcatgc
    23401 tttgatagct gttttagctg gtcggcgttt tgtattgtaa caagaattgt ctctgggagg
    23461 aatttctttg ttacaaagtc atagagttcc ttgttttttg tgttgattaa tgtgatttcc
    23521 gttggtgtct gcaggtaaag gtagattgtg tttagcaaaa acccaaatcc aaacgggttt
    23581 tctgctgcca tgatagcaaa cgactccatt attttagtgg atatctccaa gaagcttttt
    23641 tgctgcgtta gatgatacag tattaacagt gcaagtgctg caacggagtt cccagacggc
    23701 atggacaaat cataattgtt ctttggtctg attatcagct gttctgaatt gtctgcagta
    23761 aagtaaaagc tgttgtgttc tgcatcccag aaatgatcaa ctagataatt tgcaagattt
    23821 gtagcaagct ccagatattt tgcttgcggg tttgcctcaa agacgtcaat taatgcgttg
    23881 acaaaatagg catagtcttc aagatagcca tgtatcttgg ccttgccatc tttgtaggtg
    23941 cgcagtaact ggccttctgt gatcaagttt ttttctataa atgaaataca actctctgct
    24001 gccgcaaggt atgatttgtc atctgtgacc cgatatgcct ttgcaaatgc agatatcatc
    24061 aatgcattcc aggaagtcaa gaccttatca tctagacctg gtctaatcct catggctctc
    24121 gcatcaagta attttttttc accttcggaa actatctgcc tgactttgtc ttcagttatt
    24181 ccaaaatgaa atgcaacact tgaggtgcta atgttgttgt ttagtattgt atttccttca
    24241 aagtttccgc cgtccgtaac gtcatagtac aggaaaaaga cttcggcatc agcgccaaga
    24301 atatctcgga tctctttttt cttccagaca taatacttgc cctcctcgcc ttccgaatca
    24361 gcatcaagtg cggagtaaaa cccaccttct tttgaggcca tctcacgaag gacaaagcca
    24421 agcgtctttt ttatcacatc taggtaaaac ggatcctttg ttatttggta ggcctccgcg
    24481 taaacaattg gaagcagtgc gttgtcatac agcatttttt caaagtgtgg cacaagccat
    24541 cgagcgtcag ttgaatacct atggaatccc ccgccaacct gatcaaagat tccgcccctc
    24601 gccatcttag tcagtgtctt aagaacaaac tcggaaaatt ttgaaatttt ggaaattttt
    24661 gaatatcgca gcaaaaacga aaggtttgcc gcatttggga atttcggcgc gctgccaaat
    24721 ccaccatatg tgttgtctgc aatttggatt aggtttactg ctgcctcatc aagaattgtc
    24781 ttttcaagtt tggcaggagt cgtcacatgt tctgcctgtt gcaaggtagt catgaaactt
    24841 tcagctgcct ttttgacatc gcttggtttt tctttccaag cctgcgcaag ctgcctcaga
    24901 atgcttccaa agccgggcct gccatagctg tcaaggattg gaaagtatgt cccaacatag
    24961 aacggttttt ggtcaggcgt cagaaaaacg ctgagcggcc accctccctg gcctgttgac
    25021 atctggcata ccttttgata aatgtcgtct aggtctgggc gttcttccct gtccactttg
    25081 atgctgataa aattctcatt catgattttg gcaatctctt cgttctcaaa tgattcgtgt
    25141 tccatcacat gacaccaatg acacgagctg tatccgatgc tcagaaagat tggcttgttt
    25201 tcggtttttg ctttttgccg tgcctcctca caccatggat accagcccac agggttgtgt
    25261 gcatgctgca agagatacgg gctggtttca tgaatcagat ggttttgtgc catgtttttt
    25321 tacaatttta atggtatttt tgcctatgtc gagtttgtta aaaaagaacg ggttttgccg
    25381 tactcattga tacgccaaaa cgaattgttc cactgaactt ggagccaata ttggtttttt
    25441 gaaaaatgca aatatgtgta atattgttaa gaaaagctta gcatctttta agaatacacg
    25501 atattcgcac tttatcacga tatttgataa aactaatgtt taaaattgac atcatcttat
    25561 atatgatcac atta
//
LOCUS       DASWKH010000137         8078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_712534, whole genome shotgun sequence.
ACCESSION   DASWKH010000137 DASWKH010000000
VERSION     DASWKH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712534"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..658
                     /locus_tag="VGA92_09745"
     CDS             179..658
                     /locus_tag="VGA92_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9846722.1"
                     /translation="MNRMVLEILIAEDNEFTAKQYKIALEKRSHKVTLAKDGVECVDY
                     YTNEAKYEELFRKTRTPPFDLVLLDHDMPRMTGADAAKEILECRPNQRLIFLSAYGNN
                     IMQRLKDLRDETVQIIQKPFSLGYLINKIEGANIKNRIVNLQDEKLMTTSQDSAAIR"
     gene            735..1154
                     /locus_tag="VGA92_09750"
     CDS             735..1154
                     /locus_tag="VGA92_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846723.1"
                     /translation="MSQKVWLGGLYLKEEGGYDIVLRALNHYKKRLKSIGHSPELRDA
                     SMFAQIVQQEAMKTGPLIEPTINKIKNGLEDPTVLNELQSDIPLFEKALNCYYSDIQK
                     AKTNSDKFYSELVPDSNVAASDLVKIKIALAKISQFS"
     gene            complement(1213..2487)
                     /gene="prf1"
                     /locus_tag="VGA92_09755"
     CDS             complement(1213..2487)
                     /gene="prf1"
                     /locus_tag="VGA92_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HEX9846724.1"
                     /translation="MPKITIEKQDSVKIYKIKKILDELSRIQGRGTELISVYVPKGKQ
                     LHMVINNLREEQGTADNIKSDLTRTHVVDALSKVQQRLKLYKTTPERGLVIFCGALPP
                     EGGGPIGSEVVKVWEIEPPKDLPTFLYRCDDHFHVDILKDMLKDENLIGFLTIDTKDT
                     GWGLLYGNKIEVLRETASGVAGKHRQGGQSARRFERLREMELTYYYNRVAEVTKELFI
                     DIYPIKGLIVSGPGPTKEDFLKSNYLDYRLQNMVIATLDISYSGAEGIREAFVKAQDI
                     LSDFRLVEEKKLVEKLFREINSHSNLGVYGLKDILHLLKNNVVDTILITDDINLYRLE
                     VTCKRCKNVQEEIIERVKIIPRQTELLNKPCPSCKSTDQEANSQDLIDYLSLIATPVG
                     TKIEVISGISEHGAMLSSLGKMGAILRYNPNQ"
     gene            2602..2985
                     /locus_tag="VGA92_09760"
     CDS             2602..2985
                     /locus_tag="VGA92_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846725.1"
                     /translation="MGKQILPYCVPLEDYPKLLRYRVTLQGFYHIEDLVSEIYLEGKK
                     VPMHKLVSLGILLRVNMCQTMNDENESLYQDVSDKAKKFGGVEPTYLSECIQTLILRG
                     LLESNPKYDRQLALDLYRQSDLNTI"
     gene            complement(2971..3612)
                     /locus_tag="VGA92_09765"
     CDS             complement(2971..3612)
                     /locus_tag="VGA92_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012215264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9846726.1"
                     /translation="MAKYHIAIPIAIGIVAGIFAVTYFGNESKQGDFTKQDLVENGSP
                     YEGNPSAPITIIEFGDYQCTFCYRFHDSSLGIIKQEYLETGKANLVFRDFPLNGPDSV
                     LAAEASHCAKDQDKFWEYHDELYQNWGGERTGWVNRGSLAKFAQSVGLDLEEFNSCLD
                     SAKYRQQVLDAYDFGQEIGIEATPSFLIISGDKVVKITGNQPIDVFRKTLDGI"
     gene            complement(3650..4291)
                     /locus_tag="VGA92_09770"
     CDS             complement(3650..4291)
                     /locus_tag="VGA92_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4443 domain-containing protein"
                     /protein_id="HEX9846727.1"
                     /translation="MSTRHQVQILQNIVSRKGSSKVLTFSVPHIFKALQLLHKDQYVS
                     RASFCRHLHMGEGAVKTMIMHLKEESVADSTKSGTFLTNRGKNFIKNLLDVISAECAI
                     KKCVIAQGKFNYAVILKKYAFATKSGVEQRDYSILYGATGATTLLYRDEQFVFPNENI
                     DCLLDDLKTRNVLLSNLHPENGDMIIIASATDPFVAEIAAKNSALWTLATHNR"
     gene            4353..4568
                     /locus_tag="VGA92_09775"
     CDS             4353..4568
                     /locus_tag="VGA92_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HEX9846728.1"
                     /translation="MSHSKEIVISKSSIPKVALVVLAAIFVLGMFAVGYDQGHILSVV
                     FGEQAYADMYIHELTHDMRHAAGFPCH"
     gene            4578..5303
                     /locus_tag="VGA92_09780"
     CDS             4578..5303
                     /locus_tag="VGA92_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007551023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HEX9846729.1"
                     /translation="MKTALFIAIVLLSGCVAGVVHGLVNLAIVEPYLDTAIGIENQNL
                     FATGEEDDTPEFWAEYYSYRAWQKGGQLLAGAILGTSIGALFGIVFAYSRNVLPGNHF
                     VKKALVLALIMWTTLYIIPFAKYPANPPTVGDPETIILRQTLYLAFIAISGLGALGFY
                     QLYKRLDKKVLAFVGYAIFISAVFVAMPSNPDPITAPENLVNSFRATSAIAVSVFWLA
                     VGLVLGSLWQKLQPDRIKQSKFQ"
     gene            5406..5735
                     /locus_tag="VGA92_09785"
     CDS             5406..5735
                     /locus_tag="VGA92_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9846730.1"
                     /translation="MTRKFTLPQLKKYDITQKVIEALADAESRTILFSLIKQGRTASE
                     IADKYKIPLSSVYKKLSDLENLTLVHVERWMISDKGRKFKVYRSRISKADISIKKPEP
                     VLVLAPN"
     gene            5753..6079
                     /locus_tag="VGA92_09790"
     CDS             5753..6079
                     /locus_tag="VGA92_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014965157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX9846731.1"
                     /translation="MEKNSIFHLSHYDVTQQIIATLTNVYSRAVLFSITSKPKDAQTI
                     SDELKMSISTVYKILSNLEMLTLVYVDKFVISDAGKKIKFYKSRIKKAEIIVGGVTPT
                     LNLQSN"
     gene            6174..>8078
                     /locus_tag="VGA92_09795"
     CDS             6174..>8078
                     /locus_tag="VGA92_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX9846732.1"
                     /translation="MALGIASIILFMAQDSYAESQDAPLIHTTGEVVKIGMALTKSSI
                     IEGDLDGAKKYSKFTTDFYAHQINTLRQANIEFADDLHLSLIDIHAKIESNAEPNEIL
                     ADISSAEQYFDRFSKSEQIPYVISNMLATADETYQVAISKSDQENHIIATLLVDRAIQ
                     LFDSDASFDERQTGELQSFFSDLKIQMNEKKDFVSVGKLITTIQRDLTGTDSIIVDQS
                     NLYNVIRQMYDQTLSEIKSGNYEKAEELVIVAYLDNFEYLEADIEVVDADLLHTMEVN
                     MREELRTMVNEKKSFKEIEAFINDPILKDLDRAEGMVAEFLHPDIPVAQALTQPNKEF
                     QPMVSATAEQKTGVMGEIDFIRATLQTMLAQYQNGDYQAAYTSARTAYLDSYEHVEIP
                     LRAIDPDFTLEVELQFADLRSLINQKADYEKVEEATIKVRRSLDESERLVTGTGQLAP
                     TIAFTSSFAIVFREGLESVLILGAILTYLEASRNTRFKPYVYYGIIIAIGATAVTWFI
                     ASYVIEISGANRELIEAVAALSATAVLFYVSFWILNKIEHKKWMEFVKAKVWQASTTG
                     GTIVFVMLSFFTVYREGFETVLFYEAMFGFAKYMEMYVGLGFVVGLATLLGVYYITRK
                     LGKRLPLKMLF"
BASE COUNT         2464 a         1623 c         1610 g         2381 t
ORIGIN      
        1 gttgtagtgt ttttttaggc cgtcagtttc ctttgttgcc ctacccatgt gctttaccac
       61 aacataattt cacaaaagta cgatttgacc taaatggtgt ttaatcgaac acacattcct
      121 cctttgttcg tttaaaacaa aaaaacgagt cttaaaacgc ctttttcaag caagaaaaat
      181 gaatcgcatg gttttggaaa tactgattgc cgaagacaac gagtttacag caaaacaata
      241 caaaattgcc ctagaaaaac gcagtcacaa agtcacactg gcaaaggatg gcgtggaatg
      301 tgtagattac tatacaaatg aggcaaaata cgaagagttg tttagaaaaa ccagaacacc
      361 tccttttgat ctggtgcttt tggaccacga catgccaaga atgactggtg ctgatgcggc
      421 aaaagagatt ttggagtgcc gtcccaatca gagactgatc tttttatcag catatggaaa
      481 taacatcatg caaaggctaa aggatcttcg agatgagaca gtgcagataa ttcaaaaacc
      541 gttttcgctt ggctatttga taaacaaaat tgagggggcc aatatcaaaa acagaatagt
      601 aaacctccaa gacgaaaaac tgatgaccac ttctcaggat agtgccgcaa taagatagat
      661 tattgactca atctcgaaaa ttacagacta aatctacaat tattaaatat caatcgagta
      721 tatgatcaat caaattgtct caaaaagtgt ggcttggtgg attatatcta aaagaagaag
      781 gcggatatga cattgttttg cgtgcattaa accattataa aaaacgccta aaatccattg
      841 ggcactcacc tgaactaagg gacgcctcga tgtttgcaca aattgttcag caggaagcaa
      901 tgaaaactgg cccattaata gaacctacca tcaacaaaat aaaaaatgga ttggaagacc
      961 ccacggtctt aaatgaacta caatctgata tcccactttt tgaaaaagcg ttaaactgtt
     1021 attattctga catacaaaaa gccaaaacca attctgacaa attctattca gaacttgttc
     1081 cagacagtaa tgttgccgca tccgacctag taaagatcaa aatagcatta gcaaagattt
     1141 ctcaattttc ttaagagttc gactgaattt gttggaatca gtccagttta agaattaatt
     1201 tctaagaatc tattattgat ttgggttgta tcggaggatt gcccccattt tgcctaggct
     1261 gctaagcata gcaccgtgct ccgatatgcc tgatattacc tcaatttttg tgccaacagg
     1321 ggttgcaatg agggaaagat aatcaattag gtcttgagag tttgcctcct ggtccgtgct
     1381 tttacagctt ggacacggct tgtttaacaa ctcggtctgg cgaggaatta tttttactcg
     1441 ctctattatc tcttcttgga cgtttttgca tcttttgcat gtaacctcga ggcgataaag
     1501 attgatgtcg tctgtgatca gaattgtatc gactacattg ttttttagca aatgaaggat
     1561 gtcttttagg ccgtaaactc caaggtttga atgagagttg atttcacgga acaatttttc
     1621 cactaatttt ttttcttcca ctagtcgaaa gtccgataat atgtcttgcg ctttgacaaa
     1681 ggcttctcga attccctccg caccagaata tgatatgtca agtgtggcaa tgaccatgtt
     1741 ctgtaatcta taatcaagat agtttgattt taaaaaatcc tcttttgtcg ggcctggccc
     1801 tgaaacaatc agccccttta ttgggtaaat gtcgataaaa agctcctttg ttacttcggc
     1861 tactcgatta tagtaatacg taagctccat ttctcgcagc ctttcaaatc ttcgagcgga
     1921 ctgtcctccc tgcctgtgct ttccagcaac acccgatgcc gtctctctta atacctcgat
     1981 tttattccca tacagcaaac cccaaccagt atcctttgtg tcaattgtca aaaatccaat
     2041 caggttttca tcttttagca tgtcttttag aatatctacg tgaaaatgat catcgcacct
     2101 gtaaaggaat gtgggaagat cctttggcgg ttcaatctcc cagaccttga ctacctcgct
     2161 tccaatcggt cctcctcctt ctggtggaag cgcaccacaa aatattacca gaccgcgttc
     2221 cggcgtagtt ttgtaaagct tgagcctctg ctgtaccttg gataaggcat cgactacgtg
     2281 cgtccttgtt aggtcagact tgatgttatc agcagttccc tgctcttctc ttaggttgtt
     2341 tatgaccatg tgaagctgct tgccttttgg cacgtacact gaaatcagct cagttcctct
     2401 tccctgaatg cgtgaaagtt catcgagtat ttttttgatt ttgtaaatct taacagagtc
     2461 ttgcttttct atggtgattt ttggcatcaa ttgaacccct tgtggatgca atattaagat
     2521 tagttacttg attccaaaat ggaatgtatt tttatcaaaa aatgccgcac tagtaattaa
     2581 ttaaggaact gctgccgttt cgtgggaaaa caaattcttc catattgcgt tccgcttgag
     2641 gactatccaa agctgttaag atacagagta accctgcaag gattttatca catagaagat
     2701 ctcgtatctg aaatctatct tgaaggaaaa aaggttccca tgcacaaact ggtttcactt
     2761 gggatattat tgagagtcaa tatgtgtcag acaatgaatg atgaaaacga aagcctctat
     2821 caggatgtgt cggataaggc aaaaaaattt ggcggcgtag agccgacata tctgtcagag
     2881 tgtattcaaa ccctgatttt gcgaggattg ctagaatcaa atccaaaata cgacaggcag
     2941 ttggcacttg acctgtacag acaaagcgat ctaaatacca tctagcgtct ttctgaaaac
     3001 atcaattggt tggttgcccg ttattttgac aactttgtcc ccactgatta tcaaaaatga
     3061 cggtgttgcc tctattccaa tttcttgccc aaagtcataa gcatcaagca cctgctgcct
     3121 gtattttgca gagtctagac aagagttgaa ctcttctaaa tccaaaccta cgctttgagc
     3181 aaacttggca agtgagcctc tgttgaccca accggtccgc tcaccacccc aattttgata
     3241 cagttcgtcg tggtattccc agaatttatc ctggtctttt gcgcagtgtg atgcctctgc
     3301 tgccaaaaca gaatctgggc catttagcgg aaagtctctg aaaaccaggt ttgcctttcc
     3361 agtctccaaa tattcctgtt ttattatgcc taggcttgag tcatgaaatc tgtagcaaaa
     3421 cgtgcactgg taatcaccaa attctattat tgttattggg gcagaaggat ttccttcgta
     3481 tggagagccg ttttccacaa gatcctgttt tgtaaaatca ccctgttttg actcattacc
     3541 aaaatatgtc accgcgaata ttcctgcgac gattcctatc gctattggga ttgcaatgtg
     3601 atattttgcc acaaattagc acgagttaaa cgcttcaaat agtttactgc tatctgttgt
     3661 gtgttgcaag cgtccacagc gcagaatttt ttgccgcaat ttccgcaaca aacggatcag
     3721 tggcagacgc aattatgatc atatcgccgt tttcaggatg aaggttgctc aacaacacat
     3781 ttcgtgtttt caaatcatct aaaagacagt caatgttttc gtttgggaaa acaaactgtt
     3841 cgtctctgta aagcagagtg gttgcaccag ttgccccata cagtatggaa tagtctcttt
     3901 gttctacgcc agactttgta gcaaaggcgt atttttttag gatcacagca tagttgaact
     3961 tgccttgtgc aattacgcat tttttaattg cgcattctgc cgatataaca tcaagcaaat
     4021 tttttatgaa gtttttcccc ctgtttgtaa gaaacgtgcc ggattttgta gaatctgcca
     4081 cagactcttc ttttaggtgc atgatcatgg ttttgactgc tccttctccc atgtgcaggt
     4141 gtctgcaaaa acttgctctg ctgacatact gatccttgtg cagcagctgt aatgctttga
     4201 atatgtgggg gactgaaaaa gtaaggacct tacttgatcc ttttctagat actatgtttt
     4261 ggagaatctg gacttgatgc cgcgtgctca tgatcttaaa tattggatga attgtccaag
     4321 aatttaaata cattcagatg ggcacgacag acatgtctca ttcaaaagag atcgtaattt
     4381 caaaatcaag cattccaaag gttgcacttg tcgtactagc tgcaatcttt gtgttaggaa
     4441 tgtttgcagt cggatatgat cagggtcaca tcctaagcgt tgtctttggc gagcaagcat
     4501 atgccgacat gtacatacat gagctgactc acgacatgag gcatgcagca ggctttccct
     4561 gccactagtg ttccgtcatg aaaaccgctc tttttattgc aattgtattg ttatcgggtt
     4621 gtgttgccgg agtagtgcac ggccttgtca atttggcaat tgttgaaccg tatttggata
     4681 ctgcaatcgg aatagaaaac cagaatcttt ttgccacagg tgaagaagat gacacaccag
     4741 aattttgggc agaatactat tcttatcgtg catggcaaaa aggcggccag cttctagctg
     4801 gggcaatact tggaacatct attggagcat tatttggaat agtctttgca tactcgagaa
     4861 acgttcttcc aggcaatcac tttgtcaaaa aagcgctagt tcttgccctt ataatgtgga
     4921 ctacattata cataatacca tttgcaaaat atcctgcaaa ccccccgaca gttggagatc
     4981 cagagacaat aattctaagg cagacattgt atttggcatt tatcgcaatt tcagggcttg
     5041 gcgcacttgg tttttaccag ttgtacaaaa ggctagataa gaaagtcctg gcatttgtcg
     5101 ggtacgccat attcatatct gcagtttttg tagcaatgcc tagcaaccca gacccaatta
     5161 cggcgccaga aaacctagtt aacagcttta gggcaacttc tgcaattgca gtatctgtgt
     5221 tctggcttgc tgtaggattg gttttgggta gcctttggca aaaactgcag ccagacagga
     5281 ttaaacaatc taaatttcag tgatattgca caattttata gatagatgtg aaggtttaca
     5341 ttctcattct atgaaacaag aacaataaca ttttatttaa atgtcaaagt aatctagaca
     5401 actgattgac tcgaaaattt accttgccac aattaaaaaa atatgacatt actcaaaaag
     5461 taatagaggc attagcagat gcagaatcca gaacaatcct gttttctcta ataaagcagg
     5521 ggcgcactgc atctgaaatt gctgacaaat acaaaattcc actaagctca gtttataaaa
     5581 aattatcaga cctagaaaac ctgacccttg ttcatgttga aagatggatg atctcagata
     5641 agggaagaaa gttcaaagta tatcgaagca ggattagcaa agcagacatt agcatcaaaa
     5701 aacctgaacc ggttctggtt ctggcaccaa actagttttt gcagggattg gcatggaaaa
     5761 gaattccata tttcatctca gccattatga tgtcacccag caaataattg ctacactgac
     5821 taatgtttat tcaagagcag tattgttctc aattacctca aagccaaaag atgcacagac
     5881 aatatctgat gagcttaaaa tgtcaatttc aacagtttac aaaattcttt caaacttgga
     5941 aatgctcaca ctagtgtatg tagacaagtt tgtgatttct gatgccggaa aaaaaataaa
     6001 attttacaaa agtaggatca aaaaagctga aatcatagtc ggcggcgtta cgccaacctt
     6061 aaacctccag tcaaactagc agcctgctct cattctacaa tcttagaaca ttatccctgt
     6121 tttaaataca gttagctttt cctaataaaa tattgagcac gtggcattgg ttagtggcac
     6181 tgggcatagc atccatcatt ttattcatgg cacaagactc gtatgctgaa tcacaagacg
     6241 caccactaat ccacacaacc ggcgaagtcg taaagattgg gatggcactt acaaaatcaa
     6301 gcataattga aggagatttg gatggcgcaa aaaaatactc aaaatttacc accgattttt
     6361 atgcccacca gattaacaca ctcaggcagg caaatatcga atttgcagac gacttgcacc
     6421 tctcattaat cgacattcat gcaaaaattg aaagcaatgc ggaaccaaat gaaatactag
     6481 cggacatatc ttctgccgaa cagtattttg acagattctc aaaatctgaa cagattccat
     6541 atgtgatatc aaacatgctt gccactgcag acgaaaccta tcaggttgca atatcgaaaa
     6601 gtgaccaaga aaatcatatt attgcaacct tgcttgtaga tagagcaatc cagctttttg
     6661 actccgatgc gtcgtttgat gagaggcaga ctggagagct gcagtccttc ttttctgatt
     6721 taaagataca gatgaatgag aaaaaagatt ttgtatcggt tggaaagttg ataacgacaa
     6781 ttcagcgtga tctgactggt acagattcaa taattgttga tcaaagtaat ctatacaatg
     6841 taataagaca gatgtatgac cagacattat ctgaaataaa gagcggtaat tatgaaaaag
     6901 cagaagagct tgtaattgta gcttaccttg ataactttga gtatctggaa gcagacatcg
     6961 aagtagttga tgctgatttg ctgcatacaa tggaagtcaa catgagagaa gagcttagaa
     7021 caatggtcaa cgaaaaaaaa tcatttaagg aaattgaagc attcatcaat gatccaattc
     7081 taaaagacct tgaccgggct gaaggaatgg ttgcagaatt cctacatcca gacattccag
     7141 tagcgcaggc attaacacaa ccaaacaaag aatttcagcc aatggtctct gctaccgcag
     7201 agcaaaaaac aggcgtgatg ggcgaaatcg actttatccg tgcaacactt cagacaatgc
     7261 ttgcccaata ccagaatggc gattaccagg ctgcctatac gtctgcaaga accgcatatt
     7321 tggatagtta cgaacacgtg gagattccat tgcgtgcaat cgacccagat tttacattgg
     7381 aagtcgaatt acagtttgcc gatcttagaa gcttgatcaa ccaaaaagcc gattatgaaa
     7441 aggttgaaga agccacaatc aaggtgagga gaagtcttga cgaatctgaa agattggtta
     7501 ctggcactgg ccagcttgca ccaactatcg cgtttacatc atcgtttgca atagttttca
     7561 gagaaggttt agaatctgtt ctaatcttgg gtgcgattct tacctacctt gaggcctcgc
     7621 gaaacacacg gttcaaacca tatgtgtatt atggcataat tattgcaata ggtgcaacag
     7681 cagtaacttg gtttattgcg tcatatgtga tcgaaatctc tggtgcgaac agagaactca
     7741 ttgaggcagt agcagcactt tctgcaacag cagttttgtt ttacgtgagt ttttggattt
     7801 taaataaaat tgaacataaa aaatggatgg agtttgtaaa ggcaaaggtt tggcaggcat
     7861 ctactactgg gggcacaata gtgttcgtca tgttgtcatt ctttactgtg tatagagagg
     7921 gatttgagac cgtgttgttc tatgaagcga tgtttgggtt tgcaaaatac atggaaatgt
     7981 atgtgggtct cggatttgtt gtgggccttg ccacactact tggtgtttat tacataacaa
     8041 gaaaacttgg aaaaaggctc ccactaaaaa tgctattt
//
LOCUS       DASWKH010000138        10447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_340261, whole genome shotgun sequence.
ACCESSION   DASWKH010000138 DASWKH010000000
VERSION     DASWKH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340261"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VGA92_09800"
     CDS             <1..750
                     /locus_tag="VGA92_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX9846733.1"
                     /translation="KEFSSYVNRIKKGDFEISEFTRIAIKIDGKPVYPVINDVAVFPS
                     KSAMLMEHILRVNHEEVWHDSSDGVIISTPIGSSAYSMSAGGPMIFQDSPVFGIVSVN
                     SLDTTRRPLVVHDTSLIEIDEISSRLFCEVVLDGVDRFRINNMLECTKSDASAKIIKM
                     KKDSSAVSALAKKVKLAEDLLNMPPSSKLILKTLEYEGIMTQKELAAKTLLPDRTVRL
                     ALRHLLDKGYVKKKISIRDARQKIYEISKMD"
     gene            807..1109
                     /locus_tag="VGA92_09805"
     CDS             807..1109
                     /locus_tag="VGA92_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846734.1"
                     /translation="MSAVIIIPITIVAIIGLATYFIYRFVVYDFLCKQTVVKTLQKYN
                     IKETPSQIIKEYHSSKGENISEKEAQRLEKHYRQHEPEQFLAMYDTIRDGSKTNAS"
     gene            complement(1122..1985)
                     /locus_tag="VGA92_09810"
     CDS             complement(1122..1985)
                     /locus_tag="VGA92_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX9846735.1"
                     /translation="MSKYESPKMNINIGAAKAVLIGIVVLVIIGVVVSASVKIVDAGH
                     RGVLLYWSAVDTSVPPLQEGLHFVTPFANEVVNIEVRTLKFERSTTSASRDLQTVSTT
                     VTVNYRPSPESIHHLYQELGLDYQNRVIQPAIDETVKQVTARYNAEELITKRPLVKTD
                     IEESIRERLNDFNVITDVISITDFQFSALFAQAIEAKVEAEQRALQAENDLRRIQVEA
                     RQQEAKAEGIAKANIAEAEGESEAIRIINEALANNPNYLEWLKTQRWDGRLPLVVGEG
                     GTPFIQIPVTP"
     gene            2129..2530
                     /locus_tag="VGA92_09815"
     CDS             2129..2530
                     /locus_tag="VGA92_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846736.1"
                     /translation="MDSNFTWLYLLIFLIPLTRVIPRIIRQRRMKKDSTNQTFQKERF
                     ESSLHESIQKSPESARPQTKDMLVLGELYRGARTFESIKKNTGFSNDELNSILEDLEK
                     QDMMRVEQKNGILGSKIEIYATEKGFKKYLS"
     gene            complement(2647..4245)
                     /locus_tag="VGA92_09820"
     CDS             complement(2647..4245)
                     /locus_tag="VGA92_09820"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9846737.1"
                     /translation="MQTKFIFVTGGVMSGLGKGVVTSSIAKLLQLSNQKVSCVKIDPY
                     LNYDAGTMNPVAHGEVFVTDDGGECDMDIGNYERFLNQNIPKTHNITTAQIYSAVIDA
                     ERRGEYLGACVQIIPHVTDEIKLRIRAIAENEKLDVLVVECGGTVGDIESLPFLEALR
                     QLRLEEGPQNVIFVHVTLAPSLDVVGEQKTKPTQHSVQELRRIGIQPDLMAVRCSTPL
                     NESTKKKISMFTNVTTRDVFSCHDVKSIFMVPQILYDQGIVNTIFTKFGKVGLVNASA
                     NWDKWNAIVNSLESGSGEVKIAMVGKYVTLADSYVSVNQALKHAGAKLGKSVVIDWID
                     SEAINGGIEKLSKYNGIIVPGGFGVRGADGIIGAANYAREKNIPYLGICFGFQLAAVA
                     FGRHVCNFNDANSTEIDPNTTSPVVDLLPEQKTVSNMGGSLRLGAHEIIVKNNTLAYK
                     TYNANKVMRRHRHRYEINKKYLDTFEKNGMIFSAESDGSKRMEILEIPSHKFYFGVQF
                     HPEFNSRPGFPEESFQAFVNAASG"
     gene            4330..4599
                     /locus_tag="VGA92_09825"
     CDS             4330..4599
                     /locus_tag="VGA92_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX9846738.1"
                     /translation="MLFEGIWLTLVGSIASVLVSSSFIPQIIKGYRTKRLNDVSYLLM
                     ILISIGMSLWIVYGIEKQDMVIVGANTATIALNMILLGMKVRYAK"
     gene            complement(4603..5421)
                     /gene="trpA"
                     /locus_tag="VGA92_09830"
     CDS             complement(4603..5421)
                     /gene="trpA"
                     /locus_tag="VGA92_09830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9846739.1"
                     /translation="MSKETSRIKAKFAKLASKKEKALITYIMTGYPSQKFTLSTIRAL
                     IKGGADIIELGFPFTDPLADGPVIQNASTTSLEKGTKLDQLFEIVRKIRKESDIPLVL
                     MTYTNILYHRGYDKFFKLAKSAGIDGLITPDMTVEESKEYIKAAKKHKIDTIFLVSPN
                     TSKPRLQKIVRQTSGFLYLVSVYGTTGMQTKIQQYTINALKNTKKIANSKIPVGIGFG
                     VSTPNDVRYYSKNGADAVIIGSALIKLIEKTPATKLEKTVIAFTRKLKAATRPI"
     gene            complement(5402..6592)
                     /gene="trpB"
                     /locus_tag="VGA92_09835"
     CDS             complement(5402..6592)
                     /gene="trpB"
                     /locus_tag="VGA92_09835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9846740.1"
                     /translation="MKIKFPKDGRFGEFGGKYIPETLVPAIEELEQNYLKYKNDPSFK
                     KELNYYLTEYAGRPTPLYYAKNLSEKIGGAKILLKREDLLHGGAHKINNTLGQALLAK
                     RMKKARIIAETGAGQHGVATAMACACLGLKSEVYMGYKDTIRQKLNVFRMNLLGCQVH
                     AVKSGSQTLKDAINEAIRDWITNVKDTYYLLGSAVGPHPYPVMVRDFQSVIGKEIMQQ
                     MKKFGKNNPDTIIACVGGGSNAIGSFYPLIDTDTEMIGVEAAGKGLDTEYHSATLNVG
                     SKGVLHGMMTYLLQTKEGQIKETHSISAGLDYPGVGPEHAYLKDERRVKYASATDKEV
                     IDAFLLLTRTEGIIPALESAHAVAEAIKIAKTRSKSESIVVTLSGRGDKDVEVVEEYV
                     KRNF"
     gene            complement(6589..7362)
                     /locus_tag="VGA92_09840"
     CDS             complement(6589..7362)
                     /locus_tag="VGA92_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEX9846741.1"
                     /translation="MTQTLRRLVENSHKAISEGVYEISESLQKSPFDLIESIKSNSHA
                     SLITEVKFASPSLGQIRQHSDPVEIAKSMVRGGALALSVLTQPHLFNGSPTYFMEIRK
                     QLKVPMLMKDIVVDKIQIDAAKKIGADYMLLIQSLFDSGHLKEIDEFIGYGHRNGLKV
                     LVETHTKEEFENSLKTSADLVGINNRNLDTLEIDINTTKKLLEGHTKNRLIVSESGIE
                     SPQDIVFLKRCGADAFLVGSSIMKSSNIEENVRNLVHAI"
     gene            complement(7359..8405)
                     /gene="trpD"
                     /locus_tag="VGA92_09845"
     CDS             complement(7359..8405)
                     /gene="trpD"
                     /locus_tag="VGA92_09845"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9846742.1"
                     /translation="MIVNLTAKLKDGRNLTINEMGDAMNEVLSDRISDEKKADFLRYL
                     SKKGESDDELYAMLGKMDEYGLHIMPKCQGTIIDVCGTGGDKLQTFNISTTASFVISA
                     VGGFVAKHGNRSVSGVSGSADIFEYFGYDLNATPEKVTKIIEKFKIGFLFAQRFHPAM
                     KNVANARKILGTRTAFNLLGPLSNPANVKNQLIGVFSDEYLQRIILILKRRGSENIMT
                     VHSQDGLDELSTTAKNNVCFLHDGKMEKMIIDSEKLGLHKSAITDLQVSTRQEAIKAF
                     LSVLNGTSNRSMQEITVLNAAGGLIVANIAKNFDEGVEMARQTIKNGKAYSHFVNFVK
                     ENGDFQKIKEVDES"
     gene            complement(8402..8989)
                     /locus_tag="VGA92_09850"
     CDS             complement(8402..8989)
                     /locus_tag="VGA92_09850"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX9846743.1"
                     /translation="MKFLIIDNYDSFVYNIAQILGELGVSSDVIRNDKITLDQIIKNK
                     YDAIVISPGPGTPEDKKYFGICSDVIQKLGPTTPILGVCLGHQGIIHEFGGKVVNAGC
                     VRHGKTSPIKHTNDSLFAGVKNPFLATRYHSLVGDKTIIPDVLEITAVAEDDGEVMAV
                     RHKQYPIEGVQFHPESILTTEGKKILANFVKSVKK"
     gene            complement(8986..10338)
                     /locus_tag="VGA92_09855"
     CDS             complement(8986..10338)
                     /locus_tag="VGA92_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX9846744.1"
                     /translation="MAIFGNSNPTVIPLDLPGSQFEIYSKLEQNYSHTFLFESLTGPE
                     ELAETSIIGFDPKIILQGYEDRVVLQYKDGTTKTIRTQDPVAELKNFVRQTNDTSFRY
                     LGGAVGIINYDAIKLYENIPIQNDKAKPLMEFGIYDDGILFDNKAKKFLYFYYDENRI
                     DKIKLADRAFGKIQLFEISTNMDESKFSEIVNQAKKYIYAGDIFQVVLSRKFSFSATG
                     DYLRVYKELRALNPSPYLYHMKFGQKTYIGCSPEMLIRVTGKEVETFPIAGTRKITED
                     EQKNEQLRQELINDEKELAEHTMLVDLGRNDIGRVCKYGSVHVTDLMQVKRFSHVQHI
                     VTHVVGTMDEKYDMFQAFEAVFPAGTVSGAPKVRAMEIINELEPDVRYQYAGAVGYFS
                     NNGCCDFAIAIRSIFFDGKNGFLQSGAGIVSDSTPQGEFKETEHKAGAMIAALKEAAK
                     "
BASE COUNT         2740 a         2173 c         2101 g         3433 t
ORIGIN      
        1 aaggagtttt cgtcatatgt aaacagaata aaaaaaggtg attttgaaat aagtgaattt
       61 acaagaattg caatcaaaat agatggcaag ccagtctatc ctgtcataaa tgatgtagct
      121 gttttcccat caaaaagcgc tatgctcatg gagcacattt tacgggtaaa tcatgaggag
      181 gtgtggcatg acagcagtga tggtgtgata atatccacac caattggctc ttcggcatat
      241 tcgatgtctg caggtggtcc aatgatcttc caagattcac ctgtttttgg tattgtgtcg
      301 gtgaactcgc ttgacactac aagacggcca cttgttgtcc acgacacaag tctgattgag
      361 attgatgaga tttcttcaag actgttttgc gaagtagtac ttgacggcgt ggacagattc
      421 cgaattaata acatgcttga atgcacaaaa tccgatgctt ctgcaaagat aataaaaatg
      481 aaaaaagatt ctagtgcagt ttccgcactt gcaaaaaaag tcaaacttgc agaagacctt
      541 ctaaacatgc ctccaagctc aaaacttatt cttaagacct tggaatatga gggcatcatg
      601 acacaaaaag agcttgccgc aaaaaccctt cttcccgata gaaccgtacg gcttgcactg
      661 cgtcatctcc ttgataaggg ctatgtcaaa aagaagatct caattcgtga cgcgcggcag
      721 aaaatttacg aaatatccaa aatggattaa attatgtgac tggtagtaga acaacaaaga
      781 aatactaaga aaatcaataa tatgtaatgt cggctgtgat aatcattccg ataaccatcg
      841 tggcaataat tggtcttgca acatatttta tctatagatt tgtagtctac gattttttat
      901 gcaagcagac agttgttaaa acacttcaaa aatacaacat aaaggagacg ccttctcaga
      961 tcataaaaga ataccacagt agcaaaggag aaaacatatc agaaaaagaa gcccagaggc
     1021 ttgaaaaaca ttacaggcaa catgagccag aacaatttct tgcaatgtat gataccataa
     1081 gagatggttc aaaaactaat gcaagttagc ggtttgcaaa ctcagggtgt aacggggatc
     1141 tggatgaatg gcgttccacc ctcacccact acaagaggta atcgtccatc ccatctttga
     1201 gtttttaacc actccaaata gtttgggtta tttgccaacg cttcattgat aatcctgata
     1261 gcttcggact caccttcagc ttcggcaatg tttgcttttg caatgccttc ggccttggct
     1321 tcctgttgtc gtgcctccac ttgaattctt cttagatcat tttcggcctg aagcgccctt
     1381 tgttcggcct ctacctttgc ttctattgct tgtgcaaata atgctgaaaa ctgaaagtcg
     1441 gtaatggata tgacatcggt tatgacgtta aaatcgttca gtctttctct aattgattct
     1501 tcaatgtctg tttttactag tggtcttttt gtaatcagtt cttcggcatt gtatcttgca
     1561 gtcacctgtt ttacggtttc atcaatggct ggttgaatta ccctattttg ataatcaagg
     1621 cccagttcct ggtagaggtg gtgaatggat tctggacttg ggcgatagtt tactgtaacg
     1681 gtcgtagata ctgtctggag atctcttgat gctgatgttg tcgatctttc aaattttagt
     1741 gttctaactt cgatattgac tacttcgtta gcaaacggcg tcacaaaatg aagtccttct
     1801 tgaagcggtg gaacgctggt atcgacggca ctccaatata gcaacacacc tctgtgacca
     1861 gcatcgacta tttttacaga agcgcttact acaactccta tgataacaag tacgactatg
     1921 ccaattagaa ctgctttggc tgcaccaata ttgatgttca tttttggaga ttcatatttt
     1981 gacaactgcg taattaatcg gaatccaccc taattatatc aaccgatgag ttgcaaatca
     2041 ttttttgtat ttgaaatagt aaaaattttg taaattatga ttagttttgc ccaacagatt
     2101 tattatatta tcaatcaatt aaagcaaaat ggatagcaat tttacatggc tttatctgtt
     2161 gatctttttg atcccattaa cacgagtaat cccacgaatc ataagacaac gaagaatgaa
     2221 aaaggatagt acaaatcaaa cattccaaaa ggaacgattt gaatcaagtt tgcatgaatc
     2281 catacaaaaa tccccagagt cagcaagacc gcaaacaaaa gatatgcttg ttttggggga
     2341 gctctataga ggagcaagaa catttgagag cattaaaaaa aatacggggt ttagcaacga
     2401 tgagctaaac tcaatcttgg aggatttgga aaaacaagat atgatgagag tggaacaaaa
     2461 aaacggcatt ttaggctcca agatagagat atacgccact gaaaaaggct ttaagaaata
     2521 tttgtcgtaa tgataaattt cgtatatgct actaagattt cgtatgttta agataagtat
     2581 ctaaattaat tgataatttt tgtgttaaat accgcaagtg aagagttaca aagatttttg
     2641 catgcactag cctgaagctg cattcacaaa tgcctgaaaa gactcttcag gaaaacctgg
     2701 cctgctgtta aattcaggat ggaactgcac cccaaagtaa aatttgtgag atggaatctc
     2761 caatatttcc atcctctttg agccgtcgct ttctgctgaa aagatcatcc catttttttc
     2821 aaatgtgtct aggtattttt tgttaatttc gtatctatgt ctgtgtcttc gcatgacttt
     2881 gtttgcattg tatgttttgt atgcaagggt gttgtttttg acaattattt catgagctcc
     2941 tagccgcagt gagcccccca tgtttgaaac ggttttttgc tcaggaagca aatcaacaac
     3001 tggacttgta gtgtttgggt caatctctgt agagtttgcg tcattgaaat tacagacatg
     3061 gcggccaaat gcaactgcgg caagctgaaa tccaaaacaa attccaagat atggaatgtt
     3121 tttctctctg gcatagtttg ccgcaccaat tatgccatct gctcctctca caccaaagcc
     3181 gcctggaaca attatgccat tgtattttga cagcttttca atgccgccgt tgattgcctc
     3241 agagtcaatc cagtcaatta cgactgattt tccaagtttt gcgcctgcgt gttttagtgc
     3301 ctggttgacg ctaacatagc tgtccgcaag cgtgacatat tttccaacca tggcaatctt
     3361 gacttcccca ctaccgctct caagcgaatt aactatggca ttccatttgt cccagttggc
     3421 agatgcgtta acaaggccga ctttgccgaa ctttgtaaat attgtattga caattccctg
     3481 atcataaaga atctgcggca ccatgaaaat cgatttgacg tcatggcagg aaaacacgtc
     3541 ccgtgttgtt acatttgtga acatggaaat cttctttttc gttgattcgt ttaacggagt
     3601 ggaacaccgt actgccatca aatccggctg aataccaatt cttcgcagct cttggacact
     3661 gtgttgggtc ggtttggttt tttgttcgcc tacaacatca agtgatggtg ccaatgtcac
     3721 atgtacaaaa atgacatttt gcggcccttc ctcaagcctt agctgtctta atgcctccaa
     3781 aaacggcaga ctttcaatgt caccaacggt tccaccacat tctactacca aaacatccag
     3841 tttttcgttt tcagctatgg ctctaattct gagttttatc tcatcagtaa catggggaat
     3901 tatctgaaca catgccccta gatactcacc acgtcgctct gcatcaatta ctgcagaata
     3961 gatttgagca gttgtgatgt tgtgggtttt tggtatgttt tggttcaaaa accgctcata
     4021 gtttccaata tccatatcgc attccccgcc gtcatctgtg acaaacacct cgccgtgggc
     4081 aacggggttc atggtaccgg catcatagtt taggtatggg tcaatcttta cacaggaaac
     4141 tttttggttt gataactgta atagtttagc aatagatgag gtcacgacgc ctttgccgag
     4201 tccagacatc acaccaccag taacaaaaat gaattttgtc tgcacattta cggatcaaaa
     4261 ttggtatttt taattcttta ggcacccatg cacaaaattt taatccatac tgatattggg
     4321 tgaatacaat tgcttttcga gggaatatgg cttactttgg ttggtagtat agccagcgta
     4381 ttagtatcgt caagctttat cccccaaata ataaagggat acaggacaaa gcgcctaaac
     4441 gacgtttcct atctgctcat gatactaatt tccataggga tgtcgctttg gatcgtttac
     4501 ggcatagaaa aacaagacat ggtgatcgtt ggcgcaaaca ctgctacaat agcgctgaat
     4561 atgattttac ttggcatgaa agtaaggtat gcaaaataaa tcctagatgg gtcttgttgc
     4621 ggcctttagt tttcttgtaa atgctattac tgttttttcc agttttgttg cgggggtttt
     4681 ttcaattaat tttatcagcg cacttccaat aatgactgcg tctgcaccgt ttttactata
     4741 gtatctaaca tcatttggtg tcgatactcc aaagccaatc ccaactggaa ttttggaatt
     4801 tgcgattttt ttggtattct ttaaggcatt aattgtgtat tgttgaatct tagtttgcat
     4861 gccagttgtc ccataaactg aaaccaaata aagaaagccg ctagtctgtc tgacgatttt
     4921 ttgcagcctt ggcttgctgg tgtttgggga gacaagaaag attgtatcga ttttgtgttt
     4981 ttttgcagcc ttgatgtatt cctttgactc ctccacagtc atatctggag taattaggcc
     5041 gtcgattcca gcactctttg ccagcttgaa gaacttgtca tatccacgat gatacaaaat
     5101 gtttgtgtat gtcatcaata cgaggggaat gtcactttct ttgcgaatct ttcttacaat
     5161 ctcaaagagt tggtctagct ttgttccttt ttctaatgat gtggtgcttg cgttctgaat
     5221 cactgggccg tctgctagtg gatcggtgaa aggaaatcca agctcaataa tgtctgcccc
     5281 gccttttatc agcgccctta ttgtagaaag cgtgaatttt tggcttggat agccagtcat
     5341 aatgtatgtt atcagcgctt tttctttttt tgatgcgagc ttggcgaact ttgccttgat
     5401 tctagaagtt tcttttgaca tattcctcaa ctacttcgac atctttgtct cccctgccgg
     5461 aaagagttac aacaatggat tcagattttg atcttgtttt tgcaatcttg attgcttctg
     5521 caactgcatg tgctgactct agtgcaggta tgatgccctc cgtccttgta aggagtaaaa
     5581 atgcgtcaat tacttctttg tctgtggcac tggcatactt gacacgtctc tcatccttta
     5641 gatatgcgtg ctctggtccc accccaggat agtcaaggcc tgcagagatg ctgtgggttt
     5701 ctttgatctg tccttctttg gtttgcaata ggtatgtcat cattccgtgg agtactccct
     5761 ttgagccgac atttagcgtt gcggagtgat attcagtgtc tagtcctttt ccagctgctt
     5821 cgactccaat catttctgtg tcagtatcaa ttaaggggta aaacgatcca attgcgttag
     5881 agccgccacc aacgcatgcg attattgtat cggggttgtt cttaccaaat tttttcatct
     5941 gctgcatgat ctctttacca attactgatt gaaagtcacg gaccattact ggatatggat
     6001 gagggcctac tgcagaacct aaaagatagt aggtgtcctt tacgtttgta atccagtccc
     6061 gtattgcctc atttattgca tctttgagtg tctgagagcc gctttttact gcgtgaactt
     6121 ggcagcccaa aagattcatc ctaaagacgt taagcttttg ccggattgtg tctttgtatc
     6181 ccatgtatac ttcagatttg aggccaaggc atgcgcaagc catggcagtt gccacaccgt
     6241 gctggccggc gccagtttct gcgataattc gtgccttctt catgcgtttt gccaaaagtg
     6301 cctggcccaa agtgttgttg atcttgtggg caccgccgtg taggagatct tctcgcttta
     6361 gtaagatttt tgccccgcca attttttcag agaggttttt tgcatagtat aacggagtgg
     6421 gtcggcctgc atattctgtg agatagtaat ttagctcttt tttgaacgag ggatcgtttt
     6481 tgtattttag atagttttgc tcaagttcct ctattgcggg aacaagtgtt tctgggatgt
     6541 attttccacc gaactcgcca aaccttccat cctttgggaa ttttattttc atattgcgtg
     6601 caccagattc ctgacgtttt cttctatgtt tgaacttttc atgatgcttg atccaaccaa
     6661 aaatgcatcg gcgccacatc tttttaagaa tacaatgtct tgtggcgact ctatcccgct
     6721 ctctgagaca atcaatctgt tttttgtgtg tccctctagc agttttttag tggtgttgat
     6781 gtcgatttcc agcgtgtcta aattacgatt gttaatccca actaggtctg cgcttgtttt
     6841 tagagaattt tcaaactctt cttttgtatg agtctcaact aaaaccttta atccatttct
     6901 gtgaccatat cctataaact cgtcaatttc tttaaggtgg cccgaatcaa acaacgattg
     6961 tatcagtaac atatagtctg caccaatctt ttttgccgca tcgatttgta ttttgtctac
     7021 tacaatatct ttcattaaca ttggaacttt gagctgcttt ctaatctcca taaaatatgt
     7081 cggtgagccg ttgaataggt gtggttgagt gagaactgag agggccaggg ccccgccacg
     7141 aaccatggat ttggcaatct ctactgggtc tgaatgttgc ctgatttggc caagtgaggg
     7201 tgaggcaaac ttgacttcgg tgattagtga ggcgtggctg tttgacttta tggattctat
     7261 taggtcaaag ggagatttct gtaaggactc ggaaatctcg tatactccct cgctgatcgc
     7321 cttgtgcgag ttttctacta gtcttcttag tgtttgggtc atgattcgtc aacttccttg
     7381 attttttgga aatccccgtt ttcttttacg aaattaacaa aatgtgagta cgcctttccg
     7441 tttttgattg tctggcgtgc catctctaca ccctcatcga aattttttgc aatgtttgca
     7501 acaattagtc cccccgcagc atttagtacg gtgatttctt gcattgagcg attcgaagtt
     7561 ccatttagta ctgataaaaa ggccttgatt gcttcttgcc tcgttgatac ttgaagatcc
     7621 gtaattgccg atttgtgcag tcccagcttt tctgaatcaa ttatcatttt ttccattttg
     7681 ccgtcgtgca gaaaacaaac attgtttttt gcagtagtgg atagttcgtc gagtccgtcc
     7741 tgtgaatgaa cagtcataat gttttctgat ccacgtcttt tcaaaatcaa aataattctt
     7801 tgcaggtatt cgtctgagaa gaccccaatt agttggtttt ttacatttgc gggatttgat
     7861 agtggcccaa gcaaattaaa ggctgttctg gtgccaagaa tctttcttgc atttgcaacg
     7921 tttttcatcg caggatggaa tctttgtgca aacaaaaagc caatcttgaa cttttcaatt
     7981 atttttgtca ccttttctgg cgttgcattt aggtcatatc caaaatactc aaaaatatcg
     8041 gcactgcctg atactccgga aacagaacgg tttccatgtt ttgctacaaa tccacctact
     8101 gcagatatca caaaagatgc agtagtcgat atgttgaagg tttgcagttt gtctcctcct
     8161 gttccgcaga catcgatgat tgttccctgg cattttggca taatgtgcag gccgtactcg
     8221 tccatcttgc caagcatagc gtagagttca tcgtctgact cacctttttt actcaaatag
     8281 cgcagaaaat ccgctttttt ctcatcagaa atgcgatctg ataagacctc gttcatggca
     8341 tcgcccatct catttattgt caagttccga ccatccttga gcttagcggt tagatttaca
     8401 atcatttttt tacactcttt acaaagttcg ctagaatttt ttttccctca gttgttagaa
     8461 ttgactctgg gtgaaactgg actccttcta ttggatactg cttgtgtcgc actgccatta
     8521 cctcaccgtc gtcttcggca actgcagtaa tctctaagac atcgggaatt atggttttat
     8581 cgcctactag tgagtggtat cgggtggcca gaaatggatt tttgacccca gcaaataatg
     8641 aatcgttagt gtgcttaatt gggcttgttt ttccatgtct gacacatccg gcatttacaa
     8701 ccttgcctcc aaactcatga attattcctt gatggccaag acaaactcca agtattggcg
     8761 tggtaggtcc gagtttttgg atcacatctg agcaaatgcc aaaatacttt ttgtcttctg
     8821 gtgtaccagg gcctggggag atcactatag cgtcgtactt gtttttgatt atctggtcta
     8881 gcgttatctt gtcatttcta atcacatccg aggagacgcc aagctcgcct aggatttggg
     8941 caatgttgta aacaaacgaa tcataattat caattattag aaatttcatt ttgctgcctc
     9001 ctttagtgcc gcaatcattg caccggcctt gtgttcggtt tctttgaact ctccttgcgg
     9061 tgttgaatca gagacaattc cggcgccgct ttgcaaaaac ccgttcttac catcaaaaaa
     9121 tatacttcgt attgctattg caaaatcaca gcaaccgttg ttcgaaaaat acccaacagc
     9181 cccagcatac tggtaacgca cgtctggctc tagctcgttg attatttcca ttgccctgac
     9241 ctttggagcg ccactcacag ttcctgcagg aaacacggcc tcaaatgctt gaaacatatc
     9301 atatttttcg tccatcgtgc ctacgacatg agttacaatg tgctggacgt gtgagaatcg
     9361 cttgacttgc atgaggtcgg tgacatgtac gctgccgtac ttacaaaccc tgcctatgtc
     9421 gtttcttccc aaatctacta gcattgtatg ttctgccaac tctttttcat cattaatgag
     9481 ctcttgtctt agctgctcgt ttttttgctc gtcttctgta atttttctcg tacctgcaat
     9541 tggaaatgtc tcaacctcct ttccagttac tcggatgagc atttcggggc tgcatccaat
     9601 gtaggttttt tgcccaaatt tcatatggta aagatatggt gatgggttga gcgctcgcag
     9661 ttctttgtat acgcgaagat aatcaccggt tgcagaaaag ctgaactttc ttgagagtac
     9721 gacttggaat atatctccgg cgtagatgta tttttttgcc tgattgacaa tttcggaaaa
     9781 ctttgactca tccatgtttg tagatatctc gaagagttga attttgccaa aagccctgtc
     9841 agctagtttt attttgtcga tcctattttc gtcatagtaa aaatacagga atttttttgc
     9901 cttgttatca aataatatgc cgtcgtcata aatgccaaac tccattaacg gtttggcttt
     9961 gtcattttgt attggtatgt tttcgtacaa ttttattgca tcataattta tgattccaac
    10021 tgcgccgcca aggtacctaa acgatgtgtc gtttgtttgc ctgacaaagt ttttgagctc
    10081 agctactgga tcctgtgtcc gtatggtctt tgtggtgcca tccttgtatt gcagaacaac
    10141 tctgtcttca taaccttgca aaattatttt tggatcaaac ccaataattg atgtttcagc
    10201 aagctcttcg gggcctgtca atgattcaaa cagaaacgtg tgagaatagt tttgttctag
    10261 cttggagtaa atctcaaact gtgatccagg tagatctagg ggtattactg taggattgga
    10321 atttccaaag atggccaccc atacgacaca tcatagacgt ggcttattta taattttgag
    10381 acttttctgc ttccattctt tatttctcag ttacggtatt gtacggtacc tttataagcg
    10441 atcaagc
//
LOCUS       DASWKH010000139         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2947067, whole genome shotgun sequence.
ACCESSION   DASWKH010000139 DASWKH010000000
VERSION     DASWKH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2947067"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGA92_09860"
     CDS             complement(<1..233)
                     /locus_tag="VGA92_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016939923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9846745.1"
                     /translation="MQIGVTGPSPSDVAVKTIKTILDNYGLSSVYIGSKPRKDIDFVI
                     VTGGDKGVRNYYHRTQEPQTPILGISESESSGF"
     gene            complement(266..1138)
                     /locus_tag="VGA92_09865"
     CDS             complement(266..1138)
                     /locus_tag="VGA92_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007401722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX9846746.1"
                     /translation="MKIGIFSHCTIDEIKIGGNLYERPGGPACYGSFAARRLGFDVEL
                     VTKFGSDFTFLDQLQENKIKFQNTPSPNPTTRFTLEINESERTLWLKNICDEIEYQSV
                     NADGVLVSPVFNEIAKPTFEKIKKDNNMVFLDPQGFLRRVDSNNKVFFEKMDLDLTKI
                     SVLKADPNEVFHLTGVQGVDGVHMLRKKGIEHILYTNKRDVSLFYKNRQYSLVLPNVD
                     VYDTVGVGDIFTATFCCTLLKERDVLWALSFAGGAAQAALESKEVGLSKTPSKGATQT
                     NASYFYNTLKFKDI"
     gene            1347..>2910
                     /locus_tag="VGA92_09870"
     CDS             1347..>2910
                     /locus_tag="VGA92_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9846747.1"
                     /translation="MRNPIPVTLDKKLVTLVVLASLIGIGITIALSFHYGNIIIEERI
                     IDQLTSESTIRGDSLTSLLTAKIQQVQVISTDPMIRNLINEFNAIEDDSVFDAGVAER
                     RIDFLIQIQAIEISIGGAFDLENVEIVNKDGKRLFALINTPDEKNFESDPVFRKGLGI
                     SFAEFTLGKDDKRKLVIATPIFSSPKDVSAIGVAIVTANTQSVDQILLNRQGLGETGE
                     SYLVNSDRVLVSESRFIENAAFKQKIDTFPVELCFNSNQNHVGRYTDYRGTVVFGASY
                     CMIGSGLTLFVELDNEEVFEPVSTLRERMVMLGVVITITVGIVTYFLSKSLSIPLIML
                     QRAANRIAGGDFNVRTNIRTEDEIGQLSRSFDQMAEKIQDSLLKIKEREEIIKQQKDI
                     LLQFSEYSSNYCVCFVDIVGSTKLTSRLTDLQTSKFYSIFLNSLATVITKNGGVVVKN
                     IGDALLYYFPKTDVEDIAPFEDMLKCCMKVLDAREMINISLREESLPEISYKISATYG
                     PVRVAIIATSAID"
BASE COUNT          860 a          590 c          585 g          875 t
ORIGIN      
        1 aaaaagccac tggattctga ctcgcttatt ccaagaattg gcgtttgtgg ttcctgggtt
       61 ctgtgataat agtttcgaac tcccttatca ccgccggtca ctattacaaa atcaatgtct
      121 tttcgtggtt tgcttccaat gtaaactgat gaaagtccgt aattatcgag tattgttttg
      181 atggttttta ccgcaacgtc tgacggacta ggaccagtta ctccaatttg cacgaatcat
      241 taacctctag cataataata aaagcttaga tatctttgaa ttttaaagtg ttataaaaat
      301 aggaggcgtt agtttgtgtt gcgccttttg agggagtctt actcaatcca acttcttttg
      361 actctagtgc ggcttgcgca gcaccccctg caaagcttaa cgcccataaa acgtctcgct
      421 ctttgagcag tgtgcagcaa aacgttgcag taaagatgtc acccacacca acagtatcat
      481 agacatccac gtttggaagt acaagtgagt actgcctgtt tttgtaaaac aaagagacgt
      541 cacgcttgtt tgtataaagt atgtgctcta ttcctttttt gcgaagcata tgtaccccat
      601 caactccttg cacgccagtc aagtggaaaa cttcgttagg atctgccttg agtactgaaa
      661 tctttgtcaa gtccagatcc attttctcaa aaaaaacctt attgttggaa tcaacacgtc
      721 gcaaaaaacc ctgtggatca agaaaaacca tgttgttgtc ttttttgatt ttctcaaatg
      781 tcggttttgc tatctcgtta aaaactgggc ttaccaagac accgtctgca ttaactgatt
      841 ggtattctat ttcatcacaa atgtttttca accatagggt tctttcagat tcgtttattt
      901 ccagggtaaa ccgcgttgtt gggtttgggc taggtgtgtt ttgaaatttg attttgtttt
      961 cttgcaattg atcaaggaat gtaaagtctg atccaaactt tgtaaccagt tccacatcaa
     1021 agccaagcct tcttgcggca aaagagccat aacatgcagg tccgccaggt cgctcgtaca
     1081 gatttccacc tatcttaatt tcgtctattg tacagtgtga aaatatgcca atcttcattt
     1141 tatttgttca cggagttgtg gatttagttt tttgcctcga aaaacaagac tggcacataa
     1201 tcttcccttt ttctatgatt agctccacat ttgatctcag gcatacgtgg catagccctc
     1261 tcatcacatg ttataccacc cactagaata aatccgaata tggtaatctt agaaaaggag
     1321 atatcctaat ttccttcgaa aaaatcatga gaaatccaat tcctgtcact ctggataaaa
     1381 agcttgtaac ccttgtagtg ctggcctctt taattgggat tggcattacg attgcacttt
     1441 cgtttcatta tggcaacatc ataattgaag aacgcatcat agaccagctt acaagcgagt
     1501 caaccatcag aggcgattcg ctcactagtt tgctgactgc taaaattcag caggttcaag
     1561 ttatttctac agatccgatg attagaaatc tcatcaacga gttcaacgcg attgaggatg
     1621 actctgtttt tgacgcaggg gttgctgaaa ggcgaatcga ctttctaatt caaatccagg
     1681 caatcgagat aagtattggc ggcgcatttg atcttgagaa tgtcgaaata gttaacaaag
     1741 atgggaaaag gctttttgct ctgataaaca cgcctgacga aaaaaacttc gagtctgatc
     1801 ctgtctttcg caaggggctc ggcatatcct ttgcagagtt cacactagga aaagatgata
     1861 aaagaaaact agtcatagca actccaatat ttagcagtcc aaaagatgta agcgcaatcg
     1921 gtgtagctat agttacggca aacactcagt cagttgatca gattttgcta aatagacagg
     1981 ggctgggcga aactggtgaa tcatatcttg taaactccga cagggttctg gtttcagagt
     2041 cccggttcat tgaaaacgca gccttcaaac aaaaaattga cacgtttcca gttgagttgt
     2101 gttttaatag taatcaaaac catgtggggc ggtacaccga ctatcgggga actgtagtat
     2161 ttggcgcatc atactgcatg ataggatctg gtctgacttt gtttgtggaa cttgataatg
     2221 aagaagtttt tgaaccagtt tcaacattgc gtgagcgaat ggtcatgcta ggagtagtaa
     2281 taacaataac ggtagggatt gtaacgtatt ttctctctaa aagcctctca atacctctaa
     2341 tcatgcttca acgtgctgca aacagaatcg caggaggaga tttcaacgtt cgaacaaaca
     2401 ttagaaccga agatgaaatt ggacagttgt ctcgctcctt tgatcaaatg gcggaaaaaa
     2461 tccaggattc gcttctaaaa attaaagagc gtgaggagat tatcaaacag caaaaagaca
     2521 tactgctgca attttcagag tacagctcga attattgtgt ctgttttgta gacattgtcg
     2581 gctcaaccaa actaacctca aggcttactg acctccagac aagcaaattt tattcaatat
     2641 ttctgaactc gcttgcaaca gtcatcacta agaatggtgg cgtcgttgta aaaaacatag
     2701 gcgacgcgtt attgtactat ttcccaaaga ctgatgttga agacatagct ccatttgaag
     2761 acatgctcaa gtgttgcatg aaagtacttg atgcaagaga aatgatcaat atctcattgc
     2821 gtgaagaatc cttgcctgaa atcagctata aaataagtgc aacatacggt cctgttcgtg
     2881 tcgcaattat cgcaacatct gcaattgatg
//
LOCUS       DASWKH010000140         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_2953071, whole genome shotgun sequence.
ACCESSION   DASWKH010000140 DASWKH010000000
VERSION     DASWKH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953071"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="aroA"
                     /locus_tag="VGA92_09875"
     CDS             <1..1023
                     /gene="aroA"
                     /locus_tag="VGA92_09875"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007550017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9846748.1"
                     /translation="IVDASNSGTSIRIAAAVAALREGKTVLTGDESLKKRPMKPLLDA
                     LESLGAKCSSNDGRPPLTISGRMRGGEVSIPGNVSSQFISALLVAAPLTEKGVALNII
                     SDLVSKPYLDATIITMEKFDVKVKVLDQYKKYKIEPQQYNSTTITIPSDFSSVALLLS
                     AAVLVGRDLTIKASVGNLAQGDEAIIDILGKLGVNVSMDSNTITVKSPEKLRGGKFDL
                     ANTPDLLPPLSILALKSEFPIELYNVRHARFKETDRIAILARELQKLGINVTEKEDGL
                     ILGPPKELRGASLNSENDHRLFMAFCIAAMYVGGCTVSDSDSVDVSYPSFVSDMSRAG
                     AKISTH"
     gene            1057..2151
                     /gene="aroC"
                     /locus_tag="VGA92_09880"
     CDS             1057..2151
                     /gene="aroC"
                     /locus_tag="VGA92_09880"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9846749.1"
                     /translation="MAGSSIGQRLVLTSFGESHGKCVGAVLDGCPAGLELQEKDIQKM
                     LDQRRPGQSLVSTQRKETDRVEILSGVFRGYTTGAPIAMIIWNKDQDSKAYDQLATKM
                     RPGHSDYPALVKYKRFNDYRGGGRFSGRLTATHVMGGAIARKLLKVSLGVETNSYTLK
                     IGKITMREKFQLKMKNRIYENEVRCPDQKTAQKMRQSILSARKSGDSLGGVIESTTVN
                     VPVGLGEPIFSSLESDISKAIYSIPSVKGVEFGSGFAGSELYGSQNNDPYIVKGGKVL
                     TRTNNAGGILGGLSNGMPIVMRIAFKPASSISKKQNTIDIKTKKPVTLQVQGRHDPCV
                     VPRAPPVVDSLVSLVLADHALIGGFIKPVL"
     gene            2154..3524
                     /locus_tag="VGA92_09885"
     CDS             2154..3524
                     /locus_tag="VGA92_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9846750.1"
                     /translation="MSDIDQLRNKINDVTLQMIRLLKERTDIARQIGDLKKSSGLGVF
                     DETREDQLRNEIVTLCQEIGLDESLGKKFLNFLLNESIKVQTDGKQTHLTIFLKAKNL
                     EQQGKKIIHMEVGEPDLAPPKIVKKALGEAVDRGHVRYGQAAGMPQLRTALAGYVSEK
                     FGTQVTQENILVSPGARFSIYLAVSTLLDPGDEIIIIEPAWPAYRDCAINAGIKVRSI
                     ATTLENKWEPSIEQIQKTINANTKMIVLNYPNNPTGKVLAPQIQDKIIDVAQKNDLYV
                     LSDEIYSAYAFSEWKSVLSNNYKKSIITQSFSKSHAMTGFRIGYTVAEPKIIEKMTKL
                     QALCLTNVAEPIQYAALKALQAKVSDNVKSVRSKLDTLTGQAKKMDLEFVAPDGAMYL
                     FAKINKPGFDGVEFANKLLDYGVAVAPGDGFGNYKSFVRISACQDEKKLIVGMKILDE
                     VLRGSR"
     gene            3521..4375
                     /locus_tag="VGA92_09890"
     CDS             3521..4375
                     /locus_tag="VGA92_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX9846751.1"
                     /translation="MKKSITIIGAGGKMGLWFAKYFTSKDYEVIGYDSETPITDKTIK
                     KSESLVGSVLQADIVLLCTPTRRTPEIIRLIAKEMKRGSYLIEITSQKAKTASALLKI
                     PAKINPVCIHPMFGPGTKNIKGQNIIAVPIKDAKKELSLVKSLFEGANFVTIDAAEHD
                     KKIAIILGLTHLVNLAFANILAKDDKISLTERMSGTTFKVQKILAESIMTESTELIET
                     IISNPEIRRAAEEFWKDIGRLLTDIQEAKSEDITNYIRNIQERLAKNSNLEESYKKLN
                     VMARAIEK"
     gene            4375..>4756
                     /locus_tag="VGA92_09895"
     CDS             4375..>4756
                     /locus_tag="VGA92_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9846752.1"
                     /translation="MRSTKIVTSFVTNSQKFLILKRSNRVKSMKGLWGAISGIIEAGE
                     EPLERAKIEIFEEIGANNNAVRLLKAAKEMTISSTQYPDHQWIVYPFLFALSSAKITL
                     NWENESYKWIAPDEIYHYKTVPSLD"
BASE COUNT         1530 a         1075 c         1100 g         1051 t
ORIGIN      
        1 atagtcgacg catcaaattc tggaacatct atccgaattg cagctgcagt tgcggcctta
       61 agggaaggca aaactgttct gacaggagac gagagcctca aaaaaagacc catgaagccg
      121 ttgctggatg ctctggaatc acttggtgcc aaatgttctt caaatgacgg caggccacca
      181 ctcaccatat caggcaggat gagaggtggg gaggtatcaa tccctggaaa tgtttcaagc
      241 cagttcatct ctgcactttt ggttgccgca ccattaacag aaaaaggagt ggcacttaac
      301 atcatctcag atctcgtatc aaaaccatac cttgacgcca caatcatcac aatggaaaag
      361 ttcgatgtca aagtcaaagt actagaccaa tacaaaaaat acaaaatcga gccacagcaa
      421 tacaattcca ccacgataac aatcccctca gacttttcaa gtgtcgcatt actcctctct
      481 gccgcggtgc tagttggccg cgatctcaca atcaaggctt cagttggaaa tttagcacag
      541 ggtgatgagg caataataga catacttgga aagcttgggg tcaacgtctc catggacagc
      601 aacaccataa cagtaaaatc acctgaaaag ttgcgaggag gaaagttcga ccttgcaaac
      661 acacccgacc ttttgccacc actttcaatt cttgccctaa aatcagaatt tccaatagaa
      721 ttgtacaatg tcagacatgc gcgctttaag gaaactgaca gaatcgcaat tcttgcgcgc
      781 gagctccaaa aattaggcat caatgtgaca gaaaaagagg acggcttgat tttgggtccg
      841 ccaaaagaac tcaggggagc ttcgctaaat tcggaaaacg accacagact attcatggcg
      901 ttttgcattg ctgcaatgta tgtgggcgga tgcactgtct cagactcgga ttcagtcgac
      961 gtttcttatc caagctttgt ttccgatatg agcagggccg gagcaaagat ctccacccat
     1021 taagggttat atgcatcaaa ttggggtttt aatttgatgg ctggcagctc tattggtcaa
     1081 agactagtgt tgacaagttt tggtgagagc cacggcaagt gtgtcggagc tgtactcgat
     1141 ggatgtccag caggactgga gctgcaagaa aaagacattc agaaaatgct tgatcaaaga
     1201 aggcccggcc agtcactagt atcaacacaa cgcaaagaga cagaccgagt cgagattctt
     1261 tctggcgttt ttaggggata caccaccggc gcacccattg caatgataat ttggaacaag
     1321 gaccaggact ccaaggccta cgaccagctg gccacaaaga tgaggcccgg ccactcagac
     1381 tatcccgctt tagtgaaata caaaagattc aatgattata ggggcggcgg gcgtttttct
     1441 ggcaggctta ctgcaacaca cgtcatgggc ggcgccattg caagaaagct gctcaaggtt
     1501 tctttgggag ttgagacaaa ctcgtacaca ctcaagattg gcaaaattac aatgcgagaa
     1561 aaatttcagc taaaaatgaa aaaccggatt tacgagaacg aggtaagatg ccctgatcag
     1621 aaaacggcac aaaagatgcg ccaatcaatt ctgtctgcaa gaaaaagcgg cgactcgttg
     1681 ggcggagtaa tcgagtccac cacagtcaac gtcccagtag gactaggcga gcccatattt
     1741 agttcgcttg aatcagacat tagcaaggca atctattcca ttccttccgt aaagggcgtc
     1801 gagtttggct cagggtttgc gggctccgag ctgtatggct cacagaacaa cgacccctac
     1861 atcgtaaagg gcggcaaagt tcttactagg actaataatg ccggcggaat actcggcggg
     1921 ctctcaaatg ggatgccaat agtaatgagg attgctttca agccggcatc atcaatatca
     1981 aaaaaacaaa acacaatcga catcaaaaca aaaaaaccag taacactcca agttcaggga
     2041 aggcacgacc cttgcgtagt tcctagggcc ccacctgtag tcgactcact cgtatcgctc
     2101 gtacttgcag atcatgcgct aattggtggc ttcataaaac cagtattgta aaaatgtcag
     2161 acattgatca gctgcgaaac aaaataaacg acgtcacgct ccagatgata agactcctca
     2221 aggagcgaac cgacatcgca agacagattg gtgatctcaa aaagagcagc ggccttggcg
     2281 tttttgatga gacccgtgag gaccagctgc gaaacgagat agtcacacta tgtcaggaaa
     2341 taggactaga cgaatcgctt ggaaaaaagt ttctcaactt tttgttaaac gagtcaataa
     2401 aggtccagac cgacggcaag caaacacacc tgacaatatt tctaaaggca aagaacctcg
     2461 agcagcaggg aaaaaaaatc atccacatgg aggtaggtga gccagacttg gcgcctccaa
     2521 aaatagtaaa aaaagcgcta ggcgaggcag tcgacagggg ccatgtaaga tacgggcagg
     2581 cagccggcat gccccagctt aggactgcgc tggcaggata tgtatcggaa aagtttggca
     2641 cacaggtaac gcaggaaaac attcttgttt cgcccggtgc aagattctca atttatcttg
     2701 cagtctcgac tcttttggat cctggcgatg agatcataat aatagagcct gcatggccgg
     2761 cgtatcgcga ttgtgcaata aatgccggaa taaaggtaag gtccattgca acaaccctag
     2821 aaaacaagtg ggagccctca attgagcaaa tccaaaaaac aatcaacgca aacacaaaga
     2881 tgatagttct gaactatcca aacaacccga ctggcaaagt tcttgcgcca cagatccaag
     2941 acaagataat cgatgttgcg caaaaaaatg atctttacgt cctaagcgac gagatttact
     3001 ctgcgtatgc atttagcgag tggaaaagcg ttctctcaaa taactacaaa aaaagcatca
     3061 tcacgcaatc gttttctaaa tcgcacgcaa tgactgggtt tagaatcggg tataccgttg
     3121 cagagccaaa aatcattgaa aagatgacaa agctccaggc cctatgcctt acaaatgtgg
     3181 cagagccaat ccagtacgcc gcactcaaag ccctgcaggc caaagtatca gataatgtaa
     3241 agagcgttcg ctcaaagctc gacactctga ctgggcaggc aaaaaaaatg gacttggagt
     3301 ttgttgcccc agatggcgcg atgtatcttt ttgccaagat aaacaagccc ggctttgatg
     3361 gtgtcgagtt tgcaaacaaa ctgctcgatt acggagttgc agttgcccca ggcgacggat
     3421 ttggcaacta taagagcttt gttagaattt ctgcctgtca ggacgaaaag aaactaattg
     3481 taggaatgaa gatattagat gaggttctaa ggggatccag atgaaaaaaa gtattacaat
     3541 aattggggct ggcggaaaga tgggtttgtg gtttgccaag tattttacat caaaagacta
     3601 cgaggtaatt ggctacgatt ctgagacccc aattactgat aaaactatca aaaagtcaga
     3661 atcgcttgtc ggttctgttc ttcaggcaga cattgtactg ctttgcacgc cgacaagacg
     3721 cacgcccgaa atcataagac tcattgcaaa ggagatgaaa cgcggctcat acctaattga
     3781 gatcacctcg caaaaggcaa agactgcctc tgcacttttg aaaattccag ctaagatcaa
     3841 ccccgtctgc attcacccga tgtttggccc cggaacaaaa aatatcaagg gccaaaacat
     3901 aattgctgtt ccaatcaagg atgcaaaaaa agagctgagt ctagtcaagt ccctttttga
     3961 gggggccaac tttgtcacaa ttgacgcagc agaacacgac aaaaagattg caataatttt
     4021 aggattaact catttggtga acttggcgtt tgcaaacatt ttggcaaagg atgacaagat
     4081 ttccctaact gagcgcatgt ccggcacaac cttcaaggtc caaaagattc ttgccgaaag
     4141 cataatgaca gaatcaactg agctcattga gacaatcatc tcaaatccgg agataagaag
     4201 ggctgcagaa gagttttgga aagacattgg gcggctgctc accgacattc aggaggccaa
     4261 atcagaagac atcaccaact acatcagaaa catccaagaa aggcttgcga aaaattccaa
     4321 ccttgaagaa tcctacaaga agctaaacgt gatggcacgc gcaatcgaaa aataatgcgt
     4381 tcaacaaaaa tagtaacctc ttttgtgaca aacagccaaa aattcctcat actcaaacgg
     4441 agcaacaggg taaaaagcat gaagggtttg tggggcgcaa ttagtggcat aatcgaggca
     4501 ggggaggagc cacttgagcg ggcaaaaatt gagatcttcg aagaaattgg tgcaaacaat
     4561 aacgccgtga ggctgctcaa agcggcaaag gagatgacga tttcttcgac ccagtatccc
     4621 gaccatcaat ggatagttta cccatttttg tttgcgctaa gctcggcaaa aattacgctg
     4681 aactgggaaa acgagtcata caagtggatt gcgccagatg agatttacca ctacaagaca
     4741 gtcccaagcc ttgatg
//
LOCUS       DASWKH010000141         4876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3655479, whole genome shotgun sequence.
ACCESSION   DASWKH010000141 DASWKH010000000
VERSION     DASWKH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655479"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGA92_09900"
     CDS             complement(<1..291)
                     /locus_tag="VGA92_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013481332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9846753.1"
                     /translation="MALDFLAQNSIIYVLVAWVAILGVAKALKLDKHGFAIKPYSLTY
                     KNTQVQVALTKMLGRTRRGIRVFADVSVIAGFIMMGFAFWFLLDNVVKYFDRP"
     gene            complement(434..595)
                     /locus_tag="VGA92_09905"
     CDS             complement(434..595)
                     /locus_tag="VGA92_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846754.1"
                     /translation="MTNRSAISHNENTKGNADRILSVVMGQNFPDRELAENIRQASEE
                     RRKHFKIRK"
     gene            complement(595..828)
                     /locus_tag="VGA92_09910"
     CDS             complement(595..828)
                     /locus_tag="VGA92_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HEX9846755.1"
                     /translation="MCTTTISLSNEAYKILKAQKMEGESFSDLILRKFGKGNPSAILA
                     YLKKEGPNPDLADAVEKASREFRKNFRTRKVSL"
     gene            907..1218
                     /gene="cutA"
                     /locus_tag="VGA92_09915"
     CDS             907..1218
                     /gene="cutA"
                     /locus_tag="VGA92_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX9846756.1"
                     /translation="MKPAIIVSTYPDKKSISKIANKLAKDKLAACVNITKISSIYAWQ
                     GKIENTDEFLAVFKTTQKNKSRLKKEIKKTHPYKVPEIAELGINDINKPYLQWLIDST
                     G"
     gene            complement(1215..2267)
                     /locus_tag="VGA92_09920"
     CDS             complement(1215..2267)
                     /locus_tag="VGA92_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein Pelota"
                     /protein_id="HEX9846757.1"
                     /translation="MITKIIDDSSISFLVEEPDDLLTLRRVIKKEDKIISDTTRVIKQ
                     EKDYARPDRGERIRIRISLEVEKILLDSVFDKLRIHGTIIESTSEEVSKGVHHSILIR
                     IGDSLNVVKKKWPPFELRLIKKKDDDFGFALIAIDKGDCGIGKLKGTHLQLMQNLYSG
                     SSGKQYKTDFNIKAFFQTVTKALETVLRENDLIIIFGPGETKRQFSNFLQKTPLAKNH
                     KVEIVDGIDSGGEDGIYTFIKSKSMHQILGQSKLAKVATILEDVMIKAQHKSKKFTMG
                     FEETKKANQFGAVESLVFSDKIIQTHDEEQIIEFLNEVEAKGVKVYALDSSTDIGLQV
                     SGLGGMVSLLRFPVDS"
     gene            complement(2294..2704)
                     /locus_tag="VGA92_09925"
     CDS             complement(2294..2704)
                     /locus_tag="VGA92_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846758.1"
                     /translation="MVAIDVPASLESFRRFVIASTCSSYIPRSYIEDQEVFPEREETL
                     GSIYVEAADKVTLKKIRDITFVNARDVLGIIYNSKSGNTTLKWRQLRRKGGKVTGEAS
                     PNSLVNLSEGGVITPEWVDNYLKKKSLENTTSTV"
     gene            2904..3323
                     /locus_tag="VGA92_09930"
     CDS             2904..3323
                     /locus_tag="VGA92_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX9846759.1"
                     /translation="MKILTEHLTFNTKTKVAFVNLTPQIRQLVKKSGIKEGLCLVNAM
                     HITASVFINDNESGLHKDYARWLEKFAPHEPIEQYDHNKTGEDNADAHLKRQIMGREV
                     VVAITDGELDFGPWEQIFYGEFDGMRSKRVLVKIIGE"
     gene            complement(3330..3566)
                     /locus_tag="VGA92_09935"
     CDS             complement(3330..3566)
                     /locus_tag="VGA92_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX9846760.1"
                     /translation="MSEISVGSITTKGRITIPKEIRERLELKEGDKVIFVIKTEQAII
                     RKASEEKLSEILRRQKPWREHSVKFQKRIRKKWQ"
     gene            3946..4215
                     /locus_tag="VGA92_09940"
     CDS             3946..4215
                     /locus_tag="VGA92_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9846761.1"
                     /translation="MSTQYKQLFTVKVIATDEGFIGKCLELPGAISEGNTLEELKKNM
                     TEAIKLILEEINESSKNDYKMIIEVIREDKFPITQEDELMERVKA"
     gene            complement(4312..4758)
                     /locus_tag="VGA92_09945"
     CDS             complement(4312..4758)
                     /locus_tag="VGA92_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX9846762.1"
                     /translation="MDSANDEVLFVGTAEAEHVEMYLKAIWHIKERNEPVKISTIAKM
                     LNVRQPSVVQMLKKLNEQNLVNYSKAGVSLTESGEIVGSKMMRNSRLLEVLMDSALKV
                     EVDEEMVCGIEHHMSQEFTDALCTMLKHPRKCPHGHAIPRGVCCTN"
     gene            complement(4758..>4876)
                     /locus_tag="VGA92_09950"
     CDS             complement(4758..>4876)
                     /locus_tag="VGA92_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013482271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HEX9846763.1"
                     /translation="LSESPEGEAVAIWADHAGLAGFAREYFEYLLKDAKGVE"
BASE COUNT         1375 a         1050 c          908 g         1543 t
ORIGIN      
        1 aggcctatca aaatatttta ccacattatc aagcaaaaac caaaaggcaa atcccatcat
       61 aataaaacct gcaataacgc tgacatcggc aaatacgcgt atgccgcgtc gcgtgcgccc
      121 aagcatcttt gttagagcca cttggacttg ggtatttttg taggtgaggc tgtacggttt
      181 tatggcaaag ccgtgcttgt ctagctttag tgctttagct actcctagta ttgccaccca
      241 cgcaactagg acataaatta tagaattttg cgccaaaaaa tctaaagcca aattaattat
      301 gttctctatt tgtggtcatt tataaaaatg aagcgaggca tttgacataa tttcttaatt
      361 aatccttctt ttctattact gtgatcaatt aaaaaatctg tatcgattta caccatgatc
      421 tacctattac ctattatttt ctaattttaa agtgcttacg gcgttcctca cttgcctgcc
      481 ttatgttttc agcaagctct ctgtcaggaa agttttggcc cattacaaca gacaggattc
      541 tatcagcgtt gccttttgta ttttcgttgt gtgatatagc tgaacgattc gtcatcagag
      601 actgaccttt cttgttctaa aattcttgcg gaattcgcga ctggcctttt ctactgcatc
      661 tgccaagtcc gggtttggac cctctttctt tagataggct aggatggcag acggattacc
      721 ctttccaaac ttacgcagta tcaaatccga aaaagactct ccttccatct tctgagcttt
      781 tagtatcttg tatgcctcat tgctcaagga aattgttgtt gtacacatat gcaatataat
      841 ataggtacac acagttaaag cattccaaac ccaaaagaac aaacatcagg aagtttaccg
      901 aattctatga agcctgcaat aattgtttct acatatccag acaaaaaatc aatttcaaaa
      961 attgccaaca agcttgcaaa agacaaactt gctgcgtgcg tgaacataac aaaaatctct
     1021 tcgatttatg cttggcaggg aaaaatcgaa aatactgatg agttcctggc agtctttaag
     1081 acaacgcaaa aaaacaaatc aagactgaaa aaagagatta aaaaaacaca cccctacaag
     1141 gttccagaaa ttgcagaact tggaataaat gatatcaaca agccatatct gcagtggcta
     1201 attgactcta caggctagga atccactggg aaccttagca aggagaccat tccaccaagt
     1261 cctgaaacct gcaatcctat gtcggtgctg gaatccaacg cgtatacttt gacacctttg
     1321 gcttctactt cgtttaagaa ttcgattatc tgttcttcat catgggtctg gatgatcttg
     1381 tctgagaaca ccaaggactc tactgcacca aactggtttg ccttttttgt ctcctcaaag
     1441 cccatcgtga attttttgct cttgtgctgc gctttgatca tcacatcttc taagatggtt
     1501 gcaacctttg caagcttgct ttgccctagt atctggtgca ttgactttga cttgataaac
     1561 gtgtaaattc cgtcctctcc gccagagtca attccatcta ctatctcgac tttgtggttt
     1621 tttgccaggg gggttttctg caaaaaattg ctaaactgcc tttttgtctc gcctgggcca
     1681 aaaattatta tcaaatcgtt ttccctgagg actgtctcta gcgcttttgt gacagtttga
     1741 aaaaaagcct tgatgttaaa gtctgtcttg tattgcttgc cactagagcc ggagtaaaga
     1801 ttctgcatca gctggaggtg cgttcctttg agcttgccga ttccgcaatc gcctttgtct
     1861 attgcaatta gggcaaaacc aaaatcatcg tctttctttt ttatgagtct tagctcaaag
     1921 gggggccatt tttttttaac aacgttgagc gagtcgccaa ttctgatcaa aatggaatgg
     1981 tggacgccct ttgatacctc ctcactagtc gactctatta tggttccgtg gattctcagc
     2041 ttgtcaaata cggaatctag caatattttt tcaacctcaa gcgatatcct tattctgatt
     2101 cgctctcccc tgtctggcct ggcataatcc ttttcctgct tgattactcg tgtagtgtct
     2161 gatatgatct tgtcctcttt ttttatcacc ctgcgaagtg tgagtaaatc gtctggctct
     2221 tcgactagaa atgagatcga tgaatcatcg ataatttttg taatcatcaa cttgcaggta
     2281 tttgataaaa taattaaaca gtgctagtag tgttttctag gctcttcttt ttgagataat
     2341 tatcaaccca ttctggcgtg attactccgc cctctgataa attcacaagt gagtttggcg
     2401 atgcctctcc tgtaaccttg ccgccctttc ttctaagctg tctccacttt agtgtggtat
     2461 tgccgctttt ggagttgtaa atgattccta gcacatctct tgcgtttaca aaggtgatat
     2521 cgcgaatctt ctttagggtg accttgtcgg ctgcctcgac gtatatggaa ccaagcgttt
     2581 cttcgcgctc tgggaagact tcctggtctt ctatgtaact gcgtggaatg tacgaactgc
     2641 acgtgctggc aatcacgaat ctcctaaagc tctcaagtga tgctgggacg tctattgcaa
     2701 ccatttcttt tatgatacct ctttgccatt tatcaagctt cagttaaaaa tcccttttgt
     2761 ttactatgat acgtaggcgt tgatgacata atccctttga tcttgatgat gtagatcttg
     2821 agttctgagc ctgcagtaca gttacgcata ttggaatatt gataataatc gagtatttat
     2881 tttccaaaat tgatttctaa tgcgtgaaaa tccttacaga acatctgaca tttaacacta
     2941 aaaccaaagt tgcttttgta aacctgacgc cacagatacg gcagctagtc aaaaaaagcg
     3001 gaatcaaaga aggcctttgt cttgttaacg caatgcacat tactgcaagc gttttcataa
     3061 atgacaatga aagcggattg cacaaagatt atgcaagatg gcttgaaaag tttgccccac
     3121 acgaaccaat agaacaatac gatcacaaca aaacagggga ggataatgca gatgcacacc
     3181 taaaaaggca aatcatggga cgtgaagtcg tagttgcaat tactgatggc gagctggact
     3241 ttggtccatg ggagcaaatc ttttacgggg agtttgatgg tatgagatca aagcgcgttc
     3301 tggtaaagat aatcggggaa taatttaggt tattgccatt ttttacgtat tcttttttgg
     3361 aatttgacac tgtgttctct ccaaggtttc tgcctacgta gaatttcaga gagcttttct
     3421 tctgatgcct ttcttattat tgcctgttct gtcttaataa caaaaattac tttgtcgcct
     3481 tcttttagtt ccaatctctc tctaatctcc tttggaatcg ttattcggcc tttggtggtg
     3541 atagaaccca cactaatctc tgacattttc ttaccatata gtaggcattc cttactaaaa
     3601 tattacaaga ttgttttgga aaaaatttca cagaaatcat gaaatctatt aagaaatact
     3661 tgaagttcaa cacaaaaacc gttttagaca aaattattga ctaatgattt acccaaaatc
     3721 tacgtcgcgt ttctgactct gtgtctcatt ttttcttgca gctagtctaa actcttcttc
     3781 gtgattttgt ggtccatgac tacacttgac acatccaact ttgaagccat ttggatcctt
     3841 gacatatgtc tggcaaccac aaaaacatac cataactaac gccaatcata gagtgatata
     3901 ttcgatacta taccttaatt tagaaaggaa gcaaaataag atctattgtc aacacagtac
     3961 aaacaattgt tcacagtaaa ggtgattgca actgatgagg gttttatagg aaaatgtctt
     4021 gagcttcctg gagccataag tgaaggcaat acgcttgaag agctaaagaa aaacatgact
     4081 gaagcaataa agctaatttt ggaagaaatc aatgaatcct ctaaaaatga ctataagatg
     4141 ataatagaag taatacgtga ggacaaattt ccaataacac aagaagacga acttatggaa
     4201 cgagttaaag catgaaaaga gccatccaaa acatttgata gtcttgaaga cctgttagat
     4261 gaactaagtt cctaattgta tataagacaa atcatagaaa atagaattat cttaatttgt
     4321 gcagcaaacc ccgcgcggaa ttgcatgccc gtgtggacat ttgcgcggat gctttagcat
     4381 tgtacataac gcatctgtaa attcctggct catgtgatgt tctattccac agaccatttc
     4441 ctcgtcgact tctaccttta gggcgctatc cattagtacc tccaaaagcc tgctgtttcg
     4501 catcattttg gagccgacga tctcgccgct ttcagtcaac gaaactccgg ccttgctgta
     4561 gtttaccagg ttctgctcgt ttagcttctt tagcatttga accacgcttg gctgcctgac
     4621 gtttagcatc ttggcaatag tactaatctt gactggctcg tttctttcct tgatgtgcca
     4681 tattgccttt aggtacatct ctacatgttc tgcttctgca gtcccaacaa aaagtacctc
     4741 gtcatttgcc gaatccatta ttccacaccc tttgcatctt tgagcaaata ttcaaagtat
     4801 tccctggcaa agccggcaag gcctgcatgg tctgcccaaa ttgcgactgc ctcgccttct
     4861 ggggactcgc ttaacg
//
LOCUS       DASWKH010000142         7727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Nitrosotenuis sp. isolate SMAG_U2917
            k141_3691237, whole genome shotgun sequence.
ACCESSION   DASWKH010000142 DASWKH010000000
VERSION     DASWKH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960562
            Sequence Read Archive: SRR10376087
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Nitrosotenuis sp. (soil metagenome)
  ORGANISM  Candidatus Nitrosotenuis sp.
            Archaea; Nitrososphaerota; Candidatus Nitrosotenuis.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 07:53:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,001
            CDSs (total)                      :: 1,954
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 3 of 5
            Pseudo Genes (internal stop)      :: 1 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Candidatus Nitrosotenuis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691237"
                     /isolate="SMAG_U2917"
                     /isolation_source="soil"
                     /db_xref="taxon:2507556"
                     /environmental_sample
                     /country="USA: Oregon"
                     /lat_lon="42.99 N 123.63 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGA92_09955"
     CDS             <1..377
                     /locus_tag="VGA92_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846764.1"
                     /translation="KFIEEQRQIANQDLLADLQSMVEQFDPFNPRNVFSSFVSGFDST
                     IKNIYWGQFNFTETKTKEGLAALEQVLNNGGTMQDARNAFYETAAISQSEINKLNDEL
                     NTKYAKNDTRSRSDGEHYRNKN"
     gene            697..1071
                     /locus_tag="VGA92_09960"
     CDS             697..1071
                     /locus_tag="VGA92_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008299002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6659 family protein"
                     /protein_id="HEX9846765.1"
                     /translation="MSKEKVNEYWIFEQKCKKILQEDEIRFAGIVNRMGRLVAGGFKE
                     GIRPLEDEEDRRKMYMELILRVSMRSEFDYSLGKVKYTASRREKAVVMSFPLDNNVLF
                     ISAEPDISIDKTANKILKLIGV"
     gene            complement(1382..2020)
                     /locus_tag="VGA92_09965"
     CDS             complement(1382..2020)
                     /locus_tag="VGA92_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEX9846766.1"
                     /translation="MTRLIDPSLKKLSSIMSEMGELSLQSFVLAIDSYVYGKNTVDQV
                     RDISHEISKKYFQVADLTFEILLKYQPVADDFRLIRSSIEISYGFSRFGRYAYDITLV
                     RDMFGDIAECDKSWLVEASSKVKTMIKDAVIYFAELDIRKAINIQQNEEFVDRLYKER
                     LPMLINSNNTKCALAEALVLRYLERIADHAIFMSDAINYIVTGKHKLAKASL"
     gene            complement(2021..2767)
                     /gene="pstB"
                     /locus_tag="VGA92_09970"
     CDS             complement(2021..2767)
                     /gene="pstB"
                     /locus_tag="VGA92_09970"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9846767.1"
                     /translation="MIAENVSVYYDGIQAVKNVTMKFKERTVSSLIGPSGCGKTTFLR
                     CLNRMHDMTKNARVEGKILLDGQDLYSKDVDPIYHRRKVGMVFQKPNPFPTMSIYDNV
                     SAGLRLNGVRDKKILDEIVEDSLRMAYLWEEVKDNLKKPAMELSGGQQQRVCIARALA
                     IQPEVLLMDEPASALDPIATQKIEETITDLAKEFTIIIVTHNMQQAVRVSNYTAFMYL
                     GDLIEFDETQKLFTQPRNELTAKYVKGQFG"
     gene            complement(2845..3771)
                     /gene="pstA"
                     /locus_tag="VGA92_09975"
     CDS             complement(2845..3771)
                     /gene="pstA"
                     /locus_tag="VGA92_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX9846768.1"
                     /translation="MMTPSSQKRAEYRALFKQNVKGRLIVDRIAMAVVFGCVVVAIIP
                     LGSILVEVFKNGISALSVEFLTQPPGAIGSGEGGIGPAIQGTLIMIGMSSLIGVPIGL
                     LSGIYLAEFGDNRLARTVRFFNDVFMEFPSIVLGIFAFLTIVLLLGHFSLWAGAFALS
                     LIMFPIVARTTEESLKLVPLTYREAGLALGLKKWVITLRIVFSAAKSGMITGILLAIA
                     RIGGETAPLIMTVLGSSQFFSGFDAPMDALPLRIWRLSLLPYDSAQAQGWGAAAVLIM
                     IILAINIGVRYYFATKNKKGGFIAKVLGGYKR"
     gene            complement(3768..4736)
                     /gene="pstC"
                     /locus_tag="VGA92_09980"
     CDS             complement(3768..4736)
                     /gene="pstC"
                     /locus_tag="VGA92_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9846769.1"
                     /translation="MLPHQKHNQSRFPADKIFKIAATAGGVYVLLVIGLMVFELYSES
                     HEIWEYEGLDFLFGSQWNAVEGREAFGAAPYILGTLVTAGLAMAMGVPISIGIAMFIS
                     QAPKFIANPLSVIVELLAAVPSIIYGFWGLLVFRGFLLDWIEVPLHETFGENIWLFSS
                     APFGLDIFSASIILAIMIIPTISAVSREIMIAVPHMQKEAAYMLGATKWEMFKIAVFP
                     YAKTGLIGAAILGLGRAVGETMAVTMLIGNATGPSAFPQTLFQPGQTMSSIIANEFIE
                     ASPASIHLPALIGVGLILLLVAICINVVAHLLVSRMLKVKEGVVNA"
     gene            4864..5901
                     /locus_tag="VGA92_09985"
     CDS             4864..5901
                     /locus_tag="VGA92_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007549886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEX9846770.1"
                     /translation="MQSLEDTKQTRRIQISGGSTYTISLPKKWIDELGIKNGDNMTIV
                     KNANRSMTLFPGLDTEKPSKKAVITISQKDSEESIRRKIIALYLTGYKTIQVTSKGVK
                     LLSEHSRLIKDLVRKSMIGTEIVESDSESITIQILTRLPELTFEVALKRMYLMTSNMH
                     REAIDALKKFDVEYGEEVVRMDDEVDRFSLYMMRTLIMAIQNASMLYDVGLEQPSDCL
                     NYRIVISRIERISDHAALIAKRIKFLKEPLEPKILKEIEALSSEALNCFESSILALTK
                     KDHLLAEKVAANIASVVEKEKEFMYGLKESKNSTAIKFILEDIRRTAEYSSDIVEVVI
                     NETIHNIISEK"
     gene            complement(5933..6547)
                     /locus_tag="VGA92_09990"
     CDS             complement(5933..6547)
                     /locus_tag="VGA92_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846771.1"
                     /translation="MIPQKVTNTSVIAAIAIATIALVIGLSQTQTTTAADTPVGNNVY
                     VFAEGVYPQATFKFKDATVTYEFQAFTQTSNLIGSNGRSSTPEFTLQRIAGETPYLHR
                     SVDEAFQNAGRIGIMQLPYQEFDVEIELIQAGKAIRAFEYADCQISNYKTATEYDKEE
                     GYTTGGKTGFAVMETFTFGCAGFEPIATTFNEMIEARGGNTQYD"
     gene            complement(6813..7295)
                     /locus_tag="VGA92_09995"
     CDS             complement(6813..7295)
                     /locus_tag="VGA92_09995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9846772.1"
                     /translation="MSALNDAYWDRYAADFASHHNEEMAKFIRDLALSLRVSSVLEVG
                     CSAGNDLSLFPKDLDVNGIDKSEFAIKIARQNLPFKFKVGLATSLPYDDSSIDLVFTR
                     NVLNYVESSGADQVLIELFRVSKKYILNVELFSDEESQITDEPIASCGRNMYKRWSNF
                     "
     gene            complement(7283..7447)
                     /locus_tag="VGA92_10000"
     CDS             complement(7283..7447)
                     /locus_tag="VGA92_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9846773.1"
                     /translation="MESCSVGGKGEEICWFCKKGRHSECMKEIPTEGMSDGPHDCTFD
                     TKMIPCKCQH"
     gene            complement(7485..>7727)
                     /locus_tag="VGA92_10005"
     CDS             complement(7485..>7727)
                     /locus_tag="VGA92_10005"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010192205.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HEX9846774.1"
                     /translation="QIIRADGGDIIEKDLDRDAIYSADEIFMTGTAAEVKSVTQVDDV
                     IIGDGKIGQTTQTLQKSFMDVAMGRDERFIHWLRYL"
BASE COUNT         2362 a         1612 c         1448 g         2305 t
ORIGIN      
        1 aaaaattcat agaagaacaa cgccaaatag caaaccaaga cctgctagcc gatcttcaat
       61 ccatggtgga gcagtttgac ccattcaacc cacgaaacgt attctcatcc tttgtttctg
      121 ggtttgattc aactatcaaa aacatatact ggggacagtt taattttacc gaaaccaaga
      181 caaaagaagg tcttgcggca ctagaacaag tattaaacaa cggtggcacg atgcaggatg
      241 caaggaatgc attttacgaa acggcagcaa tctcacaatc tgagataaac aagctaaacg
      301 acgaactaaa tacaaaatat gcaaaaaatg acacccgatc aaggtctgat ggggaacact
      361 accgcaacaa aaactagttt acgaattaca cacaattcac ttgtatggat aggacgggaa
      421 gctgacttgg caacatgcaa gtggtagacg attttgcctc caatttgtgc cagtcttgat
      481 acaggtcctt gggggattct gtgtcagctt ccagagtaat atcgtgtttg ttaaaaataa
      541 aggtgccttg tagcttatct gataactgtt caaatttttc atttatttta aatgaatgcc
      601 taattctcaa actgaatctt ataagcctga ttttcgtttg tttagtaatt tcctacacta
      661 ctttaagttc atttaattcc atagtactta gccatgatgt caaaagaaaa ggtaaacgaa
      721 tactggattt ttgagcaaaa atgcaaaaaa atactgcaag aagacgaaat taggtttgcc
      781 ggtatagtaa acagaatggg taggcttgtc gcaggcggct tcaaagaagg cataaggccg
      841 cttgaagatg aagaggaccg aaggaaaatg tacatggaac taatacttcg agtctcaatg
      901 agaagcgaat ttgattacag tcttggcaaa gtcaagtaca ctgcctcaag aagagaaaag
      961 gcagttgtta tgagttttcc gcttgataat aatgtcttgt ttatatcagc tgaaccagac
     1021 atatccattg acaagacagc aaacaagatt ctaaaattaa ttggcgtgtg agattttaat
     1081 tatgaaaaat gacttggtca ttatgcatat tatgttgtaa ttgaaagttt tgacaaatca
     1141 acaaatctaa aacagtttgt atgatcatca tgcgaaacaa catgacaaca ttttttgcat
     1201 atcctaagaa gaatttttct acattctatg catcgagcat aatcaattaa gcttcctcca
     1261 catttcctac aactctcatc tggcataagg atagaaatca acaaaacact aatgactttg
     1321 tccaaaatag ttttctgaca aatacatagt gtctatactg gtattgatct gcagatattc
     1381 atcaaaggct tgcttttgcg agcttgtgct ttcctgtcac tatgtagtta attgcatcac
     1441 tcataaagat agcatgatct gctattcttt caagatagcg taacactaat gcctctgcaa
     1501 gagcacattt ggtattattt gagttaatta acattggtaa gcgttccttg tataacctgt
     1561 ctacaaattc ctcattttgc tgtatgttga ttgcctttct aatgtcaagc tctgcaaagt
     1621 atatcactgc gtctttgatc atggtcttta ccttgcttga tgcttcgaca agccagcttt
     1681 tatcacactc tgcaatgtct ccaaacatgt ctctgactag agtaatatca tacgcatacc
     1741 tgccaaacct cgaaaaacca tacgaaattt ctattgatga gcgaattagc ctaaaatcgt
     1801 ctgcaaccgg ctgatatttt aataaaatct caaacgtcaa atcggctact tggaaatatt
     1861 tttttgaaat ttcatgggaa atatcacgaa cttggtctac ggtatttttt ccgtaaacgt
     1921 aagagtctat tgcaagcaca aaggactgaa gagaaagttc tcccatctcc gacattatcg
     1981 aggaaagttt cttaagagat ggatcgatta gtcgggtcat ctaaccaaat tgccccttta
     2041 cgtattttgc agttagttca tttcttggct gcgtgaaaag cttttgagtt tcatcaaact
     2101 cgatcaaatc gccaaggtac ataaatgcag tataatttga aacacggact gcctgctgca
     2161 tgttatgtgt gacaataatt atcgtgaatt cttttgcaag atcagttatt gtttcctcaa
     2221 ttttctgagt agctattggg tctagtgcgg atgccggctc atccattaaa agaacttctg
     2281 gctgaatggc aagggcacgt gcaatacaaa cacgctgctg ctggcctccg gataactcca
     2341 ttgccggctt tttgagattg tcttttacct cctcccaaag gtaagccatc ctcaaggaat
     2401 cttcaactat ctcatctagg atctttttgt ctcttacacc atttagacga agacctgctg
     2461 acacattgtc ataaattgac atggttggaa agggatttgg cttttgaaat accatgccta
     2521 ctttacgcct gtgataaatc ggatcaacat cctttgagta tagatcttgg ccatcaagta
     2581 ggatttttcc ttcaactctg gcattttttg tcatatcgtg catcctgttc aagcatcgaa
     2641 gaaaggtagt tttgccacag cctgacggcc caatcaaaga gctaacagtt cgttctttga
     2701 atttcattgt tacgtttttc acagcttgta ttccatcata atacacagag acattttctg
     2761 ctatcatctt gtaatttttt tcatcagagg aagtgttttc aatttgggag cctgtactgt
     2821 cttgttttaa atcaaaaact gtggtcatct cttatatcct cctaaaactt tggcaataaa
     2881 accgcctttc ttattctttg ttgcaaagta atatctcaca ccaatgttga ttgcaagaat
     2941 tatcattatc aaaactgctg cagcgcccca tccctgagcc tgtgcactgt cataaggcag
     3001 caatgagagc ctccatattc taagtggcaa tgcatccatt ggagcatcaa atcccgaaaa
     3061 aaactggctg cttcctaata cagtcataat cagaggggca gtttctccac caattcttgc
     3121 aatagcaagc aaaatcccag ttatcattcc actttttgct gcagaaaaaa caattcgtag
     3181 tgtgattacc cattttttta agccgagcgc aaggccagcc tctctatatg ttagtggaac
     3241 tagcttcaga gattcttctg tggttcttgc aacaattggg aacatgatga gggagagcgc
     3301 aaaggcccca gcccacaaag aaaagtgtcc gagtagtaat acgattgtca aaaatgcaaa
     3361 aattccaaga acaattgatg gaaactccat aaagacatcg ttgaagaatc gaacagttct
     3421 tgcaagcctg ttatcgccaa actctgctag gtaaatgcca gacagtaacc caattggcac
     3481 accaatcaga cttgacatgc ctatcatgat cagcgttccc tgaatggcag ggccgattcc
     3541 accctcccca cttccaattg ctccaggagg ttgtgtcaaa aattccacag aaagagcaga
     3601 gatgccgttt ttgaaaactt ctactagtat actgccaaga ggaattatag caacaacaac
     3661 acatccaaaa actactgcca ttgcaatcct gtcaacaatt agtcggcctt tgacattttg
     3721 tttgaaaagt gctctgtatt cggccctttt ttgactcgat ggtgtcatca tgcgttcaca
     3781 acaccttctt ttactttgag cattcttgaa actaggagat gtgcaaccac attaatgcaa
     3841 attgcaacaa gcagcaagat aagtcccact ccaatcagcg caggcaggtg aattgatgct
     3901 ggtgatgctt caatgaactc gtttgcaatt atgctggaca tcgtttgccc tggctggaaa
     3961 agcgtttgcg gaaatgcact tggaccagtt gcgttgccaa taagcatggt tactgccata
     4021 gtttcgccaa cagctctacc cagacctaaa attgcagctc caatcaaacc tgtctttgca
     4081 tacggaaata ctgcaatctt gaacatctcc cattttgttg caccaagcat gtatgcagct
     4141 tctttttgca tatgtggtac tgctatcatt atttcccttg aaactgccga aattgttgga
     4201 atgatcatta ttgccaaaat tatgcttgca ctaaagatat ctaatccaaa cggtgctgat
     4261 gaaaaaagcc agatattttc tccaaaggtt tcatggaggg ggacctctat ccaatcaagc
     4321 aaaaatcccc taaagacaag gaggccccaa aatccgtaaa ttatgcttgg tactgcggcg
     4381 agaagctcaa ctatgacgga gaggggattt gcgatgaatt ttggcgcctg agaaataaac
     4441 attgcaattc caatgcttat tggtacgccc attgccattg ccagccccgc tgttaccaaa
     4501 gttcccaaaa tgtatggcgc agcaccaaag gcctccctac cctctacagc attccactgg
     4561 gagccaaaca aaaaatccaa tccctcatat tcccagattt catgggattc tgaatacagc
     4621 tcaaatacca ttaatccaat aactaacaaa acatacacac cgcctgccgt tgcagcaatt
     4681 ttgaatatct tgtccgctgg gaacctactt tggttgtgtt tttggtgtgg cagcaataat
     4741 ttgatgccta ggttttggct tatctaggat ctgtacatat tctaccgaga aactgtagtt
     4801 ctatatagga caatatagta taaagaccaa aacggtatta gctatctcac ttacatctag
     4861 ttattgcagt cattagagga cacaaaacag acaagacgca tacagattag cggtggatct
     4921 acatacacaa tctcgcttcc caaaaaatgg attgacgagc ttggaatcaa aaacggcgat
     4981 aatatgacta tagtaaaaaa cgccaaccga tcaatgacac tttttcctgg acttgataca
     5041 gaaaagccat caaaaaaggc agtcattaca attagccaaa aagactctga ggaatcaatt
     5101 aggcggaaaa ttatcgccct ttatctgacc ggatacaaga caatccaggt cacatcaaag
     5161 ggagtcaaac ttctctctga gcactctcga ctgataaaag accttgtccg taaatccatg
     5221 attggaaccg aaatagtaga gtctgattca gaatcaatta ccattcaaat ccttaccaga
     5281 cttcctgagc tgacatttga ggttgcgcta aagagaatgt accttatgac ttcgaatatg
     5341 catcgcgagg caatagatgc actcaaaaaa ttcgatgttg aatacggcga agaagttgtt
     5401 cgaatggatg acgaagtcga caggttcagc ctatacatga tgaggaccct aatcatggca
     5461 atccaaaacg caagtatgct ttatgatgtg ggactcgaac aaccttcaga ctgtttgaat
     5521 tataggattg ttatctcacg tattgaaaga atatctgatc atgcggcact aatcgcaaaa
     5581 cgaataaagt ttctaaagga accacttgag ccaaaaattc taaaggaaat agaggcacta
     5641 agcagtgagg cattaaattg ttttgaaagc tcgattttgg ctttgacaaa aaaggatcat
     5701 ttgctggcag aaaaagtggc agcaaacata gctagcgtgg tagaaaaaga aaaagagttc
     5761 atgtatggac tgaaggaatc gaaaaattcc acggctatca agttcatttt ggaagacatt
     5821 cgaaggactg cggaatattc aagtgacata gttgaagtcg ttatcaacga gacaattcat
     5881 aatataatct ctgaaaaata aaaaataata aaaaagttat tgggggttga ttttaatcgt
     5941 attgtgtgtt tccgcctcta gcctctatca tctcattaaa ggtggtagct attggttcga
     6001 aacctgcaca accaaaagta aatgtctcca ttactgcaaa gcctgttttt cctccggttg
     6061 tgtatccctc ctctttgtca tactcagtag cagtcttgta gtttgatatt tggcagtcag
     6121 catattcgaa tgctcttatg gcttttcctg cctgaatcaa ttctatctca acatcaaact
     6181 cttgataagg caattgcatg atacctatcc ttcctgcatt ttgaaatgcc tcatctacag
     6241 atcggtgaag atatggcgtt tctcctgcaa ttcgttgcag tgtgaattct ggtgttgatg
     6301 accttccgtt gctaccaatt agattgctgg tttgagtaaa tgcttgaaac tcatatgtca
     6361 ctgtcgcatc tttgaacttg aatgttgctt gtggatagac gccttcggca aatacataaa
     6421 cattgttgcc tactggagta tcagcagctg ttgtagtttg tgtttgcgaa agaccaatca
     6481 ccagagcaat tgttgcaatt gcaatagccg caataacaga tgtgtttgtt actttttgtg
     6541 gaatcattag ctatcacggc atctcacgat tactaaagaa gaatccctat tgattctgta
     6601 taatacgatg gactttgtag ggtatgtggc agtacgttcc aaattgattt tttcttttta
     6661 ttttttaaaa aaactgtatg ttactaagtt ttaaaaaata ctatgtgccg tatgtaatta
     6721 tatcacagag cctatatttt tttaaccaaa gtaaaccggg ctttttttgg gtcgatgtct
     6781 tggtgcaaat ccacattact gattatcttt acttaaaagt ttgaccaacg tttgtacatg
     6841 ttgcgaccac aagacgcgat tggttcatca gttatttgcg attcctcatc agaaaaaagc
     6901 tcaacattta ggatgtattt ttttgaaact cggaacagct ctattaacac ttgatcagca
     6961 cctgagcttt caacgtagtt tagaacattt cttgtaaata caaggtcaat tgacgagtca
     7021 tcatatggaa gggatgtggc aaggcccact ttgaatttaa aaggcagatt ttgcctggca
     7081 atcttgattg caaattcgct tttatcaatc ccgtttacat caaggtcttt tggaaataaa
     7141 ctcaagtcat ttcctgcact acatcccact tctaaaacac tagatactct gagtgacaaa
     7201 gccaaatccc tgatgaattt tgccatttcc tcattatgat gtgaggcaaa atctgctgcg
     7261 tacctatccc aatatgcatc gtttagtgct gacatttgca tgggatcatt ttggtatcaa
     7321 aggtacaatc gtgcggacca tcagacatgc cttcggttgg aatctctttc atgcactcag
     7381 aatgtctacc ctttttgcaa aaccagcaaa tctcttcccc ttttccccca actgagcacg
     7441 attccatgat tatcagtatt acaaattcgc taaaaaacct agttctagag gtatctgagc
     7501 cagtgaatga accgctcgtc tcttcccatt gccacatcca taaaggattt ttgcagggtt
     7561 tgtgttgtct gtcctatttt accatcacca ataattacgt catctacttg ggttactgat
     7621 tttacctcag cagcagttcc cgtcataaag atctcatcgg cagaataaat ggcgtctcta
     7681 tccagatctt tttctattat gtctccgcca tcggctcgaa ttatttg
//
